LOCUS       DAVMGZ010000001        62218 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000001 DAVMGZ010000000
VERSION     DAVMGZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 62218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.001"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(92..802)
                     /locus_tag="VYQ93_000001"
     CDS             complement(92..802)
                     /locus_tag="VYQ93_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV3334766.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            complement(805..2496)
                     /locus_tag="VYQ93_000002"
     CDS             complement(805..2496)
                     /locus_tag="VYQ93_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV3334767.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            complement(2508..3518)
                     /locus_tag="VYQ93_000003"
     CDS             complement(2508..3518)
                     /locus_tag="VYQ93_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3334768.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINILTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            complement(3621..4688)
                     /locus_tag="VYQ93_000004"
     CDS             complement(3621..4688)
                     /locus_tag="VYQ93_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV3334769.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDAKEMPKDWT
                     ELASKYKGQYSIYGLTGGTGRAIYASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESTVVKVLDKENAIQYGMIWGSGALVGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            complement(4713..5372)
                     /locus_tag="VYQ93_000005"
     CDS             complement(4713..5372)
                     /locus_tag="VYQ93_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV3334770.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSSY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            complement(5594..5890)
                     /locus_tag="VYQ93_000006"
     CDS             complement(5594..5890)
                     /locus_tag="VYQ93_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3334771.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYKLLEDNKID"
     gene            complement(5874..8033)
                     /gene="pfbA"
                     /locus_tag="VYQ93_000007"
     CDS             complement(5874..8033)
                     /gene="pfbA"
                     /locus_tag="VYQ93_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV3334772.1"
                     /translation="MLKIVKKLEVLMKYFVPNKVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHTIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRENGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKVIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(9150..10376)
                     /locus_tag="VYQ93_000008"
     CDS             complement(9150..10376)
                     /locus_tag="VYQ93_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV3334773.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(10419..11111)
                     /locus_tag="VYQ93_000009"
     CDS             complement(10419..11111)
                     /locus_tag="VYQ93_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV3334774.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWFLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(11284..13035)
                     /locus_tag="VYQ93_000010"
     CDS             complement(11284..13035)
                     /locus_tag="VYQ93_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3334775.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(13025..14770)
                     /locus_tag="VYQ93_000011"
     CDS             complement(13025..14770)
                     /locus_tag="VYQ93_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3334776.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALILVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQTAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYTIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIEQGTHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(14976..15353)
                     /locus_tag="VYQ93_000012"
     CDS             complement(14976..15353)
                     /locus_tag="VYQ93_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV3334777.1"
                     /translation="MKRLIDGKPTLLIKNGNIDPEACRSVGLSASDVSLKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(15425..15881)
                     /locus_tag="VYQ93_000013"
                     /pseudo
     CDS             complement(15425..15881)
                     /locus_tag="VYQ93_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            16015..16842
                     /gene="xth"
                     /locus_tag="VYQ93_000014"
     CDS             16015..16842
                     /gene="xth"
                     /locus_tag="VYQ93_000014"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV3334778.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTVTFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            16854..16997
                     /locus_tag="VYQ93_000015"
     CDS             16854..16997
                     /locus_tag="VYQ93_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV3334779.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            16997..17236
                     /locus_tag="VYQ93_000016"
     CDS             16997..17236
                     /locus_tag="VYQ93_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV3334780.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDLYIYPHKFAVLDPDGYFLRFSE"
     regulatory      17619..17714
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18114..18695
                     /locus_tag="VYQ93_000017"
     CDS             18114..18695
                     /locus_tag="VYQ93_000017"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV3334781.1"
                     /translation="MKLLEDRILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            18695..19957
                     /locus_tag="VYQ93_000018"
     CDS             18695..19957
                     /locus_tag="VYQ93_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV3334782.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPIP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LFGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(20007..20468)
                     /locus_tag="VYQ93_000019"
     CDS             complement(20007..20468)
                     /locus_tag="VYQ93_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV3334783.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(20465..21229)
                     /locus_tag="VYQ93_000020"
     CDS             complement(20465..21229)
                     /locus_tag="VYQ93_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEV3334784.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(21410..21814)
                     /locus_tag="VYQ93_000021"
     CDS             complement(21410..21814)
                     /locus_tag="VYQ93_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV3334785.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VYITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(21884..23365)
                     /locus_tag="VYQ93_000022"
     CDS             complement(21884..23365)
                     /locus_tag="VYQ93_000022"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV3334786.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGISLD"
     gene            complement(23384..24562)
                     /locus_tag="VYQ93_000023"
     CDS             complement(23384..24562)
                     /locus_tag="VYQ93_000023"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV3334787.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPATISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVSLDIQTLGELDEWAVDQYSYLIKDENRLKRVR
                     HAVLENQRTLKAQVALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            24726..25733
                     /gene="galR"
                     /locus_tag="VYQ93_000024"
     CDS             24726..25733
                     /gene="galR"
                     /locus_tag="VYQ93_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV3334788.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTKLTLRESTLNQ
                     E"
     gene            complement(25780..26817)
                     /locus_tag="VYQ93_000025"
     CDS             complement(25780..26817)
                     /locus_tag="VYQ93_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV3334789.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIIIE"
     gene            complement(26818..27171)
                     /locus_tag="VYQ93_000026"
     CDS             complement(26818..27171)
                     /locus_tag="VYQ93_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV3334790.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(27415..28305)
                     /locus_tag="VYQ93_000027"
     CDS             complement(27415..28305)
                     /locus_tag="VYQ93_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV3334791.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            28438..28974
                     /locus_tag="VYQ93_000028"
     CDS             28438..28974
                     /locus_tag="VYQ93_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV3334792.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKDYETTTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLQRELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(29140..29946)
                     /locus_tag="VYQ93_000029"
     CDS             complement(29140..29946)
                     /locus_tag="VYQ93_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV3334793.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(30291..31811)
                     /locus_tag="VYQ93_000030"
     CDS             complement(30291..31811)
                     /locus_tag="VYQ93_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV3334794.1"
                     /translation="MTNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALMAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(31804..32532)
                     /locus_tag="VYQ93_000031"
     CDS             complement(31804..32532)
                     /locus_tag="VYQ93_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3334795.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIENGEFMVLVGPSGSGKTTMI
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLTTGYVLQAIALFPNLTVEENIALIPEM
                     KGWTKEEIAQKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKILLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVS
                     NPETILKAPATDFVADLFGGSIHD"
     gene            complement(32547..33311)
                     /locus_tag="VYQ93_000032"
     CDS             complement(32547..33311)
                     /locus_tag="VYQ93_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV3334796.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTETFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVIPKVEDLTNRGKF"
     gene            complement(33315..33758)
                     /locus_tag="VYQ93_000033"
     CDS             complement(33315..33758)
                     /locus_tag="VYQ93_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV3334797.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            complement(34440..34736)
                     /locus_tag="VYQ93_000034"
     CDS             complement(34440..34736)
                     /locus_tag="VYQ93_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV3334798.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            complement(34758..35822)
                     /gene="pepA"
                     /locus_tag="VYQ93_000035"
     CDS             complement(34758..35822)
                     /gene="pepA"
                     /locus_tag="VYQ93_000035"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV3334799.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            36223..36924
                     /gene="galE"
                     /locus_tag="VYQ93_000036"
     CDS             36223..36924
                     /gene="galE"
                     /locus_tag="VYQ93_000036"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV3334800.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQHDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            37347..38306
                     /locus_tag="VYQ93_000037"
     CDS             37347..38306
                     /locus_tag="VYQ93_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3334801.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            38296..39252
                     /locus_tag="VYQ93_000038"
     CDS             38296..39252
                     /locus_tag="VYQ93_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV3334802.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYILLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILIGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            39249..40001
                     /locus_tag="VYQ93_000039"
     CDS             39249..40001
                     /locus_tag="VYQ93_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3334803.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            40097..41058
                     /locus_tag="VYQ93_000040"
                     /pseudo
     CDS             40097..41058
                     /locus_tag="VYQ93_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
     gene            complement(41283..41525)
                     /gene="yidD"
                     /locus_tag="VYQ93_000041"
     CDS             complement(41283..41525)
                     /gene="yidD"
                     /locus_tag="VYQ93_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV3334804.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFDLTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(41525..42247)
                     /locus_tag="VYQ93_000042"
     CDS             complement(41525..42247)
                     /locus_tag="VYQ93_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV3334805.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(42234..42803)
                     /locus_tag="VYQ93_000043"
     CDS             complement(42234..42803)
                     /locus_tag="VYQ93_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV3334806.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(42822..43550)
                     /locus_tag="VYQ93_000044"
     CDS             complement(42822..43550)
                     /locus_tag="VYQ93_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV3334807.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(43550..44290)
                     /gene="xerD"
                     /locus_tag="VYQ93_000045"
     CDS             complement(43550..44290)
                     /gene="xerD"
                     /locus_tag="VYQ93_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV3334808.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(44281..44742)
                     /locus_tag="VYQ93_000046"
     CDS             complement(44281..44742)
                     /locus_tag="VYQ93_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV3334809.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(44739..45260)
                     /locus_tag="VYQ93_000047"
     CDS             complement(44739..45260)
                     /locus_tag="VYQ93_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV3334810.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(45236..46207)
                     /locus_tag="VYQ93_000048"
     CDS             complement(45236..46207)
                     /locus_tag="VYQ93_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV3334811.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(46204..46998)
                     /gene="racE"
                     /locus_tag="VYQ93_000049"
     CDS             complement(46204..46998)
                     /gene="racE"
                     /locus_tag="VYQ93_000049"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV3334812.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(47205..47453)
                     /locus_tag="VYQ93_000050"
     CDS             complement(47205..47453)
                     /locus_tag="VYQ93_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV3334813.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(47709..49334)
                     /gene="treC"
                     /locus_tag="VYQ93_000051"
     CDS             complement(47709..49334)
                     /gene="treC"
                     /locus_tag="VYQ93_000051"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV3334814.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(49512..51479)
                     /gene="treP"
                     /locus_tag="VYQ93_000052"
     CDS             complement(49512..51479)
                     /gene="treP"
                     /locus_tag="VYQ93_000052"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV3334815.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVSHELVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETLVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            51664..52374
                     /gene="treR"
                     /locus_tag="VYQ93_000053"
     CDS             51664..52374
                     /gene="treR"
                     /locus_tag="VYQ93_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV3334816.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(52407..53210)
                     /locus_tag="VYQ93_000054"
     CDS             complement(52407..53210)
                     /locus_tag="VYQ93_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3334817.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(53223..54116)
                     /locus_tag="VYQ93_000055"
     CDS             complement(53223..54116)
                     /locus_tag="VYQ93_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3334818.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIVEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            complement(54128..54859)
                     /locus_tag="VYQ93_000056"
     CDS             complement(54128..54859)
                     /locus_tag="VYQ93_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3334819.1"
                     /translation="MGILLTSLISLHQLKADKKRDVFRIGISQFITHQSLDATREGFV
                     DELAKQGYVEGENIEIDLQNAQGEQRNLKTISQQLAESSDVVLAIARPSAQSEPNSVV
                     QKDEAKRLLKEKGFTVVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISLTMETVKQ
                     VSIKHKVPVFGGSTEMIAVGGLYNYGTNYEELGRQTARMLIHVLKGEKPENIAVELPE
                     KLELHTNQEMADALGIDISKLEGKE"
     gene            complement(55349..56275)
                     /locus_tag="VYQ93_000057"
     CDS             complement(55349..56275)
                     /locus_tag="VYQ93_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3334820.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(56286..57353)
                     /locus_tag="VYQ93_000058"
     CDS             complement(56286..57353)
                     /locus_tag="VYQ93_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3334821.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGELYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            complement(57362..58288)
                     /locus_tag="VYQ93_000059"
     CDS             complement(57362..58288)
                     /locus_tag="VYQ93_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3334822.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            complement(58288..59784)
                     /locus_tag="VYQ93_000060"
     CDS             complement(58288..59784)
                     /locus_tag="VYQ93_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3334823.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            complement(59851..61830)
                     /locus_tag="VYQ93_000061"
     CDS             complement(59851..61830)
                     /locus_tag="VYQ93_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3334824.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
BASE COUNT        18576 a        13859 c        11303 g        18480 t
ORIGIN      
        1 acctctgagc aaaaatctac tcaaaaatta gatgattaaa acatctaaaa aacaaaagga
       61 caaaaacatc tgtccctttg tttactaaat ttcagctaat ttcttcgaca taaataacac
      121 ctacaatatt agcaatttct tccatcagtc gaagatgttc aaatctacct gataattcca
      181 gagtaataaa tgacgctatt tttttgtccg gaacatcaaa gtattcaatt ctgtcagaat
      241 taacatctcc aaacgctgtt cttgaatcgg tcattctgat accattttct gcacaataaa
      301 ccaatacacg attataggct tctgtagatt taaccactat atacaattca atcattttag
      361 aacgattttg cagatatttt tttagtggtt ggaacatgga tatcacaccc caaacagaaa
      421 tggctactaa aagagctccc tcataaaaac ctactccaat agctaatcca attcctgccg
      481 aagcccaaat gcctgctgca gttgtcagac ctgtaatttt ctttttatct gtaataagaa
      541 tcgttccagc gcctagaaaa cccacacctg atataacttg agctcctaat cgtgtaggat
      601 ctcctgtccc aaatttataa gatacgtatt cattcgtcat cataatcaaa catgcagcta
      661 gacaaacaat actataagtt cggatgcctg caggctggga tttgctccct ctctctaaac
      721 caattatact accaatgact actgataaaa caatcctgac aactatttca atatttgata
      781 acccaagact agtggctgtc atgattattt ccttacttta cgccccggtc tttgtgtgaa
      841 gtataatacc gttccagaaa taatcatcag aacaattgta taaacaaata ccagagcttg
      901 tgcattagat gttgctgttt catcacctgc agatcgaatc gtaataccta atggttgagc
      961 taggggatgg taaaggaata cagataagtc gaagtcagtt aataaagagt taaagtttaa
     1021 agcaataaca gagagaacaa ccggtaaaat aaatggaatg ataaccttca tcatagtata
     1081 aaaaggtgaa gcacccatac ttcttgctgc atcttccatc tcatcatcaa cactaaataa
     1141 aatagcacgt accattctat aagaaaatgg gatttttaca actatatatg caataagtag
     1201 aattaccaaa ctacctacca aaatctgatt caagacaaga aattgtggct gattaaaagt
     1261 aaataataaa cttactgcta aaagcgtact tggtagtaac caaggaagta gagcaccata
     1321 ttcaaataag aaatcaaaac gagatttatg ttttctgaca acacgagcaa atacaactgc
     1381 gagaattgtt gctgttgtcg cagcaataat agaataaata aagctgacca agaatggaga
     1441 gaatgccgca ctattactaa agaataagcg ataattttct aaagtaaagt ttgataatgt
     1501 taagttacct gtttgaattg caactggatc tgtaaatgag tataatacta taaaaattag
     1561 tggaagcatg aaaactgtga acaatccata tgctacaatg tgagcaatga tattccaagg
     1621 cttagacgca attttttgtt ttttaagagg cgctttagtc ttagagatag aaatataatt
     1681 tccacctttt tctatcttat tcatgatagt aagcaaaatt gtagttgcaa tacctaaaat
     1741 aattgcaagt aaggcagcta aatcacgaga attccccatc cctgcaaatg taataatcat
     1801 tggatttata gtttgaaatt ctttaccacc aacaatcatg ggtgctgcta ctgcagataa
     1861 accactaaga aaaaccataa tagtaagtgc aaatagagtt ggaattaagg ttggtaacac
     1921 tacttttcgg aaaacagtaa atggttttgc tcccatattt cgagcagcct caatagtgtg
     1981 atagtcaacg cttcgaattg tatttgttaa aaacaatgta tgattagcag ttcctgaaaa
     2041 tgtcataatg aataagactg caccataccc aataaaccag ttagagtcta aagaagggat
     2101 aacattttgt aaaaattttg taatcaatcc ataaggacca tagacaaatt tatatccagt
     2161 cgctaaaacc actcctccat aaattaaaga ggtcatataa cctaatttta aaattttagc
     2221 acctttaata tcaaagtact ctgtaaatag aacacaaaga atacctacga cattaactgt
     2281 aataatgagt gaaaatgcta acttaaaact gttcataata ctctgaagtg ccctctgaga
     2341 ttttagaaca cgatgtacag catcaaggga aaattctcct ccttttacaa atacattcac
     2401 tactagatca aagtttggat aaataataaa tgttactaag aaccagatta accctaaacg
     2461 aataagccaa tcttttaaat ttaatttatg acgcatactg cacctcctta aaattgcaga
     2521 acgtctgatg gtgtgataaa taattccaca ctttctccga cagatctaat agcagcctga
     2581 ctatcaatac ttgttacatt aagaatctga ctttcagaaa cttttattgt atagtgaatt
     2641 gtaactccag aaaactcaac atcaataatt gtccctttta gaataaaatc ttgttcagtt
     2701 tcacgattga atcgaacttt ctctaatcga atgtatcctt ttttatcctc taagaaaacg
     2761 cttgtatttt tcaataatac ttcgtggact gtttcatcgg tcaaaatatt aatatctcca
     2821 ataaaatcac atacaaattc agtttgagaa ttatgataaa tctctactgg tgtaccgacc
     2881 tgttcgatgt atccattgtt aaagactgca attctatcag ataaagtcaa ggcttcctct
     2941 tgatcatgag taacatataa agtagtaata cctaactctt tttgaagtct tttcaactct
     3001 tttctcaaat ctacacgtaa ttttgcgtca aggtttgaca atggttcatc tagacaaaga
     3061 attttaggtt caagaaccag agcacgagcc aatgctaccc tttgttgttg acccccagat
     3121 aattctgata cattacgctg taactgttga tcagagatct taatttttgc tgccactgct
     3181 gatactttag ctttaataac atctggagct accttcttaa cttttaaacc aaatgcaata
     3241 ttatcaaaaa cagtcatagt tggaaatagc gcataagatt gaaatacaat accaattcca
     3301 cgcttttcag gttccaaatg agtgacatct gttccattaa cttcaatact tcctgatgat
     3361 ggatctaaaa aacctaccaa tgctctcaaa gtagttgatt taccacatcc tgaaggccca
     3421 agaaatgtaa agaattcccc ttcatgtata tctaaattca gattatcaat tgcaacaaaa
     3481 tcaccatatt taatttgaat attatcaaat ttaatcatct cactaactcc ctctattact
     3541 aaaccaaaag cctctcttta tttcttcctt aaatttagaa ataatagaga gacttggaca
     3601 taaaaaataa ctcttatttc ttattgtacg tattctaatt cagctttttc tacccattca
     3661 tccaaatgct ttccaacagc ttcccagtca atattttgtg gtttcacttg atcaacaaat
     3721 tttttcgtat cttcaggtaa ctcttttagg gcatctttat ttgcaggaat agatccaaat
     3781 tttttactat attctacttg aatttctgat tgaccaaacc aatcaataaa ttctttagct
     3841 aacgcttgtt ttttactagt gcttaaaacc atagtttgtt cagttacaaa tggtacacca
     3901 atctcaggag tcataacttt gaattcaaca ttttgttctt tttgaccaac tagagccccg
     3961 gacccccaga tcataccgta ttggatagcg ttttctttgt ctaatacttt aacaactgtg
     4021 ctttcacctt tttgtagtgt ataggcattt gcaaagtatt cttttgcaac ttcccagccc
     4081 ttttccgata ctcctaaatc acctttttca tcaagataac gaactagaat acttgcgtaa
     4141 atggcgcgtc cagtcccacc agttaagcca taaatggaat attgtccctt atatttacta
     4201 gctaactctg tccagtcttt tggcatttct tttgcatcag gagctccaat caataccaag
     4261 ggttgaacaa taactgggtt ataataatta tctttatctg ataaagattg atcaatttta
     4321 tctaaccatt taggcttgta ctgtactagt aatttttgat ctctaatttt atttgagtca
     4381 acagcaccaa ttccaaatac catatctgca actgcattat tcttctcagc aataacacgg
     4441 tctgctaatt gagcgccagc gatatcaacc atttttatat taaaaccagc ttcttttgct
     4501 ttagcagtta accaatcacc acgaccattt gagactgagt tcgaatagat tactaattct
     4561 tgacttttat cagctttttc ttcagatgaa gaagcagtcg tagaatttga acctccagag
     4621 caagcagcaa gtgtagtaag agcaactccc gttgcaagta cagtagacca aactttcatt
     4681 tttttcatga taagttctcc ttttttatta ttttatttaa atttttcgtg atatggaaca
     4741 aattgtctca tatcttcaaa tacagtatag tcaatacggt ttacagtaat agttggaatc
     4801 ttctctaata aaatttcagt taattctgct ctgactttag taaactcttc ttcctcctct
     4861 tcggttagag gaatccgaag atacccaatt gaaatatgga attgatatct atcatgatta
     4921 gggaaacaaa cacctgcttt ttctgagaca taagtacgaa tttcttctaa tctctttgca
     4981 gaagcttcat ctgcaggttc aactagtatg ttttgttttc ccatttcagt tatacgcata
     5041 tgaatttctt catccaacaa tggaaaaatt tcaagttgtt tagcaaagta atcatgtatt
     5101 tcctgtaaag gtgtatctag aggaagatta ctgctccaaa actccgtttc acgattttca
     5161 tggcacaaca attcaattac agtcatgtga atagaattcc ttggagttaa agtaaactta
     5221 tcgataaatg gtaattctct ataacttgat tgaataatat caacaacttc catcaaatct
     5281 tgtttagtat aaagatttgc tacaactgta ttcccaggga aatgattaaa ttccccattc
     5341 tctttgaact taatttttgt taagtttttc atataaagac cttctttttt atatttgaca
     5401 tcggtgtcat cgtgtttaca ttatacaatg aaaccgtttc ctttgtcaag cgctgtcaaa
     5461 catttttttt aatttatctt tcattaaaag gttaagtaat ttttccaaaa tacagcactc
     5521 atatagacag tatcatcaat tatagccatt gggatttttt gattgccaat tccatgtatc
     5581 tctacacatg tcttcaatcg attttgttgt cctccaataa tttatatgag tttaaaccga
     5641 tttcatcttt aacctcgagt aaagcagttt caaatatttg tttaagagtt tttgattctt
     5701 tacaattaac cgacaaactt tctgataaaa tatgtacaac ttctgagact gaataaccta
     5761 tctcctcttt agaattatat aaatctgtag ctccaccaat aatccaaaaa tactgatttt
     5821 gtgaacctac aatatcctca ttttctacgg aacttccttg tatcgaacaa attttattta
     5881 tctttaccat aatacttcaa cccttttagt gtcaaaagta aaccaattcc tgtcactgtt
     5941 aagaatagtt ccataatctt gttcgaacca gtctttggta atttttgttt tacatctact
     6001 atttctttag atttattaat atgattttca gtttctctgc catctccaac tattttatag
     6061 tttacttctt ctgtcttatt atcttgttta ttgtcgatct tgtcattcat ttgtctatta
     6121 tctttacttg agttaaactc tccgttcttc tggttactat caattacatt atttgaatta
     6181 gattgttttt cctctttgtt tttttctttt tcgtttttat cacttaaatt atttgttaca
     6241 attttgtaaa gcccattctc cgttacaata ttgaaattac catcgctatc acgtataaca
     6301 ggttctttcc catttgcatt agatttgatg aatgatatat acttaccgga taaattataa
     6361 aattggttat ttaaaacggt tattttaccc tttgaatcct caataacaat tccttcttta
     6421 ccaccgaata tactattctc tgagactact aaattatcac taactcgtaa taattcaata
     6481 tttggttgtt ctgtttcctt agaattatta ttaattacat tttttgttac agtaatatct
     6541 gatacttttc ctaaatattc tgcactatct tttgcaactc gtatcacttt tgttttagga
     6601 tcggcaatat taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct
     6661 ttagtgtttt tatagctata agcatttact aaagctgctc cgttttctcg ataatgtaca
     6721 ctctctcctt taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct
     6781 gtaaaacgta cacctgcata catcatgtta tcaaaatgat tatttaaaat tttaatatta
     6841 gaggggttct gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca
     6901 gatttatcac ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca
     6961 tcatcattca aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc
     7021 ttacttatga tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa
     7081 ttatcaacta atacattttt cgaacctgca atttgaatag tatgcccttg ataactatcc
     7141 ttgaatgtta cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa
     7201 gaatttataa gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata
     7261 tcaatcgtac caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca
     7321 tcatctgtaa ataaacctgt cataaaaaca atggaagggt gattcttaat atttatacca
     7381 tttagaattg tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt
     7441 tcttttacta aataagttcc ttcaggaaaa tatacatttc ctccacctag cccttgagct
     7501 gcagcatcta ttgcatcttg aattgcttgt cgatcatcat gaatcccatc acctaccgca
     7561 ccataatctt ttacacttaa agcagtatct tcaaattctt ttttgataat tctgtcactt
     7621 ataacatctt ccttataact cttattttca ttagtagatg ttactgtaga ttcaactgtt
     7681 ctgttgtcta attgtgaatt ggaacgaata gtaccattag agctgatact attaacactt
     7741 aacgaattat ctaaaccatt agtggttgaa ttagaggcaa tcatttcagc agaacgattt
     7801 tgattaacag ttggtgaatt atctaaatca ttagtaattg cattagaaga ctcttttgta
     7861 gccatcggtg aagaactagt aacaacttca tccgataaaa taccttggct tcccaaaatt
     7921 gaaattgcca atagtaccga gcaagtcccc acctttaatt tacgaatact gaatacctta
     7981 ttaggaacaa aatatttcat cagaacctcc aattttttta ctatttttaa caattgatta
     8041 ttacttttct taaaatagat agaatgacac cgctgtcatt atctttatca tatattttcc
     8101 aaattgaatt gtcaaggaat aacacaagaa aatattaaaa tttatactct tcggaaatct
     8161 cttcaaacta ggtcagtgcc gccttgccct aagtctaggt tactgacttg gtcagttcta
     8221 tagtagactg aaactataat agtacacctc tacttctaaa acattgttag aaatcgattt
     8281 aactgtcccg attaatttgt cctattctta tttcatttta ttatatctac aacctcaaag
     8341 cagggttttg agctatctat ggccaacttc ctagtttgct ctttagcctt ccattcaaaa
     8401 gtgatgctat ctttcttagc ttgaatataa aatatcccat ttactatatt taaatttgtt
     8461 gatatgttgt aaattataaa tgaaagaaca aatatcttgt catacaagca ttcgccaggg
     8521 atatttacat gaatatcgct catatggcgg attgaaacta aaacactaca atatatttct
     8581 taaatagacc ttctataaaa gctagattta acctcgataa aaatagatgc agtgcttcta
     8641 aaatactgga atagggcata taggtaatcg tagacaagtg ttttatagat tgtctcgcag
     8701 gagatgcaag catcactaaa catatagagt atttgtatta ttataatatt aacattctat
     8761 aaattctatc atgcaaaaga atgactagaa catgaaagac aggcacaaaa ctttgaacaa
     8821 tattctgaaa acaaaaaaag acgcgagttt ccacaatctc actcatggaa actcacgtct
     8881 ttcaaaaaat agggtgaatt gaaaactaga acaagacact agattttcta aatgatgtct
     8941 agtaaggatt ctaaatgcta caccaaattg ctcacatctt atttcaaacc acgggactcc
     9001 cacaagaaag gaaaatccta taaaaattct ctctctgagg agaaaggagc ctcaactaca
     9061 ggtgtaaact attatcaatt gaaaacaaca gtttttacac tcaattagac tttttaggga
     9121 caggatcact ttcgcttgac tgtagtttgt catttttttg ataaagttag cactttttca
     9181 gaaactgttt tgaaagtctc aatgatatag tctacttctt catcgcttaa tttagtatga
     9241 agagggaggg taatttcatt ctcaaagaag gcataggcct taggatagtt cgtcatatca
     9301 aatccaagat tcttataggc tgtcaagaga ggaagcggtt tgtagtgaac attacttgca
     9361 attcctgctt tagccaattc ttggatgatg aggttgcgtt cttctaggct tgctccttct
     9421 acacgggtga tgtagaggtg gcgtgaagat tcgacagttt cagtcttgtg tgccaaagga
     9481 tggatgcgag aacctgcaaa accactatca tagcggtcca caatgtcctt acggcgttgc
     9541 aacaaacttg gatagcggtc caattgtacc aaaccaagtg aagccatgat atcggtcatg
     9601 ttgcacttat aggctggtgt aacgatatcg tattcccatg accccaattg catcttggca
     9661 agagcatcct tagtttgccc gtgaagggaa aggatttgga attccttgta catctcttcg
     9721 tcatcaatca ctggattggc tttccaagtc gcacttccac cttctgccgt tgtaaagttc
     9781 ttaacggcat ggaatgagaa ggaagtaaag tcagcgatag aaccagaagg ttgtccttta
     9841 taagtagatc ccaaagcgtg ggcactatca gagacaatga caatacggtt aaaggccttt
     9901 tgccacttgc ttgaagcagt aaagaagtca cgttttttct ccacgacttg gaacaaacgg
     9961 tcataatcgc aaacaatccc tgcgagctct actgggataa tcaccttagt tttctcagtg
    10021 atagcttgct caagcaggtc atagtccatc tcaaacgtat ctgcttggat atccaccatg
    10081 acaggggttg ctcccacgtg cgtaatgaca ctacatgaag ccgtataggt catggctgga
    10141 acgatgactt catcaccagg tcccacttcc aaaacgcgta aaatcaactc cagagcggct
    10201 gtcgcagagt tgagacaaac agtcttaggt gtctgtgtgt aaagagacaa gcggcgctcc
    10261 agttcttttg ttttaggacc tgttgtgatc caaccagaac gcagggtatc cgctacttca
    10321 gcaatttctg cttctgtgat atcaggcggt gaaaatggaa tattgtaatt tggcattgat
    10381 ttgctccttt tatctgtact cattttctta tgactacttt attttagtac ctcaaacacg
    10441 gtttgaaaca tgattttgat gtccccaaag aaactaaact ctcggagata ggcgaggtta
    10501 tagcgcatct tttcaggaag aacatgctcc acataggcct gatcaactga cagacctttc
    10561 tccgtcattt gactgatgat ggtgtcctca tccttgtagt tgatgctggc tggagaggta
    10621 attcctgctt gcaagagcaa ggttgccatc atttcagggc tatactgctc tgtataacgt
    10681 ggcacttcag gtcgtgtacc gacaaaggac atctcacctt taaggacatt gaccaactga
    10741 ggcagttcgt ccaaacggac acgtcggatg aaatttccaa ccttggtaat acggctatcg
    10801 ttagcagaag tcaccagact tcctttttta tccgcatccg tcaccatggt acggaacttc
    10861 caaatcttga accgacggtt gtactgggtc acacgctctt gcttgtaaat cactggcccc
    10921 ttgctatcca acttgatcca aatgctcaag atgagaaaga tgggagaggt tagaaccagt
    10981 aagaccaagg ccaaaaacca gtccaagcaa cgcttgaaaa tcagcgaacc cttcctttta
    11041 gagacaagct ggtagtaaga ctcaacctcg cttgatttca tttccacagg caagtcttcc
    11101 catttcagca tgctgttctc ctttgttttt attatactat ttgtcaacat ttttcattat
    11161 accacaaaat ggaaaaggcg gtgaaagaaa tctgtaattt gaaggatttc cttctagtac
    11221 tcaatgaaaa tcaaagtaca gctttgagat tgcagataaa gctgacatgg tttaaagaga
    11281 ttttcaaaga gtataggcca tggcccctgc ctgcaaacta tacatcttgt gataggttcc
    11341 tcccagagtc aagagttcct catgggttcc actctcgata atgcgtccct tatccaagac
    11401 atagatgcag ttggcgtctt gaatagtaga aaggcggtga gcgatagcaa tagttgttcg
    11461 gccctgtctc atcttcgcca gagaagcttg aaccaaactt tctgtttcag agtcaatatt
    11521 ggctgtcgct tcatccaaaa tcaggatttt aggctggctg gcgactgttc tagcaaaggc
    11581 aagaagctgg cgctgcccag tagagaagct cgaaccacgc tcggaaacag gggagtcgta
    11641 cccctgagga agttcttgaa taaaggaatc tgcatccaca aaggctgccg cagcctgaac
    11701 ctgctcatca ctgatttctt ggtacatggc gatattggac ttaatagttc catgatagag
    11761 gaagggttcc tgtaagacca aaccgatgtt ttttctcagc tcttcttgac tgaaatccct
    11821 gatatccaca tcatccaaga gaactctccc tgactggaat tcataaaagc gcatgaggac
    11881 attgataata gacgatttcc ctgaacctgt atgacctaca aaggcaatgg tttcaccctt
    11941 attaacagag aaagaaatgt catccagaat cggatgttta ccgtcatatg agaaacacac
    12001 atgttcaaaa cggatattgc cttcttggac tttggcttgc ccattttctt gaagaggttc
    12061 ataggtcctc tcgtctatca gggcaaagac acgacctgca gaaaccatag ccgtttgcag
    12121 agttgaaaag ttttgcgtca cctcaatcaa ggggtcaaaa aggcggttga tgtactggat
    12181 aaaggcatac atggtcccga ccgttatccc gatagaaaaa ccacggtagc caaagtaggc
    12241 catcaagact gcatagccta gaagtttcag caaactcatg gcaggtctca aaaagagggc
    12301 atccaaggct acagaacggt tggcgtagac caagtgttct tggttgattt catcaaattc
    12361 tgcctgcagg cgcttctctt gattaaaggc ctgaataatc ctgattccct cgatattctc
    12421 tgccagctta ctattgatat ctgacaagag acttctggtt ttctcgatga ttttcactga
    12481 cttttttcga tagagattga ccaaaaggaa aatcaaagga agaaagagca agactaaagc
    12541 cgtcaaacga aaatccagca ccaacatggt ataaagggtt gtcagaaaga taaaaactgc
    12601 tgagataaag ctggataaaa tcccagaaaa catatcactg atagtctcgg tgtcatttgt
    12661 caaacgagag acgatagagc ctgctggcgt cttgtcaaaa taagacatgc ccaacttttc
    12721 catattggca aaggcatccc gacgaatatc cctaacaata ctgtaagaca cgcgcgcaaa
    12781 gagaagattg ccgacatact gaactacagt ttgtaggatg tagagaccat agtagaccag
    12841 caaaacggta acggctagtt ggttaagatt gctgagatac tggtcgataa agtgggaagc
    12901 cacgaggggt atgacacttt taatgaccgt cgtcgctagg agaaaactga gtgccaaaaa
    12961 ggtcaggagt ccataaggct tgagataaga catcaagcgc ttcaatacag cccattgttc
    13021 ttgtttattc tgcatcttct tctcctttca tttccaactg ctgagactgg taggtttggg
    13081 cataccagcc atccatcgct agcaagtctt cgtgcgtacc ttgttcgata atttgaccat
    13141 tttgtagaac taaaataaaa tctgcatgga caacagcact gaggcgatgg gcagtgataa
    13201 tggttgtctt gtcctttcgc gtctccttga ggttgtcgat aatcgcatac tctgtcttgg
    13261 catctacggc ggataaggaa tcatccaaaa tcaagatatc agggtctaaa atcatagccc
    13321 gactcatagc caaccgttgc ttttgaccac cagaaagact gactcctttt tcaccaatca
    13381 gcgtatcaaa tccttgaggc atgtctacaa tatcttggta aactcgggct agcttagtag
    13441 cttcctcgac cgctgaaagg ggcaagttag gattgccaaa gcggatattg tctaggattg
    13501 aagtcgcaaa gagaaactgg tcctgaggaa catagcccat gagactgcga aggtctgtca
    13561 gacgatagtc ccgaatatcg tgaccgttta gataaatggc acccttatcc acatcgtatt
    13621 cacgcaagag gagcttgatt aaggacgttt tcccagagcc tgtctgccca accaagccca
    13681 gtgtttgccc ttttgccaaa ctaaagtgaa tatccgtcag tgtttcctca ttttcaaaag
    13741 caaagctgtc aatggtatac tccaaacgcc cattttcaat accatccaga ggaaactcag
    13801 ggtcttgtac aggagattcc tgagacaaaa gattttcaat ccgctggtaa gaaaccttcc
    13861 ctcgctgagt agtattaaag aggaagccga tggccataag aggccagacc agcatatcca
    13921 aatagctgat aaaggtgact agattaccaa ctgtaatctg cccttcctga accatcaagg
    13981 agccaaccaa aagcgttaaa acataggacg aaccaacaaa caagagaacc atagggtcaa
    14041 agagactatc atatttcatg gtttgcaggt tcttttggaa ggttaattca ttgactgcct
    14101 gaaaagactt caactcgtct gcctgataac cgaaagactt ggtcactttg atacctgata
    14161 cggactcctg taccttgtta ttgagttcag aaaaagcagt ttgggattcg ccaaaggcct
    14221 tatgagtctt tctccctagg cgactagtcg cataggccat gaaaggtagg gggagaatgg
    14281 caacaagagt catctgccat gagatgctaa agagcatggt caacaaagtc accagagccg
    14341 tgatagaggc atccaccgca gacatgacac cgccacctgc taaacgagtc aaggcattga
    14401 tatcattggt tgcgtgtgcc atcagatcac ccgtccgata ggtttgataa aaggctgacg
    14461 acatttttgt gaaatgctta aacaagcgag accgcatgat ctgtcccaag caataagagg
    14521 tcccaaggat atacatacgc cacacatagc gcaaatagta cataccaaag gctgcaagta
    14581 gcaagtaaaa taggctaaga aggaggtcct gctgggttaa ttgccccgat gtgatggcat
    14641 caataacccg ccccataacc ataggaggaa tgagattgag gacggaaacc aagatcaggg
    14701 ccacaattcc gactagataa cggcgttttt ctaacttgaa aaaccaccaa agtttttgaa
    14761 taatggacat aaaatccctt tctgattgca aatggaaacc tgaggccaat actcaatgaa
    14821 aatcaaagag caaactagga aactagccgc aggctgctca aagcactgct ttgaggttgt
    14881 agatagaact gacgaagtca gtaacctaca tacggcaagg cgacgttgac gcggtttgaa
    14941 gagattttcg aagagtacaa gaccccaggt ttttcttatt cataagttac gactgtaaca
    15001 gcacccttgt catattcagc aataaagata ttggctacat tgtcatgccc ttgtttactg
    15061 aggttatcaa gcaaccactc ttcgctacga ccaatcgatt ccaagacatc tacttgaatc
    15121 acaccgtcag tcacaactgg atacttagga ttttcatctc ccatttgcac aacgatgagt
    15181 tgcccatttt gctcttgcac agctcgtttg acttgcttca tctggaaaat cccttggcta
    15241 cgaagtttga gggatacatc cgatgcagac aaaccaactg aacgacaggc ttctgggtca
    15301 atattcccat ttttgataag gagagttggc ttaccatcaa tcaaacgttt cacaaagtga
    15361 acattgttgt tgagctattt gagagtcaag accagtttga ttaaaatttc gatataatta
    15421 agtgtcattt gttcacatcc accagttcaa ttgcgtctgt cttatacaat tcgactttct
    15481 ctaacaggta cttgtctggc tcacttccgt ttagggcacg ataatagcga tctcccacct
    15541 tgataagtgc gccatctgtg tttgtggaag tattaacata gacttctgac ttgtctactc
    15601 ccaaatcttt ggaaacaacc tcgatgaaat gaagtgaagt ttgaaattta ttgttagaaa
    15661 cttgattgtt ttgataagtc gtgatgctga ccaaaagcag agctaaataa ggccagatct
    15721 gaaatcatga ccaactcacg aaattcgctt ccctttttat catggtatgc cttgaaagca
    15781 aaaaaaactg tcacagctaa tagaacaatc ccaaagccaa ccatgatacc attttgctga
    15841 ccgatttggc tgagtacata gtcataagag tagaatttca tgtattttca ctccctttca
    15901 taaaatcatt cttaattata aacgaaagag agtaattttt caaaccatac gttggggaaa
    15961 tagatctttt tttggtataa taaaatctat aatctgaatg aaaaaggtaa ctttatgaaa
    16021 cttatctcat ggaatattga ttccctaaac gctgccctaa ctagtgactc agctcgtgcc
    16081 aaattgtccc aagaagtcct acaaaccttg gtcgctgaaa atgctgatat cattgctatc
    16141 caagaaacca agctttctgc caaaggacct acaaagaaac acgtggaaat tttagaagaa
    16201 ctcttcccag gctacgaaaa cacgtggcgc tcttcccaag agcctgcccg taaaggctat
    16261 gctggaacca tgttccttta taagaaagaa cttacaccca ctgtcacttt cccagaaatc
    16321 ggtgcccctt ctaccatgga cttggaaggc cgcatcatca ctctagaatt tgatgcattt
    16381 ttcgtgaccc aagtttacac accaaatgct ggcgatggtc ttaaacgctt ggaagaacgc
    16441 caagtctggg atgtcaaata tgcggagtat ttggctgaac tagacaaaga aaaaccagtc
    16501 cttgcgaccg gtgactacaa cgtagcccac aatgaaatcg accttgcaaa tcctgctagc
    16561 aaccgccgtt cacctggatt tactgacgag gaacgtgctg gatttaccaa cctattggca
    16621 actggattta ctgatacctt ccgccatgtt catggcgatg ttcctgaacg ctacacttgg
    16681 tgggcacaac gcagcaaaac ttctaaaatc aacaatacag gctggagaat cgactactgg
    16741 ctcacaagta accgtatcgc tgacaaggtc actaaatctg atatgattga ctcaggtgct
    16801 cgccaagacc atacaccgat tgtcttggaa attgacttgt aaggattttc tatatggact
    16861 acaatgcggt cattcccgag tttcttgtat cgaatatcga acagtcccgg tccttctact
    16921 gtggtttact gggttttagg attgaatacc aacgtccaga agaaaacttt ctctttctct
    16981 tgaagagtgt caactaatgt tagaggaggg aacaaaggat caactagctg aactgaccta
    17041 cccctttggt cgtggagtca atctctcctt tggcataaag gatgttccaa aactctatca
    17101 aaaagtgatg gaggcaaact atcctattta tcgtcccttg accaaaagaa agtttcgtgt
    17161 cagtgatctc tatatctatc ctcataaatt tgcagttttg gatccagatg gctatttttt
    17221 aagatttagc gagtagaata ataaaaattg ggaaaagtct ttaaagttag aaaaaggcat
    17281 attgtcaggt gtctaaaaac ttgatagtat gccttttatg atggaaaaat cttctaatac
    17341 tcttcgaaaa tctcttcaag ccacgtcaac gtcgccttac cgtaggtata tgatactgac
    17401 tctgtcaatt ctatccacaa cctcaaaaca gtgttttgag gttgtggata gcttcctagt
    17461 ttgctctttg attttcattt agtataaatg ttagcttaca atggagtttt tgttcaccct
    17521 ctcttctgtt tgttcgtttt ttaaaccaaa acactaacaa aatagttgat ttctaaagtt
    17581 atttagtgta aaataaaaac attggctcaa gcctatttaa aattgaatat cgttttactt
    17641 gtttatgtcg tgaattggca cgacgtttct acaaggtgcc ggaacaccta acaataagta
    17701 agtcagcagt gagatatggc cgtttttgcc atgtctattt aggggactta cttagagatt
    17761 aagtaggtct tttttgaata tttttaatat ttcccttaat tagtgcggac gagaggtgaa
    17821 attatcctgt gaataatttc aggaatccag ggaatttcat ccctaatttt tgagccccta
    17881 gctctttcat ttctaggctt aggctaaaat agttccccga actattttac tctcaaaaat
    17941 cccgtcatag acaataacaa gattattgtc tatgatacca ctacaacggg aaatatttga
    18001 taagactatt acgagcgaag cgagtcttat caaatatata gtattagcaa aagggatcac
    18061 tatagaaaca tttcaacaac aagtaaatta gcttttagac tttaggaggt cttatgaaat
    18121 tattagaaga tcgcatcctc aaggatgggc atatcttggg tgataacatc ctcaaggtag
    18181 attccttttt aacccaccaa gttgacttta gcttgatgcg agagattggt aaggtttttg
    18241 cggaaaaatt tgctgctgcc ggcattacca aggtcgtaac cattgaagcg tcgggtattg
    18301 ccccagccgt ttttacagct gaagccttaa acgttcccat gattttcgcc aaaaaagcta
    18361 agaacatcac catgaacgaa ggcatcttaa ctgctcaagt ctactccttt accaagcagg
    18421 tgaccagcac cgtttctatc gctggaaaat tcctctcacc agaggacaag gttttgatta
    18481 tcgacgattt ccttgctaat ggccaagctg ctaaaggctt gattcaaatc atcgaacagg
    18541 ccggtgccac agtccaagct atcggtatcg tgattgagaa atccttccaa gatggtcgtg
    18601 atttgcttga aaaagcaggc taccctgtcc tatcacttgc tcgcttggat cgttttgaaa
    18661 atggtcaggt cgtatttaag gaggcagatc tctaatgcaa actcaagaaa aacactcgca
    18721 agcagccgtt cttggcttgc agcacttact agccatgtac tcaggatcta tcctggttcc
    18781 catcatgatt gcgacagccc ttggctattc agctgagcag ttgacctacc tgatttctac
    18841 agatatcttc atgtgtgggg tggcaacctt cctccaactc caactcaaca aatactttgg
    18901 gattggactc ccagtcgttc ttggagttgc attccagtcg gtcgctccct tgattatgat
    18961 tgggcaaagc catggtagtg gcgctatgtt tggtgccctt atcgcatctg ggatttacgt
    19021 ggttcttgtt tcaggcatct tctcaaaagt agccaatctc ttcccatcta tcgtaacagg
    19081 atctgttatt accacgattg gtttaacctt gatccctgtc gctattggaa atatgggaaa
    19141 taacgttcca gagccaactg gtcaaagtct cttgcttgca gctattactg ttctgattat
    19201 cctcttgatc aacatcttta ccaaaggatt tatcaagtct atctctattt tgattggtct
    19261 ggttgttgga actgccattg ctgctactat gggcttggtg gacttctctc ctgttgcggt
    19321 agctccactt gtccatgtcc caattccact ctactttggg atgccaacct ttgaaatctc
    19381 atctattgtc atgatgtgta tcatcgcaac ggtgtctatg gttgagtcaa ctggtgttta
    19441 tctagccttg tctgatatca caaaagatcc aatcgacagc acgcgccttc gcaacggtta
    19501 ccgcgcagaa ggtttggccg tacttttcgg aggaatcttt aacaccttcc cttacaccgg
    19561 attttcacaa aacgttggtt tggttaaatt gtcaggcatc aaaaaacgcc tgccaatcta
    19621 ctacgcagct ggtttcctgg ttctccttgg actgcttcct aagtttggcg cccttgccca
    19681 aatcattcca agctccgtcc tcggtggtgc catgctggta atgtttggtt ttgtatcaat
    19741 tcaagggatg caaatcctcg cccgtgttga ctttgctaac aatgaacaca acttccttat
    19801 cgcagctgtt tcaatcgctg caggtgtcgg tctcaacaac agtaatctct ttgtcagcat
    19861 gccgacagcc ttccaaatgt tcttctcaaa cggaatcgtc gtagccagcc tactcgctat
    19921 tgtcctcaat gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt
    19981 tttgttgaca aaataaaaac atctacctat tcatatatgc tagtgtcata gtcctgaaaa
    20041 tcatctgcat caagaacaat ttcacaactc ttatactttt ctcttacaag tcgttcggct
    20101 tcatctggat tttcagcctc tatacttact aaacgtgata aagtttctgt aatttctact
    20161 gtatactttt tcagttttag attgctttcc aataattcac ctattaacaa taatctatga
    20221 taggagctat gtagccactc tggcaattta tctcctactt cccacccata tttttccttc
    20281 atcaaagcta gttgtttttc gtcaccaaat ttcatatttt cagataacca tctcacacaa
    20341 tcgggatctg ataaggtaca gacgattaaa cttaggttat cgtaacggct ttcagtttca
    20401 taatcaggcc agatgacttt taagaaatca tacacctctt ctgatgcaac aagttgtttc
    20461 gttttcataa tttccgatac tttcctctac ctttaaattc tattatttct ttgtctctta
    20521 atatttgaag ctgttgccta atcttttctt tgatatgatt gttcttaaca aagatatttt
    20581 ttaggtcact ttcaaaacga tacatatctt caagggtgaa ttctgaaccc tctatcttat
    20641 ctatacaatt tagaatttct attgtccaac ctcttgatga cagagagctc ttccttaaaa
    20701 ataaaccttg cttaaattct tttgtaactt tttctggatc tctaacttgt ccatcttgca
    20761 caagaaatat ccttccttta gaaggtactt gtgataaatc aatgttacaa ccaatccaac
    20821 ctgctcgtct agcagttggt gcaagtggtt ttctttgaat aatcgatttc ggtgtaacaa
    20881 attgcttcgg aaggacaaga aagttattta cctcaaaatt ttttgtgtaa gttaaaaaaa
    20941 agaaattagg attattatct gcctgcacac gcttcatcat cgttgcataa gcaccatcat
    21001 tgattgttga tgaaaaattt ccttttttgc tctttagttc aaactcctca ctacaatgat
    21061 tacagtaaaa atctgctaca ggccgattat tttcaaaatg atttaaggga ttgttcccac
    21121 aattagggca ataagactga cgataaaccc aatcctccgt taagatacgt gccttttggc
    21181 tattactttt ataggtttct actaattcta aattaaagtg taattccatt taaaaattcc
    21241 tcactaatat gagaactcac tcgatataag caattaaaat ctactataac tagtatacca
    21301 catatatgta cctattacat ttgtatttca tcactctctt ttatatttcc ttctaacata
    21361 tctcataaat taaaaatgag tgagataaat ttatcccact cacctttctt tatatactta
    21421 cccgtctttc ctcagacctt tggcctgtga caaacatggt aaaggttgct ttacagacat
    21481 ttctgccctc ttgattggtg atatacacat ccatcacaca ggttgtacga ccttgatgga
    21541 cacattctcc tttaatggtc aatacatcgt cgagttttcc tgccttaagg tagttgatag
    21601 aggattggag tgtcacccca tcaagtccca gcgagataac caccaaacca ctgatttggt
    21661 cacatagcgt gaatagatag ccaccatggg cattgccata gtagttgagc gacgagttca
    21721 ctactttggt cgtcaccaca acgtgaccat ctctcatttt ttcaatttcg taattttcaa
    21781 aggcagatat agcgtcaaaa tgaaagtctt tcatcgtttc ctcctgatat ttcaaacgac
    21841 actatgatac tactttttat aggactgtgc aaggagaaag ctcctagtct agtgaaattc
    21901 cgacctgttc cacaaagcgc ataaaggctg tctgcccttg ttctgtctgc ttgtagactc
    21961 ctgcatcctc aagtacacgc acaaagatag cacccacaga gtccttgacg attgcaaggg
    22021 ctttttcttt atccgttagg tctggatgtt gggatttgag ttggtctgcc cactcctgat
    22081 gataatcggc aactgtaaca gcttctccta caagatagct agcgacttgc tccacttctt
    22141 ctttcagacg tggtggcaag attgccaagc ccatgacctc aatcaagccg atattttcct
    22201 tcttgatatg ttggacatcc ttgtggggat gatagatgcc atcaggatgc tctgctgaag
    22261 tctgattgtc tcgcaagacc aagtccaact caaactgtcc atcacgctta cgcgctattg
    22321 gtgtgatggt gtgatgcggt gtcccgtctg tctctgctaa aatctgcaca ctagaatctg
    22381 aatactgacg ccattcctgg aaaatcttat cagctaagtt aatcaaatcc tctttggaat
    22441 ccgaagtcaa gcgtaggaca gacattggcc acttgataat tccagccttg acctgctcaa
    22501 aaccagcaaa tcggaaggtc ttttgcaagg gagccaattc cataggaaat acgtgacggc
    22561 ctccctgata atgatcgtga gttagaatag agccccccac aatcggcagg tcggcattag
    22621 atccagcaaa atatcctgga aactgctcta cgatagccaa cagacgttca aaactctgac
    22681 gactaatggc catgggacga tgctggccat ctaaaaagat acaatgctca ttaaagtaag
    22741 catagggcga atactggaac ccccattcct gaccaaccat ttcaaaacgg ataatccggt
    22801 ggttgctgcg agctgggtgg ttgacccgac catggtaacc ctcattctct aaacaaagtt
    22861 gacactgagg ataattacta gcttgcacca acttggctac cgcaatctct ttcggatcct
    22921 tttcaggctt agagagattg atggtaattt caagttctcc ataatcagat ggaacacgat
    22981 aagcgatatt tttagcaatg gccttgagtt tgatgtagtc atttttctga ctgagttgat
    23041 aaaaatcctc tatcgcttgc tcaggagagt aggcataggc ttcccagaag tcacgattga
    23101 cctgactcgg acaaggagtc accaagtcca tcagttcagt accaaggatt tcacgcgcag
    23161 tctgactatc ctcaatcgtc tctaatcgaa cggcttcctc aactagctgg tccttgaggt
    23221 caatcaattt atccagattg gtctcaactt ccaaaacacc ttctcccact cgtgccaaaa
    23281 cacgattggt cagatagatt cgatccattt cctcaaatga gctttcagaa atgacatgtg
    23341 ttacaaattt atctactaag gtcactatag agcctccttt tgactagtca aggacacgag
    23401 tgccacctgc aacttcagcg atatagaagc ttggagcgta tccaactact tcctcgtagt
    23461 gtttgcctac agcttcctta aaggcctcaa cagtatcttt ttgaaccaag gcaatggcac
    23521 agccaccaaa accagcccct gtcatacgag caccgagaac tccttcttgt gcccaagctg
    23581 tgtgaacaag ggtatccaat tccaaaccag ttacttcata atcatgctcc agagaaacgt
    23641 gtgacgcatt catcaagcgt ccaaatgttt ccaaatctcc tgcttggagt gctacttgag
    23701 ctttgagggt acgttggttt tcaagcacag catggcgaac acgtttcaaa cgattttcat
    23761 ctttaatcag atagctatat tggtcaacgg cccactcgtc caattcaccc agagtctgaa
    23821 tatccaagga aacttgcaat tcttccactg ctttttcaca ctcagcacga cgttcattgt
    23881 atttagaatc cgccaattca cggcgtttgt tggtgttcat gataacaacg acattgtcct
    23941 tcaaatcaag tggcaccaaa tcatactcta aggtgttggt atcaaggtaa atagcacgtt
    24001 gatcagcccc cataccaata gcaaactggt ccatgatacc agagttgact ccgataaagt
    24061 tattttctgt ttgttttccg attttaacca aatccaaacg ctctaatttt aaatcaaaga
    24121 gatgctctgc cactacccct gtcaagagct ccaaggatgc tgaagaagac aaaccagcac
    24181 catttgggat attcccataa acataaaaat caaaaccttt gtcaatcacg tgcccagctt
    24241 cttgcaagaa atgaaggaca ccttttggat aattggtcca gttgtgctct ttttcaaact
    24301 tgaggtcagc gagaggcact tcgataatgc ccttgtcctc aaagttagct gagtagaaac
    24361 gcaaaacttg gtcgtcacgc ttacgagctg caccgtaagt tcccaaggaa atagtagcag
    24421 gaaaaacgtg cccaccgttg tagtctgtgt gttcaccaat caaattaata cggcctggtg
    24481 aaaagaaagt ttggtctgct tcttgaccaa aaacagcaag aaagtctttg cgaagagttt
    24541 cagcagtaag atgttgtgtc atatgatttc tcctttgact gtccgttaag ccaccttaac
    24601 gtgtaatcgt tttcttctat tgtatccaag gaatttccca aggtcaatcc tttttactaa
    24661 aattttagta aactatcagt aaaaagaaga aaaatatgat atactaacct aatcaaggag
    24721 gatttatggc taccttaaaa gacattgcac agctagcctc tgtctctatc gcgaccgtat
    24781 cccgcgtcct caaccgcgac cagagcctat ctgttacaga agaaaccaga caccgtattt
    24841 taaccgttgc tgaagagctg ggctacacca agcacctcaa gacaggcgag tcccataaac
    24901 ccaagcaaaa gattgccatt attcaatggg tcagcgaaca aggggagctg gacgacctct
    24961 actactacca gattcgccta ggaatagaaa aaagagccca agagttggac tatgatatct
    25021 tgcgctattt taatgaccat ccttttacct taagcgagga agtgattggg attctctgca
    25081 tcggaaagtt tagtcgagct cagatttctg cctttgaaga ataccaaaag cctcttgtat
    25141 ttctagacag cgatacactt tccctgggac atacctgtat tatcacggat ttttacactg
    25201 ctatgaaaca ggttgtcgat tatttcctca gccaaggaat ggaccgtatc gggattctaa
    25261 caggccttga agaaacaact gaccaagaag aaatcattga ggataagcgg ctggaaaatt
    25321 tcagaaacta cagtcaagca aaaggaatct atcatgatga actgatcttt caaggaaact
    25381 ttactgccca gtctggctat gacttgatga aggaagccat tcagaacttg ggagaccaac
    25441 tcccaccagc atttttcgca gccagcgata gtttagccat cggtgccctt cgtgcgctcc
    25501 aagaagctgg aatcagccta ccagaccgtg tcagcctcat ttcctttaac gacaccagcc
    25561 tgaccaagca ggtttatcct cccctctcca gcatcacagt ttatactgaa gaaatggggc
    25621 gagcaggtat ggatattctt aacaaggaag tcctccacgg tcggaaaatc cctagcctga
    25681 ccatgttggg aaccaaactg acattaagag aaagtaccct aaatcaagaa taggataaca
    25741 taaaaaacga ataggagttt tagaactcct attcgttttt tattcgatta taatcataga
    25801 cttaatagtc ttacgttcat ccatatcttt gtaggcttgg tcgatatctt ccagtttata
    25861 acttgaagta aagacgcgac ctggattgat atcaccatca aggacggctt ttagtaaaaa
    25921 ttgcttatcg tatgttgtag cagaagctgc cccacctgct acagagatat tttgcataaa
    25981 tgtcgaacca agagcacgat tattatagtg tgggactcct acaaagccca tacgccctcc
    26041 attatgaaga acacctagcg cctgttctat agcagcctcc gtaccaacac attcaagtgc
    26101 tgcatctgct cctccaccga ggatttcacg caccttggta attccttctt gaccacgttc
    26161 tgcaacaaca gctgtcgcac ctgactccat agccatcttt tgacggtctt catgacggct
    26221 cataaggata atttgtgatg ctccacgcat cttagccgcg atgacagcac attgaccaac
    26281 agccccatca ccgataacaa caaccttgtc ccctttttga acatttgcaa cacgcgccgc
    26341 atgatagcct gtcggcatga catctgcaag agtcaaaagg gacttgagca tcccttctgt
    26401 atagtcagaa ggttgaccag ggattttaac cagcgcccag tttgcatagt ggaagcgaat
    26461 atattctgcc tgaaaatcac cccccaaatt attgccaata tgattgtcgc aagaaccgtc
    26521 aaatccagca agacaggcat cacactcacc acatccatgt gtaaaaggga caatcacaaa
    26581 atcacctggt ttcaccgtcg taatggcttc cccagcttct tcaacaatcc caatcgcttc
    26641 gtgtccacta tttttgtgtc cagctttcgt ttctggatta cggtacctcc ataaatctga
    26701 accacaaacg cacgcacgaa ccacacgaat aatcacatca tccgcttcta ttatttgcgg
    26761 acgttcaatg cttgcaagtc caacctgacc tgcctttgta tatactgctg atttcattta
    26821 aaattttcct tccttataaa gtttaatttt gagatttaaa cgatttaaag ctgtctgtag
    26881 ctgagacaag cgctcctcta atttttgctt ttcctcttct aaaataccaa gcctctcctc
    26941 tctcgtttca tctccctttt ggtagagcga catatagtca actaaactat ctacagagac
    27001 acccgccgaa cgaaaacact taataaattc cagcgcttcg atatcctgat cttgaaaatc
    27061 acgaatccca gtagcagtac gagtaatcgg tggcacaaga ccaacccgtt cataataccg
    27121 aatcgtatcc gctgaaattc ccaacaaatc actggcagat ttaatattca cacgctaacc
    27181 tcctctcatt tcattcactg aattcacgct attctaactc acttatctta ttatataact
    27241 ttgagttgac tccaagtcaa gcattttgta tctaatcgaa aaatttttat cattaacaat
    27301 catatatctg gagtttatac aatgctcttc tcttaccaat ttttgaaata tttaaataat
    27361 aaagaaatta ctctattcca aataagatag tacccaagta tttttcactt ttttctaatg
    27421 ttgatgctca taactccgtt ccaagtcaca cacctttcgc ttatgggttt ggtgagtttc
    27481 taggtcagca tcaatttcaa tggtaatatt ttgaaaacca caatctttta ggaaaattcg
    27541 aatagactct ttacaagttt ccatatgttc catttctttt agacaaacat gtacaatggc
    27601 atttttttcc aaagcatcca tagtccagag attaagctga ttaaggctgg ccacattgtc
    27661 caatcgctcc aggccactct ttacttgctt gatatcaaga ccttctggca cagcatccaa
    27721 gaaaatcttg agtgtagacc aaaaacgtgg aagggctttt gaaagaataa agaaagaaat
    27781 gacaagggac aaaagaggat ctaggatata ccagtccgta aatcgaagaa caatcgccat
    27841 caggataaca gctacccacc ctagcgtatc ttccagaaaa tgcaaactca gaatagactc
    27901 attctttgtc tttcccttac caaccaccag actcgctaac agattgatag taatcgcaat
    27961 aattcctaac cagagaatcc cctcatcatt gactggttgc ggatgcaaaa tcttcgtgac
    28021 attttccaaa atgactagaa cagagcccgt tacgagaatc acagctgtta ccaaggctcc
    28081 tagcaggcta aaccgcttat agcccaaggt gtactgattg tcttcttcac gattggagat
    28141 tgtttctaga aaagctgata ttccaattgc aatcgcatct cccaagtcat gcacagagtc
    28201 agcaagaaca gcgctagaac caaatactcc acctgcaata aactcaacaa tggcataagt
    28261 caaatttaag aaaaaagcca cccaaacagc atattttgcc ttcatatttc tcattccttt
    28321 gttataatag atttatgaac accttgttca ttatcattat ccactaaaac tcaaagaaag
    28381 gatagaagca aaacgtcagc tttattcagt tctgaacaat ttgcctcaag tgtccatatg
    28441 actaacattg accgccgtat cagcaaaacc aaaaaagcca tctatcaagc ttttatacaa
    28501 cttttgaatg ctaaggacta cgagaccact actgttcagg atatcattga tctcgcagat
    28561 gtgggacgat ccacctttta ctgtcactat gagagtaaag agcttcttct ggatcagctc
    28621 tgccgctacc tcttccatca tctttttgaa agagagcaag ccatttcaac cgaggactac
    28681 ctcgcccacc tctttctcca ttttcataaa aaccaagacc atatcaccag tctgctattt
    28741 tccaaaaatg actacttcct ccgtcaactc cagagagagc ttgagcacca cgtctattcc
    28801 gtgctagctg ataatttaaa agaagcccac cctaatctgc ctacttctta cctccaacac
    28861 ttggtcatgt ccaactttat cgagacattg acctggtggc tcaaaaaagg tcaagatttc
    28921 acagaccagg aagttgtcca attttatcta gaccttctca ttcctaaaaa ttgaatatag
    28981 agtaaagctc agttgtctta tttctaggtt actgagtttt ttatcttttc aacaacaaaa
    29041 gaggacccgc cgatcctctt tttcatacta taaatccttg attatcaact atatctgttt
    29101 taatcgaaat ctcaaaacag cactttcaaa catcttttcc tagttaagta aatcagtatt
    29161 ctgcttagct gccttgctcc attgatacca accaactaga ctgttaatga gataaattag
    29221 atatttccct tgaatttgca ggctttctcc ccaccagaga tagattgaaa aaacattggt
    29281 agccgcccag aatatccact gttcacggta aacagctgtc atgaggattt gccctacccc
    29341 attggttgca tctgtgattg aatcacgata gggacgattg gcaccaatag actgataaat
    29401 gaagccaaag gccaaccacc aaagcacact aatggaaaga tactttgtcc agcccttgcc
    29461 gtccagttta cgcgcgacaa actcctgctt ttccttctta aactgtgcct gataaatcca
    29521 aactagaagt ccaattggct gcatgactgt gaagtaaagt gtcgtcagca cctcaccata
    29581 aaagcctttc tgtagggcca aaataaggta aataacagag ttaatcaagc caaaaagata
    29641 attacttgct cgaccttccg atacaaagat tacacagata atccctgtca agctacaaat
    29701 catcccaatc cagtcaacaa tacgatgttc gtaaaccaac tccagccaga gaggaaaact
    29761 tcctaaaacc agcaaataaa tccactgggc aaaactacga tgggcaaaga ggtcatccca
    29821 gatagccttc atagttcctg aaaatcctaa atcagccata gccgcaacca tacgacggta
    29881 accacctgac atttcaccta gggttgtttt gatattttca attttctttt gcaaataagt
    29941 atgcatcatt tctccttttg tttttaaaga gccgtgtctg gatagacttt cggacgcaac
    30001 gctctattag ataatgaact gcctatacac aagatttcta accttagtcg acatgagctg
    30061 aaacctctta tttgttaagt agttcacaaa atattataca cctattttat gaatagtcaa
    30121 ctgtctttac agtaaaattt tagaaaatca tgaaaatttt ctctttcttt ccattttaag
    30181 tgacattcag tcattctcac atcaaaaaag cccagacgaa attgtctgag cattctttta
    30241 tctagtcgtt taaggaagtt gagttcagta tgtttaaagt ctctgtccca tcatttcttc
    30301 aacaaacctt gttcttggag aaactccttg gctacttgct ttgctgactt gccttcaaca
    30361 ccgacttggt agttgagctg gctcatctgg ctttctgtaa tcttaccagc caatgtatta
    30421 agaactcttt ccaactctgg gtgtttcttg agaagagctt ctttcatgag tggagcccct
    30481 tgataaggtg ggaagagttg cttgtcatct tccaagacct gtaaatcata acgctccaat
    30541 tccgcatcag tcgaataggc atccgtgatt tgaatatccc ctgactgaat tgcctgataa
    30601 cgaagagctg gctgcatagt cgccacattg agattgagac catacattga ttgcaagccc
    30661 ttatttccat cttcacggtc gttaaactca agcgtaaagc ctgccttcag ctgcccttcc
    30721 acttttttca aatccgaaat ggtcttcaag ccatattctt gagcaatctt tttcggaaca
    30781 gctacagcat aggtattttg ataagacata ggtttgagat aggctagatg atcctgcttg
    30841 gcaatgccat cacgcgccac ttgataaacc tgctctggct catgactcac cttgggtgat
    30901 ggttgaagca aactttcagt caccgtaccg gtaaattcag gataaatgtc aatatcacct
    30961 tttttcagag cttcataaag gaaacttgtc gtcccaaaat tcggtttaac agtcgcagtc
    31021 atgctggtat tttcttcaat cagcaattta tacatattag ccaaaatttc tggttctggg
    31081 cctattttcc cagcaataat caagttttcc ttctcttttt gagccaaaag ggctggacta
    31141 taagacagac ccagtaataa agccatcaag gcaaaaccag agaaaatcgt ccgcaatttc
    31201 gctttttcca ttacttttag taggaagtta aaggcaatag ctagcactgc agaagaaagg
    31261 gccccaatca aaatcaaact ggcattatta cggtcaattc ccaacaggat aaaggaaccc
    31321 agtccccctg ccccaatcaa ggtcgccaag gtagccgtac cgataatcaa gaccgctgcc
    31381 gtccgaattc cagacatgat aacaggcatg gcaagtggaa tttcaaactt cttgagacgc
    31441 tcccatctgg tcatcccaaa ggcaatccca gcctcttgca ggttcggatc aattcccttc
    31501 agcccagtaa tagtattttg taaaatcgga aaaatcgcat aaatcactag agctgtcaat
    31561 gccggtaaag tcccaattcc catcaaggga ataaagagtc ccaacaaggc cagagacggg
    31621 atggtctgga aaatccctgc aatctgtaag acccaatccg ccaacttctc atgatagcga
    31681 agataaacag ccaagggaat cgcaagcaaa atagctagta acaaggtcaa aagtgacaac
    31741 tgcaaatgtt gagatagggc tgtcaaccaa tcaccaaaac gatcctgaaa agttgcaatt
    31801 aaattagtca tgaatactac ctccaaacaa gtctgcaaca aagtctgttg caggagcttt
    31861 taaaatagtc tcaggattcg acacctgacg aatctctcca tcctgcaaaa cagcaatacg
    31921 atctgccaac tttaaggctt catccgtatc atgagtcaca aaaatcgttg tcatcccaaa
    31981 ctctttatgt aattcttttg tcagaacctg caactgcttg cgagaaatag catccaaggc
    32041 tgaaaagggt tcatccatga ggagaatctt gggctgacca atcatagctc ggacaatacc
    32101 gacccgttgc tgttctccac cagatagctc acttggaagt cgatgcccat actcagctac
    32161 tggtaatcca acttttgcca aaagctcttc tgttttttga gcaatttctt ccttagtcca
    32221 acccttcatt tcaggaatga gagcaatatt ttcctcaact gttagattgg gaaagagagc
    32281 aatagcctgt aaaacatatc cagtagtaag acgaagttca cgctcatcat agtctttgat
    32341 gcgcttgcca tccatataaa tatttccatc agttggttcc aagagacggt taatcatctt
    32401 aatcatggtc gtcttacctg acccagaagg cccaactaaa accatgaact ccccattctc
    32461 aatctgtaaa ttgacatctc tcaagacatc tttttctgtg tagcgcagtg ctacattttt
    32521 gtattcaatc attctttgtc ctcaatttaa aacttccctc gattagtcaa gtcttctacc
    32581 ttaggtatga cttccttatt atcccaatgc tccacaattt tcccgttctc taaacggaag
    32641 atatcgtact gggcataagc aacgccatca atctgagtct gaccatagct aaccacatag
    32701 tttccttgtc ctaagagttg gaaaacaaag tcaaaagtga cactatattc agccacatag
    32761 tttttataag cagcacttcc ttgtccaatc tcatgattat gctgaatcaa atcgtctgcc
    32821 acataatcac tccactgctc tagctcccca ttttggaaag tttctgtcaa gaaacggcga
    32881 accagctttt tattttctgc tttcttgtcc aaatccttga tttcaaaatc tccaaaaatt
    32941 tgatctagtt ggtcattttc aggtgttcga tagtagtcaa tgacatccca atgctcaaca
    33001 atacgaccat tctcatccgc acggaaagta tccgtcgtca cccattgagc ttctccacca
    33061 ttcagatatt gatgaacatg aacaaagacc agattgccat cctcaatggt gcggacaatc
    33121 ttaatctgac gctctggatg acgctcaaag aaatctgcaa agaaggctgc aaatccttct
    33181 ttcccgtcag gaacacctgt cgaatgttgg atataggtat cccctacaga ctgggcttga
    33241 gcctcagcaa ctcgtccgtc ttgaatggca tggatgtata ggttgtgagc atttttcact
    33301 tgttgtgaca tattctaaac ctcattttcc ttctctttca gatttgccaa aattctttct
    33361 tgaaaacctt caaattggtg aatttcttcc tctgaaaatc ctttgtaaaa gatagtatcc
    33421 aatttctgac tgacacgatg ccccacttct ttctggaact tgcctaactc cgttaaaact
    33481 aaatacttct tacgcttgtc ttttccacac ggactaacaa ttacaagctt ttgttcctct
    33541 agctttttta tcatagtcgt cagcgtatta ttcgcaagcc cagtcgcaag cgcgatatct
    33601 gtcgcagttg cgcagccagt ttcactattc cataaaaccg ctaaaatctt gccctgttca
    33661 cccctataaa gagcctcagg atcttgactc agtaactttt gaaaaatccg cccattcaac
    33721 aaacgaatat gatgggctag caaatgacta tctttcataa cacctccaat ttatttcgat
    33781 atcgaaatga ataaaataat tgtaacactc atcgttctaa ctgtcaacta tttcgatttg
    33841 gaaataattt ttgataatta ttcacaccac catactccgg ctcaactaac ttttaacgag
    33901 agtttctaaa ttccttcgtc ctccagtcta caaaagcctt ccatccgtac tatcctattt
    33961 tttatgaggg gacacatttt tcctatcaga tcatttattt taaagataga agtaaatcat
    34021 aattgcttcc atctgttctt ttatagtata ttgaagttgg actagggcac tgtatcttct
    34081 aaaacattga tagaaagcga tttgaatttc ccaatcaatt tgttcgtatt tatagcattt
    34141 tgaaactaga atacgacgcc atgactgcta aaagatttct ataaattcat ttgatttccc
    34201 taatcaattt gttcatatct tatttcattc cgctataatt tcaccttacc ctatcttttt
    34261 cgtagcaccc ttcaaacagc ctatccccta ccgtttgacg attcctcact tcactccact
    34321 tccattacag aagtttcttc actactatgg tctcggctga cttctcatga ttccttgtta
    34381 ctactatttg aacgctcacg agataggtct tataaaaaat gctttgatcc acaatggaat
    34441 caaagcattt taaaaagttc ctcatacata agcgcagaag tcgcagttcc tctgtacttg
    34501 gcttcttctc ttttgacaaa gcgagccaag ttgagcaact caggtgctgg atgtttggga
    34561 tttaggagca attcacgatt gaccaggctt gagagacgaa ctgcctgcaa ttgctcattt
    34621 gtagtaggca gttttttagt agtctctagg agagcagcaa ctaaatcttc actcaaatca
    34681 tgtcgagcat gattgtaaag atcttttata aggctttcta ggtttggttc taccatccct
    34741 accacctccc ttatggttta ataatgttta atcaaatcaa ccgttgaacg atccaatttc
    34801 ttcaccaagg cttgtaagaa agcttgcgct tctaggaagt catccattgc atagagggtt
    34861 tggtgagaat ggatataacg agcgcagaca ccgatagttg ttgatgggac accaccattt
    34921 ttcagatgag ctgcacctgc atctgttccg cctttaccac agtagtattg gtacttgata
    34981 ccagcttctt cagccgttgt caaaaggaaa tccttcatcc ctgggagaag caagtgacct
    35041 ggatcataga aacgaatcaa ggttccatct ccaatcttgc cttgaccacc gtagacatca
    35101 cctgctggtg agcaatcaac tgcgaggaag acttctgggt caaacttggt tgtagaggta
    35161 tgagcgccac gcagaccaac ttcttcttgg acgttagaac ccagatagag ttcattgccg
    35221 agtttttgac ctgataaagc ttcagctagc tcgcttacca tgaggacacc gtagcggtta
    35281 tcccaagctt ttgagatgat atttttttca ttggctgtca aaattgcaga actatctggt
    35341 acgatggtat caccaggacg gatgccaaaa ctttctgcct cagccttgtc cgcaaaacca
    35401 ccatcaaaaa cgatatcggc aatggctggc atggttggtc ccccctttcc acgagtcaaa
    35461 tgcggaggaa cagaacctga aatcacagga atttcatgac catcacgagt caagagtttg
    35521 aaacgttggc tgctaaccac catggggttc cagccaccga tttctacgac acggaaggta
    35581 ccatctggct tgatttcgct gaccataaaa ccaacttcgt ccatatgaga agcgaccaag
    35641 acgcgcggtg catccacagc ttctgaatgt ttgataccaa aaataccacc caagccatct
    35701 gtcaccactt catccacatg cggtgtcaac ttttcacgaa gataagcacg gacaggcgct
    35761 tcatgacctg agactgcagc aagttctgtt acttctttaa tttttgaaaa taatgttgtc
    35821 atttcagttc cttctttctt tcatccattt taccactttt tataggagaa ggatagtggg
    35881 aaggtggatt tctaagttag tatcttagtc ctgctctatc ttagaaaagg atagtattct
    35941 cttgcatgta gtgcaaaatc tagtaaacat tccaaaatta actcgaatat ttatttccaa
    36001 acaaaaaaac aatacaccat caaagttgtt tggatttttc atgaaattta cagaaaatag
    36061 ttgacttccc tttcttcttt ctttaaatat atagttggtt gagtttggaa tagtacgctg
    36121 tagctgctga aacatttcta gaaattaatt tgactttcct aatagagttg ttcatatctt
    36181 atttcaattt actatagtac aaaactagaa aaggaaaaaa tcatgaccaa aacaattctt
    36241 gttacaggtg ggactagcta cattggctcc cataccgtta aagctctttt aaatgctggc
    36301 tatcaggtgc atgtcctcga taatctctcc acaggaaatc gagcggctgt ggatagtcgt
    36361 gctagcttta aagaactgga tgtttatgat gctagcgcct taaaggctta cttagaagaa
    36421 aatcagattg atgctgtcct ccattgtgca ggtgaaattg ttgtgagcga aagtattgaa
    36481 aatccaagta aatacttcac tgccaacgtt gctggtatga accaagttct caaagtctta
    36541 tctgaagttg gcattcaaaa aatcatgttc tcttcgactg cttctctcta tggaaataac
    36601 tgtattgaca agccggtgac tgaagatacc ctgctcgacc ctgtcaaccc ttatgcagag
    36661 acaaaactga tgggcgaacg aatgatttac tggatggcca atcgctacga ctggaaatat
    36721 gttattttcc gttactttaa tgttgctggg gctgaaatgg atgcttcaaa cggtctgcgt
    36781 gtgaaaaatc caactcacat cattccaaat atcaacaaga ccgcattggg acaacatgat
    36841 agcttaaaaa tatttggaga tgactacgat acacgtgacg gttcatgtat tcgagattac
    36901 atttatgtct tggaccttgc ataggctcat gttaaaggaa tgaactacct atttcaagaa
    36961 gacagttctt ctcaaatctt taacttagga actaaaaaag gctatactat caaagaaatc
    37021 tttaaaactg ctgaagaatt actaaattaa aaaatcccac acgagattgt tgctcgacgt
    37081 gctagtgacc cagccagtgt cctagcagat gcttcaaaag ctaaacaata tcttgattgg
    37141 acggctagct actccctcga agatattatt ttatcagatt aaggtaaaaa cattttagaa
    37201 taggataaca aaggagaaag tcttgttgct ctctccttgt tgtatttatt aaattgtcag
    37261 aaattttatt gacagattag aaaaataatg ttacatttat atccgcagat atctttcgat
    37321 accaaatcta catgaaagga cggggtatga aactttctca ttatttaatt ggcttacttc
    37381 tactcctagt ctttctctct attagcattg ggaccagtga tttttcatgg ggaaagctat
    37441 ttgatttcga ccagcagacc tggctcctct ttcaagagtc ccgtctccca agaactatca
    37501 gtattctcct gactgcctct agtatgagta tggcaggcct tctcatgcag actattaccc
    37561 aaaatcagtt tgctgcaccg agtacagttg gaacgactga agccgccaaa ctgggaatgg
    37621 tgctgagcct ttttgtcttt ccatcggcta gtctgaccca aaagatgctc ttcgcttttg
    37681 tttcatccat cgtattcacc ctcttcttcc tagcctttat gaccattttt actgtaaagg
    37741 aaaggtggat gttgcctctg attgggatca tctatagcgg gattatcggc tcagtcacag
    37801 aagttatcgc ctatcgtttc aatctggttc agagtatgac tgcctggacc cagggctcct
    37861 tctccatgat tcagacccat cagtatgagt ggctcttctt aggcctcatc atcctgataa
    37921 ccgtttggaa attatcccaa accttcacca tcatgaatct agggaaagaa accagcgaga
    37981 gtttggggat ttcctactcc ctacttgaaa aactggccct ctttctggtg gcgctaacga
    38041 caagcgtcac catgattacc gtgggtggcc taccatttct cggagttatc gttcccaatc
    38101 ttgttcgcaa gcgctatgga gataatctaa gtcaaaccaa actcatggtc gcactggttg
    38161 gtgccaatct agttctggct tgcgatatcc tatcccgagt tctgattagg ccctatgagt
    38221 tgtctgtcag tctcttgcta ggaatcatcg gtagtctcgt ctttatccta cttctctgga
    38281 gagggggacg aaaagatgca gactaaaagc aaacatacca agctcttctg gattctcatt
    38341 attcttgcca tcggagcttg tcttctctac ttttggccca tcactcactt gtcagccttt
    38401 gcttggaagt tgcgttccca aaagatcatc gtttatctct tggtagccat cgcgactggg
    38461 atttcgacca ttagttttca aaccctgacg gaaaatcgct tcctgacgcc tagtattttg
    38521 ggaattgaat ccttctacat cctactacaa accctactac tggtttttga aagcaagttt
    38581 cttcaacttg gcaaatcccc tatcttagaa ttcctagtct tacttcttgt ccagtccctc
    38641 ttctttctcg ccttacaagg ttacttgaag acactgatga agcaagacct ggtcttcatc
    38701 ctgctgatct gtctagcgct cagaagtctc tttcgaaata tcagcacctt ccttcaagtc
    38761 ctaatggatc caaacgaata cgataaactg caaaatagtc tttttgcctc ctttcaacat
    38821 ctcaacactt ccatcctagc catcggttct ctgatcatcc tcgctttgac aatctttttc
    38881 tttcgaaaag cagtcgttct agatgtcttg cacctgcaaa gagaaacggc tcagatattg
    38941 ggactcgatg ttgaaaaaga acagaaagag ctcctctggg gaatcgtgct tttgacctca
    39001 acggccactg ccttggtagg acctatggcc ttcttcggct ttatgctggc caacctcacc
    39061 tacctgattg tcaaagacta tcagcacaag ttactcttta tagtggccat tctgattgga
    39121 tttattagct taaccttggg gcaagccttg attgaacgag tctttgcact ggaaattcgt
    39181 atcagtatga tcattgagag tgtgggtggc ttcttattct ttatcttact atataggagg
    39241 tctcgtcagt gaaactggaa aacattgaca aatccattca aaaacaggat attttgcaag
    39301 gcatttcgct tgaagtcagt cctcaaaaac tgacagcctt tattggtcca aatggtgctg
    39361 gaaaatcgac tctcctctcc atcatgagca gactaaccaa gaaagatcag ggagttctca
    39421 gtatcaaagg acgtgaaatc gagagctgga attcgcaaga actggcccaa gaactaacca
    39481 tcctaaaaca gaaaatcaat taccaagcca aattgactgt tgaagaactg gtcagttttg
    39541 gacgttttcc ctacagccga ggtcgactta gatcagaaga ctgggaaaaa atccgagaaa
    39601 ctctgaacta tttggaactg accaacttaa aagaccgcta catcaatagc ctgtcagggg
    39661 ggcaactcca gcgcgtcttt atcgctatgg tactggccca ggatacggac tttatcttgc
    39721 tggacgaacc actcaacaat ctcgatatca agcaaagcgt cagcatgatg cagattcttc
    39781 gacgactggt ggaggaactc ggcaagacca ttatcatcgt cctccacgat atcaacatgg
    39841 ccagtcagta tgcagatgaa attgtcgcct tcaaggacgg ccaggtcttt agcaagggaa
    39901 gaaccgatca aatcatgcag gctgacctac tcagtcaact ttatgagatt cccatcacgc
    39961 tagctgatat caatgacaaa aagatctgta tctatagcta gtaacataaa agctcaagtt
    40021 agagaacctt cagtctctta gtcaataaga ttaagagact ccctaaatcg ttatcacatt
    40081 ttaaaaagga gaaattatga aaacatccct taaactttat ttcactgccc tagtggccag
    40141 cttcttgctc ctacttggtg catgtagtac aaactcaagc actagtcaga cagagaccag
    40201 tagctctgct ccaacagaga taaccattaa aagttcactg gacgaggtca aactttccaa
    40261 agttcctgaa aagattgtga cctttgacct cggcgctgcg gatactattc gcgctttagg
    40321 atttgaaaaa aatatcgtcg gaatgcctac aaaaactgtt ccgacttatc taaaagacct
    40381 agtgggaact gtcaaaaatg ttggttctat gaaagaacct gatttagaag ctatcgccgc
    40441 ccttgagcct gatttgatta tcgcttcacc acgtacacaa aaattcgtag acaaattcaa
    40501 agaaatcgcc ccaaccgttc tcttccaagc aaggatgact actggacttc taccaaggct
    40561 aatatcgaat ccttagcaag tgccttcggc gaaactagta cacaaaaagc caaggaagaa
    40621 ttggccaagc tagacaagag catccaagaa gtcgctacta aaaatgaaag ctctgacaaa
    40681 aaagcccttg cgatcctcct taatgaagga aaaatggcag cctttggtgc caaatctcgt
    40741 ttctctttct tgtaccaaac cttgaaattc aaaccaactg atacaaaatt tgaagactca
    40801 cgccacggac aagaagtcag ctttgaaagt gtcaaagaaa tcaaccctga catcctcttt
    40861 gtcatcaacc gtacccttgc catcggtggg gacaactcta gcaacgacgg tgtcctagaa
    40921 aatgccctta tcgctgaaac acctgctgct aaaaatggta agattatcca actaacacca
    40981 gacctctggt atctaagcgg aggcggactt gaatcaacaa aactcatgat tgaagacata
    41041 caaaaagctt tgaaataagc cacttcaaac tcaatttaat actcaatgaa aatcaaagag
    41101 caaactagga aactagccgc aggttgctca aaacactgtt ttgaggttgc agatgaaagc
    41161 tgacgcggtt tgaagggatt ttcgaagagt ataaaatttt gacattctaa aacgcatcct
    41221 atcaagttca ctcaaacctg ataggatgcg tttttatcat tttgaaatct atttacccca
    41281 cctcattccc cttcttgatt tcgtttaagg gaaaagtggt ctggaacggg gtccttacct
    41341 gttttcgacc agggatgaca acgtaaaatc cgagccaagc ccatcaatac tcccttaaaa
    41401 ccatgttttt caatagcctg aatcatgtag ttggaacaag tcagatcaaa gcgacaagag
    41461 ggtggaaaga ctggtgagat aaaacgttgg taaaagcgca caggcgctat taaaattcgt
    41521 ttcattattt cttagttaca gccatggtgt gtagttggct gatttctttt ttattaagac
    41581 gacgggattc tcctggacgg agtcctgtca agtctaggtg tccgaaacga gtccgagaca
    41641 acttatctac ttggagacca acagcttcaa acatcttttt aacctgatgg ttacgccctt
    41701 catggatggt caactgcacc acagagcgat ttttgactgg gtccactttg agaatttcat
    41761 aaacagctgg cttggttttc ttaccatcaa tctcaagacc acgggtcaag gggcggagat
    41821 tgtccttatt ggccacacct ttaacacgcg cgacataaac cttgtcaatc tcattacgag
    41881 ggtgaatcat ctcgtctgta aagtccccat cattggtcaa aatcaagaca cctgatgtat
    41941 cccagtccaa acgacccaca gggtaaatac gctctttgac attgggcaag aggtcgacaa
    42001 ccgtcttgcg acccttatca tctgtcacac tggaaatcac accgcgtggt ttgttaagca
    42061 gatagtagac cttttcttcg ttgtagatag gttgaccttc aacttcgacc ttgtcgcctg
    42121 acttgatagt ggttgctagt tcacgcacca cttggccgtt aaccgtcacc aagccttgct
    42181 taatcagctc ttctgctttt ctcctactgg ccacacctgc gtgggcaata tacttattga
    42241 ttctcatctt cttctatcct ttcaccaaat aattggcttt cttgggcttg aatctcaagc
    42301 tcatcaatca ctggtaattc ttctaaatgg tttatcccca tgtaatctag gaaataatcc
    42361 gtagtcacat agaggttggg gcgccccaat acttcctttt tcccgtcttc ctttatcagg
    42421 tcaaaagcct gcaactttgc caaggctcca ctcgagttaa ctccacggat ggcatcaatt
    42481 tctatccgcg taatcggctg tttgtaggca ataatggaca aggtctcaag ggcagcccga
    42541 gacaagctct ggttgatagg cgccttagag tattccttca aaatctctgc aaattgaggc
    42601 ttggtcacca atctataagc accacttgtc tcaatcaaag ccaaactgga atctgggtcc
    42661 ttttcatact tctgggctaa ttttcctaaa ctttgctgga tgcctgtcgg tggcagagag
    42721 aggagttcag ctaactggcg gacccgaatc ccatcttcac ccgctacaaa caagagcgct
    42781 tctatttttg ctaaagtact catctttcct ctctatcaag tctagctttg aggcacttga
    42841 ctttcttcct tcttttccat gagatagata tctccaaaac tctcctcttg cacgaggatc
    42901 agctcctggg ttttgattaa ctctagggtt gccaaaaaga gggtgatgac ctcttggaca
    42961 ttctgggctt ccttgaacaa atcctgcaag cgcaattgat ctcgtccaat caaggactct
    43021 ttcacgataa tcatcatgtc ctcaatctta tactcatccc gcaagatcgt cgtgtgattt
    43081 tgtgcaaact cctctttttt cttggctagg atatttgaaa aagccaaaaa gaggtcaatg
    43141 gtcgtcttgt catgcacaag ctccgcatct tcgtaaatca actctgtcgg cgctttggaa
    43201 taatactggg cccgttcttg gtgcttggct tccaagtgct cacccaagag cttgaacttg
    43261 cgatattctt cgatttgaga gaggaggtcc tgctccaggt catcccccaa gtctgtcact
    43321 tctgctacct tcggaaggag tttacgactc ttaatcagca tgagctgact agccatgacc
    43381 atgtactcac ccgtcacttc cagacgcatg gcctgcaggg ttgagacata ggctagatac
    43441 tgttcgatga cttccgtaat gggcacatcg tagatatcca tctggtactt agaaaccaga
    43501 tgcaagagca agtccagggg tccttcaaaa tcttttaatt taatatccat tatctatatt
    43561 tttctaaggt caggactgtt tttaatccta atttttttgc aatttcgtac aaatcgacct
    43621 tgttttctat ttgtcttaga ataaactgtt cacgtaagac ttgagctgat aaggatggaa
    43681 aacctttctc cttgacaaaa gattctaact gacgaaaggc ccactgacga gaatagggtt
    43741 tctctcctct ttcaaaaaga taggtctgcc ccatcaaggg ttccaattct gaaagcaagg
    43801 ccgtgggaat ggtgacaatc ctctgttggg aagccttgct gattcgcagc acctgaaaat
    43861 ccagattgat gtccgcaacc ttgatggcta aaatctcact gggcaagagc cccatttcta
    43921 ggattaagag cgctagcaag cggccctctg gatggtcgct ttcctgccaa aaagagtcta
    43981 ggtatagaat ctctggcttt tccgtcttct tttcagcttg tttggctaat tccaagcggt
    44041 aaaagctgtc cacctctcct ttttgataga gaaagtatag aaattggtta caggccgaaa
    44101 tctttcgctt ctgggcgctg atttttagat tggctagctg ggcttggtaa atcttgagac
    44161 tggtctcaga aatccgctca cctaccatgt ctaaaaattg ctccaaatca tacttatagg
    44221 actgcttgga attgacagac aagccctgct tttcctctaa aaaggctgaa atcctgtctc
    44281 tcattggcat cgaatctcat attccttact aaagtcatgc aagagggcat taacggcctt
    44341 gaggatggac ttgcgcgtaa taatcccttg gaaaataccc tctgcatcca cgaccggtaa
    44401 gaaggactca tctactagct tgtgcaagac ctccgtaatg gtgaaatcag gcgaaacaac
    44461 cgctacgtcc gtttttgtca tatgaacgat atccgtatcc gccatgattt cttggctcaa
    44521 gtcatgctcc atctgataag ccataatatc tctgagtcca atcgtcccaa caaactgttt
    44581 ttcatctgtc acaacgggaa cacgggtata ggtcatctga ctgagcaaga gggtcgcatg
    44641 atccgcattg tgggtatcaa tcaacacagc tagattttta gcaggggtca aaaaagtttc
    44701 ctcctgcccc aacaagaaag tctcaaactc cttggcaatc atcggctaaa ctccttggac
    44761 aaacctggat acacctcgtg atctcgtgtc aaaaagtcca ctttgaagta actatcatca
    44821 atctccacac gagcatagag acattctctg atggtacctc gtggttgact gatagaacct
    44881 ggatttagaa agaggatctt gccttccaac caagcacttg gcacatgcaa gtgaccatag
    44941 aggcagatag cggcctcttc ctcctgagcc cagtagtcca acttttgaaa gttgaaattg
    45001 atgtcaaaca agtgaccatg agtttggata atcttggtcg aaccaagctc agtcaccaga
    45061 cgttctgggt agccggcgta gaagtccatg ttccctttaa caacgcggat gccctcccaa
    45121 agtggagaat ccggacgtag ttcagaatcg ccgttatgaa aaacggcatc gactttgccc
    45181 acatagcgat cacggacttc ttccacaatc aagctatcgc catgggaatc gctcattaca
    45241 atgatggttt gctttgccat gatggaaata cctccaaaag tttcttaacg gctaaggcac
    45301 ggtgagattg actatttttt tcttctaggg ttaattcagc tgatgactca cctgtctctc
    45361 ctacaaggaa gaggggatca tagccaaagc cattttcacc cttaggttca aagttaatat
    45421 aacctgacca gtctgcttca acaactaaac tttccttatt tgggctggct acgactaggg
    45481 ttgtgtggaa ctgagccgag cggtccttga gttcaaagac catggccaat tcgtgcaaga
    45541 gtttggcatt attttcacgg tcagttgctc ccacacctgc gaaacgagct gaccagacgc
    45601 ctggtaagcc accaaggaca tcgactttga gaccagaatc atctgccaaa accatcttac
    45661 ccgttaattg agaaatggtt tctgccttaa ggcgggcatt ttcttcaaag gtcatacctg
    45721 tttctgctac ttcaggcagg tcagggtagt cattaagatt ttccacatcg tagcctaact
    45781 tatcaaagat agctcggaat tccttggttt tgccttcgtt acgagtcgca atcaacaagg
    45841 tttctctgac cttgcttgtc tcaaagaaat catgaacatt gaccccttct ttaggcaatt
    45901 ctacatacaa gagttgccca ttttcaacca agagcagggc cccatgacgt tgaataactt
    45961 ccaagtttcg tcccatttct tgattaagca tatctaccac aaaggagaac aaacggtagg
    46021 tagaaccata gcgtaaaaca gtaactgaaa gcgggaaacc atactcttca tctccaaata
    46081 ttttggagaa ttcaaacaga ggaaaatctg tcttatagtc gctcaaactg ttaacgccac
    46141 caaaaatact ataagaccca acataccagt cctggtcatc cttatattca taaattttat
    46201 ttgtcataat tctacatgct ccacatgaat ctctttttcc agccattctt caccaatttg
    46261 tgcaaaactt tggctactgg ctgttgtgta aaaacggtga tggagtggtc cagcatcgcg
    46321 accacgattg atttcaaaat aattgagtaa gactgagata tcccgtacgc actctgcccc
    46381 actatcgatg agctgaacct ttggccccat cacattttgg ataatagggc gaaggagtgg
    46441 ataatgagta cagcccaaaa tcaggctatc cacctttcca accaagggac gcagggtttc
    46501 atagaccacc ttcttggtaa cactggttga cagggcacct gactcaacca agggagcaaa
    46561 cttgggacag gccaagctct ccacctgtaa gtcgggatcc agatcatgga ttttctgacg
    46621 gtatatgtct gattgtaccg tcatgggcgt tccaatcact ccgattttcc caccttgact
    46681 ggacttgatg gctgccgaag ctcctggcaa aattacaccc aagacaggaa tatctagttg
    46741 agccttgatt tcttcccaga cgaccgcagt cgcagtgtta caagcaatga caatcatttt
    46801 gacatccttg gtcaagagaa agttgaccag ctgccaagta tattcacgaa tttgctcagc
    46861 aggacggggg ccatagggcg cccgcgccga atctccaata tagacgattt cttcatgggg
    46921 aagctggcgc atgagctcgc gcacaacggt caagcccccg acacccgaat ccaaaaaacc
    46981 aattggtcga ttatccatac agtcttcttt ctagtctttt ttctgattga tcgttcatta
    47041 tgattccttg tcctgaacga actgaccgac agcttgatag aatgtcaact acctctctct
    47101 taatcataat caaatatcaa tagaatgcaa ggaaaaagtc tcatccctaa accataacca
    47161 acatagtgag aagtctggaa ctttcatccc agacttttcc ttatttattt tttcttttta
    47221 ttgttagcaa gggctgcctt ttgttggcgg atgatttggt ggtaaacttg ttgtacctta
    47281 gcttcgcttg gtttttgacc atttgcactc aaaagagtac gaactgcttc agcattcaaa
    47341 cgtgggttgt cagcaaattc tttttcgatt tgcttacgaa ccaagtacat tcctccaaga
    47401 gctcctccta gaaaagctag cacaatcaat acaattgcta aaagtaaatc catcattttt
    47461 ctcctgtttc tttttgagtc cctttcatta taccacaaac tagccaccct gaccagtttg
    47521 ttctacactt tcagggatgg aatagacagc aaaaagaacc ctgcttttct gaaaaagagg
    47581 tctccttgct aagtttatac tcaatgaaaa tcaaagagca aactaggaag ctagccacag
    47641 gttgctcaaa gcaccgcttt gaggttgcag ataaaactga cacggtttga agagatttgc
    47701 gaagagtatt aatttacata aatagccagt gtttgatagg gtttgagtag aattttctca
    47761 gacacttctg catcttcata gtttgatatc aaaatctgtc cattttggta gactgctggc
    47821 aagtcgattt ccacttcttt agcataaaag ttattgagca ctagtaactt ttgatcctca
    47881 aactggcgtt caaaagcgta gacttgtttg ctatcttcaa aggctggttt gtaacttcct
    47941 tctgaaatga ttggcatttc cttacgcagt cgaatcaagt cttgatagaa ggtaaaaatc
    48001 ggaccctgga tttcattttc tacattgatg tctttatagg atttaccagc tttcaaccaa
    48061 ggagtgcctg ttgaaaatcc tgcattttcc gaagcatccc actgcatggg aatgcgtgaa
    48121 ttatcacgcg acttagcttg aataatctgg aaggcttctt gctgactctt tccttcttct
    48181 aagagcatct gataggcatt aagcgattcg acatccacat aatcagccat agaatcatag
    48241 tctgggtcaa tcatcccgat ttcctcaccc atgtagatat aaggtgtccc acgtgacagg
    48301 tgaatgctgg ccgctagcat ggtggctcct tccttgcgga agttttgaat atcgacaaaa
    48361 cggttcaagg cacgtggttg atcgtgattg ttccaaaaga gggcactcca accgtcttta
    48421 tcactcattt ccttacccca actatggtaa agactcttca actcttcaaa atcaaaggga
    48481 gccaaggtcc acttttgtcc atccttatag tccaccttga ggtgatgaaa attaaaggtc
    48541 atggataatt cctgacgatc aggcgacgaa taaaggacac agttttccat ggtggtagaa
    48601 gacatttccc caactgtcat aaagctatcg tcagatccaa aagtggcttg gttcatcata
    48661 cgcaaatagt tatgaacgat gggtttgtct gtataagctg gcttcccttc attttcagga
    48721 cagtccactg aaacctcgtc cttaccgatc aaattgatca catcaaatcg aaaacctttg
    48781 acacccttgt cgcgccagaa attaacaacc ttgaaaagct ccttacggac attgggattg
    48841 cgccagttaa ggtcagcctg ggtctcatca aataggtgaa gatagtattt cccagtatcc
    48901 ccgaaaggcg cccatgcaga accaccaaac ttagactgcc aatctgttgg ttggtcttgg
    48961 atgaagaaaa agtcttgata atacttatca ccagctaggg ctttctgaaa ccattcatgc
    49021 tctgtcgaac aatgattaag taccatgtcc agcataaagt caatcttgtg ctctttaccg
    49081 atacacacca tttcctcaaa atcagccata tcaccaaaaa gaggatccac tgccatataa
    49141 tctgaaatat cgtaaccatt atcccgttga ggacttggat agaatggatt gagccagacc
    49201 atatccacac ctagtttggc tagataggga atttttccga taatcccacg gaaatcccca
    49261 ataccgtttt cagtggtgtc tttgtaagat tttggataga tttgatagac tacttttcct
    49321 ttatcaagtg tcatctgttt ctccttttct gataaaaggg aggaagcagt cttccgtccc
    49381 tatttgtgct atttcaatta tactcaatga aaatcaaaga acaaactagg aagctagcca
    49441 caggttgctc aaaacactat tttgaggttg cagatagagc tgacgtggtt tgaagagatt
    49501 ttcgaagagt attagattcg tgtagcgacc atgagagatg ctccagcttg gatcgttgtc
    49561 ggataagttc cgggaatagt cgctgtataa gcatcttggt tggtgatgat aacaagagtt
    49621 tctgtcacca gacctgcagc cttaatgaca tccatatcaa aacgaatcag ttgctggcca
    49681 actataacgt gatctccttg gactacaaga ctttcaaaac ctttgccatc aagacctact
    49741 gtatccatac cgatgtggat gagcaattca actccctcgt cagagacaat gccgatggca
    49801 tgcttggtag ggaaaagaac cgtcactgtt ccattaactg gagaggtcaa ctcaccttgg
    49861 cttggttcaa tgaccagacc ttgccccatg acacctgatg caaaaacagg atccgtcgct
    49921 tgactcaatt ctttcacttg gccagttagt gggctgataa tttctaccga agtaagttct
    49981 actagttcat ggctcacaaa ttctgcttct tcttgagcaa cgaattctgc ctgcaagttc
    50041 gtatcgccct ctgtttttgt aaagagacca gccttgcgga agaagaaagt caagagcatt
    50101 ggaacaacaa tcgcaactag catagttcct gcaaatggca gcatgtattg aggttgaata
    50161 gagagaatac ctggcaaacc accgatacca atagaagccg cagttacatt aaaagtaacg
    50221 gataacatgc ctgcaagggc tgaaccagtc atcccagcaa caaatggata aatatatttt
    50281 acgttaaccc caaaaagagc tggttctgta acaccgagat aggctgaaat ggttgcagga
    50341 agtgaaacct gagcctcacg ctcatcatgg cgatgcatga aataataggc aaacacggct
    50401 gagccttgag caatattaga aagagcaatc ataggccaga gggcagtgcc accagcatcc
    50461 gcaatcaatt gtgtatcaat ggcattggtc atgtggtgca gacctgtgat gacaaatgga
    50521 gcgtagaggg caccaaaaat tgcaccgaag agccatttta caggacccgt taaacctgcc
    50581 aatacaactg atgaaagtcc ttgtccaatt gtccaaccga ttggccccaa gacagtatga
    50641 gccaaaatca aggctggaat caatgacaaa aaaggtacaa aaatcatgga aatgacttct
    50701 gggatatgtt tgcgccaaaa aatttcaaga taagacagac tcaaacctgc aagcaaggct
    50761 gggataactt gggcttggta accaatacga ttaacagtaa aataaccaaa attccaaacc
    50821 cagtttgctg cgatatcagc tgctggcgtt gaagcaaccg cataggcatt gagcaactga
    50881 ggcgatacca aacagattcc gagaacaatt cccaaaattt ggctggttcc catcttacga
    50941 gaaacagacc aagtaatccc tactggtaag aactggaaga tagcttcacc aggcaaccag
    51001 aggaagtgat taacccctgc ccaaaactga gaggattctg tgatggtctt gccatccaac
    51061 atcgaccaat ggacaccttc caagacatta cggaaaccga ggatcaatcc accaacaatc
    51121 aaggctggaa taatcggagt aaagatttcc gccagagtgg tcataacacc ttggaccacg
    51181 ttttgattac tcttagctgc agacttggct gcttctttgg aaacaccctc aatacctgaa
    51241 acggctgtaa aatcattata aaagatgggc acgtcatttc caatgattac ctgaaattga
    51301 cctgcatttg taaaggttcc tttaacagct ggaattgact cgatagcttt aacattagcc
    51361 ttcttatcat ctcctaaaac aaaccgcatc cgtgtcgcac agtgggtgac ggcagtcaca
    51421 ttctctttac ctccaattgc ctgaagcaaa tctttggctt cttgttcaaa ttttcccatt
    51481 tgattctcct tatacttgtc aaagcaagtt tgatttgata agtatattgt aaacgatacc
    51541 aaacttgtct gcaacttgtt tggtataaaa agggtgtttt tttgtaaatt ttctagcact
    51601 tgtatggtaa aatagataca ggtgttcatt aaactagact aaaaacctat ttaagaaagc
    51661 aaaatgaaaa aataccaaca attatttaag caaatccaag aaaccattca aaacgagact
    51721 tacgctgtcg gagattttct tcctagcgag cacgacctta tggagcaata tcaagtgagt
    51781 cgtgataccg tccgaaaggc cctgtctctc ctccaagagg aaggattgat caaaaagata
    51841 agagggcaag gttctcaagt cgtcaaagaa gaaaccgtca atttccctgt atccaaccta
    51901 accagctacc aagaactagt taaagaactt ggactgcgct ctaaaaccaa cgtggtcagt
    51961 ctggacaaga ttattattga taaaaaatcc tcactgataa ccggtttccc agagtttcgg
    52021 atggtttgga aggtggtccg ccagcgtgtg gtggatgatc tggtatccgt tctggatacg
    52081 gactatctgg atatggaact aatcccaaat ctcactcgcc aaattgctga gcagtctatc
    52141 tattcttata tagaaaatgg cctcaaactc cttattgatt atgctcagaa ggaaatcacc
    52201 attgaccact caagcgaccg agacaagatt ctcatggaca ttggcaaaga cccttatgtc
    52261 gtttcgatta aatcaaaagt ctatctccaa gacggacgcc aatttcagtt taccgaaagt
    52321 cgccataagt tagaaaaatt tagatttgta gattttgcaa aacgcaagaa ataaaagact
    52381 gagacaccag atctcagcct ttttctttat cccaaaacta attcatcact gaccagactt
    52441 tggccactat ttttctggaa gagatgcata aggtcttcaa ctgtcagatg ctttttctct
    52501 tctcccttga catccaccac tatcttgcct tgatagagca taatgagacg attgccgtac
    52561 tcaatcgcat gattcatatc atgggtaatc atcaaagtcg tccactgatg gtgctccaca
    52621 atcttttgcg tcaagtccat caccatttga ctggttttcg ggtcaagtgc cgcagtatgt
    52681 tcatccaaaa gtaaaagttt tggtttcatc agatctgtca tgactagagt caaagcctgt
    52741 ctttgtcctc ctgagagata ttgagtatct acttttaagc ggttttcaag accaatattc
    52801 aactccttca aggcctcttg gaactggatt ctatccttct ccttcacgcc ccaaccaagc
    52861 cctctcttct gcccgcgtct caaggcaata gccatattct cctcaatcgt caaacgagaa
    52921 gctgttccca tcttaggatc ttgaaaaaca cgggcaatat ccttggctct ctttctgaca
    52981 ctcagatttt taatggattt ccctgccagt agaaggtccc cttcgtccac cgatagattg
    53041 ccagccaaga tattcatcaa agtggatttt cctgctccat ttccaccaat cacagagata
    53101 aaatctccct cttcaacctc taaatctaat cctttgagga catggttctc attgaccgtc
    53161 cctgcttcaa atgtcttgtg aatattttca agtgttaaca aacttgccat aactttctcc
    53221 tcctattcat ttctcaattt cagaccacga atctttaatc tcttttgcaa ttctggtgca
    53281 aataaaacca aagcgagcaa gattgcatta aagagacgta ccaagttttg atctagattt
    53341 ggaatttcat agatatttaa aataatcaaa cggtaaacaa tagcaccgat tccgatggat
    53401 aataagcggc ctccaatggt caagtcgtgt atcaagactt ccacaataat caccgagctc
    53461 aaaccaacaa caatggttcc tgtcccagaa gtcacatctg aaaatccatc attttgggca
    53521 aacaaggaac cacatagagc aatcaaaccg tttgaaatca tgtaaccaac aatcttcatg
    53581 gtgtctacat tgactccatt ggcctcactc atcgggatat tatccccagt cgaacgcaag
    53641 accaagccaa tctctgtttt catcaaaagg gtcaagacca aacaaacaag taaaaaacag
    53701 gccaaactaa gtgagaaaac agttccttca tttgtcagac ccaagctcgc caactgttta
    53761 aagacggttg cagaatctcc caaggaaaga ttgggtacac tgcccatgat tttaatattg
    53821 attgaataga gccctgtcaa ggtcacaatc cctgtcaaga gagctgggat tttcatcttg
    53881 gtgtggagca ttcctgatac aagacctgct accatacctg ccagcaaagc aagtaaagtc
    53941 gcaagccaag gatttgcccc tgcctgtatc tgtgatacaa cgactgctgc ccccagagga
    54001 aaggctccct cagcagtcat atccgcaata tccaaaatac ggaaagtcaa gtagacccca
    54061 atcgccacaa tcgaccacaa caaaccttct gataaactag ataacacaaa attcatctta
    54121 atcctcctta ttccttgcct tctaacttac taatatcaat tcccaatgca tctgccattt
    54181 cttgattagt atgcaattcc agtttttcag gcaactcaac tgctatattt tctggtttct
    54241 caccttttaa aacatgaatc agcatgcgag ctgtctgtct tcccaattct tcataattgg
    54301 ttccgtagtt atacaagcca ccaacagcaa tcatttctgt tgaaccaccg aatactggaa
    54361 ccttgtgttt aatagaaacc tgcttaactg tttccatggt taacgaaatg atattatcag
    54421 ttgggacaaa aaccatatcc acctctgcca tcaaactctc tgctgccgcc ttgacgttat
    54481 tactgtccaa gattgttttt tcaacaacgg taaagccttt ttctttcaga agtcgcttag
    54541 cttcatcctt ttgaacaacc gaattgggct cgctctgagc agaaggcctt gcgatggcta
    54601 gaacgacatc gctggattct gctagttgct gggaaatcgt ttttagattt ctttgttctc
    54661 cctgtgcatt ttgcaaatca atctcaatat tttccccctc aacatagcct tgtttggcca
    54721 gctcatccac aaatccttct ctagtagcgt ctaaggactg gtgggtaata aactgcgaaa
    54781 taccgatacg aaagacatct cttttcttat ctgccttcaa ctgatgcaaa ctgatcagag
    54841 aagtcaagag gatccccact accaagaggg ggctagtgat tttcgaacaa ctttcataat
    54901 gaaactcctt ctcagaaaag ataaaacaaa aaaccgccta gatactatct aaacggatac
    54961 ttgaaataac ctaacaagtt acaacttagc tagtccattt agatattcat ctatatggac
    55021 cacaatcaaa aatcttagcc acatagatac aaacaaattg cttgaactgt ttgcatccat
    55081 ggtgacatgt ctacaaacac tatctaaagt agctaaagta aaagttttga ttcatagtta
    55141 cggcttgtct cttattcatt tctttttctg ctttcttttt tgatgcttcc tgtcctacca
    55201 cctttttcat cacctgtcaa taacatttca taatttttaa aatttttcaa aaattctttc
    55261 aggaagcttt caaagttttt caaatcagtc caacaaaaaa agttttataa tttccataaa
    55321 aattgacatg gaaattataa aaccattact agtttagtcc tttttgataa cgtgccaatt
    55381 cggcttggtt cgcccaaaca tagtgacctg gacggatttc taccatagat ggcttatcag
    55441 tctcatagtc gtgttgactt gggtcgtaaa ccttcaagac cttcttacgt tccaagattg
    55501 gatctgggat tggtaccgct gaaagcaagg cttgagtata tgggtgaatt ggattgttaa
    55561 acaattcttc tgtttctgca acctctacaa taacaccctt gtaaataact gcgatacgat
    55621 ctgaaataaa gcgaacaacc gacaagtcat gggcgatgaa gagataggtc aggccgagct
    55681 ctttttggaa ttttttgagc aagttcaaga cttgggcacg tacagaaacg tccaaggctg
    55741 aaattggctc atctgcaata acaaagtctg gttgcatgac caaggcacgg gcaataccga
    55801 tacgttgacg ttgaccgcct gagaattcat gagggtaacg agtcaagtgc tcagcaagaa
    55861 gacctacttc acggataata ttttgaactt tctctttacg ttcttcttca tccttaaata
    55921 aacggtgatt gtaaagacct tcagaaataa tataatcaac agtcgcacgt tcattcaaac
    55981 ttgcggcagg gtcttggaaa atcatctgga ttcgacgaat caattccgca gcttgttcac
    56041 gcgatttctt accattaatc ttttgaccat caaaaatgat atctccatta cttgtatcat
    56101 ttagaccgat gatagcacga ccaatagttg ttttcccact accggactca cctacaagcg
    56161 agaaagtttc tcccttgttg ataaagaagt tagcattttt aaccgcgaca aacttcttac
    56221 ttccttcacc gaaggaaatt tctaaatctt tgatttctac taatttttca gacatttcct
    56281 tcctcctagt cagccagatg ggcaaatccc attttttcac ggatcttatc atggagattt
    56341 gcaatcacag ctggtttttc tacttttgga gcatcctcat gaagaagcca agttttagcc
    56401 caatgtgtct ctgatactga gaattgagga gctttttgtt cgaagtcaat ctgcattgcg
    56461 tagtcagaac gcaaggcaaa agcatcccct ttcaggtcag tataaagtga cggaggtgtt
    56521 cctgggattg agtaaagttc ccctttatca tcagcaagct gaggcaagct agacaagaga
    56581 ctccatgtat atggatggcg agggtcatag aagacttcct caaccgttcc atactcaacg
    56641 atttctcctg catacataac cgctacctta tccgcaatac ttgccaccac accaaggtcg
    56701 tgggtaataa agattgttgt gaaatgatac tcgttttgta aagattttag caaatcaata
    56761 atctgagctt gaatagtcac atccaaggca gttgttggct catcacagat caagacatca
    56821 ggtcggcagg caagggcaat cgcaataacg atacgttgac gcattcctcc agaatattgg
    56881 aatgggtatt cattaaaacg tctatctgcg tctggaatgc caaccttatt catgtagtca
    56941 atggccaatt ctttcgcttc tttagctgtt tttccttggt gttttacaat aacttctgta
    57001 atctgactac caattgtttt aatggggtcc aaactagtca ttgggtcctg gaagatagtc
    57061 gcaatcttag caccacgaat ttgttcccaa tccttgtgag aagataaagc tgtcaagtcc
    57121 tgaccacggt agtcaatact accttgggca atacgaccat tttcttcgag catacctgtg
    57181 aaggtctttg tcaaaacaga tttacctgat cctgactcac ctaccaaggc taatacttct
    57241 ccttcgacta gttcaaggga aacgccgcga atggctgtca atactttgtc acgaacgtca
    57301 aattccacga caatatcgcg agcagtcaaa attacatttt tttcttttgt catttctact
    57361 cctatctatg tgtacgtgga tcactagcat ccgctaagtt ttgaccaact acgaaaaggg
    57421 acaaggatac caagacaagg gttgtcaatg gaatccagaa caagtaagca ttggttgtta
    57481 cgttttgtga ataatccgaa atcaaacgac ccaaacttgg cactgtaatc ggtaatccaa
    57541 gaccgaagaa agacaagaag gcttcgtatg agataaagct tggaagcatt tgagtcatgg
    57601 ttgtcacaat aacagatacc aattgaggca tgatattttt ggcaacaatc ttcaaggttg
    57661 gtgttcccaa agtacgtgac gccaagttgt attccaagtc acgatagcgc aagatttgca
    57721 cacggatcat gaaggcaata ccaatccatg ttgttacgct catggcaaaa atcagattcc
    57781 agaatccagc tccgattgag taagttaaga caataacaat caaaagaggt gggatgtttg
    57841 agatgacgtt gtaaacttcc atcatgacac ggtcaactga ttttgaaata ccccaaatac
    57901 caccgacaaa aacaccgata accaagttaa tcactgtcgc aatcacagaa atgaggatgg
    57961 agttacgagc tccgaaccag acaccgtcaa agagcgattt accgttactg tcagtaccga
    58021 accaatgctc cgcatttggc ttgatataac gaacactaaa gtcgtttacc ttgctgacat
    58081 cattgaaatc aaacttagaa aacattgggt agatgaaact tatcaaaatg atggctacca
    58141 agattcccaa catgactaca gttgattttt tcttcataaa ttgtttaaac actgatttcc
    58201 agtaagaata tgctggcgca tcaatagttt cagaggcaaa atcgtcacgt tttacaaact
    58261 gaaatttttc tttatcgatt gtagacatta tttgcctcct ttctcagtca atttaatacg
    58321 tgggtcaata atagtcatcc aaatatctcc caaaagacgt gagaagatag aaatacatgt
    58381 aaagatgaag acaagaccaa cgaccataga gttattagat gcttttacag agtcaatcaa
    58441 cattttaccc atacctggga aggcgaagac tgtttcagta agggttgcac caccgataac
    58501 cccaataatg gcaccaggaa ttcctgaaac cagcggaacc atggcatttt taaagatgtg
    58561 tttgtttgaa atttcttttt cagacaaacc ttttgcacga gcgaaacgaa caaagtcttg
    58621 agattgcaag tcaatcatgt aacgacgaat ccaaatggct gtaccaggag cacccaacaa
    58681 accaaggatg actgctggta aaacgtaaga acgccaatct ccagctccca agatagggaa
    58741 tgaatctgga agggaaatag atgatccaat caatcgaacg atgtaaacca aggcaatcgt
    58801 tggaagagca agcaagaagg tcaaagcccc tgttgagagg ctatcaatcc aagtgttctt
    58861 gaaacgagcc atggctgaac caagtggcac ggcaagagca taggcaagaa ccaaaccaat
    58921 caaaccagta atagcagagc tgacaatcat agatggatat tggtaattac tttcagtcgc
    58981 tgtataagga tcatctttcc catagctagc tacttcacga gagtcagcct gactaggtga
    59041 cttgtaggtt cttgagtaaa tatttacaga agacgttttc ttacctgttg ggaactgaac
    59101 ttgggcagtt ttggtttgtc cttgaccttg agtaataacc tgaagaactg gtgtattagc
    59161 ataggttggg taagagtcac ctaaattcaa gttcacaaag ttttgatgaa caaatgggaa
    59221 ctgactgtta aagtacaaga gatatttatg tttagttcct gaaccgacca atgaccatcc
    59281 gatagctgga tcattttcaa aacgaaggta gcgtttcaag tctggatttt cagggtcttg
    59341 gattttattt gtatggtcaa tgtcaatcaa gttagcatag aagtgaaaaa cacgttcaaa
    59401 aattggaatt tcacgagtag catagaattg accactttca gtaaattctc ccaaagtcca
    59461 accatgacct aattgattga tgtacttttc ataaatagct ttattggtcg catttgcttc
    59521 tactgttaca gaagaatcca tgctacttgc cttttcttgc aactctttag tatcgtaata
    59581 ctcaatgtag cccatacgct caaacacagt attttcatag ttatcacgtt tatcagccgt
    59641 tgtcgcaatt ttattatagt taggatcctg cttgaaaatc aattttcgag gaaccaaggt
    59701 atagataatc gtgtaggtca aagtcgttac taagaaaatc gaaaccaatg accgcaaaac
    59761 acgcataaaa atatattttt tcatattatt tcctttaaaa atcccaaaag aaccttctcc
    59821 tcatggagag aaagttctat tagaaattat ttacttcaca tgacttgcca attctttttg
    59881 agctttctca tttgattcag ctttttcttt caaccatttt tcacgagctt tttcatactc
    59941 ttccttagtc accgctttat cttgtaattt caaatatttg aagtaaacat ctgacccctt
    60001 agagcctgtt tgcgcagaag ctccagtaaa tggaacaatt cgtgaaagca ctggtgctgc
    60061 accagaagaa gccatagcag gaataaagag tgaactatct gtcaaccatg cttgagccgc
    60121 tgcatatttt tcataacgga cattcaagtc gcttgtctct ttggcagctt catcaactaa
    60181 tttatcgtat tctttcaaac caacttgaac tactgaaggg ctatttggat tatcaaatcc
    60241 taaatatgtt tttgtagttt cactgctagt tgtttttaaa atatccaggt aagtagatgg
    60301 gtcttgatag tctggccccc atgaaactcc tcccgataca tcccaatcct cagatgaagc
    60361 attggcagca tagtaagtaa tattaaggaa ttcatcactt gtcatttgtt gaatatcaac
    60421 aacgacattt tcaacaccaa gaactgtttc tacagattgt ttaaaggact gaatacgaga
    60481 tatatagttt tttgatgctt ggtctactgg aacgtccaga tgaataggaa actgaacgcc
    60541 gtctgcttct aaagctttct tagctttcgc aaactctgcc ttggccttgt cagcattgaa
    60601 taaaccatcc tgcccatcag ctaaattcac acctttccac tcatcaccat aagcaggaag
    60661 ttgagcagcg actaaatcac caaaggtctt ctcaccagct gaaacaaagt ctggttttac
    60721 aaataaatta cgaactgcta aagctgctcc atctttacca ttgatttgag ctgagtaagc
    60781 tgagcgatca agagcaaaat tcaaggcttg acggaaatct ttgttaagca atgccttctt
    60841 agtagctact ttctctgaat ctgtagtttt agaagtatag ttgtaacttt ggcgatcaat
    60901 attcacaccc agaccagcaa tcccagagcc tgattgtgtg taatagatat tgtccttgta
    60961 ttcttctgca accttagaat agttggagct ggtagggtaa agacgggcat aactataggc
    61021 tccactagtg aagttacgct ctagcgactc ctgatctgat ccatcatagt aagctagatt
    61081 gatagtatct aggtggacat tttctttatc ccaatattgc tcatttttta caaactctac
    61141 agaagatttt gcagtcaacc ctttcaacaa gaatggacca ttataaagca aggatgtcgg
    61201 atctgttggt ttagcaaaat cgcttccttt tgatgtttcg aattcttcat tcagaggcca
    61261 gaaaatagaa taggtcaact tagagttcca gaacggttca ggctggttca aagtgtattg
    61321 taacgtataa tcatcaaccg ccttgacacc aactgttgaa aaatctgttg aagttcctga
    61381 tagataatct gccaagcctt taaccgaatt ttcagctaaa tacatagctt ctgatttttt
    61441 atctgctgcg tgttttaaac cgttcacgaa atctttagcc gtcacctctg catattcttc
    61501 tccatcagag gtaaaccatt taaccccttt acgaatctta taagtgtagg tcaaaccatc
    61561 cttagagact tcccaatcct ctgcaactgc aggagcaaga ttaccgtaat tatcgttagt
    61621 gaataaacca tcaatcccat ttgaagtcac tactgttgta ctatttttac ttgaaatcag
    61681 gtagtccaag gtttctgggt ctgctgtata aacatagcca taagctttag gggctgatga
    61741 atcagatgat tttgaagaac tgcatgctgc aagtacacct gctgctaata aaacaagacc
    61801 tgctgtagca aatacacgat tttttttcat tttctactcc tctgtttatg tgaattatag
    61861 attgacaacc attatatcac attatccatt aaaaatcaaa caaattttca gaatatttag
    61921 gcttgttggc acaaattttt catttttttt gaatatatga ttcaaattgt cgttcgaagt
    61981 gtcaaagact acagtgaaaa taggaaattt gacgcagaaa ctttggagct taggaagaca
    62041 tacagtaaaa tgaaatacgg acggaacaat gtgattttgg aattcaaatt aaattataac
    62101 aatattgtag aagtatcatt ctagtattca agattcagtt tactatgtct tttcacacca
    62161 accttattcc gaattcaatt actttcgtga tttacacata tagattaaga ctatcttt
//
LOCUS       DAVMGZ010000002        50037 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000002 DAVMGZ010000000
VERSION     DAVMGZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50037
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.002"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(522..614)
                     /locus_tag="VYQ93_000062"
     CDS             complement(522..614)
                     /locus_tag="VYQ93_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV3334825.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            775..1746
                     /gene="ruvB"
                     /locus_tag="VYQ93_000063"
     CDS             775..1746
                     /gene="ruvB"
                     /locus_tag="VYQ93_000063"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV3334826.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHAGLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            1727..2299
                     /locus_tag="VYQ93_000064"
     CDS             1727..2299
                     /locus_tag="VYQ93_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV3334827.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            2530..3288
                     /locus_tag="VYQ93_000065"
     CDS             2530..3288
                     /locus_tag="VYQ93_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV3334828.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALIKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            3297..4100
                     /locus_tag="VYQ93_000066"
     CDS             3297..4100
                     /locus_tag="VYQ93_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV3334829.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKIRGLATMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            4122..5381
                     /gene="rseP"
                     /locus_tag="VYQ93_000067"
     CDS             4122..5381
                     /gene="rseP"
                     /locus_tag="VYQ93_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV3334830.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRHKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            5394..7247
                     /locus_tag="VYQ93_000068"
     CDS             5394..7247
                     /locus_tag="VYQ93_000068"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV3334831.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPTLLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            7347..8726
                     /gene="bglA"
                     /locus_tag="VYQ93_000069"
     CDS             7347..8726
                     /gene="bglA"
                     /locus_tag="VYQ93_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV3334832.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            8918..10726
                     /gene="glmS"
                     /locus_tag="VYQ93_000070"
     CDS             8918..10726
                     /gene="glmS"
                     /locus_tag="VYQ93_000070"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV3334833.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            10845..11894
                     /locus_tag="VYQ93_000071"
     CDS             10845..11894
                     /locus_tag="VYQ93_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV3334834.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(11994..15791)
                     /gene="spuA"
                     /locus_tag="VYQ93_000072"
     CDS             complement(11994..15791)
                     /gene="spuA"
                     /locus_tag="VYQ93_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV3334835.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDSKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDLFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLIDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQEASV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            16208..16621
                     /gene="rpsL"
                     /locus_tag="VYQ93_000073"
     CDS             16208..16621
                     /gene="rpsL"
                     /locus_tag="VYQ93_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV3334836.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16641..17111
                     /gene="rpsG"
                     /locus_tag="VYQ93_000074"
     CDS             16641..17111
                     /gene="rpsG"
                     /locus_tag="VYQ93_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV3334837.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            17536..19617
                     /gene="fusA"
                     /locus_tag="VYQ93_000075"
     CDS             17536..19617
                     /gene="fusA"
                     /locus_tag="VYQ93_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV3334838.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            19721..24112
                     /locus_tag="VYQ93_000076"
     CDS             19721..24112
                     /locus_tag="VYQ93_000076"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV3334839.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGIEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNAAFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            24214..24477
                     /locus_tag="VYQ93_000077"
     CDS             24214..24477
                     /locus_tag="VYQ93_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV3334840.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            24470..24748
                     /locus_tag="VYQ93_000078"
     CDS             24470..24748
                     /locus_tag="VYQ93_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV3334841.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            24769..24921
                     /locus_tag="VYQ93_000079"
     CDS             24769..24921
                     /locus_tag="VYQ93_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3334842.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            24943..26184
                     /locus_tag="VYQ93_000080"
     CDS             24943..26184
                     /locus_tag="VYQ93_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV3334843.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            26194..26424
                     /locus_tag="VYQ93_000081"
     CDS             26194..26424
                     /locus_tag="VYQ93_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV3334844.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            26737..27459
                     /locus_tag="VYQ93_000082"
     CDS             26737..27459
                     /locus_tag="VYQ93_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV3334845.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            27676..29010
                     /gene="pepC"
                     /locus_tag="VYQ93_000083"
     CDS             27676..29010
                     /gene="pepC"
                     /locus_tag="VYQ93_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV3334846.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(29066..29977)
                     /locus_tag="VYQ93_000084"
     CDS             complement(29066..29977)
                     /locus_tag="VYQ93_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV3334847.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(30001..30804)
                     /locus_tag="VYQ93_000085"
     CDS             complement(30001..30804)
                     /locus_tag="VYQ93_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV3334848.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(30832..31821)
                     /gene="manX"
                     /locus_tag="VYQ93_000086"
     CDS             complement(30832..31821)
                     /gene="manX"
                     /locus_tag="VYQ93_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV3334849.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(32055..33074)
                     /gene="adhP"
                     /locus_tag="VYQ93_000087"
     CDS             complement(32055..33074)
                     /gene="adhP"
                     /locus_tag="VYQ93_000087"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV3334850.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKGKTDGGAHSAVVTAVSKVAFNQAVDSVRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVAPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(33413..34225)
                     /locus_tag="VYQ93_000088"
     CDS             complement(33413..34225)
                     /locus_tag="VYQ93_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3334851.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKETIKKVAEKGHQIIITTG
                     RPYRMSKDFYHELGLNTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVRRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPENQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            34415..35833
                     /locus_tag="VYQ93_000089"
     CDS             34415..35833
                     /locus_tag="VYQ93_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV3334852.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGILL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFKDLKTIFGAALGSEGLGALISDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTSIGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLASLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQAK
                     DVHVMIWILDALFILNYISMAL"
     gene            35938..36645
                     /locus_tag="VYQ93_000090"
     CDS             35938..36645
                     /locus_tag="VYQ93_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3334853.1"
                     /translation="MIKKNIWDEVLNRGKWLIIFLVGLLFSQFPLALATFLTSRKFPL
                     LQTGLLVGALSLVVLTVFIIGARKTQLASFGLSFFKAKDLARLALSYLVILAFNILGV
                     VLLHLMNETTTSNQSNINDLVQNSSLISSFFLLLLIAPICEEILCRGVIPKKLFRGKE
                     NVGYIVGAIIFALLHLPTNLPSLLIYGGMSTVLTWTVYKTQRLEMSILLHMIVNGIFF
                     CLLALVVILSRTLGISV"
     gene            36739..37719
                     /gene="folP"
                     /locus_tag="VYQ93_000091"
     CDS             36739..37719
                     /gene="folP"
                     /locus_tag="VYQ93_000091"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV3334854.1"
                     /translation="MMKSLEGVTDMSTKANHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYSYVEIEEEIQRVVPVIKAIRKESDVLISI
                     DTWKSQVAEAALAAGANLVNDITGLMGDEKMARVVAKAGAKVVIMFNPVMARPQHPSS
                     LIFPHFGFGQAFTEEELADFETLPIEDLMDAFFERALARANQAGIAQENILLDLGIGF
                     GLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDT
                     ASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            37721..39043
                     /locus_tag="VYQ93_000092"
     CDS             37721..39043
                     /locus_tag="VYQ93_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV3334855.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVIHI
                     GGTNGKGSTIAFLKNMLEKLGLRVGVFSSPYLIHYTDQISINGESIPEARLEALMADY
                     QSLLEGEAVANLQSTTEFEIITALAYDYFAAEQVEVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGIPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVSHQKSVVTGEVFDYTSVVRQGRFQTGLLGLHQIENAGMAIALLDTFCQE
                     HGRELASNDLLGQALEETSWPGRLEVVSRDPLMILDGAHNPHAIKALLATLQERFADY
                     RKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFDDSRATDESVLEEAAKSRNLNYQG
                     WQDFLEQKLTDKKEEKKTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            39024..39578
                     /gene="folE"
                     /locus_tag="VYQ93_000093"
     CDS             39024..39578
                     /gene="folE"
                     /locus_tag="VYQ93_000093"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV3334856.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMEYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            39737..40549
                     /gene="folK"
                     /locus_tag="VYQ93_000094"
     CDS             39737..40549
                     /gene="folK"
                     /locus_tag="VYQ93_000094"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV3334857.1"
                     /translation="MDQLQIKDLEIFAYHGLFPSEKELGQKFVVSAILFYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETTEDLIETVAYKLVERTFEAYPLVQEMKLELKKPWA
                     PVHLPLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIEKMRNQGIHILKESSVLTT
                     EPWGGVEQDSFANQVVEVETWLPAPVLLESLLAIESEMGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPTLKQPIRNLYNALKQ"
     gene            complement(40595..40912)
                     /locus_tag="VYQ93_000095"
     CDS             complement(40595..40912)
                     /locus_tag="VYQ93_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV3334858.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            41269..41715
                     /gene="rplM"
                     /locus_tag="VYQ93_000096"
     CDS             41269..41715
                     /gene="rplM"
                     /locus_tag="VYQ93_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV3334859.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            41735..42127
                     /gene="rpsI"
                     /locus_tag="VYQ93_000097"
     CDS             41735..42127
                     /gene="rpsI"
                     /locus_tag="VYQ93_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV3334860.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(42389..42811)
                     /locus_tag="VYQ93_000098"
     CDS             complement(42389..42811)
                     /locus_tag="VYQ93_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3334861.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            42958..43134
                     /locus_tag="VYQ93_000099"
     CDS             42958..43134
                     /locus_tag="VYQ93_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3334862.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            43535..45748
                     /locus_tag="VYQ93_000100"
     CDS             43535..45748
                     /locus_tag="VYQ93_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV3334863.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(45970..46446)
                     /locus_tag="VYQ93_000101"
     CDS             complement(45970..46446)
                     /locus_tag="VYQ93_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV3334864.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQR
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINTFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            46669..49872
                     /locus_tag="VYQ93_000102"
     CDS             46669..49872
                     /locus_tag="VYQ93_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV3334865.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNREKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKSPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRPSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKGINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
BASE COUNT        14610 a         9380 c        11433 g        14614 t
ORIGIN      
        1 ttcataataa tctcccttaa cttcacccaa tcaggtggag ttttttagct ctatttcagg
       61 cttttgggga ctattctaaa aataattttt cgatattttt cggtattttt cggattttgg
      121 tcggggaatt ggcggggact tttttagcga atataactaa gaaataggtc tgtggtcgct
      181 tcagctagtt caaactcaat ttgattgtaa gtgactgtta attgaagaac tggaactact
      241 gtaataatga ttttgcttgt ctagtagtgg cgtattaaat gagaagtaac atgtaagatt
      301 gtctcttcgt ttacgaaatc aaaatttcta tgaaattgat agaatcctta acgttctctt
      361 aatcttaacc gtcttgtcgt acagcggttg aagaaaatgt aaacctttga caaaattcgt
      421 gaactatggg ataataaaaa ggaattgagt actagttcta tatcataggc tagaaaagac
      481 cccaagctca cgaccaaata agcttgaggt cttttttgac actatttgtc cttgtttagc
      541 catttatcga ctatacgaag aacgacaccg accacaattg gtccgatgat agttttgagt
      601 actaattcca tcatgggcta tctcacctcc tttcgtaggc ggtgtagcag tgtcgtagaa
      661 tattatacca cataagtgct aaacttagga gtcacccaat caggtggctt ttttgtttgt
      721 ggcttggttt tttgatataa tagagccatg agtagaattt tagataatga gataatgggg
      781 gatgaggagt tagtagaacg cacgctccgt cctcagtatt tacgtgaata tatcggacag
      841 gataaggtca aggaccagct acaaatcttt attgaagctg ccaaaatgcg ggatgaagcg
      901 ctggatcatg tgctcttatt tgggcctcca ggtctcggga aaacgaccat ggcctttgtt
      961 attgccaacg aactgggagt caatcttaag cagacgtcgg gtccagtcat tgaaaaagcc
     1021 ggagatctgg tagctatttt gaatgagtta gagcctgggg atgtactttt tattgatgag
     1081 atccatcgtt tgccaatgtc agtggaagag gtgctttata gtgctatgga ggacttctac
     1141 atcgatatta tgattggggc tggtgagggt agtcgtagtg ttcatttgga gttaccacct
     1201 tttaccttga ttggtgcgac gactcgggct ggtatgctct ccaatccgct acgggcacgt
     1261 tttgggatta caggccatat ggagtattat gcccatgctg gcttgacaga aattgtcgag
     1321 cggacggcag atatttttga gatggaaatc actcatgagg cagcatctga gttggcccta
     1381 cgtagtcgtg ggacccctcg tattgccaat cgtctcctca agcgcgtgcg cgattttgcc
     1441 cagataatgg ggaatggggt aattgatgat attattaccg ataaggcttt gactatgctg
     1501 gatgttgacc atgaaggttt ggactatgtg gatcaaaaaa tccttcgtac catgattgag
     1561 atgtacagtg gaggacctgt tggtctagga actctttctg tgaatatcgc cgaagagcgt
     1621 gagacagttg aagatatgta tgagccttac ttgattcaaa aaggttttat catgcggaca
     1681 cggtctggac gggtggcgac tgctaaggca tatgagcact taggttatga atacagtgaa
     1741 aaataagcaa gaaatcttag aggcttttag agaaaatcca gatatgatgg ccattctgac
     1801 gatcatccga gaccttggtc tgaaagactc gtggttggca gcaggttctg tcagaaattt
     1861 catttggaat cttttgtcag acaaatcccc ttttgatcat gaaacagata tagatgtgat
     1921 tttctttgat ccagattttt cttatgagga aaccttatta ctggagaaaa agctgcgaga
     1981 ggattttcct cagtaccagt gggaattgaa aaatcaggtc tatatgcatc agcacagtcc
     2041 tcacactgct tcctatacca gttctcgtga tgctatgagt aagtatccag aacggtgtac
     2101 gacagttgga ctgcgcttga atgaagaatc agattttgaa ctctatgcac cttatggttt
     2161 ggaggatatt ttgaattttc aagttcgtcc aactcctcat ttcttagaaa atgaagaccg
     2221 aatggaactc tatcaaacac gtttatccaa gaaaaattgg caggagaaat ggaaaaattt
     2281 gatttttaaa aatacttaag gaaactttaa gctagggagt gtacactaag ttcataagtt
     2341 aagaagacct taacttaaac tcctaaaact ttttcataat aatctcccta taaaaataaa
     2401 gtcgcccaat caggcggctt atttttttga aaaatgggct tggtgcctga gaataaatag
     2461 cttagtgata gaagaaaatg gggaaatatg gtataatgaa acgatagatt tttgaatagg
     2521 aataagatca tgtttggatt ttttaagaaa gataaggctg tggaagtaga ggttccgaca
     2581 caggttcctg ctcatatcgg catcatcatg gatggcaatg gccgttgggc taaaaaacgt
     2641 atgcaaccgc gagtttttgg acataaggcg ggcatggaag cattgcaaac cgtgaccaag
     2701 gcagccaaca aactgggcgt caaggttatt acggtctatg ctttttctac ggaaaactgg
     2761 acccgtccag atcaggaagt caagtttatc atgaacttgc cagtagagtt ttatgataat
     2821 tatgtcccgg aactacatgc gaataatgtt aagattcaaa tgattgggga gacagaccgc
     2881 ctgcctaagc aaacctttga agctttaatc aaggctgagg aattgactaa gaacaacaca
     2941 ggattgattc ttaattttgc tcttaactat ggtggacgtg ctgagattac acaggcgctt
     3001 aagttgattt cccaggatgt tttagatgcc aaaatcaacc caggtgacat cacagaggaa
     3061 ttgattggca actatctctt tacccagcat ttgcctaagg acttacgaga cccagacttg
     3121 attatccgta ctagtggaga attgcgtttg agcaatttcc ttccatggca gggagcctat
     3181 agtgagcttt attttacgga caccttatgg cctgattttg acgaagcagc cttgcaggaa
     3241 gctattcttg cctataatcg tcgccatcgc cgatttggag gagtttagga ggaaatatga
     3301 cccaggattt acagaaaaga accttgtttg cagggattgc cctggctatt ttcctaccaa
     3361 ttttaatgat tgggggcctc ttgcttcaga tagcaatcgg aatcatagcc atgctagcca
     3421 tgcatgaact tttgaagata agaggtctag cgaccatgac gatggagggc ctcttgactc
     3481 tctttgcaac ctttgcatta accattccct tggagaatta cctgactttt ttgccagttg
     3541 atgggaatgt ggttgcctat agtgttttga tttcaatcat gttaggaacg accgttttta
     3601 gcaagtctta tacgattgag gatgcggttt tccctcttgc tatgagcttc tacgtgggct
     3661 ttggatttaa tgctttacta gatgctcgtg ttgcaggttt ggacaaggct ctcttagcct
     3721 tgtgtatcgt ctgggcgaca gacagtggtg cctatcttgt tgggatgaac tatgggaaac
     3781 gaaagttagc accaagggta tcgcctaata aaacccttga gggtgccttg ggtggtattt
     3841 taggagcaat tttagtaacc attatcttta tgatagttga cagtacagtt gctcttccat
     3901 atggaattta caagatgtca gtctttgcta ttttctttag cattgctgga caatttggtg
     3961 atttactaga aagttcgatc aaacgtcatt ttggtgttaa ggattctggg aaatttatcc
     4021 ctggacatgg tggtgttttg gatcgtttcg atagtatgtt gcttgtattt ccaatcatgc
     4081 acttatttgg actcttttaa tcaaaagacg gaggaaacgc tatgctcgga attttaacct
     4141 ttattctggt tttcgggatt attgtagtgg tgcacgagtt cgggcacttc tactttgcca
     4201 agaaatcagg gattttagta cgtgaatttg ccatcggtat gggacctaaa atttttgctc
     4261 acattggcaa ggatggaacg gcctatacca ttcgaatctt gcctctgggt ggctatgtcc
     4321 gcatggccgg ttggggtgat gatacaactg aaatcaagac aggaacgcct gttagtttga
     4381 cacttgctga tgatggtaag gttaaacgca tcaatctctc aggtaaaaaa ttggatcaaa
     4441 cagccctccc tatgcaggtg acccagtttg attttgaaga caagctcttt atcaaaggat
     4501 tggttctgga agaagaaaaa acatttgcag tggatcacga tgcaacggtt gtggaagcag
     4561 atggtactga ggttcggatt gcacctttag atgttcaata tcaaaatgcg actatctggg
     4621 gcaaactgat taccaatttt gcaggtccta tgaacaattt tatcttaggt gtcgttgttt
     4681 tttgggtttt aatctttatg cagggtggtg tcagagatgt tgataccaat cagttccata
     4741 tcatgcccca aggtgccttg gccaaggtag gagtaccaga aacggcacaa attaccaaga
     4801 ttggctcaca tgaggttagc aactgggaaa gcttgatcca agctgtggaa acagaaacca
     4861 aagataagac ggcaccgact ttggatgtga ctatttctga aaaggggagt gacaaacaag
     4921 tcactgttac acccgaagat agtcaaggtc gttaccttct aggtgttcaa ccgggggtta
     4981 agtcagattt tctatccatg tttgtaggtg gttttacaac tgctgctgac tcagctctcc
     5041 gaattctctc agctctgaaa aatctgattt tccaaccgga tttgaacaag ttgggtggac
     5101 ctgttgctat ctttaaggca agtagtgatg ctgctaaaaa tggaattgag aatatcttgt
     5161 acttcttggc aatgatttcc atcaatattg ggatttttaa tcttattccg attccagcct
     5221 tggatggtgg taagattgtg ctcaatatcc tagaagccat ccgccacaaa ccattgaaac
     5281 aagaaattga aacctatgtc accttggccg gagtggtcat catggttgtc ttgatgattg
     5341 ctgtgacttg gaatgacatt atgcgactct tttttagata atcgaggaat attatgaaac
     5401 aaagtaaaat gcctatccca acgcttcgcg aaatgccaag cgatgctcaa gttatcagcc
     5461 atgctcttat gttgcgtgct ggttatgttc gccaagtttc agcaggtgtt tattcttatc
     5521 taccacttgc caaccgtgtg attgaaaaag ctaaaaacat catgcgccaa gaattcgaaa
     5581 agattggtgc tgttgagatg ttggctccaa cccttcttag tgcagaattg tggcgtgaat
     5641 caggtcgtta cgaaacctat ggtgaagacc tttacaaact aaaaaaccgt gaaaaatcag
     5701 actttatctt aggtccaact cacgaagaaa cctttacagc tattgtccgt gattctgtta
     5761 aatcttacaa gcaattgcca ctcaaccttt atcaaattca gcccaagtat cgtgatgaaa
     5821 aacgcccacg taatggactt cttcgtacac gtgagtttat catgaaggat gcttatagtt
     5881 tccacgctaa ctatgatagt ttggatagtg tttatgatga gtacaaagca gcctatgagc
     5941 gtattttcac tcgtagtggt ttagacttca aggctattat tggtgacggt ggagccatgg
     6001 gtggtaagga tagccaagaa tttatggcca ttacatctgc tcgtacagac cttgaccgct
     6061 gggttgtctt ggacaagtca gttgcctcat ttgacgaaat tcctgcagaa gtgcaagaag
     6121 aaatcaaggc agaattgctc aaatggatag tctctggtga agataccatt gcttactcaa
     6181 gtgagtctag ctatgcagct aacttagaaa tggcaacaaa cgagtacaaa ccaagcaacc
     6241 gtgttgtcgc tgaagaagaa gttactcgtg ttgcaacgcc agatgttaaa tcaattgatg
     6301 aagttgcagc cttcctcaat gttccagaag aacaaacgat taaaaccctc ttctacattg
     6361 cagatggtga gcttgttgca gcccttctag ttggaaatga ccaactcaac gaagtcaagt
     6421 tgaaaaatca cttgggagca gatttctttg acgttgctag cgaagaagaa gtggcgaatg
     6481 ttgttcaagc aggatttggt tcacttggac cagttggttt gccagagaat attaaaatta
     6541 ttgcagatcg taaggtgcaa gatgttcgca atgcagttgt cggtgctaac gaagatggct
     6601 accacttgac tggtgtgaac ccaggccgtg attttactgc agaatatgtg gatatccgtg
     6661 aagttcgtga gggtgaaatt tccccagatg gacaaggtgt ccttaacttt gcgcgtggta
     6721 ttgagatcgg tcatattttc aaactcggaa ctcgctactc agcaagcatg ggagcagatg
     6781 tcttggatga aaatggtcgt gctgtgccaa tcatcatggg atgttacggt atcggtgtca
     6841 gccgtcttct ttcagcagtg atggagcaac acgctcgcct ctttgttaac aaaacgccaa
     6901 aaggtgaata ccgttacgct tggggaatca atttccctaa agaattggca ccatttgatg
     6961 tgcatttgat tactgtcaat gtcaaggatg aagaagcgca agccttgaca gaaaaacttg
     7021 aagcaagctt gatgggagct ggttatgaag tcttgacaga tgaccgtaac gaacgtgtcg
     7081 gtgttaaatt tagcgatagt gacttgattg gtcttccaat ccgtatcact gttggtaaaa
     7141 aagcggccga tggcattgta gaagttaaga tcaaggcaac tggtgacacc attgaggttc
     7201 atgcagataa cgtgcttgaa acgcttgaaa tcctaagcaa gaaataaaaa ctataatcag
     7261 aagaaaaaca aggaaaaaat gtaactagtt tttaccttgt tttttctgta taatgggaga
     7321 aatgagtaga taaagaggta aatgttatgc taagatttcc aaaggatttt gtctggggat
     7381 cctctacttc tggaccgcag acagaaggac gtgtagctgg tgacggtaag ggagacaatc
     7441 tctgggatta ctggttccaa gtggagccaa atcgttacta taatgggatt ggttcagata
     7501 agacatcgac tttttatgaa aattgggagc gggatattga gcttttgtta gagactggtc
     7561 acacagtctt tcggacttct attcagtggt cacggatttt tccacaaggc tgtggaaaag
     7621 tcaaccctca aggtgtggat ttttatcgta aggtctttga ggctattaag gctaaaggaa
     7681 ttcgtctgtt agtcaatctc tatcattttg atttaccttt tgcccttcaa gaggatggtg
     7741 atggttggga aaataaggcg acagtctcag cctatgaaga ctatgctcgt ttttgttttg
     7801 agacttatgg agatttagtg gatcaatgga ttacctttaa cgagcccatc gttcctgtag
     7861 aatttggtta tttttatgat gcccattatc cacataaggt ggatgcagag gcagctgtta
     7921 aggtagccta tcatacacaa ttggccagca gccgggctgt taaggcttgc catgaacttt
     7981 tgcctgattc caagattggg attgtcctca acttgacacc ggcttatcca cgtagccagc
     8041 atcctgctga tgtcaaggca gctcgtattg cggccctttt tcaggcccaa tctttcttag
     8101 atccatctgt cttggggact tatccacagg agttggtaga aatcttgcat gaacacggtc
     8161 ttttacctga tgctacagag gaagagttgg aactcattcg tgataatacg gtggacttcc
     8221 ttggtgtgaa ctactatcaa cctttgcgtg ttatggctcc tcgatttgct aagcatccag
     8281 atagtccact cttaccagaa catttttacg agccttatgt gatgcctgga cgtaaaatca
     8341 attctcaccg tggttgggag atttatgagc aagggattta tgacatcgcc caaaatatca
     8401 aggaaaatta tggcaatatt gagtggatgt tgactgagaa tggtatgggt gttgaagggg
     8461 aagaaaaatt ccgtcaagat ggaatgattc aagatgatta ccgtattgac tttgtaaaag
     8521 gtcatcttcg tgaacttcac cgtgccattg aagatggtgc caactgtaag ggctacttga
     8581 tttggacctt tattgactgc tggtcatggc tcaacagcta taaaaatcgc tatggtttgg
     8641 tcgaattaga cttggaaacg caagaacgtc gtctgaagaa atcaggccac tggttcaaag
     8701 aattaagcga taataatgga ttttaaaaag gactctgact gattatccca atcggtcaga
     8761 gttttttgct tacaggagct taatttgaac tataccaatt tttactcttg acaagtgaaa
     8821 gaaacaagta tatactgttt ttgctgattc tataaaagat gaattagact ataccaattt
     8881 taaggagaaa gcacagcttg cctgtgtcgt atatactatg tgtggaattg ttggtgttgt
     8941 tggaaacaca aatgcaactg atattttgat tcaagggctt gaaaagcttg aataccgtgg
     9001 ctatgattct gcgggaattt ttgtcctaga tggtgctgat aaccatttgg tgaaggctgt
     9061 tggtcgtatc gcagaattgt ctgccaagac agctggtgtt gagggaacaa ctggtatcgg
     9121 acatactcgt tgggcaactc atgggaaacc aacggaagac aatgctcacc cacaccgctc
     9181 tgagacagaa cgttttgtct tggttcacaa tggggtgatt gaaaactacc ttgaaattaa
     9241 agaagaatac cttgctgggc accacttcaa agggcaaaca gatacggaaa tcgccgtaca
     9301 tttgattgga aaatttgcgg aagaagacgg tctctcagtt cttgaagcct ttaaaaaagc
     9361 tcttcatatt atccgtggtt catatgcctt tgccttgatt gactctgaaa atccagatgt
     9421 catctatgta gcgaaaaaca aatctccact tttgattggt cttggggaag gctacaatat
     9481 ggtctgctca gatgctatgg ctatgattcg tgaaaccaac caatacatgg aaattcatga
     9541 ccaagagttg gtaatcgtca aggctgatag cgtggaagtt caagactatg atggtaacag
     9601 tcgtgaacgt gctagctata ctgcggaact tgacttgtca gatatcggta agggaactta
     9661 tccttactac atgcttaagg aaattgatga gcaaccaact gttatgcgta aactcattca
     9721 agcctacacg gatgatgctg gtcaagtagt gattgatcct gctatcatta aggctgttca
     9781 agacgcagac cgcatctaca tccttgcagc tggaacatct taccatgcag gatttgcttc
     9841 taagaaaatg ttggaagaat tgacagatac accagttgaa cttggcatct catctgagtg
     9901 gggctacggt atgccacttc tcagcaagaa accactcttc atctttatca gccaatctgg
     9961 tgaaacagcg gatagtcgtc aagttttggt caaggctaat gaaatgggaa ttccaagctt
    10021 aacagtgaca aacgttccag gttcaaccct ctcacgtgaa gccaactata ccatgctcct
    10081 tcacgcaggt cctgaaattg ccgtggcatc aactaaagcc tatacagcgc aaatcgcagc
    10141 ccttgccttc cttgcaaaag cagtcggaga agcaaatggt aatgctaaag cgcaagcctt
    10201 tgacctggtt catgaattgt caatcgtagc tcagtctatc gaatcaactc tttcagagaa
    10261 agaaaccatt gaagccaagg ttcgtgaact tcttgaaaca actcgtaacg ccttttacat
    10321 cggacgtggt caagattact acgtagccat ggaagcaagt ctcaaactca aagagatttc
    10381 ttatatccag tgtgaaggtt ttgcggcagg agaactcaag cacggaacca ttgccttgat
    10441 tgaagaagga acacctgtct tggctctctt gtcagatcca gtccttgcca accacactcg
    10501 tggaaatatc caagaggtgg cagcccgtgg tgctaaggtc ctcactatcg cagaagaaaa
    10561 tgttgctaaa gataccgacg atatcgtcct tacgactgta cacccttacc tctcaccaat
    10621 ttcaatggtc gtaccgacgc aattggttgc ttactttgca accctccacc gtggcctcga
    10681 tgtggataaa ccacgtaacc ttgctaaatc agtaacggta gaataagcta aaaaagtcta
    10741 gttatctagg ctttttctaa tgagcaaatt ggttgcttgt actcaagatt cgtgttagaa
    10801 taatttttca aattgaagga cagagataga caaggagaat cacaatggta gaattgggaa
    10861 tttcaacatt tggggaaata acggagcttg aagggactgg acaaacttac agtcatgctg
    10921 aacgcattcg ccagttggtg gcagagattg agctggctga caaggttggt ttggacgtat
    10981 atgggattgg cgagcaccat cgggcggatt ttgcagtatc agccccagag attgtcctgg
    11041 ccgctggggc agtcaatacc aagaaaatcc gtttgaccag tgcagtcagc attctgtcaa
    11101 gcatggatcc gattcgtttg ttccaacagt atgccactat cgatgctttg tcaaatggac
    11161 gatcggagat tatggctgga aggggctctt tcacggaatc tttccctctg tttggttatg
    11221 acttgaaaga ctacgactct ctttttgatg agaaattaga cttgcttcag ttagtcaatg
    11281 aaaagaccaa gttagactgg caaggtcgat tgactcaaac gattgctggt aaagaagttt
    11341 atcctcgtcc agttcaggac aaattgccct tgtggatagc tacaggtggt catgtcgaat
    11401 caacagtgaa gattgctcag gctggtctac cgattgtata tgctattatt ggtggtaatc
    11461 cacgttattt taaaaagttg attcaggctt atcgtgagat tggaagcgaa gcgggccatg
    11521 ctgacaaaga cttgaaagtg ggagcccatt cttgggggtg gattgcggaa gatggtgagc
    11581 aggcggtgaa agattatttc catccgacta agcaagtggt ggatgctatt tccaaagacc
    11641 gtccgcactg gcaggaattg cgttatgagc aatatttgga gcaggtaggg ccaaatggcg
    11701 ccatgtttgt tggcaatcca gatcaggtgg ccgaaaaatt gattcgcatg atagaggatt
    11761 taggattgga ccgcttcatg cttcatctac cgcttggttc tatgcctcat gaccaggttc
    11821 tgagagctat tgaactcttc ggaacacaag ttgcacccaa agtacgagct tattttgcta
    11881 tgaaagaggc ttaataaaaa atcctaatca agtttattag gacaacaaat attgtacaaa
    11941 aattaatccc ccgagctata atgcttgggg gatttttcta taggagggct agtttagttc
    12001 tcttttttat tttttagtaa gaaaccgaga ccaaggagcg caaggaggct gattcctgca
    12061 aataggagtt tgttttcgtt tttgattcct gtatttggaa gttcagcttg tttatctggg
    12121 gttgcaggta tattttcctt gctagagttt tctaccgatt cgtttcgaac cgcttctttt
    12181 acagatgctt cttgtgcttc ttgtgctggt tgttcagctt gtgaatcagc tgtagcttgg
    12241 ctaggtttat tttccgcatc agctactttg gcatctggtt tagtagaatc aggtctagct
    12301 tctggagctg ggtcttgatg tgcagggtga gaagcttcat tttgatgttc aggcttggaa
    12361 ggggttgagt ctggtttctc ttctgcagtt gactcatggc tagttccatt ttgagagact
    12421 cgaagaacag tagctgtaag ggcattcaat ttcaagcctt tttcagtcca ttcaagtcct
    12481 ttcgggttgg caattccgac tgatcctgct tggttttcat ctgccaaaac ttccgcattt
    12541 cttagatgtg caaaggcagt tcccaaatta aattcgcgag ctttttcatc cgcattgaca
    12601 aagactgcgt agatatcacc gtttggagca gtgatttggt agccaatcac tacatcctct
    12661 ttttccacac cattttggcc tgggacagtg atgaggtgga cacggtcttt gatatcttga
    12721 agactcttaa gtcggaaggc atctgtagat tgacgaaggg caatcaaacc tttcatatag
    12781 tcacggctct tgacattttc aggataagct ttaccatctg tagccttagt ccagtcaaac
    12841 ttgttgactg cgtcgctaga atcgtaagaa tcatggatga agtaaggata gtcaaatggg
    12901 ttgccgtcct tatcacgcaa caagtgagat ttgtttggaa ccttatcctc tgctactgga
    12961 gtcttgtagg ctgggtcacg gaattgttta gtacgtccat attcctgacc ggagtggata
    13021 aatggagttc cttgagctgt caagaccatg agatttccaa gtcgtaaacg acggtggatt
    13081 tcagcatagt tctcagcctt gcttgggtct tttttgatag actgggcaat gatgtcaaag
    13141 agggtcaagt tatcatgggc tgcgatgtat tggatgacat ctccagggct gtcagcttca
    13201 aagttagttg gttgagcaat gagattttta aagatggtgt tgacatcacg cttgccacct
    13261 gtgataaagg caggttgacc ttcgtttgga taaccagatt tgaggttgtt acggatgtca
    13321 tctgaaaaga cagcgacagt atcggtatgt ttcatccaat cttggtcagc agccttagta
    13381 ggcatgtttt catcaccggc ataggttctc caaccttcac caagcatgat gaggtttgga
    13441 ttgagggcgc gtgcagcctt gtaagcttct tcgatagaag cggcgtcatg gtctcccatc
    13501 atatcgaagc ggaagccatc cactttgtag gtatcaacta ggtatttgat agagtcaatt
    13561 aggagccgtt tggtcatatg gtgggttgtc cccaagcgtc caccaccaaa gctagttcga
    13621 ggtgtgccat cggcatccat aaagtggtag tagtttggtt ccaaatcttc aaagagatcg
    13681 actttggctg tgtggttata aacgacatct aggatagctc ccataccacg tttgtggatt
    13741 tcgttgatga ggtttttaaa ttctgcgatt cgtttttctg gattcttagg atcgcttgag
    13801 tacataccag tcaaggagaa gtagttttga gggtcatatc cccagttgta gttgctgttg
    13861 cttgaagcgt agtcagacaa gcgttcatgg tttttcaatt cattgacaaa gtagtaagac
    13921 aagactggaa ggagctggat atgggttaca cccaagtctt tgagatagtc tagtttttca
    13981 atgaaggctt caaaagtccc aaatggtttg gtcaagtctt ttgcaatggc aggatctgaa
    14041 gtgaaatcac gcacatgagc ttcgtagata acggcgtctt cacgagtctt gaaattgtga
    14101 atcttaccat aagtcaagtc ttgaggtccg agtttagctg gatctacaaa ggcggcttta
    14161 gccactttat gggcatcgtc aatcttggaa tcgtcgctat tccaagcagc aagagattta
    14221 gcgtaaggat cgagtgcaag aacagtttta ccttgacgct cgatttggta ttgataatag
    14281 tagccagtga aatctgtgat tccgagtttg tttgtgctgt ctagagtttg tttccaagtt
    14341 cctctttccc ctttttcaag agcgacagtt ccaactactt tgtcagggtc attcttgtcg
    14401 tagacaacaa cagaaacctt atcagcactt ggtgaccaaa gggtcaaatc aacttgtttt
    14461 ccttcttctt ttaggtcagc tcccagtttg ccatcatagc tgtatgtctc atctttcagg
    14521 cgccagcttg ttttggtagt gaattggtcg gaattgtagc taacagtata aggatgtttt
    14581 gtgtcagaga aatctccgct gtaggtcact ttcttaccag cttcatcgat tgcaacatcg
    14641 gtaatagtta ccttgtttcc taggtgatta gtgatgttgg agtgtttgag gatatcttct
    14701 tttttagcac cgacaagtgt tgaaaagcta ctttcaatgc tagaagtgcc tacgtgttgg
    14761 gctcctgtca tacggatatc atggacatag tatggatttg tgtaaatcga ttcatcatcg
    14821 tcttttagga aaatttggct atgatttttc aaatctgtga acttataatt ttctttacgg
    14881 attttcacgt cgtctccttg tttgctctca tctagtaata aaaatccaaa ttctcttgcg
    14941 gcttcattaa gaggaatgtc gatatagcgg ccatatttgc ctgtagccgt aaagtctgtt
    15001 ccgtcaggcc attgagcgct acttggattt tttacatctc cccagtacca gagagatttc
    15061 ttgtcatagt tgccatctgt gcggtagtag ttgacgcgaa cagttcctgc aggctgtggc
    15121 tcgtaagaga aaaccttgta atcttggtct aaccaagcct cgttcatttt tggaactagt
    15181 ttttctacag atttatcgcc ggttagattt tttccagctg tattgttgat gaggaagcta
    15241 attttcttgg cttgttctcc ctttaatttg acatctaggt aatagccgta gtcatctttc
    15301 ttggcatcct tgaaggacaa agctccgttt ggccagtttt cagatggttt ttcaacatcg
    15361 tcccaagtcc atagtccttg agcatccttg ttttcttcag gaagtttttt gacatggata
    15421 cggaagtagt tatcttcgat gggctcttct gccttagttt cagttgttac tggactagta
    15481 gaagtagttg gctctgttgc aggtttttct tctgttttct ctagggaagc gtttgcattt
    15541 ctaggtgaag aaatatcact ttcgttctta ccagagccag ctgctgtatc agtaagaggt
    15601 tgggcaaggg tggttgtatt tctgttgttg ggttcgttag tggttggtgt actttcgtcg
    15661 gatgagatag ttggtgtagc catggcaagc aagacaaggc ttgcgccgat aaggacagaa
    15721 ccagttccat ttttgaggga acggatgctg tagaccattt ttttctcagt gtgagatggt
    15781 gtttttctca taatgattct ccaatcaata aatttctata tcagtatagc atgtagtaaa
    15841 acaatatgca agcgttttct ttgatttgtg aaaaaaattc ttacttttga gaagttttct
    15901 gtactatttt atatatcagt ataaatgacg gagataaaaa tgtcacataa gccaagccgt
    15961 attattagga agatgatgtg ttcccagcta tttttattca gaaattgatc tctaacatta
    16021 ccaataaaaa agaaaaatat cttgacaagc aagggaaaat tttgttacaa taatagacgg
    16081 tactttttac ttttggtctc tcaagagtgt acagggacgt gctgacaaat gttgcaaaag
    16141 tacacacaga tgatatctgt caccaagtgt atcatcacca aaaataaaaa aacacaggag
    16201 aatgtagatg cctacaatta accaattggt tcgcaaaccg cgtaaatcaa aagtagaaaa
    16261 atctaaatca ccagctttga acgttggtta caatagtcat aaaaaagttc aaacaaacgt
    16321 ttcttcacca caaaaacgtg gtgttgcaac tcgtgttgga acaatgacac ctaaaaaacc
    16381 taactcagcc cttcgtaaat tcgctcgtgt acgtttgagc aaccttatcg aagttactgc
    16441 ctacatccca ggtatcggac acaacttgca agagcacagc gtggtgcttc ttcgcggtgg
    16501 acgtgtaaaa gaccttccag gggtacgtta ccatatcgtc cgtggtgcac ttgatactgc
    16561 aggtgttaac gatcgtaaac aaggccgttc taaatacggt actaaacgtc caaaagcata
    16621 aggaaagggg ataaagagaa atgagtcgta aaaatagagc tccaaaacgt gacgtattgc
    16681 cagatccgct atacaattca caactagtta ctcgtcttat caaccgcgtt atgcttgatg
    16741 gtaaacgtgg tactgctgct tcaatcgttt acggtgcttt tgagcaaatc aaagaagcta
    16801 ctggcaacga tgcacttgaa gtatttgaaa cagctatgga aaacatcatg cctgtacttg
    16861 aagtacgtgc acgtcgtgtt ggtggttcta actaccaagt cccagttgaa gttcgtccag
    16921 aacgtcgtac aacacttgga cttcgttggt tggtaacaat cgctcgtctt cgtggtgaac
    16981 acacaatgca agaccgtctt gcaaaagaaa tcttggatgc tgctaacaac actggtgcag
    17041 cagttaagaa acgtgaagat actcaccgta tggctgaagc taaccgtgca ttcgcacact
    17101 tccgttggta agataggatg cgaaagcgtt aagaaagtcc cagagaaaat agggaatcga
    17161 agcaggttgc ggttgcaacc aatgagattc atctttttct ccagactttt agcttgagct
    17221 caactaaatc atgatgctag gcacggtaag gatgcaaggt gaaaatagga aactgacgca
    17281 gtattcaacg aatacaagga gttttatctt tttcacgcag catcccgttc cagctcacat
    17341 cggctaacta actttagcct gggttcaaat tagctaaatc gattagtatt agctataact
    17401 cagcttacca tctcgtaagt tgaaaccaac aatagcatga aaacattgag aacgggtagg
    17461 tcctgcctat ccgtttttat taaaatcgtg ttataataga atagaaatca aaaataaata
    17521 ggagaaacaa acctcatggc acgcgaattt tcacttgaaa aaactcgtaa tatcggtatc
    17581 atggctcacg tcgatgccgg taaaacaaca actactgagc gtattcttta ctacactggt
    17641 aaaatccaca aaatcggtga aactcacgaa ggtgcgtcac aaatggactg gatggagcaa
    17701 gagcaagaac gtggtatcac gatcacatct gctgcgacaa cagctcaatg gaacaaccac
    17761 cgcgtaaaca tcatcgacac accaggacac gtggacttca caatcgaagt acaacgttct
    17821 cttcgtgtat tggatggtgc ggttaccgtt cttgactcac aatcaggtgt tgaacctcaa
    17881 actgaaacag tttggcgtca agcaactgag tacggagttc cacgtatcgt atttgccaac
    17941 aaaatggaca aaatcggtgc tgacttcctt tactctgtaa gcacacttca cgatcgtctt
    18001 caagcaaatg cacacccaat ccaattgcca atcggttctg aagatgactt ccgtggtatc
    18061 attgacttga tcaagatgaa agctgaaatc tatactaacg accttggtac ggatatcctt
    18121 gaagaagaca tcccagctga ataccttgac caagctcaag aataccgtga aaaattgatt
    18181 gaagcagttg ctgaaactga cgaagaattg atgatgaaat acctcgaagg tgaagaaatc
    18241 actaacgaag aattgaaagc tggtatccgt aaagcgacta tcaacgttga attcttccca
    18301 gtattgtgtg gttcagcctt caaaaacaaa ggtgttcaat tgatgcttga tgcggttatc
    18361 gactaccttc caagtccact tgacatccca gcaatcaaag gtattaaccc agatacagac
    18421 gctgaagaaa ctcgtccagc atctgacgaa gagccatttg cagctcttgc cttcaagatc
    18481 atgactgacc cattcgtagg tcgtttgaca ttcttccgtg tttactcagg tgttcttcaa
    18541 tcaggttcat acgtattgaa tacttctaaa ggtaaacgtg aacgtatcgg acgtatcctt
    18601 caaatgcacg ctaacagccg tcaagaaatc gacactgttt actcaggtga tatcgctgct
    18661 gccgttggtt tgaaagatac tacaactggt gactcattga cagatgaaaa agctaaaatc
    18721 atccttgagt caatcaacgt tccagaacca gttatccaat tgatggttga gccaaaatct
    18781 aaagctgacc aagacaagat gggtatcgcc cttcaaaaat tggctgaaga agatccaaca
    18841 ttccgcgttg aaacaaacgt tgaaactggt gaaacagtta tctcaggtat gggtgaactt
    18901 caccttgacg tccttgttga tcgtatgcgt cgtgagttca aagttgaagc gaacgtaggt
    18961 gctcctcaag tatcttaccg tgaaacattc cgcgcttcta ctcaagcacg tggattcttc
    19021 aaacgtcagt ctggtggtaa aggtcaattc ggtgatgtgt ggattgaatt tactccaaac
    19081 gaagaaggta aaggattcga attcgaaaac gcaatcgtcg gtggtgtggt tcctcgtgaa
    19141 tttatcccag cggttgaaaa aggtttggta gaatctatgg ctaacggtgt tcttgcaggt
    19201 tacccaatgg ttgacgttaa agctaagctt tatgatggtt catatcacga tgtcgactca
    19261 tctgaaactg ccttcaagat tgcggcttca ctttccctta aagaagctgc taaatcagca
    19321 caaccagcta tccttgaacc aatgatgctt gtaacaatca ctgttccaga agaaaacctt
    19381 ggtgatgtta tgggtcacgt aactgctcgt cgtggacgtg tagatggtat ggaagcacac
    19441 ggtaacagcc aaatcgttcg tgcttacgtt ccacttgctg aaatgttcgg ttacgcaaca
    19501 gttcttcgtt ctgcatctca aggacgtggt acattcatga tggtatttga ccactacgaa
    19561 gatgtaccta agtcagtaca agaagaaatt attaagaaaa ataaaggtga agactaatcc
    19621 gtcctcactc tagaaggaag tcacttagtg gcttcctttt gtctttagaa aatacctcta
    19681 aatatggtaa aatagtagaa gaataatgtg aggaaaatga atgtcaaata gttttgaaat
    19741 tttgatgaat caattgggga tgcctgctga aatgagacag gctcctgctt tagcacaggc
    19801 caatattgag cgagttgtgg ttcataaaat tagtaaggta tgggagtttc atttcgtatt
    19861 ttctaatatt ttaccgattg aaatcttttt agaattaaag aaaggtttga gcgaagaatt
    19921 ttctaagaca ggcaataaag ctgtttttga aattaaggct cggtctcaag aattttcaaa
    19981 tcagctcttg cagtcctact atagggaggc tttctctgaa ggtccatgtg ctagtcaagg
    20041 ttttaagtcc ctttatcaaa atttgcaagt tcgtgctgag ggtaatcagc tatttattga
    20101 aggatctgaa gcgattgata aggaacattt taagaagaat catcttccta atttagccaa
    20161 acaacttgaa aagtttggtt ttccaacttt taactgtcaa gttgagaaga atgatgtcct
    20221 gacccaagag caggaagagg cctttcatgc tgaaaatgag cagattgttc aagctgccaa
    20281 tgaggaagcg ctccgtgcta tggaacaact ggagcagatg gcacctcctc cagcggaaga
    20341 gaaaccagtc tttgattttc aagcgaaaaa agctgcagct aaacccaagc tggataaggc
    20401 ggagattact cctatgatcg aagtgacgac agaggaaaat cgtctggtat ttgaaggggt
    20461 tgtttttgat gtggagcaaa aagtgactag aacaggtcgt gttttaatca actttaaaat
    20521 gacggactat acttcaagtt tttctatgca aaagtgggtt aaaaacgagg aagaggccca
    20581 gaagtttgac ctcatcaaga agaattcttg gctccgagtt cgagggaatg tggagatgaa
    20641 taacttcaca cgcgatttga ctatgaacgt acaggatctg caggaagttg ttcactatga
    20701 gcggaaggat ttgatgccag aaggtgagcg tcgggttgag tttcatgctc atactaacat
    20761 gtcgactatg gatgctttgc cagaggtcga agagattgtt gcaacagctg ctaagtgggg
    20821 acacaaggcg gttgctatca cagaccatgg gaatgtccag tcctttccac atggctataa
    20881 ggcggctaag aaagcgggaa tccagctgat ctatgggata gaagccaata tcgtggagga
    20941 ccgtgtccct atcgtctata acgaagtgga gatggactta tcagaagcaa cctacgtggt
    21001 ctttgacgtg gaaacgacgg gactttcagc tatctataat gacttgattc aggttgcggc
    21061 ctctaagatg tacaagggga atgttattgc tgaatttgat gaatttatca atcctgggca
    21121 tcccttgtca gcttttacta cagagttaac tggaattaca gatgatcatg tcaaaaatgc
    21181 caaaccacta gaacaagttt tgcaagaatt ccaagaattt tgcaaggata cggtcctagt
    21241 tgcccacaat gctgcctttg acgttggctt tatgaatgct aattatgagc gtcatggtct
    21301 tccaaagatt agtcagccag ttattgatac gctggagttt gctagaaacc tctatcctga
    21361 gtataaacgc catggtttgg ggcctttgac caagcgtttt ggtgtggcct tggaacatca
    21421 ccacatggcc aactacgatg cggaagcgac tggtcgtctg cttttcatct ttatcaaaga
    21481 ggtagcagaa aaacatggtg tgaccgattt agctagactc aacattgatc taatcagtcc
    21541 agattcttac aaaaaagctc ggatcaagca tgcgaccatc tatgtcaaga atcaggtagg
    21601 tctaaaaaat atctttaagc tggtttcctt gtctaatacc aagtattttg aaggagtgcc
    21661 acggattccg agaacggttc tagatgccca tcgagagggc ttgattttag gttcagcctg
    21721 ttcagagggt gaagtttttg acgtggtcgt ttctcaaggt gtggatgcgg cggttgaggt
    21781 ggccaagtat tatgacttta tcgaggtcat gccaccggct atctatgcgc ccttgattgc
    21841 taaagagcag gtcaaggata tggaggaact ccagaccatt atcaagagtt tgatagaggt
    21901 tggagaccgc cttggcaagc ctgttttggc tacgggaaat gttcactata tcgaaccgga
    21961 agaagagatt tatcgtgaaa ttatcgtccg tagtttggga cagggtgcga tgattaaccg
    22021 aactatcggt catggtgaac atgcccaacc agcaccactt ccaaaggctc attttcgaac
    22081 gactaatgag atgttggatg aatttgcctt tttgggagag gaactggctc gtaaactggt
    22141 tattgaaaac accaatgcct tggcagaaat atttgaaccc gttgaagtcg ttaagggtga
    22201 cttgtatacg cctttcatcg acaaggctga agaaacagtt gctgagttga cctataagaa
    22261 agcttttgag atttatggaa atccgctgcc agatattgtt gatttgcgga ttgaaaaaga
    22321 attaacatcc atactgggga atggatttgc tgtgatttat ctggcatcgc agatgctggt
    22381 gcaacgttct aatgaacggg gttatttggt tggttctcgt gggtctgtcg gatctagttt
    22441 cgttgcgacc atgattggaa ttacggaggt caatcctctc tctcctcact atgtctgtgg
    22501 tcagtgtcag tacagtgagt ttatcacaga tggttcgtac ggttcaggat ttgatatgcc
    22561 ccataaggac tgtccaaact gtggtcacaa actcagtaaa aacggacagg atattccgtt
    22621 tgagaccttc cttggttttg atggggataa ggttcctgat attgacttga acttctcggg
    22681 agaagatcag cctagcgccc acttggatgt gcgtgatatc tttggtgaag aatatgcctt
    22741 ccgtgcggga acagttggta cggtagctgc caagactgcc tatggatttg tcaagggtta
    22801 cgagcgagat tatggcaagt tttatcgtga tgcagaagta gaacgcctcg ctcaaggagc
    22861 ggcgggtgtc aagcggacaa caggccaaca cccgggggga atcgttgtta ttccgaacta
    22921 catggatgtc tacgatttta cgcctgtcca gtatccagca gatgatgtca cggctgaatg
    22981 gcagaccact cactttaact tccacgatat cgatgagaac gtcctcaaac tcgatgtact
    23041 gggacatgat gatccgacca tgattcgaaa acttcaggat ttgtctggta ttgaccctaa
    23101 taaaattcct atggatgacg aaggcgtgat ggcactcttt tctgggactg atgtgctagg
    23161 ggtaacacct gaacaaattg gaacgcctac gggtatgttg gggattccag agtttggaac
    23221 aaatttcgta cgtggaatgg tagacgaaac ccatccgaca acctttgcgg aattgcttca
    23281 gctgtctggt ctgtcccacg gtactgatgt ttggttgggg aatgctcagg atctgattaa
    23341 gcaaggaata gcggacctat cgactgttat cggttgtcgg gacgacatca tggtttacct
    23401 catgcatgcg ggtctggaac ctaagatggc ctttaccatt atggaacggg tacgtaaggg
    23461 tttgtggcta aagatttcag aagaggagag aaatggctat atcgaagcaa tgaaggctaa
    23521 taaggtgcca gagtggtata tcgaatcctg tgggaaaatt aagtacatgt tccctaaggc
    23581 ccatgcggca gcctacgtta tgatggcctt gcgtgtagct tacttcaagg ttcaccatcc
    23641 tatttattac tactgtgctt acttctccat tcgtgctaag gcttttgata tcaagaccat
    23701 gggtgcgggc ttggaggtca tcaagcgcag aatggaagaa atctctgaaa aacggaagaa
    23761 caatgaagcc tctaatgtgg aaatcgatct ctatacaact cttgagattg tcaatgagat
    23821 gtgggaacga ggtttcaagt ttggtaaatt agatctctac cgtagtcagg cgacagagtt
    23881 cctcatcgac ggggataccc ttatcccacc atttgtagca atggatggtc tgggagagaa
    23941 cgttgccaag caactggtgc gggcgcgtga agagggagaa ttcctctcta aaacagaact
    24001 acgcaagcgt ggtggactct catcaacctt ggttgaaaag atggatgaga tgggtattct
    24061 tggaaatatg ccagaggata accagttgag tttgtttgat gagttgtttt aaaaaattgc
    24121 ttaataatct attaaaagag gctaacgtat atccaataga tttacattag ctttcttttt
    24181 tgttaaaata gtctatggaa agagggtgag agtatgtcaa agatgagtat aagcatccgt
    24241 ctggatagtg aggttaagga gcaggcccaa caggtgttta gtaatctggg aatggatatg
    24301 acaacagcta ttaatatttt ccttcgtcag gcaattcaat atcagggatt accttttgat
    24361 gttagactag acgaaaatcg gaagttgctc caagcgttaa cggatttaga ccaaaatcgt
    24421 aatatgagcc agtcttttga atcagtctca gatttgatgg aggacttacg tgcttaagat
    24481 tcgttatcat aaacagttta aaaaagattt taagttggct atgaagcgtg gtttgaaggc
    24541 agaattatta gaagaagttt tgaattttct ggttcaagaa aaagaacatc ctgccagata
    24601 tcgtgatcat tcattgacgg catccaagca ttttcaagga gttcgtgaat gccataccca
    24661 gccagattgg cttttggttt ataaagtaga caagtcggaa ttgattttaa atttgctgag
    24721 gacaggcagt cacagtgatt tattttaatc tattttaagg gggttctcat gaaactaaga
    24781 atatttgcgg aagataagcc ggctaagaag gtatttgaat atcaattaga acttgctgat
    24841 cgtacaattc ttctatcgac agcactcttg tcaggtgcta ttgctttagc aggaatcttt
    24901 tctgctttga aagaaaaata aaaatagaaa agagaaaaca gaatggtttt accaaatttt
    24961 aaagaaaatc tagaaaaata tgcgaaattg ttggttgcga acggaattaa cgtgcaacct
    25021 ggtcacactt tggctctctc tattgatgtg gagcaacgtg aattggcaca tctaatcgtg
    25081 aaagaagctt atgccttggg tgcgcatgag gtcatcgttc agtggacaga tgatgtgatt
    25141 aaccgtgaga aattcctcca tgccccgatg gagcgtttgg acaatgtgcc agaatacaag
    25201 attgctgaga tgaactatct tttggagaac aaggctagcc gtcttggggt tcgttcatct
    25261 gatccaggtg ccttgaacgg agtggacgct gacaagcttt cagcttctgc taaagctatg
    25321 ggacttgcca tgaagcctat gcgtatcgca actcaatcta acaaggttag ctggactgta
    25381 gcagccgctg ctggacttga gtgggctaag aaagtcttcc caaatgctgc gagcgacgaa
    25441 gaagcagttg atttcctttg ggatcaaatc ttcaaaactt gtcgtgtcta cgaagcagat
    25501 cctgttaagg cttgggagga acatgcagcc attctcaaga gcaaggccga tatgcttaat
    25561 aaggagcaat tttcagccct tcactacaca gcgccaggaa cagatttaac acttggtttg
    25621 ccaaagaacc acgtttggga atcagctggt gctgtcaatg cacagggcga agaattcttg
    25681 ccaaatatgc cgacagaaga ggtcttcaca gcgcctgact tccgtcgtgc agatggttat
    25741 gtcacttcta caaaaccgct tagctacaac ggaaatatca ttgaaggcat taaggtgacc
    25801 tttaaggatg gacaaatcgt agatatcact gctgagaagg gtgatcaggt tatgaaagac
    25861 cttgtctttg aaaatgcggg tgcgcgtgcc ttgggtgaat gtgccttggt accagatcca
    25921 agtccaattt ctcagtcagg cattaccttt tttaacaccc ttttcgatga aaatgcgtca
    25981 aaccacttgg ctatcggtgc agcctatgcg actagcgttg ttgatggagc ggagatgagc
    26041 gaagaggagc ttgaagctgc agggcttaac cgttcagatg ttcacgtaga ctttatgatt
    26101 ggttctaacc aaatggatat cgatggtatt cgtgaggatg gaacgcgggt acctcttttc
    26161 cgtaatggga attgggcaaa ttaaggagat aatatgttag gaagtatgtt cgttggtctc
    26221 ctagtgggat ttttagcagg tgctatgacc aatcgtggag agcgaatggg atgttttgga
    26281 aaaatgtttc tcggttggat cggagccttt ctaggtcact tgctctttgg aacttggggg
    26341 ccagttttat caggaacagc tattatccca gcgattttag gagccatgat tgttttagct
    26401 attttttgga gacgaggaag ttaatttcct aaatctgata ctcaatgaaa atcaaagagc
    26461 aaactaggaa actagccgca ggttgctcaa agcactgctt tgaggttgca gatagaactg
    26521 acgaagtcag ctaaaaacac tgttttgagg ttgcagatag aactgacgaa gtcagctaaa
    26581 aacactgttt tgaggttgca gatagaactg acgaagtcag taacatatat acggcaaggc
    26641 gacgttgacg cggtttgaag agatttttga agagtatgaa acgaaaagga ggttggtcat
    26701 tgtactagcc tccttttgat atgatataat agttctatga gattagataa atttttagtt
    26761 gcctgtgcgg tgggaagtcg gactgaggtc aaaaacttgc tcaaggctgg gcgcgtgact
    26821 gtaaatggta aaaaagaaaa atcagctaaa ttgcagattg atgaaaaaat agatgagatt
    26881 cgctttgatg ggcaagtgtt ggagtatgaa gagtttgtct actacatgat gaacaagccc
    26941 aaaggagtta tctcagcgac tgaggatccc aagcacagaa ccgttctgga cttgttggat
    27001 gacttggcgc ggagtaagga agtttttcca gtaggacgct tggatattga cacgcatggt
    27061 cttttgctct tgaccaataa tggtcagctg gctcatgttc ttctttcgcc caagcgtcat
    27121 gtggacaaga cttatcaggc tcaggtcaag ggaattatga cccaagaaga tgtggagaca
    27181 tttatcaagg gcattccgct caaagacttt acctgtcagc ccgctagact ggagattctt
    27241 tccacagatg cagaaaagaa tcagagccaa atccgtgtga ccattgcaga agggaagttt
    27301 catcagatca agcgtatggt gggctactgt ggcaaggaag tggtggactt gcaacgattg
    27361 actatgggaa ctttagtatt agatgagaac ctggaacgag gggaatggcg tcgcttgacc
    27421 aaggaagaat tagaaattct ccgcgctaat attatttaat ttagtttaat actcaatgaa
    27481 aatcaaagag caaactagga aactagccgc aggctgtact tgagtacggc aaggcgacgt
    27541 tgacgtggtt tgaagagatt ttcgaagagt ataaattcga aaaaggtgag ggacaatgtc
    27601 ctcgcctttt atgtttttta gttgcttcct ttgtgaaaag agttataata gactgtagaa
    27661 taaaaggagg aatctatgaa cgcgattcaa gaatcattta ctgataaact atttgccaac
    27721 tatgaagcaa atgtaaaata ccaagcgatt gaaaatgctg ccagccacaa cggaattttt
    27781 gcagctctgg aatgtcgcca aagccatgta gacaacacac ctgttttctc attggattta
    27841 accaaggaca aggtcactaa ccagaaagcg tctggtcgtt gctggatgtt tgcggctctc
    27901 aacaccttcc gccacaaact catctcgcaa tacaaattgg agaactttga gttgtcacaa
    27961 gcccacactt tcttctggga taagtatgag aaatcaaact ggttcttgga gcaagtcatt
    28021 gcgacttcag accaagaatt gactagccgc aaggttagct tcttactcca aacacctcaa
    28081 caagatggcg gtcaatggga tatggtcgtt tccctctttg aaaaatacgg tgtcgtgcct
    28141 aagtcagttt atcctgagtc tgtttcatct agcagcagtc gtgagctaaa tgcgatcctt
    28201 aataaattgc ttcgtcaaga tgctcaaatc ttgcgtgatt tgcttgtttc tggtgcagat
    28261 caagcgactg ttcaagctaa gaaagaagac ctcttgcaag aaatctttaa ctttcttgct
    28321 atgtcattag gacttccacc acgcaagttt gactttgctt atcgcgataa agataacaac
    28381 tataaaagtg aaaaaggaat cacaccacaa gagttttaca agaaatatgt caatcttcct
    28441 ttagaagact acgtttctgt tatcaatgct ccaactgctg ataaacctta cggaaaatct
    28501 tacacagttg agatgttggg gaatgtggtt ggtagccgtg cagttcgcta catcaacgtt
    28561 ccaatggagc gcttgaaaga attggcgatt gcccaaatgc aagcaggtga gactgtttgg
    28621 tttggttctg atgtcggcca gctcagcaac cgtaaggctg gcatccttgc gacagatgtt
    28681 tatgactttg aatcaagcat ggacattaaa cttactcaag acaaggctgg acgtttggac
    28741 tatagcgaaa gcttgatgac ccacgccatg gtcttgacag gtgttgactt ggacgaaaat
    28801 ggtaaatcaa ccaagtggaa ggttgaaaac tcatggggag acaaggtcgg tacagatggt
    28861 tactttgttg cctcagacgc ttggatggac gaatacacat accaaatcgt tgttcgtaag
    28921 gaattgctga cagcagaaga acaagctgcc tatggagcag aaccaatcgt acttgcacca
    28981 tgggatccaa tgggagcctt ggctgaataa aagcatagaa aaaaggaatc agattttaga
    29041 acctggttcc tttttagttg cttgattaca tgatgtgaag aacatgtgcc acaataccca
    29101 ctgcgaagag ggcaaggata atagtgattg gagatacttt tttcttaagt aagtacatgc
    29161 aaagtaaagt aaggagtagt cctgataatc caggaatcaa catatccaag ttttgttgga
    29221 aagtagtaac tttttcagga gtttgagaca atccttgtcc tacttgtgcg aatgcttctt
    29281 ggataccttt agacccttct ggcaatttat cccaatggat ataagccttt tcatctagtt
    29341 gaactttaga aacatcgaaa gcaaatttaa tatttaccca gcgttgaaca aggacagcaa
    29401 gaatgaacat cccaaggata gaagctcctt tagtgatatc ttgaaggata ccaccagaca
    29461 tatctttagt gatttctgat ccagccttgt atccaatctc ttgaacatac cacaagaatg
    29521 acatacgaat caagttccat gcaacaaaga agaggagtgg ccccaagata ttgccagtaa
    29581 gggcaagtga agcaccgaga gatccaagga ttgggcgtac tgtaaaccag aatactgggt
    29641 caccgatacc agcaagaggt cccatcatac cgattttaac cccttggata gcagcgtcat
    29701 cgatttccac accgttagca cgttcttctt caagcgcaag agtaaccccc atgactggag
    29761 cagctacgta tggatgagtg ttgaagaact caaggtgacg ctcaagagca gcgatttgat
    29821 cttctttttt agtatagagt tttttgatag ctggaatgag tgtataagcc cagcccaagt
    29881 tttgcatccg ttcaaagttc caagaccctt gtaagaaggt tgaacgccac caaacttttt
    29941 tacgatctga tttagttaat tgaagttttt cagtcatgat gttttcagtc ctttcttatc
    30001 ttagtagtct tctaggatat cgccgattgg gtcgttagaa gttgcggctc ctccgccacc
    30061 atttccacca gttttagaaa ggtgaaggta gataagagcg atagcaacac cgatagcacc
    30121 gaatccgatt agagtaatat ctgacacagc agcaagaacg aaaccaagag cgaagaatgg
    30181 ccatacttca cgagttgcca tcatgttgat aaccatggcg taaccaacgg caacgaccat
    30241 accaccaccg atagccatac catctttgag ccagtctggc atggcactaa ggatactttg
    30301 tacagtttca gttggtacca taaggagaag agctgcagga agcgcgatac gaagtccttg
    30361 gaaaagtagc gcaatgaaat gagcacgctc cacagcgccg aagtcacctt ttttagcggc
    30421 agcatcagca gtgtgaacca atccaactga aattgtacga acaatcatag ttaagaatag
    30481 accagctacg gcaagaggga tagcaaccgc ttgggcaaca ccgataccag tcttggtaaa
    30541 gtcaccacca agaaccatga taatggcagc agcgacagaa gcaagtgcag catcaggagc
    30601 gatagcagca ccgatatttg accaaccaag ggcaatcatt tgaagcgatc caccgaggat
    30661 aatccctgct tccaagtgac ctgttacaag cccaataagg gtacaggcta caagtggttg
    30721 gtgaaattgg aactggtcga ggatgccttc aagacctgca aagaaggcta caacgactac
    30781 taaaaccata gaaataatag acatgtttaa aatcctttca taaataatgg cttatttgac
    30841 attggctttg ttaatcaagt caaacaaatc ttttttagaa tcatttggta ctttacggac
    30901 atcaaattca acacccaagt cacgcatttt ttcaaatgta gcaacatctt ctttgtccat
    30961 agacaaaacg gtattgacca atgttttacc tgttgagtga gccatagaac caacgttaag
    31021 agtcttgatt ggcacgccgc cttcgatggc acgaagggca tcttgaggtg tttcaaacaa
    31081 gataagggca tgtgtttctc caaaacgtgg gtcttttgaa atctcaatca gtttttgaat
    31141 tggaaccacg ttagccttga cattacctgg agctgcttgt ttaatcaatt ctttacgaag
    31201 gtcgtcttta gccacgttat ctgaagcaac gatgatacga tttgcttttg aatctggagt
    31261 ccaagcagtt gcaacctgac cgtgaagtag acgtgtgtca agacgggcaa gattgatttt
    31321 caatttaccg tctccgataa cagttccttc tgggatagca gtttgggcaa ctggagcagc
    31381 tgcagcgctt gcaacttctt caactggatt tagctcttct ggaagagctt tgatgccatc
    31441 tttggcttct ttaatgatat tagcagcgac tttttctaca cctgcagcag cgtccatgag
    31501 gcgctctgtg taggcttgaa tcaacatcgg taagttaagt cctgtgatga tggcaaactt
    31561 acgctcagga ttttctccca tcacgcgact agcttggtta aatggagaac cactccaaag
    31621 gtcagccaaa actagaacct catcttctgc gtcaaatgca gcaacagcgt tattaaactt
    31681 agcgtataga tcatcaggac cttcatttgg cataaaggtc acaacttgaa ccttttcttg
    31741 ttcaccaaag atcatagatc ctgactgatg aatacccgca gcaaattcgc cgtggctcgc
    31801 aataatgatt ccgatactca ttattgtcat tcctcctttt ttgttttagt tttcatttaa
    31861 gaaaacttta agactgttta agtataaacc gttttcataa aaaaagcaac tatttatcat
    31921 aaaaaattaa aaaattttta tttttatatg ttgatatgtt gagtttttaa ggagtaatct
    31981 tgaattctta tttaaaaatt taatataaaa aagttagaag aagcttccaa cttttaaaat
    32041 aggtttctat tagattagtg ggtgaagtcg agtaccatac gtccttggat ttgacctttt
    32101 tccatttcgt cgaaaatggc aacagcatct tctactggac gtttttgaac aactggggct
    32161 accagacctt ctgcaccaaa ttggaaggct tcttctaagt ctttacgagt tccgacaaga
    32221 gaaccgatga cttggattcc atcgaggact gttttaacga tactgagttc catcatttca
    32281 gaaggaagac caacagcgac gacgcgacca ccagcacgaa cggagtcaac agcctggttg
    32341 aaggcaactt tagacacagc agttacgaca gctgaatgag ctcctccatc agtttttcct
    32401 ttaatgagtc cagctacatc ttcaacttcg aggccgttaa tcacaatgtc agcgcctact
    32461 tcttttgcaa gggcaagttt gtcattgttg atatcgactg cgataacatg agcattgaat
    32521 acttttttag cgtattgaac agcgaggtta ccaagtccac cagcaccgta aagaacaacc
    32581 cattggcctg gttcaacttt tgcttctttg atagctttat aggttgttac tccagcacat
    32641 gtgatagaag aagcttgggc tggatcaagt ccgtcaggaa ccttgacagc atagtcagca
    32701 gttacgatac attgttcagc cataccaccg tctactgagt agccagcatt tttcactgta
    32761 cggcaaaggg tttcgcgacc agttgtacag tattcgcaag tgccacatcc ttcaaagaac
    32821 caagcaacgc tgacgcggtc accgacttta aggcttttca catctggagc aatctcttta
    32881 acgataccga taccttcgtg cccaagaaca cgtcctggga cttgaccaaa gtcaccatga
    32941 gcaacgtgga gatcggtgtg gcaaacgcca cagtattcaa cttctacaag tgcttcccca
    33001 gtttcaagtg gacggagtac tttttcttca atagcaacac cagtgctttc tggatttaca
    33061 acaacagctt tcatagctat cctccttgat attgaccgag aacagggaca acgggactgg
    33121 attgattttt tgtcaacaga gtcgagtgtg acgagctctc ttgtatttat atgtgaagta
    33181 tatcacaaaa gaaagccttt tccaaggttt tggagacaaa aagtagaaca atcgattaac
    33241 tggttgatga gactgtgaaa agagcacaaa gaccagcttg caagctggtc atactcttcg
    33301 aaaatctctt caaaccacgt cagcttcatc tacaacctca aagcagtgct ttgagcaacc
    33361 tacggctagc ttcctagttt gctctttgat tctcattgag tatcagtata ggttataaga
    33421 ataagtcttg tagggttttg gcaaccccat cttggtcgtt ggtaagggaa atttgctcat
    33481 ctgcataagg gagtagctct ggattggcat ttttcatggc ataacccttc ccagcaaaag
    33541 cgagcatttc ggtatcattg tgttcatctc caaaggcaat caaatctttt ttgtcacgat
    33601 tcattatctt gagcaagtag tccaaagcaa aggccttgtt gacacctttt ggggtacatt
    33661 caaggatatt gagcggacct ccccaggtat tgatagacag ttgatgctgg tagaaggcgt
    33721 tcatttcttt tgccagagca tatttgtcac tggctctagt ctgtaacaga atacagttag
    33781 gatccttggt tactaactca ggctgaaatt gattttcagg ctggaaagct tctacaccaa
    33841 atagtttggg attagcaatt tcttcattgg gatttgtaat gtaaaatttt ttacggtatt
    33901 ctccagcgat aaaatcggct tgaatgtcct ctgaacgtcg aaccatgtct agcagatatt
    33961 ttttgtctac agttaaacac ttttcaaaat cccaaacttg gtctggtaaa tgagtaaggg
    34021 agccgttgaa attaatcata ggagtgttta agcctagttc atggtaaaaa tcttttgaca
    34081 tgcggtaagg gcgacccgtc gtaataataa tctgatggcc tttttcagca acttttttaa
    34141 tggtttcttt ggtaaagtca gaaatttgac tgtctgagtt gagcagggtt ccatccaggt
    34201 caactgcaat aatttttttc gtcataggga cctttctagt gtcttttcag ataagatgtc
    34261 tactattata acagaaagca ggcagaaaag cagattcgaa acaaaaaaag aaatttcatc
    34321 aaattcttaa ataaatcaaa caaagatatt gaaaaagtga atgataaatg atataattct
    34381 attattgttc gtaaaaatta aaaggagatt gataatggac aaattattta aactaaaaga
    34441 gaacggtaca gacgttcgta cagaggttct cgctggttta acaactttct ttgcaatgag
    34501 ctatattctc tttgtaaacc cacaaattct ttcgcaaaca ggaatgcctg ctcagggtgt
    34561 attcctcgca acgattattg gtgcagttgc tggtaccctg atgatggcct tctacgctaa
    34621 cttaccttat gcccaagcgc caggtatggg actcaatgcc ttctttacct ttacagttgt
    34681 attcggactt ggttattctt ggcaagaagc cctagctatg gtcttcatct gtggaattat
    34741 ctcattgatt attaccttga caaatgttcg taaaatgatc attgaatcga ttcccaatgc
    34801 tcttcgctca gctatttcag ctggtatcgg tgtcttcctt gcctatgtag ggattaagaa
    34861 tgctggactt ttgaaattta cgattgatcc aggtaactat actgttgtag gagaaggggc
    34921 tgacaaagct caagcaacga ttgcagcaaa ctcttcagca gttccaggat tggtcagctt
    34981 taataatcca gctgttttgg tggctcttgc aggactcgct atcacaattt tctttgtcat
    35041 caaaggaatt aaagggggaa ttctcctctc tatcttgaca acaactgttc ttgctattgc
    35101 agttggtttg gtagatttgt caagcatcga ttttgctaat aaccatgttg gtgcagcttt
    35161 taaagatttg aagacaatct ttggtgcagc tcttggttca gaaggtttgg gagctttgat
    35221 ttcagataca gctcgcttgc ctgaaactct gatggctatt cttgcctttt cattgacaga
    35281 tatttttgat acaattggta ccttgatcgg tacaggtgaa aaagttggta tcgtagcgac
    35341 aaatggtgaa aatcatcaat cagctaagtt agacaaggct ctttactctg acttgattgg
    35401 tacttcaatt ggtgccatcg caggtacttc gaacgtaacg acttatgttg agtctgctgc
    35461 tggtatcggt gcaggtggac gtactggttt gacagccttg gtcgtggcaa tctgttttgc
    35521 gatctcaagc ttctttagcc cacttctagc gattgtacca acagccgcta cagctccaat
    35581 cttgattatc gttgggatta tgatgttggc tagcttgaaa aatatccatt gggacgatat
    35641 gtctgaagcg gttcctgctt tcttcacatc tatctttatg ggattcagct actctatcac
    35701 tcaagggatt gcagttggtt tcttgactta cactttgact aagcttgtca aaggtcaagc
    35761 taaagatgtt catgtcatga tttggatttt ggatgctttg tttatcctta actacatcag
    35821 tatggcacta taaatgctat aatataaaaa ggggttctcc cctttttata ttatagttac
    35881 ttgaaaattg cttcaaacta ggtaggtgat tttgattgaa tggtaggagt ataattgatg
    35941 ataaaaaaga atatttggga tgaagtatta aacaggggga agtggttaat aatctttcta
    36001 gtagggcttt tgttttctca gtttccttta gccctagcaa cttttttaac tagcagaaaa
    36061 tttccactgc ttcaaacggg actattagta ggggccttat ctcttgtggt tttaactgta
    36121 tttataatag gtgctaggaa aacacagttg gctagttttg gtttatcgtt tttcaaggct
    36181 aaggatttgg ctagattggc cttaagctat ctagtgattc ttgcctttaa tattcttggt
    36241 gtggtcttac tgcatttgat gaatgaaaca acaaccagca atcaatcaaa tattaatgat
    36301 ttggtccaga atagttcctt aatttccagt tttttcttac tgcttctaat agctcctatt
    36361 tgtgaggaga ttttatgtcg aggagtgatt cctaaaaaac tctttcgtgg taaagaaaat
    36421 gtgggttata tagtcggtgc tattatcttt gccttgcttc atttaccgac aaatctacct
    36481 tctcttctta tctatggagg aatgtcaact gtgctaactt ggacagttta taaaacccaa
    36541 cgtttggaaa tgtctatctt acttcacatg attgttaatg gaattttttt ctgtttgttg
    36601 gctctcgtgg tgattttgag tcggacatta gggatctctg tttaagattt ttgacaattg
    36661 cttacttgtt tctgctggga aaaagatgaa tgcaatcgtg tccatctttt tctttttatg
    36721 gtaaaataga gaaataatat gatgaaaagc cttgagggag tgaccgatat gtcaactaaa
    36781 gccaatcatg caaagacagc tatttgcgga attatcaatg taaccccaga ctccttttcg
    36841 gatggtggtc aattttttgc tcttgagcag gcactccagc aggctcgtaa attgatagca
    36901 gaaggggcta gcatgctaga tatcggcgga gaatcgactc ggccgggaag tagctatagc
    36961 tatgttgaga tagaagagga aatccagcgt gttgttccag ttattaaggc tattcgcaag
    37021 gaaagtgatg tcctcatctc tattgatact tggaagagcc aggtagcaga ggctgctttg
    37081 gctgctggtg ccaatctagt caacgatatc actggtctca tgggcgatga aaaaatggct
    37141 cgtgtggttg ctaaggctgg agcgaaagtg gttatcatgt ttaatccagt catggctcgt
    37201 cctcagcatc ctagctcgct catctttcct cattttggct ttggtcaagc ttttacagag
    37261 gaagagttag ctgactttga aacattgcca atcgaagact tgatggatgc tttctttgaa
    37321 cgagcactag cgagagcaaa tcaagctgga attgcacaag aaaatatcct gttggatctg
    37381 ggaatcggct ttggtctgac caagaaagaa aatctgcttc ttttacggga cctggataaa
    37441 ctacatcaga agggctatcc aatctttctt ggagtgtcgc gcaagcggtt tgtcatcaat
    37501 atccttgaag aaagtggttt tgaagtcaat cctgagacag aacttggttt ccgaaatcgg
    37561 gacacggctt cggctcatgt aactagtatc gctgcgagac agggtgtaga agtggtgcgc
    37621 gtgcatgacg tagctagtca caggatggca gttgaaattg cctctgccat tcgtctggct
    37681 gatgaagcgg aaaatttaga tttaaaacaa tataaataag atgaaagaaa ttgaaaataa
    37741 tcagtggatt gctaactacc ggacggatca accgcatttt ggcttggaac gaatggtgga
    37801 actgttagcc ttgcgtggaa atccccatct caaactcaag gtcatccata tcggagggac
    37861 taacggcaag ggttcgacca ttgctttttt gaagaatatg ctggaaaagc tagggctgag
    37921 agttggggtg ttcagctcgc cctatctcat tcattacaca gaccagatta gcatcaatgg
    37981 ggaatccatt ccagaggcta ggctagaagc cctcatggca gactatcagt ctttgctgga
    38041 gggagaagcg gtcgccaatt tacagagtac aaccgagttt gagattatca cagccctggc
    38101 ctatgactac tttgccgcag agcaagtaga agtggctatc atggaagtcg gtatgggtgg
    38161 acttttagat agtaccaatg tctgtcagcc cattttgaca ggaattacga ctattggctt
    38221 ggaccatgta gccctacttg gtgatacctt ggaagccata gcagagcaga aagctggtat
    38281 tatcaaacaa ggtattccct tggtgacagg tcgtatcgct ccagaagctt tggctgtgat
    38341 tgaccgcatt gcggaaggga aagatgcgcc gagacttgcc tacgggacag attatcaggt
    38401 cagccatcaa aagagtgtgg ttacaggcga agtctttgac tatacaagtg ttgtcaggca
    38461 aggtcgcttc caaactggcc tgcttggctt gcaccagata gagaatgctg ggatggccat
    38521 agctttactt gatacttttt gtcaagaaca tggtcgagag ctagcaagca atgacctgct
    38581 tggtcaagcc ttggaagaaa caagttggcc agggcgtttg gaagttgtgt cgagagaccc
    38641 cctgatgatt ttggatggag cccacaatcc ccatgcgatc aaggccttat tggcaacctt
    38701 gcaagaacgc tttgcggatt atcgtaagga aatcctcttt acttgcatca aaaccaaggc
    38761 cttagaggat atgttggact tgctgggggc catgccagat accgagctaa ctctaacaca
    38821 ttttgacgat agtcgggcga ctgatgaaag cgtgctggaa gaggcggcta agtctagaaa
    38881 tctcaactac caaggttggc aggattttct agagcagaaa ttgacagata aaaaagaaga
    38941 gaaaaaaaca gttaggattg tcacgggttc cttgtatttc ttgagccaag tgagagccta
    39001 tctgatggag aggaaaaacg aaaatggata cacaaaagat tgaagcagct gtaaaaatga
    39061 ttatcgaggc tgtcggagag gacgctaacc gtgagggctt gcaggaaacg cctgctcgag
    39121 tggcccgtat gtaccaagag attttttcag gtcttggtca aacagcggag gaacatttgt
    39181 caaaatcctt tgagattatt gatgacaaca tggtagtaga aaaggatatc tttttccaca
    39241 ctatgtgtga gcaccacttc ttgccatttt atggtagagc gcacatagcc tatattccag
    39301 atggtcgtgt ggcaggtttg tctaagctag cccgtacggt tgaagtttat tcgaaaaaac
    39361 cacaaattca agaacgattg aatatcgaag tggccgatgc cttgatggag tatctaggtg
    39421 ctaaaggagc ctttgttgtc attgaggcgg aacatatgtg tatgagcatg cgtggtgtca
    39481 gaaaaccagg cactgcaacc ttgacgacag tagctcgtgg tctatttgaa acagataagg
    39541 acctccgaga tcaggcttat cgtttaatgg gactataata ttcttcgaaa atcaaattca
    39601 aaccacgtca gcctccatct gcaacctcaa aacagtgttt tgagcagcct gcggctagct
    39661 tcctagtttg cactttgatt ttcattgagt ataaaaagaa tccgctttgg gcggattttt
    39721 cgagaaagga atcattatgg atcaactgca gattaaggat ttggaaattt ttgcctatca
    39781 tggtcttttt ccgagtgaga aagaattggg gcaaaagttt gtcgtttcag ctatcctatt
    39841 ctatgatatg accaaggcgg cgacagattt ggatttaaca gcctctgtcc attatggaga
    39901 actgtgtcag cagtggacga cttggtttca ggaaacaact gaggacttga ttgaaacggt
    39961 agcctacaaa ctagtagaac gtacctttga ggcttatcct cttgtccaag aaatgaagct
    40021 ggaactgaaa aaaccttggg caccggtgca tttgccacta gatacttgct cggtaaccat
    40081 tcatcgccgc aagcaacgag cctttatcgc cctaggaagc aatatgggag ataaacaagc
    40141 aaacttgaaa caagccattg agaaaatgcg aaatcaaggt atccatattc tcaaagagtc
    40201 cagtgtcttg acgacggagc cttggggtgg tgtggagcaa gatagctttg ctaatcaagt
    40261 ggttgaggtt gaaacctggc taccggcacc ggtcttgtta gaaagcttgt tagccattga
    40321 gtcagagatg ggacgggtga gagaagtaca ttgggggcct cgtttgattg atttggactt
    40381 gctctttgtg gaggaccaga tcctttatac agatgacctt atcttgcccc atccttacat
    40441 agcggaacgc ctttttgtcc ttgagtcctt acaggaaatt gcgcctcatt ttatccatcc
    40501 gacattaaag caaccgatcc gcaacttgta taatgctttg aaacaataga aaaaactcta
    40561 gttttcagta gtttgtaact gaaggctaga gtttttaaaa taggtcattt tcttctggga
    40621 ggaggatagt ttctctaccg tccatgtcta aaaccagtac tcttggggga taacgagggt
    40681 cgaaaggatg gttaaagtca aaatcaatgg ctgtagggag gtgttgactt gaaaagtgga
    40741 aggtaatctt tccttggtta ttaagcaatt gaaactcgag ttcttcttcc aattcaaaga
    40801 cattttttaa gaaatggtcg ataatatacc aaaaagagtc aatgatgtca tcaggcaagc
    40861 tggtaacaat gccaaaacta gcagaccgca tgtgggtatt ggtaaaagcc atatctctgt
    40921 ctcctttctt ttcccttatc atacagcaaa taggattaaa aatcaagaaa aggtgatttt
    40981 ttgaaaagga ttttaactac agggaaaaag aaggaaaaaa attcccaaaa atctaccgaa
    41041 gttaataggt aaattcccaa attaacttga ttatataact ttcagttact ttgagaagtt
    41101 accgaaaaat atttttcata tctattgact tttaggggtg aaatttggta tgatagtagg
    41161 cggtattgtt taccccattt gaaaggcccc ggaaccttcc aaatactttt cgatgggaag
    41221 gaacacccat caccgtaaac aaaaatcgaa ctatatatag gagaaatcat gaacaaaaca
    41281 acatttatgg ctaaaccagg ccaagttgaa cgtaaatggt acgtagttga cgcaactgat
    41341 gtaccacttg gacgtctttc tgcagtagtt gctagcgtac ttcgcggaaa aaacaaacca
    41401 acatttacac cacacactga tacaggtgac ttcgtgattg ttatcaatgc tgaaaaagtt
    41461 aaattgactg gtaaaaaagc aactgataaa atctactaca ctcactcaaa ccacccaggt
    41521 ggattgaaac aaatctctgc aggtgaactt cgttctaaaa atgcagtacg tttgattgag
    41581 aaatcagtta aaggtatgct tccacacaat acacttggac gcgctcaagg tatgaagttg
    41641 aaagtatttg ttggagctga gcacactcat gctgcacaac aaccagaagt tcttgacatt
    41701 tcaggactta tctaaggaaa ggaacaaata aagtatgtca caagcacaat atgcaggtac
    41761 tggacgtcgt aaaaacgctg ttgcacgcgt tcgccttgtt ccaggaactg gtaaaatcac
    41821 tgttaacaaa aaagatgttg aagagtacat cccacacgct gaccttcgtc ttgtcatcaa
    41881 ccaaccattc gcagttactt caactgtagg ttcatacgac gttttcgtta acgttgtagg
    41941 tggtggatac gctggtcaat caggagctat ccgtcacggt atcgctcgtg cccttcttca
    42001 agtagaccca gacttccgcg attcattgaa acgcgcagga cttcttacac gtgactcacg
    42061 taaagttgaa cgtaagaaac caggtcttaa gaaagctcgt aaagcatcac aatttagtaa
    42121 acgttaattc gaaagaatta ctatacttat acagagcacc tttcggggtg ttcttttttt
    42181 atactttctt actaaattgg tgcaattgac acagttgttg cgactttagt cgcttacaaa
    42241 tgtggctgca acctgacaag gtcagttgcc tcaaaacgtt aatcaataca attatatcaa
    42301 cgtttcaaag cactcaagag tttacctcat gggtgctttt tttatgtttt ttgaaaaagt
    42361 tcacctcaaa gtttacctta ttttcacctt attgttttag agactgtaag gcaaattcac
    42421 cgacggccat agggactaca ttagaagtat tgacataaat ctgtgttgta cctgtgtcgc
    42481 tatgtattag agcttcagaa atggcttcta agctcattcc tgcttgttta gcgagcgttg
    42541 ctccggtatg acgtagtttg tgtggcgtag cgtgtgtaag ctctgggtgt cgttttctga
    42601 tagttttcat cttattattg agatagtcag aatgtaaagg tttattgata ttgcctttgg
    42661 tatctatgta agtaaagatg aattgttctg gattactgat gataccaaac tttgccagtt
    42721 catatttttg ttgttttttc catgaagtca gaagttgaag taagtcatta gaaatagaga
    42781 aaatgggaaa tgtttccaca ataaaacgca tattatcaga tggttaatgc gttttatatt
    42841 tgggtttgag gcttataaaa taggataagc gtactgaaaa cttagatatg acaaacaaaa
    42901 taaaattttt aaaagctaca gagaaacatg gtataatgta tctaaaggag gtagcatatg
    42961 tccttagata tagataaaga aaagatgaca atcatgggaa tagcttttga aaaccgatcc
    43021 gtttttaaga gtgtttggta tgctttaagt acaaatatga ttgaaggatg gcgacctacg
    43081 gttagtgatg ttgaaaaatt acgagatgaa gctcttgctc tggggatgac ttaatgaagc
    43141 gcaactgtta tgagtcttat gactatgttg atccggataa tttatatacc gatccgggtt
    43201 cttcagttct aagaaataag caggaaggaa aaaagcacgt gagcttgaat atcgtatgat
    43261 tgctagtaag agtttgaaat tatttatcaa tcctatctta gttaatagtt taaaagtagt
    43321 ttgtttcaca actgcataag cttagaaaag ttgaaaagtg ataactcttt aataaactct
    43381 tggtttgtta aaagatattt tgtccactac attttttcaa ttgactatta tttgtcatag
    43441 atataacaga tagaacattg cattcagaat aatgaaaaac gcatcaaaaa tggtgcgttc
    43501 tgtttttatg ctaccataaa aggaggaaaa caaattgaaa atttttaagg gagagtttta
    43561 tcgaatctct gtattaacag acaagctagt aaggttagaa tactctcgaa ctggaaattt
    43621 tgaggataga acgacacaac ttatctataa tagagatttt ggccaaattt cgttagatta
    43681 tatcgagaca tcaaacgtac tagatattat gacggactat tttcatctgc actttaataa
    43741 aggagaattt aacgccgaaa atttatttat agaattaaaa ggaaattttg ccgtatatgg
    43801 tagtcgctgg tattttggtg aatctattga aacgttaaaa ggaacagctc ggactctgga
    43861 taaggcagat ggagcaatct cgttagaaga tggaattatt agccgaaatg gtatagcctt
    43921 attggatgat tctcaaggat ttatttggga tgaacaatct ggttatattg agagagaaaa
    43981 tcaaattgac ctgtatttct ttgcctatgg gcatgattat agaggagcaa tcagagactt
    44041 ttaccatttg actggttcaa cacccttgtt gccaagatat gctttaggca attggtggag
    44101 tagatattgg ccttatacgt cggatgaata cttggattta atagacagat ttgaaacaga
    44161 gaaaattcca ttatctatcg gtgtgttaga tatggattgg catataactg acattccagc
    44221 tcgttttgga agtggctgga caggatatag ttggaataga aacttaatac caaatccaga
    44281 acagttattg caacaacttc atgatagaaa gttaaaactc tccttaaatg tccatcctgc
    44341 tgatgggata cgggcttatg aagaagctta tcctcaaatc gcaaaacggt tggggttaaa
    44401 tgtagaacta gaagaacctg ctatttttga tttttttaat ccctctttta gggaagccta
    44461 ctttaaagat gttcattatg agctagaaaa gcagggagta gatttttggt ggattgactg
    44521 gcaacaaggg acacaaggta tgctagatcc actttggctt ttaaaccatt atcactatca
    44581 ggatagttgt aaaaatgcag aaggtggttt gattttatca agatatgcag gtcctggtag
    44641 tcaccgctac cctgttggtt tttcagggga tactattatt agttggaatt ccttaaggtt
    44701 tcaaccctat tttacagcga cagcatctaa tatcggttat agttggtgga gtcatgatat
    44761 cggtggacat atgttggggg attatgacga agagctacaa actagatggc tacagtttgg
    44821 tgtttttagt ccgattactc gattacatag ttctagaagt ccttttaata gtaaagaacc
    44881 ttggtttttt tcagaaacaa catctaagat tatgaagaaa taccttcgtt tgagacatca
    44941 gatgattcct tatctatata ccatgaatgt aaagacacat gaggaaggtg ccccattaat
    45001 cagtccaatc tattatttct atccagagaa tgatgagagc tataatgttc caaaccaata
    45061 cttttttgga acagaactga tggtggctcc cattgtagaa aagatggatt tgacattcca
    45121 atctgcaaaa gtagatgtat ggttccctga aggtgaatgg tatgacttct tttcagagaa
    45181 aaaatacaca ggtggtgtga agttaagtgt ttatagggac atctcgacta cgcctgtgtt
    45241 tgcaaaaagt ggtgcaatca ttcccttggt tggttctgag ataggtatgg gtgttgattt
    45301 acctgaggtt gtagattggt atgtattccc agggaaacaa cattcttttg aaatgcttga
    45361 agatcaaaat ggtcaaagat ataaaacaag attatcaatc gattgggaaa tgggaatggt
    45421 agagttagca ttacaaggag attctagtat cgttccaagc aatagaaaac atagaattca
    45481 ttttaaagga acgaatgtgt ctataattga attgccaaat aagaatgata cagctaaatt
    45541 tgaatggaaa gataataaaa ggacatcttt aaatgacgaa gtttttagac tactaaagac
    45601 agcttctctt ccatatgaat taaaagatag attgttaaat caattcatca atgcgaaaaa
    45661 ctctcatgac ttaatgaata tcttgcatca tcaggataag gaattgagag ggcgtttgtt
    45721 ggaaatgata tttactagcc aaaactaatt gaaataatta gaaagtcatt tgtttagtgt
    45781 atatgaagat tctgtatggt attcgttatc actggtcaca ttgaaaatgt gaaataagca
    45841 cctacaacat agactttaca acacttcatg tttcatatca tggggtgttt ttctatttaa
    45901 gtcaaagcca atcaaaaaat ctcactattc atcaggttaa aggaaaccta atcatagtga
    45961 gattgtgaat tatagtagag tttgtatcgc ctcttcaatt tgttttgggt ctgtttttga
    46021 agaaaagcgt tcaaagactt gcccatcacg accaatgaga aacttagcga aattccattc
    46081 gattcgtttt cctagtgggc cagatttatg gtcttttaac cagacataga gagggtctgc
    46141 ttccttaccg ttgaccttaa tcttggcaaa acgtgggaag gtggtttgaa aatgtaggct
    46201 acagaaggtg ttgatttcct ctgcgctgcc gggtgcttgt cccataaact gattgcaagg
    46261 gaaatccaat atttcgaagc cctgatcttg atagcgttca tagagttctt gaagtctctg
    46321 gtactggggc gttaaaccac atccagtagc agtgttgaca atcaagagaa ctttgccacg
    46381 atagctatcc aagggagttg cttggttgtt ttggttcaaa acggaaaaat cataaagtga
    46441 agtcatgagg gcctcttttc tttttctaca ttgtaacagt ttttactttt ttcgtcgagt
    46501 cggatggaag atattgttga agaataagcc ctcaatatga tagaaaccgt ttacaaataa
    46561 ctttaaaaat ggtatgattt aattaaaccg gtttactttg ttgctataaa tctctaattt
    46621 gagatttagt tcctattgat tttacaatat gtttattgga gtgtatacat gcaaacaaaa
    46681 acaaagaagc tcattgtgag tttgtcttca cttgttttat caggattttt attaaaccat
    46741 tatatgacag ttggagcgga agaaacgact acgaatacca ttcagcaaag ccagaaggaa
    46801 gttcagtatc agcaaaggga tacaaaaaat ttagttgaaa atggtgattt tggtcagacg
    46861 gaggacggaa gcagtccgtg gacaggaagc aaagctcagg ggtggtcagc ttgggtagac
    46921 cagaagaata gttctgcaga tgcctcaact cgagtcattg aggctaagga tggggctatc
    46981 actatctcaa gccctgagaa attaagggca gcggttcacc gtatggttcc tattgaagct
    47041 aagaaaaagt ataaactgcg tttcaagatt aaaacagata ataaagtcgg gattgccaag
    47101 gttcgtatca ttgaggaaag tggtaaggac aagcgattgt ggaattctgc aacgacgtca
    47161 ggaacaaagg actggcagac cattgaagca gactatagcc cgactttaga tgttgataaa
    47221 atcaagctgg agttattcta tgaaacagga actgggactg tttcctttaa ggatattgag
    47281 ctggtagagg tagcagccca gctttctgag gattctcaaa cagataaaca gcttgaggaa
    47341 aagattgatt taccaattgg aaaaaaacat gttttttctc ttgcggacta tacttataag
    47401 gtagaaaatc ctgacgttgc ttcagtcaaa aatggaattt tagaacctct taaggaaggg
    47461 acaaccaatg tcattgtcag taaagatggc aaggaagtga aaaagattcc tttgaagatt
    47521 ctagcctctg ttaaggatgc atacacagcc cgtttggatg actggaatgg catcatcgct
    47581 gggaatcaat actatgattc taaaaatgaa cagatggcca aattaaacca ggaattggaa
    47641 ggaaaggtag ctgatagcct atctagtatt tcaagtcagg cggaccgcac ctatttgtgg
    47701 gaaaaatttt caaattataa aatgtctgca aatctgactg ccacttatcg gaaattggag
    47761 gagatggcca agcaagtgac caatccttct tctcgttatt atcaagatga aactgtcgtt
    47821 cgaacagtca gggattccat ggaatggatg cataaacatg tctacaatcg tgaaaagagc
    47881 attgttggga actggtggga ttatgaaatc ggtacacctc gtgccatcaa caataccttg
    47941 tctctgatga aagaatactt ctctgatgag gaaattaaaa aatatacaga tgtgattgaa
    48001 aaatttgtac cagatcccga acatttccga aagacgactg ataacccatt taaggctcta
    48061 ggtggaaact tagttgatat gggaagagta aaagtaatag ctggtttact gcgtaaagat
    48121 gatcaagaaa tttcttctac cattcgctcg attgagcaag tgttcaagtt ggtagaccaa
    48181 ggtgaaggtt tttatcaaga tggatcctat atcgaccaca ccaatgttgc ctatacgggt
    48241 gcttatggga atgttttgat tgatggcctg tctcaactgt tgccagtcat tcaaaagacc
    48301 aagagtccaa tcgataaaga taaaatgcaa accatgtacc actggattga taaatcgttt
    48361 gctcctttgc tggtgaatgg agaactgatg gatatgagtc gtggacgctc gatcagtcgt
    48421 gcaaatagcg aggggcacgt ggccgcagta gaagtactaa gagggattca ccgaatagcg
    48481 gatatgtctg aaggagaaac caaacaacgt ttgcagagtc ttgtgaagac cattgttcaa
    48541 tcggatagtt attatgatgt ctttaagaat ttgaagactt ataaggatat cagtttgatg
    48601 caatccttgt taagtgatgc aggagtcgca agtgttccaa gaccaagtta cctatctgcc
    48661 tttaacaaga tggataaaac agccatgtac aatgcagaga aagggtttgg atttggcttg
    48721 tcactctttt ccagtcgtac cttgaattac gaacacatga acaaggaaaa taaacgtggt
    48781 tggtatacga gtgatgggat gttctatctt tacaatggcg atttgagtca ctatagcgat
    48841 ggctactggc caacagttaa tccatataag atgcctggta caacagagac ggatgctaag
    48901 agagcggata gcgatacagg taaagtttta ccgtctgctt tcgttggaac gagcaaacta
    48961 gatgatgcca atgcgacagc aaccatggat ttcaccaact ggaatcaaac attgactgct
    49021 cataagagct ggtttatgct aaaggataag attgcctttt taggaagcaa tatccaaaac
    49081 acttcaacag atactgctgc aactacaatc gaccagagaa aactggaatc aagtaatcca
    49141 tataaagtct atgtcaatga taaagaagcc tcccttacag aacaagaaaa ggattatcct
    49201 gaaacccaaa gtgtcttttt agaatcgtcc gattcgaaaa agaatattgg ttactttttc
    49261 tttaagaaga gttcaatcag catgagtaag gctttgcaaa agggagcctg gaagggtatc
    49321 aatgaaggac agtcagacaa ggaagttgaa aatgaatttc ttacgattag tcaggctcat
    49381 aagcaaaatg gagattctta tggctatatg ctcattccta acgtggatcg tgccaccttc
    49441 aatcaaatga taaaagagtt agaaagtagc ctcatcgaaa ataacgaaac ccttcagtct
    49501 gtttatgatg ctaaacaagg agtttggggc attgtgaaat atgatgattc tgtctctact
    49561 atttccaacc aattccaagt tttgaaacgt ggagtctata ccattcgaaa agaaggggat
    49621 gaatataaga ttgcctacta taatcctgaa acccaggaat cagctccaga tcaggaagtc
    49681 tttaaaaagc tagagcaagc agctcagcca caagtacaga attcaaaaga aaaggaaaaa
    49741 tctgaagagg aaaagaacca ttcggatcaa aagaatctcc ctcagacagg agaaggtcag
    49801 tcaatcttgg caagtctagg gttcttgcta cttggggcat tttatctatt ccgtagagga
    49861 aagaacaact aatttgttca tcatctagat gaattacatg aaactgtgga aaatagaaaa
    49921 aatccgaaga aggccaaata tcttcggatt tttcttttaa aaaaggataa gttgatactt
    49981 atcctttttt acttcattat tatgtgacta tagcgttttg aaactagaat agaacgt
//
LOCUS       DAVMGZ010000003        46259 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000003 DAVMGZ010000000
VERSION     DAVMGZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46259
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.003"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            240..446
                     /locus_tag="VYQ93_000103"
     CDS             240..446
                     /locus_tag="VYQ93_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3334866.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
     gene            443..637
                     /locus_tag="VYQ93_000104"
     CDS             443..637
                     /locus_tag="VYQ93_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3334867.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            624..1508
                     /locus_tag="VYQ93_000105"
     CDS             624..1508
                     /locus_tag="VYQ93_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3334868.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIDPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENIIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            1510..2247
                     /locus_tag="VYQ93_000106"
                     /pseudo
     CDS             1510..2247
                     /locus_tag="VYQ93_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2247..3344
                     /locus_tag="VYQ93_000107"
     CDS             2247..3344
                     /locus_tag="VYQ93_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3334869.1"
                     /translation="MLERLKSIHYMFWISLIFMVFPILTVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGILLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     AKQNAQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            3346..3945
                     /locus_tag="VYQ93_000108"
     CDS             3346..3945
                     /locus_tag="VYQ93_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3334870.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEMVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            4370..5380
                     /gene="ccpA"
                     /locus_tag="VYQ93_000109"
     CDS             4370..5380
                     /gene="ccpA"
                     /locus_tag="VYQ93_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV3334871.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(5586..6548)
                     /locus_tag="VYQ93_000110"
     CDS             complement(5586..6548)
                     /locus_tag="VYQ93_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV3334872.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            6614..8002
                     /locus_tag="VYQ93_000111"
     CDS             6614..8002
                     /locus_tag="VYQ93_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3334873.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            complement(8037..8489)
                     /locus_tag="VYQ93_000112"
     CDS             complement(8037..8489)
                     /locus_tag="VYQ93_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV3334874.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            8652..8945
                     /locus_tag="VYQ93_000113"
     CDS             8652..8945
                     /locus_tag="VYQ93_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3334875.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVVKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            9101..10315
                     /locus_tag="VYQ93_000114"
     CDS             9101..10315
                     /locus_tag="VYQ93_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV3334876.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            10460..10594
                     /gene="rpmH"
                     /locus_tag="VYQ93_000115"
     CDS             10460..10594
                     /gene="rpmH"
                     /locus_tag="VYQ93_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV3334877.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            10857..11522
                     /locus_tag="VYQ93_000116"
     CDS             10857..11522
                     /locus_tag="VYQ93_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV3334878.1"
                     /translation="MTILGKDTVQQSAKGESVTQEATPEYKLETTPGGDKGGNTGSSD
                     ANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKNAIEKAAKNKQDEIKGAPL
                     SDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIAMVTVPKRLVAPNAA
                     PKTTSAPQATAGTMQNVTYQSPAGKQLPNTGSASSAALASLGLVVATSGFALLGRKTR
                     RRK"
     gene            11827..12600
                     /locus_tag="VYQ93_000117"
     CDS             11827..12600
                     /locus_tag="VYQ93_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV3334879.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMIISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            12600..13160
                     /gene="rnmV"
                     /locus_tag="VYQ93_000118"
     CDS             12600..13160
                     /gene="rnmV"
                     /locus_tag="VYQ93_000118"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV3334880.1"
                     /translation="MKEKISQVIVVEGRYDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            13232..14131
                     /locus_tag="VYQ93_000119"
     CDS             13232..14131
                     /locus_tag="VYQ93_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3334881.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            14451..14747
                     /locus_tag="VYQ93_000120"
     CDS             14451..14747
                     /locus_tag="VYQ93_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV3334882.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            14824..16854
                     /locus_tag="VYQ93_000121"
     CDS             14824..16854
                     /locus_tag="VYQ93_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV3334883.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQGLFTDYL
                     QNFSSESVEVTSQKYYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDCLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            16856..17497
                     /locus_tag="VYQ93_000122"
     CDS             16856..17497
                     /locus_tag="VYQ93_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3334884.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            17490..17783
                     /locus_tag="VYQ93_000123"
     CDS             17490..17783
                     /locus_tag="VYQ93_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3334885.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            18034..18906
                     /gene="rsmA"
                     /locus_tag="VYQ93_000124"
     CDS             18034..18906
                     /gene="rsmA"
                     /locus_tag="VYQ93_000124"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV3334886.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLMPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            18908..19786
                     /gene="rsgA"
                     /locus_tag="VYQ93_000125"
     CDS             18908..19786
                     /gene="rsgA"
                     /locus_tag="VYQ93_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV3334887.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            19797..20453
                     /locus_tag="VYQ93_000126"
     CDS             19797..20453
                     /locus_tag="VYQ93_000126"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV3334888.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            20416..21078
                     /locus_tag="VYQ93_000127"
     CDS             20416..21078
                     /locus_tag="VYQ93_000127"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV3334889.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            21080..22336
                     /locus_tag="VYQ93_000128"
     CDS             21080..22336
                     /locus_tag="VYQ93_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV3334890.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLYFQENEEEYED"
     gene            22326..23267
                     /locus_tag="VYQ93_000129"
     CDS             22326..23267
                     /locus_tag="VYQ93_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV3334891.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            23534..24361
                     /gene="purR"
                     /locus_tag="VYQ93_000130"
     CDS             23534..24361
                     /gene="purR"
                     /locus_tag="VYQ93_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV3334892.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            24428..25678
                     /locus_tag="VYQ93_000131"
     CDS             24428..25678
                     /locus_tag="VYQ93_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV3334893.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            25804..26598
                     /gene="pflA"
                     /locus_tag="VYQ93_000132"
     CDS             25804..26598
                     /gene="pflA"
                     /locus_tag="VYQ93_000132"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV3334894.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(26673..27599)
                     /gene="yidC"
                     /locus_tag="VYQ93_000133"
     CDS             complement(26673..27599)
                     /gene="yidC"
                     /locus_tag="VYQ93_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV3334895.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(27677..27955)
                     /locus_tag="VYQ93_000134"
     CDS             complement(27677..27955)
                     /locus_tag="VYQ93_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV3334896.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            27989..28729
                     /locus_tag="VYQ93_000135"
     CDS             27989..28729
                     /locus_tag="VYQ93_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV3334897.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            28785..29468
                     /locus_tag="VYQ93_000136"
     CDS             28785..29468
                     /locus_tag="VYQ93_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV3334898.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            29580..30572
                     /locus_tag="VYQ93_000137"
     CDS             29580..30572
                     /locus_tag="VYQ93_000137"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV3334899.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            30637..31176
                     /gene="rsmD"
                     /locus_tag="VYQ93_000138"
     CDS             30637..31176
                     /gene="rsmD"
                     /locus_tag="VYQ93_000138"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV3334900.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            31166..31654
                     /gene="coaD"
                     /locus_tag="VYQ93_000139"
     CDS             31166..31654
                     /gene="coaD"
                     /locus_tag="VYQ93_000139"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV3334901.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            31638..32675
                     /locus_tag="VYQ93_000140"
     CDS             31638..32675
                     /locus_tag="VYQ93_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV3334902.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHLDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            32733..32963
                     /locus_tag="VYQ93_000141"
     CDS             32733..32963
                     /locus_tag="VYQ93_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV3334903.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            32987..34270
                     /gene="murA"
                     /locus_tag="VYQ93_000142"
     CDS             32987..34270
                     /gene="murA"
                     /locus_tag="VYQ93_000142"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV3334904.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            34263..34451
                     /locus_tag="VYQ93_000143"
     CDS             34263..34451
                     /locus_tag="VYQ93_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV3334905.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            34490..35314
                     /gene="endA"
                     /locus_tag="VYQ93_000144"
     CDS             34490..35314
                     /gene="endA"
                     /locus_tag="VYQ93_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV3334906.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            35605..36936
                     /locus_tag="VYQ93_000145"
     CDS             35605..36936
                     /locus_tag="VYQ93_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV3334907.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELISQNKQFILTNDKVKNGRVTKVKIQITEVEIEEETE"
     gene            complement(37134..37204)
                     /locus_tag="VYQ93_000146"
     tRNA            complement(37134..37204)
                     /locus_tag="VYQ93_000146"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37170..37172),aa:Cys,seq:gca)
     gene            37620..41231
                     /gene="rpoB"
                     /locus_tag="VYQ93_000147"
     CDS             37620..41231
                     /gene="rpoB"
                     /locus_tag="VYQ93_000147"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV3334908.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            41264..44941
                     /gene="rpoC"
                     /locus_tag="VYQ93_000148"
     CDS             41264..44941
                     /gene="rpoC"
                     /locus_tag="VYQ93_000148"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV3334909.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            45052..45465
                     /gene="ndk"
                     /locus_tag="VYQ93_000149"
     CDS             45052..45465
                     /gene="ndk"
                     /locus_tag="VYQ93_000149"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV3334910.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
BASE COUNT        13497 a         8752 c        10733 g        13277 t
ORIGIN      
        1 gtgtttgtct gataaaaatt tctactcttt ttgattttaa ataagtatta gtttacatta
       61 tggtgtgaat tgggtttgat atctcttttg aggaagttgc cttagatttt tctgattgtg
      121 ttttattgta tagtgtatct tgcttgtttt gaacagaatt tttatgacat ttgtcatatt
      181 ttctagtgac agaagcttct gcctcctctg attttaaaag actataattg tagtatgaaa
      241 tgggggaaga agagatgaga aataaaatga ttattgcaat gagtttagta gtagcaggag
      301 ttatgaccta tctcatgttt tcgggattgg atgaggattt ctaccatttt ccttggaagg
      361 tctttgctgg ctttggaatc atgtcttggc ttgttagaga aggtttgaaa ttagtcagag
      421 atgtgaaaaa ggagtttgag gaatgaagag agggatcatc tatttcttta tcggcctgtc
      481 actcttggta tggttagtgg aaatgtttac tgattggttt gatcaagcct tgcttcgcca
      541 attcatttgt ggtgctttgg ggtttggatt tatgattttc gtcgttttcc ctatgggaat
      601 ggagtggttg aaaggagagt ctcatgactg tggttaaagt tgaaaaattg agtaagaaaa
      661 taaaagacaa ggaaattttg cggaacatct cctttgaaat caacgatggt gaatgtgttg
      721 ccttaattga tccaaatggt gccggaaaga caacactctt agattgtttg cttggagata
      781 aactggtcac aagcggtcaa gtatctattc aaggtttacc agtgacgagt tctaagttag
      841 actatactag ggcttacctc cctcaagaaa atataatcgt tcagaaatta aaagttaaag
      901 agttgattgc tttctttcaa cgtatctatc caaatccttt gagcaatcag gaaattgatc
      961 aactattgca gtttgtcaag caacaaaaag aacagttggc agaaaaatta tcaggcggtc
     1021 aaaagcgtct cttttctttc atcttgacct taattgggcg accaaagatt gtttttttag
     1081 atgagcctac tgcgtccatg gatacctcaa ctcgtcaacg tttttgggaa attgtccagg
     1141 agttaaaagc gcaaggagtc accattctct attcgtccca ttatattgag gaagtggaac
     1201 acactgcaga ccgcattttg ctcttaaata agggagagtt gattcgagat acgacgcctc
     1261 tagctatgcg tagcgaggaa atagaaaagc actttatcct tcctatagct tacaaggaag
     1321 tcgtagagca gtcaaatttg gttgaaaact ggaccctaaa gcaagattct ttacaagtag
     1381 tcactcgaga agcagatgct ttctgggaac tattagccca agcaggatgt aggatgcaag
     1441 aaatcgaagt taataatcgt agtttgttga atacaatctt tgaagagacg cagaagggag
     1501 ataactaaga tgaaacgatg gatagcactt agtaagatag aatttttatt gaccaaacga
     1561 caattaatct attatctatt gtcggtaggg atgccgacgg ctttctattt atttttttct
     1621 ggtatctatc aggacacacc aggtgaactc gcgaatttta tgcgtgatta tcttatctcc
     1681 atgactgcat tttccatgat gtcaacagct atcttctcat tcccagttgt tttacatacc
     1741 gacaagatca acaactggca gaaaacattg cgtcatagcc ctgtaaatat ggtagaatat
     1801 tatctatcaa agataacaag tatgctggtt gattatttgg tttcaatctt ggtggttttc
     1861 tcagttgggc attttgtaag aggtgtggac atgtctcttg gaaattggat tggggctgca
     1921 cttttgctga tagtaggaag tattgccttc gtagcgcttg gtttgacctt gactctctta
     1981 ccaactagtt agttgatgtc tgtcgtgggc aatcttcttt atctagggtt ggctgtttta
     2041 ggtggactct ggatgcctat ctctttattt ccagactgga tgcaagtagt aggtaagtgt
     2101 ctcccgacct atcagttgat ggagttgctc aagaccttct taaatgaggg tggcatcaat
     2161 ctatcagcca cagtttattt acttgttttt tcagtagttt tgtttggttt gaccatttac
     2221 cttcaaggtc ataaggagaa tgcttaatgc ttgaaagact gaaaagcata cattatatgt
     2281 tttggatcag tttaattttt atggttttcc ccatcctaac tgtagtgact gggtggcttt
     2341 ctgcctggca tttattgatt gatattctat ttgtagtggc gtatttgggt gttttaacaa
     2401 ctaagagcca gcgtctatct tggctatatt ggggcatcct gctgacttat gtagttggga
     2461 atactgcctt tgtggctgtt aattatatct ggtttttctt tttcctatcc aatctcttaa
     2521 gctatcattt cagcgtaggt ggtttaaagt ctttacatgt ctggactttt cttcttgctc
     2581 aagtccttgt tgtgggtcaa ctgttgattt ttcagagaat cgaagttgag tttctattct
     2641 atctacttgt aattcttgct tttgtcgatt taatgacttt tggcttggtt cggattcgaa
     2701 ttgtggagga tttgaaagaa gcacaggcta agcaaaatgc ccagataaat ctattgcttg
     2761 ctgaaaatga acgcaatcgt atcggtcaag atttgcatga tagtctgggg catacctttg
     2821 ctatgcttag tgttaaaact gatctagcct tgcagttatt tcagatggag gcttatccac
     2881 aggtggaaaa ggaattaaaa gaaattcacc agatcagcaa ggattccatg aatgaagttc
     2941 gaactatcgt ggaaaatctt aagtctagaa ctttgacatc cgaactagag actgtgaaaa
     3001 agatgttaga aattgctgga attgaggtgg aaacggataa ccaactagat actgctagcc
     3061 ttactcaaga attggagtca atggcttcta tgattttgct tgagttggtg accaatatca
     3121 tcaaacatgc caaagcgtct aaagcttact taaaattaga acggacagag aaggaactca
     3181 ttttaacagt aagtgatgat ggctgcggct ttgcttttct aaaaggagat gagctccata
     3241 cagtccgaga tcgtgttttt ccattttcag gagaagtaag tgtaatcagt cagaaacatc
     3301 caacggaagt gcaagttcga ctaccttata aggagagaaa ctaagatgaa agtattagtc
     3361 gcagaagatc aaagtatgtt gcgagatgcc atgtgtcaat tgctcacgct tcaaccagat
     3421 gtggagtctg tccttcaagc caagaatggg caagaagcaa tccaactatt agaaaaggag
     3481 tctgtagata tcgccatcct tgacgtagaa atgcctgtta agacaggtct tgaagtcttg
     3541 gagtggatac gatcagaaaa gcttgaaaca aaggtggttg tggtgacgac cttcaagcgt
     3601 gctgggtatt ttgaacgtgc ggtcaaggct ggagtggatg cttatgtatt aaaggaacga
     3661 agcattgcag acctcatgca aaccttgcac accgtcctcg aaggacgcaa ggagtattcg
     3721 cctgaattga tggaaatggt gatgacccgt cccaatccgt tgacagaaca agagattgct
     3781 gtcttaaagg gaatcgcccg gggcttatcc aaccaagaaa tcgcagatca gctttacctc
     3841 tcaaacggaa ctattcgaaa ctatgtcacc aatattcttt caaaactgga tgctggtaat
     3901 cgaacagagg cagctaatat cgcaaaagaa tctggttggt tatgatgata taatattttc
     3961 atgaagacta tgtgctaaaa ttgacggtag tgaatggaac aatacctata gtagattgaa
     4021 actagaatag tacacctcta cttctaaaac attgttagaa atcgatttga ctgtcctgat
     4081 cgatttatcc tgttcttatt tcattttact ataactttag gagggcttag tttcctcctt
     4141 tttgtttagt ctaaggtggt agcaaggagc taaacctagg ttttctatga aattttttca
     4201 atcaatcttc aaaaaacact tgaaagtgtt tacaattagt gatagaatgg aataagtaga
     4261 aatatgaaaa cgaaattttc acatcttcac ttttgtaagt agatatagag cttcaatcag
     4321 tacctctact tgtaaaagaa tgtgtgagat agaaaggaca ggaaaaggaa tgaatgcaga
     4381 tgatacagta accatttatg atgtcgctcg tgaagcaggt gtttccatgg cgacggtcag
     4441 ccgtgtggtc aatggcaata aaaatgtaaa agagaatacc cgtaaaaaag tgctagaggt
     4501 aattgatcgt ttggattatc gtccaaatgc agttgcgcgt ggtcttgcaa gtaaaaagac
     4561 aaccactgtc ggtgtcgtga ttccaaatat taccaatggt tatttttcga gtttggctaa
     4621 ggggattgat gatattgcag aaatgtacaa gtacaatatt gtcctagcta atagcgatga
     4681 agataacgag aaagaagttt ctgttgtcaa taccctcttt tcaaagcagg tagatggcat
     4741 tatctatatg gggtatcact tgacagataa aattcgctca gaattttcgc gttcacgtac
     4801 tccgattgtt ctcgcaggaa ctgtcgatgt tgagcaccag ttgccaagtg tcaatattga
     4861 ctataagcaa gcaacaattg atgcagtgag ttaccttgct aaagaaaatg agcgtattgc
     4921 tttcgttagc ggtccgctag tggatgacat caatggtaag gttcgtttag ttggctacaa
     4981 ggaaaccttg aaaaaagcag gaatcactta tagtgagggt ttggtatttg aatctaaata
     5041 tagctatgat gatggttacg ccttagcaga gcgtttgatt tcatcaaatg caactgcagc
     5101 agttgtgaca ggtgatgagt tggcagcagg agtcttgaac ggtttggctg ataagggtgt
     5161 ttctgtgcca gaagattttg aaattattac tagtgatgat tcacaaatct cacgctttac
     5221 ccgtccaaac ttgacaacga ttgcccaacc tctttatgac cttggtgcca ttagtatgcg
     5281 tatgttgacc aagattatgc ataaggaaga gttggaagaa cgtgaagttc tcttacctca
     5341 tggtttgaca gaacgtagct caacacgaaa acgtaaatag aaaaaatcag ggaatcgaga
     5401 agatttcctg attttgtgct cttaaacaag agatgttgac ttaatgaata ccatagagcg
     5461 gactaggcgg ctggttaaag gctgcgcaac acactgtttt gaggttgtgg atgaaactga
     5521 cgaagtcagt aaccatacat acggtaaggc gacgtgacgt ggtttgcaga gattttcgaa
     5581 gagtattagc cttccatata gtctttcaaa gcctgtcctg ttagtccggc attgagggcg
     5641 atgaggagtt tcaagcgagc tttttgggca ttgagttctt taacaaagaa aacgcctgct
     5701 ttttgcaact gtacgccccc accctggtag gcataaacag gctcggcaat accgttaaag
     5761 catcgtgata ccagagcgac tgggattcct ttttgcagaa ggctttctaa tttttgagcc
     5821 gtttctttgg gaatattacc agctccgaag gcttggataa tcaaaccgtc caagtgttct
     5881 aaatccagca tatcaatcag ctcatctgtc ataccagcat aagccgagat gatagggact
     5941 aaaccttgta tgtgatcaag gtcaaagcga acacgaggtt cagctgtttt gaagtagagg
     6001 atttcctgtt tcatgatgag accgaggggg ccatgtgttg gagtctggaa ggtgctgaca
     6061 ttagtcgtat gtgttttggt gacatacttg gcagcgtgga tttcatcgtt cataacgacc
     6121 aaaactcctt tgtcagcagc cctgtcatcg ctggccactc gtaaagcact taggtaatta
     6181 taaacaccat cactaccgag ctcattggag ctacgcatgg ctcctgttag aacgataggc
     6241 atatggggaa cttccatggt atcaaggaaa taggctgttt cctctaaagt atcggttccg
     6301 tgtgtgatca ccactccatc gtagttatct gcttcctctt taattttctg gtagaggacc
     6361 agcatatgtt tgggtttgat atggggactt ggaaggttaa aaaagtccaa ggcgtggact
     6421 tggattcctt caagtgggtt ggacacatgg ttcatgggat tatctgaact cgtcacaaca
     6481 gcgccagaag catcggcctg catggaaata gttccacccg tatgtaaaac aaggattttc
     6541 ttaggcatga tttactcact ctttctgaaa tgtggtataa tcattgtatc acaaaagggg
     6601 agattgggat aggatggaag tcaaagctgt tttttttgat atcgatggaa ccttggtcaa
     6661 caatcgcaag agtgttttga aatccactaa ggatgcgatt aagattgtca aagaacaagg
     6721 ggtactagtt ggcgtagcga cagggcgagg accttttttt gttaaggaat tgatggacga
     6781 tttggatctg gattttgcgg taacctataa tggccagtat atctttagta aagacagagt
     6841 cttgttcacg agccctattt ccaagttaca tttgcgccat ctaattagct atgctaaaaa
     6901 agagggcacg gagattgctc tagggaccaa ggatgccatg ttgggttcta aaatcatgtc
     6961 ctttggtctg ggttcttttt cccaacgaat tagtcgcttc gttccctctg ttttaactcg
     7021 gacggtgagt cagtctttta atcgcatggt cagcaaggtt gttccccaaa aggaagagga
     7081 cctgcttcac ctgatgaatc agcctatcta ccaagttttg atgctgatga caccagaaga
     7141 atctgagaag gcggcagctg attttgaaga cttgaaattg acacgtagca atccttttgc
     7201 atcggatgtc atcaatcagg gaaattctaa attagaaggc attcgccgag ttgggaaaga
     7261 atatggcttt gacctcaacc aagtcatggc ttttggtgac tcagataatg accttgaaat
     7321 gctggctggc gtcggtatgt cggtcgctat gggaaatggt agtagcagtg tcaaggaagc
     7381 tgccaagcac attaccacta gcaatcaaca agatgggatc cacaaggcct tggaacattt
     7441 tggtgttttg tcttcagaaa aagtttttgt cagccgtgac tatcatttca ataaggtcaa
     7501 gaccttccac cacatgatgg atgaacgaac ccaagaagaa cctcgagctt gggatttaga
     7561 gggtgcaacc catagggctg gttttaaaat agaagaattg gtggagtttg ttcgatcagc
     7621 cagtccttct gaagaagatt ttggtcaagc tgtatcgcaa cttcatcagg cccttgataa
     7681 ggcagcaggt aaagtagcca agaagacacc tgctcagcaa gatttgatag ggcaagtgga
     7741 tgccttgatt gacacgcttt acttcaccta cggcagtttt gtcttgatgg gggtggaccc
     7801 agaacgtatt tttgatattg tccatcaggc caatatgggg aaaatttttc cagatggcaa
     7861 ggctcatttt gatccagtga cccataaaat cttaaaacca gataattggg aagaaaaata
     7921 tgctccagaa cctgctatta aaaaggaact gcagcgccag ctcaaggctt acgaacgcca
     7981 taaagagaga aataaatcgt aaacaaaaag gagctagggg ctccttttct ttgtgattat
     8041 aaggtttttt cttgttctct cacaaccagc aaatcgacct tagcatggcg gagtatgtat
     8101 tcagatgaag agccgactaa gaggcgttca aaggcgttga gaccagttgc gccaacgagg
     8161 atgaggtcca cttcctcggc atctggaata gtacgtgcta gcagggtctt tggatttccc
     8221 atttcaatga cgatatgaac atctgctacc ccagcatctt tagcacgttt ttcgtactct
     8281 ttcatcagac tttcagcgtc gacttggagt tcttcgtaaa cttcagcatc aaaggtggat
     8341 acgctttgga gagcgcgtgt gtcaatgaca tgtgcgatgg tgagtttagc gtcgtttcgt
     8401 agagcagaat gaactccctt gacaaaagcc aagtccgctt ccttagaacc atcgattgcg
     8461 accatgatat tttggtaacg ttgtgccata atgaaacctc ctgaaatttt cttactataa
     8521 ttgtaaacct tttcactata gaagtaaagc aaaaaaaagt atttttcaaa agaatattag
     8581 ggtttaaact ctatataaat atatgataaa ataaaagaaa ggatagcagg aaagaagtga
     8641 aggaagggag aatgatgaaa aactcttttc aaaaatcaaa tttcctttat tatggaatta
     8701 tcctagtttc agtcttggta gaagtgatca tgatctgtca attagagagt ctggtaccgc
     8761 ttctttatcc aagttttatt ggatttttag tctttcatgt actctaccat ctaatcttat
     8821 ttctagttgt aaaacgaagt gggcgttggg attacttgat gatatggggg cttttcctca
     8881 tgtttaatct tctttatgac tcctttctag gcttgctttt tctaggttta tcttttggta
     8941 tgtgatgtgt ctttgcaaaa tacctgctat ctctacgttc ttaacctcgc ctttagcaag
     9001 gtttttcttc tatacgaaac tagtaacaca tttcagtaaa cttgtcgcat tctctttcta
     9061 gtggtataat gttactatcc tgatgaattg aggaaacaag atgaaagaat ataacaagtc
     9121 tagtaagtta gagcatgttg cttatgatat ccgtggtcct gtattggaag aagccatgcg
     9181 gatgcgagcg aatggagaaa agattttacg tctgaataca ggaaatccag cagaatttgg
     9241 ctttacagcg ccagacgagg tcattcatga cttgattatg aatgcgcgtg atagtgaagg
     9301 ctattctgac tccaaaggga ttttctcagc ccgtaaggcc atcatgcagt attgccagct
     9361 gaagaaattc ccaaacgtag atattgatga tatttacctt ggaaatggtg tcagtgagtt
     9421 gattgtcatg tccatgcagg ggcttttgga caatggggat gaggtcttag tgccaatgcc
     9481 agactatcct ctatggacgg cggctgtcag cctagctgga ggaaatgccg ttcactatat
     9541 ctgtgatgaa gctgttgaat ggtacccaga tattgacgat atcaagtcaa aaatcacttc
     9601 caataccaag gcaatcgtcc ttatcaatcc aaataaccca actggagccc tttatcctaa
     9661 ggaactcttg ctggagatta ttgagattgc ccgtcaaaac gatttgatta tctttgcgga
     9721 tgaaatctat gaccgcatgg taatggacgg acatgtgcat acgcctgtgg cgagcttggc
     9781 accagatgtc ttctgtgtca gcatgaatgg tctgtcaaaa tcccaccgca tagcaggttt
     9841 tcgtgtgggt tggatggtct tgtctggccc taagactcat gttaagggct atatcgaagg
     9901 gctcaatatg ctgtccaata tgcgcctttg ctctaacgtt ttggcccaac aagtcgtaca
     9961 aacttccttg ggggggcacc aatcagtcga tgaattgctt cttcctggtg gacgaatcta
    10021 cgagcaaaga aatttcatct ataatgccat tcaagatatt ccaggtttgt ctgccgttaa
    10081 acccaaggcg gggctctata tcttcccaaa aatcgaccgc aatatgtacc gtatcgatga
    10141 tgatgagcag tttgtccttg atttcttgaa gcaggaaaag gttctcttgg ttcatggtcg
    10201 aggctttaac tggcaggagc cagaccactt ccgtatcgtt taccttcctc gtgttgatga
    10261 gttagcccaa atccaagaaa agatgactcg tttcttgaaa cagtatcgta gatagggctt
    10321 gcattcgaaa aagctggaaa catttgccta gagctattga caaaagtgaa agaatcagat
    10381 agaatagtaa agtatgttta gtaactgatc actttatagc cggctaaacg aatacaaaat
    10441 ctatgaggag gtattcatcg tgaaacgtac ttatcaacca agtaaacttc gtcgtgcgcg
    10501 caaacacgga ttccgtaacc gtatgtcaac taaaaatggt cgtcgcgtat tggcagctcg
    10561 tcgtcgtaaa ggacgcaaag ttttggctgc ataatccaaa cgaattctat caaaaatcag
    10621 taggaactcg agtctactga tttttatttt tgtaaaaaag ttcagtagat gcaaatggat
    10681 tcggaagcga tgttacagta gattgaaact agaatagtac acctctgttt ctaaaacatt
    10741 gttagaaatc gatttgactg tcctgatcga tttgtcctgt tattatttta ttttactata
    10801 aagttgaagt aggtggagat ggtacagcaa caatcacatt cccagatggt tcagtagtga
    10861 cgattctagg aaaagataca gttcaacaat ctgcgaaagg tgaatctgta actcaagaag
    10921 ctacaccaga gtataagcta gaaactacac caggtggaga taagggaggc aatactggaa
    10981 gctcagatgc taatgcgaat gaaggcggtg gtagccaggc gggtggatca gctcacacag
    11041 gttcacaaaa ctcagctcaa tcacaagctt ctaagcaatt agctactgaa aaagaatcag
    11101 ctaaaaatgc cattgaaaaa gcagccaaga acaagcagga tgaaatcaaa ggcgcaccgc
    11161 tttctgataa agaaaaagca gaacttttag caagagtgga agcagaaaaa caagcagctc
    11221 tcaaagagat tgaaaatgcg aaaactatgg aagatgtgaa ggaagcagaa acgattggag
    11281 tgcaagccat tgccatggtt acagttccta agagactagt ggctcctaat gctgctccta
    11341 agacaacaag tgcaccgcaa gcaactgcag gaacaatgca aaatgttacc taccagtcac
    11401 ctgctggcaa acaattacct aacacaggtt cagcatcaag tgcagcactt gctagtcttg
    11461 gtctagtggt ggcaacaagt ggttttgctt tgctaggaag aaagactaga cgtagaaaat
    11521 agaacagcta gaaaattcta ttctctactt aaagttagat tataaggggg attttgagaa
    11581 gtcatcaatc ctagtgatgg gtgagaaaag tgagaaccca agataatcac atactttagc
    11641 tgaataggaa tattctatca atgtagccaa tctcttctgt ctctaactgt ggaataggag
    11701 ataggcaata tcggatagaa aagatagcag aatagctctc tattgaagag aggaggggaa
    11761 accgaaaaat taggtgcccc tcctcttttt tggtataata gaagatagaa aacgaggtta
    11821 gaagagatga tttttgatac acatacacac ttgaatgtag aagaatttgc aggtcgtgag
    11881 gcagaagaaa ttgccttggc tgctgagatg ggtgtgatac agatgaatat tgttggtttt
    11941 gataaaccga cgattgagcg tgccttggag ttggtagatg agtatgagca gctctatgcg
    12001 actattggtt ggcatcctac agaagctggt acttatacag aggaagttga ggcttacttg
    12061 ttggataagt taaaacatcc caaggttgtg gctttaggtg aaattggctt agactaccat
    12121 tggatgacag cgcccaaaga ggtgcaggag caggtttttc gccgtcagat tcagctatct
    12181 aaggacttgg atttgccttt tgttgtccat acccgtgatg cgctggaaga tacctatgag
    12241 attatcaaga gtgagggcgt tggtcctcgt ggtggtatca tgcattcatt ttcagggacg
    12301 cttgagtggg cagagaagtt tgtggatctt ggtatgatca tttccttctc aggagtggtg
    12361 actttcaaga aggcaactga cctccaagaa gcagctaaag agttaccttt ggacaagatg
    12421 ttggtggaaa cagatgcgcc ttacttagca cctgtaccca agcgtggtcg tgaaaataaa
    12481 acagcctata ctcgctatgt ggtcgacttt atcgctgact tgcgtggtat gacgacagaa
    12541 gagctggcgg tagcaacgac tgcaaatgca gaacgaattt ttggattgga cagcaagtaa
    12601 tgaaagagaa aatttctcaa gttatcgtgg ttgaaggtcg ctatgatacg gtcaatctca
    12661 agcgttattt cgatgtggag acctatgaga cacgaggttc tgccatcaat gatcaggata
    12721 tagagcgaat tcagcgtcta caccaacgtc atggagtcat tgtctttaca gacccagatt
    12781 ttaatgggga acggattcgg cgcatgatca tgacggtcat tccaacagtt cagcatgcct
    12841 ttctcaagcg agatgaagct gttcccaagt ccaagaccaa ggggcgttct ctgggaattg
    12901 agcatgccag ctatgaagac ctgaaaacgg ctctagctca ggtgacagaa caatttgaac
    12961 atgagagtca gtttgacatt agtcgtagcg atttgattcg ccttggtttt ctagcagggg
    13021 cagacagccg taagcgtaga gaatatctcg gagagactct ccgaatcggc tattccaacg
    13081 gcaagcaact cctcaaacgc ctagagttgt ttggggttac tttggcagaa gtggaagaag
    13141 ctatgaaatc ttatgagtag gaaagatgta gccgttacaa ttttttaagt ttcacagtat
    13201 ttttcgaagc aggtagaaga ggaggcgtct gatgttaatt ggtcaaaaaa ttaaagagat
    13261 tcggatagaa aaaggaatta gtcgtccaga tttttgtgga gatgagcaag aactgacagt
    13321 tcgtcaactg tcgcgaattg aaagtggagc ttcgcaaccg agtttgccca agttagacta
    13381 tattgctcgc cggctaggag ttccagttta tagccttatg ccggattttt cagctcttcc
    13441 tcctgcttat ttagaattga aataccagat tttacgtgaa ccaatctatg gtaaagaaga
    13501 ggagtacgat aagaaggaag cgtgtttgga agagatttat aaaacatact ttgataatct
    13561 tcctaaagaa gaacaattag catgtgaagt attgcaggcg tgtttggata cttctagaac
    13621 tagaaggcct gaatatgcag agttaatact tgaggaacat atgtctcaga tcatagaaaa
    13681 agaagtttat tcaacaaatg atatgttgtt gattcgtttg tttttttatc aaatgctcat
    13741 tagaaaagat cttgccaaat ttataaatca aatcgaaaag ctaatgctct ttcttttgga
    13801 acagaaaaag gtaactaaat tagaaaactt ctttataatt agagatactc ttatttcagg
    13861 aatgtgttgt cttgaaaagg taggagtaac tgattgtttt aatgattatc tatcgtgttt
    13921 acaagaaatt atggataaaa ctcaagatta tcaaaagaaa cctcttgtat ttatgttttt
    13981 gtggaagcaa gcattaagag aagaaagaga ttttagttta gctgaatcat tttatcagtc
    14041 ttctaaaaca tttgcgaagc taattggaga tggatttcta gtaaagaaat tgacagagga
    14101 atggcaagag gatgtcaaaa aatatttata aacatagtga atcagtgaca aaaatgtcct
    14161 tgtcctcgta tcaaaacagt tctaaagttc gtctttagaa ctgttttttt agatataagc
    14221 taaaaataat atgaaatagt tagattttaa ggacattgat gtccttgata gtgtgataaa
    14281 gatggagtat aatgactata aaagttcatt tcagggtgct attttgacaa tttagagaaa
    14341 aacaaataat tttagaatat catttggtaa tataatagta tcaacgttga taatggaaag
    14401 aggagcaagt aactcaacaa tcttgtaatc aaaaatagag gagtatcaat atgaaacaaa
    14461 cagttaaaaa gttagctctt gtagcgagca ttgcagcaac attaggtgga ggtgtatcag
    14521 ttgcctctgc agccgttcaa tacccagaag gtggtgtttg gacttatggt tcaggtaacg
    14581 gtggtgctta ctcaaactac tatcaccctt caaaatacca tagttcaaca gttgttagca
    14641 gaaaaactgg ttcatctgac aagggatatg ctggtgctgg agggacttct cgtgcatgga
    14701 ttcgtacttc ttggggagag aaagttgcat tctactataa tgtttagaat ggtatagccc
    14761 tattgtttag ctttacttgg tcaccttctc actaagtgac caagtaactt tttggaggaa
    14821 atgatgaaac gtttatttat tttgatttca atggtattgg tatcgcttta tatggtgata
    14881 acttccgttg accatcgaga agagatttta tttggtaact atccttctgt tgatgtgaca
    14941 ggaatgatga taaatcaacc agtagctagt cgcgaagagg tgacagaggc tttgagtcac
    15001 ttggcggtag agcataatag tctcatcgcc cgtcgaatcg ttgagccaaa tgaagctgga
    15061 gaaacactct ttacctatgc cacttatggt gagggagagc ttccagaagg tctgaccatt
    15121 tcctccaagg agagtgcaga aacgagtgat ttattagggt cttacttgat tgtgtcagga
    15181 agtttggatg gagtgagctt acagaccacc ttgaaagaac ttggctatca aggctttgtt
    15241 tcgaatggag aagatccatt ttcgatagtc ttactattga cggccacccc tatggtgcta
    15301 ctgagtttag ctatttttct gctgaccttt atgagtctga ccctgattta tcggatcaaa
    15361 tcccttcgtc aggcagggat tcgcttaata gctggtgaga gcttgtttgg agttgctctc
    15421 agaccagtgt tagaagatgt gagacagctt atctgctcag tgctggtatc cagtcttttg
    15481 ggattgggga ttctctggta tcaaggtgcc ttgtttatgg caacggtgca actggtcatc
    15541 attgctcttc tactttatgg attgaccctg gcagggattt ctaccttact aagtgtcgtc
    15601 tatctacttg gtttacagga aaatagtctg gtggatctat tgaaagggaa actccctctc
    15661 aaacgtatga tgacattgat gatggtgggg caactcttag ctgtattggt ggtcggatcg
    15721 agtgcgacag ctctcctacc ccactaccgt gaaatgcagg aaatggagag agctagcaat
    15781 aaatggagcc agtcctcaga ccgttaccgt ctatcctttg gttggtctag tgcatttgcc
    15841 gatgaagaag gaacgcgtaa ggataatcgt gagtggcaga catttactga agaacggtta
    15901 gccaatacag actcttttta tattatgagc aatgttgaca atttctcaga tggagcagaa
    15961 gtggacctag atggcaatcg tctcagtgac tacacaccgt cagggaatgt tatctatgtc
    16021 tcaccacgct atctgataga agaaaagatt accgtttctt cagagtttat ggacaagatg
    16081 caaaacttgt ctgagggaga gtttgggctg atcttgcctg agagcttgcg agagcagtct
    16141 acctactacc aaggattgtt tacagattac ctgcaaaact tttcatctga aagtgtagaa
    16201 gtgacgagtc agaaatacta cctcccacag gtaaggctag cttttacaga aacaggacag
    16261 gaacgtttcc tctataatga tgggtacaag acaacacgcc agtacctaaa agatccgatt
    16321 attgtagttc taacgccgca agcgactgga acaagacctg ttgcagggat gttgtgggga
    16381 actacggcta atagtgcctt gaaactagat cgatatggag acagcatcac agctctaaaa
    16441 gagcaaggtc tgtatcacaa ggtttcttac ttggtaaaaa gccagctatt ttttgccaag
    16501 gtactaaatg acaaacgggt ggagttttac tctctcctta ttgggacgat tttgaccctg
    16561 tctacggcta tcttgttatt tgattccatg aatcttctct attttgagca gttcagacgg
    16621 gaacttatga ttaaacgtct tgctggtatg acaatctatg agcttcatgg caagtattta
    16681 ctggcgcaag gaggagttct cttgcttggc ctagtcctat ctagtatttt gacaagagat
    16741 tgtttgatta gcgctctagt tgtagctttg tttacgctta acgccctctt gattttagta
    16801 aggcaggaca aaaaagaaga agctggtagc atggcagtat tgaaaggaaa ataagatgat
    16861 tgatattcaa ggattggaaa agaaatttaa tgaccgcgcg attttctctg gtttgaatct
    16921 caagctggag aagggcaagg tttatgcctt aatcggaaag agtggaagcg gaaagacgac
    16981 gctgctgaat atcttgggaa agctagaaaa gatagatggt ggaagggttc tctatcaggg
    17041 gaaagattta aaaaccattc ccactcgtga gtattttcga gaccagatgg gctatctctt
    17101 tcaaaatttc ggcctcttag aaaaccaatc aatcaaagaa aatttggatt tgggttttgt
    17161 tggtcagaaa atctcaaaag tagaacgttt ggaaaggcaa gtgggggctt tagaaaaagt
    17221 taatctaggg tatttggatt tagaacaaaa aatctatact ttatctgggg gagaggccca
    17281 acgagttgcc cttgctaaga ctattttgaa aaatccaccc ttgattttgg cagatgaacc
    17341 aacagcagct cttgatcctg aaaattcaga ggaggttatg aatctcttgg tggatttgaa
    17401 agatgaaaat cgaattatca tcattgcgac ccataatccc ctagtctgga ataaggctga
    17461 tgaaatcatt gatatgagga aacttgctca tgtgtgaaaa aatccgtatt cgcagggtat
    17521 ctgattatcc tagtgccaga ggtggtttag aagatatcct catcatggaa aatatgacca
    17581 atcatctcct tttggttcaa atccgagtgc atggctattt gcttgatttt gctagtattg
    17641 aagggcaaag gcaaaagcat tatcgtttga aaaatttacc tcagacggtt gaactgacag
    17701 tggatgatgt ggaggaggat gtggatttga ccctacccga aaatcgaagt tatcaagaag
    17761 ctgatttttt tgatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaggctg
    17821 tacttgagta cggcaaggtg aagctgacgt ggtttgaaga gattttcgaa gagtatgaac
    17881 gtatgtttcg agagaagcac taagtccctc tttaaagatt tccaagacta tctttcttca
    17941 tgaggaaaga tagttttttg gtatgatttt cattcccaaa atacaagggg aatgtgttac
    18001 aatagtagta acagataata gaaaagagaa tagatgagaa ttgcagatta tagcgtgacc
    18061 aaggcagtgt tggagcgtca cggttttacc tttaaaaagt cctttgggca aaatttcttg
    18121 acggatacca atatccttca aaaaattgtg gatacggctg aaattgatga tcaggtcaat
    18181 gtcatcgaaa tcgggccagg tattggtgcc ttgacagaat ttttggctga gcgtgcagcc
    18241 caagtcatgg cttttgagat tgaccaccgt ttgatgccaa ttttggcaga tactctgcgt
    18301 gattttgata atgtgaccgt agttaacgaa gatattctca aggttgattt ggcgcaacat
    18361 atccaaaatt ttaaaaatcc taacctgcca atcaaggtag tggctaattt gccttactac
    18421 atcacgacgc ctattctcat gcacttgatt gagagtggca ttcctttttg tgagtttgtg
    18481 gtcatgatgc agaaagaagt agcggaccgc atttcagccc agcctaacac caaggcttac
    18541 ggtagcttgt ctatcgccgt gcagtattac atgacagcca aggttgcctt tatcgtgcct
    18601 cgtacggtct ttgtgccagc gccaaatgtg gattcagcca tcttgaaaat ggtgcgtcgt
    18661 ccagagccag ccgtagcagt agaagatgag aactttttct ttaaggtttc caaggctagt
    18721 tttacccatc gccgcaagac cttgtggaat aacttgacag gttactttgg taagactgaa
    18781 gaggtcaagg acaagctgac caaggctttg gaccaggcag gcttgtcacc aagtgtgcgt
    18841 ggggaagctc tcagcttggc agaatttgcc ggtctagcag acgcacttaa agggcaagga
    18901 ctctaagatg cagggacaaa tcattaaagc cttggcaggt ttctactatg tggagagtga
    18961 tggccaggtt tatcaaacac gcgcgcgtgg gaatttccgt aaaaaaggcc atacccctta
    19021 tgttggggac tgggtagatt tctctgccga ggaaaattca gaaggctata tcctcaaaat
    19081 tcacgaacgg aaaaacagtc tggttcgtcc gcctattgtc aatatcgatc aagctgtagt
    19141 aatcatgtcc gtcaaggaac ctgattttaa cagcaatttg ctggatcgtt tcttggttct
    19201 tttggagcac aagggcatcc atcccattgt ctatatttcc aaaatggatt tgttggaaga
    19261 taggggagaa ctggattttt accagcagac ctatggtgac atcggctatg actttgtgac
    19321 cagtaaagag gaactcctgt ctttgttaac aggcaaggtt acggtcttta tggggcagac
    19381 aggtgttggg aagtcaactc ttctcaataa aatcgcacca gacctcaatc ttgaaacggg
    19441 agaaatttca gacagtctag gtcgcggtcg ccataccact cgagctgtta gtttttacaa
    19501 tctcaacggg ggtaaaatcg cagatacacc aggcttttca tctctggact atgaagtatc
    19561 aagggctgaa gacctcaatc aggcctttcc agagattgct actgttagcc gagattgtaa
    19621 gttccgtact tgtacccata cccatgagcc gtcttgtgcc gtcaaaccag ctgttgaaga
    19681 gggtgttatt gcaaccttcc gttttgacaa ttacctgcaa ttccttagtg aaattgaaaa
    19741 tcgtagagaa acctataaaa aagtcagcaa aaaaattcca aaataaggag aaacctatgt
    19801 ctcaatacaa gattgctccg tcaattctgg cagcagatta tgccaacttt gaacgtgaaa
    19861 tcaaacgtct agaagcaact ggggcagaat atgcccatat cgatatcatg gacagtcatt
    19921 ttgtaccgca aatcagtttt ggtgcaggtg tggtcgagag ccttcgtcct catagtaaga
    19981 tggttttcga ttgccacttg atggtgtcaa accctgagca tcatctggaa gattttgcgc
    20041 gtgcaggtgc agacatcatc agtatccatg tagaagcaac gcctcatatt catggcgccc
    20101 tccaaaaaat tcgttcactc ggagttaagc cttcagtcgt tatcaatcct ggcacaccag
    20161 ttgaagccat caagcacgtc cttcatctag ttgaccaagt tttagtcatg acggttaatc
    20221 caggttttgg tgggcaagcc tttctgccag aaaccatgga taaggttcgt gagttggttg
    20281 ctcttcgtga ggaaaaaggt ttgaactttg aaatcgaagt ggatggtggg attgatgacc
    20341 aaactattgc tcaagccaaa gaagctggtg cgactgtttt tgtagcaggt tcctatgtct
    20401 ttaagggaga agtcaatgag cgagtacaaa ctctcagaaa acaactggac tagggttgca
    20461 gtttttgcag gcggaaaccg cggtcattat cggacagatt ttgatgcttt tgttggggtg
    20521 gatcgaggct cgctctgggt cttggaagaa gacttacctc ttgctctagc agtcggagat
    20581 tttgattctg tgacggaaga agagcgacag gtgattcaaa aacgtgccca gtattttgtc
    20641 caagcccgac cagaaaaaga tgataccgat ttggaattgg ctctcttaac catctttgag
    20701 aaaaatcctc aggctcaggt tactattttc ggtgccttgg gtggccgtat tgaccatatg
    20761 ttggccaatg tctttctgcc tagcaatcct aagttggcac cctatatgca tcaaatagaa
    20821 attgaggatg ggcaaaactt gattacttat tgtccagaag ggaccagtca gctagaacct
    20881 cgttcagact acgactatct agcctttatg ccagttcggg atagccagct gactattctt
    20941 ggagccaagt atgagttgac agaggaaaat tttttcttta aaaaagtgta cgcttctaac
    21001 gaatatatag atagggaagt gtcggtaact tgcccagatg gttatgtggt cgtactgcat
    21061 agcaaggata ggaggtagga tggaaagttt acttattcta ttattaattg ccaacctagc
    21121 tggtctcttt ctgatttggc aaaggcagga taggcaggag aaacacttaa gtaagagctt
    21181 ggaggatcag gcagatcatt tgtcagacca gttggattac cgctttgacc aagccagaca
    21241 agccagccag ttagaccaaa aagatttgga agtggctgtc agcgaccgtt tgcaagaagt
    21301 gcggattgaa ttgcaccaag gcctgaccca agtccgtcaa gaaatgacag ataatctcct
    21361 ccaaactaga gataagacag accaacgtct ccaagccttg caggaatcaa atgagcaacg
    21421 tttggaacaa atgcgccaga cggtcgagga aaaactagaa aagaccttgc agacacgctt
    21481 acaggcttcc tttgagacag tttctaaaca actggagtct gtcaatcgtg gtcttggaga
    21541 aatgcagaca gttgcccgtg atgtcggagc tcttaacaag gttctctctg gaaccaagac
    21601 gcgagggatt ctgggagaat tgcaactggg gcaaattatt gaagacatca tgacacctgc
    21661 ccagtacgaa cgagaatacg caacggttga aaactctagt gaacgagtgg agtatgccat
    21721 caagttaccc ggacaaggcg accaagaata cgtctatctt ccaattgact ctaagtttcc
    21781 actggcagat tattaccgct tggaagaagc ctatgagaca ggtgacaagg atgagattga
    21841 acgctgtcgt aagtcactcc tagcaagcgt caagcgcttt gccaaggata tcaagagcaa
    21901 gtacatagca ccgcctcgga caaccaactt tggaatcttg tttgttccga cagaaggtct
    21961 ttattcagag attgtgcgca atccggtctt ctttgatgat ttgagacggg aagaacagat
    22021 tattgttgca ggaccaagta ccctatcagc ccttcttaac tccctatcag ttggtttcaa
    22081 gacccttaat atccaaaaga gtgccgacca tatcagcaag actcttgcca gtgtcaagac
    22141 cgagtttggc aagtttggtg gtattctggt caaggcacaa aaacatctcc aacatgcctc
    22201 tggcaatatt gatgaattat taaaccgtcg taccatagct atcgagcgga cgctccgtca
    22261 cattgagttg tcagaaggtg agcctgcgct tgatctactc tattttcaag aaaatgagga
    22321 agaatatgaa gattagtcac atgaaaaaag atgagttatt tgaaggcttt tacctaatca
    22381 aatcagctga cctgaggcaa actcgagctg ggaaaaacta cctagccttt accttccaag
    22441 atgatagtgg cgagattgat gggaagctct gggatgccca acctcataac attgaggcct
    22501 ttaccgcagg taaggttgtc cacatgaaag gacgccgaga agtttataac aatacccctc
    22561 aagtcaatca aattactctc cgcctgcctc aagctggtga acccaatgac ccagctgatt
    22621 tcaaggtcaa gtcaccagtt gatgtcaagg aaattcgtga ctacatgtcg caaatgattt
    22681 tcaaaattga aaatcctgtc tggcaacgga ttgtccgaaa tctctacacc aagtatgata
    22741 aggaattcta ctcctatcca gccgccaaga ccaaccacca tgcctttgaa acgggcttgg
    22801 cttatcatac ggcgaccatg gtacgtttgg cagacgctat tagcgaagtt tatcctcagc
    22861 tcaataagag cttactctat gcggggatta tgctgcatga cttagctaag gttatcgagt
    22921 tgacggggcc agaccagaca gagtacacag tgcgaggtaa tcttcttgga catatcgctc
    22981 tcattgatag cgaaattacc aagacagtta tggaactcgg catcgatgat accaaggaag
    23041 aagtcgtttt gcttcgtcat gtcatcctca gtcaccacgg cttgcttgaa tatggaagtc
    23101 cagtccgtcc acgtattatg gaggcagaga ttatccatat gattgacaat ctggatgcaa
    23161 gcatgatgat gatgtcaaca gctctggctt tggtggataa aggagagatg accaataaaa
    23221 tcttcgctat ggataatcgt tccttctata aaccagattt agattgataa tttaagaaaa
    23281 atgagcattt tttaggataa gaatgttcgt ttttttatgt gaatatggta taataagtaa
    23341 aagacaaaaa tgaatactct tcgaaaatct cttcaaacta gggtagtatc gccttgtcgt
    23401 atgtatatat gcaggtatat tacagggttt gtcagttcta tcgacaatct caaagcagtg
    23461 ttttgaacca ccagcgacca gctttctagt ttgctttttg attttttgaa taaaaatgga
    23521 ataggaaata gaaatgaaat taagaagaag tgatcggatg gttgtcattt ccaactattt
    23581 gattaataat ccttataaac taactagtct caatactttt gctgaaaagt atgagtctgc
    23641 taaatcatcc atctcagaag atatcgtcat tatcaaacgc gcctttgagg aaattgaaat
    23701 cggtcatatc cagacagtga ctggggctgg cggaggtgtc atcttcacac cgtctatttc
    23761 gagtcaggat gctaaggaaa tggttgaaga cttgcgtacc aagttgtcag aaagtgaccg
    23821 tatcttgcca ggtggttata tctatctgtc tgatttgctt agcacaccag ccatcttgaa
    23881 aaatattggt cgtattattg ccaaaagctt tatggaccaa aaaattgacg cggttatgac
    23941 cgtagcaact aagggtgtgc cacttgcaaa tgcagttgcc aatgtcctca atgtctcttt
    24001 tgtcattgtg cgccgtgacc tgaaaattac cgaaggttca actgttagcg tcaactatgt
    24061 ttcaggttca agtggtgacc gtatcgagaa aatgttcctt tcaaaacgta gtcttaaggc
    24121 aggtagccgt gtcttgattg tggatgactt cttgaaaggt ggcggaacgg tcaacggtat
    24181 gattagtctc ttgcgcgagt tcgactcaga actggcaggt gtagcggtct ttgcggacaa
    24241 tgcccaagaa gaacgtgaaa agcagtttga ctacaagtca ctcttgaagg taactaatat
    24301 tgatgtcaag aaccaagcca tcgatgttga ggttggcaat atctttgacg aagataaata
    24361 agagatagaa ctaaaggttg gaacgattgt cccagccttt ctttgcaaac agaatagaag
    24421 gaagcttatg aaaacaccat ttatcaatag agaagagtta gaagcgattg ttgccgagtt
    24481 cccgactccc tttcacttgt atgatgagaa ggggattcgt gagaaggcaa gagccgtcaa
    24541 ccaagctttt tcgtggaaca agggctttaa ggaatatttt gcagttaagg ctactccaac
    24601 tccagctatt ttgaaaattc tccaagaaga aggttgtggt gtggactgct ctagttatgt
    24661 agagcttttg atgagccata aactggactt tctgggttct gagattatgt tctcttccaa
    24721 caacacgcca gacaaggaat acgcctatgc acgtgaattg ggtgcgacca ttaacttgga
    24781 tgcctttgaa gatattgaac atctggagag agtagcaggc attccagaaa tcatctcttg
    24841 tcgttataat cctggaggcg tttttgaact ggggacagac attatggaca atcctgggga
    24901 ggctaagttt ggcatgacca aggaccagct ctttgaagcc tttgctatct tgaaggaaaa
    24961 aggagccaag acttttggga ttcactcctt cctagcgtcc aataccgtga cccatctcta
    25021 ttatccagag ttggctcgtc agctctttga actggctgtt gaaatcaagg aaaagttggg
    25081 catttggcta gactttatca atctttctgg cggtattggt gttaattatc gtccagacca
    25141 ggagccgaac gatatcgcct tgattggtga gggagttcgt aaggtgtatg aagaagttct
    25201 tacgtcagca ggtcttggtc aggtcaagat tttcaccgaa ttgggtcgtt ttatgctggc
    25261 acctcacggt gctctagtca caagagtgac tcataagaag gaaacctacc gtacctatct
    25321 aggtgtggat gcctcagcag tcaacctcat gcgtccagct atgtacggag cttaccatca
    25381 tattagcaac gtgacccatc cagatggacc agctgaagcg gtagatgtgg ttggttcact
    25441 ctgtgaaaac aatgataaat ttgcagtcaa tcgcgaactg cctcatacag aaatcggtga
    25501 tttgctggta attcatgata caggtgccca cggattttca atgggctacc agtataatgc
    25561 caaattacgt tctgcggaaa tcctctatac cgaagaaggt aaagcccgtc aaatccgccg
    25621 tgcagagcgc cctgaggact attttgcaac cttatatggc ttcgattttg aagaataatc
    25681 tgataataga ttgaaaatga aattgaaaaa cagattgctt tctaaaaaat aggcaaaaat
    25741 cttgtttttc cttcaagtcg tgatataata aaactataaa acgttttcaa ggaaggtaac
    25801 gatatgtctg aagaaacaat tgattatgga caagtgacag gaatggtgca ttcgacagaa
    25861 agctttgggt cagtagatgg gcctggtatt cgctttattg tctttttgca gggctgtcac
    25921 atgcgttgcc agtattgcca caacccagac acttgggcta tggagtccaa taagtcacgt
    25981 gaacggacgg tagatgatgt cttgacagag gccttgcgct accgtggttt ctggggaaat
    26041 aagggtggga ttacagtcag tggaggagaa gctctcttgc agattgattt cctgattgct
    26101 ctcttcacca aggctaagga acaaggaatc cactgtacct tggatacctg tgctcttcct
    26161 ttccgtaata aaccacgtta ccttgagaag tttgacaaac tcatggctgt cactgacttg
    26221 gttcttttgg atatcaagga aatcaacgaa gaacagcaca agattgtcac tagacaaacc
    26281 aataaaaata tcttggcttg tgcccagtat ctatcagata ttggaaaacc tgtctggatt
    26341 cgccacgtgc tagttccagg attgacagac agagatgatg acttgattga acttggtaag
    26401 ttcgtcaaga ccctcaaaaa tgttgataag tttgaaattc taccttatca caccatgggt
    26461 gagttcaagt ggcgtgaact tggaattcca tattccctcg aaggagtcaa accaccaaca
    26521 gcagatcgcg tcaagaacgc taaaaaactc atggataccg aaagttatca agattatatg
    26581 aaacgtgtac atggatagaa aagaagcctg atggaaacat cgggcttttg atttgcaaaa
    26641 agacttagca aatcagctaa gcctttttct tcttatctcg aacgttgttt tccagcgttg
    26701 cgatttttgt gttttttctt gcttgtgata gcagttggtt gttcaggggt aacgtctttt
    26761 cgtccacttg gtttagagaa agcacttgct tttggtgggt tcttggctag ttcttcacgg
    26821 acttttttgc gaagttttgg acgaacgata tagttgacga taaactgttg gagaatcatc
    26881 atgaaaccac cgacaaccca gtaaagtgtg acactagctg gtgagaagag ggagaagacg
    26941 acgatcatga gtgggctcat gtaaatcatt ttcttgattt gttctctttg catttcatct
    27001 tctactccgt gaagtgaaag gagcgattga agatagtaaa ggacaccagc acaggcaacc
    27061 aaaatcatac ttggagaacc tagaggaatg cctaggtagc ttgcttgagc aaccccttca
    27121 gtatgttggg cagcaaagta gatagcagag aagaaaggca tttgaaggag gatagggaaa
    27181 catcctacac cgccaaacat gctgataccg tgctcttttt gagcagcaaa gagagcttgt
    27241 tgggcttcga gtttttcttc ttgagtagtc gcttctttga gacgcgtttg gtgtggctca
    27301 aggacgtgct tgagggcgtt catcttttca gagtgaagcg ttgccttcca tgattggtag
    27361 ataccaagtg gcaagataat caagcgcacg ataatggtta cgatgatgat agcgacacca
    27421 aagcctagac ctttatcagt agcgaagtac ttgatagctt cagccatagg ggctccgatg
    27481 gtattccaaa taaatcctgt tggctgacct gtggttttat cgacattgac acaaccagtc
    27541 aagacaagca acatagccgc tcccatagct gagagtgcaa aacgtttaat agatttcact
    27601 gtttttattc cttctttaaa aattatacct ttctattcta ctgttttttt acaaaatata
    27661 caatagttct ggagacctaa tttgcgattt taaagtcaga gtagggagga aagttgctta
    27721 gtttgacatc taagtagctt acttttgaaa aaggtgtcgg tccttttcgg atttcttgga
    27781 taaattttgc catgatagca gatgagtctg cttgggctaa gatttccact gtgccatcgt
    27841 cgttattcca tactcgacct gtgatgccac caatttcaag tgccaagctg taaacacccc
    27901 aacgaaagcc gactccctgt accctacctt gggcaatcat tctaaccttt tgcataccaa
    27961 acccctttga ttgtgttata atatttctat gactattata acctcaaaag ccaattctgt
    28021 ggtaaaaaat gccaagaaat tacgccaaaa aaaataccgc aagtctgcct acttgattga
    28081 aggctggcac ttgtttgaag aagctgtcca agctggagtg acgattgaga aaatctttgc
    28141 cctagaaaat tatcgagatc agttagctac tttttcgcaa actgtctggg tgtcagagga
    28201 tattttgcta gatttggcgg attctcagac tccacaggga attgttgccg tggttcaaaa
    28261 agaagaagta ggacaagctg atttgagtca gggcaagttc ttgtttttgg aagatgtgca
    28321 agatcctggt aatgtaggaa ctatcattcg aactgcggat gcagcaggtt ttacaggagt
    28381 gattgtttca gataagtcgg cagacatcta cagtctcaaa accctacgtt ccatgcaagg
    28441 tagtcatttc catctgccca tttaccggat gactagtcaa gcgcttcttg acgaaactaa
    28501 aaaggtagct atcccagtgc tagcaacaac cctatctaaa gattctgttg attacagaga
    28561 actgccttct atagaaaatt ttgtactagt tatgggaaat gagggtcaag gaattagtcc
    28621 ccttatggct gaaagtgcag accagttggt ccatattagc atgaaggggc aggccgagag
    28681 tttgaatgtt gcggttgcag ccggtatttt aatcttccat ttaagctaat tttaactttt
    28741 tttgttataa tcaaggaaag atgttcacag aaaaggagaa atggatgaat cacactatta
    28801 tacatgaccg tgcaggtctc aatcaatttt acgctaaggt ttatgccttt gttggtctgg
    28861 gaatcggact atctgctttg gtatcaggcc ttatgttgac ggtctttcag tctcagttgg
    28921 tttacttttt gatgcagggg cgtctctggt tgaccattgc tacttttgcg gagttggctt
    28981 tggttttcgt tgccagtagc atggcttcaa gaaatagtcc agctgctctt ccagtatttt
    29041 tactttactc cgttttaaac ggctttaccc ttagttttgt ggtggctttc tatactccgg
    29101 gtacagtttt atctgccttt gtatcgagcg cccttctctt ctttgtcatg gcggcagttg
    29161 gtatttttac caagaaagac ttgagtggga ttggtcgagc aatgatggca gctctcattg
    29221 gtttactgat tgctatggta gtcaatattt tcttggctag tggcttcttt gattatatga
    29281 ttagcgtagc catggttttg gttttttcag ggttgattgc ttgggataac caaaagattc
    29341 gcctcgccta tgaacaatca caaggtcgtg tagcgacagg ctgggtggtg tcaatggctc
    29401 tcagcatcta tcttgatttt atcaatcttt tcctaagtat tctacgtatc tttggtcgca
    29461 atgactaaaa ggtctatgaa atcagtgttt tgagtgacac tgattttttt taaattttct
    29521 cttttctttt cttggaaata ggtgtataat acgtttatta aatttttgag gagttgtcta
    29581 tgaagaaaag ttttatccat caacaagaag aaatttcctt tgtcaaaaac acttttaccc
    29641 agtatttgaa agataagcta gaagttgtcg aagttcaagg tcctatcttg agtaaggtcg
    29701 gtgacggaat gcaggacaac ctgtctggtg tggaaaatcc agtatcggtc aaggttctcc
    29761 aaatccctga tgctacttat gaagtggtgc actcacttgc taaatggaaa cgccacacct
    29821 tggctcgttt tggctttggt gaaggagagg gtctctttgt ccacatgaaa gcccttcgtc
    29881 cagatgagga ttccttggat gcaacccact ctgtttatgt tgaccagtgg gactgggaga
    29941 aggttatccc aaatggtaag cgtaacatcg tttatctaaa agaaacagtt gagaagattt
    30001 ataaggctat tcgcctgact gagctagctg ttgaagcccg ctatgacatc gagtctatct
    30061 tgccaaaaca aatcaccttt atccatacag aagaattggt agaacgctac ccagacttga
    30121 cacctaaaga acgtgaaaat gcgatttgta aagaatttgg agccgtcttt ttgattggta
    30181 tcggtggcga gttgccagat ggtaaaccgc acgatggacg tgcaccagac tatgatgact
    30241 ggacaagcga gtctgagaat ggctacaagg gtctaaatgg tgatattctt gtctggaatg
    30301 agtctttagg tggagccttt gagttgtctt ctatgggaat tcgtgtagat gaagaaactc
    30361 ttagacgtca ggtggaaatt acaggtgatg aagaccgctt ggaattggaa tggcacaagt
    30421 ctttgttgaa tggcctattc ccattgacaa tcggtggagg aattggacaa tctcgtatgg
    30481 ccatgttcct acttcgcaag agacacatcg gagaagtgca aacaagtgtt tggcctcaag
    30541 aagtccgcga tacttacgaa aatattttgt agagaatcga accgcaaggt tcggttttct
    30601 ttctcttttt gtctataatt tggtataata aacagtatga aaatcgtatc aggaatctat
    30661 gggggacgtc ccctcaagac actagaaggc aagacaacaa gacctacttc ggataaggtt
    30721 aggggagcca tttttaacat gattggtccc tactttgaag ggggacgagt cttggacctt
    30781 tatgcaggta gtggtggttt atctatcgaa gcagtatcgc gtggcatgtc cagtgctgtt
    30841 ttggtggagc gagaccgtaa ggctcagacc atcgtggctg aaaatatcca gatgaccaag
    30901 gaagttggaa aatttcaact cctcaagatg gatgcagaga gggcattgga acaggtatct
    30961 ggggaatttg acctcgtttt cttagaccct ccctatgcca aggaacaaat cgtagcagat
    31021 attgaaaaaa tggctaagag agagcttttt tctgaagatg ttatggttgt gtgcgagacg
    31081 gataaggccg ttgaacttcc agaagagatt gcctgtctgg gtatctggaa ggaaaagatt
    31141 tatggaatta gtaaggtgac agtctatgtc agataagatt ggcttattca caggctcatt
    31201 tgatccgatg acaaatgggc atctggatat cattgaacgg gcgagcagac tttttgataa
    31261 gctttatgtg ggtatttttt ttaatcccca caaacaagga tttctccctc ttgaaaatcg
    31321 taaacggggg ctagaaaagg ctttgggaca tttggaaaat gttgaagtcg tggcttctca
    31381 tgatgaattg gtggtcgatg ttgcaaaaag actgggggct actttcctag tacgggggtt
    31441 gaggaatgcg gcggatttgc aatatgaagc tagttttgat tactacaatc atcagctgtc
    31501 ttctgatata gagactattt atttacatag ccggcctgaa catctctata tcagttcatc
    31561 aggtgttcga gagcttttga agtttggtca ggatattgcc tgccatgttc ccgagagtat
    31621 tttggaggaa ataagaaatg aaaaaaaaga ttagatggcc cttatacgtc attgcggcct
    31681 tgattgtgac tttcttggca tttgtagtgc ccttgcctta ttatatagag gttccaggtg
    31741 gttcggaaga tattcgccaa gtccttaaag taaatgacac agaagataag gaagctggtg
    31801 cctatcaatt cgttacggtt ggtgttcaac atgccacttt agctcatatg atttatgctt
    31861 ggttgacacc ttttacagat attcgtagtg ctcaggagac tacaggtggt tcttccgatg
    31921 ttgaatttat gcgaatcaat caattctaca tgcaaacatc gcaaaatatg gccaagtatc
    31981 aaggactaaa aacagctggt aaggatatcg aactcaagta ctttggagtt tatgttttga
    32041 atgtgacgga taattcaacc tttaaaggga ttctcaatat ctctgataca gtcacagcag
    32101 tcaatgatca gacctttgat agttccaaag acttgattga ttacgtcagt tctcaaaaat
    32161 taggggattc cgtcaaggtc acctatgaag aggatgggca aaccaagtct gcagaaggaa
    32221 agattatcac cttggaaaat ggcaaaaatg gaattggaat cggcttgatt gaccgtacag
    32281 aggtaatcag caatgtccca attagctttt caacagctgg tattggcggc ccaagtgctg
    32341 gtctcatgtt tagtctagct atctatactc aaatagctca cccagatctt cgtaatggtc
    32401 gtattgttgc cggtacaggt accattgacc gcgatggtaa tgtgggagac attggaggta
    32461 ttgataagaa ggttgtagct tcggctaggg caggtgctgc tattttcttt gctcctgata
    32521 accctgttag cgaagaagaa caaaaggcgc atctggacgc gaaaaacaac taccaaacag
    32581 ccctagaagc ggctaaaaca atcaagacgg atatgaaaat cgtgcccgtt aaaaccctac
    32641 aagatgcgat tgattacttg aaaaacaatc cctaattttt gagtgaagtt tccaaactag
    32701 cgcacaaaca gagaagatgg tataatagtc aaatggttca attattattc actctaagta
    32761 gtcatatgct ctttatttat gtgagttttt accttttaaa ggatcttgtt agatgggaga
    32821 aggttttaaa agtgacagat gataatacaa gaaaagttcg tttattagta gcctttttta
    32881 gcattgtcat aggctacatc ctgagttctt tctttattag cctgtatcat ttgtggcaag
    32941 aagcgcttag aggattatta taaaatcaag agtaaaggaa acgagtatgg ataaaattgt
    33001 ggttcaaggt ggcgataatc gtctggtagg aagcgtgacg atcgagggag caaaaaatgc
    33061 agtcttaccc ttgttggcag cgactattct agcaagtgaa ggaaagaccg tcttgcagaa
    33121 tgttccgatt ttgtcggatg tctttattat gaatcaggta gttggtggtt tgaatgccaa
    33181 ggttgacttt gatgaggaag ctcatcttgt caaggtggat gctactggcg acatcactga
    33241 ggaagcccct tacaagtatg tcagcaagat gcgcgcctcc atcgttgtat tagggccaat
    33301 ccttgcccgt gtgggtcatg ccaaggtatc catgccaggt ggttgtacga ttggtagccg
    33361 tcctattgat cttcatttga aaggtctgga agctatgggg gttaagatta gtcagacagc
    33421 tggttacatc gaagccaagg cagaacgctt gcatggcgct catatctata tggactttcc
    33481 aagtgttggt gcaacgcaga acttgatgat ggcagcgact ctggctgatg gggtgacagt
    33541 gattgagaat gctgcgcgtg agcctgagat tgttgactta gccattctcc ttaatgaaat
    33601 gggagccaag gtaaaaggtg ctggtacaga gactataacc attactggtg ttgagaaact
    33661 tcatggtacg actcacaatg tagtccaaga ccgtatcgaa gcaggaacct ttatggtagc
    33721 tgctgccatg actggtggtg atgtcttgat tcgagacgct gtctgggagc acaaccgtcc
    33781 cttgattgcc aagttacttg aaatgggtgt tgaagtaatt gaagaagacg aaggaattcg
    33841 tgttcgttct caactagaaa atctaaaagc tgttcatgtg aaaaccttgc cccacccagg
    33901 atttccaaca gatatgcagg ctcaatttac agccttgatg acagttgcaa aaggcgaatc
    33961 aaccatggtg gagacagttt tcgaaaatcg tttccaacac ctagaagaga tgcgccgcat
    34021 gggcttgcat tctgagatta tccgtgatac agctcgtatt gttggtggac agcctttgca
    34081 gggagcagaa gttctttcaa ctgaccttcg tgccagtgca gccttgattt tgacaggttt
    34141 ggtagcacag ggagaaactg tggtcggtaa attggttcac ttggatagag gttactacgg
    34201 tttccatgag aagttggcgc agctaggtgc taagattcag cggattgagg caagcgatga
    34261 agatgaataa gaaatcaagc tacgtaatca atcgtttact tttagtcatc atagtactga
    34321 ttttaggtac tctggctcta ggaatcggtc taatggtagg ttatggaatc ttgggcaagg
    34381 gtcaagatcc atgggctatc ctgtctccag caaaatggca ggaattgatt cataaattta
    34441 caggaaatta ggctggagaa ccagcctttt tctaaagata aggagaaata tgaacaaaaa
    34501 aacaagacag acactaatcg gactgctagt gttattgctt ttgtctacag ggagctatta
    34561 tatcaagcag atgcagtcgg cacctaatag tcccaaaacc aatcttagtc agaaaaaaca
    34621 agcgtctgaa gctcctagtc aagcattggc agagagtgtc ttaacagacg cagtcaagag
    34681 tcaaataaag gggagtctgg agtggaatgg ctcaggtgct tttatcgtca atggtaataa
    34741 aacaaatcta gatgccaagg tttcaagtaa gccctacgct gacaataaaa caaagacagt
    34801 gggcaaggaa actgttccaa ccgtagctaa tgccctcttg tctaaggcca ctcgtcagta
    34861 caagaatcgt aaagaaactg ggaatggttc aacttcttgg actcctccag gttggcatca
    34921 ggtcaagaat ctaaagggct cttataccca tgcagtcgat agaggtcatt tgttaggcta
    34981 tgccttaatc ggtggtttgg atggttttga tgcctcaaca agcaatccta aaaacattgc
    35041 tgttcagaca gcctgggcaa atcaggcaca agccgagtat tcgactggtc aaaactacta
    35101 tgaaagcaag gtgcgtaaag ccttggacca aaacaagcgt gtccgttacc gtgtaaccct
    35161 ttactacgct tcaaacgagg atttagttcc ctcagcttca cagattgaag ccaagtcttc
    35221 ggatggagaa ttggaattca atgttctagt tcccaatgtt caaaagggac ttcaactgga
    35281 ttaccgaact ggagaagtaa ctgtaactca gtaaaagata agcctaaact cctatgtcac
    35341 ttatggatgt aggagttctt tttactagtt taagcagggc tagaacaggt actaagaaaa
    35401 aatagtaact tctaaaacta acttccagtt tcccacaacc tagcttttag tatgagaaaa
    35461 acgcgtgaaa tcagtggaaa ttcgtcttag actaacttcc agttttggga gagagatgga
    35521 agttacgcta agaaaaaaat aatatgtatt ggattaaatt ttgtgatata ataaaggata
    35581 agatactatt ttaggagaag aactatggaa gacccaagta gtcagaattt gttgctacaa
    35641 tttgttttat tgtttatctt gacggtatta aatgcctttt tctcagccac agaaatggcc
    35701 atggtttctc ttaatcgtgc ccgagttgaa caaaaggcgg aagaaggaga cagacgctac
    35761 atccgcctgc tgaaggtact agaaaatcct aaccactttt tatcaaccat tcaggtagga
    35821 atcaccctga ttacaatctt atcaggggcg agtttaactg atactctagg acgtgtgatt
    35881 gcctcttggc ttgggaatgg cgaaacagca caagccgtgg caacttttct atccttggca
    35941 tttttgacct atatttccat cgtttttggg gaattatatc ctaagagaat cgctcttaat
    36001 ctaaaggatg ccttggcaat tcgtacagcg ccggttatca ttgggatcgg aaaactagtc
    36061 agtccttttg tttggctttt agctgcttcg accaatttct tgagccattt gactcctatg
    36121 tcattcgatg atgcggatga aaagatgacc cgtgatgaaa ttgcctatat gctgacaaat
    36181 agtgaagaaa cattggatgc tgatgagatt gagatgctac aaggtgtctt ttcgctcgat
    36241 gaactgatgg cacgagaggt tatggttcct cgaacggatg cctttatggt ggatattcag
    36301 gatgatagtc aagccattat ccaaagtatt ttaaaacaaa attattctcg tatcccggtt
    36361 tatgatgggg ataaggacaa tgtaattgga atcattcaca ccaagagtct ccttaaggca
    36421 ggctttgtgg acggttttga caatattgtt tggaagagaa ttttacaaga tccacttttt
    36481 gtacctgaaa ctatttttgt ggatgacttg ctaaaagaac tgcgaaatac ccaaagacaa
    36541 atggcaatct tgctggatga atacggtggt atggctggtt tggtgacttt ggaagacctc
    36601 ttagaggaaa ttgttggtga aattgatgat gaaacggaca aagcagcaat agatgttcat
    36661 cagattggcg aggataccta cattgttcaa ggaactatga ccctcaatga tttcaataat
    36721 tactttaatg ttgaactgga aagtgatgac gttgatacta tcgctggtta ttatttgaca
    36781 ggagtgggaa ccattccaac gactgagaaa ctcagctatg aattgatcag ccaaaacaaa
    36841 cagtttatct taaccaatga taaagtgaag aatggacgtg ttaccaaggt aaaaatccaa
    36901 atcacagaag tcgaaataga agaagaaaca gaataaacta gaggcttttg tcaccaggcg
    36961 tgacgaagct tttttcaatt gatttcaaaa gggaattatg ccatggaaag aactctgcca
    37021 caaactcgct ccaaaagttt ttaaagttat acgtatttat tcgagggaaa ataagaaaag
    37081 cccttcaaat tgggctttct gttcatttga aacctgataa atcaagttat agaaggcggt
    37141 agacggattt gaaccgacga tcaagctttt gcagagccgt gccttaccac ttggctatac
    37201 cgccttaacg tttattatta taccttgaaa atgattttca gtcaatagtt ttttcagttt
    37261 tttgtaccat agcgtgattc gaatgtgatg ccaagttttc tatgggaaaa aatttaccaa
    37321 aaacctactg aaaccccttg aaaaatctag ctaaataggg tataatatgg gtaatcattt
    37381 gtcgtaggtt ttgtctgaaa tattgtccag acaaggctca cagcagttaa atcttctgaa
    37441 aaagtcagat ttagctgctc tttttgtgct ttttttcaag attttgagca tttgtaacag
    37501 aggcttaaag attctgaaaa ttcgtcaaga ggacacggtg ataaggggtt tacaaccata
    37561 tggcgattag aaaagcctga ttgacaaggc ttggaactta tttacaaagg agaatcatct
    37621 tggcaggaca tgacgttcaa tacgggaaac atcgtacccg tcgtagtttt tcaagaatca
    37681 aagaagttct tgacttacca aatttgattg aaattcaaac tgactcattc aaagctttcc
    37741 tagaccacgg tcttaaggaa gtgtttgaag atgtattgcc aatttcaaac ttcacagaca
    37801 caatggagtt ggaatttgtt ggatatgaaa tcaaggaacc aaaatacacg ctagaagaag
    37861 ctcgtatcca cgatgctagc tactcagcac caatttttgt aaccttccgt ttgatcaata
    37921 aagaaacagg cgaaatcaag acccaagaag ttttctttgg tgatttccca atcatgacag
    37981 aaatgggtac tttcatcatc aatggtggtg aacgtattat cgtttctcag ttggtccgct
    38041 caccaggtgt ttactttaac gacaaagtag acaaaaatgg taaggtgggc tatggttcaa
    38101 ctgttatccc taaccgtgga gcttggttgg aacttgaaag cgactcaaaa gatatcacct
    38161 acactcgtat cgaccgtact cgtaagattc catttacaac cttggttcgt gctcttggtt
    38221 tctcaggtga tgatgaaatc tttgatatct ttggtgacag cgaattggtt cgcaacactg
    38281 ttgaaaaaga tatccacaag aatccaatgg actctcgtac agacgaagcc ttgaaagaaa
    38341 tttacgaacg ccttcgtcca ggtgagccta agacggctga aagctcacgt agcttgcttg
    38401 tagctcgctt ctttgaccca cgtcgttatg acttggcagc agttggtcgt tacaaaatca
    38461 ataaaaaact caatgttaaa acacgtttgc tcaaccaaac cattgcagag ccattggtag
    38521 accctgaaac tggagaaatc ttggtagaag ctggtacgat tatgactcgt agcgtgattg
    38581 aaagcattga aagccatttg gatggcgact tgaacaagat tgtctacatc ccaaacgatg
    38641 cagccgttgt gactgagcct gttgttcttc aaaaattcaa ggttattgct ccaactgatc
    38701 cagatcgcgt cgtaacgatc attggtaatg ctaacccaga tgacaaggtt cgtacggtga
    38761 ctcctgcaga tatccttgct gagatgagct acttcctcaa cttggctgaa ggacttggcc
    38821 gtgtagatga tatcgaccac cttggaaatc gtcgtatccg tgcggttggt gaattgcttg
    38881 ccaaccaagt acgtttggga ctttctcgta tggaacgtaa tgtccgtgaa cgtatgtctg
    38941 ttcaggacaa tgaagtcttg acaccacaac aaattatcaa tattcgtcct gtaacagctg
    39001 cagttaaaga attctttggt tcatcacagt tgtcacagtt catggaccaa cacaacccgc
    39061 tttctgagtt gtctcacaaa cgccgtttgt cagccttagg acctggtggt ttgactcgtg
    39121 accgtgccgg atatgaagtg cgtgacgtgc actacactca ctatggtcgt atgtgtccaa
    39181 tcgagacacc tgaaggacct aacatcggtt tgatcaataa cttgtcatct tacggacact
    39241 tgaacaaata tggttttgtt caaacaccat accgtaaggt tgaccgtgaa acaggtgttg
    39301 tcacgaacga aattgtttgg ttgacagctg atgaagaaga tgaatatact gtagctcagg
    39361 ctaactctcg tctgaatgaa gatggaacct ttgctgagaa gattgtcatg ggacgtcacc
    39421 aaggggtcaa ccaagagtat ccagctaata ttgttgacta catggacgtt tcaccaaaac
    39481 aggtagttgc cgttgcgaca gcatgtattc ctttcttgga aaacgatgac tccaaccgtg
    39541 ccctcatggg agccaatatg caacgtcagg ctgtgccatt gattaatcct caggcacctt
    39601 acgttggtac tggtatggaa taccaagcag cccacgattc tggtgcggct gtgattgctc
    39661 agtatgatgg taaagttact tacgcagatg ctgacaaggt agaagttcgt cgtgaagatg
    39721 gttcattgga tgtttaccac atccaaaaat tccgtcgttc aaactcaggt actgcttaca
    39781 accaacgcac tctcgtaaaa gttggtgatg tcgttgaaaa aggcgatttc atcgctgacg
    39841 gaccttctat ggaaaatgga gaaatggcgc ttggacaaaa cccaatcgtt gcctacatga
    39901 cttgggaagg ctacaacttc gaggatgccg ttatcatgag cgaacgcttg gtgaaggacg
    39961 atgtctacac atctgttcac cttgaagaat acgaatcaga aacgcgcgat acaaagcttg
    40021 ggcctgaaga aatcactcgc gaaattccaa acgttggtga agatgccctc aaagaccttg
    40081 acgaaatggg gattatccgt attggtgctg aggttaaaga aggtgatatt cttgtaggga
    40141 aagtaacacc taagggtgag aaagatcttt cagctgaaga acgtctcttg cacgctatct
    40201 ttggagacaa gtctcgtgaa gtgcgtgata cttctcttcg tgtaccacac ggtgccgatg
    40261 gtgtcgttcg tgatgttaag atctttacac gtgtaaatgg agatgagttg caatcaggtg
    40321 ttaacatgtt ggttcgtgtt tacatcgctc aaaaacgtaa gattaaggtc ggagataaaa
    40381 tggccggacg tcacggaaac aaaggggttg tctctcgtat cgttcctgta gaagacatgc
    40441 cttaccttcc agacggaact ccagtcgaca tcatgttgaa cccacttggg gtgccatcac
    40501 gtatgaatat cggtcaggtt atggagcttc accttggtat ggcagctcgt actcttggta
    40561 ttcacattgc gacaccagtc tttgatggag caagttctga agatctttgg tcaactgtta
    40621 aagaagcagg tatggatagc gatgccaaaa cgatccttta cgatggacgt actggtgaac
    40681 catttgataa ccgtgtttct gttggagtca tgtacatgat caaactccac cacatggttg
    40741 acgataaatt gcacgcgcgt tcagtcggac cttactcaac tgttacccaa caaccactcg
    40801 gaggtaaagc tcagtttggt ggacaacgtt tcggtgagat ggaggtttgg gctcttgaag
    40861 cctacggtgc gtcaaatgtc cttcaagaaa tcttgactta caagtctgac gatatcaacg
    40921 gacgtttgaa agcctatgaa gctattacaa aaggcaaacc aattccaaaa ccaggtgttc
    40981 cagaatcctt ccgagttctt gttaaagaat tgcaatctct tggtcttgac atgcgtgtcc
    41041 tagacgaaga tgaccaagaa gtggaacttc gcgacttgga tgaaggaatg gacgaagatg
    41101 tcatccacgt agatgacctt gaaaaagccc gcgaaaaagc agcccaagag gctaaagcag
    41161 cctttgaagc tgaagaagct gagaaagcaa caaaagcgga agcaacagaa gaagctgctg
    41221 aacaagaata agcagttcac ttagaataga aagggaagaa atagtggttg atgtaaatcg
    41281 ttttaaaagt atgcaaatca ccctagcttc tccaagtaaa gtccgttcat ggtcttatgg
    41341 agaagtcaaa aaacctgaaa caatcaatta tcgtaccttg aaaccagaac gtgaaggact
    41401 ctttgatgaa gtgatctttg gtcctacaaa agactgggaa tgtgcttgtg gtaagtacaa
    41461 acgcattcgt tacagaggaa ttgtttgtga ccgctgtggg gttgaagtaa cgcgtacgaa
    41521 agttcgtcgt gagcgtatgg gacatatcga attgaaagct cctgtatctc acatctggta
    41581 cttcaagggg attccaagcc gtatgggctt gacccttgat atgagccctc gtgccctcga
    41641 ggaagttatc tactttgcgg cttatgtggt gattgatcct aaggatacac cacttgagca
    41701 caagtctatc atgacagagc gcgaataccg agagcgcttg cgcgaatatg gttatggttc
    41761 atttgttgcc aagatgggtg cggaagccat ccaagacctt ttgaagcaag tagatcttga
    41821 aaaagaaatt gctgaactca aagaagaatt gaaaacagct actggacaaa aacgtgtcaa
    41881 agccatccgt cgtttggatg ttttggatgc cttttacaag tctggaaaca aacctgaatg
    41941 gatgattctt aacatccttc cggttatccc accagatctt cgtccaatgt tgcagttgga
    42001 tggtggccgt tttgcctcat ctgacttgaa tgacctttac cgccgtgtta tcaatcgtaa
    42061 caaccgtttg gctcgtttgc ttgagttaaa tgcaccaggt atcatcgttc aaaatgagaa
    42121 acgtatgctt caagaagcag ttgacgcttt gattgacaat ggtcgtcgtg gtcgtccaat
    42181 cacaggacca ggtagccgtc cattgaaatc attgagccac atgcttaaag gtaaacaagg
    42241 acgtttccgt caaaacttgc tcggtaaacg tgttgacttc tcaggacgtt cagttatcgc
    42301 cgttggtcca actcttaaga tgtaccaatg tggtgtgcca cgtgaaatgg cgattgaact
    42361 ctttaaacca tttgtcatgc gtgaaatcgt tgcccgtgat atcgtgcaaa acgtcaaagc
    42421 agctaaacgc ttggtggaac gcggagatga gcgtatctgg gatatccttg aagaagtgat
    42481 taaagaacac ccagtgcttt tgaaccgcgc accgaccctt caccgtttgg gtatccaagc
    42541 cttcgagcca gtcttgattg atggtaaggc tcttcgcttg cacccacttg tctgtgaagc
    42601 ctacaatgct gactttgacg gggaccaaat ggccatccac gtaccacttt cagaagaagc
    42661 acaagcagaa gctcgtatcc tcatgctagc tgctgagcac atcttgaacc cgaaagatgg
    42721 gaaaccggta gttactccat ctcaggacat ggttttgggt aactactact tgaccatgga
    42781 agaagctggt cgcgaaggtg aaggaatggt cttcaaagac cgtgacgaag cggttatggc
    42841 ttaccgcaat ggttatgttc acctccactc acgtgttggt atcgcaacag acagcctcaa
    42901 caagccttgg acagaagagc aaagacataa ggtcttgctt acaacagttg gtaaaattct
    42961 cttcaacgat atcatgccag aggggctacc atacttgcaa gaaccaaaca atgccaactt
    43021 gacagaaggt gttccagcta aatacttctt gccacttggt ggagatatca aggaagctat
    43081 cagcaatctt gagctcaacc ctccattcaa gaagaaaaac ctcggaaata tcatcgctga
    43141 aatcttcaaa cgtttccgta cgacagaaac ttctgcccta cttgaccgca tgaagaacct
    43201 cggttaccac cactcaactc ttgcaggatt gacagtgggt attgccgata tcccagtcgt
    43261 tgatgacaag gctgaaatca ttgaagaatc acacaaacgt gtcgaacaaa tcaccaaaca
    43321 attccgtcgt ggtatgatca cagacgacga gcgttacaat gctgttacag ctgaatggcg
    43381 tgctgcccgt gaaaaacttg agaaacgctt gattgctaat caagatccta agaacccaat
    43441 cgttatgatg atggactctg gagcccgtgg taacatctca aacttctcac agcttgccgg
    43501 tatgcgtggt ctgatggctg ctccgaatgg acgtatcatg gaattgccaa tcctttcaaa
    43561 cttccgcgaa ggtttgtcgg tattggaaat gttcttctca actcacggtg ctcgtaaagg
    43621 tatgaccgat acggccctta agacagccga ctcaggttac ttgactcgtc gtttggtcga
    43681 cgttgcccaa gacgttatca tccgtgagga cgactgtgga actgaccgtg gtctcttgat
    43741 ccgttctatc gcagaaggaa aagagatgat cgagtctctc gaagagcgtc tcaacggtcg
    43801 ttacactaag aaaactgtta aacatccaga aactggtgca gtgattattg gtccaaatga
    43861 attgattaca gaagacaagg cgcgtgaaat tgtcaatgct ggtgtggaag aagtgactat
    43921 ccgttctgta tttacatgta acactcgtca cggtgtctgc cgtcactgtt acggtatcaa
    43981 cttggcgact ggtgatgcgg ttgaagttgg tgaagcagtt ggtacaatcg ctgcccaatc
    44041 tatcggggaa cctggtacac agcttacaat gcgtaccttc cacacaggtg gggttgcctc
    44101 aaataccgat atcactcagg gtcttcctcg tgtccaagaa atctttgaag cccgcaatcc
    44161 taaaggggaa gcggttatca cagaggttaa aggacaagtt actgctatcg aagaggatgc
    44221 atcaactcgt accaagaaag tctttgttaa gggtgaaact ggcgaaggtg aatatgtcgt
    44281 tccatttaca gctcgtatgc gtgtcgaagt tgggggccaa gtagcgcgtg gtgctgctct
    44341 gacagaaggt tctatccaac caaaacgtct ccttgcagtt cgtgatgtct tgtcagttga
    44401 aacgtacctt ctcggtgaag tacaaaaagt ttaccgtagc caaggggtag aaatcggtga
    44461 caaacacatc gaggtaatgg ttcgtcaaat gatccgtaaa gtccgtgtca tggatccagg
    44521 tgatacagat cttctcatgg gtaccctcat ggatatcaat gactttacag atgccaacaa
    44581 agatgtcctt atcgcaggtg gagttccagc gacaggtcgc ccagtcctta tgggaattac
    44641 caaagcctca cttgaaacaa acagtttctt gtcagcggct tccttccagg aaacaactcg
    44701 tgtccttact gacgcagcta tccgtggtaa gaaagaccat ctccttggac ttaaagaaaa
    44761 tgttatcatc ggtaagatca tcccagctgg tactggtatg gcccgttacc gtaaccttga
    44821 accacatgct gtcaacgaag aagaatacct taatcctcca gtagaggaag aaggaaatga
    44881 agaaacaaca gaagtagttg tggatactgc cgttgaaact gtggaagaaa cagtagaata
    44941 agagagaatg agagaaccct cgggttctct cattctctct tctgaaaact ttctccattt
    45001 ttccacgaaa tgtggtaaaa taaaagaaag aagctgacaa aggagacggg tatggaacaa
    45061 acattcttta tcatcaaacc agatggtgta aaaagagggc tagtgggtga agtgttaaag
    45121 cgcatcgaac aacgtggatt tacaatcgaa aaattggagt ttcgttcaca ggtttcagaa
    45181 gagttgattg accagcacta tcaggacttg gttggtcaga gtttttaccc accgattcgt
    45241 gaattcatga cttcaggtcc agttcttgtg ggtgtcattt ctggtcccaa agtaatcgaa
    45301 acttggcgga ccatgatggg tgcaactcgt ccagaagaag ctttaccagg cactattcga
    45361 ggtgattttg caaaagctgc tggagaaaat gagattatcc aaaatgttgt acatggttca
    45421 gattcagaag agtcagctaa gcgagaaatt gctctttggt tttaagagtg gattggctca
    45481 atcaattggt taaaagctca tttgaataga aagtatagtc aattagttta agacatgacg
    45541 catgatatca aactttttag tttttgatat ggtgcgtttt ttgattaagt gactattagt
    45601 ttgtctcgct ccgttaggtg cgagacaaaa aaaacacccg ctatgcgggt gcgcgtcgaa
    45661 ggttatacca aaaaaactcc aaacgcgata caataaaggt gttcaatcca attgtaaagc
    45721 gaaaggagaa aaatatggca caaaaggttc ttttatcctg ttcttgtttc aattgactat
    45781 atttctatac tttttcttcc tttctctgtg aaaatcgcaa gtttttccca ccttttctca
    45841 atactgaaac aacatctttc cagaacttca ataatttctt tttgtgttaa atcattattt
    45901 aactaaatct ttggaggcaa tacgttttca aagctacttt tttaccatag gttttgagcc
    45961 ttcaacacag attactaagc caaaaatgct ctaaattttt gaaattctaa caaattccac
    46021 tcaactttat catcatcttt gactttcaca atatcttcct caaaataaca tggaataaag
    46081 atagttttct tttaaaacgg ttgtaggagg aaaatctctc ctacactatt attgattctg
    46141 agagaaaatt gcaaaatatc atctaccaaa ctttcaatta ttaagatttt ttgcagttta
    46201 tctgaattag aatcgcaagg cttctcaata atataattgt cgaatttttt cattctaac
//
LOCUS       DAVMGZ010000004        44283 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000004 DAVMGZ010000000
VERSION     DAVMGZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44283
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.004"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            24..536
                     /locus_tag="VYQ93_000150"
     CDS             24..536
                     /locus_tag="VYQ93_000150"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV3334911.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            718..1662
                     /locus_tag="VYQ93_000151"
     CDS             718..1662
                     /locus_tag="VYQ93_000151"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV3334912.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFLEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            1671..2348
                     /locus_tag="VYQ93_000152"
     CDS             1671..2348
                     /locus_tag="VYQ93_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV3334913.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLIQVSADFRN
                     AMDLWKD"
     gene            2446..3204
                     /locus_tag="VYQ93_000153"
     CDS             2446..3204
                     /locus_tag="VYQ93_000153"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV3334914.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            3242..4747
                     /gene="lytC"
                     /locus_tag="VYQ93_000154"
     CDS             3242..4747
                     /gene="lytC"
                     /locus_tag="VYQ93_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV3334915.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGTRYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKVIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAESDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTGTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPHYSGKKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            5243..5761
                     /locus_tag="VYQ93_000155"
     CDS             5243..5761
                     /locus_tag="VYQ93_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV3334916.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            5891..6397
                     /locus_tag="VYQ93_000156"
     CDS             5891..6397
                     /locus_tag="VYQ93_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV3334917.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYILGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            6397..6567
                     /locus_tag="VYQ93_000157"
     CDS             6397..6567
                     /locus_tag="VYQ93_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3334918.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            6599..7831
                     /gene="clpX"
                     /locus_tag="VYQ93_000158"
     CDS             6599..7831
                     /gene="clpX"
                     /locus_tag="VYQ93_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV3334919.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRMGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            7840..8427
                     /locus_tag="VYQ93_000159"
     CDS             7840..8427
                     /locus_tag="VYQ93_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV3334920.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            8438..8818
                     /locus_tag="VYQ93_000160"
     CDS             8438..8818
                     /locus_tag="VYQ93_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV3334921.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            8870..9760
                     /gene="rapZ"
                     /locus_tag="VYQ93_000161"
     CDS             8870..9760
                     /gene="rapZ"
                     /locus_tag="VYQ93_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV3334922.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            9757..10734
                     /locus_tag="VYQ93_000162"
     CDS             9757..10734
                     /locus_tag="VYQ93_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV3334923.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            10731..11642
                     /gene="whiA"
                     /locus_tag="VYQ93_000163"
     CDS             10731..11642
                     /gene="whiA"
                     /locus_tag="VYQ93_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV3334924.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(11676..12644)
                     /locus_tag="VYQ93_000164"
     CDS             complement(11676..12644)
                     /locus_tag="VYQ93_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV3334925.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELESVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            12780..13637
                     /locus_tag="VYQ93_000165"
     CDS             12780..13637
                     /locus_tag="VYQ93_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV3334926.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            13624..14403
                     /locus_tag="VYQ93_000166"
     CDS             13624..14403
                     /locus_tag="VYQ93_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV3334927.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            14427..15779
                     /locus_tag="VYQ93_000167"
     CDS             14427..15779
                     /locus_tag="VYQ93_000167"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV3334928.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVNYYVDTITDVVRAEIGID
                     "
     gene            15853..16227
                     /locus_tag="VYQ93_000168"
     CDS             15853..16227
                     /locus_tag="VYQ93_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV3334929.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            16229..17077
                     /locus_tag="VYQ93_000169"
     CDS             16229..17077
                     /locus_tag="VYQ93_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV3334930.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            17343..18110
                     /locus_tag="VYQ93_000170"
     CDS             17343..18110
                     /locus_tag="VYQ93_000170"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV3334931.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWIDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            18122..19306
                     /locus_tag="VYQ93_000171"
     CDS             18122..19306
                     /locus_tag="VYQ93_000171"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV3334932.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            19303..21174
                     /locus_tag="VYQ93_000172"
     CDS             19303..21174
                     /locus_tag="VYQ93_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV3334933.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKSDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLNKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMSRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(21317..22501)
                     /gene="mntE"
                     /locus_tag="VYQ93_000173"
     CDS             complement(21317..22501)
                     /gene="mntE"
                     /locus_tag="VYQ93_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV3334934.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPILEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            22805..25501
                     /locus_tag="VYQ93_000174"
     CDS             22805..25501
                     /locus_tag="VYQ93_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV3334935.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAMDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFASDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            25514..26305
                     /gene="yghU"
                     /locus_tag="VYQ93_000175"
                     /pseudo
     CDS             25514..26305
                     /gene="yghU"
                     /locus_tag="VYQ93_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
     gene            26421..26831
                     /locus_tag="VYQ93_000176"
     CDS             26421..26831
                     /locus_tag="VYQ93_000176"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV3334936.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            26843..28438
                     /locus_tag="VYQ93_000177"
     CDS             26843..28438
                     /locus_tag="VYQ93_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3334937.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADFVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            28605..28769
                     /locus_tag="VYQ93_000178"
     CDS             28605..28769
                     /locus_tag="VYQ93_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV3334938.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            28848..29576
                     /gene="yaaA"
                     /locus_tag="VYQ93_000179"
     CDS             28848..29576
                     /gene="yaaA"
                     /locus_tag="VYQ93_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV3334939.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(29854..30402)
                     /locus_tag="VYQ93_000180"
     CDS             complement(29854..30402)
                     /locus_tag="VYQ93_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV3334940.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            30723..31211
                     /locus_tag="VYQ93_000181"
     CDS             30723..31211
                     /locus_tag="VYQ93_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV3334941.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGISQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            31208..32395
                     /locus_tag="VYQ93_000182"
     CDS             31208..32395
                     /locus_tag="VYQ93_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV3334942.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     CTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            32388..32612
                     /locus_tag="VYQ93_000183"
     CDS             32388..32612
                     /locus_tag="VYQ93_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV3334943.1"
                     /translation="MIDHFEIKVKDLQISGGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            32769..34115
                     /gene="asnS"
                     /locus_tag="VYQ93_000184"
     CDS             32769..34115
                     /gene="asnS"
                     /locus_tag="VYQ93_000184"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV3334944.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            34268..34558
                     /locus_tag="VYQ93_000185"
     CDS             34268..34558
                     /locus_tag="VYQ93_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV3334945.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            34570..35040
                     /gene="ssbA"
                     /locus_tag="VYQ93_000186"
     CDS             34570..35040
                     /gene="ssbA"
                     /locus_tag="VYQ93_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV3334946.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            35072..35311
                     /locus_tag="VYQ93_000187"
     CDS             35072..35311
                     /locus_tag="VYQ93_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV3334947.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            35606..37006
                     /locus_tag="VYQ93_000188"
     CDS             35606..37006
                     /locus_tag="VYQ93_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV3334948.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            37008..37367
                     /locus_tag="VYQ93_000189"
     CDS             37008..37367
                     /locus_tag="VYQ93_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV3334949.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            37421..38170
                     /locus_tag="VYQ93_000190"
     CDS             37421..38170
                     /locus_tag="VYQ93_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV3334950.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            38160..38459
                     /locus_tag="VYQ93_000191"
     CDS             38160..38459
                     /locus_tag="VYQ93_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV3334951.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            38530..39465
                     /locus_tag="VYQ93_000192"
     CDS             38530..39465
                     /locus_tag="VYQ93_000192"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV3334952.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            39551..40063
                     /locus_tag="VYQ93_000193"
     CDS             39551..40063
                     /locus_tag="VYQ93_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV3334953.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            40100..40696
                     /locus_tag="VYQ93_000194"
                     /pseudo
     CDS             40100..40696
                     /locus_tag="VYQ93_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            40797..40991
                     /locus_tag="VYQ93_000195"
     CDS             40797..40991
                     /locus_tag="VYQ93_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV3334954.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVSKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(41037..42482)
                     /locus_tag="VYQ93_000196"
     CDS             complement(41037..42482)
                     /locus_tag="VYQ93_000196"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV3334955.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYIAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISLDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FRFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            42588..44210
                     /locus_tag="VYQ93_000197"
     CDS             42588..44210
                     /locus_tag="VYQ93_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV3334956.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
BASE COUNT        12886 a         8382 c        10213 g        12802 t
ORIGIN      
        1 aaaactttga aagaaagaaa aagatgaatt taaaagatta cattgcaaca attgaaaatt
       61 atccaaagga aggcattacc ttccgtgata ttagtccttt gatggctgat ggaaatgctt
      121 atagctacgc tgttcgtgaa atcgttcagt atgctactga caagaaagtc gacatgatcg
      181 tgggacctga agctcgtgga tttatcgtgg gttgtccagt tgcctttgag ttgggaattg
      241 gttttgcgcc tgttcgtaag ccaggtaaat tgccacgcga agttatttct gctgactatg
      301 aaaaagagta cggtgtcgat accttgacta tgcacgcgga tgccattaag ccaggtcaac
      361 gtgttcttat tgtagatgac cttttggcga caggtggaac tgttaaggca actatcgaga
      421 tgattgaaaa acttggtggt gttatggcag gttgtgcctt ccttgttgaa ttggatgaat
      481 tgaacggccg tgaaaaaatc ggtgactacg actacaaagt tcttatgcat tattaatgaa
      541 aacagtccct agggctgttt tctctacact aggatataaa aatagactat aactagttag
      601 agaaaaacta taattgaaaa ctatatcttc ttgcagtata ataaaaggac taagtgtttg
      661 agatttgtct tcaaacatat gcaattattc ctgaaagagt acagttagga gagggttatg
      721 ccgattcgaa ttgataaaaa attgccagct gttgagattt tacggacaga gaatatcttt
      781 gtcatggatg atcaacgtgc tgcccaccaa gatatccgtc ctttgaagat tttaatttta
      841 aatctcatgc cacagaaaat ggtcacagag acccagttgt tgcgccactt ggctaataca
      901 cccctacaac tggatattga ttttctctat atggagagcc accgttctaa aacaactcgt
      961 tcagagcaca tggagacctt ctataaaact tttcttgaag tcaaggatga gtattttgat
     1021 gggatgatca tcacgggtgc tccagttgag catttaccat ttgaggaagt ggactattgg
     1081 gaggaattta gacagatgct tgagtggtct aagactcatg tctattcgac ccttcatatc
     1141 tgttgggggg ctcaggctgg gctttatctg cgctatggtg tagaaaaata ccagatggac
     1201 agtaagctat caggtattta tcctcaggac accctaaaag agggtcacct tctatttaga
     1261 ggctttgatg atagctatgt atctcctcat tcacggcaca cggagatttc taaggaagag
     1321 gtcttaaaca agaccaatct cgagatttta tcagaaggac ctcaggttgg ggtttctatt
     1381 ttggccagtc gtgatttacg agaaatttat agttttggtc atttggagta tgaccgtgat
     1441 accttggcaa aagagtattt tcgagatcgt gatgcaggtt tcgacccaca tattccagaa
     1501 aattacttta aagatgatga tgttaatcag gtaccttgtc tttgttggtc ttcatctgca
     1561 gccctctttt tcagtaattg ggtaaaccat gcggtctatc aggagacgcc ttttgactgg
     1621 agaaagatag aagatgatgc atctgcatat gggtatttat aagaggaatt atgacatatt
     1681 tagacgcttt taaatcaggt accttggttt taccgagtgc cctgctcttg cattttaagg
     1741 aactctttcc ttctagcgac gattttctgg tttggcaatt tttctatttg caaaatacga
     1801 caggcttaga agaaatgtcg ccaagccaga ttgctgaaag gattggcaag gaaatttcgg
     1861 atgtcaacca gtccatttct aatctgacgg aaaggggact gctccagtat cgtactatcg
     1921 aattaaatgg cgaaattgaa ttgctctttg atgctagttt ggccttggaa cgtttggatg
     1981 acctgtttgg agcagttcat tcaagttcag accagctaac acctcaaaac cagctcaagg
     2041 atttggtgga aaccttccag caggagttgg gacgattgtt gacgcctttt gagattgagg
     2101 atttgaccaa gacactaaag gaagatggaa ccagtgctga cttgattaag gaggctcttc
     2161 gtgaagctgt tttgaatgga aaaccaaact ggaagtacat tcaggcgatt ttgagaaact
     2221 ggcgccatga aggaatcaag agtgtggctc aaattgaggc caagagagca gaaagagaag
     2281 caagcaatcc tcagttgata caggtatctg cagatttcag aaatgccatg gatctctgga
     2341 aggattaatc catgcaagta ggcttgaaat ccgagtaaga tttgcaagct gtgtataatt
     2401 gtgatagaat aaatagaaaa taaattgaaa aaagaggtat gtgaaatgtc acgtaaacca
     2461 tttatcgctg gtaactggaa aatgaacaaa aatccagaag aagctaaagc attcgttgaa
     2521 gcagttgcat caaaacttcc ttcatcagat cttgttgaag caggtatcgc tgctccagct
     2581 cttgatttga caactgttct tgctgttgca aaaggctcaa accttaaagt tgctgctcaa
     2641 aactgctact ttgaaaatgc aggtgctttc actggtgaaa ctagcccaca agttttgaaa
     2701 gaaatcggta ctgactacgt tgttatcggt cactcagaac gccgtgacta cttccatgaa
     2761 actgacgaag atatcaacaa aaaagcaaaa gcaatctttg cgaacggtat gcttccaatc
     2821 atctgttgtg gtgaatcact tgaaacttac gaagctggta aagctgctga attcgtaggt
     2881 gctcaagtat ctgctgcatt ggctggattg actgctgaac aagttgctgc ctcagttatc
     2941 gcttatgagc caatctgggc tatcggtact ggtaaatcag cttcacaaga cgatgcacaa
     3001 aaaatgtgta aagttgttcg tgacgttgta gctgctgact ttggtcaaga agtcgcagac
     3061 aaagttcgtg ttcaatacgg tggttctgtt aaacctgaaa atgttgcttc atacatggct
     3121 tgcccagacg ttgacggtgc ccttgtaggt ggtgcgtcac ttgaagctga aagcttctta
     3181 gctttgcttg actttgtaaa ataatcagta agtagcaaaa gctaggtgga acagcattca
     3241 gatgtctgtt acatttttta taggagagca aagattgaaa acaaaaattg gattagcaag
     3301 tatctgttta ctaggcttgg caactagtca tgtcgctgca aatgaaactg aagtagcaaa
     3361 aacttcgcag gatacaacga cagcttcaag tagttcagag caaaatcagt cttctaataa
     3421 aacgcaaacg agcgcagaag tacagactaa tgctgctgcc cactgggatg gggattatta
     3481 tgtaaaggat gatggttcta aagctcaaag tgaatggatt tttgacaact actataaggc
     3541 ttggttttat attaattcag atggtcgtta ctcgcagaat gaatggcatg gaaattacta
     3601 cctgaaatca ggtggatata tggcccaaaa cgagtggatc tatgacagta attacaagag
     3661 ttggttttat ctcaagtcag atggggctta tgctcatcaa gaatggcaat tgattggaaa
     3721 taagtggtac tacttcaaga agtggggtta catggctaaa agccaatggc aaggaagtta
     3781 tttcttgaat ggtcaaggag ctatgatgca aaatgaatgg ctctatgatc cagcctattc
     3841 tgcttatttt tatctaaaat ccgatggaac ttatgctaac caagagtggc aaaaagtggg
     3901 cggcaaatgg tactatttca agaagtgggg ctatatggct cggaatgagt ggcaaggcaa
     3961 ctactatttg actggaagtg gtgccatggc gactgacgaa gtgattatgg atggtactcg
     4021 ctatatcttt gcggcctctg gtgagctcaa agaaaagaaa gatttgaatg tcggctgggt
     4081 tcacagagat ggtaagcgct atttctttaa taatagagaa gaacaagtgg gaaccgaaca
     4141 tgctaagaaa gtcattgata ttagtgagca caatggtcgt atcaatgatt ggaaaaaggt
     4201 tattgatgag aacgaagtgg atggtgtcat tgttcgtcta ggttatagcg gtaaagaaga
     4261 caaggaattg gcgcataaca ttaaggagtt aaaccgtctg ggaattcctt atggtgtcta
     4321 tctctatacc tatgctgaaa atgagaccga tgctgagagt gacgctaaac agaccattga
     4381 acttataaag aaatacaata tgaacctgtc ttaccctatc tattatgatg ttgagaattg
     4441 ggaatatgta aataagagta agagagctcc aagtgataca ggcacttggg ttaaaatcat
     4501 caacaagtac atggacacga tgaagcaggc gggttatcaa aatgtgtatg tctatagcta
     4561 tcgtagttta ttacagacgc gtttaaaaca cccagatatt ttaaaacatg taaactgggt
     4621 agcggcctat acgaatgctt tagaatggga aaaccctcat tattcaggaa aaaaaggttg
     4681 gcaatatacc tcttctgaat acatgaaagg aatccaaggg cgcgtagatg tcagcgtttg
     4741 gtattaagcg atgatttgaa agagggatgt gatagtagca ccctcttttt ctttgtttta
     4801 tgatagttca tcctcgagta aattcaagat cttgctcgga aatgaagctt atatagtaga
     4861 ttgaatatag acaaatacct tgtgattggt aaaacatttt agaaattcat ttacctttcc
     4921 taatcgactt ggtttcatct tatttcaatc tattatagta ttggggaatt tcttcaaacc
     4981 acatcagctt ggtcagttct acctgcgacc tcaaaacttg tgctttggtc aagctgggtt
     5041 tagtttccta gtttgctgat ggatttccat tgactataag catccaaccc tctttttgtc
     5101 ttctaaagaa ttcttaaatt atcagtctat tgcaactttt ctcatataag ttctttgtct
     5161 tgctattggt tttccttagt agtatactaa ggtagtaatc attaagaagt ggttacaaaa
     5221 aataatgaat gaggtaaaga aaatggtaga attgaaaaaa gaagcagtaa aagacgtaac
     5281 atcattgaca aaagcagcgc cagtagcatt ggcaaaaaca aaggaagtct tgaaccaagc
     5341 tgttgctgat ttgtatgtag ctcacgttgc tttgcaccaa gtgcactggt atatgcatgg
     5401 tcgtggtttc cttgtatggc atccaaaaat ggatgagtac atggaagctc ttgacggtca
     5461 attggatgaa atcagcgaac gcttgattac actcggtgga agcccattct ctacattgac
     5521 agagttcctt caaaatagtg aaatcgaaga agaagctggt gaataccgta atgttgaaga
     5581 aagcttggaa cgtgttcttg ttatctaccg ttacttgtca gaacttttcc aaaaaggttt
     5641 ggatgtcact gatgaagaag gtgacgatgt aacaaacggt atctttgcag gcgctaaaac
     5701 tgaaacagat aaaacaattt ggatgcttgc agccgaactt ggacaagcac ctggtttgta
     5761 agaaataagg aaacatagat aaatctgtag gaaatttctt acagattttt tctattttgt
     5821 aagctattcc aaaccagtct ttttttgagt tttttgataa aatagtacta tcagtgaaaa
     5881 ggatggaagc atgactaaga aaatcgtagc tatttgggcc caggatgaag agggtgtgat
     5941 tggtaaggaa aatcgtctgc cttggcattt gccagcagaa ttgcagcact ttaaagaaac
     6001 aactctgaat catgctatct tgatggggcg tgtgaccttt gatgggatgg ggcgtcgctt
     6061 gcttccaaaa cgggaaacct tgattttgac gcgtaatccg gaagaaaaga tagatggggt
     6121 tgctactttt caggacgtcc agtctgttct tgactggtat caggctcaag aaaagaatct
     6181 ctacattctc ggtgggaagc aaatttttca ggcttttgaa gcttaccttg atgaagtgat
     6241 tgtcactcac attcatgctc gggtggaagg agatacctat ttccctgaag aacttgactt
     6301 gtctcttttt gagacagttt caagcaaatt ttacgccaaa gatgagaaga atccttatga
     6361 ttttaccatc caataccgca agagaaagga agtctaatgg aacggagttt atttggtcta
     6421 ttcacggctt ttctgtgttt catctgcttt ctggcaggag cacaagcttt tcgtaaaaag
     6481 cgttacgggc tttctatcct cctttggtta aatgccttta ccaatctggt caatagtatt
     6541 catgcttttt acatgacctt attttagata gaatgacagt atagaatgga aggaaatcat
     6601 gtctacaaat agaaaaaatg atatgatggt ttattgctca ttttgtggca aaaaccaaga
     6661 agaagtacaa aaaataattg ctggcaacaa tgcttttatt tgtaatgaat gcgtggagtt
     6721 agctcaggaa atcattcgag aagaattggt tgaggaagtc ttggcagact tgtctgaggt
     6781 gccaaaacca attgaactcc tccatatctt gaaccactat gtaattggtc aagatcgtgc
     6841 caagcgtgcc ttggcagtgg cggtttataa ccactacaaa cgcatcaatt tccacgatac
     6901 acgcgaagag tcagaagatg tggatttgca gaagtcaaac attttgatga ttggcccaac
     6961 tggttcaggg aaaactttcc ttgcccagac cttggctaag agcttgaatg taccttttgc
     7021 tattgcggat gcgacagctc tgacggaggc tggttatgtg ggtgaggatg tggaaaatat
     7081 cctcctcaaa ctcttgcagg ttgctgactt taacatcgaa cgtgcagagc gtggcattat
     7141 ctatgtggat gaaattgaca agattgccaa gaagagtgag aatgtgtcta tcacacgtga
     7201 tgtttctggt gaaggggtgc aacaagccct tctcaagatt atcgagggaa ctgttgctag
     7261 cgtgccgcct caaggtggac gcaaacatcc acaacaagag atgattcaag tggatacaaa
     7321 aaatatcctc ttcatcgtgg gtggtgcttt tgatggtatt gaagaaattg tcaaacaacg
     7381 tatgggtgaa aaagtcatcg gatttggtca aaacaataag gcgattgacg aaaacagctc
     7441 atacatgcaa gaaatcatcg ctgaagacat tcaaaaattt ggtattatcc ctgagttgat
     7501 tggacgcttg cctgtttttg cggctcttga gcaattgact gttgatgact tggttcgcat
     7561 cttgaaagag ccaagaaatg ccttggtgaa acaataccaa accttgcttt cttatgatga
     7621 tgttgagttg gaatttgacg acgaagccct tcaagagatt gctaataaag caatcgaacg
     7681 gaagacaggg gcgcgtggac ttcgctccat tatcgaagaa accatgctag atgttatgtt
     7741 tgaggtgccg agtcaggaaa atgtgaaatt ggttcgcatc actaaagaaa ctgtcgatgg
     7801 aacggataaa ccgatcctag aaacagccta gaggtgacta tggaacttaa tacacacaat
     7861 gctgaaatct tgctcagtgc agctaataag tcccattatc cgcaggatga actgccagag
     7921 attgccctag cagggcgttc aaatgttggt aaatccagct ttatcaacac tatgttgaac
     7981 cgtaagaatc tcgcccgtac atcaggaaaa cctggtaaaa cccagctcct gaactttttt
     8041 aacattgatg acaagatgcg ctttgtggat gtgcctggtt atggctatgc ccgtgtttct
     8101 aaaaaggaac gtgaaaagtg ggggtgcatg attgaggagt acttaacgac tcgggaaaat
     8161 ctccgtgcgg ttgtcagtct agttgacctt cgtcatgacc cgtcagcaga tgatgtgcag
     8221 atgtacgaat ttctcaagta ttatgagatt ccagtcatca ttgtggcgac caaggcggac
     8281 aagattcctc gtggtaaatg gaacaagcat gaatcagcaa tcaaaaagaa attaaacttt
     8341 gacccaagtg acgatttcat cctcttttca tctgtcagca aggcagggat ggatgaggct
     8401 tgggatgcaa tcttagaaaa attgtgagga aaagaaaatg gcaaaaacaa ttcatacaga
     8461 taaggcccca aaggctatcg gaccctatgt tcaaggaaaa atcgttggca accttttgtt
     8521 tgctagcggt caagttcccc tatcccctga aactggggaa attgtaggag agaatatcca
     8581 agaacagaca gagcaagtct tgaaaaacat cggtgctatt ttggcagaag caggaacaga
     8641 ctttgaccat gttgtcaaaa caacttgctt cttgagcgat atgaacgact ttgttccttt
     8701 taatgaggtt taccaaacgg ccttcaaaga ggaattccca gctcgttcag ctgtggaggt
     8761 agctcgtctt cctcgtgatg taaaagtcga aattgaagtc atcgcagaga ttggataagc
     8821 tagttgaagt ttggtgttgc caaacttctt ttgatataag gagaaaaaga tgacaaagaa
     8881 acaacttcac ttggtgattg tgacagggat gagtggcgca gggaaaactg tagccattca
     8941 gtccttcgag gatctaggtt atttcaccat tgataatatg ccgccagctc tcttgcctaa
     9001 gtttttgcag ctggttgaaa ttaaggaaga caatcctaag ttggccttgg tagtggatat
     9061 gcgtagccgt tctttctttt cagagattca agctgttttg gatgagttgg aaaatcaaga
     9121 tggtttggat ttcaaaatcc tctttttgga tgcggctgat aaggaattgg tcgctcgtta
     9181 caaggaaacc agacggagtc acccactagc agcagacggt cgtattttag atggaatcaa
     9241 gttggaacgt gaactcttgg cacctttgaa aaatatgagc caaaatgtgg tggatacgac
     9301 tgaactcact ccacgtgagc tgcgcaaaac ccttgcagag cagttttcag accaagaaca
     9361 agcccagtct ttccgtatcg aagtcatgtc tttcggattt aagtatggaa tcccgattga
     9421 tgcggacttg gtctttgatg tccgtttctt gccaaatccc tattatttac cagaactgag
     9481 aaaccaaacg ggtgtggatg aacctgttta tgattatgtc atgaaccatc ctgagtcaga
     9541 agacttttat caacatttat tggccttgat tgagccgatt ctgccaagtt accaaaagga
     9601 aggtaagtcc gttttgacca ttgccatggg atgtacgggt ggacaacacc gtagtgtggc
     9661 atttgctaaa cgcttggtgc aggacttatc caagaattgg tctgttaatg aagggcatcg
     9721 cgacaaagac cgcagaaagg aaacggtaaa ccgttcatga gaaaaccaaa gataacggtg
     9781 attggtggag ggactggaat tcccgtcatt ctaaaaagtc tgcgggaaaa agatgtggaa
     9841 atcgcagcta tcgtgacggt ggcagatgat ggtggttctt caggtgaact ccgaaaaaat
     9901 atgcaacagt tgacaccgcc aggtgatctt cgtaatgtcc ttgtggccat gtcggatatg
     9961 cctaagtttt atgagaaggt ctttcagtat cggttctctg aggatgccgg agcctttgct
    10021 ggccatccat tgggaaatct catcattgct ggcttgtcag aaatgcaggg ttcaacctat
    10081 aatgccatgc agttattgag caaatttttc catacaacag ggaaaattta tccttccagt
    10141 gaccatcctt tgacccttca tgcagtcttt caggatggga cagaagtggc tggagagagt
    10201 catattgtag accatcgagg cataattgac aatgtctatg tgaccaatgc cctaaacgat
    10261 gatacgcctc tggccagccg tcgagtagtg cagaccatcc ttgaaagtga catgattgtc
    10321 ctagggccag gttccctctt tacctctatt ttgcccaata tcgtgattaa ggaaattggg
    10381 cgggctcttt tggaaaccaa ggcagaaatt gcctatgtct gcaatatcat gacccaacgt
    10441 ggggagacgg aacactttac agatagcgac cacgtggaag tcttgcatcg tcaccttggt
    10501 cgccctttta tcgacactgt cttggtgaat attgaaaaag tgcctcagga atacatgaat
    10561 tccaaccgtt ttgatgaata cttggtgcaa gtggaacacg attttgtagg tctttgtaag
    10621 caagtttcgc gcgtgatttc atctaacttc cttcgtctgg aaaatggcgg tgccttccac
    10681 gatggagatt tgattgtgga cgagttgatg cgcattatac aggtgaaaaa atgagtttca
    10741 cagtagcagt aaaagaagaa atcctgggcc aacaccatct gagctggcat gaattatctg
    10801 ccattatcaa aatgtctggt agcatcggtc tctcgacttc gggcttgact ttgtcagttg
    10861 tgacagaaaa tgccaaactg gcccgtcacc tctatgagtc ctttctccat ttctatgaaa
    10921 tcaaatctga aattcgtcac caccaacgga gcaatcttcg caagaatcgc gtctataccg
    10981 tttttacaga tgaaaaggtg caggatttgt taagtgattt gcacttggca gactctttct
    11041 ttggcctgga aacaggtatt gatgaggcga ttttatcgga tgaggaagca ggtcgtgcct
    11101 atctctgtgg cgctttcttg gcaaatggga gcattcgtga ccctgagtca ggcaagtacc
    11161 agttggaaat cagttctgtt tatctggacc acgcgcaagg aattgcctcc cttctccaac
    11221 agtttttact ggatgccaag gtgcttgagc gcaagaaggg ggctgtgacc tatctccagc
    11281 gagcagaaga cattatggac ttcttgatag taatcggagc catgcaggca cgtgatgatt
    11341 ttgagcgggt taagattttg cgagaaaccc gtaacgacct caatcgggct aataatgccg
    11401 agacagctaa tatcgctcgg acagtttctg ccagcatgaa gaccattaac aatatcagta
    11461 aaatcaaaga tatcatgggc ttagaaaatt tgccagtaga tttgcaggaa gtggcgcaac
    11521 tgagaattca gcacccagac tactctatcc agcagttagc agatagtctc agcacccctc
    11581 tgaccaaaag tggtgtcaat cacagactca gaaaaatcaa taagatagcg gatgaattat
    11641 aaaaccacga atcctatgtg actcgtggtt cttttttata aactagtaga gtgttttggt
    11701 tgtacttttt gttcagggtc aatgtagttg atagcgttgt tgacagcagt tggagcttct
    11761 ccgaggcctg tcgcaatcag atcaattttt ccgtcatagt agcagcagtc accgatagca
    11821 tagatacctg cttggctgga ttcctgtttg ctgttgacga taatcttgtg acggttgagg
    11881 tcgagccccc agtttttaag gttaccgaca gaagatttga aaccatagtt gacaaagagg
    11941 tggtctaggt caatggtttc agtttcatca gatttgactt ttgtgatttc aagtttatca
    12001 agtgtttttc catttccaag gagttggcta ggggcgaatg gtgtcttgat ggttacagat
    12061 gattcttgca aggcttgaac actgtgttcc aaggcacgga aattatctct gcggtgaaca
    12121 agggtagttg gtgcgatttt ttcaaaagcc aaagcccaat ccacagccga gtctccccca
    12181 ccaagaatcg tcactttctt accagcgtat tgctgaatgt tagaaacgtg gtagtggata
    12241 ttttcatagc cctcaacact ttcaagttcc agcggacgtg gtttgaaggc accgccaccc
    12301 atagcgatga taactgtttt agtcaggtga cttcctttag aagttgtgat ggcaaattct
    12361 tcttcttgtt tgtcaatctc aagaaccgtt tcattgagat gaataggggt atcaaatcca
    12421 tttagctgtt caatcaagcg gttagtcaac tcttctccag tcaggtttgg gaagcctggt
    12481 acgtctagga tttccttttc agggtagaga atagcaggtt gtccacctag ctggggaaga
    12541 gagtcgatga tttgaacctt ggcttggcgt aggtgggcat aaaaggctgc aaaaagcccg
    12601 acaggaccac cacccacaat ggtaatatca tagagttgag acatagtttc tcctttgttt
    12661 tttctagtca gtttatttta tcatattttt gttcaatgta aaatggacta ggatagggaa
    12721 aaaaatacca gaaaatggta taataggacg aaagtgtttt gatagggagg atatcaggga
    12781 tgaattttca acaattatcc aatctgcaat attggaccag tttgtttgca agtccatgga
    12841 cgatagctat caatctgatt gatattttga ttgttgctta tattttatac cattttacaa
    12901 aagctattgc aggaaccaag attatgattt tggtacgtgg agttttggtg tttattttag
    12961 ctcaaatcct tgcaaatatg attggtttga ctacgatttc ttggttaatc aatcaaatta
    13021 ttacttatgg ggttattgcg gcggttgtta tcttctctcc agagattcgg actggtttgg
    13081 aacgtttggg aagagcgaca gatttctttt ccaatgcccc tattagtgct gaggaacaga
    13141 tgattcgtgc ctttgttaag tctgttgaat acatgagtcc tcgtaaaatc ggggccttgg
    13201 ttgctattca gcgtgtacgt accttgcagg agtatatttc gacaggaatt cccttggatg
    13261 ctaagatttc tgcagaactt ctcattaaca tttttattcc caacactccc ctacatgatg
    13321 gtgcggtgat tatcaaagaa gaacgtatcg ctgtgacgtc tgcctatctg cccttgacaa
    13381 aaaacacagg gatttccaag gaatttggga ccagacaccg ggcggctatc ggtttatcag
    13441 aagtctcaga tgccttgact tttgtcgtat cagaggaaac gggaggaatt tcgataacct
    13501 ataatggaag gtttaagcac aacctaacac ttgatgaatt tgaaacagaa ttacgtgaaa
    13561 tcttacttcc aaaagaggaa gtgggtctta gttttaaaga acgattgcta ggaggatgga
    13621 aacatgaaaa aaaatagttt atatatcata tcctcactct tttttgcttg tgtcttattt
    13681 gtctatgcta cggcgacgaa ttttcaaaac agtaccagtg ctaggcaggt aaaaacggaa
    13741 acctatacta atacagtaac aaatgtccct attgacatac gctataatag tgataagtat
    13801 tttattagcg gttttgcttc agaagtatca gtggtcttga ctggtgcaaa tcgcctatcg
    13861 ctagctagtg aaatgcaaga aagtacacgt aaattcaagg ttactgctga cctaacagat
    13921 gccggtgttg gaacgattga agttcctttg agcattgaag atttacccaa tgggctgacc
    13981 gctgtggcga ctccgcaaaa aattacagtc aagattggta agaaggctca gaaggataag
    14041 gtaaagattg taccagagat tgaccctagt caaattgata gtcgggtaca aattgaaaat
    14101 gtcatggtgt cagataaaga agtgtcgatt acgagtgacc aagagacatt ggatagaatt
    14161 gataagatta tcgctgtttt gccaactagc gaacgtataa caggtaatta cagtggttca
    14221 gtacctttgc aggcaatcga ccgcaatggt gttgtcttac cggcagttat cactccgttt
    14281 gatacaataa tgaaggtgac tacaaaacca gtagcaccaa gttcaagcac atcaaattca
    14341 agtacaagca gttcatcgga gacatcttcg tcaacgaaag caactagttc aaaaacgaat
    14401 taaaaataga aaaggatttt ataacaatgg gtaaatattt tgggactgat ggagtccgtg
    14461 gagaagctaa cctagaacta acaccagaat tagcctttaa actaggacgt tttggaggct
    14521 atgttcttag tcaacatgaa acggaagcgc cgaaagtctt tgtaggacgt gacacacgta
    14581 tttcagggga aatgttggaa tcggccttgg tggcaggtct cctttcagta gggattcacg
    14641 tatacaaact tggtgtcctt gcaacaccag cagtagctta cttggttgaa actgaaggag
    14701 caagtgccgg tgtcatgatt tctgctagcc acaacccagc ccttgataac ggaatcaagt
    14761 tctttggcgg tgatggcttc aaactagatg atgaaaaaga agcagaaatt gaagccttgc
    14821 tagatgctga ggaagacact cttcctcgtc caagtgcaga aggcttagga attttggtag
    14881 attatccaga aggcttgcgt aagtatgaag gataccttgt ttcaactgga actcctcttg
    14941 atggaatgaa ggttgccttg gatacagcta atggagcagc ttctaccagt gcccgtcaaa
    15001 tctttgcaga ccttggtgcc caattgacgg ttatcgggga aacaccagac ggtcttaaca
    15061 tcaaccttaa tgttggttca acacatccag aagcccttca agaagtggtc aaagaaagtg
    15121 ggtcagctat tggtttggcc tttgatggag acagtgaccg cttgattgct gttgatgaga
    15181 atggtgacat cgttgatggt gacaagatta tgtacatcat cggaaaatac ctttctgaaa
    15241 aaggacaatt ggctcaaaat acaattgtga caactgttat gtctaacctt ggtttccaca
    15301 aggccttgaa tcgcgaaggt attaacaagg cagttactgc agttggtgac cgctacgttg
    15361 ttgaagaaat gagaaaatca ggctacaacc ttggtggtga acagtctggt cacgttatct
    15421 tgatggatta caataccaca ggtgatggtc aattatcagc agttcaattg actaaaatca
    15481 tgaaggaaac tggtaagagc ttatcagagt tggcggcaga agtaacgatt tatccacaaa
    15541 aattagttaa tatccgagtg gaaaacgtca tgaaggaaaa ggccatggaa gtgccagcta
    15601 tcaaggccat catcgagaag atggaagaag aaatggcggg gaacggccgt atccttgttc
    15661 gtccaagtgg aacagaaccc ctcttgcgtg ttatggcaga agcgcctaca acagaagaag
    15721 taaactacta tgttgatacc atcacagatg tagttcgtgc tgaaattggg attgactaaa
    15781 tcctagaaaa ctagatgaaa ataaaaaatt atggtacaat agcttataat attgtagccg
    15841 atggagaaag acatgaatct taaacgtgaa caagaatttg ttagtcagta tcattttgat
    15901 gcgcgtaatt ttgaatggga aaatgaaaat ggagctcctg aaaccaaggt ggatgtgaat
    15961 ttccaattgc tccaacatga ccaagaaaat caagtgactt ccttgattgt catcttgagc
    16021 tttatgattg tatttgataa gtttgtcatt agtggaacaa tctcacaagt aaaccatatc
    16081 gatggccgta ttgtcaatga accaagtgaa ttgaaccaag aagaggtgga aaccttggct
    16141 cgtccatgtt tgaacatgct caatcgtttg acttacgaag tgactgaaat tgcattagac
    16201 ttaccaggaa ttaatttgga gttctaatat gaaattagct gttttcacag attcctctgc
    16261 ttatctcagt gcagagatct tgcaaagaga agacttgttt gtcttggata ttcctgtcaa
    16321 tattgatggt gaggaatatg tcgaaggcat caatctgtct gctgaggaat tttaccaaaa
    16381 aatggctcag gcttctgaat tgcctaagac cagtcaacca agtattgcca agttagatga
    16441 aatcttaact tcgctcaaag aacaaggcta tacacatgcc ttggggcttt tcctatcttc
    16501 tggaatttca ggtttttacc aaaatatcca gtatatggtc gatgactatg agggcttaac
    16561 cattgctttc ccagacactt tgattacaag tgctccccta ggcatcatgg ttgaaagcgt
    16621 ctttaattgg cgtgatcagg gcgatgattt tgccagcatt caggataagc tagccattca
    16681 aatcagccgt acgtcagcct ttatcatggt agatgacttg gatcatttgg taaaaggtgg
    16741 acgcctttca aatggggctg ccattttggg caatttgctt agcattaagc caatccttta
    16801 ttttaacgat caaggtgtga ttgaagttta cgaaaaagtt cgtactgaaa agaaggccac
    16861 caaacgctta attgaaatta tcaaggaaac aacggcttca ggtcaatacc gggtcattgt
    16921 cattcatggg aatgctcctg aaaaggctga agaattgcgt cagcatttgc ttgattttgg
    16981 cttgggttcg gatgtttcac ttgctacatt tggtagtgtc attggaacgc acctaggagc
    17041 aggaagtatt gctctaggtt atattccagt gatttagttg gcacttctag gctagttaat
    17101 cttagaaatt gaacacggac tacctgcctc ttgaaaaaag atgcctgtct tgcctttcgt
    17161 ggaaagtcag tgccattccc tatttttcag gggcagctaa ggtcctttgt atcttgataa
    17221 ttgaacacgc ctggaaccct gtgtgaaaaa gatagttctt ccaagaagta gacacttctt
    17281 gatcagaatt cctattttca ctttgtgttc ttacaggctt tgtattttgg acaggagtag
    17341 agatgagtat tcgagtaatt attgccggtt ttaagggaaa gatgggccag gctgcttgtc
    17401 agatggtatt gactgatcca gacttggact tggtagcagt tttggatcct tttgagtctg
    17461 agtcagaatg gcagggtatt cctgttttca aggataaggc tgatttagct ggttttgaag
    17521 cggatgtctg gatagatttt actactccag ctgttgccta cgaaaataca cgttttgctc
    17581 ttgaaaatgg ctttgctcca gtagttggaa cgactggttt cacgagtgaa gaaattgcag
    17641 agctaaaaga attttctcgt gcccaagact tgggtggcct gattgcccct aactttgcct
    17701 tgggtgctgt cttactcatg caatttgcga cgcaggctgc caaatatttc ccaaatgtgg
    17761 agattattga gctccatcat gacaagaaaa aggatgctcc gagtggaaca gccattaaaa
    17821 cagctgagtt gatggcagag gttcgagagt caattcagca aggtgcagca gatgaggaag
    17881 agctgattgc tggtgctcgt ggtgctgact ttgatggtat gcgcatccac tcagttcgtt
    17941 tgccaggctt ggtagctcat caggaagtca tctttggcaa tcagggagaa gggttgaccc
    18001 tccgtcatga ctcctatgat cgcatctcct tcatgacagg agtcaatttg ggaattaaag
    18061 aagttgtcaa gcgtcatgag cttgtctatg gattagaaca cttattatga gattaacgca
    18121 aatgccttct gaatttcaga aggctttacc agtattagaa aaaattaaag aagcaggctt
    18181 tgaggcttat tttgttgggg gctctgttcg agatgccctt ctccatagcc ctatccacga
    18241 tgtggatatt gcgacgtctt cttatccaga agagaccaag cagatttttc cgcgaacagc
    18301 cgatatcgga atcgagcatg gaaccgtctt ggtcttagat ggggatgagg agtatgaggt
    18361 aacaaccttt cggacagagg atgtctatgt ggactatcgc agacccagtg cggtttcctt
    18421 tgtgcgctcg ctagaagaag acctcaaacg ccgtgatttc acagtcaacg cctttgcctt
    18481 ggatgagaca ggagaaatcg ttgacttgtt ccatggttta gaagatttgg aaaagcaagt
    18541 cttgcgagca gttggagtgg ctagtgagcg tttcaacgaa gatgctttac ggattatgcg
    18601 tggtttccgt tttcaggcta gtcttggttt tgcacttgag ccagaaacat ttaaagctat
    18661 gaagaccttg acgccgcttt tggagaaaat ttctgtagag cgtaccttcg tcgaatttga
    18721 taaactcttg ctggctccat tttggagaag gggcttggct tccatgattg agagtcaagc
    18781 ttatgactat ctccctgata tggcatctag ccaggacaag ctcaacagac tgtttgattt
    18841 ggagactgat tttacttttg aatcctctga acaagcctgg gcggctttac tatgggcttt
    18901 ggagattgaa aatgcgcagt catttttgaa atcttggaag acctcacgcc agtttgccaa
    18961 gcaagttcag gatttgctga ttattttggc tctgcgtgaa aatggagaat tgagcaagcg
    19021 agattgttat cgctttgaca tagatttgct tttacaggct gaaaatcttc gtcaagctca
    19081 aggaaaagaa gtcaacccac aagccatcac agaaaaatac caaagcttga ctatccatga
    19141 caagaaagag attcagatta atggcgatat tttgatcaag gaatatggct atcagccagg
    19201 cccagacttg ggagagattt taacagagat tgagtttgcc attgtcgatg gagaattgga
    19261 gaataatcgt gaagccatcc atgcttacct gagggagaaa aaatgagtga ttttatcgtt
    19321 gaaaaactaa gtaaatccgt tggtgacaag accgttttta gggatatttc ctttattatc
    19381 catgacttag acagaattgg tttaatcggt gtcaatggga ctggcaagac cacccttttg
    19441 gacgtccttt ctggtgtttc tggatttgat ggggatgtca gtcctttttc agctaaaagt
    19501 gattaccaga ttggttattt gactcaggat cctgattttg atgatagaaa gacagttttg
    19561 gatacggttc tatctagtga actcaaggaa atccagctca ttcgtgagta tgaattgatt
    19621 atgctcgact atagtgagga caagcaggcg cgtttggaac gtgtcatggc agagatggac
    19681 tctctccaag cttgggaaat cgagagtcag gtcaagaccg ttcttaacaa attgggcatt
    19741 caagacttat ctactcctgt tggggaattg tcaggtggtc tgagaagacg ggtacagttg
    19801 gcacaagtct tacttggcaa ccacgacctt ttgcttttgg atgagccgac caaccatctg
    19861 gatattgcga ttattgagtg gctgaccctc tttttgaaaa attctaagaa gaccgtcctt
    19921 tttatcactc acgatcgtta tttcttagac gctttgtcaa cacggatttt cgagttggag
    19981 cgagcaggct tgacagaata tcagggcaat taccaggact atgttcgcct taaggcagaa
    20041 caggacgagc gcgatgcagc tctacttcat aaaaaagaac aactctacaa acaagaactg
    20101 gcctggatga gcagacaacc gcaggcgcgt gcgaccaagc aacaagctcg tatcaatcgt
    20161 ttccatgatc tgaaaaagga agtttcaggc agtagtgctg agacagactt gactatgaac
    20221 tttgaaacca gtcggattgg gaagaaagtc atcgagtttc aggatgtttc ctttgcctat
    20281 gaaaataagc ccattttgca agattttaat ctcttagttc aggctaaaga ccgtattgga
    20341 attgttgggg acaatggtgt tggaaaatca accctactta acctgattgc aggaagtctt
    20401 gagccgacag caggacaagt tgtgattggg gaaactgttc gcatcgccta tttctctcaa
    20461 caaattgagg gtttggatga aagcaagcgt gtgatcaatt acctgcagga agtggcagag
    20521 gaggtcaaga ccagtggtgg ttctacgact tccatcgctg agttgctgga gcaattcctc
    20581 ttcccacgtt cgacgcatgg gaccttgatt gagaagttgt cagggggtga gaaaaaacgt
    20641 ctttatctcc tcaaactgct tttggaaaaa ccaaatgttc ttcttttaga cgagccaacc
    20701 aatgacctag atattgcaac tttgacagtc ttagagaatt tcttgcaagg ttttgcaggt
    20761 cccgttttaa cagtcagtca cgaccgctat ttcttggata aggtagcgac caagattctc
    20821 gcttttgagg atggcaagat tcgtcctttc tttggtcatt acaccgacta tcttgatgaa
    20881 aaagcttttg aaacagatat ggccaatcaa gtgcaaaagg ccgaaaagga aaaagtggtc
    20941 aaggttcgag aagacaagaa acgcatgacc taccaagaaa agcaggagtg ggcaagtatt
    21001 gaaggtgata ttgaaacctt ggaaaaacgt atcgctgcta ttgaagagga aatgcaggct
    21061 aacggctctg actttggtaa gctggctact ctccaaaaag aattggatga gaaaaatgaa
    21121 gcactccttg aaaaatacga acgctatgag tatctcagtg aatttgatag ttaagactat
    21181 tcgaaaatca aagtgcaaac tatgtcggct tcacattgcc gactcaagta cagcctgcga
    21241 ccggcttcct aatttgcact ttgattttta ttgagtataa acgaatagaa aaatctcgtc
    21301 tcatgaaagg atttctctat tcttttttgg tttcttgatg aaagatattt tgccaggttt
    21361 cgtggcgacg ccagatactg gtatggatga taccatctaa ctcatagcag atgagtttgg
    21421 ttttttgact gatggaagta atttgaatgt ccttgatagc agaatttaac tcttttttgg
    21481 tcttataagc ctctttatcc atctgctctc catcttggcg aatatagaca aaatcatcag
    21541 tcaagagttc ttctagttgg tttccttggt caatcaattg ttcacgcata agcaattttt
    21601 tatagacatt gtctaaaatt tcatcctcaa ggataggtgc tggttcgata tggacatcgg
    21661 tatcaaagac gccaaaacgc tcctccagca tagactcgac ctgatccgcg atttcatggc
    21721 tttcaaaaac agacaagtca ggattcatct ctagtgtaat atccaggtag atgttgctac
    21781 cgtaggtgcg acctctttgc gatttgacct tgctgatttt gggaatttcc atgatagcct
    21841 tttggtagtc ctcgagcagg cggtcgtcaa agccatctga aagactaaag gaagactcga
    21901 tgaagatatc ataggcagtc ttcaagataa agaaagtgat gatgatagca accagtttat
    21961 ccacaatcgg ataattgaaa ctactagcta ggatggcaat ggcggttcca agtgaggtaa
    22021 cagcgtcaga aagattgtcc ttagcagctg ccttcagcgc cttggagttg gatttcttac
    22081 tgaggcgagt attgtagaga tagaccacaa acataatcgc tgcagaaatg attcctagag
    22141 ttgcaccaag aggatcaatg accgtttctt cccgactgag aatcttttga atggtatctc
    22201 ttagaacatc gaaaccgaca tagaacatga tgatagaagt gatcaagctt gccaaatctt
    22261 caatcttcca atgaccaaaa cggtggtcac ggtctgcagg ctggcgcgcc atccgaatcc
    22321 cgattaagag ggctacattt ccaatgatgt ccgatacgtt attaaaacca tcggccacca
    22381 aactggatga atgaaggaga tgaccagctg ctaatttggc tgcagacaag atcaaatagg
    22441 tcgaaatact gataatggct ccacgctcag ctaacttgag atttgagata gattgcttca
    22501 ttcaacttcc tttctagtca catagcattc taccattata ctggttttca aggaaaaatt
    22561 caaataaggc agtttttact ctttgaaaac tccccaaaat ttggtataat ggtactactc
    22621 aagggagtag ctggcagaaa cctgtgatag tgtcgtcatt ccgaatttta tgctgaaaag
    22681 tatgctttcc ggccctatct taaacagcga gacttgttat gattaacagg tctctttttg
    22741 tttgtcgtga aaagatatga cagaaagaga gtctgttttg cacacaatgt caaggaggag
    22801 acacatgtca aaagaacaaa aacgccaagc gttttatact cagagccctg aagaggtctt
    22861 gcaggctatg gatgcgaccg agcaaggttt gtcatcaagt gaggcggaaa agcgccttgc
    22921 cgaatttggg cacaatgaac tcgaagaagg cgagaaacga tcaatcttgg tcaaattcat
    22981 cgagcaattt aaggatttga tgattatcat cctagttgcg gcagcaatct tgtcagtcgt
    23041 gacttctggt ggggaagata tcgcagatgc cattatcatc ctagctgtgg ttatcatcaa
    23101 cgctgccttt ggtgtttacc aagaaggaaa agctgaagaa gctattgaag ccctcaaatc
    23161 catgtctagt ccagttgccc gcgttcttcg tgatggacac atggcagaga ttgactctaa
    23221 agaattggta ccaggcgata tcgttgccct tgaagcaggt gacgtggtac cagcggacct
    23281 acgtttgata gaagccaact ctcttaaaat tgaagaagca gccttgacag gtgaatctgt
    23341 accagtcgaa aaagacttgt cagtcgagct tgcgacagat gctggtattg gtgaccgtgt
    23401 caacatggcc ttccaaaact caaacgtaac ctatggtcgt gggatgggtg ttgttgtcaa
    23461 tacaggtatg tatactgaag ttggtcatat tgctggtatg cttcaagatg cggatgagac
    23521 tgatacacca ctcaaacaaa acttgaacaa cctttctaag gtcttgacct atgctatctt
    23581 ggtcattgcc cttgttactt ttgtagtggg tgtcttcatt caagggaaaa atccacttgg
    23641 tgagttgttg acttctgttg cccttgccgt tgcagccatt ccagaaggac ttcctgctat
    23701 cgttaccatc gttctttctc ttggtactca agttttggcc aaacgacatt ccatcgttcg
    23761 taagttgcca gcagttgaaa cacttggttc aactgaaatc atcgcttctg ataagactgg
    23821 tacgctgact atgaacaaga tgacagtcga aaaagtcttc tacgatgcgg ttctacatga
    23881 ctcagctgat gatattgaac taggtcttga aatgccacta cttcgttcag ttgtcttggc
    23941 caatgatacg aaaatcgatg tggaaggtaa cttgattggt gacccaaccg aaacagcctt
    24001 tatccaatat gccttggaca agggctatga tgtcaaaggt ttcttagaga aatatcctcg
    24061 tgtagctgaa ttgccatttg cctctgaccg taagctcatg tcaacagttc acccattgcc
    24121 agatggtcgt ttccttgtag cagtcaaggg tgcgccagac caactcttaa aacgttgtct
    24181 tcttcgtgat aaggctgggg atattgctcc gattgatgag aaggttacaa atctcattca
    24241 tacaaacaat tctgaaatgg ctcatcaagc cttgcgtgtc cttgcaggtg cttataagat
    24301 tatcgatagt attccagaaa atctcacttc tgaagagctt gaaaatgatt taatttttac
    24361 tggtttgatt gggatgattg accctgaacg tcctgaagcc gctgaggctg ttcgtgtggc
    24421 taaggaagcg ggaatccgtc cgattatgat tacaggtgac caccaagaca cagcggaagc
    24481 cattgccaaa cgtttgggaa tcattgacgc aaacgataca gaaggtcacg ttttaactgg
    24541 tgctgaactc aatgaactgt cagatgaaga atttgaaaaa gtcgttggtc aatactctgt
    24601 ttatgcccgt gtgtctccag aacacaaggt tcgtatcgtc aaggcttggc aaaaacaagg
    24661 taaagtcgtt gccatgacag gtgacggtgt caatgacgcg ccagctctga aaacagccga
    24721 tatcggtatc ggtatgggaa tcactggtac agaggtttct aagggggctt ctgatatgat
    24781 tcttgcagat gataactttg cgactattat cgtcgcagtg gaagaaggac gtaaggtctt
    24841 ctcaaacatt caaaagacta ttcagtacct actttctgct aatactgctg aagtattaac
    24901 catcttccta tcaaccttgt ttggttggga tgtcttgcag ccggttcatc ttttgtggat
    24961 caacttggta acggatacct tcccagctat cgctcttggt gttgaacctg cggagcctgg
    25021 tgtcatgaat cataaaccac gtggacgcaa ggcaagcttc ttctcaggtg gtgttttgag
    25081 ttctatcatt tatcaaggtg tactccaagc agctcttgtt atgagtgttt atggccttgc
    25141 gattgcttac ccagttcatg tgggtgacaa tcatgctatt catgcagatg ccctaacgat
    25201 ggcctttgca acccttggtt tgattcagct cttccatgcc tacaatgtca agtctgtcta
    25261 ccaatccatc ttgacagttg gcccattcaa gtctaagacc tttaactggt ccatcttggt
    25321 atcctttatc cttctcatgg caacaatcgt tgtagaaccg cttgaaggaa tcttccacgt
    25381 aaccaaacta gacttgtcac aatggggaat tgttatggct ggaagcttct caatgattat
    25441 catcgtcgaa atcgttaagt ttattcaacg caaactcggt tttgacaaga atgcgattta
    25501 agggaggttt cggatgtcag catatcaatt accgaccgta tggcaggatg aagctagtaa
    25561 tcaaggagct tttacggggc taaacagacc aacagcaggt gcccgtttcg aacaaaactt
    25621 gccaaaagga gaacaagctt tttagcttta ttcactggga acaccaaatg gtgtgaaggt
    25681 tactatctta ttggaagaat tactagaagc tggttttaag gaagcggctt acgacttgta
    25741 taagattgct atcatggatg gggatcaatt cggctcagac tttgtgaagc tcaatccaaa
    25801 ttccaagatt ccagccttat tggaccagtc aggtactgaa aacgtaagag tctttgagtc
    25861 tgctcatatt cttctttacc ttgctgagaa atttggagcc tttttaccaa gtaatcctgt
    25921 ggaaaaggta gaagttttga attggctatt ctggcaagca ggtgcagcac cttttctagg
    25981 tgggggattt ggacatttct tcaattatgc tcctgaaaaa ttggaatatc ctattaaccg
    26041 ttttacgatg gaagtgaaac gccagttgga tttattggat aaggaattgg ctcagaaacc
    26101 ttatattgca ggcaatgact atacgattgc agatattgct atctggtctt ggtatggaca
    26161 gttagttcaa ggaaatcttt accaaggttc tgcaaaattc ttggatgcct caagttatca
    26221 aaatctagta aaatgggcag aaaaagttgc caatcgtcca gctgttaagc gtggcttgga
    26281 agtaacttat acagaaatta aatagcaaaa aagtcatctt cggatggctt tttgctacag
    26341 ttgagggaaa gggtttgcag ttatcggctt ttgtgctata attgctataa tatagtaaaa
    26401 agcaagagga gtgtgaggaa gtggaaaaga aaattgtgaa ggatatctta tttttatctc
    26461 aagtgtctca gccggcaagt caggaggacc tttatcttgc cagagatttg caggatacac
    26521 tcttagcaaa tcgtgatacc tgtgttggtc tagctgccaa tatgattggg gtgcagaagc
    26581 gcgtgattat ctttaatctt ggcttggttc ctgtggtcat gtttaaccca gtgcttctgt
    26641 cctttgaagg atcctatgag gcagaagaag gctgtttgtc cttggtaggt gtgagatcaa
    26701 ctaagcgtta tgaaaccata aggcttgcct atcgtgacag caagtggcag gaacagacca
    26761 ttaccttgac aggcttccca gctcagattt gccagcatga gctggatcac ttggaaggac
    26821 gaatcattta ggaggaaagc aaatgaaacg aatagtcttt gaacttattt ttatcgcaac
    26881 gacctggtat atctttttac cgccccttaa cctgaccagc tgggaatttc tcttcttcct
    26941 ctgtgggcat ttgttagttg tggcaatatt atttggcttt ggcaagggga taaaccttgt
    27001 caaaacggtt catgtgcgcc acggtaaggc ggaagctgcc ttaaatcttg agggtttcaa
    27061 aatcaatcgg ttagggaaaa ttctgttagc ttcgattgga ggaattcttc tcttggcagc
    27121 tttggtttcc ttggtaactt ccagcatgtt tcaggctaaa aattatgcca atgtagtcac
    27181 ggttacggaa aaagacttta ctgaatttcc taagagtgac accagtaagg ttcctatcct
    27241 agatagaagt actgctgaaa aaattggaga ccgctacttg ggttccctaa ccgataaggt
    27301 gtcgcaatac gtagcggcag atacctatac ccaattgaca attgatggga aaccttatcg
    27361 ggtcacacca ctagaatatg cagaccctat caaatggttt aacaatcaag ccaagggaat
    27421 cggtgagtat atcaaggtgg acatggtaac tggaaatgcg gattttgtgg acttgaagac
    27481 accaatcaag tattcagact cggagtattt taaccgtgat gtcaaacgtc acctgcgctt
    27541 gaagtacccg accaaaatct ttaaaactcc atcttttgag gtggacgatg agggcaatcc
    27601 tttctatgta gcaacggttt accaaaagca atttggactt gcggttcctc gtcctgcttc
    27661 agtcattatc ttggatgcta caaatggaga aaccaaggaa tacagcttat cagatgttcc
    27721 agaatgggtg gacaggatct atccagcaga ggaaaccatt gagcaaatta actacaacgg
    27781 caagtacaag gacggtttct tgaatgccat gatttccaag aaaaacgtga cccagactac
    27841 caagggctat aattacttgt ctatcggtaa tgacatctat ctctacacag gtgtgacgtc
    27901 ggctaatgcg gatgagagta atcttggttt catccttgaa aatatgcgaa caggagaaat
    27961 cactaagtat agcttggctt ctgcgacaga agaatcagcc cgtgaatcag cagaaggtgc
    28021 tgttcaggag aaatcctaca aagcaacctt cccaatcctc atcaacctca atgacaagcc
    28081 tctctacatc atgggcttaa aggacaatgc tggcttggtc aaagagtacg ccctggtaga
    28141 cgcagtcgag taccaaaatg ttatcgttgc tactacagtg gaagagatgc tcagcaagta
    28201 tgccaataaa aacgaccttg aaattgacaa tgcaacgaca gaaagcatca agggagtagt
    28261 agcagacctc aaatcagctg ttatcaaggg agacactgtc tacttcttta aagttgatgg
    28321 caagatatac aaggtcaagg cttcagtatc cgatgacctt ccttaccttg aaaatggtaa
    28381 aaccttcgaa ggtcaagtag gaaaagacaa ttatctcaag acctttaaag tacaataata
    28441 ctcttcgaaa atctctagct ctatctgcaa cctcaaaaca gtgttttgag cagcctgcgg
    28501 ctagtttcct agtttgctct ttgattttca ttgagtataa aaaaggttta tttaataaat
    28561 atatgttata ataaggtaaa ttaagcctaa tggaggacaa agaaatgggt ttttatttga
    28621 tggttgcttc aatgctactc ggactgctcg ctttgaagat aggtttttct cagttcaagg
    28681 agaaaaaaga taaattctta tctatcttaa caagtttagc tggcacagcc cttgttctcg
    28741 tggctgtctg gttaggatgg cccaaataaa tagaaaaacc tcggtcaaac cgaggttttt
    28801 caaatgaatt tcttggttgt ggctaaaaaa tatgctacac tatcaatatg aaaattttaa
    28861 tcccaacagc aaaagaaatg aacacagact tgccaagtat cgaggcaatt cctttaaaac
    28921 cagaaagtca gaccgtgctt gatgccttgg ctctctattc tgccagtcaa ttggagagtt
    28981 tctacaaggt atcggctgag aaagcggcgg aagaatttca aaatatccaa gctttgaaaa
    29041 ggcaaactgc tcaacactat ccagccttga aactttttga tgggcttatg taccgcaaca
    29101 ttaagagaga taagctgacc gaggcggaac aagattatct tgaaaatcat gtcttcatta
    29161 cctcggcttt gtacggtgtt gttccagcct tgtcacccat ggctcctcac cgtttggatt
    29221 ttttgatgaa attaaaagtc gctggtaaga ctttgaagag ccattggaag gcagtctatg
    29281 atgaagctct gaagaaggaa gaagtgattt tctctctctt gtcatcagag tttgagactg
    29341 tattttctaa ggaaatcaga gcaaagatgg tgaccttcaa attcatggag gatagaggcg
    29401 gtcagctgaa gattcactca actatctcca agaaagcgcg cggggccttt ctaacagctt
    29461 taatagaaaa tcaagtacaa actgtggggg aagcacgtcg cttgaacttt gctggatttg
    29521 tttaccgaga agatttgtca caaccacagg gattggtttt tgtaaaggaa gtatagaaat
    29581 cagatatttt gtatttggca atatgtatca tcagctgttt tagtttataa atgagaaaaa
    29641 aagcgaacaa gctagtcttc tgagtgtcag aaaagtagtc ttgttcgctt ttattatgtt
    29701 atactcttcg aaaatctctt caagccacgt cagcgtcgcc ttaccgtata tatgtcactg
    29761 acttcgtcag ttctatctac aacctcaaag cagtgctttg agcagcttgc ggctagcttc
    29821 ctagtttgct ttttgatttt tattgagtgt tagttacttg atagcttcga ccaagtcttg
    29881 agcttgtttt tcaagtgagt ttaggactgt ttcttcaaga accaattttc cgtctgccca
    29941 ggcagagtca ttaacacgtg cagcagtgaa atcaccaacg ccttgtgtac ggataaatgg
    30001 caagaggtct ttgtagatag cgaaaagttg atcgtgccct gcattggcta cagatgatac
    30061 tgtgacaaac ttgtcttgaa gggcagaaac gccacgtgta tcagacaagt caagggcacg
    30121 agatagccag tcaagcaagt ttttcactgt accagggata gagaagttgt agactggaga
    30181 gaaaatccag atagcatccg caacgagaac tgcttcacga gcagcagcta cagctggatg
    30241 tgttggaact tccaaatctt ggctgaagag aggaagggct gaataatcaa ggtagctaac
    30301 ttccgcttta ccagcaagtg ctttctcagc ttcgagcgcc atttggtggt tgaaagaacc
    30361 ttgacgtagt gatccgacga taaataatac ttttttagac atgatgtgtc tcctttttct
    30421 taaaatttcg agaactctct cgttgttata aaatatatta caacaaaaca aaagaacttg
    30481 caagaaattt gctcagagtt tatttgaaaa ttgtaaaatt tcggaaactc tagtcatttg
    30541 tgactgaaaa agtaagaact gtttcacttt caaaatcggt tttgtttgaa gggagaaata
    30601 gaattggttg ctcaaagcac agctttgagg ttgcagataa agctgacgtg gtttgaagag
    30661 attttcgaag agtattctta tattcacttg taataaagct taatagatgg taaaatagac
    30721 gagtgaaaaa aagacaaaac aaagcaaaaa ataatctact gtggcagtat ggtctaggga
    30781 tgacgatttt gtttgtggtt atcagtgctt cctttctgta tatggtttct cttagcatga
    30841 aaccctatca aacagctaaa agtgaaggag aaaaattagc tcagcagtat gcaggattag
    30901 agcaggctga tcaggttgat ttatacaatg gcttggaatc ttattacagc gttcttggtc
    30961 gtaataaaca gcaagaagcg cttgctgtcc tgattggtaa agatgaccat aagatttacg
    31021 tttatcagct aaatcagggt atttcacaag aaaaagcaga aacggtttct aaggaaaagg
    31081 gagctggcga gattgacaag ataacctttg gtcgttatca agacaagcca atctgggaag
    31141 ttaagtcagg atctgatttt tatctagtag attttgaaac aggagcattg gtcaacaagg
    31201 agggcctatg aaattatcca accgtgtttt agaaatggaa gaaagtgtga ctttggctgc
    31261 tggagcccgt gccaaagcac tgaaggctga gggtagagat attttgtctc taaccttggg
    31321 tgagccagat ttcactactc ccaaaaatat ccaagatgcc gccattgcat cgattcgaga
    31381 tgggcgtgct tctttttata cagtaacctc aggtctgcca gagcttaagg cggtggtcaa
    31441 tagctacttt gagcgctttt acggctattc tgtagcgtca aatcaagtga cagtcgctgc
    31501 gggagccaaa tattctctct ataccttctt tatggctgtg gtcaatccag gtgatgaagt
    31561 catcatccca accccatact gggttagcta tggagatcag gtcaagatgg cagaaggcgt
    31621 tcccgtcttt gtttctgcta aggaagacaa tcactttaag gtgaccgtag agcagttaga
    31681 agcagcttgc actgacaaga ccaaggtttt ggtgctgaat tcgccatcta atcccacagg
    31741 tatgatttac acccgtgagg aactcttggc aattggaaac tgggctgtag aaaatgatat
    31801 tctcatccta gcagacgata tctatggccg cttggtttat aatggtcatg agttcacacc
    31861 gatttctagc ctatcggaag cgattcgcaa gcaaacagtg gtcatcaatg gtgtgtctaa
    31921 aacctatgcc atgactggtt ggcggattgg ttatgccgtt ggggaagcag acattatcgc
    31981 tgccatgtcc aagattgcag gtcaaacaac ttcgaatccg tcagcagtag cccaatatgc
    32041 agcagttgag gctctatcag gtgagcaaga tactgtagaa agcatgcgtc aggcctttga
    32101 ggaacgtctt aataccatct atcccctcct tgcagaagtg ccaggatttg aagtggtcaa
    32161 accgcaaggg gccttctatc tcttcccaaa tgtcaaaaag gccatggaga tgaaaggcta
    32221 cacggatgtg acagacttta caactgttat cttagaagaa gccgaagtgg ccttggtaac
    32281 aggagctgga tttggagcac cagaaaatgt ccgcctcagc tatgcgacag acctagacac
    32341 gcttaaagaa gcagtcgaac gcttgaaagc atttatgggt agtgagaatg attgatcatt
    32401 ttgagattaa ggtaaaggat ttacaaattt caggaggatt ttataggagt tttctcgctc
    32461 ctttggacta taaattggct tttaagacta gttctctaat tagttttcta tccccgaaca
    32521 gccctcatcc tggtggtgat ttttggctga ctcaaggaac acaagatcct gttcattttg
    32581 cttttttagc agaaaataag gaagaagttt aggcttgtta tgaggctggc ttagaggcag
    32641 gtgggcgaga caatggggct cctggttatc gaagtgagcg tccgatttac tatgctgctt
    32701 ttatgattga cctggatggg aacaatatag aggtggtttg ccataaagaa taaaaaagaa
    32761 aaggaaaaat aatgacaaaa cgtgtaacga ttattgacgt aaaagactat gttggtcagg
    32821 aagtgacgat tggcgcttgg gttgccaaca aatcaggaaa agggaaaatc gctttcttac
    32881 aattgcgtga tggaacagcc ttctttcaag gtgtggcttt taaaccaaac tttgtcgaaa
    32941 aatttggtga agaagtggga cttgagaagt ttgatgttat caaacgcttg agccaagaaa
    33001 cgtctgttta tgtgacaggt attgtcaaag aggacgaacg ttctaaattt ggctatgagt
    33061 tggacatcac agacatcgaa gtgatcggtg aatctcaaga ctacccaatc acaccaaaag
    33121 aacacggaac agactttttg atggataacc gtcacttgtg gctacgctct cgtaagcaag
    33181 tagctgtgtt gcaaatccgt aacgctatta tctatgcaac ttatgagttc tttgacaaga
    33241 acggttttat gaagtttgac agcccaattc tttcaggaaa tgcggcagaa gattctacag
    33301 aactctttga aactgactac ttcggaacgc cagcctactt gagccaatca ggtcagcttt
    33361 acctagaagc aggggctatg gctcttggtc gtgtctttga ctttggtcca gttttccgtg
    33421 ctgaaaaatc aaaaacacgc cgtcacttga ctgagttctg gatgatggat gctgagtact
    33481 catacttgac acatgatgag tcgcttgact tgcaagaagc ttatgtgaaa gctcttctac
    33541 aaggtgttct tgaccgcgcg cctcaagcct tggaaacctt ggaacgtgat acagaactct
    33601 tgaaacgcta cattgcagag ccattcaaac gtatcactta cgatcaagcc attgacctct
    33661 tgcaagagca tgaaaatgat gaagatgctg actacgagca tcttgagcat ggtgatgact
    33721 ttgggtcacc acacgaaact tggatttcaa accactttgg tgtgccaaca tttgtcatga
    33781 actatccagc agccatcaag gccttctaca tgaaaccagt tcctggaaat ccagagcgcg
    33841 tgctttgtgc agacttgctt gctccagaag gctatggaga aattatcggt gggtctatgc
    33901 gtgaggaaga ttacgatgcc cttgtcgcta agatggatga acttggcatg gatcgtacag
    33961 aatatgaatt ctaccttgac cttcgtaaat acggtacagt tccacacgga ggatttggta
    34021 tcggtatcga acgtatggta accttcgcag caggaacaaa acatatccgt gaagctattc
    34081 cattcccacg tatgttgcac cgtatcaaac cataagaaca ggaaaacgcc catgtggcgt
    34141 tttttcgaat tctagtgttt ttacttgcgt aaaaaaattt tttctagtat aatagtttat
    34201 tgtgagcgaa cctcacttac cccttgcaaa gtcttggggt cattagacca aaaggaggaa
    34261 catatcaatg gctaaatacg aaattcttta tatcattcgt ccaaacattg aagaagaagc
    34321 taaaaacgct ttggtagcac gttttgactc tattttgact gacaacggtg caactgttgt
    34381 tgaatcaaaa acttgggaaa aacgtcgtct tgcatacgaa atccaagatt tccgtgaagg
    34441 actttaccac atcgttaacg ttgaagcaaa tgacgatgca gctcttaaag agtttgaccg
    34501 tctttcaaaa atcaacgctg acattcttcg tcacatgatc gtcaaaattg acgcgtaaga
    34561 aggtaaacta tgattaacaa tgttgtactt gtagggcgta tgacacgtga cgctgaattg
    34621 cgttataccc catcaaatgt agcagttgcg acttttactc ttgcagtaaa ccgtacattt
    34681 aagagtcaaa atggtgaacg tgaggctgat tttatcaatg tcgttatgtg gcgccaacag
    34741 gctgaaaatc ttgctaactg ggctaaaaaa ggctcactta tcggggtgac aggtcgtatc
    34801 cagactcgta gttacgataa ccagcaagga caacgtgtct acgtgacaga ggtcgtggct
    34861 gagaatttcc aaatgttgga aagccgtagt gtgcgtgagg gtcacacagg tggagcttac
    34921 tctgcaccaa ctgcaaacta ttcagcacct acaaattcag taccagactt ttcacgtaat
    34981 gaaaatccat ttggagcaac aaacccattg gatatttcag atgatgattt accattctaa
    35041 tggacaacta aatttataaa ggagaaaaaa catggctcaa caacgtcgtg gcggattcaa
    35101 acgccgtaaa aaagttgatt acatcgcagc aaacaaaatt gaatatgttg attacaaaga
    35161 tactgagctt cttagccgtt tcgtttcaga acgtgggaaa atccttcctc gtcgtgtaac
    35221 aggaacttca gctaaaaacc aacgtaaagt aacaacagct atcaaacgcg ctcgcgtaat
    35281 ggctttgatg cctttcgtaa acgaagatta aagaaaagac ccagttgggt ctctttttca
    35341 tgagcttgaa aacaagaaag cgaattaaga ccccaacggg tcttttttca caagctttga
    35401 aatgagagag cgagttaggc tcagtggctc cctttttacg agtttgaaaa caagagaaca
    35461 cattaaaagc acgatggagc tttttcataa atatgtctct tttctcatcc aagaaattaa
    35521 aataaaaatg ataaactttt tgaaaatagg ggatttgcct actttatgat ataatggaaa
    35581 gagttagatt gaaagaggta gtgagatgga cgcgaaatta agatacaagg caaagaagat
    35641 caagattgtc ttttttgata ttgatgatac attgcggaat tcaaagacag ggtttattcc
    35701 aactacaatt cccactgttt ttaaacagtt gcgtgaaaaa ggaattttaa caggaatcgc
    35761 ctctggacgt ggcatttttg gtgttgttcc agagattcgt gatctcaagc ctgacttttt
    35821 tgtaactttg aatggggctt atatcgaaga taaaaaaggt caggtcattt atcagcatca
    35881 gattgaaaag tcagatgttg aggagtatat ctcttgggct aagcaagaag gaattgagta
    35941 tggcttggtt gggagtcatg atgccaagtt gtcgactcgc accgatatga tgagtgaagc
    36001 tatcaatcca atttatcccg acttagatgt agatcccgat ttccatgaaa aagaagatat
    36061 ctatcagatg tggacttttg aagataaggg agatgacttg cacttgcctg acagtctctc
    36121 agacaaactt cgcatggttc gttggcatca acattcgtct gatattgtgc cgatttcagg
    36181 ctccaaagcg acgggggtgg aaaaggttgt ggaacacctt ggcttgaaac cagagaaggt
    36241 catggttttt ggagatggtc tcaacgactt ggaactcttt gattatgctg gaatcagcgt
    36301 tgccatggga atttctcatg ataaaatcaa agaaaaagca gattatatta caaaaacatt
    36361 agaagaagat ggcatttttg ctgccttaga agtatttggt atggtagaaa aagaattgca
    36421 ttttccacaa gtagacattg aaacagtaga aggtcctctt gcgactatta agaccaatca
    36481 cggagactta cgtatcaagc ttttccctga acatgctcct aaaacagtgg ctaactttgt
    36541 atctctttca aaagatggct actatgatgg tgtcattttc caccgtatta tcaaggactt
    36601 tatgatccaa ggtggagacc caactggaac tggtatgggt ggccagtcaa tctacggcga
    36661 atcttttgag gatgaattct cagaagagct ttacaatatc cgtggtgctc tttccatggc
    36721 aaatgctggt ccaaatacca acggcagcca gttctttatc gtgcaaaacc aacacctacc
    36781 ttattctaag aaagaaatta ctcgtggtgg ttggccagaa ccgattgcag aaatctatgc
    36841 caatcaaggt gggacacctc acctagaccg ccgtcacacg gtttttggtc agttagctga
    36901 tgaagcatct tacgctgtct tggatgccat tgctgctgtt gagacaggag ctatggacaa
    36961 gccagttgaa gatgttgtaa ttgaaactat tgaaatcgag gactaagatg aaaatcggtg
    37021 ataagctaaa ggggcgtatt acagggattc agccctacgg tgcctttgtt gagttagaga
    37081 cgggtgatac ggggctgatt cacatttcag aaattcggac aggatttatt gaaaatattc
    37141 atgaaacctt gaaagtcgat gaagaggttc aagttcaggt agtggattta gatgaattta
    37201 cggggaaagc cagtctttct atccgcactt tggaggaaga aaagtaccag tttcctagac
    37261 ggagacgctt ttcaagcgac cgctttaact acggttttgc tccctttcga cgaatgctac
    37321 caatctggac tggagaagcc ctgcaccatt taaaaaagaa gaagtagttt agagttttat
    37381 attttttgta atgttaatat gaatttgcta taataggacc atggaagaag aacaattatt
    37441 aaaatcagga gagcgcatta accagctctt ttcgacagat atcaaaatca ttcaaaatag
    37501 agaggttttt agctattcgg tggatagtgt tctcttatca cgatttccac gttttcctaa
    37561 gaaggggttg attgtggatt tctgtgctgg gaatggagca gtggggcttt ttgctagcac
    37621 tcgtactcaa gcacagatat tgtctgttga gattcaggag cgtttggcgg atatggctga
    37681 acgctctgtc cgtttgaatg gattagagga gcaaatgcag gtcatctgcg atgatttgaa
    37741 aaatatgcct gctcacattc aggggagtaa ggtggatatg attttgtgta atccacccta
    37801 tttcaaggtg gatccttatt ctaatctgaa cgagagtgaa cattatctct tggctcgaca
    37861 cgaaatcacg actaatttgg aagaaatctg tcgtagtgcc cagagtattc tcaagtctaa
    37921 tgggcgtttg gccatggttc atcgtcctga tcgacttctg gatattttgg atacgttaaa
    37981 acgacataat ctagccccta agcgcctgca gtttgtttat ccaaaaagag aaaaggaagc
    38041 caatatgctt ttgattgagg cgatcaagga tggctcaaca agtggcttta aggtcttacc
    38101 tcctttgatt gtccacaatg atgatggctc ttatacgccc gaaatcgaag agatttatta
    38161 tggatcataa ggcttatatg tacgttctgg agtgtcgtga tggatcctac tatataggct
    38221 atacgactga tatgagaaga cgcctcgcta tccacaatag tgggaaggga gccaaatata
    38281 cacgagcacg cttgccagtc aaacttatct atgctcaagg ttttgccagt aaggaagaag
    38341 ccatgtcggc tgaagctctt ctcaagcgta agaagaggcc acagaaggaa gaatttttat
    38401 ctgaaaatca agatagaaat ttactcagtt attttgaaga atcgtgggga gtcctttgac
    38461 tcctcttacc tttgtcaaaa agcgaaaaaa atgctacaat aaagaaaata aacaaaatga
    38521 ggtattatca tgtccaagat tctagtattt ggtcaccaaa atccagactc agatgccatc
    38581 ggatcatctg tagcttttgc ctaccttgca aaagaagctt acggtttgga tacggaagct
    38641 gttgcccttg gaactccaaa tgaagaaaca gcctttgtct tgaactattt tggtgtggaa
    38701 gcaccacgtg ttatcacttc tgccaaagca gagggggcag agcaagttat cttgactgac
    38761 cacaatgaat tccaacaatc tgtatcagat atcgctgaag tagaagttta cggtgttgta
    38821 gaccaccacc gtgtggctaa ctttgaaact gcaagcccac tttacatgcg tttggagcca
    38881 gttggatcag cgtcttcaat cgtttaccgt atgttcaaag aacatggtgt agctgtgcct
    38941 aaagagattg caggtttgat gctttcaggt ttgatttcag atacccttct tttgaaatca
    39001 ccaacaacac acccaacaga taaaatcatt gctcctgaat tggctgaatt ggctggtgtg
    39061 aacttggaag aatatggttt ggcaatgttg aaagctggta ccaacttggc tagcaaatct
    39121 gctgaagaat tgattgatat cgatgctaag acttttgaac tcaacggaaa taatgtccgt
    39181 gttgcccaag tgaacacagt tgacatcgct gaagttttgg aacgccaagc agaaattgaa
    39241 gctgcaatgc aagctgccaa cgaatcaaac ggctactctg actttgtctt gatgattaca
    39301 gatatcgtca actcaaactc agaaatcttg gctcttggtg ccaatatgga caaggtcgaa
    39361 gcggctttca acttcaaact tgaaaacaat catgccttcc ttgctggtgc cgtttcacgt
    39421 aagaaacaag tggtacctca attgactgaa agctttaatg cgtaagattt tgggtgtcag
    39481 ctcaaaatcg gaaagtctag tttgccttat atcgaaagga gtttcggctc cttttttcta
    39541 ggagtgaagt atgttagaaa atggcgattt gatttttgtg agagatgggt cagacatggg
    39601 acaggccatc cagacttcca caggtaacta tagccatgtt gccatttatt tggatgggat
    39661 gatttatcat gctagtggac aggctggtgt tgtctgtcaa gaaccggcag acttctttga
    39721 gtccaatcat ttatacgacc tctatgttta cccagaaatg gatatccagt cggtgaagga
    39781 aagagcttgc aaacatcttg gagcacccta caatgcttct ttctatccag atgcagctgg
    39841 tttttactgc tcccagtata tagcagaaat cctacctatt tttgaaacta ttcctatgaa
    39901 atttggagat ggggagcagg agattagtga tttttggagg gagtattaca tagaactagg
    39961 tctgcctgtt cctctgaacc aagctggtac caatcctagt cagttggcag catcgcctct
    40021 gttacaatgt aaagaaagga atcttcatga ttcagatttt taattcatct cgtttgacga
    40081 gacagccatt tttggagaat tgatccgcta tctggatcag tatgaggatg tgattctacg
    40141 ggaaattaag gctcaatttc cagatgttgc agttgataaa ctcatggaag agtatataaa
    40201 ggcaggcttg attctacgtg aaaataagcg ctattacctc aattttccta cgcttgaatc
    40261 acttgatagt cttgaactgg atcaagagat ttttgtcaga gaagctagtc cggtctatca
    40321 agccttgttg gagcagagtt ttgagacgga attgcgcaat caaatcaatg cagctatttt
    40381 agttgaaaag acggactttg cgcgcattaa aatgaccctg tccaattatt tttacaaggt
    40441 caaacagcag tatcctttga cagaaaaaca gcaggagctc tatgacattt taggagatgt
    40501 taatcctgag tatgccctca agtatatgac ggcttttttg ttgaaatttc tcaaaaaaga
    40561 ccagcttatg cagaaatgcc gtgatatctt tgtggacagt taggttgtct taggctatat
    40621 tgtgcaaaat gaagatggaa agtatgagtt ggctatcgat tttgataagg agaggttaac
    40681 tttctactta gcgtgatttc ttgtttctga gtacattgtt tgactttcct tagtattcgg
    40741 tataaactat atgtaaccgg taacacatat cggaataaac taaaggagac aatcatatgt
    40801 cacttgaaaa caaattggaa caagcaacag gcgctgtcaa agaaggtttt ggtaaagtta
    40861 ctggagacag caagacagaa cttgaaggag ctgttgaaaa aacagtttct aaggcaaaag
    40921 acgttgtaga agacgcaaaa ggtgctgtag aaggtgccgt tgaaggtttg aaaaacgttt
    40981 ttactaaata ataggaaaaa atcaagggtt tcattttccc ttgatttttt ctattcttat
    41041 aaataatttt ctgcgacggc tgtatctcct gggtaggatt ctttcttgcc ctggatgatt
    41101 tggtaacaat cggctccctt acccgcaata ataactgcat ctaattcgtg atttgtgata
    41161 gccattgccg ccttgatggc ttcttggcga tccgcaatct tctcaacagg atgattgatg
    41221 tagctactaa tttcatctgc aatggccatt gggtcttcat agttagggtc atcagcagtc
    41281 agaaagactt gaatctcagg gtgttgattg aggaggaggc caaagtcctt acgacgactt
    41341 tctcccttgt ttcctgttga tcccagaacc agagcaatct ttccggtttg atgagtttca
    41401 accacattga tgagtttttt cagactatcc ccattgtggg catagtcgat gaagaccttg
    41461 gctccatttt tctgagtgag gacttccata cgaccaggaa cgcgggttgc agcgatgcct
    41521 tttttgatat cctcaagact tgctccgaga cggagacaag caagtccagc agcaactgca
    41581 ttttcttggt tgaagttgcc aatgagttgg atatcataat ctccagcgag tttacccgta
    41641 gctgaaaatc taaaggcttt ggaattctcg atttggttat caaattggct accatagaaa
    41701 tcatggtctt gatcttcaac ctgttctttc aagactgaga agtggtccat gtcactgtta
    41761 atgatgactg ctcggctatt ttccatcaag agacgcttgt ggtagaaata gtcttcaaag
    41821 ctagggtgtt caatcgggcc gatatggtct aggctgatat ttaggaaaac tcccacatca
    41881 aaggttagac catagacacg tttgaccaga taggcttgac tggagacttc catgatgagg
    41941 tgggtacggt cattttgcac agcctgattc atcatgtcaa agaggtcaat actctcaggg
    42001 gttgtcaacg ctgacttaaa gaaagtctct ccatcaagag ttgtgttcat ggtcgacaac
    42061 atagcaggtc gatgcccttg agataagatg ttataggcga aataggctgc tgttgtctta
    42121 cccttagtac cagtaaaggc aaggagtttg agtttttcct gtggattacc atagaactcc
    42181 atggcaatca aactcatggc tttctttata tcgttcacaa tgatgacagg gataccgact
    42241 tcgtagtcct tttcagctat ataccaagct aagccttgtg ttatagcaga aagaaggtat
    42301 tcttttttaa aggcagcgcc ttttgcaaaa aaaagagtgt cttctgttac ttttcggctg
    42361 tcgtagctga tgctatcaaa aacaactttg ctgtagttgt agtggtaatg accttggtca
    42421 ataatttcgc gaaaaaggcc atctttcttt aaaatatcta atacagtttc aatttttatc
    42481 atactttcta ttgtaaaccg aaagtcgcaa atttacaagt gacaaggaaa agtttataat
    42541 ggaagataag gagtttttcc tagttatcaa aattgaatga ggaatctatg tcgcacgaaa
    42601 acaatcacca gcaggcccag atgttacggg ggactgcttg gctaacggct agtaacttta
    42661 tcagtcgcct actcggggct gtttacatta tcccttggta catctggatg ggggcttatg
    42721 cagctaaggc aaatggtctc tttaccatgg gttacaatat ctatgcttgg ttcttgttgg
    42781 tttcaacagc ggggattcca gttgcggtgg ccaagcaagt tgccaagtat aataccatgc
    42841 gagaagaaga gcatagcttt gccctgattc ggagcttctt aggctttatg acaggactag
    42901 gcctggtttt tgctttagtc ttgtatgtct ttgctccttg gctagcagac ttgtctggcg
    42961 tgggcaaaga cttgatccca atcatgcaaa gcttggcttg gggagtcttg attttcccgt
    43021 ctatgagtgt tatccgagga tttttccaag ggatgaataa cctcaaaccc tatgccatga
    43081 gccaaattgc tgagcaggtc attcgtgtta tctggatgct cctagcaacc tttatcatta
    43141 tgaagctcgg ttcaggagat tatctagcag ccgttaccca atcaaccttt gctgcctttg
    43201 tcggtatggt agccagtttt gcagtcttga tttatttcct tgcccaagaa ggttcactca
    43261 aaagagtctt tgaaacagga gataagatta acagtaagcg tctcttggtt gataccatta
    43321 aggaagccat tccttttatc ctgacagggt ctgccatcca gctcttccag attttggatc
    43381 agctgacctt tatcaatagt atgagctggt ttaccaacta cagcaatgag gacttggttg
    43441 tcatgttttc ttatttctca gccaatccta ataaaatcac gatgattttg atttctgtag
    43501 gggtttcgat tgggagtgtt ggtttgccac ttttgacgga aaactatgtc aagggggact
    43561 tgaaagcagc ttctcgtctc gttcaggaca gtctcaccct actctttatg ttcttgctac
    43621 cagcaacagt tggagtggtt atggtaggag aacctcttta tacggtcttc tatggtaagc
    43681 cagatagttt ggctctgggc ttatttgtct ttgcagtttt gcagtctatt attttaggct
    43741 tgtacatggt cttgtctcca atgcttcagg ccatgttccg caaccgcaag gccgttctct
    43801 attttatcta tggttctatt gccaagctag tcttgcaact acctaccatc gccctcttcc
    43861 acagttatgg tcctttgatt tcaacaacca ttgctctcat cattcctaac gtcttgatgt
    43921 atcgggatat ttgtaaagta actggtgtca agcgcaaggt gattttgaag cgaaccattt
    43981 taatcagttt gctgacccta gtcatgtttc tgttaatagg aaccatccag tggctgttag
    44041 gatttttctt ccaaccaagt ggacgtttgt ggagcttctt ttatgtagct cttgtcggtg
    44101 ccatgggggg tggactttat atggttatga gtctgcgtac ctatttgtta gataaggtaa
    44161 taggaaaagc ccaagcagat cgcctgcgag caaaatttaa gctttcgtaa ggtagcattt
    44221 ataaataaaa ggaataagtt gaacaatctt ttaggagaac ttgaaactta ttcctttttt
    44281 agc
//
LOCUS       DAVMGZ010000005        36944 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000005 DAVMGZ010000000
VERSION     DAVMGZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36944
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.005"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(686..1534)
                     /locus_tag="VYQ93_000198"
     CDS             complement(686..1534)
                     /locus_tag="VYQ93_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV3334957.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1686..1964
                     /locus_tag="VYQ93_000199"
     CDS             1686..1964
                     /locus_tag="VYQ93_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3334958.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1966..4029
                     /locus_tag="VYQ93_000200"
     CDS             1966..4029
                     /locus_tag="VYQ93_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV3334959.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(4107..5363)
                     /locus_tag="VYQ93_000201"
     CDS             complement(4107..5363)
                     /locus_tag="VYQ93_000201"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV3334960.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            5505..7970
                     /locus_tag="VYQ93_000202"
     CDS             5505..7970
                     /locus_tag="VYQ93_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV3334961.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLRENGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            8035..8946
                     /locus_tag="VYQ93_000203"
     CDS             8035..8946
                     /locus_tag="VYQ93_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV3334962.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            9176..9874
                     /gene="dapD"
                     /locus_tag="VYQ93_000204"
     CDS             9176..9874
                     /gene="dapD"
                     /locus_tag="VYQ93_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV3334963.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9942..11072
                     /locus_tag="VYQ93_000205"
     CDS             9942..11072
                     /locus_tag="VYQ93_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV3334964.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            11084..11623
                     /locus_tag="VYQ93_000206"
     CDS             11084..11623
                     /locus_tag="VYQ93_000206"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV3334965.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLTPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            11607..12284
                     /locus_tag="VYQ93_000207"
     CDS             11607..12284
                     /locus_tag="VYQ93_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV3334966.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(12351..13250)
                     /gene="galU"
                     /locus_tag="VYQ93_000208"
     CDS             complement(12351..13250)
                     /gene="galU"
                     /locus_tag="VYQ93_000208"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV3334967.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(13272..14288)
                     /locus_tag="VYQ93_000209"
     CDS             complement(13272..14288)
                     /locus_tag="VYQ93_000209"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV3334968.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            14500..14881
                     /locus_tag="VYQ93_000210"
                     /pseudo
     CDS             14500..14881
                     /locus_tag="VYQ93_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(15055..15480)
                     /locus_tag="VYQ93_000211"
     CDS             complement(15055..15480)
                     /locus_tag="VYQ93_000211"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3334969.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(15548..16198)
                     /gene="phoU"
                     /locus_tag="VYQ93_000212"
     CDS             complement(15548..16198)
                     /gene="phoU"
                     /locus_tag="VYQ93_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV3334970.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(16213..16965)
                     /gene="pstB"
                     /locus_tag="VYQ93_000213"
     CDS             complement(16213..16965)
                     /gene="pstB"
                     /locus_tag="VYQ93_000213"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV3334971.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(16967..17782)
                     /gene="pstA"
                     /locus_tag="VYQ93_000214"
     CDS             complement(16967..17782)
                     /gene="pstA"
                     /locus_tag="VYQ93_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV3334972.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(17775..18638)
                     /gene="pstC"
                     /locus_tag="VYQ93_000215"
     CDS             complement(17775..18638)
                     /gene="pstC"
                     /locus_tag="VYQ93_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV3334973.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(18756..19631)
                     /locus_tag="VYQ93_000216"
     CDS             complement(18756..19631)
                     /locus_tag="VYQ93_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV3334974.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(19798..21129)
                     /locus_tag="VYQ93_000217"
     CDS             complement(19798..21129)
                     /locus_tag="VYQ93_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV3334975.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(21130..21837)
                     /locus_tag="VYQ93_000218"
     CDS             complement(21130..21837)
                     /locus_tag="VYQ93_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3334976.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(21921..22223)
                     /locus_tag="VYQ93_000219"
     CDS             complement(21921..22223)
                     /locus_tag="VYQ93_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV3334977.1"
                     /translation="MTADEIWYFHAESPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            complement(22210..22431)
                     /locus_tag="VYQ93_000220"
     CDS             complement(22210..22431)
                     /locus_tag="VYQ93_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV3334978.1"
                     /translation="MNKSYFNPLAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINL
                     AGKGRALYTSIYFLLRRNESFTFSPIDSR"
     gene            22393..22952
                     /locus_tag="VYQ93_000221"
                     /pseudo
     CDS             22393..22952
                     /locus_tag="VYQ93_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23267..24958)
                     /locus_tag="VYQ93_000222"
     CDS             complement(23267..24958)
                     /locus_tag="VYQ93_000222"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV3334979.1"
                     /translation="MNTKELIASKLSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTIAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            25094..25540
                     /gene="argR"
                     /locus_tag="VYQ93_000223"
     CDS             25094..25540
                     /gene="argR"
                     /locus_tag="VYQ93_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV3334980.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            25591..28125
                     /gene="mutS"
                     /locus_tag="VYQ93_000224"
     CDS             25591..28125
                     /gene="mutS"
                     /locus_tag="VYQ93_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV3334981.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            28272..29966
                     /gene="patA"
                     /locus_tag="VYQ93_000225"
     CDS             28272..29966
                     /gene="patA"
                     /locus_tag="VYQ93_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV3334982.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLAG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(30296..30559)
                     /locus_tag="VYQ93_000226"
                     /pseudo
     CDS             complement(30296..30559)
                     /locus_tag="VYQ93_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            30757..32523
                     /gene="patB"
                     /locus_tag="VYQ93_000227"
     CDS             30757..32523
                     /gene="patB"
                     /locus_tag="VYQ93_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV3334983.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            32662..33351
                     /locus_tag="VYQ93_000228"
     CDS             32662..33351
                     /locus_tag="VYQ93_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV3334984.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            33355..33534
                     /locus_tag="VYQ93_000229"
     CDS             33355..33534
                     /locus_tag="VYQ93_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3334985.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            33521..34870
                     /locus_tag="VYQ93_000230"
     CDS             33521..34870
                     /locus_tag="VYQ93_000230"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV3334986.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            34994..36454
                     /locus_tag="VYQ93_000231"
     CDS             34994..36454
                     /locus_tag="VYQ93_000231"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV3334987.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            36468..36899
                     /locus_tag="VYQ93_000232"
     CDS             36468..36899
                     /locus_tag="VYQ93_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV3334988.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNE"
BASE COUNT        10760 a         7059 c         8125 g        11000 t
ORIGIN      
        1 atttattttg gattttattt gttattttga cctgcctcta tattggtaac catgatttgt
       61 ttactctcaa tcatcaagaa ttctcttttc gtggtagcgt ttggggtctg gtactggcct
      121 tatatcactt attgttcatt gataagtttg taatttcgaa tcgaaaataa agattagaac
      181 tatgattgac tggctacttt taatattgat tttggaggaa gattttgtct ctattattta
      241 ttattttaaa tttatttatt ttgtataaga tctattcttt gaggcgggaa aaatcgaaat
      301 acttgattta tacggcctat atcatatttg gggtaaatgt agtatatggt attcaatggt
      361 tattaagaga actgatttca actatttccc cttaatgtgt atggaaacta taacaaaacg
      421 cataagatta aggtttgtgt acctgttctt atgcgttttt ataatattga agattttcgg
      481 taagttttct acttttgcga agtctagttt atactcaatg aaaatcaaag agtaaactag
      541 ccgtaggttg ctcaaagtac agctttgagg ttgcagataa aactgacgaa gtcagctcaa
      601 aacatggttt tgaggttgta gatgaaactg acgaagtcag taaccatacg tacggcaagg
      661 cgacgttgac gtggtttgaa gagtattaga atgctttccc aaccaaaatc tctgcttcga
      721 tggtaatctc tgtcagttgg ctccagtcaa tcttgctctg gtcaacgtga aagaggagag
      781 gagtcatact gagtagggct tggagttgtt ctgctgtgat agtcttagtc agagaggcag
      841 tttgactaga taggatggta aagtgttcct ggaaatgctc cttgatatct tggttagaat
      901 actccttgtt tgtcagctgg tcctgtactc tttgacggat ttctttgagg tgattttcag
      961 ttgggataac ctttatcaag ataccgtctt tggataaaac gcgacgaaat tctccatagt
     1021 tggcaggtga gaagatatca agcagaatat ccatgttagc gtcttttata ggaaggcgtg
     1081 ccaagtcgcc aacgaaccaa ttgactgccc agttgggttc acttttagca gcgatttgga
     1141 ctgaatcttt ggagatatca aaggcataga aagttttttc agagtgactt tcttgtagtt
     1201 tgcgagaata gaatccttca ccacaaccga tatccaaaat tgttgtggta gtttttgagc
     1261 ttgcaagcaa gtcagataca gcatctaaga tagcttggta aaagccggct tctaggattt
     1321 gttgacggtt ttgaaaattt tccttgtcat agttagcaga ttgcttgatt tgaggtgcta
     1381 gattgacata gccaaatttc gccaagtcaa aagaatgacg gttgcagcac ttgaaattag
     1441 tctctaacag agtcagattt tcttgacaga taggacaggc aaaggcagtc gcagaagcaa
     1501 aacgctgaag tttgggcttg agatttgtat tcatagttta agtgtatcaa aagaggcaaa
     1561 tgaaagcaac tttaggaata agtagatggg agattcagta gaaataaaac taattctcca
     1621 atttatagaa tgaaattgct tttatatcta aattgctata taataatgat aaggaggaaa
     1681 taagtatgtt aaaagaagta ttaaccgttg caaaagttgc gaaaaaatct tcattatttt
     1741 tgggtggtgt cgcatttggt acccttggtt tgaaaatctt agcaagtaag gaagctaaaa
     1801 aaggttattc taaagctttg gctaaggctt acaagttgaa agacgagcta gatgcatctg
     1861 tttctgttgt gaagcaacat ggagacgatg tcttgcaaga tgccaaatat ttgtacgagc
     1921 aagagaaaaa agaagagcaa ttagatagcc ttataggtga ataatatgtc ttttaaagtg
     1981 ctacatagag gataccaaca tatccgacta tcatcttctt tttcacttac cttggatatt
     2041 caagactatc ttcgttcctt ggcgagagat gaaaagggga ttgagtctat ccagttttac
     2101 atggatcaac agcactttac tctacgcata aaagaaggct tttctgtatt agataatgca
     2161 gaagcctttt taaaaagaat tgataaaggg aaagtttctg agttgatgac tcttcccatt
     2221 cgtagagaag agagtgctta ttctattgtt tcaggtgcag cggttaagcg tgtacttttt
     2281 cgtagttttg tgccgtatcc tattcgctat atatggactt gttatcaggc tttgggttat
     2341 attagagaag cctatcaaac actagcgcgt aaggaactaa ctatggaagt cttggactgt
     2401 tcggctattt tattgtcttt gtttatgaac caatccaaga cagctagcaa tatcatgttt
     2461 atgcttgatt tggggaatca tttagatcag tggtccttga aaaaaactgc aacagattta
     2521 gaacagagtc ttcttgcaaa agagagcgat gtattcctag tacagggcga tacggttgtt
     2581 agtataaaga gttccgatgt tcaaatagga gatgtcttga tcttatctca aggaaatgaa
     2641 attctgtttg atgggcaagt agtttcaggt ttaggtatgg tcaacgaaag ttccttgaca
     2701 ggagagagtt ttccagttga aaaaagagag tctgatttgg tttgtgcaaa tacagtatta
     2761 gaaactggag agttacgcat tcgtgtaaca gataatcaga tgaacagccg tattttacag
     2821 ctgattgagt tgatgaagaa atctgaagaa aacaagaaaa cgaaacaacg ctatttcatc
     2881 aagatggcgg ataaagtcgt caaatataat ttcttggggg ctgggctgac ttacctattg
     2941 acaggttctt tttctaaggc tatttctttc ctattggtcg atttctcctg cgctttgaaa
     3001 atctctactc ctgtagctta tttgacagtt atcaaggagg ggttgaaccg tgaaatggtg
     3061 attaaggatg gagatgttct ggagaaatat ctggaagttg atactttctt gtttgataag
     3121 acaggaacaa tcacaactag ttatcctata gttgaaaagg tgttaccttt tggagactat
     3181 agtgaggaag atattctcag aatcagtgcc tgtcttgagg aacacattta tcatcctatt
     3241 gctaatgcca tcgtcaagca agctgagata gagggaattg aacatgagga aatgcatggg
     3301 aaactccaat atatcgcaag caaggggatc aaatctcata tagatggcca accagttctt
     3361 attgggaatt atgtcttgat gcaggatgag cagattcata tcagttcaga acaaaatgct
     3421 ttaattgaag agtacaagag tcactacaat ctcttattct tggcttatca aaatgaattg
     3481 attggaatgt tctgcattca tactcctttg agaaaagaag caaaaacagc cttggataaa
     3541 cttaaggcac aggggaaaaa attgattctg gcaacaggag acaccttgat taggacagag
     3601 gaattagtca aagatttgcc ctttgatcag gtctatacag acttgaaacc tgatggaaaa
     3661 tttgagttag tagagaaact gcagaaagca ggtcacacta ttttgatggt tggagatgga
     3721 ttgaatgact cagcggctct aactctatca gatatcggtg tggtgatgaa tgagagtgca
     3781 gatatttcta agcagatgag tgatatctta ttgttagata atcgtttgga tttcttccaa
     3841 gagttggatt cgttatcatc atctttgcaa acactcatca agaaaaatat tcaagatacc
     3901 gttgtcgtaa atagtagttt gattggcttc ggcttattta attggctcag tccttcaaat
     3961 ctctctatct tacataatct aacaaccttg cgcattgtcc tgcgtagcct gtctattaaa
     4021 aatagataga tgagagttat taacagcaaa aaggagttac ctttctcgag gttaactcct
     4081 ttgtttatag ataaatgttg aatagtttag taagtcaata caaagtattt tttcttccca
     4141 cgacggataa cagtcagttc attttctaac ttatcagcgt cactcaagac atagtcaagc
     4201 tcttggatgc ggtcgccgtt tacgtagatg gctccgtttt ggacgtcttc acgggcttgg
     4261 cgttttgagt taactatacc agatgagacg agcagttcca cgatattgtt gttttcgtct
     4321 gcctgtactt ggtagtttgg cacaccacga agtccttgtt tgagctcttt gacagaaagg
     4381 tttttgatgt ttcctgcaaa gagttgctca gtgatgttaa gtgcttcttt gtaggcttct
     4441 tctccgtgaa caagtgtaac aacttcacga gccaagactt tttgagccaa gcgttcgtgt
     4501 ggcgctgctt caaattgttt acgaatatct tcaatctcat caagtgacaa gaaagtaaag
     4561 attttcaaga agcgaacagc gtcagcgtcc atcacgttca tccagaattg gtacatttcg
     4621 tatggagaag tcttttcggg attgagccag acggcatttc cttctgattt accaaatttc
     4681 ttaccagttg catctgtgat tagtggaaca gtgataacgt gaccagtctt gtccgcctta
     4741 cgacgaagca attcggtacc agctgtcata tttccccact ggtcagaacc accgatttga
     4801 agagtgacat tatggtcttg gttaaggacg aagaagtcat acccttgcat gatttggtaa
     4861 gcgaactcag tgtaagaaat tcctgtttcg atccgttttt taacagattc cttactcatc
     4921 atgtagttga ccgtgaagta ttttccaata tcacggagga agtcaatgaa gctgatgctg
     4981 ccaaaccagt cgtagttgtt gaccatgaca gccttgtttt cgccattttc aaagtcaaga
     5041 aaacgagaaa gttgtccttg gatagacttg acccagccat ctactgtgtc ttttgtttgg
     5101 agactacgtt cagcatcttt gaaggacgga tctccgatga gacctgtagc accgccaacg
     5161 agcgcataag gtttgtgacc tgctagttgc aagcgacgac ttgtcaagat tgcgacaagg
     5221 tggcctaggt gaaggctgtc agcagttgga tcgtagccag tataataaga aacttgacct
     5281 tcttctaggg ctttacgcaa agcttcttca tcagtcgttt gaaatatcaa accacgctct
     5341 tttagctcat caaaaatgtg catgtgtttt ttctcctttt taaaattatt gtttctacct
     5401 attgtatcac aaacttgggc aatagcctag cagaataggg aagaaagtgg ctattttatt
     5461 gaaaatttcc cttttgtggt ataatagata aagtgaggaa atccatgcaa aatcaattaa
     5521 atgaattaaa acgaaaaatg ctggaatttt tccagcaaaa acaaaaaaat aaaaaatcag
     5581 ctagacctgg caagaaaggt tcaagtacca aaaaatctaa aaccttagat aagtcagcca
     5641 ttttcccagc tattttactg agtataaaag ccttatttaa cttactcttt gtactcggtt
     5701 ttctaggagg aatgttggga gctgggattg ctttggggta cggagtggcc ttatttgaca
     5761 aggttcgggt gcctcagaca gaagaattgg tgaatcaggt caaggacatc tcttctattt
     5821 cagagattac ctattcggac gggacggtga ttgcttccat agagagtgat ttgttgcgca
     5881 cttctatctc atctgagcaa atttcggaaa atctgaagaa ggctatcatt gcgacagaag
     5941 atgaacactt taaagaacat aagggtgtag tacccaaggc ggtgattcgt gcgaccttgg
     6001 ggaaatttgt aggtttgggt tcctctagtg ggggttcaac cttgacccag caactaatta
     6061 aacagcaggt ggttggggat gcgccgacct tggctcgtaa ggcggcagag attgtggatg
     6121 ctcttgcctt ggaacgcgcc atgaataaag atgagatttt aacgacctat ctcaatgtgg
     6181 ctccctttgg ccgaaataat aagggacaga atattgcagg ggctcggcaa gcagctgagg
     6241 gaattttcgg tgtagatgcc agtcagttga ctgttcctca agcagcattt ttagcaggac
     6301 ttccacagag tcccattact tactctcctt atgaaaatac tggggagttg aagagtgatg
     6361 aagacctaga aattggctta agacgggcta aggcagttct ttacagtatg tatcgtacag
     6421 gtgcattaag caaagacgag tattctcagt acaaggatta tgaccttaaa caggactttt
     6481 taccatcggg cacggttaca ggaatttcac gagactattt atactttaca actttggcag
     6541 aagctcaaga acgtatgtat gactatctag ctcagagaga caatgtctcc gctaaggagt
     6601 tgaaaaatga ggcaactcag aagttttatc gagatttggc agccaaggaa attgaaaatg
     6661 gtggttataa gattactact accatagatc agaaaattca ttctgccatg caaagtgcgg
     6721 ttgctgatta tggctatctt ttagacgatg gaacaggtcg tgtagaagta gggaatgtct
     6781 tgatggacaa ccaaacaggt gctattctag gctttgtagg tggtcgtaat tatcaagaaa
     6841 atcaaaataa tcatgccttt gataccaaac gttcgccagc ttctactacc aagcccttgc
     6901 tggcctacgg tattgctatt gaccagggct tgatgggaag tgaaacgatt ctatctaact
     6961 atccaacaaa ctttgctaat ggcaatccga ttatgtatgc taatagcaag ggaacaggaa
     7021 tgatgacctt gggagaagct ctgaactact catggaatat ccctgcttac tggacctatc
     7081 gtatgctccg tgaaaatggt gttgatgtca agggttatat ggaaaagatg ggttacgaga
     7141 ttcctgagta cggtattgag agcttgccaa tgggtggtgg tattgaagtc acagttgccc
     7201 agcataccaa tggctatcag accttagcta ataatggagt ttatcatcag aagcatgtga
     7261 tttcaaagat tgaagcagca gatggtagag tggtgtatga gtatcaggat aaaccggttc
     7321 aagtctattc aaaagctact gcgacgatta tgcagggatt gctacgagaa gttctatcct
     7381 ctcgtgtgac aacaaccttc aagtctaacc tgacttcttt aaatcctact ctggctaatg
     7441 cagattggat tgggaagact ggtacaacca accaagacga aaatatgtgg ctcatgcttt
     7501 cgacacctag attaacccta ggtggctgga ttgggcatga tgataatcat tcattgtcac
     7561 gtagagcagg ttattctaat aactctaatt acatggctca tctggtaaat gcgattcagc
     7621 aagcttcccc aagcatttgg gggaacgagc gctttgcttt agatcctagt gtagtgaaat
     7681 cggaagtctt gaaatcaaca ggtcaaaaac cagggaaggt ttctgttgaa ggaaaagagg
     7741 tagaggtcac aggttcgact gttaccagct attgggctaa taagtcagga gcgccagcga
     7801 caagttatcg ctttgctatt ggcggaagtg atgcggatta tcagaatgct tggtctagta
     7861 ttgtggggag tctaccaact ccatccagct ccagcagttc aagtagtagt tctagcgata
     7921 gcagtaactc aagtactaca cgaccttctt cttcaagggc gagacgataa ttctctaaat
     7981 gaagtggcca atcaatggat tgccactttt ttcttttcgt gttacaataa gggtatgaat
     8041 cagtatcaga aaaagattgt taacggaaaa atttattcgc tcctatccgg cttaatatgg
     8101 ggaatctgtg gaattttagg agagtacttc tttactcatt atcaggtgtc ttcgggctgg
     8161 attacctcta tgcgtttgac actggcaggg agtcttgtac tcatttggtc tgcaatacaa
     8221 ttaaaatcgc aagtgctaga tatttggcga gacaagaaaa attacctgcc ctttttagcc
     8281 tatgctattt tggggatttt ttcagttcag tattttttct atctctgtgt agaatactca
     8341 aatgctacga cagcaactat tttacagttt attagccctg tctttatcct cttttacaat
     8401 cgcttggttt atcaaaaacg agcgtcaaaa agcgctgttt tctatgtttt ggttgccatg
     8461 ctgggtgttt gcttgatggc gacaaaggga gatctctctc agttatccat gacgccgcta
     8521 gctcttataa caggtttgct gagtgccatg ggtgttatgt ttaatgttat cttgccccaa
     8581 ccttttgcta agcgttatgg ttttgttcct acggttgggt gggggatgat tttggcaggt
     8641 ttgtttagca atgtcctctc gccggtttat cagctttcct ttactcttga tatttggagt
     8701 atcttgattt gcctcattat cgctttcttt ggaacggctt ttgctttttt catttccatg
     8761 aaggctgtgt ccttggtttc tcctttggtg gttgccgtta tcagtgccag tgaacctctc
     8821 tcttctgctc tcttgagtgt tttgttctta ggattagtag tggattggtc cctccttcta
     8881 gctatagcct tgattatttt acccatgata tttttgtcta tagaagaagc gaaagaaagt
     8941 agataaaaag tcttttctta attctaaaag tgtgctatac tagaatagtc aataaaacac
     9001 ggggagatag ttgtgaagag tgtttttagg ttgtatcggt aggggcttgc ttttaccgac
     9061 agcacgtttt atctcacaac cctaaaaaat catacctaaa aacagacaaa aaggtttcaa
     9121 atttgaggcc ttttttggta gaataggtat cattataacg aaccaggagg cacctatgac
     9181 tgctacaaaa atgaacgctc aagaaattat ccaatttatc gccaatgctg aaaagaaaac
     9241 cagtgtcaaa gtaacctttg aggggcaact cgcaactgct gtgcctagct ctgttgtcaa
     9301 actaggaaat gtcctattcg gagactggaa ggacgtggct ccgcttcttg aaggtttggt
     9361 agaaaatcaa gattatgttg tcgagcaaga tgctcgtaat tctgcagttc ctttgctaga
     9421 taagcgtgct atcaacgctc gtatcgagcc aggtgctatt atccgtgacc aggtggaaat
     9481 tggtgacaat gctgttatca tgatgggagc tgttatcaat atcggtgctg aaatcggtgc
     9541 tggaaccatg attgacatgg gtgccatcct tggtggccgt gccatcgttg gaaaaaatag
     9601 ccacgttggt gcaggtgcag ttttggcagg tgtgattgag ccagctagtg ctgaaccagt
     9661 ccgtgtcgga gacaatgttc ttatcggtgc taatgcagtg gttatcgaag gagtccaaat
     9721 cggtagtggt tcagttgtcg ctgcaggagc tatcgttacc caagatgtcc cagaaaacgt
     9781 ggtagtagca ggtgttccag ctcgtatcat caaagaaatt gatgcccaaa cccaacaaaa
     9841 aacagcgcta gaggatgcgc ttcgtacctt gtaattgtaa aagtaaaaaa gaggcggaac
     9901 cctttttcca gcctctttct gctatatcgg aggacagata gatgttagat ttgattcaga
     9961 ctagacgagc tttacaccag attccagaga ttggcttgga ggagttcaag actcaggctt
    10021 atttgctgga tgtgattgag aaattgacta cgggcaagga ttttgttcaa attcgtactt
    10081 ggcggacagg gattttggtc tacctgcagg gaagtcagcc ggagcgaacc attggttggc
    10141 ggacagatat tgatggtctg cctatcgtcg aacaaacagg tctacctttt gcttctcaac
    10201 accaaggtcg catgcatgcc tgtggtcatg attttcatat gaccattgcc ttaggctgtc
    10261 ttgagcgcgc ccttgaggag caacccaaga ataatctgct ctttctattt cagcctgctg
    10321 aagaaaatga agctggtggt atgctcatgt atgaggacgg tgcttttgga gattggttgc
    10381 cagatcaatt ctatggtctc catgttcgtc cagatttgaa agtcggccag attgcgacta
    10441 atactcatac actctttgca ggaacttgcg aggtgaagat ccgtttcaaa ggcaaaggtg
    10501 gccacgcagc ttttcctcat gaagccaatg acgccttggt ggcggctagt tactttgtga
    10561 cccaagtgca gtcagttgtt agccgcaatg tcaacccaat cgagggagca gtggtgacct
    10621 tcggcgtttt tcaagctgga accaccaaca atgtcatcac agacacagct tttttacatg
    10681 gaactattcg tgccttgaca caggacatga gtctcttggt gcaaaagagg gtaaaaacag
    10741 ttgcagaagg ggttgcagct gcctttgata tggaagtcga agtggaactc aaacaaggtg
    10801 gatacctacc tgttgagaac aatccagcct tggcgcgtga actgatggac ttctttgacg
    10861 aaaaagacgg aatcgagttg attgatatcg aacctgctat gacaggcgag gactttggtt
    10921 atctcctttc aaaggttgat ggcgtgatgt tttggctagg tatcgatagt ccctacgctc
    10981 ttcatcaccc tcaaatgagt cctaaggaag aagtcttagc tattggggtg gctgcggtgt
    11041 ctagtttcct gaaaaagaag gcagcagaat agagggcttg tctatgaaat cggaattacg
    11101 caagcaagtc ttgcatgaaa tgaaggcttt atctcaagaa caaaaacagg ctatagacca
    11161 agctttaacc gagcgaattt tacaacaccc cttttatcaa gaagccaagg tcatcgcaac
    11221 ctacctctct ttttctcatg agtttcaaac gcgggaactg attgagcagg cgctgaagga
    11281 cggcaagaag gttttgacac ctaagaccta tcccaagggg cgcatggact ttgtagtcta
    11341 tgatccgcag cagttggtaa aaactgcctt tgccttactg gagccacagg gagatttgga
    11401 agtggtggat gtatctcaga ttgatttgat tcatgttcct ggcctggctt ttacgacgga
    11461 tggatatcgg attggatatg gtggaggtta ttatgaccgc tatctggaac atttttctgg
    11521 tcatactttg agtacggttc atccttgtca aattcaggac tttatacctg aaaaccatga
    11581 tattcctgtt caggaggtat taattgatga aggaaatctt tgatagacgt taccctgtga
    11641 cgagtttctt cctcttagtg acggccttgg tatttttact aatgttggtc actgcaggcg
    11701 gaaactttga cagggcagat acattatttc gatttggagc catgtatggg ccagctattc
    11761 gcctctttcc cgagcaggtt tggcgtctct tgtctgccat ttttgttcat attgggtggg
    11821 aacatttcat tgttaatatg ctttcacttt attatcttgg aaggcaggta gaggagattt
    11881 tcggctctaa gcagttcttt tttctctatc tcttatcagg aatgatgggc aatctctttg
    11941 tttttgtatt tagtcctaaa tccttagcag caggcgcctc tacctctctt tatgggctat
    12001 ttgccgcgat tattgttctt cgctatgcaa ctcgcaatcc ttatatccaa cagctagggc
    12061 aatcctatct gacacttttt gtggttaaca ttattggaag tgttctgatt ccaggaatca
    12121 gcctagcagg ccatatcggt ggtgcagttg gtggcgcatt tctagcagtt atctttccag
    12181 ttagaggaga aaaacggatg tataacacca gccagagatt aggagcggta gtcttgttcg
    12241 taggactcgc cattttgctt ttctacaagg gaatgggaat gtgacgaagt tgaaagaaaa
    12301 tttgtgtaat gaaaaaagat aaggcatttt ttgaacctta tctttttatt ttattccttc
    12361 tcagtcaatt cttttccaag ttggatgagg taattcttca aatcatcttt gacttgtgga
    12421 tgtttgaggg cgtagtcaat agatgttttc ataaagccaa acttatcccc aacgtcgtaa
    12481 cgagctcctg taaattcacg ggcaaaaaca cgctgtgttt tattaagagt atcgattgca
    12541 tcagtaagtt ggatttcatt tccagcacca ggagcttggt tttcgaggat ttggaaaatt
    12601 tcaggcgtga ggaggtagcg tccgataata gcaaggtcgc taggagcgtc ctctggagct
    12661 ggtttttcaa caaaggtttc aacactgtaa agaccatctt ttccttcgcc ttgcggagca
    12721 ataaccccgt aagcagatac ttcgtcatga gggactggca tgacagcgat agtagacgcg
    12781 tgggtacgct cgtagtcatc catgagttgt ttggtaagtg gaacagcctt ttcgtctgtg
    12841 atatccatca agtcatcacc aagcataacg acaaaaggtt catttccgac gaaagccttg
    12901 gcttgcaaaa cagcatctcc gagaccgcgt ggatgagttt ggcggataaa atgcagccgc
    12961 atgtcagttg ttttatcaac tagcttcaaa agatctgttt tccctttttc tttgaggtta
    13021 tattccaatt cgaagtttga atcaaagtgg tcctcaatag aacgttttga tttaccagtg
    13081 acaactagaa tatcttcaat acctgatttg agagcttctt ccacgataaa ctggatagtt
    13141 ggtttgtcta cgattggcaa catttctttg gcaagggcct tggttgctgg taaaaatcga
    13201 gttcctagtc cagcagcagg gatgactgcc tttctaactt ttgatgtcat aaaaatcctt
    13261 tctatagagg gttaagacca ctcattttct gctttaaatt cattgttcat gatgtcataa
    13321 atggcatctt tgatattggt tccgtgataa ataacttggt aaatagcctg tgtaatgggc
    13381 atatagactc caagttcttg ggctagttca taggctgctc gagtcgttga aattccttcg
    13441 attaccatgc ccatattagc ttctatatca gctagggatt ctcctcgtcc gagagcatct
    13501 ccagctctcc agttacgaga gtggatggaa gttcccgtta cgatcaaatc tcccacacca
    13561 gataagccgc tataggtcaa tggactggcc ccgagtgcta cccctaggcg ggtgatttct
    13621 gctaaacctc gagcgatgat ggctgcctta gcattatcac caaatccaag accatgtaaa
    13681 gctccagcac cgacagcaat aatattttta agagcaccag cagtttcaac cccgataaca
    13741 tccgtattgg tataaagtcg gaagtagtga ttactaaata gcttctgaac gtattgagct
    13801 gtttgtaaat ctttagaagc agcagttatt aaagttaggt cacgcacaat ggtctcttct
    13861 gcatgactag gccctgaaac aacgacgata tcactacgga gatgttcagg aatttcttct
    13921 tcaagaatgg ttgataatcg tttatggcta tcaggttcta atccctttga tgcgtgcatg
    13981 atgataacct tatggtccaa ggtttgtgca acttgctggg caacaagtcg tgtcactttt
    14041 gttgggacaa caaacaaaat cgcatccaca tctttcaatg tttctgctaa gtcggtgtag
    14101 gcaatgatat tttcgtctag aacgacatct ttaaagtagt gcttattagt atggtgtgta
    14161 ttaatttcat tgatttgctc gggaagattt ccccaaatac gtacctcgtg tccattgtca
    14221 tttaagactt gtgaaagggc ggttccccaa gaaccaggcc ccaagacggc gacggtttgt
    14281 ttttccatga tttcctcctt gatagagttc tttctcttat tctatcatat tctaggggat
    14341 tttgcacttg cattactggg gtatggtgga aaaatacaca aaaaccgaga cgaagggata
    14401 aactcttagt ctttagttaa agtgagaaga aatctgatag atagtttgat tcagtatatg
    14461 cttataatag aggtaaacaa ctcaggaggt tcttgtaggt tgcgagagtt tggcgaaaaa
    14521 attaaaagat tacgtttggc taaaaaaatc agtcgttcag aattttgtgg tgatgagtct
    14581 gaattaagta tccgtcaatt aattagaatt gaaaatggag aatccagacc aatactaaca
    14641 aagttaaaat atattgctga acgtttggag gttgaagatt acaagttgat gccaagttat
    14701 atagagttgg ataaggaata cctagaattg aagtatttct tgatgaggac tcctacatac
    14761 gaagatgaaa ctatcgccca aaagaaagag agtgtttttg ataagatttt tgaagagtat
    14821 tatgataggc tacctgagga agaaagattt atcatcccaa attattcata tctggcacta
    14881 acgaactaca cagttcaaaa attaccagaa aagctagttg aaatactgtc cttctggtga
    14941 ttttgcttaa tttctgatgt tcgagacatg attgaggaga tttgtcttga ttttttccta
    15001 aaaaagggca gactcctccc ttggtttgat gctcgatttt taaaataagg tgacttattc
    15061 tgttaggcat gttttcccct gccctctagc tctatatgga acagggagta tcagatttat
    15121 ctggcagatt gtatcaaata aggcttgaaa ctgttttgaa taggcatata gacttgagaa
    15181 tttgagaata tgtcgtctag cagtagagac atattttccg gcaatatgaa ggaagagacg
    15241 tcgaaaacgc ttgatagtca aggcctttac ttcatcacca gctagctgct ttaaaaagag
    15301 gtagagatta taagccagac agcccatcat catacgtact tcattcttaa tcatggtcga
    15361 actatctgtc ttatccccaa agaatcctgc tttcctttct ttgataaagt tttcagcatt
    15421 ccctcgttca cgatagaatt gaaacagctc ctcactatct acactgctta aattggtcaa
    15481 atgaactttt ttacaaccat aaaaaagagc acttaatcgt actcttctct tttaaggatt
    15541 agttgaatta attcaaatcc actagttctc ctgtttctag gtagactagg cgttcacaaa
    15601 tgttagcaat gtaatcagcg aagcgttcca gatgccctat gatataaagg tattgagttc
    15661 cattgggaat tgaggtttct tggtctttca taagtccaat gatttccttt gataaggcat
    15721 aataatattg gtcaatctgt tcatcttttt gagcaatact aatagctttt gaggcttggt
    15781 gcaaaggaaa ggcaaccaat aaatcggcta gcatgctgag ggataattta cccatttggt
    15841 gtaactgttc ttcgtcaggg gctagttgat tttcttttag ttgcaaaaca gctttggcaa
    15901 tgcctgccat atggtctccc atacgttcaa ggtctgaaca agaagacatg atgctaatca
    15961 caaatcgaag gtcagacact tgtggctgct gcaaggccaa caaacgggca caggtcaatt
    16021 cgatagcgct ttgaccttgg ttgatagcat gatccttatt gataattagc tctgccatct
    16081 ccttgtcttt ggaggctaag gccagtaagg cttttgaagc tgtttcaagg acaagttgcc
    16141 ctagtcctaa aaaggattgt tctaattcat gcaattctaa gtcaaattga tttctcatag
    16201 gtttttcctt ccttatccga accgtcctga aatatagtct tctgtgcgct gatcttttgg
    16261 attggtaaac acgtcaacgg tatctccaaa ttcgcaaatt tctcctgtta agaaaaaagc
    16321 agttttatct gaaatacgtg aagcttgttg catgttatgg gtaacaatga taatcgtata
    16381 atcctttttt agttgctgaa tgaggtcttc aatttttaaa gtggagatag ggtctaaggc
    16441 tgaagtcggc tcatccatta acagaatatc aggttctact gctaaagctc gcgcaatgca
    16501 aaggcgttgc tgctgaccgc cagataagga catggcactc tttttaagat catctttgac
    16561 ttcttcccaa atggctgccc cttttaaaga tttctccact aaggcatcta attgtttttt
    16621 gtctcgaata ccatgtgtcc ttgggccata agccacgtta tcatagatag acatggcaaa
    16681 gggattaggc tgttgaaaaa ccatccctac acgcttacgt agctgattaa ggttgaattt
    16741 gctactataa atatcttgct catctaagag gacttggcct tcaatatggc aagaaggaac
    16801 caaatcgttc atccggttaa gggtttttag aaaagttgat ttgccacaac cagatgggcc
    16861 tatcaaggca gtaatctgtc tttctggtaa ttgaatcgaa atatttttta aggcttgaaa
    16921 atccccgtaa aataagtcta ggtgtctgac tgaaaatgtt cccatactag gaagctcctt
    16981 tcacaagttt tcgagataat aagcttgata gagtatttat cattaaaaca gtaataatca
    17041 aaatcacacc ggtagcatag gcttcattga catgtagccc ctcacttgac agcatataca
    17101 tatgcagggc tagagaacga cctgaagaca tgagactact tggcgtattg gtagaggtac
    17161 ctaatgtata catgagggca gctgtctcac caacgatacg gccaatagct agtatcactc
    17221 cagctaaaat acctggcatg gcaactggta gaacaattct aaaaacagtc cgtaacttac
    17281 ctgccccaag tccataactt gcttgacgca tgctatcact aacagataaa agggcttctt
    17341 ctgttgagcg aataatgact ggcaacacca tgatgactga ggttaagatt cctgataaca
    17401 gagagtattg aaaacctaag aagactacaa agaagagcat gccaaacaga ccaaaaacaa
    17461 tggaaggaat cccagacaag gtatctgagg ccaatcgcat gattttaaca caaagggaat
    17521 ctttttttgt atattccaca agataaaaac cagcaaaaat ccctatgggc aaggctaaaa
    17581 gaagagcacc aaagaccaga ataacggtgg aaataatcgc tggcataagg gaaatgttct
    17641 cagaagtata agtccaagaa aagagggata gacttagatg aggtaagcct ttgatgagga
    17701 taaaaccaat gattaaaaag agagagccaa aggttaaagc tgaaaaacaa taaacgagaa
    17761 gttttagcag gtatttactc ataagatgat tttcctttca agtaggcaaa gtaggcatta
    17821 atcaagagaa taaggaaaaa gagaactgct gaggttgcaa taagggcttc cctatgctga
    17881 cctgatgcgt aagccatttc cagaacaata ttggttgtta aggttctggt tcctgaaaag
    17941 agtccacttg gaataatcgg ctggttgcct gccaccaaaa taactgccat ggtttcacct
    18001 actgcgcgac cgattcctaa aataactgct gataaaatac cagatctcgc agctggcaag
    18061 atgacactaa aaatactccg ttcatgacta gctcctagag ccaagctacc agaataatac
    18121 gttttgggaa ctgttcggat agcagattct gacaaactga taatggttgg caaaatcatt
    18181 attcctaata gtaacgaagc ggttaggaca ctcatgccat ttcctaaaaa gcttctaatc
    18241 caaggcacca ataattgtag gccgaaaaaa ccataaacaa tagatggaat ggccgccatc
    18301 aagttgatag ctgattttaa gaagccatag acgggctttg gacaataata aaccataaac
    18361 accgatgtca agatgcctgt tggcacccca atcacaatcg ctcctaaggt aattaataag
    18421 gaaccaacga tcattggtaa aataccatag cttgccggaa tgttcgttgg cgaccaatca
    18481 ctgcctaata aaaaacgggc aaagccgtag ttagccatga aaggtaagcc attactaaaa
    18541 ataaagaaac agattagcaa aatagctaca acagctactg ttgcactcat gaaaaaaatt
    18601 gccctaaaaa ctgcttcttt gaaggcttgt tttgtcacat cttgtccttt ctagtgaaga
    18661 aagtaaggga gatacgacac ctccctactt gccttcttta tcttattgta cgatgaaacg
    18721 tctgcatctc tttagagatt tatggagcaa acattttatt taatcttgtc ccaggtggtt
    18781 aatttgccac taaaaacgtc tgcaagttca gccatactga cttggcttgc cttattgtca
    18841 ttattgacca caacagcaat accgtctaaa gcaatagcat catgggtgag actcttacct
    18901 tcttcaggag ttaattccct agaaaccata ccaatatcag cggttttctc cttaacagcg
    18961 gtaatacctg ctgaagaccc attagaggta atatcaatcg taacttctgg attttctttt
    19021 ttataagctt ctgctaattt ttccattaaa gaagatactg aagtggaacc tacaacagac
    19081 aacttgcctg ataagtgttg gcttgtatat tctgtggttt cagttttagc ttcaataaat
    19141 ttattatctg tgaccacttg ttgaccttgt ttggagtgga taaagctgat aaaatcttga
    19201 cctagcttgg aaagattaga agaccaaaca atgttgaagg gacgttgaag agggtattca
    19261 ccatctaaaa ctgtgtctcg actagccttg acaccatcaa tctctaaagc tttgacagat
    19321 ttcgttaaag atcccaagga gatgtagccg atagcattag cattcccttg aactgctgag
    19381 agaacacctt ctgtactatt ttgaatcaca gctgttttgg cagtgttgtc aattttttta
    19441 tcaccgtctt ttttgagaat ccctgtgatt tctgtgaagg caccccgtgt tccagagcca
    19501 ttttctcgtg aaatcacctc aatcgttcct gaagctgact gtttggaagc agctgactga
    19561 ttgccacagg caacaagccc aaatcctgat aagccaatgg ctgcaagagt aagcattttt
    19621 ttgaatttca taataatcac ctttatctct atgtattttt cttgtgtagg cttactacat
    19681 ttatagtcta acaagtcttt gtaaaggttt atccctgatt catgtaaaga ttgtgtaaag
    19741 aatcaaaaaa agccactttt gaaaaatggc tgctcctaaa aatagcttta aaaattatta
    19801 gtcctgtgcg aaagattggt taggaagaaa aatcgtgaag caactgcctc tgccaagctg
    19861 actcgtcacc gtgacttggc cacctaataa ttgactgagt tctttgacaa tggcaaggcc
    19921 aagaccagtg ccaccagttt gtctgcttcg acctttatta actcggtaaa aacgttcaaa
    19981 aatacgatcc tgctctaatt gactaatacc aatccctgta tctgatacag aaatcttaat
    20041 gccttcgttc accttttggg tcttgacctc aatttttccc ccttgttcag tgtaacggat
    20101 ggcattggat aaaagattga gtaagatttg ggaaagtaat tgactatctg atacgagggt
    20161 gacatcatct ggcacctgca cctttagctg taaatccttc ttcttgagct gaggttgcaa
    20221 gctttgagtc aaatcctgta caaattctgc caaagaaagg gtcgtccatt gtataggcat
    20281 ttgttgagcc ttagataagg taagaagatg ctcaacaata tgctcaagac gcaagctttc
    20341 tttgtaaata atgtctagaa agtcatcctt gagcgcttct tcttcagctg acatcccctt
    20401 aatggtttca gcaaagccct taatcgaagt aactggtgtc ctcaattcat gggaggcatt
    20461 tgagacaaag gctaaattta acttttcata agttctaatc gttgttaaat catatagcaa
    20521 gacgagcaca gcttccacag attgggtggg gctaaaaacg ggaactgctg tcacttctaa
    20581 aatcaagtca ccctcatgaa acccacttac ttcttgtttt aaccttgttt tttgatcaaa
    20641 ggcttggtga actaaatttc gaatatccat ccgtttgagg tcatcaagtg aacttatgtc
    20701 gccgtccaca tcgggaaaat aatgaggcag agagcgactg gataataaca tctgaccttg
    20761 agcggaaact aaaaacgtcc ccatggttag gtgcgacaga agaacctcca ttgtttcggc
    20821 taggtccttg tattgctgat cctgttggga gactttggtt tttagggcag acacatactg
    20881 agccaaagac tttaagtctt cttgcccttt ttctaaaaag tattcactac tggtcaagag
    20941 aggttggtgc aaggtctcaa aagcaacttc ccatttccaa aggcaaaaga gccagtagcc
    21001 accttgtccc aaagaaaggg ctagaagaaa gagaccgatg cctttactga tccaagttaa
    21061 tgccatccct gcaatcagaa tgaggctaac acttagattg actagccaaa attgaaggta
    21121 gcgtttcatc tataactcct tgaacttata accataaccc cgaatggttc gaataaattg
    21181 aggggcttta ggattgtctt caattttttc cctcaactta ccaatatgaa cgtccaccaa
    21241 acgtgtttcc tgcccaaagt cataccccca gatacgttcc aaaagacgct ctctagtcag
    21301 tgtcatgttg ggatgtttca taagatagag caagagttca aattcttttg gggtcaaact
    21361 cagtaactta ttcgccttgt agacttcatg acgctcaggg tatactttca aggtcccaaa
    21421 tagccaagaa tcgtcagcga tattatctga atcatctcct tcttgttctc ctttagttcg
    21481 cctgaggaca gccttgacac gcgccagcaa ttctctaggg ctaaaaggtt tggtcaggta
    21541 gtcatcagcc cctaattcca aggccaaaac cttatcaaat tcatcacttt tcgcagaaac
    21601 catcataatt ggagttttga cgcctttggc tctcagccgc ttacaaactt ccatgccatc
    21661 taattgtggt aacatgatat caagcaagat aaaatcaaag ggttctgttt ctgccaaagc
    21721 taaggccttc cgtccatttg ttaccaattg agtagaaaag ccttccttac ttaaatggta
    21781 gtcaagcaat ttcagaatgt gttcttcatc atccactaat aagacttgtt ttgtcatcta
    21841 ttatctccta ttggtaacat tataacacaa ttatcagaaa tcctaacatt gctaaatcag
    21901 attaaatttg cttatcaaga ctagtatctg gtcaaacgct caatcatctc cttgtgctct
    21961 ggataggtcg ccagtagatc taccctttca aataattcaa aatcctcaaa ttcaaaacca
    22021 ggagcaacaa gacaagaaac cagagcatca tccttatcaa ctgttgatcc ccaaatagtg
    22081 cccttaggaa cacagtagtg aagttgttgc cctttggata tgtccaggcc taaagtgact
    22141 gcttcgtagt gaccatctgc tgtaatcatg tgaacagtaa gtggggattc tgcatgaaaa
    22201 taccagattt catctgctgt caatcggtga aaatgtgaag gattcgtttc ttcttaataa
    22261 gaaataaata ctggtataaa gcgcccttcc cttaccagca aggtttatag tgtctgaagc
    22321 tttttttgtt tgtctaaaat agccaccttc aacatgggga gctaactcta gagttcttat
    22381 caagtcttct ttatccgtcg gagccaatgg gttgaagtaa ctcttgttca aaggtggtgt
    22441 tacgatttca agaactcctc tcagctctga ggacacgata atgattgatg cgacggaagt
    22501 aaaaatcaat cgccctaaaa aaacaattag cgaataattc tggtaaaaag aaatttcacg
    22561 ctatgaaggc tcaagcgatt gtcacaagtc aagggagaat tgtttctttg gatatcgctg
    22621 tgaactattg tcatgatatg aagttgttca aaatgagtcg cagaaatatc ggacaagctg
    22681 gtaaaatctt ggctgacagt ggttatcaag ggcccatgaa gatatatcct caagcacaaa
    22741 ctccacgtaa atccagcaaa ctcaagccgc taacagttga agataaagcc tgtaaccatg
    22801 cgctatctaa ggagagaagc aaggttgaga atatttttgc caaagtaaaa acgtttaaaa
    22861 tgttttcaac aacctatcga aatcatcgta aacgcttcgg attacgaatg aatttgattg
    22921 ctggcattat caatcatgaa ctaggattct agttttgcag gaagtctatt atttggttag
    22981 gtgaattagt gaagcgttta ggcaagtgtc tctggttacg acgtcatggc ctctaaatcg
    23041 attatattta ggggtcatga ctagtgaagc agttagctag ttcgcatata agcggctagc
    23101 gtctaacaat taggaacttt agttccaata actttaagat tacgacgttt taggacataa
    23161 atcgatcata tttatgtcct aaaactagtg aagcgcctag ccaaagtccg aataggattt
    23221 ggcgttagtt acttagattg ctttgcaatc aagtaacttt ggcgatttac atcttctctg
    23281 gcgcttctac tccaagcaag cgaagggctt ctttgagaac gactgcggtt gcgtagctga
    23341 gggctagacg gctgtcgcgt tctgggcttt catccaagat acgtgtatgt gcatagtatt
    23401 tgttaaagga ttgagccagg ctaattgcaa atttagcaat gatagaaggt tcaaagttat
    23461 ctgccgcacg gttgataata cgtgggaagt cttgaatgag tttaatgatt tcccagcttt
    23521 cagtatcatt caagctatag ttgccagctg tttctggttt gaaatcggct ttgcgtaaga
    23581 tagattggat acgagcgtag gcatattgaa cgtaaggtcc agtttcaccc tcgaaggata
    23641 ccatagcttc taggtcgaag tcgtatccat ttgtacggtc ggttttgagg tcatagaatt
    23701 taatggctcc aaccccaaca gcatgtgcta cttggtcttt gttttctagt tcaggatttt
    23761 tagcctcgat ttggaccttg gcacggctaa cagcctctgc aatagtaggc tctagcaaga
    23821 tgacattccc tttacgagta gagagcttct tcccttcttt tgtaaccaga ccaaaaggaa
    23881 cgtgggtaat gtcgtcactc caatcgtagc ccatctcttg caagacagct ttgagctgtt
    23941 taaagtgggc agattgttct tgaccaacga cgtagataga tttagcaaat tggtattcat
    24001 ttttacggta gagggctgca gccaagtcac gtgtgatata gagagttgca ccatcagact
    24061 tcttgatgag ggctggatgt tcaattccgt atttctcaag attcacaact tgggcacctt
    24121 ctgattcaag aagtagtcct ttttcagaaa gaatgtctac aactgcatcc atcttatcat
    24181 tgtagaaggc ttctccgtta tagctgtcaa attcaacctt caattcattg taaaggcggt
    24241 taaattccac taaactttca tcgcggaacc attgccaaag agcgagagct tcctcatctc
    24301 cattttcaag tttacggaac cattcgcgcg cttcttcatc caagctaggg tcattttcag
    24361 cttcagcgtt gatgcggaca tagagtttaa ggagttcatc gattggatga gcttttacag
    24421 cttcttcgtc gccccatttt ttgtaggcaa caatcaacat cccaaattgt ttaccccagt
    24481 ctcccaaatg gttgaccttg accgtttgat aaccgatttt ttggaaaata tgtgacaagc
    24541 tatctccgat aacagttgaa cgcaggtggc caatagaaaa tggtttagcg atattcggac
    24601 tagacatgtc gataacaaca ttttcttgtt taccaatatt ttggtcagca tagtgttctt
    24661 tttcagtggt aacagcttgc aatacttgag cagaaatggc agatttatca aggaaaaagt
    24721 taacgtaagg tcctgttgcg acaacttttt caaaggcttg gctgttcatt ttttcagcca
    24781 gttcagccgc aatcatttgt ggtgctttac gttcgacttt tgcaagagaa aaagcaggga
    24841 aagcaatgtc tcccatttct gagtttttag gggtttccag taactttaaa atagcctctt
    24901 ggtccaggct atcaatgatg ctagataatt tgctagcaat caattctttt gtattcatta
    24961 agagctcctt tttggacttt tctactattt tatcacaatt ttaaagaaag aagaaaaaat
    25021 ttttgaaatc tcctgttttt ttggtataat atggttataa atatagttat aaatatgcac
    25081 gcaagaggat tttatgagaa aaagagatcg tcatcagtta ataaaaaaaa tgattactga
    25141 ggagaaatta agtacacaaa aagaaattca agatcggttg gaggcgcaca atgtttgtgt
    25201 gacgcagaca accttgtctc gtgatttgcg cgaaatcggc ttgaccaagg tcaagaaaaa
    25261 tgatatggtg tattatgtac tagtaaatga gacagaaaag attgatttgg tggaattttt
    25321 gtctcatcat ttagaaggtg ttgcaagagc agagtttacc ttggtgcttc ataccaaatt
    25381 gggagaagcc tctgttttgg caaatattgt agatgtaaac aaggatgaat ggattttagg
    25441 aacagttgct ggtgccaata ccttattggt tatttgtcga gatcagcacg ttgccaaact
    25501 catggaagat cgtttgctag atttgatgaa agataagtaa ggtcttggga gttgctctca
    25561 agacttattt ttgaaaagga gagacagaaa atggcgatag aaaagctatc acccggcatg
    25621 caacagtatg tggatattaa aaagcaatat ccagatgctt ttttgctctt tcggatgggt
    25681 gatttttatg aattatttta tgaggatgcg gtcaatgctg cgcagattct ggaaatttcc
    25741 ttaacgagtc gcaacaagaa tgccgacaat ccgatcccta tggcgggtgt tccctatcat
    25801 tctgcccaac agtatatcga tgtcttgatt gagcagggtt ataaggtggc tatcgcagag
    25861 cagatggaag atcctaaaca agcagttggg gttgttaaac gagaggttgt tcaggtcatt
    25921 acgccaggga cagtggtcga tagcagtaag ccggacagtc agaataattt tttggtttcc
    25981 atagaccgcg aaggcaatca atttggccta gcttatatgg atttggtgac gggtgacttt
    26041 tatgtgacag gtcttttgga tttcacgctg gtttgtgggg aaatccgtaa cctcaaggct
    26101 cgagaagtgg tgttgggtta tgacttgtct gaggaagaag aacaaatcct cagccgccag
    26161 atgaatctgg tactctctta tgaaaaagaa agctttgaag accttcattt attggatttg
    26221 cgattggcaa cggtggagca aacggcatct agtaagctgc tccagtatgt tcatcggact
    26281 cagatgaggg aattgaacca cctcaaacct gttatccgct acgaaattaa ggatttcttg
    26341 cagatggatt atgcgaccaa ggctagtctg gatttggttg agaatgctcg ctcaggtaag
    26401 aaacaaggca gtcttttctg gcttttggat gaaaccaaaa cggctatggg gatgcgtctc
    26461 ttgcgttctt ggattcatcg ccccttgatt gataaggaac gaatcgtcca acgtcaagaa
    26521 gtagtgcagg tctttctcga ccatttcttt gagcgtagtg acttgacaga cagtctcaag
    26581 ggtgtttatg acattgagcg cttggctagt cgtgtttctt ttggtaaaac caatccaaag
    26641 gatctcttgc agttggcgac taccttgtct agtgtgccac ggattcgtgc gattttagaa
    26701 gggatggagc aacctactct agcctatctc atcgcacaac tggatgcaat ccctgagttg
    26761 gagagtttga ttagcgcagc gattgctcct gaagctcctc atgtgattac agatggggga
    26821 attatccgga ctggatttga tgagacttta gacaagtatc gttgcgttct cagagaaggg
    26881 actagctgga ttgctgagat tgaggctaag gagcgagaaa actctggtat cagcacgctc
    26941 aagattgact acaataaaaa ggatggctac tattttcatg tgaccaattc gcaactggga
    27001 aatgtgccag cccacttttt ccgcaaggcg acgctgaaaa actcagaacg ctttggaacc
    27061 gaagaattag cccgtatcga gggagatatg cttgaggcgc gtgagaagtc agccaacctc
    27121 gaatacgaaa tatttatgcg cattcgtgaa gaggtcggca agtacatcca gcgtttacaa
    27181 gctctagccc aaggaattgc gacggttgat gtcttacaga gtctggcggt tgtggctgaa
    27241 acccagcatt tgattcgacc tgagtttggt gacgattcac aaattgatat ccggaaaggg
    27301 cgccatgctg tcgttgaaaa ggttatgggg gctcagacct atattccaaa tacgattcag
    27361 atggcagaag ataccagtat tcaactggtt acagggccaa acatgagtgg gaagtctacc
    27421 tatatgcgtc agttagccat gacggcggtt atggcccagc tgggttccta tgttcctgct
    27481 gaaagcgccc atttaccgat ttttgatgcg atttttaccc gtatcggagc agcagatgac
    27541 ttggtttcgg gtcagtcaac ctttatggtg gagatgatgg aggccaataa tgccatttcg
    27601 catgcgacca agaactctct cattctcttt gatgaattgg gacgtggaac tgcaacttat
    27661 gacgggatgg ctcttgctca gtccatcatc gaatatatcc atgagcacat cggagctaag
    27721 accctctttg cgacccacta ccatgagttg actagtctgg agtctagttt acaacacttg
    27781 gtcaatgtcc acgtggcaac tttggagcag gatgggcagg tcaccttcct tcacaagatt
    27841 gaaccgggac cagctgataa atcctacggt atccatgttg ccaagattgc tggcttgcca
    27901 gcagaccttt tagcaagggc ggataagatt ttgactcagc tagagaatca aggaacagag
    27961 agtcctcctc ccatgagaca aactagtgct gtcactgaac agatttcact ctttgatagg
    28021 gcagaagagc atcctatcct agcagaatta gctaaactgg atgtgtataa tatgacacct
    28081 atgcaggtta tgaatgtctt agtagagtta aaacagaaac tataaaacca agactcacta
    28141 gttaatctag ctgtatcaag gagacttctt tgacaattct ccactttttt gctagaataa
    28201 catcacacaa acagaatgaa aaggagctga cgcattgtcg ctcccttttg tctatttttt
    28261 aaggagaaag tatgctgatt cagaaaataa aaacctacaa gtggcaggcc ctggcttcgc
    28321 tcctgatgac aggcttgatg gttgctagtt cacttctgca accgcgttat ctgcaggaag
    28381 tcttaggcgc cctccttgct gggaaatatg aagctattta tagtatcggg gcttggttga
    28441 ttggtgtggc cgtagtcggt ctagttgctg gtggactcaa tgttgtcctc gcagcctata
    28501 ttgcccaagg agtttcatcc gaccttcggg aggatgcctt ccgtaaaatt caaacctttt
    28561 cttatgctga tattgaacaa tttaatgcgg gaaatctagt cgttcgaatg acaaatgata
    28621 tcaaccagat tcagaacgtt gtcatgatga ccttccagat tcttttcaga cttcccctct
    28681 tgttcatcgg ttcgtttatc ctagcggttc aaaccttacc ttctctgtgg tgggtgattg
    28741 ttctcatggt agtcttgatt tttggtttga ctgctgtcat gatgggaatg atggggcctc
    28801 gttttgccaa gtttcaaacc cttcttgagc gcatcaatgc cattgccaag gaaaatttac
    28861 gtggcgttcg tgtggtcaag tcctttgtcc aagaaaaaga gcaatttgct aagtttacag
    28921 aggtctcaga cgagcttctt ggtcaaaacc tttacattgg ttatgccttt tcagtagtgg
    28981 aaccctttat gatgttggtt ggttacgggg cggtcttcct ctctatttgg ctggtcgcgg
    29041 gaatggttca gtcggatccg tctgttgttg gttccatcgc ttcttttgtt aattacctaa
    29101 gccagattat ctttaccatt gttatggttg gatttttggg aaattctgtc agccgtgcca
    29161 tgatttccat gcgtcgtatt cgagaaattc ttgacgcaga gccagctatg accttcaagg
    29221 atatcccaga tgaagagttg gttggaagcc ttagctttga aaatgtgacc tttacctatc
    29281 caatggacaa ggaaccgatg ctgaaagatg tgagctttac tattgaacct ggtcaaatgg
    29341 ttggtgtagt tggagcgact ggtgcaggaa agtcaacctt ggctcaattg attccacgtc
    29401 tctttgatcc acaggaaggg gctatcaaaa tcggaggtaa ggatattcga gaagtgagtg
    29461 aaggaaccct gcgtaaaaca gtttccatcg ttctccaacg tgccattctt tttagtggaa
    29521 cgattgcaga taacttgaga caggggaagg gaaatgctac tctatttgaa atggagcgcg
    29581 cagccaatat tgcccaggct agtgaattca ttcatcgtat ggagaaaacc tttgaaagtc
    29641 cagttgaaga acggggaacc aatttctctg gtggacaaaa acaaaggatg tcgattgcgc
    29701 gtgggattgt cagcaatcca cgtattctga tttttgatga ttcgacctca gccttggatg
    29761 ccaaatcaga gcgcttggtg caagaagctt tgaataagga cttgaagggg acgacaacca
    29821 ttattattgc tcaaaaaatt agctcggttg tccatgcaga caagatcttg gttctaaatc
    29881 aaggacgatt gattggtcaa ggcacgcatg cagacttggt tgccaacaat gccgtttacc
    29941 gtgaaatcta tgaaacacag aaatgaaaga caaactataa gaaaagtcaa tagttttatc
    30001 taaactattt cttatttcaa tttgatgatt tggcgatgat tttagagcac ggcaaaaagc
    30061 tattgaaaaa gtccattttt tcaaaggtaa tcctgtgtta atttcagaaa ttacatcact
    30121 ttttgttcgt caaatggcag ctctttttta ggatataaaa cagggttcgg ataagttttt
    30181 ttgcaaggtg gatgatggct acattgtaat gttttccttg ttctaactta gtcttaagat
    30241 aggccttaaa agcaggtgaa aagcgagggc atgctttggc agcttgtatg agtacctacc
    30301 gcagatgagg ggaaccccgt ttgaccattc ttcctgctaa atcaatctga cctgactgat
    30361 aaatagaaga atccagtcca gcgaaagctt gtaattgagc aggattatca aaggcatgaa
    30421 tatttcgaat ctcggctaaa atgaccgccc ctaaacgatt ctcaatccca gtaaccgtcg
    30481 tgatgaccga gttgaactca gccatcaagt cattgacaca ttttttcgcc ttgtcaatga
    30541 gcctcttgta atgtttgatg ttttcattac aagagataaa acgtttatgc gttatcaaac
    30601 tcattaccaa ttgaaacaaa aagctgtggt tagatccttt cggaaattgt caagcgattg
    30661 gaggaaatga actaatccac agcggcttat tccaagtata ccacttgggc tttggcagta
    30721 gctaactgcg ctaaatataa tataaggagg agtaaaatga agacagttca atttttttgg
    30781 cattatttta aggtctacaa gttctcattt gtagttgtca tcctgatgat tgttctggcg
    30841 acttttgccc aagccctctt tccagtcttt tctggacaag cggtgacgca gctagccaat
    30901 ttagttcaag cttatcaaaa tggcaatcca gaacttgtat ggcaaagcct atcaggaatc
    30961 atggtcaatc ttggcctgct ggttttggtt ctatttatct ctagtgtaat atacatgtgt
    31021 ctcatgacgc gcgtgattgc agaatcgacc aacgagatgc gcaaaggcct ctttggtaag
    31081 cttgctcagt tgacggtttc tttctttgac cgtcgacaag atggcgatat cctgtctcat
    31141 tttaccagtg atttggataa tatcctccaa gcctttaacg aaagcttgat tcaggtcatg
    31201 agcaatattg ttttatacat tggtctgatt cttgtcatgt tttcgagaaa tgtgacgctg
    31261 gctctcatca ccattgccag caccccattg gctttcctta tgctgatttt catcgtgaaa
    31321 atggcacgta aatacaccaa cctccagcag aaagaggtag ggaagctcaa cgcctatatg
    31381 gatgagagca tctcaggcca aaaagccgtg attgtgcaag gaattcaaga ggatatgatg
    31441 gcaggatttc ttgaacaaaa tgagcgcgtg cgcaaggcaa cctttaaagg aagaatgttc
    31501 tcaggaattc ttttccctgt catgaatggg atgagcctga ttaatacagc catcgtcatc
    31561 tttgctggtt cggctgtact tttgaatgat aagtctattg aaacaagtac agccctaggt
    31621 ttgattgtta tgtttgcaca attttcacag cagtactacc agcctattat ccaagttgca
    31681 gcgagttggg gaagccttca gttggccttt actggagctg aacgaattca ggaaatgttt
    31741 gatgcagagg aggaaatccg acctgaaaag gctccaacct tcactaagtt gcaagaaagt
    31801 gttgaaatca gtcatatcga tttttcatac ttgcctgata aacctatttt gaaagatgtc
    31861 agcatttctg cccctaaagg ccagatgaca gcagttgttg ggcctacagg ttcaggaaaa
    31921 acgactatta tgaacctcat caatcgcttt tatgatgttg atgctggtgg tatttatttt
    31981 gatggtaaag acattcgtgg ctatgactta gatagtctta gaagcaaggt gggaattgta
    32041 ttgcaagatt cggtcttgtt tagcggaacg attcgagaca atatccgatt tggtgtgcca
    32101 gatgctaatc aggaaatggt tgaggtagca gcaaaagcaa cccacattca cgactatatc
    32161 gaaagtttgc ctgataagta cgatactctt attgatgatg accagagcat cttttcaaca
    32221 gggcagaagc aattgatttc aatcgctcga accctgatga cagatccaga agttctcatt
    32281 ctcgatgaag caacttcaaa cgtagatacg gtgacggaaa gcaagattca gcatgccatg
    32341 gaggtggttg tagcaggtag aactagtttc gtcattgccc accgcttgaa aaccattctc
    32401 aatgcagatc agattattgt ccttaaagat ggagaagtca ttgaacgtgg taaccaccat
    32461 gaacttttga agctaggtgg cttttattca gaactctatc acaatcaatt tgttttcgaa
    32521 taagaaagaa gttgtcctat gtgggcagct ttttcttgtc cataaaaaat gtttatcaca
    32581 gccttaaaaa aaacatatta gacgaaagtc attttgagtg atatgatagg actatcgtta
    32641 gtattcgaaa ggagaggcat catggctaga acggttgtag gagttgctgc aaatctatgt
    32701 cccgtagacg cagaaggcaa aatcattcat tcatctgtat cttgtagatt cgcagagatc
    32761 attcgtcaag tcggtggtct ccctttagtc attcctgttg gtgatgagtc agttgtacgt
    32821 gattatgtgg aaatgattga caaactcatt ttgacaggag gccaaaatgt tcatcctcag
    32881 ttttatggag agaaaaagac cgtcgaaagc gatgattaca atctggtccg tgacgaattt
    32941 gaattggcac tcttgaagga agcgcttcgt cagaataaac caattatggc aatctgtcgc
    33001 ggtgtccaac ttgtcaatgt tgcctttggt ggaaccctca atcaagaaat cgaaggtcac
    33061 tggcaaggac tacctttcgg gacatctcac tctattgaga cagtagaagg aagcgtggtg
    33121 gctaagctat ttgggaaaga aagtcaggtc aactcagtcc atcgtcaaag cattaaagat
    33181 ttggcaccta atttccgtgt aactgctatt gattcaagag accaaactat tgaagcgatt
    33241 gagtctatcg acgagcaccg cattatcggt ttacagtggc atccagagtt tctggttaat
    33301 gaagaagatg gcaatttaga attatttgag tatttattga atgaactgta acgattggaa
    33361 catctggtcg ttctttcttt tattttttca aaatttttag aatgtggcat tttacgcaaa
    33421 cgtttgaatt ctgataaaaa ttggagaaat tcgacaaaaa aacttgaaaa aaacgaaggt
    33481 aagcgttatg atagaaaaga agaaatattg gaggaagaac atgtcacata ttaaatttga
    33541 ttattcaaaa gttttagaca aatttgttgc accacatgaa gtggaataca tgcaatcaca
    33601 agtaacagca gcagatgaat tgatccgtaa aggaactggt gctggtagcg acttcttggg
    33661 atggttggac cttcctgaaa aatacgaccg cgaagaattt gaccgcatct tgaaagctgc
    33721 tgagcaaatc aagtcagaca gcgatgtttt ggttgtgatc ggtattggtg gatcttacct
    33781 tggtgcaaaa gcagcaatcg acttcttgaa tcaccatttt gctaacttgc aaacaaaaga
    33841 agaacgcaaa gctccacaaa tcctttacgc aggaaactca atctcatcta cttaccttgc
    33901 tgacttggta gagtatgttg cagacaaaga cttctcagta aacgtaattt ctaaatcagg
    33961 tacaacaact gaaccagcga ttgctttccg tgtctttaaa gaactcttgg ttaagaaata
    34021 cggtcaagaa gaagctaaca aacgtatcta tgcaacaact gaccgccaaa agggtgctgt
    34081 taaggttgaa gcagacgcta acggttgggg aacatttgtt gttccagatg atatcggtgg
    34141 acgcttctca gtattgacag ccgttggttt gcttccaatc gcagcatcag gagctgacat
    34201 caaagctctt atggaaggtg cgaatgcagc tcgcaaagac tacacttcag acaaaatctc
    34261 tgaaaacgaa gcttaccaat acgcagtcgt tcgtaacatc ctttatcgta aaggctatgc
    34321 aactgagatc ttggtaaact atgagccatc acttcaatac ttctcagaat ggtggaaaca
    34381 attggctggt gaatcagaag gaaaagacca aaaaggtatc tacccaactt cagccaactt
    34441 ctcaactgac ttgcactcac ttggtcaatt tatccaagaa ggaactcgta tcatgtttga
    34501 aacagttgtc cgtgttgaca aacctcgtaa aaacgtgctt attcctactt tggaagaaga
    34561 ccttgacgga cttggttacc ttcaaggaaa agacgttgac tttgtaaaca aaaaagcaac
    34621 tgacggtgtt cttcttgccc acacagatgg tgatgtacca aacatgtatg tgactcttcc
    34681 agagcaagac gctttcactc ttggttacac tatctacttc ttcgaattgg caattgccct
    34741 ttcaggttac ttgaatgcta tcaacccatt tgaccaacca ggtgttgaag cttataaacg
    34801 taacatgttt gcccttcttg gaaaaccagg atttgaagaa ttgagcaaag aacttaacgc
    34861 acgtctataa tagaagaaaa gagtggtttg cccactcttt ttactctctt tatccataga
    34921 aattggactc agccaagact tgtgatataa tatagaaagc aaaaaggcag acgcctagat
    34981 aataggagaa actatgtcaa aagatatccg cgtacgttac gcaccaagtc caacaggact
    35041 actacacatc ggaaatgccc gtacagcatt gtttaactac ttgtatgcgc gccatcatgg
    35101 tggaacattt ctcatccgta tcgaagatac tgaccgtaaa cgccatgtcg aggatggtga
    35161 acgttcacaa cttgaaaacc ttcgctggtt aggcatggat tgggatgaaa gtccagaatc
    35221 acatgagaat tatcgccagt ctgagcgttt ggacttgtat caaaaataca ttgaccaact
    35281 attagctgaa ggaaaagcct ataaatctta cgttacagaa gaagagttgg cagctgaacg
    35341 tgaacgtcaa gaagtagctg gcgaaacacc acgctacatc aatgaatacc ttggtatgag
    35401 tgaagaagaa aaagcggctt atatcgcaga acgtgaagca gctggtatca taccaacagt
    35461 tcgtttggct gtcaatgagt caggtatcta caagtggcat gatatggtca aaggtgatat
    35521 cgaatttgaa ggtggcaata tcggcggtga ctgggttatc caaaagaaag acggttaccc
    35581 aacttacaac tttgccgttg ttatcgatga ccacgatatg caaatctctc atgttatccg
    35641 tggagatgac catattgcta atacaccaaa acagcttatg gtctatgaag ctcttggttg
    35701 ggaagctcca gagtttggtc acatgacctt gattatcaac tctgagactg gtaagaagtt
    35761 atctaaacgt gatactaata cacttcaatt tatcgaagat taccgaaaga aaggttacct
    35821 tccagaagca gtctttaact ttattgctct tcttggttgg aacccaggtg gtgaagatga
    35881 gattttctct cgtgaagaac tcattaaact ttttgatgaa aaccgcctca gcaagtcacc
    35941 agcagccttt gatcagaaaa aattagactg gatgagcaat gattatatca agaatgcaga
    36001 ccttgaaacc atctttgaaa tggcaaaacc attcttagag gaagcaggcc gtttgactga
    36061 caaggctgaa aaattagttg agctctataa accacaaatg aaatcagtag atgagattat
    36121 cccattgaca gatcttttct tctcagattt cccagaattg acagaagcag agcgcgaagt
    36181 catgacgggt gaaacagttc caacagttct tgaagcattc aaagcaaaac ttgaagcgat
    36241 gacagatgat gaatttgtga cagaaaatat cttcccacaa attaaagcag tccaaaaaga
    36301 aacaggtatt aaagggaaaa atcttttcat gcctattcgt atcgcagttt caggcgaaat
    36361 gcatgggcca gaattaccag atacaatttt cttgcttgga cgtgaaaaat caattcagca
    36421 tatcgaaaac attctaaaag aaatctctaa ataaagagga tacaataatg gacatctggg
    36481 aaaagatgta cgaagaagca cagaaactat acaatccaca tgaagtatca gactttgttt
    36541 atgccaatca tgttgtagct gcagtagaag caggagatgg acaaatattt acaggattct
    36601 gtatggaggg aacctgtggt gttttccatc tctgcgcaga acgggcgacc ctcttcaata
    36661 tgtaccaatt ttcaggacaa actaaggtta agaaagtatt agcctttcga gacaaaccac
    36721 cttatggtgg aagttcagcc atgccttgcg gtgcttgtag agaattcctt ttagagctta
    36781 atgctgaaaa taaagatgca gaattcatga tggactataa tataagaaaa acagttaaag
    36841 tcgcagaact aatcccttat tggtggggag aagaacgtgc ttctaagttt aatgagtgat
    36901 agaagagtca gtataattct ggctcttttt actataagtt gaaa
//
LOCUS       DAVMGZ010000006        35698 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000006 DAVMGZ010000000
VERSION     DAVMGZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35698
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.006"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(123..1757)
                     /locus_tag="VYQ93_000233"
     CDS             complement(123..1757)
                     /locus_tag="VYQ93_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3334989.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLVNLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEIPIYFSSMVAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFVFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIALTVFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSTFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1738..2487)
                     /locus_tag="VYQ93_000234"
     CDS             complement(1738..2487)
                     /locus_tag="VYQ93_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3334990.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPAQSLESIYLSLAGRKEEVADASQGH"
     gene            2666..4120
                     /locus_tag="VYQ93_000235"
     CDS             2666..4120
                     /locus_tag="VYQ93_000235"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV3334991.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4210..6828)
                     /gene="alaS"
                     /locus_tag="VYQ93_000236"
     CDS             complement(4210..6828)
                     /gene="alaS"
                     /locus_tag="VYQ93_000236"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV3334992.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6850..7335)
                     /locus_tag="VYQ93_000237"
     CDS             complement(6850..7335)
                     /locus_tag="VYQ93_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV3334993.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSNFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7610..7849)
                     /locus_tag="VYQ93_000238"
     CDS             complement(7610..7849)
                     /locus_tag="VYQ93_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3334994.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8102..9172)
                     /locus_tag="VYQ93_000239"
     CDS             complement(8102..9172)
                     /locus_tag="VYQ93_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3334995.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9169..9942)
                     /locus_tag="VYQ93_000240"
     CDS             complement(9169..9942)
                     /locus_tag="VYQ93_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3334996.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILTQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9939..10745)
                     /locus_tag="VYQ93_000241"
     CDS             complement(9939..10745)
                     /locus_tag="VYQ93_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3334997.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVALLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10726..11883)
                     /locus_tag="VYQ93_000242"
     CDS             complement(10726..11883)
                     /locus_tag="VYQ93_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3334998.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(11942..12892)
                     /gene="murB"
                     /locus_tag="VYQ93_000243"
     CDS             complement(11942..12892)
                     /gene="murB"
                     /locus_tag="VYQ93_000243"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV3334999.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(13004..13763)
                     /locus_tag="VYQ93_000244"
                     /pseudo
     CDS             complement(13004..13763)
                     /locus_tag="VYQ93_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
     gene            complement(13773..14504)
                     /gene="budA"
                     /locus_tag="VYQ93_000245"
     CDS             complement(13773..14504)
                     /gene="budA"
                     /locus_tag="VYQ93_000245"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV3335000.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14646..15917
                     /locus_tag="VYQ93_000246"
     CDS             14646..15917
                     /locus_tag="VYQ93_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV3335001.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15937..16752)
                     /locus_tag="VYQ93_000247"
     CDS             complement(15937..16752)
                     /locus_tag="VYQ93_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV3335002.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQVGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(16909..17562)
                     /gene="phoU"
                     /locus_tag="VYQ93_000248"
     CDS             complement(16909..17562)
                     /gene="phoU"
                     /locus_tag="VYQ93_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV3335003.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17574..18332)
                     /gene="pstB"
                     /locus_tag="VYQ93_000249"
     CDS             complement(17574..18332)
                     /gene="pstB"
                     /locus_tag="VYQ93_000249"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV3335004.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18345..19148)
                     /gene="pstB"
                     /locus_tag="VYQ93_000250"
     CDS             complement(18345..19148)
                     /gene="pstB"
                     /locus_tag="VYQ93_000250"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV3335005.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19159..20043)
                     /gene="pstA"
                     /locus_tag="VYQ93_000251"
     CDS             complement(19159..20043)
                     /gene="pstA"
                     /locus_tag="VYQ93_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV3335006.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20033..20950)
                     /gene="pstC"
                     /locus_tag="VYQ93_000252"
     CDS             complement(20033..20950)
                     /gene="pstC"
                     /locus_tag="VYQ93_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV3335007.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(20950..21828)
                     /gene="pstS"
                     /locus_tag="VYQ93_000253"
     CDS             complement(20950..21828)
                     /gene="pstS"
                     /locus_tag="VYQ93_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV3335008.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22402..23706)
                     /locus_tag="VYQ93_000254"
     CDS             complement(22402..23706)
                     /locus_tag="VYQ93_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV3335009.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKSSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23707..24480)
                     /locus_tag="VYQ93_000255"
     CDS             complement(23707..24480)
                     /locus_tag="VYQ93_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV3335010.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24470..24748)
                     /locus_tag="VYQ93_000256"
     CDS             complement(24470..24748)
                     /locus_tag="VYQ93_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV3335011.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24748..25149)
                     /locus_tag="VYQ93_000257"
     CDS             complement(24748..25149)
                     /locus_tag="VYQ93_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV3335012.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25359..25976)
                     /locus_tag="VYQ93_000258"
     CDS             complement(25359..25976)
                     /locus_tag="VYQ93_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV3335013.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(25966..26739)
                     /locus_tag="VYQ93_000259"
     CDS             complement(25966..26739)
                     /locus_tag="VYQ93_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV3335014.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26750..27487)
                     /locus_tag="VYQ93_000260"
     CDS             complement(26750..27487)
                     /locus_tag="VYQ93_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV3335015.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(27492..28622)
                     /locus_tag="VYQ93_000261"
     CDS             complement(27492..28622)
                     /locus_tag="VYQ93_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV3335016.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28706..29095)
                     /locus_tag="VYQ93_000262"
     CDS             complement(28706..29095)
                     /locus_tag="VYQ93_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV3335017.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29111..29494)
                     /locus_tag="VYQ93_000263"
     CDS             complement(29111..29494)
                     /locus_tag="VYQ93_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV3335018.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29495..30283)
                     /locus_tag="VYQ93_000264"
     CDS             complement(29495..30283)
                     /locus_tag="VYQ93_000264"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV3335019.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFSLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEK"
     gene            complement(30276..31211)
                     /locus_tag="VYQ93_000265"
     CDS             complement(30276..31211)
                     /locus_tag="VYQ93_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV3335020.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31341..31517)
                     /locus_tag="VYQ93_000266"
     CDS             complement(31341..31517)
                     /locus_tag="VYQ93_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV3335021.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31657..32364)
                     /locus_tag="VYQ93_000267"
     CDS             complement(31657..32364)
                     /locus_tag="VYQ93_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV3335022.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            32515..33543
                     /gene="queA"
                     /locus_tag="VYQ93_000268"
     CDS             32515..33543
                     /gene="queA"
                     /locus_tag="VYQ93_000268"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV3335023.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(34204..34593)
                     /locus_tag="VYQ93_000269"
     CDS             complement(34204..34593)
                     /locus_tag="VYQ93_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV3335024.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(34647..35338)
                     /locus_tag="VYQ93_000270"
                     /pseudo
     CDS             complement(34647..35338)
                     /locus_tag="VYQ93_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
BASE COUNT        10658 a         8138 c         6743 g        10159 t
ORIGIN      
        1 cttattcaaa acattcctcc ctatatattt ttgattacca atcttaatca tttacaacta
       61 cattctaaca aactataaaa gcgtttgtcg aattgaattt atcaagcaag cgaccaacca
      121 gctcatcttt tttctatttc tgccaatatg cgtgacaggt aataatgata gccaaaaata
      181 gcaagagcaa gcaggaggat aagagctcct actcccaagt tgatggcaag gataggggag
      241 agagactgaa ccaagaatat gctcccaatt acaagggcca tcaggattgc actataaata
      301 aacaataaaa ctatggcgac tatgccattt gaacgattca ccaggtccgt aatgctactc
      361 caattggttg acagattttt aacgtcctta aagtaatggt ggcaagaaag gatgacactg
      421 gcaatgatcc agactacaag aaggtaaatc atcgaaagga tggacaagcc tagatataga
      481 gaaagaccga gcaaagtcag aactggtaaa aaggactgaa cagcaaagat aatccaaaat
      541 ttcactttca cataacgagc aaagtcaaag ggtaaactct tcagaaaatc aacattttcc
      601 ctctccaagg acaaggcaat tgaatgcagg ctggtgatat tgttattgac aactgctata
      661 aacactccta caaaaaacaa gggtaaccag tatggaggat gaatgtctgg aactatctga
      721 gaatctcgga ttttggaaat cagactgatc atcatgagat aaggaaggaa agtacttgta
      781 aaaagcactg taatcacgcc agtcccctgt cccaagaggg tgaggtggta gcgtaaaacc
      841 atgcggaaaa atcccttttt agtagttgaa atcctctcct tgctgcgacg ttcttttttg
      901 accttctcct cactgttaag caggatcacg tcataaaaac gaggaaggac cttctttttg
      961 gtcaggtaaa gcaagaaaac agttaaagct atccaagcga gcagacccac taaggcttct
     1021 gtcgaaaaag gctccactgc tattttgtaa aagatatgaa gaggataaag gagaaatgga
     1081 atgtctctaa ctttgtcaac aatacttcca aaagtcgact gtagaaagaa gacaaatatt
     1141 aaaggtatga gaactcctat cccaatcatc acattcgaaa aaatagactg atactttctg
     1201 aagaccctag tctgagccaa gaaatgtact gccactaccg tcactaaagt aacagagaca
     1261 aataataagg tcaaggacag tagcatcaaa ggcaaaccca gccaaagaga aggagctaga
     1321 ctaatataga gggctagaaa ataagctagg attggtacaa ttccagttag agctggcaaa
     1381 aggacagaca gtcctttagc aattataatc tctgattctt taaaggcata gggcctatac
     1441 gataccaaat ccttactctc ataaaagaca ttgtaaaagg ccgttaaaga agttgaaaag
     1501 gcaatcacta gtaaaatagc aaccatcgag ctaaaataaa taggtatttc ctcaaaagga
     1561 aaatgaatgg ctatattact aaaacagatg agcatcaaga gactggaaaa aatgtaagaa
     1621 cttaggactc tagcggaaac atttactttt ttcccaggat tcttagcctg cttctttcgt
     1681 agattaacca gattagcttc ttgagatgaa taaaggatat tgatatcaac taatttttta
     1741 atgaccttga gacgcatccg caacctcctc ttttctacca gcaagactaa ggtagatact
     1801 ttccaaagac tgggctgggt aatcttttct caagtcctct acactaccac aataaatcaa
     1861 atgccccttt ttcaaaatgg caatccgatc acagacttgc tctgccacct ctaggacatg
     1921 agttgaaaac aagactgtct tccctttttg tgcatgttcc ttcatcatct gtttcaaatc
     1981 aaaggcagcc tggggatcca aaccagtcaa gggttcatcc aagacccaaa tatcgggatc
     2041 agacaagagt gctccgatga caaagacttt ctgacgcatt ccgtgagaaa gagtttcaat
     2101 aacctgatag cgattttcag caaaatcaaa aacgttcaat agcctagcta gactagtctc
     2161 caagtcagag ctagtcagat cataggatga ggcgatcaat tcccaaaatt cattggccgt
     2221 taaacgtaaa aataagtcag gcgagtctgc tacgtagcca atctttcgtt tgatcgccaa
     2281 gcgattttcc gataactcct gaccatctac caaaatacga ccactgctgg gtgaaatgat
     2341 actgactagg gattttatgg tggtcgattt tccagcccca ttatgaccaa tcaagcccat
     2401 aatctctcca ttttcaatct gcaaattgag attactcaag gcctctttat caccatacaa
     2461 cttactaaca ttttgaaatt caatcatggg atgactccta tctttatctg tattatacat
     2521 cgctatacta gcactttgat acaaaaaaat caatacttta ttttaagtag ttaaaatagt
     2581 ttccttctat cttacgcaaa cgtttgctcc gagagatact ttatgataga ataaagaaca
     2641 agattgacaa gtaagaggaa acatcatgca aaatcaaaca ctcatgcaat actttgaatg
     2701 gtatctgccc cacgacggtc aacactggac gcgtctggct gaaaatgctc cacacctagc
     2761 tcatctgggg atcagtcacg tctggatgcc accagccttc aaggcaacca acgaaaaaga
     2821 tgtcggctat ggggtctatg acttatttga cttaggagag ttcaaccaaa aagggactgt
     2881 ccgcaccaag tatggtttca aagaagacta tcttcaagcc attcaagccc ttaaagcgca
     2941 gggaattcaa cctatggccg atgtagttct caaccacaag gctgctgctg atcacaggga
     3001 agcctttcag gttattgaag tggatcctgt agaccgtaca gttgaacttg gagaaccctt
     3061 caccatcaat ggctggacta gttttacctt cgatggtcgc caagatacct acaatgactt
     3121 ccactggcac tggtaccact tcacaggtac agactatgat gccaaacgcc gtaaatccgg
     3181 gatttaccta atccaaggag acaacaaggg ctgggctaac gaggaattgg tcgataacga
     3241 aaacggaaac tacgactacc tcatgtatgc cgacctagac tttaaacatc ctgaagtcat
     3301 ccaaaacatc tatgactggg ctgattggtt catggaaacg actggtgtag ctggtttccg
     3361 tttggatgcc gttaagcata ttgactcttt ctttatgcgc aatttcatcc gcgatatgaa
     3421 ggaaaaatac ggtgacgatt tctatgtttt tggtgaattt tggaacccag acaaggaagc
     3481 caatctggac tatctcgaaa aaacggaaga acactttgac cttgtcgatg ttcgtctcca
     3541 ccagaatctc tttgaagcca gtcaagctgg cgcaaactat gaccttcgtg gcattttcac
     3601 agatagcctg gttgaactca agcctgacaa ggctgtgact tttgtcgaca accacgatac
     3661 ccaacgagga caagcccttg agtctaccgt tgaagaatgg ttcaagccag cagcctatgc
     3721 cctcattttg ttacgccaag acggccttcc atgtgtcttt tacggagact actatgggat
     3781 ttcaggccag tatgctcaag aagatttcaa agaaatcctt gaccgcctcc tagccatccg
     3841 aaaagacttg gcctatggag aacaaaatga ctactttgac catgctaact gtatcggttg
     3901 ggtacgttca ggtgctgaaa atcaatcccc aatcgcagtc cttatctcaa atgaccaaga
     3961 aaacagcaag tcaatgtttg tcggtcaaga atggactaat caaacctttg tagatttact
     4021 tggtaaccac caaggtcaag ttacaattga tgaggaaggt tatggacaat tccctgtctc
     4081 agctagatcc gtaagtgtct gggcagtcaa taccatctaa tagctcataa taaccaagct
     4141 aggtccaagc ggatttggct tttttgtatt cacaaaaaga cctacccaaa tggatagatc
     4201 tttacttgat tacaatttac ctgctactgc atccaacaat tcttggatct tagcttggtt
     4261 gcttcctcct gccatggcca tgtctggttt accaccacca cgtccatcga tgattggtgc
     4321 taattctttg acaaggtttc ctgcatgaag gtcttttgtc ttgcttgcta caaggacatt
     4381 gactttgtca ccgatagcgg caactaggac aagaagatca gagtagtctt tttgtttcca
     4441 gttatctgca aaagtacgaa gggcaccggc atcggataca gacacttgac tagcaatgta
     4501 acgatgaccg ttgacttcct taacatcttt gaagatatcg cctgcggctg cagctgcggc
     4561 tttttctttc aactcagcat tttctttttg aagttgacga agttgttctt gaagtccttc
     4621 taccttgtga ggtacttcct tgacttgagg tgctttcaag gttgctgcga cagctttaag
     4681 agcatcctct tgttcacgat aggcttcaaa ggcttcctta ccagtcactg ccaagatacg
     4741 gcgagttcct gaaccgattc cttcttcttt gacaattttg aagagaccaa tctcagaagt
     4801 gttgccaaca tgagtaccac cacaaagttc aatagagtag tcaccgatag tcacgacacg
     4861 aacttccttg ccgtatttct caccaaagag ggccatagct cccatttctt tagcagtgtc
     4921 aatatccgtt tcaactgtct tcacttcaag tgcttcccaa attttctcgt taacttgctg
     4981 ttcaatcgca cgaagttcct cagcagttac tgcttggaag tgggtaaagt caaagcgaag
     5041 gaattcaact tcgttaagag atcctgcctg tgttgcgtgg tttccaagga tattgtgaag
     5101 ggcagcgtga agcaaatgag tcgcagtgtg gtttttcatg acacggtgac ggcgattgct
     5161 atcaattgcc aaggtatatt cttggttcaa ggcaagcggt gcaaggactt caactgtatg
     5221 aagggcttga ccatttgggg ctttctgaac attggtcaca gtagccacaa ccttacctga
     5281 ctcatccaag atttgtccgt agtcagctac ctgtccaccc atttcagcat aaaatgacgt
     5341 ttccgcaaag ataagagagg cagttccttc tgaaacagct cctacttctg cattgtcagc
     5401 aacgatagct accaatttag aagacaattg gctagcattg tagttgaaga cactttctac
     5461 agtgatgttt tgaagagttt cattttgcat acccattgag ccacccttga cagctgacgc
     5521 acgcgcgcgt tcttgctgtt ctttcatggc tgcttcaaaa ccttcacggt ctacagtcat
     5581 accagcttct tcagcaattt cttcagtcaa ttcaactggg aatccgtatg tatcatagag
     5641 tttaaagaca tcttgaccag caatgacaga ttgacctttt tctttcaagt ctgccacaat
     5701 gccttgggca aagtgttgac ctgagtgaag ggtacgggca aatgattctt cttcgctctt
     5761 aacgattttc tcaataaagt cacgtttctc aagcacttct gggtagtagc tttccatgat
     5821 ttttccaaca gttggaacaa gtttgtaaag gaaaggctcg ttgataccca atttttgacc
     5881 atgcatagaa gcacgacgga gaagacgacg aagaacataa ccacgacctt catttcctgg
     5941 aagggcacca tcaccgatag caaatgaaag agaacgaatg tggtctgcga taaccttgaa
     6001 gctcatgttg tcgccatctt ggtcataaac cttaccagac aatttctcga cttcacggat
     6061 aatcggcata aagaggtccg tttcaaagtt ggtcttagcc ccttggataa cagccaccaa
     6121 acgctccaaa ccagcgcccg tatcaatgtt cttatgtggc aattccttgt attcgctacg
     6181 aggaacagca gggtctgcgt taaattgtga caaaacgatg ttccagattt caatataacg
     6241 gtcgttttca atatcttctg caagcaggcg aagaccgata ttttctgggt caaaggcttc
     6301 cccacggtca aagaagattt ctgtatctgg tccagaaggt cccgcaccga tttcccagaa
     6361 gttgtcctca attggaatca agtgacttgg atccactccc acttcaatcc agcggttgta
     6421 agaatcttta tcgtctggat agtaggtcat gtaaagtttt tcagcaggga aatcaaacca
     6481 ttcagggctt gtcaaaagct cataagccca agtgatagct tcgtcacgga agtaatcccc
     6541 gatagagaag ttccccaaca tttcaaacat ggtatggtga cgcgcagtct tccctacgtt
     6601 ttcgatgtcg ttggtacgga tagccttttg ggcattggta atacgtggat tttcagggat
     6661 aatggtcccg tcaaagtatt tcttaagggt tgctacccca gagttgatcc acaaaagagt
     6721 tgggtcattt acaggaacca aacttactga tggttctact gagtgacctt tggtcgccca
     6781 gaaatcaagc cacatttggc gtacttgtgc actagatagt tgtttcatat tgtctcctta
     6841 ttcacttgtt taatgtgatt ggctttccag catttccaca tagtcaatcg cgacacagag
     6901 ggaaatgact aggtctgcat aagcgtcttc aagaaccgtt acggtatagg tagaggtcag
     6961 atggaagagt tccttcttaa tttccgcaat caactgatcg cgatcatcca gcaatttgaa
     7021 attcaaatcc cagatattgc cctcgatacg aagacctaga ttatcaaact catacttatc
     7081 tcgccagaag gtcaacttct tacgaatgac aaaattcgag ccatcccgaa gctgaatctc
     7141 aaaacgagga agcaaggtca agatttcttt actgatctga ctgacttgtt caccagccgc
     7201 atcatagatg gtaaaagttt tgggaatctt aaaaaatgat ccctccacct gataggcaat
     7261 ttctcccctg tcatccttga tagcgaagcg ttcgcctcca agacgaaact tttgtttgac
     7321 aagaaatgtt ttcatcaaca cctccaaaaa tcaaaagaca agctcatatc acgaagggcg
     7381 aaaaaccgcg gtaccacctt cattcaatga acttgtcatt ctcttgttct tatgcaattg
     7441 tatgattgag tagcatgact tcctagctta gatggctcgc agcaccgcca tttctctgga
     7501 ctaagacaag tgaaaatcaa ttctcaactt tcttattata acgttttttt aagcttgcgt
     7561 caactggaaa tgatctccgt tgaattagac caattcccta catctctgat tactttttca
     7621 ggatatattt tttcttactg ccatttttct ttttatccca aattttcata ttactaaaca
     7681 cagctactag aatatttcca aatataaagg tgcctatcac ccaatatatg gactcagttg
     7741 ttaggtattg tcgatccaag ccatccttta aatggaatag tatagcagtt tggttaacaa
     7801 tcataaaggt tggccagaaa ctttttttga aaaaagtaga cattttcatt atttgttgcc
     7861 gctttctgta aggttaatac tcaataaaaa tcaaaaagca aactaggaag ctagcctcaa
     7921 gctgtacttg agtacggcaa ggcaacgctg acgtggtttg aagagtatag gcttagtata
     7981 ctactaggca agcaaataaa caaataaaca actagaatag aaaaagatag ggctctaaaa
     8041 actgacttct attccttaaa aacgaaccag cttgactgat tcgtcttctt acgtttatct
     8101 cctacttccg atacatttta aactgtagga agaggtcgct atatttccct gtccatttat
     8161 ggtcaaattt ctcataaact tctaggtgtt tcatggtttc aacatcggga tagaaggcct
     8221 tatcttcctt tgtttcctct gggagcaatt ccttcgctgg taggtttggt gttgaatagc
     8281 cgacatactc cgcattttgg agagcatttt caggtttcaa cataaagttg ataaaggcat
     8341 aggctgagtt ttggttttta actgttttgg gaatgaccat attgtcaaac caaagattgc
     8401 tggcctctgt cggtaccaca taacgtagat tttcattttt ttctaacatt tggctggctt
     8461 caccagagaa ggtcacgccg attgcaacat tattctgaat catatagccc ttcatctcgt
     8521 ccgcaacgat agccttgata tttggagtca gtttgtagag cttatccact gtctcttcca
     8581 actgctgcag atccttggag ttgaggctgt agccgaggga attgagtcct agtcccagca
     8641 cctcacgcgc cccatcaaag agcatgatag aattcttata ctccggcttc caaaggtcat
     8701 cccaatgctc aggcgcttca tctaccatgg tttcgttgta gacaattcct aaggttcccc
     8761 agaagtaagg gatggagaat ttattacctg ggtcaaagga ctggttgaga aactctggtc
     8821 cgatattttc gattccttca atttttgaat aatcaagcgg aaccaagagg tcttcgtcct
     8881 tcatcttgtt aatcatgtat tcacttggaa tggcaatatc gtaggtcgtt ccaccctgct
     8941 ttatcttagt gtacatggct tcgttggagt caaaagtctc gtactgaact tgaattcctg
     9001 tttcttctgt aaactgagtc aagagttcag gatcgatata gtctccccag ttatagataa
     9061 ccaatttttg actatctcga ctattgattt tactatctaa atgagtcgca attccccaca
     9121 agacaaggat aatcgctgca attcctgcta aaaatgaata gatttttttc atgcttgctc
     9181 ctccttctca cgagagataa agtaataacc tacaactagg ataatactaa agagaaagac
     9241 tagagcagac agggcattga tttctaagga aatccccttg cgagcacgag agtaaatctc
     9301 gactgatagg gttgaaaagc catttcctgt tacaaagaag gtcacggcaa agtcatctaa
     9361 cgaataggtg aaggccatga aataaccagt aatgatagac ggagtcaggt aaggaagcat
     9421 gatttccttg aacatctgaa attgactagc tcccaagtca taggccgcat gaatcatgtc
     9481 gccattcatt tccttgagtc gaggcaagac catcaagacc acgataggaa tggagaaggc
     9541 cacgtgacta gatagaacgg tcaaaaagcc aagtgaaaac ttgagttggg taaagagaat
     9601 caagaagcta gcaccaatca taacgtcagg cgcaaccatg aggatattat tgagtgatag
     9661 aaaggcttct tggtatttct tacgagactg gtagatgtaa atggcaccaa aagtcccgat
     9721 aatggtcgct atcaaggctg ataggaaggc caagaaaaag gtctgagtca aaatcagcat
     9781 gagtctccca tctccaaaca tggtttcaaa gtgagtccag ctaaaacctg taaagctatt
     9841 catatcatca ccagcattaa aggcatagcc aatcaagtaa aagataggca ggtagaggac
     9901 cagaaagacc agtcccagat aaaggttggc aaattttttc atcgttctct cctttcctta
     9961 gtcacccaca tggtgatgaa catggtcagg atgagaatca caccgatggt tgaacccata
    10021 ccatagttgt cattggttag aaaattctgc tcaatagccg tccccaaggt gataacgcgg
    10081 ttcccaccaa tcaaacgggt cagcatgaag agactcaaac ttgggataaa gaccgactga
    10141 accccacttc gcacaccgtt catagataga gggaagatga catgacggaa ggtctcccac
    10201 ttagttgcac caaggtcata actagcattg atgagattat tatccatatc gtctaagaca
    10261 ttgaaaatcg gcaaaatcat aaaggggagc tcgatgtagc ttgcgacaaa gataaaggaa
    10321 aaatcggtaa aaagcaactg ttgtgaacca attccgataa attccaagaa ttggttaata
    10381 gagccatttt gaccaaaaat cccgataaaa gcataggctt taaggagcaa attgatccag
    10441 gtaggaagga taatcagcat gagccagagt tgacggtgtt tgagacgggt caaaaagagg
    10501 gccgtcggat aactgataag cagtgccaca aaggtcacaa tgcctgcata aagcactgag
    10561 ttgaaactca ttttaagata ggtcaagttt tgtgacgcaa agtaagattt gtaattttct
    10621 aaactgaact ggccttcgat gttgaaaaag gattgaccga aaatcaagac caagggtgcc
    10681 aatacaaaga gcgcaatcca aagcatgtag ggtactacaa agagtttaga gcttgttttc
    10741 ttcatctctt tcctcctcga ttgcattgat caaacctgct tcttgctctt cgatttctac
    10801 gtactcctca atacgagcat cgaactcttc ttcggtttca ttgagacgca tgatgtggat
    10861 gtcttctggt tcaaagtcca gaccgatttc ctcacccacg atagccttac gggttgagtg
    10921 gatcatccat tcatttccaa gttcgtcata ggcgataatt tcataatgaa ctccacggaa
    10981 aagctgggta tcgaccttaa cttggagctt gccttcttca ggaagggtaa tgcgcaagtc
    11041 ctctggacga ataacgacct caacaggttc atttggcttc atcccaccat caaccgcttc
    11101 aaagcgtttg ccgttaaatt cgaccaagta gtcctcaatc atggtacctg gcaagatgtt
    11161 tgactccccg ataaaggtgg caacaaagtg gttgattggc tcatcgtaga tgtccacagg
    11221 ggttccagac tggacaatct cgccatcatt cataacgaaa atccagtcac tcatggcaag
    11281 agcttcttcc tgatcgtgag tgacaaagac aaaggtaatg cccaatcgtt gttgtaattc
    11341 acgcaattcg tactgcatgt ctgttctcaa tttcaagtcc agcgctgata aaggctcgtc
    11401 caacaagacc acacggggtt ggttgatgat agcacgggcg atggccacac gctgacgttg
    11461 tcctccagaa agtttgcgga tggaacgttt ttcataacct tccaactgaa ccatcttgag
    11521 aacttccgct acacgctgct cgatttcttt cttatcaatt ttacgcaagc gaagtggaaa
    11581 ggcaacattt tcaaacacat tcatatgtgg gaacaaggca taggattgga agacggtatg
    11641 tacgtcgcgc ttgttggttg gaatatcatt gatacgaaca ccgtctagca tgatatctcc
    11701 tgtcgtcgca tccagtaaac ctgcaataat gtttaggata gttgatttcc ccgaaccaga
    11761 tgcacctaga agggtgtaga atttcccttc ttccaactca aagttgatgt ctttgagaac
    11821 cttggtgttg ctgtcttcaa aaactttaga gacgtttttg aattcgataa ttggtttttt
    11881 caattggcat aaattccttc tttttcatag attaaccgat cggggctctg tcaggtcccc
    11941 actacctctt gcagggagta aaaccacctg catacatctt cgctaccgat aggctttcac
    12001 ccaagatccg gacttctctt tcaagcgtaa tacctgagtg ttccttgact ttttcgataa
    12061 ccgattggat caagtcctcg tagtctttgg ccgttccatc tgcgacattg atcataaatc
    12121 ctgcatgctt ttctgacact tctacgccac cgatacgata gcctttcaag ccagcttctg
    12181 aaattaactg acctgcaaaa tgcccgactg gacgcttaaa gaccgagcca caagatgggt
    12241 attccaaagg ttgcttgagt tcacgtaggt gcgtcaagcg gtccatttcc tgcttgataa
    12301 cctgatgggt tcctggagct agggcaaatt taactgacaa gacaactgca ccagactcct
    12361 gaatagctga atggcggtaa ccaaaagcca agtctttagc agacagggtt tcgatttctc
    12421 catccttggt caagacctta caagactgca agatgtgagc aatctcgcca ccataggcac
    12481 ccgcattcat aaagacagca ccgccaacgc ttcctggaat accacaagca aactcaaagc
    12541 cagttaaact atgacggagg gcaatgcgag ttgtttcaat caagttagcc ccagcttctg
    12601 cttcaatggt atagccatca acagaaacgt tattgagctt gtcacacaag atgacaaatc
    12661 cacgaatccc accatcacga acgatgatat tgcttgcatt gccaagaacc atccaaggga
    12721 tattttcttg gttggcaaat ttcacaacgc gagccaactc aaaacgattt cgtggaaaga
    12781 ccaaataatc agcctctcca cctacttttg tataactata gctatgcaag ggttccttaa
    12841 aacggatatc aattccttct aagatttcaa gcattttttc tcttacagac atgtcactct
    12901 tccttttaca aaattcattc cattatacca tttttagaga catttgacga ccataaaaat
    12961 accttgtttg gattttgcat aagaaaaaga ggttcccccc tttttatgat tttttacaaa
    13021 agatttcctt ggttccatag gcgaccagaa cgagctccag tgctagaatc acttcaacca
    13081 agactggatt tgtcaaccag cctacttgga aaagagatgg tgccagatca aagaaggcat
    13141 gcaagccata ggctgctagg agataaatcc atttcttctg gcgaacagct tggtaaaccc
    13201 aaactgtcaa aagtaattgg aaaccaagcg ccaagattcg ctcaaaacca agcaaataaa
    13261 tctgccagac cgaaagtgac tgaatggttt ttaacatatt ttcagacagt aattgcataa
    13321 cctgtggatt ctgagtttga actgccgaaa gaacgatgta aagattgagt aaactagtaa
    13381 ggcctagaaa aatcaactcc aagccaccat gccctaggcc ataggccata ggccaaggca
    13441 tctgcctttt ccaaacttct ctttttctcc aaccatttga agaaaacaag acgagccgtt
    13501 tcctcaaaaa atgctgccat ggctaggcca tagataatat agataagcgg atgatcttgc
    13561 aagagggcaa tactaccgtc tttttgaggg tgcaaaatca agatatgcac cagtttttcc
    13621 aaaatctgcg aagagacaaa gaaagcgact gcacccaagc ccaagacagc tagattaatc
    13681 tggtatttct ttttggcaaa ccaaatgctt cctatcagaa aagctagcaa cagcaacatg
    13741 gtaataatga tatgaatggt cattttcttc tcctattctg ccttttcaat atcttttttc
    13801 atctcgtcaa cattgaactt agcaaacaag tattgacggt cttggactgg gaaacgttgg
    13861 tccaactggt caactgctcc cacctcgata atgccttcct tgatgacaaa atccatgaca
    13921 tgtccaccga aggtcaaatc atctgatatg aagtgcagat ggtagcctgc cacactgacc
    13981 ccatggaaaa tctcaggcgt ccagaaacca acaatagtcc ccgcaacatt gtcacgacta
    14041 tattccggtt gatgggttgc gacatcagca aacttggtat cgggtgttga cttaggaatc
    14101 atacgcacat gcatatgtaa aaattccccc cgaatcttga tagagcggaa aagattttcc
    14161 ccatcataat aagactcaat tcgttctttc aattccttgt ctgtcatctc aaagcgctgg
    14221 cggaaaatga cctctgcctg atgcggtacc actgcagcgt aaggaataag ggcatctggt
    14281 gacacttcca caatttctgg ctggtctcct gaccctttgg cctgataagc cttgccatcc
    14341 aagacaatca attccccatc aatggaatcc aaggttccca aaccaaggtc accatgttct
    14401 agcaattctc ctactgtcat agtaccacca taaaggccag ccatcaaggc tccaagagta
    14461 ttgtattgaa ataatttcac tggttcctgc acgttcttat ccattcactt tcttgtctgt
    14521 tattctataa atcttactcc taagtatacc acatttgccc ctagatgtga acgagagaaa
    14581 cgctctagac attgccaaga aggaaaaaaa agggtacaat gtaacaaaat caagggaggt
    14641 ctggaatgaa gaaacaaagc aagtacaaag aggtcgtttc ctatctgaaa aatggtatcg
    14701 agtctgaacg atttccgacg ggtagtcgcc tgccttctat ccgtcaactg agccttgact
    14761 ttcactgcag caaggacacc attcaacgag ccctgctgga attacggcac gaacaatacc
    14821 tctatgccaa gcctcagagt ggctactatg tattagaaca agggcaacat caagacctag
    14881 aaatcgaggt taccgacgaa catgccagtg cctatgacga tttccgactc tgtgtcaatg
    14941 aaaccttgat tggacgagaa aactacctct tcaactacta tgacaatcaa gaaggattag
    15001 aagacctaag acagtccatt cacaaactcc tatttgagca agctctctac tgcaaggcta
    15061 accaactagt actgacttct ggaacccaac aagccttgtt tatcctctct caaatatcct
    15121 ttcctagaca agccaaggaa atcttggtgg aacagccaac ctaccatcgg atgaatcgcc
    15181 tcttgattgc acaggggctg gactatcaaa cgattgaacg aggcattgat gggattgact
    15241 tggaggagct ggaaggccac ttcaaaacag gaaaaattaa atttttctac accattccta
    15301 gatttcacta tcctctggga cattcctatt ctgagcaaga caaacgatct attcttaact
    15361 tagctgccaa gtatgatgtc tatatcgtag aggacgatta tctgggtgat ttggactcca
    15421 agaagggcca aaccttccac tatcttgata cagaggagca tgtcatttat atcaagtcct
    15481 tctcgaccag cctttttcct gcccttcgta ttacagcact cattcttcca aatgctatca
    15541 aagaagcatt tgtggcctac aaaaatatcc tagactacga cagcaacctc attatgcaaa
    15601 aggccctgtc actctatatt gacagtcaat tgtttgaaaa aaatcgtttg gctcgcttga
    15661 ccaatcatga atcttaccaa aagcaaatcg aggaaaggat aactaaaaca ccttgtcccc
    15721 ttcctcatta tcccctacac gatggtttat tgctagacct gagacagtat cctaaaatcg
    15781 ccagtctcaa acacagtcaa ctgggcttgg acttctttga agaggcctat ttaagcacct
    15841 gtccttatca atttgccaag gtgtccctag acaatctgga aaatgtttta aactatttaa
    15901 aagcagaatt ggaatgacat ccaactctgc tttttcttat tcttcaactt cagttagact
    15961 tgctgctttt tcaagatagc tttggtaagt tccttcatcc ttaagttttt ggataacctt
    16021 gtctaccact tctttcaaat catcactatt ttttctaaga gcaacggcat tggcgtcgcc
    16081 gtccttcatc ttcaagctga cagttgcgac agctaagcca gcgtttttag cagcataact
    16141 aagtgcaaca ggctcatcca tatgaacagc atctactttt ccaacctgca attcattgac
    16201 tgcttcaccc atattagtta gggaagttaa ttgagctttt ggcaattgtt ccttgaccat
    16261 tgattctgga acagtccctt tttgggctgc aatattagca ctttctaggc tagttaaatc
    16321 cttgtatttt tccacatcag ccttacgaac caagaaacta atcttgtttt catagtatgg
    16381 gattgaaaaa tcaaagactt cttttctctc gtcagtagca ctaattcctg caactgctag
    16441 gtcagcctta ccagtttgaa gactggtcaa aacattgtca aaactcatgc ttgagatttc
    16501 caacttaacc ccaagttcat cagcgatagc ctgagccatg tcgatgtctg caccgactac
    16561 ctggttcttt ccatcaacca atgattgaaa ttcaaagggt gcatagtcag gactggttgc
    16621 cacaactaat ttcccttttt gtttgatagc atccacagca gactgagaac cattagaacc
    16681 tgactgacaa gctactaaga agaaacttgc aagaaaatta cataaaacaa atatccattt
    16741 ttttgatttc ataaataaac aacctctctg ttaattttgt ataattataa cgatatccaa
    16801 gttacttgtc aagtgttttt taaattttta tctcaaaaat attttttcgt tcaaaaaaag
    16861 gagccatcag ttgatttcaa gctccttttt atacagaatt aaactatttt atagttcgac
    16921 aatcttacct gtttcaaagt agacaaccca ttcacagata tttttagcat agtcaccgat
    16981 acgctccaag taggaaataa cttggaaata atcacgaccc gtaacaatgg cttctggatt
    17041 tttcttaatc tcttcagtcg caaggtcacg gatagtttca aaatagtggt taatttgctc
    17101 atccatggag gccacccggt atgcgtcgtc aacagaacca ttaagataaa gatcaagtgc
    17161 tgcttcaaca acgcttttaa cttcacgtcc cattttttta atttcttcct ctacagctgg
    17221 aatgcgctct tcccccttca tacggatggt tgcctgggca atggctacag cgtgatcccc
    17281 catacgctcc acatctgata cagccttaag gacagtcaag actgtacgca aatcttgaga
    17341 gactggttgt tggagtgcga tcatttcaaa tgatttcttt tccagtttca cttcgtattc
    17401 atttacttct gcatcatctt cgatgacctc ttttgccagg tcacggtcat gcgtgacaaa
    17461 agcacgtacc gtacgattga tttgtgagag cacttcttgt cccatagcgt agaactggtt
    17521 atgtaatttc tctaaatctt cttcaaattg agatcgtaac atctttcatc tccttatcca
    17581 aattttcctg taatatagtc ttccgtttcc ttgtgttggg gatcaaggaa catctgcttg
    17641 gtatcattaa attcaatcaa atctccatct aggaaaaatc ctgtcttatc agagatacgt
    17701 gaagcttgct gcatggaacg ggttaccaga agcatggtgt acttgtcttt tagaccatac
    17761 aaggtttcct caattttacc agctgaaatc ggatccaaag ccgaagttgg ctcatccaag
    17821 aggatgattt taggactagt tgccaagaca cgggccacgc agacacgctg ctgttgacca
    17881 cctgacaatc caatagctga atcatataga cgatccttga cctcatccca gatagaggca
    17941 ccttgcaagg ctttttctac ggcttcatcc agaacctgct tatccttaat tccattgata
    18001 cgaagcccgt agacaacatt ctcatagata gtcataggga aaggattagg ttgttggaaa
    18061 accattccga tttccttacg taattcaacc gtatctgtac gcggactgta gatgttgtgg
    18121 ccattgtaca ccacggatcc agttgtggtc acctctggat tgagatctcc catgcggttg
    18181 agagacttga ggagggttga cttccctgat ccagatggac caatcaaggc tgtaatttcc
    18241 ttaggttgga aagataggga aacactattc aaagccttct ttttattata ataaacggac
    18301 aggtctgata cctgtaaaat cgcatctgtc atacggtttc ctttctaacc aaagtgacca
    18361 gatacatagt cattggtgga ctgtagcttg gcattttgga aaatagttgc agtcttgtca
    18421 tactcaatca aatcacccaa gtaaaagaag cctgtgtagt cacttgcacg agcagcctgc
    18481 tgcatattat gcgttacaat gatgatggta aagtttttct tgagctcaaa catggtctct
    18541 tctagttgca tggtcgcaat cggatccaag gctgaggctg gctcatccat taagaggata
    18601 tctggcttaa cagagatggc acgagcgata cagagacgtt gttgctgacc acctgataag
    18661 gtcaaggctg acttgtggag atcgtcttta acctgatccc agagggcagc ctgacgaagg
    18721 gaggtttcta cgatttcatc taggacttgc ttatccttaa ctccagcacg ttcatgcgca
    18781 aaggtaatat tacggtaaat tgacttagca aatggattgg ggcgttgaaa aaccattcca
    18841 atgtgtttac gcatttcata aacgttgatt tctggacggt tgacatcaat tccacgatag
    18901 agaatctgcc cagttacttt agcaatatca atagtatcat tcatgcgatt gagactgcgt
    18961 aagtaggtag atttccccga tcccgacgga ccaatcaaag ctgtaatttt atttctttca
    19021 aattgcatat caatcccctt aatggattca tttttaccat agtaaacatg gacatcctta
    19081 gtagaaaggg ctactttttc ttcaggaaag gtaaggatat gcttctcatc ccagttatat
    19141 gttgacatgg cttctccttt aggcagcggt taatttcttg tgtagatagc ttccgaactt
    19201 acgagctcca aagttaaaaa tcaggataaa gatcaggagc acagcggcag aacctgctga
    19261 tacaatggtt ccatctggaa tagtgccttc actattgact ttccagatat ggacagccaa
    19321 ggtttctgct tgacggaaga tagagatggg gctagtcaca ctgaggatat tccagttaga
    19381 ccagtcaaga gctggcgccg attgccctgc tgtatagatc agagctgcag cttcgccaaa
    19441 gatacgacca gatgccaaga cgacacccgt tacaatacct ggaagcgctt ctggaataac
    19501 aacatgaacc actgtctccc agcgagaaat cccaagagcc agaccagcct cacgttgggt
    19561 atggtgaacg tgtttcaaac tatcctctac attacgcgtc atctgaggca agttaaagac
    19621 tgtcaaggcc aaggcacctg aaatgattga aaatccatac tcaaactgga ctacaaagat
    19681 caagtaacca aagagaccca ccaccactga tggtaaagag gacaaaattt caatacaagt
    19741 ccgcacaaag ttggtaacag gacctttttt agcatattca gccaagtaaa tcccagctcc
    19801 catagaaaga ggtacagaaa taatcaaggt aatgaccaat aggaaaaagg aattgtaaag
    19861 ctgaatgcca atcccaccac ctgcttgaaa agcagaagac cttccagtca agaaagacca
    19921 agagatatgg ggcaagcccc gaaccaagat atagagaatc aaggaagcca agattgtcac
    19981 aatgatgcta gcaatcgtat agaggacagc tgttgcaagt ttatctaatt tcttagcgcg
    20041 cataattttt ctttcctctt tctttcgtaa tcaatttaat cacactgtta aaaactaaac
    20101 tcatcaagag cagtaccaag gccagtgacc agagaacatt attatttaca gttcccatga
    20161 cagtgttccc aattcccata gttaatatag aagttaaagt tgcagctggt gtggtcaagg
    20221 aagttgggat aacagctgag tttccgacaa ccatctggat agctagagcc tcaccaaagg
    20281 cacgcgccat cccaaagacc actgcagtga aaataccaga acgggccgcc ttcaagatca
    20341 cacgccagat agtctgccag cgagtggctc ccatagcgaa actggcttca cgataataac
    20401 gaggaaccgc acgcaagcta tccgttgtca taaaggttac ggtcggcaaa atcatgacaa
    20461 agaggacgga aatccctgac aaaatcccaa aaccagtccc accaaagaca ctgcgaacaa
    20521 agggaacgac gacttgcaag ccaataaatc cgtacactac tgagggaatc ccaaccagga
    20581 gttcaatagc tggttgcaaa atcttcgccc cttttggtga tacttcggtc ataaaaactg
    20641 ctgcaccaat agcaaagggt gttgcgataa gggctgagag aatggtaacg ataaaggaac
    20701 ccaaaatcat aggaagggca ccaaattctt tactagaagg attccaagtt cctcccaaaa
    20761 gaaagtcaaa gatattcaca ccattgacaa agaaggtcga caagcctttt tgcgctacga
    20821 aaaccaaaat catggccaca aggatgacta tcaaagaaag acaggcaaag gtcaaacctt
    20881 ttcctaattt ctccagacga gaattctttg atggaagcaa cattttctta gctaattctt
    20941 cttgattcat tattgtctcc cttccaacac tgtcacagtt ccggcagcat ctttttcaac
    21001 cttcatttcc ttaatcggaa tatacttcaa tcctttgaca atcccttctt gggtctcatc
    21061 cgagagaaca aaattgagaa attctgcagc caactcattg ggctgcccca atgtatacat
    21121 atgctcataa gaccacaagg gccaattatt gctacttata ttttctggac ttaagtcata
    21181 gccattcaac ttcatgcttt tgaccgaatc atctatatag gtaagagata aataagagat
    21241 agctcctgga ctttttgata cgattgattt taccgctcca tttgaatcct gctcctgact
    21301 ttgcatggca gactgacctt ccataatgac agtatcaaag gtagcacgag aaccagagcc
    21361 ggctgcccga ttgataacag agatgggtaa gtccttacca ccaacctctt tccaattggt
    21421 tacctcacct atgaagattt gacgaagttg ctctgtcgtt aggttatcaa catcaacctc
    21481 cttattgaca atcagagcca agccagctac cgcgaccttg tggtcaacaa gagcagaagc
    21541 atcaattccg tctttttcct cagcaaatac atctgagttt cctatatcaa ctgccccaga
    21601 ctgaacctgg gacaagcctg taccagaacc tcccccttgg acattgaccg tttttccaac
    21661 atggatcgtg ccaaattcat ctgccgctac ttcaaccaag ggttgcaagg cagttgagcc
    21721 aacagccgtt atggattctc cacgatcaat ccagctagca cagcctacta aacaagccgt
    21781 cagccaaaaa gcgataagag acagagcaag cttttttctt tttttcactg tttttctcct
    21841 cgaaaataat tatgaatact gtgaattttt taagtagttc tttatgagtt gacgcatgaa
    21901 ttcttaccaa atttctgcgc aattgattat ttatataata taggctatat tactctctcc
    21961 taacctcctt ttttcatatg tggataaaat ctcttgtcta tcccttcccc cattgtcacc
    22021 cattatagtc atttcgtgtc tctttttccc ctttttaatg caagggaaat tactctcctt
    22081 agatgataat ccaaaagcta gaaaggtatc tcaaacctct ctactctccc agactagttt
    22141 acaactaaaa ggaaaagatt ctattttatg agaaatctag tttacaagcg gtaagaacgc
    22201 taataactaa acttcttgta ctctttgaaa atctcttcaa accagtgttt tgagctatct
    22261 atggctagct tcctagtttg ctctttgatt ttcattgagt agtaaaacta catgtaatgg
    22321 caatcaagat atcaagaatc atcctactaa aaaaatccat actttcacta taacatagaa
    22381 taagatattt gactagcatt ttcatttgaa tctgaggcct tttggaaaat aatttttcaa
    22441 aacatttcca gtaacctttg caaagcccaa gccattgcct ttaaccaaaa cttggtacca
    22501 accatttggc agactttctg ccagctgaac gatttctcca gccgcatact tgacaaacgc
    22561 ttcttggcca atttcaaccg actgttcgac ctgactcgat ttcaaggcta aaccaagagc
    22621 gaaactgggc tcaaagcgtt tcttcttaaa agtacccaga tgcagtccat tgcgagcaat
    22681 cttgagcttc cctaaatctg gcaaaagttc tggcaagaga taaagctggt ctccaaaagt
    22741 ctgcaagata cccggtagat tgaccttcaa atggttttgg gcaaattcct gccacaaggc
    22801 aacttgttca cggctgaggt tactcttact tgccttaaat ttaggagctg gattgttacc
    22861 cttaaactgt agatgggcaa caaactgacc ctctccctta aactgatgag ggtacatccg
    22921 agccgtttct ggcaggtcaa taccagctac cattccattg atatgctcta ctggcaacaa
    22981 gtcaaaatca tactcttcca gtaaccaatt gacaatctct tcgttttcct cgggtgccca
    23041 ggtacaggtc gaataaacca gacgaccacc ttcagctaac atggtcactg catcctccag
    23101 aatttctctt tgcaagctag cacattgact cggataatct aagctccaat agtccatagc
    23161 atcaggttgc ttacgaaaca ttccttcacc agagcaaggg gcatcaagaa cgattaagtc
    23221 aaaatagcct ttaaagacct tgaccaagcg gtcggcagat tcattggtca ccacgacatt
    23281 tgtcgctcca aaacgctcca tgttttcaac caaaatctta gcccgtttgc ttgaaatttc
    23341 attggaaaca agtagcccct cccctgctag ataggctgcc agttgagttg atttgccccc
    23401 cggtgcagca gccaagtcca agaccttcat accaggactg ggttgagcta cttgagccac
    23461 catttgagca gcaggttctt gcgaataaac taaacctgta gcatgctcag gcgatttccc
    23521 tgaaaccttc ccatagtggc cccaaggggt ttgagtaatg gcatcagaaa aggaaagttg
    23581 ctcttctttt aagggattga cccgaaaggc cgaaaccgct tcctcctcaa aagaggcaag
    23641 aaaatctctt gcctcatctc ctagtatctc tttatatttt tcaacaaatc cttctggaaa
    23701 ttgcatttaa gttcttttcc tttcgtaaat ataggactga atttcctcct gcatctcaag
    23761 aggcaccatc atgaccggct gtctggtttg aaaatcagga gcttcaccaa aaagggtcac
    23821 aacccgatag cccagacttt cccctaaaat actagctgcg gcataatccc atggttgcag
    23881 ataagtgaga taggtcaaca aacgccctga caaaatcttg gcaaaactaa tggccgcact
    23941 tccatagaca cgaacaccaa gaaccgctcg gctcaaatca gccagccccc attcattggt
    24001 ttccagcata ccactattcc ctgcaatgag aaaatctcca agtggtttag ttttaaaagg
    24061 agctaggggc ctatcattta gacaaactgg aaattcccca ccaccgtggt aacaatcccc
    24121 tttgaccaca tcataaatca gaccaaactg tccctgacca ttttcaaaat aagccatcat
    24181 aacagcaaaa tcttcctgct gggctacaaa attattggta ccatcaatgg gatcaatgac
    24241 ccaaaccttg ccctcttgaa ccgaggctcg cagacaacct tcttcagcac aaatcttatc
    24301 ctcaggataa cgggacaaaa tctcaccaac caagagttcc tgaacttctt tgtccagtct
    24361 ggtcaccaaa tctgttggag aggacttggt ttcaacacgc aagtcttcct gcatatggtc
    24421 aagaatgtac tgacctgctt tcttaacaag ctctttagca aattcaaatt tactttccaa
    24481 gagaaatctt tccttcccct ttttctttgg cagcctgaac tgctcggtaa agtgaatagc
    24541 cactaaccgt ttcaaattct cgtcccaaac gtttctcttc gctcttgctt ggcacgaccg
    24601 ctttgaatcc cttataggag tccagtaact ttttagcctc tactttgcct tcataggcag
    24661 cttcaacatc attaaaaaaa gaaagcactg aagcaagttc ttcagtgctc cacgacaaat
    24721 ctagtgggta actatactgt ttgttcatta actaatacca gctctcattc ttgcttcttt
    24781 tagttcttgc ttacgataac tacgagggag aaaagcacga atctcatctt cattaaaacc
    24841 gatttgcata cgcttggcat caataataat tggacgacgc aaaagactag gatactgctc
    24901 aatcaaatga agcaattccg ataccgaaat actctctaca tcaatattca atttttgaaa
    24961 aatttttgaa cgagttgaaa tgatgtcatc agtaccattt tcggtcaagg aaaggatgtg
    25021 ttgcaattct tttcttgtta aaggactggt cataatattg tgttccacaa agggaacctt
    25081 atgtttttct aaccaggcct tagccttacg acatgatgta cagctcggtg atagaaatag
    25141 tgtaatcatg cttttctctt cttatctata ctttgctact tctattatac aaaaaaataa
    25201 ggcgcttgac tagggatttt tagaaaaaaa gcctattttt tcaagaaaaa taggctgttt
    25261 gcgaacgatt gacacaattg gatttggtta attcactctt aacgatggtt ttaaacgata
    25321 tatattttta tatatgcaaa ttaaaaacat ctttcatttc acttcctacg acttttcaga
    25381 cacagatagc caaagaagct ttcatagagg gcaaaaaaga ggaggaaggc atgaagaaag
    25441 aaggtctctg gcaaaatcat aataacagga tccttggctg gatcaaaaag ccaggtatca
    25501 tctcccacaa agagaatttg atggaaaaga gtaaagaatt ggtcaaaacc aatcaaaact
    25561 cccccaagtc caatcatcac aggtaagact actagagcca ggagactttt tcgataaaga
    25621 gacaaaaagt cctttttcac aatcctattg acaaagacat agaaacttgg cagtgtcact
    25681 agagctacta gctgaaccaa atgaaagaga ttcttgacca ctgcgaaatg gtgcagacca
    25741 gctgctgacg aacgaaaatc aggcatctgt aagacctgac taaaaggatt ggtcagataa
    25801 ttcatcaaga tatgaaaatt gtattgaatg gtttctggtt ttagatagac tcgattcgtt
    25861 aagtttagcc actgaatctc cataggatag aaaatccaag ccagataaat ggtcagaagg
    25921 attgagaggg agaggagaaa gagcatagag ccccaaaagg tcaatttagt tttcatcaaa
    25981 atcccactcc gcaaggctag aaaccacatg tgtcggtgcg attggcaggc cagctacttc
    26041 ttctgccttg gtaaaacctg tcgtcaccaa gagcgttgga atgccattgt caatcccagc
    26101 ccggatatca gtcaaataat tgtccccaac catgattaac tcttcacgtt ccaaacctaa
    26161 gtgctcaacc gccttgtcca taatgatggc atttggtttt ccgatataaa ccggcttcac
    26221 tcgtgtcgct acttcaagca gcgtaatcag tgagccagca cctggcaaaa gaccgcgttc
    26281 cgtcgggatg ttgaggtcag gattggttcc gataaaatgg gcaccctttt gaatagcaag
    26341 agttgctgtg gcaaattttt catagtcgac ttgccaatcc agaccaacta ccacgtaggc
    26401 aggtttttcc ttgtcttcca cataaccagc cgccttgatg gcttccttga gtcctgcttc
    26461 tccgacgaca tagacggtct tttcaagccc caaatcattc atatagtcga tggttgccaa
    26521 agtcgctgtg tagacagtcg ataggggcgt atcgatatta aaattctgag ccaacatctc
    26581 cttaacactc tctggagtgc gggttgtatt gttggttaca aagagatagg gaatgtcccg
    26641 cttttgcaat tcatgaacaa aagtctctcc agcagggatt cggtctttcc ccttataaat
    26701 ggttccgtct aaatcaatta aatagccttt atatttcatc tatttctccc taagcctttt
    26761 ttatttcttg ccaagtaatg attgcttggg cattgataac cccatcactt gtaatttcat
    26821 gcttgctttc cagtccagtc cgttcaacag ccgatgtaat caccccacct ggtcgaactt
    26881 ccttgacata cttgaggttg attttcttgg gaatatagtg ggtcaaaaaa tccgctccca
    26941 tgacctcaaa aatccagtcc aagtatttac tgttattgac atgaccattc atatccaagt
    27001 cgtaaaaacg aacatggtaa tccttgctga tcggttcttc caaggactca tacttcggtc
    27061 cacggataag ttttttatca aaatcagact ggtaaggagc cacaatctca ggttcaacaa
    27121 catggacttt tcgactgtcg cggtccatga gaacaaaggt cgccatcatg tggatgagct
    27181 cctgctccgc ttcattataa atagtaaagc gacggtagca aaaaagtcga ttgtagctca
    27241 aggcttccgt ttcgatggta atttcttccg caaaacgagg caaacgaacc acctcaatat
    27301 catattctac gataatccag accagattat attcttccaa aatggcctta tcactaactc
    27361 ccagttcaat cgactgcatc cctgaaactt gcagtgacag caaaatcaca tctggaagtt
    27421 tgatatgacc gttcatatca gccatatcaa aaggaatttt cattttcatt tgataagtta
    27481 agcccatgat cctactccaa aataaatcgt tctgctacag tatctcccaa aaagagacct
    27541 ctctttgtca tgcgaacgtg gtcaccctca atctgcatga ggccttgttg aaccaaatct
    27601 ctgacaattt ctccataaag tccagcaaaa gactgtccaa atttttcctc aaatcgcgcc
    27661 atggaaaccc cggatttctt gcggagtccc aagaacattt cttcttccat ttgctccttt
    27721 tgactcaggt gatcttctgt aatacaagca ttgccttcct caaccgcact gagataatga
    27781 cgaatgggac catgattttt atagcgtact ccattgacat aaccagatgc ccctgcacca
    27841 ataccatagt attcagcatt gtcccagtac atgagattgt gacgactttc aaaaccgggt
    27901 ttggagaaat tagaaatctc ataatgctca aaacccgctc gctccagctc tgcaatgatg
    27961 tactcaaaca tctccgcttc tagttcctcc ttaggcagag gcaatttccc acgtcgcatc
    28021 cggttcataa agaccgtatg gttttctaaa atcaaactat acaaactcat gtggggaata
    28081 tccagtccaa tggctttagc cacattttcc tttacttgct ccatggtctg accaggcaga
    28141 gcataaatca aatcaatgga gatgttgtca aaaccagcca gtttcagacg atcgatattt
    28201 tcataaatat ccttctccaa atgactgcgc ccaatctttt tcaacatctt atcatcaaag
    28261 gtctggacac ctagcgaaac acgattgaca gccgaatttt tcaaaacagc tatcttatcc
    28321 gcatccaaat cgcctggatt ggcttcaatg gtcaactctt ccaagacaga caagtccaag
    28381 tttttagtca agccattcag taacacctcc agttgcggag ccgacagggc tgtcggtgtt
    28441 ccaccaccga tataaagggt tgacaacttt tcaatatcat aagaacgaaa ctcttccagc
    28501 agatgctcta aatagctgtc gactggctga tttttgatga agacctttga aaaatcacaa
    28561 taataacaaa tctgggtaca aaatgggatg tgcacatagg ctgacgttgg ttttttctgc
    28621 atagtaatta ttataccaca aagactagat tccagataaa aatcaccatc cccagataca
    28681 tagtccgtcc ggagatggtg atggtttatt cttctgttat atcaatcaca atctcttctg
    28741 agtcatcaag agcttcggct ttttcttgcc attgttcctt gagattattt aattgatttt
    28801 ttgatgcttc tgtcgcttga aaagcatagg atttagcttg agcaagtata ctatccacag
    28861 tgatttcacc tgactcaacc tgttcttttg ttttcagaac aaaatctgta gcctgctcct
    28921 taacttctgt cagtttttca cagacttgct ccttggcata ctccggatct tctctcaaat
    28981 catctagaaa atcttgagcc tgactgcaaa cttgtttgcc cttatcactt gttaaaaaca
    29041 aggcaagagc tgcacctgaa accgttccta aaaggattga ggataattta cccataagga
    29101 ttctcctttt ttattttttg aaaaatttac ttgcaagacg aagagctgac agacttgcac
    29161 cagtcttgag tgtttttgaa ccagctgatg aagctttctt gctcaagaca cgcgcatggt
    29221 cattgaggtc tgaaacagat agagataaat ctgcaacagc gctgaagagt ggatcaatcg
    29281 tagccacctt gacattgata tcatctgcca agacattgac cttagccaac aactcattgg
    29341 tgtgatgcaa ggtcacatcc acatctgaag tcaaggtttt aatcgtcttt tctgtttcat
    29401 cgatgacacg accaagcttt tgtacagtaa tgatcagata gaccaaaaag acaatcaaag
    29461 ctagggcaac aagaatatat gcaacttcta acatttattt ttcctcctct gtaatatagt
    29521 aaggggcctt ctttcgattt tgataaataa cgatcattat accgagaccg ataaggacaa
    29581 ctgacagcca ttgggacact cgaaggctga agaacatgag actatctgtt cgcatacctt
    29641 cgatgaccat acgaccgaaa ccataccaaa tcaagtaaaa ggccgtgata tgacctcgtc
    29701 tgagactctt ccatttccgt ctaaaaatca gaatcaaagc aaagccaagc agattccata
    29761 gagactcata aaggaaagtc ggttgacggt agctcccctc aatatacatc tggtcacgga
    29821 taaagccagg tagataatcc agattatcca ctgttgcacc ataagcttct tggttaaaga
    29881 aattacccca acgccccaaa ctttgagcaa tcataacgct aggcgccgca atatctagaa
    29941 aatcccaagt attgatgagt ttacggtcag caaagatata gagcacaaga gccccagtta
    30001 tcaaaccacc gtaaatggcc aaaccaccat tccaaatggc aaaaatctct cctaaattct
    30061 gactatagta atcaaatcgg aaaataacat agtagagacg agctcctaaa atagccaagg
    30121 gaaaggctac taagataaaa tctaaaatat cgtctggtat gatcttcttt ctaggtgctt
    30181 ctttcatggt caaataaacc gcaagaatca agcctgtcac aatacataag gcataccaac
    30241 gaatggctag gggtcctagt tgaatagcaa ttggatcaag cattttgcac ctcatttcga
    30301 gcaatgagac ttgtcagtcg ttcgtcgaac aaacgggtcg catcaaagcc catttccttg
    30361 gcacgataat tcatggcagc tgcctcaatc acaacagaga tattacgacc tgttttaact
    30421 ggaatacgaa tacgaggaat ggctacgcca gaaacttcaa gttcctctgc attgtttcca
    30481 agacgatcaa aggtcttatg tgtatcgtaa ttttccaaat agacagcaag ctgaacctgt
    30541 gaagaatcct tgacagcact cgcaccgtag agactcataa catcgataat accaacccca
    30601 cgaatttcaa tcaagtgttt caaaatttca gctggttcac cccagagagt aatctcatcc
    30661 ttggcaaaga tatcgacacg gtcatcggct accaaacggt gaccacgttt gacaagctca
    30721 agacctgtct cgctcttacc aattccacta tctccctgaa tcaagacgcc catcccataa
    30781 atatccatca agacaccgtg cacactggta cgttctgcca aacgagaatc cagatagcta
    30841 gataactctc cagataaacg actggtagct gcacggctgg ttaaaatagc aatcttacat
    30901 tctctagcag cctttaacat ctcctctgga accaccaaac cacgagcaac aatgactgct
    30961 ggtgtctcag gtagaaacat ttttttcaaa acttcataac ggctgttgga aggcatgctg
    31021 atcagataag accactcctt catccccaaa agttgaatcc gctctggtgt atagtagtca
    31081 aaatagcctg tcatttcaag accaggtcgc gtaatatccg ctgtattgat ttccttttca
    31141 agcaactctg gttcaccata gacaatgtct agtctaagct tttcaatcac ttcttttact
    31201 aaaaccgaca tcatttcctc cttcaaatga gttcttctct ctattatatc aaattgtagg
    31261 aggaagtttc agatttttgc aggctttgga gtatttattt aaaagaaaaa ggactagatt
    31321 tctctagcct ttcatttcaa ttagaatttt ttacgtttac gagctacttc tgatttacgt
    31381 ttacgtttta cagaaggttt ttcatagaat tcacgtttgc gtgtttcttg aagagtacca
    31441 gctttagtaa ccgcacgttt gaaacggcga agtgcgtcgt caagagattc attcttacgt
    31501 actactgttt tagacatttt tttcactccc ttcaagttca atatctattt cattctacac
    31561 gtaaaaggaa taattgtcaa gcaaaaatgc ggtttaattt tgattttttc ttccatttat
    31621 acaagagttg aaagcgttca atagaacatt gcttttttat ttttcgagta agctaagcgc
    31681 ttcagcatct gcgatgatgg ttacatcagg gtgattttga aggctacttg caggtaggtt
    31741 ctcagtcact gggccagata ctgttccggc aatggcttct gctttcgact caccgtaagc
    31801 aaaaagaata atagacttgg catccaaaat gtttttaatc cccattgaaa tagcttgggt
    31861 tgggacgtct tcaatcttgg caaagaagcg tgcattggct tcgatagtag actggtcaag
    31921 ttctactaga tgcgtttgac tgtcaaatgg agtaccaggc tcattaaagc cgatatgccc
    31981 attgcgaccg attcccaaga tttggaggtc aactggatgg tcagccaaaa tttggttgta
    32041 gcgttcaact tcagcttcag cattgtcctt aacaccacga ggcaagaaac tttctttaaa
    32101 tggtttttgg ttgaacaagt tttcttgcat gaagtaacga taagactgtg gattgtcccc
    32161 atcaaggccc acatactcat caaggttgac actggttaga tttgaaaaat caaggtcact
    32221 ctcaacaatt tccttgtaaa attcaagtgg gctacttcct gtcgcaagtc ctagagtttg
    32281 agctccatta gccaattttt ccttcaaaat ctcaaaagca acttttccac cttgaacttg
    32341 gttttcaact ttaataactt tcatcttttc atcctccatt tctgtctata tttattatat
    32401 ggtatagacc aatttttgtc aagtgaaaac gatttaattt ctaaaaaaag agcgtccaaa
    32461 cttgaaacgt gttataatgg ataggattat aacttagaaa gaatgaacaa atatatgaat
    32521 acagctgatt ttgatttcca cttgccagag gaattgattg cccaaacgcc ccttgaaaaa
    32581 cgtgatgcct ccaaactcct catcgtcaac cgtgagacag gagaaatgca agataaacat
    32641 ttccactcta ttattgatat gctggaacct ggtgatgccc ttgtcatgaa cgacacccga
    32701 gttctccctg cccgcctcta tggtcaaaaa gtggagacag gaggtcatgt ggaacttctc
    32761 ctccttaaga acactagtgg agacgagtgg gaagttctgg ctaaacctgc caaacgcctc
    32821 aaggtcggta ctcgtatcag ctttggtgat ggccgcctca gcgctgtcgt tacagaagaa
    32881 ttgacccacg ggggacgcat tgtccgcttt gaataccaag gaattttcct agaagtcttg
    32941 gaaagtctgg gagaaatgcc tctgccacct tatatccacg aaaaattaga tgaccgtgaa
    33001 cgttatcaaa ccgtctacgc caaggaaagc ggctctgctg cagcaccgac tgctggtctt
    33061 cacttcacca aagaactgct ggcagaaatc caagctaagg gtgttcatct agtctatctg
    33121 actctccatg tcggactcgg aacctttaga cctgtttctg tggataatct ggacgaacac
    33181 gaaatgcact cagagttcta tcaactttct gaggaagctg ctgccaccct tcgctctgtc
    33241 aaagaaaatg gtggtcgcgt catcgctgtc ggaaccactt ctatccgcac cttggaaact
    33301 attggttcca agtttgatgg gcaaatccaa gcagattctg gttggaccaa tatctttatc
    33361 aaacctgggt atgagtggaa ggtcgtggat gccttctcaa ccaacttcca tctgccaaaa
    33421 tcaactctgg tcatgttggt ttctgccttt gcaggccgtg aattagtctt agatgcctac
    33481 caccatgcca tccaagaaca ctaccgcttc ttcagttttg gtgacgccat gtttatttat
    33541 tgagaaagaa tttctctaat tcttctaata ccaataaatc gctaagatat tatttcaaag
    33601 aacatctaca attgaaactc tagctagctg tagaagacgc ctagtacatt gaaattaaaa
    33661 tgcttccccc tagcttcgaa aatattgcca tagattgcgt tgactctcca aattgattca
    33721 tctatatttt atttcagctt cctatatttt cttcgctgtt tgtaaatcaa aatgcaagac
    33781 acatgagtag caccatattt gttactctta tctgtcctct caagagacta ttatgagtta
    33841 tttcagaatc attcactact ttgaccctga ctctccttag tctcaaaatc aaagacttat
    33901 actcttcaaa aatctcttca aaccgcgtca acgtcacctt ggattatata tgtgactgac
    33961 ttcgtcagtt ctatctacaa cctcaaagca gtactttgag caacctgcga ctagttttct
    34021 agtttgctct ttgattttca ttgagtatta aacaaaaagt gaacaaatct gaattctaat
    34081 gtacagaaga ctaggcttgt tcactttttt atagtcgcta taagatgacc ttatctatag
    34141 ctttttatat ataattatat attcagacat actattatca attttgtcgc agggaggaat
    34201 ctgttaacgc acccattcac cattatcatt gactctatag ccatctatac ttgtattgac
    34261 cgctaactca cccgatgcat ttacataata ccatttacca ccaacttgga accattgatt
    34321 gactttcata gaaccgttgc tgttgaggta gtaccatgaa ccattaactt gtacccaacc
    34381 tgttgccatg gaaccatcag tattataaaa ataccacata ccattttctt gtttccagtc
    34441 tgttgttgga gcaactgctt tagctggttc tactgctaca tctgttcctt ggttagatgt
    34501 aacagataca ggatacgaag gaatagatga ttgctcagga acaacaactt tttcaggttc
    34561 tctcgtccct ctccttatac gtctttttac catctcttta gtaatttgac gagaagtagt
    34621 ttcttcaatt gttccatcac gttcatctac agtatagatt gtagtaagag taatttacca
    34681 atttctccta cttcttctac ttcttgactt ttatcaagag ttgggccatc gagatattct
    34741 gtttcgattg gaatttcttg gacaagaact tggggcttgg ttcttttttt aacaactctt
    34801 gtttgagagt ctttttttga cttaaagtac tctcagttac ttgtccactc tttccatcta
    34861 cattataagt tatcgttgta actgttttcc cattctttcc tagagtaatc tcttgctcct
    34921 gtcctgcaga aaggtcattg tctgcttcat atttagtagc aaatggaaca agaacttctt
    34981 caaccttgct tttagctgga actttgataa ctgtatccgt ggcttctttt ctatcaacag
    35041 taacctgttc ggtaacataa ccagtctctg gattaacatc gtaggtcctt gtcgtagtta
    35101 catagccatc ctctccatca attgtaacag gattttcact acggtctttt gtttcatctt
    35161 tttcataacg aattcgcgta cttgaaattt tcttggttac taccttaggt ttagtcgcta
    35221 cttttacaat aatatcccca ttgtcagcgt catcatactc tattccctct tctttatctc
    35281 tagtatcatc tctgacatat tgaatcccat cagcagcatg aacaaaactt gtattcagat
    35341 tcctcctaaa aataaagtta gcccgattac cgcagaacca aaaatctttc cgagtttacg
    35401 tattgcatag cgcttattag tattagattt tgccattaca tcctacttct agtatagcat
    35461 cttttctatc aaacgttaaa caatatactt tatatataaa atagacttag aatgatatat
    35521 tgattattga actaacactt taactatatc gtaatcaatc tcatatataa aggattgcag
    35581 acatcttatc taaatacatg ctaatatatt tagatacaaa cattccaact tgataatttt
    35641 cactcatctt tcatcattcc ttatacaact atgcagtata aatagaatag ttttctca
//
LOCUS       DAVMGZ010000007        34555 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000007 DAVMGZ010000000
VERSION     DAVMGZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.007"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            57..1511
                     /locus_tag="VYQ93_000271"
     CDS             57..1511
                     /locus_tag="VYQ93_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV3335025.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGIYYELDYGHKTN"
     gene            1492..2457
                     /locus_tag="VYQ93_000272"
     CDS             1492..2457
                     /locus_tag="VYQ93_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3335026.1"
                     /translation="MVTKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(2492..3766)
                     /locus_tag="VYQ93_000273"
     CDS             complement(2492..3766)
                     /locus_tag="VYQ93_000273"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV3335027.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQIANPKLAQEKIASKKAELLELANQAYPSIVKRGGGASDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(3766..4938)
                     /locus_tag="VYQ93_000274"
     CDS             complement(3766..4938)
                     /locus_tag="VYQ93_000274"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV3335028.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(5069..5317)
                     /locus_tag="VYQ93_000275"
     CDS             complement(5069..5317)
                     /locus_tag="VYQ93_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335029.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(5385..6008)
                     /locus_tag="VYQ93_000276"
                     /pseudo
     CDS             complement(5385..6008)
                     /locus_tag="VYQ93_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6070..6519)
                     /locus_tag="VYQ93_000277"
     CDS             complement(6070..6519)
                     /locus_tag="VYQ93_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV3335030.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(6521..7279)
                     /locus_tag="VYQ93_000278"
     CDS             complement(6521..7279)
                     /locus_tag="VYQ93_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV3335031.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(7394..9373)
                     /gene="pknB"
                     /locus_tag="VYQ93_000279"
     CDS             complement(7394..9373)
                     /gene="pknB"
                     /locus_tag="VYQ93_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV3335032.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(9370..10110)
                     /locus_tag="VYQ93_000280"
     CDS             complement(9370..10110)
                     /locus_tag="VYQ93_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV3335033.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAYPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(10125..11438)
                     /gene="rsmB"
                     /locus_tag="VYQ93_000281"
     CDS             complement(10125..11438)
                     /gene="rsmB"
                     /locus_tag="VYQ93_000281"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV3335034.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNALLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(11431..12366)
                     /locus_tag="VYQ93_000282"
     CDS             complement(11431..12366)
                     /locus_tag="VYQ93_000282"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV3335035.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHAFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(12379..14775)
                     /locus_tag="VYQ93_000283"
     CDS             complement(12379..14775)
                     /locus_tag="VYQ93_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV3335036.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFDSEDSLAFSSLDLGKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVRRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(14841..15155)
                     /locus_tag="VYQ93_000284"
     CDS             complement(14841..15155)
                     /locus_tag="VYQ93_000284"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV3335037.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(15180..15806)
                     /gene="gmk"
                     /locus_tag="VYQ93_000285"
     CDS             complement(15180..15806)
                     /gene="gmk"
                     /locus_tag="VYQ93_000285"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV3335038.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(15936..17549)
                     /locus_tag="VYQ93_000286"
     CDS             complement(15936..17549)
                     /locus_tag="VYQ93_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV3335039.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(17740..17994)
                     /locus_tag="VYQ93_000287"
     CDS             complement(17740..17994)
                     /locus_tag="VYQ93_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEV3335040.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(17998..18252)
                     /locus_tag="VYQ93_000288"
     CDS             complement(17998..18252)
                     /locus_tag="VYQ93_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV3335041.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(18490..19587)
                     /locus_tag="VYQ93_000289"
     CDS             complement(18490..19587)
                     /locus_tag="VYQ93_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV3335042.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILARLGIDSLAFGTEEVLD
                     YQKIVDLYTERGAEMESFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVDKDVDFASATALRQHQSDQDFLERFMPSVALFEQA
                     SKVSWDDYFPLLRYQILLNPDLTTVYQVNQEMVVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARENVLPEVIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGNPSIADQNFGRVPIRIETN"
     gene            complement(19597..20337)
                     /locus_tag="VYQ93_000290"
     CDS             complement(19597..20337)
                     /locus_tag="VYQ93_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV3335043.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTDFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSGDMLKIAEKRASSAKQKIDFIEGNMLDLSKSGQYD
                     FVTCYSDSICYMQDEVEVGDVFKEVYNVLNEDGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEVLTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(20446..20799)
                     /gene="rsfS"
                     /locus_tag="VYQ93_000291"
     CDS             complement(20446..20799)
                     /gene="rsfS"
                     /locus_tag="VYQ93_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV3335044.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEADSVDISEALA"
     gene            complement(20812..21312)
                     /locus_tag="VYQ93_000292"
     CDS             complement(20812..21312)
                     /locus_tag="VYQ93_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV3335045.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(21313..21906)
                     /gene="yqeK"
                     /locus_tag="VYQ93_000293"
     CDS             complement(21313..21906)
                     /gene="yqeK"
                     /locus_tag="VYQ93_000293"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV3335046.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(21906..22535)
                     /locus_tag="VYQ93_000294"
     CDS             complement(21906..22535)
                     /locus_tag="VYQ93_000294"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV3335047.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(22587..22898)
                     /gene="yhbY"
                     /locus_tag="VYQ93_000295"
     CDS             complement(22587..22898)
                     /gene="yhbY"
                     /locus_tag="VYQ93_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV3335048.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(23113..24219)
                     /gene="yqeH"
                     /locus_tag="VYQ93_000296"
     CDS             complement(23113..24219)
                     /gene="yqeH"
                     /locus_tag="VYQ93_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV3335049.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(24222..24812)
                     /locus_tag="VYQ93_000297"
     CDS             complement(24222..24812)
                     /locus_tag="VYQ93_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV3335050.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(24764..25672)
                     /locus_tag="VYQ93_000298"
     CDS             complement(24764..25672)
                     /locus_tag="VYQ93_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV3335051.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(25729..26289)
                     /locus_tag="VYQ93_000299"
     CDS             complement(25729..26289)
                     /locus_tag="VYQ93_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV3335052.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            26428..27636
                     /gene="sstT"
                     /locus_tag="VYQ93_000300"
                     /pseudo
     CDS             26428..27636
                     /gene="sstT"
                     /locus_tag="VYQ93_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            27704..28657
                     /locus_tag="VYQ93_000301"
     CDS             27704..28657
                     /locus_tag="VYQ93_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV3335053.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYGQ"
     gene            28801..29181
                     /locus_tag="VYQ93_000302"
     CDS             28801..29181
                     /locus_tag="VYQ93_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV3335054.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRICCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            29280..29501
                     /locus_tag="VYQ93_000303"
     CDS             29280..29501
                     /locus_tag="VYQ93_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV3335055.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            29518..29832
                     /gene="trxA"
                     /locus_tag="VYQ93_000304"
     CDS             29518..29832
                     /gene="trxA"
                     /locus_tag="VYQ93_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV3335056.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            30137..30670
                     /gene="queF"
                     /locus_tag="VYQ93_000305"
     CDS             30137..30670
                     /gene="queF"
                     /locus_tag="VYQ93_000305"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV3335057.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQINREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            30732..30899
                     /locus_tag="VYQ93_000306"
     CDS             30732..30899
                     /locus_tag="VYQ93_000306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335058.1"
                     /translation="MFEVVDRTDEVSLKHCFEVADRTDEVSNHTYGKVKLTWFEEIFE
                     EYHTKKPCSSR"
     gene            complement(30924..31592)
                     /locus_tag="VYQ93_000307"
     CDS             complement(30924..31592)
                     /locus_tag="VYQ93_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV3335059.1"
                     /translation="MKKFVAELIGTFMLVFIGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAFSIGTVSGAHLNPAVSIAMFVNKRLSSKDLVNYILGQVVGAFIASGAVFLLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLIIGLS
                     LMAMILVGLNITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(31698..32488)
                     /locus_tag="VYQ93_000308"
                     /pseudo
     CDS             complement(31698..32488)
                     /locus_tag="VYQ93_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
     gene            complement(32913..33677)
                     /locus_tag="VYQ93_000309"
     CDS             complement(32913..33677)
                     /locus_tag="VYQ93_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3335060.1"
                     /translation="MFKLSFQSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TSDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            complement(33670..34440)
                     /locus_tag="VYQ93_000310"
     CDS             complement(33670..34440)
                     /locus_tag="VYQ93_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3335061.1"
                     /translation="MLYLWGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDSLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFLGLTGTEKEDLHV"
BASE COUNT         9767 a         7978 c         6728 g        10082 t
ORIGIN      
        1 gcatttttta tcgaaaacag ttactattaa aagtgataag atttttggag gaatgaatgg
       61 aatggacaac tgagcgtcgt tacagacttt atcaagattg gacgcaagaa gaaattcaac
      121 atataaagga aaatatggca caatctccat ggcatactca ttaccatgtt gagccaaaaa
      181 caggacttct caacgaccca aatggctttt cttactttga tggcaagtgg atcctctttt
      241 accagaattt tccttttggt gcagcccacg gtttgaaatc ttgggcacag ctagaaagtg
      301 atgatttggt tcactttaaa gaaactggaa tcaaagtttt accagatact ccattagata
      361 gccacggtgc ctactctggt tctgccatgc aatttggcga taacttattc ctattttata
      421 caggaaatgt tcgcgatgaa aactggatcc gtcacccata ccagatcggt gctttgatgg
      481 acaaggaggg taagattaca aagattgaca agatcttgat tgaccagcca gcagactcta
      541 ctgaccactt ccgcgatcca caaattttta actttaaagg tcaatactat gctgtcgtcg
      601 gtggacagga cttggagaaa aaaggcttcg tccgtctcta caaggctgtc aataacgact
      661 acacaaactg gcaagcagtt ggcgaccttg actttgctaa cgaccgtact gcctacatga
      721 tggaatgtcc taatctggtc tttgtagagg aacatcctgt ccttctctac tgtccacaag
      781 gattggataa gaaagttcta gactacgata atatctttcc aaatatgtat aagattgggg
      841 cttcctttga ccctaaaaat gccaaaatgg tagatgtgtc tcaacttcaa aacatggatt
      901 acggtttcga agcctatgca actcaagcct tcaacgctcc tgatgggcgt gctctagcag
      961 ttagctggct tggtttgcca gatgtttctt acccatctga ccgttttgac caccaaggaa
     1021 ccttctcttt ggtcaaggaa ctcactatca aagacgacaa gctctaccag tatccagtcg
     1081 ctgctattaa ggaccttcgt gcttctgaag aagccttctc aaaccgttcc caaaccaaga
     1141 acacttacga acttgaactc aacttggaag ctaatagcca gagcgagatt gtcttacttg
     1201 ctgataaaga aggtaaggga ctttcaatca actttgacct tgtaaacggt caagtgacag
     1261 tggatcgtag ccaggctgga gaacagtatg cccaagaatt tgggacaact cgttcttgcc
     1321 ctatcgagaa tcaggctact actgctacaa tcttcatcga taactctgtc tttgaaattt
     1381 tcatcaataa aggagaaaaa gtattttctg gtcgtgtctt cccacatgcg gaccaaaatg
     1441 gtatcctgat caaatctgga aacccaactg gaatttacta tgaattagat tatggtcaca
     1501 aaactaactg atgtcgccaa acttgcaggc gtcagtccta ctaccgtttc tcgggttatc
     1561 aataaaaaag ggtatctatc tgagaaaacc atccaaaaag tcaatgaagc catgagagaa
     1621 ttgggctata aacccaacaa cctggctcgt agtctgcaag gaaaatcagc taagttaatc
     1681 ggcttgattt tccccaatat ttccaatgtt ttctatgcag aattgattga taaattggaa
     1741 caccaactct tcaaaaatgg ttacaagacc atcatctgca acagtgaaca tgattctgag
     1801 aaggaacgcg aatacatcga aatgttggaa gccaatcagg tagacggcat catttctggt
     1861 agtcacaacc taggaatcga agactacaat cgtgtgacag cgccgattat ttcctttgac
     1921 cgaaacctat cgccagacat ccctgttgtc tcctctgaca actatgctgg tggggttctt
     1981 gctgcccaaa ccttggtcaa gacaggtgcc cagtctatca tcatgattac agggaatgac
     2041 aattctaatt cgccaaccgg actgcgccac gctggttttg catccgtact cccaaaagct
     2101 cctattatca atgtttccag tgacttttct cccgtcagaa aagaaatgga aatcaagaat
     2161 atcttgaccc gggaaaaacc agatgccatt tttgcttcgg atgatttgac agctattctg
     2221 gtcattaaaa tcgctcaaga attgggcatt tctgtcccaa aagagctcaa ggtcatcggc
     2281 tatgatggga cctactttat cgaaaattac taccctcaat tggctactat caagcaacct
     2341 ttggaagaga ttgcttgtct cactattgat cttctcttgc aaaagattga aggcaaggaa
     2401 gtcgccacaa ctggttactt cttaccagtt acactattac caggaaaaag tatttaaaca
     2461 caagaaaact cagaccgatt cgtctgagtt tttatgatct taaattttcg agatagcgct
     2521 gggctgtctc taggttaaag gttttatctg agatgaggcg ctctactagg ggagcaactt
     2581 cagattcact agccccagct aggagagcta gggatttggc ctgtagtttc atgtgacctt
     2641 gctggatgcc cgtacttacc aaggctttga gggctgcaaa attttgagca agaccgatgg
     2701 acacgataat ctgggctaat tctctggcag aaggatttcc tagtagatca tgactgagag
     2761 ctacacgtgg gttgaggccg atagagccac ccttagtcgc tacaggcatg ggcagggtca
     2821 tctcaccgac caattcttct ctttcaaggt ccagcgtcca gcagctaaga ccttgatagc
     2881 gtccatctcg actggcaaag gcatgggccc cagcttcgat ggcacgccag tcattaccag
     2941 tggcaatcaa aatcgcatca ataccattaa aaattccttt attatgagta gcagctcggt
     3001 aaggatcagc ctgcgcaaac tgactagcca atgcaatttt ctccgcaatc tctcgtcctt
     3061 gatccttttg gcggctcaag tagcgaaagg ccatgcgaca gcttgcagtc accagagaat
     3121 cggtcgcgta gttggacagg attcccatga gactctgtcc ctgactgagt tcttctaaga
     3181 ctggtttcaa ggcttccagc atggtgttga gcatattggc acccatggct tcctgggtat
     3241 caacatgaat ataaacaacg agaaagtctg gttcaccttt tatctgctcg acatgcaggt
     3301 cactcgcccc acccccacgt ttaacgatag aaggataggc ttgattggca agctccaaga
     3361 gctccgcttt cttgctggca atcttctctt gcgctagttt aggattagca atttgataaa
     3421 gggctacctg cccaatcatc tggcgctgat ggacttgtgc agtaaaacca cctgcacgct
     3481 tgatgatttt gctggcatag ctggccgccg caaccacaga gggttcttct gtcacatagg
     3541 gaacggtgta ttcctgaccg ttgacaagta cctccggaac cagcgaataa ggcagagaaa
     3601 aagttcccac cacattctca ctcagctggt ctgccacagt cacactcatc tgttcatcct
     3661 tctccagact agcttgtctc tcaggactaa ggagcgcctg agcttttagc agctcgaggc
     3721 gctcttggta tgatttttta gaaaatccat tccaacttat cttcattatt tttcaacctt
     3781 gctataacgg cgttggtggt caagaatttc aaccaaggca aaatcttgat tttcatagcc
     3841 agcaaactgg gcagagtttg tttcatccaa gtttacttcc tcaaaaaaga ccttttcata
     3901 gtctgcaacg gatagggcag ttcgttggtt gagcttgttc aagcggtctt tatccaaata
     3961 agcttcatat ccttcaacca attcaccact gaagaactca gccacagctc cacttccgta
     4021 actataaagg gcgattttat ccccagcttt caagctatct gtattttcca agagagacaa
     4081 aagtccaagg aaaagtgaac ctgtgtagat attccccacc ttttgactgt agagaataga
     4141 ctggtcaaaa tgcttttgta agaggtcttt tttctcttga ggcaggctct tatccatgat
     4201 ttttttcaag ccttttagcg ctaatttagg ataaggcaag tggaaacaaa cagccgcaaa
     4261 atcatccaga gtaagctggt agcgtttttg atattcaagc caagtcgttt tcaaactatc
     4321 caagtattgt tgggtagaat aaacaccatt tacataagga gttgtcgagt aatttggtcg
     4381 ccagaaatcc atgatgtcac gggtctgagc tacattgtca ttattaaagg ccatcatgcg
     4441 tggattttgt gtaatcaaca tagctacact tccagcacct tgagttggtt ctcctggagt
     4501 ttcaataccg tatttggcaa tatcactggc aatgaccaag accttggact ccggagaatt
     4561 ttccacatgc aatttggcat aatgaagggc agcagtcgct ccataacagg cttctttaat
     4621 ctcgaaacta cgagcaaagg gctggatgcc cagcaagcca tgcacaaaga cggccgcagc
     4681 cttactctgg tcaattcctg actcggtagc cacaatgacc atgtcaactt cttgtctttc
     4741 ttgctcagtt aaaatagagt cactagcact ggccgccaag gtcacaatgt cctcagttag
     4801 gggcgcaata ctcaattcct tgagtaagag tcctttactt aatttttcag ggtcaattcc
     4861 cctcgcttct gctaagtctt gtaatttcaa gacatattga ctggtcgcaa aaccaatctt
     4921 atcaataccg attgtcatat ttacctctgt tttatcattc atgtaaaaaa tcgttctata
     4981 ctattttatc acaaatggca gtaaaagaga gaaaaaagac ttgattcacc aaatcaagcc
     5041 tcttattggt catcatttta aagaatgatt agttgctaga gagttcaccg atataagtag
     5101 ctttataagc tccattcaca gttatcagct cctggaggat caaatttcct gagtaagtcc
     5161 ttcccatctc atctacaaat ttttgataaa actgactggt cggaatttct ctgacatcct
     5221 tatcaaatgt cttatcaagt gttttactaa ccttctcagc aatcaattga tgctcttgcc
     5281 atccactttg aaactctgag cccgaactag aaaccatgac tgggataaac aacaaggtca
     5341 gtagatttac agacaataag gaaagtagta gacttcctgc aaaactagaa tcctagttca
     5401 tgattgataa taccagcaat caaattcatt cgtaatccga agcgtttacg atgatttcga
     5461 taggttgttg aaaacatttt aaacgttttt actttggcaa agatgttctc aaccttgctt
     5521 ctctccttag atagcgcatg gttacaggct ttatcttcag ctgttagcgg cttgagtttg
     5581 ctggatttac gtggagtttg tgcttgagga tatatcttca tgagcccttg ataatcactg
     5641 tcagccaaga ttttaccagc ttgtccgata tttctgcaac tcattttgaa caacttcata
     5701 tcatgactat agttcacagc gatatccaaa gaaacaattc tcccttgact tgtgacaatc
     5761 gcttgagcct tcatagcgtg aaatttcttt ttaccagaat aattcgctaa ttgtttttta
     5821 gggcgattga tttttacttc cgtcacatca atcattatcg tgtcctcaaa gctgagagga
     5881 gttcttgaaa tcgtaacacc actttgaaca agagttactt caacccattg gctccgacgg
     5941 attaagttgc tttcgtgaat accaaaatca gccgcaattt cttcataagt gcggtattct
     6001 cgcacatata gaaagcgtta tcaatttatt tatctcattt ttcagaaaat tcttttattt
     6061 ctgtaaagtc tacgatactc gatgtgtttt tatataatga tagagtctga gaatcactgt
     6121 tccgctagcc attccaatag agattaccaa agccaacatg acaaccaagg tcgcacttgc
     6181 cagtgcttta ttatagtccc ctgtcacaaa aaaggcagtt gttcggtagg agagataacc
     6241 tggaaccagc ggtgccaaaa tggccaagat aaagaccaca gcaggtgtct tataaagaat
     6301 acttaaaatc tggctgacac aagaaccaat aatggctgca atgaaggtag ctacaatgac
     6361 attggtcggt tccttgagca agagatagat tagccagaca gtcatgccca aaatccctcc
     6421 aggtaagagc atagaccgtt gcacattgag tacgattaaa aaagtgataa tggcaagaaa
     6481 acttgctact gcttgtaata aaaaggttgt tagtgtcata ttagttcatc aataccaagg
     6541 cgacagaagt tcctgcccct aaagcgaggg taatgagcag ggattcaaac atcttactca
     6601 taccagagtt tatgtggttg gtcataatat cacggaccgc attggtcaag gcaatacctg
     6661 gtacaaacgg catgaccgca ccagctataa tcaaatctgc cgttgaagga aaacctgtgt
     6721 agcgagccca aaactgggca attatcccaa agacaaaggc tccagcaaag gctgtcacaa
     6781 agggaattcg gataaatttt tccacataga gggaaaaggc aaaaccaaat aaggtcgcca
     6841 ctcctgcccc aagtgcgtcg tagatatttc cgctaaacat aactgaaaag aaaggagcac
     6901 taaaggtcgc agccagagtt acctgcaact tagtataggg aaggggtttg gcttgcaagg
     6961 ccgtcaattg cttaaaggct gtttctaagt caatctgccc cccaactagc tgacgagaaa
     7021 tctggttcac atcgcagact ttttcgatgt tataagaaga ggaggtcacg cgcttcatgc
     7081 gcgaaatatt ggtattttca atagagaaaa agatagcggc aggcatggca aggacattgc
     7141 aatccacaat cccctgcgaa tgcgcgattc gaatcatggt atcttctaca cgatggattt
     7201 ctgagccact tttaaggaga atagtccccg ctagcataat cacatcaatg acggcattta
     7261 attcttttga ttcttccatg ctttcctcct tttatcaact ccctctattc tatcacaaat
     7321 ccggactcaa aaaaaatctt tgccatgaaa tcatgacaaa gattgattac tcattttgat
     7381 tatccatctg cttttaagga gtagctgaag ttgttttagg tttgtagatt gaaatcttga
     7441 ctctagtctt atttaggtct accttttcac ctgctctagg actttgttca acaaccatgc
     7501 cttctacact acctgcaggc gctgtcgtca cttctacaac ttctatatta gcttccttaa
     7561 tcccaacaat ttgaatcaaa ttgttcttag taaactccaa gctggaacca atgtaactcg
     7621 gcatggcaac acttgtaact tttttagcta ctgtcaagac aatttgagta ggtttactca
     7681 catcataagt cgttccggca cctggacttt gtttcataat cgttcctggt tcgctttcgc
     7741 tggactcttc ttcctctatc ttaatcaaat tctcaggaac cttcttctgc ttgagttctg
     7801 agattacttc tgtagagttc cgtccaatat agttccctaa ttgaatcgtc gtagcttttt
     7861 tagctactgt caaaacaatt tgagttgcct tgctcaagtc ataggtcgta ccttctggta
     7921 gactttgctt caggaccgtt ccagcctcac tctcattgga ctcttcttcc tcaattttaa
     7981 tcaaattatc tggaactttt ttctctttta attccgcgat aacatcagaa gatttccggc
     8041 cgacataatt actaatttgg aaggattgtt tgcctgatga gacaaccaga ttgattttcg
     8101 ttccttcttt tcgaccagtt ccagcgccag gatctgtacg gataatccgc ccttcttcca
     8161 ccttttcact agcctctgtc ttctcctcac caatctcaaa attggctttt ttgagcgttg
     8221 ccttggcctc tgcaactgtc tgacctgcca catccggaat ggcaatggtt gcaggagttc
     8281 tggataagat ccaaataaga gaagctgcca ccaatacaag gctggccaac aaaatcaggt
     8341 aacgcatctt aaatctatgt tttttcggtg cttgtggttg gtaagtttcc tctgtcacag
     8401 cctggcttgg gtttttgatt gatttgtgtt ctgtctgcgc ttgaacctta ggaatagatg
     8461 tcaaggtact ctgagaaacc ttcggcaagg tcttggtatc tgccttgctc gtttcatcaa
     8521 agattaactt actttcattt ctacgattgt aggacaagct actagacaag tccacataca
     8581 tctctgaaac cgatcggtag cgattggtca actttttagc agttgccttg ataataacat
     8641 tttctaaagc ctgaggtaca gatggatttt ctgcaataac ggacggcagg ggtttctgga
     8701 aatgctggag ggcaatggtc accgcgctat ccccgtcata agggatatgg cctgtcaaca
     8761 tctcatagaa aataatcccc atggcataga tatcactctg cacagtcgcc ttagaaccac
     8821 gcgcctgctc tggtgacaag taatgaactg agcccaacat cgagttagtc tgggtcagac
     8881 ttgtctctgc aaaggctaca gcaatcccaa agtctgtgac cttggcagtc ccatctggtg
     8941 tcaaaaggat attttgaggt ttcaagtccc tgtgaacaat tcctcgagta tgggccaagc
     9001 gcatagccaa gagaatttgt cccatgatac ggactgcttc ttcattagaa agaggataat
     9061 gttccttgat atagcgtttg aggtccagtc cagccacata ctccattgca agatactgtt
     9121 gaccgtcttc ctcgccaata tctgttatcc gaacgatatg aggatggtct agatctgcca
     9181 tagctctcgc ttcacgctga aaacgagcta cagctatcgg gtccgtctgg tagttggtcc
     9241 tcagaacctt cactgccact tcttccccat ctaagattaa gtctttggct aggtagacat
     9301 ccgccatgcc tcctcgacca atctgtttga caatccgata gcgtccggca aaaatcttgc
     9361 cgatttggat cattctgcat cctcctcgtt catagaaaca agggcaaccg taatgttgtc
     9421 taaacctcct gcattgttag caaaacgaac aagtgtctcc gttttatctg ctaaaggaat
     9481 atcactggtt acaatatcac gaatctcact gcctgaaatc atgttggtca agccgtcact
     9541 attgagcaag agatagtcac ctgactcaag gataactgtc ccaaaatcag gctgaatttc
     9601 atctttttgc ccaatagact gggtgataat atttttttgc ggataagctt ctgcctcttc
     9661 tggtgtcaat tgaccagcct tgagcaattc attaaccaag gaatgatcgc tcgtcaactg
     9721 atggtattct tctccacgaa tcaagccgat acgcgaatca ccaatatgag catagatagc
     9781 ctgattatca ataatagcaa ggacttccaa agtagttccc atgcctctgt aagcttcatc
     9841 ctgaccaagc tggtgaatct tttgattttc aatttctagg taatgggcga accattcacg
     9901 cacttcattg actgtatcga tctgggtatc aacccaagct acacccaggt ctgtgaccgc
     9961 catttcacta gcgatattcc ctgcgcgatg acctcccatc ccatcagcta aaataatcat
    10021 ggtacgtcca gctctattga catagtggtt gacatagtct tggttatttg ttcgtttctg
    10081 accaacatct gttaataatg aaatttccat gtgtcagttc cttcctaatc cgatatcttg
    10141 cgaaattgac tgatgaagaa tccatcactt ccatacaatt caggtgtaat gaggatacag
    10201 ccgtctttca tgatatcctt acattcatgt tctagtttta cctgctcgaa ctcgggatga
    10261 ctctctaaaa aggccttaac gacttgaaaa ttctcctctg agacgatagt gcaggtgcta
    10321 taagttatta taccaccttt gcctagtatt tgacaaacac tacctaatat ttctaactga
    10381 atttcctgca aggacgcgaa atctgccgtt tctttattgt atttgatatc tggttttcgg
    10441 cgcaaaagac cgattcctga acaaggagca tccaccaaaa tcttatcaaa ggaatcctgg
    10501 tcaaaaaact catgcacctt tctggcatcc aatttttgag tttgaacccg atcttcaact
    10561 cccagacgtt gggcattttc ttgaattaaa tccaacttgt ggtcgtacaa gtccagagca
    10621 gtaacctgac ctgtcgtaag ataagaggct atatgggctg ttttcccacc tggagccgca
    10681 caggcatcaa gcactcgctc atcaccttgt aaatcaagcg tcggagcaac cagctgactg
    10741 gactcgtctt ggatggtaat ggctccatcc gcaaacaaat tatgccctgc aaaatgcccc
    10801 tgctccttaa ccagaccagt ggttgctaaa agggaatcat tcgcctccaa caaggcttgg
    10861 atttcctctt ttcgacttag gtctgttaca cgaatactgg ctttgtttcg cactaacagg
    10921 ctttcaaaga tggcttttgc tctctcctct ccgtattctt ccttgagttt ggcaactagc
    10981 caaactggga gagaataggc aatggagtca cgcttgtttt ttcgcttgat gctagcaata
    11041 tctggccagc cttcacgcaa gatacggcga aggagggcat tgaccaattt ttcactgcct
    11101 tttttacgaa gtttggccaa ttccactgct tcattgacca cagcatgatc tggaatcttg
    11161 tccaaatagc ggagttggta ggcactcatg agaagaagga catagagcca gctgtctaac
    11221 tggtctctgt cttcgataaa gtgggatagg taccattcca gagtcagttt acgggctacc
    11281 gttccataga ccagctcggt cactaagccc ttgtctgctg ccaaaagttg actccccttt
    11341 agatgcttat ttaaggcgat atttgagtat gcttggttca caaaaacatc ctctagcact
    11401 gctagagcta aacttctagc cgtttctact ttagtcacca aatcgttctc ctacagtcaa
    11461 tgtacgtcca actccgttga ggaaggaagc aatgtccatc ttaggcttac cagctggctg
    11521 cacttgtttg agggatagag ccccttcagc cgttgcgaca atcaattctt tcttgccgat
    11581 agagagaatc tcacctggat ttccctgacc ttctactggt agggcttcat aaatcttaaa
    11641 gcggtcgccc ttaaggaaag catgggcaac aggccagggg ttcattccac gaatttggtt
    11701 aaagagttga cgattggttt tgttccagtc cagtttttct tcctctggct ttatatttgg
    11761 agagaaggta acctgactcg tatcctgcgg ttcaggtttg atatcaccag caatataggc
    11821 aggcagagtg tccaaaagca aatcacgacc aactagcgcc aatttttcaa acaaggtgcc
    11881 aacattgtcc tcatctgtga tcggaatgct gcgacgagaa atcatatctc ctgcatccat
    11941 ttccttaacc atttccatga tggtcacacc agcttcctca tccccttgaa tcaaggcata
    12001 atggataggc gcaccaccac ggtgtctagg aaggagggag gcatgaacgt tgacagcaaa
    12061 gtccatgcta tcaaggagtt tgcttgggag aaactgccca aaagcagcag tcacaattcc
    12121 atctgctcct agcttcataa gttcttccat ctctggactt ccagataatt tttcaggttg
    12181 gtagatagat agtcctgctt ccttggcagc ctgcttgact ggggtttctt ggataacttt
    12241 tttacgacca acagcacggt ctggctgggt cacaacggct agaatttcgt aacggtcatc
    12301 tgtcaaaagt ccttttaaga ctgttgctga aaagtcgggg gtccccataa agattagttt
    12361 tgtcatatct tctccttctt ataaaaattg ctgcggctca tggtcaatgc tgagacggag
    12421 ctcactattt tcccgttctt gagtcaaggc caagacctgg ttgagggtcg gccccagctc
    12481 atcttctaaa cggtatttaa ttaaaatctg gtaatgatag aggttgtggg tacgggcaat
    12541 cggttttggc gttggcccca gaatgggact ggtctctgac aagcctgacc gcaaaatgtt
    12601 catgacttca taggcacgtt tgaaaacctc ttcttctttc ttgtgagaaa gggtaatacc
    12661 aatcgtgaaa tagtaaggtg gatagccgag ttgtcgtctg attcccattt cataggcata
    12721 aaagccttcg taatcttgat ccttagcaaa tcgaatagca tagtgctgcg aattgtagga
    12781 ctgtatcaag acttgacctg ctttttcagc ccgacctgct cgacctgcca cctgagtcaa
    12841 gagctggaag gttctctcag aagaacggaa atcaggcaga ttcaaggccg tatccgcatt
    12901 tagaactccg actagggtaa cattgggaaa atccaaaccc tttgcaatca tctgagtacc
    12961 aagtaaaata tccgcttccc ctcgcccaaa ctggtcaagc aaggcttggt gactgccttt
    13021 ctttcgagtc gtatccacat ccatcctcag aatgcgagct tggggaaaga gttctgctag
    13081 ctcatcataa gccttctgag ttcccgtccc atagtaacga atactgcggc tcttacagtt
    13141 aggacagacc tgaggaatat ccttcgagaa accacaataa tggcagttca tagtcttggt
    13201 atccatatgc aaggtcagag aaatatcgca gttgggacaa gtatccaccg tcccacactc
    13261 ccgacacatg acaaagctag aataaccacg gcgattgagc atgagaacca cctgctcttt
    13321 tttaaccaga cggtcttgga tagcctctag caaaggaggc gtaaagtttg acgtctcatt
    13381 ttgtccgata tagtctcgaa agtcaatcac ttgaacctca gggattgtag ccaaaggatt
    13441 ggcacgttgg gttagacgta agtgttgata gacgcctttg ccagcacgtg cccggctctc
    13501 taagctcggc gttgcagatc caagtaccag agttgcttga ttatactgag cccgtaaaat
    13561 agctacctct ctggcatggt aacggggatt gctgtcctgc ttataagccg cttcatgctc
    13621 ttcatcaata atcatgacac ccagattttt cagaggagca aagatagcag atctggcacc
    13681 aacaacaact tgggcatcgc cacgctccac cttgcgccat tcatcatact tttcgccatt
    13741 ggataatcct gagtgaagaa tggctacctt gtccccaaaa cgtgctataa aacgctcggt
    13801 catctgagga gtcaaggaaa tctcaggtac cagcaaaata gctgtcttac ccttatccag
    13861 ggcaccttgg ataatctgca agtaaacctc ggtcttccca cttcctgtaa tcccttgaag
    13921 tagaaaggga ggttgagaac tgccaataga actcacaacc gcatcacgcg cctgtctttg
    13981 ttctggattt aactccaaag gtctacttgc ttcaattcct tcaaaataag cagccgagcg
    14041 tcgaacttcc ttttggacta tggtaacagc accttgatcc acaaagaagt tgacttgctc
    14101 tcgcgagtag gactctaaca agctagccaa ggaagcgctc tctggatgag acagcagata
    14161 atctctcagt tccaactttt tcttggcacg tgtagaaatc tcaacacctt ctaattgagc
    14221 atggtcaacc tcataccaag actgggtctt gaccttcttt tgatcgactg cctgatattc
    14281 cagaccaagc aggccttttc tagtcaaacg catcatttca gcttgcttac ctaaatccag
    14341 agaagaaaag gctagcgaat cttctgaatc aaacaggcgt actcgttctt cctgactcaa
    14401 gccttccaga ggatagagaa tcttgtcata gctagaattc agaaatcccg gaagcatggc
    14461 cttgaggata gagattttgt aggagaagac agatttgcgt aactcctcag ccagccagag
    14521 ttgttctggc gtgagaacag gagaaaaatc cagcacctct gcaatatctt ttaaatcttg
    14581 ctccatctct tctccatctg attgggactc caaaccaaga acaatccctt gaatcaggcg
    14641 attaccctta ccaaaaggca catgaacccg catcccaact tccagcattc cctcaaattc
    14701 ctccggaatc ctgtaactat agggctggtc cgtctgcatc aagggcacat ctacgataat
    14761 cttagctagg gccatcttct cacctcctcc ttgtcagtac attcttgcaa tagaaaaaat
    14821 aagattgagt ccccccaacc ttaaattttt tcaccatctt ctttttcttt agcaatttgc
    14881 tctttgattt tcttttcttc ttcttctttg cggcgttttt cttcttcgat acggcgacgc
    14941 actgcttcac gttttccttc tggatctggg tgaattgtaa cgtttcctga ttcgatttct
    15001 tctaaagcgc gaagagttga tttttcagac ttgaaacctt gagttgctgg ggcacctgct
    15061 tccaattcgt gggcacgttt tgcttccaag attacgagtg aatattttga aggaaccttg
    15121 tcgagcaagg tatcaataga gggttttaac atcatttgct tgtacctatt ttctaaattt
    15181 tatcgggtag ttggagattt tggtaacatc tcctgatagt gaccaatgac acgatccaca
    15241 cagaagtgtt ctgcttcaat cacacatttg acacgttcag cagctagggg tacctgatcg
    15301 ttgacaatcg cataatcata ctcacgcatg agggcaattt cttccttggc cttttcgatt
    15361 cgttgggcaa tcacttctgc actatctgtt ccacgaccta ccaagcgatc ttgcaattca
    15421 tccaaatctg gtggtgtcag gaagataaag acagcatctg gaaccttttt cttgacctga
    15481 agagcaccct gaacttcaat ttcaaggaaa acatcgattc ccttgtccaa ggtttcattg
    15541 acataggtca gaggagttcc atagtagtta ccgacatatt ctgcgtattc caacatctgt
    15601 ccttgacgaa tcagctcttc aaattcttca cgagtacgga agaaatagtc aacaccgtcc
    15661 acttctccag gacgttgtgc gcgtgtcgtc atcgatacag aatattgaaa ttggttttca
    15721 gaactctcaa aaatctctct tctaaccgtt ccttttccaa cccctgaagg accagaaaaa
    15781 acgattagta agcctcggtc tgccattgtg tctcctttta gtcaatctgt gaaataacat
    15841 ttctctagaa taatggcaaa aagccagatt atcctttaca gtctttctat ctagtgtaac
    15901 aaaaaagcag taatttttca actgctcttt cttatttatt tagcataatc tactgcacga
    15961 agctcgcgaa tcacggttac cttgatattt cctggataat cgagattgtt ttcaattttc
    16021 ttacgaactt tgtgagccaa gattgtgact ttgtcgtcct tgatttttcc tggattgacc
    16081 atgatacgaa tttcacgtcc tgcttgaagg gcaaagctag tttgcactcc ttcaaagccg
    16141 ttagcaattt cttccaaatc atggagacgc ttgatgtagc tttcaagaga ctcactacga
    16201 gcacctggac gggctgcgct caaggcatct gctgcagcga cgataactgc tatcacgctc
    16261 tcagcttcaa catctccgtg gtgactagca atcgtattca ccacaactgg gggttccttg
    16321 tacttacggg ccaattccat accgatttca acgtggctac cttcaacctc atggtcaatg
    16381 gctttcccga tatcgtgaag gaatccagca cgacgggcaa gagccgcatt ttcaccaagt
    16441 tcgctcgcca tgataccagc caacttagca acctcaatcg aatggcgcaa aacattttgt
    16501 ccatatgaag tacggaactg caaacgtccc ataatcttca tcaagtctgg atgaaggttt
    16561 ggcgcaccaa tttcataggc agcagcctca ccgtattcac gaatcttatt gtcaatctct
    16621 tgacggtttt tctcaaccaa ctcttcgata cgagctggat gtatacgacc atctttgagc
    16681 aacatttcca tagtcatacg ggcaatctca cgacgaatcg gatcaaatcc tgacaaggtc
    16741 accacttctg gtgtatcgtc gataatcaca tcgacccctg tcaaactttc aaaggtacga
    16801 atgttacgac cttcacgacc aataatgcgt cccttcatag tatcgtctgg cagatgaact
    16861 gttgagtttg ttgactccgc tacatattca ccagcgatac gttgcatagc ttgaaccaag
    16921 atgtccttgg ccattttgtc agaacgttcc ttgacctctt gctcagcttc gcgaatgcga
    16981 ctggcaatct ccctggtcaa gttttcctct gtctgagcca agataatatc tcgtgcttct
    17041 gcctgagaca gagcaccaat acgctctagt tctgcttctt tttgtctttc gacttcctct
    17101 aattgctctt cacgcgcatc aaggtttttc gctctatcag aaatactttg ttctttttgt
    17161 tcaagtgttt gttctttact cgtcaaattg tcgtccttac ggtcaaggct agtagctctc
    17221 tctgtcaaac gactttcgat ttgtttgagt tcttgacgtt ctgatttgaa ttcagcgtcc
    17281 acttcttcac ggtattttct ggcttcttct ttggcctcca atagtgcttc ttttttaaga
    17341 gacttgcttt cacgtttggc ttcattaaca agtaaatccg cttcacgctc agcttgtcca
    17401 cgtaaattag ttgcttcttg ttcagcattt aaaagcatca actctgcagc ttcctgagat
    17461 gatttcatct tagctgagat gctgacatat ccaatgacta aaccaatgat gacggcaaaa
    17521 acagcaatcg caagcgacat gatttccatg tttttacctc attttattgt tattccgaat
    17581 gacatacatt cttttacatt ctaccataaa aaagtgattt tcacaaacct aaaatagaat
    17641 atgttttggg gaatttggaa cacatttacc aaaataaact tgttgtttag aaatagtagt
    17701 ttagtagaga cttgagaaaa agcctacctt tcaatagact tagtaatgat ctttaaagga
    17761 caagaaagcc acgctatctc catccatcat ataaatcaag cgattttctg catcaatacg
    17821 ccgtgaccag gctccttggt aatcatattt gagtggttct ggtttaccta ttcctgtaaa
    17881 gggatcacgt tgaatatcct tgattagttt attgattctt tttaacgttt tcttatcctg
    17941 attttgccag tagcaataat ctgcccaggc atcttctgta aacttgagca gcatttctta
    18001 ctcctcaata acatggacct gagtacttcc agcacgaact tgagccattc ctcgcaaaac
    18061 cttatcagaa agttccttat tttgagcaat tctcagggtt tcttggatac tatcccactc
    18121 actctttgaa aggactacaa tgtcctcatc tggattttta ttgaccaccg tcaaaggctc
    18181 aaattcatca tttaccttct tcatgtagtc ctttaaatga tttcggaatg ttgagtaaag
    18241 gactgcttcc ataaccatac ctcgttttag ctcttttcca ctattataca cgaaaagaaa
    18301 gaaattgtca ggaacttgta caagattttc ttttctatct atttatactc aatgaaaatc
    18361 aaagagcaaa ctaggaaact agccgcaggc tgtacttgag tacggcaagg cgacgttgac
    18421 gcgatttgaa tttgattttc gaagagtatt attcgtaaaa aatctcaaaa agcctacctt
    18481 tcggtagact tagtttgttt ctattctaat cggcactctt ccaaaattct ggtctgctat
    18541 acttggattt cctagttggt aaatctggtc tgctttttgg gtcatagcat cccaaggttc
    18601 tttgccaatt cggctgacta gactgacctg acctttcaaa gacttgagat gttgcctacc
    18661 tttttcagta aatccaagga catgaatgac ttctggcaag acattttctc tagcctgcat
    18721 caaaatatag gtcaataggc gtctgacacg cgccttggtg taacgtttgg tagtcactaa
    18781 ctcgaccaat tcttccacag actgggctgt cttgatggct tccttaatgc gtactaccat
    18841 ttcttgattg acctgataga cggtggttag gtcgggattt aacaaaattt gatatcggag
    18901 caaaggaaaa tagtcatccc agctcacctt actagcctgc tcaaagaggg caacagaagg
    18961 cataaagcgt tctaagaaat cttggtctga ctgatgctgg cgcagagctg tcgccgaggc
    19021 aaagtccaca tccttgtcca cagaatgata gccagccccc tgacgctgaa tcggatgcaa
    19081 cttgatgttt cgtcccgcaa ccgccttggc ataggccaga gcaagaacat ggttgggcgt
    19141 atttcctgaa aaatcaagac ctgcaaattc cttccacatg gcctgggttt tctggggata
    19201 ggaaagggaa tcaggcagat tttccacaaa actctccatc tcagcacctc gctctgtgta
    19261 taagtcaaca attttctggt aatccagaac ttcctccgtt ccaaaagcga ggctatctat
    19321 gcccaatcga gccaagatat ccacagctcc ttggccaaag aaatctgccg cctgaacact
    19381 gactaaaaag ggcaattcca ctaccaagtc tgcgccattt tccagtgcca tctgggccct
    19441 tgtccacttg tccacgatag caggctctcc acgctgcatg aaatttcctg acatggcaac
    19501 gattttcagc ccctcagcct gatccagcag gtatttgtgc ccattatgaa aaggattgaa
    19561 ctccgcaata atacctgtga tggtcatgct aatctcctac ttctgcgcga caaaaaacca
    19621 acgggtactt gtctctgtcg gttccttgtc ctcaaagtcc gcatagagtt tgaaggattt
    19681 gaatccagcc tgttccagca aaatatcata ggtcaagacc tcataggtcc gctcctcatg
    19741 cacttcatcg tggcgactaa aggaaccgtc agcctccttg ataaagaaag tcaactcatg
    19801 cacgatggag tgaggagctt cgtcctcata tgtatcccag agcatggcaa aatcttccgc
    19861 attttcatgg taagaataac ctgggaagac ctcatctgtc tggtaagtcg aatgcacatc
    19921 aaagatgaag acaccatctt cgttgagcac attatacact tccttaaaaa catccccgac
    19981 ttctacttca tcctgcatat agcagatcga gtccgagtaa cacgtgacaa aatcatattg
    20041 acctgacttg gacaaatcca gcatattgcc ttcaataaaa tcaatctttt gtttggctga
    20101 agaagctctc ttctcggcaa tcttcaacat atccccactc aaatcaagtc cagtcacatc
    20161 aaaaccagct tgagaaaagc gtacagactg aatacctgtc ccacaagcca attctaagag
    20221 tttctttctc tccttggtct taggcaagtg acgcagagaa aaatccgtcc atttatcata
    20281 taaactatcg tccatgactg catcatagac cgccgcaaag gtttcataag ttgccataat
    20341 catgatacca agagcctcca tggcaaacac cactcgctct ttacctttcc atcaattttc
    20401 taaaataagc aaagaaaccc agttgactac aactgagttc atcttctaag caagagcttc
    20461 tgaaatatct actgaatccg cctcatgcca tagcttctct agattataat gggcacgcat
    20521 ttcttctgaa aagatatgca cgacaacagc accgaggtcc agtaagaccc agcctccagc
    20581 tgcatcgcct tcgacatggc tacctttaaa gcccgcttga gctacttttt cacggatatt
    20641 agctgcgata gcgtccaact gacggctatt cattgagcta gtgatgacaa agtagtccgt
    20701 cacactagtc aaatcttgta catcaagtgc gaggatatcc tccgcacgtt tctcatcagc
    20761 cgctttcacg actagttcta gtaattcttt ttcgttcatt taatcctctt tttagaaaat
    20821 atgtttgtaa tctaggacat ctgcaaagcg ctcctcccaa atgtcctcat aaaattcatt
    20881 aatcgtttct gctggaatgt catccccatc aaaggttgtg acagcacctt ctggaacaat
    20941 aagctgatag ccatattcaa aggcaacctt gacagaagta tccacacaat attctgtctg
    21001 cataccacat aaaactaatt tttcaatccc ctgtttatcc aagtattctt ttaagccagt
    21061 ttctttgaaa atactgttat acttcttctg aaagaccttt tcagcaggtt ttcgatttaa
    21121 aagcgcagat aactgccaat cttctgatgt ttgagcttca gagttctcaa tatgttgaac
    21181 atagataatt tcaatattct tgcttctagc ctggttttgt aaataagaaa tttttcccaa
    21241 tagacttttt gtctggaacc cagtttctac taaaacattc tgaacatcaa tgattataaa
    21301 agccgtcttc atttagttct ctttcaaata gtgcacaaag gcgttatagg tttcaagggt
    21361 ttggggatag atggggaatc cctgatgagc tagatactcc acggtacgag ctgtttcgta
    21421 ggccaccgcc ttattgagcg atagacttgc aatctcacgc gccacatcta ctccaggaaa
    21481 ggctcgattg tgctcgatat agtctgcgac gtagatgacc ttatctaggt ctgtcatttg
    21541 accagcaccg actgtatgga cttcaatggc tcgcaggatt tctgaatcat gcaaatccaa
    21601 atcttcctga attttgtaaa ttccgaccat accatgccag acattattac cccagttttt
    21661 gaggtcaggg tctagctggt aacggtcaat caagtctaga aattcctgat ctgacaactt
    21721 tttagcatag tcatgaagaa gacctgctag acttgctttc tcaacatcta caccaaagcg
    21781 ctgagctaac tccatagcgg cacgctccac acctagacaa tgggttagac gtttctcagg
    21841 tagaagctct gccatttttt ccaacaaggc ctcacgggag cagttgatat agtcttgata
    21901 ggacatcagt agagcccctc cttctcgatg tagtctagca ctggctgagg taggagaaag
    21961 ttgggtttcc gaccttgggc aatgaagtcc cgcaccatgc tggacgagat atccatgagc
    22021 ggtacgtcca cccagataac tggataggaa gtccctgcct tgtagcgtgg acgctgaacc
    22081 cccacaaact gaaccatgtc aaccagttca tcaattcggt accacttagg cagatagtca
    22141 accatgtcgg caccgatgat aaagtaataa tccgtatctg gattcttctc tgtcaaaatc
    22201 ttcatggtgt cgtaggtgta ggaaataccc ttgcgctcca actcaatggt ttcaatgact
    22261 aggccgtcaa ttccctcaat tgccaactca agcatcttga gacgatggtg ttcagggatg
    22321 gtttcctttt tatcaacgtg aggaggttgg tattcaggca tgagaagaac ttgatccagt
    22381 cccaactgtt gccgtacttg atccgcaaca atgagatggg cattgtgaac agggttaaaa
    22441 ttccccccta aaatcccaac ttgtttgcgt tttttctcct tgatttctgg ctccaactct
    22501 accttggtaa agggagtcaa tagttcgatt gtcataggct agtccccttt atttctctat
    22561 aaaaacagtt atttggagtt tcggtcttag atttctttga ctttcttaga aatcttgcga
    22621 ttttctttct tgctagattg tttaaacaaa atcaagatgc gtcctatttt ttggactgta
    22681 tccacaccga tttcttcttc caaaatttca gctacttcgt ggatgttttc atctgtgttt
    22741 tgtaagagag taaccttgat taattcacgc gcatcaagag cttgacggac gctggttttg
    22801 atttggtcgt tgagtccatt tttcccgatt tggatgatag gtttgagggt gtgtgcctgg
    22861 ctgttgagga aggcacgttg ttttgatgtt aatgacataa tttctttaaa agagtttctt
    22921 tttatacttt tctgaagtgg tgacggacgt cagcaaagtc cttcggactt tcatgactaa
    22981 aatttgagcc taaggtctca aattttccgc agttggtaca atcacttgta ccaacttaca
    23041 ccacagcgaa aagtattctc tatggggctc gccttgctcg cccaaattca gacaaccctt
    23101 tcctttctgt gcttaaataa ttgcttttcg tgtgacgacg gcgacgcctt ctggtgccca
    23161 gacggcgact tttgctatgc ctgttacacg aatccaacct aggcctgaga tgactaggtc
    23221 tgtcttatct ttaatggtaa agacatgctg gactagcctt gggaaatctt ctttttcctt
    23281 gctatttggt ggtgtcagaa gagttcccag gtgcttatcg tagaaagcac tagctccttc
    23341 aagcttgcta cgatggagtt tgagttcatt atcaaagaaa gcagtaaatc cttgcttttc
    23401 tcctgctata aagtcaaagc gtcccaaacc acctaaaaat agggtttgct caggattaag
    23461 ctgataggtc ttaggcttga tttccttttt aggactgaca tacttgaggt ttttggccgt
    23521 caagtagtga gccatctggt ggcggtggat aattcccggc gtatcgtaaa tataagatcc
    23581 gtcgtcaagc ggaatctcta ttttgtccaa ggttgtccct gggaagcgtg aagtagtgat
    23641 gacattctga tcacccgtga tttcttggat aatagcattg attagagttg attttccaac
    23701 gttggtcaca ccgaccacat agacatcgcg gcccttacgg tagtgttcaa tcttgtcaat
    23761 gacttcctta atggcatgtt tattttgtgc tgaagttagg accacatcga ctggacgaag
    23821 accttcttca tgggcacgtt tcatgagcca ctggctaatc ttaccagact taactgactt
    23881 agggaggata tcttttttat ttcctaccaa gaggacatca ttgcccgaga cgaaacgtgg
    23941 taaacctggg atgacagagc cattaaaatc aaagatatca atgacattga ccactaaggc
    24001 atcactgtct cccacctcgt gcaagagctt gaggaaatca tcgttcgtca actggacatc
    24061 tgtgatttca ttgtagtggc ggagacggaa acagcgttgg caatagactt cgccagtctc
    24121 caaacctttt tcaagtgccg actggggggt aaaaccaaga ccagctttat ctgtcgtctg
    24181 aatggttgct ccacaaccaa tacagagaat ttcttccata gttaaattcc ttttttatat
    24241 gtaatcggtc cgtacttttc agtgattttt ctcataacac gacgctcacg agttcggtta
    24301 atctgcgttt tgattgagtc atgttggacc aagggtttga ctaaaattga ccgaatccct
    24361 gcacggtggg ctgctcgtat atctgtcatg agctggtcgc caaccatgac cacttccttt
    24421 ttgtcatagt ggaattcctt catggcacgg tcaataccaa atgtgaaggg cttcaaggcc
    24481 cagtaaacgt aatcaatccc aaatttctca actgctcgtt gaacgcgttt tttggtgtta
    24541 tttgacacta cgataatgcc aatacccgcg tcccgaaggt catgtagcca ttgcttcatc
    24601 tctggcgttc catcagggtt gttccaagca atgagggtat tatccaaatc gaccaaaaca
    24661 gccttgattc cctgcgcctg caggcttggg actgtcagat catagactgc ttccacagca
    24721 aaatctggta tataattttc aatcgccatt ctggctcctt ttcttaactt tttttcgcaa
    24781 ttttccttag taacaaggat aaaacaatcc acaaacctgc actagccaaa ctgatataga
    24841 gccaagcatg gggctcatct gttaaaggta agggaacatt cattccgaaa aagcctgtca
    24901 cgactgccaa aacagctagc aagactgaaa tgatagtcaa ggttgtcaaa ttgtcattca
    24961 gattattgtt tagaatattg ttgtaagagg ctgaaagctg ctgtaaaatc tgagagatta
    25021 ggtctgtcat ggataccagc tgatgagcct caatcatagc atcatcaaac tgttctctct
    25081 caatctcatc aaaactacga tacaaggcat gaccttgaat atgctctaac aaaatccgat
    25141 tttgtttggc agctgccgtc agataaacca taccagtctc caaatcagac agggcaaaga
    25201 ggtttttctt ggtagttcgc tggcgcaaga ggtcattgac ctcatcccta ctcttgtcca
    25261 tctgctcaat gacaggatag taggcattgc tgatgatttc cagactggca aagagaaact
    25321 tataaatcga aagcgtgtca tggctctcca gataacgagt catctgttca atgacatagg
    25381 cgttcttggt attactaatg gtaatcaggc gacgatgctc gacgataaag gtcatgggaa
    25441 aagtctcata gtaggccttg tcctttttta cgtctaggac attatagata aaggtaaccg
    25501 tcccactttc acggtggtag tccatgtggg cgcgctcgtt tctatccagt gcgtattcaa
    25561 tggtttcctt gtccaaaccg taaatttcag aaaggtcttc tagtttattc aactttccta
    25621 ggtctaggtc tatccaggta caaccattgc ccaactgctt ttctaaaacc atcttttctc
    25681 cttattaaac tccctctatt gtaccacaaa ttacttaaaa taacaggtct agtctgtgcg
    25741 tgagaaattg ctaacaactg tgatatttcg gttagggaca aagtgaaggg cttgctcctc
    25801 gctacgcagc tgtgtcgtac gaattcccac actgacaacc ttacccgata cagtaatcgg
    25861 tccatttgtc agaacgacct catctcccac atccagttga cgttcaaaga ggatgaaaaa
    25921 gccattgatg acatcagaca gaaagccttg ggctcccata ccaatcgcta ccccagcaat
    25981 accagctcca gccagcaaac tagaaactgg caaacctaaa atagacaaaa tgcagtagag
    26041 taaaaagaaa tatagcgtat aattaaacac attttctagt aaacgtgaga tggttttttg
    26101 gcgtccgaca tcatgacgag acatttttag agaaggtttg acaattctct gcaccatggt
    26161 atgaagcatt tttttagcaa tataaaatac aatcaaaagc agtaaaagag aaatgacctt
    26221 ggttagaata ttctcgataa tggttgtcac atctagcttt tcaatataag cctgaataaa
    26281 tttttgcata taaaactcct ttatttcatt ataccatatt ttcatcagtc tcattctcca
    26341 taaatattca aaaaatagac agaaaattct tgattttgtc tatcatttat actataatca
    26401 taatcactac acaaaaagga gattattatg aaaagaatca ttcatgcttg gaataaggca
    26461 agccttgtca agcgtatctt gattggtatg ctttgtgggg aatcctagga ctgacccttc
    26521 ctaatctctc agggattggg ctactcgggg atttattcgt tggaggtcta aaagctgttg
    26581 ctcctatcct agtctttgcc ctcgttgcca atgccctttc ccaacatcaa aagggacaag
    26641 atagcaatat gaaaactgtt gttttcttgt atatcctatg atagggactt tcgccgctgc
    26701 tcttgtagct gtactagcaa gtttcatcgt ccctattgaa attaccctaa atagtgccaa
    26761 tactgaaatt gcaccaccag atggggttgg gcaggttctc agcaacctct tgctcaaact
    26821 ggttgacagc ccagtcaacg ccctgtttac tgctaactat attggaatct tatcttgggc
    26881 agtcattttt ggaatcgcta tgagagaagc cagtaaaaat agtaaagaat tgctaaaaac
    26941 tatcgctgac gtgacttcta aaattgtcga atggatcatc aatctggctc catttggaag
    27001 ccttggtctt gtttttaaaa ccatttctga caagggagtc ggaagccttg ccaactacgg
    27061 tattttattg gttctattag taacgactat gctttttgtt gcccttgtgg tcaccctttg
    27121 attgccttct tctttatgag acgtaatcct taccctctag tttggaactg cctccgtgtc
    27181 agcggtgtga cagccttttt cactcgtagt tctgcggcta acattcctgt caacatgaaa
    27241 ctctgccatg accttggact caacccagat acctattctg tttctatccc actcggttct
    27301 actatcaata tggctggagt agcgattacc attaacgttt tgacccttgc tgcagttaac
    27361 actcttggaa ttcctgttga ctttgccaca gcctttgtcc tcagtgtggt agcagctatc
    27421 tcagcctgtg gtgcttcagg tattgccgga ggttccctcc ttcttatccc agttgcttgt
    27481 agccttttcg gtatttctaa cgatattgcc atacaagttg ttggggttgg ttttgtgatt
    27541 ggtgtcatcc aagactcatg tgaaacagcc cttaactctt ctacagatgt cctctttacc
    27601 gccgttgccg aatacgcagc aacccgtaaa aaataactca tcaaggcaag cctgctcatg
    27661 tcttgtcttt tacgctttta ttctaactta ttaggaaatt cttatgtcta ttagccaacg
    27721 tacgaccaag ctcatcttag ctacctgtct tgcctgcctg cttgcttatt ttctcaatct
    27781 ttcatcagca gtttcggctg gaattatcgc tctcttgagc ctatctgata cgcgtagaag
    27841 tactttaaaa ctggctcgca atcgtctttt ttctatgctt ctagctctgg ctatcggtgt
    27901 tctagctttt cacttgagcg gatttcatat ctggagtctc ggcctctatc tggccttcta
    27961 cgttccttta gcctacaaga tgggctggga aattggcatc acaccaagca ctgttttggt
    28021 tagccatctc ttggttcaag agtcaacctc tccagacctt ctagtcaatg aattccttct
    28081 ctttgctatt ggtacaggat ttgccttgct tgttaatctc tatatgcctt cacgagaaga
    28141 ggaaatccag cactaccaca cgctggtgga agaaaagtta aaagatatcc tccagcgctt
    28201 caaatactat ttatccagag gagacggacg caaccgagca cagctggtag cagaattaga
    28261 cacgcttttg aaagaagccc tcagactggt ctatttggat cactctgacc acctctttca
    28321 ccagaccgac taccatatcc actactttga gatgagacaa cgacaaagtc gtatcctgag
    28381 aaacatggcc caacagatta acacttgtca ccttgccgcc agtgaaagcc tgatcttagc
    28441 gcaactcttt tcaaaaattg caggtcaact gagccagacc aatcctgctt ctgatttgct
    28501 agatgaaatt gaacgttatc tggaagtctt ccggaaccgc agtctgccca agacaagaga
    28561 agagtttgaa acccgcgcca cccttcttca actcctacgt gaagccaaaa ccttcatcca
    28621 agtaaaagtt gatttttacc aaaaatatgg acagtaaaaa ataaaaccac taaaaaacag
    28681 agcttgccag cattcttgaa aagtagtata attattgcat gcgcaatcat cttgtgacgc
    28741 agagactgcc cgttaatgga ctttcttcta ttttatgact ctaaaaagaa aggagacact
    28801 atgacctatt tggaaaaatg gtttgacttc aatcggcgtc agaaagaaat tgaaagtctc
    28861 ttggaagaga ccattgccca acagagcgag caaagtctga ccttgaaaga gttctacctg
    28921 ctctactatc tggacttggc tgaagaaaaa tctctacgcc agattgacct gccagataag
    28981 cttcatctga gcccgagcgc agtttctagg atggtggctc gcttggaagc taaaaattgc
    29041 ggtctactca gtcgcatatg ttgccatcaa gatagacgct ctagttttat ctgcctgaca
    29101 aatgatgggc aaaagacact ggcctcgcta caaaagactg tcgaagaaag cttggaagca
    29161 ggtttggatt tcttgattta agatgatttt aaaaaaagtt gcgtgcgcaa tcatttttct
    29221 tgacattctc ttttataagg agtaaaataa agttagcatt aaacaaagga gttttaaata
    29281 tgattgaaat tacctatata gatgccagca agaacgaaag aactgtgact ttcgagtctt
    29341 atgaagactt tgaacgttcg caacaagctt gccttatcgg cgtcgcagac tactaccctg
    29401 tccaaaaatt aacttacaag ggccataatt tggactacca tgggacttat ggagatatct
    29461 tcttctatct catgaaacaa gatttaagcc aatataacta aaaaaaggag aaatacaatg
    29521 gcaaaagcaa ttacagatgc aacattcgaa caagaaacaa aagacggttt ggtcttagta
    29581 gacttctggg caacttggtg tggtccatgt cgtatgcaag gtccaatctt ggacaaattg
    29641 tctgaagaac tttcagaaga tgtcttgaaa atcgttaaaa tggacgttga tgaaaatcca
    29701 aacacagctc gtgcttttgg aatcatgtct attccaactc ttctcttcaa aaaagacggc
    29761 caagttgtca aacaggttgc aggtgttcac acagcagaac aaatcaaggc catcattgct
    29821 gaattgagct aatcaaacga gagaccaagt actttctttg gtctcttttt tcttgccctt
    29881 tgccattttt caaaaaatat gctagactat agcagattga aactagaata gtacacctct
    29941 acttctaaaa cattgttaga aatcgatttg actgtcctga tcgatttgtc ctgttcttat
    30001 ttcattttac tatataagta gaatattacg atgtttggga tatgccatcg ctaaaaaaac
    30061 tcgatttggt attttttagc tccctaatgg gagctttttg catgctctga acatttccca
    30121 ttttggaagg agtactatga aacgtcaatc agccttggtc gtctttagtg gtggtcaaga
    30181 ttcaacaacc tgccttttct gggccatgca acactatgaa acagtcgaag ctgtcacctt
    30241 tgcctacggc caacgtcatc acctcgaaat tcaaattaat agagaaatcg ctaaggaaca
    30301 gggcattcgt caccatatcc tcgatatgtc tctgctggga caaatcactg ctcagccaga
    30361 ctttgcgacg attcatattt cctacattcc tgacaagctc tgtgtcgagt caaaatccct
    30421 caaactatat ctatttagct accgaaacca cggagatttc cacgaaaact gtatcaacac
    30481 catcgggaaa gacttggtca acttgctaga ccctcgctat ttagaagtct gggggaaatt
    30541 cactccgcgc ggtggcattt caatcgaccc ctactacaac tacggtaagc aaggaactaa
    30601 gtatgagggc ttggcagaac aacgcctctt ccaacacgac ctttatccag agaaaattga
    30661 caaccgctaa agtcatactc aatgaaaatc aaagagcaaa ctaggaagtt agccgcaggt
    30721 tactcaaagc agtgtttgag gttgtagata gaactgacga agtcagctta aaacactgtt
    30781 ttgaggttgc agatagaact gacgaagtca gtaaccatac ctacggcaag gtgaagctga
    30841 cgtggtttga agagattttc gaagagtatc atacgaaaaa gccctgttcc tcaagatgag
    30901 gagcaaggct ttttgagttt caattattct tctgttccaa ggaaattttt tgcaacaagg
    30961 gctgcaagaa ctccaccagc gattggtgca aggatgaaaa tccaaacttg ttgaagggct
    31021 gcgccgccta ccaagacagc tggtgccaag ctacgagctg ggtttactga aagtccagta
    31081 atgttcaatc cgacaaggat catcgccatc aaggacaaac cgattatcaa accagcaatc
    31141 gcgccatttc ctttactttc tgaagtcaca gtcatgataa ccaaaacaaa caagaaagtt
    31201 gcgatgactt caaacaagaa accaccaaag acagtgacac cgtttgccaa agcattttca
    31261 ccaagactag cagttgacat acctgaattt gccaagagca agaagacagc gccagaagca
    31321 ataaaagctc cgacaacttg tccaaggatg tagtttacaa ggtcttttga tgacaaacgc
    31381 ttgttaacaa acatagcaat cgaaacagct gggttcaagt gagcacctga aacagttcca
    31441 attgagaaag ctgcgactac gattgccaaa ccaaaggcaa aagcaattcc aaggtgacca
    31501 aggccatcaa aaccatttcc aaaaacaaca gctcctgttc cgatgaacac aagcatgaac
    31561 gtaccgatta actcagcaac aaattttttc attttctttc tccttttttc aaaaactaga
    31621 tactagtcta tcaaaagagg gaaagggttt caagaaaatt gcttggaaag ttttttgaaa
    31681 atctgactcg ctttttctta gtattgaaaa gactggatag cttctttcaa gtcatcttgt
    31741 aaactatttc tctggtcaag ttgaacatag acttccaaca gacaggatct gaagttggaa
    31801 aatttataaa aatcttctct ttcttctatc ggaaagtcaa cagcttttat ccaagaagct
    31861 acttgctctt gcgctaactt cccttgtaaa ataggttcat agatcactct tgctaaacgc
    31921 caatcctcat catctgtaaa gcgaatcgaa actcttttaa atagttggcc aagtatatca
    31981 aatacttcat gaactctgtt tttaggaaag ttgggatgac aaaccacctc tgtcagtaaa
    32041 tcggctccat gtgcaaaagc atgaacccaa ccatactgac ttgaaaaacc cgttgtatcc
    32101 ttttcttttg aaagataata caaaccttga tttaaaagga cattacgaat tcctgcattt
    32161 aattcctgat aaaaatcgat tgctggttgg catctgcaga caagagattt gcataaataa
    32221 gcgccctaaa agaacgttca agggttgaca ggcctacctt atcaatatct ttatctaacc
    32281 ctccatcaga tgaaatccct tcagcaatga aatgaaattg ttcctgtgta aatagctctt
    32341 cctgaatccc tctagcaaag cttgtaaaaa caaggtcatc acgaatttct ggagaaggat
    32401 ctcccaaatg atcaagtagc cactggattt cttcctggtt ataacttggt ttttcttctg
    32461 ctatttttct aagtaacttt tgatacatgg tcaatacctc tacatttcta gcaactgttc
    32521 aaaaagctgt ccttaaatga ctttagcaat ttgtaaaatt aagttaaaga gaaaaagaca
    32581 tttatattca gagaaacagc acatttctga aaggatacat aaatgattta ttccatatta
    32641 ccacaaatga acttgtaatg atagaacttt atcacaaaaa tcacatttct gtctcaaaaa
    32701 ttgcgaaaaa ctagttataa taaagtgaaa taaaaactat acaaattgat tagggaattt
    32761 aaaacaattt ctaacaatat tttaaaaact gaaccgtact atttcaactt ccataaatta
    32821 caatattgat aggcacagtt cccatacctg tccaaattcg aataacgttt agtataaatt
    32881 agaatatgac tacaacatca caaataatta gactatctat aatatctacg atgtaacata
    32941 tacataggaa gcaccgttat caagagcgcc aaggctatca accagataag atgaagatac
    33001 aaacctgtca cctcacctcc ccatataata cgaatcatgc gggtagcgtg atacaaggga
    33061 gaaaaccaag tcaaatcacc aagtattggg ggcaaggaag taatgggaaa gaaggtatct
    33121 gaaaacatat aaagaggacg gattaccaaa acattgtagt aattcagaaa ttccctagac
    33181 ggtgccaagt aggtaaagat tagtcctaaa acaccaaaca aataacccac tataaataaa
    33241 atcagaggaa tcaataacat caagacatga aaatgcgctg tgataagcat ggaaatcagc
    33301 caaaaaatcc caccgaaaat cactgcacga atacctgccc agatagcctg acccaccaca
    33361 atatcactcg tattgattgg cgtcatcaac atagcatcat aagtcttttc aatcttatac
    33421 tggataaagg cattgaccgt tgtttctgcg gtggccgcag acatggcagt cgcagcaatt
    33481 aaacctgtaa tgacaaaatc acggtagagc ataccgttaa catgcgtcaa gtaggcacca
    33541 actccaaatc ccattgctaa aagataaaag agggggtcga taaggttggg gacaattgaa
    33601 attttaaata aatttctaaa actcatgtag ttgcgtacca caacgtgtcc gatggactga
    33661 aatgataact taaacatgca agtcctcctt ctctgtccca gtcaacccca aaaaaagcgt
    33721 ctcaaggttg tcagcatcat acttgatgag caagtcttct acagttcctt cttctttgat
    33781 agtcccttgg tggagcatca gcaccctatc tgtcaaatat tcaatctcat tcatatagtg
    33841 ggaagtaata atgatagaaa cacctaattc cttcaactcc aaaagtttat cccaaataat
    33901 attgcgggat tgaatgtcta aaccaactgt cggttcatct aaaatgataa tatcaggccg
    33961 attgatgaga gctcttgcca agacaagacg tcgtcgcatc ccacccgaca gctgattcac
    34021 catcttttgc gcatgtttgc tcagttgaac aaaatccaag agagaatcga ttctcttcct
    34081 aagttcgaca gatgagatac cataacatag accgtgagca aaaagattat cataaacatt
    34141 caaggtcata tccagcatat cctcctgccc tacaataccc agacaagatt tactcttacg
    34201 acgattggtt tcaatatttt gccccaagat atcaatctct cctgaggaaa ttgaggtcag
    34261 tgagtaaatc atctttaaag tggatgtttt gccagcaccg ttgattccca agatacccaa
    34321 acactctcct tctcccaatt caaagctaat tcccttgaca gcttcataag aaccatattg
    34381 cttttttaga tttttaacac ttaacttctt cactctttct ttcccccaca aatagagcat
    34441 gttcgatact ttgttaattc atctcacttt tgaaaaaatg ttgagaatgt aatcttaaga
    34501 ttaacgtcct tcaactgtgg atactgctgc aattactttt gtaattttac ctcca
//
LOCUS       DAVMGZ010000008        34388 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000008 DAVMGZ010000000
VERSION     DAVMGZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.008"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            563..1015
                     /locus_tag="VYQ93_000311"
     CDS             563..1015
                     /locus_tag="VYQ93_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV3335062.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFIYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1034..2371
                     /locus_tag="VYQ93_000312"
     CDS             1034..2371
                     /locus_tag="VYQ93_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV3335063.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQASMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2450..3334
                     /locus_tag="VYQ93_000313"
     CDS             2450..3334
                     /locus_tag="VYQ93_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3335064.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTIFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3334..4167
                     /locus_tag="VYQ93_000314"
     CDS             3334..4167
                     /locus_tag="VYQ93_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3335065.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4185..6278
                     /gene="nanB"
                     /locus_tag="VYQ93_000315"
     CDS             4185..6278
                     /gene="nanB"
                     /locus_tag="VYQ93_000315"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV3335066.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPALIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGDSQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRVESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWRYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6290..7393
                     /locus_tag="VYQ93_000316"
     CDS             6290..7393
                     /locus_tag="VYQ93_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV3335067.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVRLSEIVK"
     gene            7567..8265
                     /locus_tag="VYQ93_000317"
     CDS             7567..8265
                     /locus_tag="VYQ93_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV3335068.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8284..9801
                     /locus_tag="VYQ93_000318"
     CDS             8284..9801
                     /locus_tag="VYQ93_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV3335069.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9926..11254
                     /locus_tag="VYQ93_000319"
     CDS             9926..11254
                     /locus_tag="VYQ93_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3335070.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11506..12372
                     /locus_tag="VYQ93_000320"
     CDS             11506..12372
                     /locus_tag="VYQ93_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3335071.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12388..13227
                     /locus_tag="VYQ93_000321"
     CDS             12388..13227
                     /locus_tag="VYQ93_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3335072.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13500..13952
                     /locus_tag="VYQ93_000322"
     CDS             13500..13952
                     /locus_tag="VYQ93_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV3335073.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            14049..14393
                     /locus_tag="VYQ93_000323"
     CDS             14049..14393
                     /locus_tag="VYQ93_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335074.1"
                     /translation="MVSSKHAARASFLDGQGITVDEMSWIINGIVNALIGRYIKLGTY
                     AAKYGISIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            14677..15321
                     /locus_tag="VYQ93_000324"
     CDS             14677..15321
                     /locus_tag="VYQ93_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV3335075.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLLELGIVSLSLLDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15490..16407
                     /locus_tag="VYQ93_000325"
     CDS             15490..16407
                     /locus_tag="VYQ93_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV3335076.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLDTARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16425..17309
                     /locus_tag="VYQ93_000326"
     CDS             16425..17309
                     /locus_tag="VYQ93_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV3335077.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17325..18176)
                     /locus_tag="VYQ93_000327"
     CDS             complement(17325..18176)
                     /locus_tag="VYQ93_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV3335078.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18376..20433
                     /gene="pbp2b"
                     /locus_tag="VYQ93_000328"
     CDS             18376..20433
                     /gene="pbp2b"
                     /locus_tag="VYQ93_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV3335079.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKVTTSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVPTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELGNGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTAFSRPMPITAVQALEYSSN
                     AYMVQTALGLMGQTYQPNMFVGTSNLESAMGKLRSTFGEYGLGSATGIDLPDESTGFI
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANDGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSVLHQGFYQVAHGTSGLTTGRAFSNGALISISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20444..21040
                     /gene="recR"
                     /locus_tag="VYQ93_000329"
     CDS             20444..21040
                     /gene="recR"
                     /locus_tag="VYQ93_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV3335080.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPVGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21216..22259
                     /locus_tag="VYQ93_000330"
     CDS             21216..22259
                     /locus_tag="VYQ93_000330"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV3335081.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22343..23716
                     /locus_tag="VYQ93_000331"
     CDS             22343..23716
                     /locus_tag="VYQ93_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV3335082.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIERAFENGAAATLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTTYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGVELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLSANDQILLKGSNSMNLAMLVE
                     SLENETK"
     gene            23739..24314
                     /locus_tag="VYQ93_000332"
     CDS             23739..24314
                     /locus_tag="VYQ93_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3335083.1"
                     /translation="MIALTDTGLTFTKEPFDRERYEDLRSLLSEMLNQVSDLDADEVA
                     ELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEVGYSPTENILKEI
                     EEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQENQEIADLQFFAID
                     QLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24405..25109
                     /locus_tag="VYQ93_000333"
     CDS             24405..25109
                     /locus_tag="VYQ93_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV3335084.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25328..26701
                     /gene="ftsA"
                     /locus_tag="VYQ93_000334"
     CDS             25328..26701
                     /gene="ftsA"
                     /locus_tag="VYQ93_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV3335085.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26718..27977
                     /gene="ftsZ"
                     /locus_tag="VYQ93_000335"
     CDS             26718..27977
                     /gene="ftsZ"
                     /locus_tag="VYQ93_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV3335086.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27982..28653
                     /locus_tag="VYQ93_000336"
     CDS             27982..28653
                     /locus_tag="VYQ93_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV3335087.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28663..29202
                     /locus_tag="VYQ93_000337"
     CDS             28663..29202
                     /locus_tag="VYQ93_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV3335088.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29202..29465
                     /locus_tag="VYQ93_000338"
     CDS             29202..29465
                     /locus_tag="VYQ93_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV3335089.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29462..30247
                     /locus_tag="VYQ93_000339"
     CDS             29462..30247
                     /locus_tag="VYQ93_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV3335090.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30256..31044
                     /gene="divIVA"
                     /locus_tag="VYQ93_000340"
     CDS             30256..31044
                     /gene="divIVA"
                     /locus_tag="VYQ93_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV3335091.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31392..34184
                     /locus_tag="VYQ93_000341"
     CDS             31392..34184
                     /locus_tag="VYQ93_000341"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV3335092.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10558 a         6081 c         7499 g        10250 t
ORIGIN      
        1 ggtaacaaaa ctaatttaga gaatgaaata tagagtattt ctctcttaaa agtttttggt
       61 gaaacgagat gtagaaagga gatttagcca aagagtctat tagtgctaga ataatagatt
      121 agaattattt tagaaaaacg aagtgagcag cttataaatt caagtcccca aatagattca
      181 tactagtatc ttttgcaaaa aataaagggc gacttccttc atgaatatca atttcatcta
      241 taaggaaggt agctaattga actaacttat ttattctgtt tgtcgctaga aaaatcagac
      301 ctccttgtga agattgagga gatactgaat gaaaatcaaa gaagaaacta gcaagctagt
      361 agcagattgc ccaaaacacc gctttgaggt tgtagataag actgacctat ataatccaag
      421 gtgaagcgac tgtggtttga agagattttc aaagagtata ggctagagag tagtgttttt
      481 atgtccttct agtagaaaat gctagacaga agaatgggga acttggatag gaaaaataga
      541 ttgagaaagg aggttagaag agatgattat tacaaaaatt agccgtttag gaacttatgt
      601 gggagtcaat ccacattttg caacattaat agattttcta gaaaaaacag gactagaaaa
      661 tttaacagaa ggttcgattg ctatcgatgg taatcgattg tttgggaatt gctttattta
      721 tctagcagat ggtcaagcag gggctttctt tgaaacacac caaaaatatt tggatattca
      781 tttagttttg gaaaacgaag aagccatggc tgttacatcg ccggaaaatg taagcgttac
      841 ccaagaatat gatgaagaga aagatattga attatacaca gggaaagtgg aacagttggt
      901 tcatttgaga gctggtgaat gcctcatcac ttttccagaa gatttacatc aacccaaggt
      961 tcgtataaat gatgaacctg tgaaaaaagt tgtctttaaa gttgcgattt cttaatgtag
     1021 aaagagaaga acgatgaaaa aaatgagaaa gtttttatgt ctagctggaa ttgcgctagc
     1081 ggctgttgcc ttggtagctt gttcaggaaa aaaagaagct acaactagta ctgaaccacc
     1141 aacagaatta tctggtgaga ttacaatgtg gcactccttt actcaaggac cccgtttaga
     1201 aagtattcaa aaatcagcag atgctttcat gcaaaagcat ccaaaaacga aaatcaagat
     1261 tgaaacattt tcttggaatg acttctatac taaatggact acaggtttag caaatggaaa
     1321 tgtgccagat atcagtacag ctcttcctaa ccaagtaatg gaaatggtca actcagatgc
     1381 tttggttccg ctaaatgatt ctatcaagcg tattggacaa gataaattta acgaaactgc
     1441 cttaaatgaa gcaaaaatcg gagatgatta ctactctgtt cctctttatt cacatgcaca
     1501 agtcatgtgg gttagaacag atttgttaaa agaacataat attgaggttc ctaaaacttg
     1561 ggatcaactc tatgaagctt ctaaaaaatt gaaagaagct ggagtttatg gcttgtctgt
     1621 tccgtttgga acaaatgact taatggcaac acgtttcttg aacttctacg tacgtagtgg
     1681 tggaggaagc ctcttaacaa aagatcttaa agcagacttg acaagccaac ttgctcaaga
     1741 tggtattaaa tactgggtta aattgtataa agaaatctca cctcaagatt ctttgaactt
     1801 taatgtcctt caacaagcta ccttgttcta tcaaggaaaa acagcatttg actttaactc
     1861 tggcttccat atcggaggaa ttaatgccaa tagtcctcaa ttgattgatt cgattgatgc
     1921 ttatcctatt ccaaaaatca aagagtctga taaagaccaa ggaattgaaa cctcaaacat
     1981 tccaatggtt gtttggaaaa attcaaaaca tccagaagtt gctaaagcat tcttagaagc
     2041 actttataat gaagaagact acgttaaatt ccttgattca actccagtag gtatgttgcc
     2101 aactattaag gggattagcg attctgcagc ctataaagaa aatgaaactc gtaagaaatt
     2161 taaacatgct gaagaagtaa ttactgaagc tgttaaaaaa ggtactgcta ttggttatga
     2221 aaatggacca agtgtacaag ctagtatgtt aactaaccaa cacattattg aacaaatgtt
     2281 ccaagatatc attacaaatg gaacagatcc tatgaaagca gcaaaagaag cagaaaaaca
     2341 attaaacgat ttatttgagg ctgttcagta gatgtaaaag actagaaaat aggtgggcta
     2401 gtgagctgaa aagctctagc ccaatcttgt aaaagaaggg agaaggagaa tggttaaaga
     2461 acgtaattta actcgctgga tatttgtttt gccagctatg attatcgtag gattactctt
     2521 tgtttatccg tttttctcga gtatttttta tagctttacc aataagcatt tgattatgcc
     2581 taattataaa tttgttggtt tggctaacta taaagctgtg ctatcagatc ccaacttctt
     2641 taatgcgttc tttaattcaa ttaagtggac cattttctca ttagttggtc aagttttagt
     2701 agggtttgta ttggctttag ctcttcacag agtacgccac ttcaagaaat tatataggac
     2761 cttattgatt gttccttggg catttcctac catcgtcatt gccttttctt ggcagtggat
     2821 tctaaacggg gtttatggct acttacctaa tctaatcgta aaattaggtt taatggaaca
     2881 tacacctgca tttttgacag atagtacatg ggcattccta tgtttggtgt ttatcaacat
     2941 ttggtttgga gcaccaatga ttatggttaa tgtgctttca gctttgcaaa cagtaccaga
     3001 agaacaattt gaggctgcta agatagatgg tgcttcaagt tggcaggtgt tcaagtttat
     3061 cgtctttcca catattaaag tggttgtagg acttctagtt gttttgagaa ctgtatggat
     3121 ctttaataac tttgacatta tctacctcat tactggtggt ggaccagcca atgctacaac
     3181 gacgcttcca atttttgctt acaacctggg ctggggaact aaattgttgg gtcgtgcttc
     3241 agcagttaca gtattgctct ttatcttctt ggtggcgatt tgctttatct actttgctat
     3301 catcagtaag tgggaaaagg agggtagaaa ataatgaaga agaaatccag tatttattta
     3361 gatattctct cacatgtact tttagttggt gcgaccatcg ttgcaatttt cccattggta
     3421 tggattatca tatcttctgt caaagggaaa ggggaattaa ctcagtatcc aacacgattt
     3481 tggcctgaac agtttacatt agattatttc actcatgtta tcaacgattt gcacttcatt
     3541 gataacattc gaaacagttt aatcattgcc ttggctacaa ccctcattgc gattattatt
     3601 tctgctatgg cagcctatgg tattgttcga ttctttccta aattgggagc aatcatgtcg
     3661 agactactcg tcattaccta cattttccca ccaattttgt tagcaattcc ctattcaatt
     3721 gccattgcta aagttgggtt aacaaatagt ttatttggct tgatgatggt ttatctatct
     3781 tttagtgttc catatgcagt ttggctctta gttggatttt tccaaacagt tccaattgga
     3841 attgaagaag cggctagaat tgatggtgca aataaatttg ttacgtttta taaagttgtg
     3901 ctaccgattg tagcaccagg tattgtagca acagctattt atacatttat caatgcttgg
     3961 aatgaattcc tgtatgcctt gattttgatt aacaatacag gaaagatgac agtagcagta
     4021 gcccttcgtt cacttaatgg ttcagaaata ctagactggg gagatatgat ggcagcgtct
     4081 gttattgtag ttcttccatc aattattttc ttctctatca tccaaaataa gattgcaagt
     4141 ggattatcag aaggatctgt gaagtagacg aaagaaggaa aaaaatgaat aaaagaggtc
     4201 tttattcaaa actaggaatt tctgttgtag gcattagtct tttaatggga gtccccgctt
     4261 tgattcatgc gaatgaatta aactatggtc aactgtccat atctcctatt tttcaaggag
     4321 gttcatatca actgaacaat aagagtatag atatcagccc tttgttatta gataaattgt
     4381 ctggagatag tcagacagta gtaatgaaat ttaaagcaga taaaccaaac tctcttcaag
     4441 ctttgtttgg cctatctaat agtaaagcag gctttaaaaa taattacttt tcaattttca
     4501 tgagagattc tggtgagata ggtgtagaaa taagagacgc ccaaaaggga ataaattatt
     4561 tattttctag accagcttca ttatggggaa agcataaagg acaggcagtt gaaaatacac
     4621 tagtatttgt atctgattct aaagataaaa catacacaat gtatgttaat ggaatagaag
     4681 tgttctctga aacagttgat acatttttgc caatttcaaa tataaatggt atagataagg
     4741 caacactagg agctgttaat cgtgaaggta aggaacatta cctcgcaaaa ggaagtattg
     4801 atgaaatcag tctatttaac aaagcaatta gtgatcagga agtttcaaat attcccttgt
     4861 caaatccatt tcagttaatt ttccaatcag gagattctac tcaagctaac tattttagaa
     4921 taccgacact atatacatta agtagtggaa gagttctatc aagtattgat gcacgttatg
     4981 gtgggactca tgattctaaa agtaagatta atattgccac ttcttatagt gatgataatg
     5041 ggaaaacgtg gagtgagcca atttttgcta tgaagtttaa tgactatgag gagcagttag
     5101 tttactggcc acgagataat aaattaaaga atagtcaaat tagtggaagt gcttcattca
     5161 tagattcatc cattgttgaa gataaaaaat ctgggaaaac gatattacta gctgatgtta
     5221 tgcctgcggg tattggaaat aataatgcaa ataaagccga ctcaggtttt aaagaaataa
     5281 atggtcatta ttatttaaaa ctaaagaaga atggagataa cgatttccgt tatacagtta
     5341 gagaaaatgg tgtcgtttat gatgaaacaa ctaataaacc tacaaattat actataaatg
     5401 ataagtatga agttttggag ggaggaaagt ctttaacagt cgaacaatat tcggttgatt
     5461 ttgatagtgg ctctttaaga gaaaggcata atggaaaaca ggttcctatg aatgttttct
     5521 acaaagattc gttatttaaa gtgactccta ctaattatat agcaatgaca actagtcaga
     5581 atagagtaga gagttgggaa caatttaagt tgttgcctcc gttcttagga gaaaaacata
     5641 atggaactta cttgtgtcct ggacaaggtt tagcattaaa atcaagtaac agattgattt
     5701 ttgcaacata tactagtgga gaactaacct atctcatttc tgatgatagt ggtcaaacat
     5761 ggaagaaatc ctcagcttca attccgttta aaaatgcaac agcagaagca caaatggttg
     5821 aactgagaga tggtgtgatt agaacattct ttagaaccac tacaggtaag atagcttata
     5881 tgactagtag agattctgga gaaacatggt cggaagtttc gtatattgat ggaattcaac
     5941 aaacttcata tggcacacaa gtatctgcaa ttaaatactc tcaattaatt gatggaaaag
     6001 aagcagtcat tttgagtaca ccaaattcta gaagtggccg taagggaggc caattagttg
     6061 tcggtttggt caataaagaa gatgatagta ttgattggag ataccactat gatattgatt
     6121 tgccttcgta tggttatgcc tattctgcga ttacagaatt gccaaatcat cacataggtg
     6181 tactgtttga aaaatatgat tcgtggtcga gaaatgaatt gcatttaagc aatgtagttc
     6241 agtatataga tttggaaatt aatgatttaa caaaataaag gagaaaaaca tggttaaata
     6301 cggtgttgtt ggagcagggt attttggagc tgaattggct cgctatatgc aaaagaatga
     6361 tggagcagag attactcttc tctatgatcc agataatgca gaggcgattg cagaagaatt
     6421 gggagcaaaa gtagcaagtt ccttagatga gttggtttct agcgatgaag tagattgtgt
     6481 tatcgtcgca actccaaata atcttcataa ggaaccggtt attaaggctg cacagcatgg
     6541 taaaaatgtt ttctgtgaaa aaccaattgc gctttcttat caagattgtc gcgagatggt
     6601 agatgcgtgt aaagaaaaca atgtaacctt tatggcagga catattatga atttctttaa
     6661 tggtgttcat catgcaaaag aactcattaa tcaaggagtt atcggagacg ttctatattg
     6721 tcatacagct cgtaatggtt gggaagaaca acaaccgtca gtatcatgga aaaaaattcg
     6781 tgaaaaatca ggtggtcact tgtatcacca catccatgaa ttggattgcg ttcaattcct
     6841 tatggggggc atgcctgaaa ctgtaaccat gacaggtgga aatgtggccc atgaaggtga
     6901 acatttcggt gatgaagatg atatgatttt tgtcaatatg gaattttcta ataagcgttt
     6961 tgctttgtta gaatggggtt cagcttatcg ttggggtgaa cattatgtct taatccaagg
     7021 aagcaaaggt gccatccgct tagacttatt caactgtaaa ggaactctta agctagatgg
     7081 gcaagaaagc tatttcttga ttcacgaatc gcaagaagaa gatgatgatc ggactcgtat
     7141 ctatcatagt acagagatgg atggagcaat tgcttatggt aaaccaggta aacgtactcc
     7201 attatggcta tcatctgtca ttgataaaga aatgcgctat ctgcatgaga ttatggaagg
     7261 agctccagta tcagaagaat ttgcaaaact tttgacaggt gaagctgccc tagaagcaat
     7321 tgctactgca gatgcttgta cccagtctat gtttgaagat cgcaaagtaa gattgtcaga
     7381 aattgtaaaa taaattttgg tattctccta tctataggtc gacttgctcc tctgaaagta
     7441 cttttagagg agctgtttga ctttgctagt ttttgaaact gaaatctatt atactacaaa
     7501 ctattgaaag cgttttaatt ttaaggtata ataatctcat agaaataaag aaaaggagga
     7561 aagaggatgc cacagattag caaagaagcc ttgattgagc aaatcaaaga tggaatcatc
     7621 gtttcttgtc aggctcttcc tcatgaaccg ctttatacag aagcgggagg ggtgattccc
     7681 ttgctggtca aagcggctga gcaaggtgga gcagtcggta tccgagcaaa cagtgttcgc
     7741 gatatcaagg aaattaagga agtcactaaa cttccaatca ttgggattat caaacgtgat
     7801 tatccacctc aggaaccctt catcacggct actatgaaag aagttgatga attggcagaa
     7861 ctggacatcg aggtgattgc tctggattgt accaagcgtg aacgctacga tggtttggaa
     7921 attcaagagt tcattcgtca ggttaaggag aaatatccta atcagctttt gatggctgat
     7981 actagtatct tcgaagaagg gctagcagct gtagaagcag gaattgactt tgtcggaaca
     8041 accttatcag gctacacatc ctacagtcca aaagtagacg gtccagattt tgaattgatt
     8101 aagaaactct gtgatgccgg tgtagatgtc attgcagaag gaaaaattca tacaccagaa
     8161 caagccaaac aaatccttga atatggagtg cgaggcatcg ttgttggtgg cgccattact
     8221 agaccaaaag agattacaga acgcttcgtt gctagtctta aataagatgt gagagggagt
     8281 tttatgttta aagttttaca aaaagttgga aaagctttta tgttacctat agctatactt
     8341 cctgcagcag gtctactttt ggggattggt ggtgcacttt caaacccaac cacgatagca
     8401 acttatccaa tactagacaa tagtattttt caatcaatat tccaagtaat gagctctgca
     8461 ggagaggttg tattcagtaa tttgtcacta cttctctgtg tgggattatg tattggctta
     8521 gcgaaacgag ataaaggaac cgctgcgtta gcaggagtaa ctggttactt agttatgact
     8581 gcaacgatca aagctttggt aaaacttttt atggcagaag gatctgcaat tgatactgga
     8641 gttattggag cattagttgt cggaatagtt gccgtatatt tgcacaaccg atataacaat
     8701 attcaattac cttccgcttt aggattcttt ggaggttcac gcttcgttcc tattgttaca
     8761 tcgttctctt ctatcttgat tggctttgtc ttctttgtta tttggccacc tttccaacaa
     8821 cttcttgttt ctacaggtgg atatatttct caggcgggtc caattggaac ttttctatat
     8881 ggatttttaa tgagactttc tggagcagta ggcttacatc atataattta ccctatgttt
     8941 tggtatactg aacttggtgg tgttgaaact attgcaggac aaacagtggt tggagctcaa
     9001 aaaatatttt ttgctcaatt agccgatccg gcccattctg gattatttac agaaggaaca
     9061 aggttttttg caggtcgttt ctcaacaatg atgttcggtt taccggctgc ctgtttagcg
     9121 atgtaccata gtgttcctaa aaatcgtcgt aaaaaatacg cgggtttgtt ttttggagtt
     9181 gctttaacat cttttattac cggtattaca gaaccaattg aatttatgtt tctattcgtc
     9241 agtccggttc tatatgttgt tcacgcattc cttgatggtg ttagcttctt tattgcagac
     9301 gtcttaaata tttcaatagg aaacacattt tcaggaggtg taatcgattt cactttattt
     9361 ggaattttgc aggggaacgc taagacgaat tgggttcttc agattccatt tggacttatt
     9421 tggagtgttt tgtattatat tatttttaga tggttcatta ctcaattcaa cgttctaacg
     9481 ccagggcgag gagaagaagt agattctaaa gaaatttctg aatccgcaga ttcaacttca
     9541 aatactgcag attatttaaa acaggatagc ctacaaatta tcagagcctt gggtggatca
     9601 aataatatag aagatgtaga tgcttgtgtg acacgtttac gtgtagctgt aaaagaagtt
     9661 aatcaagttg ataaagcact tttaaaacaa attggtgcag ttgatgtctt agaagtgaag
     9721 ggtggcattc aagcaatcta tggagcaaaa gcaatcttat ataaaaatag tattaatgaa
     9781 attttaggtg tagatgatta agtacttact gacttaataa aaaacagagg agagtgatgg
     9841 atgagtagga tgaaatgaaa tcgcatacaa gaaataaaga actcattatc caagttggat
     9901 acgcttatta cataggagaa tacaaatgaa atttagaaaa ttagcttgta cagtacttgc
     9961 gggtgctgcg gttcttggtc ttgctgcttg tggcaattct ggcggaagta aagatgctgc
    10021 caaatcaggt ggtgacggtg ccaaaacaga aatcacttgg tgggcattcc cagtatttac
    10081 ccaagaaaaa actggtgacg gtgttggaac ttatgaaaaa tcaatcatcg aagcgtttga
    10141 aaaagcaaac ccagatataa aagtgaaatt ggaaaccatc gacttcaagt caggtcctga
    10201 aaaaatcaca acagccatcg aagcaggaac agctccagac gtactctttg atgcaccagg
    10261 acgtatcatc caatacggta aaaacggtaa attggctgag ttgaatgacc tcttcacaga
    10321 tgaatttgtt aaagatgtca acaatgaaaa catcgtacaa gcaagtaaag ctggagacaa
    10381 ggcttatatg tatccgatta gttctgcccc attctacatg gcaatgaaca agaaaatgtt
    10441 agaagatgct ggagtagcaa accttgtaaa agaaggttgg acaactgatg attttgaaaa
    10501 agtattgaaa gcacttaaag acaagggtta cacaccaggt tcattgttca gttctggtca
    10561 agggggagac caaggaacac gtgcctttat ctctaacctt tatagcggtt ctgtaacaga
    10621 tgaaaaagtt agcaaatata caactgatga tcctaaattc gtcaaaggtc ttgaaaaagc
    10681 aactagctgg attaaagaca atttgatcaa taatggttca caatttgacg gtggggcaga
    10741 tatccaaaac tttgccaacg gtcaaacatc ttacacaatc ctttgggcac cagctcaaaa
    10801 tggtatccaa gctaaacttt tagaagcaag taaggtagaa gtggtagaag taccattccc
    10861 atcagacgaa ggtaagccag ctcttgagta ccttgtaaac gggtttgcag tattcaacaa
    10921 taaagacgac aagaaagtcg ctgcatctaa gaaattcatc cagtttatcg cagatgacaa
    10981 ggagtgggga cctaaagacg tagttcgtac aggtgctttc ccagtccgta cttcatttgg
    11041 aaaactttat gaagacaaac gcatggaaac aatcagcggc tggactcaat actactcacc
    11101 atactacaac actattgatg gatttgctga aatgagaaca ctttggttcc caatgttgca
    11161 atctgtatca aatggtgacg aaaaaccagc agatgctttg aaagccttca ctgaaaaagc
    11221 gaacgaaaca atcaaaaaag ctatgaaaca atagtcctta gttattctat aaaaagtagt
    11281 tttttaaaga acctaagagt gtataccccc ttttccctct acacagatag tgtaagaaaa
    11341 gggggctttt gtttaaaatg taagaaactg tcacgaaatt aaaatgaagt tcttacataa
    11401 gcgaatcata aaaaatttca ttttgatttt aaaacagttc aagaaagtca aaaaattatt
    11461 ctatttgaaa gagaggtgcc gactgtgaaa gtcaataaaa tccgtatgcg ggaaacagtg
    11521 atttcctacg ctttcctagc accagtatta ttcttctttg tcatctttgt gttggctccg
    11581 atggtgatgg gcttcattac aagtttcttt aactactcaa tgactaaatt tgagtttgta
    11641 ggcttggata actatatccg tatgtttaaa gatcctgtct ttacaaaatc tctgattaac
    11701 acagttattt tggttattgg atctgtacca gttgttgttc tattctcact ctttgtagca
    11761 tctcagacct atcatcaaaa tgtcattgcc agatccttct accgtttcgt cttcttcctt
    11821 cctgttgtaa cgggtagtgt tgccgtgaca gttgtttgga aatggattta tgacccacta
    11881 tcagggattc taaactttgt ccttaagtcc agccacatca tcagccaaaa catttcttgg
    11941 ttgggagata aaaactgggc attgatggcg attatgatta ttctcttgac cacttcagtt
    12001 ggtcagccca tcatccttta tatcgctgcc atggggaata ttgacaattc actggttgaa
    12061 gcggcgcgtg ttgatggtgc aactgagttt caagtttttt ggaagattaa atggccaagc
    12121 cttcttccaa caactcttta tattgcaatc atcacaacaa ttaactcatt ccagtgtttc
    12181 gccttgattc agcttttgac atctggtggt ccaaactact caacaagtac cttgatgtac
    12241 tacctttacg aaaaagcctt ccaattgaca gaatacggct atgccaacac aattggtgtc
    12301 ttcttggcag tcatgattgc tatcgtaagc tttgttcaat ttaaagtact tggaaacgac
    12361 gtagaatact aaagaaagga gacagctatg caatctacag aaaaaaaacc attaacagcc
    12421 tttactgtta tttcaacaat cattttgctc ttgttgactg tgctgttcat ctttccattc
    12481 tactggattt tgacaggggc attcaaatca caacctgata caattgttat tcctcctcag
    12541 tggttcccta aaatgccaac catggaaaac ttccaacaac tcatggtgca gaaccctgcc
    12601 ttgcaatgga tgtggaactc agtatttatc tcattggtaa ccatgttctt agtttgtgca
    12661 acctcatctc tagcaggtta tgtattggct aaaaaacgtt tctatggtca acgcattcta
    12721 tttgctatct ttatcgctgc tatggcgctt ccaaaacaag ttgtccttgt accattggta
    12781 cgtatcgtca acttcatggg aatccacgat actctctggg cagttatctt gcctttgatt
    12841 ggatggccat tcggtgtctt cctcatgaaa cagttcagtg aaaatatccc tacagagttg
    12901 cttgaatcag ctaaaatcga cggttgtggt gagattcgta ccttctggag tgtagccttc
    12961 ccgattgtga aaccagggtt tgcagccctt gcaatcttta ccttcatcaa tacttggaat
    13021 gactacttca tgcaattggt aatgttgact tcacgtaaca atttgaccat ctcacttggg
    13081 gttgcgacca tgcaggctga aatggcaacc aactatggtt tgattatggc aggagctgcc
    13141 cttgctgctg ttccaatcgt cacagtcttc ctagtcttcc aaaaatcctt cacacagggt
    13201 attactatgg gagcggtcaa aggataatac tctgcgaaaa tcgaatgcaa actacgtcag
    13261 cttcaccttg ccatacttaa gtattgcctg cggttagctt cctagtttgt tcttcaattt
    13321 tcattgagta taggaaaatc aatctatcaa gatacagaag tatattttat agatttagag
    13381 aatatagagg ttataagtgt ctacaaaatg gagggtatgc agttacttta tgaagttttg
    13441 tcagacgctt atcaacttaa gaatggtttt agttaactat cagaaacgaa ggaaagagta
    13501 tgatttttga cgatttgaaa aacatcacct tttacaaagg gattcatccc aatttagaca
    13561 aggctatcga ctacctctac caacatcgta aagattcatt cgaattagga aagtatgaga
    13621 ttgatggaga taaagtcttt ctagttgttc aggaaaatgt cctcaatcaa gttgagaata
    13681 atcaatttga acaccataag aactatgcag atttgcattt gctggtagaa ggacatgaat
    13741 attcgagcta cggttcacgt atcaaagacg aggcagtagc attcgacgaa gcgactgaca
    13801 ttggctttgt tcattgtcat gaacactacc cactcttgtt gggttatcat aattttgcga
    13861 ttttcttccc aggtgagcca catcagccaa ctggctatgc aggtatggaa gaaaaggttc
    13921 gaaaatatct ctttaaaatt ttgattgatt aaaaatagga tgaattgttt ttttgtaaag
    13981 ctttgacaat actctaccat gaaattgatc tttgtgaggt agagaaatga gaataaaata
    14041 tttaaaaatt ggtatcttct aagcatgctg caagagctag tttcttagat ggacagggga
    14101 ttacagttga tgagatgtcc tggataatta atggcattgt gaatgcattg attggtagat
    14161 acataaaatt aggtacttat gcagctaagt atggtattag tatagcacgc tcgatcttaa
    14221 gtagggcagc tgcaactgca gcagcaagag taggattact gaccaagatt tctggatgga
    14281 ttttacgagt agctgtgaat gtagctgatg tatatggtaa ttttgccaac aatattgctg
    14341 cagcttggga tgcatatgat aaaattccta acaatggtcg tataaacttt taaaatgcga
    14401 gaatgaaagc actttgtatt tttttattga atatgttagc ttggacagtg cttgcaatga
    14461 taattcgtgg agggctagat ggatttgata ggcatacttg gagtactatt ttaattgctt
    14521 cgctgttcgg ggtatatgat tataagccca tagataaaaa tagaaaaaag tccaaaagaa
    14581 aaaagaaaaa atagatttgt tcatggtagg gacttatgaa agctttactg acaaaaaaga
    14641 aaacagttta caaagaaaaa tgatggagga gcaaacatgg cacaaaaagg agtaagcctt
    14701 atcaaggcag catttgatac agataacttt ctcatgcgtt ttagtgagaa ggtcttggac
    14761 atcgtgacag ccaatcttct ttttgtcgtc tcttgtttgc ccatcgtgac gattggagtg
    14821 gctaaaatca gcctctacga gaccatgttc gaagttaaga agagcagacg ggtgcctgtt
    14881 tttaaaatct atctaagatc tttcaagcaa aatctgaaac taggtcttca gttaggtctg
    14941 cttgagttgg gcattgtttc attaagcctc ctagatcttt atctcttttg gggtcaaaca
    15001 gctctgccct ttcaattgct gaaagctatt tgtctaggca ttctgatttt ccttacgatt
    15061 gtgatgttgg ctagctatcc aatcgcggca cgttatgatt taacttggaa agaaattctt
    15121 caaaaaggat tgatgttggc tagttttaac tttccttggt tcttcctcat gttagccatt
    15181 cttgtcctca ttgtgatggt tctttatctg tctgccttca gtctactctt aggtggctca
    15241 gtcttcctgc tttttgggtt tggactattg gtctttatcc agactggatt gatggagaaa
    15301 attttcgcaa aataccaata ggagccttat ttctgaaact actttcaaag gctccaaacg
    15361 ctattctata agcgagaaac taaaatctaa gtgtctataa gatattgaaa gccttttcgg
    15421 taaagtgtat actaaagaag taaagaaaaa tctgctttag cagaaaataa aaataatagg
    15481 agaaaatcta tgtcagattt gaaaaaatac gaaggtgtca ttccagcctt ctacgcatgt
    15541 tatgatgagc aaggagaagt aagcccagaa cgtacgcgtg ccttggttca atacttcatt
    15601 gataaaggtg ttcaaggtct ttatgtcaat ggttcttctg gtgaatgtat ctaccaaagt
    15661 gttgaagacc gcaagttaat cttggaagaa gttatggcag ttgccaaagg taaattgacc
    15721 attattgccc atgttgcttg caataatact aaagatagta tggaacttgc tcgccatgct
    15781 gaaagcttgg gagtagatgc tattgcaacg attccaccaa tttatttccg cttgccagaa
    15841 tactcagttg ccaaatactg gaacgatatc agttctgcag ctccaaacac agactacgtg
    15901 atttacaaca ttcctcaatt ggcaggggtt gctttgactc caagccttta cacagaaatg
    15961 ttgaaaaatc ctcgtgttat cggtgtgaag aactcttcta tgccagttca agatatccaa
    16021 acctttgtca gccttggtgg agaagaccat atcgtcttta atggtcctga tgagcagttc
    16081 ctaggaggac gcctcatggg ggctagggct ggtatcggtg gtacttatgg tgctatgcca
    16141 gaactcttct tgaaactcaa tcagttgatt gcggataagg acctagacac agcgcgtgaa
    16201 ttgcagtatg ctatcaacgc aatcattggt aaactcactt ctgctcatgg aaatatgtac
    16261 ggtgtcatca aagaagtctt gaaaatcaat gaaggcttga atattggatc tgttcgttca
    16321 ccattgacac cagtgactga agaagatcgt ccagttgtag aagcggctgc tgccttgatt
    16381 cgtgaaacca aggagcgctt cctctaatct aaaaggaggt atttatgaca tattacgttg
    16441 caattgatat cggtggaacc aacatcaagt atggtttggt tgatcaagag gggcaacttc
    16501 ttgaatcgca tgaaatgcca actgaggcgc ataagggtgg acctcatatc ttacaaaaga
    16561 ccaaagatat cgtagctagt tatttagaaa aaggcccagt agcaggtgtt gccatatctt
    16621 ctgctgggat ggtggatccg gataagggtg agattttcta tgctgggccg caaatcccta
    16681 actacgcagg cacccagttc aaaaaggaaa tcgaagaaag ctttactatt ccttgtgaga
    16741 ttgaaaatga tgtcaactgt gcaggtcttg ctgaggcagt atctggttca ggcaagggag
    16801 caagtgtgac actttgcttg accattggaa caggtatcgg tggttgcttg attatggata
    16861 ggaaagtctt ccatggtttt agcaattcag cctgtgaagt cgggtatatg catatgcagg
    16921 atggagcttt tcaagacttg gcttctacaa cagctttagt gaaatatgta gctgaagccc
    16981 atggagaaga tgttgatcag tggaatggcc gtagaatttt caaagaagcc actgaaggaa
    17041 acaaaatctg catggaaggt attgaccgta tggttgacta tctaggaaaa ggtctggcaa
    17101 atatttgcta tgttgccaat ccagaagtgg ttattcttgg tggtggtatc atggggcaag
    17161 aggctatcct caaacctaag atccgcacag ccttgaaaga ggctttggta ccaagtttag
    17221 cagaaaaaac acgattagaa tttgcccatc accaaaatac agcagggatg ttgggtgcat
    17281 attatcattt taagacaaaa caatcctagt ttggctcagc caaactagga ttttcttaca
    17341 cgtttttgtc tacgatagcc gttgagtttt ttattttccc agtagctatt aaagattttt
    17401 tccttgcttt cgcgattgat ttccaaaaag taggcataaa tcaaatcgat aaagaagagc
    17461 ataggaagtt gagcggatat tcgttggata taggagggtt ggctgtgggt ggctacaaga
    17521 acagtctctg tataggtctg gctatcttta ttgggaacac ttgtaaagag tacagtcttt
    17581 gcccccatct ccttggcatc taatagacta tctaaaatag aaggagttga gcctgaaagt
    17641 gagaagccca gtactagaca attttcatcc atgatgctgg ttgtccaggc aaagccgtct
    17701 tggtctgtca aagcttcgca gaccacacct agtcgcataa aacgtaattt catttcacgg
    17761 gcgacgaggc cagaactccc tgttccaaag aagtagatac gctcagcatc ttcgattagc
    17821 tgggcaattc gttctagttg gatttcgtca atcaagtctt gtgtttgttc cctcatattg
    17881 ctataacttc tgaggactcg tttggtcagt ggactgtgct tggagacttg gttggcttga
    17941 ttttctgcct gatgttggta ttggaaaata aattctcggt agccagtaaa gccacacttt
    18001 ttagcaaagc gggtcaaagc agcttgagaa atatgtaatt tttgggtgac ttgttgagaa
    18061 gataaatcat ctgtaatcgt ttcagcttgc aaaaaatagc gagcgatttc ttgttctagg
    18121 tctgtcattt cttcaaaatg tgaatcaatg atagttgcga tatctggttt gtccataggg
    18181 aaagctcctt tacatgagtc atactggaag actagatcag ggaatagtca cacttcatta
    18241 taacacataa tataaggata gataaataaa aacgcatctc tgttttaaaa acgaaaaaat
    18301 cgaaaaagct tcttactttt ccataatttt ctactcaaat tgtggtacaa ttaagagtaa
    18361 gattttaagt tagaaatgag actgatttgt atgagaaaat ttaacagcca ttcgattccg
    18421 attcggctta atttattgtt ttcaatcgtc attttactct ttatgaccat tattggtcgt
    18481 ttgttgtata tgcaggtttt gaacaaggat ttttacgaaa aaaagctagc ctcagctagt
    18541 cagaccaagg tcacaaccag ttctgctcgt ggggaaattt atgatgctag tggaaaacct
    18601 ttggtagaaa atacgttaaa acaggttgtt tcctttacgc gtagcaataa aatgacggct
    18661 acagacttaa aagaaacagc taaaaagtta ctgacttatg tgagcatcag ttcgccaaat
    18721 ttgacagaac gccagctggc tgattactat ttggctgatc ctgaaatcta taaaaaaaca
    18781 gtggaagctc tcccaagtga gaaacgcttg gattcagatg gtaatcgcct atccgaatca
    18841 gaactgtata acaatgcggt cgatagtgtc ccaacaagtc aactaaacta tacagaggat
    18901 gaaaagaaag aaatctatct ttttagtcag ttaaatgctg ttggaaactt tgcgacagga
    18961 accattgcga cagatcctct aaatgattct caggtggctg ttattgcctc tatttcaaag
    19021 gagatgcctg gcattagtat ttctacttct tgggatcgaa aggttttgga aacttccctt
    19081 tcttctatag tagggagtgt atccagtgaa aaagctggtc tcccagcgga agaagcagaa
    19141 gcctatctta aaaaaggcta ttctctaaat gaccgtgttg gaacctccta tttggaaaag
    19201 caatatgaag agaccttaca agggaaacgc tcggtaaaag aaatccatct ggataaatat
    19261 ggcaatatgg aaagtgtgga tacaattgag gaaggtagta agggaaacaa tatcaaactg
    19321 accattgatt tggccttcca agatagcgtg gatgctttgc tgaaaagtta tttcaattca
    19381 gagttgggaa atggtggagc caagtattct gaaggtgtct atgcagtcgc ccttaaccca
    19441 aaaacaggtg ctgttttgtc tatgtcaggg attaaacatg acttgaaaac gggagagttg
    19501 acgccggatt ccttgggaac ggtaaccaat gtctttgttc caggttcggt tgtcaaggcg
    19561 gcgaccatca gctcaggctg ggaaaatgga gtcttgtcag gaaatcagac cttgacagac
    19621 cagtccattg tcttccaagg ttcagctcca attaattctt ggtatactgc cttttctagg
    19681 ccaatgccga ttacggcagt tcaggctcta gagtattcat ccaatgctta tatggtccaa
    19741 acagccttag gtcttatggg gcaaacctat caaccaaata tgtttgtcgg caccagcaat
    19801 ctagagtctg ctatgggtaa attgcgttca acctttggcg aatatggctt gggttctgcg
    19861 acaggaattg acctaccaga tgaatctact ggatttattc ccaaagagta tagctttgct
    19921 aattacatta ctaatgcctt tgggcagttt gataactata ccccaatgca attggctcag
    19981 tatgtggcaa ctattgcaaa tgatggtgtt cgtgtggctc ctcgtattgt tgaaggcatt
    20041 tatggtaata atgataaggg aggactgggc gacttgattc agcaactgca accgacagag
    20101 atgaataagg tcaatatatc cgactccgat atgagtgtct tgcaccaagg tttttatcag
    20161 gttgctcatg ggactagcgg attgacaact ggacgtgcct tttcaaatgg tgccttgata
    20221 tccattagcg gaaaaacggg tacagccgaa agctatgtgg cagatggtca gcaagcaacc
    20281 aataccaatg cggtggccta tgccccatct gataatcccc aaatcgctgt tgcagtggtc
    20341 tttcctcata ataccaatct aacaaatggt gtagggcctt ccattgcgcg tgacattatc
    20401 aatctgtatc aaaaatacca tccgatgaac tagaaaggaa attatgcttt atccaacacc
    20461 tattgccaag ttgattgaca gttattctaa gttaccaggt atcgggatta agacggctac
    20521 gcgtctggcc ttttatacga ttgggatgtc tgctgatgat gtcaatgaat ttgcaaaaaa
    20581 tctcctttct gctaagagag aattgacata ttgttctatt tgtggacgtt tgacagacga
    20641 cgatccttgt tctatctgta ctgatccgac tcgtgaccag acaacaattt tagttcttga
    20701 ggatagtaga gatgtggcag ccatggaaaa tatccaagaa taccatggac tctatcatgt
    20761 cctgcatggc ctcatttctc ctatgaatgg tatcagtccg gacgatatca atctcaagag
    20821 ccttatgact cgtcttatgg atagtgaggt ttcagaagtg attgtggcga ctaatgctac
    20881 agcggatggt gaagcgactt ccatgtatct ttcacgtttg ctcaagccgg ttggtatcaa
    20941 ggttacgcgt ctagcacgag gtctcgctgt gggagcggac attgagtatg cggacgaagt
    21001 gacactctta cgagcgattg aaaatcggac agagttgtaa gtgtaggcaa atttacgaac
    21061 tcccttcatt tataaaaaat caaagaggct gaaaatcgtt cctatcggcc tctttttgta
    21121 tagtgtgatg agtaggatcg ggttcaagtt ttaaaaaacc aagcaaatat gatatactaa
    21181 agagcgagta ttctagtaga attaggacaa ataatatgaa acaaacgatt attcttttat
    21241 atggtggacg gagtgcggaa cgcgaagtct ctgtcctttc agctgagagt gtcatgcgtg
    21301 cggtcaatta cgaccgtttc acagtcaaga ctttctttat cagtcagtca ggtgacttta
    21361 tcaaaacaca ggaatttagt catgctccgg ggcaagaaga ccgtctcatg accaatgaaa
    21421 ccattgattg ggataagaaa gttgcaccaa gtgctatcta cgaagaaggt gcagtggtct
    21481 ttccagtcct tcacgggcca atgggagaag atggctctgt tcaaggattc ttggaagttt
    21541 tgaaaatgcc ttacgttggt tgcaacattt tgtcatcaag tcttgccatg gataaaatca
    21601 cgactaagcg tgttctggaa tctgctggta ttgcccaagt tccttatgtg gctatcgttg
    21661 aaggcgatga tgtgactgct aaaatcgctg aagtggaaga aaaattggct tatccagtct
    21721 tcactaagcc gtcaaacatg gggtctagtg tcggtatttc taagtctgaa aaccaagaag
    21781 aactccgtca agccttaaaa cttgccttcc gatatgacag ccgtgtcttg gttgagcaag
    21841 gagtgaatgc ccgtgaaatt gaggttggcc tcttgggtaa ctacgatgtc aagagcacgc
    21901 tacctggaga agttgtcaag gacgttgcct tttatgacta cgatgccaag tatattgata
    21961 acaagattac tatggatatt cctgccaaaa tcagtgatga tgtggtggct gtcatgcgtc
    22021 aaaatgcaga aacagccttc cgtgccattg gtggccttgg tctatctcgt tgcgatttct
    22081 tctatacaga taagggagag atttttctca acgagctcaa taccatgcca ggtttcaccc
    22141 agtggtctat gtacccacta ctttgggaca atatggggat cagctaccca gaactaatcg
    22201 agcgtttggt tgaccttgcc aaggaaagtt ttgacaagcg cgaagcgcat ttgatataaa
    22261 atgaaagaga gggtagaagc cagaaccatc actgcaaggt gactagagtt ctcggacttc
    22321 agcccttttt aaaggagtag aaatgaaatt aacaatccat gaaattgccc aagttgttgg
    22381 agccaaaaat gatatcagta tctttgagga cacccagtta gaaaaagctg agtttgatag
    22441 tcgtttgatt ggaactggag atttatttgt gccacttaaa ggtgcgcgtg acggtcatga
    22501 ctttatcgaa agagcttttg aaaatggtgc ggcagcaacc ctgtctgaga aagaggttgc
    22561 aaatcatccc tacattctag tagatgatgt tttgactgcc tttcaacaac tagctgccta
    22621 ctatcttgaa aaaacagctg ttgatgtctt tgcggttacg ggttcaaatg gcaagacaac
    22681 gactaaggat atgttggcgc atttactgtc aacgacctac aagacctaca aaacacaagg
    22741 caactacaat aacgagattg gccttcccta cacagttcta cacatgcctg aaggaacaga
    22801 aaagttggtt ttggagatgg ggcaggacca cttgggcgat attcatctct tgtctgaatt
    22861 ggctcgtcca aaaacagcca tcgtgacctt ggttggagaa gctcatttag cctttttcaa
    22921 agatcgttct gagattgcta aaggaaaaat gcaaattgca gacggaatgg cttcaggttc
    22981 cttgctttta gcgccggctg accctatcgt agaggactat ttgccaactg ataaaaaggt
    23041 ggttcgtttt gggcaagggg tagagctgga aattactgac ttggttgagc gcaaagatag
    23101 tctgaccttc aaggccaatt tcttagagca agtccttgat ttgccagtaa ctggcaagta
    23161 caatgcgaca aatgctatga ttgcatccta tgttgccttg caagaaggag tttcagagga
    23221 gcaaattcgt ttggccttcc aagatcttga attgacgcgt aaccgtaccg agtggaagaa
    23281 agcagccaat ggagcagata tcctgtcaga tgtttacaat gccaatccaa ctgctatgaa
    23341 actgatttta gagactttct ctgccattcc agccaatgaa ggtggcaaga aaattgcagt
    23401 tttggcggat atgaaggagc ttggtgacca gtctgttcaa cttcataatc agatgatttt
    23461 gagcctctcc ccagatgtgc ttgatatcgt tattttctat ggagaagaca ttgctgaatt
    23521 agcccaattg gccagtcaaa tgttcccaat tggccacgtt tactacttca agaaaacaga
    23581 agaccaagac caatttgaag acttagtcaa gcaggtcaag gaaagcctca gtgccaatga
    23641 tcaaatcttg ctcaagggct ctaactctat gaacctagcc atgttggtag aaagtttaga
    23701 aaatgaaacc aagtgatttt gttaagtatc tgtaaagaat gattgccctt acagatactg
    23761 gcttaacctt tacaaaagag ccttttgacc gtgagcgcta cgaagacttg cgaagtctgt
    23821 tatctgaaat gttgaatcag gtatcagacc ttgatgcaga cgaagtggca gaactcttga
    23881 aaccgacctc cgcttatgcg actcccttaa tggacgtccg tgcttggatt gttgaggatg
    23941 agaagatttg tctggttagg ggacaaggag aggatagttg ggctttgcca ggtggctttg
    24001 gtgaagtcgg ctattctcca actgaaaata ttctcaagga aattgaagaa gaaaccggtt
    24061 ttaaagccaa agttgaaaga ctgctagctg tttttgatac caatcgtttc caactacaga
    24121 gcaaacaata tgcaaagttt gtttttgaat gtaagcttct cgatggacaa ttccaagaaa
    24181 atcaagaaat cgctgacctt caattttttg caattgacca actgccgaat ttatctgaaa
    24241 aacgcattac caaggagcaa atcgagattc tttggcaggt ttatcaaggt cagagggagc
    24301 aatatcttga ctaagaagat gattatcgta tttctaaatt catttttctt aagtagcatg
    24361 gtataataaa atgcaggaaa attttgaatc atgaggaaga ctagatgaat ttatgggata
    24421 ttttctttac gactcaggca accgagccgc ccaaatttga ccttttttgg tatgttagcc
    24481 tatttacgct cttagcctta accttttata cagcccatcg ctatcgtgaa aagaaggttt
    24541 accaacgatt tttccaaatc ttgcagactg ttcagttaat ccttctttat ggttggtact
    24601 gggtcaatca tatgccactg tcagaaagcc taccctttta ccattgccgt atggctatgt
    24661 ttgtggtact cttgcttcct ggtcagtcca aatataaaca atactttgca ttattgggaa
    24721 catttgggac attagcagcc tttgtttatc cagtgccaga tgcttaccct tttccacata
    24781 tcaccattct atcctttatc tttggtcatt tagcactctt ggggaactct ctagtttatc
    24841 tattgagaca gtataatgcg cgattgctgg atgtgaaggg aatttttctc atgacctttg
    24901 ccctaaatgc cttgattttt gtggtcaatt tggtgacagg tggcgattac ggatttttga
    24961 caaaaccgcc attggttggg gatcacggtc tagtagctaa ttatttactt gtttcaattg
    25021 tgctggtagc tactatcagt ttgactaaga aaatcttaga attcttttta gctcaagaag
    25081 cagaaaaaat gattgcaaag gaagcttaac acagagcttt cttttttgct cttagagagt
    25141 ttttacaagc agcttataaa ataagaattt ctgaatagac aaactcaaaa aatggctggg
    25201 aaatttagga aaaaagcaag cacgattaaa ttttttgtgt tataatattt tgtgaatagc
    25261 tatgcctatg tttagctatg gaataatacg aagtgcgaaa cttggaagat agagaggaag
    25321 cgatgtaatg gctagagaag gcttttttac aggtctagat attggaacaa gctctgtcaa
    25381 ggtgcttgtg gccgagcaga gaaatggtga attaaatgta attggcgtga gtaatgccaa
    25441 aagtaaaggt gtaaaggatg gaattattgt tgatattgat gcagcagcaa ctgctatcaa
    25501 gtcagccatt tcccaagcgg aagaaaaggc aggcatttcg attaaatcag taaatgtcgg
    25561 cttgcctggt aatcttttgc aggtagaacc aactcagggg atgattccag taacatctga
    25621 tactaaggaa attacggatc aagatgttga aaatgttgtc aaatcagctt tgacaaagag
    25681 tatgacacct gaccgtgaag tcattacctt tattcctgaa gaatttattg tggatggttt
    25741 ccaagggatt cgtgacccac gtggcatgat gggggttcgc cttgaaatgc gtggtttgct
    25801 ttatacagga cctcgtacta tcttgcacaa tttgcgtaag acggttgagc gtgcaggtgt
    25861 tcaggttgaa aatgttatca tttcaccact agcaatggtt cagtctgttt tgaacgaagg
    25921 ggaacgtgaa tttggtgcta cagtgattga tatgggggca ggtcaaacga ctgtcgctac
    25981 aatccgtaat caagaactcc agttcacaca tattctccaa gaaggtggag attatgtaac
    26041 taaagatatc tccaaggttt tgaaaacctc tcgcaaatta gcggaaggct tgaaactgaa
    26101 ttacggggaa gcctatccgc ctcttgcaag caaagaaacc ttccaagtag aggttattgg
    26161 agaagtagaa gcagtcgaag tgacggaagc ctacttgtca gaaattattt ctgcacgaat
    26221 caagcacatc cttgaacaaa tcaagcaaga attagataga aggcgtctat tggacctccc
    26281 tggtggtatt gtcttaatcg gtgggaatgc cattttacca ggtatggttg agcttgctca
    26341 ggaagtcttt ggcgtccgtg tcaagcttta tgttccaaat caagttggta tccgtaatcc
    26401 agcctttgcg catgtgatta gtttatcaga atttgcgggt caattaacag aagttaatct
    26461 tttggctcag ggagcgataa aaggtgagaa tgacttaagt catcagccaa ttagttttgg
    26521 tgggatgctg caaaaaacag ctcagtttgt acaatcaacg cctgttcaac cagctcctgc
    26581 tccagaagta gagccggtgg cgcctacaga accaatggcg gatttccaac aagcttcaca
    26641 aaataaaccg aaattagcag atcgtttccg tggattgatc ggaagcatgt ttgacgaata
    26701 aagaggaaaa ataaattatg acattttcat ttgatacagc tgctgctcaa ggggcagtga
    26761 ttaaagtaat tggtgtcggt ggaggtggtg gcaatgccat caaccgtatg gtcgacgaag
    26821 gtgttacagg cgtagaattt atcgcagcaa acacagatgt acaagcattg agtagtacaa
    26881 aagctgagac tgttattcag ttgggaccta aattgactcg tggtttgggt gcaggaggtc
    26941 aacctgaggt tggtcgtaaa gccgctgaag aaagcgaaga aacactgacg gaagctatta
    27001 gtggtgccga tatggtcttc atcactgctg gtatgggagg aggctctgga actggagctg
    27061 ctcctgttat tgctcgtatc gccaaagatt taggtgcgct tacagttggt gttgtaacac
    27121 gtccctttgg ttttgaagga agtaagcgtg gacaatttgc tgtagaagga atcaatcaac
    27181 ttcgtgagca tgtagacact ctattgatta tctcaaacaa caatttgctt gaaattgttg
    27241 ataagaaaac accgcttttg gaggctctta gcgaagcgga taacgttctt cgtcaaggtg
    27301 ttcaagggat taccgatttg attaccaatc caggattgat taaccttgac tttgccgatg
    27361 tgaaaacggt aatggcaaac aaagggaatg ctcttatggg tattggtatc ggtagtggag
    27421 aagaacgtgt ggtagaagcg gcacgtaagg caatctattc accacttctt gaaacaacta
    27481 ttgacggtgc tgaggatgtt atcgtcaacg ttactggtgg tcttgactta accttgattg
    27541 aggcagaaga ggcttcacaa attgtgaacc aggcagcagg tcaaggagtg aacatctggc
    27601 tcggtacttc aattgatgaa agtatgcgtg atgaaattcg tgtaacagtt gtcgcaacgg
    27661 gtgttcgtca agaccgcgta gaaaaggttg tggctccaca agctagatct gctactaact
    27721 accgtgagac agtgaaacca gctcattcac atggctttga tcgtcatttt gatatggcag
    27781 aaacagttga attgccaaaa caaaatccac gtcgtttgga accaactcag gcatctgctt
    27841 ttggtgattg ggatcttcgc cgtgaatcga ttgttcgtac aacagattca gtcgtttctc
    27901 cagtcgagcg ctttgaagcc ccaatttcac aagatgaaga tgaattggat acacctccat
    27961 ttttcaaaaa tcgttaagta aatgaatgta aaagaaaata cagaacttgt ttttcgagaa
    28021 gttgcagagg ctagtctgag tgctcatcga gagagtggtt cggtctctgt cattgcagtt
    28081 accaagtatg tagatgtacc gacagcggaa gccttgcttc cgctaggtgt tcatcatatc
    28141 ggtgaaaatc gtgtagataa gtttctggaa aaatatgaag ctttaaaaga tcgagatgtg
    28201 acttggcatt tgattggtac cttgcaaaga cgtaaggtga aagatgtcat tcaatacgtt
    28261 gattatttcc atgcattgga ctcagtaaag ctagcagggg aaattcaaaa aagaagtgac
    28321 cgagtcatca aatgtttcct tcaagtaaat atttctaaag aagaaagcaa acacggtttt
    28381 tcgagagagg aactgctgga aatcttgcca gagttagcca gactagataa gattgaatat
    28441 gttggtttaa tgacgatggc accttttgag gctagcagtg agcagttgaa agagattttc
    28501 aaggcggccc aagatttaca aagagaaatt caagagaaac aaattccaaa tatgcctatg
    28561 accgagttaa gtatgggaat gagtcgtgat tataaagaag cgattcaatt cggttccact
    28621 tttgttcgta taggtacatc attttttaag taggagagaa ccatgtcttt aaaagataga
    28681 ttcgatagat ttatagatta ttttacggag gatgaggatt caagtctccc ttatgaaaaa
    28741 agagatgagc ctgtgtttac tccagtaaat tcttcacagg aaccggctct cccaatgaat
    28801 caaccttcac agtcggctgg tacaaaagag aacaatatca ccagacttca tgcaagacaa
    28861 caggaattgg caaatcagag tcagcgtgca acggataagg tcattataga tgttcgttat
    28921 cctagaaaat atgaggatgc aacagaaatt gttgatttat tggcaggaaa cgaaagtatc
    28981 ttgattgatt ttcagtatat gacagaggtg caggctcgtc gttgtttgga ctatttggat
    29041 ggagcttgtc atgttttagc tggaaatttg aaaaaggtag cttctaccat gtatttgttg
    29101 acaccagtta atgttattgt aaatgttgaa gatatccgtt taccagatga agatcaacag
    29161 ggtgagttcg gttttgatat gaagcgaaat agagtacgat aatgattttt ttaattcgta
    29221 tgatttataa tgcagtggat atttactccc tgattttggt agccttcgct gtcatgtctt
    29281 ggtttccagg tgcctacgaa tccagtttag gtcgttggat tgtagcgttg gtgaaaccag
    29341 tacttgctcc cttgcaacgc ctgcctttac agatagcggg tcttgattta tctgtttggg
    29401 tcgcgattgt tttggttcga tttttaggag aaaacctagt gcgttttctg gcgatgatag
    29461 gatgaataaa gggatttatc agcatttctc catagaagat cgtccatttc ttgacaaggg
    29521 aatggaatgg ataaagaagg tagaagatag ctatgctcct tttttaactc cttttatcaa
    29581 tcctcatcag gagaagctat taaagatttt ggccaaaacc tatggtcttg cttgtagcag
    29641 tagtggggaa ttcgtctcga gtgagtatgt tcgagtttta ttatacccag attatttcca
    29701 accagagttt tcagattttg aaatatctct ccaagaaatt gtgtattcca ataaatttga
    29761 acatttaacg catgctaaga ttttagggac agtcatcaat caattaggga ttgaacggaa
    29821 actttttgga gatatcctag tagatgaaga acgggcgcag attatgatta atcagcagtt
    29881 tcttcttctc tttcaagatg gactaaagaa aattggtcgt atacctgttt cgctggagga
    29941 acgtcctttc accgagaaaa tagataagct agaacagtat cgagaactgg atttatctgt
    30001 gtctagtttt cgattagatg ttcttttatc aaatgttttg aaactatcta ggaatcaagc
    30061 aaaccagttg attgaaaaga aacttgtcca agtaaattat catgtggtag acaaatcaga
    30121 ttacactgtt caagttggag acttgattag tgtgagaaaa tttggtcgct tgagattact
    30181 tcaagataag ggacaaacga aaaaagagaa gaaaaaaata accgtccagt tattattaag
    30241 taagtgagga atagaatgcc aattacatca ttagaaataa aggacaagac ttttggaact
    30301 cgattcagag gttttgatcc agaagaagtc gatgaatttt tagatattgt ggttcgtgat
    30361 tacgaagatc ttgtgcgtgc gaatcatgat aaaaatttgc gtattaagag tttagaagag
    30421 cgtttgtctt actttgatga aataaaagat tcattgagcc agtctgtatt gattgctcag
    30481 gatacagctg agagagtgaa acaggcggcg catgaacgtt caaacaatat cattcatcaa
    30541 gcagagcaag atgcgcaacg cttgttggaa gaagctaaat ataaggcaaa cgagattctt
    30601 cgtcaagcaa ctgataatgc taagaaagtc gctgttgaaa cagaagaatt gaagaacaag
    30661 agccgtgtct tccaccaacg tctcaaatct acaattgaga gtcagttggc tattgttgaa
    30721 tcttcagatt gggaagatat tctccgtcca acagctactt atcttcaaac tagtgatgaa
    30781 gcctttaaag aagtggttag cgaagtactt ggagaaccga ttccagctcc aattgaagaa
    30841 gaaccaattg atatgacacg tcagttctct caagcagaaa tggaagaatt acaagctcgt
    30901 attgaggtag ccgataaaga attgtctgaa tttgaagctc agattaaaca ggaagtggaa
    30961 actccaactc ctgtagtgag tcctcaagtt gaagaagagc ctctgctcat ccagttggcc
    31021 caatgtatga agaaccagaa gtagctccag tgcatccgac aggtccaaca ccagctacag
    31081 aaactgttga ttcagcacca ggatttgaag caccgcaaga atccgttaca attttataag
    31141 aaacgatgtg agaacaatat cttatcctta tatttccagc gagcaggaga tggtgtgagt
    31201 cctgcattcc ctaacgataa gattatcctc tcaaaaactc aagtctgaag ctagtaagat
    31261 ttgacgtttc ccacgttacg ggataagagg gagaaagact aaatcttttt ccgaataaag
    31321 gtggtaccac gattttcgtc ctttttggaa gtcgtggttt ttaatttgtt attatttata
    31381 aaggagatac catgaaactc aaagataccc ttaatcttgg gaaaacagct ttcccaatgc
    31441 gtgcaggcct tccgaccaaa gagccagttt ggcaaaagga atgggaagat gcaaaacttt
    31501 accaacgtcg tcaagaacta aatgaaggga aaccgcattt tgtcttgcat gatggccctc
    31561 catacgcaaa cggaaatatc cacgttggac atgccatgaa tcatatctca aaagatatca
    31621 tcattcgttc taagtctatg tcaggtttta acgcacctta tatcccaggt tgggatactc
    31681 atggtctgcc aatcgagcaa gttttggcaa agcaaggtgt gaagcgcaaa gaaatggact
    31741 tggttgagta cttgaaactc tgtcgtgagt acgctctttc tcaagtttat aaacaacgtg
    31801 atgatttcaa acgcttgggg atgtcaggtg actgggaaaa tttatatgtg actttaactc
    31861 cagactatga agcagcccaa atccgtgtct ttggtgagat ggctaataag ggttatatct
    31921 accgtggtgc caagccagtt tactggtcat ggtcatctga gtcagccctt gccgaagcgg
    31981 aaattgaata ccatgatttg gtttcgactt cactttatta tgccaataag gtaaaagatg
    32041 gcaaaggtat cctagataca gatacttata tcgtagtctg gacaacaact ccatttacca
    32101 tcacagcttc tcgtggtttg actgttggtg cagatattga ttacgttttg gttcaacctg
    32161 ctagtgaaac tcgtaagttt gtcgttgctg cagaattgtt gactagcttg tctgagaaat
    32221 ttggctgggc tgatgttcaa gtgttggcaa cttaccgtgg tcaagaactc aaccacatcg
    32281 taacagaaca cccatgggat acagctgtag atgagttggt aatccttggt gatcacgtta
    32341 ccactgactc tggtacaggt atcgtccata cagcccctgg ttttggtgag gacgactaca
    32401 atgttggtat tgctaatggt cttgaagtcg cagtgactgt tgacgaacgc ggtatcatga
    32461 tggcgaatgc tggtcctgag tttgaaggtc agttctacga caaggttgta ccaactgtta
    32521 ttgaaaaact tggtaacctc cttctagccc aagaagaaat ctctcactca tacccatttg
    32581 actggcgtac taagaaacca atcatctggc gtgcagttcc acaatggttt gcctcagttt
    32641 caaaattccg ccaagaaatc ttggatgcta ttgataaagt taaattccat acagaatggg
    32701 gtaaagtacg tctatataat atgattcgtg accgtggtga ctgggttatc tctcgtcaac
    32761 gtgcttgggg tgttccactt ccaatcttct atgcagaaga cggtacagct atcatgacag
    32821 ctgagactat tgaacatgtg gcgcaactct ttgaagtaca tggttcaagc atttggtggg
    32881 aacgtgatgc caaagacctc ttaccagaag gatttacaca tccaggttct ccaaatgggg
    32941 agttcaagaa agaaacagat attatggatg tatggtttga ctcaggttca tcatggaatg
    33001 gtgttttggt aaatcgtcca aatctgactt atccagctga cctttacctt gaaggttctg
    33061 accaataccg tggttggttc aactcatcac tcattacatc tgttgccaac catggcgtag
    33121 caccttacaa acaaatcttg tcacaaggtt ttacccttga tggtaaaggt gagaagatgt
    33181 ctaaatcact tggaaatacc attgctccaa gtgatgttga aaaacaattt ggtgcagaaa
    33241 tcttgcgtct ctgggtaaca agtgttgact caagcaatga cgtgcgtatc tctatggata
    33301 ttttgagcca agtttctgaa acttaccgta agattcgtaa cactcttcgt ttcttgattg
    33361 ccaatacatc tgactttaac ccagctcaag atgtagtcgc ttacgatgaa cttcgttcag
    33421 ttgataagta catgactatt cgctttaacc agcttgtcaa gaccattcgt gatgcctatg
    33481 cggactttga attcttgaca atctacaagg ccttggtgaa ctttatcaac gttgacttgt
    33541 cagccttcta ccttgatttt gccaaagatg ttgtttacat tgaaggtgcc aaatcattgg
    33601 aacgccgtca aatgcagact gttttctatg acattcttgt caaaatcacc aaactcttga
    33661 caccaatcct tcctcacact gcggaagaaa tctggtcata tcttgagttt gaagctgaag
    33721 acttcgttca attgtcagaa ttgccagaag ctcaaacttt tgctaatcaa gaagaagtct
    33781 tggatacatg ggcagccttc atggacttcc gtggacaagc tcaaaaagcc ttggaagaag
    33841 ctcgtaatgc aaaagttatc gggaaatcgc ttgaagcaca cttgacagtt tatccaaatg
    33901 aagttgtgaa aactctactc gaagcagtaa acagcaatgt ggctcaactt ttgatcgtgt
    33961 cagacttgac catcgcagaa gggccagctc cagaagctgc ccttagcttc gaagatgtag
    34021 ccttcacagt tgaacgcgct gcaggtcaag tatgtgaccg ttgccgtcgt atcgacccaa
    34081 caacagcaga acgcagctac caggcagtca tctgtgacca ctgtgcaagc atcgtagaag
    34141 aaaactttgc ggaagcagtc gcagaaggat ttgaagagaa ataaggttga aaagtctagg
    34201 caaaattcaa tttgagaaga aaagacaact aattttatag tctattaaac gcattgtatc
    34261 acgtttttga atacctgata tgatgcgttt tttatttatt ttaaaaattt gcgaggtatg
    34321 actttttata ctcaacaaga atcaaagaga aacttagcaa gctaacagta gtaagataaa
    34381 ataggaat
//
LOCUS       DAVMGZ010000009        30179 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000009 DAVMGZ010000000
VERSION     DAVMGZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30179
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.009"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            9..89
                     /locus_tag="VYQ93_000342"
     tRNA            9..89
                     /locus_tag="VYQ93_000342"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Tyr,seq:gta)
     gene            94..164
                     /locus_tag="VYQ93_000343"
     tRNA            94..164
                     /locus_tag="VYQ93_000343"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Trp,seq:cca)
     gene            179..251
                     /locus_tag="VYQ93_000344"
     tRNA            179..251
                     /locus_tag="VYQ93_000344"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:His,seq:gtg)
     gene            265..336
                     /locus_tag="VYQ93_000345"
     tRNA            265..336
                     /locus_tag="VYQ93_000345"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:297..299,aa:Gln,seq:ttg)
     gene            346..429
                     /locus_tag="VYQ93_000346"
     tRNA            346..429
                     /locus_tag="VYQ93_000346"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:380..382,aa:Leu,seq:caa)
     gene            536..1813
                     /locus_tag="VYQ93_000347"
     CDS             536..1813
                     /locus_tag="VYQ93_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV3335093.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1889..3373
                     /locus_tag="VYQ93_000348"
     CDS             1889..3373
                     /locus_tag="VYQ93_000348"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV3335094.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3434..4714
                     /locus_tag="VYQ93_000349"
     CDS             3434..4714
                     /locus_tag="VYQ93_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV3335095.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4718..5338
                     /locus_tag="VYQ93_000350"
     CDS             4718..5338
                     /locus_tag="VYQ93_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV3335096.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5504..6616
                     /locus_tag="VYQ93_000351"
     CDS             5504..6616
                     /locus_tag="VYQ93_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV3335097.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            6939..7364
                     /locus_tag="VYQ93_000352"
     CDS             6939..7364
                     /locus_tag="VYQ93_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV3335098.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7375..7734
                     /locus_tag="VYQ93_000353"
     CDS             7375..7734
                     /locus_tag="VYQ93_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV3335099.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            7841..8485
                     /gene="pcp"
                     /locus_tag="VYQ93_000354"
     CDS             7841..8485
                     /gene="pcp"
                     /locus_tag="VYQ93_000354"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV3335100.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(8513..9370)
                     /locus_tag="VYQ93_000355"
     CDS             complement(8513..9370)
                     /locus_tag="VYQ93_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV3335101.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9500..10642
                     /gene="tgt"
                     /locus_tag="VYQ93_000356"
     CDS             9500..10642
                     /gene="tgt"
                     /locus_tag="VYQ93_000356"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV3335102.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10740..12557
                     /locus_tag="VYQ93_000357"
     CDS             10740..12557
                     /locus_tag="VYQ93_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV3335103.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12710..13861
                     /gene="nagA"
                     /locus_tag="VYQ93_000358"
     CDS             12710..13861
                     /gene="nagA"
                     /locus_tag="VYQ93_000358"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV3335104.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14024..15082
                     /locus_tag="VYQ93_000359"
     CDS             14024..15082
                     /locus_tag="VYQ93_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV3335105.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15233..15598
                     /locus_tag="VYQ93_000360"
     CDS             15233..15598
                     /locus_tag="VYQ93_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV3335106.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15673..16614
                     /locus_tag="VYQ93_000361"
     CDS             15673..16614
                     /locus_tag="VYQ93_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV3335107.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGINVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16562..17578
                     /locus_tag="VYQ93_000362"
     CDS             16562..17578
                     /locus_tag="VYQ93_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV3335108.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17580..17906
                     /locus_tag="VYQ93_000363"
     CDS             17580..17906
                     /locus_tag="VYQ93_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV3335109.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            17899..18303
                     /locus_tag="VYQ93_000364"
     CDS             17899..18303
                     /locus_tag="VYQ93_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV3335110.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18308..18568
                     /locus_tag="VYQ93_000365"
     CDS             18308..18568
                     /locus_tag="VYQ93_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335111.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18531..18992
                     /locus_tag="VYQ93_000366"
     CDS             18531..18992
                     /locus_tag="VYQ93_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV3335112.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            18970..19416
                     /locus_tag="VYQ93_000367"
     CDS             18970..19416
                     /locus_tag="VYQ93_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV3335113.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNALNPE"
     gene            19519..20106
                     /locus_tag="VYQ93_000368"
     CDS             19519..20106
                     /locus_tag="VYQ93_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV3335114.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKVILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQARLKVEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLIVAQKSLA"
     gene            20167..21120
                     /locus_tag="VYQ93_000369"
     CDS             20167..21120
                     /locus_tag="VYQ93_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV3335115.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSNLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21171..22361
                     /locus_tag="VYQ93_000370"
     CDS             21171..22361
                     /locus_tag="VYQ93_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV3335116.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22510..22881
                     /gene="rnpA"
                     /locus_tag="VYQ93_000371"
     CDS             22510..22881
                     /gene="rnpA"
                     /locus_tag="VYQ93_000371"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV3335117.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVN"
     gene            22856..23680
                     /locus_tag="VYQ93_000372"
     CDS             22856..23680
                     /locus_tag="VYQ93_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV3335118.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23699..24685
                     /locus_tag="VYQ93_000373"
     CDS             23699..24685
                     /locus_tag="VYQ93_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV3335119.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24736..25359
                     /locus_tag="VYQ93_000374"
     CDS             24736..25359
                     /locus_tag="VYQ93_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV3335120.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            25490..26947
                     /locus_tag="VYQ93_000375"
     CDS             25490..26947
                     /locus_tag="VYQ93_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV3335121.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLAAYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            26970..27251
                     /locus_tag="VYQ93_000376"
     CDS             26970..27251
                     /locus_tag="VYQ93_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3335122.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQRDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27327..27812
                     /locus_tag="VYQ93_000377"
     CDS             27327..27812
                     /locus_tag="VYQ93_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3335123.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            27829..28494
                     /locus_tag="VYQ93_000378"
     CDS             27829..28494
                     /locus_tag="VYQ93_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV3335124.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            28498..29361
                     /locus_tag="VYQ93_000379"
     CDS             28498..29361
                     /locus_tag="VYQ93_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV3335125.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29363..30067
                     /locus_tag="VYQ93_000380"
     CDS             29363..30067
                     /locus_tag="VYQ93_000380"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV3335126.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYRAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
BASE COUNT         9047 a         5341 c         6940 g         8851 t
ORIGIN      
        1 tatattttgg aagggtagcg aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg
       61 gttcgggggt tcgaatccct ccccttccat tttacgggca tagtttaaag gtagaactaa
      121 ggtctccaaa accttcagtg tgggttcaat tcctactgcc cgtgttaata gaattatggc
      181 gggtgtggtg aagtggttaa cacaccagat tgtggctctg gcatgcgtgg gttcgatccc
      241 catcactcgc ctattttata ttattggggt atagccaagc ggtaaggcaa gggactttga
      301 ctccctcatg cgttggttcg aatccagcta ccccagttac tatttgccgg cgtggcggaa
      361 ttggcagacg cgctggactc aaaatccagt gtccgcaagg acgtgccggt tcgaccccgg
      421 ccgccggtat agtatagtgt taggaacgtt gttattcttc gttccttttt tatattattt
      481 ttggtataat tatagttatt caaattttat ttagattaag aaagtgtagg ggagtatgtc
      541 ttgttctatc gatttattaa aacatcggta tttgaaaaat attaaagaaa atcctgaatt
      601 gtttgtcgga attgagttgg agtatcctgt tgcaagttta gaaggggatg ctacagatgt
      661 tgaagttatg aaggatctat ttcattattt agtttctact ttggatctca ccgtagcaaa
      721 ggtagatgat tttggcaatc tgatccagtt agtagatccg ataagtcagg atgctatttt
      781 atttgaagtt tcctatacaa cgattgagtt tgcatttggt aaggctgaaa cgattcaaga
      841 ggtcgaaaat cgtttcaata attatatgaa tgtaattcag agaaagttag ctgaatcaaa
      901 tcatgctatt gttggctgtg gtatccatcc caactgggat aaaaatgaga attgtccagt
      961 ggcttatcca cgctatcaga tgttgatgga ttatttgaat ttgagtagaa atattattaa
     1021 atcagattta catcatttcc ctgaatatgg tacttttatc tgtgggagcc aggttcagct
     1081 ggatatttca aaaaccaact acttacgggt gattaatgct tttactcaaa ttgaagcggc
     1141 taaggcttat ttatttgcaa actctgaatt ttcgggtgcg gattgggata cgaaaatttc
     1201 aagggatatt ttctgggaag aatctatgca tggtatctat ccagagaatg ttggggtcaa
     1261 tgctagactc cttaatgatg aagctgattt ttttgactat ctaaatcatt ctgcgatttt
     1321 tactgcggaa cgtgatgggc agacctatta tttttatcct attcaggctg gggactattt
     1381 ggctacgtcc gaaatccaag catttgctct gaatggggat gaggttatta tttaccccca
     1441 agagaaggat tttgaaactc atcgtagtta ccagtaccaa gatttaacga ctcgaggaac
     1501 agttgagttt cgtagtgtgt gtacacagcc acttgatagg acttttgctt ctgcagcttt
     1561 tcacttggga ttattggtta atttagacaa gttagaagct tacttagaaa cagcaccttt
     1621 ctttaaagta tttggttatg attacaagtc tttaaggaga caattttcta agaaaaatct
     1681 tacagatgag gaagaaacta cgattattga attttccaaa gacttactcc tactagctga
     1741 ggagggacta gtggtgagaa ataaggaaga aatgacctat ttacagcctt tgagagaaga
     1801 attgagccta taatttctct tataaaggga gaattttctg aaaaatcatg atataatgga
     1861 cgagactata gataaaggat agagagtaat gacattagtt tatcaatcaa cgcgtgatgc
     1921 caacaataca gtaactgcca gccaagcaat tttgcaaggt ttggcgacgg acggcggttt
     1981 gtttacaccg gatacttatc caaaggtaga tttgaacttt gacaaattga aagatgcttc
     2041 ttaccaggaa gttgctaagc tagttttgtc agcattttta gatgacttta cagttgagga
     2101 gttggactac tgtatcaaca atgcctacga tagcaaattt gatactccag ctattgcacc
     2161 attagtgaaa ttagatgggc aatacaattt ggaacttttc catggttcaa cgattgcctt
     2221 taaggatatg gccttgtcta ttttgccata ctttatgacg actgctgcta agaaacatgg
     2281 tttggagaac aagattgtta tcttgacagc gacatctggt gacacgggga aagctgctat
     2341 ggcggggttt gcgaatgtgc ctggtactga gattatcgtc ttttatccaa aggatggtgt
     2401 cagcaagatt caagagttac aaatgaccac tcagactggc gacaatactc atgttattgc
     2461 tattgatggt aactttgacg atgcgcaaac aaatgtgaag cacatgttta acgacgtggc
     2521 tcttcgtgaa aaattgacta ccaacaagtt gcaattttca tcagctaact ctatgaacat
     2581 tggtcgtctg gtgccacaaa ttgtttatta tgtttatgct tacgctcaat tggttaagac
     2641 tggtgaaatt gtagctggtg aaaaggttaa cttcacagta ccaacaggaa actttggaaa
     2701 tatcttggct gccttttatg ccaaacaaat tggtttgcca gttggtaaat taatctgtgc
     2761 ttcaaatgac aacaatgttt tgacagactt ctttaaaaca cgtgtctatg acaaaaaacg
     2821 tgagtttaag gtaacaacca gcccatctat ggatatcttg gtatcttcaa acttggagcg
     2881 cttgattttc catcttttgg gaaataatgc tgaaaagaca actgaactta tgaatgcctt
     2941 gaacacgcaa ggacaatata agttgacaga ctttgatgca gagattttgg acctctttgc
     3001 agctgaatat gcgactgagg aagaaacggc agcagagatc aagcgtgttt gtgagttaga
     3061 ttcttatatc gaggaccctc atacagctgt tgcttcagca gtttataaaa aataccaatc
     3121 ggccactgga gatgtaacta agacagtgat tgcttcaaca gctagtccat acaagttccc
     3181 agtagttgca gtagaagctg taactggaaa agcaggttta acagactttg aagccttggc
     3241 tcaattacat gaaatctcag gcgttgcagt gccaccagca gttgatgggc ttgaaatagc
     3301 tccaattcgt cacaagacaa cagtggcagc tgctgacatg caagcagcgg ttgaggctta
     3361 tttaggactt taagacagag ggagcaaact cggttgggaa accaactgag tttcttttca
     3421 tcaggaggag agattgttta agaaaaataa agacattctt aatattgcat tgccagctat
     3481 gggtgaaaac tttttgcaga tgctaatggg aatggtggac agttatttgg ttgctcattt
     3541 aggattgata gctatttcag gggtttcagt agctggtaat attatcacca tttatcaggc
     3601 gattttcatc gctctgggag ctgctatttc cagtgttatt tcaaaaagca tagggcagaa
     3661 agaccagtcg aagttggcct atcatgtgac tgaggcgttg aagattacct tactattaag
     3721 tttcctttta ggatttttgt ccatcttcgc tgggaaagag atgataggac ttttggggac
     3781 ggagagggat gtagctgaga gtggtggact gtatctatct ttggtaggcg gatcgattgt
     3841 tctcttaggt ttaatgacta gtctaggagc cttgattcgt gcaacgcata atccacgtct
     3901 gcctctctat gttagttttt tatccaatgc cttgaatatt cttttttcaa gtctagctat
     3961 ttttgttctg gatatgggga tagctggtgt tgcttggggg acaattgtgt ctcgtttggt
     4021 tggtcttgtg attttgtggt cacaattaaa actgccttat gggaagccaa cttttggttt
     4081 agataaggaa ctgttgacct tggctttacc agcagctgga gagcgactta tgatgagggc
     4141 tggagatgta gtgatcattg ccttggtcgt ttcttttggg acggaggcag ttgctgggaa
     4201 tgcaatcgga gaagtcttga cccagtttaa ctatatgcct gcctttggcg tcgctacggc
     4261 aacggtcatg ctgttggccc gagcagttgg agaggatgat tggaaaagag ttgctagttt
     4321 gagtaaacaa accttttggc tttctctgtt cctcatgttg cccctgtcct ttagtatata
     4381 tgtcttgggt gtaccattaa ctcatctcta tacgactgat tctctagcgg tggaggctag
     4441 tgttctagtg acactgtttt cactacttgg gacccctatg acgacaggaa cagtcatcta
     4501 tacggcagtc tggcagggat taggaaatgc acgcctccct ttttatgcga caagtatagg
     4561 aatgtggtgt atccgcattg ggacaggata tctgatgggg attgtgcttg gttggggctt
     4621 gcctggtatt tgggcagggt ctctcttgga taatggtttt cgctggttat ttctacgcta
     4681 tcgttaccag cgctatatga gcttgaaagg ataggaaatg caaaaaacag cttttatttg
     4741 ggatttagac gggactttat tggactctta cgaagcgatt ttatcaggga ttgaggagac
     4801 ttttgctcag ttttctattc cttatgataa ggagaaggtg agagagttta tcttcaagta
     4861 ttcggtgcaa gatttgcttg tgcgggtggc agaagataga aatctggatg ttgaggtgct
     4921 aaatcaggtg cgtgcccaga gtctggctga gaagaatgct caggtagttt tgatgccagg
     4981 tgcgcgtgag gtgctagctt gggcagacga atcaggaatt cagcagttta tatatactca
     5041 taaggggaac aacgctttta ccattctcaa ggacttgggg gtggaatcct attttacaga
     5101 gattttaacc agtcagagtg gctttgtgcg gaagccaagt ccagaagcgg ctacctatct
     5161 gctagataag tatcagttga attctgataa tacttattat ataggggatc ggactctgga
     5221 tgtggaattt gcccagaata gtgggattca aagcatcaac tttttagagt ctacttatga
     5281 agggaatcac aggattcaag cgttagcaga tatttcccgt atttttgaga ctaagtgata
     5341 aaaagattgt gtcagttttg tgacagagac ctaacaaact atttcaagta acctagtttg
     5401 ttacaaggaa tagacagttc tgttaaatag gcccgagagg gctttttttc tacatttttt
     5461 gtgttatgat agacaggtac tcatttgaaa ggaatttgaa agaatgaaga aaagaatgtt
     5521 attagcgtca acagtagcct tgtcatttgc cccagtattg gcaactcaag cagaagaagt
     5581 tctttggact gcacgtagtg ttgagcaaat ccaaaacgat ttgactaaaa cggacaacaa
     5641 aacaagttat accgtacagt atggtgatac tttgagcacc attgcagaag ccttgggtgt
     5701 agatgtaaca gtgcttgcga atctgaacaa aatcactaat atggacttga ttttcccaga
     5761 aactgttttg acaacgactg tcaatgaagc agaagaagta acagaagttg aaatccaaac
     5821 acctcaagca gactctagtg aagaagtgac aactgcgaca gcagatttga ccactaatca
     5881 agtgaccgtt gatgatcaaa ctgttcaggt tgcagacctt tctcaaccaa ttgcagaagt
     5941 tacaaagaca gtgattgctt ctgaagaagt ggcaccatct acgggcactt ctgtcccaga
     6001 ggagcaaacg accgaaacaa ctcgcccagt tgaagaagca actcctcagg aaacgactcc
     6061 agctgagaag caggaaacac aagcaagccc tcaagctgca tcagcagtgg aagtaactac
     6121 aacaagttca gaagcaaaag aagtagcatc atcaaatgga gctacagcag cagtttctac
     6181 ttatcaacca gaagagacga aaataatttc aacaacttac gaggctccag ctgcgcccga
     6241 ttatgctgga cttgcagtag caaaatctga aaatgcaggt cttcaaccac aaacagctgc
     6301 ctttaaagaa gaaattgcta acttgtttgg cattacatcc tttagtggtt atcgtccagg
     6361 agacagtgga gatcacggaa aaggtttggc tatcgacttt atggtaccag aacgttcaga
     6421 attaggggat aagattgcgg aatatgctat tcaaaatatg gccagccgtg gcattagtta
     6481 catcatctgg aaacaacgtt tctatgctcc attcgatagc aaatatgggc cagctaacac
     6541 ttggaaccca atgccagacc gtggtagtgt gacagaaaat cactatgatc acgttcacgt
     6601 ttcaatgaat ggataaaccc gacttgataa catcattttg acgaatgaga tctagctttc
     6661 gtgatggaaa gcgattctcg ttcgtttttt ctttgtcata ctcttcgaaa atctcttcaa
     6721 accacgtcag ttttatctga aacttcaaag ctgtgctttg agcaacctgc gactagcttc
     6781 ctagtttgct ttttgatttt cattgagtat caatttgaat ggaaaatgga aagttatcat
     6841 cttgtaatga gttaagcaac attcttgcaa tctattttac tttatatcac aattaattag
     6901 tcaaatattg ataaatcaat aaaaagagag gggaagaaat gctagagatt caagatttac
     6961 tgtatcaact ccgcttgtct gagcaagcga gtacgcaatt gtttgaaaaa aggcttggga
     7021 ttagtttgac acggtatcag attttactgt ttttgctgga gcattctcct tgtaaccaaa
     7081 tggcggttca ggagcgtttg aaaattgatc aggctgcttt gacacggcat ttcaaaattt
     7141 tggaaacgga aggtttggtg gagcgtcatc gtaatcctga aaatcagcgg gaagtgttgg
     7201 tagaggctgc gaagtatgcc aaggagcagt tagtggtgaa tccccctctg caacatatca
     7261 gggttaagga agagatagaa agtatcttaa cagagtttga gagaacagaa ctcagccgtt
     7321 tattaaataa attggttttg ggtattgaaa atatagaaat ttaaggagaa atagatgtca
     7381 attattttaa caacgatcgt tgctttggag catttttaca ttttttattt ggaaagtatt
     7441 gccacgcaat cagatgcgac tagtcgtgta tttaatatgg aaaaggaaga attggctcat
     7501 ccgtcagtaa gttcattgtt caaaaatcaa ggaatttata aggctctgct aggagtcttt
     7561 ctcttgtatg tcatttattt ctcacagaat ttagaaattg tgactatttt tgtcttattt
     7621 gtgattggtg ctgcgactta cggctcttta acagcggata aaaaaattat tttgaaacaa
     7681 ggtggatcag ctattttggc cttgattagt attttactct ttaaatacac ttgaaggtcg
     7741 attctaatct cgctaatcct ttttaatcca gaataaggga aatatgttat acttgttttt
     7801 aagaaaaaag tctcattgaa ttggttttga ggagttagaa atgaaagtat tagtgacagg
     7861 ttttgagccc tttggagggg aaaagggcaa tccagctttg gaggccatta aaggtttacc
     7921 agctgaaatc catggtgctg aggtccgttg gctagaggtg ccgacagttt ttcacaaatc
     7981 tgctcaagta ttggaagaag agatgaatcg ttatcaacct gactttgtcc tttgtattgg
     8041 gcaagctggt ggaagaacta gtttgacacc tgaacgagtg accattaatc aagacgatgc
     8101 atgcatttct gataacgaag ataatcaacc gattgaccgt cccattcgcc cagatggtgc
     8161 ttcggcctac tttagtagtt tgccgattaa agcgatggtt caagctataa aaaaagaggg
     8221 cttaccggcc tctgtttcca atacggcagg gacttttgtc tgcagccatt tgatgtatca
     8281 ggctctctat ttggtagaaa agaaatctcc atatgttaag gcaggtttta tgcatattcc
     8341 ttatatgatg gaacaggtgg tgaacagacc gactactcca gctatgagtt tagtggatat
     8401 tcggcgaggg atagaagcag caatcggcgc tataatagaa catggagatc aggaactcaa
     8461 gttggtaggc ggagaaactc attgatagaa aaaagcttga ggggaaaaac cttcaagctt
     8521 ttggacgttt tcgggccaat actgctcggt aaaacataat tttagtgcat tggatataag
     8581 gtaggagtga aaaactagca atgccaaagg taatccaatt gaggaagtac caaggaagaa
     8641 gctgtaaatc taggacaaag tgctggaact tgtagccctt cataaaggaa cggctagttt
     8701 ttaggattcg tcttggtggg acctgtccta ggtctagact ataacagaga agaaattcca
     8761 cctgtgaata ggcataatac tgtggaatat agaggatatt tcctacaatg atcaagatga
     8821 gacttgcaag aaagtagagt ccaaagacca tgaggaaacg ctcggtttca actgatgaga
     8881 gatctagatt tggaaactca ggatgtaggg tgacgaattt tttggctaaa aagctactat
     8941 aaaagaggag gtaaatccca agtaaattag ggatactcca taaaaagaga tagaaacgtt
     9001 tgagaagtag ggtcaaaaag gtttgagaaa agcgctcctc atcaaagaga gctaggctgt
     9061 tttttacaga tggctccgtt ttagaatctt tcatgagtgt cagtgttgca tagacggaac
     9121 tggtcaaaag aatagtcccg ataaaggaga ctagtagagg aaagaggtag gtttgaagta
     9181 tttggccaag tatgctgaaa aatggctgtt ctaaaacagt cccgtggatc cgagataagg
     9241 gattaagaaa accagataag atgaccagca tactgggaag gatatagagg agaaagagac
     9301 ggggggtgtc agcctgaaaa tgttttgact cctgacgaat tgtttttaaa tcaatttttg
     9361 gatagttcat tctcttatta taccatagtt cttatacata gttcgtgaca gttcctactt
     9421 tttttgataa aatcatacag tgtgtccttg ggcacactgt atgaactggg actgtctttc
     9481 ccagcttcgg aggtaaaaaa tgtcagattc accaatcaaa tatcgtttga ttaagaaaga
     9541 aaaacacaca ggagctcgtc tgggagaaat catcactccc cacggtacct ttccgacacc
     9601 tatgtttatg ccagttggga cacaagccac tgtcaaaact cagtcacctg aagaattgaa
     9661 ggagatgggt tcgggaatta tcctatcaaa cacctatcat ctctggcttc gccctggaga
     9721 tgaactcatt gcacgcgctg gtggtctcca caagttcatg aattgggacc agcctatctt
     9781 gacagatagt ggtggttttc aggtttattc tttagcagat agccgtaata tcacagaaga
     9841 aggagtaacc tttaaaaatc atctaaatgg ttctaagatg ttcctatccc cagaaaaagc
     9901 catctctatt cagaataatc tgggttcaga catcatgatg tcctttgatg aatgtcctca
     9961 gttttatcaa ccttatgact acgttaagaa atcgatcgag cgtaccagcc gttgggctga
    10021 gcgtggtttg aaggctcacc gtcgtccaca tgaccaaggt ttgtttggaa ttgtgcaagg
    10081 tgcaggattt gaagaccttc gccgccaatc agctcatgat cttgtcagca tggatttctc
    10141 aggctactct atcggtggtt tggcagtggg agaaacccat gaagagatga atgcggtctt
    10201 ggactttaca actcaactgc tacctgaaaa taaacctcgt tatctgatgg gtgtgggagc
    10261 gccagatagc ttgatcgatg gggtcattcg tggggtggat atgtttgact gtgtcttacc
    10321 gactcgaatt gctcgtaacg ggacttgtat gaccagtcaa ggacgtttgg ttgtgaaaaa
    10381 tgcccagttt gctgaggact ttacgccact ggatcctgag tgtgactgct acacatgtaa
    10441 taactataca cgcgcttacc ttcgtcacct gctcaaggct gatgaaacct ttggtatccg
    10501 cttgactagc taccacaatc tttacttctt gcttaacctg atgaagcaag tgcgacaagc
    10561 catcatggat gacaatctct tggaattccg tgagtatttt gtggaaaaat atggctataa
    10621 taagtcagga cgtaatttct aaaatggaat tgatataaaa aaatcctaag ttttctctta
    10681 ggatttttct tctttttttg atagaataaa gtgtacaatg aaaggaagaa taaactcgta
    10741 tgcgcattaa atggttttcc ttgattagga ttataggttt acttttggta ctcttgtatc
    10801 acttctttca gacgatcttt cctggaggat ttttcggggt agatgtcttt ttcacatttt
    10861 caggtttcct gattacagct ctactcattg aagaattttc taagaacaat gagattgatt
    10921 tgataggatt ttttagaaga cgcttttatc ggattgtgcc acctgtggtt ttgatggtct
    10981 tggtgaccat gccttttact ttcttggttc gccaagacta tgttgctgga attggtggcc
    11041 agattgcggg cgtcttaggc tttatgacca acttctatga actcctaaca ggtgggagtt
    11101 atgaatctca gttcattcct catttgtttg ttcataattg gagtttggca gttgaggttc
    11161 actactatat tctttgggga ttggcagttt ggttcttatc caaacaagct aaatcaaatg
    11221 gtcagttgaa ggggatggtc tttctcttat ctgctgctgc cttcttgatc agtttcttct
    11281 ccatgtttat tggtagtttt ctagtgacct cttattcctc tgtttatttc tccagtttaa
    11341 ctcatgtcta tccattcttt ttgggaagta tgttagcaac tattgtaggt gttcgtcaga
    11401 cgacttccct cgtcaagcag ttggataaaa tctgggattt acgaaagact ttggtagttt
    11461 ttggaggagg ctttggtttc ctagttcttt tgactttctt tgtcaaattc acttatcttt
    11521 ttgcctatct tatcggcttc ttgcttgcca gtcttgcagc tcttgccatg attctggcgg
    11581 cgcgtgtctt acatgaaaag acacatcata tacaggagcc gaagattatc agctttttag
    11641 cggatactag ctatgcggtt tatcttttcc attggccttt ctatatcatt ttctcacagt
    11701 tgacatcaaa tcttcttgct gtattactga ctctgatttg ttcttatggc tttgccagtc
    11761 tgtcattcta tgtattggaa ccttggattg caggcaagaa cacacctatt ttacaaactc
    11821 ttcgtcccct gccttatatt cacgcaattc ttgtagcagg tacaggaatc ttgaccatca
    11881 ttgtctgcac ggtgaccttg ttggcaccac aagtgggagc gtttgagaca gacttgactg
    11941 tcaatggctt gaagcaagct gcaacaaata ttggccagac caaggtgatg gcagaacggg
    12001 cagatgcaaa cagtttggga attgctgatg gcactatgtt aattggtgac tcagtggctt
    12061 taagggcaaa tacagcacta cagacagctc ttcctggagc acagattaac gcgcaggtca
    12121 gcgtaacaac caagaccgca aatgaaatca tgctaaataa tagccagaat aaatttttac
    12181 ctaagacggt ggtcattgcg actggggtaa ataatcctga gaattacaag gatgactggg
    12241 acagtatcgt gaaaaatctt cctaagggac accatatgat tttggtgact ccttatgagg
    12301 gagataagac aaaagagacc tatgccatcg ttgagaaggc tgctgcctat atgagagaat
    12361 tggcagagaa gacaccttac attacgatag cagattggaa tcaagttgcg aaagagcatc
    12421 cagaaatttg ggctggaaca gaccaggttc atttcgggag tgagagtagc actatcgaag
    12481 caggagcaaa attgtatgca gatacgattg ccacagcttt gcagacagct caagacaagc
    12541 cggttaaatc aaaataactt gtattaaaaa agagaagagt tggagaaatc cagctctttt
    12601 aaaatatctc caaaaaatag gtctatacca tttacaaata aaaaagaaag gtttataatg
    12661 taattgacat aataaattgt agaatcaatc ttttaaggag gttaacatta tgcctaacta
    12721 tattaaagcg gatcagtttt tctacccaca cggagttcgt cgaggtggtt acttggaact
    12781 tgtggacggc aagtttggga aacatgtaga gcagattcct gaaggggctg aggtgattga
    12841 ctatacaggt tatagcattg ccccaggtct tgtggatact catattcatg gatatgcagg
    12901 tgtagatgtg atggacaaca acattgaagg tacattgcat actatgagtg aaggacttct
    12961 tagtaccggt gttaccagtt tcttacccac aactttaaca gccacttatg agcaattgct
    13021 tgcagtcact gaaaatcttg gaaaccatta taaagaagca acaggtgcta agattcgtgg
    13081 gatttattat gaaggtccat atttcacaga aacttttaag ggggcacaaa atccaactta
    13141 tatgagagac ccgggtgttg aggagtttca ttcttggcaa aaagcggcaa atggcttgct
    13201 taataaaatt gcccttgcac cagaacgtga tggggtggaa gactttgttc gtacagttac
    13261 gggcgaaggt gtgacggttg ctcttggaca ttcaaacgcg acttttgatg aagccaaaaa
    13321 agcagtcgat gctggagcga gtgtttgggt gcatgcctac aatggaatgc gtgggttgac
    13381 tcaccgtgaa ttgggtatgg ttggagccat gtaccaattg ccacatacct atgcagagtt
    13441 gatctgtgat ggtcaccacg tagatccaaa ggcttgcgaa attcttatca aacaaaaagg
    13501 aacagaaaac atcgctctta tcacagactg tatgacagct gggggattgg aagacggaga
    13561 ttatatgttg ggagaattcc cagttgtcgt tgcaaatgga actgcacgcc tcaaatcgac
    13621 aggtaacttg gcaggttcta tcctcaaact caaagatggt ttgaagaatg tggtcgaatg
    13681 gggaattgcg aatccgcatg aagcagtcat gatggccagc ttcaacccag ctaaatccgt
    13741 tcacatcgat gacgtctgtg gccaaatccg tgaaggctac gacgctgact tcatcgtatt
    13801 agataaagat ttggaattgg tagcaaccta tctagatggc gtaaaacgtt atcaagcata
    13861 agagagaagg cagaagttag agactagctt ctgctttttt atacagggta ctttactggt
    13921 aaataaaaag ttaaaaaaat cacaaaaaaa gcttgaagaa acaaagaaaa aggcttacaa
    13981 ttgaactata aatattacaa ataaaaaaac ggaggagtgc tttatgaaag cctatactta
    14041 tgttaaacca ggacttgctt cttttgttga tgtagacaaa ccagttattc gcaagccaac
    14101 agacgctatt gtgcgtattg taaaaaccac tatttgtgga acagacctcc atattatcaa
    14161 aggggatgtt cctacttgcc aaagtggtac cattcttggc cacgaaggga ttgggattgt
    14221 tgaagaagtt ggggaaggag tttccaactt caaaaaaggc gacaaggtct tgatttcttg
    14281 cgtctgtgcc tgtggtaaat gctactactg taaaaaagga atttatgccc actgtgaaga
    14341 cgaagggggc tggattttcg gtcacttgat tgatggtatg caggctgaat atctacgtgt
    14401 ccctcatgca gataatactc tttaccatac tccagaagac ttgtcagatg aagctttggt
    14461 tatgctgtca gacattctgc ctactggata tgaaattggt gtcttaaaag ggaaagtaga
    14521 acctggttgc agcgtagcca ttattggttc aggtccagtt ggattggctg ctcttttaac
    14581 agcccaattc tattcaccag ctaaattgat tatggtagac ctagacgata accgcttgga
    14641 aactgcccta tcattcggtg cgactcataa ggttaattct tcagacccta aaaaagccat
    14701 taaagaaatt tatgatttga cagatggtcg tggtgtggat gtcgctatcg aagctgtagg
    14761 gattcctgaa acatttgatt tctgtcaaaa gattatcggt gtagacggaa cggttgccaa
    14821 ctgtggtgtg catggtaaac cagttgaatt cgatttagat aaactttgga ttcgcaacat
    14881 caatgtaaca actggtttgg tatctacaaa tacgactcca caattgttga aagcacttga
    14941 aagtcataag attgaaccgg aaaaattggt aactcactat ttcaaactca gtgaaattga
    15001 aaaagcctac gaagtcttca gtaaggcagc agaccaccat gccattaagg tcattatcga
    15061 aaacgatatc tcagaagcct aagtagtaaa aatatttttg tacataagta aatagaaatt
    15121 cagtcatcca tcagatggtt ggatttttta tcaaaaaatt aagaaatgag catatttctt
    15181 tccttgtctg gcggaattgg ttataatata cggtacaaag gaatgaatga atatgtatcg
    15241 tgttatagaa atgtacggag attttgaacc gtggtggttc ttagaaggtt gggaagaaga
    15301 tattgtagca agtagaaaat ttgaccagta ttatgatgct ctcaaatact acaaaacttg
    15361 ctggtttaga ttggaacaag aatcgcctct ttataaaagt agaagcgact tgatgaccat
    15421 tttttgggac ccggaagacc aacgctggtg tgatgaatgt gatgagtatt tacaacaata
    15481 ccattctttg gctcttttgc aggatgagca ggttatccca gacgaaaaac tacgctcagg
    15541 ctatgaaaaa caaaccagtc aggaaaggaa tcgttcttgc cgtatgaaat taaaatagag
    15601 aaaagtaact ttttggagtt gcttttttta tttttctaac gctttgcgaa tagtataggt
    15661 gaggaggtaa gtatggttca agaaattgca caagaaatca ttcgttcggc tcggaaaaaa
    15721 ggggcgcaag acatttattt tgtccccaag ttagatgcct atgagcttca tatgagggta
    15781 ggagacgagc gctgtaaaat tggtagctat gattttgaaa agtttgcagc cgttatcagt
    15841 cactttaagt ttgtggcggg tataaatgtg ggagaaaaaa gacgtagtca actgggttcc
    15901 tgtgattatg cctatgacca gaagatggcg tctctacgtt tatctactgt aggcgattat
    15961 cgggggcatg agagtttggt tatccgtttg ttgcacgatg aggagcagga cttgcatttt
    16021 tggtttcagg atattgaaga attaggcaag cagtacaggc aacggggact ctatcttttt
    16081 gctggtccgg ttgggagtgg taagacgacc ttgatgcatg aattgtccaa gtcactcttt
    16141 aaaggacagc aagttatgtc catcgaagat cctgtcgaaa tcaagcagga cgacatgctt
    16201 cagttgcagt tgaacgaagc aatcggccta acctatgaaa atctaatcaa actttccttg
    16261 cgtcatcgac cagatctctt gattatcgga gaaattcgtg acagcgagac ggcgcgtgca
    16321 gtggtcagag ctagtttgac aggtgcgaca gtcttttcaa ccattcacgc caagagtatc
    16381 cgaggtgttt atgagcgtct gctggagttg ggtgtgagtg aagaagaatt ggcagttgtt
    16441 ctgcaaggag tctgctacca gagattaatc gggggaggag gaatcgttga ctttgcaaac
    16501 agagattatc aagaacacca agcagccaag tggaatgagc aaattgacca gcttcttaaa
    16561 gatggacata tcacaagtct tcaggctgag acggaaaaaa ttagctacag ctaagcaaaa
    16621 aaatatcatc accctattta acaatctctt ttctagcggt tttcatctgg tggagactat
    16681 ctccttttta gataggagtt ccttgttgga caagcagtgt gtgacccaga tgcgtacagg
    16741 cttgtctcag gggaaatcat tctcagaaat gatggaaagt ttgggatgtt caagtaccat
    16801 tgtcactcag ttatctctag ctgaagttca tggcaatctt cacctgagtt tgggaaagat
    16861 agaagaatat ctagacaatc tggccaaggt caagaaaaag ttaattgaag tagcgaccta
    16921 tcctttgatt ttgctgggtt ttcttctctt aattatgctg gggctacgga attacctgct
    16981 cccacaactg gatagtagca atattgccac ccgaattatc ggtaatctgc cacaaatttt
    17041 tctaggcatg gtagggcttg tttccgtgct tgccctttta gcactaactt tttataaaag
    17101 aagttctaag atgagtgtct tttctatctt agcacgcctt ccctttattg gaatctttgt
    17161 gcagacctac ttgacagcct attatgcacg tgaatggggg aatatgattt cacagggaat
    17221 ggagctgacg cagatttttc aaatgatgca ggaacaaggt tcccagctct ttaaagaaat
    17281 cggtcaagat ctggctcaaa ccctgaaaaa tggccgtgaa ttttctcaga cgataggaac
    17341 ctatcctttc tttaggaagg aattgagtct catcatagag tatggggaag ttaagtccaa
    17401 gctgggtagt gagttggaaa tctatgctga aaaaacttgg gaagcctttt ttacccgagt
    17461 caaccgcacc atgaatttgg tgcagccact ggtttttatc tttgtggcac tgattatcgt
    17521 tttactttat gcggcaatgc tcatgcccat gtatcaaaat atggaggtaa atttttaaaa
    17581 tgaaaaaaat gatgacattc ttgaaaaaag ctaaggttaa agcttttaca ttggtggaga
    17641 tgttagtagt cttgctgatt atcagcgtgc ttttcttgct ctttgtacct aatctgacca
    17701 agcaaaaaga agcagtcaat gacaaaggaa aagcagctgt tgttaaggtg gtggaaagcc
    17761 aggcagaact ttatagcttg gataaaaatg aagatgctac tctgagcaag ttacaaggag
    17821 atgggcgcat cacggaagaa caggttaaag cttataatga atactatact aaaaatggag
    17881 gggcaaatcg taaagtcaat gattaaggcc tttaccatgc tggaaagtct cttggttttg
    17941 ggacttgtga gtatccttgc cttgggctta tccagctctg tccagtccac ttttgcagca
    18001 gtagaggagc agattttctt tatggagttt gaagaactct atcgggaaac ccaaaaacgc
    18061 agtgtagcca gtcagcaaaa gactagtcta aatttagatg ggcagacgct tagcaatggc
    18121 agtcaaaagt tgacagttcc taaaggaatt caggcaccat caggccaaag tattacattt
    18181 gaccgagctg ggggcaattc gtccctggct aaggttgaat ttcagaccag taaaggagcg
    18241 attcgctatc aattatatct aggaaatgga aaaattaaac gcattaagga aacaaaaaat
    18301 tagggcagtg attttactgg aagcagtagt cgctctagct atctttgcca gcattgcgac
    18361 cctccttttg ggacaaattc aaaaaaatag gcaagaggaa gcaaaaatct tgcaaaagga
    18421 agaagtcttg agggtagcta agatggccct gcagacgggg caaaatcagg taagcatcaa
    18481 cggagttgag attcaggtat tttctagtga aaaaggattg gaggtctacc atggttcaga
    18541 acagttgttg gcaatcaaag agccataagg tcaaggcttt taccttgtta gaatccctgc
    18601 ttgccctcat tgtcatcagt gggggattac tcctttttca aactatgagt cagctcctca
    18661 tttcagaagt tcgctaccag caacaaagcg agcaaaagga gtggctcttg tttgtggacc
    18721 aacttgaggc agaattagac cgttcgcagt tcgaaaaagt agaaggcaat cgcctataca
    18781 tgaagcaaga tggcaaggac atcgccatcg gtaagtcaaa gtcagacgat tttcgtaaaa
    18841 ccgatgctag tggacggggg tatcagccta tggtttatgg cctcaaatca gctcagatta
    18901 cagaggacaa tcaactggtt cattttcgtt tccagtttca aaaaggctta gaaagggagt
    18961 tcatctatcg tgtggaaaaa gaagaaagtt aaggcaggtg ttctcctcta cgcagtcacc
    19021 atagcagcaa tctttagtct tttgttgcaa ttttatttga accgacaaat cgcccactat
    19081 caagactatg ctttaaataa agaaaaattg gttgcttttg ccatggctaa acgaaccaag
    19141 gataaagccg agcaagaaag tggggaacag gtttttaatc taggtcaggt aagctatcaa
    19201 aacaagaaaa caagcttggt gacgactgtt cgtacgtcta agagccaata tgagtttctg
    19261 tttccttcag tcaaaatcaa agaagagaaa agagataaaa aggaagaggt agcgaccgat
    19321 tcaagcgaaa aagcggagaa aaaaaatcag aagagaagcc tgaaaagaaa gagaattcct
    19381 agtcaattca actataatgc gttgaatcca gaatagtcca ctgtagtttc tagaaaattg
    19441 ctggaaatgg atgttaagct ccaattcatt tgtttatatc ttatttcagt ttactatact
    19501 ttgtgctaaa ctaaagatat gaaacatgat tttaaccaca aagcagaaac tttcgactcc
    19561 cctaaaaata tcttcctcgc aaacttggta tgtcaagcag tcgagaaaca gattgatatt
    19621 ttatcagaca aagtaatttt agatttcggt ggtggcacgg gtctattagc cttgccccta
    19681 gccaagcagg ctaagtcagt tactcttgta gacatttctg agaaaatgtt ggagcaagct
    19741 cgtttgaaag tggagcagca agcaatcaag aatatccagt ttttggagca agatttaccg
    19801 aaaaatccct tggagaaaga gtttgattgc cttgctgtta gtcgggttct tcatcatatg
    19861 cctgatttgg atgcggctct ctcactgttt catcaacatt tgaaggaaga tgggaaactc
    19921 atcattgctg attttaccaa gacagaagct aatcatcatg gatttgattt agctgaactg
    19981 gaaaacaagc taattgagca tggtttttca tctgtgcata gtcagattct ctatagcgct
    20041 gaagacctgt ttcaaggaaa tcactcagaa ttctttttaa tagtagccca aaaatcactc
    20101 gcctagtcag ggagtgattt ttctataagg atggaaaaaa gaagggaaat ttgataagat
    20161 aggaatatgg attttgaaaa aattgaacaa gcttatacct atttactaga gaatgtccaa
    20221 gttatccaaa gtgatttggc gaccaacttt tatgacgcct tggtggagca aaacagcatc
    20281 tatctggatg gtgaaactga gctaaaccag gtcaaagaca acaatcaggc ccttaagcgt
    20341 ttagcactac gcaaagaaga atggctcaag acctaccagt ttctcttgat gaaggctggg
    20401 caaacagaac ccttgcaggc caatcaccag tttacaccag atgccattgc tttgcttttg
    20461 gtgtttattg tggaagagtt gtttaaagag gaggaaatta ctatcctcga aatgggttct
    20521 gggatgggaa ttctaggcgc tactttcttg acctcgcttg ataaaaaggt ggattacttg
    20581 ggaatggaaa tggatgattt gctgattgat ctggcagcta gcatggcaga tgtaattggt
    20641 ttgcaggctg gctttgtcca aggagatgcc gttcgtccac aaatgctcaa agaaagcgat
    20701 gtggtcatca gtgacttgcc tgttggctat tatcctgatg atgccgttgc gtcgcgccat
    20761 caagttgctt ctagccaaga acatacttac gcccatcact tgctcatgga acaagggctt
    20821 aagtacctca agtcagacgg atacgctatt tttctagctc cgagtaattt gttgaccagt
    20881 cctcaaagtg atttgttaaa agaatggctg aaagaagagg cgagtctggt tgctatgatt
    20941 agtctgcctg aaaatctctt tgctaatgcc aaacaatcta agactatttt tatcttacag
    21001 aagaaaaatg aaatagcagt agagcctttt gtttatccac ttgctagctt gcaagatgca
    21061 agtgttttaa tgaaatttaa agaaaatttt caaaaatgga ctcaaggtac tgaaatataa
    21121 aatagatttt gttataatag ttgaaaacgc ttaaaaaggg gtatcatgtt atgacaaaaa
    21181 caattgcaat caatgcagga agttcaagtt tgaaatggca attatactta atgccagaag
    21241 aaaaagtgtt agccaaaggt ttgattgaac gtatcggttt gaaagattca atttcaactg
    21301 taaaatttga cggccgttct gagcaacaaa ttttggatat tgaaaatcat acacaagccg
    21361 ttaaaatttt attggatgac ttgattcgtt tcgatattat caaggcttat gacgagatta
    21421 caggtgttgg acatcgtgtt gttgctggtg gagaatattt caaagaatca acagttgttg
    21481 agggagatgt tttagaaaaa gttgaagagt tgagtttgtt ggctcctcta cacaacccgg
    21541 ccaatgcagc aggtgttcgt gccttcaagg aattgttgcc agacattacc agtgtagttg
    21601 tttttgatac ttccttccac acaagtatgc cagagaaagc ttatcgctac cctctaccaa
    21661 caaaatatta cacagaaaac aaggttcgta aatacggtgc tcatggtaca agtcaccagt
    21721 ttgtagcagg agaagctgca aaactcttgg gacgtccatt agaagacttg aagttaatta
    21781 cctgtcatat tggtaacgga ggctcaatta cagctgtgaa agccggcaaa tctgtagaca
    21841 cttctatggg gttcactcct cttggtggta ttatgatggg aacgcgtaca ggggatattg
    21901 atccagctat cattccttat ttaatgcaat atacagagga ttttaacaca ccagaagata
    21961 tcagtcgtgt tcttaaccgt gaatcaggtc ttttgggagt ttctgctaat tctagcgata
    22021 tgcgcgatat agaagcagct gtagcagaag ggaatcacga ggctagcttg gcttatgaaa
    22081 tgtatgttga ccgtatccaa aaacatatcg gtcagtacct tgcagtgcta aatggagcag
    22141 atgccattgt tttcacagca ggtgtcggtg aaaatgcaga gaatttccgt cgtgatgtaa
    22201 tctcagggat ttcgtggttt ggttgtgatg ttgatgatga aaagaatgtc tttggcgtta
    22261 caggagacat ctcaacagag gcagctaaaa tccgtgtctt ggttattcca acagatgaag
    22321 aattagtcat tgcccgtgac gttgaacgct tgaaaaaata agtgaaacta aaaaaatatt
    22381 caatacaagg agttgggaaa gttatttttc cagcttcttt ttctgatgaa attgtccaaa
    22441 accttgctat gattggcttt tttgaaaaat atggtataat agtagtaatt taatagatgg
    22501 agttgagttt tgaagaaaaa ctttcgtgta aaaagagaga aagattttaa ggcgattttc
    22561 aaggagggga caagttttgc taatcgcaaa tttgtggtct accaattaga aaaccagaaa
    22621 aaccattttc gagtaggtct atcagttagc aaaaaactgg ggaatgccgt cactagaaat
    22681 caaattaagc gacggattcg gcatattatc cagaatgcaa aagggagtct ggtagaagat
    22741 gtcgactttg ttgtcattgc tcgaaaagga gtcgaaacct tgggatacgc agagatggag
    22801 aaaaatctac tccatgtatt aaaattatca aagatttacc gggaaggaaa tgggagtgaa
    22861 aaagaaacta aagttaacta gtttgctagg actgtctctg ttaatcatga cagcctgtgc
    22921 gactaatggg gtaactagcg atattacagc cgaatcggct gatttttgga gtaaattggt
    22981 ttacttcttt gcggaaatca ttcgcttttt atcgtttgat attagtatcg gagtggggat
    23041 tattctcttt acggtcttga ttcgtacagt cctcttgcca gtctttcagg tgcaaatggt
    23101 ggcttctagg aaaatgcagg aagctcagcc acgcattaag gcgcttcgag aacaatatcc
    23161 aggtcgagat atggaaagca gaaccaaact agagcaggaa atgcgtaaag tatttaaaga
    23221 aatgggtgtc agacagtcag actctctttg gccgattttg attcagatgc cggttatttt
    23281 ggccctgttc caagccctat caagagttga ctttttaaag acaggtcatt tcttatggat
    23341 taaccttggt agtgtggata caacccttgt tcttccgatt ttagcagcag tattcacctt
    23401 tttaagtact tggttgtcca acaaagcttt gtctgagcga aatggcgcta cgactgcgat
    23461 gatgtatggg attccagtct tgatttttat ctttgcagtt tatgcgccag gtggagtcgc
    23521 cctatactgg acagtgtcta atgcttatca agtcttgcaa acctatttct tgaataatcc
    23581 attcaagatt atcgcagagc gcgaggccgt agtacaggca caaaaagatt tggaaaatag
    23641 aaaaagaaaa gccaagaaaa aggctcagaa aacgaaataa ataaggagga atctggtaat
    23701 ggtagtattt acaggttcaa ctgttgaaga agcaatccag aaaggattga aagaattaga
    23761 tattccaaga atgaaggctc atatcaaagt catttctagg gagaaaaaag gctttcttgg
    23821 tctatttggt aaaaaaccag cccaagtgga tattgaagcg attagtgaaa cgactgttgt
    23881 caaagcaaat caacaggtag taaaaggcgt tccgaaaaaa atcaatgatt tgaacgagcc
    23941 tgtgaagacg gttagtgaag aaaccgttga ccttggtcat gtggttaatg ctattaaaaa
    24001 aatagaggaa gaaggtcaag gtatttctga tgaagtcaag gctgaaatct taaaacatga
    24061 aagacatgcc agcactatct tagaagaaac tggtcacatt gagattttaa atgaacttca
    24121 aatcgaggaa gcgatgaggg aagaagcagg cgctgatgac cttgaaactg agcaagatca
    24181 aactgaaaat caagacttga aagagatggg cttgaaggtc gagcaaagtt atgatattgc
    24241 ccaggtggct acggatgtga ctgcctatgt tcaagcgatt gtggatgaca tggatgttga
    24301 ggctacactt tcaaatgatt ataaccgtcg tagcatcaat ctacaaattg acaccaacga
    24361 accaggtcgt attatcggct accatggtaa agtcttgaag gccttgcaac tgttggctca
    24421 aaattatctt tacaaccgct attccaaaac cttctacgtt acaatcaatg tcaatgatta
    24481 tgtcgaacac cgtgcagaag tcttgcagac ctatgcgcaa aaattggcga atcgtgtttt
    24541 ggaagaaggt cgcagtcata aaacagatcc aatgtcaaat agcgaacgca agattatcca
    24601 tcgtattatt tcacgtatgg atggcgtgac tagttactct gaaggtgatg agccaaatcg
    24661 ctatgttgtt gtagatacag aataagtaaa atcagggttg acctgatttt ttgctagtta
    24721 gaggaggtta aactgatgtt gaataagata agagactatc tagacttttc tggtttgcag
    24781 taccgtagtc ctgataaagc gggagcagag cgagagaaga tgctggcgtt ccgccacaaa
    24841 ggacaagagg cccgaaaggc ttttacagaa ctggccaaag cctttcaagc aagccatcca
    24901 gaatggcaac tccaacagac tagtcagtgg atgaatcagg cccagcgttt gagaccacat
    24961 ttttgggttt atctacagag agacggacaa gtgacagaac ctatgatggc cttacgtttg
    25021 tatgggacat ctactgactt tggaatttct ttggaagtca gtttcatcga acgtaagaag
    25081 gatgagcaaa cactgggcaa gcaggccaaa gttttagaca ttccaaccgt taaagggatt
    25141 tattatctaa cctactctaa tggtcaaagt caacggtggg aggcgaatga agaaaagcgt
    25201 cgtactttac gcgagaaggt gagaagtcaa gaagttcgaa aagttttagt gaaggtagat
    25261 gttcctatga cagaaaattc gtctgaagaa gaaatcgtag aaggcttatt gaagtcttat
    25321 tctaaaattc ttccctatta tctagctacg agaaaataag ataatttgta aaacatcata
    25381 aatcatacag tccaagagtg aacagtccgc tgtgtaattc ttggtctttt tgtttgcgct
    25441 ttcgcattat ataataaact tacaaaaaca attcaaaagg agaacaatta tggaagtcgt
    25501 ttcaagtgtt ctaaattggt tttctagcaa tattttgcag aatcccgcat ttttcgtagg
    25561 tttattggtg ttgataggat atgcactttt gaaaaaacct gcccatgacg ttttttcagg
    25621 gtttgttaaa gcaacagtag ggtatatgtt gcttaacgtg ggtgctggtg gtttggttac
    25681 aacctttcgt ccaatcttag cagctcttaa ctacaaattc caaattggtg cagcggttat
    25741 cgacccttat tttggacttg ctgcagcaaa caacaaaatt gtagcagagt ttccagattt
    25801 tgttggaact gcaactacag ctctattgat tggttttgga ataaatatct tgctcgtagc
    25861 tcttcgaaag attacgaagg taagaaccct ctttattact ggtcacatca tggtacaaca
    25921 agctgcaaca gtatctctta tggttctatt cttagtacca caattgcgca atgcttacgg
    25981 tacagcagcg attggtatca tctgtggact ttactgggca gttagttcaa atatgactgt
    26041 tgaggcaact caacgcttga ctggtggtgg cggatttgcg attggtcacc aacagcaatt
    26101 tgcaatctgg tttgtagata aagtagcagg acgctttggt aagaaagaag aaagtttaga
    26161 caatcttaaa ttacctaagt tcctctcaat cttccacgat acagttgttg catctgctac
    26221 cttgatgctc gtattcttcg gagccattct tttaatcttg ggtccagaca ttatgtctaa
    26281 taaagaagtc atcacttcag gaactctatt caatcctgct aaacaagatt tctttatgta
    26341 cattatccaa acagccttta ccttctcagt ttacttgttc gttttgatgc aaggtgtccg
    26401 aatgttcgta tctgagttga caaacgcctt ccaaggtatt tcaaacaaat tgttgccagg
    26461 ttcattccca gcggttgacg ttgcagcttc ttatggattt ggttctccaa atgctgtctt
    26521 gtcaggattt acctttggtt tgattggtca attgattaca attgtcttgc tcatcgtctt
    26581 taaaaatccg attcttatta ttacaggatt tgtaccagtg ttctttgaca atgcagccat
    26641 tgcggtctac gctgataaac gcggcggatg gaaagcggct gttatccttt cctttatatc
    26701 aggtgtcctt caagttgctc taggagctct ttgtgtggcc cttctcgatt tggcagctta
    26761 tggtggctac catggaaata tcgactttga attcccatgg cttggatttg gatatatctt
    26821 caaatacctt ggtattgttg gttatgtact tgtgtgtctc ttcttgcttg ttattcctca
    26881 acttcaattt gccaaagcaa aagataaaga gaaatattac aacggtgaag ttcaagaaga
    26941 agcttagtat ctagaaaagg agaaataaaa tggttaaagt attagcagcg tgcggaaatg
    27001 gaatgggttc atcaatggtt atcaagatga aggttgaaaa tgctctccgt aagcttaatc
    27061 aaagagattt tacagtcaat tcatgcagtg tcggtgaagc taaaggttta gcagtaggat
    27121 atgacatcgt aatcgcttct cttcatttga ttcaagaatt ggaagggcga actaatggga
    27181 agttaattgg gcttgataac ttgatggatg ataaagaaat caccgaaaaa ctcagtcaag
    27241 cactacagta aaaggttgga gggggctgga cagaaactga gagttatcgt ttctgtcctt
    27301 ctccctctta aataaaggag gcagatatga atttaaaaca agctttaatt gacaatgact
    27361 cgatccgact aggtttagag gctaacaatt ggaaagaagc agtcaaggta gcagtagatc
    27421 ccttaattga aagtggggca attttgccag agtattacga tgctatcatt gaatcgactg
    27481 aagagtatgg gccttactat atcttgatgc caggtatggc tatgccccac gctagacctg
    27541 aagctggtgt gcaaagtgat gccttttcat tgattacctt acaaaatcct gttgtatttt
    27601 cagatgggaa agaggtatct gttttgttgg cactagcagc aacaagctca aaaattcaca
    27661 caagtgtagc cattccacaa attattgccc tgtttgaatt agaagattct attgcacgtt
    27721 tacaggcttg ccagactaaa gaagatgtct tggctatgat tgaagaatct aaggatagcc
    27781 cttatctcga aggattggat ttggaaagtt agaaagagga ataaagaaat gacaaaaaga
    27841 atacctaatt tacaagttgc attagaccat tcagacttgc aaggagcgat taaagcagct
    27901 gtttctgttg gtcaggaagt agatattatc gaagctggaa ctgtttgctt gcttcaagtt
    27961 ggaagtgaac tggctgaagt cttgcgtagc cttttcccag ataagattat tgtggcagac
    28021 acaaaatgtg ctgatgctgg tggaacagtt gctaaaaata atgcggttcg tggagcagac
    28081 tggatgactt gtatctgttg tgcaaccatc cctactatgg aagcagctct aaaggctatc
    28141 aagactgaac gaggagaacg aggcgaaatc cagatcgagc tttatggcga ttggactttt
    28201 gaacaagctc agctttggct agatgcaggt atctcacaag ctatttatca ccaatctcgt
    28261 gatgctcttc ttgctggtga aacttggggt gaaaaagacc ttaataaggt taaaaaactc
    28321 attgacatgg gcttccgtgt atctgtaaca ggtggtctag atgtagatac tctcaaactc
    28381 tttgaaggtg ttgatgtctt tacctttatc gcaggtcgtg gaattacaga ggctgcggat
    28441 ccagcaggag cagcgcgtgc cttcaaggat gaaatcaaac gaatttgggg gtaaatcatg
    28501 gtacgtccaa ttggaattta tgaaaaggca accccaacac actttacttg gctagaacgt
    28561 ttaaattttg ccaaggagtt aggctttgat tttgtcgaga tgtctattga cgaacgtgac
    28621 gagcgtttag caagacttga ctggagtaag gaagaacgct tggaagttgt caaagcaatc
    28681 tatgaaactg gtgttcgtat tccttctatc tgtttttcag gccatcgtcg ctacccattg
    28741 ggttcaaaag atccagttct agaggaaaaa tctctagaac tcatgaaaaa atgtatcgaa
    28801 ttagctcaag acttgggagt tcgtacgatt caattagctg gttacgatgt ttactatgag
    28861 gaaaagtcac cccagacacg ccaacgtttt atcaaaaatt tgagaaaagc ctgtgactgg
    28921 gctgaagaag ctcaggtggt acttgctatt gaaattatgg atgatccttt catcagtagc
    28981 atcgaaaaat atttggctat agaaaaagag attgactctc ccttcctctt tgtatatcca
    29041 gatattggta atgtgtctgc atggcataat gatatctata gtgagtttta tcttggtcat
    29101 catgccatcg cagctctcca tctcaaggat acttatgcag tgacagaaag ttcaaggggc
    29161 cagttccgag atgtaccttt tgggcaaggt tgtgtcaaat gggaagaagc tttcgatatt
    29221 ttaaaggaaa ccaattataa tggacctttc ctaatcgaaa tgtggtctga aaattgtgaa
    29281 acagtagaag aaacacgcgc agccattcaa gaggcgcaag cttttctcta tccactcatt
    29341 aagaaagcag gtttgatgta agatgaatca agtaatcaat gctatgcgta aacgagtctg
    29401 tgatgccaat caatcattgc caaaacatgg acttgtcaaa tttacctggg ggaatgtatc
    29461 tgaagtcaat cgcgaactcg gtgtcattgt tatcaaacca tcaggcgtgg attatgacga
    29521 attgacacct gaaaacatgg tagtgactga tctagatggt aagatcctag aaggggattt
    29581 aagaccatct tccgacctcc caactcatgt gcaattatat agggcttggt cagaaattgg
    29641 tagtgtggtt cacacccatt cgacagaagc tgttggttgg gctcaggcag gtcgtgatat
    29701 tcctttctac ggaacaaccc atgcagatta tttctacggt tcaatccctt gcgcccgtag
    29761 tttgaccaag gacgaagtag aagtggccta tgaaaaagat actggcctgg ttatcgtaga
    29821 agaatttgaa catcgcggac ttaacccggt tgaagtacca ggaattgttg tacgcaatca
    29881 cggtccattc acctggggca aaaatccaga gaatgctgtt tatcactctg tcgtactaga
    29941 ggaagtatca aagatgaatc gctttacaga acaaatcaat ccaagagttg gacctgctcc
    30001 ccagtacata ctagaaaaac actaccaacg taaacatgga ccaaatgctt attatggtca
    30061 aaagtaagaa cgatgaagga ggagaaaaag ataaatttag ctcctctttt tacatttgat
    30121 ttttattgag agtaaagttg gagttgaagt aattttgaaa gattttttag aaatagcgc
//
LOCUS       DAVMGZ010000010        27984 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000010 DAVMGZ010000000
VERSION     DAVMGZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.010"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            351..1514
                     /locus_tag="VYQ93_000381"
     CDS             351..1514
                     /locus_tag="VYQ93_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV3335127.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRSAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHCFIVMDVFEYF
                     KYAKRKNLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1511..2188
                     /locus_tag="VYQ93_000382"
     CDS             1511..2188
                     /locus_tag="VYQ93_000382"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV3335128.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2178..3032
                     /locus_tag="VYQ93_000383"
     CDS             2178..3032
                     /locus_tag="VYQ93_000383"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV3335129.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3051..4118
                     /locus_tag="VYQ93_000384"
     CDS             3051..4118
                     /locus_tag="VYQ93_000384"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV3335130.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSPEEMKDYLVK"
     gene            4128..5294
                     /gene="aroC"
                     /locus_tag="VYQ93_000385"
     CDS             4128..5294
                     /gene="aroC"
                     /locus_tag="VYQ93_000385"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV3335131.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5304..6407
                     /locus_tag="VYQ93_000386"
     CDS             5304..6407
                     /locus_tag="VYQ93_000386"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV3335132.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITKNTDYTVVIK"
     gene            6418..6756
                     /locus_tag="VYQ93_000387"
     CDS             6418..6756
                     /locus_tag="VYQ93_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV3335133.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6848..8131
                     /gene="aroA"
                     /locus_tag="VYQ93_000388"
     CDS             6848..8131
                     /gene="aroA"
                     /locus_tag="VYQ93_000388"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV3335134.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITVQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8124..8600
                     /locus_tag="VYQ93_000389"
     CDS             8124..8600
                     /locus_tag="VYQ93_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV3335135.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQRDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8597..9445
                     /gene="pheA"
                     /locus_tag="VYQ93_000390"
     CDS             8597..9445
                     /gene="pheA"
                     /locus_tag="VYQ93_000390"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV3335136.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWLLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9442..10716
                     /gene="psr"
                     /locus_tag="VYQ93_000391"
     CDS             9442..10716
                     /gene="psr"
                     /locus_tag="VYQ93_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV3335137.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFVYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10995..11840
                     /locus_tag="VYQ93_000392"
     CDS             10995..11840
                     /locus_tag="VYQ93_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV3335138.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKKT
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFFPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11830..12933
                     /locus_tag="VYQ93_000393"
     CDS             11830..12933
                     /locus_tag="VYQ93_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV3335139.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12935..13921
                     /locus_tag="VYQ93_000394"
     CDS             12935..13921
                     /locus_tag="VYQ93_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3335140.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDARVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATDLSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13937..15370
                     /locus_tag="VYQ93_000395"
     CDS             13937..15370
                     /locus_tag="VYQ93_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV3335141.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15399..16433
                     /locus_tag="VYQ93_000396"
     CDS             15399..16433
                     /locus_tag="VYQ93_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV3335142.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16633..17370
                     /gene="mecA"
                     /locus_tag="VYQ93_000397"
     CDS             16633..17370
                     /gene="mecA"
                     /locus_tag="VYQ93_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV3335143.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17521..18807
                     /locus_tag="VYQ93_000398"
     CDS             17521..18807
                     /locus_tag="VYQ93_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV3335144.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDVTIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18809..19678
                     /locus_tag="VYQ93_000399"
     CDS             18809..19678
                     /locus_tag="VYQ93_000399"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV3335145.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19748..19828
                     /locus_tag="VYQ93_000400"
                     /pseudo
     CDS             19748..19828
                     /locus_tag="VYQ93_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            19973..20911
                     /gene="msrB"
                     /locus_tag="VYQ93_000401"
     CDS             19973..20911
                     /gene="msrB"
                     /locus_tag="VYQ93_000401"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV3335146.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20969..22693
                     /locus_tag="VYQ93_000402"
     CDS             20969..22693
                     /locus_tag="VYQ93_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3335147.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGTRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLITSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSDRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            22695..24443
                     /locus_tag="VYQ93_000403"
     CDS             22695..24443
                     /locus_tag="VYQ93_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3335148.1"
                     /translation="MKRQTVNQTLKRLVVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKKSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24745..26004
                     /locus_tag="VYQ93_000404"
     CDS             24745..26004
                     /locus_tag="VYQ93_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV3335149.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26080..26215
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26254..26754
                     /locus_tag="VYQ93_000405"
     CDS             26254..26754
                     /locus_tag="VYQ93_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV3335150.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26830..27198
                     /gene="rplL"
                     /locus_tag="VYQ93_000406"
     CDS             26830..27198
                     /gene="rplL"
                     /locus_tag="VYQ93_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV3335151.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27385..27972
                     /locus_tag="VYQ93_000407"
     CDS             27385..27972
                     /locus_tag="VYQ93_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3335152.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQVLYGASLFS
                     FLWMYGQRKQVVKVNMKTRMKCLGIGLASLLIISLCFSLIHAQGSTNQTNLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALVHHPGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNFLVYSLLLF"
BASE COUNT         8242 a         5214 c         6302 g         8226 t
ORIGIN      
        1 aggcttttct ataaaatcaa attttccaga tatctactga agctgattta ttttttcatc
       61 tttcttctac tacctttgtc actaaatttg acagacacat attgctgata caacctctct
      121 acctactaat ctcacagtta acctgaggct agtttcatag tttgctattt gattttcgtt
      181 gataaaaaaa atgctcctcc aagacggaag agcatttttt attgtgattc ttacacattt
      241 acttcctagt tttgcaggaa gtctagtata ccatttttta aaagaagagt aaaaaagcaa
      301 gggtagtttc ttttttgaga attgtctaaa agtttgatat aatggtagtt atgaatagaa
      361 taagagtcag caaaagggtt gaaaagaagc ttgctaaggg gctagtttta ctagaagcca
      421 gtgatcttga gaatgtcaat cttaaggatc aggaagtaga ggtgcagggt caggaaggaa
      481 actttcttgg gactgcctac ctttctcagc aaaacaaggg cttgggctgg tttgtcagca
      541 aagacaaggt ggccttcaat caagctttct ttgaaacgtt gtttagaaaa gccaaagaaa
      601 agagaagcgc ctactatcaa gatgatttaa caactgcctt tcgtctcttt aatcaagagg
      661 gagatggctt tgggggtctg acagtggacc tttatggcga ctacgccgtc ttttcttggt
      721 ataactctta tgtttatcag attcgtcaga ctatatcaga agcctttaga caggttttcc
      781 ctgaggtttt aggggcttat gagaaaatcc gctttaaggg tttggactat gaatctgccc
      841 atgtttatgg tcaagaagca cctgactttt tcactgtttt agaaaatggt gtcctgtatc
      901 aagtctttat gaatgatggc ttgatgacag gaattttcct agaccagcat gaggttcgcg
      961 gtagtttagt tgacggcttg gctatgggta aatccttgct caatatgttt tcctacacag
     1021 cggctttttc agtagctgcg gccatgggag gagctagcca gacaacttct gttgatctag
     1081 ccaaacgttc acgagaattg tctcaagcgc attttcaggc aaatgggctc agcacagacg
     1141 agcattgttt tatagtcatg gatgtctttg agtatttcaa atatgccaaa cgtaaaaact
     1201 tgacctacga tgtgattgtc ctagatccgc ctagctttgc tcggaataaa aaacaaactt
     1261 tctctgtggc caaggattat cacaagttga tttcccagag tcttgagatt ttaaatccgg
     1321 gagggattat cattgccagt accaatgctg ccaatgtttc ccgtcagaaa tttacagaac
     1381 aaattgataa aggctttgca ggaagaagtt accagatttt aaacaaatat ggtcttccag
     1441 cagattttgc ctataataaa aaagatgaaa gtagtaatta cctcaaggtg attagtatga
     1501 aggttagtaa atgaaattaa tcgtttcagt aatgccaaga agtttagagg aggctcaggc
     1561 tctggatgcc acgaggtacc tggatgccga catcattgaa tggcgtgccg actatctgcc
     1621 taaagaagcg attttgcagg tagctccagc catttttgaa aaattcgcag gccgtgagtt
     1681 ggttttcacg ctacgaactc gctccgaagg tggagaaatc gacctttctc cagaagagta
     1741 tatccatcta atcaaggaag ttgcgcaact ctatcaacca gactatattg attttgagta
     1801 ctatagctac aaggatgttt ttgaggaaat gctggacttc ccaaatcttg ttttgagtta
     1861 ccataatttc caagaaacac ctgagaacat gatggaaatc ttgtcagagt tgacgatcct
     1921 aaatccaaaa cttgttaagg ttgcggtgat ggctcacacg gagcaggatg tcttagactt
     1981 gatgaactat acacgaggct ttaaaaccct caatcctgaa caggaatatg tgaccatttc
     2041 tatgggtaag gtgggcaagg tctctcgtat cactgcggat gtgactggtt cgagttggtc
     2101 ttttgccagt ctggatgagg ttagtgcccc tgggcagatt tctctagcta gcatgaaaaa
     2161 aatcagggag attttggatg aagcttgatg gctatacacg tttagctgca gttgttgcca
     2221 atcctattaa gcattctatt tctcccttca tccacaatag agcctttgag gcgacagcta
     2281 ccaacggtgc ttatgtggct tgggagattg aagcgagtga cttggtagaa acagtggcca
     2341 atattcgtcg ctaccagatg tttggcatca atctgtccat gccctataag gagcaggtga
     2401 ttccttattt ggatgagctg agcgatgaag cgcgcttgat tggtgcggtt aatacggttg
     2461 tcaatgagaa tggcaattta attggatata atacagatgg caagggattt tttaagtgct
     2521 tgccttcttt tacaatttca ggtaaaaaga tgaccctgct gggtgcaggt ggtgcggcta
     2581 aatcaatctt ggcacaggct attttggatg gcgtcagtca gatttcggtc tttgttcgtt
     2641 ccgtttctat ggaaaaaaca agaccttacc tagacaagtt acaggagcag acaggcttta
     2701 aagtggattt gtgtgcttta gaatatgttt ctgaactgca agcaaggatt gccgagtcgg
     2761 atttgctagt taatgccacc agtgtgggca tggatggcca atcctctcct gttcctgaaa
     2821 acatagtctt accagaaact cttttagttg cagatatcat ataccaaccc tttgaaacac
     2881 cgtttttgaa atgggctaga agtcagggca atccagcagt caatggtctg ggaatgttgc
     2941 tctatcaagc tgcagaagct tttcaactgt ggacaggcaa ggaaatgccg acagaagaga
     3001 tttggcagtc tttaacagaa aaataccaat aaagaaaggg agactctcct atgaaaatca
     3061 gaatcgatat tcctcatcat ccttatgata ttcagattga aaaaggttgt atggcccagg
     3121 ctggtcagtg gttgcgagaa ctctggcaac cgcaaaaggt agtcattgtg acagataacc
     3181 atgtagcctc tctctatgca gagaaggtca agctcagcct agaagatgct ggttttcagg
     3241 tagctgtttt tgatttctta gaaggtgaag aaagaaagaa tttaactact gttcagaaag
     3301 tctatgaatt tctagtcaag caaggtctga ctcgtagcga tggaatcgtt gctcttggtg
     3361 gtggcgttgt tggggacctg gctggctttg tagcctctac ctatatgcgg ggtattcact
     3421 ttgttcagat tccgactagt ttgacagctc aggttgattc ttctatcggt ggaaagacag
     3481 gcgttaacac accatttgct aaaaatatgg tggggacctt tgcccaacca gatggggttc
     3541 tgattgatcc acttgttctt gaaaccctcg gaaaaagaga gttgattgaa gggatgggtg
     3601 aagttatcaa gtatggcttg attgaggatc cagaactgtg ggctctcttg acgggactga
     3661 atggttctgt tgagagtatt ttggaacatg cagagacctt gattgaacat tcttgtcagg
     3721 tgaagcgcaa gatggtggtt gaagatgagt tggacaatgg tattcgtctt tacctcaatt
     3781 ttggccacac tattggccat gccatcgaag cgactgccgg ttatggcaag gtcatgcatg
     3841 gagaggctgt tgccatggga atggtacaga tttccaaggt tgctgaggaa aaaggcctca
     3901 tgccagctgg cattacccaa tctatcacag agatgtgtca gaaattcggc ttgcctgttg
     3961 actatgaaaa ttggaaagtt gacaagcttt atcaggctct tactcatgac aagaaagcgc
     4021 gtggtaacac cttgaaattg gtcttggtgc cagagcttgg ttcagcgacc attcacccag
     4081 tttctccgga agagatgaaa gactacttgg taaaataagg agagtgtatg agatatttaa
     4141 ctgcaggaga atcacacggc ccccgtctaa cagctattat tgagggaatt ccagctggac
     4201 ttccattgac agctgaggat atcaatgagg atcttagacg ccgtcagggt ggctacggtc
     4261 gtggtggtcg tatgaagatt gagagtgacc aggttgtctt tacttcgggc gttcgccacg
     4321 ggaagacgac aggggcgcct attactatgg atgtcatcaa taaggaccac cagaaatggc
     4381 tggacatcat gtctgcggag gacattgaag accgccttaa aagcaagcgg aaaattactc
     4441 atcctcgccc aggtcatgcc gatttggttg gggggattaa gtaccgtttt gatgatttgc
     4501 gaaattcttt ggagcgttca tcagctcgtg aaaccaccat gcgggtggca gttggtgcag
     4561 tagccaaacg tctcttggct gagctggata tggagattgc caaccatgtc gtggtctttg
     4621 gtggcaagga aatcgatgtt cctgaaaatc tgacagtcgc tgaaattaag caaagagctg
     4681 cccagtctga agtttctatt gtcaaccaag aacgagaaca ggaaatcaag gactatattg
     4741 accaaatcaa acgtgatggt gataccatcg gtggggttgt ggagacagtc gtcggaggcg
     4801 ttccagttgg tcttggttcc tatgtccaat gggatagaaa attggatgca agattggctc
     4861 aagctgttgt ctctatcaat gcctttaaag gggtggaatt tggtcttggc tttgaggctg
     4921 gttatcgtaa aggcagccaa gttatggatg aaattctctg gtctaaagaa gacggttata
     4981 ctcgccgtac caataatcta ggtggttttg aaggtggtat gactaatggg caacccatcg
     5041 ttgttcgtgg ggtcatgaaa cccattccta ctctttataa acctcttatg agtgtggata
     5101 tcgaaaccca cgaaccttac aaggcaaccg tggagagaag tgatccgact gctcttccag
     5161 ctgcaggaat ggtcatggaa gcagttgtag caacggttct ggcgcaagaa atcctcgaaa
     5221 aattctcatc agataatctt gaggaactaa aagaagcggt agccaaacac cgagactata
     5281 caaagaacta ttaaggagtt cctatggcaa aaacaatcta tatcgcaggt cttgggttga
     5341 ttggagcctc tatggcactt ggtatcaaac gcgatcatcc agattatgaa attttaggtt
     5401 ataatcgtag tcaagcttcg agagatatcg ccttgaaaga aggcatgatt gaccgtgcaa
     5461 cggatgattt tgctagtttt gctcctttgg cagatgtcat tatcctcagc ttgccaatca
     5521 aacaaactat tgctttcatt aaggagttgg ccaatttgga tttgcgagaa ggcgttatta
     5581 tttcagatgc tggttcgacc aagtcaacca ttgtggatgc ggcggagcag tatttggctg
     5641 gcaagtctgt tcgctttgtc ggggcccatc ccatggctgg tagtcacaag acaggggctg
     5701 cttcggcaga tgtcaatctt tttgaaaatg cctattatat ctttacacct tcaagcctga
     5761 caagtcagga cacgcttaag gaaatgaagg atctgctttc aggtcttcat gctcgtttta
     5821 tcgagattga tgccaaggag catgatcgtg tcacttctca gattagccat tttcctcata
     5881 ttttggcttc tagtctcatg gagcagactg cggtctatgc tcaagagcat gagatggcaa
     5941 ggcgctttgc ggcaggtggt tttcgagata tgacccgaat tgcggaaagc gagccaggaa
     6001 tgtggacctc cattctcttg tccaatagcg agaccattct ggatagaatt gaggatttca
     6061 aggaacgttt ggaagcgatt ggtcaggcca ttagtaaggg agatgaagag caaatttgga
     6121 acttttttaa ccaagcgcgt gaacaacgcc agaccatgga aatccataag cgtggtggtg
     6181 tggatagctc ttacgacctc tatgttgacg ttcctgatga agaagatgtc atcctgcgga
     6241 ttttggaact gctacgtggg acttccttgg ttaacatcca catcaacgag gaaaatcgtg
     6301 aggatattca cggaatccta caaatttcat ttaaaaatgc tcaagacttg gaaagagccg
     6361 agcatctcat aacaaaaaat accgactata cagtcgtcat taaataagga gaagaacatg
     6421 tcaaatattt acgatagtgc aaacgaactc agtcgcggtc tacgcggatt accagaatac
     6481 aaggctgtta aagcagctaa agatgcgatt gcagcagatg ctgaggcaag caaaatcttt
     6541 acagaatatc ttgcctttca agaggaaatt caaaaactag cacagacagg tcaaatgcca
     6601 gatgcgtcct tccaagctaa gatggaaggc tttggtaaac agattcaagg aaatagcctc
     6661 ttgtcagaat tctttaccaa gcaacaacaa ttggccattt acctttctga cattgaaaaa
     6721 attgttttcg aaccagtttc agaattgctg aaataagaaa agaactatcc taaagtcagg
     6781 aatttttaag gaatttctgg ctttttatga taaaataggt aaaagtattg caagtatgag
     6841 gtccagtatg aaactaaaaa caaacattcg ccatttacat ggtagtatcc gcgtcccagg
     6901 tgacaagtct atcagccacc gttccattat ctttggaagt ttggctgagg gtgagaccaa
     6961 ggtttatgat attctgcgag gtgaagacgt tctttcgacc atgcaggttt ttcgtgacct
     7021 tggtgttgaa attgaggata aagatggggt tattaccgtt caaggtgtag gcatggctgg
     7081 cttaaaagcg ccgcaaaatg cccttaatat gggaaattct ggcacctcga ttcgcctgat
     7141 ttcaggtgtc cttgctggtg cagatttcga agtagagatg tttggagatg atagtctttc
     7201 caaacgtcct atggaccgtg tgacccttcc actgaaaaaa atgggcgtca gcatctcagg
     7261 gcaaactgaa cgagacttgc ctccccttcg cttaaaaggg acgaaaaacc taagacctat
     7321 tcattatgag ttgccaattg cctctgccca agtcaagtca gccttgatgt ttgcagcctt
     7381 acaagctaag ggggagtcag ttattatcga aaaagagtac acccgtaatc atactgaaga
     7441 tatgttgcaa caatttggtg gtcatttaag tgtggatggt aagaaaatca cagtccaagg
     7501 gccacaaaaa ttgacaggac agaaggtggt cgtaccagga gatatttcca gtgcagcctt
     7561 ttggttagtc gcaggtttga ttgctccaaa ttctcgtcta gtgctgcaga atgtggggat
     7621 aaacgaaact cgcaccggta ttattgatgt cattcgtgcc atgggtggaa aattggaaat
     7681 aactgaaatc gatccagtcg ctaaatctgc aaccttgatt gttgagtctt ctgacttgaa
     7741 aggaacagag atttgtggcg ctttgattcc acgtttgatt gatgaattgc ctattattgc
     7801 cctacttgcg acccaagccc aaggtgtaac agttatcaag gatgctgagg agctcaaggt
     7861 caaggaaaca gaccgtattc aggttgtggc agacgcctta aatagtatgg gagcagatat
     7921 tactcctacg gcagatggga tgattatcaa aggaaaatca gctcttcacg gtgctagagt
     7981 caatacgttt ggtgaccacc gtatcggcat gatgacagct atcgcagccc tattggttgc
     8041 agatggagag gtggagcttg accgtgcaga agccatcaat accagctatc ctagtttctt
     8101 tgatgatttg gagagcttga ttcatggcta aggtgttatt agggtttatg ggggctggaa
     8161 aatcgactat tgcaagaggc ttggacccta attaccttga tatggatgct ctgattgaga
     8221 agcgcctagg tatgtccatt gcgaattttt tcgctgaaaa gggagaagca gcctttcgtc
     8281 aggtagaatc agaagtccta gctgatttat tacaaagaga ccaagtcgtg tcaactggag
     8341 gaggagtggt tatttctcag agaaatcgtg acttactcaa gactaataca gataacatct
     8401 acctgaaagc agattttgaa accctctacc tacgtatcgc agctgataag gacaatcagc
     8461 gaccgctttt tctaaataat agcaaggaag aactagtagc tatttttcaa gaaagacagg
     8521 cttggtatga ggaagtggct agtcgggttt tggatgtgac caagctaagc ccagaggaaa
     8581 ttatagagga actaagatga aaattgctta tctaggtccc aaaggatcat tttcacacca
     8641 cgttgtgcag acagcttttc ctcatgagga attgcaggct tttgccaaca ttacagatgt
     8701 catcaaggct tatgagcaag gattagtgga ctattctgtg gtgccagttg aaaattctat
     8761 tgagggtagt gttcatgaaa ccttggacta tctttttcat caggctcaca tccaagcagt
     8821 agcagaaatc gttcagccta ttcatcagca gttgatggtg gttccaggcc atactaagat
     8881 tgaaaagatt ttttcacatc cacaggcctt ggctcaagga aagaaattca tcgatgaaca
     8941 atatccagag gctcaaatcg aggtaacagc tagtacagct tatgcggccc gttttatttc
     9001 cgaacatcca gatcagccct ttgcagcaat tgcacctaga agttctgctg aagaatatgg
     9061 attggaactg attgccgagg atattcagga aatggaagcc aatttcacac gtttctggct
     9121 tctaggagct gaaaagccta gtattccctt gcaagcacaa actgaaaaga tgagtttggc
     9181 cttgacatta cctgacaacc ttccaggtgc actttataag gccctgtcga cctttgcttg
     9241 gcgaggaatt gacttgacaa aaattgaaag tcgtccactc aagacagcac tgggtgaata
     9301 ctttttcatt atcgatgtgg attataccga taaggacttg gtccactttg cccaaaaaga
     9361 attagaagcg attggaatcc agtataaaat tctgggtgcc tatcctattt atccaatatc
     9421 agaccatgga aaggagagaa gatgagtaaa gaaaatccct taagtcatca tgagcagttg
     9481 cgttatgatt atttgctaaa aaatattcac tatctcaatg agagagaaaa aaatgagttt
     9541 gtctatttgc aagaaaagct aactcttgct aggggaaata gtagctctag cttggaacaa
     9601 gaaagagaag agcaggttga cttaccaagc tatgcgaacc ggagtcgctc acaatccaaa
     9661 tcacaagcac tctctttccc tccaaaaaag aaaagacgga agctccgtct taaacgaatt
     9721 tttatggtga ttttttcact tttggtctgt gtggctttgg ccatggtatt catgtttttg
     9781 cgtggttacc aagatgctag cgcaaagaaa actgctgatg cccgggcagc tcaagtagaa
     9841 gtctttaatg gtcaggacac tagagatgga gttaatattt taatcatggg tactgatggt
     9901 cgaatcggcc agaacagtgt tgagacacga actgactcta ttatggtatt aaatgtcggg
     9961 ggctcagata agaaaatgaa gctagtcagt ttcatgcgtg ataatttggt ctatatagat
    10021 ggttatagtc aagtgattaa cggtagaaaa cagacagata acaagttaaa cgtagcctac
    10081 gagttaggag aacaagaagg gcaaaaaggg gcagaaatgg ttcgccaagt cttgaaagat
    10141 aattttgact tggacattaa gtactacgcc ttggttgatt tccaggcctt tgccacagcg
    10201 attgacacgc ttttccctga tggggtgacg attgatgctc aattttcaac attgaatggg
    10261 cgtcccctaa cagaagctac agtcggagat gatttatacg ctaccgagac tgagtctcca
    10321 acccaaacca ttaaagtcgg aaaacagcag atgaatggct cgaccctgct caattatgct
    10381 cgtttccgtg atgatgatga ggcggattac ggccgtacaa aaagacagca acaagtttta
    10441 acagcaattt tagagcaaat taaagatccc actaaacttt tcactggttc tgaagctctt
    10501 ggaaaggttt ttgctatgac ctcaaccaac gtaccctata ctttcctcct aacaaacggt
    10561 ttatctgttt tggatggagc aaaaaatggt attgaaaaat tgacgattcc agaactgggt
    10621 gactgggtag atgcctatga tgtttatgga ggcttgggct tgctggttga tcaaaacaaa
    10681 tatcaaacca agctcgctca aatgggcttg agataagata atactcaatg aaaatcaaag
    10741 tgcaaattag gaagctagcc gcaggctgct aaaaacacag ttttgaggtt gtagataaga
    10801 ctgacgaagt cagttaccta tacctacggc aaggtgaagc tgacgtggtt tgaagagatt
    10861 ttcgaagagt ataagacaga aaaatcaaag tttgatagct atttggatag cagtcaggct
    10921 ttgatttttt acagtctgca atagattttc accccttgtt tgtggtataa tgaaacgata
    10981 cgatagaaag aataatgaac aatatgactg atttaaaagc aattcaggct cgtagtctgg
    11041 agatggctga atattttgtg gccttttgta aagaacatga tttgctctgt tatctctgtg
    11101 gcggaggcgc tattggtacc cttcgaaaca agggatttat tccttgggac gacgacctag
    11161 acttttttat gcctcgtaaa gattatgaga aattagcaga attatggcct cgttatgcag
    11221 atgaacgtta tttcttgtca aagagtcaca aggattttgt tgatcgtaat ctttttatta
    11281 ccattcgtga caagaaaacc acctgtatca agccttatca gcaggatttg gatttgccac
    11341 atggtctggc cttggatgtt ttgcctttgg attattatcc gaaaaatcca gctgagcgga
    11401 aaaaacaggt tcgttgggcc ttgatttatt cactcttttg tgcgcaaact attccagaaa
    11461 agcatggtgc tctcatgaaa tggggaagtc gcattttact gggtttgact ccaaaatctc
    11521 tccgttatcg catctggaaa aaagctgaga aagaaatgac taagtatgat ttggctgatt
    11581 gtgatggcat tacagaatta tgctcaggtc ctggctacat gagaaacaag tacccaatca
    11641 catcttttga agacaatctt ttctttccat ttgaaggaac agagatgcct attccaatcg
    11701 gctatgatgt ctatctcaga actgcttttg gggattatat gacgcctcca ccagcagaca
    11761 agcaggtacc gcatcatgat gctgtcatcg ctgatatgga taagtcttat acagaataca
    11821 agggagaata tggtggctaa gaaaaaaatc ttatttttta tgtggtcttt ttctcttgga
    11881 ggtggtgcag agaagattct atcaaccatt gtttcaaatc tggatccaga aaagtatgat
    11941 attgatattc ttgaaatgga gcactttgac aagggatatg aatctgttcc aaagcatgta
    12001 cgcattttaa aatcccttca agattatcgc caaaccagat ggttacgagc ttttttgtgg
    12061 agaatgagaa tttattttcc aagactggct cgtcgtttgc ttgtaaaaga tgattatgat
    12121 gttgaagttt cttttaccat tatgaatcca ccactgttgt tctctaaaag aagagaagtc
    12181 aagaagatat cttggattca tggaagtatt gaagaacttc ttaaggatag ctctaaaaga
    12241 gaatcacata gaagccagtt ggatgctgcg aatacaattg tagggatttc aaaaaagacc
    12301 agcaattcta tcaaggaagt ttatccagat tatgcttcta aattacagac aatctacaat
    12361 ggatatgatt ttcagactat tctagaaaaa tctcaagaga agatcgatat cgagattgct
    12421 cctcaaagta tctgtactat cggacggatc gaggaaaata agggttctga ccgtgtagtg
    12481 gaagtgatac gattattaca ccaagaggga aaaaactatc atctctattt tatcggggct
    12541 ggtgatatgg aagaggaact gaaaaaacga gtcaaagagt atgggattga ggactatgta
    12601 catttccttg gttatcaaaa aaatccttat cagtatttat ctcagacgaa agttctcttg
    12661 tctatgtcta aacaagaagg ttttcctgga gtgtatgtgg aggccttgag tctgggactc
    12721 ccttttatct ctacggacgt tggaggggct gaggaattat cccaagaagg acgatttgga
    12781 caaatcattg agagcaatca agaggcagct caggcgatta ctaattacat gacttctgcc
    12841 tcaaactttg atgtcgatga ggctagccaa ttcattcaac aatttacaat tacaaaacaa
    12901 atcgaacaag tagaaaaact attagaggag tagcatggaa actgcattaa ttagtgtgat
    12961 tgtgccagtc tataatgtgg cgcagtacct agaaaaatcg atagcttcca ttcagaagca
    13021 gacctatcaa aatctggaaa ttattcttgt tgatgatggt gcaacagatg aaagtggtcg
    13081 cttgtgtgat tcaatcgctg aacaagatgc cagggtgtca gtgcttcata aaaagaacga
    13141 aggattgtcg caagcacgaa atgatgggat gaagcaggct cacggggatt atctgatttt
    13201 tattgactca gatgattata tccatccaga aatgattcag agcttatatg agcaattagt
    13261 tcaagaagat gcggatgttt cgagctgtgg tgtcatgaat gtctatgcta atgatgaaag
    13321 cccacagtca gccaatcagg atgactattt tgtctgtgat tctcaaacat ttctaaagga
    13381 atacctcata ggtgaaaaaa tacctgggac gatttgcaat aagctaatca agagacagat
    13441 tgcaactgac ctatcctttc ctaaggggtt gatttacgaa gatgcctatt accattttga
    13501 tttaatcaag ttggccaaga agtatgtggt taatactaaa ccctattatt actatttcca
    13561 tagaggggat agtattacga ccaaacccta tgcagagaag gatttagcct atattgatat
    13621 ctaccaaaag ttttataatg aagttgtgaa aaactatcct gacttgaaag aggtcgcttt
    13681 tttcagattg gcctatgccc acttctttat tctggataag atgttgctag atgatcagta
    13741 taaacagttt gaagcctatt ctcagattca tcgtttttta aaaggccatg cctttgctat
    13801 ttctaggaat ccaattttcc gtaaggggag aagaattagt gctttggctc tattcataaa
    13861 tatttcctta tatcgattct tattactgaa aaatattgaa aaatctaaaa aattacatta
    13921 gaacgggggt gaccaagtga ttgatgggaa acgattatta tttagtttga ccatagtcag
    13981 ttatgccttg acgctagtaa gtggaattgt gtatctgttt aataataata atgttagctt
    14041 actttctact ttattgttct tgttggttag tagcttaatt gcttgttgga acgatatcaa
    14101 gtattactta atccatttta ttttctattt aaccattttt gtatttctgg tatcaagacc
    14161 gaccattgat tattttaggg atggtgcttt ggatacctat catccaatag cctatcgttt
    14221 tgcctttata gttgtcatgg tttcgattct gggcttgacc acaggaggca ttctggctcg
    14281 ctacttcata gctaggaaga aaataaaagt agcaaatata ggaaattctc taaaagaggt
    14341 ttatatcaag cggttacgct ttgtatcact aggagttttt cttctaacct atcctttcta
    14401 tttcattcga ttatttgaac ggctcttgta tcgtttgcag acttcctact atgcctacta
    14461 tgcaaatttt gaaagtaaac tgccttattt tacctacatt ttgtctacct ttacggtcta
    14521 tgcaatgtgt atgtatctgg caaccaagcc aaagaaattg caggccacag cagtgcttgt
    14581 ctcctttatt gcagctaata ctattcattt ggcaattggg acacgaaatc cctttatttt
    14641 aagtatttta tttgcttttg tttattactt tatgcgggag caaactgaaa aaggaaaatg
    14701 gattgggttt aaagaaaagt tagcgatttt tgtaggttct cctattctca tgttagcgat
    14761 gggagtactc aattatgtac gggataatgt ccaagtttcc catacaggtt tctgggatat
    14821 cttacttgac tttatctata aacaagggac tagttttggt gttctggctc gaggttttct
    14881 atttaacagt agcctccctt accgagattt ccgtaatttt acttttggtc ctgttcttga
    14941 ttattttgca agggggagtt tgggagctat tttcggagga aaagcctttg aacatacaac
    15001 caatagtgtg gaactagcta ttgatagtaa tagttatgcc cacaatctat cctatcttgt
    15061 cttgaacaag gaatacctga aagggcatgg tatcggaagt agttatatca tggagttgta
    15121 taccgactat ggtatgattg gagtctttct gcttagtttc ttactcggcg tattatttat
    15181 agccatgctg caagtagcct atcgctcaag gacaatccta tttgctttat ccctactcat
    15241 cttgaataat ctattcttta tgccaagaag cagcttttca gaaagtttct tcaatttatt
    15301 tacaatgcaa ttctggggaa ttgttcttgt gattatattt gtagcaaaaa tgcttacaaa
    15361 ggaaaactag tatttactaa acaaaggaga aaaaaatcat gtttgaacat tattcagtag
    15421 ctgatttgtt tgcaaatctt tataaaaaac gaaaagcaaa tattttagct ctcatcgctt
    15481 tatttgctct cattgctgta ccatttacaa ttaaagcagt taggaataaa aacactgtca
    15541 aagatacaac aagttattca acttatctta tctataaaat cactcctcca gaagagtcgg
    15601 ccaaaacgat tttgaatcat caaattggtg gttatagtga tttttatggg aaattgattg
    15661 atggtaattt gaatggagct tatcttttca atgatgtaga acccagtgag ttgaaaaaaa
    15721 ttgccagtga attagacacg acagaaacaa ccttgaaaaa ttcaacgaat gactattggt
    15781 ggaaaaaatt gaccgtctac tatatgattg acgatgcagg ggttggtgtg aaaattttga
    15841 catcaagtaa agatgccaat gacttgttag agaaaaaaat tgatgggttg attgagaaat
    15901 ttaaacatgc ttatgcaaat gtgaaaattg aaaaactgga aaccatcaac tctaaagaat
    15961 tgaacgcaaa tggtgaaaca gcgcttggct taaatgtgaa aaatctgatt cttcgtttag
    16021 ttgttattgg agtggtttgt gtgattttgg ttgtgatggg aaatgtgtta gtttatctct
    16081 ttaatccaac aatcaataga gtaggtgatt tttctcagta tcaaattgat tttgtaacag
    16141 agattacaac aattgctaac ctagcagatg ttttgtcata caaaaatact ggacaggaat
    16201 tgaccatcgt tagctcaaat aaagctatcc tagataaatt gaaacagagt caagaagctt
    16261 taaaaggaat gcattttgta gatttacagg atgtatcatc tcttttggaa agagatacag
    16321 tccttcttgt tgaagagtac ggagtgactc gttataagaa atttgagcaa agtcttcaaa
    16381 ttctcagaaa cttaaatcgt tctatcctcg gtgtagcaac ctttaaatta taagtattct
    16441 gatgtataag gtcttcaaaa tcggctctta gccgattttg aaggcttttt ctatctgtct
    16501 gcagaaattt tcttttggag tctcctatag aaagacccta gggaaaatct atccgtatag
    16561 aggagccttc ttttgataaa atcgtaaaaa tctagtataa tagatagaac tgaaagtatg
    16621 aggttactag caatgaaaat gaaacaaatt agtgatacaa ctttaaaaat cacgatgtct
    16681 ttagaggatt tgatggatcg tggaatggag attgctgact ttctcgttcc tcaagaaaaa
    16741 acagaagagt tcttttatgc tatcttggat gagctagaga tgcctgatag ctttctggat
    16801 acaggcatgt tgagcttccg tgtgactcca aaacctgata aggtagatgt ctttgtgacc
    16861 aagtcaaaga ttgaccaaaa tctagatttt gaagacttat cggatttgcc agatatggaa
    16921 gaattggctc aaatgtctcc agatgaattt atcaaaaccc tggaaaaaag catcgcagac
    16981 aaaaccaagg atgatatcga agccattcaa tctcttgagc aagttgaagc caaggaagaa
    17041 gagcaagagc aagctgaaca agaagctgag agtaagaaag aaccttacat ctactacatc
    17101 ctttcttttg ctaagttggc tgacttggta gcttttgcca agacagtgac ttttgaaatg
    17161 gaaacttctg aactctacaa aatgaacgag cgctattatt tgaccatttt agtggatatt
    17221 gaaaatcatc caagcccata tccagcttgg ctgttggccc gtatgcgcga gtttgcagac
    17281 gatagtgata tcagtcgctc agtcttacaa gagtatggtc aagtcttgat gagtcacgat
    17341 gcagtgctca atctgcaaaa aatcggctaa tcctttctgg aaagctattt tcagatttta
    17401 agaagagcga atcgtctgat tcgttttttc tttttatact gaaatagtga tttactataa
    17461 taggaatttt cacaaaattc tgttataatg gctatattag aaaatttcga ggagataaag
    17521 atgacagtta aaattgcttt actgggattt ggtaccgttg caagtggtgt gcctttcctc
    17581 ctaaaggaaa atggaggaaa aatcaatcaa tcagcacatt cagatatcaa agttgctaag
    17641 gtattggtca aggatgaaga tgaaaaaaat cgcttgcttg cagcagggaa tgactttaac
    17701 tttgtaacca atgtggatga tattttatca gaccaggatg ttactatcgt agtggaattg
    17761 atggggcgta ttgagcctgc taaaaccttt atcactcgtg ccttggaagc tggaaaacac
    17821 gttgttactg ctaacaagga ccttttagct gtccatggcg cagaattgct agaaatcgct
    17881 caagctaaca aggtagcact ttactacgaa gcagcagttg ctggtgggat tccaattctt
    17941 cgtactttag caaattcctt ggcttctgat aaaattacgc gcgtgcttgg agtagtcaac
    18001 ggaacttcca acttcatggt gaccaagatg gtggaagaag gctggtctta cgatgatgct
    18061 cttgcggaag cacaacgtct aggatttgca gaaagcgatc cgacgaatga cgtagatggg
    18121 attgatgcag cctacaagat ggttattttg agccaatttg cctttggcat gaagattgcc
    18181 tttgatgatg tagcccacaa gggaatccgc aatatcacac cagaagacgt agctgtagct
    18241 caagagcttg gttacgtagt gaaattggtt ggttctattg aggaaacttc ttcaggtatt
    18301 gctgcagaag tgactccaac cttcctacct aaagcgcacc cacttgctag tgtgaatggc
    18361 gtaatgaacg ctgtctttgt agaatctatc ggtattggtg agtctatgta ctacggacca
    18421 ggtgcgggtc aaaaaccaac tgcaacaagt gttgtagctg atattgtccg tatcgttcgt
    18481 cgtttgaatg atggtaccat tggcaaagac ttcaacgaat atagccgtga cttggtcttg
    18541 gcaaatcctg aagatgtcaa agcaaactac tatttctcaa tcttggctct agactcaaaa
    18601 ggtcaggtct tgaagttggc tgaaatcttc aatgctcaag atatttcctt taagcaaatc
    18661 cttcaagatg gcaaagaggg tgacaaggcg cgtgtcgtta tcatcacaca caagattaat
    18721 aaagcccagc ttgaaaatgt ctcagctgaa ttgaagaagg tttcagaatt cgacctcttg
    18781 aataccttca aggtgctagg agaataagat gaagattatt gtacctgcaa ccagtgccaa
    18841 tatcgggcca ggttttgact cggtcggtgt agctgtaacc aagtatcttc aaattgaggt
    18901 ctgcgaagaa cgagatgagt ggctgattga acaccagatt ggcaaatgga ttccacatga
    18961 tgagcgtaat ctcttgctca aaatcgcttt acaaattgta ccagacttgc aaccaagacg
    19021 cttgaaaatg accagtgatg tccctttggc gcgcggtttg ggttcttcca gctcggttat
    19081 tgttgctggg attgaactag ccaaccaact gggtcaactc aacttatcag accatgaaaa
    19141 attgcagtta gcgaccaaga ttgaagggca tcctgacaat gtggctccag ccatttatgg
    19201 taatctcgtt attgcaagtt ctgttgaagg gcaagtctct gctatcgtag cagactttcc
    19261 agagtgtgat tttctagctt acattccaaa ctatgaatta cgtactcgcg acagccgtag
    19321 tgtcttgcct aaaaaattgt cttataagga agctgttgct gcaagttcta tcgccaatgt
    19381 agcggttgct gccttgttgg caggagacat ggtgaccgct gggcaagcaa tcgagggaga
    19441 cctcttccat gagcgttatc gtcaggactt ggtaagagaa tttgcgatga ttaagcaagt
    19501 gaccaaagaa aatggggcct atgcaaccta cctttctggt gctgggccga cagttatggt
    19561 tctggcttct catgacaaga tgccaacaat taaggcagaa ttggaaaagc aacctttcaa
    19621 aggaaaactg catgacttga gagttgatac ccaaggtgtc cgtgtagaag caaaataaag
    19681 aatagaagat aggatgggga aactcttgac cagagggttt catatccttt ttgtgaaaag
    19741 aagtttatac tcaatgaaaa tcaaagagca aactaggaag ctagccgcag gttgctcaaa
    19801 gcactgcttt gaggttgtag ataagactga cgaagtcagc tcaaaacagt gttttgaggt
    19861 tgcagataga actgacgaag tcagtaacat atatacggta aggtgacgct gacgtggttt
    19921 gaagagattt tcgaagagta ttagttaaaa acgtgataaa ggagaaataa aaatggcaga
    19981 aatttatcta gcaggtggtt gtttttgggg cctagaggaa tatttttcac gcatttctgg
    20041 agtgctagaa accagtgttg gctacgctaa tggtcaagtc gaaacgacca attaccagtt
    20101 gctcaaggaa acagaccatg cagaaacggt ccaagtgatt tacgatgaga aggaagtgtc
    20161 actcagagag attttacttt attatttccg agttatcgat cctctatcta tcaatcaaca
    20221 agggaatgac cgtggtcgcc aatatcgaac tgggatttat tatcaggatg aagcagattt
    20281 gccagctatc tacacagtgg tgcaggagca ggaacgcatg ctgggtcgaa agattgcagt
    20341 agaagtggag caattacgcc actacattct ggctgaagac taccaccaag actatctcag
    20401 gaagaatcct tcaggttact gtcatatcga tgtgaccgat gctgataagc cattgattga
    20461 tgcagcaaac tatgaaaagc ctagtcaaga ggtgttgaag gccagtctat ctgaagagtc
    20521 ttatcgtgtc acacaagaag ctgctacaga ggctccattt accaatgcct atgaccaaac
    20581 ctttgaagag gggatttatg tagatattac gacaggtgag ccactctttt ttgccaagga
    20641 taagtttgct tcaggttgtg gttggccaag ttttagccgt ccgatttcca aagagttgat
    20701 tcattattac aaggatctga gccatggaat ggagcgaatt gaagttcgtt ctcgttcagg
    20761 cagtgctcac ttgggtcatg ttttcacaga tggaccgcgg gagttaggcg gcctccgtta
    20821 ctgtatcaat tctgcttctt tacgctttgt ggccaaggat gagatggaaa aagcaggata
    20881 tggctatcta ttgccttact taaacaaata aaacagagag tggggcttcc cactttcttc
    20941 atttctagaa tatgaataga agggatttat gaaacaccta ttatcttact tcaaacccta
    21001 catcaaggaa tcaattttag cccccttgtt caagctgtta gaagctgttt ttgagctctt
    21061 ggttcccatg gtgattgctg ggattgttga ccaatcttta cctcagggag atcaaggtca
    21121 tctctggatg cagattggtc tgctccttat ctttgcagta attggcgttt tagtggcctt
    21181 gatagctcaa ttttactcag caaaggcagc agtaggtttt gctaaggaat tgacaaacga
    21241 tctttatcgt catattcttt ccttgcccaa ggacagcaga gaccgtctga caacttctag
    21301 tttggtcact cgcttgactt cggataccta ccagattcag actggtatca atcaattcct
    21361 gcgtctcttt ttacgagcgc ccattatcgt ttttggtgcc atttttatgg cttatcgaat
    21421 ctcagctgag ttgactttct ggttcttagt catggttgcc attttgacca ttgtcattgt
    21481 agggttatct cgattggtca atcctctcta cagtagtctc agaaagaaaa cggaccaact
    21541 ggttcaggaa acgcgccagc aattgcaagg gacgcgggtt attcgtgctt ttggtcaaga
    21601 aaaacgagag ttacagattt ttcaaaccct taaccaagtt tatgctagat tacaagaaaa
    21661 gacaggtttc tggtctagtt tattaacacc tctgacctat ctgattgtca atggaactct
    21721 tctcgttatt atctggcaag gctatatttc aattcaagga ggagtgctca gtcaaggtgc
    21781 tctcattgct cttatcaatt acctcttaca gattttggtg gaattggtca agcttgccat
    21841 gctgatcact tccctcaacc agtcctacat ctcagccaag agaatcgagg aggtctttgc
    21901 ggaatctccc gaaaacatcc attcagaatt agaacaaaag caagttacca gtgatcgggt
    21961 tttacaagtc caagaattga cctttaccta tcctgatgcg gcccagcctt ctctgagaga
    22021 catttccttt gatatgactc aaggacaaat ccttggtatc attgggggga ctggttctgg
    22081 taaatcaagc ttggtgcaac tcttacttgg actttatcca gtagacaagg ggaacattga
    22141 cctttatcaa aatggacgta gtcctcttaa tttggagcag tggcggtctt ggattgccta
    22201 tgtacctcaa aaggtcgaac tctttaaagg aaccattcgt tccaacttga ctctaggttt
    22261 caatcaagaa gtatctgacc aggaactctg gcaggccttg gagattgcgc aagctaagga
    22321 ttttgtcagt gaaaaggaag gactcttgga tgctctagtt gaggcagggg ggcgaaattt
    22381 ctcaggtgga caaaaacaaa gattgtctat cgcccgagca gtcttgcgcc aagctccgtt
    22441 tctcatccta gatgatgcaa cctcggcact ggataccatt acagagtcca agctcttgaa
    22501 agctattaga gaaaattttc caaacacgag cttaattttg atctctcaac gaacctcaac
    22561 tttacagatg gcggaccaga ttctcctctt ggaaaaaggt gagttgctag ctgttggcaa
    22621 gcacgatgac ttgatgaaat ccagccaagt ctattgtgaa atcaatgcat cccaacatgg
    22681 aaaggaggac tagaatgaaa cgacaaactg taaaccagac gctcaaacgt ttagtcgtag
    22741 atttagcaag ccatcctttc ctccttttcc tagcctttct aggaactatt gctcaagttg
    22801 gcttatcaat ttacctacct attctgattg ggcaggtcat tgaccaagtc ctagtggctg
    22861 gttcatcacc agttttttgg cagatttttc tccagatgct cttggtggta ataggaaata
    22921 ctctggtaca atgggccaat cctctcctct ataatcgtct aatcttctct tataccagag
    22981 atttacggga gcgaatcatc cataagctcc atcgtttacc gattgccttt gtagataggc
    23041 aaggtagtgg agagatggtt agtcgtgtaa ccacggacat cgaacagttg gcagctggct
    23101 tgaccatgat ttttaaccaa tttttcattg gtgttttgat gattttggtc agtattctag
    23161 ccatgctcca aattcatctc ctcatgactc tcttagtctt gctgttgacg ccactgtcca
    23221 tggtgatttc acgctttatt gccaagaaat cctatcatct cttccagaag caaacagaga
    23281 cgaggggaat tcagactcag ttgattgaag aatcgcttag tcagcagact ataatccagt
    23341 ccttcaatgc tcaaacagaa tttatccaaa gattgcgtga ggctcatgac aactactcag
    23401 gctattctca gtcagccatc ttttattctt caacggtcaa tccttcgact cgctttgtaa
    23461 atgcactcat ttatgccctt ttagctggag taggagctta tcgtatcatg atgggttcag
    23521 ccttgaccgt cggtcgttta gtgacttttt tgaactatgt tcagcaatac accaagccct
    23581 ttaacgatat ttcttcagtg ctagctgagt tgcaaagtgc tctggcttgc gtagagcgta
    23641 tctatggagt cttagatagc cctgaagtgg ctgaaacagg taaggaagtc ttgaccagtg
    23701 accaagttaa gggagctatt tcctttaaac atgtctcttt tggctaccat cctgaaaaaa
    23761 ttttgattaa ggacttgtct atcgatattc cagctggtag taagatagcc atcgttggtc
    23821 cgacaggtgc tggaaaatca actcttatca atctccttat gcgtttttat cccattagct
    23881 cgggagatat cttgctggat gggcaatcca tttatgatta tacacgagca tcattgagac
    23941 agcagtttgg catggtgctt caagaaacct ggctcacaca agggaccatt catgataata
    24001 ttgcctttgg caatcctgaa gccagtcgag agcaagtaat tgctgctgcc aaagcagcta
    24061 atgcagactt tttcatccaa cagttgccac agggatacga taccaagttg gaaaatgctg
    24121 gagaatctct ctctgtcggc caagctcagc tcttgaccat agcccgagtc tttctggcta
    24181 ttccaaagat tcttatctta gacgaggcaa cttcttccat tgatacacgg acagaagtgc
    24241 tggtacagga tgcctttgca aaactcatga agggccgcac aagtttcatc attgctcacc
    24301 gtttgtcaac cattcaggat gcggatttaa ttcttgtctt agtagatggt gatattgttg
    24361 aatatggtaa ccatcaagaa ctcatggata gaaagggtaa gtattaccaa atgcaaaaag
    24421 ctgcggcttt tagttctgaa taagccattc tcttttgaaa gtttatggac gaaaaaagtt
    24481 gccttcgagt gacttttttg ttacaatagc tagaaaaatt gtttactgta atactcaatg
    24541 aaaatcaaag agcaaactag gaagctagcc gtaggttgct caaaacactg ttttgaggtt
    24601 gcagatagaa ctgacgaagt cagctcaaaa aactgttttg aggttgcaga tagaactgac
    24661 gaagtcagta accacaccta cggcaaagtg aatttgaagt ggtttgaaga gagtacaact
    24721 tgtcttttag aaaaggagcc tataatgaaa gtctttcagc atgtaaatat cgtgacttgt
    24781 gatcaagatt tccatgttta tcttgatgga atcttagcag tcaaggattc tcaaatcgtc
    24841 tatgtcggtc aagataagcc agcgttttta gagcaagctg agcagattat agactatcag
    24901 ggagcttgga ttatgcctgg tttggtcaat tgtcacaccc attctgcaat gacaggcctg
    24961 agagggatcc gagatgacag caatctccat gaatggctca atgactatat ctggccagca
    25021 gaatctgagt ttactcccga catgactacc aatgcggtca aagaagccct aacagagatg
    25081 ctccagtcag gaacaacaac ctttaacgat atgtataatc ccaatggtgt ggatatccag
    25141 caaatttatc aggtggtgaa aacttccaag atgcgttgtt atttttctcc gactctcttt
    25201 tcttcagaga cagaaacaac tgctgagact ataagcagaa ctcgatccat catagacgaa
    25261 atcttaaaat ataaaaatcc aaatttcaag gttatggtag cacctcattc tccgtatagc
    25321 tgcagtagag acttgctgga agcgagtttg gaaatggcaa aagagctaaa tattccgctc
    25381 catgtccatg tggcggagac caaggaggag tcaggaatta tcctcaaacg atacggcaaa
    25441 cgcccccttg cttttcttga agaactgggt tatttagatc atccgtccgt ctttgctcac
    25501 ggggtcgaat taaacgagag agaaattgaa cgcttggcat cttctcaagt ggctatcgcc
    25561 cacaatccta tcagtaacct caaactggca tcaggaattg ctccaattat ccagctccaa
    25621 aaagcgggag tagcagtcgg aattgcgact gactcggttg cttccaataa caatctagat
    25681 atgtttgagg aaggaaggac tgcagctctt cttcagaaga tgaaaagtgg ggatgccagc
    25741 cagtttccaa tcgaaacagc tctcaaggta ctgacaatcg aaggggctaa ggtccttgga
    25801 atggaaaatc agataggaag tctggaagtc ggcaagcaag cagattttct ggtcattcaa
    25861 ccacaaggga aaattcatct ccaacctcag gaaaatatgc tgtctcacct ggtttatgca
    25921 gttaaatcta gtgatgtaga tgatgtttat atcgccggag aacaggttgt taagcaaggt
    25981 caagtcctga cagtagaact ttaaaagaaa aattacgaaa aattttaaaa aaagtttgca
    26041 aaaatcttgc attctttttt tgactatgct atacttatat acggtttaaa aaaactgcct
    26101 aagacagtag gggagctcga ctcataaata tcctaccgag gacaaaacgt atcatgtaaa
    26161 aagaagcgta ttgtactttc gtgtctaggt ttgggcgcgt ttttcttttt gaaaaattcc
    26221 ccaagcaaaa taattacgga ggtgaacaca ctaatgagtg aagcaattat tgctaaaaaa
    26281 gcggaactag ttgacgtagt agctgaaaaa atgaaagctg ctgcatctat cgtcgttgta
    26341 gacgctcgtg gtttgacagt tgagcaagat acagttcttc gtcgtgagct tcgtggaagc
    26401 gaagttgagt ataaagttat taaaaactca atcttgcgtc gtgcagctga aaaagctggt
    26461 cttgaagatc ttgcatctgt atttgttgga ccatctgcag tagcattttc taatgaagat
    26521 gttatcgcac cagcgaaaat cttgaacgac ttttctaaaa acgctgaagc acttgaaatt
    26581 aaaggtggtg caatcgaagg cgctgtcgca tctaaagaag agattcttgc acttgcaact
    26641 cttccaaacc gcgaaggact tctttctatg ctcctttctg tacttcaagc gccagtgcgc
    26701 aacgttgctc ttgcagtcaa agcggttgca gaaagcaaag aagacgcggc ttaatcttaa
    26761 gctacatagc gtagcctagc tacgaaaaaa actattataa aatttaaaac ttatttggag
    26821 gaaataacaa tggcattgaa cattgaaaac attattgctg aaattaaaga agcttcaatc
    26881 cttgaattga acgaccttgt aaaagctatc gaagaagaat ttggtgtaac tgcagctgct
    26941 cctgtagctg ttgctgcagc tgatgctgct gatgctggtg ctgctaaaga ttcattcgac
    27001 gttgaattga catctgcagg cgacaaaaaa gttggtgtta tcaaagttgt acgtgaaatc
    27061 actggtcttg gtcttaaaga agctaaagaa cttgttgacg gtgcaccagc acttgttaaa
    27121 gaaggcgttg caactgcaga agctgaagaa atcaaagcta aattggaaga agctggagct
    27181 tcagttactc ttaaataata gagtatcgca atttgattgc ggaatcccta tttaccagtc
    27241 tttgagagcg ataagcgatt gatagaaact gataaattga tttggtttcc tgccaaaaat
    27301 tctttggcag gatttttgtt ttaataaact ttcgggggta gacactagat tgtctacccc
    27361 tttttttgtg tttcggaggt aactatgaaa agaataattc cagtttatat attccaacaa
    27421 gtaaatgtcc tattggtatc tctatactta ttgaaatttc tttgtatcgg tgagttaact
    27481 atactacagg ttctctatgg tgcgtctctt ttttcttttt tatggatgta tggtcaacga
    27541 aaacaagtgg tcaaggtcaa tatgaaaact aggatgaaat gccttggtat tggactagct
    27601 agtctactga ttataagtct atgttttagc ctgattcatg ctcaaggaag cacgaatcaa
    27661 acaaatttaa ttggccttca acatcaagtt ccttggtttt catttttatt gttcttaatc
    27721 aatgcgagta tggttgaaga atttctgtat cgtgaaattt tatggaactt ggttagaaaa
    27781 ttagatattc gagttgcttt gacaagtgtt ttatttgcct tagtacatca tccaggaacc
    27841 attctagctt ggtgtctgta cgtttcactt ggaatgtttt taggtatggt gcgctataaa
    27901 tctgacttat ggggaagtat ggggttacat ttggtgtgga actttttagt ttatagtttg
    27961 ctgctttttt aaaataggtg acct
//
LOCUS       DAVMGZ010000011        27774 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000011 DAVMGZ010000000
VERSION     DAVMGZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27774
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.011"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            442..1620
                     /locus_tag="VYQ93_000408"
     CDS             442..1620
                     /locus_tag="VYQ93_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3335153.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRSALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            1934..2065
                     /locus_tag="VYQ93_000409"
     CDS             1934..2065
                     /locus_tag="VYQ93_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV3335154.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            2086..3636
                     /gene="dltA"
                     /locus_tag="VYQ93_000410"
     CDS             2086..3636
                     /gene="dltA"
                     /locus_tag="VYQ93_000410"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV3335155.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWISTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDSVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3633..4877
                     /gene="dltB"
                     /locus_tag="VYQ93_000411"
     CDS             3633..4877
                     /gene="dltB"
                     /locus_tag="VYQ93_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV3335156.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            4891..5130
                     /gene="dltC"
                     /locus_tag="VYQ93_000412"
     CDS             4891..5130
                     /gene="dltC"
                     /locus_tag="VYQ93_000412"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV3335157.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            5123..6391
                     /gene="dltD"
                     /locus_tag="VYQ93_000413"
     CDS             5123..6391
                     /gene="dltD"
                     /locus_tag="VYQ93_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV3335158.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6546..6986
                     /gene="adcR"
                     /locus_tag="VYQ93_000414"
     CDS             6546..6986
                     /gene="adcR"
                     /locus_tag="VYQ93_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV3335159.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            6986..7690
                     /locus_tag="VYQ93_000415"
     CDS             6986..7690
                     /locus_tag="VYQ93_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV3335160.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7683..8489
                     /locus_tag="VYQ93_000416"
     CDS             7683..8489
                     /locus_tag="VYQ93_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV3335161.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8499..10004
                     /gene="adcA"
                     /locus_tag="VYQ93_000417"
     CDS             8499..10004
                     /gene="adcA"
                     /locus_tag="VYQ93_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV3335162.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(11166..11939)
                     /locus_tag="VYQ93_000418"
     CDS             complement(11166..11939)
                     /locus_tag="VYQ93_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV3335163.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            12115..13518
                     /locus_tag="VYQ93_000419"
     CDS             12115..13518
                     /locus_tag="VYQ93_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV3335164.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSTLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEDSLFISSGTWSLVGVELSSPILSTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETGEWTDGQLFKIVYESLAETYKETIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            13633..14271
                     /locus_tag="VYQ93_000420"
     CDS             13633..14271
                     /locus_tag="VYQ93_000420"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV3335165.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            14283..14723
                     /locus_tag="VYQ93_000421"
     CDS             14283..14723
                     /locus_tag="VYQ93_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV3335166.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            14716..15147
                     /locus_tag="VYQ93_000422"
     CDS             14716..15147
                     /locus_tag="VYQ93_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3335167.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            15138..15608
                     /locus_tag="VYQ93_000423"
     CDS             15138..15608
                     /locus_tag="VYQ93_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3335168.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            15633..16406
                     /locus_tag="VYQ93_000424"
     CDS             15633..16406
                     /locus_tag="VYQ93_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV3335169.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            16403..17203
                     /locus_tag="VYQ93_000425"
     CDS             16403..17203
                     /locus_tag="VYQ93_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV3335170.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            17262..19556
                     /locus_tag="VYQ93_000426"
     CDS             17262..19556
                     /locus_tag="VYQ93_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV3335171.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            19566..22682
                     /locus_tag="VYQ93_000427"
     CDS             19566..22682
                     /locus_tag="VYQ93_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV3335172.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQILSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            22739..24505
                     /locus_tag="VYQ93_000428"
     CDS             22739..24505
                     /locus_tag="VYQ93_000428"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV3335173.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            24651..25802
                     /locus_tag="VYQ93_000429"
     CDS             24651..25802
                     /locus_tag="VYQ93_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV3335174.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFRDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGRPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            25947..26831
                     /locus_tag="VYQ93_000430"
     CDS             25947..26831
                     /locus_tag="VYQ93_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV3335175.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            27229..27480
                     /locus_tag="VYQ93_000431"
     CDS             27229..27480
                     /locus_tag="VYQ93_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV3335176.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFAGMGDQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            27468..27758
                     /locus_tag="VYQ93_000432"
                     /pseudo
     CDS             27468..27758
                     /locus_tag="VYQ93_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8728 a         4547 c         5889 g         8610 t
ORIGIN      
        1 acggcacgta cggtggtgtg agaggggcta gagattatcc cctactcgat taactcccct
       61 gaaatttatt ttaattatgc aaatttcacg tatttttgat gctgagacga cgatcctggg
      121 aacttttcag atattttttt gactatctaa atctatcatt agaaaagctt agagcgccaa
      181 aggatttgag cgtttttctg atttttaaga ctttttccca gtctcttttt cgattgaaga
      241 tgtaattatt ctactaacta actaacttct tagtactagc caacaacgat aatcataatt
      301 cctcctaaaa ttaggaataa taaaggcaat agtttttgtt ttttcatgta aaaaacctca
      361 cttttgtttt ctgctatttt atgctaaaat attaaaaatc aaatttaatt ccaaagtttg
      421 taactaaagg gggagcgcta catgtctaat tcatttgtca aattgttagt ctctcaatta
      481 tttgcaaatt tagcagatat tttctttaga gtaacaatca ttgctaacat atacattatt
      541 tcaaaatcag taattgccac atcactagtt cctatcttaa taggaatatc ctcttttgtt
      601 gcgagtcttt tagttccgtt ggttactaaa aggtcagcgc taaatagggt tttatcttta
      661 tctcaatttg gaaagactat attattggcg atactggtag gaatgtttac cgtaatgcaa
      721 tccgtagcgc ctttggtgac ctatctattt gttgttgcaa tttccatact agatggtttt
      781 gcagcacccg tttcctatgc tattgtgcca cgctatgcga ccgatttggg taaggctaat
      841 tcagccttat caatgactgg tgaagctgtt caattgatag gttggggatt aggtggactc
      901 ttgtttgcaa caattggtct gttacctacc acgtttatca ttttagtctt gtatatcatt
      961 tctagctttc tgatgttatt tcttcctaac gctgaagtgg aggtgttaga gtcagaaact
     1021 aatcttgaaa ttttgctcaa aggttggaag ttagttgcta gaaatcctag attaagactt
     1081 tttgtatcag caaatttatt ggaaattttt tcaaatacga tttgggtttc ttccattata
     1141 cttgtttttg taacggagtt attaaataaa acggaaagtt actggggata ttctaataca
     1201 gcatactcta ttggtattat aattagtggc ttaattgctt ttaggctatc tgaaaagttc
     1261 cttgctgcta aatgggaaag tattcttttt cctcttgtag ctatggcaat agtgacactg
     1321 actattctat attttccaaa cgcacagatg tttttattat tttcagcttt agttggtatg
     1381 ttatcgcaac taaaagaagt tcctgaaagt gtatttcttc aggaaacagt agaggaaaat
     1441 catttagtta atgtctattc cgttcttgaa gtgatttcta cattagcatt ttcagtattt
     1501 gttttgctaa tgagctatat tactgagagt tttggtatta gcatcagttt ttggctatca
     1561 gccatttgtc tgatgataga agctatttta atttatatca gacgagatta ttttaaatga
     1621 gagagtggtt aaaaagtctt taaaaatcag aaaagcgcat agtatcaggt gttgaaaaaa
     1681 cttgatattg tgtgttttat tatgggaaga tttacttcat tgtctcctga aatggagttt
     1741 tgaagcagga tcactctatt tgaaaatata catagaaaaa ctgtataatc cttaaaataa
     1801 gctgaaatct attggaaaca tacggtaaac aattgaaaag atactatata aagtggtaca
     1861 atggtagaaa aataagctag taagaatcac tcttatttta gcgaaaaatt acagaaatga
     1921 atggtttttc ttgatgaaac agagaaaaga attgtacctc tttcttggtc ggacagcctt
     1981 gtattttctt atctttctag ggctgcttta cttctttagc tatcttggtc agggtcaagg
     2041 aagctttatc tataatgaat tttaacttga aggagaatcc ctattgtgtc aaataaacca
     2101 atagcagata tgattgaaac cattgagcat tttgctcaga cacagcctag ctatcctgtc
     2161 tataatgttt tggggcagga acacacttat ggcgatttaa aggctgattc ggatagtttg
     2221 gctgcagtca ttgaccaact aggcttgcct gagaagtctc ctgtggttgt ttttggtggc
     2281 caagaatatg aaatgttggc aacctttgta gcgctgacta agtcaggtca tgcctacatt
     2341 ccaattgata gccattcggc cttggagcga gtttcagcta ttttagaagt agcagagcca
     2401 agcttgatta ttgccatttc agcctttccc ttggagcagg tttctacacc aatgataaat
     2461 ctagctcagg ttcaagaagc ctttgcccaa gggaataact atgaaatcac gcatccagtc
     2521 aagggagatg ataattacta cattatcttt acttctggta cgactggtaa gcctaaggga
     2581 gtgcagattt cacatgataa tctcctcagc tttacaaact ggatgattac ggataaggaa
     2641 tttgcgacac cgagtcgtcc gcaaatgctg gcacagccac cttattcttt tgacttgtct
     2701 gtcatgtatt gggcaccgac cttggcactt ggtggtacgc ttttcactct tccttcagtc
     2761 atcactcagg acttcaagca actctttgcg gctatctttt cattgccaat cgctatctgg
     2821 atatcaacac catcctttgc agatatggcc atgttgtctg aatacttcaa cagtgagaaa
     2881 atgcctggaa tcacgcattt ctactttgat ggtgaagaat tgacggtcaa aacagctcaa
     2941 aaactgcgcg agcgtttccc aaatgcccgt atcatcaatg cttacggccc aacagaagcg
     3001 acagtagctc tgtcagcagt tgccgtgaca gacgagatgt tagcgactct caaacgccta
     3061 ccaatcggct ataccaaggc tgattctcca acctttatca ttgacgagga aggaaataaa
     3121 ctgccaaatg gcgaacaggg agaaatcatt gtttctgggc cagctgtttc aaaaggttat
     3181 atgaacaatc ctgaaaaaac agcagaagcc ttctttgagt ttgaaggtct gccagcctat
     3241 cacacaggcg atgtgggaac catgacagat gagggcttgc ttctctacgg cggacgcatg
     3301 gacttccaga ttaagttcaa cggttaccgc attgagttag aagatgtctc tcaaaacctc
     3361 aacaagtctc gctttatcga atctgctgtc gcagtaccgc gctataacaa ggaccacaag
     3421 gtacaaaatc tattggctta tgtcatctta aaagacagtg ttcgtgagca gtttgagcga
     3481 gatatcgata ttaccaaggc catcaaggaa gacctgacag acatcatgat gtcctacatg
     3541 atgccatcta aattccttta ccgagacagt ttgccactga ctccaaatgg aaagattgac
     3601 atcaaaggat tgattaacga ggtgaataag agatgatgga gttttttcaa cagcttcctc
     3661 atttagagcc atatggcaat cctcagtatt ttgtttatgt gattgctgca accttgccca
     3721 tctttatagg cctctttttc aagaaacgct ttgcctggta tgaagtgttg gtaagtctct
     3781 tctttattgt caccatgttg gtgggtggaa agaccaatca actagctgcc ttgggtattt
     3841 acctttgctg ggaaatattg ctcctgcttt tctacaagca ttatcgaaaa agcaaggatg
     3901 gcaagtgggt cttctactta gttagttttc tgtccctact tccgattatc tttgtcaagg
     3961 tgcaaccagc tatcaatgga acgcagtctt tgcttgggtt cttgggaatt tcttacctga
     4021 cctttcgttc ggttgggatt atcatcgagc tgagagatgg agtgattaag gattttaccc
     4081 tctgggaatt cctccgtttc cttctcttca tgccaacttt ctcgagtggt ccaatcgatc
     4141 gctttaagcg atttaatgaa aattatcagg ctattcctga gcgagatgag ttgatggata
     4201 tgctggatga atctgtccgc tatatcatgt ggggcttttt gtataagttt atcctagctc
     4261 atgttttagg agagacctta ctacctcctc tgaagaattt agccttgcag tcaggtggct
     4321 tctttaatct ctatgccttg gcagttatgt atacttttgg tctggagctc ttctttgact
     4381 ttgcaggtta ttctatgttt gctttggcca tctcaaactt gatgggaatc cgtagcccta
     4441 tcaactttaa caagcccttt ttatcaaggg atttaaagga gttttggaat cgctggcata
     4501 tgagtctgtc cttctggttc cgtgactttg tctttatgcg aatggtgatg gtgttaacca
     4561 gaaagaaagt ctttaaaaat cgtaatgtaa cctcaagcat ggcctacatt gtaaatatgc
     4621 tgattatggg attttggcat ggtgtgacct ggtactatat cgcctatgga ctctttcatg
     4681 gactaggctt ggtcatcaat gatgcctggg ttcgcaagaa aaaaacgctc aataaggaac
     4741 ggaaaaaagc agggaaggct gccctacctg agaatcgctg gattcagttg cttggcatgg
     4801 ttgtcacttt ccatgttgtc atgttgtcat tcttaatctt ttctggattc ttgaataatc
     4861 tatggtttaa aaaataaaag gaaataaaat atggatatca aatcagaagt tattgaaatt
     4921 attgatgagt tgtttatgga agatgtttct gacatgatgg atgaagatct ttttgatgca
     4981 ggtgtcttgg atagtatggg aacggttgag ttgattgtgg agattgagaa ccgttttgac
     5041 attcgtgtcc ctgtaacaga gtttggtcgt gacgactgga atacagctaa taaaatcata
     5101 gctggtattg tggagctaca aaatgcttaa acgcttatgg atgatcttcg gaccggtctt
     5161 gatcgctggt ttgttggttt ttctgctcat tttcttttat cctactgaga tgcatcataa
     5221 tctaggagct gaaaagcgtt cagcagtggc tactactatc gatagtttta aggagcgaag
     5281 tcaaaaagtc agagcactat ctgatccaaa tgtgcgtttt gttcccttct ttggctctag
     5341 tgaatggctt cgttttgacg gtgctcatcc tgcggtatta gctgagaaat acaatcgttc
     5401 ctaccgtcct tatcttttag gacagggggg agctgcatcg cttaaccaat attttggaat
     5461 gcaacagatg ttaccacagc tggagaataa acaagttgtg tatgttatct cacctcagtg
     5521 gttcagtaaa aatggctatg atccagcagc cttccagcag tattttaatg gagaccagtt
     5581 gactagtttt ctgaaacatc aatctgggga tcaggctagt caatatgcag cgactcgctt
     5641 actgcaacag ttcccaaacg tagctatgaa ggacctggtt cagaagttgg caagtaaagg
     5701 agaattgtcg acagcagaca atgaaatgat tgaattattg gctcgtttta atgaacgcca
     5761 agcctccttt tttggtcagt tttcggttag aggctatgtt aactacgata agcatgtagc
     5821 taagtattta aaaatcttgc cagaccagtt ttcttatcaa gcaatagaag atgttgtcaa
     5881 agcagatgct gaaaaaaata cttccaataa cgagatggga atggaaaatt atttctataa
     5941 tgagcagatc aagaaggatt tgaagaaatt aaaggattct cagaaaagct ttacctatct
     6001 caagtcgcca gagtataatg acttgcagtt ggttttaaca cagttttcta aatctaaggt
     6061 aaacccgatt tttatcattc cacctgttaa taaaaaatgg atggactatg ctggtctacg
     6121 agaggatatg taccaacaaa cggtgcagaa gattcgctac cagttagaaa gtcaaggttt
     6181 taccaatata gcagattttt ctaaggacgg cggggagcct ttctttatga aggacaccat
     6241 tcaccttggt tggttgggtt ggttggcttt tgacaaggca gttgatcctt tcctatccaa
     6301 tcccacacca gctccgactt accatctgaa tgagcgcttt ttcagcaaag attgggcgac
     6361 ttatgatgga gatgtcaaag aatttcaata gataatataa tatagaagag ttcaagaatg
     6421 aaactagttt tcacgttttt tcttgactct ttttttgctt gtgatataat taactggtaa
     6481 acaaaatgtc aaagaatagt atttttctct taaaaaagag aagtctaaaa ggaaaggagt
     6541 cagatatgag acagctagca aaggatatca atgctttttt gaatgaggtg attttgcagg
     6601 cggaaaatca gcatgaaatc ctaataggtc attgcactag cgaggtggcc ctgaccaata
     6661 ctcaggagca tatccttatg ctcttgtcag aggaatcttt aacaaattca gaattggccc
     6721 gtcgtctcaa tgtcagtcag gcggcagtta ccaaggccat taagtctttg gtcaaggaag
     6781 ggatgttgga aacatctaaa gattctaaag atgcgcgtgt gattttttat cagttgactg
     6841 acttggctcg tccaatcgct gaggagcacc accatcacca tgagcataca cttttaacct
     6901 atgaacaagt ggcgactcag tttactccaa atgaacaaaa agtgattcag cggtttttga
     6961 ctgctttagt aggagaaatc aaataatgag atatattacg gtagaggatt tgtccttcta
     7021 ttatgataag gagcctgttc ttgaacatat caattatagt gttgatagtg gggaatttgt
     7081 taccttgact ggggaaaatg gagcggctaa gacgacgctc atcaaggcta gtcttggaat
     7141 tctgcaacca cgcattggaa aggtggctat ttcaaagaca aatacgcaag gtaagaaatt
     7201 gagaatagcc tatcttcctc aacaaattgc cagttttaat gctggttttc caagtacagt
     7261 ctatgaattt gtcaagtcgg gtcgctatcc gagaaaaggc tggttccgtc gtttgaatgc
     7321 tcatgatgag gagcatatca aggccagtct ggactcagtt ggcatgtggg aacatcgaga
     7381 caaacgcttg gggtctctat ctgggggaca aaagcagcga gcggtgattg cgcgtatgtt
     7441 tgcttctgac cctgatgtgt ttatcctaga cgagccgaca acggggatgg atgcaggaag
     7501 taaaaatgaa ttttacgaac tcatgcacca cagcgcccat catcatggca aggctgtttt
     7561 gatgattacc catgaccctg aagaagttaa ggattatgcg gatcgcaata ttcatctagt
     7621 ccgtaaccaa gactcgccat ggcgttgttt caatgttcat gagaatggcc aggaggtggg
     7681 ccatgcttag tttattatct tacgacttta tacaacgcgc ctttttggcg gttattgcta
     7741 tgagtctttt ctcaccggta ttgggaacct tcctcatctt gcgtcgtcag agtttgatga
     7801 gtgataccct tagccacgtc tcactttcag gtgtagcctt tggtctggtt ttggggattt
     7861 ctccaactgt ttctactatt gccattgtct tgattgcggc ggtctttctg gagtatctcc
     7921 gtacggttta caagagcttt atggaaatcg ggacagctat cctcatgtca acaggtctgg
     7981 ctgtttctct gattgtcatg agcaagggta aaagctcgag ttcaatgagt ttggaccaat
     8041 atctctttgg ttcgatcgtg actatcagtg aagaacaggt catttccctc tttgtcattg
     8101 cggcggttgt tttgattttg acctttctct ttcttcgtcc tatgtatatc ttaacttttg
     8161 acgaagatac ggcctttgtg gatggcttgc cagttcgtac catgtccatt ctttttaaca
     8221 tggtgacagg ggtggctatt gcccttatga ttcctgcagc aggagctctt ctggtatcga
     8281 ccattatggt cttgccagct agtattgccc tgcgtctggg gaaaaacttt aaatcggtta
     8341 tgctgcttgc cagtgcgatt ggctttttgg gaatggtagc aggactttac atttcctact
     8401 atgcagaaac acctgcaagt gcaagtatta ccattatttt tgtaactgtc tttatactaa
     8461 tcagtttagt aagacgtttt atcaaatagg agacgaacgt gaaaaaaatt agcttattac
     8521 tagccagtct atgtgccttg tttttagtgg cttgttccaa tcaaaaacag gcagatggta
     8581 aactcaatat cgtgacaacc ttttaccctg tctatgaatt taccaagcaa gtcgcaggag
     8641 atacggctaa tgtagaactc ctaatcggtg ctgggacaga acctcatgaa tacgaaccat
     8701 ctgccaaggc agttgccaaa atccaagatg cagatacctt cgtttatgaa aatgaaaaca
     8761 tggaaacatg ggtacctaaa ttgctagata ccttggataa gaaaaaagtg aaaaccatca
     8821 aggcgacagg cgatatgttg ctcttgccag gtggcgagga agaagaggga gaccatgacc
     8881 atggagaaga aggtcatcac catgagtttg acccccatgt ttggttatca ccagttcgtg
     8941 ccattaaact agtagagcac atccgcgaca gcttgtcagc agattatcct gataaaaaag
     9001 agacctttga gaagaatgca gctgcctata tcgaaaaatt gcaagccttg gataaggctt
     9061 acgcagaagg tttgtctcaa gcaaaacaaa agagctttgt gactcaacac gcagccttta
     9121 actatcttgc cttggactat ggactcaaac aagtcgcaat ctcaggcctt tctccagatg
     9181 cagagccatc agctgctcgc ttggcagaat tgacagagta tgtcaagaaa aataaaatcg
     9241 cctatatcta ttttgaagaa aatgcctcac aagcccttgc taacacactt tcaaaagaag
     9301 caggtgtcaa aactgatgtc ctcaatcctt tagaaagtct gacagaagag gacaccaagg
     9361 ctggagaaaa ctacatttcc gtgatggaga aaaacctcaa ggctttgaaa caaacaacag
     9421 accaagaagg cccagcaatc gaacctgaaa aggcagagga taccaagaca gtccaaaatg
     9481 gttacttcga ggatgcagct gtcaaggacc gcaccttgag tgactatgca ggtaactggc
     9541 aatcagttta tcctttcctt gaagacggca cgtttgacca agtctttgac tacaaggcta
     9601 agttgactgg taagatgacc caggctgagt acaaggctta ctatacaaaa ggctatcaga
     9661 cagatgtgac taagattaac attactgata atactatgga atttgttcaa ggtggacaaa
     9721 gcaagaaata cacttacaag tatgtcggta agaaaatttt gacttacaag aaaggcaatc
     9781 gtggcgtgcg tttcctcttt gaagccacag atgctgacgc tggacagttc aagtatgttc
     9841 agtttagtga ccacaatatt gccccagtta aggcagaaca tttccatatc ttctttggag
     9901 gcacaagcca agaagctcta tttgaagaaa tggataattg gccaacctac tatccagata
     9961 acctatctgg ccaagaaatc gctcaagaaa tgttggcgca ttgatgagaa atcgactagt
    10021 tcataagagc tagtcggttt tttgatacta gatggctaac ttcattatag acctttcaga
    10081 actttaaaca ataaataaaa ctcttgaaat atctgggctt atatgctaga atgtagttag
    10141 tgtataaggt tcattagaac accaaagccc ctaactagtg ccatagttaa gggctttttt
    10201 gacgtatctt agcgatttaa cgggtgttta taggctagtc agtcggtcta cttcttacca
    10261 tctagccatt tgcagacgaa atacaagata attccagcta taacatctgc tataatagta
    10321 agagcaagct ctggtataag gctcattagt ttcacctcct ctcacgaacc cataggaacg
    10381 taatcggtaa ccgatgacaa aagtattata tcacactaaa tttagatcat caacctcact
    10441 taatgcttga actattgtag attatatgct aaaatgtagt taattgataa ggttcattag
    10501 aacaccaaag cccctaacta tggccgtagt taagggcttt tttgacgtat cttagcgatt
    10561 taacgggtgt ttataggcta gtcactcggt ctacttctta ctatctagcc atctgcagac
    10621 gaaatacaag ataattccag ctataacatc cgctataata gtaagagcga gctctatgat
    10681 aaggctcatt agtttcacct cctctcacga acccatagga acgtaatcgg taaccgatga
    10741 caaaaatagt ataccacaat acatttagat catcaaggtc acttaattct tgaaatatag
    10801 ggtctaggcg acaaagtctt taaaatcaga aaaacgcata atatcaggtg tgcaaaaact
    10861 tgatactatg cgttttattg tgggaaggtt tactccattt tctcctgaaa ttgagttttt
    10921 gtccagcctc tgtttttagg gttgctgaga aaataatgtc atgtggtgaa tatttgtaaa
    10981 tcagtcagca gacagaacga atactcttcg aaaatctctt cacatcatgt cagcttcgtc
    11041 tttccgtata tatgtgactg acttcatcag ttctatctac aacctcaaaa cagtgtttcg
    11101 agctgacttc atcagttttc aaatctgtac tttgagcaag ctgagactag cttcctattt
    11161 gattttcatt gaatatcaga aacccattct ccatcaaata attcgactgc gtctaataat
    11221 ttttgatctg gcacggtgtc tgaaataaag gttgtgtatt tggagagggg attaatttta
    11281 aaaaatccag tcttgtaaaa tttagaacta tcaatcagta agatggtttc atgggctttg
    11341 tcaataatat tcttttttga aatagcttgg ctgagagaag cttcataaac atattggtca
    11401 tcaatacctc ttgctgaaca aaatgctaaa tcgatattaa aatgatctaa taaagaattt
    11461 tccttatcat agttgaccac ggaacaggat tgatgtttga cctcgccaga tgtgataaag
    11521 attttggagc tatctttaac agtttcagat agggtttgtg cagtatgtaa accatttgta
    11581 aaaataatca aattatcaag ttcagaaaga tagggacaaa gttcgtagac agtagtactg
    11641 gaatccagat aaacacacat accagaccga ataaagtctt tagcgagact agcgattagt
    11701 cttttttgcc tagtactttc tccttcccgt atttgatgag aaagttcaat tgtgttcata
    11761 gaggacaggg tcacgtatcc gtgctttctt ttgataagac cttgattttc taagaaaatt
    11821 aaatcacgac gtaaggtact tgtgctggag aaagtgattt ctgccagctc ttttacggca
    11881 attctttttt tctttttgat aatttcaatc aattcaagta cacgttcatc ttttatcata
    11941 agctcctcct aatttatcat ttcaactata ttatagcaca aattggagga atttgaatta
    12001 tttttatgaa tattgggtta acatttgaac attattcaag taagcgttca catattgaaa
    12061 aaataaaacg tggggattat aataaggtta atcaaggacg aagagagaag aaaaatggaa
    12121 gcggttttag caatagattt aggtgcgact tctggaagag caatcgttgg ttacctttct
    12181 gaaaataaac tagtaatgga agaaataaat cgcttttcta atctacctat tagagtaaaa
    12241 gggcatttat cttgggatat tgactttcta ctagctaaaa ttcttgaaag tatccgcttg
    12301 gctaatacta gttacaagat tttatctatc ggtattgaca catggggagt tgattttgga
    12361 ctgattgata atgaaggtaa gctgttatta caacctgttc attatcgtga tgaaagaaca
    12421 aagggagtgt taaaggaaat atctgaaatg actgaattag aaaaactgta ttcagagaca
    12481 ggaaatcaga ttatggagat aaataccttg tttcaactct ttaaggcacg tcaagaatct
    12541 cctgactctt tctataagac caataagatt cttttaatgc cagatttgtt taattatctc
    12601 ttgacaggta agtttgctac agaaaaaagc attgcttcaa caactcaatt atttgatcct
    12661 aggagtcaaa attggaatca gaatatctta aaactatttg aattggattc aactttactt
    12721 cctgaaattg tttcagaggg aaatgtcctt ggaaggataa aagaggagta tggtttaggc
    12781 gatattcctg ttgtgaatgt ttgtagtcat gatacagcaa gcgcgattgt ctcagtacct
    12841 aagacagaag atagtttgtt tatttcatca gggacttggt ctttggttgg agtggagctt
    12901 tcttcaccta ttcttagtac cgaatccttc agttatggat ttacaaatga agtcggtaaa
    12961 gatggagtga ttacatttct gaagaattgt acagggttgt ggatcataga ggaactaaga
    13021 cgttcatttg aacgaagagg gaaagcctat tcttttgatg atattaggac aatggtggag
    13081 aaagaaaaag aaaatcttcc tctgattgat actgaatcaa ctgaatttgc aacagaatct
    13141 gatatgcaca agactttgac agaatatcta gcttatcatc atgaaactgg agagtggaca
    13201 gatggacaac tatttaagat cgtttatgaa agcctagctg aaacgtataa ggaaacaata
    13261 gagttactag aagaactaac tcataaggtt tataagagga tatatgtgat tggaggaggt
    13321 gctagagcca gttactttaa ccaaatgatt gctgatagaa ctggtaaaga agttcttaca
    13381 ggttcaactg aggctacagc tgtggggaat attgttgtgc agctcatagc tatgggacaa
    13441 ttaaaaggga tggaagaagc tcaccatgtt attgaggagt tcctacaatt agagagttat
    13501 tactcccaaa agaattaaaa agattgagag tttgtaaatt tgcctccctc ccccttctta
    13561 agttttgtac aagaaggggg gataattggt gaattgaaaa atatttagtg ttttgatatg
    13621 aggaggacaa gaatgtcaga tgtaaaacaa gaattaatta agtatggcaa gaagctagta
    13681 gaaacagatt tgacgaaagg aacaggtggg aatctcagtg ttttcgatcg tgaaaaacaa
    13741 ttgatggcaa ttaccccgtc gggtattgat ttctttgaaa tcaaagaatc cgatattgta
    13801 gtgatggata ttaatggaaa tgttgtagag ggagaacgct tgccatctag cgaatggtat
    13861 atgcatttga ttcaatatca aactcgtgat gatatcgatg caattatcca tgctcataca
    13921 acttatgcaa cagtattagc ttgtctcaga gaaccacttc cggctagtca ttatatgatt
    13981 gcagtggcag ggaaagatgt tcgggtagct gagtatgcaa catatggtac gaaagaattg
    14041 gctgtgaatg cagctaaagc aatggaaggt cgtagagcag ttttactagc gaatcatgga
    14101 attttagcag gtgcacaaaa tttattgaat gcatttaata ttgttgaaga agttgaatat
    14161 tgtgcaaaaa tttattgttt agctaagaat tttggagagc cagtagttct tcctgatgag
    14221 gagatggatt taatggcaga aaaatttaaa acatacggtc agagaaaata gggaggatat
    14281 taatgttaaa acatataccg aaaaatattt ctccagattt attgaagact ttaatggaaa
    14341 tgggacatgg agatgaaata gtattagctg acgcgaatta tccttctgcc tcatgtgcaa
    14401 ataagctaat tcgttgtgat ggtgtaaata ttccagaatt attagattcc attctgtatt
    14461 taatgccatt agatagttac gtcgatagtt caattcagtt tatgaacgtt gtttcgggtg
    14521 atgatattcc taagatatgg ggtacctata gacagatgat tgaaggtcat ggtacagatc
    14581 ttaaaacgat tacttatctt agaagagaag acttttatga acgtagtaag aaagcttatg
    14641 ctattgttgc tacaggagaa acttcacttt atgctaatat tatccttaag aaaggagtag
    14701 ttgttgaaag agaaaatgtt taatagagga attttagttg ccagtcatgg taattttgct
    14761 agcggagctc tcatgaccgc agaaatgttt gttggtgaga caacaaatga tagagttagg
    14821 acattaggtt tgatgcctgg agagaatatt gtagagtttg aacattattt taaaaatcaa
    14881 gtaaatgaac tgttagactc aaatcaagag gttatcgttt tgactgactt gattggagga
    14941 agtcctaata atgtggcttt gtcacggttt ttaaatttgg attcagttga tattgtaaca
    15001 gggtttaata tccctctcct agtggaatta atatcaagtt atgattcaaa aatcaattta
    15061 gaagaaattg ttcaaaatgc tcaaaatagt ttgtttaatg ttaaacaaca acttaacgta
    15121 gaggaggaag aggatttatg tctatagagt ttgttcgtat tgatgaccgt ctggtacatg
    15181 gtcaagttgt cactacgtgg ctaaaaaagt atgatattga gcaagttatc attgttaatg
    15241 atcgcatctc agaagataaa acacgacaat ctattttaaa gatttctgca ccggtaggtt
    15301 taaaaattgt tttctttagt gtaaaacggt ttgtggaagt tttaaactct gtgccaataa
    15361 aaaagagaac aatgctgata tatacaaatc caaaagatgt gtatgattct attgaaggaa
    15421 atttaaaatt ggagtacctc aatgtaggac agatgagtaa aacggaggaa aatgaaaagg
    15481 taacgggagg tgtagctcta ggtgaagaag acaaatatta ttttaagaaa atagttgata
    15541 agggaacgag agttgaaatt caaatggttc ctaatgataa agttacaatg ttggaaaaat
    15601 ttttataaaa ataatttaag gaggtacagt atatgctatt cacacaagca ttactggtga
    15661 cattagttgg gattattgcc actattgact ataatggacc gttatttatg attcaccgtc
    15721 cgttagttac aagtgcaatg gttggcttag tcttaggaga tttcacccaa ggtgttctta
    15781 ttggttcagc tcttgaatta acttggctcg gtgtaacagg tattggaggt tacactccac
    15841 cagatactat ttcaggtgcg attattggta ctgcatttgg tattttgtct ggtcaaggag
    15901 aaactgctgg tatcgctata gcagttccaa ttgcagttgc tacccaacag ttggatgttc
    15961 ttgcaaaaac tttagatgtt tattttgtga aaaaagctga taatgatgct aaaaacggag
    16021 attattcaaa gatcggtttt tatcattatt caagtttggt tttaatcact ttatttaaaa
    16081 ttgtaccaat tttcctagct attatgcttg gaggggaata tgtggcagac ttgtttgcta
    16141 aggttccacc aatcgttatg cagggactta actctgcagg tgctttacta ccttcaattg
    16201 gttttggtat gcttttaaat atgatgctca agaaaaatat gtgggtattc ttgttgattg
    16261 gattcatttg ttctgtgtat ggaggaatgt caaccattgg gatctcacta gttggtattg
    16321 cggtagcata cttctacgat atgattggaa gtaaaccaca agaaacaact tcaagtagtg
    16381 atgttgagga ggatcttgat ctatgatgaa taataaagta actaaagttg aacttaaaaa
    16441 agttttcaaa cgaagtttta tgtatggttc ttcatggaac tatgagagaa tgcagaacct
    16501 aggttttcta tatacaattc ttccagtatt gaaaaaacta tacccagaca aagattcagc
    16561 ttctcctgca atgaaacgtc accttgagtt tttcaatact catcaaacag cggcaccatt
    16621 tattcttgga gttacttccg ctatggaaga acaagaagga aatgaaggtg cagcttcaat
    16681 tactggtatt aaagttggct tgatggggcc gctggctggt ctaggagata gtttgttctg
    16741 gctgacacta gttcctatct gttttagtat tggtgcgtct tattctaaag acggcggtgc
    16801 tttaggtatc tttatcgcct taatattgtt taatattatt aatattcctg ttaaatattt
    16861 cggtttgaaa tatgggtata ctaagggttc tagtcttatc caagaaaata atacaaaagg
    16921 aacattgaat cgcgttacga gtatggcgac agcattaggg ctagtactag tgggtggttt
    16981 gattccatca atggttggta ttaattttgg attagaattt aagcaggggg aacttgttat
    17041 ttctgttcaa gaaatgatta caaaattaat tccaggattt atccctatgg ctttgacttt
    17101 attaatgtgt aaattaatta gaaaaggaaa gaatccggtt gtactaatct ttagtgttat
    17161 ggctattgga gttatcctag ttgttttagg aattttgaag tagtagaaag tgtggaggtg
    17221 gtatttggga tatcacctcc attttggaag agaggtaaag agtgaaatta tggtataaga
    17281 aagctgcctc aaattggaat gaagccttgc cgattgggaa cggtcattta ggtggtatga
    17341 tttatggttc agctacaaaa gaatgtattc aactaaacga tgagactatt tggtatagag
    17401 gaaagtcaga tagaaataat ccagactcac tattgcatct taaaaaaatt cgggaatatc
    17461 ttttagatgg agaaattcag aaagccgaag aattgataaa gttaacaatg tttgctaccc
    17521 caagagatca aagccactat gaattacttg gggaacttta cattgagcat atagatattc
    17581 agtcttgtgc tctttcattg tatgaaagag agctagattt agatacagct atttctaatg
    17641 ttgtgtttga gcctaatagt tgtaatttac aaataaaaag agaatatttt acgagtttta
    17701 ataagaatat tttatgttgc cgtatagtgt catcagttca aaacacatta aatttaaaca
    17761 ttaatttggg tagaaataaa cggtttaatg atgaagtatc taaactggat tcaagtacaa
    17821 ttttaatgtc ggcctctgct ggaggtagaa aaggtgttca gtttaaagtg gtatgtcatt
    17881 ctaaggttac ggatggtgaa gtaagtgtat tgggagagac aatagttatt cggaatgcta
    17941 cagaggtatt tctttatctc aaatcaatga cggattattg gggaaatata gatatttctt
    18001 ctcttcaggg agaatttagt agtattgatt actttacaga aaaagatgaa catgtaaaaa
    18061 aatatcagga gcaatttaat agagttgatt ttaaactaga ctatagtaaa gattgtctta
    18121 gcattccaac gaatctactt cttgaaaaca ctaaaaagta tagtaactac ttgactaact
    18181 tgttatttca ttatggaaga tatctgttaa tatcgtctag tcaaccgaat ggtttacctg
    18241 ccaatcttca aggaatatgg tgtgatgaat taaatccaat ttggggttct aaatatacga
    18301 ttaatattaa tactcaaatg aattattgga tggtaggtcc atgtgattta ccagaagtag
    18361 aatatccatt atttgatatg ctcgaaagaa tgagagaacc gggaagacta accgctaaga
    18421 aaatgtatgg agctagaggt tttacagcac atcataatac ggatggtttt ggcgatacgg
    18481 ctccccaatc tcatgccatg ggggctgcaa tttgggtatt aactattcca tggttatgta
    18541 ctcatatttg ggaacactat ttatatttcc aagatgagcg tattcttacg gaacattttg
    18601 aaatgataaa agaagcattt cttttctttg aagattattt atttgaggtg gatggctact
    18661 tgatgacagg tccaagtgtc tcaccggaaa ataaatatcg cttaaaaaat ggtattgaag
    18721 gaaatgcttg tctatcatct acaattgata atcaaatttt aagatatttt tgtgattcat
    18781 gcattggaat tgcaaaacaa ttaggagaca attcggattt tattagtcgt gtgaaggagt
    18841 taaaaaagaa attacctaga acaaaaatag gtagtaatgg gcaaatacaa gaatggctag
    18901 aggattatga agaagtagag cctggtcata gacacatttc acctctattt gggctttatc
    18961 cttataatga gattgatatt cataaaactc cggaattagc agaagcagct aaaatcacta
    19021 tcaataggag attatcaaac gctaactttt tatcttcaca ggatagggag caagcgatta
    19081 ataattggtt agtaagtggt ttgcatgcta gtacacaaac aggttggagt gctgcatggc
    19141 tgattcattt ttttgcgaga ctatatcaag gtgaacctgc ttataaccag attaatggtt
    19201 tgttaaataa tgcgactctt ggcaatttat ttcttgacca tccaccattt caaattgatg
    19261 gtaatttagg tttggtgagt ggaatttgtg aattattagt acagagccat cataattggt
    19321 tatcactaat tccagcttta ccttctgctt ggtcagaagg agaagtgaaa ggtttcagag
    19381 taagaggagg atataaggta tcgtttgctt ggaaaaatgg ggatataaca ttcctaaaat
    19441 tggaaggagg aaacaaagat caaaaagtaa gagtaagaat atatggcaaa aatactgatg
    19501 tacaaaatat tgaattggta tttaattcag aaaaaattat tgagttaaat ttttaggtat
    19561 aagtcatgaa taaagaaaaa ataaaaagaa aattaatcac aatactgttt gtatgtattg
    19621 ggatgttatg ttttggattg ttagcaggag ttaaggctga taatcgtgtt caaatgagaa
    19681 cgacgattaa taatgaatcg ccattgttgc tttctccgtt gtatggcaat gataatggta
    19741 acggattatg gtgggggaac acattgaagg gagcatggga agctattcct gaagatgtaa
    19801 agccatatgc agcgattgaa cttcatcctg caaaagtctg taaaccaaca agttgtattc
    19861 cacgagatac gaaagaattg agagaatggt atgtcaagat gttggaggaa gctcaaagtc
    19921 taaacattcc agttttcttg gttattatgt cggctggaga gcgtaataca gttcctccag
    19981 agtggttaga tgaacaattc caaaagtata gtgtgttaaa aggtgtttta aatattgaga
    20041 attattggat ttacaataac cagttagctc cgcatagtgc taaatatttg gaagtttgtg
    20101 ccaaatatgg agcgcatttt atctggcatg atcatgaaaa atggttctgg gaaactatta
    20161 tgaatgatcc gacattcttt gaagcgagtc aaaaatatca taaaaatttg gtgttggcaa
    20221 ctaaaaatac gccaataaga gatgatgcgg gtacagattc tatcgttagt ggattttggt
    20281 tgagtggctt atgtgataac tggggctcat caacagatac atggaaatgg tgggaaaaac
    20341 attatacaaa cacatttgaa actggaagag ctagggatat gagatcctat gcatcggaac
    20401 cagaatcaat gattgctatg gaaatgatga atgtatatac tgggggaggc acagtttata
    20461 attttgaatg tgccgcgtat acatttatga caaatgatgt accaactcca gcatttacta
    20521 aaggtattat tcctttcttt agacatgcta tacaaaatcc agctccaagt aaggaagaag
    20581 ttgtaaatag aacaaaagct gtattttgga atggagaagg taggattagt tcattaaacg
    20641 gattttatca aggactttat tcgaatgatg aaacaatgcc tttatataat aatgggagat
    20701 atcatattct tcctgtaata catgagaaaa ttgataagga aaagatttca tctatattcc
    20761 ctaatgcaaa aattttgact aaaaatagtg aggaattgtc tagtaaagtc aactatttaa
    20821 actcgcttta tccaaaactt tatgaaggag atgggtatgc tcagcgtgta ggtaattcct
    20881 ggtatattta taatagtaat gctaatatca ataaaaatca gcaagtaatg ttgcctatgt
    20941 atactaataa tacaaagtcg ttatcgttag atttgacgcc acatacttac gctgttgtta
    21001 aagaaaatcc aaataattta catattttat tgaacaatta caggacagat aagacagcta
    21061 tgtgggcatt atcaggaaat tttgatgcat caaaaagttg gaagaaagaa gaattagagt
    21121 tagcgaactg gataagcaaa aattattcca tcaatcctgt agataatgac tttaggacaa
    21181 caacacttac attaaaaggg catactggtc ataaacctca gataaatata agtggcgata
    21241 aaaatcatta tacttataca gaaaattggg atgagaatac ccatgtttat accattacgg
    21301 ttaatcataa tggaatggta gagatgtcta taaatactga agggacaggc ccagtccctt
    21361 tcccaacacc agataaattt aatgatggta atttgaatat agcatatgca aaaccaacaa
    21421 cacaaagttc tgtagattac aatggagacc ctaatagagc tgtggatggt aacagaaatg
    21481 gtaattttaa ctctggttcg gtaacacaca ctagggcaga taatccctct tggtgggaag
    21541 tcgatttgaa aaaaatggat aaagttgggc ttgttaaaat ttataatcgc acagatgctg
    21601 agactcaacg tctatctaat tttgatgtga ttctatatga caataataga aacgaagttg
    21661 ctaagaaaca tgttaataat ttgtcgggtg aatctgttag tctagatttc aaagaaaaag
    21721 gagcaaggta tattaaagtt aaattactaa cgagtggagt gcctttgagt ttagcagaag
    21781 tagaggtttt tagagaatca gatggtaagc aatctgaaga ggatatagat aaaataacag
    21841 aagataaagt agtctctaca aataaggtag ctactcaaag ttcaaccaat tatgagggtg
    21901 tagctgcttt agcagttgat ggtaataaag atggagatta cggacatcat tcggtgactc
    21961 atactaaggc agattctaac gcttggtggc aggtcgatct gggagaagag tttacggttt
    22021 ctaaagttga tatttataat agaacagatg ccgaacctca gcgtttatct aattttgatg
    22081 ttatttttct atcttcatca ggagaagaag tttttagaag acattttgat aaagtagttg
    22141 atggtttgtt atctttaaaa gtaccttctg taggggctaa gctagtcaaa atagaattaa
    22201 aatcagcagc tattccgtta agtttagcgg aagttgaagt ctatggttca aagagaactc
    22261 cgaagaaact ttctaatatt gcattaacca aagaaactcg acagagttca acggattaca
    22321 atggtttttc tcgtctagca gttgatggaa ataaaaacgg agattatggt catcattcag
    22381 tgactcatac caaaggagat tctccttcat ggtgggagat agatttagca caaaccgaag
    22441 aattagaaaa gttaattatt tataatagaa cagatgctga aattcagata ttatcaaatt
    22501 ttgatattat tatatatgat tcaaataatc atgaagtttt taaacaacat attgacagtt
    22561 tagaaagcaa taatctatcc atagacttaa aagggctgaa gggaaaaaag gttagaattt
    22621 ctttgagaaa cgcaggaatt cctttaagtt tggcagaggt agaggtttat acttataagt
    22681 aattttaaaa attatcatcg gtagtatgaa agaaacagaa aaataagagg aaaatagtat
    22741 gattcaacat ccacgtattg ggattcgtcc gactattgat ggtcgtcgtc aaggtgtacg
    22801 cgaatcactt gaagtacaaa caatgaacat ggctaaaagt gtggcagatt tgatttcaag
    22861 cacattgaaa tatccagatg gggaacctgt ggaatgtgtg atttctccat ctaccattgg
    22921 tcgtgttcca gaggctgcag cttcccatga gttgtttaaa aaatcaaatg tttgcgcaac
    22981 aattacagtt acaccatgct ggtgttatgg tagtgaaact atggatatgt ctccagatat
    23041 tcctcatgct atttggggat ttaatgggac agaacgccca ggagctgtct atcttgcagc
    23101 tgtactagct tcacatgctc aaaaagggat tccagccttt gggatttatg gtagagatgt
    23161 tcaggaagct aatgatacag atattccaga agatgtcaaa gaaaaacttt tacgttatgc
    23221 gcgtgcagct cttgcaactg gcttgatgag agacactgct tacctatcaa tgggtagtgt
    23281 ttcgatgggg attggcggtt ctattgtaaa tccagatttc ttccaagaat acttaggaat
    23341 gcgaaatgaa tcggtagata tgacggagtt cacgcgccgt atggaccgtg gtatttatga
    23401 cccagaagag ttcgaacgtg ctatggtttg ggtaaaagaa catatcaaag aaggggttga
    23461 ccgaaatcgt gaggatttga ttctttctaa agaagagaaa gaaaaacaat gggaatttgt
    23521 tattaagatg ttcatgattg gacgtgactt aatggttggt aacccaagac ttgctgaact
    23581 tggttttgag gaagaagcgg ttggtcacca tgctttagta gctggtttcc aaggtcaacg
    23641 tcagtggaca gaccattttc caaatggaga ttttatggaa actttcctca atactcagtt
    23701 tgactggaat ggtattcgaa aaccatttgt atttgcgaca gagaatgatt cactaaatgg
    23761 tgtgtctatg ctctttaatt atctattaac aaatactcca caaatctttg ctgatgtgcg
    23821 tacttattgg agcccagagg ctgttaaacg tgtaacggga catactttag agggtcgtgc
    23881 tgcagctggc ttcttacatc taatcaactc tggttcttgt acattggatg gtacaggtca
    23941 agctactcga gatggcaaac ctgttatgaa accattctgg gagttggaag aaagtgaagt
    24001 gcaggctatg cttgaaaata cagacttccc accagcaaac cgcgaatact tccgtggagg
    24061 aggattctca actcgtttct tgacgaaggg ggatatgcca gtaacaatgg tacgtctcaa
    24121 tcttctaaaa ggggttggtc cagtgctaca aattgcagaa ggttatacac ttgaacttcc
    24181 tgaagatgtt caccatactt tagataatcg tacagatcca ggatggccaa ctacttggtt
    24241 tgctccacgt ttgacaggaa aaggtgcttt caagtctgtc tatgacgtca tgaataattg
    24301 gggagctaat cacggagcca taacatatgg acacattgga gcagacttga ttaccttggc
    24361 ttctatgttg agaattcctg tcaatatgca taatgtacct gaggaagata tctttagacc
    24421 taaaaattgg tccttatttg gaacagaaga tctagaatca gcagactatc gtgcatgtca
    24481 gttgttggga ccactacata aataaaactt gtttatatag gaggtgaact tacgtccctc
    24541 ctatcctttt aaaaagattt gttaaacaat tcacaaataa ttgaaaacga atacaaaaag
    24601 taatataatg atgttaaata gatagcgcaa aggcgcagga ggaaaattat atggctacat
    24661 tttatgttcc ggcagtcaac cttattggta aaggtgttgt aaatgaagtg ggtccttata
    24721 tcaaggaact tggctataaa aaggcacttt tggtgacaga taagtacatc gaaggcagtg
    24781 acattttacc taaggtttta aaaccgctag atacagaagg aatcgaatat gtcatcttta
    24841 gggatgtaga gccaaaccct acttgtaaaa atgtcacaga tggggtagct gctttgcaag
    24901 aacatggatg tgattttatc atcagtcttg gtgggggttc tccacaggat gcagctagtt
    24961 gtatttctat catggctaca aatggtggaa gaccacagga ttatgaaggg cttcataagt
    25021 ctgctaaaaa aggcttgcca gttgtggcta tcaatacaac ggcaggaact tctgcagaaa
    25081 tcactattaa ctatgtgatt actgatgaag aacgcaaggt taagatggta atggttgaca
    25141 agaatagcct tgctcttatc tctgtcaatg acccggaact catgctttcc aaacctcaag
    25201 ccttgactgc tgctactggt atggatgctc tgactcacgc tgttgaagct ttggtaacac
    25261 ctggtgctta tgatgtaacc aagaaactgt ctattggtgc tattgagctt atcaaggaat
    25321 atcttcctcg tgctgtagaa aatggacatg atattgaagc gcgtgaaggt atggtcaatg
    25381 ccatcttcct tggtggtatg agctttaata atgctggtct tggttatgtt cactcaatgg
    25441 ctcaccaact cggtgcagta tataatttac cacatggtgt ttgctgtgcc atgttgctac
    25501 cagttataga acgtgaaaat gctaaacgtg taccagaagc tttccgcaat gttgccaaag
    25561 ccttgggact tcatgtagaa ggtaaatcag atcaagaatg tgccgattat gcgattgctg
    25621 agattgagaa gctttctgag acagtaggta ttcctaagaa actgactgaa cttggtattg
    25681 aagaaaaaga tttcgacttt gaataccttt ctaagaatgc cttgattgat gcctgcgcac
    25741 caggaaatcc atttatgcca accttagaag aaacgattgc cttttataaa gagttatttt
    25801 agcaagagat tggacgacaa taggacaatt cacctctaat tattgacatt tcaggaaaaa
    25861 tcgcagtaaa aacggcacag ccttctttaa atgtgctata atactagaaa aatacttgtg
    25921 gaggttccat tatggcaata tttttcatga tttttctgat tgtttgtgtg ctcctattgg
    25981 tgatagtcac actgagtaca gtttatgtgg ttcgtcagca gtcggtggcg attattgaac
    26041 gctttgggaa ataccaaaag gttgctaata gcggtattca tattcgcttg ccttttggga
    26101 ttgactcgat tgcagcacgg attcagttgc gcttgttgca aagtgatatt gtggttgaga
    26161 ctaagaccaa ggacaatgtg ttcgttatga tgaatgtagc gactcagtac cgtgtcaacg
    26221 agcagagcgt gacagatgct tactataaac tcatacgtcc agaatctcag attaaatctt
    26281 atatcgaaga tgctcttcgc tcttctgttc caaaattaac cttggatgaa ttgtttgaga
    26341 aaaaagatga gattgccctt gaagttcaac accaagtagc agaagaaatg accacttacg
    26401 gctacattat cgtgaaaacc ttgattacca aagtcgaacc ggatgcagaa gttaagcaat
    26461 ccatgaatga aatcaatgcg gcgcaacgta agcgggtcgc agcacaagaa ttggcggaag
    26521 ctgacaagat taaaattgtc actgcagctg aagcagaagc agaaaaagac cgccttcatg
    26581 gtgtggggat tgcccaacaa cgtaaggcga ttgtggatgg attggcagag tctatcaccg
    26641 aactcaagga agccaatgtt ggcatgacag aagaacaaat catgtctatc ctcttgacca
    26701 accagtattt ggataccttg aatacctttg cctctaaagg aaatcaaacc atctttttac
    26761 caaatacgcc aaatggtgtg gatgatatcc gaacacaaat cttgtcagcc cttcgcgctg
    26821 agaagaaata atagactaat actcttcgaa aatctcttca aaccacgtca gcgtcgcctt
    26881 gccgtatatt tgagctgact tcgtcagttc tatctgcaac ctcaaaacag tgttttgagc
    26941 tgacttcgtc agtcttatct actacctcaa aacggtgttt tgagcaacct gtggctagtt
    27001 tcctagtttg ctatttgatt ttcattgagt acaaagagct tggcaacagg ctctttttgt
    27061 attgcgttta ttaagaaaaa caaaaaacat agatatacat ttaactaaaa attattgtat
    27121 gttacctccc ttaattagga atgataaggg aataactaga aagatttgtg aatacaaact
    27181 atttctgata tacaaaatat acagtaataa tgaatgatgg gagatgggat gaaagaattt
    27241 caatttgaga gaaagcagcg tttttctttg aggccatatg caataggagc ttgttcggtc
    27301 ttgctaggaa cgagtttatt ttttgctggt atgggtgatc agcctgtaca ggatacagaa
    27361 acgagttcag cactaatttc aagtcattat ttggatgagc aggatttatc tgaaaagctg
    27421 aaatctgagt tgcaatggtt tgaattagaa aacaagcttt tgaacttatg ggagcattag
    27481 gttactatga aggatttgtt ccttatgttt caaattaata caaaaaccaa gctgaagaag
    27541 aagacaaacc gctatctgat aaatatattt ttgaaaaaat cttaggaaaa acatatgcag
    27601 ccttcaaaaa agatcaaatt aatgagcgtg tcgagaaatt aggtaagtta aaacctatta
    27661 caataaatta caacggaaaa tcagaagtaa ttgatagtaa agaaaaatta caagagctta
    27721 tgaataaagc cgttaaagac gaagtggctc aaatatagta aaatgaaata agaa
//
LOCUS       DAVMGZ010000012        26705 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000012 DAVMGZ010000000
VERSION     DAVMGZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26705
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.012"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            60..608
                     /locus_tag="VYQ93_000433"
     CDS             60..608
                     /locus_tag="VYQ93_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV3335177.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNSTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            718..1617
                     /locus_tag="VYQ93_000434"
     CDS             718..1617
                     /locus_tag="VYQ93_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV3335178.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDMLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPERRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(1642..2916)
                     /gene="serS"
                     /locus_tag="VYQ93_000435"
     CDS             complement(1642..2916)
                     /gene="serS"
                     /locus_tag="VYQ93_000435"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV3335179.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3130..3501)
                     /locus_tag="VYQ93_000436"
     CDS             complement(3130..3501)
                     /locus_tag="VYQ93_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV3335180.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGCKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3504..4868)
                     /locus_tag="VYQ93_000437"
     CDS             complement(3504..4868)
                     /locus_tag="VYQ93_000437"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV3335181.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            5238..6023
                     /locus_tag="VYQ93_000438"
     CDS             5238..6023
                     /locus_tag="VYQ93_000438"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV3335182.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6117..6551
                     /locus_tag="VYQ93_000439"
     CDS             6117..6551
                     /locus_tag="VYQ93_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV3335183.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6551..7525
                     /locus_tag="VYQ93_000440"
     CDS             6551..7525
                     /locus_tag="VYQ93_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV3335184.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7585..7809
                     /locus_tag="VYQ93_000441"
     CDS             7585..7809
                     /locus_tag="VYQ93_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV3335185.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            7928..8902
                     /gene="fabK"
                     /locus_tag="VYQ93_000442"
     CDS             7928..8902
                     /gene="fabK"
                     /locus_tag="VYQ93_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV3335186.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            8895..9815
                     /gene="fabD"
                     /locus_tag="VYQ93_000443"
     CDS             8895..9815
                     /gene="fabD"
                     /locus_tag="VYQ93_000443"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV3335187.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSNFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            9849..10580
                     /gene="fabG"
                     /locus_tag="VYQ93_000444"
     CDS             9849..10580
                     /gene="fabG"
                     /locus_tag="VYQ93_000444"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV3335188.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            10602..11837
                     /gene="fabF"
                     /locus_tag="VYQ93_000445"
     CDS             10602..11837
                     /gene="fabF"
                     /locus_tag="VYQ93_000445"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV3335189.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEVNVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11840..12325
                     /locus_tag="VYQ93_000446"
     CDS             11840..12325
                     /locus_tag="VYQ93_000446"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV3335190.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12322..12744
                     /gene="fabZ"
                     /locus_tag="VYQ93_000447"
     CDS             12322..12744
                     /gene="fabZ"
                     /locus_tag="VYQ93_000447"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV3335191.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSENTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            12756..14123
                     /gene="accC"
                     /locus_tag="VYQ93_000448"
     CDS             12756..14123
                     /gene="accC"
                     /locus_tag="VYQ93_000448"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV3335192.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14160..15026
                     /locus_tag="VYQ93_000449"
     CDS             14160..15026
                     /locus_tag="VYQ93_000449"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV3335193.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            15023..15790
                     /locus_tag="VYQ93_000450"
     CDS             15023..15790
                     /locus_tag="VYQ93_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV3335194.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            15949..16134
                     /gene="briC"
                     /locus_tag="VYQ93_000451"
     CDS             15949..16134
                     /gene="briC"
                     /locus_tag="VYQ93_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV3335195.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            16152..16625
                     /locus_tag="VYQ93_000452"
     CDS             16152..16625
                     /locus_tag="VYQ93_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335196.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            16638..16874
                     /locus_tag="VYQ93_000453"
     CDS             16638..16874
                     /locus_tag="VYQ93_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3335197.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(16921..17343)
                     /gene="nusB"
                     /locus_tag="VYQ93_000454"
     CDS             complement(16921..17343)
                     /gene="nusB"
                     /locus_tag="VYQ93_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV3335198.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(17336..17725)
                     /locus_tag="VYQ93_000455"
     CDS             complement(17336..17725)
                     /locus_tag="VYQ93_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV3335199.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(17747..18307)
                     /gene="efp"
                     /locus_tag="VYQ93_000456"
     CDS             complement(17747..18307)
                     /gene="efp"
                     /locus_tag="VYQ93_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV3335200.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(18436..19878)
                     /gene="gatB"
                     /locus_tag="VYQ93_000457"
     CDS             complement(18436..19878)
                     /gene="gatB"
                     /locus_tag="VYQ93_000457"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV3335201.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(19878..21344)
                     /gene="gatA"
                     /locus_tag="VYQ93_000458"
     CDS             complement(19878..21344)
                     /gene="gatA"
                     /locus_tag="VYQ93_000458"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV3335202.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKHSEETIYQVAAAFEATTGYHKQQPVIFGGDN"
     gene            complement(21344..21646)
                     /gene="gatC"
                     /locus_tag="VYQ93_000459"
     CDS             complement(21344..21646)
                     /gene="gatC"
                     /locus_tag="VYQ93_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV3335203.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            21859..23403
                     /locus_tag="VYQ93_000460"
     CDS             21859..23403
                     /locus_tag="VYQ93_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV3335204.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            23657..23854
                     /locus_tag="VYQ93_000461"
     CDS             23657..23854
                     /locus_tag="VYQ93_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV3335205.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            23965..24141
                     /locus_tag="VYQ93_000462"
     CDS             23965..24141
                     /locus_tag="VYQ93_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV3335206.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAISLAAVRDDFYTHNFNSGILLIN
                     NGMWRAENVTQDLI"
     gene            24257..24445
                     /locus_tag="VYQ93_000463"
     CDS             24257..24445
                     /locus_tag="VYQ93_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV3335207.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            24602..24967
                     /locus_tag="VYQ93_000464"
     CDS             24602..24967
                     /locus_tag="VYQ93_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV3335208.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            24970..26637
                     /locus_tag="VYQ93_000465"
     CDS             24970..26637
                     /locus_tag="VYQ93_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV3335209.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT         7657 a         5084 c         6172 g         7792 t
ORIGIN      
        1 caggatttta taagattttc caataatatt agtgtcaata ataagaaaag aggtatctta
       61 tgacaacatt tactatccat acagtagagt cagcaccagc agaagtgaaa gaaattcttg
      121 aaacagtaga aaaagacaac aatggctata ttcccaacct aatcggtctc ttggccaatg
      181 ccccgactgt tttagaagcc taccaaattg tctcatctat ccaccgtcgc aacagcctga
      241 cacccgttga gcgtgaagtg gtgcaaatca cggcagccgt gaccaatggt tgtgccttct
      301 gtgtcgcagg tcacacagcc ttttccatca aacaaatcca gatgaatgat gacttgattc
      361 aagctcttcg caatagtact ccaattgaaa cagatcctaa attggatacc ctagctaagt
      421 ttaccttggc agttatcaat accaagggtc gtgtaggaga tgaagccttg tctgagtttt
      481 tagaagctgg ctacactcaa caaaatgcct tggatgtggt tttcggtgtc agcctagcaa
      541 tcctctgtaa ctatgccaac aacttagcta atacaccaat taatccagaa ttgcaacctt
      601 atgcctaatt catatctgag taaaatgaag tctgaaataa tcggacttcg tttttttaaa
      661 tcctcattta agcgctttta tgttatactg aaactaacat gattattgga ggttaggatg
      721 aaaaaactcc ccttagtatt ttcaggttgt ttgctaggtt tggcaggagc tggaaatctt
      781 attttagata tgttgccagt tctatcccat ctttttagtc tgactggttt ggttttatgg
      841 atttactttc taattctgca tctctttaat tggaaagaaa ccaagcaaga attgaccaag
      901 ccccctcttt tgtcaggaat ggcaaccttt cctatggctg ggatgatttt atcgacctat
      961 gtctttcgcg tattctctta tcttcctttg gtagcacaag ggatttggtg gttttcattt
     1021 ctcttggatt tgaccttgat tgctggtttt accatcaagt ttgcttgtcc agagcggagg
     1081 gttcatgcca ctccaagctg gacggttctc tatgtgggga tagcagtggc tgccttgacc
     1141 tatcctctgg taggtattat cgaaattgcc tatgcgacct tgagttttgg ttttctcttg
     1201 accttctatc tctatcccct tatttatagc gatttaaaga aacatccact cccactagcc
     1261 ttgcttggac aagaaggaat ctactgtgct cctttctctc tactcttggc ttctctagtt
     1321 cgagtaggag gaaccagcct gccgacttgg gtcttgattg tcatgatttt ggcttctcaa
     1381 tccttctttt tctttgtttt aactcgtctg cccaacattt taaaacaagg ttttcaacca
     1441 gccttctcag ccctcacctt cccaaccatt atcacagcga cctcgctcaa gatggctcag
     1501 ggaattttga aacttccatt tctggattac ctggtattgg ctgaaaccat tatatgccta
     1561 actattttat tctttgtact aggtgcttat ctgatttggt tacgaaaaaa ggtctagcta
     1621 gaaatagcta aaccttattt tttatggttt gatgacttca gctccaccca tgtatggacg
     1681 aagtgcttct gggatggtca cagaaccatc ttcattttgg taattttcaa gaattgcagc
     1741 cactgtacgt ccaactgcaa gtccagaacc gttcaaggta tgaaggagtt tcaccttgcc
     1801 atctgcttca tcacggtaac ggatttgggc acgacgggct tggaaatctt ctgtgtttga
     1861 acagcttgag atttcacggt aattgttttg tgctggaatc cacacttcca agtcgtaagt
     1921 cttcgtagct gagaagccca tatctccagt agagagagca acgacacggt atggaaggtt
     1981 gagtttttga agaatgtttt cagcgttggc tgtcattttt tccaattctt cgtaagattc
     2041 ttctggtttg gcaaatttga ccatttcaac cttgtggaat tggtgcaaac ggatcaagcc
     2101 acgcgtatca cgaccggcag aaccagcctc agaacggaat gacggactca tagcagtgaa
     2161 gtagattgga agatctttgc cgtctaagat ttcatcacgg tagtagtttg tcagaggaac
     2221 ttcagcagtt ggaatcaaga caaagttggt atcgctgagt tcaaaagtat cttccttaaa
     2281 ttttggatac tgaccagtac caaacataga atcatggttg actatgtaag gtgtgatgac
     2341 ttcagtatag ccttcttttc catgttcatc caacataaag ttgtagatag cacgttccaa
     2401 acgagcaccg aggcctttat aaaagaggaa gcgagcgcct gttaccttac caccgcgttc
     2461 ccagtcaagg ataccaaggt cttcaccgag atcccagtga gctttaggtt cgaagtcaaa
     2521 ctcgcgtgga gtaccccaac ggcgaacttc cacattgtcg tcttcgtcag ccccaacagg
     2581 aacgctgtca gctgggatat ttggaagagt cgttgtaaat tctgtcaatt tagcatcgat
     2641 ttctgccaat tcagcatcca aggctttaac ctcagcagat agattttgca tggcagcaat
     2701 cttgtcatct gtattttcct tgttgcgctt agcttgggca atctcagcag aaactgtgtt
     2761 acgttctgct ttgagagttt caaccttgac caagatgtta cgacgtttag catcgatttc
     2821 tttcatttca ttcaagacag cagcatctac accacgtgta gctaattttt ctgcgacagc
     2881 ttcaaaatct gtacgaatac gtttgatatc taacataaga actcctttat gaaaaaagca
     2941 cgcctgacaa aacgttggag tggcagggcc acggttccat ccaacttcac aggtgtgcac
     3001 ttgattgtgt atgtaattgt tactaacggt agaatttcac ctatccctcc tatctgctcg
     3061 cagtacccgc agactttctg aaagaagaag ataacctact tatccgttgc tatgattata
     3121 ctaaagtttc tacttttttg caaatagatt tttaaatttt tggctaattg tctgaatcag
     3181 ggtcggaagt ttgacgacct tgtcattgcc tagtttttcg cgtgcaattt tgagaatggc
     3241 acctgagtct tttgaagcaa agaggaattt tcctttgtct gtaaagactt cgaagtggcg
     3301 gctgattttg catccagtga cattggctcc aatctgattg atatggctcc aaggaatctg
     3361 gataaattgt tcgacattga catctgggta aaattccaaa gcctgatctc cgacaaggaa
     3421 tttcccaact ttcccagcga tagagaggta ggaagtgcct gtcgtactga ggagtactgt
     3481 tttgttaagt gattgagcca tgcttagtct tccttacttt ctccaaaaaa ggcattgtag
     3541 agggctttaa ttgctgcttt ctcttggtct ttattgacaa caaacataat agaaacttca
     3601 ctagaacctt gagacatcat ctggatgttg attttgtttt cagatagagc gcgtgtcgca
     3661 gtagcagtca ctccgatgtg gctcttcatt ttttcaccaa caatcataat gatagaaagg
     3721 tcgtgttcga tttctgcatg atctacttta gccttttgaa ccaactgacg caggatttct
     3781 tcttccttga tgggagttag ttggcgagaa cggagaatga tagaaagatc gtcgatacct
     3841 gttggcatat gttcccaacc gatgttaagt tcttccagga tttgcagaac cttgcggcca
     3901 aatccaacct cacggttcat gaggtatttc gacatgttaa tactgacaaa gcctgagtca
     3961 ccagcaattc ccacaactgg aaattcatca ttactgtgtt ttagaacgat acgagtacct
     4021 ggatggtcag ggttgttggt attcttgata accagaggaa tttttccacg gtaggcagga
     4081 agaagagcct cgtcatgaag gactgagaag cctgcatagg ccaactcgcg catttcacgg
     4141 taggtcaact caggaatcga gtgtggttgg tggataatac cagggtgggc tgcaaagata
     4201 ccatcaacgt ccgtaaagtt ttcatagagg tcggctttga caccagcagc aatgatagaa
     4261 cctgtaatat cagaacctcc acgtgagaaa gtacagattt gattttcctt agtgacacca
     4321 aagaaaccag gaatgacaag gacttcattg gtgtttgtca attcttcaat cttgtcataa
     4381 cttgatggaa tgatgcgagc gtgaccaggt tcacttgtga ccacaatccc agcttctcta
     4441 gggtgcatat agcgtgcatc gataccattt tggttaaagt aggcagcaat caatttggca
     4501 ttgttatttt caccggctgc taggaaagta tcgtagagaa attcattttc ttcaatagga
     4561 agagtggcca aggcgtgaat gcttttagaa attttttcta gcacagctgg ttttagtccc
     4621 aattcactaa ccatagcagc atagcggtcg ataatccagc tttggctctt gctaatatcg
     4681 ttacccgcaa catagtcgcg gtagtattta atcagggcat ccgtaacctt agtatcttca
     4741 gcattgcgtt taccaggcgc agaaacgact acaaaacgac gctctgaatc gcttttgacg
     4801 atgtttaaaa ctttttctaa ttgactagca gaggcaagag aactacctcc aaatttaaca
     4861 actttcataa gaactcctaa agtaagtatt ttatacgatt atagcagaaa gaaaggcttt
     4921 tttcaatgaa gaaaataaga tgcgttctag cctaaagcga gcctttctta tcggctgaga
     4981 cactttcagt cgattttttc ttgctttctg ctcctaaaaa agatatactt tgacagtgaa
     5041 ataatttaac actcaatgaa aatcaaagag caaactagga agctagccgc agactgctca
     5101 aagcactgct ttgaggttgt aaataagact gacgaagtca gctcaaaata ctgttttgag
     5161 gttgcagatg gaagctgacg cggtttgaag agattttcga agagtataaa ctgcttataa
     5221 aataaaaagg agccctgatg gaacacatta tttatcagct tgaagaggac ttggcaatcc
     5281 ttaccttgaa ccgtcctgag gtcgcaaatg gttttcatat tcccatgtgt gaggagattt
     5341 tagaagctct gactttggca gaagaaaatc cagctgtgca ttttatctta atcaatgcca
     5401 atggaaaggt cttctcagtt gggggagatt tggtagagat gaagcgggca gtggatgagg
     5461 atgatattcc atcattgaca aaaatcgcag agttggtcaa tacgatttct tataaaatca
     5521 agcaaatagc caaacctgtt ttaatggaag ttgacggtgc tgttgcaggt gccgcagcga
     5581 atatggctgt tgcggcagat ttctgtttgg cgacggataa ggctaagttt atccaagcct
     5641 ttgttggtgt tggtttggct ccagatgcag ggggaattca tctcttgagc cgcagtatcg
     5701 gtgtgacgcg tgctgctcaa ttagctatga caggagaggc tttaacggca gaaaaagccc
     5761 tagaatgggg gttggtttac cgcgtttctg aagctgaaaa acttgaaaag acgagagaac
     5821 agcttcttaa aaaattaaga cgtgcttcaa gtaattccta tgctgccatt aagaagttgg
     5881 tttgggagag ccaatttaaa gactggcagg gttacgctac tttagaactg aacctacaga
     5941 aatccttagc tcaaacagag gatttcaaag aaggagtgcg agctcattcg gagagaagaa
     6001 gacctaaatt tacaggaaaa taaaaaatac ttgcatcatt ctttgaattt tgatataatt
     6061 ctttctgtca aatgttttga ttgtaaaagt tttttgaaga aggagggata tgaaaaatgg
     6121 actaccaacg aatcaatgaa tatttaacat ctatatttaa caatgtcctt gtaattgagg
     6181 aagtgaactt gagaggtagt cgttttaagg atatctccat caaagaaatg catacgattg
     6241 atgtcatcgg taaggctcca gatgtgactc caagtcaagt gtcaaaagag ttgatggtaa
     6301 ctcttggaac tgttacgaca agtttgaaca atttagagcg taagggctac attgagcgag
     6361 ttcggtcaga acaggatcgt cgtgtggtgc atctgcattt gacaaagaag ggtcgcttga
     6421 ttcatagact gcataaacgc ttccacaagg ccatggtaga aaaaattatt gatggcatga
     6481 gcgaggaaga aattgctgtc atgggtaaag gcttgactaa tctttaccaa tttttggagg
     6541 atttgaaata atggcttttg caaaaataag tcaggttgct cattatgtgc cagagcaagt
     6601 ggttacaaat cacgacttgg ctcagattat ggataccaat gatgagtgga tttcaagtcg
     6661 aacgggaata cgacaaaggc atatttcaag aacagaatct accagtgatt tggctacaga
     6721 ggttgctaag aaactgatgg caaaagctgg aataacagga aaagaactgg attttatcat
     6781 cctagctacc attactccag attcgatgat gccctctaca gctgctcgtg ttcaagctaa
     6841 tattggcgct aataaagcct ttgcttttga cttaaccgcg gcttgcagtg gatttgtatt
     6901 tgctctttca actgctgaaa agtttatcgc ttctggtcgc tttcaaaaag gcttggtgat
     6961 tggtagtgaa accctctcta aggcagtcga ttggtcggat cgatcaacag ctgtgttgtt
     7021 tggagatggt gctggtggtg tcttgttaga agctagcgag caagagcatt tcttagctga
     7081 gagtctcaat agcgatggaa gtcgcagcga gtgtttaact tatgggcatt caggtttgca
     7141 ttctccattt tcagatcaag aaagtgcaga ttcgtttttg aagatggatg gacgcacagt
     7201 ctttgatttt gccattcgag atgtagccaa gtctatcaag cagactattg atgaatctcc
     7261 tatagaggtg acagacttgg attatctgct acttcatcaa gccaatgacc gtattttgga
     7321 taagatggct agaaaaattg gtgttgaccg agccaaactt ccagccaata tgatggaata
     7381 tggcaatacc agtgcagcca gtatcccgat tttactttca gagtgtgtag aacaaggtct
     7441 catcccttta gatggtagcc agactgttct tctatcaggc ttcggtggag gcttgacctg
     7501 gggcacgctc attcttacaa tttaggtaat catgtggtga acacattgtt ataaaaaaac
     7561 tatttatttt aaaggagtcc tatcatggca gtatttgaaa aagtacaaga aattatcgtt
     7621 gaagaacttg gaaaagacgc atcagaagta acacttgaat caacttttga tgatttggac
     7681 gcagattcat tggacttgtt ccaagtaatc tcagaaatcg aagatgcttt tgatatccaa
     7741 atcgaagcag aaaatgactt gaaaacagtt ggtgacttgg ttgcttacgt tgaagagcaa
     7801 gcaaaataag cagaatatta gtagaaggag tagggagaaa cccactccct cttgtttagg
     7861 taatagtttg actgtcaaat tatggtgaaa tcgaactatt acgtaagcaa ggaaggttgg
     7921 aggcactatg aaaacgcgta ttacagaatt attgaagatt gactatccta ttttccaagg
     7981 agggatggcc tgggttgctg atggtgattt ggcaggggct gtttccaagg ctggaggatt
     8041 aggaattatc ggtgggggaa atgccccgaa agaagttgtc aaggccaata ttgataaaat
     8101 caaatcattg actgataaac cctttggggt caacatcatg ctcttatctc cctttgtgga
     8161 agatatcgtg gatctcgtta ttgaagaagg tgttaaagtt gtcacaacag gagcaggaaa
     8221 tccaagcaag tatatggaac gtttccatga agctgggata atcgttattc ctgttgttcc
     8281 tagtgtcgct ttagctaaac gcatggaaaa aatcggtgca gacgctgtta ttgcagaagg
     8341 aatggaagct ggggggcata tcggtaaatt aacaaccatg accttggtgc gacaggtagc
     8401 cacagctata tctattcctg ttattgctgc aggaggaatt gcggatggtg aaggtgctgc
     8461 ggctggcttt atgctaggtg cagaggctgt acaggtgggg acacggtttg tagttgcaaa
     8521 agagtcgaat gcccatccaa actacaagga gaaaatttta aaagcaaggg atattgatac
     8581 tacgatttca gctcagcact ttggtcatgc tgttcgtgct attaaaaatc agttgactag
     8641 agattttgaa ctggctgaaa aagatgcctt taagcaggaa gatcctgatt tagaaatctt
     8701 tgaacaaatg ggagcaggtg ccctagccaa agcagttgtt cacggtgatg tggatggtgg
     8761 ctctgtcatg gcaggtcaaa tcgcagggct tgtttctaaa gaagaaacag ctgaagaaat
     8821 cctaaaagat ttgtattacg gagccgctaa gaaaattcaa gaagaagcct ctcgctgggc
     8881 aggagttgta agaaatgact aaaacagcct ttttatttgc tggtcaaggt gcccagtatc
     8941 tagggatggg acgggatttc tatgatcagt atccgattgt caaagaaacg attgatcgag
     9001 cgagtcaggt gctcggttat gatttacgtt atctcatcga tacggaagaa gacaaactca
     9061 atcagacccg ctatacgcaa ccagccattc tagcgacttc ggttgctatc taccgtttat
     9121 tgcaagaaaa gggctatcag cctgatatgg ttgctggttt gtctcttgga gaatactctg
     9181 ccttggtggc aagcggcgcc ttggattttg aagatgcggt tgccttggta gctaagcgtg
     9241 gagcctatat ggaagaagcg gctcctgctg actctggcaa gatggtagca gttctcaata
     9301 cgccagtaga ggtcattgaa gaagcctgtc aaaaagcttc tgaacttgga gtggttactc
     9361 cagccaacta taacacacct gcacaaatcg tcattgctgg agaagtggtt gcagttgatc
     9421 gagcggttga acttttgcaa gaagcaggtg ccaaacgctt gattcctctt aaggtgtcag
     9481 gtccctttca caccgctctc cttgagcctg ctagccagaa actagctgaa actctagctc
     9541 aggtaagttt ttcaaatttt acttgtcccc tagtcggcaa tacagaagct gctgtgatgc
     9601 aaaaagagga cattgctcag ctcttgacgc gtcaggtcaa ggaacccgtt cgtttctatg
     9661 aaagtattgg ggtcatgcaa gaagcaggca taagcaactt tatcgagatt ggaccgggga
     9721 aagtcttgtc aggttttgtt aaaaaaattg atcaaactgc tcacttagct catgtggaag
     9781 atcaagcgag tttagtagca cttttagaaa aatagactaa aataagtaga agttttgaaa
     9841 ggaaaaaaat gaaactagaa cataaaaata tctttattac aggttcgagt cgtggaattg
     9901 gtcttgccat cgcccacaag tttgctcaag caggagccaa cattgtctta aacagtcgtg
     9961 gggcaatctc agaagaattg ctcgctgagt tttcaaacta tggtatcaag gtggttccca
    10021 tttcaggaga tgtatcagat tttgcagacg ctaagcgtat gattgatcaa gctattgcag
    10081 aactgggttc agtagatgtt ttggtcaaca atgcagggat tacccaagat actcttatgc
    10141 tcaagatgac agaagcagat tttgaaaaag tgctcaaggt caatctgact ggtgccttta
    10201 atatgacaca atcagtcttg aaaccgatga tgaaagccag agaaggtgct atcattaata
    10261 tgtctagtgt tgttggtttg atggggaata ttggtcaagc taactatgct gcttctaagg
    10321 ctggcttgat tggctttacc aagtctgtgg cacgcgaggt cgctagtcgg aatatacgag
    10381 tcaatgtgat tgctccagga atgattgagt ctgatatgac agctatctta tcagataaga
    10441 ttaaggaagc tacactagct cagattccga tgaaagaatt tgggcaggca gagcaggttg
    10501 cagatttgac agtattttta gcaggccaag attatctaac tggtcaagtg gttgccattg
    10561 atggtggctt aagtatgtag cgaaagctag aggtgaaaag aatgaaacta aatcgagtag
    10621 tggtaacagg ttatggagta acatctccaa tcggaaatac accagaagaa ttttggaata
    10681 gtttagcaac tgggaaaatc ggcattggtg gcattacaaa atttgatcat agtgactttg
    10741 atgtgcataa tgcggcagaa atccaagatt ttccgttcga taaatacttt gtaaaaaaag
    10801 ataccaaccg ttttgataac tattctttat atgccttgta tgcagcccaa gaggctgtaa
    10861 atcatgccaa tcttgatgta gaggctctta atagggatcg ttttggtgtt atcgttgcat
    10921 ctggtattgg tggaatcaag gaaattgaag atcaggtact tcgccttcat gaaaaaggac
    10981 ccaaacgtgt caaaccaatg actcttccaa aagctttacc aaatatggct tctgggaatg
    11041 tagccatgcg ttttggtgca aacggtgttt gtaaatctat caatactgcc tgctcttcat
    11101 caaatgatgc gattggggat gccttccgct ccattaagtt tggtttccaa gatgtgatgt
    11161 tggtgggagg aacagaagct tctatcacac cttttgccat cgctggtttc caagccttaa
    11221 cagctctctc tactacagag gatccaactc gtgcttcgat cccatttgat aaggatcgca
    11281 atgggtttgt tatgggtgaa ggttcaggga tgttggttct agaaagtctt gaacacgctg
    11341 aaaaacgtgg agctactatc ctggctgaag tggttggtta cggaaatact tgtgatgcct
    11401 accacatgac ttctccacat ccagaaggtc agggagctat caaggccatc aaactagcct
    11461 tggaagaagc tgagatttct ccagagcaag tagcctatgt caatgctcac ggaacgtcaa
    11521 ctcctgccaa tgaaaaagga gaaagtggag ctatcgtagc tgttcttggt aaggaagtac
    11581 ctgtatcatc aaccaagtct tttacaggac atttgctggg ggctgcgggt gcagtagaag
    11641 ctatcgtcac catcgaagct atgcgtcata actttgtacc aatgacagct gggacaagtg
    11701 aagtatcaga ttatatcgaa gttaatgtcg tttatggaca aggcttggag aaagaaattc
    11761 catacgctat ttcaaatact tttggttttg gaggccacaa tgcagttctt gctttcaaac
    11821 gttgggagaa tagataagta tgaatttaaa cgatattaaa gacttgatga ctcaatttga
    11881 ccagtcaagt ttgagagaat tttcttataa aaatgggacg gatgagttgc agtttagcaa
    11941 gaatgaagcg agacctgtgc ctgaagttgc aactcaagtc gctccagcac ccgttctagc
    12001 aacaccgagt ccagtagctc ctacatctgc tccagcagag actgtagcag aagaagttcc
    12061 agctccagca gaagcaagtg tggctactga gggaaatctt gtagagagtc cacttgttgg
    12121 agtggtttac ttggctgctg gaccagataa acctgccttc gttacagttg gtgatagtgt
    12181 caaaaaaggt caaacattgg taattatcga agccatgaaa gtcatgaatg aaatcccagc
    12241 tcctaaggat ggtgtggtaa cggaaattct cgtctctaac gaggaaatgg ttgagtttgg
    12301 caaaggattg gtacgtatca aatgatcgat attcaaggaa tcaaagaagc ccttccccac
    12361 cgttatccta tgcttctagt ggaccgtgtc ttggaagtga gcgagaatac cattgttgct
    12421 atcaaaaatg tgaccatcaa cgagcctttc tttaacggcc actttcctca atacccagtt
    12481 atgccaggtg ttctgattat ggaagccttg gcgcaaactg ctggtgtgtt ggagttatca
    12541 aaacctgaaa ataaaggaaa actggtcttt tacgctggta tggacaaggt taagttcaag
    12601 aagcaagttg taccaggcga ccaattggtt atgacagcga cttttgtaaa acgtcgtggc
    12661 accatagctg tggttgaagc aaaggctgaa gtggatggca agcttgcagc cagtggtatc
    12721 cttacttttg caattgggaa ctaaggaggt tctccatgtt tcgaaaaatt ttaattgcca
    12781 atcgtggtga aattgcggtt cgtattatcc gtgcggcacg tgaattgggg attgcgacgg
    12841 tagcggttta ttcaactgct gataaggaag ctcttcatac gcttttggca gatgaagcag
    12901 tttgtattgg tcctggcaag gcaacagagt cttatctcaa tattaatgca gttctatcag
    12961 ctgcagtctt gactgaggca gaagctattc accctggttt tggatttctc agtgaaaatt
    13021 ccaaatttgc gaccatgtgt gaagaagtag gtatcaagtt tatcggtcca tctggtcatg
    13081 ttatggatat gatgggggat aagatcaatg cgcgtgctca gatgattaaa gcaggtgtgc
    13141 ctgttatacc aggttcagat ggagaagtgc ataactctga agaagctttg attgttgctg
    13201 aaaaaattgg ctatcctgtt atgctcaagg cttcagcagg tggaggtggt aaagggattc
    13261 gtaaggttga aaaaccagat gacctcgttt ctgcctttga aactgcctct agtgaggcca
    13321 aggccaatta tggcaatggt gccatgtaca tagaacgggt tatctatcca gctcggcaca
    13381 ttgaggttca aatcctaggt gatgagcatg gacatgtgat tcacttgggt gaacgggatt
    13441 gttctcttca aaggaataac caaaaggttt tggaagaaag tccctcgatt gcaatcggaa
    13501 aaacgctgcg tcatgaaata ggtgctgctg ctgttcgagc ggcagagttt gttggctatg
    13561 agaatgcagg aaccattgaa tttcttcttg atgaagcaag tagcaatttc tatttcatgg
    13621 agatgaatac tcgtgttcag gtagaacatc cagtaacaga gtttgtttca ggtgttgata
    13681 tcgttaagga acagatttgc attgcggcag gtcagccttt gtctgttaag caagaagata
    13741 ttgtcctacg cggtcatgcc atcgagtgtc gtatcaatgc agaaaaccca gcctttaact
    13801 ttgctccaag tccaggtaag attactaatc tctatctgcc aagtggtgga gttggcttgc
    13861 gcgtggattc agcagtttat ccaggttata ccattccgcc ttattatgat agtatgattg
    13921 ccaaaatcat agtacacggc gaaaatcgtt ttgacgcctt gatgaaaatg caacgtgccc
    13981 tctatgaatt agagattgaa ggagtgcaga ccaatgcaga tttccagctt gacctcattt
    14041 cagatcgcaa tgtcattgct ggggattatg atacttcctt cttgatggaa accttcttac
    14101 ctaaatatca agaaaaagaa taaaatgact ttaagagttt aaaccgaaaa ggggaatcaa
    14161 tggctctatt tagtaaaaaa gataagtata ttcgaatcaa tcccaatcgt tcagttaggg
    14221 aaaaacctca agctaagcca gaggttccag atgaattatt ttcccagtgt ccaggctgta
    14281 agcataccat ctatcagaag gatctgggaa gtgaacgtat ctgtccgcac tgtagctata
    14341 cctttcgtat ttctgcccaa gaacgcttgg ctttgacgat tgatatggga accttcaaag
    14401 aattgtttac agggattgaa agcaaggatc ccttgcattt ccctggttac caaaagaaac
    14461 tggcatctat gcgtgaaaaa acaggtctgc atgaagccgt tgtgacagga actgctctta
    14521 ttaaaggtca gactgtggct cttgggatta tggattctaa ctttatcatg gcttctatgg
    14581 gtacggttgt aggtgaaaaa atcactcgtt tgtttgagta tgcgactgtc gaaaaattgc
    14641 cagttgtcct attcacagcc tctggtggag cccgtatgca ggaaggaatc atgagtctca
    14701 tgcagatggc taagatctct gcggcggtta aacgtcattc aaatgctggt ctcttttacc
    14761 tgaccatttt gacagatcca acgactggtg gtgtgacagc ttctttcgct atggaaggcg
    14821 atatcattct ggctgaacca cagagcttgg ttggttttgc tgggcgtcgt gtgattgaaa
    14881 atacggttcg tgaaagcttg cctgaggatt tccaaaaggc agaattccta ttagaacatg
    14941 gctttgtgga tgctattgtc aaaagaagag acttaccaga tacgattgct agcctagtca
    15001 gattgcatgg agggagtcct agatgaatat tgcaaaaata gtcagagaag cgcgtgagca
    15061 gagtcgcttg acaaccttgg attttgcgac aggcattttt gatgaattta tccaattaca
    15121 tggtgaccgt tcttttcgtg atgatggtgc agttgttggt ggtattggtt ggcttggaga
    15181 tcaagctgta acagtggttg gtatccaaaa aggcaagagt ttgcaagaca acctcaaacg
    15241 gaattttggc caaccacatc cagaaggcta ccgaaaggca ctgcggttga tgaaacaggc
    15301 tgagaaattt ggccgtccag ttgtgacctt tatcaataca gcaggtgctt atcctggtgt
    15361 cggagcggaa gaacgtggtc aaggggaagc tatcgctcgc aatctcatgg aaatgagtga
    15421 cctgaaagtt cctattatcg ccattattat cggtgaaggt ggttcaggcg gggctctggc
    15481 tctagctgtc gcggaccgtg tctggatgct ggaaaattct atctatgcca ttctcagtcc
    15541 agaaggcttt gcttccattt tatggaagga cggtactcgc gctatggaag cagcagaact
    15601 gatgaaaatc acttcgcatg aactgttaga aatggacgtg gtggataagg tgatttctga
    15661 aataggactt tctagtaaag aactgattaa gagtgtcaaa aaagaactcc aaacggagct
    15721 ggctagactt tcacaaaaac cgctagaaga gttgctggaa gaacgctatc aacgatttag
    15781 aaaatactaa gaatgactaa atgagactag aaaggtctca ttttttatgg aaaaatgtaa
    15841 ttcaggtcga aaaattgcca gttgaggaga tgggctataa aatagacaaa aaaagaaata
    15901 tactataaat gtgagcctac aacaatctat tttaagaagg tacttgcaat gacaggtaca
    15961 aatacattta cagttctttc aactgaggac ttggagcaaa cttcaggtgg tcttgctgtt
    16021 tgggaagatg gatatagtag atggttatat tatagagaat ttgctcccta tatgaggcaa
    16081 ggggcactta attcttatat agatgcttgg aagtacggct tccgagcagg gtaatctttc
    16141 aaaaaagtac gatgagaaac acaaaaaaac taagacaatt tggaatattt ttgcttatta
    16201 ttttactatc tacctattta ccacaaacaa ttggattgta tgtgacgatc attttagggt
    16261 taggagctga tgtctattct cttattttaa caatgggatt agtgggaagt ttccttctat
    16321 taatttggag gttaaaaaag aaaaagatgc tctttatctt tgagaaaaag agctggaact
    16381 ggtcatttgt cttctactta tttgcaactt atgtggttta tcagattctc ggtaattttt
    16441 gggcacgcta tgctcatttg attaatcata ggaatattca tgatgagtat tttaccgtgc
    16501 ttttttcaaa tggacaacca acctttttat cgactatctt atcttttgtc ctgcctgtaa
    16561 tcatcggtcc tgtttttgag gagactcttg atagagggta ttttatgaat accttctttc
    16621 cctagtccaa gtactatttg gatgttatcc tatctgctat catctttggg attagccatt
    16681 tgatactatc tcacagagat ccaattagtt tattatatta tagtctgatt ggtttctttt
    16741 ttgcccttgt ttatcgttct accgacaatc tgagattaac gattctctgc catagttttt
    16801 ttaactttct caatcatgca aaacctatct ggatttttgt ttacaattat atctattatc
    16861 atttttttag atagtgggag ccagaaaaag tgtttgatag atatcaaaca ctttttcttt
    16921 ttattgttct tctgttacaa actggctgag cagtccattg ataaaacggg cagatttttg
    16981 atcggagaag tcctttgcaa gctcgatagc ttcattaaca gcaaccagct gaggagtgtc
    17041 aaatgaagtg atttcaaaga ctcccaagcg aaggaggttt ctctccacga gcgttaagcg
    17101 ttcaatggtc caacctgctt ttaaatgctg agtgatttgc ttatctagtt cttccttttt
    17161 agcttgaaca ccagaaacga ggtctatcaa aaaggctgga agttgtacat ccgtatcttc
    17221 acgatcatga gtataggcga aacgacaagc agtttcgaca tccgtaccga actcaaggct
    17281 catgagagct tgaaaagcgc atttacggag ttggcgtcta gattctaata gtggactagt
    17341 cattgaggaa gtcctcgtca aatagatctt tcaattctgg ttttggtgtt ttatctggga
    17401 cgatacctgc aacgtgaata ttgatagcag cgagttctac atcagccata ttacggacgg
    17461 catctttgac agctttctgg atagcaaccg ctaccttagg aacttttact ccgtactcaa
    17521 ggtagagata gatatctgct gtgagttctt cgtccacgtt tttaagataa atgccacggc
    17581 cgagtgaaag ttttgaaagg gtatcagaca ctgatctgtt tgaaaaagag tgaaccccct
    17641 ctacctttgc agtagcgata gcaatgattt tttcaagtac acgtggggcg ataacgattt
    17701 cgccaagttg ttcttcaatt cccatagaat gacctctttc tagagattag gcacgagaaa
    17761 cgtaagttcc ttctgcagtg ttgataacga gtttttgtcc tgcttcgatg aagtctggaa
    17821 cgtttacgac aagtccagtt tccatcgttg ctggtttacc agaacctgta acagtagcac
    17881 ctttgataga tggttgagtt tcagcaactg tcaactcaac agtagtagga acggtgacac
    17941 cgatcacttc agttccgtag aattggattt tcacatcaga gttttcaagg atgtaaagca
    18001 attcgttttc aacattgact acagggattt cgtattggtc gtaagtttct gtattcatga
    18061 agtatgctgt gtcatccatt ttgtacaagt attgagctgg gacagtctcg ataatagctt
    18121 gttcaaattt ttcctctgga cggtagcttg tgtcaaatgt agaaccagta cggacatcac
    18181 gcaatttcat acgcatgatc gtgtttcctt tacctggttt gtggtgacta gcttccaaaa
    18241 cgcgaatcaa tttgccgtca gctgtttcaa aggtcatacc agcttttaat ttacttgctt
    18301 caatcatgtt tttacctctt taataaatat tattactctt cattttacca taaaatactc
    18361 tggaaataaa gccaaaatag ttattgggag ttggtagaga agaaaaaacc aaccttaggg
    18421 ctggttttgt tctgtctagt tttctttcaa cttcgccaat tcctgtgcaa gtagtttaag
    18481 ggcaacttgt gggttggctt ggcctttggt tgccttcata aggaatcctg taaaggcctt
    18541 gtcggcgtta cgtttgcctg acttgaagtc ggcaacagca gcttcgttat cggcaaagac
    18601 ttggtggatg attgggatca agatagctgg atctgaaatt tgaaccatac cagctttttc
    18661 cacgtattca cgcgcgccac cgccattttt agctagatgg acaaagactt tcttggcaat
    18721 cttagatgaa atagtaccgt cttcgatgat ggtaatcatt tcaaccaagt tttctggtgt
    18781 caattcgatt tgttccagtg ttttaccttc agcattcaag aactgagcga cttccccttg
    18841 gagccagtta gagacttgtt tggcatcacc acctagggca acagcttttt caaagaaatc
    18901 agaagtgact ttattagcag tcaactgact agcatcgtag tctgataaac caaggtcaga
    18961 tacataacgc gcacgacgtt cttttggaaa ctctggcaac tcagtccgca tttcctcaat
    19021 ccactcgtca gaaatttcaa agaggggtag gtctggttct gggaagtagc ggtagtcagc
    19081 agccccttcc ttgacacgca tgaggatggt tgctttattc gcttcatcgt aacggcgtgt
    19141 ttcttggcgg atttgaccac ctgagcgaag aatttcagcc tggcgttgga cttcgtattc
    19201 aagaccttta cgaacgtttg agaaggagtt gaggttcttc aattcagtct tggtaccgaa
    19261 tttctcttga ccataaggac gaagggagat gttggcatcc acacgcatcg aaccttcctc
    19321 catcttaacg tcagaaatgc cagcgtactg gataacttcc ttgagggctg tcagataagc
    19381 ataggcttct tcaggagaac gcatatctgc ctcagataca atctcaatca agggaacccc
    19441 ttggcggttg aggtcaacat aagagtagcc atctgtacca tgggtgtttt taccagcgtc
    19501 ttcctctagg tgggcacgtt cgataccgat tttcttggtc gtaccgtctt ctagttcgac
    19561 ttcaatccag ccgttataac cgattggctc atcaaactga gaaatttggt aggctttggg
    19621 attatcaggg tagaagtagt tcttgcggtc aaagtgcatc tttttgtgga tgtccatgtt
    19681 gagggcaaga gcagccttga ttccggcatc gacaacccct ttattgagaa ctggcagaac
    19741 tcctgggaaa gaccagtcaa tcacgttagt gttggcattt tgatcatttc cgaagtgggc
    19801 agaagtaggt gagaagattt ttgaattggt gttgagctct acgtggactt caagtccgat
    19861 gactgtttca aagttcatta gttgtcacct ccaaaaatca cgggttgttg tttgtggtag
    19921 cctgttgttg cttcaaaggc agcagcaact tggtaaatgg tttcctcaga gtgcttggga
    19981 ccaatcaatt gcagaccgac aggtagacct tgagagaatc cagcaggaat cgaaattcca
    20041 ggcagtcctg ccaagtttac aggtatggtc aataggtcgg ctaagtacat ggcaactggg
    20101 tcatggttga gagaatccaa gtcataggca acacttggag cagttggacc caaaatcaaa
    20161 tcgtaatccg cgaagacttt ttcgaaatct tgaatgataa gtgtacggac ctgtccagcc
    20221 ttcttatagt aggcatcgta gtaacctgat gaaagactga aagtacccag catgatacgg
    20281 cgcttcactt cttcaccaaa accttggctt cggctgttta catagatttc atcaaggttg
    20341 gttgcatctt ctgcgcgata gccgtaacgg ataccgtcaa agcgttgcaa gtttgatgaa
    20401 gcttctgatg aagcgatgat gtagtatacg gcaactccgt atttagagtg aggaaggctg
    20461 acttcttcga caatagcacc caatttttca aagtgtttag cggcatttag aatggtttcc
    20521 ttaacctctg ggttaattcc ttcaccgagg tattccttag gcaaagcgat tttcataccc
    20581 ttgatgtctt ggccgatttt tgaagtaaag tcggcgatgc ggacaggagc agaagtagag
    20641 tctttagcat cttcgctggc aatagcgttg agcaagaggg cattttcctt aacagtagga
    20701 gcaaaaggtc caatctggtc taatgagcta ccaaaggcaa tgagaccgaa acgtgaaact
    20761 gttccgtagg ttggtttgag accaacgatt ccgttgaagg cagcaggttg gcggatggaa
    20821 ccaccagtat cagaaccaag tgacaagcga acttgtcctg aggctacagc tgcggcagaa
    20881 ccacttgatg acccaccagg aaccttgctg tggtcccaag cgtttttagt tgctccgtag
    20941 tgtgaagttt cacctgaacc acccatagca aattcgtcca tgttggtctt tccaacgaca
    21001 atcatgccct tggtttttgc attggcaaca gctgtcgcat caaagattgg ctcatagttg
    21061 tagagcattt ttgaggcagc agttgtgaga ataccgtctg tagagatgtt atccttaaca
    21121 gcaagtggaa ttcctgaaag gacattgtca gcatcaattc cagcttcatc aatggcttta
    21181 gcttgaacaa gagcttgctc ctcagcgatg gtgacaaatg aattgagggc ttcctcacga
    21241 gacttgatat tttcaagtgt tgcttgggtc aattctgttg cagaaatttc cttagagaca
    21301 aggagattgt gcaactcttc aatagtttta ttgttaaaag tcattaggca tctcctccat
    21361 cgtctaggat agctggcacc ttgatatagt agttgtcttt ttcaggtacg tttttaaaca
    21421 agcgatcacg gtctgttcct tcttcggcca catcagggcg gagtacagtc ttgcggtcag
    21481 ccatagtcgt agtaggtgcg acaccagttg tgtcaacttc gcccagcaat tcaaccatgt
    21541 caacaatctt agacaaggtg gtcgcaaagg cagcagtttc tttttcagag aatcttaatt
    21601 ttgaaagatt ggcaacgtgt gttacctctt cttgcgtaat tttcatcttg catcctttcg
    21661 tgaaatgatg atttttagtc tgttctattt taccatattt tcctataaat aagggagggg
    21721 gaagtgaaaa gaaatagaaa ttgaaaaaaa tgctaaaatc cgatataatg gagtgttgaa
    21781 aggaatggta aaatccaatg taaaaatcat tctcagctat tgaaacaaga aaaatagaga
    21841 atcaaagaaa gagaacttat gaatattcaa gaagaaatta agaaacgtcg tacctttgcc
    21901 attatctccc acccggacgc ggggaaaaca accatcactg agcagttact ctactttggg
    21961 ggtgagattc gtgaggctgg tacggtaaaa gggaagaaaa cagggacttt tgctaaatct
    22021 gactggatgg atatcgagaa gcaacgtggg atttctgtta cttcatctgt tatgcaattt
    22081 gactacgacg gcaagcgcgt gaatatctta gacacgccag ggcacgagga cttctcagaa
    22141 gatacctatc gtaccttgat ggcggtggat gctgcggtca tggtcgtgga ctctgccaag
    22201 gggatcgagg ctcaaaccaa gaaattgttt gaggttgtga aacatcgtgg cattccagtc
    22261 tttaccttta tgaacaagct ggaccgtgac ggtcgtgagc ctttggatct cttacaagaa
    22321 ttggaagaaa tcttgggcat tgctagctac cctatgaact ggcctatcgg gatggggaaa
    22381 gcctttgagg gcttgtatga cctctataac caacgtttag agctttataa aggggatgag
    22441 cgttttgcca gtctagaaga tggggacaaa ctttttggta gcaatccttt ctacgagcaa
    22501 gtcaaggatg atattgagct tttaaatgaa gccgggaatg agttttcaga ggaagctatt
    22561 ctggctggag aattgacgcc tgtctttttc ggttcagccc tgacaaactt tggtgtgcag
    22621 accttccttg aaaccttcct caagtttgct ccagaaccac atggtcacaa gaaaacagac
    22681 ggtgaaattg tggatcctta tgacaaggat ttctcaggat ttgtctttaa aatccaagcc
    22741 aacatggacc ctcgtcaccg tgaccgtatt gcctttgtcc gtatcgtatc tggcgaattt
    22801 gagcgcggca tgagtgtcaa tctccctcgt actggtaagg gggctaaact gtctaatgtt
    22861 actcagttta tggcggaaag tcgtgagaat gtgatcaatg ccgtggcagg tgatattatc
    22921 ggggtttacg ataccggtac ttatcaagtt ggggatacct tgacagttgg taaaaacaag
    22981 tttgaatttg aaccactgcc aacctttact cctgaaattt tcatgaaagt ttctgctaag
    23041 aatgttatga agcaaaaatc cttccacaag gggattgagc aattggtgca agaaggagcc
    23101 gttcagcttt ataagaatta ccaaacaggt gagtacatgc taggcgctgt aggtcaactc
    23161 cagtttgaag tctttaaaca ccgcatggaa ggcgagtaca atgcagaagt agttatgaac
    23221 ccaatgggta aaaagaccgt tcgttggatc aagcctgagg acttggatga acggatgtcg
    23281 tcaagtcgca atatcttagc caaagaccgt tttgaccaac cagtctttct ctttgaaaat
    23341 gactttgccc tccgctggtt tgcggacaag tatccagacg tagagttaga ggagaagatg
    23401 tgactcagta ccaacaattg gaactaaagt tccaaattgt tggacgctag tcgctatttg
    23461 gcgaactagc taactgcctc actaactacg tttggcaaat agaatcgatt tgccaaactc
    23521 cgtgtcgtaa tgtaaaaatt gtaaaccgta atgggacttc atctaaaatc tccactggta
    23581 tggttttacg aacgaatagg cgaaaataat ggttacgtag aaacatatga tgggaataaa
    23641 agaaatgaga taaaaagtga aagaagagaa aaaagcgatt gtcttaggtg cagataatgc
    23701 ttatatggat aaagttgaaa caacaataaa atctctttgt gttcatcatt ataatcttaa
    23761 gttttatgtt tttaatgatg accttccgcg agaatggttc caattaatgg aaaaaagact
    23821 agagactttg aatagtgaga ttgtgaatgt ttagattgac agtagtattc tgaaaggtta
    23881 tcgtctcccc tttgagggac tttcatatgc ggctttttta ggtattttat tccaaagtat
    23941 gtaagcgagt ctcgtgtact gtatttggat tcagatattg ttgtaagaaa atcgatagat
    24001 gagctgtggg atttggactt gacggctata tccttagctg cagttagaga tgatttttat
    24061 acacataatt ttaattctgg tattctgtta atcaacaatg gcatgtggag agcagaaaat
    24121 gtcacgcaag acctgattta agacacaaat aaaaaatctt ccaattccct tgcaaaaaat
    24181 aagaaagtgt gataacatag tacggtatgt ggtgctagca catccgctat attagatcta
    24241 ataggaggaa aacacaatgg ctaaagtatg ttactttaca ggtcgtaaga ctgtatcagg
    24301 aaacaaccgt tcacacgcga tgaaccaaac aaaacgtgcc gtaaaaccaa accttcaaaa
    24361 agttactgtt cttatcgatg gtaaacctaa aaaagtttgg gcttcagctc gtgctttgaa
    24421 atcaggtaaa gttgaacgcg tttaataata aaagcaaagg agaccttagg gtcttttttc
    24481 ttttattgtg ggtataacat cgtttgaaaa atagagtaaa tatccgctga tacatcattc
    24541 tgcttttaca cttgggctga aatatgataa aatagagtat caactagttg aggtaaaaag
    24601 gatgactgta aaaattaata caaaagatgg tcaaatcgaa ctaacagatg aagtgattgc
    24661 aaccgtcgta ggtggagcag caactgagat ttttggtgtg gtcggtatgg ctagtaaaaa
    24721 tgccctcaaa gataatttcc aagccctgct aggtaaggaa aattattcta aaggtgtcgt
    24781 cgtaaaggca gccgaagatg gcagtattgc agttgatgta tataccgtgt taagctacgg
    24841 aacaaagatt agtgaagtgt caaaaaacat tcaagagcgt gttgccttta gcttggaaaa
    24901 ccagcttgga attactgctc agactgtaaa tgtctacatt caaaatatca aagttgtagg
    24961 agaataaccg tgtcaaaaat tactactagc ttatttcaag aaatggtgca ggctgcatca
    25021 actcgcttga ataagcaagc tgaatatgtc aattcattaa acgtctttcc agttccagat
    25081 ggagatactg ggacaaatat gggaatgacc attgaaaatg gtgctaaaga agttgcagac
    25141 aagccagctt ctacagttgg agaagtagcg agcattcttg ccaaagggct tttgatgggt
    25201 gcgcgtggga actcaggagt gattacgtct cagcttttcc gtggattttc ccaagctatc
    25261 aaggataaag acgagttaac aggtcaagac ttggctctgg ccttccaatc aggtgtggaa
    25321 gttgcctata aggcagtgat gaaacctgtt gaaggaacga ttttgacagt ttctcgtgga
    25381 gctgctatcg gtgctaagaa aaaagctgag caaacagatg acactgttga agtcatgcgc
    25441 gcagccttgg aaggtgctaa aacagctcta gctaaaacac cagacatgct tccagtattg
    25501 aaagaagttg gcgttgtgga ctcaggtggt caaggattgg tcttcatcta cgaaggtttc
    25561 ctttcagccc ttactggcga atatattgca tctgaggact ttgtagcgac tcctgccaac
    25621 atgagtgaga tgatcaatgt agagcatcat aagtctgtag ctggtcacgt agcgactgag
    25681 gacatcacgt ttggttactg tactgaaatc atggtagctc ttaagcaagg tccaacctat
    25741 gctaaagatt ttgactacga tgaattccgt aactacttgg atgagcttgg agattctctc
    25801 ctcgttgtta acgatgatga aattgtcaaa gtccatgttc atacagaaga tccaggactt
    25861 gttatgcaag aaggtctcaa atatggtagc ttggtcaagg taaaagttga caatatgcgt
    25921 aaccaacacg aagcacaggt tgagaaagaa gctactcaag ttatcaagtc ggctgaagaa
    25981 aaagagtatg ctttgattgc tgtggtggct ggtaaaggtc tagcagatat cttctgttct
    26041 caaggcgtgg attatgttat cgaaggcggt caaaccatga acccttcaac agaagacttt
    26101 atcaaggctg ttgaacaggt taatgcccgt aacatcatct tcttgccaaa caacaagaac
    26161 atcttcatgg cagctcaatc tgcggcagaa gttttggagc aaccagcggt agtggtagag
    26221 gctcgcactc ttcctcaagg tatgacaagt cttcttgcct ttgatccaag caagtccatt
    26281 gaagaaaacc aagagcgtat gacagctgct cttagcgatg tcgttagcgg aagcgtcaca
    26341 acagccgtgc gtgatacaac gatcgatggc ttagaaatcc atgaaaacga taatttaggt
    26401 atggtggatg gaaaaattct tgtgtcaaac cctgatatgc accaaacatt gactgaaacc
    26461 ttgaaacata tgttggatga agacagtgaa atcgtaacct tctatgtcgg tgaagacgga
    26521 agcgaagaac ttgccaatga aatcgctcaa gaaatcgtag aagaattcga agacgttgaa
    26581 gtcgagattc accaaggtca acaacctgtt tacccatacc tatttagtgt ggaataagat
    26641 taatgaagga ctgagaaatc agttcttttt tctttttagt aaatgaaatc ggtatctttt
    26701 taata
//
LOCUS       DAVMGZ010000013        26687 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000013 DAVMGZ010000000
VERSION     DAVMGZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.013"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(278..1198)
                     /gene="cysK"
                     /locus_tag="VYQ93_000466"
     CDS             complement(278..1198)
                     /gene="cysK"
                     /locus_tag="VYQ93_000466"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV3335210.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1483..2118)
                     /locus_tag="VYQ93_000467"
     CDS             complement(1483..2118)
                     /locus_tag="VYQ93_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV3335211.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLRDVEVPVNLV"
     gene            2174..3472
                     /locus_tag="VYQ93_000468"
     CDS             2174..3472
                     /locus_tag="VYQ93_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3335212.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3469..4131
                     /locus_tag="VYQ93_000469"
     CDS             3469..4131
                     /locus_tag="VYQ93_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV3335213.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            4211..4759
                     /gene="raiA"
                     /locus_tag="VYQ93_000470"
     CDS             4211..4759
                     /gene="raiA"
                     /locus_tag="VYQ93_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV3335214.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            4895..6868
                     /locus_tag="VYQ93_000471"
     CDS             4895..6868
                     /locus_tag="VYQ93_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV3335215.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDHVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            6865..7317
                     /gene="rplI"
                     /locus_tag="VYQ93_000472"
     CDS             6865..7317
                     /gene="rplI"
                     /locus_tag="VYQ93_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV3335216.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7361..8713
                     /gene="dnaB"
                     /locus_tag="VYQ93_000473"
     CDS             7361..8713
                     /gene="dnaB"
                     /locus_tag="VYQ93_000473"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV3335217.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8715..8981
                     /locus_tag="VYQ93_000474"
     CDS             8715..8981
                     /locus_tag="VYQ93_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335218.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            9070..10416
                     /gene="cbpD"
                     /locus_tag="VYQ93_000475"
     CDS             9070..10416
                     /gene="cbpD"
                     /locus_tag="VYQ93_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV3335219.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10499..10601
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10672..10962
                     /locus_tag="VYQ93_000476"
     CDS             10672..10962
                     /locus_tag="VYQ93_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV3335220.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            10925..11683
                     /locus_tag="VYQ93_000477"
     CDS             10925..11683
                     /locus_tag="VYQ93_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3335221.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            11721..12728
                     /locus_tag="VYQ93_000478"
     CDS             11721..12728
                     /locus_tag="VYQ93_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3335222.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            12728..13456
                     /locus_tag="VYQ93_000479"
     CDS             12728..13456
                     /locus_tag="VYQ93_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3335223.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVAEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13575..14033
                     /locus_tag="VYQ93_000480"
     CDS             13575..14033
                     /locus_tag="VYQ93_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV3335224.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            14035..16467
                     /locus_tag="VYQ93_000481"
     CDS             14035..16467
                     /locus_tag="VYQ93_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV3335225.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVFYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMSKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            17109..17762
                     /gene="rr06"
                     /locus_tag="VYQ93_000482"
     CDS             17109..17762
                     /gene="rr06"
                     /locus_tag="VYQ93_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV3335226.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            17759..19090
                     /gene="hk06"
                     /locus_tag="VYQ93_000483"
     CDS             17759..19090
                     /gene="hk06"
                     /locus_tag="VYQ93_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV3335227.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            19173..19700
                     /locus_tag="VYQ93_000484"
     CDS             19173..19700
                     /locus_tag="VYQ93_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV3335228.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            19776..21335
                     /gene="pspC"
                     /locus_tag="VYQ93_000485"
     CDS             19776..21335
                     /gene="pspC"
                     /locus_tag="VYQ93_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEV3335229.1"
                     /translation="MFASKNERKVHYSIRKFSIGVASVAVASLFLGGVAHAEGVRIGN
                     NSTVTSSGDEVESHLQSILKDVNKNLKKVQHTQNVGLLTKLSEIKRKYLYELKVNGLE
                     EKSKAELTSKTKKELTAAFEQFKKDTLSTELEKKVAEAQKKVAEAEKKAKAQKEEDHR
                     NYPTNTYKTLELEIAESDVEVKKAELELLKEEAKESRDEGTIKQAEAKVESKKAEATR
                     LEKIKTDRKKAEEAKRKAAEEDKVKEKPAPAPQPAPAPQPEKPAEETPAPAPKPEKPT
                     EQPKAEKPDDQQAEEDYARRSEEEYNRLTQQQPPKPEQPAPAPKIGWKQENGMWYFYN
                     TDGSMATGWLQNNGSWYYLNANGAMATGWLQNNGSWYYLNANGSMATGWLQYNGSWYY
                     LNSNGAMATGWLQNNGSWYYLNANGSMATGWLQYNGSWYYLNSNGAMVTGWLQNNGSW
                     YYLNANGSMATDWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDS
                     YRVNANGEWVN"
     gene            complement(21542..22120)
                     /locus_tag="VYQ93_000486"
                     /pseudo
     CDS             complement(21542..22120)
                     /locus_tag="VYQ93_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            22198..23007
                     /locus_tag="VYQ93_000487"
     CDS             22198..23007
                     /locus_tag="VYQ93_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV3335230.1"
                     /translation="MGWQEWLDQMLAEISYDENQQELIFFVGEADYQQELSKRGFGTV
                     LPERKFGISVTMIRENPSKYWKYIAQPFRRQFTKKVLIMGSASNGKTTLAKDLARYYD
                     APVSLEYAREYQIKNNVRDDELTPKDYYYLLLGQYDQTSKLIDSNANRGLVIADTNSL
                     VTKGYYDYYMETENQVDLSGETFDNLFVSILAKEKWDLILFVQPVGSYVNDGFRDMTM
                     AEEHIRYSFSQHLDQMRERYLTTIPLVYLAEDYLGNYEAAKVTIDAIYQAD"
     gene            23017..23743
                     /locus_tag="VYQ93_000488"
                     /pseudo
     CDS             23017..23743
                     /locus_tag="VYQ93_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            23752..24390
                     /locus_tag="VYQ93_000489"
     CDS             23752..24390
                     /locus_tag="VYQ93_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3335231.1"
                     /translation="MVAYCFVEGKIKLLLIRRKAHPYQNYLALVGGFMDKGEDAAHAY
                     QREVKEEVNLDLPLEKIEQLMTVSTPGRDPRGWTVTIAYLVYLPSRALELVQASDDAK
                     DVVFVDVDFQTGKCYLEGVELDEQALAFDHYAIIQKSIKRIQGRIDWNPTFLYLLEEE
                     FTVYEGTELVNFINPGRPIVSNNFLVKYGEYVEEVGLKRVPKKKPRKTYRLK"
     gene            24660..25306
                     /locus_tag="VYQ93_000490"
                     /pseudo
     CDS             24660..25306
                     /locus_tag="VYQ93_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730888.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25606..>26687
                     /gene="pspC"
                     /locus_tag="VYQ93_000491"
     CDS             25606..>26687
                     /gene="pspC"
                     /locus_tag="VYQ93_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HEV3335232.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLFMGSVVHATEKEGST
                     QAATSFNRGNGSQAEQRGELDSERDKARKEVEEYVKKIVGESYAKSTKKRHTNTVALV
                     NELNNIKNEYLNKIVESTSESQLQILMMESRSKVDEAVSKFESKFEKDSSSSSSSDSS
                     TKPETPQPENPEHQKPTTPAPDTKPSPQPEGKKPSVPDINQEKEKAKLAVATYMSKIL
                     DDIQKHRQIVALIKELDELKKQALSEIDNVNTKVEIENTVHKIFADMDAVVTKFKKGL
                     TQDTPKEPGNKKPSAPKPGMQPSPQPEVKPQLEKPKPEVKPQPEKPKPEVKPQPEKPK
                     PEVKPQPEKPKPEVKPQPEKPKPEVKP"
BASE COUNT         8440 a         4566 c         5969 g         7712 t
ORIGIN      
        1 gggaaagctg agaaacaagg gattacgctt taccatcatg tcctaatcga cttgttcata
       61 tcttatttca atccattata aatagtgaga aatatctatc ctatcttcta gaatgtcttc
      121 caaacgtgga aactctcgta aacaaagagg ttttagaggc ctatttacca tggactaaag
      181 ttgtacaaga aaagtgcaaa taagaaatct ccagattagg aactatccgt gagttctcta
      241 gtctggagat ttttcaatat acttcgttat tggacggtta caattcataa agtgctgtag
      301 agagataacg ttcaccgtta tctggtgcta gggcaaggac ttttttacct gtacctaatt
      361 ttttggcaac ctcgatggct ccgtagatag ctgcagctga ggaaatccct acaaggaagc
      421 cttcttttcc accaatttca cgtccgagtg caagagcgtc atctgatgtt acacgaacga
      481 taccatcata ggctttagta tcaagtgtat caggaataaa tccagctgag ataccttgaa
      541 ttttgtgagg accaggtttt tcaccagata gaatagcaga ttcatctgct tctactgcaa
      601 aaacttgaat gttagaattt tctgatttga gtgcatgaga aacaccagaa atcgttccac
      661 cggtacctac tccagcaaca aaggcatcta atccatcttt accgaaagca gctagtatct
      721 cagctcctgt tgttctttcg tgtacttctg gattagctgg attgtcaaat tgaagaggaa
      781 ggaaaccatc acgttcagca gcgatttctt gagccttagc aatagcacct ttcattccct
      841 cgctaccagg agttaggacg agttcagcac cataagcttg gataatttta cgtcgttcta
      901 cactcatagt ttcaggcata acgatgacga ctttataccc tttagcagca cctacccatg
      961 aaagtccaat accagtgttt ccacttgttg cttcaacaat agtagaacca ggtttcagaa
     1021 taccatcttg ttcagctttt tcaatcatgc taagggcaat acggtctttt acagatgaac
     1081 caggattaaa tgcttcaagc tttatataga cgtctgcagc accttctggc acgatgttgt
     1141 taagtttaac aatcggtgtt tgaccgatta attcagtaat gttgttataa atagacatat
     1201 gaatacctct ttgtataaga tatctttagt ataacgatat ctataccatt tgtaaaatat
     1261 agaaatccta tcaaagcgat aggatttttt tatatcatag atcaaagtca ttaattttca
     1321 agtgattgtg ttcagctaat tctagtaaat aggtgtgaag gaaaacgata tcaggtttcg
     1381 tttgaattgc ttcattttct agtatatttt ggtacgcatc tttttatttt gctcgctaaa
     1441 gtagttgtga gagtgtcaac gaaacgctgt attattcatt gtttacacta agtttacagg
     1501 aacttcaaca tctcgtaaac cttggtcagt taaagtgact tttccattaa aaaactccac
     1561 aagtgcagct ttaatagttt ctttttcttc tttatcaaca taaatcatcg tatcgacttg
     1621 atctgtaaag tttgtatcca gctccatgag atcatgttct ttaaggaagt tactgtactc
     1681 ttggtactga gcataagaca tttgaatagc aatgccagcc tgttctttta tttcaataat
     1741 accaatttct ttgacagcta aggcgacact gccggcgtaa gcacgaatta gtcctccagc
     1801 gcctagttta ataccaccaa agtagcgtgt cacgaccaca cagacattgg tgagattgtg
     1861 attttctagt accccaagca tgggaacacc agcagtacca ctaggctcac catcatcact
     1921 tgtacgttta atttcactac gttctccaat aataaaggca gagcagttat gtgtcgcttt
     1981 gtagtgttct tttttgatgg cagtaatgaa gtcacgagcc tcttcttcgc tataaacacg
     2041 cttggcatgg cagataaagc gagatttttt gatttcttct tggacttgac cgtcctcttt
     2101 aattgttcta aattccatga tttaattgta ctaaaaaatc atctaaagtg ctagttttta
     2161 cgaataaaga agtatgaaag taaatttaga ttatctcggt cgtttattta ctgagaatga
     2221 attaacagaa gaagaacgtc agttggcgga gaaacttcca gcaatgagaa aggagaaggg
     2281 gaaacttttc tgtcaacgct gtaatagtac tattctagaa gaatggtatt tgcccatcgg
     2341 tgcttactat tgtcgagagt gcttgctgat gaagcgagtc agaagtgatc aaactttata
     2401 ctattttccg caggaggatt ttccgaagca agatgttctc aaatggcgcg gccaattaac
     2461 tccttttcaa gagaaggtgt cagagggact gcttcaagca gtagacaagc aaaagccaac
     2521 cttagttcat gcggtaacag gagctggaaa gacagaaatg atttatcaag tagtggctaa
     2581 agtgatcaat gcgggtggtg cagtgtgttt ggctagtcct cgcatagatg tttgtttgga
     2641 gctgtacaag cgcctgcaac aggatttttc ttgcggtata gctttgctac atggagaatc
     2701 ggaaccttat tttcgaacac cactagttgt tgcaacaacc catcagttat tgaagtttta
     2761 tcaagctttt gatttgctga tagtggatga agtagatgct tttccttatg ttgataatcc
     2821 catgctttac cacgctgtca agaatagtgt aaaggagaat ggattgagaa tctttttaac
     2881 agcgacttcg accaatgagt tagataaaaa ggtccgttta ggagaactaa aaagactgaa
     2941 tttaccgaga cggtttcatg gaaatccgtt gattattcca aaaccaattt ggttatcgga
     3001 ttttaatcgc tacttagaca agaatcgttt gtcaccaaag ttaaagtcct atattgagaa
     3061 gcagagaaag acagcttatc cgttactcat ttttgcttca gaaattaaga aaggggagca
     3121 gttagcagaa atcttacagg agcaatttcc aaatgagaaa attggctttg tatcttctgt
     3181 aacagaggat cgattagagc aagtacaagc ttttcgagat ggagaactga caatacttat
     3241 cagtacgaca atcttggagc gtggagttac cttcccttgt gtggatgttt tcgtagtaga
     3301 ggccaatcat cgtttgttta ccaagtctag tttgattcag attggtggac gagttggacg
     3361 aagcatggat agaccgacag gagatttgct tttcttccat gatgggttaa atgcttcaat
     3421 caagaaggcg attaaggaaa ttcagatgat gaataaggag gctggtctat gaagtgcttg
     3481 ttatgtgggc agactatgaa gactgtttta acttttagta gtctcttact tctgaggaat
     3541 gatgactctt gtctttgttc agactgtgat tctacttttg aaagaattgg ggaagagaac
     3601 tgtccaaatt gtatgaaaac agagttgtca acaaagtgtc aagattgtca actttggtgt
     3661 aaagagggag ttgaagtcag tcatagagcg atttttactt acaatcaagc tatgaaggat
     3721 tttttcagtc ggtataagtt tgatggagac ttcctgttaa gaaaagtttt cgcttcattt
     3781 ttaagtgagg agttgaaaaa gtacaaagag tatcaatttg ttgtaattcc cctaagtcct
     3841 gatagatatg ctaatagagg atttaatcag gttgagggct tggtagaggc agcaggcttt
     3901 gagtatctgg atttattaga gaaaagagaa gagagagcca gttcttctaa aaatcgttca
     3961 gagcgcttgg ggacagaact tcctttcttt attaaaagtg gagtcactat tcctaaaaaa
     4021 atcctactta tagatgatat ctatactaca ggagcaacta taaatcgtgt taagaaactg
     4081 ttggaagaag ctggtgctaa ggatgtaaaa acattttccc ttgtaagatg aggaaaaaat
     4141 ttgcaaaaat agaatgaaag cgttataata aaatcataaa aacaaaaatg tttagaaaga
     4201 aggtactcat atgattaaat atagtatccg tggtgaaaac ctagaagtaa cagaagcaat
     4261 tcgtgattat gtagtttcta aactcgaaaa gatcgaaaag tacttccaac cagaacaaga
     4321 gttggatgcc cgaattaact taaaagttta tcgtgaaaaa acggctaaag tggaagtaac
     4381 gattccgctt ggatctatta ctctccgcgc agaagatgta tctcaagata tgtatggttc
     4441 aattgacctt gtaactgata aaattgaacg tcagattcgt aaaaataaaa caaaaatcga
     4501 gcgtaaaaat aaaaataagg tagcaactgg tcaattattt acagatgctt tggtggaaga
     4561 ttcaaatgtt gtccagtcta aagttgttcg ttcaaaacaa attgatttaa aaccaatgga
     4621 tttggaagaa gcaattctac aaatggattt attggggcat gatttcttta tctatgtgga
     4681 tgttgaagat cagacaacca atgtgattta tcgtcgtgag gatggcgaaa ttggtttgtt
     4741 agaggttaaa gaatcttaat ttcaatatgt gtaaaggtag gtttactgaa ttgtaaacta
     4801 cctttttctt ataattgata gaattactga ttacactttt aaaaagtcgc tttttgaggg
     4861 gtattatggt ataatgggtg ccaagaggtt ttgaatgaaa aaattttatg taagtccaat
     4921 ttttcctatt ctagtaggat tgattgcgtt tggagtctta tccactttca ttatttttgt
     4981 taataataat ctgttgacgg ttttaatttt gtttcttttt gtaggaggct atgttttttt
     5041 atttaagaaa ctgagagtgc attatacaag gagtgatgta gaacagatac agtatgtaaa
     5101 ccaccaagcg gaagaaagtt tgacatctct attggaacaa atgcctgtag gtgttatgaa
     5161 attgaattta tcttctggag aggttgagtg gtttaatccc tatgctgaat tgattttgac
     5221 caaggaagat ggtgattttg atttagaagc tgttcaaacg attatcaagg cttcagtagg
     5281 aaatccgtct acttatgcca agcttggtga gaagcgttat gctgttcata tggatgcttc
     5341 ttccggtgtt ttgtattttg tagatgtatc cagggaacaa gccataacag atgaattggt
     5401 aacaagtcga ccagtgattg ggattgtctc tgtggataat tatgatgatt tggaggatgc
     5461 aacttctgag tcagatatta gtcaaatcaa tagttttgta gctaatttta tatcagagtt
     5521 ttcagaaaaa tacatgatgt tttctcgtcg ggtaagtatg gatcgatttt atctatttac
     5581 tgactacacg gtgcttgagg gcttgatgaa tgataaattt tctgttattg atgctttcag
     5641 agaagagtcg aaacagagac agttgccctt gaccttaagt atgggatttt cttatggcga
     5701 tggaaatcat gatgagatag ggaaagttgc tttgctcaat ttgaacttgg ctgaagtacg
     5761 tggtggcgac caggtggttg ttaaggaaaa cgacgaaacg aaaaatccag tttattttgg
     5821 tggtgggtct gctgcttcaa tcaagcgtac acggactcgt acgcgcgcta tgatgacagc
     5881 tatttcagat aagattcgga gtgtagatca tgtttttgta gtcggtcaca aaaatttaga
     5941 catggatgct ttgggctctg ctgtaggtat gcagttgttc gccagcaatg tgattgaaaa
     6001 tagctatgct ctttatgatg aagaacaaat gtctccagat attgaacgag ctgtttcatt
     6061 catagaaaaa gaaggagtta cgaagttgtt gtctgttaag gatgcaatgg ggatggtgac
     6121 caatcgttct ttgttgattc ttgtagacca ttcaaagaca gccttaacat tatcaaaaga
     6181 attttatgat ttatttaccc aaaccattgt tattgaccac catagaaggg atcaggattt
     6241 tccagataat gcggttatta cttatatcga aagtggtgca agtagtgcca gtgagttggt
     6301 aacggaattg attcagttcc agaattctaa gaaaaatcgt ttgagtcgta tgcaagcaag
     6361 tgtcttgatg gctggtatga tgttggatac taaaaatttc acctcgcgag taactagtcg
     6421 gacatttgat gttgctagct atctcagaac gcgcggaagt gatagtattg ctatccagga
     6481 aatcgctgcg acagattttg aagaatatcg tgaggtcaat gaacttattt tacaggggcg
     6541 taaattaggt tcagatgtac taatagcaga ggctaaggac acgaaatgct atgatacagt
     6601 tgttattagt aaggcagcag atgccatgtt agccatgtca ggtattgaag cgagttttgt
     6661 tcttgcgaag aatacacaag gatttatctc tatctcagct cgaagtcgta gtaaactgaa
     6721 tgtacaacgg attatggaag agttaggcgg tggaggccac tttaatttgg cagcagctca
     6781 aattaaagat gtaaccttgt cagaagcagg tgaaaaactg acagaaattg tattaaatga
     6841 aataaaggaa aaggagaaag aagaatgaaa gtaatctttt tagcagatgt taaaggaaaa
     6901 ggtaaaaaag gcgaaattaa ggaagtacca acagggtatg cgcaaaactt tcttatcaaa
     6961 aagaatctag ccaaagaagc gactgctcaa gctgtaggtg aacttcgtgg taaacaaaaa
     7021 tcggaagaaa aagctcacgc tgagatgatt gcagaaggga aagcaattaa agcacaactt
     7081 gaagcagaag aaactgttgt agaatttgtt gaaaaagttg gtccagatgg tcgtaccttt
     7141 ggttctatta ccaataagaa gattgcagaa gaattacaaa agcaatttgg aattaagatt
     7201 gataaacgtc atattcaagt acaagctccg attcgagcgg ttggtttgat tgatgtgcca
     7261 gtgaaaatct atcaagatat cacaagtgta atcaatcttc gtgtgaaaga aggataagtt
     7321 tacaccttct tgacaagatt gtaaaaggaa gggaagtctg atggcagaag tagaagagtt
     7381 acgagtacaa cctcaagata tcttagctga gcaatccgtt ttaggggcta tctttattga
     7441 tgagagtaaa cttgtttttg tgcgagaata cattgagtct cgggactttt ttaagtatgc
     7501 ccatcgtttg attttccaag ccatggtcga tttatccgat cgtggtgatg ccatagatgc
     7561 aacaacggtt cgtactatcc ttgataatca aggtgattta cagaatattg gtggcttgtc
     7621 ttacttggtt gagattgtca attctgtgcc aacttctgct aatgcggagt attatgctaa
     7681 gattgttgca gaaaaagcaa tgctacgtcg tttaattgcc aagttgacag agtctgtcaa
     7741 ccaagcttac gaagcgtcac aaccagctga tgaaattatt gctcaggcag aaaaagggct
     7801 gattgatgtc agtgaaaatg caaatcgaag cgggtttaag aacattcgag atgtgttgaa
     7861 tctcaacttt ggaaatctgg aagctcgctc gcaacaaacg accgatatta caggtattgc
     7921 gacaggttat cgtgatttgg atcatatgac aacaggactt catgaggagg agttgattat
     7981 cttagcagct cgtccagcag ttggtaagac agcatttgcc ttgaatatcg ctcagaatat
     8041 tgggactaag ttggacaaaa cggttgctat tttttcactc gaaatgggtg cggaaagctt
     8101 ggtagaccgt atgttagctg cagaaggctt ggtggagtca cattctatcc gtacagggca
     8161 attgacagat gaggagtggc aaaaatatac tattgctcag ggtaatctag ctaacgccag
     8221 tatctatatc gatgatacgc caggtattcg gattacagag attcgttctc gttctcgtaa
     8281 attggctcaa gaaactggaa atcttggttt gattttgata gactatttgc aacttatcac
     8341 gggaactggt cgagaaaatc gtcaacaaga agtttctgaa atttctcgtc agttgaaaat
     8401 actagccaag gaattgaagg ttccagtaat cgctctgagt cagctttctc gtggtgtaga
     8461 acaacgtcag gacaagagac cggtcttgtc tgatattcgt gaatctgggt ctattgagca
     8521 ggacgctgat atcgtagctt ttctctatcg cgatgactac tatgaacgtg gtggtgaaga
     8581 agaggagggt atcccaaata ataaggtgga agttattatc gagaaaaacc gtagtggagc
     8641 tcgtggaaca gtggaattga ttttccaaaa agaatacaat aaattttcaa gtatctcaaa
     8701 gagggaggca taagatgtca gatgcattta cagatgtagc caagatgaaa aaaatcaaag
     8761 aagaaatcaa ggcacatgag ggacaagtcg tagaaatgac tttggagaat ggtcgtaagc
     8821 gccaaaaaaa tagattaggt aagctaattg aagtttatcc atccctattt attgtggagt
     8881 ttggggatgt ggaaggagat aaacaagtta atgtttacgt tgaatccttt acttactcag
     8941 atattcttac agaaaagaat ttgattcatt atcttgacta aagtgagaaa ttttctcact
     9001 ttttcttttt tctccgaata atttaggtga aggcaatcat cgctttatat tatttttcaa
     9061 ggaggaagaa tgaaaatttt accgtttata gcaagaggaa caagttatta cttgaagatg
     9121 tcagttaaaa agcttgttcc ttttttagta gtaggattga tgctagcagc tggtgatagt
     9181 gtctatgcct attccagagg aaatggatcg attgcgcgtg gggatgatta tcctgcttat
     9241 tataaaaatg ggagccagga gattgatcag tggcgcatgt attctcgtca gtgtacttct
     9301 tttgtagcct ttcgtttgag taatgtcaat ggttttgaaa ttccggcagc ttatggaaat
     9361 gcgaatgaat ggggacatcg tgctcgtcgg gaaggttatc gtgtagataa tacaccgacg
     9421 attggttcca ttacttggtc tactgcagga acttatggtc atgttgcctg ggtgtcaaat
     9481 gtaatgggag atcagattga gattgaggaa tataactatg gttatacaga atcctataat
     9541 aaacgagtta taaaagcaaa cacgatgaca ggatttattc attttaaaga tttggatggt
     9601 ggcagtgttg ggaatagtca atcctcagct tcaacaggcg gaactcatta ttttaagacc
     9661 aagtctgcta ttaaaactga acccctagtt agtgcaactg tgattgatta ctattatcct
     9721 ggggagaagg ttcattatga tcagatactt gaaaaagacg gctataagtg gttgagttat
     9781 actgcctata atggaagtta tcgttatgtt caattggagg ctgtgaataa aaatcctcta
     9841 ggtaattctg ttctttcttc aacaggtgga actcattatt ttaagaccaa gtctgctatc
     9901 aaaactgaac ccctagttag cggaactgtg attgattact attatcctgg ggagaaggtt
     9961 cattatgatc agatacttga aaaagacggc tacaagtggt tgagttatac tgcctataat
    10021 ggaagtcgtc gctatataca gctagaggga gtgacttctt cacaaaatta tcagaatcaa
    10081 tcaggaaata tctctagcta tggatccaat aatagttcaa ctgtcggttg gaagaaaata
    10141 aatggtagtt ggtatcattt caaatcaaat ggttctaaat caacaggatg gctgaaagac
    10201 ggttctagct ggtattattt gaaattatct ggtgaaatgc agacaggatg gttaaaggag
    10261 aatggctcgt ggtattatct gggtagttca ggggcaatga aaacaggctg gtaccaggtc
    10321 tctggtaagt ggtattattc ttactcttca ggcgccttag ctgttaatac gacggtggat
    10381 ggctacagag taaacagtga tggagaacga gtatagaaaa ttggagtagg agaaatttcc
    10441 tgctcttttc tgtaagcagt ttccttgaca tttttcttat tttgcgctat catatataca
    10501 tataatatat gggagtctgt gtacagtctg agaggaagtg ttaaacttcg accgcacctg
    10561 atctgggtaa tgccagcgga gggaacgata cttagtctaa ttttgcacct tttccatgta
    10621 tggtaaaggt ttttcttttt tttaaaagga aaacgagaag aggaggttct tatgaaagca
    10681 agcattgcct tgcaagtttt acccctagca caggggattg atcggatagc tgttattgat
    10741 caggtcattg cttatctgca aactcaagaa gtgacgatgg tagtgacacc atttgaaacg
    10801 gtcttggaag gggagtttga tgagcttatg cgcattctaa aagaagcgct ggaagtggca
    10861 gggcaggagg cagacaatgt ctttgccaat gtcaaaataa atgtaggaga gattttaagt
    10921 attgatgaga aacttgagaa gtatactgag acgacacatt agtctattgg gctttctcgg
    10981 agtattgtca atctggcagt tagcaggttt tcttaaactt ctccccaagt ttatcctgcc
    11041 gacacctctt gaaattctcc agccctttgt tcgtgacaga gaatttctct ggcaccatag
    11101 ctgggcgacc ttgagagtgg ctttactggg gctgattttg ggagttttga ttgcctgtct
    11161 tatggctgtg ctcatggata gtttgacttg gctcaatgac ctgatttacc ctatgatggt
    11221 ggtcattcag accattccga ccattgccat agctcctatc ctggtcttgt ggctgggtta
    11281 tgggattttg cccaagattg tcttgattat cttaacaaca acctttccca tcatcgttag
    11341 tattttggac ggttttaggc attgtgacaa ggatatgctg actttgttta gtctgatgcg
    11401 agcaaaccct tggcaaatcc tgtggcattt taaaatcccg gttagcctgc cttactttta
    11461 tgcaggtctg agggtcagtg tctcctacgc ctttatcaca actgtggtat ctgagtggtt
    11521 gggaggtttt gaaggtcttg gtgtttatat gattcagtct aaaaaactgt ttcagtatga
    11581 taccatgttt gccattatta ttctggtgtc gattatcagt cttttgggta tgaagctggt
    11641 cgatatcagt gaaaaatatg tgattaaatg gaaacgtact taaaatagag cgtttttgaa
    11701 aaagaaaaga ggaaatcaaa atgaagaaaa catggaaagt gtttttaacg cttgtaacag
    11761 ctcttgtagc tgttgtgctt gtggcctgtg gtcaaggaac tgcttctaaa gacaacaaag
    11821 aggcagaact taagaaggtt gactttatcc tagactggac accaaatacc aaccacacag
    11881 ggctttatgt tgccaaggaa aaaggttatt tcaaagaagc tggagtggat gttgatttga
    11941 aattgccacc agaagaaagt tcttctgact tggttatcaa cggaaaggca ccatttgcag
    12001 tgtatttcca agactacatg gctaagaaat tggaaaaagg agcaggaatc actgccgttg
    12061 cagctattgt tgaacacaat acatcaggaa tcatctctcg taaatctgat aatgtaagca
    12121 gtccaaaaga cttggttggt aagaaatatg ggacatggaa tgacccaact gaacttgcta
    12181 tgttgaaaac cttggtagaa tctcaaggtg gagactttga gaaggttgaa aaagtaccaa
    12241 ataacgactc aaactcaatc acaccgattg ccaatggcgt ctttgatact gcttggattt
    12301 actacggttg ggatggtatc cttgctaaat ctcaaggtgt agatgctaac ttcatgtact
    12361 tgaaagacta tgtcaaggag tttgactact attcaccagt tatcatcgca aacaacgact
    12421 atctgaaaga taacaaagaa gaagctcgca aagtcatcca agccatcaaa aaaggctacc
    12481 aatatgccat ggaacatcca gaagaagctg cagatattct catcaagaat gcacctgaac
    12541 tcaaggaaaa acgtgacttt gtcatcgaat ctcaaaaata cttgtcaaaa gaatacgcaa
    12601 gcgacaagga aaaatggggt caatttgacg cagctcgctg gaatgctttc tacaaatggg
    12661 ataaagaaaa tggtatcctt aaagaagact tgacagacaa aggcttcacc aacgaatttg
    12721 tgaaataatg acagaaatta gactagagca cgtcagttat gcctatggtc aggagaggat
    12781 tttagaggat atcaacctac aggtgacttc aggcgaagtg gtttccatcc taggcccaag
    12841 tggtgttgga aagaccaccc tctttaatct aatcgctggg attttagaag ttcagtcagg
    12901 gagaattgtc cttgatggtg aagaaaatcc caaggggcgc gtgagttata tgttgcaaaa
    12961 ggatctgctc ttggagcaca agacggtgct tggaaatatc attctgcccc tcttgattca
    13021 aaaggtggat aaggcagaag ctatttcccg agcggataaa attcttgcga ccttccagct
    13081 gacagctgta agagacaagt atcctcatga acttagcggt gggatgcgcc agcgtgtagc
    13141 cttactccgg acctaccttt ttgggcacaa gctctttctc ttagatgagg cctttagcgc
    13201 cttggatgag atgacaaaga tggaactcca cgcttggtat cttgagattc acaagcagtt
    13261 gcagctaaca accctgatca tcacgcatag tattgaggag gccctcaatc tcagcgaccg
    13321 catctatatc ttgaaaaatc gccctgggca gattgttgca gaaattaaac tagattggtc
    13381 tgaagatgag gacaaggaag tccaaaagat tgcctacaaa cgtcaaatct tggcagaatt
    13441 aggcttagat aagtagaaaa atagggagtt ggtgaagatt atcctttacc agcgcccttt
    13501 ttcttttaaa aatgagaaaa tttcggtata atagtcaaac aaggtcaagg tttaaagaga
    13561 gaggtgggtt tgttatgaga tttaaaaata catcggatca tattgaggcc tacatcaagg
    13621 cgattttaga tcaatctggt atcgtggagt tgcaacggag tcagttggca gatacctttc
    13681 aggttgttcc tagtcagatt aactacgtga tcaagacacg ctttacggaa agtagaggct
    13741 acttggttga aagtaagcgt ggtggcggag gctacattcg tataggacgg attgagtttt
    13801 ctagtcatca tgaaatgctc cgggagctgc tttactcgat tggtgagcga gtcagtcaag
    13861 aaatttatga ggatattctc cagcttttgg ttgagcagga attgatgacc aagcaggaga
    13921 tgaatttgct agaatcagta gctttggatc gcgttttagg agaagaagct ccagttgttc
    13981 gagcaaacat gctacgtcag atcatacaag aggtagatag aaaagggaag taagatgaac
    14041 tattcaaaag cattgaatga atgtatcgaa agtgcctaca tggttgctgg acattttgga
    14101 gctcgttatc tagagtcttg gcacttgttg attgccatgt ctaatcacag ttatagtgta
    14161 gcaggggcaa ctttaaatga ttatccgtat gagatggacc gtttagaaga ggtggctttg
    14221 gaactgactg aaacggacta tagccaggat gaaaccttta cggaattgcc gttctcccgt
    14281 cgtttgcagg ttctttttga cgaagcagag tatgtagcgt cagtggttca tgctaaggtg
    14341 ctagggacag agcacgtctt ctatgcgatt ttgcatgatg gcaatgcctt ggcaactcgt
    14401 atcttggaga gggctggttt ttcttatgaa gacaagaaag atcaggtcaa gattgctgct
    14461 cttcgtcgaa atttagaaga acgggcaggc tggactcgtg aagacctcaa ggctttacgc
    14521 caacgccatc gtacagtagc tgacaagcaa aattctatgg ccaatatgat gggcatgccg
    14581 cagactccta gtggtggtct cgaggactat acgcatgatt tgacagagca agcgcgttct
    14641 ggcaagttag aaccagtcat cggtcgggac aaggaaatct cacgtatgat tcaaatcttg
    14701 agccggaaga ctaagaacaa ccctgtcttg gttggggatg ctggtgtcgg gaaaacagct
    14761 ctggcgcttg gtcttgccca gcgtattgct agtggtgacg tgcctgcgga aatgtctaag
    14821 atgcgcgtgt tagaacttga tttgatgaat gtcgttgcag ggacacgctt ccgtggtgac
    14881 tttgaagaac gcatgaataa tatcatcaag gatattgaag aagatggcca agtcatcctc
    14941 tttatcgatg aactccacac catcatgggt tctggtagcg ggattgattc gactctggat
    15001 gcggccaata tcttgaaacc agccttggcg cgtggaactt tgagaacggt tggtgccacc
    15061 actcaggaag aatatcaaaa acatatcgaa aaagatgcgg ctctttctcg tcgtttcgct
    15121 aaagtgacga ttgaagaacc aagtgtggca gatagtatga ctattttaca aggtttgaag
    15181 gcgacttatg agaaacatca ccgtgtacaa atcacagatg aagcggttga aacagcggtt
    15241 aagatggctc atcgttattt aaccagtcgt cacttgccag actctgctat cgacctcttg
    15301 gacgaagcag cagcaacagt gcaaaataag gcaaagcatg taaaagcaga cgattcagat
    15361 ttgagtccag ctgacaaggc cctgatggat ggcaagtgga aacaggcagc ccagctaatc
    15421 gcaaaagaag aggaagtgcc tgtctacaaa gacttggtga cagagtctga tattttgacc
    15481 accttgagtc gcttgtcagg aatcccagtt caaaaactga ctcaaacgga tgctaagaag
    15541 tatttaaatc ttgaagcaga actccataaa cgggttatcg gtcaagatca agctgtttca
    15601 agcattagtc gtgcgattcg ccgtaatcag tcaggaattc gcagtcacaa gcgtccgatc
    15661 ggttccttta tgttcctagg acccacaggt gtcggtaaga ccgagttggc caaggctttg
    15721 gcagaagttc tctttgatga cgaatcagcc cttatccgct ttgatatgag tgagtatatg
    15781 gagaaattcg cagccagccg tctaaatgga gctcctccag gctatgtggg ttacgaagaa
    15841 ggtggggagt taacagagaa ggttcgcaat aaaccctatt ccgttctcct ctttgatgag
    15901 gtagagaagg ctcacccaga tatctttaat gttctcttgc aggttctgga tgacggtgtc
    15961 ttgacagata gcaagggacg caaggtcgat ttttcaaata ccattatcat tatgacatcg
    16021 aatctaggtg cgactgccct tcgtgatgat aagactgttg gttttggggc taaggatatt
    16081 cgttttgacc aggaaaatat ggaaaaacgc atgtttgaag aactgaaaaa agcttataga
    16141 ccggaattca tcaaccgtat tgatgagaag gtggtcttcc atagcctatc tagtgaccat
    16201 atgcaggaag tggtgaagat tatggtcaag cctttagtgg caagtttggc tgaaaaaggc
    16261 attgacttga aattacaagc ttcagctctg aaattgttag caaatcaagg atatgaccca
    16321 gagatgggag ctcgcccact tcgcagaacc ctgcaaacag aagtggagga caagttggca
    16381 gaacttcttc ttaagggaga tttagtggca ggcagcacac ttaagattgg tgtcaaagca
    16441 ggccagttaa aatttgatat tgcataaaag aataaaagta tcagcatctg accataagtc
    16501 acagtggagt gaaactcaat gaaaatcaaa gagcaaacta ggcagctagc cgcaggttgc
    16561 ttaaaacact gttttgaggt tgcagataga gctgacgtgg tttgaagaga ttttcgaaga
    16621 gtatgaaact aaaacctata gcttctaaac gattcgtggt tttcaccatt caacacaaaa
    16681 ttcatatgtt tattaccctc cgtcgtattt gtcttagagc gtgtgtagta gaaaaagagc
    16741 agtcttatct gaaattttta ttctttcaaa agagacctgt ttcttttttg catgtcaaat
    16801 ccgttctaac tggtatttga aaaatcaaac taatactcaa tgaaaatcaa agaacaaact
    16861 aggcagctag ccgcaggttg ctcaaaacac tgttttgagg ttgtagatag agctgacgtg
    16921 gtttggagag attttcgaag agtataagct gcaagatgaa tgattttctt gtattgacgt
    16981 tgttgttgac aaaaagtagc ggataaatga aatccattct attatcatag atgataggct
    17041 ggtaggaaat tttcaaatag catacaggaa atagatgtat ggagttctgg tagtagaaag
    17101 ggagagagat gaacatttta gttgcagatg acgaggaaat gattagagaa ggaattgcag
    17161 catttctgac agaagagggt tatcatgtca ttatggctaa ggatggacaa gaggtcttgg
    17221 aaaaatttca agatctccct atccatctca tggtactgga tttaatgatg cctaggaaga
    17281 gtggttttga agtgttaaaa gaaatcaatc aaaagcacga tattcctgtc atcgtcttga
    17341 gtgctctggg agatgaaact actcagtcac aggtatttga tctctatgct gatgatcatg
    17401 tgacaaaacc tttttctttg gtactgcttg tcaagcgtat taaggcgctt atcagacgtt
    17461 actacgtcat agaggatatt tggcgatatc aggatgtaac agtggatttt acctcttaca
    17521 aagcacatta taaaaatgaa gaaattgatc tcaaaccaaa ggaattactg gtactaaagt
    17581 gtttgattca gcataaaaat caagttttaa gtagagagca gatattggaa gaaatttcaa
    17641 aagatgtagc tgatttacct tgtgataggg tcgttgatgt ctatattcgt actcttcgca
    17701 aaaaattagc tttagattgt atcgtgactg tgaaaaatgt tgggtataag attagcttat
    17761 gataaaaaat cctaaattat taaccaagtc ttttttaaga agttttgcaa ttctaggtgg
    17821 tgttggtcta gtcattcata tagctattta tttgaccttt cctttttatt atattcaact
    17881 ggagggggaa aagtttaatg agagcgcaag agtgtttacg gagtatttaa agactaagac
    17941 atctgatgaa attccaagct tactccagtc ttattcaaag tccttgacca tatctgctca
    18001 ccttaaaaga gatattgtag ataagcgcct ccctcttgtg catgacttgg atattaaaga
    18061 tggaaagcta tcaaattata tcgtgatgtt agatatgtct gttagtacag cagatggtaa
    18121 acaggtaacc gtgcaatttg ttcacggggt agatgtctac aaagaagcaa agaatatttt
    18181 gcttttgtat ctcccatata catttttggt tacaattgct ttttcctttg ttttttctta
    18241 tttttatact aaacgcttgc tcaatcctct tttttacatt tcagaagtga ctagtaaaat
    18301 gcaagatttg gatgacaata ttcgttttga tgaaagtagg aaagatgaag ttggtgaagt
    18361 tggaaaacag attaatggta tgtatgagca cttgttgaag gttattcatg agttggaaag
    18421 tcgtaatgag caaattgtaa aattgcaaaa tcaaaaggtt tcctttgtcc gcggagcatc
    18481 acatgagttg aaaacacctt tagccagtct tagaattatc ctagagaata tgcagcataa
    18541 tattggagat tacaaagatc atccaaaata tattgcaaag agtataaata agattgacca
    18601 gatgagccac ttattggaag aagtactgga gtcttctaaa ttccaagagt ggacagagtg
    18661 tcgtgagacc ttgactgtta agccagtttt agtagatatt ttatcacgtt atcaagaatt
    18721 agctcattca ataggtgtta caattgaaaa tcaattgaca gatgctacca gggtcgtcat
    18781 gagtcttagg gcattggata aggttttgac aaacctgatt agtaatgcaa ttaaatattc
    18841 agataaaaat gggcgcgtaa tcatatccga gcaagatggc tatctctcta tcaaaaatac
    18901 atgtgcgcct ctaagtgacc aagaactaga acatttattt gatatattct atcattctca
    18961 aatcgtgaca gataaggatg aaagttccgg tttgggtctt tacattgtga ataatatttt
    19021 agaaagctat caaatggatt atagttttct cccttatgaa cacggtatgg aatttaagat
    19081 tagcttgtag acagattagt tttttattaa agttcatata gggttaacat aagtgtgtta
    19141 ttctttgtgt agataaaaga aaggatacta atatggtatt agcgattatt ttagtaacat
    19201 tccttattcg attgattttt ttaaagcgtt cgatagagaa tgagaaacga atccttagca
    19261 atggcgggaa agaatttgga gttgagaata caaaacgatt aactatggct catattgttt
    19321 tttatctctc ttgcttggtt gaggcaatgg tgcacaagac aatttttgat ggcatgggca
    19381 tggttggttt agtcttgctt attttttcta tgctgatgtt gatgttggtg attcacttgt
    19441 tgggagatat ttggacagtg aagcttatgc ttgtcaataa tcacaaatat gtagatcata
    19501 ttttgtttag gacagtaaaa catcctaatt actttttaaa tattcttcct gagttgattg
    19561 gcttgacctt gttgagtcat gcttatgtga cttttgtttt agtttttcca gtttatgcag
    19621 ttattttgta tcgacgaata gctgaagagg aaaagctatt acatgaagtt ataatcccaa
    19681 atggaagcat aaagagataa atacaaaatt cgatttatat acagttcata ttgaagtgat
    19741 atagtaaggt taaagaaaaa atataggaga taattatgtt tgcatcaaaa aacgaaagaa
    19801 aagtacatta ttcaattcgt aaatttagta ttggagtagc tagtgtagct gttgctagct
    19861 tgttcttagg aggagtagcc catgcagaag gggttagaat tgggaataac tccacggtta
    19921 catctagtgg ggatgaagtc gagtcgcatc tacaaagtat attgaaggat gtcaataaaa
    19981 atttgaaaaa agttcaacat acccaaaatg tcggcttact cacaaagttg agcgaaatta
    20041 aacggaagta tttgtatgaa ttaaaagtta atggtttaga agagaagtcg aaagctgagt
    20101 tgacgtcaaa aacaaaaaaa gagttaaccg cagcttttga gcagtttaaa aaagatacat
    20161 tatcaacaga actagaaaaa aaggtagcag aagctcagaa gaaggttgca gaagctgaga
    20221 aaaaggccaa ggctcaaaaa gaagaagatc accgtaacta cccaaccaat acttacaaaa
    20281 cgcttgaact tgaaattgct gagtccgatg tggaagttaa aaaagcggag cttgaactat
    20341 tgaaagagga agctaaggaa tctcgagacg agggcacaat taagcaagca gaagcgaaag
    20401 ttgagagtaa aaaagctgag gctacaaggt tagaaaaaat caagacagat cgtaaaaaag
    20461 cagaagaagc taaacgaaaa gcagcagaag aagataaagt taaagaaaaa ccagctccag
    20521 caccacaacc agcgccggct cctcaaccag aaaaaccagc tgaagaaact ccagctccag
    20581 ctccaaaacc agagaagcca actgaacaac caaaagcaga aaaaccagat gatcaacaag
    20641 cagaagaaga ctatgctcgt agatcagaag aagaatataa tcgcttgact caacagcaac
    20701 cgccaaaacc agagcaacca gctcctgcac caaaaatagg ctggaaacaa gaaaacggta
    20761 tgtggtactt ctacaatact gatggttcaa tggcgacagg atggctccaa aacaacggtt
    20821 catggtacta tctaaacgct aatggtgcta tggcgacagg atggctccaa aacaatggtt
    20881 catggtatta cctcaacgct aatggttcaa tggcgacagg atggctccaa tacaatggtt
    20941 catggtacta cctaaacagc aatggcgcta tggcgacagg atggctccaa aacaatggtt
    21001 catggtacta cctcaacgct aatggttcaa tggcgacagg atggctccaa tacaatggtt
    21061 catggtacta cctcaacagc aatggtgcta tggtaacagg atggctccaa aacaatggct
    21121 catggtacta cctcaacgct aacggttcaa tggcaacaga ttgggtgaaa gatggagata
    21181 cctggtacta tcttgaagca tcaggggcta tgaaagcaag ccaatggttc aaagtatcag
    21241 ataaatggta ctatgtcaat ggctcaggtg cccttgcagt caacacaact gtagatagct
    21301 atagagtcaa tgccaatggt gaatgggtaa actaaaccta atataactag ttaatactga
    21361 cttcctgtaa gaactcttta aagtattccc tacaaatacc atatcctttc agtagataat
    21421 atacccttgt aggaagttta gattaaaaaa taactctgta atctctagcc ggatttatag
    21481 cgctagagac tacggagttt ttttggctct ttgtcaactg tagtgggttg aagaaaagtg
    21541 gctaagctcg agaaaggaca aatttcgtcc tttctttttt gatgttcaaa gcgataaaaa
    21601 tccgtttttt gaagttttca aagttccgaa aaccaaaggt attgcgtttg atgagtttaa
    21661 tgagattatt ggtcgcttcc agtttggcgt tagaatagtg tagttgaagg gcgttgataa
    21721 tcttttcttt atctttgagg aaggttttaa agacagtctg aaaaatagga tgaacctgct
    21781 taagattgtc ctcaatgagt ccgaaaaatt tctctggttc cttattctgg aagtgaaaaa
    21841 gcaagagttg atagagatca tagtggtgtt tcaagtcttc cgaatagctc aaaagcttgt
    21901 ttaaaatctc tttattggtt aagtgcatac gaaaagtagg acgataaaat cgcttatcac
    21961 tcagtttacg gctatcctgt tgaatgagtt tccagtagcg cttgatagcc ttgtattcat
    22021 gggattttcg ctcaaactga ttcatgattt ggacacgcac acgactcata gcacggctta
    22081 gatgttgtac aatgtgaaag cgttcaaaaa cgatttcgct atattcgaga ggcttttcgt
    22141 gatgacgaat tgacctctgt ctgcaagtta gatgaaatca atcttccccg ttaccctatg
    22201 ggctggcagg agtggttaga ccagatgtta gcggaaattt cctatgatga aaaccagcaa
    22261 gagctgatct tctttgtagg agaagcagac tatcagcaag aactatctaa acgtggattt
    22321 ggcactgtct tgccagaaag aaagtttggt atctcggtga ctatgattcg agaaaatcca
    22381 agcaaatatt ggaaatacat cgctcaaccc ttccgtcgtc agtttaccaa gaaagtattg
    22441 atcatgggaa gtgccagcaa tgggaagacc actctagcca aggatttggc taggtattac
    22501 gatgcgcctg tcagtctgga atacgcacgt gagtatcaga ttaaaaataa cgtccgcgac
    22561 gatgaattga ctccaaagga ttactactat ctcctgttgg gacagtatga ccagacctca
    22621 aagttaatcg atagcaatgc caatcgaggt ttggtaatag ccgataccaa ctccttggta
    22681 accaagggct actatgatta ttacatggag acagagaacc aagtggactt atcaggagaa
    22741 acctttgaca atctctttgt ttcaatcttg gccaaggaaa aatgggactt gattctcttt
    22801 gtgcaacctg ttggctccta tgtcaatgac ggatttagag atatgaccat ggcagaagag
    22861 cacattcgct acagtttttc tcagcatttg gaccagatga gggagagata cttaacgact
    22921 attcctttag tttatctagc ggaggattat ctaggtaatt atgaagcagc aaaagtgact
    22981 attgatgcca tttaccaagc agattaggag aaaattatga aaaagctaac tgaaaaaatc
    23041 acacaattta tcgaaaactt taaaaatgtc catgccaaag cccgaaagat tggttttgca
    23101 gggttcatgc gcctgctctg gaaagatctc tttgttggtc gtagcctttt ccagtggttg
    23161 tatctcatcg ccttgtcaag tgttcccttg atcttaaagt tcacgcaaaa tacaaaaagt
    23221 catgactggc tgagcttgtt tgcatcctgg actgggattg tctgtgtcat cttggtagca
    23281 gaaggacgtg caagcaatta tctctttggg gctattaatt cggctatcta tttgattttg
    23341 gccatgaatg cgacttttta tggtgaagtt ttgacgacag tttacttctt tgtcatgcag
    23401 ccgattggtc tctgtgcttg gttgtccaat cgtatcaatg accaaggtaa accagaagaa
    23461 tcccactttg aagctaagaa attatctgtt cttgactggc tcaagtactt aaccttgact
    23521 gccatcatct ggattggtat gggcttggct taccaaagta tcaatagcgc tcgtcctttc
    23581 cgtgatagtg ttacctgttg gtcagctctt gatgacacgt ctcaaccgtg agcaatggat
    23641 tttctggatt gcaaccaatc tctttagtat ctatctctgg tggggcgaaa atattcacat
    23701 ccaagggatg tactgggttt acacattcaa tagtctagtg tgacggtgga tatggtggcc
    23761 tactgctttg tcgaaggaaa aatcaaactc ttgctgattc gtcgcaaggc tcacccttat
    23821 caaaactatt tagccttagt tggaggcttt atggacaagg gagaagatgc tgcacatgcc
    23881 tatcagcgcg aagtgaaaga agaagtcaat ctcgatcttc ctttggaaaa aatcgagcag
    23941 ttaatgaccg tatcgacccc tggtcgtgat ccacgggggt ggacagtgac cattgcctat
    24001 ttagtgtacc tacctagtcg tgcattagaa ctggttcagg caagtgacga tgctaaggat
    24061 gttgtttttg tagatgttga ctttcagacg gggaaatgct accttgaggg agtggagctg
    24121 gacgagcaag ccttagcctt tgaccattat gccattatcc aaaaatccat caaacgaatc
    24181 caaggccgta tcgactggaa cccgaccttc ctttatctgt tggaagaaga gtttactgtc
    24241 tatgaaggaa ccgaactggt caatttcatc aaccccggtc gcccaatcgt cagcaataac
    24301 tttctcgtaa aatacggcga atatgtagaa gaagtcggac tcaaacgagt acctaaaaag
    24361 aaaccaagaa aaacctatcg attgaaataa agaggtcgag acaaagttcc cgacttcttt
    24421 atttatcatc attttttatg aatggctatc tctcttagaa ttacacaaga gcaatgcaaa
    24481 aaataggact ctaatctaag aaattttcta ttttttcgtt tcgattacga attgaaatag
    24541 tggaggcaac aatttttaaa ataggttctc taaatagttg acagattcaa agtataatgt
    24601 tacaattatg ttacaaaaag atttattaac atttcaaaaa attacaaaag agtagaaaaa
    24661 tacaatagaa aacctctcta aaattgatgg ctgttactgt attggcttct agtttgctgt
    24721 taggagcttg tggaaataaa aatgaaacca ctcaaactag cagctctgca aagaccagcc
    24781 aatcatcaag ttctaaagca acttcttcta acaaagaaag caagactcaa aaatcttctg
    24841 atcagtcttc tacaacggca gatcagtcgc aagcggcacc agcacctgaa tcaagacaag
    24901 aacaaacagc tcctagtcaa caagctccat ctcaggtgcc ttcaactcat caaggttctc
    24961 aagcccagtc taaccagtca acttacgacc caacaactga ccacaaactt caagacaagc
    25021 aagctgaaca caacaaacgc taaaagggag ttttaaccat ggtcgatggt gatttctcag
    25081 ctgcagcagg aaactggaag ggagcgagtg gcgaaatcat tacggtttca tcagggggta
    25141 attcacagta gaaactgcag atggaaagaa agaaaacgat tctatcagag gctactctta
    25201 cactcttgat gatggcaagt ataatgctaa aatcggagga ggaaaaatca tccaaattac
    25261 aacaggtgcg gatggtaaag tttcgagtgt tgccttgatt caataatagt atctaggatt
    25321 tcctaggata aaaaaaccgt aaacttcaag ggcactgcac cccaataatg ggacaagaga
    25381 aaaacctctt atgcgaccag ttttctgtac tctacaggag actggtcatt taatttctgt
    25441 tgaattctag tttcattaga atagcagagg aagaaaaatt attgcaggaa gttattattc
    25501 caaatggaag aataaaagga tagcgaaaat aatgtagcaa atttcatata caattcatat
    25561 tagcaggata tactgtagtt aattaaaatt atggagaata tagacatgtt taaatcaaat
    25621 catgagcgaa gaatgcgtta ttccattcgt aaatttagtg taggagtagc tagcgtagct
    25681 gttgccagtc tttttatggg aagtgttgta catgcgacag agaaagaggg aagtacccaa
    25741 gcagccactt cttttaatag gggaaatgga agtcaggcag aacaacgtgg agaactcgat
    25801 tcagaacgag ataaggcaag gaaagaggtc gaggaatatg taaaaaaaat agtgggtgag
    25861 agctatgcaa aatcaactaa aaagcgacat acaaatactg tagctctagt taacgagttg
    25921 aacaacatta agaacgagta tttgaataaa atagttgaat caacctcaga aagccaacta
    25981 cagatactga tgatggagag tcgatcaaaa gtagatgaag ctgtgtctaa gtttgagtct
    26041 aagtttgaaa aggactcatc ttcttcgtca agttcagact cttccactaa accagaaact
    26101 ccgcagccgg aaaatccaga gcatcaaaaa ccaacaactc cagctccgga taccaaacca
    26161 agccctcaac cagaaggcaa gaaaccaagc gtaccagaca ttaatcagga aaaagaaaaa
    26221 gctaagcttg ctgtagcaac ctacatgagc aagattttag atgatataca aaaacatcgt
    26281 cagattgttg ctcttattaa ggagcttgat gagcttaaaa agcaagctct ttctgaaatt
    26341 gataatgtaa ataccaaagt agaaattgaa aatacagtcc acaagatatt tgcagacatg
    26401 gatgcagttg tgactaaatt caaaaaaggc ttaactcagg acacaccaaa agaaccaggt
    26461 aacaaaaaac catctgctcc aaaaccaggt atgcaaccaa gtcctcaacc agaggttaaa
    26521 ccgcagctgg aaaaaccaaa accagaggtt aaaccgcagc cggaaaaacc aaaaccagag
    26581 gttaaaccgc aaccggaaaa accaaaacca gaggttaaac cgcagccgga aaaaccaaaa
    26641 ccagaggtta aaccgcagcc ggaaaaacca aaaccagagg ttaaacc
//
LOCUS       DAVMGZ010000014        24424 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000014 DAVMGZ010000000
VERSION     DAVMGZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.015"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(189..647)
                     /locus_tag="VYQ93_000492"
     CDS             complement(189..647)
                     /locus_tag="VYQ93_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV3335233.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(644..1084)
                     /locus_tag="VYQ93_000493"
     CDS             complement(644..1084)
                     /locus_tag="VYQ93_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3335234.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1290..3239
                     /gene="mutL"
                     /locus_tag="VYQ93_000494"
     CDS             1290..3239
                     /gene="mutL"
                     /locus_tag="VYQ93_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV3335235.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3564..4031)
                     /locus_tag="VYQ93_000495"
     CDS             complement(3564..4031)
                     /locus_tag="VYQ93_000495"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV3335236.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEISLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4032..5237)
                     /locus_tag="VYQ93_000496"
     CDS             complement(4032..5237)
                     /locus_tag="VYQ93_000496"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV3335237.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5257..5892)
                     /gene="ribE"
                     /locus_tag="VYQ93_000497"
     CDS             complement(5257..5892)
                     /gene="ribE"
                     /locus_tag="VYQ93_000497"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV3335238.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5877..6977)
                     /gene="ribD"
                     /locus_tag="VYQ93_000498"
     CDS             complement(5877..6977)
                     /gene="ribD"
                     /locus_tag="VYQ93_000498"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV3335239.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRKSPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNGAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7112..7226)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7383..7976
                     /gene="ruvA"
                     /locus_tag="VYQ93_000499"
     CDS             7383..7976
                     /gene="ruvA"
                     /locus_tag="VYQ93_000499"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV3335240.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASVENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            7986..8549
                     /locus_tag="VYQ93_000500"
     CDS             7986..8549
                     /locus_tag="VYQ93_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV3335241.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPVKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8546..9223
                     /locus_tag="VYQ93_000501"
                     /pseudo
     CDS             8546..9223
                     /locus_tag="VYQ93_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(9364..10395)
                     /locus_tag="VYQ93_000502"
     CDS             complement(9364..10395)
                     /locus_tag="VYQ93_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV3335242.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEEPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDEIFHDDYKEALLDIIDNNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(10650..11333)
                     /locus_tag="VYQ93_000503"
     CDS             complement(10650..11333)
                     /locus_tag="VYQ93_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV3335243.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPH
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11345..12289)
                     /locus_tag="VYQ93_000504"
     CDS             complement(11345..12289)
                     /locus_tag="VYQ93_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV3335244.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12421..15252
                     /gene="uvrA"
                     /locus_tag="VYQ93_000505"
     CDS             12421..15252
                     /gene="uvrA"
                     /locus_tag="VYQ93_000505"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV3335245.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKNVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMTAFGVDMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVVTNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15245..16306
                     /locus_tag="VYQ93_000506"
     CDS             15245..16306
                     /locus_tag="VYQ93_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV3335246.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16438..16836
                     /gene="spx"
                     /locus_tag="VYQ93_000507"
     CDS             16438..16836
                     /gene="spx"
                     /locus_tag="VYQ93_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV3335247.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            16902..17471
                     /locus_tag="VYQ93_000508"
     CDS             16902..17471
                     /locus_tag="VYQ93_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV3335248.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            17558..17824
                     /locus_tag="VYQ93_000509"
     CDS             17558..17824
                     /locus_tag="VYQ93_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV3335249.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            17828..18247
                     /gene="ruvX"
                     /locus_tag="VYQ93_000510"
     CDS             17828..18247
                     /gene="ruvX"
                     /locus_tag="VYQ93_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV3335250.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18263..18568
                     /locus_tag="VYQ93_000511"
     CDS             18263..18568
                     /locus_tag="VYQ93_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV3335251.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            19179..20429
                     /locus_tag="VYQ93_000512"
     CDS             19179..20429
                     /locus_tag="VYQ93_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV3335252.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISIFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            20426..20956
                     /locus_tag="VYQ93_000513"
     CDS             20426..20956
                     /locus_tag="VYQ93_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV3335253.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDGTYTG
                     QDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNGNQIV
                     VDDMDRDPSDQIVLTK"
     gene            21102..22634
                     /gene="cls"
                     /locus_tag="VYQ93_000514"
     CDS             21102..22634
                     /gene="cls"
                     /locus_tag="VYQ93_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV3335254.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22892..>24424
                     /locus_tag="VYQ93_000515"
     CDS             22892..>24424
                     /locus_tag="VYQ93_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV3335255.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSLRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKI"
BASE COUNT         7105 a         4679 c         5311 g         7329 t
ORIGIN      
        1 aaataactgt tatgatatag cagattgaaa ctagaatagt acacttttgc ttctaaaaca
       61 ttgttagaaa tcgatttgac tgtcctgatt gatttatcct gttcttattt catttcacta
      121 tagtaaggtg cagcaggata aagcaaaacc gagaagtttc tttctcggtt ttgcttatta
      181 tgaggttcct attttctatg ctccaagagt tgattgatgt gatctacttt tttagcctct
      241 cttttataga gaatagccca gatgatgaga tagacaatcg caaactcgat aatgagctgg
      301 aggtagaaaa tccagtggaa ggggaaccaa ccagcaagag ttgctagtgg gacaaagcca
      361 gccagcataa ggaagaaatg agtcagagtg gcacgaagca tgctccagtc acggctgaat
      421 aagcggttgc caaagttgaa gagcataccg atggctgccc agataagtgt gcagtagagc
      481 aagaccaggg caccgtgaac ctgatgctga tccatcactt ggccgatgag agagtaggga
      541 tttagtggtg cgtaggtatt tggtgcataa atgagtgaaa agaggatgga gaggatgagg
      601 ccgataagaa caccggcagc tgcatcgtga aatacttgtt ttttcatagt tctaatttct
      661 ccttgatggt ttttagataa cggcgtgaag agtaggtgaa gctttcgttt ttcaagaaaa
      721 tttctaccag accgtttggc gtgagcttga ggtgagagat ggaatcgata ttgatgattt
      781 ctgattggga aatttggata aaattgcttg gcaagagttt aagaacctga tagagtcgca
      841 aatcaatgct gtaggtctga ctcgcggttt ctgctagaac cttccgattc tcgatataga
      901 agcgctgaat cttgccaatc tcaactagat agacctgatc atcgattttt cctttgattt
      961 tttctctttg gtccagattt tctgcgaact cgatgacttt ctggactttt tcggtttctt
     1021 gaggtgcttg gacaatcagc ttttcctcct cgtaagtctc actaatctgt agttctactt
     1081 tcatagtttt ctctcctttt cagttataca aggttgtgat cacttcctgt atatccttat
     1141 taaacactat tttatagccc atggcaagga accttgtcta tgtggaggga ttgggctcct
     1201 atgtggtgga gcttttctgt tctttctgaa atatggtata atagcactaa ttaatttcta
     1261 ggaaaataga tacagaaagg ggctgaaaga tgtctcatat tattgaattg ccagagatgc
     1321 tggcaaacca aatcgcagct ggagaggtca ttgaacgtcc tgccagtgtg gtcaaagagt
     1381 tggtagaaaa tgccattgac gcgggctcta gtcagattat cattgagatt gaggaagctg
     1441 gtctcaagaa ggttcaaatc acggataacg gtcatggaat tgcccacgat gaggtggagt
     1501 tggccctgcg tcgccatgcg accagtaaga taaaaaatca agcagatctc tttcggattc
     1561 ggacgcttgg ttttcgtggt gaagccttgc cttctattgc gtctgttagt gtcttgactc
     1621 tgttaacggc ggtggatggt gctagtcatg gaaccaagtt agtcgcgcgt gggggtgaag
     1681 ttgaggaagt catcccagcg actagtcctg tgggaaccaa ggtttgtgtg gaggatctct
     1741 ttttcaacac gcctgcccgt ctcaagtata tgaagagcca gcaagcggag ttgtctcata
     1801 tcattgatat tgtcaaccgt ctgggcttgg cccatcctga gatttctttt agcttgatta
     1861 gtgatggcaa ggaaatgacg cggacagcag ggactggtca actgcgccaa gcaatcgcag
     1921 ggatttacgg tttggtcagt gccaagaaga tgattgaaat tgagaactct gacctagatt
     1981 tcgaaatttc aggttttgtg tctttacctg agttgactcg ggctaaccgc aattatatca
     2041 gcctcttcat caatggccgt tatatcaaga acttcctgct aaatcgtgct attttggatg
     2101 gtttcggaag caagcttatg gttggacgtt ttccactggc tgtcattcac atccatatcg
     2161 acccttatct agcggatgtc aatgtgcatc caactaagca agaggtgcgg atttccaagg
     2221 aaaaagaact gatgaccctg gtttcagaag ctattgcaaa tagtctcaag gaacaaacct
     2281 tgattccaga tgccttggaa aatcttgcca aatcgaccgt gcgcaatcgt gagaaggtgg
     2341 agcaaactat tctcccactc aaagaaaata cgctctacta tgagaaaact gagccgtcaa
     2401 gacctagtca aactgaagta gctgattatc aggtagaatt gactgatgaa gggcaggatt
     2461 tgaccctgtt tgccaaggaa accttggacc gattgaccaa gccagcaaaa ctgcattttg
     2521 cagagagaaa gcctgctaac tacgaccagc tagaccatcc agagttagat cttgctagca
     2581 tcgataaggc ttatgacaaa ctggagcgag aagaagcatc cagcttccca gagttggagt
     2641 ttttcggaca aatgcacggg acttatctct ttgcccaagg gcgagatgga ctttacatca
     2701 tagatcagca cgctgctcag gaacgggtca agtacgagga gtaccgtgaa agcattggca
     2761 atgttgacca gagccagcag caactcctag tgccctatat ctttgaattt cctgcggatg
     2821 atgccctgcg tctcaaggaa agaatgcctc tcttagagga agtgggcgtc tttctagcag
     2881 agtacggaga aaatcaattt attctacgtg aacatcctat ttggatggca gaagaagaga
     2941 ttgaatcagg catctatgag atgtgcgaca tgctcctttt gaccaaggaa gtttctatca
     3001 agaaataccg agcagagctg gctatcatga tgtcttgcaa gcgatctatc aaggccaatc
     3061 atcgtattga tgatcattca gctagacaac tcctctatca gctttctcaa tgtgacaatc
     3121 cctataactg tcctcacgga cgtcctgttt tggtgcattt taccaagtcg gatatggaaa
     3181 agatgttccg acgtattcag gaaaatcaca ccagtctccg tgagttgggg aaatattaaa
     3241 agtataaaaa agtctgggaa aaattttcaa aatcaaaaaa acgcataaaa tcaggtgttc
     3301 aaaaaccttg attttatgcg ttttatcatg gaaatagtta cttcattttt tcctaattct
     3361 tttcgaaact ctttttaaac gacgtcagtt ttatcagtaa tctcaaaaca gtgttttgag
     3421 ctaattttgc cagttttgtc tgtaacatcg aagttgtgtt ttaccactct gcgactggtt
     3481 tcctagtttg ctctatgatt ttcacagagt attaaattgc gattttgcca agtttcttta
     3541 ttcgtctaaa agtagagtct gttctatgcg tctaatgtac gaattaggtt gaccatttca
     3601 atagctcctt gtgcacactc agaaccctta tttcctgctt tagtaccagc tcgttctatg
     3661 gcttgttcaa ttgtatctgt cgttagcaca ccaaacataa caggaatttc gctatttaaa
     3721 ctgatttggg cgattccctt agatacctcg ctacatacat aatcataatg acttgtattt
     3781 cctctaatga cagctcccaa gcagataatt gcatcatatt ttttactttt tgccattttt
     3841 gatgcaatca gtgatatttc aaaagctcct ggaacccagg ctacctcgat atctttctcg
     3901 tttacattct ctcttttgag attatctagt gctccagata ataattttga agttataaat
     3961 tcattaaatc tcgctacaac aatacctatt ttaatattgt ttgctactaa attaccttca
     4021 taagtgttca tttatttttc ctccatattt aaaatgtgac ccattcgatt tttctttgtt
     4081 tctaaataaa aactatcgta aggattggct tctatttcga ttgatattct actggaaatg
     4141 gtaattccat atttttctaa ctgttcaacc ttgtcaggat tatttgtcag taaatgaagt
     4201 gactgaagtc ccagatcttt aagcatttgt gctccaatat gatattctct taaatcacct
     4261 tcaaagccta atgcaagatt ggcatcaagc gtatccatgc cttgatcttg taaatgatag
     4321 gcttttaatt tattgataag tccaattcct cgtccctcct gtcgcaagta aagtaagaca
     4381 cccgaaccat tctcaacaat cattttcata gctttatcga attgctgtcc acaatcgcaa
     4441 cgtaaggagc ctaaaacatc ccctgttaaa cattcggagt ggacccgaca taatacattg
     4501 gcttcatcct ctatgtttcc cataataaga gcaagatgat gttccccatt tagtttatct
     4561 atatagctaa ttgctttgaa attaccgtat ctagtaggca tattgacagt tgaaactcgt
     4621 tctaccagct gatcatatac ttttctatat tcttgtaatt ctttgatggt aattagtgga
     4681 atgttgtgtt tcttcgagaa ctgaattaaa tcatctgttc tcatcatttt gccatcatga
     4741 ttcattattt cacaacatag gccacactct tttagtccag ctaattttaa taaatcaaca
     4801 gttgcttctg tgtgtccatt tctttctaga acaccacctt tttttgcaat taaaggaaac
     4861 atgtgtcctg gcctgcgaaa atcagagggt gttatatctt cagctacaca catacgtgcg
     4921 gtcagtcctc tttcctcggc agaaatacct gtggtcgttt ctttataatc aattgaaact
     4981 gtaaaagcag tcttatgatt atctgtattg ttttcaacca taggtgaaag cattaattga
     5041 ttagctaaac tttcgctcat aggcatacaa attaatcctt tggcataagt agccataaaa
     5101 ttaacatttt ctgttgtagc tgcttgtgca gaacaaatta agtctccttc attttctcta
     5161 tccttgtcgt ctataacaag aacaagtcgt cccttctgca atgcttctaa tgcttcttgt
     5221 atttttcgat attccattga ctgattatcc tttctgctaa aatccatttt gatataatag
     5281 ttccttagat atttctgatt ttggagagtt atccatcagt ttttgcacat atttacctaa
     5341 gatatcattt tcaagattta ctgtactccc gacttgttta ctcttaagaa tggtttgttc
     5401 caaggtatga gggataacag atactgaaaa gtttactttg gagactttag cgacagtcag
     5461 actaatgccg tcaattgtaa tagatccttt ttcaactatt aaatctaaaa tttctttttg
     5521 tgtgttgatt tgataccata cagcattatc atcttttttt attgacgaga tttttcctgt
     5581 accatcaatg tgtcctgtaa cgacgtgacc cccaagtcga ccgttgacag ataaggctct
     5641 ttctagattc acctcacttc catgttttaa tagagtaaga gctgttcgac tccatgtttc
     5701 attcattaca tcaactgtaa aggattgatg attgaaatga gtaactgtaa gacagatacc
     5761 atttactgct atactatcgc ctaaatggat atccgttaat atttttgagg ctttaattga
     5821 tagtttacaa ttacgagagt ctttctgtat tctttcaact tttccgattt cttcaattat
     5881 tcctgtgaac atggataaat cacttcactt tctatgagat agtcatttcc tatttgagaa
     5941 aatgcataag gtttcaatct aatagcgcca tttggcaaag aaataccttc acctccgaca
     6001 ggaaacttgg cactacctcc aaaaattttt ggtgcaatat atattttcag ctcatcaaca
     6061 atttgttgtt ccaaagcact ccaattcatt agactgcccc cttctagaac taggctatca
     6121 atctgcatgt ttcctagatg ttgcattaaa ctcgataagt ctatatgatt gccttttttc
     6181 tttatggaaa gtatttcaca gccatgattt tgatatagct tcattttatt tttgtcttca
     6241 gaggaagtgg caatgtaagt tttaatatca tttgctgttt ttacgatttt agaggtaaga
     6301 ggagttcgta aatgtgtatc gcatatgata cggataggat ttttcccttc ctccaatcta
     6361 catgtcagca aaggatcgtc ttgaataaca gtattgactc ccaccataat tgcactaaca
     6421 tagtgtcgta actgatgcac atgctttctt gcttcttctt cagtaatcca tttggattga
     6481 tttgttttag tggctatttt tccatccatt gacattgcat atttcataaa aacatagggt
     6541 acatgctggg taatatactt tctaaaactt tttattaagt taagacactc attttctaaa
     6601 attccaacag taacttgaag attattttcc tcaagtatct ttactccttt tccagataca
     6661 ataggattac agtctaggct tccaatgact actcttgtaa taccactatc gattatagca
     6721 tctatacagg gaggtgtttt cccgaagtga caacagggtt caagtgttac ataaagcgtc
     6781 gctccgacag gggattttct acagttttta agagcatttc tctcagcatg tgggccacca
     6841 aaaaactcat gataaccttg tccgatgatg tgattatctt ttacaataac tgcgccgacc
     6901 ataggattgg gattgacgta accagcccct ttttgtgcca gttttattgc taatttcata
     6961 tattttgaat cgctcatctc actacctcca aaaaaaatat accttgaata ggggactact
     7021 caaggcatac aaaagaaaac ttatacgatt aacaaaaata ctctgaaatg acaagtaatc
     7081 atttcagagc acgcaaaaag cacaaatata cttttatctt ctttcatcca gactatactg
     7141 tcggctttgg aatttcacca aatcatgcct ttcggctcgt gggctatacc accggtaggg
     7201 aattgcaccc tgccctgaag atagttattc aattacagat gattatagta cttaattttg
     7261 aatatgtcaa cagataaata ccgattgttt ttgatatact gtatttgtga taatcgattc
     7321 tcgctcctcg gataaagaaa atatgatata ctagataaac gaaataagag agaaggaata
     7381 ctatgtacgc atatttaaaa ggaatcatta ccaaaattac tgccaaatac attgttcttg
     7441 aaaccaatgg tattggttat atcctgcatg tggccaatcc ttatgcctat tcaggtcagg
     7501 ttaatcagga ggctcagatt tatgtgcatc aggttgtgcg tgaggacgcc catttgcttt
     7561 atggatttcg ctcagaggat gagaaaaagc tctttcttag tctgatttcg gtctctggga
     7621 ttggtcctgt atcagctctt gctattattg ctgctgatga caatgctggc ttggttcaag
     7681 ccattgaaac caagaacatc acctacttga ccaagttccc taaaattggc aagaaaacag
     7741 cccagcagat ggtgctggac ttggaaggca aggtagtagt tgcaggagat ggccttcctg
     7801 ccaaggtcgc agtgcaagca agtgttgaaa accaagaatt ggaagaagct atggaagcca
     7861 tgttggctct gggctacaag gcaacagagc tcaagaaaat caagaaattc tttgaaggaa
     7921 cgacagatac agctgagaac tatatcaagt cggcccttaa aatgttggtc aaataggagc
     7981 agagaatgac aaaacgttgt tcgtgggtca agatgaccaa cccactctac atcgcctatc
     8041 atgatgagga gtggggccag cccctccatg atgaccaagc gttgtttgag ttgttgtgta
     8101 tggaaaccta tcaggcaggc ctgtcttggg aaacggtact caacaaacgc caagctttcc
     8161 gagaagcctt tcatagctat caaattcact cagtcgcaga gatgactgac actgaattgg
     8221 aagccatgct ggagaatcca gctatcattc gaaatagagc caagattttt gctacacgcg
     8281 ctaacgccca agcttttcta cagttacagg cagagtacgg ctcttttgat gcctatcttt
     8341 ggtcttttgt tgaggggaaa actgtcgtta acgatgttcc tgattatcgc caagccccag
     8401 ttaaaacacc cttatctgag aaattagcca aagatctcaa aaaacgaggc ttcaagttca
     8461 caggcccagt cgccgtattg tcttttctac aggctgcagg gctagttgat gaccacgaga
     8521 atgattgtga gtggaaaggt cttaaatgat gtctaacaaa aataaggaaa ttctgatttt
     8581 tgcgattctc tatacagtcc tctttgtgtt tgatggcgtt aaattgctgg cttctttaat
     8641 gccatctacc attgcaaatt atcttgttta tgtagtttta gctttatatg gctccttctt
     8701 gttcaaggat agattgatcc aataatggaa ggagattaga aagactaaaa gaaaattctt
     8761 ctttggagtc ttaacaggat ggctctttct cattctgatg actgttgtct ttgaatttgt
     8821 atcagagatg ttgaagcagt ttgtgggact agatggacaa ggtctaaatc agtctaatat
     8881 tcaaagtacc tttcaagaac aaccactact gatagctgtt tttgcttgtg tcattggacc
     8941 tctgatagaa gaattatttt tccgtcaggt cttattgcat tacttgcagg aacggttgcc
     9001 aggtttacta agcattattc tggtaggact tgtttttgct ctgactcata tgcacagttt
     9061 ggctctatca gagtggattg gtgcagttgg ttacttaggt ggaggccttg ccttttctat
     9121 tatttatgtg aaagaaaaag aaaatatcta ctatccccta cttgttcaca tgttaagcaa
     9181 cagcctctcc ttaatcattt tagttatcag tatagtaaaa tgaaataaga acaggacagt
     9241 caaatcgatt tctaacaatg ctttagaagt agaggtgtac tattctagtt tcaatacact
     9301 gtaacatgtg atgaaaatgc cagtaatgat accgagaaaa aagctgagaa acttttccca
     9361 gctttatttg ttatagtcaa agcgaatgac ttgttcctgt gcatctacat gagcatggac
     9421 cccaaagggt acaattgctc ttggagttgc gtggccgaca ttcagattat agacaatcgg
     9481 gatattgttg tcaatgatat ccaatagtgc ctctttatag tcgtcatgga aaatttcatc
     9541 cataggtttt ccgaccaaga gtccattgat gaccgcgaat atgccagtgt cctttaaagt
     9601 tagcaacatc tttttgaagt cttctggctt aggctcttct tcgcttgttt cgagcaagag
     9661 gattttccct tcccagtctg acaagtcagg gaaaagtttg tatttttggc agagttccgt
     9721 gctatctgcg tatcgagagt tgtcaaagat atcgtagagg gattcgaggc aaccaccgag
     9781 gattttcccc tcgaactggg cacttccttg caacaagtca aaacctgtat ttgtatgact
     9841 gacacgaggt gttcccaggg ccgtgggact aaaatcagtt cgttcctcat accaaacgtc
     9901 actagggcgg atttctgaaa ttcttcccgt ctcaatcaat tctttaaagt agtgaaggct
     9961 ataggctagc atttctttgt ctaattcaca aatgtctgct aaaaaggatt gaccataaaa
    10021 agtcttgatt cctaatttat gcaacatgag gtggttcatg gttgtatccg agaagccaag
    10081 aaaaattttt tgtttgataa ccttttggag ttggtcattt tcaaaaagat aaggtagcaa
    10141 gcgataggta tcgtctccac cgatggcaca taggatcatg tcgatgctat catcagaaaa
    10201 ggcatgaatc aaatcctctg cacgagcttc aggatggtcc ttgataaagt ctaatccttt
    10261 taacgaatgg ggcaaaaaga tgggattgag tcccagatcc ttgagacgtt ggatacccaa
    10321 gtccacttcg tgtttgacaa aatcctctcc gataatgcca ctagataaac taacaatacc
    10381 aatagtagaa accatatctc atcctcctag aaatagattg agcctatttt atcacaaaat
    10441 ataagagaaa gctatgtggg atttcagaag aatactttta atactcttcg aaaatctctt
    10501 caaaccacgt cagttttatc tgcaacctca aagctgtgct ttgagcaacc tgcagctagc
    10561 ttcctagttt gctctttgat tttcattgag tataactcaa aaaagtaagt gaaaaagcaa
    10621 ccgcacctgc agttgctttt atcgttttct tattcttgat agcgtgttgg tcctgcactt
    10681 gcactacgga tattcaagcg tttcttgaga tagaagcgaa gggctaggag ggctgctcca
    10741 ataatagcta gtggcaatgg atccagtact gggttaaggc tagctggtag gaagcttgtt
    10801 gcaaagaaga caagcaacca aaggaacata gaagctagga taactagtac agatttccag
    10861 aaaggtggac gttgactgcg atccatatct ggtccatagt attggtaaac aaagtagtac
    10921 atcaagtaga aggcaaatcc accaaccagt ccaactaata gaagagtaat caatccatag
    10981 ccgaaagctt gatctgccgc aaagaaggtt gtgagggcgc tgacaagggc aaagaggcta
    11041 gtgatgaaaa gagctgagtc cataatcatc agttttggat catcattttc ttttggatgc
    11101 tctttttcgt actgctcttt gacagtgaag ctatgagccc aatgagttgg tgcgccgtat
    11161 agggaacggg cagttgtacc tttagattgc tcctcaagga tgtggggaat gacttcctca
    11221 aaaatagtct ggatttcagc gtctgttttc ccatctttga tgaattgttg ggtagcaatg
    11281 tggacaaact cttggttttt cttagttaat ttttgtagat caatttgaga cataggaact
    11341 cctcttagaa ccatttttta tggatgagat agagagtgag cgagacactc atagcaaagg
    11401 cgataaagac gattaaccag aaggcatttg gctctccgtt tagggggatt tcattatcct
    11461 taaagttcat cccgtaggca gaaaagacca tggttgggat ggacatgacg atggtcacaa
    11521 gggccaaggt tttcatgatg ttgttctggt tgttagaaat gatagaggca aaggtctctg
    11581 tcatagaatg caagacgttt ccataaatat ctgccatctc gatggcctgt tgggtttcaa
    11641 tcagggtgtc ttcaagcagg tcttcgtcct caaggtattt cttgatattg ctggttgaac
    11701 tggtcaattt cttaatcacg cgctcatttg ttttgaggga ggccttgaaa tagacgatag
    11761 ttttttccaa ttccatgagc tcaatcaatt cttcattacg agttgattga tgcagttgac
    11821 tttcgatttg ttcactcttg cggtcgattg aacgaagggc tgttaggtaa agctctgcat
    11881 tgcgataaag aatttgaaag ataaaacgtg aacgcatgaa ggtatagaaa ttacgcaatc
    11941 gacggttgat aaagacatca aggacaggta gtggttccaa acacgtagtg ataatggttt
    12001 cctcagtgat gataatacca agcgggatgg ttacgtagta ggtgcggtta tttctttcct
    12061 ccgtgaccgg cacgtctacg ataatcaggg tatactcgtc ttcaatggta atacgagaca
    12121 tttcttccgc atcgagcggt gctcgaaggt cggcaatatc aatatcgaag gtgttggcga
    12181 tttcgagtga ttcattttga gtcggattga cgagattgat ccaagtaccc ggttcaagcg
    12241 tatcgatctc tttaaattca gttgttgtag agagaaaaac ttgtttcata tcccttatcc
    12301 tttctcattt ttcagatttt ttcacactgt actattatac tacaaaatcg gccttttggg
    12361 taatagaatc tcactttttt tggtataatg gtaagcaata atggactaga aagaacaaag
    12421 atgcaagata aaattgtcat tcatggggcg cgtgcccata atttaaaaaa tattgatgtg
    12481 gagattccgc gagacaagtt ggttgtcgtg actggcttgt caggttcagg gaagtccagt
    12541 ctggcttttg ataccctcta tgcggaggga caacgtcgct atgtagagag tttgtccgcc
    12601 tacgctcgtc agttcttggg aaatatggaa aaaccagatg tagatgccat tgatggtctc
    12661 agcccagcta tttccatcga ccagaaaacg actagtaaaa atcctcgctc gacggtggga
    12721 acgacgactg aaatcaatga ctatctgcgt ctcctctacg cacgtgtggg gacgccttac
    12781 tgtatcaacg gacatggagc tatcaatgct tcttctgtgg agcaaatcgt tgataaggtt
    12841 ttggagttac ctgaacgcca gcgcttgcag atcttggctc ctgtcatccg caagaaaaaa
    12901 ggccaacata agaatgttat cgagaaggtt cagaaagacg gatatgttcg tgtccgtgtg
    12961 gatggggaag tctatgatgt gaccgaagtg ccagagttgt ctaagagcaa gcaacacaat
    13021 atcgatgtcg tggttgaccg tattgttatc aaggagggca ttcgtagccg tctctttgat
    13081 tccattgaag ctgcccttcg tatcgcagaa ggttatgtga ttatcgacac catggacgat
    13141 tcggagttgc tcttctctga gcattatgcc tgtccagttt gtggatttac tgtcccagag
    13201 ttagagcctc gtctcttctc tttcaatgct ccctttggct cttgtagtga gtgtgacggc
    13261 ttggggatta agctggaggt ggatactgat ttggtagtgc cagatgccag caaaacgcta
    13321 cgtgaggggg ctttggcacc ttggaatcct atctcatcta actactatcc aaatatgcta
    13381 gagcaggcta tgacagcctt tggagtggat atggataagc cctttgagga cttgtcagaa
    13441 gaagataaga acttgattct ctacggctcg gatggcaaag aatttcattt tcactatgag
    13501 aatgaatttg gcggtgtgcg cgatattgac attccttttg agggagtcgt cactaatatc
    13561 aagcgtcgtt accatgagac taacagtgat tacacgcgca ctcagatgcg tctctacatg
    13621 aatgagctga cctgcggaac ctgtcaaggc tatcgtctca atgaccaggc cttgtctgtc
    13681 cgtgtgggcg gccagcaagg gccacatatt ggagaaatct cagacctgtc tatcgctgac
    13741 cacttggact tggtgagcca gttaactttg tctgaaaatg aagccatcat tgctcgtccc
    13801 attctcaagg aaatcaagga tcgtttgacc ttccttaata atgtgggtct taactatcta
    13861 accctgtcac gttcagcagg aaccctttca ggtggggaaa gtcagcgcat tcgtttggca
    13921 acccagattg gttccaacct atcaggtgtc ctctatatcc tagacgagcc gtcaatcggt
    13981 cttcaccaga gggacaatga tcgcctgatt gccagtctga aaaagatgcg tgacttgggc
    14041 aatactctta tcgtggtgga acacgacgaa gataccatgc gtgaggctga ttatctgatt
    14101 gacgttggtc ctggtgccgg tgtttttggt ggtgagattg ttgcagcagg tacacctaag
    14161 caagtggctc gcaacagcaa gtctatcaca ggtcagtact tgtcaggcaa acgtgtcatc
    14221 ccagtaccag aagagcgccg tgtcggaaat ggtcgtttta ttgaagtgat aggagcgcgt
    14281 gagaacaact tgcaaaatgt cacggctcgc tttccactag gaaaatttat cgcagtgaca
    14341 ggtgtgtcgg gttcagggaa atcgactcta atcaacagca ttctcaaaaa agccattgcc
    14401 cagaagctca accgtaattc agacaaacct ggtaagttta agactattac agggattgag
    14461 catgtcgacc gtttgattga tattgaccag agccctatcg gacggacgcc gaggtctaac
    14521 ccagctacct atacaggagt ttttgacgat atacgtgacc tttttgctca gacaaatgaa
    14581 gccaagattc gaggctacaa gaaggggcgc ttcagtttca acgtcaaggg aggtcgctgt
    14641 gaagcttgct caggtgacgg gattatcaag attgagatgc acttcttgcc agatgtttat
    14701 gtggcttgtg aagtttgcca cggaacccgc tataacagtg aaaccctaga agttcactac
    14761 aaggaaaaga atatttcgca ggtcttggat atgacggtca acgatgcggt agaatttttc
    14821 caacacattc cgaaaattca acgcaaactc cagaccatca aggatgtagg gctaggctat
    14881 gtgaccttgg gacagccagc taccactctt tctgggggag aagcccaacg tatgaagtta
    14941 gctagcgaac tccacaaacg ctcgacagga aaatctttct acattctgga tgagccgacg
    15001 acagggcttc atacagagga cattgctcgc ctgctcaagg ttttagctcg ctttgtagac
    15061 gatggcaata cagtcctcgt catcgagcac aatctggatg ttatcaagac ggcagaccat
    15121 atcattgact tgggacctga gggcggtgtc ggtggtggaa ccatcatcgt aacaggaact
    15181 ccagaagaag tagctgccaa cgaagccagc tatacaggac actatttgaa aggaaagtta
    15241 catcatgaat aaacgcgtac aagcatttct agctaaaatg caagaaaaag aactagatgg
    15301 tatcatcatc aacaatctta aaaacgtcta ttatttgact ggtttttggg gctcaaacgg
    15361 aacagtcttt atcagccgtg accgtcaggt cttagtgaca gactctcgct atatcatcgc
    15421 agctaagcaa gaaaccagtg gttttgagat tgtggctgat cgtgatgaat tggctgtcat
    15481 tgcaggaatt gttaaggaca tgggcttgac tcgtatcggt tttgaagatg agatttcagt
    15541 gtcttattac caccgtatgc aggcagcttt tgcaggtttg aacttgcttc cacaaactca
    15601 gtttgtggaa ggtcttcgta tgattaagga tgaggcagag attgcagcga ttcgcaaggc
    15661 ttgttctatc tcagaccaag ctttccgcga tgcgcttgac tttatcaaac caggaaaaac
    15721 tgaaattgag attgccaact tccttgattt ccgcatgcgt gagttgggag catctggctt
    15781 atcttttgat acgatcctag ctagcggtat caattcttct aaaccccatg cccatccaat
    15841 gcacaaacca gtggagttgg gagaagccat taccatggac ttcggctgtc tctatgacca
    15901 ctatgtcagt gatatgaccc ggactatcta tctagggcat gttagcgatg agcaggcaga
    15961 gatttacaat acggttctaa aagctaacca agccttgatt gaccaagcta aggcaggctt
    16021 aggtttccgt gactttgaca aaatccctcg tgatattatc attgaggcag gttatggtga
    16081 ctactttact cacggcattg gccacggtat tggtctggat atccatgagg aaccctactt
    16141 tagtcagact tctacagaaa ctattaagac aggtatggcc ttgaccgatg aaccaggtat
    16201 ctatatcgaa ggcaaatatg gcgttcgtat cgaggatgat atcctgatta cagagacagg
    16261 ttgtgaatta ttgaccctag ctccaaaaga gttgatagtc atttagttgt ttgtcaagaa
    16321 aaaagtacga aaatgacgaa aaaagtcaaa aaaaattaaa aataggtcgc aagtcggatg
    16381 ttttttatgg tataatagac taaactgata gtaacatgta gcgaaagggg taggtacatg
    16441 attaaaattt atacagtctc aagttgtact agctgtaaaa aagcaaaaac ctggctcaat
    16501 gcccaccagt taagttataa agaacaaaac cttggtaaag aaggaattac gagagaagaa
    16561 ttactggata ttctaaccaa aacagataac ggaatagcca gcatcgtttc gtctaaaaat
    16621 cgctatgcca aagcccttgg agtggatatt gaagatttga gtgtcaatga agttctcaat
    16681 ctgattatgg aaacaccgag aattttaaag agcccaatcc ttgtagatga aaaacgcctg
    16741 caagttggct acaaggaaga cgatattcgt gccttcctac cacgctctgt ccgtaatgta
    16801 gaaaatgcag aagcacgttt gcgtgcagct ctataaacat caaggctggg agcacttccc
    16861 agtcttattc tattttaatt tcaaaaagaa agaagaaaga aatgaaaaaa atagttcttg
    16921 ttagtctagc tttccttttt gtcctggttg gttgcggaca gaaaaaagaa actggaccag
    16981 ctacaaaaac agaaaaagat acgcttcagt cggcattgcc agttattgaa aatgctgaga
    17041 agaatacagt tgtaaccaag actttggtct tgcccaagtc agatgatggt agccagcaga
    17101 cacaaacaat tacttacaaa gacaagactt ttttgagtct aactatccaa caaaaacgtc
    17161 cagtctctga tgagttgaag acttatattg accaacatgg agtggaggaa actcaaaaag
    17221 ctcttcttga agcggaggag aaggataagt ctatcattga agctcgtaaa ttggcaggtt
    17281 tcaaacttga aacaaaacta ttgagcgcaa cggaacttca aacaacgact agttttgatt
    17341 ttcaagttct ggatgtcaag aaggcttccc agttggaaca tctgaagaat attggtttgg
    17401 aaaatctttt gaaaaatgaa ccaagcaaat atatttcaga tagattggca aatggcgcga
    17461 cagaacaata gaaaatccaa ataaatagac tgcctgaatt gtagaaggta aggaattatg
    17521 ctataatggt actattctaa ggaaagaggg tgttagaatg ggatttactg aagaaacagt
    17581 acgttttaaa ttggacgatt ccaataaaaa agaaattagc gaaactttga cagatgttta
    17641 tgcttcgttg aacgataagg gttacaaccc aattaaccaa atcgtaggtt acgtattgag
    17701 tggagaccct gcctacgttc ctcgttataa taatgcacga aatcaaatcc gtaagtatga
    17761 gcgtgatgaa atcgttgagg aattggttcg ctactacctc aaaggacaag gagtcgatct
    17821 ataacctatg agaattatgg gattggacgt cggttcaaaa acggtagggg tggcgattag
    17881 cgatccgctt ggttttacag ctcaagggct tgaaatcatc cagataaatg aagaacaagg
    17941 ccaatttggt tttgaccgcg ttaaggaatt ggttgatact tacaaggtgg aacgatttgt
    18001 agtgggcttg cctaaaaaca tgaacaatac aagtggaccg cgcgtagaag ctagtcaagc
    18061 ctacggagca aagctagaag agttttttgg tttaccagta gactatcagg atgaacgctt
    18121 gacaacagtg gctgctgagc gcatgttgat tgaacaagca gatatcagtc gcaataagcg
    18181 caagaaagtc attgataagt tagcagctca gctgatttta caaaattatt tagatagaaa
    18241 attttaatat aaaggagagg ctatgtcaca cgatcataac cacgaccacg aagaacgtga
    18301 actaatcaca ctagtagatg agcaaggaaa tgaaaccttg tttgaaatcc ttttgacaat
    18361 tgatggaaaa gaagaatttg gtaaaaacta tgttcttcta gtaccagtta acgcagaaga
    18421 agacgaagac ggacaagttg aaatccaagc ttactcattc atcgaaaacg aagatggaac
    18481 agaaggcgaa ttgcaaccaa tcccagaaga ctcagaagac gaatggaaca tgattgaaga
    18541 agtcttcaat agttttatgg aggagtaaaa atgttcagtg aatattttta ccccagttcc
    18601 tttaaataag agagaactgg taagtccggg ggacgttttt agcccagctc cttgaagtaa
    18661 gagagagctg gtaagtccgg ggacgttttt agccctgctt ttagaaaaaa gaaagagcag
    18721 gcagtctggg agactgtatc agcccaactc ccagaaatgg ggaagagttg gaacatccag
    18781 tggatgtttt tagcccagtt cctttaaata agagagaact ggtaagtccg ggggacgttt
    18841 ttagcctgac gctaaaaata aaaacagtca gtaagttcgg gggatgtttt agcccagctc
    18901 cttgaaataa gatagagctg gtaagtctga gaaactgtat cagcccaact cccagaaatg
    18961 gggaagagtt ggaacgtcca gtgaacattt ttaccccaac tcttagaaat tgagaagagt
    19021 tggaacatct agtggatgtt tttagcccat attcacattc atagtagcta gttattagct
    19081 aaccaggtaa gaggtcggga cgaaaatcct ggccttgttt ttattagtaa ccatacttta
    19141 atgcgataat tgattggact tttgtcaagg agatgtgtat gtttgaagta gaagaatggc
    19201 tccatagtcg gattggtttg aattttcgat caggtttggg ccgaatacag caagcggtgg
    19261 atttgttagg aaatcctgag cagtcttacc ctattatcca cgtaacaggg actaatggaa
    19321 aaggatctac cattgctttt atgagggaat tatttatggg gcatggcaaa aaagttgcga
    19381 cctttacctc ccctcatatc gtctctatca atgaccgaat ctgcattaat gggcaaccta
    19441 tagcagacgc agactttatc cgtttggctg atcaggtcaa ggagatggag aaaacgcttc
    19501 tgcaaactcc tgaccagttg tccttttttg aattgctgac cttagttgct tttctttatt
    19561 ttagggagca agaggtggat ttggttttat tagaagtggg aattggtggc ttacttgaca
    19621 cgaccaatgt ggtaactgga gagcttgctg tcatcacctc cattgggctt gaccatcaag
    19681 aaaccttggg tgatagtcta gaagcaattg cagagcagaa agctggtatt ttcaaggctg
    19741 gtaagaaggc agtgattgcg aaattgcctc cagaagctag gcttgtctgt cagaaaaaag
    19801 ccgaatcttt agctgttaac ctttatcagg caggtcaaga ttttttaatg ctgaatggtg
    19861 atttttcaag ctctttacta aatctttcac agctgaacat aggcttagaa ggagtctatc
    19921 agcaggagaa tgcagccttg gcgttgcaaa cttttcttct ttttatggga gaaggaaagg
    19981 aagctgttga tgaacaggct gtaagaaagg ccttggaaca gacccattgg gctggtcgct
    20041 tggagcgtat tcgcccacag atttacttgg atggtgctca taacctccct gccttgactc
    20101 gcttggttga gtttatcaaa gaaaaagagc aggaaggtta tcgacctcaa atcctctttg
    20161 gatccttgaa acgtaaggat tatcaaggga tgttgggtta tctgactgaa aatttacctc
    20221 aagtggaact caaaatgact ggctttgact atcaagggtc tttggacgag acagatgtaa
    20281 caggttacca tgtaattccc tcttaccgag aatttatcag cattttcgaa gaaagggcag
    20341 acgctcaaga cttgctgttc gttacagggt ctctctattt tatctcagaa gtacggggct
    20401 acctgctgga ccgtgagcag ataaattgac ccccttgttt gaacttgtta tactagggga
    20461 attgctggtt agagaaaatt tctctaaatt ggtagcagaa aggaaattca tcatgaaatt
    20521 aaaaagattc acactttctc ttgcttctct agcaagtttt agtctcttag tagcttgttc
    20581 acaaagagct caacaggttc aacagcctgc tcaacaacct gctcaacaga ataccaatac
    20641 tgcaaatgca ggaggtaacc aaaatcaagc ggctccagta caaaaccaac ctgttgctca
    20701 accgaccgat attgatggga cttatactgg tcaggatgac ggagaccgta tcactttagt
    20761 ggtaactgga acgactggta catggactga gctcgaatct gacggggatc agaaagtcaa
    20821 acaggttaca tttgattcag caaatcaacg catgattatt ggcgatgatg tcaaaattta
    20881 cactgtaaac ggtaatcaaa tcgtcgtaga tgatatggat agagacccat cggaccaaat
    20941 cgttttaact aaataagact atgcaagtag aaagggctgg atttttcagc ctttttactt
    21001 ttacctagaa aataatcata aatacttgcc ttctaccgaa tacctgcgtt atactagtat
    21061 caattacttg tttttagcat tcaaactatc aaggagggga tatgaaatat aggaaatttc
    21121 aattattgat gtccaagtat ggctttagtc tttcgattat gctacttgaa ctttgtcttg
    21181 tttttggtct ctttctttat ttaggacgca tggctcccat tttatggatt actgtcctca
    21241 ttctactgag tatcatcaca atcatttcga tagtcaaccg taatacgact cctgagaata
    21301 aggtaacctg gttgttagta gcctttgtgc cagtatttgg tcccttgctc tatctgatgt
    21361 ttggtgaaag gcgattgtcc aaaaaagaaa tcaaacaact gaagaagcta ggctctatgc
    21421 atttccaaga agcaaatagc cagctactaa aagagaaatt aaaagaaagt gacaaggcag
    21481 cttatggagt catcaagtcc ttattgagta tggataccaa tgctgacatc tatgatcaaa
    21541 ctgcctctac attttttcct aacggagaag ctatgtggaa aaagatggta gaagatctta
    21601 aaaaggctga gaaatttatt ttcttggaat attacattat agaagaaggt ttgatgtgga
    21661 atcgcatact agatatacta gagcaaaagg tagctcaggg tgtagaggtt aagatgctct
    21721 atgatgatat cggctgtatg gctactttaa caggagatta tgcacatcga cttcgtcagc
    21781 tgggcatcga ggcccataaa ttcaataaag ttattcctcg tctgacagtg gcttataata
    21841 acagagatca tagaaaaata ttgattgttg atggtcagat agcctatact ggtggggtca
    21901 atctggcaga tgagtacatt aaccacgtcg agagatttgg ttattggaag gatagtggaa
    21961 ttcgcttaga cggactagca gtaaaagccc tgacacgctt atttttgacc acttggtaca
    22021 ttaatcgagg agaaattagt gattttgatc aatatcattt agaaaatcat tctatcccga
    22081 gtgacggttt aaccattcca tacggaagtg gacccaagcc aatttttcga gcgcaggtag
    22141 ggaaaaaagt ttatcagagt ttaatcaatc aagcaacaga atcggtctat attacgacac
    22201 cttatttgat tatagattat gatttaacag agacaatcaa aaatgcagct atgagagggg
    22261 tcgatgttcg aattatcacc ccttacatac cagataagaa gttcattcag ttagtcacga
    22321 gaggagctta tcccgacctt ctttctgctg gtgttcggat ttatgagtat agtccaggtt
    22381 ttattcatag taagcagatg ttggtagacg aagattttgc ggtggtgggg acaatcaatc
    22441 tcgactaccg gagcttggta caccattatg aaaatgcagt cttactctat aaaactcctt
    22501 ctataaggga aatcgcccga gattttcgaa atatatttgc agattctcag gaagtctatc
    22561 ctcattctat caaaacgagc tggtatcaaa agcttgtaaa agaaatcgcc cagctattcg
    22621 cccctatctt ataagaaatc tagaacttag gatacaaacc agacctattt ttcttgtctt
    22681 ttacgaatag aaagaaagag gaggggaatt catgctaact aaggaagaag tgaatgatat
    22741 gattgagttc aaacagacac atttacaccg ctttcgggaa attgaaacct ttgtgaaact
    22801 atgcaaaaaa tcactcaaac agccatcgca agctgacaat cctaggactt tttgatatac
    22861 taagacagat aaattgaaca ggagaaacga tatgagtgtg tatggtagag tagaagaagt
    22921 tcataaggag aatcgtgaac ctctagaata ccaaatcgaa caagaatcgc atcatcgtga
    22981 atcaagtcgt cttcctttgg tgaaaatttt actatggagt acgcttgtaa cggggataac
    23041 tctaggagta ccgctattac tcgacttaat gagtgcacaa gaagtgcagg atttttatgc
    23101 aggttgggcg cttcatcaga cagggaagat ttacagcgac tattatggaa gtcaaggttt
    23161 gctttattat ttgctgactt acgtgagtca gggcggattt ttctttgcca tctttgagtg
    23221 gttagccttg gtagcaggag gatttttcct ttttagatca gcggacacct tgacagagca
    23281 aggagaccaa gctggacatc tggtgactat tttttacatg ctagttacag gtcttgcttt
    23341 tggtggaggc tatgcgactc ttttagcgct tcctttctta ttcgcagcct ttagtttagt
    23401 tgcggcttac ctaagcaatc caagccatga taagggattt gtacggattg ggctagcttt
    23461 ggcaggcgga tttttctttg ctcccttatc atcgctcctg tttattgctg tagtgagttt
    23521 aggcttgttg gtctttaacc ttgggcatag acgctttgcg catgggtttt atcagtttct
    23581 tgcagtggct ttaggttttt cacttgtctt ttatccaact gcctactata gtgctgcaac
    23641 aggaagtttt ggggatgcga ttagtggtat tcgttatcct attgacagta ttcgctttga
    23701 ttttacttct aaaattttag agaatatgtt tttttatggc ttgttgtccc ttggtttggg
    23761 atttgtggtt tgtatctttt taggtctctt tcaatccaaa ccctttaaac tatacgtcat
    23821 ttcggttcct gcaagtcttg tggtaatttt aggtttgatt ttacttttct tttcacaaga
    23881 gcctctgcac gcttcttatt tgatggtcgt cttccctgtt ttcctacttt tattggtaac
    23941 caatattaag agtcaacaga gggggcgtag tgctagaaga agcctaagag aaacgccagt
    24001 tagcctatgg agtcgtttct tcaaaggaaa tctatatctg ctagtttttg ggtttgtcta
    24061 tcttttgtct gttccttttt tgatgaagtt tgtcctttat ccagtacctt atcaagaacg
    24121 taatcgtctt gctgatttgg taaaagagga gacaaatacg gaagatgcta tctatgcatg
    24181 ggatgatact gcgactcttt atcgtaagag tgagcgcttg tcgccatcgg cgattttgtc
    24241 cccgttgcac tatacagcaa ctgaggaaaa tcgtaataag ttacttaatg acttgaaaga
    24301 aaaacaacct aaggtgattg tggtaaatga caaggtggta gtctggtctg aagtggaaac
    24361 acttttaaaa gaaaattacc aacaagtaaa gactgattac tcagagttta aagtctataa
    24421 aatt
//
LOCUS       DAVMGZ010000015        23841 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000015 DAVMGZ010000000
VERSION     DAVMGZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23841
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.016"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            337..2490
                     /locus_tag="VYQ93_000516"
     CDS             337..2490
                     /locus_tag="VYQ93_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV3335256.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2503..3852
                     /locus_tag="VYQ93_000517"
     CDS             2503..3852
                     /locus_tag="VYQ93_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV3335257.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSFYQSYK
                     SQGEENPQTKVQVVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4022..4729
                     /locus_tag="VYQ93_000518"
     CDS             4022..4729
                     /locus_tag="VYQ93_000518"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV3335258.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            4786..8511
                     /locus_tag="VYQ93_000519"
     CDS             4786..8511
                     /locus_tag="VYQ93_000519"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV3335259.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSTADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8604..10046
                     /locus_tag="VYQ93_000520"
     CDS             8604..10046
                     /locus_tag="VYQ93_000520"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV3335260.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNTASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10083..11105
                     /locus_tag="VYQ93_000521"
     CDS             10083..11105
                     /locus_tag="VYQ93_000521"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV3335261.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKTLEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11102..11647
                     /gene="purN"
                     /locus_tag="VYQ93_000522"
     CDS             11102..11647
                     /gene="purN"
                     /locus_tag="VYQ93_000522"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV3335262.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11731..12240
                     /locus_tag="VYQ93_000523"
     CDS             11731..12240
                     /locus_tag="VYQ93_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV3335263.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEY
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12265..13812
                     /gene="purH"
                     /locus_tag="VYQ93_000524"
     CDS             12265..13812
                     /gene="purH"
                     /locus_tag="VYQ93_000524"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV3335264.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLGSDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            13934..15196
                     /gene="purD"
                     /locus_tag="VYQ93_000525"
     CDS             13934..15196
                     /gene="purD"
                     /locus_tag="VYQ93_000525"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV3335265.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHYKLIDFAKANDIAWTFIGPDDALAAGIVDDFHAAGLKAFGPTRLAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAADTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            15598..16086
                     /gene="purE"
                     /locus_tag="VYQ93_000526"
     CDS             15598..16086
                     /gene="purE"
                     /locus_tag="VYQ93_000526"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV3335266.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEAYSRGIKVIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16073..17164
                     /gene="purK"
                     /locus_tag="VYQ93_000527"
     CDS             16073..17164
                     /gene="purK"
                     /locus_tag="VYQ93_000527"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV3335267.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPVSRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEVD
                     LEAAYALAESADCVLEEFVNFALEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVAENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17174..17401
                     /locus_tag="VYQ93_000528"
     CDS             17174..17401
                     /locus_tag="VYQ93_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV3335268.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17464..18762
                     /gene="purB"
                     /locus_tag="VYQ93_000529"
     CDS             17464..18762
                     /gene="purB"
                     /locus_tag="VYQ93_000529"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV3335269.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(18817..22743)
                     /gene="strH"
                     /locus_tag="VYQ93_000530"
     CDS             complement(18817..22743)
                     /gene="strH"
                     /locus_tag="VYQ93_000530"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV3335270.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDKAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQEEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEILSQPAPAEESK
                     ALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(23056..23772)
                     /locus_tag="VYQ93_000531"
     CDS             complement(23056..23772)
                     /locus_tag="VYQ93_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV3335271.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT         6469 a         4751 c         5676 g         6945 t
ORIGIN      
        1 atttcggttt tgttaacttg taattcttat attttctagg tagaacgttt gttctgccta
       61 tttttttatc caaaaagtgc agttgggagg gagataggct catttgggaa ggaagtccag
      121 tttttgttta gtgattgggg taagatagtt gttatcagat gagtttatac tcttcgaaaa
      181 tcaaattcaa accacgtcaa cgtcgccttg ccgtatatat gtgactgact tcgtcagtcc
      241 tatctacaac ctcaaaacag tgttttgagc agcctgcggc tagtttccta gtttgctctt
      301 tgattttcat tgagtattag ggaaaaggag atgaatatga aatttgggaa acgtcactat
      361 cgtccgcaag tggatcagat ggactgcggt gtagcttcat tagccatggt ttttggctac
      421 tatggtagtt attatttttt ggctcacttg cgagaattgg ctaagacgac catggatggg
      481 acgacggctt tgggcttggt caaggtggca gaggagattg gttttgagac gcgagccatt
      541 aaggcggata tgacgctttt tgacttgccg gatttgactt ttccttttgt tgcccatgtg
      601 cttaaggaag ggaaattgct ccactactat gtggtgacgg ggcaggataa ggatagcatt
      661 catattgccg atccagatcc cggggtgaag ttgactaaac tgccacgtga gcgttttgag
      721 gaagaatgga caggagtgac tctttttatg gcacctagtc cagactataa gcctcataag
      781 gaacaaaaaa atggtctgct ctcttttatc cctatattag tgaagcagcg tggcttgatt
      841 gccaatatcg ttttggcaac actcttggta accgtgatta acattgtggg ttcttattat
      901 ctgcagtcta tcattgatac ctatgtgcca gatcagatgc gttcgacact agggattatt
      961 tctattgggc tagtcatcgt ctacatcctc cagcaaatct tgtcttacgc tcaggagtat
     1021 ctcttgcttg ttttggggca acgcttgtcg attgacgtga ttttgtccta tatcaagcat
     1081 gtttttcacc tccctatgtc cttctttgcg acacgcagga caggggagat cgtgtctcgt
     1141 tttacagatg ctaacagtat catcgatgcg ctggcttcga ccatcctttc gattttccta
     1201 gatgtgtcaa cggttgtcat tatttccctt gttctatttt cacaaaatac caatctcttt
     1261 ttcatgactt tattggcgct tcctatctac acagtgatta tctttgcctt tatgaagccg
     1321 tttgaaaaga tgaatcggga taccatggaa gccaatgcgg ttctgtcttc ttctatcatt
     1381 gaggacatca acggtattga gactatcaag tccttgacca gtgaaagtca gcgttaccaa
     1441 aaaattgaca aggaatttgt ggattatctg aagaaatcct ttacctatag tcgagcagag
     1501 agtcagcaaa aggctctgaa aaaggttgcc catctcttgc ttaatgtcgg cattctctgg
     1561 atgggggctg ttctggtcat ggatggcaag atgagtttgg ggcagttgat tacctataat
     1621 accttgctgg tttactttac caatcctttg gaaaatatca tcaatctgca aaccaagctt
     1681 cagacagcgc aggttgccaa taaccgtcta aatgaagtgt atctagtagc ttctgagttt
     1741 gaggagaaga aaacagttga ggatttgagc ttgatgaagg gagatatgac cttcaagcag
     1801 gttcattaca agtatggcta tggtcgagac gtcttgtcgg atatcaattt aaccgttccc
     1861 caagggtcta aggtggcttt tgtggggatt tcagggtcag gtaagacgac tttggccaag
     1921 atgatggtta atttttacga cccaagtcaa ggggagatta gtctgggtgg tgtcaatctc
     1981 aatcagattg ataaaaaagc cctgcgccag tacatcaact atctgcctca acagccctat
     2041 gtctttaacg gaacgatttt ggagaatctt cttttgggag ccaaggaggg gacgacacag
     2101 gaagatatct tacgggcggt cgaattggca gagattcgag aggatatcga gcgcatgcca
     2161 ctgaattatc agacagaatt gacttcggat ggggcaggga tttcaggtgg tcaacgtcag
     2221 agaatcgctt tggcgcgtgc tctcttgaca gatgcgccgg tcttgatttt ggatgaggcg
     2281 actagcagtt tggatatttt gacagagaag cggattgtcg ataatctcat ggctttggac
     2341 aagaccttga ttttcattgc tcaccgcttg actattgctg agcggacaga gaaggtggtt
     2401 gtcttggatc agggcaagat tgtcgaagaa ggaaagcatg ctgatttgct tgcacaggga
     2461 ggcttttacg cccatttggt caatagctag aaagaggaga ggatgaaacc agaattttta
     2521 gaaagtgcgg agttttataa tcgtcgttac cataattttt ccagtagtgt gattgtaccc
     2581 atggcccttc tgctcgtgtt tttacttggc tttgcaactg ttgcagagaa ggagatgagt
     2641 ttgtccacta gagctactgt cgaacccagt cgtatccttg caaatatcca gtcaactagc
     2701 aacaatcgta ttcttgtcaa tcatttggaa gaaaataagc tggttaagaa gggggatctt
     2761 ttggttcaat accaagaagg ggcagagggt gtccaagcgg agtcctatgc cagtcagttg
     2821 gacatgctaa aggatcaaaa aaagcaattg gagtatctgc aaaagagcct gcaagaaggg
     2881 gagaaccact ttccagagga ggataagttt ggctaccaag ccacctttcg cgactacatc
     2941 agtcaagcag gcagtcttag ggctagtaca tcgcaacaaa atgagaccat cgcgtcccag
     3001 aatgcagcag ctagccaaac ccaagccgaa atcggcaacc tcatcagtca aacagaggct
     3061 aaaattcgcg attaccagac agctaagtca gctattgaaa caggtgcttc cttggccggt
     3121 cagaatctag cctactcttt ttatcagtcc tacaagtctc agggcgagga aaatccccaa
     3181 actaaggttc aggtagttgc acaggttgaa gcacagattt ctcagttaga atctagtctt
     3241 gctacttacc gtgtccagta tgcaggttca ggtacccagc aagcctatgc gtcagggtta
     3301 agcagtcaat tggaatccct taaatcccaa cacttggcaa aggttggtca ggaattgacc
     3361 cttctagccc agaaaatctt ggaggcagag tcaggtaaga aggtacaggg aaatctttta
     3421 gacaagggga aaattacggc gagtgaggat ggggtgcttc atcttaatcc tgagaccagt
     3481 gattctagca tggttgcaga aggtgcccta ctagcccaac tttatccatc tttggaaaga
     3541 gaaggtaaag ccaaactcac agcttatcta agttcaaaag atgtagcaag aatcaaggtc
     3601 ggtgattctg ttcgctatac tacgactcat gatgccggga atcaactttt cctagattct
     3661 actattacaa gtattgatgc gacagctact aagactgaga aagggaattt ctttaaaatc
     3721 gaggcggaga ctaatctaac ttcggagcag gctgaaaaac ttaggtacgg ggtggaaggc
     3781 cgcttgcaga tgattacggg caagaaaagt tatctacgtt attatttgga tcaatttttg
     3841 aacaaagagt aatgttcgtg tttttagagt taaataattt ttaaactgtg agaaagattc
     3901 ttcttgcagt tttttcttta caatttttgg aaaacatctg ctatttattc ggttaaattc
     3961 ttgtattttt tggttttttg tggtaaaatg tgctcaagta atacgaaagg cgaactttaa
     4021 aatgtcaaaa caattgatct attcgggaaa agctaaagat atctatacaa ctgaggatga
     4081 aaatcttatt atttcaactt acaaggacca ggcgactgct ttcaacggtg tcaagaagga
     4141 gcagattgca ggtaagggag tcttgaataa tcagatttca tcttttattt ttgagaaatt
     4201 aaatgcggct ggtgtggcga ctcactttgt ggagaaactt tcagacacgg aacaactcaa
     4261 taaaaaggtt aagattattc ctttggaagt cgtgctccgc aattatactg ctggttcctt
     4321 ttcaaaacgt tttggtgtgg atgagggaat cgccttggag actccgattg tcgaatttta
     4381 ctacaaaaat gatgatttgg atgatccatt tatcaatgat gagcatgtga aattcctaca
     4441 gattgcggat gaccagcaga ttgcctactt gaaggaagaa acgcgtcgta tcaatgaact
     4501 attgaaagtc tggtttgctg agattgggct taaattgatt gactttaagc tagagttcgg
     4561 ttttgacaag gatggcaaga ttatcttggc agacgaattt tcaccagata actgccgctt
     4621 gtgggatgct gatggcaacc acatggataa ggatgttttc cgtagaggat tgggagaact
     4681 aaccgacgtt tatgagattg tttgggaaaa gttgcagggt ttaaaataac aacctcaagg
     4741 ctgtttggga atattgcaag agctgaaata aaggaataag aattgatgga taaacgtatt
     4801 tttgttgaaa aaaaggctga ttttcaggtc aagtcagaga gtttggttag agagctccag
     4861 cacaacttgg gactgtcaag cttgaaaagt attcgtattg tgcaagtata tgatgtattt
     4921 gacttggttg aggacttgtt tgcacctgca gagaagcaca ttttctctga gcaggtaacc
     4981 gaccatgttt tagatgaagt atctgtgcag gcggatcttg ctaactatgc tttctttgcc
     5041 attgaaagtc taccagggca gtttgaccag cgtgcagctt cgtcacagga agccttgctt
     5101 ttgttgggaa gttcgagtga cgtgacagtc aacacagccc aactttactt ggtgaataaa
     5161 gatattgatg cgactgagtt ggaagctgtc aaaaactacc tgctcaatcc agttgattct
     5221 cgtttcaagg atatcacgat agggattgcc aagcaggagt tttcagagtc agacaagacc
     5281 attccaaaat tgcctttctt tgaaagctat acagcagaag actttgctcg ctacaaggcc
     5341 gaacaaggga tggccatgga agtggatgat ttgctcttta tccaagacta ctttaagtca
     5401 atcgggcgcg tgccaactga gactgaactc aaggttttgg acacttactg gtctgaccac
     5461 tgccgtcata cgacttttga gacagagttg aaacacatcg acttttcagc ttctaaattt
     5521 caaaagcaat tgcagtcaac ctatgacaag tatattgcca tgcgcgagga attaggtcgg
     5581 tctgaaaaac cacaaacctt gatggatatg gcgactattt tcggtcgtta tgagcgtgct
     5641 aatggacgat tggatgatat ggaagtctct gacgaaatca atgcctgctc agttgaaatt
     5701 gaagtggacg ttgatggtgt caaggaacct tggctcctca tgtttaaaaa cgaaacccac
     5761 aaccatccaa cagaaattga gccatttggt ggagcggcta cctgtatcgg tggagctatt
     5821 cgtgatccgt tgtcaggccg ttcctatgtt taccaagcca tgcgtatttc aggtgctggt
     5881 gatattacag caccgatttc ggaaactcgt gctgggaaat tgccacaaca agtcatttct
     5941 aaaacagcaa ctcatggtta ttcttcatat ggtaaccaga ttgggcttgc cacaacctac
     6001 gttcgtgaat acttccaccc aggctttgta gctaaacgta tggaacttgg tgccgttgtt
     6061 ggtgcggctc ccaagggcaa tgttgtccgt gaaaaacctg aagcaggtga tgtgatcatc
     6121 cttctcggag gtaaaacagg tcgtgatggt gtcggtggtg cgacgggctc ttctaaggtt
     6181 caaacagttg agtctgtaga gactgctggt gctgaggttc aaaaaggaaa tgccatcgaa
     6241 gaacgcaaga ttcagcgcct cttccgtaat ggcaatgtca ctcgtctgat caagaagtcc
     6301 aatgactttg gggcaggcgg tgtctgtgtg gctatcggtg aattggcaga cggtcttgaa
     6361 atcgacctca acaaggtgcc tcttaaatac cagggcttga atggtacaga aattgccatc
     6421 tctgaatcac aagaacggat ggcggtcgtg gttcgtcctg aagatgtgga tgccttcgtt
     6481 gccgaatgta acaaagaaaa tattgatgct gttgtggtgg cgacagtaac tgaaaaacca
     6541 aatcttgtca tgcactggaa tggtgagaca atcgttgact tggagcgtcg tttccttgac
     6601 accaatggtg tgcgcgtggt tgtcgatgcc aaagttgtgg acaaggatgt caaactccca
     6661 gaagagcgtc aaacatctgc tgaaacactg gaatcagata cccttacggt tctatctgac
     6721 ctcaaccatg caagtcaaaa aggattacag actgtctttg actgctctgt tggacgctca
     6781 acggttaatc acccacttgg tggtcgttac caactcacac caactgaggc atctgtgcag
     6841 aaattgccag ttcaacacgg tgtgactcat actgcgtcgg tcattgctca aggtttcaac
     6901 ccatatgtag ctgaatggtc tccataccac ggtgctgctt acgcggttat cgaagcaact
     6961 gctcgtttgg tggctgctgg tgccaactgg tctaaggctc gtttctctta ccaagaatat
     7021 ttcgagcgga tggacaagca agcagagcgt tttggtcagc cagtagccgc ccttctaggc
     7081 tctattgaag ctcagattca acttggcttg ccatctatcg gtggtaagga ctccatgtct
     7141 ggtacctttg aagaattgac cgttccgcca accttggttg cctttggggt gacgacggca
     7201 gatagccgta aggtgctctc tccagaattt aaagctgttg gggaaaatat ctactacatc
     7261 ccaggtcaag ccctctctgc agagattgat tttgacttga ttaagaaaaa ttttgctcag
     7321 tttgaagcaa gccaagctga ccataaagtg acatctgcat cagctgtcaa atacggtggt
     7381 gtagttgaaa gtttggctct tgctaccttt ggaaactata ttggtgcaga ggtgaccttg
     7441 cctgaactta aaacagcttt gacagctcaa ttaggcggct ttgtcttcac atctcctgaa
     7501 gaaattgctg gagtagagaa gattggacaa acgaaagcag actttacact gactgtcaac
     7561 ggtgtgaagc tagatggaca caagcttgac agtgcatttc aagggacatt ggaagaagtt
     7621 tacccaacag aatttaccca agcgaaagaa ctagaagaag taccagctgt ggcatcagat
     7681 gttgtgatta aagccaaaga aaaggttgaa aaacctgtgg tttacatccc agtatttcca
     7741 ggaaccaact cagaatatga ttcagctaag gccttcgaaa aagaaggtgc agaggtcaat
     7801 ttggtgccat tcgtgacctt gaatgaagaa gctattgtca agtcagttga aactatggtt
     7861 gacaatatcg acaagactaa tattctcttc tttgccggtg gattctcgac tgcggatgaa
     7921 ccagatggtt cagctaagtt tatcgtcaat atcctgctta atgaaaaagt gcgtgtggct
     7981 attgatagct ttatcgcccg tggtggcttg attatcggta tttgtaatgg attccaagcc
     8041 ttagtcaaat cgggtctcct accatacgga aactttgaag atgctaacag tactagtcca
     8101 accctcttct acaatgatgc caaccaacac gtggccaaga tggtggaaac tcgcattgcc
     8161 aataccaact caccatggtt ggctggtgtg caagtgggcg atatccacgc tattcctgtt
     8221 tcgcacggtg aagggaagtt tgtcgtgacg gctgaggaat ttgcagagct ccgtgacaat
     8281 ggacaaattt tcagccaata cgttgacttt aacggtaaac caagtatgga ttctaagtac
     8341 aatccgaatg gttctgtcca tgccatcgaa ggaattacca gcaagaatgg tcaaatcatc
     8401 ggtaagatgg gccactcaga acgttatgag gatggtcttt tccaaaatat cccaggcaat
     8461 aaagaccaac acctgttcgc atcagcggtt aaacatttca ctggaaaata agacttacag
     8521 attttctaat agatagtatc agtaatgtaa aagtcatgta aatctagctc ttgatgatta
     8581 caaatgaaaa ttaggtataa aaaatgacat acgaagtaaa atctcttaat gaagaatgtg
     8641 gtgttttcgg tatttgggga catccagatg ctgctaagtt gacctatttt ggactccaca
     8701 gtcttcaaca ccgtggtcag gagggggcag gaatcctctc caacgatcaa ggacaactga
     8761 agcgccatcg tgacatgggg cttttatcag aagttttcag aaatccagct aatttggata
     8821 aattgacagg agctggtgcg attgggcatg tgcgttatgc gactgctggc gaagcttctg
     8881 tagataacat ccagcccttc ctcttccgtt ttcacgatat gcagtttggc ttggctcata
     8941 atggaaatct gaccaataca gcctctctca agaaagaact ggaacaaaga ggagcaattt
     9001 tcagcgcgac ttcggactct gaaatcttgg ctcacctcat tcgtcgcagt cataatccta
     9061 gcctgatggg caaaatcaag gaagcgctca gccttgtcaa aggtggtttt gcctatatct
     9121 tgctgtttga ggacaagttg attgcggctc ttgaccctaa tggctttcgt ccgctttcta
     9181 tcgggaaaat ggccaacgga gcggtggttg tttcctctga aacctgtgct tttgaggtca
     9241 ttggtgccga gtggattcgt gatttgaagc caggtgagat tgtgatcatt gatgacgagg
     9301 gcattcagta tgacagctat acagatgata cccagttggc gatttgttct atggagtata
     9361 tctactttgc ccgccctgat tctaatatcc acggtgtcaa tgtccatacg gcacgtaaga
     9421 gaatgggagc gcaattggcg cgagaattta agcatgaggc agatattgta gttggtgtgc
     9481 ccaattcttc cctaagcgcg gctatgggat ttgcggaaga atcaggctta ccaaatgaaa
     9541 tgggtctgat caaaaaccaa tacacccagc gaacttttat ccaaccgact caagaattgc
     9601 gggagcaagg agtgcggatg aaactgtctg ctgtttcggg tgttgtcaaa ggcaaacgtg
     9661 tggtcatggt ggatgattcc attgtacgtg gaacaacctc tcgtcgtatc gttcagctct
     9721 tgaaagaagc gggtgcgact gaggttcacg ttgccattgg aagtcctgca ctagcgtatc
     9781 catgtttcta cgggattgat atccagaccc gtcaggagct gattgcagcc aatcatacgg
     9841 tcgaagaaac tcgccaaatc attggtgcgg acagtctgac ttatctttca attgatggct
     9901 tgattgagtc gattggtatc gaaacagatg cgccgaacgg tggtctctgt gtcgcttact
     9961 ttgacggtga ctacccaacg cctctttatg actacgaaga agactatcgt agaagtttgg
    10021 aagaaaagac cagtttttac aagtaggcga cagattctcc attaaagaaa aggaaaaaac
    10081 aaatgacaaa taaaaatgca tatgcccagt cgggtgtgga tgttgaagcg ggttatgaag
    10141 ttgttgaacg gattaaaaag cacgtggccc gtacggagcg tgcaggtgtc atgggagctc
    10201 ttggtggctt tggtggtatg tttgaccttt ccaagactgg ggttaaagaa cccgtcttga
    10261 tttcagggac tgacggtgtc ggaaccaagc tcatgttggc tatcaagtac gacaagcacg
    10321 ataccatcgg gcaggactgt gtggccatgt gtgtcaacga catcattgct gcaggtgcgg
    10381 aacccctcta ttttctcgac tacgtagcga cagggaagaa tgaaccagct aagctagaac
    10441 aagtggttgc tggtgtggca gaaggttgtg tgcaggctgg tgctgccctc atcggtgggg
    10501 aaacggctga aatgccgggc atgtacggcg aagacgacta tgacttggct ggttttgcgg
    10561 tcggtgtggc tgaaaaatct caaatcattg acggttcaaa ggtggtagag ggagatgttc
    10621 ttctcggact tgcttcaagt gggattcact caaatggtta ctctttggtt cgtcgtgtct
    10681 ttgcggatta cacaggtgag gaagtcctac cagaattgga aggcaagaaa cttaaggaag
    10741 ttctacttga gccgactcgt atctatgtca aggctgtctt gccgctcatc aaagaagagt
    10801 tggtcaacgg cattgcccac atcacaggtg gtggctttat cgaaaatgtc cctcgtatgt
    10861 ttgcagatga cctagctgct gaaattgatg aaagtaaagt tccagtgctt ccaattttca
    10921 aaacccttga aaaatacggt cagattaaac acgaagaaat gtttgaaatc ttcaatatgg
    10981 gtgtgggact catgttggcg gtcagccctg aaaatgtaga gcgtgtaaaa gaattgttgg
    11041 atgaagcagt ctatgaaatt ggtcgcatcg tcaagaaaga aaacgaaagt gtcattatca
    11101 aatgaaaaaa atagcggttt ttgcctctgg taatggctca aattttcagg tgattgccga
    11161 agaatttcca gtggagtttg tcttttcaga ccatcgtgat gcctatgtgc ttgagcgtgc
    11221 aaagcagctc ggcgttctgt cctatgcttt tgaactcaag gagtttgaga gcaaggcaga
    11281 ctacgaagca gcccttgtcg aactcttgga agaacaccag attgacttgg tttgcctagc
    11341 aggctacatg aaaatcgttg gaccaacctt attgtcggct tatgaaggtc ggattgtcaa
    11401 cattcatcca gcctacttgc cagaatttcc aggagctcat gggattgagg atgcttggaa
    11461 tgctggcgtg ggtcagtctg gtgtgaccat tcactgggtg gattcgggtg tggatacagg
    11521 ccaggtcatc aaacaggttc gtgtgccacg actagctgat gataccattg acagatttga
    11581 agctcgcatc catgaagcag agtacaggtt gtatccggaa gtagtgaagg ctctatttac
    11641 agattgactt tttgatgatt catatgatat ctttgatttt aaattggagt cagtgtttgt
    11701 tgaagacggc ttcaaacgga ggtatttgta atgttagaat ctaaaaaaac aactcgatat
    11761 gtattttatg tctatctgat gttattaact tggggaatct tatttaagtt tgaaacaaat
    11821 cctgaattta tagcattttt cttagctcca aggtatatca attggattcc attttcagaa
    11881 ccactaatag tcgatggaaa aattgttttt gctgaaatgt tatttaatct gattttcttt
    11941 attccattag gtgtttgttt ccctttgata aaaactaatt tatctagttt aagaatagtc
    12001 gggacaggtt tcttgattag tttattgttt gagtacttac agtatatttt agcaataggt
    12061 ataacagata taacggattt gactttaaat acgctaggtg tctgtgtagg cttactgatt
    12121 tatcaaattt ttataagagt gttcaaatca cagactagaa aatggatcaa tatcttaggt
    12181 atgcttagcc ttggttttgc ttatcttgtt ttactgttac tgcatttaat tggtgtttaa
    12241 ctaatgatta aaaaggagaa tataatgact aaacgcgtct taatcagcgt ctcagacaaa
    12301 gcgggcattg ttgaatttgc ccaagaactc aaaaaacttg gttgggagat tatctcaaca
    12361 ggtggaacta aggttgccct tgataatgct ggggtggata ccattgctat cgatgatgta
    12421 actggtttcc cagaaatgat ggacggtcgt gtgaagaccc tccacccaaa tatccacgga
    12481 gggcttctcg ctcgtcgtga cttggatagc cacttggaag cggctaagga caacaagatt
    12541 gagctcattg accttgtggt ggtcaacctt tacccattta aggaaactat ccttaaacca
    12601 gatgtgactt atgctgatgc agttgaaaat atcgatattg gtgggccgtc tatgcttcgt
    12661 tcagcagcga aaaatcatgc cagtgttaca gttgtggtag atcctgctga ctacgctgtg
    12721 gttttggacg aattggcagc aaacggcgaa acctcttatg aaactcgcca acgtttagca
    12781 gccaaagtat ttcgtcacac agcggcttat gacgccttga ttgcagaata cttcacagct
    12841 caagtgggtg aaagcaagcc tgaaaaactc actttgactt atgacctcaa gcaaccaatg
    12901 cgttacggtg agaatcctca acaagacgcg gacttttacc agaaagcttt gcctacagat
    12961 tactctattg cttcagccaa acagctcaac gggaaagaat tgtcatttaa taatatccgt
    13021 gatgcagatg ctgctatccg tatcatccgt gacttcaaag atagtccaac cgttgtggct
    13081 ctcaaacaca tgaatccatg tggaattggt caagctgatg acatcgagac tgcttgggac
    13141 tacgcttatg agtctgaccc agtatctatc tttggtggga ttgtcgtcct caaccgtgag
    13201 gtggatgctg cgacagctga gaagatgcac ggcgttttcc tcgaaatcat cattgcacca
    13261 agctatacgg atgaagcgct agccattttg atcaataaaa agaaaaactt gcgtatcctt
    13321 gccttgccat ttaatgctca agaggctagc gaagtggaag cagaatacac aggtgtagtc
    13381 ggtggacttc tcgtgcaaaa tcaagacgtg gtcaaggaaa gcccagctga ctggcaagtg
    13441 gtgaccaaac gtcagccaac tgagacagaa gcgactgctc ttgagttcgc ttggaaggct
    13501 atcaagtacg tcaaatcaaa tggtattatc gtgaccaacg accacatgac acttggtgtt
    13561 ggtccaggtc aaaccaaccg tgtggcttct gttcgccttg ccattgacca agccaaagat
    13621 cgtctgaacg gggcggtcct tggttcagat gccttcttcc catttgcgga taacgtggaa
    13681 gaaatcgcca aagcaggaat taaggccatc atccagcccg gtggctctgt ccgtgaccaa
    13741 gaatccatcg aagcagcgga taaatacggc ttgactatgg tctttacagg tgtgagacat
    13801 tttagacatt aagaagataa aagggaagaa aacagtttct ttcctttttt ggcttaaaat
    13861 gctaactgaa acaagattaa aacgaacttt tttgatataa tgttggtaaa taattcgcaa
    13921 aagaggttga ggaatgaaac tgcttgttgt cggttctggt ggtcgtgagc atgcgattgc
    13981 taaaaagtta cttgaatcaa aagacgtgga aaaagtcttt gtagctcctg ggaatgacgg
    14041 tatgactctg gatggtctgg aattggtaaa tatatctatt tccgaacatt ataaattgat
    14101 tgacttcgca aaggccaacg atattgcttg gacctttatc ggtccagatg acgcccttgc
    14161 ggctggtatc gtggatgatt tccatgcagc tggtctcaaa gcctttggtc cgacaagatt
    14221 ggcagcggag ctggagtggt ccaaggattt cgccaaggaa atcatggtca aatatggtgt
    14281 ttcgacagca gcctatggca cattttcaga tttcgaggaa gcaaaggcct atatcgaaaa
    14341 gcatggtgcg cctatcgtag tcaaggcgga tggcttggcg cttgggaagg gtgtcgtcgt
    14401 tgctgagacg gttgagcaag cggtcgaagc cgctcatgag atgcttttgg acaataaatt
    14461 tggtgactca ggtgcgcgcg tggttattga ggaattcctt gaaggagagg aattttcact
    14521 ctttgccttt gtcaatggtg ataagttcta catcatgcca acggctcagg accacaaacg
    14581 tgcctacgat ggcgacaaag ggcctaacac gggtggtatg ggtgcctatg cgccagttcc
    14641 acacttacca cagagtgtag ttgatacagc ggctgacacc attgtcaagc cagttctaga
    14701 agggatgatt aaagaaggtc gcccttatct gggagttctt tacgcagggc ttatcctgac
    14761 agctgatgga ccgaaagtca ttgagttcaa cgctcggttc ggagatccag aaactcagat
    14821 tatcttgcct cgcttgacct ctgactttgc tcaaaatatc acagatatcc tggatagcaa
    14881 ggagccaaat atcatgtgga cggacaaggg tgtgactctg ggtgtggttg tcgcatccaa
    14941 gggctacccg ctagactatg aaaggggcgt tgagttgcca gccaagacag aaggcgatgt
    15001 catcacctac tatgcagggg ctaagtttgc ggaaaatagc agagcactgc tctcaaacgg
    15061 cggacgagtt tatatgctcg ttaccacagc agataccgtc aaagaagccc aagccagcat
    15121 ctaccaagaa ctataccaac aaaaaataga aggactcttc taccgaacag atatcggaag
    15181 caaggcaatt aagtaaagat ataagaataa cgcgccgtag tcgccaaaca cgataatggt
    15241 cgtcgtggtg aaaagaccag aacagtgaat gttctggtca ggggaaactt ggagacctta
    15301 ggctcaaagt ttaggaatga aaccgaaggt ttgcttccgc ctccatcacc taagaccatt
    15361 atcaaaaaga aaaataaaaa ttcacaaaat acgttaatga tcgtatggtt tgcgagcgtt
    15421 agcgagctaa tatagaacaa tcaccgccgt tgtgaaagaa cgattggatg ataatccaat
    15481 cgttcaggga aattggaaga ccttgggttt ccaatttagg catgagacac ctttggtggc
    15541 tgctgccgtc cctcacaagc taaggtgatt gttgaaaaag aggaaaaagg agaagaaatg
    15601 aaaccagtaa tttccatcat catgggctca aaatccgact gggcaaccat gcaaaaaaca
    15661 gcagaagttc tagaccgctt cggtgtagcc tacgaaaaga aagttgtttc cgcacaccgt
    15721 acaccagacc tcatgttcaa acatgcagaa gaagcatata gccgtggtat taaggtcatc
    15781 atcgcaggtg ctggaggcgc agcccacttg ccaggaatgg tagctgccaa aacaaccctt
    15841 cctgtcatag gggtacctgt caagtctcgt gctcttagtg gagtggattc actctattct
    15901 atcgttcaga tgccgggtgg ggtgcctgtt gcgaccatgg ctatcggtga agctggagcg
    15961 actaacgcag ctctctttgc cctccgtctc ctctctgtag aagataagtc cattgcggat
    16021 gcacttgcca actttgctga agaacaagga aaaatcgcag aggagtcgtc aaatgagctc
    16081 atctaaaaca atcggaatta tcggtggcgg tcaactgggt cagatgatgg ccatttctgc
    16141 tatctacatg ggccacaagg ttatcgcgct ggatcctgcg gcggattgcc cggtctctcg
    16201 tgtggcggaa atcattgtgg caccttataa cgatgtagac gccctccgtc agttggcaga
    16261 ccgttgcgat gtcctcactt acgagtttga aaatgtcgac gctgacggtt tggatgccgt
    16321 tatcaaggat ggacaactcc ctcaagggac agacctgctc cgcatttcgc aaaatcgtat
    16381 ttttgaaaag gactttttgt caaacaaggc tcaagtcact gtggcaccct acaaggtcgt
    16441 gacttctagc ctagacttgg cagatatcga cttgtcgaaa aactatgtcc tcaagactgc
    16501 gactggtggc tacgatggtc atggacaaaa ggttattcgt tcagaagtag acttggaagc
    16561 agcctatgcg ctagcagaat cagcagactg cgtcttggaa gaatttgtca actttgccct
    16621 tgagatttct gtcatcgtgt caggaaatgg caaggaggtg acgtttttcc cagttcagga
    16681 aaatatccac cgcaacaata tcctgtctaa gaccatcgta ccagcccgca tttctgaaag
    16741 tctagtagac aaggctaaag ctatggcagt gcgaatcgca gaacaactca acttgtctgg
    16801 aactctctgt gtggaaatgt ttgcgactga tgatgacgtt attgtcaatg aaatcgcccc
    16861 acgaccacat aactctgggc actattctat tgaagcctgt gatttctctc agtttgacac
    16921 ccatattctc ggtgttctcg gagcaccatt accagtcatc aaactccatg cgccagccgt
    16981 tatgctcaat gtcctaggtc aacacgtcga ggctgctgaa aagtatgtcg cagaaaatcc
    17041 aagcgcccac ctccacatgt atggtaaaat agaagcaaag cataatcgta agatgggaca
    17101 tgtgaccttg tttagtgatg taccggatag tgtggatgag cttggggaag ggattgattt
    17161 ttaggacaag tctatgatac aaattatcgt taatacattt attgaaaagg ataagactgg
    17221 agcagttgtt gaagtgttgt atgccagtgc tgaccaagat aaggtacaag ctaaatatga
    17281 agaactagct gcacaatacc ccgaaaatta tttagctatc tatgatgtac cgctggatac
    17341 ggatttgaat acactagatc attacccgtc tgtgtttatt gaaaaagagg agtttgagta
    17401 gaaatcttgg tttacctaga tagcttattc ccaacagctt aagaagaaag gaaaaattaa
    17461 cacatgatca accgttactc tcgccctgag atggcgaata tttggagtga agaaaataaa
    17521 taccgtgctt ggcttgaggt ggaaatcctc tctgacgagg catgggctga gttgggggaa
    17581 atccctaagg aagatgtggc tttgattcgc aagaaggcgg actttgacat cgaccgtatt
    17641 ttggaaattg agcaggagac gcgccacgat gtggtggctt tcacgcgtgc ggtttctgag
    17701 actcttggtg aagagcgcaa gtgggttcac tatgggttaa cttctaccga cgtggtggat
    17761 actgcttatg gttacctcta caagcaggcc aacgacatca tccgtcgtga ccttgaaaac
    17821 ttcactaata tcatcgctga caaggccaag gagcacaagt tcaccatcat gatggggcgt
    17881 actcatggtg tgcacgctga gccgacaacc tttggtctta aattggcaac ttggtacagc
    17941 gaaatgaaac gcaatatcga gcgtttcgag catgcagctg ctggtgtaga agctggtaag
    18001 atttctggtg cggttgggaa ctttgccaat atcccaccat ttgtagagga gtatgtctgc
    18061 gataaacttg gcatccgtgc ccaagaaatc tctacacaag tccttcctcg tgaccttcac
    18121 gctgagtact ttgcggttct tgccagcatt gcgacttcaa tcgaacgtat ggcgactgag
    18181 attcgtggtc tacaaaaatc tgagcaacgc gaagtagaag agttctttgc taaagggcaa
    18241 aaagggtctt cagcaatgcc tcacaaacgc aacccaatcg gttctgaaaa tatgactggt
    18301 ctggcgcgtg tcattcgtgg tcacatgatt acggcttatg aaaacgtcgc tctctggcac
    18361 gaacgcgata tttctcactc atcagctgag cgtatcatca caccagatac gaccattttg
    18421 attgactaca tgctcaaccg ttttggaaat atcgtcaaga acttgacagt cttcccagaa
    18481 aatatgatcc gaaacatgaa ctcgactttt ggtcttatct ttagccaacg ggctatgttg
    18541 acattgattg aaaaaggcat gacccgtgag caagcctatg acttggtgca accaaaaaca
    18601 gcctactctt gggacaacca agtagacttt aaaccacttc ttgaggcaga ttcagaagta
    18661 acatcacgtc tcacacaaga agaaatcgat gaaatcttca acccagttta ttacaccaaa
    18721 cgagtggatg atatctttga acgtcttgga ctaggtgatt aattaaaaaa taaacagcga
    18781 gcttcaatct cgctgtttat tttttatcga aaagacttag tcttcttttc ttttagtgag
    18841 tccataggct gctagtgtgg ccatgagtcc tgcgactact agtcctgcag aatcgtgagt
    18901 tcctgtttca ggaagttttt tctctattac cacaggagct ggatcttgag gaagagcttt
    18961 gctttcctca gcaggagctg gttggcttag gatttctagt tttggttttt cttcagcaat
    19021 agcggcttgt ccgttttcat cgcctacatg tgttaccata gttccgactt cgactatttg
    19081 agtaacggct tcctgtgcta cgacactatt tacaagtgtt ttcacttcct taccatcggc
    19141 agaagtgctc acagagtaga agttgctacg atgtccattg acgcccttag taatgacttg
    19201 tgtttttcct ttgagtaaga gtggattttc acgagtcact gtggtaaatg gaatttcttc
    19261 ttcttggata tccagtctag gttttacctc agtagttggt gcaagaccac tttcatcacc
    19321 cttgtgagtt acaggagcgc caacttcaac cacttggttt ataacttctt tggttacctg
    19381 gctatcaagg actgtttctg ttgtttttcc attttcagtg agtacagaga tgtaatgagt
    19441 tcgttcacct ttgactcctg ctgtgataat attttcctga ccagctggga ggttaggatt
    19501 ttctttcttg ataacttcaa atggaatttc ttcagttctt gtgatgagtt ctggtctggt
    19561 ttcaacattg gcagccactt cattttcatc taggcttcct gaatgagttg cagctggttt
    19621 gaggccttga agagcggctt ttaggttggc tacaagtgtg tcaagctcag cttgtttatt
    19681 acggttgagg ttgtaattta gagctgtttt agctgcgtca agggcctcaa gactttcttt
    19741 actatatcct tctaagtttg taggaatttt agctaattct tcgcggagag cattataatt
    19801 agcacggaag tagtctttgt tgtggtctgc aaaggcagtc atgagttcaa agatttcctc
    19861 ttccttgtat tcagcgcttg gtctatctgc ccagattgaa agcatacttc cgactgttgg
    19921 aagatctact tcaggatatt tggtagaagc tagttgattg aatggaactt taccagaatt
    19981 ttcaactgct tttgaaagag ggtaagcttc atcttgtttg tgattgccta taacataata
    20041 ccagtcaccg ttggtattca agaacttata acccttgctt gctaggtatt gaggtgatgc
    20101 gaggttgtat ccccaccagc ctttagacca gtaagaaatc aagacatctt tgtcaaactg
    20161 aacatcgtcc ttgtcttcat agtagaagcc atcgttgaag gccattggtt gaagccctct
    20221 ttctttggcc atagctgcga gggtgttggc atattcggca aatttgccat agagttgata
    20281 ccacttgagg tagtaccagc cttgggcact agtcgcatcg ttggcgtatt cgtcagtacc
    20341 aaagttgaaa atctttgttt tacctgcaaa gaagtccatg tatttaccga tgagggcttt
    20401 tacaaagttc atcgcttctt cgtttttcaa gtccatagtt gtttttgaaa ctttatcaaa
    20461 gtgggcttga ggatttttaa tacctaattt ttccatggca accagcatag catccatgtg
    20521 acctggactg ttaatagctg ggatgagacc aatgtcctta gatttagcgt attcaattag
    20581 ctctgtcact tctgcctgtg ttagtgcagt accgtttgga tcgtcgtagt aagctttagt
    20641 tccttcgata atagcttttt taacgtcatc actagcatag gtttttccgt tggcagtaat
    20701 ggtcatatca ttgagtagaa agcgaagtcc gtcatttcct agaaggagat ggacatcaga
    20761 atatccgagc tcactggcct tgtctacgat gcgtttgagc tggtccagag taaagtattt
    20821 gcgtccagca tcgattgaga ttaccttgtt tttggcaagt ttttcaacct cacgtttagc
    20881 ttcttcttct ttttgagctt caggcgtgag ggtcaagttg ttgacagttt cttgaagttt
    20941 agcaatggct ttatcaatcg tatcttgttg ggcacggcta aggttgctat cgagagagcg
    21001 aatagctttt tcagcttctt ttacggccgt gacgctttct gcagtataac ggttcaggtc
    21061 ttttggtacc tcgttaagtg cttgctctgc agattcataa tcagctgcga agtattcagc
    21121 gttggcattt gcaaaatgac gcatgagttt gaagaggcgt gatggtgaat aacgtgcaga
    21181 tggagtgtca gcccaagcag ctaccatacc accgatgatt gggatatcag ctccttctgt
    21241 ttttggtaca gaagtgattg gtgtgttttt aataccattg agcccctgat cgagattgta
    21301 ccagccttgg ccatcagcgt ttcgtccaag aacgtagtac caagcatcat tggtattaag
    21361 gatttggtga cctttttcag ctagtagttt agaagaagcg acatcgtagc ctccccaacc
    21421 accagtccac atagaaacga tgatgtcttt gtcaaaacta ccaaagcttg tgtcgctatt
    21481 gtagtagata ccgtcgttaa aggccattgg tttgagaccg tgcgatttta caatacgagc
    21541 gaggtcattg gcgtaggcaa taaatttttc atagcctttt acagggtagc cttcgtttgg
    21601 atagtattta tcagcttgaa gcacactcca acctttagca tctgtcgcat cattggcata
    21661 ttcatcaagt ccgatgttga agatttcagt ctttttcgcg aaataagcag catacttgtc
    21721 gataagggct tttgtaaaag cgacagcttg ttcgttgtca agatcgacag tacgggctga
    21781 tttcttccca aaatagctaa agttagggtt ttggattccc aattctttca tggcattgag
    21841 aatcgcatcc atgtgtccag gactatttac tgtcggaatg agaccgatac ctttatcttt
    21901 ggcatagtta atcagatctg tcatttgact ttctgttaag tgattgccgt ttggatcgtt
    21961 gtaataatca tttgtacctt tttcaatggc gcgtttgaca tcgtcactgg cataggtctt
    22021 gccgttagct gtgatgctca tatcgtccaa catgaaacgg agtccatcat ttccgactaa
    22081 taggtgtaaa tcagtgtagc cataatgttt cgctttatcg atgatttcct tgagctgttc
    22141 tggtgagaaa tatttacgtc cagcatcaat agaaacaatt ttctttttcg ctagtttttc
    22201 atttacagtt gcagcacgtt cctttcctgt ctctgtttcc ggtttgtcag cctctgcttt
    22261 cgcttcatct tttttagctg gtttatcctt gtcagtcttg tctgtatttg actctttaga
    22321 atcaacctct ttcgcttctt cctttttagg gctagcttct tcttcctttt tattagcagt
    22381 ttctttttca gcagaagttg gagttaccac ctctgcttta tcactaggag ttgaactaac
    22441 ttcctcttgt ggtttttctt ctgtttttgg aagactagct accttatcag tagctggagt
    22501 ttctgtttct acagtttttg gagcctctgg ttgaagctct gctttaggtg tttcctcagt
    22561 ccgattttcg gatgattgag gggaatcaga aaccgtatgg atggtcggtt ggttttctgt
    22621 agtagtagga gtaactccat cggctgcaac agtctgtgct tggaaggcaa atccaattag
    22681 aacagaagct gctcctacag cgtatttacg aatagaaaaa cgctgttgtt tttcatgttt
    22741 cattgcaaaa cctcctgatt gcattgttat attgatagcg attatataaa tcaacgccct
    22801 tattttattt cttatattaa cgagagtcaa gaggagatga caaaaactat aataagtata
    22861 aaaaaatata aaatttaaac ttaagatttc agattggtcg gaaaaaatgc atatatatct
    22921 agtataattt ttggttctat ttctataaaa tattccacaa attatagaat cttccaaaaa
    22981 taggtaagcg ctaccttttt ggtgtggtat aataagcata gaaaaagccc gagcgattag
    23041 gctcaggctt tcttcttagt gatcacggtc acatgagatg aatttaatct tgtagtaatc
    23101 agatcgtttg taagtttcac tgtattctaa aacttggcca gttgattcga gtttggtgat
    23161 tttagtttgt aggacagtag ggaattgttc atcgactccg aggactgaag ccgcatgttc
    23221 tggagttgga aagactattt cgttgatttc ttcaaagtgt tcatcattca tgtgaatgtg
    23281 gtagtctaac ttgaaacgat tatagataga actatagtat tcaaggtttg gataatttgc
    23341 gttgatatat tgttctggga tgtaggatgt atggtagata taaacgacac cgtttgattc
    23401 gcggatacgt tcaatcttgt agtagaattg atcgccgcgt agacccaatt tttccaagta
    23461 aacaagcttg tttccgcgtt caattgaaag aacagttacc ttatcatctt tagcattgaa
    23521 gagttcaata tctgaaaact ctacaagctt gtgtttgcgt gcacgtgaaa cgaaggttcc
    23581 ttttccttgt tggcggacaa tatagccatc tttggcaagg tcgtttaagg cgcgaacaac
    23641 tgtgatagag ctgacatcgt acattgaaat gagttctgct tcagtgtaaa atttatctcc
    23701 actgctaaac tgcccagaga tgattttatt ttttaattcg tcttttatgt attgatattt
    23761 gggaattgcc atatttttac ctctattttc cttctccata gaaaatttta ccataaaagc
    23821 gaaaaaaata gtagtctttt g
//
LOCUS       DAVMGZ010000016        23415 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000016 DAVMGZ010000000
VERSION     DAVMGZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.017"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..154)
                     /locus_tag="VYQ93_000532"
     CDS             complement(<1..154)
                     /locus_tag="VYQ93_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV3335272.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKME"
     gene            complement(167..547)
                     /locus_tag="VYQ93_000533"
     CDS             complement(167..547)
                     /locus_tag="VYQ93_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335273.1"
                     /translation="MIDLYLSKNSQRNQLLLDFFQKYSIEVTPHSISEMTRDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFIEMILKNPELSIRLPLAVSNKRVYPNLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            complement(540..989)
                     /locus_tag="VYQ93_000534"
     CDS             complement(540..989)
                     /locus_tag="VYQ93_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335274.1"
                     /translation="MKALNKESILDCDELETELHDAEIKQLDEQLFLIPNYPCEFEVT
                     FLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDNQNLVFVLYGQGYRAEG
                     KEGILTTQVTVKAYDEDKKPINFANLLDSLIVSEYQMEPNLWEVSHD"
     gene            complement(973..2331)
                     /gene="dcm"
                     /locus_tag="VYQ93_000535"
     CDS             complement(973..2331)
                     /gene="dcm"
                     /locus_tag="VYQ93_000535"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEV3335275.1"
                     /translation="MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQT
                     EGEIEFHDIRDVSDDEFKKLRGKVDIICGGFPCQAFSIAGRRLGFEDTRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRE
                     RVFIIGHSRKKSTRLLFPFRREGQATNSEILKTLGNLNPSKSGMSGKVYYSEGLAPTL
                     VRGKGEGFKIAIPCITPDRLDKRQNGRRFKENQEAMFTLNTQDRHGIVVVGDLPTSFK
                     ETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIKFLKVREATKKGYAQAEIGDSINLE
                     RPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGVLIDGQFYRLRIRR
                     ITPKECFRLQGFPDWAFEAARKVSSNSQLYKQAGNSVTVPVIAAIAKKLKEIEEKDES
                     IK"
     gene            complement(2430..3209)
                     /locus_tag="VYQ93_000536"
     CDS             complement(2430..3209)
                     /locus_tag="VYQ93_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV3335276.1"
                     /translation="MKRITANQYQTSERYYKLPKILFEDEKYMDMKLEVKVAYSILKD
                     RLELSLNRGWIDEEGAVFLVFSNSKLMRLLGCSKSKLLSIKKTLKEYDLIDEVQQSSS
                     VKGRLANKIYLGELSSTPVDNSNRPSVNKRLGQVENETAPVSYSAPSETEVSETKYSE
                     TDSLFTEDEEDRNTHPILKRKVEKVTKYDRDYIWGLVQDQFRREGFSETASEIAMTDF
                     ERIYQYALDNVRFVRRAEALAEFVFNGLYSVWNNRVRKGGG"
     gene            complement(3206..3379)
                     /locus_tag="VYQ93_000537"
     CDS             complement(3206..3379)
                     /locus_tag="VYQ93_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335277.1"
                     /translation="MTKELQSSRYIVISFLVREMGIDIVEAISLMAELEKSGLVRLES
                     SGDLILKELGGAL"
     gene            complement(3588..4949)
                     /gene="rlmD"
                     /locus_tag="VYQ93_000538"
     CDS             complement(3588..4949)
                     /gene="rlmD"
                     /locus_tag="VYQ93_000538"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV3335278.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLFRRFDLKPYDEKEHSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVECVVLLQR
                     KKG"
     gene            complement(5023..5997)
                     /locus_tag="VYQ93_000539"
     CDS             complement(5023..5997)
                     /locus_tag="VYQ93_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV3335279.1"
                     /translation="MRKKLFLTGAAVLWAVTAMNSAHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(6071..6685)
                     /gene="pmp23"
                     /locus_tag="VYQ93_000540"
     CDS             complement(6071..6685)
                     /gene="pmp23"
                     /locus_tag="VYQ93_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV3335280.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(6685..7659)
                     /locus_tag="VYQ93_000541"
     CDS             complement(6685..7659)
                     /locus_tag="VYQ93_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV3335281.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(7667..8923)
                     /locus_tag="VYQ93_000542"
     CDS             complement(7667..8923)
                     /locus_tag="VYQ93_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV3335282.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLAAGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            complement(8985..9416)
                     /locus_tag="VYQ93_000543"
     CDS             complement(8985..9416)
                     /locus_tag="VYQ93_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3335283.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(9418..10020)
                     /locus_tag="VYQ93_000544"
     CDS             complement(9418..10020)
                     /locus_tag="VYQ93_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV3335284.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(10004..10843)
                     /gene="prmC"
                     /locus_tag="VYQ93_000545"
     CDS             complement(10004..10843)
                     /gene="prmC"
                     /locus_tag="VYQ93_000545"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV3335285.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(10843..11922)
                     /gene="prfA"
                     /locus_tag="VYQ93_000546"
     CDS             complement(10843..11922)
                     /gene="prfA"
                     /locus_tag="VYQ93_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV3335286.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(11919..12509)
                     /locus_tag="VYQ93_000547"
     CDS             complement(11919..12509)
                     /locus_tag="VYQ93_000547"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV3335287.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            12632..12814
                     /locus_tag="VYQ93_000548"
     CDS             12632..12814
                     /locus_tag="VYQ93_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV3335288.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            12950..14323
                     /gene="mnmE"
                     /locus_tag="VYQ93_000549"
     CDS             12950..14323
                     /gene="mnmE"
                     /locus_tag="VYQ93_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV3335289.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIVQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEQLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(14370..15305)
                     /gene="dapA"
                     /locus_tag="VYQ93_000550"
     CDS             complement(14370..15305)
                     /gene="dapA"
                     /locus_tag="VYQ93_000550"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV3335290.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(15361..16437)
                     /locus_tag="VYQ93_000551"
     CDS             complement(15361..16437)
                     /locus_tag="VYQ93_000551"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV3335291.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(17023..17095)
                     /locus_tag="VYQ93_000552"
     tRNA            complement(17023..17095)
                     /locus_tag="VYQ93_000552"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17060..17062),aa:Thr,seq:ggt)
     gene            complement(17195..17704)
                     /locus_tag="VYQ93_000553"
     CDS             complement(17195..17704)
                     /locus_tag="VYQ93_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV3335292.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(17702..17798)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            17827..18258
                     /locus_tag="VYQ93_000554"
     CDS             17827..18258
                     /locus_tag="VYQ93_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV3335293.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            18340..18717
                     /gene="mscL"
                     /locus_tag="VYQ93_000555"
     CDS             18340..18717
                     /gene="mscL"
                     /locus_tag="VYQ93_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV3335294.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(18759..19853)
                     /gene="hemH"
                     /locus_tag="VYQ93_000556"
     CDS             complement(18759..19853)
                     /gene="hemH"
                     /locus_tag="VYQ93_000556"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV3335295.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            20008..21231
                     /gene="pepT"
                     /locus_tag="VYQ93_000557"
     CDS             20008..21231
                     /gene="pepT"
                     /locus_tag="VYQ93_000557"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV3335296.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKEQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASIQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(21267..21896)
                     /locus_tag="VYQ93_000558"
     CDS             complement(21267..21896)
                     /locus_tag="VYQ93_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335297.1"
                     /translation="MSKVNQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIEIASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            21861..22082
                     /locus_tag="VYQ93_000559"
     CDS             21861..22082
                     /locus_tag="VYQ93_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335298.1"
                     /translation="MDLFQELIYLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(22191..22667)
                     /locus_tag="VYQ93_000560"
     CDS             complement(22191..22667)
                     /locus_tag="VYQ93_000560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV3335299.1"
                     /translation="MTPNNGVSAADDGYVFNPNDIVRDTGDAYIVRHGDHYHYIPKSS
                     LNNPPSHSNTEEAGSSSSSVLSNPSLHVHHEEEDGHGFDANRIISEDSEGFVIPHGDH
                     NHYIKVQTKGYEAALKNKIPSLQSNYQPGTFDEKAVLAKVDQLLADSRSIYKDRLS"
     gene            complement(22707..22847)
                     /locus_tag="VYQ93_000561"
     CDS             complement(22707..22847)
                     /locus_tag="VYQ93_000561"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV3335300.1"
                     /translation="MLEITLKSPYQFAHILFQPTIVPHGGHYHFIPESDLSAGELAAA
                     KV"
     gene            complement(22891..23415)
                     /locus_tag="VYQ93_000562"
                     /pseudo
     CDS             complement(22891..23415)
                     /locus_tag="VYQ93_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         6780 a         4955 c         4314 g         7366 t
ORIGIN      
        1 gttccatctt gtagtggcgt atcttttgat acaaacgatt caattcactt ggatagtgaa
       61 actctcccgc aaacattttt ctggttaact caatccagct gatatttctt tcagccaaaa
      121 taatggacaa gttctcccaa aatcgttcag ccatattgct tctcctttag ttagataaat
      181 aatgtgtttg tgccatgtaa atcaattgtt tcgtatctct tggcaataga gctctagcct
      241 cttccaaatt cagatttgga taaactctct tatttgaaac tgcaagagga agtctgatgc
      301 ttagttcagg attttttaat atcatttcga tgaaatccgt taatcttaga ttgtcacgat
      361 tcttaaatcg taataaattg ggagataaaa actcaaaaca atctgaagaa tagctcatca
      421 tttcaattaa tttatccctt gtcatttcag aaatagaatg aggagtgacc tctatgctat
      481 acttttgaaa gaaatccaaa agaagttgat ttctttggct atttttactt agatagagat
      541 caatcatgag agacctccca aagattcggt tccatttgat attctgagac aatcaaggaa
      601 tctaataaat ttgcgaagtt aatcggtttc ttgtcttcat cataagcttt tacagttact
      661 tgggttgtaa gtattccctc ttttccctcg gctcgatagc cttgtccata taaaacaaaa
      721 acaagatttt gattatcatc tacaaaggca tcaaccccat tctttatgtc ttgactttca
      781 aggaattcca taacgttttg aagataggac tcgtaaaata gtgggtagtt gtgtttttta
      841 tggtaatcat ctaaaaatgt tacttcaaac tcacatggat aattgggtat caaaaataat
      901 tgttcatcca gctgtttgat ttctgcatca tgtaattctg tttctaattc atcacaatct
      961 agtattgatt ctttatttaa tgctttcatc tttttcctct atttctttta atttctttgc
     1021 gattgcggca atcacaggaa cggttacact attaccagct tgtttataga gctgactatt
     1081 actagagact tttctagcag cttcaaaagc ccaatcagga aagccctgca agcgaaaaca
     1141 ctctttagga gtgattcgtc gtatcctcag acggtaaaac tgtccatcta ttaatacacc
     1201 ggctacatga taaacttgtt tatcttctcc ttcataacta gcaactacta ctcccatttg
     1261 tccactagtt gttaacgtat tagctatacc ttttccaact ctaccacgac gatactgaga
     1321 acttggtctt tccaaattaa ttgaatctcc tatttctgct tgagcgtatc cttttttcgt
     1381 tgcttcccgt acttttagaa atttgattgg ttctggaatt agtattttgg gaattttatc
     1441 tccaccttgc attgtagtca gtgttggaga taacccctca cttccataaa ctcgaccagt
     1501 ttccttaaag ctagttggta aatctccaac aacgacaata ccatgacgat cctgagtatt
     1561 taaagtaaac atcgcctctt ggttttcctt aaagcgtcta ccattttgtc tcttgtctaa
     1621 tctatctggt gtaatgcaag gaatcgcaat cttaaatcct tctccttttc cacgaactaa
     1681 ggttggcgca agaccttctg aataatagac tttaccgctc attccacttt ttgatggatt
     1741 caaattccct aatgttttta aaatctcaga gttagttgct tgaccttctc gtctgaaagg
     1801 aaataagagt ctggtacttt tctttctaga atgtccgata ataaacaccc tctctctgtt
     1861 ttggggaacg ccgaaatcct tactgttaag cacctgccac tcaacatcaa accccaactc
     1921 atcaagtgtg gtaaggattg tggtgaacgt ccgtccctta tcgtgattga gtaggccttt
     1981 aacattttca agaaaaagaa aacgtggttg gatttgtttg gccgcccgag caatttcaaa
     2041 gaacaaagtt cctctagtat cttcaaatcc caatcgtctt cctgcgattg aaaatgcttg
     2101 acaagggaat cccccacaga tgatatcgac tttccctcta agttttttaa attcgtcatc
     2161 tgaaacatct cgtatgtcat gaaattcaat ttctccttcc gtttgaaaaa tggacttata
     2221 agattctcta gcaaatttat caatctcaca aaatccaata cactcgtgtc caacactttc
     2281 cattcctagt cgaaatccac ctatacctga aaataaatca ataaatctca tttcttgatc
     2341 acccctttct gatcgacaat caacgtccaa agagttatac cacttgaaat taaaatgagc
     2401 ataactaata tccactcgat tggcgacatt taaccacctc cttttctaac acggttattc
     2461 caaacagaat acaagccgtt aaacacaaat tcagcaagtg cttccgctcg tctaacaaag
     2521 cgaacattgt caagagcata ctgatagatt ctctcaaaat cagtcatagc aatttcactg
     2581 gctgtttcag aaaaaccttc tcgtctaaat tgatcttgta ccaatcccca aatataatct
     2641 cgatcatatt ttgtgacctt ttctactttt cttttcaaga taggatgagt attcctatcc
     2701 tcctcatcct cagtaaataa agaatcagtc tcactatatt tagtctcact aacttcagtc
     2761 tcactagggg ctgaatatga gacgggggcc gtttcatttt caacctgccc tagtctttta
     2821 ttaacactag gcctgtttga attatctact ggggtagaag ataattctcc taaataaatc
     2881 ttattagcta gtcttccttt aacacttgaa gactgttgaa cttcatcaat taagtcatat
     2941 tctttaagag tttttttgat ggacagtaat tttgacttcg aacaacctaa cagcctcatc
     3001 agtttagaat tagaaaatac taaaaaaacc gcgccttctt catctatcca accacgattg
     3061 agagataatt ctaaacgatc ttttaaaata gaataagcca cctttacttc tagtttcata
     3121 tccatatatt tctcatcctc aaaaagaatt ttaggtaatt tataataccg ttctgaagtt
     3181 tggtattgat ttgcggtaat tcgtttcata gcgctcctcc aagttctttg agtattaaat
     3241 ctccacttga ttccaatcga accaagccac ttttttctaa ttcagccata agagagatgg
     3301 cttcaacaat atctattccc atttcacgta ctaaaaatga aatgacaata tagcgtgatg
     3361 attgtaattc ttttgtcatt ttccctcctg aaaataaaaa aaggagaaca aaatacaatt
     3421 gttctctctt tagataaata tttattgttg tattcatttt tccaatgcgc aaacgacatc
     3481 tacaaataca tatacatcta tgtgcctttt taggactttt ttgacgtcaa aattgccttt
     3541 atctacaaac acaaaatgct tgtaaaatag cttaaaatca aggattttca cccttttttt
     3601 cgttgtagca acactacaca ctccacgtga tgcgtttgag gaaatagatc caccggctgg
     3661 actttcttca attcatatcc caactcttgg tatagtttaa tatcacgcgc catggttgcg
     3721 acattgcagg agatataggc gatgcgatcg gctcctgttt gggcgcttgc tttgataaag
     3781 ctttctgtca agcccttgcg tggaggatca accaagataa cggttggttg aatcccatct
     3841 ttaagccaat tcttcatagc attttcagct gtgtcacaga catagtgggc gtttgaaata
     3901 ttgttcagct gagcattttt tttactattc tcaaccgctt ctggaatcag ttcaacaccg
     3961 tagacttctt tgacatgctt ggcgactgat aaaccaatgg ttccaatacc agaataagca
     4021 tcaataacca cgtcatcttt ttttaactct gcaaagtcaa tggctgtttg atagagtttc
     4081 tccgccattt cagtattgac ttggtaaaag gctgggccag cgatttggaa gtcatttccc
     4141 aacatctggt ccgtaatata gtcttgacca taaagagtgc gccactcctt accaaaaatc
     4201 gcattggtat tctggtcgtt gatattttgc atgacagaca caatctctgg gaactgcttg
     4261 ataacttgtt caatcaattg gtcaacacga aaaacttttg gacgagttgt caccaaaacg
     4321 accatgattt gccctgaata gtgaccacga cgcaccacaa gattccgaat caatccagaa
     4381 tgttcctttt cgtcataagg ttttaaatca aaacgacgga acaggtctcg aagagctact
     4441 acgacttggt caatgacagg atcctggata aagaaatctt caaggggcat gaggttgtgc
     4501 gaattcttac ggaaaaatcc tgtttccaag acaccattca ctcgacgaac gggcacctgc
     4561 gccttattgc gatacttgac tggatgttcc ataccaagcg tttcagcaac ttctacatct
     4621 gcaattccag caatcttgta gagactgtcc ttgacttgct tggttttaaa cttgagctgt
     4681 tctggataag aaaggtgtcc taaatccgcg attcctgaac gcaggtaagc caaatctaga
     4741 tcttgattac ggtgtggtga ctggacaagg tatttttcaa cttttccaaa gccaatcttt
     4801 ttattgacct tgaggacacg catgagaatt ttttcactcg gtaaagcatt ctctacaaaa
     4861 aagaccaaac catctacctt ggcaactcct gccccttcat gggtcaaatc aacaatttca
     4921 acttctacaa tatcattttt ctttaacatt cttttctctt tctattggcc gacaaaaaag
     4981 acggcaacgt tacggttacc atccattata ccatgaaatt tcttaagaac caaaactctt
     5041 gaagaagttg acaagggctt gccagaggga gcttaagaag ttaccaccgt cctctagcgc
     5101 cttatccgca tcaaagttaa ggttgatatt tttaaaactg tcgccagctt gtgatacgat
     5161 gctttgttta aggtcattta gggttttagt gaaatctgca ttgctgagga tatcactctt
     5221 tgagagattc aaggcaaaat tgatgatgat attgatctgg tttcctgtta tgacctgatc
     5281 aagtttgtaa ttttttaagg tatcttcaac aatcttgcgt acatcttctt ctgtcagatt
     5341 tcccttgctt tctttagctt tggcgagtcc tgacttgata tcagctaggg caacgtttaa
     5401 tttattagca tcatagcctg atttgtcctt gttttcagca ttgatatctg acaaagcctt
     5461 tagctcttct tgagccaaat ctttattggc ttgcggcacc ttggctccat tagcttctag
     5521 cgaatagtag atcccagcca aagcactctc acctgtaact ggaatagggg ctgctacagt
     5581 gattttggca tgttccatac ccagcgttac tgctgcgttt cggtacatat cctgagtcac
     5641 cttagtgata ttttctggtg tttcaatctt gacctcaagt ggcgatttgt cacctagctt
     5701 ttgaatcttg gctgatgaat acaactgtaa gctagagtca ttggccacat tcatgatttt
     5761 agaataaaca tcaggtgtca tggtcttgag ttctttggta tctgttgagg cattgtagcc
     5821 cagtttttta agagtttgat ttttttggtc ttcagatagg gaggaaccta ggacatattc
     5881 aggttggaca taggtttcat cgataacttt ttgaacatct gttgctgcat gggcgctatt
     5941 catagctgtt actgcccaca agaccgcagc accggtcaga aagagtttct ttctcatagg
     6001 gaatttcctc ctttactttt ctagagtaat atatctatct taaagaaaac ttataacaaa
     6061 aacacctggt ctagccagat gttgaaaaga gagtgaaaca tttgatgatg taaaggttaa
     6121 gtcgtacctg tctagaataa taatagtttc ctccatttac atagagttca gcaccgtgaa
     6181 aaatggaaat ggggtgaata taactataag tctttccagt cctattacca agcaaggggg
     6241 caacagtctc acgagagtac tgtttggcta gagccaaggt attttccttg ccattttggg
     6301 cgataaaatc gatataggca ggtccaaaat tataggcttg aacagctgtc cagatatcta
     6361 cacccttctt ctgcgccaga tagagattgc ctgtcagagt ttgaatgcct tgccgaatgc
     6421 tagaggcatt atcattgatg gtgttggtgg aaccacttgc agactcacta gactgcataa
     6481 catcgccttc ttttcctttt gtttcagtat aaatcatagc aagcacaagc tcttcgtttg
     6541 ctggggtgtc tttttcactc aatatttctc gcaccatggg ttgataggtc atgacttgtt
     6601 tgacatcttg atgaacgcgg taagctttat agccagcaaa aaggaagact gctagtacaa
     6661 gcactcttcg aattcgttta aacattattt actttggata tcctcgatat ttttgattaa
     6721 gatagagtag gttccatttt cgttttggat aaactcaaca gactcggcgt cttgatagac
     6781 gttattggga acgatgagct caattccatt tgataaggag agtttttggt tttcaaattt
     6841 ctttaattgg cgactggcat caatttcatc aaattgaaca ggttctggta cggcttcttt
     6901 gacttggtca ataaagctca aacgagccgt cagattgttg tcaaaaaggt cattagccaa
     6961 tttctcaggt gacaattcat tgctttcttc taggttgttg aaaatagctg atttgacctt
     7021 ggattgaaat tgaaaatcat ctgtgttaaa agattcagca attctctggg ctgttttttc
     7081 cagttccttg atagattttt taggagaaat cttaggagcg acagcaagaa gattatctga
     7141 aaaatagttc aaaaaagtcc cgttgtactt gattcgtttt tcaatcaggt gatacttgcg
     7201 actctgaaga ttgaccacca aggcctcgtc agcacccgtt ccaaatccag gcaggttatt
     7261 ctgagtcagc ttgattggat tatcaacttc tcctccgagg tgggtcaagg tctcccgcag
     7321 ggcaattcgc aagaaagcga aatgttctac accttcttta gaaaattgaa caaaaatcaa
     7381 gtcattggtc ttgagatttt cagaaatgct aaactcctct ttccagagat tagccagcgt
     7441 tactgatgtc tccaacaaat cgtctgtaat atgattgaag aagggatttt cttcttcgaa
     7501 aatcccagtc ttggcttcat ctgaatacac atgttcaatt tttttacgta ggtattcttc
     7561 gatttttgga gtaatattga gaaacttatc tgctaagaac agctcggtat catccggact
     7621 gaactggtga ataatggctt tcttaatata aatatccata aaagttttag tcctcgtata
     7681 atgggaaggc atctgtcaat tctttgactg cacttctcac ttcttctaat acagcctcat
     7741 tttctgaatt cttaagggtt ttaatgatga gttcagccac tttgcgactt tcttcttcac
     7801 caaatccacg tgcagtaata gctgctgctc cgatacgaat cccacttgtc ttgaatggcg
     7861 acaaggtttc gtaagggatt gaatttttat ttagggtaat attgacttca tccagcaagt
     7921 tttgagcaac ttttccgttt tctacaacct tagtcacatc aacaaggaag agatggtttt
     7981 cagttccacc tgaaataata cggaaatcag ggtcttgcaa gaagacatct gccatagcct
     8041 tgctgttctt aattacattg gcagcatatt ccttgaaggc tggatccaaa acttctttga
     8101 aggaaactgc cttagccgcc acaacatgct ctaaaggacc gccctgaata cctgggaaaa
     8161 tagctgaatt gattttttta gcaagttctt cgtcattggt caaaatcaaa ccaccacgag
     8221 gtccacgaag ggttttgtgg gtcgttgttg ttgtgatatg agcgtatgga actgggcttg
     8281 gatgaaggcc agccgcaacc aaaccagcga tatgggccat gtccaccatg agcttcgcac
     8341 cgacagcatc tgcgatttca cggaattttg aaaaatcgat aatttgagaa tatgctgaag
     8401 caccagctac aatcagtttt ggttttactt cttgggcttg tttcaagata gcatcaaagt
     8461 ctaagagttc cgtttcagga tcaacactat aagaaacaaa gttgtaggtt tgaccagaga
     8521 agctaacagg agccccatga gtcaagtgtc cacctgctgc caaatccatt cccataaccg
     8581 tatcacctgg ctcaatcaag gacatgtaag ccgcacagtt agcttggctt cctgaatgtg
     8641 gttggacatt ggcaaattta gcaccgaaaa tttcttttgc gcgttcaata gcaagagtct
     8701 ctacaacgtc tactacatca gttccaccat aataacggcg tcctgggtaa ccctcggcat
     8761 atttatttgt caagatagac ccttgagctg ccataacagc cttggaaact acgttttccg
     8821 aagcaattaa ctcgatatta ttttgttggc gttcttcttc tttggcaata gcattccaga
     8881 gatcagcatc atatgcttta aaatcatctt tgtcaaaaat cataggtctt ctcctttatt
     8941 gtgtgactag tccattagtt tgattttaca ataagaaaat caaactaaaa gatgcgaata
     9001 aaccgtttct gcattttatc acaagtatag ccaacttttt cataaaatgc atgagcaccc
     9061 agacgatgat tggcagaatt taagcggata aacccataac cacatctttt tgcttcttct
     9121 tccaaccctt gtagtaaact tttaccaata ccttgacctt gcgcttgagg tgaaactgct
     9181 aaagctaaga tattaaatcc tgctttggaa tagagtgatt cgtaaacttc agcgtggaca
     9241 tatccaagta agacatgatt agctgcatcc tcatagccaa gtaggaaatg atgggaatcc
     9301 tgagacagtc tagctagttg gctagccgtt tcctctggac taaaagtata acccaaagcc
     9361 tcttggttga tgtcacatat cgctttcaca tctgtttctt gcaaatctct tagcatttca
     9421 agcctcctca aaagaaatct ctggcaaccg agcaagaata tcttctcgtt taattgcgcc
     9481 ttggcgtaag attttcacct tgtctccaga caaatccaca atagttgaat cctgtccagt
     9541 tagaaaagca tcgtcttcca gacccagaac ctcttggtca aaatccttca gaatttgttc
     9601 aaaggttaca ccacttgcct gacctgagat attggcagac ggcccaatca agggacctgt
     9661 ctctcgaatt aaatccagtg tgatagggtg actgggcatc cgaaatccaa tagttgcaag
     9721 gtcagaattt acccaatagg gaactcggtc attggcttcg agaataatgg tcaagggacc
     9781 tggcaaaaag gtctctacaa gtttttgtag ataagttggc tgattcttag aaaagtgcaa
     9841 gatatcctcg aaagaggcga tattgagatt gagtgcctta tctctaggac gacgtttgag
     9901 ttggtaaaca tggtcaactg ctttttcatc taaggccttg gcaaaaagac cataaacagt
     9961 ctctgtaggt agaacgacag ctccaccatt ttccaactct tgtctaatcc tgtccatcat
    10021 caaccacaac catcctattt tgaccaaatt ggtctttgag tgttcgaact tttttttcag
    10081 gaagatgttt tctaaaaagt tcgggaacac tttgaccttg cttgtatcca atttcaaggt
    10141 aaatcttacc accatccttg agatagtctt ttgcatcttc cgcaattcta cagtaaatag
    10201 ctaggccatc ctcgtctgca aagagagcta gatgaggctc cgaatgcaaa acattcaaac
    10261 ctacctctga ctcatcttca cgagagatat agggtggatt ggatacaatt atatcatatt
    10321 tttcagaaat ttctgcaaaa caatcagatt ttttaaaaaa tatattaaga ttttgatcgc
    10381 tagcattctc tgatgcaagc tctaaggcct cttgagaaac atctgctgct gtcactgacc
    10441 aatctggtct attttttgct agagcgagag caatagctcc actacctgtt ccaatatcca
    10501 gaactgagag attcgtctca agattttcag ttagaataag ctccactaac tcctctgttt
    10561 ctggacgagg aatcaaaacc cgttcatcca cctttaaatg cattccataa aaatctgcct
    10621 gtccaatgat gtactgagct ggcttgtgag ctgctagttg ctggtaaata tcttctacaa
    10681 attgtttttc ttcctctgtt gtcacctcct gctggagggc aaaaataaag tctgtaaaag
    10741 atagattttt cagactacga tagacaaaag agaggctttc cgcttcctct ccttgtctta
    10801 tcaactcttc ttcaaaattt gaaaataatt gagctaattt cattatttgt ttaattcttc
    10861 tagtttttgt gtttggtcat aaagcaccaa ggcatccaca acttcgtcca atttaccaga
    10921 caaaatcgta tctagttttt ggagggtcaa gccgatacgg tggtctgtga cacggttttg
    10981 tgggaagtta taagttcgga tccgttctga acggtcacca gtaccgattg tcgacttacg
    11041 ctcagcgtct tgttcatcct gagcaatctg agcaaagtgg tcagcgacgc gtgcacggat
    11101 aatcttcata gccttctcgc ggttcttctg ctgggtacgt tcttcctgca tctcaacctt
    11161 gatattggtt ggcaagtgaa cgatacgaac ggcagtcgca accttattga cgttctgtcc
    11221 accagcacca gaggcgtgat agatgtcgac acgaaggtct tttggatcaa tgtcgtattc
    11281 aacctcttca acttctggca taacaagaac tgtcgctgtc gaagtatgaa cacggccttg
    11341 gctttctgtc acaggaacac gttgcacacg gtgggcacct gattcatact taagcttaga
    11401 gtatacagac tgacctgaaa ccatagcaac cacttcttta aaaccaccga caccattcat
    11461 agaggcttcc atgacttcaa agcgccaacc ttgggcttcc gcatactttt ggtacatagt
    11521 tagcaaatct ccagcgaaaa gtgccgcttc gtctccacca gctgctccac ggatttcaag
    11581 gatgatattc ttgtcatcgt ttggatcctt tggaaggagc aaaattttca gtttttcttc
    11641 atattcttct ttttcagcct tggcatcttt gagttcttgc ttggccattt cttccaagtc
    11701 cgcatctccg cctgattcct taatcatctc ttcggcatcg acgatatttt gaaggacttg
    11761 tttatactca cggtaggcta ttacggtgtc acgattggaa gcttcttctt ttgaaagctc
    11821 cataaaacgc ttggtgtctg aaacgacatc agggtcactc agcaattctc ctagttcttc
    11881 ataacggtct tctacagctt gtagttgatc atagatgttc atttttcttc attctcctta
    11941 ttgatttcag gggcaaaata atgtttacgg caaaccgaga tataggtttc attaccacca
    12001 atctggatct gttctccatc gtaaacgggc agtccatcct gtgttcgcaa caccatggtc
    12061 gcctttttct tgcaatactg acagatggtc ttgatttcgt caatcttgtc tgctaaaagc
    12121 aagagatatt tggaaccttc gaacaattca ttgcgaaagt catttttcaa gccaaaagcc
    12181 atgacgggta tgtctaactc gtccacaaca cgagctaggt cataaacatg gtggcgtttg
    12241 agaaactggg cttcatcgac caaaacacag taaggttttt ctggtaggtc tcggatatag
    12301 ccaaagatat ccgttgtttc ctcaatcgca agggcagggc gtttcatgcc aattcgactc
    12361 gacacatagc caacaccgtc acgcgtatcc agagccgagg tcataatcac aacacctttt
    12421 ccttgctcct cgtagttata ggccactttg agaatctcaa tcgttttacc agagttcatg
    12481 gtcccataac gatagtacaa ctgtgccatg tttcttgctt cacgtccatt tctaaatttt
    12541 tgctacattc tagtatatca taattttctt aagctttaaa cggcaaaatg tggtaaaatg
    12601 gaagaaatca aaactagtgg aggaagctat tatgccattt gtacgcatcg atttatttga
    12661 aggacgcacg ctcgagcaaa agaaagctct tgctaaggaa gtaacggaag cggttgttcg
    12721 caacactgga gcacctcaat ctgctgttca tgtcatcatc aacgacatgc cagaaggaac
    12781 ttatttccca caaggggaaa tgcgcaccaa ataagctagc ttaagcagaa ttgcttaggc
    12841 tttttcaatc tccaagtagc acccattgaa gaaatagtct aaatttgtta caatttgaaa
    12901 agagacttgg aaaattccaa gaaaagagca attaattaaa ggaaacatta tgattacacg
    12961 tgaatttgat accatcgctg ctatctctac tccactaggt gaaggggcta ttggtattgt
    13021 ccgcctgagc ggaacagaca gttttgctat tgtgcaaaag atttttaaag gaaaagactt
    13081 gaacaaggtt gccagccaca ctctcaacta cggtcacatt attgatcctc tgactggtaa
    13141 agtcatggac gaggttatgg ttggggctat gaagtctcca aagaccttca ctcgtgagga
    13201 tattatcgag attaacaccc acggtgggat tgcggtgacc aatgaaattc tccagctagc
    13261 tattcgtgaa ggggctcggt tggcagaacc tggtgaattt accaaacgtg cttttttaaa
    13321 cggtcgcgta gacttgacac aggcagaggc tgtgatggat atcatccgtg ccaagactga
    13381 caaggccatg aacattgcgg tcaaacaatt agacggctcc ctttctgacc tcattaacaa
    13441 tacccgtcaa gaaatcctca atacacttgc ccaagttgag gtcaatatcg actatcctga
    13501 atatgatgat gttgaggaag ctactactgc cgttgtccgt gagaagacta tggagtttga
    13561 gcaattgcta accaatcttc ttagaacagc ccgtcgcggc aaaatccttc gtgaaggaat
    13621 ttcaacagct atcatcggac gtcccaacgt tgggaaatcc agccttctca acaacctctt
    13681 gcgtgaggac aaggctatcg taacagatat cgctgggaca acacgagatg tcatcgaaga
    13741 gtacgtcaac atcaatggtg tacctctcaa attgattgat acagccggta ttcgtgaaac
    13801 agatgacata gtggaacaaa tcggtgttga gcgttcgaaa aaagccctca aggaagccga
    13861 cttggttcta ctagtgctaa atgccagtga acaactgact gcgcaagaca gacaacttct
    13921 tgaaattagc caagatacca atcgcattat tctacttaat aaaaccgacc tgccagaaac
    13981 gattgaaact tcgaaactac ctgaagacgt tatccgtatt tcagtcctta aaaaccaaaa
    14041 catcgacaag attgaagagc gaatcaacaa cctcttcttt gaaaatgctg gcttggtcga
    14101 gcaagatgct acttacttgt caaacgcccg tcacatttcc ctgattgaaa aagcagttga
    14161 aagcctacaa gccgttaatc aaggtcttga gctggggatg ccagttgatt tgcttcaagt
    14221 tgacttgact cgtacttggg aaatcctcgg agaaatcact ggggatgctg ctccagatga
    14281 actcatcacc caactcttta gccaattctg tttaggaaaa taagaaaaat ccatgatcct
    14341 tcattcggtc atggatttta ttgtctttat tagtaatctg gtcttaagac ccctgttaca
    14401 gttgccttag ttgcttcgta gtcgccatct acgacaacct tgataatgcg tttggcatct
    14461 tcttctggtg ctggaacaag aggtagacga gtgggtccag cttcaaatcc catatagtta
    14521 agaattgcct taactggagc aggacttgga taagagaaga gagcattaac cttaggaatg
    14581 aatttacgct gaattgctgc ggctttcttc atatcgcttt ctgcaatggc agtaaacatc
    14641 tcgtgcattt catccccatt tgtatgagag gcaacagaaa taaccccatc cgccccaagg
    14701 ttcatggcat ggaaagcatc tccatcctca cctgtataaa tcaagaactc ttcaggcttg
    14761 tgctcaatca agtaagccat attagccaag ctagtacatt ctttgacacc gataatattt
    14821 ggatggtcag ccaagcgaag catggtttct ggagtcaatt cgaccactac acgccctgga
    14881 atgttataga taataattgg taggtcagaa gcatctgcaa tagccttaaa gtgctgatac
    14941 atcccttctt gagaaggttt gttgtagtaa ggaacaatag caagcccagc tgcgaaacca
    15001 ccaaattccg ctacttcttt gacaaactca atagagtcac gcgtatcatt agtacctaca
    15061 cccgcaatca aaggaacgcg tccattgaca accttttgta cagccgcaaa caactccaac
    15121 tcctcatcgt gggtcaaagt tggactctca gcagtcgttc ctgcgagaag aattccatcc
    15181 gtatgatggg ccaataaatg ctcaatcaag gctggaatag catcaaagtt aatggaacca
    15241 tcctcatgga agggggtaat aaaggctgta atgattttac attcttttaa atcttgataa
    15301 gacacgaaaa cacctctcta tttcaaagaa ggagttcatc tgaactccta aacgatatga
    15361 ctattttaat tcaaatttca attcagctgt tggacgaacc aatccacgtt catgaagagt
    15421 ttcagcaatc tgaactgagt tccaagcagc acctttgaga aggttatctg aaacaaccca
    15481 catgtgaatt cctttttctg catccaagtc tttacggata cgaccaacaa aggtatcacg
    15541 tgaaccaact gcattgatgg cttgaggata gatttgatga actacatcat cttcaagaac
    15601 agcacctggg aaggatgcga tagctgcttt gacttcttca attggagcca cttcctttgt
    15661 ttcaatataa actgactcag agtgagctga caagactgga atacgcacac atgtcgcaga
    15721 tactgcaatg ctatcatctt ccataatttt cttagtttcc ttggtcatct tcatctcttc
    15781 gtacgtgtaa tcattatcag tgaagacatc aatttgtgga agagcgttaa aggcgatagg
    15841 ataatgtttc ttgtcaccac ctgaaggcaa gatttccgca tgcaaatcac gtggtttcac
    15901 tccatcattc aagacctcac gaagttcacg ttgtgtctca agaattgctc ccataccagc
    15961 acctgaaacg gcttgataag ttgaaacaat gatacggtcc aagccccatt tttggcgaac
    16021 cggctcaaga gccaccatca tttggattgt tgaacagtta gggcaggcaa tgattccgtt
    16081 gtgggcatca agtgcatgag cattgacctc tggaacaacc aaaggaacat ctggattttg
    16141 acggaaataa gatgtattat ccactactac cacgccagct tttactgcgt atggtgcata
    16201 cttagctgat gtcgaaccac ctgctgaaaa gagagcaata tcaactcctt caaaagctgt
    16261 ttcagtcgtt tcttcaatcg taatatcttg atctttaaat ttcaatgact tacctgctga
    16321 acgtgcagaa gcaaggtaac ggattttatc gattggaagt gttgattctt ccaacatttt
    16381 tatcatctga gcaccgacag cacctgtcgc gccgactaca gcaactgtat atcccataaa
    16441 taacctcttt cggaattttc taaaaatttc tataatagaa gtattatact actttttcca
    16501 tgaattgcaa agctttttca tcattttttg gaaaataaaa atccccaatc gctagactag
    16561 ggattaagag gcatcttcat gtgatgagca ggttcacaca actcatcaag gtccgctcct
    16621 gcgttatgac ctccttatgc tcaatagtag cccgaagact acctatcgac tcatcgcatc
    16681 tactattcta ctaaagagaa tgacttttgt caacagacga aactctttat actcttcgaa
    16741 aatctcttca aacttcgtca gcgtcgcctt gccgtatgta tggttactga cttcgtcagt
    16801 ttcatctaca acctcaaaaa catgttttaa gctgacttcg tcagttctat ctacaacctc
    16861 aaagcagtgc tttgagcaac ctgcggctag tttcctagtt tactctttga ttttcattga
    16921 gtattagttt tatttacaca ggtgcataag aaatatattg gaaaaattgg gggcagacat
    16981 aacaaaaacc cttgtaaatc aagggctttt ctgtctattt aatgctaatt gccgggattg
    17041 aaccggcgac ctcatcctta ccatggatgc gctctgccaa ctgagctaaa tcagcttact
    17101 taagaagtat actatattca gaaaaacttg tcaagtttcc ttagaaattt tccataggaa
    17161 aaagccaggt aagaactacc tagcttatct tcttctattt acttaaatca attcgacgac
    17221 caattttacc aataataatc gctccaataa tcaatgaagc aaattcaccg attcctgttg
    17281 taaaccaagt aaggaagaat ggtaattctg ctacaatatt taactctgca gcgatagtaa
    17341 acatggaaat tgaaaagagg attgaaaaga agaaatgatc ttttcgaatc aatccgttaa
    17401 agaggtaatc cttgctatat tttgcaaaaa gccaaacacc taaactgagg aatactaagg
    17461 ttgaaccacc accaacaaag acatctagca gtccgaagct aaagaaatta gcaatcatac
    17521 aaccgatggt aactccgatg atgtacttag gattgtaaaa agccataaag ttcatcattt
    17581 ctgaaatacg aaactgataa gcaccatagc tgatggcatt taggggcggt gtgacagtca
    17641 aaacgacgta gatagcagcg acgattgcaa tatctgcaac atcacgaata gttaattttt
    17701 tcatcttttc tcctttggcg gttatccgcg tgtaatatgc ttggtgaaag aagctaagca
    17761 ccaagggttg attcattcaa ccttactagt atagcacaat agcctgcttt atgctatact
    17821 taaagcatga aaaaacctat tcaaaaattt tttaacaatg aaatcttagc ctatctcttt
    17881 tttggccttg cgacaactct agtttcgatt ctctcacgac tagtcattta tcaactaagt
    17941 catcaggaac tccttgctac ggccttggca aatattatcg gtatcttatt tgcctttatc
    18001 acaaatgata cgattgtatt taagcaaaaa aagaaaaata gacttatccg tttagtgaaa
    18061 ttttctcttg ctcgcctttc tacttttctg ttagacttgt ttttaacttt tctctttgta
    18121 acccaatttc ctcacatcat agggcaattc gtaactgaaa acattgataa gataaatgct
    18181 attgaaacaa taattgcaca aatattcatt attatcatca attatatctt gagcaaggtc
    18241 tatattttta gaaaataatg ctccatttat gagcctttta attgccttct ataatgattt
    18301 aggatacaat gaaacacgaa acattgaaag gaaaacagta tgttaaaaaa tctaaaatcg
    18361 ttcttgcttc gaggaaatgt tattgacctt gctgtcggtg ttgtcattgc ctctgctttt
    18421 ggtgctatcg ttacttcact tgtaaacgac attatcactc ctcttatttt aaatcccgct
    18481 ttgaaagctg ctaaagtcga acgtatcgct caactttctt ggcatggagt cggctatggt
    18541 aactttttaa gtgctattat caattttatc tttgtgggta ccgccctctt ctttattatc
    18601 aagggcattg aaaaagcaca gaaactgact ggcataaaga aagaaaaaac tgccgaaaaa
    18661 aaaccaaccg aattggaagt ccttcaagaa ataaaagctc tccttgagaa aaaataatcg
    18721 attaaaagga tctagcgcaa agctaaatcc ttttttcttt actctttggg taacttgcct
    18781 gctttttcaa gcattttttt gataagataa ggcatcttaa cattctcccg cccttttttc
    18841 tcaatcagtt ttttcacaaa ttctggcatt tgtaaatctt cagattcagc taaaatgttt
    18901 ttcgtctcgt ctgaaggaac taactcatca aaggtctctt cttctgggag aaattcctta
    18961 aactcttgat tttcattgac tctaactgta ttgaccaaag catcaatcaa acgagaatct
    19021 gtatttggca ttggtggacg atggtagttt accccaaatt cctggcataa gtcataacat
    19081 tctacatcat tgtcaaacaa gacctcaata tgctcactga tgaaactaat aggtacaaaa
    19141 atataatggt ctggatgttc tgtctgttct ctgagatact ccaaaacatc tggtttaatc
    19201 catggaatac caatatcact ctcactctgc caagtgttgg tatattgttc tgaactcaag
    19261 cccagttttt cagcgactag cttgctattt tcgaaaattt ggtctatata aggatcacca
    19321 aagtccaagg caaaaatggg cacactatgg gctgaaaaga tgactttaaa gctgtcttgc
    19381 tttacttctt cttttaaaat cttagcaatt tcatctgccc aatagtttaa gagcgcttct
    19441 tcctgatacc agtccttaat gaccaaaaat tgaatttgtt tgctttctaa aaatttttca
    19501 tagcccatga cagagtagaa agaataatgg ggctccaaaa tcaagcaaat acactgttca
    19561 attccgtctg cttccatttg accgatcaca tctggaataa agggactgga aaacttatta
    19621 gcaaagtaga cactgtattc attccccagc ctagcttcta ccaaggcaac ctcttgacgg
    19681 gtaatttttt gcagaggcgt gcctccaatt cgtacataat tatcatagag agtctgaatc
    19741 tcgtggtctt ggggtctcac cccacgacga atatttgtga aaaaatcagc tacaccttca
    19801 aaggtaatct cttctggtga accgaaggtc atcattaaaa ttgctttttt catgactgct
    19861 tccttgtgat gtttttatca agtttatttt atcattaatt aatcgataag taaacgctaa
    19921 tatagggaca ttctcaatct tctgtctttt gtttttctct tctttctatg atacaatgga
    19981 aaaaataaat tcaaaaggag tttttttatg acttatccaa atctcttgga ccgcttctta
    20041 acctacgtta aggtcaacac gcgctctgat gaacactcta ctactactcc aagtacacag
    20101 agtcaggttg acttcgcaac aaatgtccta attcctgaaa tgaaacgtgt tggactgcaa
    20161 aatgtttact atctaccgaa tggttttgct attggaacct tgccagccaa cgatccgtct
    20221 ttaacacgta agattggttt tatatcgcac atggatactg ctgattttaa tgctgaagga
    20281 gtcaatccac aggtaattga aaactacgat ggtggtgtga ttgaactagg gaattctggt
    20341 ttcaaactcg atccagctga cttcaagagt cttgaaaaat atccaggaca aacgctcatc
    20401 acaacagatg gaacaagctt gctaggtgct gatgacaagt caggaattgc tgaaattatg
    20461 acagccattg aatatctaac tgctcatcct gaaattaagc actgtgagat tcgtgttggt
    20521 tttggtccag atgaagaaat cggtgttggt gccaataaat ttgatgcaga agattttgat
    20581 gtggattttg cctacactgt tgatggtggt ccactaggtg aacttcagta cgagactttc
    20641 tcagccgctg gtgctgaatt gcatttccaa ggccgtaatg tccaccctgg tactgccaaa
    20701 gagcagatgg tcaatgccct tcagctagca attgattttc ataatcaact tccagaaaat
    20761 gaccgacctg agttaactga aggttaccaa ggtttttacc atctaatgga tgtgacaggt
    20821 agtgttgagg aggtgcgtgc aagctacatc attcgtgatt ttgaaaaaga tgcctttgaa
    20881 gcgcgtaaag catccataca atctatcgct gataagatga atgcagaact tgggagctac
    20941 cgtgtcactc tcaacttgac agaccagtac tacaatatga aagaagtcat tgaaaaagat
    21001 atgactccaa ttaccattgc taaagccgtt atggaagatc taggtatcac gcctattatc
    21061 gaaccaatcc ggggtggaac agacggctct aagatttcct ttatgggaat cccaactccg
    21121 aatatctttg caggtggcga aaatatgcac ggacgttttg aatacgttag ccttcagact
    21181 atggaacgtg cagttgatac catcattggc atcgtagctt ataaaggcta aaaagacgag
    21241 gtagctcagc tacttcgcct ttctttttat tctactggtt tttcttgatt tccagtagtt
    21301 gtagaagatt ctgttgtttc attttctgaa gttgattcag caggtttaga atctcttgta
    21361 ttgcttggtt tgttttcgtc gctagcaatt tcaatgttag attctgcagt tgcgtttggt
    21421 tggttctcag cactggtgtt atcactattt gcttcagcat ttcttgctgg acttgtttct
    21481 tcacttgcgc tagcttttga ctggatttga tgattcaaaa ctagaatagc ttttgtcgat
    21541 tcaagtaaag ctgttttgtc tttactctta gcagaaagtt gatctaataa tgcatccacc
    21601 ttatcaaagt ccgcatcaga tccattatta ctttctaaat aagagtgaag cgacatgaga
    21661 atatcgtaga gtttttgata gagtacaagt gtctgagaat cttgctcagc attttccttt
    21721 tcttgttgaa gggcgctagc gatacgagtc aagacatctt ttacctgact gtttacttca
    21781 tccaagtctg catcagcctt gtttgtggca gcttttagat tttctacttc ttctgccaag
    21841 gattgtctga ttccttcttc atggatttgt tccaagagtt gatttacctt gctcaaaaga
    21901 ctttctactt cttccttgct atctgtcgca gattattggt tgccatctac catgtactcc
    21961 taaaacagga gagttataat ccaagattac aaggccttac agaaataaga aatccagata
    22021 agacaatgtt cgtccaagac gctattcgct tcgcacagca gcacggattc aatatgcttt
    22081 aattttaaag tttaggtgtc aagacctctt tttagtgtgc ccaaaattta gagaagtaat
    22141 caatcaacta acttttattt ttttcaaact ttcagtaaac tgacctaaag ctaactcaat
    22201 ctgtctttgt agatgcttct gctatcagct agaagttgat ctacttttgc caagactgcc
    22261 ttctcatcaa aagttccagg ttgatagttg gattgcaggg atggaatctt gtttttcaaa
    22321 gccgcttcat atcccttagt ttgaaccttg atgtagtgat tgtggtcgcc atgaggaatc
    22381 acaaaacctt ctgaatcttc acttataatt cgattggcat caaaaccatg accatcttct
    22441 tcctcatggt ggacatgtag tgacggatta cttaatacag aactagaaga acttcctgcc
    22501 tcttccgtgt tagagtgtga tgggggattg ttaagagatg acttaggaat ataatgatag
    22561 tgatccccgt gtcttactat ataagcatca cctgtatctc tgacaatatc attagggtta
    22621 aagacataac catcatctgc tgcagaaaca ccattattcg gtgtcaccga caaagattga
    22681 ctgagagctg tagtattccc tgataattat acttttgcag ctgctaattc acctgccgac
    22741 aagtcactct caggaatgaa atgatagtga ccaccatgtg gtactatagt aggttgaaat
    22801 agaatatgag caaattgata aggggatttt aaagtaattt ctaacaatga tttagaaact
    22861 atgatgtgct attctaaatt caactcacta tatataacca tcatcggtag tataacgtcc
    22921 ctgtaatttt gctacagata cttctgcact agctccttta tcgtctttac catgttcttg
    22981 tttttggcga ttgatttcat cttttgttcg tacattttct gcatgagctt gatctttaag
    23041 gtaaacataa tactttccat ctaccttaat aatatatcct cccttaacct aactgacgat
    23101 atcttgatct ttcggctgat agttgggggc tttcattaat agctcttcac taaagagcgc
    23161 atcaaaagga actttaccat tatagtagcg ataatgatcg ccatgagaag ttacataacc
    23221 ttgatctgta atcttaataa caatttgttt tgcttgaatt ccttcttttt gactaaccta
    23281 gtctggagtc aaattttcag tcttcttagt gtctttatta ctgtttacat atgaaacacg
    23341 atttttatct gtattggcct gttagctatg ttggttcaga gtataaacac acagacttaa
    23401 ggaaaggata acaac
//
LOCUS       DAVMGZ010000017        23234 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000017 DAVMGZ010000000
VERSION     DAVMGZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23234
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.018"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            74..262
                     /locus_tag="VYQ93_000563"
     CDS             74..262
                     /locus_tag="VYQ93_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335301.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSWSRYDSYSEYKVG
                     GGWNYARYEVINYYSGGY"
     gene            295..882
                     /locus_tag="VYQ93_000564"
     CDS             295..882
                     /locus_tag="VYQ93_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3335302.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            886..2070
                     /locus_tag="VYQ93_000565"
     CDS             886..2070
                     /locus_tag="VYQ93_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3335303.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASPGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKEFFN
                     RF"
     misc_feature    2234..2365
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2381..2911
                     /gene="infC"
                     /locus_tag="VYQ93_000566"
     CDS             2381..2911
                     /gene="infC"
                     /locus_tag="VYQ93_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV3335304.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            2944..3144
                     /gene="rpmI"
                     /locus_tag="VYQ93_000567"
     CDS             2944..3144
                     /gene="rpmI"
                     /locus_tag="VYQ93_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV3335305.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            3196..3555
                     /gene="rplT"
                     /locus_tag="VYQ93_000568"
     CDS             3196..3555
                     /gene="rplT"
                     /locus_tag="VYQ93_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV3335306.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            3613..3993
                     /locus_tag="VYQ93_000569"
     CDS             3613..3993
                     /locus_tag="VYQ93_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV3335307.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            4243..5043
                     /locus_tag="VYQ93_000570"
     CDS             4243..5043
                     /locus_tag="VYQ93_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV3335308.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            5006..5992
                     /locus_tag="VYQ93_000571"
     CDS             5006..5992
                     /locus_tag="VYQ93_000571"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV3335309.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            6034..8067
                     /locus_tag="VYQ93_000572"
     CDS             6034..8067
                     /locus_tag="VYQ93_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV3335310.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMTSD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(8128..9783)
                     /gene="pavA"
                     /locus_tag="VYQ93_000573"
     CDS             complement(8128..9783)
                     /gene="pavA"
                     /locus_tag="VYQ93_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV3335311.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            9888..10385
                     /gene="ybeY"
                     /locus_tag="VYQ93_000574"
     CDS             9888..10385
                     /gene="ybeY"
                     /locus_tag="VYQ93_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV3335312.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            10366..10761
                     /locus_tag="VYQ93_000575"
     CDS             10366..10761
                     /locus_tag="VYQ93_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV3335313.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENMVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            10778..11677
                     /gene="era"
                     /locus_tag="VYQ93_000576"
     CDS             10778..11677
                     /gene="era"
                     /locus_tag="VYQ93_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV3335314.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            11725..12549
                     /gene="mutM"
                     /locus_tag="VYQ93_000577"
     CDS             11725..12549
                     /gene="mutM"
                     /locus_tag="VYQ93_000577"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV3335315.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            12549..13154
                     /locus_tag="VYQ93_000578"
     CDS             12549..13154
                     /locus_tag="VYQ93_000578"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV3335316.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEAIVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            13141..14340
                     /locus_tag="VYQ93_000579"
     CDS             13141..14340
                     /locus_tag="VYQ93_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEV3335317.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRIFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            14340..14489
                     /gene="rpmG"
                     /locus_tag="VYQ93_000580"
     CDS             14340..14489
                     /gene="rpmG"
                     /locus_tag="VYQ93_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV3335318.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            14529..14762
                     /gene="secG"
                     /locus_tag="VYQ93_000581"
     CDS             14529..14762
                     /gene="secG"
                     /locus_tag="VYQ93_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV3335319.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            14864..17218
                     /gene="rnr"
                     /locus_tag="VYQ93_000582"
     CDS             14864..17218
                     /gene="rnr"
                     /locus_tag="VYQ93_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV3335320.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            17181..17648
                     /gene="smpB"
                     /locus_tag="VYQ93_000583"
     CDS             17181..17648
                     /gene="smpB"
                     /locus_tag="VYQ93_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV3335321.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            17664..18524
                     /gene="tehB"
                     /locus_tag="VYQ93_000584"
     CDS             17664..18524
                     /gene="tehB"
                     /locus_tag="VYQ93_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV3335322.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            18597..19550
                     /gene="coiA"
                     /locus_tag="VYQ93_000585"
     CDS             18597..19550
                     /gene="coiA"
                     /locus_tag="VYQ93_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV3335323.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKGKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            19569..21371
                     /gene="pepF"
                     /locus_tag="VYQ93_000586"
     CDS             19569..21371
                     /gene="pepF"
                     /locus_tag="VYQ93_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV3335324.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYNFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            21373..22086
                     /locus_tag="VYQ93_000587"
     CDS             21373..22086
                     /locus_tag="VYQ93_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV3335325.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            22153..23094
                     /gene="prsA"
                     /locus_tag="VYQ93_000588"
     CDS             22153..23094
                     /gene="prsA"
                     /locus_tag="VYQ93_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV3335326.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
BASE COUNT         7043 a         4282 c         5208 g         6701 t
ORIGIN      
        1 ttaaaaatca gcaaaagttg ttttattagt tagtttataa tctattggtc ttcttcagtc
       61 cagtgtatct gctgtgacag tcactaaaag ttacaagtat gattggaata cggtttggga
      121 atatagtacc aactatcacg accatcagta tgcttggatt ccgtcatggt ctcgttatga
      181 cagctattct gagtataaag ttggcggagg ctggaactac gctcgttatg aggtcataaa
      241 ctattacagc ggaggctatt aattcttaaa gagtgagaaa aaggagggct agatatgttg
      301 cagcttactc atgtgacctt aaaaacgcga caagtcatct tgcaagatgt ggatttcacc
      361 tttaaaaagg gtagggttta tggtcttctt gctatcaatg gctctggaaa gacgaccctg
      421 ttccgtgcca ttagcaattt aattcccata agtagtggaa atatcgcagc ccctccttct
      481 ttattttatt atgagagtat tgaatggctg gatggaaact taagtgggat ggactacctt
      541 cgtcttatca aaaacatctg gaagtcaggt ctgaacttga gggatgaaat cgcctattgg
      601 gaaatgtctg actatatcag tcttcccatt cgcaagtatt ccttaggcat gaagcaacgc
      661 ttggtgattg ccatgtattt cctcagtcag gccaaatgct ggctcatgga tgagattaca
      721 aatggcttag atgagtatta tcgacagaag ttttttgata ggctagcaca aatcgataga
      781 caagaacagc tggttctttt aagttcccac tataaggaag agttggttga tgtctgcgat
      841 agagtagtaa ccattcatca ggggcagata gaagaggttt agtttatgaa agatgttagt
      901 ctatttttat tgaaaaaagt tttcaaaagc cgcttaaact ggattgtctt agctttattt
      961 gtatctgtac tcggtgttac cttttattta aatagtcaga ctgcaaactc acacagcttg
     1021 gagagcaggt tggaaagtcg cattgcagcc aacgagaggg ctatcaatga aaatgaagag
     1081 aaactctccc aaatgtctga taccagctcg gaggaatacc agtttgctaa aaataattta
     1141 gacgtgcaaa aaaatctttt gacgcgaaag acagaaattc taactttact aaaagaaggg
     1201 cgctggaaag aagcctacta tttgcagtgg caagatgaag agaagaatta tgaatttgta
     1261 tcaaatgacc cgactgctag ccctggctta aaaatggggg ttgaccgcga acggaagatt
     1321 taccaagccc tgtatccctt gaacataaaa gcacatactt tggagtttcc gacccacggg
     1381 attgatcaga ttgtctggat tttagaggtt atcatcccaa gtttgtttgt ggttgctatt
     1441 atttttatgc taacacaact atttgcagaa agatatcaaa atcatctgga cacagctcac
     1501 ttatatcctg tttcaaaagt gacatttgca atatcctctc ttggagttgg agtgggatat
     1561 gtaactgtgc tgtttatcgg aatctgtggc ttttcttttc tagtgggaag tctgataagt
     1621 ggttttggac agttagatta tccctaccca atttatagct tagtgaatca agaagtaact
     1681 attgggaaaa tacaagatgt attatttcct ggcttgctct tagctttctt agcctttatc
     1741 gtcattgtgg aagttgtgta cttgattgct tactttttca agcaaaaaat gcctgtcctc
     1801 tttctttcac tcattgggat tgttggctta ttgtttggta tccaaaccat tcagcctctt
     1861 caaaggattg cacatctgat tccctttact tacttgcgtt cagtggagat tttatctgga
     1921 agattaccta agcagattga taatgtcgat ctaaattgga gcatgggaat ggtcttactt
     1981 ccttgcctga ttatcttttt gctattggga attctattta ttgaaagatg gggaagttca
     2041 cagaaaaaag aattttttaa tagattctag ctttcctata ggtagggaaa ataagtaaaa
     2101 actaacatag agagggaatc aacttgattc tctctttttg attcgaaaac caaaccaaaa
     2161 tacaaacaca aacttttcaa aaaataactt tttatcttga caagagctag aaaacttggt
     2221 atcatataaa agttgagaaa agcagaagtg agagcttctc gccttgtgac attaagttgc
     2281 ctggccctac ggatgaaaag tttcgaagaa acgctatcat aacgtgcggg cttgtatatt
     2341 tacaagtccg ctattgtttt tctctaataa aacaaaagag gtgaaaacca tagcaaagca
     2401 agacttattc atcaatgatg agattcgtgt acgtgaagtt cgcttgattg gtcttgaagg
     2461 agaacagcta ggtatcaagc cactcagtga agcgcaagct ttggctgata acgctaatgt
     2521 tgacctagta ttgattcaac cccaagccaa accgcctgtt gcaaaaatta tggactacgg
     2581 taagttcaaa tttgagtacc agaagaagca aaaagaacaa cgtaaaaaac aaagcgttgt
     2641 tactgtgaaa gaagttcgtc taagtccaac tattgacaag ggtgactttg atacaaaact
     2701 tcgtaatgca cgtaagttcc ttgaaaaagg aaataaagtt aaggtatcta ttcgctttaa
     2761 gggtcgtatg attacccata aagagattgg tgcaaaagtt ttagccgagt ttgctgaagc
     2821 aactcaagat attgccatca tcgaacaacg tgctaaaatg gatggacgtc aaatgttcat
     2881 gcaattggcg ccagcgactg acaaaaaata attgtcagaa agttaaataa aggagaaaaa
     2941 atcatgccaa aacaaaaaac acaccgcgca tcagctaaac gtttcaaacg tacaggttct
     3001 ggtggactta aacgtttccg tgcttacact tctcaccgtt tccacggaaa aactaagaaa
     3061 caacgtcgtc atcttcgtaa agcatctatg gtgcattcag gagattacaa acgtatcaaa
     3121 gcaatgctta ctcgcttgaa ataatagctt gactgtaagt aaacaattct aggaaatatt
     3181 tggaggaaat aaaacatggc acgtgttaaa ggtggcgttg tatcacgcaa acgtcgtaaa
     3241 cgtattctta aattagcaaa aggttactat ggagctaaac acatcttgtt ccgtactgca
     3301 aaagaacaag taatgaactc ttactactat gcataccgtg accgtcgtca gaaaaaacgt
     3361 gacttccgca aattgtggat tactcgtatc aatgcggcag ctcgtatgaa cggactttca
     3421 tactcacaat tgatgcatgg tttgaaattg gctgagatcg aagttaaccg taaaatgctt
     3481 gctgacttgg ctgttaacga tgcagtagct ttcacagctc ttgcagatgc agctaaagca
     3541 aaacttggta aataacaact gatgagactg gaacaggatt gtccagtctt ttttaaaata
     3601 aaggagaaag atatggcttc aaaaatgcta cacacttgct tacgagtaga aaatcttgaa
     3661 aaatcaatcg cattttatca agatgctttt ggttttaaag aattgcgtcg cagagatttt
     3721 ccagaccatg ccttcacgat tgtctatcta ggtcttgagg gtgatgatta tgagttggag
     3781 ttgacttata actacgatca cggtccgtat gtggttggtg atgggtttgc ccatatcgcc
     3841 ctcagtacac ctgatcttga ggcacttcat caagaacaca gtgcaaaagg ctatgaagtg
     3901 actgagccaa atggtctacc aggaactaca ccaaactatt actttgttaa agaccctgat
     3961 ggatataagg tcgaagtcat tcgtgaaaaa taatttcgtg caagttaagt agaatattcg
     4021 ttttctactt gacttttttt agctgaaaga gtatactaat aaaaattcaa cctttaaggg
     4081 gagtccagag agactcacaa ggtgtcagat aaaagaatag tgcaattttc tagaggagac
     4141 cttttgagtg tgctctcttg tgttgtacga ttttaactga ggccttgcac tagcaaggtc
     4201 ttttctttat ctggtcccct taaaatttaa ggaggaaaag ttatgaatct cacatgtaag
     4261 aagcgtttgg gtgtcattcg gttggaaacc atgaaggtgg ttgcacaaga ggaaatcgcg
     4321 ccagcaatct ttgaattagt cctagaagga gaaatggttg aagccatgcg agcaggccaa
     4381 tttcttcatc tgcgtgtacc agacgatgcc catctcttac gtcgtcctat ttcaatttcg
     4441 tctattgaca aggcaaacaa gcagtgtcac ctcatttatc ggattgaggg agctgggact
     4501 gcaatttttt caaccttaag tcagggagac actcttgatg tgatgggtcc tcagggaaat
     4561 ggttttgact tgtctgacct tgatgagcag aatcaggttc tccttgttgg tggtgggatt
     4621 ggtgttccac ccttgcttga ggtggccaag gaattgcatg aacgtggagt gaaagtagtg
     4681 acagtcctcg gttttgctaa taaggatgct gttattttga aaacggaatt ggctcagtat
     4741 ggtcaggtct ttgtaacgac agatgatggt tcttatggca tcaagggaaa tgtttccgtt
     4801 gttatcaatg atttagatag tcagtttgac gctgtttact cgtgtggggc tccaggaatg
     4861 atgaagtata tcaatcaaac ctttgatgat cacccaagag cctatttatc tctggaatct
     4921 cgtatggctt gtgggatggg agcttgctat gcctgtgttc taaaagtacc agaaagcgag
     4981 acggtcagcc aacgcgtctg tgaagatggt cctgttttcc gcacaggaac agttgtatta
     5041 taaggagaaa attatgacta caaatcgatt acaagtgtct ctacctggtt tggatttgaa
     5101 aaatccgatt attccagcat caggctgttt tggctttgga caagagtatg ccaagtacta
     5161 tgatttaaac cttttaggtt ctattatgat caaggcgaca acccttgaac cacgttttgg
     5221 gaacccaact ccaagagtag cagagacgcc tgctggtatg ctcaatgcaa ttggcttgca
     5281 aaatcctggt ttagaggttg ttttggctga aaagttacct tggctggaaa gagaatatcc
     5341 aaatcttcct attattgcca atgtagctgg tttttcaaaa caagagtatg cagctgtttc
     5401 tcatgggatt tccaaggcaa ctaatgtaaa agctatcgag ctcaatattt cttgtcccaa
     5461 tgttgaccac tgtaatcatg gacttttgat tggtcaagat ccagatttgg cttatgatgt
     5521 ggtgaaagca gctgtggaag cctcagaagt gccagtttat gtcaaattaa ccccgagtgt
     5581 gaccgatatc gttactgtcg caaaagctgc agaagatgcg ggagcaagtg gcttgaccat
     5641 gatcaatact ctggttggaa tgcgctttga cctcaaaacc agaaaaccaa tcttggccaa
     5701 tggaacaggt ggaatgtcag gtccagcagt ctttccagta gccctcaaac tcatccgcca
     5761 agtagcccaa acaacagacc tgcctatcat tggaatgggg ggagtggatt cggctgaagc
     5821 tgccctagaa atgtatctgg ctggggcatc tgctatcgga gttggaacag ctaactttac
     5881 caatccttat gcctgccctg acatcatcga aaatttacca aaagtcatgg ataaatacgg
     5941 tattagcagt ctggaagaac tccgtcagga agtaaaagag tctctgaggt aaactgcaat
     6001 caatctgttc ttgatttttt attagtttgt aatatgaatt taggagaatt ttggtacaat
     6061 aaaataaata agaacagagg aagaaggtta atgaagaaag taagatttat ttttttagct
     6121 ctgctatttt tcttagctag tccagagggt gcaatgacta gtgatggtac ttggcaagga
     6181 aaacagtatc tgaaagaaga tggcagtcaa gcagcgaatg agtgggtttt tgatactcat
     6241 tatcaatctt ggttctatat aaaagcagat gctaactatg ctgaaaatga atggctaaag
     6301 caaggtgacg actattttta cctcaaatct ggtggctata tggccaaatc agaatgggta
     6361 gaagacaagg gagcctttta ttatcttgac caagatggaa agatgaaacg aaatgcttgg
     6421 gtaggaactt cctatgttgg tgcaacaggt gccaaagtaa tagaagactg ggtctatgat
     6481 tctcaatacg atgcttggtt ttatatcaaa gcagatggac agcacgcaga gaaagaatgg
     6541 ctccaaatta aagggaagga ctattatttc aaatccggtg gttatctact gacaagtcag
     6601 tggattaatc aagcttatgt gaatgctagt ggtgccaaag tacagcaagg ttggcttttt
     6661 gacaaacaat accaatcttg gttttacatc aaagaaaatg gaaactatgc tgataaagaa
     6721 tggattttcg agaatggtca ctattattat ctaaaatccg gtggctacat ggcagccaat
     6781 gaatggattt gggataagga atcttggttt tacctcaagt ttgatggcaa aatggctgaa
     6841 aaagaatggg tctacgattc tcatagtcaa gcctggtact acttcaaatc cggtggttac
     6901 atgacagcca atgaatggat ttgggataag gaatcttggt tttatctcaa atctgatggg
     6961 aaaatagctg aaaaagaatg ggtctacgat tctcatagtc aagcttggta ctacttcaaa
     7021 tccggtggtt acatgacagc caatgaatgg atttgggata aggaatcttg gttttacctc
     7081 aagtctgatg gcaaaatggc tgaaaaagaa tgggtctacg attctcatag tcaagcctgg
     7141 tactacttca aatctggtgg ctacatggcg aaaaatgaga cagtagatgg ttatcagctt
     7201 ggaagcgatg gtaaatggct tggaggaaaa gctacaaata aaaatgctgc ttactatcaa
     7261 gtagtgcctg tcacagccaa tgtttatgat tcagatggtg aaaagctttc ctatatatcg
     7321 caaggtagtg tcgtatggct agataaggat agaaaaagtg atgacaagcg cttggctatt
     7381 actatttctg gtttgtcagg ctatatgaaa acagaagatt tacaagcgct agatgctagt
     7441 aaggacttta tcccttatta tgagagtgat ggccaccgtt tttatcacta tgtggctcag
     7501 aatgctagta tcccagtagc ttctcatctt tctgatatgg aagtaggcaa gaaatattat
     7561 tcggcagatg gcctgcattt tgatggtttt aagcttgaga atcccttcct tttcaaagat
     7621 ttaacagagg ctacaaacta cagtgctgaa gaattggata aggtatttag tttgctaaac
     7681 attaataata gccttttgga gaacaagggc gctactttta aggaagccga agaacattac
     7741 catatcaatg ccctttatct ccttgcccat agtgccctag aaagtaactg gggaagaagt
     7801 aaaattgcca aagataagaa taatttcttt ggcattacag cctatgatac gaccccttac
     7861 ctttctgcca agacatttga tgatgtggat aagggaattt taggtgcaac caagtggatt
     7921 aaggaaaatt atatcgatag gggaagaact ttccttggaa acaaggcttc tggtatgaat
     7981 gtggaatatg cttcagaccc ttattggggc gaaaaaattg ctagtgtgat gatgaaaatc
     8041 aatgagaagc taggtggcaa agattagtac tataagtgaa tatgatttga gtgaatagta
     8101 agttaaaaat cctgatttca agtaaaatca ggattttttc atggatgcaa tttttttgga
     8161 gtctggtgtg acgcggaggg tcttttgtcc tgtgtaagtg acaaagccgg gttttccacc
     8221 agttggttta ttgagttttt tgacttcaat catatctacc tgcaccagat tcgacaggcg
     8281 cccttgagag aagtaggcag ctaactctgc tgcgtctgtc ttgactgcat cagatgggtc
     8341 aagatttcct gagatgacaa catggcttcc aggaatgtcc ttagcatgga accaaagttc
     8401 ctccttgcgg gccattttaa aggtcaattc ctcattttga agattgtttc gtccgacata
     8461 gatgatggtt ttgccatcgc ttgctagata ttgttctagt tttttgcgtt tctggatttt
     8521 ctcccgttgt cttctgcgga taaaacctgt ttgaatcaat tcttcacgga tttcagcgat
     8581 ttcttccagt ccagcttggt tgaggacggt ttctacactt tccagataga gaatagtggc
     8641 tttggtttct tcaatcaaat cagtcaagta tttgacagct tctttgagtt tctgataccg
     8701 tttaaaatag cgttgggcat tctggttggg agtcagagcc ttatcaagcg caatcatgat
     8761 aggttggttg gtatagtagt tgtctaggat aacctggtct tggtcgttag gcacttggtg
     8821 gaggaaggtt gtcagcaatt ctcctttttg acgaaattct tcagcgttgt ctgtcgccag
     8881 taactctttt tcctgttttt tgagtttgtg tcggtttttc tgaagttcat tttcaacacg
     8941 acgaatcagt tcactggcct gctgtttgac gcggtcgcgc tcagccttat ccttatagta
     9001 ggtgtccaac aaatcagaaa gatttgcaaa aggctctccc acctgatttg caaaaggaac
     9061 tggactgaag gaagtctcag tcaagcatgg cttggtttcc tgattgaaaa aatttcggaa
     9121 agcggaaagt ttttcactaa ccagtatcct ttccaattca tttgccgtat cgcgtcccag
     9181 accttgaaag aggctttgaa gattttttgc tgttagttct tgggtttgca ggatttcaaa
     9241 gagcttttca tccttgatag taaaaggatt gagagattct gtacttggcg gagcgatata
     9301 ggtcgatcct ggaagtaagg tgcggtagct attttgtgaa aaaccgacgt gtttgataac
     9361 ttcgaggatt ttatgactgc ttttatcgac cagtagaata ttactgtgtt tccccataat
     9421 ttcgataatc aaggtagcct ggatatggtc tccaatctcg tttttattgg aaactgtcat
     9481 ttccacaata cggtcatttt ccacttgctc aatcgactca atcagggccc cctgcaaata
     9541 ctttctcaaa accatgataa aggtagaagg ttgggctgga ttttcaaaag tcgtttgggt
     9601 cagctgaatg cgtccaaaaa ctggatgggc agaaaggagc aggcgatggc tttggcgatt
     9661 gctgcggatt tgcaagacca actcttgttc aaaaggctga ttgattttct ggatgcgacc
     9721 attcactaat tcgcttcgca attcctcaac tatgtggtgt aaaaaaaatc cgtcaaatga
     9781 catcgttctc tccttgtgat tgtattccat agtattatat caaaaaggta gaataaaatc
     9841 atggaaatgt ggtataataa agccaagtaa agagaaacga gaagcacatg tatattgaaa
     9901 tggtagatga aactggtcaa gtttcaaaag aaatgttgca acaaacccaa gaaattttgg
     9961 aatttgcagc ccaaaaatta ggaaaagaag acaaggagat ggcagtcact tttgtgacca
    10021 atgagcgtag tcatgaactt aatctggagt accgtgacac cgaccgtccg acagatgtca
    10081 tcagccttga gtataaacca gaattggaaa ttgcctttga cgaagaggat ttgcttgaaa
    10141 atccagaatt ggcagagatg atgtctgagt ttgatgccta tattggggaa ttgttcatct
    10201 ctatcgataa ggctcatgag caggccgaag aatatggtca cagctttgag cgtgagatgg
    10261 gcttcttggc agtacacggc tttttacata ttaacggcta tgatcactac actccggaag
    10321 aagaagcgga gatgttcggt ttacaagaag aaattttgac agcctatgga ctcacaagac
    10381 aataaacgaa aatggaaaaa tcgtgacctg atatccagtt tagaatttgc catcacaggg
    10441 atttttactg ctatcaagga agaacgaaat atgcgaaaac atgcagtgac ggctctagtg
    10501 gttatccttg caggttttgt ttttcaggtg tcacgaatcg aatggctctt tctcctattg
    10561 agcattttct tggtagtagc ctttgagatt atcaactctg ctattgaaaa tatggtggat
    10621 ttggccagtc actatcactt ttccatgctg gctaaaaatg ccaaggatat ggcggctggc
    10681 gcggtattag tggtttctct tttcgcagcc ttaacaggtg cattgatttt tctcccacga
    10741 atctgggatt tattatttta aacagtaaga ggaaattatg acttttaaat caggctttgt
    10801 agccatttta ggacgtccca atgttgggaa gtcaaccttt ttaaatcacg ttatggggca
    10861 aaagattgcc atcatgagtg acaaggcgca gacaacgcgc aataaaatca tgggaattta
    10921 cacgactgat aaggagcaaa ttgtctttat cgacacacca gggattcaca aacctaaaac
    10981 agctctcgga gatttcatgg ttgagtctgc ctacagtacc cttcgcgaag tggacaccgt
    11041 tcttttcatg gtgcctgctg atgaagcgcg tggtaagggg gacgatatga ttatcgagcg
    11101 tctcaaggct gccaaggttc ctgtgatttt ggtggtgaat aaaatcgata aggtccatcc
    11161 agaccagctc ttgtctcaga ttgatgactt ccgtaatcaa atggacttta aggaaattgt
    11221 tccaatctca gcccttcagg gaaataacgt gtctcgtcta gtggatattt tgagtgaaaa
    11281 tctggatgaa ggtttccaat atttcccgtc tgatcaaatc acagaccatc cagaacgttt
    11341 cttggtttca gaaatggttc gcgagaaagt cttgcatcta actcgtgaag agattccgca
    11401 ttctgtagca gtagttgttg actctatgaa acgagacgaa gagacagaca aggttcacat
    11461 ccgtgcaacc atcatggttg agcgcgatag ccaaaaaggg attatcatcg gtaaaggtgg
    11521 cgctatgctt aagaaaatcg gtagcatggc ccgtcgtgat atcgaactca tgctaggaga
    11581 caaggtcttc ctagaaacct gggtcaaggt caagaaaaac tggcgcgata aaaagctaga
    11641 tttggctgac tttggctata atgaaagaga atactaagta gaggtaggct catgcctgct
    11701 tcttgttttt acagaaggag acttatgcct gaattacctg aggttgaaac cgtttgtcgt
    11761 ggcttagaaa aattgattat aggaaagaag atttcgagta tagaaattcg ctaccccaag
    11821 atgattaaga cggatttgga agagtttcaa agggaattgc ctagtcagat tatcgagtca
    11881 atgggacgtc gtggaaaata tttgcttttt tatctgacag acaaggtctt gatttcccat
    11941 ttgcggatgg agggcaagta tttttactat ccagaccaag gacctgaacg caagcatgcc
    12001 catgttttct ttcattttga agatggtggc acgcttgttt atgaggatgt tcgcaagttt
    12061 ggaaccatgg aactcttggt gcctgacctt ttagacgcct actttatttc taaaaaatta
    12121 ggtcctgaac caagcgaaca agattttgat ttacaggtct ttcaatctgc ccttgccaag
    12181 tccaaaaagc ctatcaaatc ccatctccta gaccagacct tggtagctgg acttggcaat
    12241 atctatgtgg atgaggttct ctggcgagct caggttcatc cagctagacc ttcccagact
    12301 ttgacagcag aagaagcgac tgccattcat gaccagacca ttgctgtttt gggccaggct
    12361 gttgaaaaag gtggctccac cattcggact tataccaatg cctttgggga agatggaagc
    12421 atgcaggact ttcatcaggt ctatgataag actggtcaag aatgtgtacg ctgtggtacc
    12481 atcattgaga aaattcaact aggcggacgt ggaacccact tttgtccaaa ctgtcaaagg
    12541 agggactgat gggaaaaatc atcggaatca ctgggggaat tgcctcaggt aagtcaactg
    12601 tgacaaattt tctaagacag caaggctttc aagcagtgga tgccgacgca gtcgttcacc
    12661 aactacagaa acctggtggt cgtctgtttg aggctatagt acagcacttt gggcaagaaa
    12721 tcattcttga aaacggagaa ctcaatcgcc ctctcctagc tagtctcatc ttttcaaatc
    12781 ctgaagagca aaaatggtct aatcaaattc aaggggagat tatccgtgag gaactggcta
    12841 ctttgagaga acaattggct cagacagaag agattttctt catggatatt cccctacttt
    12901 ttgagcagga ctacagcgat tggtttgctg agacttggtt ggtctatgtg gaccgagatg
    12961 cccaagtaga acgcttaatg aaaagggacc agttgtccaa agatgaagct gagtctcgtc
    13021 tggcagccca gtggccttta gaaaaaaaga aagatttggc cagccaggtt cttgataata
    13081 atggcaatca gaaccagctt cttaatcaag tgcatatcct tcttgaggga ggtaggcaag
    13141 atgacagaga ttaactggaa ggataatctg cgtattgcct ggtttggtaa ttttctgaca
    13201 ggagccagta tttctttggt tgtacctttt atgcccatct tcgtggaaaa tctaggtgta
    13261 gggagtcagc aagtcgcttt ttatgcaggc ttagcaattt ctgtctctgc tatttccgcg
    13321 gcgctctttt ctcctatttg gggtattctt gctgacaaat acggccgaaa acccatgatg
    13381 attcgggcag gtcttgctat gactatcact atgggaggct tggcctttgt cccaaatatc
    13441 tattggttaa tctttcttcg tttactaaac ggtgtatttg caggttttgt tcctaatgca
    13501 acggcactga tagccagtca ggttccaaag gagaaatcag gctctgcctt aggtactttg
    13561 tctacaggcg tagttgcagg tactctaact ggtcccttta ttggtggctt tatcgcagaa
    13621 ttatttggca ttcgtacagt tttcttactg gttggtagtt ttctattttt agctgctatt
    13681 ttgactattt gctttatcaa ggaagatttt caaccagtag ccaaggaaaa ggctattcca
    13741 acaaaggaat tgtttacttc agttaaatat ccctatcttt tgctcaatct ctttttaacc
    13801 agttttgtca tccaattttc agctcaatcg atcggcccta ttttggctct ttatgtacgc
    13861 gacttagggc agacagagaa tcttcttttt gtctctggtt tgattgtgtc cagtatgggc
    13921 ttttccagca tgatgagtgc aggagtcatg ggcaagctag gtgacaaggt gggcaatcat
    13981 cgtctcttgg ttgtcgccca gttttattca gtcatcatct atctcctctg tgccaatgcc
    14041 tctagccccc ttcaactagg actctatcgt ttcctctttg gattgggaac cggtgccttg
    14101 attcccgggg ttaatgccct actcagcaaa atgactccca aagccggcat ttcgaggatc
    14161 tttgccttca atcaggtatt cttttatctg ggaggtgttg ttggtcccat ggcaggttct
    14221 gcagtagcag gtcaatttgg ctaccatgct gtcttttatg cgacaagcct ttgtgttgcc
    14281 tttagttgtc tctttaacct gattcaattt cgaacattat taaaagtaaa ggaaatctag
    14341 tgcgagtaaa aattaatctc aaatgctcct cttgtgacag tatcaattac ctaaccagta
    14401 aaaattcaaa aacccatcca gacaagattg aggttttaaa gtattgtccc aaggaaagaa
    14461 aagtaaccct acatcttgaa tctaagtaga ttttatggta aaataatagg gattttaagg
    14521 agtttgatat gtataaccta ttattaacca ttttattagt attatctgtt gtgattgtga
    14581 ttgcaatttt catgcaacca accaaaaacc aatccagcaa tgtatttgat gccagttcag
    14641 gtgatttgtt tgaacgcagt aaagctcgcg gttttgaagc tgtaatgcag cgtttgacag
    14701 ggattttagt ctttttctgg ctagccattg ccttagcatt gacggtatta tcaagtagat
    14761 aagaaaataa tgggcaggac taggtctttg cctcttttta tttttaaagg atgtttgaga
    14821 aggttttaca gtaaaagaaa attaaaaaat ctagaaagaa aatatgaaag atagaataaa
    14881 agaatattta caagacaagg gaaaggtgac tgttaatgat ttggctcagg ctttgggaaa
    14941 agacagttcc aaggattttc gtgagttgat taaaaccttg tccttaatgg aaagaaagca
    15001 ccagattcgc tttgaagaag atggcagtct gaccttggaa gccaagaaaa aacatgagat
    15061 taccctcaag gggatttttc atgcccataa aaatggtttt ggttttgtca gcctagaagg
    15121 ggaagaggac gacctttttg tagggaaaaa tgatgtcaac tatgctattg atggtgatac
    15181 cgtcgaggta gtgattaaga aagtcgctga ccgcaataag ggaacagcag cagaagccaa
    15241 aattattgat atcctagaac acagtttgac aacagttgtc gggcaaatcg ttctggatca
    15301 ggaaaagcct aagtatgctg gctatattcg ttcaaaaaat cagaaaatca gtcaaccgat
    15361 ttatgttaag aaaccagccc taaagttaga aggaacagaa gttctcaagg tctttatcga
    15421 taaataccca agcaagaaac atgatttctt tgtcgcgagt gttctcgatg tagtgggaca
    15481 ctcaacggat gtcggaattg atgttcttga ggtcttggaa tcaatggaca ttgtatccga
    15541 gtttccagaa gctgttgtta aggaagcaga aagtgtgcct gatgctccgt ctcaaaagga
    15601 tatggaaggt cgtctggatc taagagatga aattaccttt accattgacg gtgcggatgc
    15661 caaggacttg gacgatgcag tgcatatcaa ggctctgaaa aatggcaatc tggagcttgg
    15721 ggttcacatc gcagatgttt cttattatgt gaccgagggg tctgcccttg acaaggaagc
    15781 ccttaaccgt gcgacttctg tttacgtgac agaccgagtg gtgccaatgc ttccagaacg
    15841 actatcaaat ggcatctgct ctctcaatcc ccaagttgac cgcctgaccc agtctgctat
    15901 tatggagatt gataaacatg gtcgtgtggt caactatacc attacacaaa cagttatcaa
    15961 gaccagtttt cgtatgacct atagcgatgt caatgatatc ctagctggcg atgaagaaaa
    16021 gagaaaagaa taccataaaa ttgtaccaag tatcgaactc atggccaagc ttcatgaaac
    16081 tttagaaaac atgcgtgtga aacgtggagc tctcaatttt gataccaatg aagcgaagat
    16141 tttagtggat aaacaaggta agcctgttga tatcgttctt cggcagcgtg gcattgccga
    16201 gcggatgatt gagtctttta tgttgatggc taatgaaaca gttgccgaac atttcagcaa
    16261 gttggatttg ccttttatct atcgaattca cgaggagcct aaggctgaaa aggttcagaa
    16321 gtttattgat tatgcttcga gttttggctt gcgcatttat gggactgcca gtgagattag
    16381 ccaggaggca cttcaagaca tcatgcgtgc tgttgaggga gaaccttatg cagatgtatt
    16441 gtccatgatg cttcttcgct ctatgcagca ggctcgttat tcggagcaca atcacggcca
    16501 ctatggacta gctgctgact attatactca ctttaccagt ccaattcgtc gttatccaga
    16561 ccttcttgtt caccgtatga ttcgggatta cggccgttct aaggaaatag cagagcattt
    16621 tgaacaagtg attccagaga ttgcgaccca gtcttccaac cgtgaacgtc gtgccataga
    16681 agctgagcgt gaagtcgaag ccatgaaaaa ggctgagtat atggaagaat acgtgggtga
    16741 agagtatgat gcagttgtat caagtattgt caaattcggt ctctttgtcg aattgccaaa
    16801 cacagttgaa ggcttgattc acatcactaa tctacctgaa ttttatcatt tcaatgagcg
    16861 tgatttgact cttcgtggag aaaaatcagg tatcactttc cgagtgggtc agcagatccg
    16921 tatccgtgtt gaaagagcgg ataaaatgac tggagagatt gatttttcat tcgtacctag
    16981 tgagtttgat gtgattgaaa aaggcttgaa acagtctagt cgtagtggca gagggcgtgg
    17041 ttcaaatcgt cgttcggata agaaggaaga caagagaaaa tcaggacgct caaatgataa
    17101 gcgtaagcat tcacaaaaag acaagaagaa aaaaggaaag aaaccttttt acaaggaagt
    17161 agctaagaaa ggagccaagc atggcaaagg gcgagggaaa ggtcgtcgca caaaataaaa
    17221 aggcacgcca cgactataca atcgtagata cgctagaggc agggatggtc ctgactggaa
    17281 ctgaaatcaa gagtgtacga gctgctcgaa ttaatctcaa ggatggcttt gctcaagtga
    17341 aaaatggaga agtttggctg agtaatgttc atatcgcgcc ttacgaagag ggcaatatct
    17401 ggaaccagga accagaacgt cgtcgtaaac tcctgctcca taaaaagcaa attcaaaaat
    17461 tggaacaaga gaccaaaggg acaggaatga ccttagttcc ccttaaggtc tatataaaag
    17521 atggctacgc taagcttctt ttaggacttg ccaaagggaa gcatgactat gacaaacggg
    17581 agtctatcaa acgtcgtgag caaaatcgag atatcgcgcg tgtgatgaaa gctgttaatc
    17641 agcgataaaa agaggaattg aaaatggaaa aattagttgc ctataaacgc atgcctttgt
    17701 ggaataaaca aacaatgcct gaagctgttc agcaaaagca caatacaaaa gttgggactt
    17761 gggggaaaat tactgtcttg aagggagctc tcaagtttat tgaattgaca gaagaagggg
    17821 aagttctagc tgaacacctc tttgaagcag gggcagacaa tccaatggcc caacctcaag
    17881 cctggcaccg agtggaagct gccacagatg atgtggaatg gtacttggaa ttttattgta
    17941 aacctgagga ttattttgct aaaaaataca ataccaatcc tgttcattca gaggtcctag
    18001 aggccatgca gacagtgaaa caagggaaag ctttggattt gggttgtggt caggggcgta
    18061 attctctttt tctagcccag caagattttg atgtgacggc tgtagatcaa aatggactag
    18121 ctcttgaaat cttgcaaagc attgtggagc aggaagattt ggacatgcct gttggccttt
    18181 acgatatcaa ttcagctagc attgaacaag aatatgattt tatcgtttca acagttgttc
    18241 tcatgtttct acaagcggac cgcattccag ctattattca aaatatgcag gagaaaacca
    18301 gtgttggtgg ttacaacctt atcgtttgtg ccatggacac ggaggattat ccttgctcgg
    18361 ttaacttccc attcaccttt aaagaaggag aactggcaga ctattacaag gattgggaat
    18421 tggttaagta caatgaaaat ccaggccatt tgcaccgtcg cgatgagaat ggcaatcgta
    18481 ttcaactacg ctttgcgacc ttactagcta agaaaatcaa gtaaacacac atgaagatta
    18541 ggaattttcc tgatcttttt tcttttttac gaatgatata gaaaaggagg gaattcatgt
    18601 ttgttgcgag agatgctagg ggagaattgg taaatgtgtt agaggataaa cttgagaagc
    18661 aagcatacac ctgcccagct tgtggaggcc agctccattt gcgtcaagga ccaagtgtac
    18721 ggacgcattt tgcccataaa tctttaaaag actgtgattt tttctttgaa aatgaaagtc
    18781 cagaacacct ggccaataag gaatccctct atcactggtt gaaaaaagag acaaaggttc
    18841 aattagagta cccgctttca gaacttaaac agattgcgga tgtatttgta aatggcaatc
    18901 tagctctaga agttcagtgt agtcccttgc ctcagaaagt ccttaaagag cgaagtgagg
    18961 gctatcgtag tcagggttac caagtactgt ggttgctggg tcaaaaactg tggctcaagg
    19021 agcgtttgac tcgtctacag caaggttttc tttatttcag tcaaaacatg ggcttttatg
    19081 tttgggaatt agacaaggga aaacaagttt taagactcaa atacctgatt taccaggatc
    19141 tccgcggtaa actccattat caaatcaagg aattttccta tggccaaggt agtttattgg
    19201 aaatattgcg tcttccctat aagaaacaaa aaatatctca ttttacagtt tctgaggaca
    19261 aggacatctg tcgctatatc cggcaacaac tttattatca aaatctcttt tggatgaaag
    19321 aacaagcaga agcctatcaa aagggagaaa atatcctgac ttatggactg aaagaatggt
    19381 atccacaaat tcgaccaata gtgggcaaat ttttccagat tgaacaagac ttgactagct
    19441 attatcagca cttttatacc tattaccaaa aaaatcctca aaatgattgg caaaagcttt
    19501 atccaccagc cttttatcag caatatttct tgaaaaatat ggtagaatag aaaggatgga
    19561 ggaatctaat ggtattacaa agaaatgaaa taaatgaaaa agatacatgg gatctatcaa
    19621 cgatctaccc aactgaccag gcttgggaag aagccttaaa agatttaaca gaacaattgg
    19681 agacagtagc ccagtatgaa ggccatctct tggatagtgc ggataaccta ctagaaatca
    19741 ctgaattttc tcttgaaatg gaacgccaga tagagaagct ttacgtttat gctcatatga
    19801 agaatgacca ggatacacgt gaagctaagt atcaagagta ctatgccaag gccatgacac
    19861 tctacagcca gttagaccaa gccttttcat tctatgagcc tgaatttatg gagattagcg
    19921 aaaagcagta tgctgacttt ttagaagctc aaccaaagct gcaggtttat caacactatt
    19981 ttgacaagct cttgcaaggc aaggatcacg ttctttcaca acgtgaagaa gaattattgg
    20041 ctggagctgg agaaatcttt ggttcagcaa gtgaaacctt cgctatcttg gacaatgcgg
    20101 atattgtgtt cccttatgtc ctagacgatg atggtaaaga agttcagcta tctcatggga
    20161 cttacacacg tttgatggag tctaaaaaac gtgaggttcg ccgtggtgcc tatcaagctc
    20221 tttatgcgac ttacgaacaa ttccaacaca cctatgccaa aaccttgcaa accaatgtta
    20281 aggtgcaaaa ttaccgtgct aaagttcgta actacaagag tgctcgtcat gcagccctcg
    20341 cagcgaattt tgttccagaa agtgtttatg acaatttggt agcagcagtt cgcaagcatt
    20401 tgccactctt acatcgctat cttgagcttc gttcaaaaat cttggggatt tcagatctca
    20461 agatgtacga tgtctacaca ccgctttcat ctgttgaata caattttacc taccaagaag
    20521 ccttgaaaaa agcagaagat gctttggcag tcttgggtga ggattacttg agccgtgtca
    20581 aacgtgcctt cagcgagcgt tggattgatg tttacgaaaa tcaaggcaag cgttcaggtg
    20641 cctactctgg tggttcttac gataccaatg cctttatgct tctcaactgg caggacaatc
    20701 tggacaatct ctttactctt gttcatgaaa caggtcacag tatgcattca agctatactc
    20761 gtgaaactca gccttatgtt tacggagatt actctatctt tttggctgag attgcctcaa
    20821 ctaccaatga aaatatcttg acggagaaat tattggaaga agtggaagac gacgcaacac
    20881 gctttgctat tctcaataac ttcctagatg gtttccgtgg aacagttttc cgccaaactc
    20941 aatttgctga gtttgaacac gccattcacc aagcagatca aaatggggag gtcttgacaa
    21001 gcgatttcct aaataaactc tacgcagact tgaaccaaga gtattatggt ttgagtaagg
    21061 aagacaatcc tgaaatccaa tacgagtggg ctcgcattcc acacttctac tataactact
    21121 atgtatatca atattcaact ggctttgcgg ccgcctcagc cttggctgaa aaaattgtcc
    21181 atggtagtca agaagaccgt gaccgctata tcgactacct caaggcaggt aagtcggact
    21241 atccacttaa tgtcatgaga aaagctggtg ttgatatgga gaaggaagac tacctcaacg
    21301 atgcctttgc agtctttgaa cgccgtttaa atgagtttga agcccttgtt gaaaaattag
    21361 gattggcata aaatggttga atcgtatagt aagaatgcta accataacat gcgtcgtcct
    21421 gtcgtcaaag aagaaattgt agacttgatg cgtcagcgtc aaaagcaggt cacaggtttc
    21481 ttgaaagaat tggaagactt tgcccgcaag gaaaatattc ctattattcc ccatgaaacg
    21541 gttgcttatt tccgttttct tatggaaacc atgcagccta aaaatattct ggaaattggg
    21601 acggctatcg gtttttcagc tctcttgatg gctgaacatg cgccaaatgc taagattaca
    21661 actattgatc gtaatccaga aatgattggt tttgccaagg aaaattttgc ccagtttgac
    21721 agtcgcaagc aaatcactct cctagaggga gatgcggttg atgtcttatc tacactgaca
    21781 gagtcttatg atttcgtctt tatggattct gccaagtcta aatacatcgt ctttctgcca
    21841 gaaatcctca aacatttgga agttggtggt gtggttgtct tggatgatat ttttcaaggt
    21901 ggtgatgttg ccaaggatat tatggaagtc cgtcgtggtc agcgaaccat ttatcgaggc
    21961 cttcaaaaat tatttgatgc aaccttagac aatccagaac tcaccgcaac attagtgcct
    22021 ttaggagatg gtattctcat gcttcgtaaa aatgtagcag atgttcaact gtctgaaagc
    22081 gaatgatttt cagaaaaatt taagaaaaaa tagtaaaata gatagagtaa cacttatctc
    22141 aaaggagtag acatgaagaa aaaattattg gcaggtgcca tcacactatt atcagtagca
    22201 actttagcag cttgttcgaa agggtcagaa ggggcagacc ttatcagcat gaaaggggat
    22261 gtcatcacag aacatcaatt ttatgagcaa gtgaaaaaca acccttcagc ccaacaagtc
    22321 ttgttaaata tgaccatcca aaaagttttt gaaaaacaat atggctcaga gcttgatgat
    22381 aaagaggttg atgatactat tgccgaagaa aaaaaacaat atggcgaaaa ctaccaacgt
    22441 gtcttgtcac aagcaggtat gactcttgaa acacgtaaag ctcaaattcg tacaagtaaa
    22501 ttagttgagt tggcagttaa gaaggtagca gaagctgaat tgacagatga agcctataag
    22561 aaagcctttg atgagtacac tccagatgta acggctcaaa tcatccgtct taataatgaa
    22621 gataaggcca aagaagttct cgaaaaagcc aaggcagaag gtgctgattt tgctcaatta
    22681 gccaaagata attcaactga tgaaaaaaca aaagaaaatg gtggagaaat tacctttgat
    22741 tctgcttcaa cagaagtacc tgagcaagtc aaaaaagccg ctttcgcttt agatgtggat
    22801 ggtgtttctg atgtgattac agcaactggc acacaagcct acagtagcca atattacatt
    22861 gtaaaactca ctaagaaaac agaaaaatca tctaatattg atgactacaa agaaaaatta
    22921 aaaactgtta tcttgactca aaaacaaaat gattcaacat ttgttcaaag cattatcgga
    22981 aaagaattgc aagcagccaa tatcaaggtt aaggaccaag ccttccaaaa tatctttacc
    23041 caatatatcg gtggtggaga ttcaagctca agcagtagta catcaaacga atagtccaaa
    23101 tcaatgagtc agggaaaaaa actcgacttc aggaaaaaat gaagcaaaca ttcccacaat
    23161 aaaacgcata gtacaaggtt tgtactgccc ccaaaagtta gacaattaat ttatctgaag
    23221 gatttagttc tgta
//
LOCUS       DAVMGZ010000018        21826 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000018 DAVMGZ010000000
VERSION     DAVMGZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.019"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(134..475)
                     /locus_tag="VYQ93_000589"
     CDS             complement(134..475)
                     /locus_tag="VYQ93_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV3335327.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            599..1450
                     /gene="rgg"
                     /locus_tag="VYQ93_000590"
     CDS             599..1450
                     /gene="rgg"
                     /locus_tag="VYQ93_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV3335328.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRKTPDS"
     gene            1641..2888
                     /locus_tag="VYQ93_000591"
     CDS             1641..2888
                     /locus_tag="VYQ93_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV3335329.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSAFILFLGKSKLSLSISSADGEKLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQVCVLLAIIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGFSLLAIFLTILNLKNDI"
     gene            2966..4255
                     /locus_tag="VYQ93_000592"
     CDS             2966..4255
                     /locus_tag="VYQ93_000592"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV3335330.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRAYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTIGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     RQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            4246..4326
                     /locus_tag="VYQ93_000593"
                     /pseudo
     CDS             4246..4326
                     /locus_tag="VYQ93_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            4398..4745
                     /locus_tag="VYQ93_000594"
     CDS             4398..4745
                     /locus_tag="VYQ93_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335331.1"
                     /translation="MIDKVVRNLLLTFFLCKMTKIINFLTTILVKKKKMCYNVSKLRE
                     KKKGNAEMSRLLQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDKKGKEKFKLQ
                     RIEDIQTVEFDGE"
     gene            4755..4961
                     /locus_tag="VYQ93_000595"
     CDS             4755..4961
                     /locus_tag="VYQ93_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3335332.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            4961..5158
                     /locus_tag="VYQ93_000596"
     CDS             4961..5158
                     /locus_tag="VYQ93_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335333.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            5161..5895
                     /locus_tag="VYQ93_000597"
     CDS             5161..5895
                     /locus_tag="VYQ93_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV3335334.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            5907..6548
                     /locus_tag="VYQ93_000598"
     CDS             5907..6548
                     /locus_tag="VYQ93_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3335335.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            6566..6796
                     /locus_tag="VYQ93_000599"
     CDS             6566..6796
                     /locus_tag="VYQ93_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335336.1"
                     /translation="MKRVILVAVIQAVILFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            6832..6912
                     /locus_tag="VYQ93_000600"
                     /pseudo
     CDS             6832..6912
                     /locus_tag="VYQ93_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7193..8956
                     /gene="aspS"
                     /locus_tag="VYQ93_000601"
     CDS             7193..8956
                     /gene="aspS"
                     /locus_tag="VYQ93_000601"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV3335337.1"
                     /translation="MKRSMYAGRVREEHIGQEIALKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALVLEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            8934..9875
                     /locus_tag="VYQ93_000602"
     CDS             8934..9875
                     /locus_tag="VYQ93_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV3335338.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINALFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(10040..10120)
                     /locus_tag="VYQ93_000603"
                     /pseudo
     CDS             complement(10040..10120)
                     /locus_tag="VYQ93_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(10256..11242)
                     /locus_tag="VYQ93_000604"
     CDS             complement(10256..11242)
                     /locus_tag="VYQ93_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3335339.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(11252..12052)
                     /locus_tag="VYQ93_000605"
     CDS             complement(11252..12052)
                     /locus_tag="VYQ93_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV3335340.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(12305..13147)
                     /locus_tag="VYQ93_000606"
     CDS             complement(12305..13147)
                     /locus_tag="VYQ93_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3335341.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(13149..14441)
                     /locus_tag="VYQ93_000607"
     CDS             complement(13149..14441)
                     /locus_tag="VYQ93_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3335342.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(14552..15823)
                     /locus_tag="VYQ93_000608"
     CDS             complement(14552..15823)
                     /locus_tag="VYQ93_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV3335343.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            16373..17890
                     /gene="malQ"
                     /locus_tag="VYQ93_000609"
     CDS             16373..17890
                     /gene="malQ"
                     /locus_tag="VYQ93_000609"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV3335344.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLGASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            17916..20174
                     /gene="glgP"
                     /locus_tag="VYQ93_000610"
     CDS             17916..20174
                     /gene="glgP"
                     /locus_tag="VYQ93_000610"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV3335345.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(21219..21791)
                     /locus_tag="VYQ93_000611"
     CDS             complement(21219..21791)
                     /locus_tag="VYQ93_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335346.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
BASE COUNT         6398 a         4101 c         4652 g         6675 t
ORIGIN      
        1 tatccataac ttcaaaacag tgttttgagc aacctgcggc tagttttcta gtttgccctt
       61 tgattttcat tgagtatcaa aaagcaagcc atttttggct tgcttttttc atctttaaaa
      121 atgattggcc tgcttaacga ggtggtagtc caagggcaat acggccatag cgactgattc
      181 gtgtgatttt ccaagcaggc gaccaggtaa cttcaacctt gatatctttg ataccctcga
      241 tttgtttcag acctgccacg atttcaatag gcaggctttc ggcgcaatca caggcagtat
      301 cggtgaaggt catgacaatc ttgcagagcc ccgtttcatc cagattgatt tcataaatca
      361 gacctagatt gtaaacatcc aattccacat ctgtatcaaa aactttctct agtttttcga
      421 tgatttggtc ttgcaaggcc aaagcacggt cattgatttt gatatcgtct ctcatagcag
      481 tccctccacg tgataaatat cctctatttt ctcataattc tgacaatttg gcaagttttt
      541 gatgaatttt ctgaaaaata tgataaaatg aagaaaatac ggaatcaagg ggaagtctat
      601 ggagcagatt ggaaaagtct ttagacaatt acgagagtca agaaatatct cactgagaca
      661 agcaaccgga ggacaatttt cgccgtctat gttgtcccgt tttgaaacag gtcagagtga
      721 gctttcggtg gaaaagtttc tatttgctct ggaaaatata tctgccagtg tggaggagat
      781 cctctttctg gcgagaggtt tccagtatga tacagattct gagttgagaa aagaaattac
      841 agatgtcttg gaaccaaaga atatagcacc tcttgaggac ttatatcgca gggagtatca
      901 aaagcatgcc cattctcaca acaaacagaa acatattcta aatgccatta ttatcaagtc
      961 ttatatgaag agcatagatg aaagggtaga cttaacagca gaggaaggaa aagtccttca
     1021 tgattatttg ttttctaccg agatctgggg aatctatgaa ctcaatttat tttcagtcag
     1081 ttctccattt ttatctgttt ctctttttac tagatatgta cgagaaatgg tacgtaaatc
     1141 tgattttcta atggaaatgt ctggcaatcg aaaccttttt catactatac tattgaatgg
     1201 ctttttagct agtattgagt gtgaagaatt taccaatgcc tattatttta aacgtgttat
     1261 cgaagagcat ttctacaagg aaaatgagac ctatttccga attgtttatc tgtgggcaga
     1321 aggtctcctt gatagcaagc aaggtagagt caaggaaggc cagaaaaaga tggaggatgc
     1381 tgtccgtatt tttgagatgc ttggctgtaa taaatctgcc gaatactata gaaaaactcc
     1441 cgattcttga atatctgcaa accaagaaaa aatttgaagc gatagaactg ttgccctctc
     1501 tcaggtcatt acaaaagttc tatcgttttt ttgctcattt tgtttgttgc atatattcaa
     1561 aagaatcgtt tcgcaaattc ataagtgcta tactaaagct attcttgatg tagaacttag
     1621 aacaaggagg gagattatct atgaaactat tgtttagaaa tccagcttat agaattttga
     1681 ctttatcacg cttcttcaat gcttttggtg cttcgatttt caacctggtc tttatcgtct
     1741 atgcatcgac cttgtcacaa gcatcttttg ctgttgctat ggcgaatatt gtcatgattc
     1801 ttccgaccct ctttacagtt tttgtaggga ttcgggcaga ttacacgagg gacaaggtca
     1861 aatggatggt ctatagcggt ttgtttcagg cggttctatt ttttctagca gccctagttg
     1921 ttcagcaagc tagtctcttt gccttttcta gcctgtgttt gatcaatgtc attagtgata
     1981 tcatcagtga ttttgcaggt ggcctgcgta tgcctcttat taaggaaaaa gtagctgaag
     2041 atgatctgat ggaggcttat tctttttcac agttcatcac ctatatttca gctattggtg
     2101 gtcaagcttt cggagtctgg ctcttagctc tatcggtcaa caatttttcc ctcgttgcgg
     2161 gaattaatgc ttgctttttc ctcgtatcag cctttattct ttttttagga aaaagcaaat
     2221 tgagcctgtc aatttcatct gctgatggtg aaaaactaaa aaatgagaag ctttctatca
     2281 aagaccagtt cctaacaatt taccgaaatt tacgtctcgt ttttcttaaa agtggacaga
     2341 aaaactttgg ttttatgctc tttgctgtct tgcttattaa ttccttgggt ggcgctttgg
     2401 gtggaattta taatatcttc tttttgagcc attctctctt gaatttttct tatacagagg
     2461 cactatttat caatcaagtc tgtgttttgt tagcaatcat cattagtagc cttacgggca
     2521 atgattattt tgggaagcag tccttgccta gattgatgat gtgggagacc gtaggactta
     2581 gtctagttgg tttggctaat ttattcaatc aagtcgtgct tggtttgcta tttctcttct
     2641 ttactctgta tgtgtctggt aaagttcaac caaagattag tgccatgctc atgaaaaatc
     2701 tagctccaga ggttctagct cgtactagta attttttagg tctattgttt accttatcca
     2761 tacctgtggg aacagcttgt ttttcactag tagccgtatg gaatatacag ttgacttgga
     2821 tgctatttgt tggtttttcc ttgctagcta tttttttgac aattcttaat ctcaaaaatg
     2881 atatctaaat cctaattttt ttcttactgt tttaatccct ttatttatgg taaaataaga
     2941 ctattaagtt taaagaggat tccctatgaa attacaaaaa ccaaaaggaa cgcaggatat
     3001 tttacctgct gaatctgcca agtggcagta cgttgagggc tttgcccgtg agattttcaa
     3061 gcgctataac tatgcagaag tgcgcacgcc tatttttgag cattacgagg ttatcagtcg
     3121 ctctgtcgga gatacaacgg atatcgtaac caaggaaatg tacgattttt atgacaaggg
     3181 tgaccgccat attaccctcc gtccagaagg aactgcgccc gttgtccgtg cctatgtgga
     3241 aaataaactc ttcgccccag aagtgcaaaa gccaagcaag ttctactata tgggacctat
     3301 gttccgttat gagcgtccac aggcagggcg cttgcgccaa ttccaccaga ttggtgttga
     3361 gtgttttggc tctagcaatc cagctaccga tgtggaaaca atcgttatgg cagcccattt
     3421 tttgaaggaa atcggtattc aaggtgtcaa attgcacctc aacactattg gaaatcctga
     3481 gagccgtgca gcctaccgcc aagccttgat tgactatttg acaccgctca aggagacctt
     3541 gtctaaggat agccaacgtc gcttggagga aaatcctctt cgtgtcttgg actctaagga
     3601 aaaagaagac aaggtggcag tagagaatgc gccgtctatc ttggactttc ttgatgaaga
     3661 aagccaagct cattttgatg ctgtgcgtca gatgttggaa aatcttggag tagattacat
     3721 catcgatacc aatatggtgc gtggtctgga ctactacaac cacaccattt tcgagtttat
     3781 cacagagatt gagggcaatg acttgacagt ctgtgcgggt ggtcgctacg atggtttggt
     3841 tgcttacttt ggaggccctg aaactgctgg atttggtttt gggcttggtg tagagcgcct
     3901 gcttctcatc cttgaaaaac aaggcgtggc cctccctatc gaaaatgccc tagatgtcta
     3961 tatcgcagtc ttgggtgatg gagcaaatgt caaagcccta gaactagtcc aagctcttcg
     4021 ccaacaaggt ttcaaagcag agcgtgatta cctcaaccgt aagctcaaag ctcagttcaa
     4081 gtcagccgat gtctttgcgg ctaagaccct catcacccta ggagagagcg aagtcgaaag
     4141 cagacaagtg acagtcaaga acaaccaaac ccgagaagaa gtgcaagtgt cacttgagac
     4201 aatcagccaa aacttctcag aaatctttga aaaattagga ttttatactc aatgaaaatc
     4261 aaagagcaaa ctaggaagct agccgcaggt tgctcaaaac actgttttga ggttgtagat
     4321 gaaactgacg aagtcagtaa ccatacctac ggcaaggtga agctgacgtg gtttgaagag
     4381 attttagaag agtattaatg atagataaag tggtcaggaa cttactcctg acctttttcc
     4441 tttgcaaaat gacaaaaatc ataaactttt tgacaactat acttgtcaaa aagaaaaaga
     4501 tgtgttacaa tgtaagcaag ttaagagaaa agaagaaagg aaacgcagaa atgtcgagat
     4561 tattacaaga aatgattgga aaggaaccaa ttataacggg agtgtatatt gggccagata
     4621 actgggaagt tgtggatgtt gacgaggaat gggtcaaact acgtagtgtt gataaaaagg
     4681 ggaaagaaaa attcaagttg caacgtattg aagatatcca aaccgttgaa tttgacggag
     4741 agtaggtgtg taacatgcaa ctaaaaaatc gtctaaaaga gcttcgagct cgcgatggtc
     4801 tcaatcaaac cgacctagcc aaactggcag gggtttccag acagaccatt agcctactag
     4861 aacgggatga gtacacccca tccattatca ttgccctgaa aatatctcaa attttcaacg
     4921 aaacagtcga atcggtattt cgtttggagg aggacgagtg atgaacaagt ataaagtgat
     4981 ttattatgta gtggtcatag ctttattagt cagcgtattt ctactgattg ggatggacct
     5041 aagctggttt aatccctatc aaagcgacca atttgtttgg gtctactttg ctctcatccc
     5101 agtaactgaa tggattgaaa agaaatccaa aaatctagca agtgaaaaag gagaatgaat
     5161 atgaaaaaga aacgtagatt gttatttttg atgtctattg tcttgggggg gttcttgggc
     5221 atgtttgtag ggatgtttaa ggcacgtgtc gaatcccacg aaattatttt agatgtaaaa
     5281 gccttgatgc catggatatc agctatttgt ttactgatag gtttcattag tatgtttttg
     5341 actttcaatt tcttaaagaa aagcagaaaa tttcattcct tgtatcaaga ggaaatggat
     5401 gacgatctga atgaaaccta ttatgtgcaa atgtatcgga atcttgagtt tggaaccatt
     5461 gcttttaata ttacaggtgt agcgattcca ttggctattt ttatttcatt aagtgaggtg
     5521 attatattgc atacaaaccc tcaaacattt ttcctttctt tcttactctt tgtggtattc
     5581 ttagtcgctc aaaaatctct ttttaaaacc attgcgattg ttcgtcagtt tgatttggaa
     5641 tttttcgcta caccaaagga tgtcttgaac tatataaatt cttatgatga aggggagcgt
     5701 caggctaatt tggaacagag ttttcgaatt ttattccaat tacaccaata tgtcttacca
     5761 gccttatata tttttcttat tatcatttct ttcttgacag gagagattca gttactagct
     5821 ttcttgcttg taggagccat ccatgtttat atcaatgtga tgcagttacc tatggtaaaa
     5881 cgttatttca aataaaggag ttatctatga aattacttaa aaaccttggc tggattcttc
     5941 tagcccttct atccttttta tttatctatg gctttattca ggggctcgcg actgcgtcgc
     6001 ttgctttagg cgcttcaccc tatgctgtta ctctactcta tgtggccttg gctggagtat
     6061 atgtgtacgg catttacaaa tggtatcaga aggctcctgt ttatattgag aagagcggct
     6121 ttaaccgatt tatttggctt cctgccttgg tttggttctt atctctggtc gttcaattct
     6181 tcttgccaga tgatccttca gtaaatcaac aaatagcgac agacttgacc ttgtctcaac
     6241 cacttttctc attctttgct gtggttattt ttgctccttt gacggaagag attgttttta
     6301 gagggatgtt agcacgctat ctctttccta agcaggacaa tagtaaacga accctgattt
     6361 ttcttctggt atctagtctt ctatttgcct tgattcattt tccaggtgat gtgcaacaat
     6421 tttttgtcta ttttagcctt ggttttagtt tgggtttggc ttacattagc agaaaaggtc
     6481 tggtctacag tatttctctt cacgctttga ataatttagt cggctttttg atgattctca
     6541 tgctataata gagtcaggag gtcacatgaa acgagtaatt ttagtagcag tgattcaggc
     6601 agtcattcta tttttcatca ttggagcgct agcttatgcc ttcaaaggcg atttcttcta
     6661 taactatcta gcagttgtct ttgctcctat tgcaggtgta ctgcgttttg ggacggctta
     6721 cataacggaa attgtcttgc ctcgaaaggc agccgaaatc gctgaaaagc gtaaagcagg
     6781 caaaaattca aaataaaaga gtccggtgaa gttcatcgga tttttgtatt atactcaatg
     6841 aaaatcaaag agcaaactag gaagctagcc gcaggttgct caaaacactg ttttgaggtt
     6901 gtggatgaaa ctgacgaagt cagctcaaaa catggttttg aggttgtaga tgaaactgac
     6961 gaagtcagct caaaacatgg ttttgaggtt gtagatgaaa ctgacgaagt cagtaaccat
     7021 acctacggta aggcgacgct gacgtggttt gaagagattt tcgaagagta ttagcgctaa
     7081 tttttagggc tttcatctga cttttaaaga ctggcaaact tttctgatga ttttcatgaa
     7141 gagcctgcgc ttttatggta aaatagtaac agaataaaag aggagagaaa taatgaaacg
     7201 tagtatgtat gctggtcgtg ttcgtgagga acacatcgga caagaaatag ccttgaaagg
     7261 atgggttggc cgtcgtcgtg accttggtgg tttgatcttt atcgatcttc gtgaccgtga
     7321 aggaatcatg cagttggtta tcaaccctga aaaagtatct gcagaggtta tggcaacagc
     7381 tgaaagcctt cgtagcgaat ttgttattga ggtgactggt caggtcgctg cgcgtgagca
     7441 agccaatgat aagttgccaa ctggtgcggt tgagttaaac gtgacagctc ttattgtgct
     7501 taacacagct aagacaacac catttgagat taaggatggc attgaggcaa atgacgatac
     7561 acgtttgcgt taccgttacc ttgaccttcg tcgtccagaa atgttggaaa atcttaaact
     7621 tcgtgccaag gtgacccact ctatccgcaa ctatttggat gagttggagt ttatcgacgt
     7681 ggagacacca ttcctttcta agtcaacgcc tgaaggggcg cgtgattatt tagtgccgtc
     7741 tcgtgttaat aaggggcatt tttacgctct tcctcaaagt ccacaaatca cgaaacagct
     7801 cttgatgaat gctggttttg accgttacta ccaaatcgtt aaatgtttcc gtgacgagga
     7861 cttgcgtgga gaccgccagc ctgaatttac tcaggtcgac ttggaaacgt ccttccttac
     7921 tgagaaagaa atccaagata ttacagaagg cttgatcgcg cgcgtgatga aggaaacaaa
     7981 aggcatcgaa gtaacgctac cgttccctcg tatgaaatac gatgatgcta tggctcttta
     8041 cggttctgac aagccagata ctcgttttga catgttgctt caggacttga cagaagtggt
     8101 caaaggtgta gactttaaag tcttttcaga agcacctgct gtaaaagcga ttgtggtcaa
     8161 aggagctgcg gacaactatt cacgtaaaga catcgacaag atgacggaag tagccaaaca
     8221 gtatggtgcc aaaggtcttg cttgggtcaa ggtggttgat ggagaattaa acggaccagt
     8281 tgccaagttc ttgactggta tccaagaaga attgacaaca gcgcttgttc ttgaagataa
     8341 ggacttggtt ctctttgtgg cggatacgct tgaagtggct aatgcaacac tgggtgccct
     8401 tcgtggacgt attgctaaag agcttggctt gattgataat gataagttca acttcctttg
     8461 ggtggttgac tggccgatgt ttgaatggtc tgaagaagaa ggccgctaca tgagcgccca
     8521 ccatcctttc acacttccac aggaagagac tgctcacgaa ttagaaggtg atttggctaa
     8581 ggttcgtgcc attgcttacg atatcgtctt gaacggttat gagcttggtg gtggtagcct
     8641 tcgtatcaac caaaaagacc ttcaagaacg catgttcaag gctcttggtt tctcaactga
     8701 agaagccaat gaccagtttg gtttccttct tgaagccatg gactatggtt tcccaccaca
     8761 cggtggtttg gctatcgggc ttgaccgttt tgtcatgttg cttgctggag aagaaaatat
     8821 ccgtgaagtc attgcctttc ctaagaacaa caaggcaact gacccaatga cacaagctcc
     8881 atcaacagtc gctctcaaac aactagagga actcagctta caagtagaag aagatgaaac
     8941 aaacaaaacg aattaagcgg tggcgctatt atctgcgccg ctttgcttat cagataaaaa
     9001 ttttacgtgt cttacaaagt atctctcgag aaaagtatga tgagaagatt tcggcctctc
     9061 tggtctatgg ttttttatca gcagtagcag ttaatttctt tttccaacca gggcatgtgt
     9121 attcgagtgg tgcaacaggt ctggcacaga ttatctctgc cttgagtaat cactggtttg
     9181 gttttcatat tccgatttcg ctaagctttt acgccattaa cttccctttg atggtcttag
     9241 cttggtatca gattggccat aagttcaccg tctttacctt tatcacggta tctatgagtt
     9301 ccttctttat ccagtttgtc cctgtggcaa ccttgacaga ggatcccatt atcaatgccc
     9361 tttttggggg tgttgttatg ggtttgggga ttggttttgc tcttcgaaac aatatctcca
     9421 gtggtgggac ggacatcgtc agcctgacta ttcgtaagaa aacgggtaag aatgtcggta
     9481 gtatttcttt cttggtaaat ggaactatca tgctgatagc aggtttgacc tttggttgga
     9541 aatacgctct ttattctatg attaccatct ttgtctctag ccgtgtgaca gacgcagtct
     9601 ttactaagca aaagcgtatg caggccatga ttgtgacaaa tcatccagag aaggtaattg
     9661 aaaaaatcca taaaaaattg caccgcggag caaccatgat ccacgatgca gaaggaacct
     9721 ataatcacga gagaaaggca gttttaatca ctgtcattac acgtgcagag tttaatgaat
     9781 ttaaacagat tatgacacaa gtggatccaa gctcctttgt ctctgtctcg gaaaatgttc
     9841 atattctagg aagatttgtt gagatagata attagtgatt aatactcttc gaaaatctct
     9901 tcaaaccacg tcagcgtcgc cttgccgtat atatgttact gacttcgtca attctatctg
     9961 caacctcaaa accatgtttt gagctgactt cgtcagtttc atctacaacc tcaaaaccat
    10021 gttttgagct gacttcgtca gtttcatcta caacctcaaa accatgtttt gagcaacctg
    10081 cggctagctt cctagtttgc actttgattt tcattgagta taaaaagatc ttgggaaaag
    10141 actcaatttc agtagaaaag gaagtaaatc ttcccacatt aaaacgcata gtatcaagtt
    10201 ttttcaatac ctgatactat gcgtttttgc ttttaacgac tttttgccca gtctcttatt
    10261 tttcgataat tttgtgggcg atcaattgac ggtaacaaat ttgtttattg tttttattat
    10321 cattaataat ctggagaata gtttcaaggg aaacacgacc aagctctaaa ctattgatat
    10381 cgacataggc tgccaagttg agcttgggat taaccgagtc aaagctgaga acagggacat
    10441 ccagctggtg tttggcaata tagttacaaa caccttcagc taggaggcta tcggttgtga
    10501 tgatagcatc aatttgtgga tcgtgcttga ataatcgctt gctaaattta tagccctttt
    10561 cttccagaaa ctcgtcggca aagtagatgc gattgttgtc agtggtaagt ttgtaatgtt
    10621 taagcgcctg ttcatagcct gttaaacggt ctttggtcac gaagagcttt ttacttcctc
    10681 cgataaaggc aatgcgtttg cagccttttt tgatgaaata ttcagtcgca tcaaaaccag
    10741 cttgaacatt gtcgttgtcg acaagtggga tgaaaggaga tagagattta cctaagataa
    10801 ggaaggggaa ctgttcttct gcgacgagtt ttacgagagg gtcttcttct tgggcataga
    10861 gaaagattag cccatctaca cgcttgccgt agaccatttg tgaaatagcg ttgagacgct
    10921 ccttctcatc tttccctgtt gctatctgaa tggcatagtg gttttcagat gcgacttgag
    10981 agatgccacg tagaaccgat ggaaagaaag gattctggta gaaggcgtct gagtcatcag
    11041 gaagaactaa tccgataacc tgagtatagc tgcttaccaa gctacgagcg ttgaggtttg
    11101 ggtggtagtt gagttccttc atagctttgc gaacgcgttt ttttgtttcg tcgctaatgg
    11161 ttgatttatt ttgaataaca cgggttacgg ttgaaggcga aacaccagca gccttggcca
    11221 cgtctttaat cgtaacgggc ataaaaatct cctatttatg gtaatttgga tcacggaaat
    11281 gtgttttata aaagatagtg accagataca gaacaaaaag aatattttgt acagtaatca
    11341 gggttgtcat attttggcca aataatccca aaataagtgt aatcagagtc ggcaatccta
    11401 aacagttcaa gataaagtga tagcactctt taaaggttct aaatgaaaag aggcgtgatt
    11461 tcttagtgat ataaaggaga agactagctc ctagagagac gataaagaaa ttcaaaccaa
    11521 agaggaagct cgcaccgaga actaggaaga ggctgatata gacacgattt tgttggtacc
    11581 agtctttaga aattgcttgg gtcaagctgt ctttgctttt gaaactctca gtctgaatgg
    11641 ctcggtaaga gatgcgggtc agttccttgc tttccttgct gatgactagc tcatttgtat
    11701 cgaaatgcag ttgcaagtcc ttaggtaatt ccttgatttg acttggacca atcacaacag
    11761 aaggggcttg actagctgtt ccagtataag ttaatgtgcc atcgacaatt gtagcatgtt
    11821 cagagagatc ctggacaacc tcatttgtca gaggttcata gacattatcg ataaaagttt
    11881 ctagcggata ggtctcctgg gagctgtttt ggatagcaat gggtaccata gacaagctga
    11941 taaggaagat actggtaaag agaagttgaa accagttgag ctcgaaacgt ttggacaggg
    12001 gcttacgaaa cccccaaata cttgaaaaat aggaaaatgg atatggaagc ataggtatct
    12061 ttctaaaagg tgttttctat atgagtatta ttatatagag aaatgcgctt tatttcaagt
    12121 ctaggagaaa aattgcttgc tacaaggata ttatttacag tgacaattgc taataatcaa
    12181 tgaaaatcaa agtgcaaact agccacaggt tgctcaaagc acaactttga ggttggagat
    12241 aaagctgacg tggtttgaag agattttcga agagtataaa atgaaaaagg gtggcgggga
    12301 taaattatcc cttgtcgcca ccacttgtaa gtcctgaaac aaagttcttt tgtaggaaga
    12361 agaagagaat acagattgga agggcgatga ggatagcacc tgctgagaag taggcaatct
    12421 tcaagttttt cgcattgtta acgaaggttt ggagacctac ggcaacagta aagtattctt
    12481 tctcacgaag caagaaacta gagaggatgt agtccccgaa aggtcccatg aaggcccaga
    12541 gagcttgtac ggcaaccatt gggcgaacaa gtggtagaac aatttgccag aagcggcgga
    12601 agtgtcctgc accgtctagt tttgcagatt cgtctaaaga cattggcact gtatcgaagt
    12661 agcctttcat gagccaagca ttcatcggga taccaccacc aacgtagagg aagatgagga
    12721 accagttgtg gttaagggcg ttcaacataa gcgccataac gaagaaggct gtcaaagcgg
    12781 ccatagttgg caccatttgg atgatcaaga agaagaccaa actttgttta cgagccaaga
    12841 agttgtaacg gctgtaagca taaccagcaa gtacgatgat acttgtttga acagccatgg
    12901 taattaaggc gataatcaaa gtgttgaggt accaagtacc gtacaaggtt tcagtgaaga
    12961 ggcctttaaa gttatcaaaa ttgaggtcga tattagtatc tagtttaaag gctgagacgt
    13021 tacctgcttt aaaggctgac ataatggtaa tcaacagtgg atagataatt acaattgata
    13081 gaccaatcag gtaaaggtaa gtaaggcttt gagtcagtct acgtttgagt ttaattgagt
    13141 tattcatctt agacgtcctc catatcaaat gcgtgtagtt tcttgaatgc gatcatagag
    13201 attgagatga caatgataga gataatcaag gtaacagctg ccgccattga gtattgagga
    13261 gatgtacctg ttgtcaaacg gtagatccat gagatcaaga tatcggttga accagctcca
    13321 cctccgacac taccaggtcc tccaccattg aagaggtaca tgatagagaa gttgttaaag
    13381 ttgaaggtgt attggctaat caaagtaggt gccgcaacag ccaaaatcat tgggaaagtg
    13441 atgttgcgga atttttgcca agcgttggca ccgtcaatat aagctgcttc gtaaaggtcg
    13501 ttaggaatag attgcaagat acccaaagtc aaaacgtaga tgtatgggaa tccgagccaa
    13561 ccttgcatca taatcaaggc aatcttagtc caagttgggt ctgttttcca aggaataaga
    13621 gctccatcaa ggaaaggaag gaatttagcc aagattggca atacttgagt gttgatagca
    13681 ccgacactat cgttaaacat atttgagaat gtcaagatag tgatgaaggc tgggacagcc
    13741 caaggaagaa ggaaaataac accaaagata cgttttcctt tgataaatgg ttggttggca
    13801 atgatagctg tgaagatacc aattacgatt tgtaaagtag aagctgccaa agcccaaatg
    13861 atagtccaag aaagaacaga accaaaggca gaacggaagg tactcaagct ccaaatgttt
    13921 gtaaagttgg tcaaaccaac ccagtccaac aacttgtttg gtggcaagtg ttggaagtcg
    13981 tagttggtaa aggcgatcat caaggttacg ataactggga agataatcgc gaaggtcatg
    14041 gcaacataag atggaatgat caagaggtaa gggaagccat tttcataaat ccctttgatc
    14101 atgtctttga gtgtgcgtgg aactggaatt ccattgttaa tgcgttttgc aatcgtatgt
    14161 gcatctttga tatttgagaa ataaaagagt acataaacga ttacaaagat tagatggaag
    14221 gcaccacgaa tcagcataaa gagggaatta tcacgacctg gtttgtcacc aagagtgatg
    14281 aggttgctca attcaggggt tgcaagtgct aggaagtaaa ggacaaatac gatggttaca
    14341 ccaaggaaga taaaaccttt ggctttttgt ttattgtaaa tctgtcctaa cccaggaatg
    14401 atagacagca gggctgcttt actaggttgt tgcttttcca tattaactcc tttcatagga
    14461 tactactttt ttataagaaa gctaaattat ttgtgtctct attgataaat tcaaaggggg
    14521 atttgatttc cacccccctt gaacaaattt tttattcacc aaatttttgt ttgattgttt
    14581 ctttgatcaa tgttacagca tcgttagcag ctgttttagc atctttttga ccacttacag
    14641 catcaaagag catatttttc gctggatccc aaactgcaga catttgagag atgtttggca
    14701 gtggttgagt gttcttgaac tgtttgataa cagctgttgt caactcatcg tttttacctt
    14761 cagcgtatga acgagcctca gtattagctg ggatttcgtt agtcttatca tataatactt
    14821 tttgttgttc agttgcaaca aggaagtcta caaatttttg agaagcttca aggttcttaa
    14881 cggcttgagg aatgacccaa gctttaccac caccgaatgc agcatattct tttccatttg
    14941 gaagagttgg gatagttgca actccgtagt ttactttagc atctttaaag gcttgagctt
    15001 tccaaggtcc gtcgatgata gcagctgttt taccttcttg gaattgagtt tggattaagt
    15061 ttccagcacc ttctgtatct tgcatacctt taggccattt ttcgtaccaa gatttagcgt
    15121 agttgatacc tgcgatagaa ccgtcgtttg caagaccgat gtctttagcg tctttaccgt
    15181 tttggccaaa gacgtaagca ccgttaccgg caagaagtcc atatgtatag tagaagtttg
    15241 tccagtcagc taggaaggca gtagttttac catcttcacc agcgaatgcg tatttgctat
    15301 ctttagcaag gttttccaag tcagcaaatg tttttggagc atctttcacc aagtctttgt
    15361 tgtagtacat aacaagtgac tcgataacgg caggagcacc gtaaacttta ccattagcag
    15421 ctgttacaag agatttagtt gtgtcgtctg ttttagcacc atcgctcaat ttcacttctg
    15481 aaagttgtcc gtcagaacca aggctaccta cacggtcgta tggagccatc ataacatcag
    15541 ggacattacc agattggttg tcaagagaaa gtttatcaag acctcctaga gcatcaccag
    15601 ttttaagagt gacttttact ccagcttctt tttcataagc tttagcaacc tcttcaatat
    15661 agctcttata tccctcgtct acatatacag tgagttcttt gacttcagat gaaccagaat
    15721 cagcaggctt atcagcagtt ttgcttccgc aagctaccaa aagcaagcta gcaagtgtaa
    15781 cagttccaag caccgcagtg ctcttcataa atttagatga catagtgtat tcctcccaaa
    15841 gaatagcaag ttttattgat aaggaaacgc aaacgttttc ctttatgagc ttagtatagc
    15901 acaaatagaa aacggttgca agtatttttt gtaaaaattt taaaaaattt ttaagcttga
    15961 tttgatagca taattttgca ttttaataga agaaaagtat gaaatagata agaatacagt
    16021 atttttaata aaaatatatg gaatctgtct aaagagggaa atcaggaggg gctacctccc
    16081 tgtttctaat ccctatctat tttctataga cgtttgtgct ttgttgcata taaattcaag
    16141 ctccctaagg aggttttgta ctttgtagac tgtctcgtga gagtcaaact ttaggattag
    16201 atagttttaa gttatctttg ctaaaatgag ttagtctcct taaaaataat caaaaaagtt
    16261 ttttaaaaac gcttgcaatt atgcttgaaa aggagtatac ttataagtaa cgcaaacgtt
    16321 tgcgtctgca aaatacgcaa cgttccatta ttttaacaca cgaggtgcta ttatgaaaaa
    16381 acgtcaaagt ggtgtgttga tgcacatctc ttctcttcca ggagcttacg gaatcggatc
    16441 atttggtcaa agtgcttacg acttcgttga tttcttggtc cgtacaaaac aacgttactg
    16501 gcaaatcctt ccattaggag caactagtta cggggattct ccttaccaat ctttctcagc
    16561 cttcgcagga aacactcatt ttatcgattt agatatcttg gtggagcaag gtttgttggg
    16621 agcgagtgac cttgaaggag ttgactttgg tagcgatgcg tctgaagttg actatgctaa
    16681 aatctactat gcacgtcgtc ctcttttaga aaaagcggtg aaacgtttct ttgaagtcgg
    16741 agatgttaaa gattttgaga aatttgctca agacaaccaa tcatggcttg agctctttgc
    16801 tgagtatatg gctatcaaag agcattttga caatcttgct tggactgaat ggccagatgc
    16861 agatgctcgt gctcgtaaag cttcagcact tgaaagctat cgtgagcaat tggcagacaa
    16921 gttggtttac caccgtgtga ctcaatactt cttcttccaa caatggttga aattgaaagc
    16981 ttacgctaac gacaaccaca ttgaaatcgt tggggacatg ccaatctacg tagcggaaga
    17041 ttcaagtgat atgtgggcaa atccacatct cttcaaaaca gatgtcaatg gtaaggctac
    17101 ttgtatcgca ggatgcccac cagatgagtt ttctgtaact ggtcagcttt ggggtaaccc
    17161 aatctatgac tgggaagcaa tggacaaaga cggctacaaa tggtggattg aacgcttgcg
    17221 tgaaagcttc aaaatctacg atatcgttcg tatcgaccac ttccgtggct tcgaatctta
    17281 ttgggaaatc cctgctggtt ccgatacagc agcacctggt gagtgggtga aaggtccagg
    17341 ctacaagctt tttgcagccg ttaaggaaga acttggtgag ctaaacatca tcgcagaaga
    17401 ccttggcttc atgacagatg aagtgatcga attacgtgaa cgtactggct tcccaggaat
    17461 gaagattctt caatttgcct tcaacccaga agacgaaagc attgatagcc cacacttggc
    17521 acctgctaac tcagttatgt acacaggaac acacgataac aatacggttc ttggttggta
    17581 ccgtaatgag attgatgatg cgactcgtga gtacatggct cgttacacga accgtaaaga
    17641 atacgaaaca gtggtacacg ctatgcttcg tacagtattt tcatcagtta gctttatggc
    17701 aattgcaact atgcaagatt tactagaatt ggatgaggca gctcgtatga acttcccatc
    17761 tacccttggt ggaaactggt cttggcgtat gactgaagat caattgacac cagctgtcga
    17821 ggaaggtttg cttgacttga caacaattta tcgccgaatt aatgaaaatt tggtagattt
    17881 aaagaaataa gacaataatc aggagacaac taaacatgtt atcactacaa gaatttgtac
    17941 aaaatcgtta caataaaacc attgcagaat gtagcaatga agagctttac cttgctcttc
    18001 ttaactacag caagcttgca agcagccaaa aaccagtcaa cactggtaag aaaaaagttt
    18061 actacatctc agctgagttc ttgattggta aactcttgtc aaacaacttg attaaccttg
    18121 gtctttacga cgatgttaaa aaagaacttg cagctgcagg taaagacttg atcgaagttg
    18181 aagaagttga attggaacca tctcttggta atggtggttt gggacgtttg gctgcctgct
    18241 ttatcgactc aattgctact cttggtttga atggtgacgg tgttggtctt aactaccact
    18301 ttggtctttt ccaacaagtt cttaaaaaca accaacaaga aacaattcca aatgcatggt
    18361 tgacagagca aaactggttg gttcgctcaa gccgtagcta ccaagtacca tttgcagact
    18421 ttactttgac atcaactctt tacgatattg atgttactgg ttatgaaaca gcgactaaaa
    18481 accgcttgcg tttgtttgac ttggattcag ttgattcttc tattattaaa gatggtatca
    18541 actttgacaa gacagatatc gctcgcaact tgactctctt cctttaccca gatgatagtg
    18601 accgtcaagg tgaattgctc cgtatcttcc aacaatactt catggtttca aacggtgcgc
    18661 aattgatcat cgacgaagca atcgaaaaag gaagcaactt gcatgacctt gctgactacg
    18721 cagttgtcca aatcaacgat actcacccat caatggtgat tcctgaattg attcgtcttt
    18781 taactgcacg tggtatcgag cttgacgaag caatctcaat tgttcgtagc atgactgcct
    18841 acactaacca cacaatcctt gctgaagcgc ttgaaaaatg gcctcttgaa ttcttgcaag
    18901 aagtggttcc tcacttggta ccaatcatcg aagaattgga ccgtcgtgtg aaggcagagt
    18961 acaaagatcc agctgttcaa atcatcgatg agagcggacg tgttcacatg gctcacatgg
    19021 atatccacta cggatacagt gttaacgggg ttgcagcact ccatactgaa atcttgaaaa
    19081 attctgagtt gaaagccttc tacgaccttt acccagaaaa gttcaacaac aaaacaaacg
    19141 gtatcacttt ccgtcgttgg cttatgcatg ctaacccaag attgtctcac tacttagatg
    19201 agattcttgg agatggttgg caccatgaag cagatgagct tgaaaaactt ttgtcttatg
    19261 aagacaaagc agctgtcaaa gaaaaattgg aaagcatcaa ggctcacaac aaacgtaaat
    19321 tggctcgtca cttgaaagaa caccaaggtg tggaaatcaa ttcaaattct atctttgata
    19381 tccaaatcaa acgtcttcac gagtacaaac gccaacaaat gaacgctttg tacgtgatcc
    19441 acaaatacct tgacatcaaa gctggtaaca tccctgctcg tccaatcaca atcttctttg
    19501 gtggtaaagc agctccagcc tacacaatcg ctcaagacat tatccattta atcctttgca
    19561 tgtcagaagt tattgctaac gatccagcag tagctccaca cttgcaagta gttatggttg
    19621 aaaactacaa cgttactgca gcaagtttcc ttatcccagc atgtgatatc tcagaacaaa
    19681 tttcacttgc ttctaaagaa gcttcaggta ctggtaacat gaaattcatg ttgaacggag
    19741 ctttgacact tggtactatg gacggtgcta acgtggaaat cgctgagttg gttggagaag
    19801 aaaacatcta catcttcggt gaagattcag aaactgttat cgacctttac gcaaaagcag
    19861 cttacaaatc aagcgaattc tacgctcgtg aagctatcaa accattggtt gacttcatcg
    19921 ttagtgatgc agttcttgca gctggaaaca aagagcgctt ggaacgtctt tacaatgaat
    19981 tgatcaacaa agactggttc atgactcttc ttgacttgga agactacatc aaagtcaaag
    20041 agcaaatgct tgctgactac gaagaccgtg acgcatggtt ggataaagtc atcgttaaca
    20101 tttctaaagc aggattcttc tcatctgacc gtacaatcgc tcagtataat gaagacatct
    20161 ggcacttgaa ctaatactct tcgaaaatct cttcaaacca cgtcagcttt atctgcaacc
    20221 tcaaagcggt gctttgagca acctgcggct agcttcctag tttgctcttt gattttcatt
    20281 gagtataaga tacaaattta tactaataca ttttgtaaaa aagcgagttt cgattgaaat
    20341 tcgctttttt aaatgctgtg gatttgggtc aatcttgtct aaaaataggg aaatcctaga
    20401 tacagtgaag gctttaaatg ctggttttta ctgtcttcag ccttatattt tttcgtagtt
    20461 ggttacctca catctcttgt attcgcttac ataaagtatt ataatataat tgtaggaaag
    20521 aaggtgtttt tatgatatac acacttaaat tggtgttgtt tattaccttt cttgtaataa
    20581 gcttgttacc tgataagatt tttggaaaaa ataaaaaaat ttggaaaata gtttttgcaa
    20641 tattgacggc agtggcagca ttgtcattta tgtactaagt tattttaaga atgtagggaa
    20701 ataaacccta cattcttttt agttttttct gttttctaaa ttctatttat ccaagcgatt
    20761 caacatttct tgcttcttcg cttcaagttc tgcacgcttt tcttcgattt cggcatgttt
    20821 tttctcgagt tcagaacaac ttgcaccatt gctaaattct tttcgccatc aggagatagg
    20881 gtgagtcgac atgtctatta ctcacccaaa gcagtcctac aaagcaggaa ttttctgtta
    20941 cttttttgga aatagtaacg tttatacagc tttgacactt cgtatcaaag cgccaaacac
    21001 actccgaggg atttacagaa agcagaaaag gaatgatctg gtataagatc attccttttc
    21061 tctctttttc tttaagtaat tatatacaat gtacgacgaa gtcgtcattg caatgctgat
    21121 ccaccaccta aagggaactt taaacaacat tgataagata aagaatataa acaacgaaaa
    21181 tacgttatac ccaattaatt ttattgtata tctcatgatt aaaagttaat ccttccgttg
    21241 ttaggaatgg catcattttt atcccataat tgtgctaaat aagtccccgg tgataataaa
    21301 ttcatagcga attctaaagc aacatcattt acaaaccaac tacctagata tctagaaatt
    21361 gctgaacgaa tagcactttt tgctgcatgt tttcctttta ctttaattag atttgcaagg
    21421 cctgcagtag ttcctcctaa tgctaaagct attgcagtat ctaatagagc acccatttga
    21481 ttaactgtaa taccttgcca agctgctcta aatggagagt atgtaggtgg gattgtataa
    21541 tcgccttgta attgtcggtt aattacttct ttgatccatt gttgtgagac gtctggatga
    21601 aaagattgga tttcgtttgc aagtgtattg atttgttctt ctgttagaga agtgacaggt
    21661 tgaagttcca tatttgtttc aatttgtgat acttgttcag aagcgtatac agctgaaaca
    21721 cttggaatcg ctgatacaat taacacaatt gacgtcaaaa aaaccgaaat aaatttcatt
    21781 aatttgttca tgagcttttc tcctttttat ttgtatctgc ttacat
//
LOCUS       DAVMGZ010000019        21499 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000019 DAVMGZ010000000
VERSION     DAVMGZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21499
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.020"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            86..2308
                     /gene="recJ"
                     /locus_tag="VYQ93_000612"
     CDS             86..2308
                     /gene="recJ"
                     /locus_tag="VYQ93_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV3335347.1"
                     /translation="MITPTYEWQFALQVENADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSIPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQILEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEVSFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMMDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2483..4144
                     /locus_tag="VYQ93_000613"
     CDS             2483..4144
                     /locus_tag="VYQ93_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV3335348.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4213..4992
                     /locus_tag="VYQ93_000614"
     CDS             4213..4992
                     /locus_tag="VYQ93_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV3335349.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNFAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5104..6324
                     /gene="murM"
                     /locus_tag="VYQ93_000615"
     CDS             5104..6324
                     /gene="murM"
                     /locus_tag="VYQ93_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV3335350.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMIWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6329..7561
                     /gene="murN"
                     /locus_tag="VYQ93_000616"
     CDS             6329..7561
                     /gene="murN"
                     /locus_tag="VYQ93_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV3335351.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQDLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7640..8341
                     /locus_tag="VYQ93_000617"
     CDS             7640..8341
                     /locus_tag="VYQ93_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV3335352.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDHWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8381..10228
                     /gene="uvrC"
                     /locus_tag="VYQ93_000618"
     CDS             8381..10228
                     /gene="uvrC"
                     /locus_tag="VYQ93_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV3335353.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGSLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10218..11051
                     /locus_tag="VYQ93_000619"
                     /pseudo
     CDS             10218..11051
                     /locus_tag="VYQ93_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(11235..12035)
                     /locus_tag="VYQ93_000620"
     CDS             complement(11235..12035)
                     /locus_tag="VYQ93_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3335354.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12206..12811
                     /locus_tag="VYQ93_000621"
     CDS             12206..12811
                     /locus_tag="VYQ93_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV3335355.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            12824..14224
                     /gene="pepV"
                     /locus_tag="VYQ93_000622"
     CDS             12824..14224
                     /gene="pepV"
                     /locus_tag="VYQ93_000622"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV3335356.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14278..14856
                     /locus_tag="VYQ93_000623"
     CDS             14278..14856
                     /locus_tag="VYQ93_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV3335357.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15016..15168
                     /locus_tag="VYQ93_000624"
     CDS             15016..15168
                     /locus_tag="VYQ93_000624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV3335358.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            15211..15303
                     /locus_tag="VYQ93_000625"
     CDS             15211..15303
                     /locus_tag="VYQ93_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV3335359.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15474..16799
                     /gene="brnQ"
                     /locus_tag="VYQ93_000626"
     CDS             15474..16799
                     /gene="brnQ"
                     /locus_tag="VYQ93_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV3335360.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16897..17934
                     /locus_tag="VYQ93_000627"
     CDS             16897..17934
                     /locus_tag="VYQ93_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV3335361.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDTRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            17936..18397
                     /locus_tag="VYQ93_000628"
     CDS             17936..18397
                     /locus_tag="VYQ93_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV3335362.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            18415..19131
                     /locus_tag="VYQ93_000629"
     CDS             18415..19131
                     /locus_tag="VYQ93_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV3335363.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            19254..19679
                     /gene="rplK"
                     /locus_tag="VYQ93_000630"
     CDS             19254..19679
                     /gene="rplK"
                     /locus_tag="VYQ93_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV3335364.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            19887..20576
                     /gene="rplA"
                     /locus_tag="VYQ93_000631"
     CDS             19887..20576
                     /gene="rplA"
                     /locus_tag="VYQ93_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV3335365.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(21168..21314)
                     /locus_tag="VYQ93_000632"
     CDS             complement(21168..21314)
                     /locus_tag="VYQ93_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335366.1"
                     /translation="MKTKEQTRKLAAGCSKHCFEVVDRTDEVSNHTHGKATLTWFEEI
                     FKEY"
BASE COUNT         6450 a         3970 c         4968 g         6111 t
ORIGIN      
        1 ttataaaaaa gcaatctgga tagagaaaac gtctaaatca tgttataatg aagcaataga
       61 attcttagaa agagtggatg tctttttgat aacacctact tatgaatggc agtttgccct
      121 gcaggtagaa aatgcggatt ttacaaagat agccaagaag gctggactgg gtcctgaggt
      181 ggctcggtta ttgtttgaga gagggattca ggaccaagaa agtctgaaga agtttttaga
      241 accttccttg gaggacttac atgatgctta tctgctccat gatatggaca aggcagtgga
      301 gcggattcgt caggctattg aagaagggga aaatattctc gtttatggag actatgatgc
      361 ggatggcatg acttcggctt ctattgtgaa ggaaagtttg gaacaacttg gtgcggagtg
      421 ccgagtttac ctgccaaatc gttttaccga tggctatggc cctaatgcta gtgtttataa
      481 atactttatc gagcaagaag gaatttcctt gattgtgacg gtggacaatg gggttgctgg
      541 tcatgaggct attgcattgg ctcagtctat gggagtagat gtcattgtga cagaccatca
      601 ttccatacct gaaaccctgc cagatgctta tgctattgtc catcctgaac atccagatgc
      661 ggattatcct tttaaatatt tggctggttg tggagttgct ttcaagttgg cttgtgccct
      721 gttagaagaa gtgcaagtgg aattgcttga tttggtcgct attggaacta ttgcagatat
      781 ggtgagtctg acggatgaaa atcgtatctt agttcaatat ggtctggaaa tgttgggtca
      841 tacccagcgc attggtctgc aagaaatgct ggacatggct gggattgctg ctaacgaagt
      901 aacagaagaa acggttggtt tccagattgc tcctcgtttg aatgccttgg gtcgcttgga
      961 tgatcccaat cctgccattg atttgttgac tggatttgat gatgaggaag cgcatgagat
     1021 tgcccttatg attcaccaga aaaacgaaga gcgcaaggaa atcgttcagt ctatctatga
     1081 agaagccaag accatggtgg atcctgagaa gaaggttcag gtcttggcca aggaaggctg
     1141 gaatcctggg gttctaggaa tcgtggctgg tcgtttattg gaagaattgg gacagacagt
     1201 cattgttctt aatatagaag acggtcgtgc caagggcagt gctcgtagtg tggaagcggt
     1261 cgatattttt gaagctctgg atccctatcg agacctcttc atcgcctttg gaggtcatgc
     1321 aggtgcagcg ggtatgacgc tggaagttga gcaactctca gatttatctc agattttgga
     1381 agattatgtt cgtgaaaaag gtgcagatgc tggtggcaag aataagttaa acctagatga
     1441 agagttggat ttggaggcac ttagcttgga aacggtcaaa agttttgaac gtttagctcc
     1501 ttttggaatg gataatcaga aacctatttt ttatatcaag aattttcagg tcgaaagtgc
     1561 tcgtactatg ggggcaggta atgcccatct aaagctgaaa atttccaagg gtgaggtgag
     1621 ttttgaagtg gtagcctttg gtcaaggcag atgggcgaca gagttttctc aaaccaagaa
     1681 tctagagtta gcggttaaat tgtctgtcaa ccaatggaac ggccaaactg ccctccagtt
     1741 gatgatgatg gatgcgcgag tggaaggtgt tcaacttttt aacattcgtg gaaaaaatgc
     1801 agtcttgcca gaaggtgttc cagtcttgga ttttcctgga gaactgccaa atcttgcggc
     1861 tagtgaagct gttgtcgtaa aaaacattcc agaggatatt actcagctga agaccatttt
     1921 tcaggaacag catttctctg ctgtctattt caaaaatgat attgacaagg cctattatct
     1981 gacaggttat gggactagag atcagtttgc caaattgtac aagactattt accagttccc
     2041 agagtttgat attcgctaca agctgaaaga tttggctgca tatcttaata ttcaacaaat
     2101 cttgctggtc aagatgattc aagtatttga agaactaggc tttgtgacga taaaagatgg
     2161 tgtgatgaca gtcaataaag aggcgccaaa gcgggagata ggagaaagtc aaatttacca
     2221 aaatctcaaa caaaccgtta aagaccaaga aatgatggcg ctgggtacgg tgcaagaaat
     2281 ttatgatttt ttgatggaaa aagagtagaa gttaggaaag agttgggaaa tcaactcttt
     2341 tttgaaaaca gaccttcatt ttgaaaatca tcaaaaaaat ggtataatgg taggaaaaga
     2401 ttcggctgaa agtattagaa cttttagaat aagagggtag agttgcccta taatcaagat
     2461 aaactaagat tttggaggaa aaatgagtaa tatcagttta acaacacttg gtggtgtgcg
     2521 tgagaatgga aaaaatatgt acattgctga aattggagag tccatttttg ttttgaatgt
     2581 agggttaaaa tatcctgaaa atgaacaatt aggggtcgat gtggtgattc caaacatgga
     2641 ttaccttttt gaaaatagcg accgtattgc tggggttttc ttgacccacg ggcatgcgga
     2701 tgccattggt gctctacctt atctcttggc agaggctaaa gttcctgtat ttgggtctga
     2761 gttgaccatt gagttggcaa agctctttgt caaaggaaat gatgccgtta agaaatttaa
     2821 tgatttccat gtcattgatg agaatacgga gattgatttt ggtgggacag tggtttcctt
     2881 cttccctacg acttactccg ttccagagag tctgggaatt gtcttgaaga catcggaagg
     2941 aagcatcgtt tatacaggtg acttcaaatt tgaccaaacg gctagtgaat cttatgcaac
     3001 tgattttgct cgtttggcag agattggtcg tgacggcgtc ctggctctcc tcagtgattc
     3061 ggccaatgca gacagcaata ttcaggtggc tagtgaaagt gaagttaggg atgaaattac
     3121 ccaaactatt gctgactggg aaggtcgtat catcgttgca gctgtttcca gtaatctttc
     3181 tcgtattcag cagatttttg acgctgcgga taaaacaggt cgacgtatcg tcttgacagg
     3241 atttgatatt gaaaatatcg tccgcacagc gattcgtctt aagaagttgt ctttagccaa
     3301 cgaaattctc ttgattaagc ctaaagatat gtctcgcttt gaagaccatg agttgattat
     3361 tcttgagaca ggtcgtatgg gtgaacctat caatggactt cgtaagatgt cgattggtcg
     3421 ccatcgttat gtagaaatca aggatgggga cctggtctat attgctacgg ctccgtctat
     3481 tgctaaagaa gcctttgttg cgcgtgtgga aaatatgatt tatcaggcag gtggggttgt
     3541 caaattgatt acccaaagtt tacatgtatc agggcacgga aatgtgcgtg atttgcagct
     3601 gatgatcaat cttttgcaac ctaagtacct cttccctgtc caaggagagt atcgtgagtt
     3661 ggatgctcac gctaaggctg ccatggcagt tgggatgttg ccagaacgca tcttcattcc
     3721 taaaaagggg acgaccatgg cttacgagaa tggagacttt gttccagctg gatcggtttc
     3781 agcaggagat atcttgattg atgggaatgc cattggtgat gttggaaatg ttgttcttcg
     3841 tgaccgtaag gtcttgtcag aggatggaat tttcatcgtg gctattacag tcaaccgtcg
     3901 tgagaagaaa attgtggcta gggctcgtgt tcatacgcgt ggatttgttt atctcaagaa
     3961 gagtcgcgat attctacgtg aaagttcaga attgattaac caaacggtag aagattatct
     4021 tcaaggagat gactttgact gggcagatct taaagggaag gttcgagata atttgaccaa
     4081 gtatctcttt gaccaaacca agcgtcgtcc agctatttta ccagtagtca tggaagcaaa
     4141 ataatcgttg aaataaacag agagaaagtc gagtttcggc tttttcttat agaaaaatag
     4201 aaggagaaaa tcatggcagt aatgaaaatc gagtattact cacaagtatt ggatatggag
     4261 tggggggtga atgtcctcta ccctgatgcc aatcgagtgg aagaaccaga gtgtgaagat
     4321 attcccgtct tgtacctttt gcacgggatg tctggaaatc ataatagttg gcttaagcgg
     4381 accaatgtag aacgcttgct tcgaggaact aatctcatcg ttgttatgcc caataccagc
     4441 aatggttggt ataccgatac ccagtatggt tttgactact acacggctct agcagaggaa
     4501 ttgccacagg ttctgaaacg cttcttccct aatatgacta gcaagcgtga aaagaccttt
     4561 atcgctggtc tttctatggg aggctacggc tgcttcaaac tggctcttgc gacaaatcgt
     4621 ttttctcatg cagctagttt ttcaggtgcc ctcagctttc aaaacttttc tcctgaaagt
     4681 caaaatctgg gaagtccagc ctactggaga ggtgtttttg gagagattaa agactggaca
     4741 actagtccct attctcttga aagtctggct aaaaaatcgg ataaaaagac caaactttgg
     4801 gcgtggtgtg gcgaacagga tttcttgtac gaagccaata atttcgcagt gaaaaatctc
     4861 aaaaaactag gttttgatgt gacctatagc catagcgctg gaactcacga gtggtactac
     4921 tgggaaaaac aattggaagt ttttttaaca accctaccaa ttgatttcaa attagaagag
     4981 agactgactt agtttgaact tcagcatagg gggagtagaa ctaaaataaa atatgttttc
     5041 actagacttt tcaaacgaaa gtagtagaat agtaataaaa tactggagga aagagagtag
     5101 gaaatgtacc gttatcaaat tggcattccc acattagaat atgatcagtt tgtcaaagaa
     5161 catgaattag ccaatgtatt acaaagtagt gcttgggagg aagttaagtc taattggcaa
     5221 catgagaagt ttggtgttta cagggaagaa aaattactgg cgacagctag tattttgatt
     5281 agaactcttc cgctaggcta taaaatgttt tacatcccaa gaggacctat attggattat
     5341 ggggataaag aactcttgaa ttttgccatt cagtctatta agtcctatgc tcgcagtaag
     5401 agagcggttt ttgtgacttt tgacccaagt atttgcctat ctcaaagttt aatcaatcag
     5461 gaaaagacag aatttcctga aaatctggct attattgata gtttgcaaca aatgggagta
     5521 aggtggtcag gaaaaacgga ggaaatggga gacaccattc aacctcgtat tcaggcgaaa
     5581 atatacaagg aaaattttga agaagataaa ctttccaagt caacaaaaca ggctattcga
     5641 acagcacgaa acaaagggct tgagattcaa tatggtggac tggaactatt agattcattt
     5701 tcggagttga tgaaaaaaac tgagaagcga aaagagattc atttgaggaa tgaagcctat
     5761 tataaaaaat tgttagataa ttttaaggac aaggcctata tcaccttggc caccttggat
     5821 gtttctaaac gttcgcaaga gttagaagaa cagttagcga aaaatagagc cttggaagag
     5881 acctttactg agtcgactcg aacttcaaaa gtagaagcgc agaagaagga aaaagaacgt
     5941 ttgttagagg aattgacctt cttgcaggaa tatatagatg taggtcaagc gagagttcct
     6001 ttagcggcta ctttgagttt ggaatttggt actacctctg tcaatatata tgctggtatg
     6061 gatgatgatt ttaaacgtta caatgcacca attttaactt ggtatgaaac ggctcgctat
     6121 gcctttgaac gaggtatgat ctggcaaaat ttaggtggtg ttgaaaactc tctcaatggt
     6181 ggactttatc attttaagga aaaatttaat ccaacgattg aagaatactt gggtgaattt
     6241 acaatgccca ctcatcctct ctatcctctg ttaagacttg ctcttgattt ccgtaaaaca
     6301 ttaagaaaaa aacatagaaa gtaagtatat ggcactaaca acactcacga aagaagagtt
     6361 tcagacttat tctgatcagg tttcttctcg ttcctttatg caatctgtcc agatggggga
     6421 tttgctagaa aaaagagggg ctcgaattgt ttatcttgct ttgaaacaag aaggagaaat
     6481 tcaagttgca gctctggttt atagcctgcc catgctgggt ggtctgcata tggaactcaa
     6541 ttcggggccg atttataccc aacaagatgc tcttccagtt ttttatgcag agttaaaaga
     6601 atatgccaag caaaatggtg tattagagtt gcttgtaaaa ccctatgaaa cttatcaaac
     6661 ttttgatagc caaggtaatc caatagatgc tgagaaaaaa agtattattc aagatttgac
     6721 tgatttaggt tatcaatttg atggcttaac aacaggttac ccaggtggag aaccagattg
     6781 gttatactat aaagatttaa ctgaattaac tgaaaagagt ttgcttaaaa gttttagcaa
     6841 aaagggtaaa cccttggtga aaaaggctga aacctttggc attcggttga aaaagttaaa
     6901 acgtgaagaa ctatcgattt ttaagaatat aacaaaagaa acctctgaac gtagagaata
     6961 tagtgataaa agtttagaat attatgagca tttttatgat acttttggag aacaagcgga
     7021 gtttctcata gcaagcttaa atttttcgga ctatatgagc aaattgcaag gtgaacaaag
     7081 taaactagaa gaaaacttgg acaagttgcg acttgatttg agtaaaaatc ctcattctga
     7141 gaaaaaacaa aatcaactga gagaatattc tagtcaattt gaaacgtttg aagttcgaaa
     7201 agcagaagcg cgagacttga ttgaaaaata tggagaagaa gatattgttt tagctgggag
     7261 tttatttgtt tatatgcctc aggaaacgac ttatctcttt agtggttcct acactgagtt
     7321 taataagttc tatgcccctg cactgcttca aaaatatgtt atgttggaaa gcataaaacg
     7381 tggaatacct aaatacaact tcctaggcat tcaagggatt tttgatggaa gtgatggtgt
     7441 tttgcgtttt aaacagaatt ttaatggcta tattgtacgc aaagcaggta ctttccgtta
     7501 ccatccatcg cctttaaaat acaaagctat ccagttactc aaaaaaatag taggacgtta
     7561 agatgaaaaa gtcagtattt agatttcttt tagcttcttt tagtaaaata attcttattt
     7621 gctagaaagg tggagagaca tgcgctggct ttttcgtttg ataggggctt tcttttcttt
     7681 tgtgtggcgt ttgttttggc gtctggtttg gatagttgtg ctcttatgtg tgcttgcttt
     7741 cggacttctc tggtatctga acggagattt tcaaggagcg ctaaagcaag cagaacggtc
     7801 agtaaaaatt ggtcaacaaa gtattgacca ctgggagaaa acagggcaac tgcctaagtt
     7861 aagccagaca gatagtcacc agcattctga aggaaggtgg gcacaggcct ctgctcgtat
     7921 ttacctggat ccgcagatgg attcacgctt tcaagaggct tatttagaag caatccagaa
     7981 ctggaatcaa actggtgctt ttaactttga actcgtgact gagtctagta aggcggatat
     8041 tacggctacg gagatgaacg acggaggcac tcctgtggca ggagaggcgg aaagtcaaac
     8101 taatctctta acagggcaat tcttgtccgt aacggtgcgg ttgaatcatt attatttgtc
     8161 caatccatac tatggctact cctatgaacg ccttgtccat acggcagaac atgagttagg
     8221 tcatgcgatt ggcttggacc atacagatga gaagtctgtc atgcaaccag caggttcctt
     8281 ttatggtatc caggaagagg atgttgcaaa cctccgaaaa atatatgaga ctagtgagta
     8341 gggtactatc tttccctact ttttttgcta taatggaact atgaacaact tgattaaatc
     8401 aaaactagag ctcttgccga ccagccctgg ttgctacatt cataaggata aaaatggcac
     8461 cattatctat gtaggaaagg ctaaaaatct gcgtaatcga gtacggtcct attttcgtgg
     8521 aagtcatgat accaagacag aggctctggt gtctgaaatt gtggattttg aatttattgt
     8581 tacggagtct aatattgagg cacttctcct agaaatcaac ctgatcaagg aaaacaagcc
     8641 caagtacaat atcatgctca aggatgacaa gtcctatcct ttcatcaaaa tcaccaatga
     8701 gcgctatcca cgcttgatta tcactcgtca ggtcaaaaag gacggaagtc tttattttgg
     8761 accctatccc gatgtggggg cagccaatga aatcaagcgg ttgctggatc ggatattccc
     8821 ttttcgtaag tgtaccaacc cgccctctaa ggtctgtttt tattaccata tcggtcaatg
     8881 catggcccac accatctgta agaaggatga ggcttatttc aagtctatgg cccaggaggt
     8941 ttctgatttt ctgaaagggc aggataacaa aatcattgat gagctaaagg gcaaaatggc
     9001 agcggcagcc cagactatgg agtttgaacg tgcggcggaa taccgtgacc tgattcaggc
     9061 tattggaacg cttcgaacca agcaacgggt catggctaag gatctccaaa atcgcgatgt
     9121 ctttggctac tatgtggata agggctggat gtgtgtgcag gttttctttg tccgtcaggg
     9181 taagctcatc gagcgcgatg tcaatctctt cccctacttc aatgatccag atgaggattt
     9241 tttgacctat gtaggacaat tctatcaaga aaaatcccat ctagttccca atgaggtact
     9301 gattccgcaa gatattgacg aagaggctgt caaggccttg gtggattcca agattcttaa
     9361 gcctcaacgt ggagagaaaa agcagctggt caatttagct ataaaaaatg cccgtgttag
     9421 cctagagcag aagttcaacc tgctagagaa atctgtcgag aagacccaag gagctattga
     9481 aaatctagga cgcttgcttc aaatcccaac cccagttcgc atcgagtcct tcgataactc
     9541 caatatcatg ggaactagtc ctgtttcagc catggtggtc tttgtcaacg gcaagcctag
     9601 taagaaggat taccgtaagt ataaaatcaa gaccgtagtc gggccagacg actatgccag
     9661 tatgcgagag gtcattcgca gacgctatgg tcgagtacag cgtgaggctt tgactcctcc
     9721 agatttgatt gtgattgatg gggggcaagg tcaagtcaat attgctaagc aagtcatcca
     9781 agaagaactg ggcttggata ttccaattgc aggtctgcaa aagaatgata agcaccaaac
     9841 ccatgaattg ctctttggag atccgctgga agtggtggac ttgtctcgca attctcagga
     9901 atttttcctc ctccaacgca ttcaagatga ggtccaccgc tttgccatta ccttccaccg
     9961 ccaactgcgc tccaaaaatt ctttctcatc tcaattggat gggattgatg gtctgggacc
    10021 taaacgcaag caaaatctca tgaggcactt caagtctctg accaaaatca aggaagccag
    10081 tgtggatgag attgtcgaag ttggagtacc aagagcagta gcagaggctg tgcaaaccaa
    10141 gttgaacccg caggaaacag aaatcttgct tcaagtggcg gaagaaagag tagattacca
    10201 aacggaagga aaccacaata aaccataaaa tcgcaatttt atcagatgtt catggcaatg
    10261 cgacggcgct agaagcagtg attgcagatg ctaaaactca aggagccagt gaatattggc
    10321 tcatgggaga cattttcctc cctggtccag gtgcaaatga cttggtcgct ctgttaaagg
    10381 aacttcctat cacggcaagt gttcgaggca attgggatga ttgtgtcctt gaggctttag
    10441 atgggcaata tggcttagaa gacccacagg aagttcagct cttgcgtatg acacagtatt
    10501 tgatggagcg aatggatcct gcaacgattg tctggctacg aagcttgcct ttgctggaaa
    10561 agaaagaaat tgacggattg cgcttttcta tctctcataa tttacctgac aaaaactatg
    10621 gtggtgactt gctagttgag aatgatacag agaaatttga ccaactgcta gatgcggaaa
    10681 cggacgtggc agtttatggt catgttcaca agtagttgct tcgttatggt agtcaagggc
    10741 aacaaatcat caatccaggg tcgattggca tgccctattt taattgggag gcgttaaaaa
    10801 atcaccgtgc ccagtatgcc gtgatagagg ttgaagatgg ggaattggta aatatcctat
    10861 ttcgtaaagt cgcttatgat tacgaggctg agttagaatt tgccaaatcc aagggttttc
    10921 cctttatcga aatgtatgaa gaactacgtc gtgaagataa ctatcagagg cacaatctgg
    10981 aactattagc aagcttaata gaaaagcata ggtatgtaga ggatgtgaag aatttttttg
    11041 attttttgta agagtttcct aaaataacca atgcaaacta aaaaagcgat tggctgatcc
    11101 catcgctttt aatatatctt atactcaata tgaaaatcaa agagcaaact aggaagctag
    11161 ccgtaggttg ctcaaagcac agctttgagg ttgcagataa agctgacgtg gtttgaagag
    11221 attttcgaag agtgttattg taactgagat tgatctggga ggtaagaacc acctagatag
    11281 gtattgctga gtttttcaag ggttccgtct tgatagagtt ctttgagcgc tttatcaaat
    11341 tgctctttaa actctttttg gtcgcttgag aaaatgatat aattgctggg gctatctgca
    11401 gaaggtaaat caacgactga gaggtctaaa ccacggtcct tgataatctt ttgaacggat
    11461 accttgtcaa aaactaggaa atcaaactct ccgttagcaa ggtctaggat tcgtttacca
    11521 atatcctcac cagaaaaatt aattgtagcg ggattatcag tgtgtttctg attccagtta
    11581 ttgatgaatt gagcgttaga agttccggta tcctcttgtg ttgttttacc agcgatctgg
    11641 tcaagagaag tcaaaggatt tttcttgttg ctgacaagga cgaggggatt gttggaaatc
    11701 ggaagcgagt aaaggtattt ttcagcacgc tcttttgtgt aactcaaatt attggccgca
    11761 gcctgatagt gaccagaatc aagtcctggg aagatacttt cccaggcggt tctttgaaat
    11821 tgaatctcgt agtcgctgag tttttcatct actgccttta gaacttcgat atcgaagcct
    11881 gtcagattgc ctttatcttc gtagtcaaat ggtggcacgt cgccagctgt agcaaggacg
    11941 attgtctttt gagcgctagt ctctttgggt gtagcttgat tcccacaggc aaccaaaaat
    12001 ggtaggatag ctagtaatag gctaaatttt ttcatactgt ctccattcaa atgtaaagta
    12061 cttgctagtt tatcagaaac tttcctgata aaccaatata tatcttttat gtctgtgata
    12121 gaaaaactta atcatgaaaa atccctgcaa gctctttcaa attatggtag aatggaagca
    12181 gtagaattag aaaaggaaat cgattatgaa atttcttgaa ttaaataaaa aacgtcatgc
    12241 gaccaagcat ttcactgata agcctgttga tccaaaagat gtgcgtacgg ctatcgaaat
    12301 tgcaaccttg gcgccaagcg cccacaacag ccagccttgg aaatttgtag ttgttcgtga
    12361 gaaaaatgct gaactggcaa agttggctta tggttcaaac tttgagcagg tatcatcagc
    12421 acctgtaacc attgccctat ttacagatac agaccttgcc aaacgcgctc gtaagattgc
    12481 ccgagttggt ggtgctaaca atttctcaga agaacaactt caatacttca tgaaaaattt
    12541 gccagctgaa tttgcacgct acaatgaaca acaagtcagc gactacctag ctctcaatgc
    12601 aggtttggtt gccatgaact tggttcttgc attgacagac caaggaattg gttctaacat
    12661 tattcttggt tttgacaaat caaaagttaa tgaagttttg gaaatcgaag accgtttccg
    12721 cccagaactc ttgatcacag tgggttatac agacgaaaaa ttggaaccaa gctaccgctt
    12781 gccagtagat gaaatcatcg agaaaagata gaaagaagaa aaaatgacag caattgattt
    12841 tacagcagaa gtagaaaaac gcaaagaaga cctcttggct gacttgttta gccttttgga
    12901 aatcaattca gaacgtgatg acagcaaggc tgatgcccag catccatttg ggcctggtcc
    12961 agtaaaagcc ttggagaaat tccttgaaat cgcagaccgc gatggctacc caactaagaa
    13021 tgttgataac tatgcaggac attttgagtt tggtgatgga gaagaagttc tcggaatctt
    13081 tgcccatatg gatgtggtgc ctgctggtag cggttgggac acagaccctt acacaccaac
    13141 tatcaaagat ggtcgccttt atgcgcgcgg ggcttcggac gataagggtc ctacaacagc
    13201 ttgttactat ggtttgaaaa tcatcaaaga attgggtctt ccaacttcta agaaagttcg
    13261 cttcatcgtt ggaacagacg aagaatcagg ctgggcagac atggactact actttgagca
    13321 cgtaggactt gccaaaccag atttcggttt ctcaccagat gctgaatttc caatcatcaa
    13381 tggtgaaaaa ggaaatatca cggaatacct ccactttgca ggagaaaata caggtgttgc
    13441 ccgtcttcac agctttacag gtggtttacg tgaaaatatg gtaccagaat cagcaacagc
    13501 agtcgtttca ggtgacttgg ctgacttgca agctaaacta gatgcctttg ttgcagaaca
    13561 caaacttaga ggagaactcc aagaagaagc tggcaaatac aaggtgacga tcattggtaa
    13621 atcagcccac ggtgctatgc ctgcttcagg tgtcaatggc gcaacttacc ttgccctctt
    13681 cctcagtcag tttggctttg ctggtccagc caaagactac cttgacatcg caggtaaaat
    13741 tctcttgaac gatcatgagg gtgaaaatct taagattgct catgtggatg aaaagatggg
    13801 tgctctttct atgaatgccg gcgtcttccg cttcgatgaa acaagtgctg ataataccat
    13861 tgccctcaac atccgctatc caaaaggaac aagtccagaa caaatcaagt caatccttga
    13921 aaacttgcca gttgtttctg ttagcctgtc tgaacacggt cacacgcctc actatgtgcc
    13981 aatggaagat ccacttgtgc aaaccttgtt gaatatctat gaaaaacaaa ctggctttaa
    14041 aggtcatgaa caagtcatcg gtggtggaac ctttggtcgc ttgttagagc gcggagttgc
    14101 ctatggtgct atgttcccag actcaattga taccatgcac caagccaatg aatttatcgc
    14161 cttggacgat ctcttccgag cagcagcaat ctatgccgaa gctatttacg aattgatcaa
    14221 ataaaacgat agaagtctga gatcttatgc ttggacttct ttttggaggg aaagtagatg
    14281 tctcaaatcg aaagaatcaa acaggctatc atggcggatt cacagaatgc cagctataca
    14341 gagcgtggca ttgagcctct ctttgcagcg ccaaaaactg ctcgcatcaa tatcatcggt
    14401 caggctccgg gacttaaaac tcaagaagca ggcctttact ggaaagataa aagtggtgac
    14461 cgcttgcggg actggctagg tgtggatgaa gatacctttt acaattcagg ttattttgct
    14521 gttttgccta tggatttcta ctttccagga catggcaagt cgggtgatct tccgcctcgt
    14581 acaggttttg cagaaaaatg gcatccgcag ctcttacagg aattgcctga tattcagtta
    14641 accctcttga ttgggcaata tgcccaagcc tactatttac aggagaaaat cagtgggaag
    14701 gtaacggaga gggtgaaaca ctataaagac tatctgccag cctattttcc gctagttcac
    14761 ccatcaccac gaaatcaaat ctggatggcc aaaaatcctt ggtttgaggc agaagtagtg
    14821 ccagatttga aaaaaagaat taaaaccatt ttatagtcaa tgaaaatcaa agagcaaact
    14881 aggaagctag tcgtaggctg ctcaaagtac agctttgagg ttgcagataa aactgacgaa
    14941 gtcggtaaca tacgcacggt aaggcgacgc tgacgtggtt tgaagagatt ttcgaagagt
    15001 attagaagaa aaagaatgaa agaaatagcc tttgacgcat tttaccagct ttaccaaaac
    15061 gaccagcttt ctctagtgga tgtgagagaa gtggatgagt ttgcagctct tcatttagaa
    15121 tgtgcccaca acctaccgct tagtcaattg gctgatagtt atgattaatt ggacaaagat
    15181 cgcttgcatt atattatttg caaatctgga atgagatcgg cgcgtgcttg ccaattcctg
    15241 ttagaacaag gttataatgt tatcaatgtc cagggtggca tgttagcctt tgaagaactt
    15301 taaaattttg catttctcct acttggtgtg gactgggtag gagagtttta tttttagata
    15361 attcttattt ttaagaaaat tgaaaacatt taatatttgc ctcgtgatgc ttttttcaga
    15421 ctcctaatcg tggtatacta ggtcagtatt ttataaatat gaaggagatt tttatggcta
    15481 aaaaaggtac cctaacaggt ttgctcctgt ttggaatatt ttttggtgcg gggaacttga
    15541 tttttccgcc ttctctaggt gctctatctg gagaacattt tcttcctgcc atcgcaggtt
    15601 ttgtcttttc aggcgttggt atcgccgtct tgacccttat tattggaacg ctaaatccta
    15661 aaggatatat ctacgagatt tcaacgaaga tagcgccttg gtttgcgact ctttacctct
    15721 cagttcttta cttgtcaatc ggtccattct ttgctacccc acgtactgct acaacagctt
    15781 acgaagtagg gattagcccc cttttgtcgg atgcaaataa aggacttggc ttgattgtat
    15841 ttacggttct gtattttgcg gcagcctatt tgatttcgct taatccatca aaaatcttag
    15901 accgcattgg acgtgtttta acgccagtct ttgcaatttt gattgttatc ttggtcgttc
    15961 tgggagctat caaatatggt ggaacaagtc ctcaagctgc ttcagctgct tatcaagctt
    16021 ctgcctttgg tacaggtttc ctagaaggtt acaatacctt ggacgccctt gcttcagtgg
    16081 cctttagcgt aatcgcagtt caaaccttga aacaacttgg attttcaagt aagaaagaat
    16141 acatttcaac tatttgggtt gttggtatcg ttgttgccct tgccttcagc gctctttaca
    16201 tcggtttagg ttttcttgga aatcatttcc cagtaccagc tgaagcgatg aagggtggaa
    16261 caccaggtgt ttacatcttg tcacaagcca ctcaagaaat ctttggctca acagctcaac
    16321 tcttccttgc agctatggtt accgtaacct gcttcacaac gactgttggt ttgattgtgt
    16381 caacagctga gttctttaat gagcgcttcc cacaaatcag ctacaaggtt tatgcgacag
    16441 cctttacctt gattggattt gctattgcca atttgggtct tgatgcgatt atcaagtact
    16501 caattccagt actggttatc ttgtacccaa tcacgattgc tatcgttatg attgtcattg
    16561 tcaacaaatt tgttgccctt tcaaaaccag gtatgcagtt gacaattgct gtggttacag
    16621 ttattgccat tgcaagcgta ctaggaagct cgtttaaggt tgagtttctt gcaaatcttg
    16681 ttagcgttct tccttttgcc aaggcatctc tcccatggtt ggtgccagcc attgttggaa
    16741 tcttgctctc attggttcta ccaaacaagc aagaaagcga tgtttttgaa atggaataat
    16801 cacttaaatc acttttgtag ccaagtctac aggagtgatt ttcttttttt atccgatgat
    16861 aaatgtgtta taataggtag cgaaagaggt gaagaaatga atcaaacagt agaatatatc
    16921 aaagaactga cagccatcgc atcgccaacg ggcttcactc gtgaaatttc gaattattta
    16981 gtcaagactc tagaaggttt tggttaccag ccagttcgca cagctaaggg tggtgtcaat
    17041 gtgaccatca aaggtcaaaa tgatgagcaa catcgctatg tgactgccca tgtggatacg
    17101 ctgggtgcta ttgttcgtgc tgtcaaacca gatggtcgtc ttaaattgga ccgtatcggt
    17161 ggttttcctt ggaacatgat tgaaggtgaa aactgtaccg ttcatgtggc tagcacaggt
    17221 caaaaggtat caggaaccat cctcatccac caaacttctt gccatgtcta caaggatgca
    17281 ggaactgcag aacgcacgca ggacaatatg gaagtgcgtt tggatgtaaa agtaagcaat
    17341 gaaaaagaaa ctcgtgctct tgggattgag gtcggtgatt ttatcagctt cgatacacga
    17401 actgttgtga ctgatacagg ttttatcaag tctcgtcatt tggatgataa ggtcagcgca
    17461 gcgattttgc tcaacctgct tcgtatttat aagaaagaga agattgaatt gccagtaaca
    17521 actcattttg ctttttcagt ttttgaagaa gtaggacatg gtgctaattc taacattcct
    17581 gctcaggtag tagaatatct ggctgtggat atgggagcta tgggagatga ccagcagaca
    17641 gatgagtaca cagtatctat ctgtgtcaag gatgcttcag gtccttatca ctatgacttc
    17701 cgtcaacact tggttgcctt ggctaaagag caagatattc catttaagct cgacatctat
    17761 ccattttacg gatcggacgc ttcagcagcg atgtcagcag gtgcagaggt caagcatgca
    17821 cttcttggag ctggtattga gtccagtcac tcttatgagc gcacccacat cgattcagta
    17881 atagcgactg agcgtatggt tgatgcttat cttaagagcg aggcggtatc atagaatgtg
    17941 cttaatctgt cagagaatcg aactcattaa agctgggcaa aatccctact ttgtaaaaga
    18001 actagaaaca ggctatgttg ttattggtga ctaccaatac tttaagggct ataccttatt
    18061 tttagcaaaa gatcatgtca cagaactcca tcatatggaa acttctgtaa aacttcgctt
    18121 tttagaggaa atgagtttag tccaagaagc agtcgccaaa gcatttgagg ctgaaaagat
    18181 gaatattgaa ctcctaggaa atggcgatgc ccacgctcat tggcatcttt ttccccgaag
    18241 agcaggtgat atgaagagtc acggtttgaa tggccgtggc ccagtctggt gggtgccctg
    18301 ggaagaaatg gcggcagaag attgccaagt acaatcgcca gagctggaag agatgattaa
    18361 aatcttatct catgaattgg agaagtattt ggcctaagag agaggaagaa agaaatgaaa
    18421 aaaagatatg tcattttatc tggtttacta gcattgaccc ttgcagcttg ttcgcaagaa
    18481 aaaaccaaag ttgaagaaaa cactcaaaaa acggagcaaa gcagtcaacc ggaaggaact
    18541 gtaggtagca aatctcaagc ttctagtcag aaaaaggcag aagttagcaa taaagggagc
    18601 tattatagta tccaagggaa atacgatgag attatcctag ctaataaacg ttatcctttg
    18661 tcaaaagatt acaatccagg agaaaatcca acagccaagg cagagttgct caaactaatc
    18721 gcagctatgc aggctgaggg ctatccaatc agtgaccaat acagtgggtt taggagttat
    18781 gaaactcagg ctaagctcta ccaagactat gtcaatcaag atggtaagga agcagcagat
    18841 cgctactcag cacgcccagg ttacagtgaa caccagacgg gtcttgcttt tgatttgatt
    18901 gggacagatg gggatttagt tactgaagaa aaggcagccc agtggctctt ggaccatgcg
    18961 gctgactatg gctttgttgt tcgctatctt aaaggcaagg aaaaggagac tggctacatg
    19021 gcagaagaat ggcatctccg ctacgttgga aaagaagcca aagaaattgc tgcaagtggt
    19081 ctcagtttgg aagagtacta tggctttgaa ggcggcgatt atgtcgatta aaaatgaaag
    19141 ttttctcttg catttttaga taaatagtgt ataatggaaa ggtatgtgta aagcatactt
    19201 gtgggaggta aaaatctcta attaccgcca aaaccacaaa ggaggattta aaaatggcta
    19261 aaaaagtcga aaaacttgta aaattgcaaa tccctgctgg taaagctaca ccagctccac
    19321 cggttggacc tgctcttggt caagctggta tcaacatcat gggattcaca aaagagttca
    19381 acgctcgtac agctgaccaa gctggtatga tcattccagt tgttatctca gtttacgaag
    19441 ataaatcatt tactttcgtt acgaaaacac caccagctgc tgttcttttg aaaaaagctg
    19501 caggtgttga aaaaggatca ggtacaccta ataaaactaa agttgctaca gttactcgtg
    19561 cacaagtaca agaaattgca gaaactaaga tgccagattt gaacgcagca aacgtagagt
    19621 ctgcaatgcg tatgatcgaa ggtactgctc gttctatggg attcactgtt gttgactaat
    19681 caataacaca gtagaaaatc tcacagcagt ctattagttg cttttcatac taggcaagtg
    19741 actgaggctt gtacttggat acagcaaggg agcttaaggc cgtaggaaga gaaaaatagt
    19801 agactgaaaa cccgcaagac ttcatcattt cgagaagtga cgtgggagat gaaaatcgat
    19861 tgaaccactt acaaggagaa tagaaaatgg ctaaaaaaag caaacaactt cgtgctgctc
    19921 ttgagaaaat cgacagcaca aaagcataca gtgtagaaga agctgtagca cttgcaaaag
    19981 aaactaactt tgcaaaattt gatgcaactg tagaagttgc ttacaacttg aacatcgacg
    20041 ttaaaaaagc tgaccaacaa atccgtggag caatggtatt gccaaacggt actggtaaaa
    20101 cttcacgtgt tcttgttttc gcacgtggtg caaaagctga agaagcaaaa gctgctggtg
    20161 cagactttgt tggtgaagat gaccttgttg ctaaaatcaa cgacggttgg ttggacttcg
    20221 acgtagttat cgctacacct gatatgatgg ctcttgttgg acgtcttgga cgtgtccttg
    20281 gaccacgtaa cttgatgcca aaccctaaaa ctggtactgt aacaatggat gttgctaaag
    20341 cagttgaaga gtctaaaggt ggtaaaatca cttaccgtgc tgaccgtgca ggtaacgttc
    20401 aagcaatcat cggtaaagta tcatttgaag ctgaaaaatt ggttgaaaac tttaaagctt
    20461 tcaacgaaac aatccaaaaa gtaaaaccag ctacagctaa aggaacttac gtaacaaact
    20521 tgactatcac aactactcaa ggtgttggta tcaaagttga cgtaaactca ctttaatcag
    20581 tagttaaagt aatgtaaaaa agttgaagac actatgtctc aacttttttt gatgtacgac
    20641 gggcatgtcg tatatctgag gtgtaagtcc tcggtgggca cctactaccg gtgaacccaa
    20701 tagcgattcc caagtctgac gctggaaata agaatcgtca gaggaaggga tagcgaaatc
    20761 gtggctctac gaacaggaac gtgatagtaa ggcctatata gcggataagg tatctggctg
    20821 gggcgaccac tatcaattat aataaaatga aataagaata ggacgaatcg ttcaggacag
    20881 tcaaatcgat ttctaacaat gttttagaag tagaggtgta ctattctagt ttcaatctac
    20941 tatagtagct cagaagtcgg tacttaaacg tgctatatca aaaccagtcc ttgaaaaacg
    21001 tggactggtt tcgtgtttgg attattacct tgaacgacat gcgttaaaag ttagttgaac
    21061 cgccgtatgc cgaacggcac gtacggtggt gtgagagggg ctagagatta tcccctactc
    21121 gatttcgaaa tctagtggaa tgaatctgga atagtccatc gagctttcta atactctttg
    21181 aaaatctctt caaaccacgt caacgtcgcc ttgccgtgcg tatggttact gacttcgtca
    21241 gttctatcca caacctcaaa acagtgtttt gagcaacctg cggctagttt cctagtttgc
    21301 tctttggttt tcattgagta taacacattg ttagaagttg gtttaaattt cctaatcagt
    21361 ttgttcacat ttaccttcga tatattatat cccatagtta aggttggtca tacagatgat
    21421 tatagccatg gagccgtaaa acttagtgtt tctttagttg acaaagatgc catgaaaaaa
    21481 atatctgtaa ctgtaatag
//
LOCUS       DAVMGZ010000020        21465 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000020 DAVMGZ010000000
VERSION     DAVMGZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21465
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.021"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            43..264
                     /locus_tag="VYQ93_000633"
     CDS             43..264
                     /locus_tag="VYQ93_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3335367.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            781..1034
                     /locus_tag="VYQ93_000634"
                     /pseudo
     CDS             781..1034
                     /locus_tag="VYQ93_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1162..2391
                     /locus_tag="VYQ93_000635"
     CDS             1162..2391
                     /locus_tag="VYQ93_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV3335368.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFAGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2411..5107
                     /gene="ppc"
                     /locus_tag="VYQ93_000636"
     CDS             2411..5107
                     /gene="ppc"
                     /locus_tag="VYQ93_000636"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV3335369.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5190..5407
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5520..6524
                     /locus_tag="VYQ93_000637"
     CDS             5520..6524
                     /locus_tag="VYQ93_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3335370.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6593..7459
                     /locus_tag="VYQ93_000638"
     CDS             6593..7459
                     /locus_tag="VYQ93_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3335371.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7456..8214
                     /locus_tag="VYQ93_000639"
     CDS             7456..8214
                     /locus_tag="VYQ93_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3335372.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDAPKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8304..10076
                     /gene="dnaG"
                     /locus_tag="VYQ93_000640"
     CDS             8304..10076
                     /gene="dnaG"
                     /locus_tag="VYQ93_000640"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV3335373.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     MPITKQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLP
                     PEVLAEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEA
                     SHVGDTDTALEELERLISQKRRME"
     gene            10079..11188
                     /gene="rpoD"
                     /locus_tag="VYQ93_000641"
     CDS             10079..11188
                     /gene="rpoD"
                     /locus_tag="VYQ93_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV3335374.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11202..11531
                     /locus_tag="VYQ93_000642"
     CDS             11202..11531
                     /locus_tag="VYQ93_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV3335375.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11646..12689
                     /gene="cpoA"
                     /locus_tag="VYQ93_000643"
     CDS             11646..12689
                     /gene="cpoA"
                     /locus_tag="VYQ93_000643"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV3335376.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12705..14030
                     /locus_tag="VYQ93_000644"
     CDS             12705..14030
                     /locus_tag="VYQ93_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV3335377.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14061..14237
                     /locus_tag="VYQ93_000645"
     CDS             14061..14237
                     /locus_tag="VYQ93_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV3335378.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14296..14430
                     /locus_tag="VYQ93_000646"
     CDS             14296..14430
                     /locus_tag="VYQ93_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV3335379.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14494..15804
                     /gene="obgE"
                     /locus_tag="VYQ93_000647"
     CDS             14494..15804
                     /gene="obgE"
                     /locus_tag="VYQ93_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV3335380.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15814..15972
                     /locus_tag="VYQ93_000648"
     CDS             15814..15972
                     /locus_tag="VYQ93_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335381.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            17059..18318
                     /locus_tag="VYQ93_000649"
     CDS             17059..18318
                     /locus_tag="VYQ93_000649"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV3335382.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            18334..18885
                     /locus_tag="VYQ93_000650"
     CDS             18334..18885
                     /locus_tag="VYQ93_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3335383.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            18878..20155
                     /locus_tag="VYQ93_000651"
     CDS             18878..20155
                     /locus_tag="VYQ93_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV3335384.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFWVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20171..21031
                     /locus_tag="VYQ93_000652"
     CDS             20171..21031
                     /locus_tag="VYQ93_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV3335385.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            21256..21366
                     /locus_tag="VYQ93_000653"
                     /pseudo
     CDS             21256..21366
                     /locus_tag="VYQ93_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
BASE COUNT         6566 a         3688 c         4816 g         6395 t
ORIGIN      
        1 ttcttagttt tatatattat acttttctca ggagaaagct agatgtacag acgtttgaga
       61 gatttgaggg aagatcacga tttgacccaa aaacaaatag ctaaaatact ttcgtttaca
      121 aactcagctt atgctaaaat tgaacggggt gagcatacgt taacagcaga tgtattggta
      181 actctctcaa acttttacga tgtcagtaca gactacctat tggaattgac agattttcct
      241 gataaaattc gctttagaaa ataatctcct caatttcata gagtgtgaaa atgaggagat
      301 tttttatttg ttctttgaaa attaaatagt ccaaaatggt actttcctca tttgtggagc
      361 aaatttgaat ggctcgccat gataagagcg attttaaaac catcaataaa atagagcgat
      421 actttatatg ccatgataca aatgatatac aatgatactt ctgaccgttc aggctgccat
      481 cgtaaaagag cagcaagtga aattcttatg atgacttcat cagtcatgcc acgttaaatg
      541 tgtgagtttg ttagagaaac gcaattaatc ctcaaaaggt tccccgaacc ttttgagttc
      601 cacagacgca tcacgtggag tgtgtaagct tgttgctaaa agcgtaaaaa ccttggaacg
      661 aaaggaataa tagacttcct gcgaaacaaa aatacaatac aataaaacta tggatgatga
      721 agcaagtaaa caattaagcg atagccgttt caagagcctt gtaggtgttc agcgcacgag
      781 atgttagctg tgttaaaaac agcttatcaa cgcaacgtaa acgcgcaaaa ggtggacgaa
      841 aaaccaaatt aagcctagac gatctcctca tggtaactat tcaatacatg cgagaatagc
      901 gcacttatga acaaattgcg gctgattttg gcattcacga aagcaactta atccgtcgga
      961 gccaatgggt tgaagtaact cttgttcaaa atggttttac gatttcaaat tctgccctaa
     1021 ttatgaaaaa cagtaaaatt cgaaggattg taaggtaaga gtttttttct ttctgaaaaa
     1081 atggtataat agcaatcaaa actagaaaat aaaacggaat ttggaacaga tttgtctgta
     1141 tcctagtaga gtggtgatgc tatgaagatt agtaagaggc acttattaaa ttattccatc
     1201 ttgattccct acttgctttt atctattttg ggcttgattg tggtctattc gaccaccagt
     1261 gctattttaa ttgaagaagg caagagcgcc ttgcagttgg ttcgaaacca aggaatcttt
     1321 tggattgtta gtttgatact gattgcctta atttataaat tgagactaga ttttttgaga
     1381 aatgagcgac taatcatttt agttatatta atagaaatgc ttttattgtt cttggctcgt
     1441 tttattggta tttccgtaaa cggggcatac ggttggattt cggttgcagg agtaactatt
     1501 cagccagctg agtacttaaa aatcattatt atttggtatt tagctcaccg attctccaaa
     1561 cagcaagaag aaatagctac ttatgatttt caagttttga ctcaaaatca atggcttccc
     1621 cgtgctttta atgattggcg attcgttctc ctagttctga ttggaagttt gggaattttc
     1681 cctgatttag gaaatgcgac tattttagtc ttggtttcct tgattatgta tacagttagt
     1741 ggaatcgctt atcgctggtt ttcaaccatt ctggcgctcg tatctgccgc ttctgtcttt
     1801 gtcttgacca ctatcagcct aatcggtgtt gagacctttt caaaaattcc agtatttggc
     1861 tatgtagcca agcgctttag tgcctttttt aatccttttg ccgatcgtgc tgatgcaggt
     1921 caccagttag ctaattctta ttttgccatg gtcaatggtg gttggtttgg tctaggtctt
     1981 ggaaactcga ttgaaaaacg aggttatttg ccagaagctc atacagactt tgtcttttct
     2041 atcgtgattg aagaatttgg ctttgctggt gccagtctta ttttagctct cttgtttttc
     2101 atgattttgc ggattatctt ggtcggtatt cgagcggaga atcctttcaa tgccatggtt
     2161 gcactcggtg tcggagggat gatgttggtt caggtatttg tcaatatcgg agggatttcg
     2221 ggcttgattc catctacagg agtaaccttc cccttcttat cccagggtgg aaatagtctt
     2281 ctagtcttat cagtggcagt agcctttgtc ttaaatattg atgccagtga aaaacgcgct
     2341 aagttgtacc gagaattgga aaatcaacca atgaaccttc tgttgaagta ggataaagaa
     2401 aggatagttt atgtctcttc aaaaattaga aaattatagt aataaaagtg ttgtgcaaga
     2461 agaagtcttg attctaacag aattactgga agatattact aaaaatatgc ttgccccaga
     2521 gacctttgaa aaaataatac agttgaaaga attatcaacg caggaagatt atcaaggtct
     2581 aaaccgtcta gtgactagct tatcaaatga tgaaatggtc tatatttcac gctatttctc
     2641 tatcttacct cttttgatca atatttcaga ggatgtggat ttagcttatg aaatcaatca
     2701 tcaaaataat attgatcagg actatttagg taaattatct acaacgatta aattggtagc
     2761 agaaaaggaa aatgccgttg agatcctaga acacttgaat gttgtccctg ttttgacagc
     2821 ccatccaaca caagtgcaac gcaaaagtat gttggattta acaaatcata ttcatagtct
     2881 tttgcgtaaa taccgtgatg ttaagttggg gttgatcaat aaagataaat ggtacaatga
     2941 tttgcgtcgt tacatcgaaa ttatcatgca gacagacatg attcgtgaga aaaaattaaa
     3001 agtgactaac gaaatcacga atgctatgga atattataac agctcctttt tgaaagctgt
     3061 acctcatttg acgacggagt ataagcgctt agcgcaagcg catggtctga atttaaaaca
     3121 ggctaaacca atcaccatgg gtatgtggat aggtggtgac cgtgatggaa atccatttgt
     3181 tacagcagag accttgaagc agtctgcact cactcaatgt gaagtcatca tgaactacta
     3241 tgataaaaag atttaccaac tttatcgtga attttctctt tcaactagca ttgtcaacgt
     3301 cagcaagcaa gtcagagaaa tggctcgtca atccaaggat aactcgattt accgcgaaaa
     3361 agagctttac cgtcgtgcct tgtttgatat tcaatcaaaa attcaggcaa ctaaaaccta
     3421 tctgattgag gatgaagaag ttgggactcg ttatgaaacc gccaatgatt tctacaagga
     3481 tttgattgcc attcgagatt ctctactaga aaataagggt gagtccttga tttcaggtga
     3541 ttttgtggaa ttattgcagg cagtagagat atttggtttt tacctagcat caattgatat
     3601 gcgacaagac tctagcgtct atgaagcctg tgtggcagaa ctcttgaaat cagcaggaat
     3661 tcattctcgt tatagcgagt tgagcgaaga agaaaagtgt gaccttctct tgaaagaatt
     3721 agaagaagat ccccgaattc tttctgcgac tcacgcagaa aaatcagaat tattagcaaa
     3781 agaattagct atttttaaga cggctcgtgt tttgaaagat aagttgggag atgatgtcat
     3841 ccgtcagacc atcatttcac atgcaaccag cctttctgat atgctagaat tagctattct
     3901 gttaaaagaa gtaggactgg tggatacgga aagggcgcgt gttcagattg ttcccctttt
     3961 tgaaacaatt gaagacttgg atcattcaga agaaacaatg agaaaatatc tttctcttag
     4021 ccttgccaaa aaatggattg actcacgaaa taactaccaa gaaatcatgc ttggctactc
     4081 tgacagtaat aaagatggcg gttacttgtc atcatgttgg accctctaca aggctcaaca
     4141 acaattgact gctattggag atgaatttgg cgttaaggtt accttcttcc atggtcgtgg
     4201 tggtactgtc ggtcgtggtg gtgggccaac ctatgaagcc attacatctc aaccgctcaa
     4261 gtctatcaag gatcgtatcc gcttgacgga gcagggtgaa gtaattggga ataaatacgg
     4321 taacaaagac gccgcttact ataaccttga aatgctagta tcggcagcta ttaaccgtat
     4381 gattactcag aagaagagcg ataccaatac cccaaatcgt tatgaagcca ttatggatca
     4441 agtagtggac cgtagttacg atatctaccg tgatttggtc tttggtaatg agcatttcta
     4501 tgattatttc ttcgagtcaa gtccaatcaa ggctatttca agttttaata ttggttctcg
     4561 tccagccgct cgtaagacta ttactgaaat cggtggtttg cgtgccatcc cttgggtatt
     4621 ctcatggtca cagagtcgtg ttatgttccc tggatggtac ggggttggtt caagcttcaa
     4681 ggaatttatc aataaaaatc cagagaatat tgctatctta cgagatatgt accaaaattg
     4741 gcctttcttc caatcgcttc tttcaaatgt tgatatggtt ttgtcaaaat caaatatgaa
     4801 tattgctttt gaatatgcta aactttgtga agacgagcaa gttaaggcca tctatgagac
     4861 tattttaaat gaatggcaag ttactaagaa cgttatcttg gctattgaag gacatgacga
     4921 actcttagct gacaatccat atctaaaagc tagtctggat taccgtatgc cttactttaa
     4981 tattctcaac tatattcagt tagagttgat taaacgccaa cgtcgtggag aattgtccag
     5041 tgatcaagaa cgattgattc atatcaccat caacggaatt gcgacaggat tgcgtaattc
     5101 aggttgataa ttttcaagag tgaatgctaa aagtgaatat caaaaaaatt ctaatagact
     5161 attgacaagt agtttaaaaa tgatataatt taaccattca gaaaagtaat catacaaact
     5221 ttttagagag tctgtggtag ctgaaaacag ataagtggcg atgatgaaaa ttgggctgaa
     5281 tgctatttag aatttgaaat tataaaaatt cggtaagcac accttacagt gcatctcgtt
     5341 attgcgagac tgagcgatag ggaaattccc tataattgag gtggtaccgc gcatcgacgt
     5401 cctcacacaa gttttttgtg tgaggatttt tttgatggag gttagtatgg aaagaaaacg
     5461 atggcgtcgc ttgtttagat aagtgaaata tgttaaagga aataaaaagg agaaacagaa
     5521 tgaaaaataa acgtttaatt ggaattattg ctgcattagc agtcttagta gcaggaagct
     5581 tgatttattc ttcaatgaat aaatcagaag ctcagaataa taaggatgag aagaaaataa
     5641 ctaagattgg tgtgcttcaa tttgtgagcc atccatccct tgatttgatt tataaaggga
     5701 tccaagatgg acttgcagaa gaaggatata aagatgatca agttaaaatt gattttatga
     5761 actcagaagg tgaccaaagt aaggttgcga caatgagtaa acaattggtt gcaaatggga
     5821 atgaccttgt ggttggtatc gcaacaccag cagcccaagg gttggctagt gcaacaaaag
     5881 acctaccggt tatcatggcc gctattacag acccaattgg tgctaacttg gttaaagatt
     5941 tgaaaaaacc aggtggcaac attacagggg tatctgacca caatccagct caacaacaag
     6001 ttgaactcat caaggctctg acaccgaatg tgaaaacaat cggagctctt tactcaagta
     6061 gcgaagacaa ttcaaaaaca caggtcgaag aatttaaggc ttatgctgaa aaagcaggtc
     6121 tgacagtgga aacatttgca gttccttcaa caaatgaaat tgcctcaact gtcactgtta
     6181 tgactagcaa ggtagatgct atttgggttc caattgataa caccattgca tcaggatttc
     6241 caacggttgt ctctagcaat caaagttcta agaaaccaat ttatcccagt gcgacagcta
     6301 tggtagaagt aggtggtttg gcatcagttg taattgacca acatgacctt ggtgtggcaa
     6361 caggtaaaat gattgtgcaa gtcttgaaag gtgcaaaacc agccgatacc ccagtcaatg
     6421 tcttttcaac tggtaagtca gtcatcaata aaaaaatagc acaagaacta ggtattacta
     6481 ttcctgagtc tgttctcaaa gaagcaggac aagtcatcga ataaataagg tagggaggag
     6541 gagactcttc ccatttttta aaaagatgaa tatagaaagg attaaataaa atatgattgt
     6601 ttccattatt tctcaaggat ttgtctgggc tattctaggt ctgggaatct ttatgacatt
     6661 taggatttta aactttccag atatgacgac agaaggttcc ttccctcttg ggggagctgt
     6721 tgctgtcact ttgataacca aaggcgtgaa cccattttta gcgacacttg ttgctgtagg
     6781 agcaggttgt ttggctggaa tggcagcagg ccttctttat acaaaaggga agatcccaac
     6841 cttgctctca gggattttgg tgatgacttc ttgtcactca atcatgctct tgattatggg
     6901 acgtgcgaat ttaggcctgc ttggaaccaa gcaaattcag gatgttttgc cttttgattc
     6961 ggatttgaat caactcttga caggtctcat ctttgtgagt attgttattg ctctcatgct
     7021 ctttttcttg gacactaaac tcggacaagc ctatattgct acaggggata atcctgatat
     7081 ggctagaagt ttcgggattc atactggacg catggagctc atgggcttgg tcttatcaaa
     7141 tggtgtgatt gcccttgcag gtgccctcat tgctcagcaa gaaggttatg ccgatgtgtc
     7201 tcgagggatc ggggttatcg ttgtggggct tgcaagtttg attattggag aagttatttt
     7261 caagagtttg agcttggcag agcgtttggt tactatcgtt gtaggttcta tcgcttatca
     7321 atttttagtg tgggcagtta tcgcacttgg ctttaataca agttaccttc gtttatacag
     7381 tgccttgatt ttagcagtct gcctcatgat tccaacattt aagcaaacaa tcttgaaagg
     7441 agccaagtta agcaaatgac agcaattgta gaattaaaaa atgcaaccaa aatcgttaaa
     7501 aatggctttg atgaagaaaa gattatttta aatgatgttt ccttagaaat ttttgaacgg
     7561 gactttatca cgattttggg cggaaatggt gctggaaaat caaccctctt taacactata
     7621 gcagggacct tatcactaac tagtggaact atccgtattt taggtgaaga actcactaag
     7681 ttttcacccg agaagcgtgc caagtacctg tctcgtgtct tccaagatcc aaagatgggg
     7741 acagctcccc gtatgacggt cgctgaaaat cttttaatcg ccaagtttcg tggtgaaaag
     7801 cgtggattgt taccacgacg cttgactagc tataaggatg aatttcaggc aaccattgaa
     7861 aaagtaggaa atggtcttga gaaacacttg aatacaccga ttgagttctt atcaggtgga
     7921 caaagacagg ctttgagtct cttgatggca accttgaagc gacctgaatt actcctgtta
     7981 gatgagcata ctgctgccct ggatccaaag actagtgttg ctttgatgga attgacagat
     8041 gaatttgtta agaaagatca gctaacagcc cttatgatta ctcatcatat ggaagatgct
     8101 cccaaatacg gcaatcgctt gattgtcatg aaagaaggac ggattatcca agatttaaac
     8161 caagaagaaa aagcaaaaat gaaaatctct gattattatc aactctttga ataaagtagg
     8221 gaaaatgagt gaaatggttt actttttttc tgaaataaag tatactatat aaagtaaact
     8281 atgataacat ggaggtattg tgtatggttg acaaacaagt cattgaagaa atcaaaaaca
     8341 atgccaacat tgtggaagtc ataggagatg tgatttcttt acaaaaggca ggacggaact
     8401 atctagggct ctgtcctttt catggtgaaa aaacaccttc tttcaacgtt gtagaggata
     8461 agcagtttta tcactgtttc ggttgtggtc gctcaggtga tgtctttaag ttcatcgagg
     8521 agtaccaagg ggttcccttt atagaggcag tccaaatctt aggtcagcgt gttggaattg
     8581 aggttgaaaa accgctttat agtgagcaga agccagcctc acctcaccaa gctctttatg
     8641 atatgcacga agatgcagcc aaattttacc atgcgattct catgacaacg actatgggcg
     8701 aagaggccag aaattacctt tatcagcggg gtttgacaga tgaagtgctt aaacattttt
     8761 ggattggttt agcacctcca gaacgaaact atctctatca acgtttgtct gatcagtatc
     8821 gtgaagagga tttactggat tcaggcctgt tttatctctc ggatgccaat caatttgtag
     8881 acacctttca caatcgcatt atgtttcccc tgacaaatga ccaaggaaag gtcattgcct
     8941 tctcaggtcg tatctggcaa aaaacggatt cacaaacttc taagtataaa aacagccgat
     9001 cgactgcaat ttttaacaaa agttacgaat tatatcatat ggatagggca aaaaaatctt
     9061 ctggaaaagc tagtgagatt tacttgatgg aaggattcat ggatgttatt gcagcctatc
     9121 gggctggaat cgaaagtgct gtggcgtcga tgggaacggc cttgagtcga gagcatgttg
     9181 agcatctgaa aaggttaacc aagaaattgg ttcttgttta cgatggagat aaggctgggc
     9241 aagccgcgac attgaaagca ttggatgaaa ttggtgatat gcctgtgcaa atcgtcagca
     9301 tgcctgataa cttggatcct gatgagtatc tacaaaaaaa tggtccagaa gacttggcct
     9361 atctattaac gaaaactcgt attagtccga ttgagttcta cattcatcag tacaaacctg
     9421 aaaacagtga aaatctgcag gctcagattg agtttcttga aaaaatagct cccttgattg
     9481 ttcaagaaaa gtccatcgct gctcaaaaca gctatattca tattttagct gacagtctgg
     9541 cgtcctttga ttatacccag attgagcaga ttgttaatga gagtcgtcag gtgcaaaggc
     9601 agaatcgcat ggaaggaatt tccagaccga cgccaatcac catgccaatc accatgccaa
     9661 tcaccaagca gttatcggct attatgaggg cagaagccca tctactctat cggatgatgg
     9721 aatcccctct tgttttgaac gattaccgtt tgcgagaaga ctttgcattt gctacacctg
     9781 aatttcaggt cttatatgac ttgcttggcc agtatggaaa tcttcctcca gaagttttag
     9841 cagagcagac agaggaagtt gaaagagctt ggtaccaagt tttagctcag gatttgcctg
     9901 ctgaaatatc gccgcaggaa cttagtgaag tagagatgac tcgaaacaag gctctcttga
     9961 atcaggacaa tatgagaatc aaaaagaagg tgcaggaagc tagccatgta ggagatacag
    10021 atacagccct agaagaattg gaacgtttaa tttcccaaaa gagaagaatg gagtaataat
    10081 ggcaacaaaa caaaaagaag taacaacatt tgacgtacag gtagcagaat ttatccgtaa
    10141 tcataagcaa aaagggacag caacagatga tgaaatcaat gcaagtctgg ttattccttt
    10201 taccttggac gctgatggga ttgaagatct cttgcaacgg attcaggatg caggaatttc
    10261 tatcacagat aacgaaggaa atccaagtgc gcgtgttctt agcaatgaag aagaaccaga
    10321 actcagcgat gaggacttga ttgggtcaac ttctgctaag gtcaatgacc ctgtccgtat
    10381 gtacttgaaa gaaatagggg tcgttcctct cttgaccaat gaagaggaga aagagttggc
    10441 actggctgtt gaagctggtg atatcgaagc caaacaacgt cttgcggaag ccaatcttcg
    10501 tttggttgtt tccattgcca aacgctatgt cggtcgtggc atgcagttcc ttgacttgat
    10561 tcaagaagga aatatgggct tgatgaaggc ggttgacaag tttgactatt ctaaagggtt
    10621 caagttttca acttatgcaa cttggtggat tcgtcaggct atcactcgtg ctattgcgga
    10681 ccaagctcgt accatccgta tcccagttca catggttgaa actatcaata aattggttcg
    10741 tgaacagcgg aatctccttc aagaattggg gcaagatccg acaccagaac agattgctga
    10801 acgaatggat atgacacctg ataaggttcg tgaaatcttg aagattgccc aagaaccagt
    10861 atctcttgaa actcctatcg gtgaagagga cgatagccac cttggagact ttatcgaaga
    10921 tgaagtgatt gaaaatccag tggattatac gactcgtatc gtcttgcgtg agcaattgga
    10981 tgaaatctta gatactctta cagaccgtga agaaaatgtt ctgcgtctac gttttggact
    11041 agatgatgga aaaatgcgca cacttgaaga tgtggggaaa gtctttaacg taactcgtga
    11101 gcgtatccgt cagattgaag caaaggcttt gagaaaacta cgccaaccaa gtcgtagcaa
    11161 accgcttcgt gattttattg aagactaaga gtgaggaaaa tatggcttat acagaagagc
    11221 aaattgaaaa catcaaaaca cggattttaa cagccttgga agaagtcatc gaccctgagt
    11281 tgggaatcga tattgtcaat cttggtttga tctatgagat tcgttttgac ggtgacacag
    11341 ggcaaacaga gattgacatg actttgacaa ctatgggttg tcccttagca gaccttttga
    11401 cagaccagat ttatgatgct atgatagagg ttccagaagt aacggatact gaggttaaat
    11461 tagtctggta tccagcctgg acagttgaaa aaatgagtcg ctatgcccgc attgccctag
    11521 gcattaagta aacatataaa agcatgtgag agactgttgg aaaagcgagg aaatttcccc
    11581 tcttttcctc tagtctctcc tttcttttgc tgattttatt caaagaaaat gatataatag
    11641 tagttatgga gaaaaagaaa ttacgcatca atatgttgag ttcaagtgag aaagtagcag
    11701 gacagggagt ttcaggtgct taccgtgaat tagttcgtct tcttcaccgt gctgccaagg
    11761 accaattgat tgttacagaa aatcttccaa tcgaggcaga tgtgactcac tttcatacga
    11821 ttgattttcc ctattattta tcaaccttcc aaaagaaacg ctcagggaga aagattggct
    11881 atgtgcattt cttgccagct acacttgagg gaagtttgaa aattccattt ttcttaaagg
    11941 gaattgtgaa acgctatgta ttttcttttt acaaccggat ggagcacttg gttgtggtca
    12001 atcctatgtt tattgaggat ttggtagcag ctggtattcc acgtgaaaaa gtgacctata
    12061 ttcctaactt tgtcaacaag gaaaaatggc atcctctacc acaagaagag gtagtcagac
    12121 tgcgcacaga tcttggtctt agtgacaatc agtttatcgt agtaggtgct gggcaagttc
    12181 agaaacgtaa agggattgat gactttatcc gtctggctga ggaattgcct cagattacct
    12241 ttatctgggc tggtggcttc tcttttggtg gtatgacaga tggttatgaa cactataaga
    12301 aaattatgga aaatccccct aaaaatttga tttttccagg cattgtatcg ccagagcgga
    12361 tgcgcgaatt gtatgctcta gcggatcttt tcttgttgcc tagttacaat gagctctttc
    12421 ctatgactat tttagaagct gcgagttgtg aggctcctat tatgttgcgt gatttagatc
    12481 tctataaggt aattttggag ggaaattatc gggcgacagc gggtagagaa gagatgaaag
    12541 aggctatttt ggaatatcaa gcaaatcctg ctgccttaaa agatctcaaa gaaaaggcta
    12601 agaatatttc cagagagtat tctgaagagc atctgttaca aatctggttg gacttttatg
    12661 agaaacaagc cgctttaggg agaaagtaaa aagtgaggta atctatgcga attggtttat
    12721 ttacagatac ctattttcct caggtttctg gtgttgcgac cagtattcga accttgaaaa
    12781 cagaacttga aaagcaggga catgctgttt ttatctttac gacgacagat aaggatgtca
    12841 atcgctacga agattggcaa attatccgca ttccaagtgt tcctttcttt gcttttaagg
    12901 atcgtcgctt tgcctaccga ggttttagca aggcacttga aattgctaaa cagtatcagc
    12961 tagatattat ccatactcag acagaatttt ctcttggcct gttggggatt tggattgcgc
    13021 gtgaattgaa aattccagtc atccatacct atcacaccca gtatgaagac tatgtccatt
    13081 atattgctaa ggggatgttg atccggccga gtatggtcaa gtatctggtt agaggtttcc
    13141 tgcatgatgt ggatggggtt atttgtccta gtgagattgt ccgtgacttg ctatctgatt
    13201 ataaggtcaa ggttgaaaaa cgggtcattc ctactgggat tgaattagcc aagtttgagc
    13261 gtccggaaat caagcaggaa aatttgaaag aactgcgtag taaactaggg attcaagatg
    13321 gtgaaaagac gttgcttagt ctttcgagaa tctcctatga aaaaaatatt caagcagttt
    13381 tagcagcctt tgctgatgtt ctgaaagagg aagacaaggt taaactggta gtagctgggg
    13441 atggccctta tctgaatgac ctcaaagagc aagctcagaa cctagagatt caagactcag
    13501 tcatctttac agggatgatt gctcctagtg agacggctct ttactataaa gcggcggatt
    13561 tcttcatttc ggcatcgaca agcgaaacgc aaggtttgac ctacttggaa agcttagcca
    13621 gtggaacacc tgtcattgct cacggaaatc cttatttgaa caacctcatc agtgataaaa
    13681 tgtttggaac cttgtactat ggagaacatg atttggctgg tgctattttg gaagccctga
    13741 ttgcaacacc agacatgaac gagcatacct tatcagagaa attgtatgag atttcagctg
    13801 agaactttgg gaaacgagtg catgagtttt atctggatgc cattatttca aataacttcc
    13861 agaaagattt ggctaaagat gatacggtca gtcagcgtat ctttaagaca gttttgtatc
    13921 ttcagcaaca ggtggttgct gtacctgtaa aaggatctag acgcatgttg aaggcttcaa
    13981 aaacacagtt gatcagtatg agagactatt ggaaagacca tgaagaatag aaagaggaac
    14041 agctatgaaa aaaacaatta atgagaagcg gtcgtgataa aaagattgcg ggtgtttgtg
    14101 ctggggtggc ccattatctg gatatggatc cgactatcgt tcaagtcatt tggggtgttc
    14161 ttacttgctg ttacggagct ggaattgtag cttacattat tttatggatt atcgcaccag
    14221 tagcaactga ctattgaaaa ctagctcaat tcatgctaag ataatagaaa atagaatgta
    14281 acgaaggaga gaaaaatggc atttggagat aatggaaatc gtaaaaaaac tatgtttgag
    14341 aaaataacct tgtttatcgt gattatcatg ctagtagcaa gtttattggg aatttttgca
    14401 actgcaattg gtgccctcag taatctataa aatagattca agaaaattta gtgactggga
    14461 tttcccagcc cttttttaaa gtgagaagaa ataatgagta tgtttttaga tacagctaag
    14521 attaaggtca aggctggtaa tggtggcgat ggtatggttg cctttcgtcg tgaaaaatat
    14581 gtccctaatg gaggcccttg gggtggtgat ggtggtcgtg gaggcaatgt ggtcttcgtt
    14641 gtagacgaag gactacgtac cttgatggat ttccgctaca atcgtcattt caaggctgat
    14701 tctggtgaaa aagggatgac caaagggatg catggtcgtg gtgctgagga ccttagagtt
    14761 cgagtaccac aaggtacgac tgttcgtgat gcggagactg gcaaggtttt aacagatttg
    14821 attgaacatg ggcaagaatt tatcgttgcc cacggtggtc gtggtggacg tggaaatatt
    14881 cgtttcgcga caccaaaaaa tcctgcaccg gaaatctctg aaaatggaga accaggtcag
    14941 gaacgtgagt tacaattgga actaaaaatc ttggcagatg tcggtttagt aggattccca
    15001 tctgtaggga agtcaacact tttaagtgtt attacctcag ctaagcctaa aattggtgcc
    15061 taccacttta ccactattgt accaaattta ggtatggttc gcacccaatc aggtgaatcc
    15121 tttgcagtag ccgacttgcc aggtttgatt gaaggggcta gtcaaggtgt tggtttggga
    15181 actcagttcc tccgtcacat cgagcgtaca cgtgttatcc ttcacatcat tgatatgtca
    15241 gctagcgaag gccgtgatcc atatgaggat tacctagcta tcaataaaga gctggagtct
    15301 tacaatcttc gcctcatgga gcgtccacag attattgtag ctaataagat ggacatgcct
    15361 gagagtcagg aaaatcttga agaatttaag aaaaaattgt ctgaaaatta tgatgaattt
    15421 gaagagttac cagctatctt cccaatttct ggattgacca agcaaggtct ggcaacactt
    15481 ttagatgcta cagctgaatt gttagacaag acaccagaat ttttgctcta cgacgagtcc
    15541 gatatggaag aagaagctta ctatggattt gacgaagaag aaaaagcctt tgaaattagt
    15601 cgtgatgacg atgcgacatg ggtactttct ggtgaaaaac tcatgaaact ctttaatatg
    15661 accaactttg atcgtgatga atctgtcatg aaatttgccc gtcagcttcg tggtatgggg
    15721 gttgatgaag cccttcgtgc gcgtggagct aaagatgggg atttggtccg cattggtaaa
    15781 tttgagtttg aatttgtaga ctaggagact ggtatgggag ataaaccgat atctttccga
    15841 gatgcggatg gtaattttgt ttccgccgca gacgtttgga atgaaaagaa attggaagaa
    15901 ctatttaatc gtctcaatcc aaatcgtgcc ttgagattgg cacgaactaa aaaggaaaat
    15961 ccatctcagt aaagaagcta aaaaatcccg tgcctcatca gacacgggat tttgtggtac
    16021 gacaggcatg tatagcaaac tgaatctgga atagcacagc atatcttcta aaatattgct
    16081 ataaattgat ttgaatttca aaattaaatt gttttattct tatttcaatt tgttatagta
    16141 tatctgatgt caaagttctc ggcgagtcaa atagtgattc ccaagcctga ctatcgtgag
    16201 gtagcggatt aaaatggtct ggggatagac cgttttaagt ctgatgctgg aaataagaat
    16261 tgtcagaaga agggatagcg aaatcgtggc tctacgaaca ggaacgtgat aataaggcgt
    16321 atatagcgaa taagagggcc tcaaactcta aagtccaaaa aggtagtcgt aacctatatg
    16381 cgtaaatcat gagagtaatt gaattcgtac taagattttc tatttttact gtaacctttt
    16441 aacgtcctca tatcttgtat acacgaggaa agatgtacga cttatcccgt gaggtctatc
    16501 actataaaga gaaaacgaca gatagaagtg atcctgagtc acggttatct ttaaaataga
    16561 ttgtctgata ggatgctagc ctgaaaacgt cttgttcatg aaatatagga cggtatgtat
    16621 aaaacgcttc tgtgaactga gagaaggggg agaagttctt gctaaaattt agttgaacag
    16681 ccgtattccg atacttagat aagagatcta gtcttagctc ctactcagtt tcaggggata
    16741 aaaaaggggc aatagcgatt cgagaaagat tatactcttc gaaaatctct tcaaatcacg
    16801 tcaatatcgc cttgccgtat gtgtaggata ctaactacgt cagttccatc tacaatctca
    16861 aaacagtgtt ttgagcaacc tgcggctagt ttcctagttt gatctttgat ttttattgag
    16921 tattagtaat tcagttacta actcgtcaac tctgatttat ccaataaaat tgaaaaggat
    16981 ggaaaaaagg ataaatttat gatatacttt attttgaaga ccttattaga aatcttgaaa
    17041 gagtattgaa aacttagaat gagaaaaatt gttatcaatg gtggattacc actgcaaggt
    17101 gaaattacta ttagtggtgc taaaaatagt gttgtggcct taattccagc tattatattg
    17161 gctgatgatg tggtgacttt ggattgtgtt ccagatattt cggatgtagc cagtcttgtc
    17221 gaaatcatgg aattaatggg agctactgtt aagcgttatg acgatgtctt ggagattgat
    17281 ccaagaggtg ttcaaaatat tccaatgcct tatggtaaaa ttaacagtct tcgtgcatct
    17341 tactattttt atgggagcct cttaggccgt tttggtgaag cgacagttgg tctaccggga
    17401 ggatgtgatc ttggtcctcg tccgattgac ttacacctta aggcgtttga agctatgggt
    17461 gccactgcta gctacgaggg agataacatg aagttatctg ctaaagatac aggacttcat
    17521 ggtgcaagta tttacatgga tacggttagt gtgggagcaa cgattaatac gatgattgct
    17581 gcggttaaag caaatggtcg tactattatt gaaaatgcag cccgtgaacc tgagattatt
    17641 gatgtagcta ctctcttgaa taatatgggt gcccatatcc gtggggcagg aactaatatc
    17701 atcattattg atggtgttga aagattacat gggacacgtc atcaggtgat tccagaccgc
    17761 attgaagctg gaacatatat atctttagct gctgcagttg gtaaaggaat tcgtataaat
    17821 aatgttcttt acgaacacct ggaagggttt attgctaagt tggaagaaat gggagtgaga
    17881 atgactgtat ctgaagacag catttttgtc gaggaacagt ctaatttgaa agcaatcaat
    17941 attaagacag ctccttaccc aggctttgca actgatttgc aacaaccgct tacccctctt
    18001 ttactaagag cgaatggtcg tggtacaatt gtcgatacga tttacgaaaa acgtgtaaat
    18061 catgtttttg aactagcaaa gatggatgcg gatatttcga caacaaatgg tcatattttg
    18121 tacacgggtg gacgtgattt acgtggggcc agtgttaaag cgaccgactt aagagctggg
    18181 gctgcactag tcattgctgg gcttatggct gaaggtaaaa ctgaaattac caatatcgag
    18241 tttatcttac gtggttattc tgatattatc gaaaaattac gtaatttagg agcggatatt
    18301 agacttgttg aggattaaac cgtagaggtg tttatgaata tttggaccaa attagcaatg
    18361 ttttcttttt ttgaaacgga tcgcttgtat ttgcgtcctt tcttttttag tgatagtcag
    18421 gacttccgcg agatagcttc aaatccagaa aatcttcaat ttattttccc aacgcaggca
    18481 agtctggaag aaagtcaata tgcactggcc aattacttta tgaagtcccc tttgggagtg
    18541 tgggcaattt gtgaccagaa aaatcaacaa atgattggtt ctattaaatt tgagaagtta
    18601 gatgaaatca aaaaagaagc tgagcttggc tattttttga gaaaagatgc ttggtcgcaa
    18661 ggatttatga cagaggttgt tagaaaaatt tgtcagcttt cttttgagga atttggctta
    18721 aaacaattat ttatcattac ccaccttgaa aatgaagcta gccaaagagt tgctcttaag
    18781 tctggattta gtttgttccg tcagtttaag ggaagtgatc gttacacaag aaaaatgcgg
    18841 gattatcttg aatttcggta tgtaaaagga gagttcaatg agtaagcatc aggaaattct
    18901 aagctatttg gaggaattac cagtaggtaa aagggtcagt gttcgtagca tttcgaatca
    18961 tctaggagtt agtgatggaa cagcctatcg agctattaaa gaagctgaaa accgtggaat
    19021 tgtggagacc cgtcctagaa gtggaacaat tcgtgttaaa tcccagaaag ttgctataga
    19081 gagattaacg tttgctgaaa ttgcagaagt gacttcttct gaggttctgg ctgggcaaga
    19141 aggtttagag agagagttta gtaagttttc aattggtgcc atgactgaac aaaatatctt
    19201 gtcttacctt catgatgggg ggctcttgat tgtcggagac cgaacccgta ttcagttgct
    19261 agccttggaa aatgaaaatg cagttctggt tacaggggga tttcaggttc atgatgatgt
    19321 gcttaaactg gccaatcaaa aagggattcc tgttctaaga agtaagcatg atacctttac
    19381 cgtcgcgacc atgatcaata aagccttgtc aaatgtccaa atcaagactg atattctgac
    19441 agttgagaaa ctttatcgcc ctagtcatga gtatggtttt ctgagagaga cagatacagt
    19501 taaagattat ttggacttgg ttcgtaagaa tcgtagcagc cgtttccctg ttatcaatca
    19561 acatcaggtc gttgttggtg ttgtaaccat gagagacgct ggtgataaat caccaagcac
    19621 gacaattgat aaggttatgt ctcgtagtct attttgggtt ggattatcga caaatattgc
    19681 caatgtgagt caacggatga tcgcagaaga ctttgaaatg gtaccagttg ttcgaagcaa
    19741 tcaaactttg cttggcgttg tgacgcgacg agatgtcatg gagaagatga gccgttccca
    19801 agtttcggct ctaccaactt tttctgagca gattggacaa aagctctctt atcaccatga
    19861 tgaagtagtc attacagtgg aaccctttat gctagaaaaa aatggagttt tggctaatgg
    19921 tgtattggca gaaattctga cccacatgac ccaagattta gttgttaata gtggtcgcaa
    19981 tctcattatc gagcagatgc tgatctactt tttgcaggct gttcagatag atgatatatt
    20041 gcgcattcag gcacggatta ttcatcatac gagacggtca gctataattg attacgatat
    20101 ttatcatggt caccagattg tttcaaaagc aaatgtgact gttaaaatta attagaaact
    20161 aggagaaaag atgataacat taaaatcagc tcgtgaaatc gaagctatgg acaaggctgg
    20221 tgattttcta gcaagtattc atataggctt acgtgatttg attaagccag gcgtagatat
    20281 gtgggaagtt gaagaatatg tccgccgtcg ttgtaaagaa gaaaatttcc ttccacttca
    20341 gattggggtt gacggtgcca tgatggacta tccttatgct acctgttgct ctcttaacga
    20401 tgaagtggct cacgctttcc ctcgtcatta tatcttgaaa gatggtgatt tgctcaaagt
    20461 tgatatggtt ttgggaggtc ccattgctaa atctgaccta aatgtctcaa aattaaactt
    20521 caacaatgtt gaacaaatga aaaaatacac tcagagttat tctggtggtt tagcagactc
    20581 atgttgggct tatgctgttg gtacaccgtc cgaagaagtc aaaaacttga tggatgtaac
    20641 caaagaagct atgtacaagg gtattgagca agctgttgtt ggaaatcgta tcggtgatat
    20701 cggtgcggct attcaagaat acgctgaaag tcgtggttac ggtgtagtgc gtgatttggt
    20761 tggtcatggt gttggcccaa ctatgcacga agaaccaatg gttcctaact atggtattgc
    20821 aggtcgtgga ctccgtcttc gtgaaggaat ggtcttaacc attgaaccaa tgatcaatac
    20881 aggcgattgg gaaattgata cagatatgaa aactggttgg gcgcataaga ccattgacgg
    20941 tggattgtca tgtcagtatg aacaccaatt tgtcattacg aaagatggac ctgttatctt
    21001 gactagccaa ggtgaagaag gaacttatta ataaaaagtg aaaagactac tggaagttta
    21061 ttttgataaa aaatccagta gatcttttca taataaaacg cattgtatca agtgttaggg
    21121 gctgatatca tgcgtttttc tgcttttaag attttttcca actctgtttg taagcgcatc
    21181 ataacaaagg gtctaggatt cagggctctc ctcctatata ctattagtaa agtaaaacta
    21241 agggaggata ttttagtgtc gcagtctatt gttcctgtag agattccaca atattgtcgt
    21301 tttgattcta aaaagagaaa tggaattctg tttaacgttc gtattgccaa tcttaaattt
    21361 acctttttta gattagactt cctgcgaaat aaaatatggt atagtagttc tatgaatgat
    21421 gaagcaagta aacaactaac tgatgcagga tttaagcgtc ttgtt
//
LOCUS       DAVMGZ010000021        21450 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000021 DAVMGZ010000000
VERSION     DAVMGZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21450
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.022"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(84..503)
                     /locus_tag="VYQ93_000654"
     CDS             complement(84..503)
                     /locus_tag="VYQ93_000654"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV3335386.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDNKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(514..1920)
                     /gene="atpD"
                     /locus_tag="VYQ93_000655"
     CDS             complement(514..1920)
                     /gene="atpD"
                     /locus_tag="VYQ93_000655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV3335387.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(2006..2884)
                     /locus_tag="VYQ93_000656"
     CDS             complement(2006..2884)
                     /locus_tag="VYQ93_000656"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV3335388.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(2900..4405)
                     /locus_tag="VYQ93_000657"
     CDS             complement(2900..4405)
                     /locus_tag="VYQ93_000657"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV3335389.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVHFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(4420..4956)
                     /locus_tag="VYQ93_000658"
     CDS             complement(4420..4956)
                     /locus_tag="VYQ93_000658"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV3335390.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(4956..5450)
                     /gene="atpF"
                     /locus_tag="VYQ93_000659"
     CDS             complement(4956..5450)
                     /gene="atpF"
                     /locus_tag="VYQ93_000659"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV3335391.1"
                     /translation="MHVTVGELIGNFVLITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(5464..6180)
                     /gene="atpB"
                     /locus_tag="VYQ93_000660"
     CDS             complement(5464..6180)
                     /gene="atpB"
                     /locus_tag="VYQ93_000660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV3335392.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKIQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(6215..6415)
                     /locus_tag="VYQ93_000661"
     CDS             complement(6215..6415)
                     /locus_tag="VYQ93_000661"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV3335393.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(6490..7497)
                     /locus_tag="VYQ93_000662"
     CDS             complement(6490..7497)
                     /locus_tag="VYQ93_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV3335394.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLSNVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKRTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSKMEIIFIKRF
                     Q"
     gene            complement(7942..8340)
                     /locus_tag="VYQ93_000663"
     CDS             complement(7942..8340)
                     /locus_tag="VYQ93_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3335395.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(8893..9375)
                     /gene="greA"
                     /locus_tag="VYQ93_000664"
     CDS             complement(8893..9375)
                     /gene="greA"
                     /locus_tag="VYQ93_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV3335396.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(9446..11101)
                     /gene="mltG"
                     /locus_tag="VYQ93_000665"
     CDS             complement(9446..11101)
                     /gene="mltG"
                     /locus_tag="VYQ93_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV3335397.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPAKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(11182..11676)
                     /locus_tag="VYQ93_000666"
     CDS             complement(11182..11676)
                     /locus_tag="VYQ93_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3335398.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVDLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(11673..12137)
                     /locus_tag="VYQ93_000667"
     CDS             complement(11673..12137)
                     /locus_tag="VYQ93_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3335399.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKVIRLESPDELLSYFGMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(12147..13481)
                     /locus_tag="VYQ93_000668"
     CDS             complement(12147..13481)
                     /locus_tag="VYQ93_000668"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV3335400.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(13492..14109)
                     /locus_tag="VYQ93_000669"
     CDS             complement(13492..14109)
                     /locus_tag="VYQ93_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV3335401.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKLVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(14157..17255)
                     /locus_tag="VYQ93_000670"
     CDS             complement(14157..17255)
                     /locus_tag="VYQ93_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3335402.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKVLPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREKDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(17348..18514)
                     /locus_tag="VYQ93_000671"
     CDS             complement(17348..18514)
                     /locus_tag="VYQ93_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV3335403.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSIKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(18522..19616)
                     /locus_tag="VYQ93_000672"
     CDS             complement(18522..19616)
                     /locus_tag="VYQ93_000672"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV3335404.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            complement(19773..21416)
                     /locus_tag="VYQ93_000673"
                     /pseudo
     CDS             complement(19773..21416)
                     /locus_tag="VYQ93_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         6171 a         4755 c         3979 g         6545 t
ORIGIN      
        1 atcaaaaaag aaaggacaaa atttgtcctt tctcgagctt agctgacttc aacccactac
       61 agttgacaaa gagccatttt ttcttatagt ctatttccga cattaatacg gttaatagca
      121 cgttgcaaag caatcttagc acgacgttct tggtcaatca aatgtttgtc ttgtgcttct
      181 tcaattgcac gttctgcacg aagtttggca cgttctgcac gactgatatc gatatcacga
      241 gcacgttctg cagagtcagc gacgattgtg atcatatcat tggcaatttc aataacgcca
      301 ccgtttactg caatccagtt cacgtgatct ttattatcga tacgttttac ctttacttca
      361 tcaactgcta aaactgcaat catattttca tgtcgtggca agatccccat ctcaccatcc
      421 agagttcgaa ccgatacata gctggcatgg tgatcataga cgagaccatc tggtgtcacg
      481 atctggacag ttaactgagc catagatcac ctcttaaaat cccatttttt cagcttttgc
      541 aatcacatct tcgatagaac ctacaccacg gaaggcatct tctggcaagt ggtcgtattt
      601 accatcaagg atttccttaa agccacgtac agtttcagca actggaacat aagaacctgg
      661 ctgaccagta aattgttccg caacgttgaa gttttgtgac aagaagaact ggatacgacg
      721 ggcgcgagca accaaggtct tttcttcatc agaaagctca tccataccaa ggatagcaat
      781 gatatcttgc aattcatggt aacgttgaag gacacgtttt acttcagcag caactgcata
      841 gtgctcttct ccaacgattt caggtgccaa ggcacgtgag cttgaagcaa gtgggtcaac
      901 ggctgggtag atacccaatt gtaccaattt acgttccaag tttgttgttg aatccaagtg
      961 agcgaaggct gttgctggcg ctgggtcagt atagtcatcc gctggcacat agatagcctg
     1021 gatagaggtt acagaaccct tcttggttga tgtgatacgt tcttgcaatt gacccatttc
     1081 cgtagcaagt gttggttggt aaccaacggc tgatggcata cgacccaaaa gggcagatac
     1141 ttctgaacca gcctgagtga aacggaagat attatcgata aagagaagca cgtcttggcc
     1201 ttccacatca cggaagtatt cagcgattgt caaaccagta agggcaacac gcatacgtgc
     1261 tcctggtggc tcattcatct gaccaaagac catggctgtt ttctcgataa cgcctgattc
     1321 tttcatttcc cagtaaaggt cattcccctc acgagtacgt tccccaacac cagtaaatac
     1381 tgaaatacca ccgtgctctt gggcaatgtt gtgaatcaat tcttggatta agacggtttt
     1441 accaactccg gcaccaccga aaagtccaac tttaccacct ttaaggtaag gggcaagaag
     1501 gtcaataacc ttgatccctg tttcaaggat ttcagaagag gtagacaact catcaaaagt
     1561 tggagctttt ttatgaattg gctgacgctc tgcgtcttct gtaaaaggag cttccaaatc
     1621 aatggtatct cccaaaacgt tgaagacacg tcccaaagtt tctttaccta ctggtacaga
     1681 gattggacga cctgtgtcca atacttccat tccacgagtc aacccatctg ttgattccat
     1741 ggcgatagta cgaaccatac catctcctaa ctccaaggct acttcaagga cgatttttgt
     1801 ttttctttcg tcatttttgt agacgacaag tgcattgtta atctcaggaa gtttttcccc
     1861 tgctgcaaac aaaacgtcta caacgggacc gataacctga gcaattttac ctgaactcat
     1921 ctccttctcc tattctatat aagatactgt cggttcctag ctcaactagg tcctaagttc
     1981 attttcatac gagctggact agagcctatt ctaaggcact agcacctgct acgatttctg
     2041 taatttcttg tgtaatcgcc gcctgtctgg cacggttata ctgaattgtc aaatcattga
     2101 tgactttctt agcattatct gtcgctgttt gcatggctgt catacccgca gcattctcag
     2161 ctgtcttggc atcgataatg gcaccgtaaa tcatactttc tgcaaactga ggcaacaact
     2221 gctccagaat ttcttctcgg ctggtttcca attcaaaagt caagctgtac tcttcatccg
     2281 cttcatttgg atccaagtca acaatcggaa gcatttgttc cacacgcatt tgactggtta
     2341 gcgtattgac atggtggttg tagcaaacat aaagctcatc aaagagttca ttttggtaca
     2401 tttcaacagt ttttgaaata atcttacgaa cttgatcaaa gctaggttgg tctgccaagc
     2461 cacgtaattc ataaagtggt tgaataccgc gagccttaaa gaaatcagct cccatcccac
     2521 cgatacagat catttcaaaa cctttaccgt ctgggtggta ttcttctttc aactccataa
     2581 cagctttcaa aatagaggaa ttataacctc caaccaaacc gcggtctgaa gtgataacga
     2641 tatagcctgt cttcttcaca gaacggctaa tcaacatcgg attagttgaa gcaccagctc
     2701 cattaccatg aaggatatct gtcaaaagtt tacgcacttt ctgagcgtaa acttggaagt
     2761 tgcgagcagc ttcttcagaa cgacctagct tagcagccga taccatttgc atggcattag
     2821 tgatttgact cgtatttttt gttgaggcga tttttgtttt aatatcattt agagatactg
     2881 ccatctgaca cctctattct tattggaagc tagattgatt gagaaactct gtaatcgcag
     2941 catccaagac tgcttcttct ggcaagtctt ttgtatcacg aatggtttcc aaaatctctg
     3001 gatgttgagc atcaaagaag gtatggaact cttcctcgaa atgaacaata tcatctactg
     3061 gaacagtatc caagaaacca tgtgtcaaag cataaagaat ggttacttgt ttctcaacag
     3121 gtaatggttt gtgaacaggt tgtttcaaga cctcaacggt acgacgtcca cggttcaact
     3181 tagcctgtgt tgctgcgtcc aagtcagaac caaacttagt aaaggcttcc aactcacggt
     3241 atgaagcaag gtcgatacga agtgtaccag caaccttctt catggctttg atttgtgcag
     3301 aaccacctac acgagataca gatgaacccg catcgatggc tggacgaata cctgcattga
     3361 agaggccatc gccaaggaag atttgtccat cagtgataga aatcacgttg gttgcgatat
     3421 aggctgagat atctcctgct tgtgtctcga taaatggtag ggctgtaatt gatccaccac
     3481 caagttcatc agaaacttta gctgagcgct caagcaaacg gctgtggaga tagaaaacat
     3541 cccctgggaa ggcttcacga cctggaggac gacgaagcaa gagcgacagt tcacgataag
     3601 ctaccgcttg ttttgataga tcatcgtata caatcaaaac atgcttacct tgatacataa
     3661 attcttccgc catagcaacc ccagcataag gagctaggaa gagcaatgga gatggttgtg
     3721 aagcagaggc tgtcacaacg attgtgtagt ccaaggcacc gtactgacga agtgtttcta
     3781 cttgcgtacg aactgttgat tctttttgtc caatcgcgac gtagatacag atcatatctt
     3841 gatctttttg gttcaagatt gtatcaatcg caatggttgt tttccctgtc tgacggtcac
     3901 cgataatcaa ctcacgttga ccacgaccaa tcggtacaag ggcgtcaata gctttcaaac
     3961 cagtttgcaa tggttctgaa acagacttac gttgcataac accaggagct ggtgcttcta
     4021 ctggacgagt tttatcagtg tggatttctc caagaccgtc aactggacga ccaagcggat
     4081 ccacaacacg accaatcaga ctttcaccta cagggacttc catgattttc cctgtacggc
     4141 ggattgtatc gccttcacgg atatctgtaa agtcacctag gatgataata ccaacgtctg
     4201 ttgactccaa gttttgagcc ataccataag agccgttttc aaaattcaac aactctccac
     4261 tcatgacatt ttcaaggccg tgagcacgcg cgataccgtc cccgatatag gttacaacac
     4321 ctgtttcagt cacatcaaaa ttgggtttga aattttcaat ttgttgctta attaaagcgc
     4381 tgatttcttg tgcgttaatt gccaaaagaa caccactttc tatttcaaat tttctttaac
     4441 aactttaagt tgttgtttaa tactcacatc aattgtcttg tgattggcaa aaatgacaaa
     4501 accaccaatg agactttcat cgatttgttc ttttacactc cttactttca gagacatttt
     4561 tttctcaatc aaagggagca aacgagtctt ctgttcatca gttagaggat gagcagacgt
     4621 aatcgtcact tcaaatcgat ttgtttcttt ttcaagtcgg ttcaagcaat ctacaagcac
     4681 atcataaaaa agatttgctc tgtgattgta ggccagaacc tggataaagt tttgtaataa
     4741 aggtgacaca gaatcttgga aaaaagcaat tgttttttcc ttatccgact cgtctactgc
     4801 cacttgtttt aaaaaagaag gcagacctgt tttttcaaca acttgcttga tttgagtcaa
     4861 gtctgaaaag atacggtctt cttctccttt ttcaagtacc aattggacaa aaggcatgct
     4921 gtatttttca attaccttta ctgttttctt gtccattaag cttctcctag ctgatcgata
     4981 tactgatcaa tgagtgcttt atgggcatga ctgtcaaggt tttgtgagat gattttacca
     5041 gctaagctga tggtcaaatc tgcgacctca cccttaacac tctgtaaagc ttctacttta
     5101 ttttgagcaa tttcttgatt ggctttttct tttaagtgtc ctgcttctag tttagcatct
     5161 gctaagatat tagccttact ttgctcagct gtttcctttg cattttcaat gattgtctta
     5221 gcttctttac ggctaccagc caattcatct tcgcgttttt gagccaatac ttctgctttt
     5281 tgacgggctt cttcagctct gtcaatatct gaagcaattt tttcagctct ttcttcgaaa
     5341 atgcctgtaa tattagacca tgcaaatttt ttaatcaaga ctagcaaaag aataaaagag
     5401 ccagtgatta aaacaaaatt accaattaat tcacctactg ttacgtgcat cagttactcc
     5461 tttctattcc tcttcaatat taatcttatt ccctaaatac acagatgtca aaagagtaaa
     5521 aacataagct tggatgcagg aaataaagac agaaaatgca gtccaagcca aattagctcc
     5581 aaaggctact ggataccaat aaatagcttg gtgggaaaga agaagtaaca aactcgtcat
     5641 gacctctcct gcaaagatat tcccaaaaat ccgcaaagcc aaagataaga agtttgtaaa
     5701 ttcttccaag atattcatcg gtatgacaaa aacaggactc ataaaagatt ttatactttt
     5761 tttaaatcca cgacgacgaa cgctttctat atgtgtcaac aaaatgacaa gaaaagataa
     5821 ggttaagtca tactgtaaat tagcggttgg cgaactccac cagttagtcc catcgatcgt
     5881 ttgaatcttt gtcattaagc caaggttatt ggcaatcacc atgaaaagga ataaacaaag
     5941 gaaaaagagt gagtaatctt tcatgtagcg cgaaccaatg ttaggttctg taaatccaat
     6001 aacaaagtca tagacatact caagtacatt ttgctttcct ttgggtttca aggtcatatt
     6061 gcggcttgcc caataaataa agacaaaaat aactcccaca atcaacaaag tcatggctaa
     6121 catagtcaga ttgaagataa caggaccaat agagatgatt ggattaatac tttcttccat
     6181 ctaaaatcct cccttttctt ctcgttccat gtatttattt gataataaat gagaagacaa
     6241 gagttacaaa gaaagttcct tcaataaagg caacacctaa aaacatcaaa ctacgaaact
     6301 cagaaagcat atctggttgg cgtgctactg atttaaacag tccattcatc aataaacctt
     6361 caccgacaga tacgcccata caggcaatac ataagcctaa aaatgttaaa ttcataacaa
     6421 attctccttt aaaataaaat ttacttactt agtgtagtcc taaaatttaa atttgtcaac
     6481 ttttaaccgc tattggaagc gtttaataaa aattatttcc attttgctaa atttcaagtt
     6541 caagttagcc atccagaagt cttctctggg tgacttgtag tccaagcatt ttttaggata
     6601 gttgttaatc cacttttcga tgaatgcgac ttctttggga gtcattttct tggttctctt
     6661 aggtaaccat ctacgaatga gcctgttgtg attctcatta gttccccttt cccaagaggc
     6721 atagggatgt gcataataaa tgtgctcctc agaaaataca ttagacaagc gattgaattc
     6781 cgttccatta tctgccgtga tggaaagaat cttgtgttgt tttaagatga gttttagagc
     6841 ctgattgacc acctcagcac ttttatttgg aatcaatcgg atgatctgat gtctactttt
     6901 tcgatccgtc aagacaagca agcagtagtt tttcgctctc gtaagtagaa ccgtatcaat
     6961 ctcataatgc ccattctcca agcgaagatt gatagcttca gaccgctgtt cgatggattg
     7021 accagcaggt ttaaagttgg tgctagcctg tttcttaagc gcttttcctt ttctagggta
     7081 aagcagatcc tgtttgctta accccaattt tccatgatga atccaatagt aaatggttga
     7141 aattcccacg ttaacccctt tagccatcac catcatttca ggcgaaaatt tttggttatg
     7201 atagtggaga atcttttcct ttagttcctt ggtcaagctt gatttcttga ccgagcgctt
     7261 gcgattattt tcataagact gttgagcata gtcggcagaa taaacctctt tgaagcgccc
     7321 ttttccaaga cattgtcgga ctgtcccacg cttgatttca gtgtggatag tttgaggaac
     7381 ttttccaagc agagaggcaa tttctctatt tgatttccct tcttttttcc atctttcgat
     7441 taagcgacgg ctatcgattg tcaaatgttt ggcttttgta gtataattgt cttgcatctc
     7501 tgtgcctttc ttgtgtttgt ggttgaacaa caagtataac acagaggtgt tttcttatgc
     7561 ctacaagagc tttcattatt tccattttct tttggatttc actctattct gaaaaacttg
     7621 tgtatattta ctgaagctag caagtcttac ctgtaaattt aatgaaagca acacaaaatc
     7681 cgagagggga atctcggatt aatagataga gagtttttag tttaaataaa ttgtttaaaa
     7741 tatcaacaac atcacttctt ttcttaacct gataagtctt gattcctaat tttggggcta
     7801 cgattatatt gtcctcaata tcgtctagaa agacacaatt tctaggttat aactggtatt
     7861 tatcgatagt tactcatata catcagtcca cctccatact tatgtgcgag cctctctttg
     7921 tattatacct ccatactcac cttacagatt cttttggtaa taatatcttt gcctaatgta
     7981 gagatagtct tgcaaagaaa aaacttcctt gtagccatgt ttctgataaa agtccggtgc
     8041 ctggaactgg taagtattga caaaggcaaa acaacaattt cgattcttag cttcactttc
     8101 tgcctgttgc aatagttttg aaccgattcc ttgccctcgc agttcctctt ttacaaacaa
     8161 atactcgatt tctagccaat ttccaaaagt ctctgctatc aaacctgcca ggagattgcc
     8221 cttttcatct tcgacataaa gattaagtgg ctcactttca gcctcttctc tttttgaacg
     8281 gttataaaca cgaatcagat tccctatttc ttgcgattta tgtgattcct tattttccaa
     8341 tctaaagtat agtgaaatga aataaaacat gcgcaaatcg attaaggaat ttaatctaat
     8401 ttctaacaat gtcttagaaa tcaaagtgta ctattttaac ttcaatgcac tatacatcta
     8461 atactcaata aaaatcaaag agcaaactag gaaactagcc gcaggttgct caaaacactg
     8521 ttttgaggtt gtagatagaa ctgacgaagt cagctcaaag catggttttg aggttgtaga
     8581 tgaaactgac gaagtcagct caaagcatgg ttttgaggtt gtagatgaaa ctgacgaagt
     8641 cagctcaaag catggttttg aggttgtaga tgaaactgac gaagtcggct caaaacatgg
     8701 ttttgaggtt gtagatgaaa ctgacgaagt tagctcaaaa cactgttttg aggttgtaga
     8761 tgaaactgac aaagtcagta accatatata cggcaaggcg acgctgacgt ggtttgaaga
     8821 gattttcgaa gaatagcaaa atggaaaaag gagtgagtga agcacatcgc ctccccactc
     8881 ctttttctgt ttttaggctg ttttttcaac cttcaagatt tttacatcat agctaccaac
     8941 aggcgtttca attgttgctg tgtcacctgt tttcttgcca atcaaggcct gcccaattgg
     9001 gctttcattt gaaaccttac ctgcaaaggc atccgcacca gctgaaccta cgataatata
     9061 aacttcttct tcgtcctcgc caatttcttg gatggtgact gttttaccaa tcgctacttc
     9121 gtcctgggca actgcgtcgc tattgacgat ttcagcatag cggatttttg tttctaagct
     9181 agagatttgt ccttcgacaa aggcttgttc atccttagct gcttcgtact cactgttttc
     9241 tgaaagatca ccgtatgaac gggcaatctt aatgcgttct accacttctg gtcgacgaac
     9301 caatttcaat tcttctaatt ctttttcaag tttctccttt tcctcaaggg tcataggata
     9361 tgttttttct gccatttttc tcaactttct tctgataata ttttctaaag aaaattatgt
     9421 gaaatatcac ataattttag tttgtttagt ttaatttgct gtttacatgt tcagcgacat
     9481 tgcggtcgtg gtcttcttga ttgttagcat agtagacctt gccttctgtg acatctgcta
     9541 caaagtagag gttatcgctc ttagtttgat tgatgcttga ctcaatcgca tccagacttg
     9601 gactatcgac tggaccaggc atgagaccta catttttata aacattataa ggtgaatcaa
     9661 tgttggtatc aatcgcaaca tcctcagcta gactgatatt ttgccccagt tttccttggg
     9721 catacaagat tgcaatatta ctttgaagtg gcatatcacg attcaaacga ttgtagaata
     9781 cacctgcaat gagcttacga tcttctgtct tggcaccttc tttttcgacc aaggaagcaa
     9841 tggtcaacaa ctcattgaca gtcaagtttt tagatttgat agtactatag taaggagata
     9901 ggttcttatc catagcagct aacatctcat caatcaagct ctcaatagtt gtgctttcct
     9961 tgatagagta tgtagctggg aaaaggtatc cttccaaacg ataacgcgca ccgctgtctt
    10021 ttacaggcaa actttccagt aaagtaggat atttcgctac tgcttgactg ataaacgtct
    10081 catcttgaac tttagccaag aaagtctccg ctgtcaaaga ctctttgaag tcaccttgca
    10141 attgacccac agcttgagca atctgatcca aggtataacc ttctggaatt gtcaaagtcg
    10201 caagtacagg ttcttgcggt tcatctgttc cacctttttg caactctttg agtaagtctt
    10261 ctgtactcat actcttttgc aaattgtagt aacctgcttt caagtcggta taatttttat
    10321 acttggcata aaaactaaaa atcagaccat gctttaccaa accagctttt tcaagcgtcg
    10381 taccgatttc ttgaacgttt gaaccttctg gaattccaac cgtcacatat ttcttagaat
    10441 tagcatcgat aggtaataag gaatctagca cgtactggta accaaagtaa ccacctgctg
    10501 agagcaaggc aaggaataca aggaatgaaa tcacaaaagc tttcacatgt gacttcttct
    10561 ctttcttagg cttagcgggt tttgctcctt ctctacggct acgacgtgat gtatctctga
    10621 tgatatccac tgtttctgta gcaggcgtag gtgtttctgg acctgcttgt tctgcttttt
    10681 tctctgccgt cgtataggaa acagctaccc ttgttggggt ttcattgtat tctctttcaa
    10741 gtttcttagg tctaacagga cctggacctg gtcttgatcc actttcttcc gctggagaag
    10801 aaggtacatc ttgacttgga tgacttggaa caccaggagt ttctctttga atctcatctg
    10861 ctggagaagc tggtacacct tgacttgggt gagtaggcac ggtaggagct tttctcataa
    10921 tctcctctac cgttgacaag gaatcagcca tgagttcttc agctgaaggt tcatttgcag
    10981 gagtgcgaac tactgcctca tcttctttca gaacttcatc atagcctttt actttttcta
    11041 aatctctcag aatctgctct ttaaagctta atttctcttc ttctcttgac ttttcactca
    11101 aaagtttttc ctccttgttg ataatccata atattatatc ttaaaagtcc aaaaaaagca
    11161 atctatgata cttttcctaa cttatttttt catttcccag accatatcat accatgtttc
    11221 ccctgcaaag gttgactggg actggccttc gttgacaaat ccatgttttt cataataggc
    11281 gatgagatag tcatgacagg ttagattaat gccatctctc tcatcttcaa gagcaacttc
    11341 tttcaaggct gtcagcaatt tctgacccag tccgagtccc tgtgcctcct tggcaataga
    11401 cagacaggtc acagagatat aaccaccagg ctcatgacta tagtctttta tttcttctgt
    11461 aaaagactgg tcttgcagat ggcggtgcag gtcaactggt ccttcgatat aacccatgat
    11521 tcttccttct ttttcagcaa ccagaaaaga ggtctgaatt tctctcaaat gtgcttcaaa
    11581 gacagaagga ggaatggctt cttcgaccga aaaattttca aattcaagtt caacaatccg
    11641 atccaaatct tctaatcttg cttgtctgat tttcattgtt cctccagata aaagggatta
    11701 aaccaaatca tactatagcc ctggctagtt gcatagagca aagtttcttc ttcatcaaca
    11761 aaaccgttca ttccaaaata ggaaagcagc tcatcaggac tctccaaacg aatcactttg
    11821 taatccagct caactgccac ctctttcaag gctgcaagaa gaagtgttcc caggccctgt
    11881 ctctgatggt cagactcgat gactaaagaa tgtactttta gacattgcgg attgtctgac
    11941 tggggacttg ataaaatata gcctaaaagt tgattttcat ccctagctag aagaaaggta
    12001 tccgcacact tacggatact ttcttctaaa atatgggaaa gttgctgctt ttcagctgga
    12061 aaagacgagg tctgaagtgc ccctatctca ggcaaatcag acttgcttgc ctgaataatc
    12121 ttaattggaa tttccatggg aacatcctat tgaacattgc ttgtcaagtt agacaagaga
    12181 cgctcaaatg agtattcata ggtttggatg tctcctgctc ccataaagac gtaaacagca
    12241 ttgtcatggt ctaggagcgg agaaacattt tcaacagtaa tcacttggtg ttttttgttg
    12301 attttgttgg ctaggtcttc taccttaacg tcaccatgat ctacttcacg agccgagcca
    12361 taaatttgcg ctagataaac agcatctgct tggtttaaag catgggcaaa gtcgtccaac
    12421 aaggcaatgg ttcttgtaaa ggtatgcggt tgaaagactg ctacaatttc cttgcttggg
    12481 tatttctgac gagccgcatc caaggtcgca ataatttctg ttggatgatg ggcaaagtca
    12541 tcaataatca ctgtatcatt gacaattttc tcagtgaaac gacgtttaac accggcaaat
    12601 gttttcaagt gctcacgcac caagttcaaa tcaaatcctg ctgtgtaaag aagaccaata
    12661 acggctgtcg cattcatgat attgtgacga ccaaaggttg gaatgtggaa ttgccccaag
    12721 ttttgtccac ggaaatgaac ggtgaaggtt gaaccagttg ttgaacgaag aagatcacta
    12781 gctacaaagt cattgccttc agcttcaaaa ccataataat aaattggtgc atcagacgta
    12841 atcttacgca attcagcatc ttcaccatag acaaaaagac ccttggtaat ttgtttggca
    12901 tagtcgttaa aggcattgaa aacatcctcg agacttgtga aataatctgg atggtcaaag
    12961 tcaatgttgg tgataataga gtattctggg tggtaaggca tgaagtgacg ctcatattcg
    13021 tcagattcaa agacaaaata tttggcatta gccgaaccac gacctgtccc atctccaatc
    13081 aagaagctgg tatctgtaat gtgagacaag acatgagaca acatacctgt cgttgaagtt
    13141 tttccatgtg ctcctgctac tcccatgcta acaaagtcac gcataaagct acctagaaac
    13201 tcatggtaac gtttgtagct gataccattt tggtccgcat aggcaatttc gacgttgtta
    13261 tctggacgaa aggcatttcc agcgataatt tccatatcac cgtctagatt cttttcatca
    13321 aaaggaagaa tggtaattcc tgcctgctca agaccgcgtt gggtaaagta gtacttttca
    13381 acatctgatc cctgaacctt gtgccccatc tggtgcaaca tcaaggccaa ggcactcatc
    13441 cctgatccct taattccgat aaaatgatat gtctttgaca tgttttctcc cctattctgt
    13501 cattctggtc agattcaact cttgggcaac ccgacgttct tgttctgttt gtttactttt
    13561 tttattgtag atttggctct tctttagaaa atcataattg tttttctttg gagcaggtgc
    13621 tgacacttct tcattcttgg tagggataga atgaacttct tccgccaaga tataatgaga
    13681 ctgggtcaat ttttggctat atttgacaaa ttcaccagga ttttcctttt ggaaaggagt
    13741 tgtcggttga ttgccctgtc taactagact gggctgagaa tgacgtctcg caaggctgaa
    13801 atcctgagtt aggtagttag cagagcgttt ctttttcaag tccgcacgcg cttcttcacg
    13861 cgccacctcc gcatagctct ttccttcttt tttaacccct aaaggagcct ttttaggttt
    13921 ttcgacttgc ttttcaatcg gttttactag tttttcttca gtaataggag cccattctaa
    13981 ataattttta tctcgatact cacccttgat attactgatc agatcagact catcatagag
    14041 attcatgact ggcatttcag tcaacatgac ctcgtcatct gacaccaatg gaaatcgttc
    14101 ttgtttcatt ttctatttcc tttcaacact tcattatagc gtattgtctt gatttttcaa
    14161 gtgctggctt cagaaattcc caaaatttct ctaatttctg ctagggtcag actaccacgt
    14221 gactctgtac catccaatac ttgtgacacc agatgtttct tttgttcttg gagttcctga
    14281 attttttctt caatggttcc cttggtcacc aagcgataga cctcaaccgt ttcttcctga
    14341 cccatccgat gggcacggcc aatggcttgc gcttccaccg caggattcca ccaaaggtca
    14401 accaagatca ctgtatctgc acctgtcagg ttcagaccga ccccaccagc cttgagggaa
    14461 atcagaaagg catctctttc tccttggtta aaggccttgg tcatgtcttg tctttccttg
    14521 gctggggttg aacccgtaat tttaaaggat gtcaggccca agtctggcag ttcttgttca
    14581 attttttcca acattccctt gaactgagag aaaatcaaga cacggtgtcc gccgtctgcc
    14641 acctgtacca gtaggtctcg gagactatct agtttgccgc tggctccctg ataatcttcc
    14701 ataaacaggg caggagtgtc acatatttga cgcaagcgca tcaaaccaga taaaatttcc
    14761 acacgacttc gctgaaattc ctgttctgac acttgagcca gatggtctcg catctgttgt
    14821 aactgggcaa ggtaaatagc cttttgctgg tcttccagtt catttttata aaccacctca
    14881 atcaagtctg gcaattcagt cagaacttct tctttcttgc gtcgcatcac gaaaggcttg
    14941 ataaactgag ccactcgctc tgctggcaat ttcataaatt ctttcttgct tggcaaaagt
    15001 ccgggcatga cgatttggaa aatagaccac aactcaccca gatggttttc aatcggagtt
    15061 cctgacaagg caaagaccga cggcaccaca aattgtctca aggtctgggc aatcttggtc
    15121 tgggcatttt tcatgacctg agcctcatct aagaaaagga agtcaaaggc catcccctga
    15181 taaaactcac tgtcctgacg gaaggtggca tagctagtca catagatttg atggctctcg
    15241 gcaagaatct cctcacgact tgctttcaaa ccatgaacaa cagccacatc caactgtggg
    15301 gcaaatttct gaaactcatc tgcccagttg taaatcaaac ctgacggagc gagaatcaaa
    15361 acccgacttt cttttgtcac ttgactagtc aaaaaagcaa tggtctgaag ggttttccca
    15421 agtcccatat catcagccaa aatcccacca aaaccataat gatggagcat ctgcaaccag
    15481 ccaattccct tttcctgata atctcgcaag tcagcctgaa cctgagttgc ctgtcgagga
    15541 aagtcctctg gatgtgtcaa atcctgggcc agattctgga attcttgtga aaaagaaaca
    15601 cggtctcgcc cttcaaagag atgagctaaa ctgtaggcca aggatttccg agcctgcaag
    15661 gtcccatctt ttaattcaaa ttgccccagt tcctgtagat tttgccgaat tttcttggtt
    15721 tcttcatcga aaaagtaaac ttgattagac gaatcaatat aaaaatcctg attggcaacc
    15781 aaggcctgca tggcttggtc gatttcctcc tgggcaatat tttgaaaatc aaactggatt
    15841 tccaagagac ctcccttgga ggcaatctgc acctgaggac tcgctaggct ataaagctct
    15901 tctagtttat ctgataggtc aacatgcccg agtttttcaa agactggaat gatatcatga
    15961 aaaaaatgat agacagactc cgcttttaag gcctgacgcc aagattgaaa atcggcttca
    16021 aagcccgcag ccaaacagac ttggaaaatt ctttcttcta agtccgcgtc acttgaaaat
    16081 ggtaattctt ctagctcttg tcggctagat acctgtctat ttccataatc aaactgaatt
    16141 tctaaacgaa tccgattatc tttttccctg tcaaagtaaa aagagggcgc aaaagttttg
    16201 atttgtagac gttctggagc tgaaacggtg cccatctgga taaaaagagt cagacaggag
    16261 gccaatttgt ctcgatcact gctatcaaat tgcaagtatt tctttccttg ttgacccaca
    16321 ggtaacactt taatttcctt gagaagacgc atctgctggt ctgttaaaaa ataaacctga
    16381 cctttatgga aaagtactgc tccctgataa aagacattaa ccctaggact ctcactgatt
    16441 tccatttcaa aataatccga gtattctgtt actgtaaagg caaatagatt ggcatcagca
    16501 tgcatatcct gaaaaagcag ggtttggtag ctatccactt gatggtcaaa ttgaaaatgg
    16561 ggcaaggcca tcagtaaatt cacaccctgc tcaaaaaagg tcagagggaa aaagaggtgc
    16621 cgaccttggt tttggaaaaa gaggtctgga accagccctt cctccgttag tccgtgcaag
    16681 aaagtcaaaa gttcttggct ggcatcatca aaggcttccc aagaaagaga ctcctcataa
    16741 atcttgccaa tcatatacga ctttctctgc tcgacaatcc ttaaaaaaag tggaatatcc
    16801 cgaatgacat agtatttttg gctattgatt tggccgattc tcagagtcca caagatatga
    16861 ttggttcctg cttccacctg acccacagct gataactcat aggcgcattc tgattttgga
    16921 gataaaattc gatccaaaaa cttgccaccc aaggtcacct tggtttcaac agcctctttt
    16981 tcttcatgac cttcttccag actccacaag atttcctgac cacgctcatc atttttcaga
    17041 aaatgctcta gcgctgccaa atgcacacag tagcccctct tttgaaaaaa atcacaggca
    17101 caaaaaacca aatcatcctc taaactatag cgcagttctt cttctgcaac gcgagcgtag
    17161 agccgattgt tcttttcctt gatgatatca accttaccag tttcataaag ggcaacacct
    17221 tcgatacgaa ctttccccgg aatcaattta gccatatttt cacctttacc ttatcttttt
    17281 attataccat attttctcct atgaaaatag ccttctagga agacttttct cctagaaggc
    17341 tggattttta acgtttggca aaagtagcca caatccgctg acagacttct tgcaacagag
    17401 atttgggcat agctatattg atgcgggcat ggagacttcc ttcctctcca aaatccaaac
    17461 cacggttgag gataaccttg gcttcatttc tcaacaactc ttgcaatgtt tcatcagtca
    17521 ggtcataagc tgaaaagtca agccaaatca agtaggtacc ttgcggtttc atgaccttga
    17581 ttttagtctc ttttccaaat agatccacca cataattgat gtggtcttca aagacttgct
    17641 tgagttcctc tagccaatct ttaccgtatc gataggcagc ttctgtcgcc aaataaccca
    17701 agcctgaaat ttcatgctga ttattggcca acaggcgttt ctggaaagcc agtctcaact
    17761 taggattttc aatgactgca taggaatttt ttgttccagc aatattaaat gttttagtgg
    17821 cactgctcaa gacgatagca aaatttttga aggcaggatt gatggtattg aaagactggt
    17881 gtttgtgacc aaagagggtc aaatcttggt gaatctcatc cgaaactaac aaaacaccgt
    17941 gtttttggca gagttggcca atcttctcca acacttcttt ttcccaaaca cgtccaccag
    18001 gattgtgagg gttgcaaaga atatagagtt taacctcctc ttccaccaaa tccttttcaa
    18061 gttggtcaaa gtcaatctca aacagaccat ccttttccac taaggaatta gtaatcaatc
    18121 tacgattatt caacttgata ctgcgagcaa agggtgggta gacaggcgtg ttaattaaaa
    18181 ccgcctcgcc ttcttttgta aaggtttgaa tagctgttga gatggctggt accacaccct
    18241 cgataaagac aagagcctct ttgtcaaagt tgtaaccgta ttgtgtagct tcccactttt
    18301 gaacttcctt aattaagtct tcactggcat aggtataacc ataaaccagt tggtctgcgt
    18361 aagtttgcac ggcttggcgg atttcaggca agaccacaaa gtccatatcc gctatccaag
    18421 ctggtagaac ttcactatcc gtttctgttt ctttccattt ataggtatgg tgccctaaac
    18481 ggttgggcag gcttgtaaaa tcatattttc ccatctttgt cttatccttc taaggcttgg
    18541 cgcaaatctg caatcaaatc tctagcatcc tcaatcccaa tagacaaacg caagaggtca
    18601 tctgtcaaac cataagaatg gcgtacttct gctggaatat cagcatgagt ttgagtcgtt
    18661 ggataagtaa taagactttc cactccaccc aaactttccg caaaagagaa gaccttgaga
    18721 ctgttcaaaa tatgaggaat gcgtgtttca tcggctactt taaaggaaat catgcctcca
    18781 cgaccagtgt agagaacttc cttaactgct ggagaatcct tcaaaaaggc aaccacttct
    18841 tgggcgttag ctgttgagcg ctccatacga agagacaaag tcttgagacc acgaagcaac
    18901 tggtagctgt caaatggaga caagactgcc cctgttgtat tgagattgta aaaaagcttc
    18961 tcgtatagtt ctaaactatt ggtcacaacc actccagcca agacatcatt gtggcctgct
    19021 agatacttgg ttgctgaatg gagaacgata tctgctccat cttcaatcgg acgttggtag
    19081 atagggctgt agaaggtatt gtccaccacc actttggcac ccttagcatg agccaatttt
    19141 gctagttttt cgatatcaaa ttccaacatc aagggattgg ttggggtttc gatatagaga
    19201 acatccacat ccttttctaa ctcggcaatc aactcttctt ctgtattggc ataggtaaaa
    19261 tggaaatgac cttcctgctc cacttggtta aaccagcgaa aagaaccacc gtaaagatca
    19321 cgcactgcca agaccttact tcctactgga aagacgctaa aggccagtac aatagctgac
    19381 atccctgagc tagtcgctag ggcatagtct gctgactcaa tagccgccaa gacttcctca
    19441 gccttactac gagttggatt tttagtgcgc gtatagtcaa acccagtaga tcgaccaaac
    19501 tctggatgct gataggtcgt tgaaaaatga agcggtgtca ccaatgcacc tgtcgcttca
    19561 tctgacttaa tacccgcctg ggccaaaatt gtgttaatgt gtaattcctt gctcatacaa
    19621 tacctccaaa tctatagtaa ctattgtacc acttattttc atcaactcat tttcttcttt
    19681 tcaagagcta gttatagttt caaactatat aaaaagggag tttttcctgc tccctttaat
    19741 agactataaa atggtgaatc tcaaaagaca cttcactcta tcatttgctc ctgcacaaaa
    19801 cgagcataac gctcatgatt ttccagtagt tccttatgag ttcctgagcc agtgattttc
    19861 ccctcctcta agaagaaaat acaatccaca tcttttaccg ttgacaaact atgcgctata
    19921 atcacaaccg tcttctcctt taatacagaa tagaggctac tgataatcgc atactcagaa
    19981 tccgcatcaa gattagcagt ggcttcatca aatataagaa tttcagcatc ttttaagtag
    20041 gctctagcta tttgaagtct ttggctttgc ccccctgaca agaatcgtcc gcgttcacca
    20101 acttcagtat ctagtccctc tttcatggag cgaatctcat cacctagtga tactaagtct
    20161 agcactttca tcaattcatc atcagttact aagcgattca aaccgagaca aagattgtca
    20221 cgaatactgc cagataagac tgcattattt tgtgaaaccc aagcgatttt acttctccat
    20281 tcttttaagt taaaatcata tatacttgat tgctccatta gaatatctcc tgaaagcggt
    20341 ttataaaacc gctctaacaa acgcacaatc gttgattttc ctgatccaga tggtccaaca
    20401 aaagcaattt ttttcccctt gaaaattgaa caagtaatat cctttaagac aggtcgattt
    20461 tcatcataac caaaatagac atggttaaaa ttcaaccttt atcctgatac cgattttcct
    20521 ccctcaaatt tttctttagg aactgcaagc aagttctcca gtgcaactga agatcccttg
    20581 ctcctagaat aaacagttac aaaattagct atattactaa taggattaag taattgaaag
    20641 aggtaaatca aaaacgaaac caaggttccc acagatatat atcctgcgct gacccgataa
    20701 cccccatagg ttagcatcac agctatagtc gcaaagataa ataagagagc aaacggggtc
    20761 tcaaaagaag taaccctatc tgatttcagt gaattgtttt gtaccctttc aatacaatta
    20821 tccaaaacat cctgtacact tttctctgct tggttagtct taattaattc atgttcttga
    20881 atcttttcag tcaattgccc tgttaaaaat ccgatttcct cctgtaaacg acgactatac
    20941 ttttcactga tattggaaag gggcaagata ataaacatca tacaaggaag agtgatgaat
    21001 aaaagtagag aaagattcca atcaagacta aataagacta caatggaacc aagtaccata
    21061 actaaactca gaataatatt tgggaaagtc gtaattaaaa actcacgaat gacactcatg
    21121 tcattgacaa tggcagaagt caactcccca ctttggctct tatcaaagaa ggatttctct
    21181 gcataaatca acccctctat cacttttttc ctgatttttg ctatcttttt ttcacccgat
    21241 tgactaaaca gatagtaacc aatagaagaa aacaaggctt gaccaataaa aatcaaaaac
    21301 gattgaaata ctttggaacc tatattttca atagaactcc catctattaa accttttaag
    21361 ataaggggaa gcaacaaagc aagtagacta gacagaacaa ggaagaaact ccccataatc
    21421 accttagtat ctactcttaa taattttaat
//
LOCUS       DAVMGZ010000022        21075 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000022 DAVMGZ010000000
VERSION     DAVMGZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.023"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(30..1367)
                     /locus_tag="VYQ93_000674"
     CDS             complement(30..1367)
                     /locus_tag="VYQ93_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV3335405.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(1377..1643)
                     /locus_tag="VYQ93_000675"
     CDS             complement(1377..1643)
                     /locus_tag="VYQ93_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV3335406.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(1908..2294)
                     /gene="rplQ"
                     /locus_tag="VYQ93_000676"
     CDS             complement(1908..2294)
                     /gene="rplQ"
                     /locus_tag="VYQ93_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV3335407.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(2306..3241)
                     /locus_tag="VYQ93_000677"
     CDS             complement(2306..3241)
                     /locus_tag="VYQ93_000677"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV3335408.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(3284..3667)
                     /gene="rpsK"
                     /locus_tag="VYQ93_000678"
     CDS             complement(3284..3667)
                     /gene="rpsK"
                     /locus_tag="VYQ93_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV3335409.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(3685..4050)
                     /gene="rpsM"
                     /locus_tag="VYQ93_000679"
     CDS             complement(3685..4050)
                     /gene="rpsM"
                     /locus_tag="VYQ93_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV3335410.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(4068..4184)
                     /gene="rpmJ"
                     /locus_tag="VYQ93_000680"
     CDS             complement(4068..4184)
                     /gene="rpmJ"
                     /locus_tag="VYQ93_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV3335411.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(4209..4427)
                     /gene="infA"
                     /locus_tag="VYQ93_000681"
     CDS             complement(4209..4427)
                     /gene="infA"
                     /locus_tag="VYQ93_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV3335412.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(4544..5182)
                     /locus_tag="VYQ93_000682"
     CDS             complement(4544..5182)
                     /locus_tag="VYQ93_000682"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV3335413.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(5333..6643)
                     /gene="secY"
                     /locus_tag="VYQ93_000683"
     CDS             complement(5333..6643)
                     /gene="secY"
                     /locus_tag="VYQ93_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV3335414.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(6656..7096)
                     /gene="rplO"
                     /locus_tag="VYQ93_000684"
     CDS             complement(6656..7096)
                     /gene="rplO"
                     /locus_tag="VYQ93_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV3335415.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(7241..7465)
                     /gene="rpmD"
                     /locus_tag="VYQ93_000685"
     CDS             complement(7241..7465)
                     /gene="rpmD"
                     /locus_tag="VYQ93_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV3335416.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(7437..7931)
                     /gene="rpsE"
                     /locus_tag="VYQ93_000686"
     CDS             complement(7437..7931)
                     /gene="rpsE"
                     /locus_tag="VYQ93_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV3335417.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(7949..8305)
                     /gene="rplR"
                     /locus_tag="VYQ93_000687"
     CDS             complement(7949..8305)
                     /gene="rplR"
                     /locus_tag="VYQ93_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV3335418.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(8389..8925)
                     /gene="rplF"
                     /locus_tag="VYQ93_000688"
     CDS             complement(8389..8925)
                     /gene="rplF"
                     /locus_tag="VYQ93_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV3335419.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(9117..9515)
                     /gene="rpsH"
                     /locus_tag="VYQ93_000689"
     CDS             complement(9117..9515)
                     /gene="rpsH"
                     /locus_tag="VYQ93_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV3335420.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(9770..10039)
                     /gene="rpsN"
                     /locus_tag="VYQ93_000690"
     CDS             complement(9770..10039)
                     /gene="rpsN"
                     /locus_tag="VYQ93_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV3335421.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(10057..10599)
                     /gene="rplE"
                     /locus_tag="VYQ93_000691"
     CDS             complement(10057..10599)
                     /gene="rplE"
                     /locus_tag="VYQ93_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV3335422.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(10623..10928)
                     /gene="rplX"
                     /locus_tag="VYQ93_000692"
     CDS             complement(10623..10928)
                     /gene="rplX"
                     /locus_tag="VYQ93_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV3335423.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(11006..11374)
                     /gene="rplN"
                     /locus_tag="VYQ93_000693"
     CDS             complement(11006..11374)
                     /gene="rplN"
                     /locus_tag="VYQ93_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV3335424.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(11400..11660)
                     /gene="rpsQ"
                     /locus_tag="VYQ93_000694"
     CDS             complement(11400..11660)
                     /gene="rpsQ"
                     /locus_tag="VYQ93_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV3335425.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(11685..11891)
                     /locus_tag="VYQ93_000695"
     CDS             complement(11685..11891)
                     /locus_tag="VYQ93_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV3335426.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(11901..12314)
                     /gene="rplP"
                     /locus_tag="VYQ93_000696"
     CDS             complement(11901..12314)
                     /gene="rplP"
                     /locus_tag="VYQ93_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV3335427.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(12318..12971)
                     /gene="rpsC"
                     /locus_tag="VYQ93_000697"
     CDS             complement(12318..12971)
                     /gene="rpsC"
                     /locus_tag="VYQ93_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV3335428.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(12984..13328)
                     /gene="rplV"
                     /locus_tag="VYQ93_000698"
     CDS             complement(12984..13328)
                     /gene="rplV"
                     /locus_tag="VYQ93_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV3335429.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(13340..13621)
                     /gene="rpsS"
                     /locus_tag="VYQ93_000699"
     CDS             complement(13340..13621)
                     /gene="rpsS"
                     /locus_tag="VYQ93_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV3335430.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(13725..14558)
                     /gene="rplB"
                     /locus_tag="VYQ93_000700"
     CDS             complement(13725..14558)
                     /gene="rplB"
                     /locus_tag="VYQ93_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV3335431.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(14576..14872)
                     /locus_tag="VYQ93_000701"
     CDS             complement(14576..14872)
                     /locus_tag="VYQ93_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV3335432.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(14872..15495)
                     /gene="rplD"
                     /locus_tag="VYQ93_000702"
     CDS             complement(14872..15495)
                     /gene="rplD"
                     /locus_tag="VYQ93_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV3335433.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(15520..16146)
                     /gene="rplC"
                     /locus_tag="VYQ93_000703"
     CDS             complement(15520..16146)
                     /gene="rplC"
                     /locus_tag="VYQ93_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV3335434.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(16363..16671)
                     /gene="rpsJ"
                     /locus_tag="VYQ93_000704"
     CDS             complement(16363..16671)
                     /gene="rpsJ"
                     /locus_tag="VYQ93_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV3335435.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(16937..17554)
                     /locus_tag="VYQ93_000705"
     CDS             complement(16937..17554)
                     /locus_tag="VYQ93_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV3335436.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(17551..18141)
                     /gene="nrdG"
                     /locus_tag="VYQ93_000706"
     CDS             complement(17551..18141)
                     /gene="nrdG"
                     /locus_tag="VYQ93_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV3335437.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(18134..18640)
                     /locus_tag="VYQ93_000707"
     CDS             complement(18134..18640)
                     /locus_tag="VYQ93_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3335438.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            complement(18684..18824)
                     /locus_tag="VYQ93_000708"
     CDS             complement(18684..18824)
                     /locus_tag="VYQ93_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335439.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(18836..21043)
                     /gene="nrdD"
                     /locus_tag="VYQ93_000709"
     CDS             complement(18836..21043)
                     /gene="nrdD"
                     /locus_tag="VYQ93_000709"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV3335440.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
BASE COUNT         5791 a         4720 c         3969 g         6595 t
ORIGIN      
        1 aaatagaact taaaatttct cctattttct taatttttaa aactatgaat tggtgctggg
       61 atttgtccac cgcgagagat gaagtcgaca gacgaagccc cattaacctt cataacgggt
      121 gcagttccta atagaccacc aaactcaatc atatcgcctt ctcttccttt gggaatgata
      181 cgaacagctg ttgttttcat gttgataaca ccgattgctg cttcatccgc aatcatagcc
      241 gcaatagttt cagcaggcgt atcttctggg atggcaatca tatccaatcc aacagaacag
      301 atagccgtca tagcttctag tttttctaaa ttaagagagc cattttgcac tgcagcaatc
      361 attccttcat cctcagaaac agggataaag gcaccagata gaccaccgac ctggttacag
      421 gccatcactc cacccttttt aacttggtcg ttcaagaggg ccaaggcagc tgtcgttcca
      481 tgcgtgccaa ctgtttctag ccccatttcc tcaaggacac gtgccacaga gtctccaacc
      541 gcaggggttg gtgccaaact caagtccaca ataccaaact ccacacccag tctctcactg
      601 gccatttgac caaccaattg accgatacga gtgattttaa aggcagtttt cttaactgtt
      661 tcggctacta catcaaagct ctgtccacga actttttcca aagcacgttt cacaacacca
      721 ggaccagaaa ctccgacatt gatgataaca tctgcttccc caacaccatg aaaggcaccc
      781 gccataaatg gattgtcctc aacagcatta gcgaatacaa ccaacttggc cactcccata
      841 tctgaaagat ttgctgtttc cttgataatt cgtcccatat ctgccacagc cgtcatatta
      901 ataccagact tggttgagcc gatattgact gacgagcaga ccttatccgt ctcagccaaa
      961 gcgcgaggaa tggaattgat gagaatctca tctccctttt gataaccttt ttgtactaag
     1021 gcagaaaaac caccaataaa gtccacacca atctctttcg cagccttatc aagcgctttt
     1081 gccagaacca cgtagtccgt cgcatctgtc gctgccccaa tcagagaaat aggtgtcacc
     1141 gatacacgct tattaacgat aggaattccc aactcagccg caatttcatc accaacagct
     1201 actaaattag ccgcctttgt cgtaattttt tgatagattt tctccgcagc acgattgata
     1261 tttgggtcga tacagtccaa aagagaaatc cccatggtaa tggttctaat atcgaagttt
     1321 tgctcctcaa tcatggcgat ggtttcagta acttgtctaa tatccatgat gacctcctag
     1381 atattataca tagcttcgaa aatcgctgca ctctgaatat tgatttttac attcaaagtt
     1441 tgcccaaaag cttcaaattc attacgaaga taggtaaaat cttgcttttc atcactagat
     1501 acaacagcca tcatcgtaaa atattcatcc aatacagttt gagagatatc gtcaatattc
     1561 aatcctaatt ctgcaatttt accagaaaca cctgcaacaa ttccagattt atctttacca
     1621 acaacagtta taatcgcttt cataagtaaa ctccaattct tcttaaaata agaaacctta
     1681 acattaaaca ggattctttt caaatagtat agcataattc taacgaaaat actcaaaaac
     1741 gcctgcttac gaagttgttc ttaagaaaaa aacaatttcg taaacaagcg tctactatcc
     1801 caacttatga tgagtgttcc cgctaggacc gaaatcctag cggtagacca gagctagact
     1861 aagagcacaa gactccatca tcataacact caacaaaatt gatgatttta tactaattcg
     1921 atgatcgcca ttggcgctgc atcaccacga cgtggttcag ttttaaggat acgagtgtat
     1981 ccaccgttac gttcagcata acgaggtgcg atttctgaga acaatttttg aagtgctgta
     2041 gtagaagtgt acttatcagt tgcttcatca tagttttcag atgcgatttc attacgtacg
     2101 aaagcagctg cttgacgacg tgcatgcaaa tcaccacgtt tacctagagt aatcattttt
     2161 tcaacagttt tacggatttc tttagcacga gcttcagttg tcacgattga ttcgttgatc
     2221 aaaaggtcag ttgtcaaatc gcgaagcatt gctttacgtt gtgagctagt gcgtcctagt
     2281 ttacggtaag ccatgtattc ctcctttatt tatcttttaa tccaagaccc aaatcaatga
     2341 gtttgagttt cacttcttcc aaactcttgc gtccaagatt tcgtactttc atcatctctg
     2401 cttcagattt ttctgtcaaa tcatgcacag tattgatacc ggcacgtttt aaacagttgt
     2461 atgaacgcac agacaagtcc agttcctcaa tcgtacgatc taaaatacgg tcgtcagatt
     2521 cagtatcagc ttctttcatc acttcagttg acttagcaat ctcagtaaga tttgtaaaca
     2581 aatcaagatg ttctgtcaaa atacgtgctg aaagccctaa agcatcttct ggaataattg
     2641 ttccatttgt caagatttca agggttaatt tgtcgaaacc atcattgcta cctacacgag
     2701 caggttccac ttgatagttg acttttgtaa ctggtgtata aatagaatct acagcaagtg
     2761 ttccaactgg tgcattatcc tttttatttt catcagcagg tacatatcca cgaccactgt
     2821 taacagtcat agtcgctttt agagaagaac cttcaccaat tgtaaagaga taatgatctg
     2881 gatttacaat ttcaatatcg ctatctgtca aaatgtcacc agctgttact tcagcaggac
     2941 cttcaacatc cagttcgatg attttttcgt cttcaacgta cgatttcact gcaattcctt
     3001 taatgttcag aatgatttgc ataacgtctt cacgaacacc tggaactgtg tcaaactcat
     3061 gtaacacacc atcaatgttg atagatgtca cagctgctcc tggtagagaa gctagaagta
     3121 cacgacgaag agagttacca agagttgtac cgtagccacg ttcaagtggt tcgattacaa
     3181 acttgccata atctttattt tcatcaattt ttgttatatt tggtttttca aactcgatca
     3241 tttagttact ccctcttaaa cgaaaagcag tgtaatgcga tgattataca cggcgacgtt
     3301 ttggaggacg agcaccattg tgtggcactg gagtcacatc acgaattgct gttacttcaa
     3361 gaccagcggc agcaagcgca cgaatagctg actcacgacc agaacctgga ccttttacag
     3421 taacttcaac tgatttaaga ccgtgttctt gtgcagattt agcagcagct tcagaagcca
     3481 tttgagcagc gaatggtgta gatttacgag aacctttgaa accaagagca ccagctgatg
     3541 accaagcaat tgcattacca tgcacatcag taatcataac aatagtgtta ttaaatgtag
     3601 cgtgaatatg agcaatacca gattcgatat tctttttcac acgacgttta cgtgttggtt
     3661 tagccaagac ttttacctcc tatattattt tttcttacca gcaatcgcaa cagctttacc
     3721 tttacgagtg cgggcgttgt ttttagtgtt ttgtccacgg acaggaagtc cacgacggtg
     3781 acggatacca cggtatgaac cgatttccat caaacgtttg atgttcaagt ttacttcacg
     3841 acgaaggtca ccttcaactt tgattgcatc cacttcacga cggatagcat cttcttgatc
     3901 tgatgtaaga tcacgtacac gaacatcttc tgagattcca gcagcagcca aaattttctt
     3961 agatgttgca agtccgatac cataaacata agtcaatgag attactacgc gtttgtcatt
     4021 tggaatatca actccagcaa tacgagccat gttttctcct ttctatctta tccttgacgt
     4081 tgtttgtgtt ttggatttgc tgggcaaatt accataacac gaccattacg acgaataact
     4141 ttacagtatt cgcaaattgg tttgaccgat ggtcttactt tcatttctta tccctccaag
     4201 tttttcgatt atttaaagcg gtaagtgata cgtccacgtg tcaagtcata tggactcatt
     4261 tcgacagtaa cacgatctcc cgctaaaata cgaatatagt ttttacgaat tttaccagaa
     4321 actgttgcta aaatctgatg tccattttca agttcaaccg taaacattgc attcggcatt
     4381 gtatcaacta ctttgccttc aacttcaatc acatcgtctt ttgccacgca aaagcacctc
     4441 cataaatttc gattcgatgc ctctagacac agagacaaca attataagtc agactatctc
     4501 agtataacat ttgtagcgga tttttgcaag tgcgaaaaac gctttatttc aaatttgtca
     4561 atactttttc aatatctgag aagacatcat tgatatcttg attaccttcg atgtcatgaa
     4621 ccaaaccttt ggcacggtag tgagcaatga ttggttctcc ttgagcaata ttaacatcca
     4681 aacgacgttt tactgtctca ggcttatcat cttcacgttg gtagtaatct tcttctttat
     4741 agtcaactgg tgggttaaag accttgtgga aagtttctcc agttacgcgg tggatgatac
     4801 ggccactcaa acgttccaag aggctgtcag ggttcacttc aatattgata ataccttcta
     4861 gttcaatgcc aagttcagcc aatgttttgt ccaaggcatg agcttgttca attgtacgtg
     4921 ggtaaccatc caataagaat cctgtttctt taatatcatc ttgtgaaagg cgttctttta
     4981 cgattccatt tgtaacttcg tcaggaacca attcaccctt gtcaatatat gacttagcaa
     5041 gaacacccat ttcagtttga tttgccattg cagcgcggaa catatcacct gttgagatat
     5101 gtgcaacatg gaattgttct acgatttttg ctgcttgagt tcccttacct gcaccaggta
     5161 agcccataat caaaagattc atgatttgat ctccttattt tatttttaaa tagaagcaaa
     5221 aagtcttttc acccctattt aaaaacaaaa tagaagaggg gagatccaac tcttacaaat
     5281 gtcagagttt aaacctccac tccctcagta tttactgatt cagtaaatac ttttattctg
     5341 ttctgtccat gaaaccaaca tacttacgtt tcaataggta accttccaat tgcttgattc
     5401 cttcgatacc tgtagagata atgatcaaga gacttgttcc accaaaggca acaacatcag
     5461 aaagaccaaa tacatcttta gctgcaatcg gtaaaatgga aatcacacca aggaagaggg
     5521 aaccaacagt tgcaagacga cgaagaagtt tagacatata ttcttctgta cctttaccag
     5581 gacgaactcc atggatatag gcaccactct tttgtaggct ctctgctgct ttttcaggat
     5641 taatctgtac aaacgtatag aagaatgtaa agagaataat caacaaagca tacatggcaa
     5701 taccagttgg agaagtagtt gccaacatct cttgtgctac ccttacccaa gcccaatcat
     5761 gacctgtggc actcaaaaac tgaagaatag ccgcaggcgc tgcagtaatc gaactggcaa
     5821 agataacagg gataactcca gcagggttta cctttaacgg aaggtaagag ctagatggag
     5881 caccttgtgc aaccttagta tattggattg gaattttgta ttctgcttgt tgaacataag
     5941 ttgtaaagta aataatcaac aatacagtaa taatcaaaat gattacgaaa atgatagatg
     6001 aagtgatacg gctacttggg acgttcacaa agtagtccac atagatgccc tgaatcatct
     6061 ctggaattga ggaaacaatc ccggcaaaga taatcatgga aacaccgttt ccgtatccct
     6121 tatctgtaat ttgctcaccc aaccaagtga caatcatact accagctgtt aagatgatac
     6181 caatcgtcag aaaaacttgt ggagttaaag cagttttaat caattgagct ccagccaagg
     6241 tattaaaacc agctgtaatc ccgatagatt gcacaaaagc gagaactaga gcaatataac
     6301 gagtagcttg attcaatttt cttcgaccta cttccccttg tttaccccac tctacaaact
     6361 tgggtaaaat atccatttgc aagagttgga caacaataga agcggtgata tagggactaa
     6421 ctcctagggc aaaaatcgaa aagtttttta gggcattccc cgacaccaag ctcaacatgt
     6481 ttaagaagga taatccactt aaagcattca agctattggc attcacacca ggaactgtaa
     6541 tgctagttcc gatacgaaag accaaaacga taaaaattgt aaataaaatt tttgatcgaa
     6601 cctgcttgac tttaagagct tctcttaata atttaaaaaa cataggtcac ctctcttaga
     6661 tgacttctac tgaaccacct ttagcagtga tagcttcttc agctgattta gagaatttag
     6721 ctgctttcac agtcaatttc ttagtcaact caccgttacc aagaatttta atacctgact
     6781 tttcagcttt aacaattcct gcttcgataa gaacaactgg agttacttca gcaccatctt
     6841 caaagacgtt caattggtca aggttcacaa ttgcgtattc tttagcgttg atgttagtga
     6901 atccacgttt tggaagacga cggaacaatg gagtttgtcc accttcaaaa ccaaggcgaa
     6961 ctccgccacc gctacgagct ttttgacctt tttgaccacg accagatgtt ttaccgttac
     7021 ctgatgaagt accacgacca acgcggttac gtactttacg agaaccttct gcaggtttca
     7081 attcatgaag tttcattttt attttctcct cttttgtaaa atgctagcgc cgataaggga
     7141 gaaaaggttg tctcccccat caactcgcct atacgatgtc atcatagatg actatatcta
     7201 gttttagggg atggtacagt gcacatcccc taaaacttca ttagtttact tcttcaactg
     7261 ttactaagtg agatactgct gtaatcatac cacggatagc agcgttatct tctttaataa
     7321 cagagctgtt caatttgcca agtccaagtg ctacaacagt tttacgttgt gatggaatgc
     7381 gtccgattgg agacttagtc aaagtaattt taatttgagc cattttatcc cctttcttat
     7441 gccaaatcag aaactgaaat accacgaagg gcagcaattt cttcagcgcg tttcaattgt
     7501 ttcaaacctt caacagttgc acgaacaatg ttgattggag tgttagaacc aagtgattta
     7561 gatgtaatat ctgccacacc tgccaattcc acaacggcac gaactgcacc accagcggca
     7621 actccagaac cttctacagc aggtttcaac aatactttag ctccaccgaa ttctgaaaga
     7681 acttcgtgtg ggattgttgt tccaaccata ggaacttcga tcaagttttt cttagcatca
     7741 tctactgctt tacggattgc ttctggaact tcttgagctt taccagtacc aaatcctacg
     7801 cgaccattgt ggtcaccaac aacaacaaga gctgcgaaac gaagacgacg tccaccttta
     7861 acaacttttg taacacggtt gacagcaact acgcgttctt ctaattcaac tgcattgtct
     7921 ttaaatgcca ttttctagtg tcctcctatt agaatttcaa tccgttttca cgagctgcat
     7981 cagccaaagc tttcacacgt ccgtgatata gatatccacc gcggtcgaac accacttctg
     8041 aaataccttt agcgtttgca cgttctgcaa cgagtttacc gacagcaacg gcttgttcag
     8101 ttttagttcc ttttgaaact tctttatcaa gagttgaagc acttgcgagc gttacacccg
     8161 ctacgtcatc aatcacttga gcgtagatgc ctgtattaga acggaatacg ttcaaacgtg
     8221 ggcgatcagc agttccagag agttttccgc gaacgcgacg gtggcgtttt tggcggagtt
     8281 tgtttttatc tggttttgaa atcacagttt tcacctcttt agttttaaat cgtgtgctat
     8341 gcacaaagtt ggaaaatagg ttggtggttg atgatcaacc actcaacatt atttacctgt
     8401 tttaccttct ttacggcgaa cgaattcacc aacgtaacgg atacctttac ctttatatgg
     8461 ttctggtgaa cgaaggctac gtacgtaagc agctgtttga ccaactactt cttttgaaat
     8521 tccgctaaca acgattgttg ttgggtttgg aagttcaaaa gtaattcctt ctggagcttc
     8581 aacttcgtct ggatgagatt taccaacagc caaaacaagt ttagatcctt gaagctgtgc
     8641 acggtaacca accccacgca tttcaagttc tttcttgaat ccttctgata caccaacaac
     8701 catgttgttc aaaagggcac gagtagttcc gtggatagtt ttcatttctt ttgaatcgtt
     8761 tggacggtga agagttattt cagtaccttc cacacggatt tcaatatctt ttgagaactc
     8821 acgagtaagt tctcctttag gtccttttac agttacaacg ttgtcattgt tagcgagttc
     8881 aacaccagca ggcaacacga taactttatt accaatacgt gacatgttta ttttctcctg
     8941 ttaaattgtc aggccagaac ggccagtttt cacggggttc agatacttat ttagttcaag
     9001 agctgaactg aacacgctct aagaactgtg caaaatagat aagttggctt gtttgtaaac
     9061 aaacttctcc aacttcctaa ttttgctttg ttctttacga gctttgtatc ttgattttac
     9121 caaacgtaag cgataacctc accaccaaca ttcttttggc gtgcttcttt atcagtaagc
     9181 aaaccttcag aagttgaaag gatggcaatt ccaagtccgt taagaacttt tggaaggtct
     9241 tcacgttttt tgtagacacg aagtcctggt ttagaaacac gtttcaagtt agtgataact
     9301 ttttcaccat ttggtccgta tttaagaaat acacggatga cgccttgttt gtcatcttcg
     9361 atgatttcaa cgttttttac aaaaccttcg cgtttaagga tttcagcaat cccttttttg
     9421 atgtttgatg caggtacttc aagtacttcg tgtttagctt ggttagcatt acgaatacga
     9481 gttaggaagt ctgcgattgg gtcagtcata accattttgt ttttctcctc ttactagtag
     9541 tttgcaagtt gcacttgcta gttaatacat gatacaaaga gcgtaacagc acgagcaaaa
     9601 atagacaatt taatgcagaa gcaatgctcc aaagcaaatt ggtctttttt gccaaagctg
     9661 tagctcgggc taaactctgt gtgaaaaaga taaactttcc tagaaacttt agtttcttcg
     9721 tcaagtttcc tatttttact tggagttttg acacccttga tatcttaaat taccaagatg
     9781 cttttgttac accaggaatt tgacctttat gcgcaagttc gcgaaaagcg atacgactca
     9841 gaccaaattt gcggtaaact gaatgtgggc gccccgtaac tctacaacga ttatgtaaac
     9901 gagtcggtga ggcgttgcga ggtaatttag ataaaccttc gtagtcccct gccgccttta
     9961 atgcagcacg tttttcagca taacggtcaa caattttttg gcgtttagcc tctctagcta
    10021 ccattgattt tttagccatt agatttacct cctatattat tttgcaaaag gcattccaag
    10081 gcctgtaagc aatgcacgtg actcttcgtc agtgttagca gttgttacga taacgatgtc
    10141 aagaccacga gttttgtcaa cgtcatcgaa gttgatttct gggaagatta attgttcttt
    10201 cacaccaagt gtgtagttcc cgcgtccatc aaatgatttt gttggaacac cgtgaaagtc
    10261 acgtacacgt ggaagtgaaa ctgataccaa tttatccaag aattcgtaca tacgttcacc
    10321 acgaagggta acttttgcac cgatcgcaac accttcacga agacggaagc cggcgattga
    10381 ttttttagct ttagtgataa gtggtttttg acctgagata agtgccaatt cttcagcagc
    10441 tttttcaagg cttttagcgt ttgatacagc ttcaccaaca cccatgttca aaacaatctt
    10501 atctacttta ggcacagcca tcactgatga gtagttgaat tgttctgtca aagcaggaac
    10561 tacttcatta agatattttt cttttaaacg atttgccatt atacttctcc tttccttcgt
    10621 gattaatcaa gcacttcgcc tgattttttg ttgtagcgaa cttttttacc gtctacaaat
    10681 ttgtaaccaa cacgaccagc tacaccattt ttgtccaaaa cttgaacgtt tgatacgtgg
    10741 atagctgctt ctttctcgat gataccacct tgaggaagct cgttagttgg acgttggtgt
    10801 ttcttaacaa tgttaacacc ttcaacgata actttgttta cttttggaag ggcagtaagg
    10861 acaacagctt ctgttccctt atctttacca gcgattacgc gaactttgtc gccttttttt
    10921 acaaacatta ggtttctcct tgatttttct tacgcccata agggcaccct agcttgaagc
    10981 taggggacta gtttgttcct aaaaattaaa gtacttctgg agcaagtgac acgatcttca
    11041 tgaagccacc ttcacgcaat tcacgtgcaa ctgggccaaa gatacgtgtt ccgcgaggag
    11101 ttttgtcttc acggatgata actgctgcgt tttcgtcaaa tttgatgtat gaaccatcag
    11161 cacgacgagc acctgattta gtacgaacga taactgcttt aacaacgtca ccttttttaa
    11221 ccgcaccacc aggagtagct tgttttacag atgccacgat aacatcaccg atgtttgcaa
    11281 atttacgtcc tgaaccacca agaactttga tagtcaagat ttcgcgagca ccgctgttgt
    11341 ctgcgacttt caaacgagtt tctgtttgaa tcatttcagt tttctccttt caggtttgat
    11401 tagatgatga ccgcttcttc aacaacttct acaagacgga aacgttttgt agctgaaagc
    11461 gggcgagttt ccatgatacg tacgatatcg ccttctttgg caacattgtt ttcatcatga
    11521 gctttgtatt ttttagagta gttaatacgt ttaccataga ctgggtggtt acgttttgtt
    11581 tcaactacaa ctgtgattgt cttgtccatt ttgtcagata caacacgtcc aacaagaact
    11641 ttacgattat tgcgttccat tgaaatttct ccttccctag tctattattt cgcttcagat
    11701 tgaactgttt tgatacgagc gatttgtttt ttaacttctt tcaagcgagc tgtttgttcc
    11761 aattgaccag tagcagcttg gaaacgaagt tcaaacaatt cttttttcaa ttcgttttcg
    11821 cgcttcgcga gttcttcttg agaaagacca cgaagttctt taacaaattc ttttacttca
    11881 ttaagtttca tgccttctcc ttattctgct tcacgtttta cgaatttaca tttaactggc
    11941 aatttgtggc tagcaagtcg aagcgcttca cgtgcaatct cttcagatac accagcgatt
    12001 tcgaacatca ctttaccacg tttaactggt gctacccaac cttcaggtgc ccctttacca
    12061 gatcccatac gcacaccgat agctttagca gtgtatgatt tgtgtgggaa gattttaatc
    12121 caaactttac caccacgttt catgtaacga gtcatggcga tacgagcagc ttcgatttgg
    12181 cggttagtga tccagtggct agttgtagct tgaagaccgt attcaccgaa tgctacttct
    12241 tttccacctt ttgcttcacc gcgcattttt ccacggaact cacgacggtg tttaacacgt
    12301 ttaggtacta acattggtta tttacctcct ttagtgtttt tacgagctgg aagaacttca
    12361 ccacggtaga tccatacttt aacaccaagt ttaccgtatg tagtatctgc ttcttcccaa
    12421 gcgtaatcga tatctgcacg aagtgtgtga agcggaacag ttccttcaga gtatccttca
    12481 gcacgggcga tatctgcacc gttcaaacga cctgatactt gagttttgat tcctttagct
    12541 ccagcacgca ttgcacgttg gattgcttgt ttttgtgcac gacggaaagc aacacgttgc
    12601 tccaattgac gagcaattcc ttcacctaca aggtgagcat ccaaatcagg ttgtttgatt
    12661 tcgatgatgt tgatgtgtac ttgttttcca gtcaatttgt taagttttgc acggagtgca
    12721 tcaacgttag caccaccttt accgataacc atacctggtt tagcagtgtg aagtgaaacg
    12781 ttaactttgt ttactgcgcg ttcgatttca atagttgaaa ctgctgcgtc agcaagttct
    12841 ttttgaacga atttacggat tgcaagatct tcatgaaggt aatccgcgta ttctttttca
    12901 gcataccatt tggcatccca atcacggatg atgccgacac gcataccaat tggatgtact
    12961 ttttgaccca cgattttacc tccttatttt tctgcaacag ctacagtgat gtgagctgta
    13021 cgtttgttga ttggtgaagc tgaacctttc gcacgtggac ggaaacgttt catagttggt
    13081 ccttcgtttg cgaatgcttc agatactacc aagttagctt tatccaaacc aaagttgttt
    13141 tcagcgttag ctacagctga gttcaaaact ttcaagatga tttcagcagc tttgtttgga
    13201 gtgaatgtca agattgcgat tgcatcggct acgcttttac cacggatgtt atcaagaaca
    13261 agacgtgatt tacgaggtga aacacgtact gtacgagcca ttgctttagc tgaagtaatt
    13321 tctgccattt atgttctcct tattttctac gtgttttctt gtcgtctgca gcgtgacctt
    13381 tgtaagtacg agttggtgca aattcaccaa gtttgtggcc taccatgtct tcttggatgt
    13441 aaacaggtac gtgtttacgt ccgtcataaa ctgcaatagt gtaaccaatg aaacttggga
    13501 agatcgttga tcgacgtgac caagttttaa taactttttt cttttcgtcg ttagcttgag
    13561 cttcaacttt tttcatcaaa tgctcatcga cgaaaggtcc ttttttaaga ctgcgtccca
    13621 tttttatatt ttctccttta aatgttgtac cacagcggct tgcactccta tggagcgcta
    13681 ccgagctggc ggatttacta gttgcttaag cgactagttt aatattattt ctcgttgcga
    13741 cgacgaacga taagtttgtc agatttcgct ttcttgttac gagttttaag accaagagca
    13801 ggtttgcccc atggagtaga tggtgcttta cgaccaactg gtgctttacc ttcaccacca
    13861 ccgtgtgggt gatcgttagg gttcattaca gaaccacgaa ctgttgggcg gatacctttc
    13921 caacggctac gtcctgcttt accaaggttt acaagtccat gttgttcgtt tccgacaaca
    13981 ccaactgtag cacggcaagt tccaagaatc atacgaactt cacctgattg aagacgaaca
    14041 agaacgtatt taccttcaga acccaatact tgagcagatg caccagcagc acgtaccaat
    14101 tcaccaccac gacctggttt caactcgatg ttgtgaatca aagtaccaac tgggatgtta
    14161 gcaagtggaa gagcgtttcc gactttgata tctgcttctg gacctgaaac gatacgttga
    14221 cctacttcaa gaccttttgg agcgatgatg tatgctttca caccgtcagt gtagtgtaca
    14281 agagcgatgt ttgcagaacg gtttggatcg tactcgattg ttttaacaac tgcttcaacg
    14341 ttgtctttat tacgtttgaa gtcaaccaaa cggtagaaac gtttgtgtcc accaccttgg
    14401 tgacgaactg tgatacgacc gttgttgtta cgaccagcct tgctcttcaa tgcaacaagc
    14461 aatgattttt caggagtgct tgttgtgatt tcagcgaaat ccaaagaagt catattacgg
    14521 cgaccgtttg ttgttggttt ataaacacga attcccacga tatttcctcc ttagattatt
    14581 cagcttcagc agcaaacaac tcgattgctt tagaatcagc tgtaagtgtg atgatagctt
    14641 ttttagtttt gttagtaaaa ccagtgtaac gtccaacacg tttagctttt ggttttacgt
    14701 tgattgtgtt aacattggca actttaacac cttcgaaagc agcttcaaca gcttgcttga
    14761 tcaaaagttt gtgtgcacga gtgtcaactt caaatacata tttccctgct tcaagttgag
    14821 ccattgagct ttcagtgatg acaggttttt tgataacatc atacaaattc attatgcaag
    14881 aacctcctcg attttagaga tagctgcttg tgtgacaaga agtttgtcgc tatttgcgat
    14941 gtcaagaaca cttgcagttg tagcagttgc aactttcacg tttggaaggt tacgagctga
    15001 aagagctgcg aattcatttc cttcttcaag gataacaaga actttagaat cgatgctcaa
    15061 tgctgcaaga acttttgcaa attcagcagt ttttggagct gtaaatgaaa gagcgtctac
    15121 agctacgaat ttgttttcag caactttttc agagtaaact gatttaagag ctaggcgacg
    15181 aactttttgt ggaagtttgt agccgtatga acgtggagtt ggtccgaaga caacaccacc
    15241 accacgccat tgtggtgagc ggatagaacc ttgacgagca cgtccagttc ctttttgacg
    15301 ccacggtttg cgtccaccac ctgatactgc agagcggttt ttaacagcgt gtgttccttg
    15361 acgaaggctt gcgcgttggc tgatgattac atcaaacaca actgattcat ttggttcgat
    15421 accaaatact gcatcgttaa gaacaacttg gccagcttct ttaccagttt ggtcaaataa
    15481 tgttacgttt gccattgtga ctgatttccc ctttctttat tatttaccag ctttaactgc
    15541 tgatttgata gtgataagag atttcttagc acctggtacg ttacctttga taaggataac
    15601 gttcttttct ggaacaactt gtacaacttc aagattttga attgttacgc ggtcaccacc
    15661 catacgtcct gcaaggtttt tacctttgaa tacgcggtta ggtgcaacag gtcccataga
    15721 acctggacga cggtggtaac gagaaccgtg agccatcggt ccacgtgatt gtccgtggcg
    15781 cttgataaca ccttggaaac ctttaccttt agaagtacca gttacatcaa caacatctcc
    15841 agctgcgaat gtttcaactg taatttcagc accaacttcc aagccttcaa cgtttttgaa
    15901 ttcacgaatg aagcgcttag gagccgtgtt agctttcgct acatgtcctt tagcaggttt
    15961 gttgctcaat acttcgcgtt tgtcatcgaa accaacttgg atagcgttgt atccgtctgt
    16021 ttcaacagtt ttaacttgaa gaacaacgtt tggagttgct tcaataactg ttacagggat
    16081 caattcgcca gcttcagtga agatttgagt cattcccact tttttcccta agattccttt
    16141 tgtcatgaga aaatagttcc ttttctatat tttttattca aaaagttttt aacgagcgtt
    16201 ttttatgctc aagtctaaga tacaaagggc gtcaaactca aagtgaaaat aggaaactaa
    16261 cgcagtgtct agcggacaat aggaagttta tctttttcac accgagtcta gcccgtgttc
    16321 aattagattg aaacaccagc ctctgctctt ttatatttta gattaaagtt tgatttctac
    16381 gtttacacca cttggaagat ccaatttcat caaggcatca actgtttttt gagttgggtt
    16441 aacgatatcg atcaaacgtt tgtgtgtacg catttcaaat tgttcgcgag agtctttgta
    16501 tttgtgagtc gcacgaatga ttgtgtagag gctacgttca gttggaagtg ggattggacc
    16561 cgcaacttgt gcacctgtac gagtagctga ttctacgatt tttgcagccg ctgtgtcaag
    16621 cgtacggtgt tcgtaagctt tcaaacggat acggattttt ttgtttgcca tctttttctc
    16681 cttttcgtct atttaagata ataggctagc tccacaagaa aaccgacgcg gtgttgcgtg
    16741 gcaatgcaac cgagcgtgtc gcaacctctt gcatcaaaac taaggctgta atttacagca
    16801 ccataataga ataacacaaa tcccctgcga ttgcaaggga tttgttaggt tttttcgttt
    16861 ttttgggatg aaactgtttt cctacttttt ctttctaaaa ttagggattt tcttctatat
    16921 ttcttctata cataagttaa gtcctcttga catcaaattt atcttctgat tggtccacta
    16981 agatatccgc cttagactca gtttctttat agtatcgcag atactgctcc cgtctcatct
    17041 gatggctagc caatataaag gatgcatcgc gatttctcac agtcgtatct ctagcaaggc
    17101 gtcgctttaa ttcggtctcc tcatccgtgt agaaacagat ggttttttca aagagttcct
    17161 tgggtagaaa accaacagac atcccttcga caatcaaaat tggtttggct ccagacaaga
    17221 cctcactagc cttccaaggt tcttcaattg tcaagacatc catacccgcc tgcaaggcaa
    17281 ggatatctct ctgcaaactc tccagttcat gcgccactgg cagactggct gtcacctttt
    17341 gattcggcgc gtccttgggt actaccaaat agcgtccgga ggtgatatag ggatctgttt
    17401 ctagcaaatt tactgtagta gaatctaaag cttggtacaa ttcctgtgca aaggttgatt
    17461 tacctgaagc tccatgaccg tatattccca gtgtcctgac tttccccgtc tcgatctctg
    17521 agactagttg gtctactaag tcttttttct tcattctctc ttcacctgtt catagctttc
    17581 ttttccgtca ttgagcttgt cccaaatcac tacttgccca cttttgagcg atttttgcac
    17641 atcgataatt cgttggttag atgaacctcg aaactggagc ataagatttc tcttagttcg
    17701 atcatatctt ccatcgacaa gaatgtcaat cagtgacaag aattccagtt tatctggagt
    17761 ttccaacatc atttcttccc aagtgtagcc ggtccaggac cagatgtcct tgtctggcaa
    17821 ttccttccga atccgcttaa caagtggcaa gagaatccca gtattaagaa aaggctcccc
    17881 tcccaacaaa gtcaaacctt gaacataggg ctgagcaaga tctgccatga tctgctcttc
    17941 taattctgct gtatagggaa tgccagcatt aaaagaccaa gtcgcaacat tataacatcc
    18001 ctcgcagtga aacatacagc ctgatacata gagagagtta cgcacgcctt cgccgtccac
    18061 aaagttaaag gccttgtagt caatgatacg accttgacta agttcctcgc ttttccattc
    18121 ttgtggtttt ggattattca ttcgctacct ctatccaata acgctcgact ccattgtgag
    18181 catcctcaaa tattccacca tttgctagaa tgactgctct gctagcagga ttattcacgc
    18241 tacaggtcac cagagctttc ttgatgttct tttccttagc aacttgcaag ccctgacgga
    18301 gagtctcttt tgcataaccc ttgcctcttt cagatggacg gatggagtag ccaatgtgcc
    18361 caccttcttc tagtaaaaag ttactgaggc gcaaccggag attaagaaat ccaactgctt
    18421 gacctttctc agaaaaagcc actaactgaa ttgcaggaac ccatccttca ggcagattaa
    18481 tccccatttc ctgttcctga ttgctttcca accactcttc atacacaaaa ttctctgtat
    18541 cccaaaatcc tccatcatgg gctgattggc tcttttcaaa ctctgtcatc atatctaaaa
    18601 cagctttctt atccgctaat cttggtctgc gtaattccat ccctacctcc cactatgaga
    18661 aaagcgagag ctggctctca cttttatttt ttcttgttct tgtttaaaaa ttgttctctg
    18721 aagctaagca agcgttcttt cttaccagct ccacctttag agtgcttctc gtgataacgg
    18781 gtcacttgtg cgcgcccctt atcgtctagt tgatattttc ccatttcatt tctttctaat
    18841 ttgttacttg atgcccagct attttaatcg ttgaaccatt catatgtttg acacgcgcag
    18901 cgatttcctt gtgacgcccg ttaaccattg gtctcgcttg aggattgcca agatagccac
    18961 aagtccgttt aacaacgtct actgttttag gatcgctatt gccacagttt ggacaagcaa
    19021 accctctctc agttggttca aaatcccctt caaagtcaca cttgtagcaa cggtcaatcg
    19081 gagtattggt gcctagatag cctacacggt cataagcata atcccagaca gcttccaagg
    19141 cctttggatt ttgctgaagg actggatact cacaataatg gatgaaacca cctgacgcac
    19201 ctgcttctgg atagactttc tcaaagtcca atttttcaaa cggtgttgga tttttacgaa
    19261 catcgtagtg gaaagagttg gtgtagtatt ccttgtctgt gatatcagga atagagccaa
    19321 acttgtctat atctagtcgg cagaaacggt ctgtcagact ttcggatggt gttgagtaga
    19381 tagagaaatg gtagccatat tggtctgacc actcttctac acggcgtttc atatcgtgaa
    19441 tgatgtctag cgtgaattcc ttagcatctg gattactttc ccagctgtta ccaaagaaaa
    19501 ctgtcgctac ttcatacaag ccgatatagc ccagcgaaac ggtcgcacga cgattcttaa
    19561 agagctggtc aacactttct tctttaccta gacgatggcc aaaagcaccg tactgataaa
    19621 gaataggagc attcgctggt gtcgcctctt tagtgcgttc gacacggtaa acaagagcat
    19681 cttctgcgat attcattcgc tcgttgaaga tttcccagaa cttattcata tcaccttcag
    19741 actcaagagc aatacgaggc agattaaccg tcacaacacc cagattcatg cgacctgaat
    19801 tgacttctac accattttca tccttccacc cttgaaggaa agaacggcag cccataggaa
    19861 ctttgaaaga acctgtcaaa tcaacaatct tatcataaga caagacgtct ggatacatcc
    19921 gcttggttgc acactctaga gccaactgct tgatgtcata gttgggagtt ccttcctcta
    19981 agttgaggcc tcttttaagc gtaaagataa gtttaggaaa gatagccgta cggtgttctg
    20041 aaccaagacc cttgatgcga atgtttaaaa tagctttttg aatttctcgt tcaaaacgac
    20101 tggttcccag accaaaacct aacgaagtaa aaggtgtttg tccatttgaa gtgaagagag
    20161 tattgatttc atactcaaga gattgcatgg catcgtagat gtccttttgc gttttcttcc
    20221 aagcgtaatc ttcccgtttt tcaggcaata cccactcttc tgcatctttg agatgttttt
    20281 gatagttctt ctctgcataa ggcgccaaaa tttcatcgat acggtcagct gaacagccac
    20341 cgtactggct agaagcaacg ttggcaatga tttgagaaat ctgtgctgtc gcagtctgga
    20401 tagacttggg actctctacc tctgcatttc caatcttaaa accattttcc aacataccct
    20461 taaaatcaat caaacagcag ttggtcatag gggtataggg actgtagtcc aaatcgtgat
    20521 agtggatatc ccccttttgg tgggcattgg ctacgtgctt aggaagcatt tgcagtccga
    20581 ttgatttccc aacaatccct gctgtcaaat cacgctgagt gttaaagaca tcactgtctt
    20641 tattagcatt ttcattgaca actgtctggt ctttgttgag aagtttatga atactaaagt
    20701 tgatatccgt cgcttttgag cgctcaaaat ccctctgtgt ccgataagta atatactcct
    20761 cagccagcgc atattctttg gcttcaagga gttcatgttc tacgatattt tgaatttcgt
    20821 aaatcttaat tccctgtgga aagcgactat gaatttctgt aataattcgc tcagtcagat
    20881 cattgaggcg tttttcaacc aggggtgtca catccataac cttgtcagcc gccttgtgga
    20941 gagccttgtc aatcttgtcc acatcaaata caacacgtct cccatctcgt ttctcgacga
    21001 agagagttgg caaaattgta attttttctt ctagtgcaat catacgcatg ctcttttcta
    21061 aaatgttctt tctaa
//
LOCUS       DAVMGZ010000023        20534 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000023 DAVMGZ010000000
VERSION     DAVMGZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.024"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            71..940
                     /locus_tag="VYQ93_000710"
     CDS             71..940
                     /locus_tag="VYQ93_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV3335441.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            944..1900
                     /locus_tag="VYQ93_000711"
     CDS             944..1900
                     /locus_tag="VYQ93_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV3335442.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1900..2664
                     /locus_tag="VYQ93_000712"
     CDS             1900..2664
                     /locus_tag="VYQ93_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3335443.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2664..3374
                     /locus_tag="VYQ93_000713"
     CDS             2664..3374
                     /locus_tag="VYQ93_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3335444.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3788..4444
                     /locus_tag="VYQ93_000714"
     CDS             3788..4444
                     /locus_tag="VYQ93_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV3335445.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            4552..5647
                     /gene="prfB"
                     /locus_tag="VYQ93_000715"
     CDS             join(4552..4623,4625..5647)
                     /gene="prfB"
                     /locus_tag="VYQ93_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV3335446.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5665..6357
                     /gene="ftsE"
                     /locus_tag="VYQ93_000716"
     CDS             5665..6357
                     /gene="ftsE"
                     /locus_tag="VYQ93_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV3335447.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6350..7276
                     /locus_tag="VYQ93_000717"
     CDS             6350..7276
                     /locus_tag="VYQ93_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3335448.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVETNTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
     gene            7562..9742
                     /locus_tag="VYQ93_000718"
     CDS             7562..9742
                     /locus_tag="VYQ93_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV3335449.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            9794..10609
                     /locus_tag="VYQ93_000719"
     CDS             9794..10609
                     /locus_tag="VYQ93_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV3335450.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            10749..12092
                     /locus_tag="VYQ93_000720"
     CDS             10749..12092
                     /locus_tag="VYQ93_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3335451.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            12307..13497
                     /locus_tag="VYQ93_000721"
     CDS             12307..13497
                     /locus_tag="VYQ93_000721"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV3335452.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            14050..14985
                     /locus_tag="VYQ93_000722"
     CDS             14050..14985
                     /locus_tag="VYQ93_000722"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV3335453.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFTYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            15019..16056
                     /locus_tag="VYQ93_000723"
     CDS             15019..16056
                     /locus_tag="VYQ93_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV3335454.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            16197..16802
                     /locus_tag="VYQ93_000724"
     CDS             16197..16802
                     /locus_tag="VYQ93_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV3335455.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            16960..17490
                     /locus_tag="VYQ93_000725"
     CDS             16960..17490
                     /locus_tag="VYQ93_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV3335456.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            17511..18413
                     /locus_tag="VYQ93_000726"
     CDS             17511..18413
                     /locus_tag="VYQ93_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HEV3335457.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGTDID
                     AACGQLRSNTMKRDRQKAVAAVNP"
     gene            18413..18895
                     /locus_tag="VYQ93_000727"
     CDS             18413..18895
                     /locus_tag="VYQ93_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV3335458.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            18897..20438
                     /locus_tag="VYQ93_000728"
     CDS             18897..20438
                     /locus_tag="VYQ93_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV3335459.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
BASE COUNT         6138 a         3714 c         4550 g         6132 t
ORIGIN      
        1 cattgagtat aagaacctat caaaaagtga gggaaacccc tcggaattat aaatagaaag
       61 agtgaatctt atgctccaac aactcgtaaa tggtttgatt ctaggtagtg tttacgcgct
      121 gttagcccta ggatatacca tggtttacgg aattatcaag ctcatcaact tcgcccatgg
      181 tgatatttat atgatgggag cctttatcgg ttatttcttg atcaattctt tccaaatgaa
      241 tttctttgta gcgcttattg tagctatgct agcgacagct attcttggtg tcgtgattga
      301 gtttcttgct taccgacctt tgcgccactc tactcgtatt gctgttttga ttacggctat
      361 tggggtttct ttcctattgg agtatggaat ggtctatctg gttggtgcca atacccgtgc
      421 cttccctcaa gcgattcaaa cagttcgata tgatttggga ccaattagct taacaaatgt
      481 gcagttaatg attttggcta tttccttgat tttgatgatt ttgttacaag tcattgtcca
      541 aaagactaag atggggaaag ccatgcgtgc agtatcagta gatagcgacg cggcgcaatt
      601 gatggggatc aatgtaaacc gtacgattag ctttaccttc gctttgggtt ctgctcttgc
      661 gggtgcggct ggtgttctga ttgctcttta ttataactct cttgagcctt tgatgggggt
      721 tactccaggt cttaaatctt tcgttgccgc agtacttggt ggtatcggaa ttattcctgg
      781 tgcggctctt ggtggctttg tgattggtct attggaaacc tttgcgactg cctttgggat
      841 gtcagatttc cgtgatgcca ttgtttatgg aatcttgttg ttgatcttga ttgtccgccc
      901 agctggtatc cttggtaaga atgtgaaaga gaaggtgtaa acgatgaagg aaaatttaaa
      961 agttaatatt ctatggttac tccttttgtt agctggctat agcttgatta gtgtactggt
     1021 ttcagtcgga gtacttaatc tattctatgt acagatttta cagcaaattg gaattaatat
     1081 tattttggct gttggtctca acttaatcgt tggtttttca ggacaatttt cacttggtca
     1141 tgctggtttc atggcgattg gtgcctatgc agcagctatt attggttcta aattaccaac
     1201 ctacggtgcc ttctttggag ctatgcttgt aggggctttg ctttcaggag cagttgcctt
     1261 acttgtcggc attccaacct tgcgcttgaa gggggactat cttgcggtag caactctggg
     1321 tgtttctgaa attatccgta tctttatcat caatggtgga agccttacaa atggtgcggc
     1381 aggtatctta gggattccta actttacaac ttggcaaatg gtttacttct ttgtcgtgat
     1441 tacaaccatt gcaaccttga acttcttgcg tagcccaatt ggtcgttcaa ccctctctgt
     1501 tcgtgaagat gaaatcgctg ctgagtcagt tggggttaat acgactaaaa ttaaaatcat
     1561 cgcttttgtc tttggtgcca ttactgcaag tattgctggg tcacttcagg caggatttat
     1621 cgggtctgtt gtaccgaaag attacacctt catcaactca atcaacgttt tgattattgt
     1681 tgtatttggt ggactcggtt ccattacagg tgcgattgtt tcggctattg ttctgggaat
     1741 tttgaatatg cttctccaag atgttgctag tgtgcgtatg attatttacg ctttggcctt
     1801 ggtattggta atgattttca gaccaggtgg actccttgga acatgggaac tgagcctatc
     1861 acgtttcttt aaaaaatcta agaaggagga acaaaactaa tggcattact tgaagtaaaa
     1921 cagttaacca aacattttgg tggtctaaca gctgttggag atgtgactct tgaattgaac
     1981 gaaggggaac tggttggatt aatcggtcca aacggagctg ggaaaaccac ccttttcaac
     2041 cttttgaccg gtgtttatga accaagcgag ggaacagtaa ccctagatgg tcaccttttg
     2101 aatgggaaat caccttataa gattgcctct ttgggacttg gacgtacttt ccaaaatatc
     2161 cgtctcttta aagatttaac agttttagac aatgttttga ttgcttttgg aaaccatcac
     2221 aaacaacatg tttttactag tttcttacgc ttaccagctt tttacaagag tgaaaaagaa
     2281 ttaaaggcta aagctttgga attgttgaaa atctttgatt tagatggtga tgcagagact
     2341 cttgctaaaa atctttccta cggacaacaa cgtcgtttgg aaattgttcg tgcccttgct
     2401 acggaaccta aaattctctt cttagatgaa ccagcagcag gtatgaaccc acaggaaaca
     2461 gccgaattga ctgagttaat tcgtcgtatc aaagatgaat ttaagattac aatcatgttg
     2521 attgaacacg atatgaatct ggtcatggaa gtaacagaac gtatctacgt acttgaatat
     2581 ggccgtttaa ttgctcaagg aactccagac gaaattaaga ccaataaacg cgttatcgaa
     2641 gcttatctag gaggtgaagc ctaatgtcta tgttaaaagt tgaaaatctt tctgtgcatt
     2701 acggtatgat ccaagcagtc cgtgatgtaa gctttgaagt taatgaagga gaagttgttt
     2761 cccttatcgg tgccaacggt gcaggtaaga caactattct tcgcacattg tcaggtttgg
     2821 ttcgaccaag ttcaggaaag attgaatttt taggtcaaga aatccaaaaa atgccagctc
     2881 agaaaattgt ggcaggtggt ctttcacaag ttccagaagg acgccacgtc tttcctggct
     2941 tgactgttat ggaaaatctt gaaatgggag ctttcttaaa gaaaaatcgt gaagaaaatc
     3001 aagctaactt gaagaaggtt ttctcacgct ttcctcgtct tgaagaacgt aagaaccaag
     3061 atgcagctac tctttcagga ggggaacaac aaatgcttgc catgggacgc gctcttatgt
     3121 caacaccaaa acttcttctt ttagatgaac catcaatggg acttgcccca atcttcatcc
     3181 aagagatttt tgatatcatt caagatattc agaagcaagg aacaaccgtc ctcttgattg
     3241 aacaaaatgc caataaagca cttgcaatct ctgaccgagg atatgtactg gaaacaggga
     3301 aaatcgtcct atcaggaaca ggaaaagaac tcgcctcatc agaagaagtc agaaaagcat
     3361 atctaggtgg ctaaaacaat ccagtggatt gttttagtcg gcagatgaag attacgaagt
     3421 aatcatcatt atagtctggg agactagttt aggttgcaga taaagattgc gaagcaatca
     3481 tcaaatccag tggattgttt tagtcggcag atggagatta cgaagtaatc atcaatatag
     3541 tccgggggac ctttttagtc ggtagattga gattgcaaac aaatctgcat ctacattgaa
     3601 agcttaattt ctaataattg aaaaaatcga atgaaaaatt tcttaccttc attcacagag
     3661 ctcgatttca gagctctttt tgctagctta ttcatacttt tctgaatttc gaaaaagaaa
     3721 tgtaagcgtt tgatagattt acaaaaagat tgtataatag ggataagaat agaaaaggag
     3781 aagtctcatg gcagttaaag attttatgac ccgcaaggta gtttatatta gtccagatac
     3841 aacagtatct catgcagcag atttgatgag agagcaaggt ttgcaccgtc tgcctgttat
     3901 cgaaaatgat caattagttg gtttggtgac tgagggaacc attgcacaag caagtccatc
     3961 taaagcaaca agtctttcta tctatgagat gaattatctt ctgaataaga caaaagtaaa
     4021 agatgtcatg attcgcgatg ttgtcactgt ctcaggctat gctagtctag aagatgcaac
     4081 ttatctgatg ttgaaaaata agattagtat tctccctgtc gtagataacc atcaagtata
     4141 cggagttatt actgaccgtg acgttttcca agcctttctt gaaattgcag gttatggcga
     4201 agaagggatt cgtgtacgct ttgttacaga agatgaagtt ggtgttcttg gaaaaattgt
     4261 ttctttgatt gtagaagaaa atttgaatat ctcccataca gtcaatattc cgcgtaagga
     4321 tggtaaggtg attatcgaag tgcaaatcga tggatcaatt gatttaccag ccttgaaaga
     4381 aaaatttgaa gcaaatggta ttcaagtgga agaaatcgct cgcacttcag caaaagtctt
     4441 gtaagaaggg aagcccaaag gcttcttttt tcatgaaaag gggattagag aaaaagatgg
     4501 aaagaaatga taaaatatgc tataatgaaa taatgtaaaa aaggagtatt tatggacatt
     4561 tcagtaattc gtcagaaaat tgacgcaaat cgtgaaaaat tagcttcttt cagggggtct
     4621 ctttgacctc gaagggctag aggaagagat tgccatcttg gaaaacaaga tgacagaacc
     4681 tgatttttgg aacgataata ttgcagccca aaaaacgtcg caagaattaa atgaattaaa
     4741 aaacacttac aataccttcc ataagatgga agagttgcag gatgaagtcg aaattttatt
     4801 ggattttttg gctgaagacg agtcagtgca tgatgaactg gtagcgcagt tagccgaact
     4861 tgataagata atgaccagct acgagatgac tctactcttg tcagaacctt atgaccacaa
     4921 caatgccatc ttggaaatcc atccaggttc tggtggtact gaggcgcagg actggggtga
     4981 tatgttgctt cgtatgtata ctcgttatgg taatgctaaa ggctttaaag tggaagtgtt
     5041 ggattaccaa gcaggtgatg aggctggtat taagtcggta actttatcat ttgaagggcc
     5101 taatgcctat ggtctcctca agtcagaaat gggtgttcac cgcttagtgc gaatctcacc
     5161 atttgactct gccaaacgtc gccatacctc tttcacatct gtagaagtga tgccagaatt
     5221 ggatgatact attgaagtgg aaatccgtga agatgatatc aagatggata ccttccgttc
     5281 aggtggtgcc ggtggacaaa acgtcaataa ggtttcaaca ggtgtacgtt taacccacat
     5341 tccaactgga attgttgtcc aatcaacagt ggatcgtacc cagtatggaa atagagatcg
     5401 tgccatgaag atgttgcagg ctaagctcta tcaaatggag caagagaaga aggctgcaga
     5461 ggtagattct ctcaaaggtg agaaaaagga gatcacttgg ggaagccaaa tccgttctta
     5521 tgtcttcacg ccttatacta tggtaaaaga tcaccgaact agctttgagg ttgctcaggt
     5581 agataaggtt atggatgggg acttagatgg ttttatcgat gcttatctca agtggcgaat
     5641 tagctaagat agaaaggaac tcacatgtca attattgaaa tgagagatgt cgttaaaaaa
     5701 tacgacaacg gaacgactgc tctacgcggt gtttcggtta gcgttcaacc gggggaattt
     5761 gcttacatcg taggaccttc aggagcaggg aagtcaactt ttattcgttc tctgtatcgt
     5821 gaagtaaaaa tcgataaagg aagcctatca gttgctggtt ttaatctggt taagatcaaa
     5881 aagaaagata tcccgcttct acgtcgtagt gttggggttg tcttccagga ttataaattg
     5941 ttaccaaaga aaactgtcta tgaaaatatt gcttacgcta tggaagtaat cggggaaaat
     6001 cgccgtaata tcaaaagacg agtgatggaa gttttggact tggttggatt gaagcataag
     6061 gttcgttctt tcccaaatga actctcaggt ggggagcaac agcggattgc gattgcgcgt
     6121 gcaattgtaa ataatcccaa agtattgata gctgatgagc caacaggaaa tctggatccg
     6181 gataattcat gggaaattat gaatcttttg gaacggatta acctacaagg aacaactatt
     6241 ttgatggcga ctcataatag ccagattgta aataccttgc gccaccgtgt cattgccatt
     6301 gaaaatggcc gtgtcgttcg tgacgaatca aaaggagagt atggatacga tgattagtag
     6361 attttttcgc catttatttg aagccttaaa aagtttgaaa cgaaatggtt ggatgacagt
     6421 agctgctgtc agttcagtca tgattacttt gaccttggtg gcaatatttg catctgttat
     6481 tttcaataca gcgaaactag ctacagatat tgaaaataat gtccgtgtag tagtttatat
     6541 ccgaaaggat gtggaagata atagtcagac aattgaaaaa gaaggtcaaa ctgttacaaa
     6601 taatgactac cacaaggtat atgattcttt gaagaacatg tctacggtta aaagtgttac
     6661 cttttcaagt aaagaagaac aatatgaaaa attaaccgag ataatgggag ataactggaa
     6721 aatctttgaa ggagatgcca atcctctcta tgatgcctat attgtagaga caaacactcc
     6781 aaatgatgta aaaactatag ccgaagatgc taaaaaaatt gaaggtgtct ctgaggttca
     6841 agatggcggt gccaatacag aaagactctt caagttagct tcatttatcc gtgtttgggg
     6901 actagggatt gctgctttgt taatttttat cgcagttttc ttgatttcaa ataccattcg
     6961 tattaccatt atttcccgca gtcgcgaaat tcaaatcatg cgcttggtcg gagctaaaaa
     7021 cagttatatc cgtggaccgt tcttgttaga aggagccttt atcggtttat tgggagctat
     7081 cgcaccatct gttttggtct ttattgttta tcaaattgtt taccaatctg tcaacaaatc
     7141 gttggtaggg caaaatctat ccatgattag tccagattta tttagtccgt tgatgattgc
     7201 cctactattt gtgattgggg ttttcattgg ttcattggga tcaggaatat ccatgcgccg
     7261 attcttgaag gtttaggtaa aatagctgct tttatgagga gattgtaaaa tctccttttt
     7321 tgctacaaga gtttttgaaa agagatgcgc agaagaaaag agcttccaaa gaagtccccc
     7381 agagaagact tcttgatggc taatttgaac ttgaaatttg gcgcactaga aattttttag
     7441 aaaaaagtgt aattttttta taaaagcctt tacaaaaaaa attaaagtgg tataattaaa
     7501 tcatagaaag tgcaaacgtt ttcgtaaaac gtttgcctaa ataaaaataa aggagatttg
     7561 aatgatgaaa gatacattca aaaatgtctt gtctttcgaa ttttggcaaa aattcggtaa
     7621 ggctttgatg gtagttatcg cggttatgcc ggctgctggt ttgatgattt caatcggtaa
     7681 gtctatcgtg atgattaacc caacctttgc accacttgtc atcacaggtg gaattcttga
     7741 gcaaatcggt tggggggtta tcggtaacct tcacattttg tttgccctag ccattggagg
     7801 aagctgggct aaagaacgtg ctggtggtgc tttcgccgct ggtcttgctt tcatcttgat
     7861 taaccgtatc actggtacaa tctttggtgt atcaggcgat atgttgaaaa atccagatgc
     7921 tatggtaact actttctttg gtggttcaat caaagttgct gattacttta tcagtgttct
     7981 tgaagctcca gccttgaaca tgggggtatt cgtagggatt atctcaggtt ttgtaggggc
     8041 aactgcttac aacaaatact acaacttccg taaacttcct gatgcacttt cattcttcaa
     8101 cgggaaacgt ttcgtaccat ttgtagttat tcttcgttca gcaatcgctg caattctact
     8161 tgctgctttc tggccagtag ttcaaacagg tatcaataac ttcggtatct ggattgccaa
     8221 ctcacaagaa actgctccaa ttcttgcacc attcttgtat ggtactttgg aacgtttgct
     8281 cttgccattt ggtcttcacc acatgttgac tatcccaatg aactacacag ctcttggtgg
     8341 tacttatgac attttaactg gtgcagctaa aggtactcaa gtattcggtc aagacccact
     8401 atggcttgca tgggtaacag accttgtaaa ccttaaaggt actgatgcta gtcaatatca
     8461 acacttgtta gatacagtac atccagctcg tttcaaagtt ggacaaatga tcggttcatt
     8521 cggtatcttg atgggtgtga ttgttgctat ctaccgtaat gttgatgctg acaagaaaca
     8581 taaatacaaa ggtatgatga ttgcaacagc tcttgcaaca ttcttgacag gggttactga
     8641 accaatcgaa tacatgttca tgttcatcgc aacacctatg tatcttgttt actcacttgt
     8701 tcaaggtgct gccttcgcta tggctgacgt cgtaaaccta cgtatgcact cattcggttc
     8761 aatcgagttc ttgactcgta cacctattgc aatcagtgct ggtattggta tggatatcgt
     8821 taacttcgtt tgggtaactg ttctctttgc tgtaatcatg tactttatcg caaacttcat
     8881 gattcaaaaa ttcaactacg caactccagg gcgcaacgga aactacgaaa ctgctgaagg
     8941 ttcagaagaa accagcagcg aagtgaaagt tgcagcaggc tctcaagctg taaacattat
     9001 caaccttctt ggtggacgtg taaacatcgt tgatgttgat gcatgtatga ctcgtcttcg
     9061 tgtaactgtt aaagatgcag ataaagtagg aaatgcagag caatggaaag cagaaggagc
     9121 tatgggtctt gtgatgaaag gacaaggggt tcaagctatc tacggtccaa aagctgacat
     9181 tttgaaatct gatatccaag atatccttga ttcaggtgaa atcattcctg aaactcttcc
     9241 aagccaaatg actgaagcac aacaaaacac tgttcacttc aaagatctta ctgaggaagt
     9301 ttactcagta gcagacggtc aagttgttgc tttggaacaa gtaaaggatc cagtatttgc
     9361 tcaaaaaatg atgggtgatg gatttgcagt agaacctgca aatggaaaca ttgtatctcc
     9421 agtttcaggt actgtgtcaa gcatcttccc aacaaaacat gcttttggta ttgtgacgga
     9481 agcaggtctt gaagtattgg ttcacattgg tttggacaca gtaagtcttg aaggtaaacc
     9541 atttacagtt catgttgctg aaggacaaaa agttgcagca ggagatctcc ttgtcacagc
     9601 tgacttggat gctatccgtg cagcaggacg tgaaacttca acagtagttg tcttcacaaa
     9661 tggtgatgca attaaatcag ttaaattaga aaaaacaggt tctcttgcag ctaaaacagc
     9721 agttgctaaa gtagaattgt aatatacttg aggttggaag ctgtattcca acctcttatt
     9781 ttgggagaaa agaatgaaat ttttaacact caatactcac agttggatgg agaaagaagc
     9841 agaggaaaaa ttccagattt tgcttgaaga tattcttgaa aaggactatg atttgatttg
     9901 tttccaagaa atcaatcagg agatgacctc gtcagaggtg gaggttaatg acctttatca
     9961 agctttgcca gcagctgagc ctattcacca agaccattat gttagactct tggttgaaaa
    10021 gttgttagag caagggaaaa attactactg gacctgggct tataaccata tcggctatga
    10081 ccgctaccac gaaggtgtgg ctatcttgtc taaaacacct attgaagcca gagaaatttt
    10141 ggtttcagat gtggatgatc caacagacta tcatactcgc cgtgttgccc tagctgaaac
    10201 tgtagtcgat ggcaaggagc tagcagttgc cagtgttcat ctctcttggt gggataaagg
    10261 tttccaagaa gaatgggcac gatttgaggc tgtcttgaaa aaattgaaca agccactttt
    10321 actagctgga gattttaaca acccagcagg tcaggaaggt taccaagcta ttttagctag
    10381 tccattaggc ttacaagacg catttgaagt tgctcaagag aaaagtggta gctatactgt
    10441 tccgcctgaa attgatggct ggaaagggaa cactgaaccc cttcgaatcg attatgtctt
    10501 tactaccaaa gagttagcgg tggaaaattt acatgtcgta tttgatggta acaagagtcc
    10561 acaagtgagt gatcactatg gcttgaatgc tatattaaac tggaaataat aactgaaaag
    10621 aggttggaac tataaaattc cagccttttc ttactagaga agctactgga aatagcctaa
    10681 ataagtgaga ctactgtaat ggaataaaat atggtataat tgataagata gatagaatcg
    10741 aggatgttat gtcatttacg aaatttcaat ttaaaaacta tattagagaa gccttgaagg
    10801 agttaaaatt tacaactcca acagaggtgc aagacaagtt gattcctatt gttttggcag
    10861 gtcgtgacct agtaggagaa tcaaaaacag gttcaggtaa gactcatact ttcttgttac
    10921 cgattttcca gcaattagat gaagctagcg atagtgtaca agcagtgatt actgcaccga
    10981 gtcgtgagtt ggctactcaa atttaccaag tagcgcgtca gatttcagct cactcagatg
    11041 tcgaagttcg tgtggttaat tatgtgggtg gtacggataa ggctcgccag attgagaaat
    11101 tggaaagcaa tcagcctcat attgttattg gaacaccagg ccgtatctac gacttggtta
    11161 aatctggtga tttagctatt cataaagcca agacatttgt tgttgatgaa gcagatatga
    11221 ccttggatat gggattcttg gaaactgttg ataagattgc tggcagtctt ccaaaagact
    11281 tgcaattcat ggtcttctca gcgactatcc cacaaaaact gcaaccattc ttgaaaaaat
    11341 acttatcaaa tcctgttatg gagaaaatta agaccaaaac ggttatttct gacaccattg
    11401 ataattggtt gatttcgacc aagggacatg ataagaatgc tcaaatttac cagttgactc
    11461 agttgatgca gccgtatttg gcaatgattt ttgttaacac taaaacgcgt gctgatgaat
    11521 tgcattcata tctgactgct caaggcttga aggttgcaaa aatccatgga gatattgccc
    11581 ctcgtgaacg caagcgaatc atgaatcagg tgcaaaatct ggattttgag tatattgtcg
    11641 caacagattt agcagcgcgt gggattgaca ttgaaggtgt cagccatgtc atcaatgatg
    11701 ccattccgca agacttatct ttttttgttc atcgtgttgg tcgtactgga cgaaatggcc
    11761 taccaggtac agctattacc ctttatcagc caagtgatga ctcggatatc cgtgagttgg
    11821 agaaattggg aatcaagttt agtcctaaga tggtcaaaga cggggaattt caagatacct
    11881 atgaccgtga tcgtcgtgcc aaccgtgaga aaaaacaaga taaacttgat atcgaaatga
    11941 ttggtttggt taaaaagaaa aagaaaaaag tcaaaccggg ttataagaag aaaattcaat
    12001 gggcggttga tgaaaagcgc cgtaaaacca agcgtgctga aaatcgcgct cgcggtcgtg
    12061 cagagcgtaa agctaaacgc caaacatttt aatagaaatt gttggagtat tgagctccaa
    12121 cttttttatt tatgagaacg aactatctaa accgaaacac tacattaaag actgcaaatt
    12181 gcgattaaaa atggtataat gataaagtta tatagtcccg ataagatggt aggtatttat
    12241 tacgaagagt tttcctatca gtactttgta actctataac aatatttttt aaggggggac
    12301 atttttatgt cagaacgtaa attattcacg tctgaatctg tatctgaggg gcatccggat
    12361 aagattgcag accaaatttc agatgcgatt ttggatgcta ttttagcaaa ggatccagag
    12421 gcgcacgttg ctgctgaaac agctgtatat actggttctg tccacgtttt tggtgaaatt
    12481 tctacaaatg cctatgtgga tattaaccgt gtggttcgtg ataccattgc agagattggt
    12541 tataccaata cagaatatgg attttctgct gagacggtgg gagtacaccc atctttggtg
    12601 gaacaatctc ctgacatcgc tcaaggtgtt aacgaagcct tggaggttcg tggaaatgct
    12661 gatcaagatc cactggactt gattggagca ggtgaccaag ggctcatgtt tggatttgca
    12721 gtagatgaaa cagaagagct catgccattg ccaattgcac tcagtcataa attggttcgt
    12781 cgtctggcag aacttcgtaa gtctggagaa attagctatc tccgtccaga tgcaaaatca
    12841 caagttacag ttgagtacga tgaaaatgac cgtccggtac gtgtagatac agtcgttatt
    12901 tctactcagc atgatccaga ggccactaat gaacaaatcc atcaagatgt gattaacaag
    12961 gtcatcaaag aagttattcc atcttcttat cttgatgata agacaaaatt ctttatcaat
    13021 ccgacaggtc gttttgtaat cggtggtcct caaggggact caggtttgac tggtcgtaag
    13081 attattgtag atacttatgg tggctactct cgtcatggtg gtggtgcctt ctctggtaaa
    13141 gatgcgacta aggtggatcg ttcagcctct tatgcggctc gctatattgc caagaatatc
    13201 gttgcagcag gccttgctaa gaaggcagaa gtgcagttgg cctatgctat cggtgttgcg
    13261 caacctgttt ctgttcgtat cgatactttc ggtacaggaa cagtagctga aagtcaactt
    13321 gaaaaagcgg ctcgtcaaat ctttgacctt cgccctgcag ggattatcca aatgctggac
    13381 ctcaagcgtc caatttaccg tcaaacatcg gcttacggtc acatgggacg tacagatatt
    13441 gatcttccat gggaacgttt ggataaggta gatgctttga aagaagcagt aaaataagat
    13501 tttaagaggg gaacgtcctc tcttttttat agtttttaac tatactggga tactgttctg
    13561 aaaatccatt ttgcgaaagt agagatttac atgtatagta gattgaaact agaatagtac
    13621 acctcaactt ctaaaacatt gttagcaatc aatttgactg tcctgatcga tttctcctgt
    13681 tcttgtttca ttttactata tttctttaaa aatgataaag gttgagattt ctcctcgtaa
    13741 tagataatca tggggatatt tcaatccaaa gttttattcg ttatcacttg actattgcaa
    13801 ggttttctag agcaacagag tcatggaatg gactcatggt tgagatttct ccttgttgct
    13861 tggacttcat tcaaaagtct gttacccaag ccttgttcaa acttccaata cactagctgt
    13921 tttcatagca tgacttctgt actagacttt cttttccgaa taaatagata gaaccacaga
    13981 atctagtaaa cctagaatta aaattatggt ataatattag caataaaaga aatctggagg
    14041 attagaatca tggtatcaac gaaaacacaa attgctggtt ttgagtttga caattgcttg
    14101 atgaatgcag caggtgtggc ttgtatgacg atagaggagt tagaagaggt caaaaactca
    14161 gcggcaggaa cctttgttac taagacagcg accttggact tccgtcaggg gaatcctgag
    14221 ccacgctacc aagatgttcc acttggttcc atcaactcta tgggcttgcc aaataatggc
    14281 ttagactatt atttggatta tcttttagat ttgcaggaaa aagagtcgaa ccgaactttc
    14341 ttcttatctc tggtcggcat gtctccagag gaaacccata ctattttgaa aaaagttcaa
    14401 gagagtgatt ttcgtggtct gactgagcta aatctttcct gtccaaatgt tccaggtaaa
    14461 cctcagattg cctatgattt tgagacaaca gaccggattt tggcagaagt gtttacttac
    14521 ttcaccaaac ctcttggaat taaattgcca ccttattttg atattgttca ctttgatcaa
    14581 gcggcagcta ttttcaacaa atatccgctc aagtttgtca actgcgttaa ctctatcgga
    14641 aacggcctct atatagaaga cgaatctgtc gttattcggc ctaagaatgg ttttggtgga
    14701 attggtggag aatacatcaa accgactgct ctagccaatg ttcacgcctt ttatcaacgt
    14761 ttaaatcctc aaatccaaat tatcggaaca ggtggcgttc tgactggtcg agatgccttt
    14821 gaacacatcc tctgtggagc aagtatggtg caggtgggaa cgacccttca caaagaaggc
    14881 gtcagtgctt ttgaccgcat taccaatgaa ctgaaagcaa tcatggtgga aaaaggctac
    14941 gagagcttag aagatttccg tgggaaattg cgctatattg actaaattaa atcgaaaaat
    15001 ctgaagaaag gagagacgat gctagccatt gaagaaagtc agaagttgac tttatcaaat
    15061 ttaccgagcc tgagcctatt tacagggaca gatcagggtc agtttgaagt gatgaagagt
    15121 caagtgttga aacagattgg gtatgattct gctgacctca actttgccta ctttgatatg
    15181 aaagaagtag tttacaagga tgtggaactg gagttggtca gccttccttt ctttgcggat
    15241 gagaaaatcg tgatattaga ttattttatg gatatcacga ctgctaagaa acgctttttg
    15301 acagatgatg agcttaagtc atttgaggaa taccttgaca atccttctcc aacaaccaag
    15361 ttgataatct ttgcagaagg aaagctggat agcaaaagac ggttagtcaa attacttaag
    15421 cgtgatgcca aggccttcga tgcagtagaa gtaaaagaac aagaattgcg ccagtatttc
    15481 caaaagtgga gtcagaaaca aggtctgcag tttaccaatc attcttttga aaatctcctc
    15541 atcaagtcgg ggtttcaatt tagcgaaatc cagaaaaatc ttctcttttt acagtcctat
    15601 aaggcggatt ctgttatcga ggaagaggat attgttaacg caattcccaa gaccttgcag
    15661 gacaatattt ttgatttaac tcagtttatt ctgactaaaa agatggatca ggcgcgcgat
    15721 ttggtgagag acttgacctt gcaaggggaa gatgaaatca aactaattgc agtcatgctg
    15781 ggacaatttc ggacttttac tcaggtgaag attttggcgg agtctggcca aacagaatcg
    15841 cagattgcaa gtagtttagg tagttatctg ggacgtaacc caaatcctta tcaaatcaag
    15901 tttgcattaa gagattcgag aggactttct ttgagctttc tgaagcaagc tatttcctat
    15961 ttgattgaga cagactatca gattaagaca ggtctttatg aaaaaggttt cctttttgaa
    16021 aaggcactct tacagattgc tagtcaggtc aattgacatt tgttgaaact acaacccgcg
    16081 tcaggattat cttgtcgtgg gtttcttgct gatttgctta tctataagca aaaaacttga
    16141 aaaaagtgaa tgtttgcgtt atcattttca tatagaaaga aaaagaggta ttacagatgg
    16201 ctattatctt accagaactt ccatatgcat acgacgcttt ggaaccatac atcgatgcgg
    16261 aaacaatgca tttgcaccat gacaaacacc atcaaactta tgtcaacaat gccaatgcag
    16321 ctttagaaaa acaccctgaa atcggtgaag accttgaagc cttgcttgct gatgtagaat
    16381 ctatcccagc tgatatccgt caagcactta tcaacaatgg tggcggacac ttgaaccacg
    16441 ctcttttctg ggaattgatg actcccgaga aaacagctcc ttcagcagaa ctggcagcag
    16501 caatcgatgc aacatttggt tcatttgaag aattccaagc agccttcact gcagcagcaa
    16561 caactcgttt tggttcaggt tgggcatggt tggttgtcaa caaagaaggg aaacttgaag
    16621 tgacttcaac agcaaaccaa gacacaccaa tctcagaagg taaaaaacca atcttgggct
    16681 tggacgtttg ggaacatgct tactacgtga aataccgcaa cgtgcgtcct gactacatca
    16741 aagctttctt ttcagtaatc aactggaata aagtagatga attgtacgca gctgctaaat
    16801 aatgatagtt ggagggaaga attgttcttc tcttttttag gttataagat tctggtctga
    16861 caaaatcgtt agactttttt cattttttat gagaaacgtg ctaactgcta gaaattatgg
    16921 tagaataaag tattaagtaa tcgtggaaaa aggatatcta tgcgaaaaga aattgcacct
    16981 gaattataca actataacaa gtttcctggt ccggagttcc atttacacgg ggacaaggtc
    17041 gaaacggaag ggatagcttt ttccttggtt gaaaatatca aggatgcctt tgatgtgacg
    17101 acttttaatc agcgtttttc agaagtctta accaagtttg attatatcgt gggggactgg
    17161 agcaacgaac agcttcgcct acgaggtttt tacaaggatg accgaacaga agaaaaactt
    17221 gaaaaaatca gtcgtttaca agactacctt ttagagtatt gtagttatgg ttgtgcctat
    17281 tttgtcttag aaaatgaagc ccctaagcga gcatcatttg acaagaaaat gcgtaagacg
    17341 gaagaagaaa caccttctag aaaaggaaag aaaccgactc aaactaaacg aaagtcgaat
    17401 gcagataaga aaaatagacg tcgtcagaaa gaccagcatt ctcagaaaga ggacaaggaa
    17461 caacgtcatt ttgtcattcg tcagaagtga gtagagaata aggagaagaa atgaaaccct
    17521 caattcatag tttggtacat caaaccatgc aggaatgggt attggagcag ggagaaaaga
    17581 aattccgtgc agatcaaatc tgggaatggc tctaccgtaa acgtgtgcag tcatttgaag
    17641 aaatgaccaa cctttccaag gatttgattg ctaagctcaa tgaccagttt gtggtcaatc
    17701 ccttgaaaca acgtattgtg caagagtctg ctgatggtac agtcaaatat ctctttgaat
    17761 tgcctgacgg tatgttaatt gagactgtac tcatgcgtca acactatggt ttatctgtct
    17821 gtgtgaccac tcaggtcggc tgtaatatcg gttgtacctt ctgtgcctct ggtttgatta
    17881 agaagcagcg tgacctcaat aacggtgaaa tcgtagcgca aatcatgctg gttcagaaat
    17941 actttgctga tgaaggggtt caggtcaatc ttgccgtgtc tcttcacgca cccaacaatg
    18001 aacttcgttc aagtatcatg aagattaacc gtgcctttcc gattgaaaaa ctctttgctg
    18061 ctattgaata ctacattgag acaacaaacc gtcgtgtaac ctttgaatat atcatgctta
    18121 atgaagtcaa cgacggtgta gaacaagcct tggaattggc tgaattgctc aagaacatca
    18181 agaaattgtc ttatgtaaac ttgattcctt ataacccagt tagtgagcat gaccaatata
    18241 gccgtagtcc caaagagcgc gtgttggctt tctatgatac gcttaagaaa aaaggggtca
    18301 actgtgtggt tcgtcaagag catggtacag atattgacgc agcttgtgga caattgcgct
    18361 ctaacacaat gaaacgtgac cgccagaagg cagtcgcagc ggtaaatcct taatgatgaa
    18421 gaagacttat aatcatattt tggtctgggg agtcattttc tatagcattt gcattgtctg
    18481 tttttgcttt actcctcaag aacaatctac cgtgggagtg ggaactccag gtattcagca
    18541 tcttggacgc ctggtttttc ttttgactcc tttcaattct ctctggaaac tgggcgaagt
    18601 gagtgacatt ggacaattat gttggatttt tttacaaaat gtcctcaatg tcttcttgtt
    18661 ttttcctctg attttccaac tcctttatct atttccaaat ttgcggaaaa caaaaaaggt
    18721 ccttcttttt agttttcttg tgagtcttgg aatcgagtgt acgcaattaa tcttggactt
    18781 tttctttgat ttcaatcgcg tctttgagat tgatgatttg tggaccaata ctttgggtgg
    18841 ctatctggct tggctccttt ataaacgatt acataaaaac aagataagga attaaaatga
    18901 gtattttaga agttaaaaat ctgagtcacg gttttggtga ccgtgcaatt tttgaagatg
    18961 tgtccttccg tctcctcaag ggagaacata tcggcctggt cggtgccaat ggtgaaggaa
    19021 aatcaacctt tatgagtatc gtgactggta aaatgctgcc agatgaagga aaggttgagt
    19081 ggtccaaata tgtgacggct ggttacttgg atcagcactc tgtccttgct gaaagacagt
    19141 cggtgcgtga tgttctccgt acggcttttg atgagctttt caaagctgaa gctcgtatca
    19201 atgacctcta tatgaaaatg gctgaagacg gcgcggatgt tgatgctctc atggaagaag
    19261 taggagaact tcaagaccgt ctggagagtc gtgatttcta taccttggat gctaagattg
    19321 acgaagtagc gcgtgctctt ggtgttatgg actttggcat ggatacggat gtaacttctt
    19381 tgtcaggtgg gcaaagaacc aaggtgcttt tggcaaaact tctccttgaa aagcctgata
    19441 tcttgctgtt ggacgagccg accaactact tggatgctga gcatattgat tggctcaagc
    19501 gctatctcca aaactatgag aatgcctttg ttctcatttc gcacgatatt ccattcctca
    19561 atgacgttat taatattgtc tatcatgtgg aaaatcaaca gctgacgcgt tactctggtg
    19621 actactacca gttccaagaa gtttatgcta tgaagaaatc tcagctagag gcagcctacg
    19681 aacgccagca gaaagagatt gcagacctca aggactttgt ggctcgtaat aaagcccgtg
    19741 ttgcaactcg taatatggct atgtctcgtc aaaagaaatt ggataagatg gatattatcg
    19801 aactccaaag tgagaaacca aaaccatcct ttgatttcaa accagctcgt acaccagggc
    19861 gctttatctt ccaagccaag aacttgcaaa ttggttacga ccgtcctctt actaagcctt
    19921 taaatcttac cttcgaacgc aatcaaaagg ttgcgattat tggtgctaat ggtattggaa
    19981 aaacaactct cttgaagagt ctcttgggca ttatctcgcc aatcgctggg gaagtggagc
    20041 gtggagatta tttagaactt ggttattttg agcaggaagt agaaggtggt aatcgccaaa
    20101 ctcctcttga agctgtctgg aatgcctttc ctgcccttaa tcaagcagaa gtccgtgcag
    20161 cccttgcccg ttgtggtttg acaaccaaac atattgaaag ccagattcaa gtattatcag
    20221 ggggagagca agccaaggtt cgtttctgtc tcttgatgaa tcgtgaaaac aacgttttag
    20281 tgctggacga gccgaccaac catttggatg tggatgcaaa ggatgagctc aaacgcgctc
    20341 tcaaagaata caggggatct atccttatgg tctgccacga gccagacttt tatgaaggtt
    20401 ggatagacca aatatgggat tttaataatt taacttaaga atgagagtaa aagaaatacg
    20461 gctctttgtc aactgtagtg ggttgaagtc agctaagctc gagaaaggac aaaattcgtc
    20521 ctttcttttt tgat
//
LOCUS       DAVMGZ010000024        19542 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000024 DAVMGZ010000000
VERSION     DAVMGZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.025"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(144..758)
                     /locus_tag="VYQ93_000729"
     CDS             complement(144..758)
                     /locus_tag="VYQ93_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV3335460.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1573..1842)
                     /gene="rpsO"
                     /locus_tag="VYQ93_000730"
     CDS             complement(1573..1842)
                     /gene="rpsO"
                     /locus_tag="VYQ93_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV3335461.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(1983..2612)
                     /locus_tag="VYQ93_000731"
     CDS             complement(1983..2612)
                     /locus_tag="VYQ93_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV3335462.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            2849..3049
                     /locus_tag="VYQ93_000732"
     CDS             2849..3049
                     /locus_tag="VYQ93_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335463.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            3480..3803
                     /locus_tag="VYQ93_000733"
     CDS             3480..3803
                     /locus_tag="VYQ93_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV3335464.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDPLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            3861..5804
                     /gene="thrS"
                     /locus_tag="VYQ93_000734"
     CDS             3861..5804
                     /gene="thrS"
                     /locus_tag="VYQ93_000734"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV3335465.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFEKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5853..6827)
                     /locus_tag="VYQ93_000735"
     CDS             complement(5853..6827)
                     /locus_tag="VYQ93_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV3335466.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6820..7497)
                     /locus_tag="VYQ93_000736"
     CDS             complement(6820..7497)
                     /locus_tag="VYQ93_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3335467.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7653..8726
                     /locus_tag="VYQ93_000737"
     CDS             7653..8726
                     /locus_tag="VYQ93_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV3335468.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEVFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8817..9119)
                     /locus_tag="VYQ93_000738"
     CDS             complement(8817..9119)
                     /locus_tag="VYQ93_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335469.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9187..9624)
                     /locus_tag="VYQ93_000739"
     CDS             complement(9187..9624)
                     /locus_tag="VYQ93_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV3335470.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9700..10542)
                     /locus_tag="VYQ93_000740"
     CDS             complement(9700..10542)
                     /locus_tag="VYQ93_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV3335471.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10654..11304
                     /locus_tag="VYQ93_000741"
     CDS             10654..11304
                     /locus_tag="VYQ93_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV3335472.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11481..12127
                     /locus_tag="VYQ93_000742"
                     /pseudo
     CDS             11481..12127
                     /locus_tag="VYQ93_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            12258..12672
                     /locus_tag="VYQ93_000743"
                     /pseudo
     CDS             12258..12672
                     /locus_tag="VYQ93_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12691..13105
                     /locus_tag="VYQ93_000744"
                     /pseudo
     CDS             12691..13105
                     /locus_tag="VYQ93_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13144..13587)
                     /locus_tag="VYQ93_000745"
     CDS             complement(13144..13587)
                     /locus_tag="VYQ93_000745"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV3335473.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(13617..15839)
                     /locus_tag="VYQ93_000746"
     CDS             complement(13617..15839)
                     /locus_tag="VYQ93_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV3335474.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            16835..17464
                     /locus_tag="VYQ93_000747"
     CDS             16835..17464
                     /locus_tag="VYQ93_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV3335475.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(17587..19479)
                     /gene="pepO"
                     /locus_tag="VYQ93_000748"
     CDS             complement(17587..19479)
                     /gene="pepO"
                     /locus_tag="VYQ93_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV3335476.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         5709 a         4439 c         3638 g         5756 t
ORIGIN      
        1 ttataagaat ctcctaatca aatcaaaagc cactctacaa tagagtaact ttaagattgt
       61 ccatttatac tcaatgaaaa agccccctga aaatcagaga gctgatttga gctcgggcta
      121 aaatcctagt gaaacaaaaa aatctacacg atcagaaaag tctctatcgt gccattttca
      181 tacatgatgt atagtccaag tagaatgaat actaagggca caatgattcg ctcgtatttt
      241 tcaattgtct cgaatattaa cggaatagag gataacaccc gactaatctc gcaaaagata
      301 attatgccga ttacaaacac aagcaaggcc acgagggtct gtgaccaatc taacgaagca
      361 aaataaggta tatagatacc taaattatct ccgccagacg caattgtcag caatgtaact
      421 gtccaaaaca gttgatttgc cttgctttgt tctaatcttt caataatttc ttcctcttct
      481 tcttccgcat cttctccaac aattgcaaag cgaatcccta aatagatagg gattaaacca
      541 agcaatccaa ccatccattc ttcaggcacg aaattaacga cataagcagc aactaaactc
      601 gcccctacaa gtaagcctgt gcctagatat tgccccgcat aaatatgcca tttctgttta
      661 ttctgtgata gctgtgcaaa taaaataatt aaaataatta aataatcgat actggtggaa
      721 atataaacac caatagcaga tatgattgtc tgtcccataa aaaaaacctc ctgtattagt
      781 cagtaataaa tcagcagatt ttaggagagc acagacacat taatttagct atcgctagtg
      841 ggtaatgtcg aacgtaggaa agcattttac tcctcctcaa aacgtagtta caaagtaatt
      901 tctaactgga attcagtgac tacttccatg taaagtataa catactatac tttactttgt
      961 aaagagtggg ctcttctagg gggcttgcat acagctactc ggcttgaaaa gccgagtaac
     1021 tgcgcacctt tatggtgtaa ttagctgata ccatttgagg tttttgtagt ctccaaaatt
     1081 gttaccgata aaacctcaat aaaaaagccc tctgaagtca gagagctgat tttagcccgg
     1141 gctaaaatcc tagtgaaaaa gatgaaactc cttgtgctca tcgaacactg tgtcatttcc
     1201 ctattttcat acggattttt gacgccctta gcatcatgat tcttgctaga taaatcgttt
     1261 atagactagg ggacgaggtg aagatcgtac gaaactttca gtgaaacaaa aaaatctccc
     1321 cgaagggaga aaagctagtt tattttacct tacagtgcta ggaaccgtaa ccgaagatta
     1381 tacttgagta tagcgaggta aggtgacaac gcaacaaagc tacccaattt agagtagctt
     1441 tattatcaag atatgctcaa ttgaacacgg cctaagcact tggcaaaaaa gataaaatct
     1501 cctagaaact gaagtttctt cgtcaatttt cctatttttg ctttgtgttt ttgacggcct
     1561 ttgtatcttg aattagcgac gaagtcctag agagttgatc aactcacggt aacggttaac
     1621 gtcgttttta cgcaagtatg caagcaagtt acgacggcga ccgatttttt tcatcaatcc
     1681 acggtaagta gcgtggtctt ttttgtgttg tttgatgtgt tcgttaaggt ggttgatttc
     1741 ccaagtaagg acagcaactt gaacctctac tgaacctgta tcaccttcgt gacgtgcata
     1801 ttgtgcaatg atttcatttt ttttctcttt tgagattgcc atgatgtttc tcctttttat
     1861 ttggcttcat ccgagtgaca ggttggcatg cctgtaacca agaagaagtt atttgtcttt
     1921 acgacaatcc ctattttacc aagatttaaa ttttctgtca aacattaata ctctcatagt
     1981 tattattttt caagtaaaga gatacgttct tttatatgat gacctttttc aagttcatct
     2041 accaatccaa tagcataatc tgcataacta atctggctta ttcctttttc attagtagta
     2101 aaaatttctc ctgctagaat gtagtttcct gttttttcac catcgggaat aaaatctaca
     2161 gcaggactga caaaagtcca gttgagatta ttttttgtac gaagaagatc caattcatcc
     2221 gcttgagctt ttgctagtga cttaaattct tcaggaaaat cagacgtatc taaaagtcgt
     2281 gtcgtttttg tttcatctat ataaagactt cctgctcccc ctacaactag aaaacgtgtc
     2341 tgtgttccag caagaagctg attaaatagt tcgattgatt tgctgtggag cggtagcgta
     2401 tctggtgtat aagcaccaaa cgctgaaata acagcatcaa atccagtaag atcatctttt
     2461 gtcaactcaa ataaatcttt tttaataata gactcagatt gacttttgtt ttcagaacga
     2521 acaatagccg ttacttcatg tcctcgtttg actacttctt caacaattgc tttccccgct
     2581 tgtccatttg ctgcaataac tgctagtttc attttttata cctctcttat tgtaattatt
     2641 ttagttacaa caattgtgac actcttaata atcaatgtca atagttttgc ttaattatta
     2701 tcaaaatatt tctaccaaga aaactaacca tgattctagt gaaaaaaaat cttctttgcc
     2761 aacaaattta ctttcttgtt ttaaacatgc tataataatc atagcaagag atctaagttg
     2821 tctgtttttt taaaacgagg tgattatcat gcgtagattc tattcccatc tcccctacta
     2881 tctggtcata ttattctttt attggccact ttatgagttg ttcttactag ttgtttctga
     2941 cccccttaca ctcaaggaac tctatataaa caatcttctc ttctttacac ctctggtaat
     3001 cttgattgta tcgttactct atagctaccg tttccgtttc tcactttgat ggttagttgg
     3061 taacggactg ctcttttact ttactatcat aacctttggt gagtttatac taatttactt
     3121 gctaatctat gaaacagttg ctctggtggg catggattct ggtattagca tcaagcatat
     3181 tctacaaaaa atgaaaaaca aaaaactttc acaaaatcct tgaaaaatct cacaatcatg
     3241 ctataataat ccatagagac aagtcactta gtccctttct actagagagt gcgtggttgc
     3301 tggaaacgca taggaagtct aaactgatac tactcttgag ttttttatga aaacataaaa
     3361 cggtggccac gttagagcca atcagaggtg tccctctctt ttgaggtaca taaatgaagg
     3421 tggaaccacg ttgcgacgtc ctttcgagga tgtcgcattt ttttattagg atactaatta
     3481 tggagttgca agaattagtg gagcgcagtt gggcaatccg acaagcttat cacgaactgg
     3541 aagttaagca tcatgattcc aagtggacgg tagaagaaga ccccttggct ttatctaatg
     3601 atattggaaa tttccaacga ctggtgatga caaagcaagg acgctactat gatgaaacac
     3661 cctacacact ggaacaaaaa ctttcagaaa atatctggtg gctattagaa ctttctcaac
     3721 gtttggatat agacattctg acggaaatgg aaaacttcct ctctgataaa gaaaagcaat
     3781 tgaacgttag gacttggaag tagtctgatg ataaaaaatc aatgcttaga aactatgaaa
     3841 taataaaaaa ggagaacatc atgattaaca ttactttccc agatggcgct gttcgtgaat
     3901 tcgaatctgg cgtaacaact tttgaaattg cccaatctat cagcaattcc ctagctaaaa
     3961 aagccttggc tggtaaattc aacggcaaac tcatcgacac tactcgcgct atcactgaag
     4021 atggaagcat cgaaattgtg acacctgatc acgaagatgc ccttccaatc ttgcgccact
     4081 cagcagctca cttgttcgcc caagcagctc gtcgtctttt cccagacatt cacttgggag
     4141 ttggtccagc catcgaagat ggtttctact acgatactga caacacagct ggtcaaatct
     4201 ctaacgaaga ccttcctcgt atcgaagaag aaatgcaaaa aatcgtcaaa gaaaacttcc
     4261 catctattcg tgaagaagtg actaaagacg aggcacgtga aatcttcaaa aatgaccctt
     4321 acaagttgga attgattgaa gaacactcag aagacgaagg cggtttgact atctatcgtc
     4381 agggtgaata tgtagacctc tgccgtggac ctcacgttcc atcaacaggt cgtatccaaa
     4441 tcttccacct tctccatgta gctggtgcgt actggcgtgg aaacagcgac aacgctatga
     4501 tgcaacgtat ctacggtaca gcttggtttg aaaagaaaga cttgaaaaac taccttcaaa
     4561 tgcgtgaaga agctaaggaa cgtgaccacc gtaaacttgg taaagagctt gacctcttta
     4621 tgatttcaca agaagtggga caaggtttgc cattctggtt gccaaatggt gcgactatcc
     4681 gtcgtgaatt ggaacgctac atcgtaaaca aagagttggc ttctggctac caacacgtct
     4741 acactccacc acttgcttct gttgagcttt acaagacttc tggccactgg gatcattacc
     4801 aagaagacat gttcccaacc atggacatgg gtgacgggga agaatttgtc cttcgtccaa
     4861 tgaactgtcc gcaccacatc caagttttca aacaccatgt tcactcttac cgtgaattgc
     4921 caatccgtat cgctgaaatc ggtatgatgc accgttacga aaaatctggt gccctcactg
     4981 gccttcaacg tgtacgtgaa atgtcactca acgacggtca cctattcgtt actccagaac
     5041 aaatccaaga agaattccaa cgtgcccttc agttgattat cgatgtttat gaagacttca
     5101 acttgactga ctaccgcttc cgcctctctc ttcgtgaccc tcaagatact cataagtact
     5161 ttgataacga tgagatgtgg gaaaatgccc aaaccatgct tcgtgcagct cttgatgaaa
     5221 tgggcgtgga ctactttgaa gccgaaggtg aagcggcctt ctacggacca aaattggata
     5281 tccagattaa aactgccctt ggaaaagaag aaaccctttc tactatccaa cttgatttct
     5341 tgttgccaga acgcttcgac ctcaaataca tcggagctga tggcgaagat caccgtccag
     5401 tcatgatcca ccgtggggtt atctcaacta tggaacgctt cacagctatc ttgattgaga
     5461 actacaaggg tgccttccca acatggctgg caccacacca agtaaccctt atcccagtat
     5521 ctaacgaaaa acacgtggac tacgcttggg aagtagccaa gaaactccgt gaccgcggtg
     5581 tccgtgcaga cgtagatgag cgcaatgaaa aaatgcagtt caagatccgt gcttcacaaa
     5641 ccagcaagat tccttaccaa ttaattgttg gagacaaaga aatggaagac gaaacagtca
     5701 acgttcgtcg ctacggccaa aaagaaacac aaactgtctc agttgataat tttgttcaag
     5761 ctatcctagc tgatatcgcc aacaaatcac gcgttgagaa ataagagtct agcataaaag
     5821 cctccaatct ggaggctttt tctcatctat ttttactcaa ggactaagtt cacttgagca
     5881 aactgaatcc gcactgtcgt tccttttccg acctcagact cgatacgaat ctggtgcccc
     5941 agttcttcag aaattttctt agatagataa aggccaagtc cagaggactg ctgggtcaaa
     6001 cggccattgt atcctgaaaa gccacgttca aatactcgga ggacatcact gttttttatc
     6061 ccgattcccg tatctttgat acaaagctct tggtcatcca tataaatctc cagaccacct
     6121 tccttggtgt acttgagact gtttgagatg atttgctcaa taaccactag cagccacttt
     6181 ttatccgtca cgatttcttt atcaaggtca tgtagattga catttaagcc tttttgaata
     6241 aagaaaagag catatttacg aattatttcc ttgaccaagt cctcaatttg aacctgcttt
     6301 aagaccaaat catcatggaa actttctaaa cgcaggtact gtaaaactag gttggtatag
     6361 gagtcgattt tgaaaatttc ctgttctagc tgctgcttca gttggcggtc gaccacttct
     6421 gcaactaaga gttgactggc tgcaatgggg gtctttatct gatggaccca caaggtatag
     6481 taatccagca aatccgtcag ttttctttct gcttttgacc tctgctgata gagttccatc
     6541 tcacgcgctt ctaatttttc tgctaaagct atttccaaag gagacttggc ttccctctct
     6601 ccatagagaa gttcctggcg atagacctgc gtttccacca atatgtccca agtgaaaaat
     6661 aagatggtta caaagcaaca caagaagaaa aagtagagga agtaaattcc tagactggca
     6721 aataaaaact gaaagagtaa gacaagaaat gccaaagaaa gcagatagat aaaaagacga
     6781 ctacgggagc gcagataggc tagaaaaaat tgtttccaat caagcatgct tcaatccgta
     6841 ccctattcct ttcttggtct cgataaatcc taccaatccc tgctcctcca actttttacg
     6901 caaacgagcc acattgacag agagggtatt atcatcaatg aaaaagtcac tgttccaaag
     6961 ttcccgcatc aggtcgtcac gtgctacgat gttgcctgca tgctcaaata acacgcgtaa
     7021 aatctggaat tcattcttgg tcaaattcaa gacttgccct tgataatgta aatccatgga
     7081 tttggtattg aggataacac cagcatattc cagcaaactc tcatcacgcc caaactcata
     7141 ggaacgacgc aacaagccct gaaccttagc taaaagaacc tgctggtcaa aaggcttggt
     7201 cacaaagtca tccgccccca tattgattgc catgacaata tccatagcct ggtctctcga
     7261 agaaagaaac atgataggta ccttggaaat cttgcggatt tcctgacacc agtgataacc
     7321 attaaacaag ggcaaaccaa tatccatgag gaccagatga ggttccgact gaacaaatag
     7381 actcaaaact tccataaagt cttctaccag gaccacttca aatccccatt cagagagcat
     7441 tttcccgacc tgttgacgaa tgacctgatc atcttctatt aataaaatct tgtgcatgcg
     7501 cttctccttt tctattatta taacagattt ttccatgcca gatggtctga aactgaattt
     7561 gaaatagcct gtttttagcc agtacaaaca ggcaatgcta ctagctaatt tgagggaaat
     7621 ttgctaagat aaataaaaag aaaggagctc ttatggccaa tatttttgac tatctgaaag
     7681 atgtcgcata tgattcttat tacgaccttc ccttgaatga gttagacatt ctaaccttaa
     7741 tagaaatcac ctacctctcc tttgataatc tggtctccac acttcctcaa cgtcttttag
     7801 atctagcacc tcaggttcca agagatccca ccatgcttac tagcaaaaat cgccttcaat
     7861 tattagatga attggctcaa cacaagcgct tcaaaaattg caaactctcc cattttatca
     7921 acgacatcga ccctgaactg caaaagcaat ttgcggctat gacttatcgt gtcagcctcg
     7981 atacctatct gattgtcttt cgtgggacag atgacagtat cattggctgg aaggaagttt
     8041 tccacctgac ctatatgaag gaaattcctg ctcaaaagca cgcccttcgc tatttaaaga
     8101 acttttttgc ccatcatcct aagcaaaagg ttattctagc tgggcattcc aagggaggaa
     8161 atctcgctat ctatgctgct agccaaattg agcaaagttt gcaaaatcag atcacagcag
     8221 tttatacatt tgatgcacct ggtctccatc aagaattgac acagactgcg ggttatcaaa
     8281 ggataatgga tagaagcaag atattcattc cacaaggttc cattatcggt atgatgctgg
     8341 aaattcctgc tcaccaaatc atcgttcaga gtactgccct gggtggcatc gcccagcacg
     8401 atacctttag ttggcagatt gaggacaagc acttcgtcca actggataag accaacagtg
     8461 atagccagca agtagacaca acctttaaag aatgggtggc cacagtccct gacgaagaac
     8521 ttcagctcta cttcgacctc ttctttggca ctattcttga ggctggtatt agctctatca
     8581 atgacttggc ttccttaaag gcgcttgaat acattcatca tctctttgtc caagctcaat
     8641 ccctcactcc agaagaaaga gaaaccttgg gtcgccttac ccagttattg attgatactc
     8701 gttaccaggc atggaaaaat agataatact cttgaaaatt aaatgtatac aaaacaaaag
     8761 acctagaata catactttca tgtgcattct aagtcttttt aaatagaatc taatagtcaa
     8821 taaaaatcaa agagcattga gagataatgg ggcttggaac gtccctctcg cttcaacaaa
     8881 atgaccccat tatagattaa aaagatgcca cttagaaaaa gcaaaaaagg aagtaagaca
     8941 aaggcaaata cataaaaagc taactgaaca ttctcgtatc catttttata aaaaaggtag
     9001 gatagataaa aataacttga aatgagggat aataaaaata atactggatt ccacaaactt
     9061 ctattatcct tccaaaatga cactataaag gctaatacaa ttcctataac gagatacatt
     9121 tcttactcct ttaatagcta cattttatca taattatcca aagaaaaaag agggcattta
     9181 tccctcttaa tccttcatct gactctctgc atcggccacg actttttcta gactggtttg
     9241 accaagttct gcctccatag tcaactgaat tctctccaat ttttgatcca aaacatcatg
     9301 aatatgagct cctacagggc aatttggatt cggattgtca tggaaactga agagttgacc
     9361 tgtcttacca agacattcga ccgcctgata aacatctaaa agactaatat ccttaaggtc
     9421 cttgacaatc tctgttccgc ccgttccacg cgctactgaa atcagctctg ccttcttcaa
     9481 ctgggacaag atctttctga taatgacagg attgaccccg acactagcag ccagaaaatc
     9541 actggtcacc ttgctttcct tcccctcgag ggcaatgatt atcagcatat gagtcgcaat
     9601 ggtaaatcta cttggaattt gcatcctctt ctccttttta cgaggctacc ctgcctctac
     9661 tcttcttttt ctattattat acccttttta gttgtaatgt caatcgttac cacttttcaa
     9721 ccagtcgtct aactcccgat cgcagccctc tttctgagcc aattctctca aaaattcctg
     9781 atgatgagta tggtggatcc cattgaccag actttcatag taaacctcaa aatagggaag
     9841 tctcaggtct ttagccagct gcaattccgc tgccacatcg tagtctaccc gtcggaagtc
     9901 catatctacc aggcctttgt catcaaactc caaaatcata tactgggccc gcaagtcctt
     9961 ccgtagctga gcgtccaaaa agaaaggttg gccaatcgaa cccggattga caatcaattg
    10021 cccaccagtc ccgtaacgaa gcaactgctg gtgaatatgt ccataaacag caatatcaca
    10081 gggaggatga gtcaccaagc ggtcaaactc ctcttgtttg ccagtatgaa tcaactctcg
    10141 cccccagttc ttatcaggca gatgatggct aattcccacc gtcaaatccc caaactgacg
    10201 atgaatttga agaggttgat tgtggagcac ttcaatttct tctagggaaa tttcctctaa
    10261 aacatactgg cactggcgca agagatagcg ttgactgggg cgagtactgt ccaattcctt
    10321 acggacacca tgccaaagac tgtcttccca gtttcccaaa actctagccg taatcggtag
    10381 ttgatccaac aagtccaaaa tccttctacg tcctgtccct ggcatgagaa tatctcccaa
    10441 aagccagtat tcatccactc ctatctgctg agcatctgcc aaaacagcct ccaaggcggt
    10501 ggtatttcca tgaatatctg aaagaagagc tattttcgtc atatccatct cctcgttttt
    10561 tctcttgcaa taagtataac ataaaaagtc acagctagag aaatctagct ttttttgata
    10621 tactagataa agatattaga caagaggaaa cgaatgaccc caaacaaaga agactatcta
    10681 aaatgtattt atgaaattgg catagacctg cataagatta ccaacaagga aattgcggct
    10741 cgcatgcaag tctctccccc tgccgtaact gaaatgatca aacgaatgaa aagtgaaaat
    10801 ctcatcctaa aggacaagga atgtggctat ctactgactg acctcggtct caaactggtc
    10861 tctgagctct atcgtaagca ccgcttgatt gaagtttttc tagttcatca tttagactat
    10921 acaagtgacc agattcacga ggaagctgag gtcttggaac acactgtctc tgacctgttc
    10981 gtggaaagac tagataaact gctaggtttc cctaaaacct gcccccacgg aggaactatt
    11041 cctgccaagg gagaactcct cgttgaaatc aataacctcc cactagctga tatcaaggaa
    11101 gctggcgcct accgcctgac tcgggtgcac gatagttttg acattctcca ttatctggac
    11161 aagcactcac ttcacatcgg tgaccagctc caagtcaagc agtttgatgg cttcagcaat
    11221 accttcacta tcctcagtaa cgacgaggat ttacaagtga atatggacat tgcaaaacaa
    11281 ctctatgtcg agaaaatcaa ctaatttctc aagtccccta ccaaccctga aagttttatt
    11341 ttcagggttt tatttctatc atttgatttt actttcattt ggtggtagag tatttgaaaa
    11401 aaagaaaggt agtttaacct atgaaaaaat cactaaaaat ttttgctaca tctaaatgat
    11461 ttgacctctt cggggttgct ttggtcgttg ggattgcgat tgcatctggt tatctcaact
    11521 cacgcctcga taaatttgta gactggggac tatggacagc tcttgtaccc tttggattga
    11581 tttccgtaac caacgttggg atttccatgt tgtccactcg tttcacgggg aaattaagca
    11641 aatggggaaa ttactttggt attgttaata ccattttgtc cggtgctatt gattatatcc
    11701 ttggaaataa ggcggccatc attacctatc ccgtcacctt cctcatttat acctttgcta
    11761 taaagaaatg gaaagcttcg caagaaggca gacccaacca aatgagccaa aaacaggtaa
    11821 aattggcggc catcatcatt tccatcatcg ccttcctctt tgcctttgtg accaactata
    11881 tcggatatgg gggcaagatg aatctccttg cctacgtaac aactattgcc tttgcactgt
    11941 ccctcattgc caatgctttg aatgcattgg caatgaggga cagtggggct tttggttgat
    12001 ttacaatttc gttcagctga caaaagctgg tattcaagga aacttcgcca atatcggtaa
    12061 atacatcttt tatatcctca atgcaatcgg agctttattt gtctggaatg atgaagaagt
    12121 tagataatag aaggaaactc aaataaagaa tactagtcaa cttaagagaa tctcccctct
    12181 gctattttta tcattttagt tgtaactccc attcttttag agtagaatga agttagataa
    12241 aagagggagg tcttgtcatg aaaaaactct atagaatcca ttttatagca attgcagtta
    12301 ttgatttgct actatttgca ttttttatca ttagactcga gacatctttt gagtggctct
    12361 tgctctctgg tttaattttc ttccttgctc aaggactcct gctatttctc ttgatcgtcc
    12421 gactcaaaca tcaattcgct gagatttatc ctcaaatcaa taaaaagatt cgcttctact
    12481 atttaggggt tctcaccatt gattttctat ttttttgttc tcttagcctt cattagttct
    12541 cagcgttttt catctcttat gtcaattatc actgcttgcc attctacttt ttattatatg
    12601 acagctgact acctaagaga aaactatcca gacttttacg acaaacacat ctctttatgg
    12661 gagtgtctct aaggaaaagg aggttttatc atgaaaaaaa tcatcttcat caaaaccatt
    12721 caactccttg tcattgatgg aatcatgctg gcatttttga catttaaaag gggggcttac
    12781 ttgggactgg attttgattt atagcggttg gctcattttc tttcatcctg tgctattgac
    12841 ctatctttca aaccaacttt gtgaccactt tagtcaactc tattcccaga ttagaccgag
    12901 attctggcgt tttgctttac aaattctcct atgggatagc ctgatgattc tctccttggt
    12961 gtctttaagt gatattccac ttttccttca gggaactctc ctcatcctag gacatctcat
    13021 cccttcctat cgcatctgcc aaagcctgaa aagagacttc ccccaagcat atcaagaacc
    13081 gatttctttt tggagtattt tatgatagat gagaaagacc aagccgactg ggcttggtct
    13141 ttcttatctc tttttagtat ctaggataat ggtaacaggt ccgttattaa ccagctcaac
    13201 ctgcatatct gctccaaaga tacctgtctg aacgggcact tcttgcgcta atttttgatt
    13261 gaaagcatca tagaagtctg atgccatatc aggtttagct gcccctgtaa aggctggacg
    13321 attgcctctc ttagtatccg caaagagggt aaactgagaa atagagagga tttctccttc
    13381 aatatctttg acagacaggt tcatcttgcc ttctgcgtct gaaaaaatcc gcatattgac
    13441 cagttttctc acagcatagt ccaaatcttc ctcttggtcc tctggtccaa caccaactag
    13501 caataaaagt ccctgattga tttttccctg aatctggcct tctatactca cttgggcttt
    13561 tttaacccgt tggataatga ttttcataat agcctttcta gtaagagcta ggacaactag
    13621 ccgttggtcc gtttgacaga gtaaacttct ggcacactct taattttatc gacaaccgtg
    13681 gtcagtgtag agaggttggc aataccgaag gacacatgga tattagcaaa cttcatatcc
    13741 ttggttggtt gggcattgac cgttgaaata ttcttggttg tatttgaaag aacttgcagt
    13801 acatcgttca acagtcctgt acggttgaga ccgtagatat cgatatgggc catatactcc
    13861 ttatttgagc tagagtactg gtcttcccat tccacatcaa ggagacgttg ctcgtagttt
    13921 tcttgggcac gcaggttcat acagtccaca cggtgaatag ccacaccacg acccttggta
    13981 atgtagccaa caatatcgtc accaggcacg gggttacaac acttagcaat ccgcactagg
    14041 agaccagaag caccttcaat aaccactccc ccctcatgct tgaccttgag agtttcttta
    14101 ttttcaacct tgacctcgcc acctttgaca agctcctctg cctcagcctt ggccttggca
    14161 cgctcttcct cacggcgttc cttttcagtc agacggttaa agacggtaat cgcaccgatt
    14221 tccccaaaac caatggccgc aaagagggag tcttctgtct tgtaactggt cttttgcaga
    14281 acttgatcca tgtggcgctt gtccataaat ttatttgcca catagccatt ttcttggaac
    14341 tgagccatca gcatctcacg acccttgttg acagacaatt ccttatcttg gtttttaaag
    14401 aactggcgaa tcttattgcg cgccttgcta gtcttgacca tattgagcca gtcacggcta
    14461 ggtccaaagg agttcgggtt ggcgataatt tcaacctgat cccctgtctt taacttggtt
    14521 gtcagtggaa ccatgcggcc attgaccttg gcaccagttg ctttttcacc gaccttggta
    14581 tggatttcgt aggcaaaatc aatcggtcct gaatctttgg gaagggaacg gacagctcca
    14641 tctggggtaa aaacgtaaat ctcctcagcc agatagtttt ccttaacaga gtccacaaat
    14701 tccttagcat catcagcctg gtcttggagc tccatcatct ccttgatcca gttcattcca
    14761 atagctgatt ccttgctgtt aacttgcccc tttatacctt tcttataagc ccagtgagcc
    14821 gcaaccccgt actcagccac ctcgtgcatt tccttggttc gaatctggaa ttcaatcggc
    14881 ccttttggcc cataaacagt cgtatggata gactgataac cattggcctt gcggttggca
    14941 atatagtctt tgaagcgacc tggcatcggt ttccaaaatt catgcacgta accaagcatg
    15001 gcataaacat cactttgggt atctaaaata caacgaatag caatcagatc atagatttcc
    15061 tcaaaccgtt ttctcttgtc ctgcattttg cggaaaattg agtaaatatg cttgggacga
    15121 ccataaatct tccctttcaa gtgacgttct gtcgtatact cctctaattt tgtgactacc
    15181 tcatccacca aggcctcacg ctccctgcgc ttttccttca tcatatgggt aatcttgtaa
    15241 aactccgttg gattgagata acggaaagac aagtcttcta attcccattt gacactggaa
    15301 atccccaaac gatgggcaag tggggcatag atttccatgg tttctttgga aatacgctcc
    15361 tgcttgtctt ttcgaagatg tttcagggtc cgcatattgt gcaagcggtc agacagtttg
    15421 accaaaataa cgcggatgtc ctcagacatg gccatgagca tcttgcgatg attttccgct
    15481 aattgctcct cgattgattt gtactcgacc ttgccaagct tggtaactcc gtcaacaatc
    15541 acccgcacat caggaccaaa ctctctttcc aaatcgtcca aggtcgcatc tgtatcttcc
    15601 accacatcat gcaagaatcc acaagctact gttacagcat ccagctttag cttagctaaa
    15661 atacctgcca cttggatagg gtgaatgata taaggctcgc ctgatttgcg atattgacca
    15721 ctgtggcatt caacagcata gaccaaggcc ttatggacaa aatgaacatc ctcttccgtt
    15781 aaatattctt tggttaaagc gacaacttct tcgcctgtta aattcacttc tttcggcatc
    15841 tctactctcc aattcttcct accattttat cactttttta agaatatgaa aactagattg
    15901 gaacagaata agaaaaaaat aattcaaaat tgcttgataa ttctgaatta tcggcccata
    15961 atatactacg aagttagatt ttaaacttag gtgatagaag gagagataga agaacggaaa
    16021 ccatattgta acccaaagac tttctgattt ccccaattcc attgaagata cgaaagataa
    16081 acggtggaac tcgtatcaca tacactggta ccttgactgg attttggaat taatactcaa
    16141 tgaaaatcaa agagcaaact aggaaactag ccgcaggttg ctcaaagcac tgctttgagg
    16201 ttgtagataa gactgacaaa gtcagtcaca tatatacggc aaggcgacgt tgacgcggtt
    16261 tgaagagatt ttcgaagagt ataaaaatcc tcaagatact ttcttctatc ctttagttta
    16321 taaggagaat acctatgaaa aaaactgcta tttctatctt tgctctccta atgttaggag
    16381 tttgctgcct gttcctattc agccagcaaa gctataaaaa acagtcgttc aatactatgc
    16441 taacgaccag aacctgccca gtaggataac ttatagtgaa tatagcgaca aacgagaagc
    16501 caactacggt agcactctaa acatcacgtc tatcaaacaa gctaatgacg gagtttatgc
    16561 aacctatgaa gggcaattga cacctttcca atattgataa attgataacc agcctgtctt
    16621 catctagtca tgctggtttt taagttcatt ttaaatcctt acctattctc cctaactgtg
    16681 ctatacttaa tttatactca atgaaaatca aagagcaaac tagaaagcta gccgcaggct
    16741 gttcaaagca ctgctttgag gttgcagata aagttgacgc agtttgaaga gattttcgaa
    16801 gagtattagt acattctttg agattggagc tagtatgaaa atccataaaa ccgtgaatcc
    16861 tgttgcctat gaaaatacct attatctaga aggcgaaaag cacctcatcg tcgtcgatcc
    16921 tggtagtcat tgggaagcca ttcgtcagac aatcgagaag atcaacaaac cgatctgtgc
    16981 tattctcttg acccacgccc attatgacca tatcatgagt ctggacttgg ttcgcgagac
    17041 gtttggcaat cctcctgtct atatcgcaga gagcgaagcc agctggctct acactcctgt
    17101 cgataatctc tccggtctcc ctcgccacga tgatatggca gatgtggtca caaaacctgc
    17161 agaacacacc tttgtctttc acgaagaata ccaactagag gaatttcgtt ttaaggttct
    17221 accgacccca gggcactcta tcggtggtgt ttccctagtc tttcctgatg ctcacctagt
    17281 cttgacggga gatgctctat tccgcgaaac tatcggacgg accgaccttc cgactggtag
    17341 catggagcaa ctccttcata gtatccagac ccaactcttc accctaccaa actacgatgt
    17401 ctatccagga catggtccag ctactactat cgctcacgaa aaggccttca atcccttttt
    17461 ctagcaagat gatgacaatc gaaatttaag taaactatcc agcaaatctt tctattacaa
    17521 aaggcatcct atcaaggttt tcacacgtga ttggatgcct tttttctgat gactagattt
    17581 tttgtattac caaataatca cgcgctcctc tggtgaacgc cacattccgt ctccttcttt
    17641 gacatcatag gttgtaaaga aatcgtcgaa gtttggtact tgcacattga cacggagttt
    17701 ggctggtgcg tgcacatcga cgctagccaa aagtttcata aattctggtc gacctttcat
    17761 gcgccagatg cgaccgaagt tgtagaagaa ctcttctgct gagaagtctg cttctctctt
    17821 agctgcttca agcgctgctg cgattcctcc caagtcagcc acgttttctg atacagtcaa
    17881 tttaccgtta atggttgctc cataagaatc ctgtccatca aattggtcaa tgactttttg
    17941 tgttttctcc ttgaaggcag catagtcgct ctctgtccac caatccttga ggctaccatt
    18001 ttcgtcaaag gaagccccgt tagtatcaaa ggcgtgggaa atttcatggg caatcactgc
    18061 cccaatacca ccgtagttag cagaagatga ctgatgcaag tcatagaaag gcgcctgtaa
    18121 aatggccgct ggaaagacaa tcaggttctt ctgaggattg tagtaggcat tgaccatatg
    18181 agcaggcatg ccccattcct tatagtctac aggctggttc cacttactcc aactgtgctt
    18241 gatttccaca cgcgcaaagg ctagagcatt ctcaaaaaga ctggcagttt cattcactac
    18301 cttatccttg taacgtgcag gcaattcttc tggatagcca atataaggtt tgatcacatt
    18361 gagcttcacg atagcctgtt tacaggtttc tggagtgagc cagtcattct taagcagacg
    18421 ctccttatag acatcaatca tggttgccac ttttttctcc acatccgcct tggcttctgg
    18481 agagaacttc tcacgggcgt accaaagccc cagggcttgc ttgaaaggtt cctgtgctag
    18541 atgataagct gctttgacct tatcttttgc ctctggaact ccagaaaggg cacggctgta
    18601 ggcaccagac aaaacacgga tatcctctgt taaatagctg gttgaaagat tgacaacact
    18661 caaaatcaag gttgctttaa ggagagacca ggcttcctca ctgtaaaatt gctctgctgc
    18721 ttgccagaaa cgttcctcgt ctacaataac cttgtctggt aattgcccaa taactgcttt
    18781 gaagaagtca tccaaaggta gggcaggcgc gaatttcttg aaatcttcgt aagaatatgg
    18841 atgatagagt ttagcatatt ctgaactttc ttcattagag agcaccactg ccgcaactcg
    18901 gcggtccaat tcaagtcttt tttctagcaa gtcttcaatt tcttcatcag agaaatcata
    18961 agccttgagg agatttgcgc tgctttcttt ccaaagagtc aagagctctt cgcgctgagg
    19021 atgttcttct gcatagtagg tcgtatctgg caagattgtg cttggagcgc tagcccatag
    19081 aacattgatt ctagcatcca taaagtctgg cgatacacca aaaggaagga agtttggttt
    19141 tcctgcaagc tcaaactctg ctagtttagc tgtaaaatcc gcaaaagttt ccaattcttg
    19201 gaattcttta aggagtggta agacaggtgt gataccgtca gcttctctct tgtcaaaatc
    19261 acgaactagg cggtggtatt tgacaaagtt ttccaagata gcatcctcag gcacttcttc
    19321 acctgctaac cacttgtctg ttgtcgccag catcaggtct tcaatttcct ggtctaaatc
    19381 aacaaaacct cctgtttgag acttatctgc tgggatttca gctgtctgtt gccattctcc
    19441 attgatagca tcataaaaat catcttgata acgtgtcatc ttgttctcgc tttcatttgt
    19501 atttgcattt atcttaacaa aaatcactag ggaatgcaat ta
//
LOCUS       DAVMGZ010000025        19278 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000025 DAVMGZ010000000
VERSION     DAVMGZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.026"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            249..2036
                     /locus_tag="VYQ93_000749"
     CDS             249..2036
                     /locus_tag="VYQ93_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV3335477.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVSRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHCYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2033..2509
                     /locus_tag="VYQ93_000750"
     CDS             2033..2509
                     /locus_tag="VYQ93_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV3335478.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2537..3442
                     /locus_tag="VYQ93_000751"
     CDS             2537..3442
                     /locus_tag="VYQ93_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV3335479.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3429..4244
                     /locus_tag="VYQ93_000752"
     CDS             3429..4244
                     /locus_tag="VYQ93_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV3335480.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4251..4655
                     /locus_tag="VYQ93_000753"
     CDS             4251..4655
                     /locus_tag="VYQ93_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3335481.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKIRAAESIVKVNDLLASFDDDEDE"
     gene            4949..6115
                     /locus_tag="VYQ93_000754"
     CDS             4949..6115
                     /locus_tag="VYQ93_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV3335482.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTAYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6231..7268
                     /locus_tag="VYQ93_000755"
     CDS             6231..7268
                     /locus_tag="VYQ93_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV3335483.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7475..8329
                     /locus_tag="VYQ93_000756"
     CDS             7475..8329
                     /locus_tag="VYQ93_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV3335484.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGIGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     RGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSKLEITDSDGI
                     VTRIRLAR"
     gene            8479..8715
                     /locus_tag="VYQ93_000757"
     CDS             8479..8715
                     /locus_tag="VYQ93_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV3335485.1"
                     /translation="MTNSKYITRLKRSEGQLRGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAI"
     gene            complement(8949..9032)
                     /locus_tag="VYQ93_000758"
                     /pseudo
     CDS             complement(8949..9032)
                     /locus_tag="VYQ93_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
     gene            complement(9066..9158)
                     /locus_tag="VYQ93_000759"
                     /pseudo
     CDS             complement(9066..9158)
                     /locus_tag="VYQ93_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9194..9490)
                     /locus_tag="VYQ93_000760"
     CDS             complement(9194..9490)
                     /locus_tag="VYQ93_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV3335486.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNTWPGQVE"
     gene            complement(9480..9641)
                     /locus_tag="VYQ93_000761"
     CDS             complement(9480..9641)
                     /locus_tag="VYQ93_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV3335487.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTVQNLDGAYSLYKMA
                     NPEGVEYGN"
     gene            9711..10475
                     /locus_tag="VYQ93_000762"
     CDS             9711..10475
                     /locus_tag="VYQ93_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV3335488.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            11088..12467
                     /locus_tag="VYQ93_000763"
     CDS             11088..12467
                     /locus_tag="VYQ93_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV3335489.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            12481..13146
                     /locus_tag="VYQ93_000764"
     CDS             12481..13146
                     /locus_tag="VYQ93_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV3335490.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            13456..13994
                     /locus_tag="VYQ93_000765"
                     /pseudo
     CDS             13456..13994
                     /locus_tag="VYQ93_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            14094..14471
                     /locus_tag="VYQ93_000766"
     CDS             14094..14471
                     /locus_tag="VYQ93_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV3335491.1"
                     /translation="MIRREDQSVNEQVMIKCIDQQLKVNRLLVDQLDLSQVSHPKMRE
                     YLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQENPKVFQAIRKTMLSRLTKH
                     SVLPDRKLSNVVYQITKSVYGFN"
     gene            14587..16248
                     /locus_tag="VYQ93_000767"
     CDS             14587..16248
                     /locus_tag="VYQ93_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PavB family fibronectin-binding SSURE repeat
                     adhesin"
                     /protein_id="HEV3335492.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSRLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALD
                     GNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETS
                     DTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDT
                     MMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
     gene            16415..17113
                     /locus_tag="VYQ93_000768"
     CDS             16415..17113
                     /locus_tag="VYQ93_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3335493.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            17110..18162
                     /locus_tag="VYQ93_000769"
     CDS             17110..18162
                     /locus_tag="VYQ93_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV3335494.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            18357..18968
                     /gene="rpsD"
                     /locus_tag="VYQ93_000770"
     CDS             18357..18968
                     /gene="rpsD"
                     /locus_tag="VYQ93_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV3335495.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            19154..>19278
                     /locus_tag="VYQ93_000771"
     CDS             19154..>19278
                     /locus_tag="VYQ93_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3335496.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKL"
BASE COUNT         5488 a         3668 c         4378 g         5744 t
ORIGIN      
        1 ttaagcgata tagggattgc tcatatttta tcaatgtatt ctatacaaaa ttattttagg
       61 tcctatattc cgaatttaaa gagcgtttga aatgggtcta aaaatgtttg aaactaattg
      121 aatgtgtgta taatagtaaa aatgcagtaa atatcccgac aatttctatt gacaattcaa
      181 acagattggt ttataattaa tataacaaca aatgaaagcg caaactttcg cggtcggaag
      241 gtagttttat gacacgattt gagatacgag atgatttcta tctcgatgga aaatcattta
      301 agattttatc tggtgccatt cattatttta gggttcctcc agaggattgg tatcattcgc
      361 tctataactt gaaggctctt ggttttaata cggtagagac ttatgttgct tggaatttac
      421 acgagcctcg tgaaggtgag tttcattttg aaggtgatct ggatttagag aaatttctcc
      481 aaatagcgca ggatttgggt ctctacgcaa ttgtgcgtcc gtctccattt atctgtgcgg
      541 aatgggaatt cggtggctta ccagcttggc tcttgaccaa gaacatgcga attcgctcat
      601 ccgacccagc atatatcgag gcagttggtc gctactatga tcagttattg ccaagactgg
      661 tgtctcgttt gttggacaat ggtggcaata ttctcatgat gcaggttgaa aatgagtatg
      721 gttcttacgg agaagataag gcttacctga gagcgattcg acagctaatg gaagagtgtg
      781 gcgtaacctg tcccctcttt acatcagatg gtccatggcg agctactctg aaagctggaa
      841 ccttaattga agaggacctc tttgtaacag gaaactttgg ttctaaggca ccttacaact
      901 tttcgcagat gcaggaattc tttgatgaac atggtaagaa atggccactc atgtgtatgg
      961 agttctggga tggttggttc aatcgctgga aagaaccgat tatcacacgg gatcctaagg
     1021 aattggcaga tgcagttcga gaagttttgg aacaaggctc catcaatctt tacatgttcc
     1081 acggtggtac aaactttggt ttcatgaatg gttgctcagc tcgaggaact ttggacctgc
     1141 cacaagttac gtcttatgat tacgatgccc ttctggatga agaaggaaat ccaactgcta
     1201 aatatcttgc agtcaagaag atgatggcaa cacatttttc agagtatccg cagttggaac
     1261 cactctacaa agagagtatg gagttggatg ctattccact agttgaaaaa gtttctttgt
     1321 ttgaaacctt agatagcttg tcaagtcctg tagaaagtct ctatcctcaa aagatggagg
     1381 agctgggaca aagttatggc tacctacttt atcgaacaga aacaaactgg gatgcagaag
     1441 aagaaagact tcgtatcatt gatggtcgag atagggccca gctgtatgtc gatggtcagt
     1501 gggttaaaac tcaatatcag acagagattg gggaagatat tttttatcaa ggtaaaaaga
     1561 aagggctatc taggttagat atcttgatag aaaatatggg gcgtgtcaac tatgggcata
     1621 agttcttagc ggatacgcaa cgtaagggaa ttcggacagg ggtctgtaag gatctgcatt
     1681 tcttactaaa ctggaaacac tatccactcc cactagacaa tcctgagaaa attgattttt
     1741 caaaaggatg gactcaagga caaccagcct tttacgctta tgactttaca gtcgaagagc
     1801 caaaagatac ttacctagac ttgtctgagt ttggtaaggg ggttgccttt gtcaatgggc
     1861 agaatctagg acgtttttgg aacgttggcc caactctctc actttatatc cctcattgct
     1921 atctcaagga aggtgccaac cgcatcatta tctttgaaac agaaggtcaa tataaagaag
     1981 agattcattt aactcgtaaa cctacactaa aacatataaa gggggaaaac ttatgacaat
     2041 tgtaggatgc cgtattgatg gacgtttgat ccacggacaa gtagccaatc tttgggctgg
     2101 aaaactaaat gtttcacgca ttatggttgt agacgacgaa gttgtcaaca acgatattga
     2161 aaagagtggt ttgaaacttg cgacaccacc aggtgtgaaa ttgagtattt tgccagttga
     2221 gaaagctgca gccaatattc ttgctggcaa atacgatagc caacgtctct ttatcgtggc
     2281 tcgtaaacca gaccgcttcc ttggtttggt agaagcaggt gtaccacttg aaacccttaa
     2341 tgttgggaat atgtctcaaa caccagaaac tcgttctatt acacgttcta tcaacgtagt
     2401 agacaaggat gtggaagact tccacaaact ggcagaaaaa ggtgttaaac ttactgctca
     2461 gatggttcca aatgatccaa tttcagactt tttgagctta ttaaaatagg aaaaaatttt
     2521 ttaggaggtc attgttatga tacaatggtg gcaaatttta cttctcactt tgtactcagc
     2581 ttatcaaatc tgtgatgagt tgacgatcgt ttcatctgca ggttcccctg tatttgctgg
     2641 tttcattact ggtttaatca tgggagatgt gactactggt ttacttatcg gtggtaactt
     2701 gcaactgttc gttcttgggg ttggtacctt cggtggtgct tctcgtatcg acgcaacttc
     2761 tggtgcggtt cttgcgacag ccttctctgt ttcacaagga attgatgcac cgcttgccat
     2821 tactacaatc gctgtaccag tagcagctct cttgacttac ttcgacgttc ttggtcgtat
     2881 gactactacc ttcttcgctc accgtgtgga tgctgcaatc gaacgctttg actataaagg
     2941 tattgaacgc aactacttgc ttggtgcgat tccgtgggct ctatctcgtg cccttccagt
     3001 cttctttgcc cttgcttttg gtggtgcctt tgtacaatca gtagtagact tcgttgaagc
     3061 ctacaaatgg gttgcagatg gcttgacact tgcaggacgt atgcttccag gtcttggatt
     3121 tgcaatcttg cttcgttacc ttccagttaa acgtaacctt cactaccttg ctatgggatt
     3181 tggtttgaca gctatgttga ctgttcttta ctcatatgta acaggtcttg gtggcgctgt
     3241 tgctggtatc gtaggtactc ttcctgctga agttgctgaa aaaattggtt tcgtgaacaa
     3301 cttcaaaggt ttgtctatga ttggtatttc tatcgtaggt attttccttg cagtgcttca
     3361 cttcaaaaat agccaaaaag tagctgtagc agcaccttct acaccatcag aaagtgggga
     3421 aatcgaagat gacgaattct aattacaaac ttacaaaaga agattttaat caaatcaaca
     3481 aacgtagctt gtttactttc caattaggtt ggaactacga acgtatgcaa gcttctggtt
     3541 acctttacat gatcttgcct cagttgcgta aaatgtatgg tgatggaact cctgaattga
     3601 aagaaatgat gaaagttcat actcaattct tcaatacttc accattcttc cataccatta
     3661 tcgctggttt tgaccttgcc atggaagaaa aagatggtgt aggttcaaaa gacgccgtta
     3721 acggtatcaa gacaggtttg atgggaccat tcgctcctct tggggataca atctttggtt
     3781 cacttgtacc tgctatcatg gggtcagtcg cagcaactat ggctatcgct ggccaacctt
     3841 gggggatctt cctttggatt gcagttgcag tagcgtatga catcttccgt tggaaacagt
     3901 tggaatttgc ttacaaagaa ggggttaacc ttatcaacaa catgcaaagt accttgacag
     3961 ctttgattga cgctgcatct gtacttggtg tcttcatgat gggtgctctt gtagcaacag
     4021 tgattaactt tgaaatttct tacaagttgc caatcggtga aaagatgatt gatttccaag
     4081 acatcttgaa ccaaatcttc ccacgtttgc ttccagcaat ctttactgcc tttatcttct
     4141 ggttgcttgg taagaaaggt atgaactcta ctaaagctat cggtattatt atcgtacttg
     4201 ctttggctct ttctgccctt ggtcactttg cacttggaat gtaattcctt atgactaaat
     4261 cattaatttt ggtgagccat ggtcgcttct gtgaggagct tagaggtagt acagaaatga
     4321 ttatgggccc acaagacaac atttacacag tagctcttct tccagaagat ggcccagaag
     4381 aatttactgc taaatttgaa gctgttattg aaggattgga tgatttccta gtctttgcgg
     4441 atcttctcgg tgggacacct tgtaatgtgg tgagtcgctt gatcatggaa ggtcgtgata
     4501 ttgaccttta cgcagggatg aatcttccaa tggtgattga atttatcaat gcgagcctta
     4561 caggcgcaga tgcggactac aagatccgtg ctgcagaaag cattgtgaaa gttaatgacc
     4621 tgttagcgag cttcgatgat gacgaagatg aataatactc ttcgaaaaac tcttcaaact
     4681 acgtcaacgt cgccttgccg taggtatatg ttactgactt cgtcagtctt atccggcaac
     4741 ctcaaaacgg tgttttgagc tgacttcgtc agttctatct gcaacctcaa agcagtgctt
     4801 tgagcagcct gcggctagtt tcctatttta gttggaactc gattcaattc atgtgacaac
     4861 gtgaaaatcg ttagagcatt ttatatagaa tatacatggg aatgtagctt actctcattc
     4921 ccatatttaa tagaaaaaga ggaactcaat gctacattat acaaaagaag acttgctcga
     4981 attgggtgca gaaatcacta cgcgtgaaat ctaccaacag cctgatgtat ggagagaagc
     5041 ttttgaattt tatcaagcaa aacgtgaaga aattgcagcc ttcctacaag aaatcgctga
     5101 taaacatgac tatattaagg ttatcttgac aggtgctggg acttctgctt atgtgggaga
     5161 taccttgcta ccttatttta aggaagtcta tgacgaacgc aaatggaatt tcaatgctat
     5221 tgcgacaaca gatatcgttg ccaatccagc aacctatttg aaaaaagatg tggcaactgt
     5281 ccttgtgtct tttgctcgta gtgggaactc gcctgaaagt gtggcgactg ttgatttggc
     5341 caaatccttg gtggatgagc tttatcaagt gacgattact tgtgcagcag atggtaaatt
     5401 ggctcttcaa gctcacggtg atgaccgtaa tctcttgctc ttgcaaccag ctgtctctaa
     5461 tgatgctgga tttgccatga cttctagctt tacgtctatg atgttgacag ctctcttggt
     5521 ctttgatcct acagaatttg ctgttaagtc tgaacgtttt gaagttgtat ctagtcttgc
     5581 ccgtaaagtt ttagacaagg tagaagatgt caaagagctc gttgatttag actttaaccg
     5641 tgtcatctat ctaggcgctg gtcctttctt tggacttgct catgaagctc agctcaagat
     5701 tttggaatta actgctggtc aagttgcgac catgtatgaa agcccagttg gcttccgtca
     5761 cggtccaaaa tctcttatca acgacaatac agttgttttg gtctttggta caacgacagc
     5821 ctacactcgt aagtacgact tggacttggt tcgtgaagtt gctggtgacc agattgctcg
     5881 tcgtgttgtg cttttgagtg atcaagcttt tggtcttgaa aatgtcaaag aagtggtcct
     5941 tggttgtggc ggtgtcttga atgatattta ccgtgtcttc ccttacatcg tttatgccca
     6001 actctttgct ctattgactt cactcaaggt agaaaataaa ccagatacac cgtctcctac
     6061 aggtacagta aaccgtgtag tacaaggtgt cataattcac gaatatcaaa agtaagacag
     6121 tgtttatgaa ttcttgacaa gaggatttgt aaattatcag ataaaccata gattgtcagt
     6181 acgctttcta tggtttgttt gcttgagaga aatagtaaaa ggagaacaga atgaaagcat
     6241 acacagagcg tgtatttgga aatgttgagg gcgaggatgt cttggcctat cgatttgaga
     6301 cagacggtgg ctaccaactt gaggttatga cttatggtgc gaccatcttg cgctatgtcg
     6361 cacctgacaa ggctggaaat tttgccaatg ttatcttggg atttgatgac tttgatagtt
     6421 atgtaggcaa tagtcccaag catggagcaa gtgtaggtcc tgtagcgggt cgtattgcag
     6481 gtgcgacctt tgagctcaat ggtaagacct atgaccttga ggttaataat gctagcaact
     6541 gtaatcacag tggttcaact ggttgggatt ccagcttgtt tgaagttgaa gaagtaagcg
     6601 atcatggctt gactctctac acagagcgta cagatgggac aggagggttc cctggaaatc
     6661 tcaagatttg gatcagttat cacttggaag aaactggtgc ctatgaaatc agctacaagg
     6721 taacgaccga tcaggatacg ctggtcaatc caaccaacca cagctatttc aacttgtctg
     6781 gtgatttcac gcagacgatt gaccgtcatg tcttccaact aaacacagag ggcatttact
     6841 caatcgctcc tgacggtgtt cctgccaaaa ctccagaagc caaccgtgat gtggtcaaac
     6901 acatctacaa tggtgccttg ttgaaggata tctttgcaga agaagatgag caaatccagc
     6961 tggcatcagg tttggatcat ccatttgccc ttcctgcagg ccatgacaat gctggattcc
     7021 tttatgacca aaattcaggt cgcttcctgc ttttcaagac agaagctcct tgctttgtgg
     7081 tctacacagc aaactttgtg gatgaaagtg tcatcatagg aggtcagcca atgctacagc
     7141 acaatgggat tgctcttgaa gcgcaagctt taccagatgc cattcacagt gaccttaaag
     7201 gccaagtcat tcttaaagct ggtcaaacct tcaccagtaa gacacgttat gaacttgttg
     7261 tgaaataaaa aagctggtca aaaagtcttt aaaattaaaa taacgcatat tgtcaggtga
     7321 tgaaaagttt gataatatgc gttttattat gaaatgattt actttatatt atcctgaaat
     7381 ggtgttattt ttgagttatc tggcttgact ttcttgatgg aaattatata atagttgtaa
     7441 caataataat tacaacgaga aaagaggacc atttatgacc tatgaataca agagtcacat
     7501 ttatttggca gagacagttt taaatgtaaa ggatttggcc agtcaaacag ccttttatca
     7561 gcaagttatc ggtttagaaa tcttatctca aactgagaca gagtccattc taggcattgg
     7621 tggaaaagtc ttggtacagc tgattcaagc acaagagagc ggagaagtga gggaacatta
     7681 tggtctttac catttggcca ttctcttgcc cacacgcaag gctttggctg atgtcttgaa
     7741 acacctgaca gatttacaga ttcctcttgt cggcggtgca gaccatggtt acagtgaagc
     7801 tatttacttg gaagatttgg agggaaatgg cattgaactc tatcgagata agccagtttc
     7861 cacatgggat attcgagaag atggacgcat tatcggagtg acggaggctc ttgcggcgca
     7921 ggacatttat gagttggggg aaagagtaga gccctttatc ctggcagaag gtacgagaat
     7981 ggggcatatt catctttccg tcaaggatag tcgaaagtcc agccagtttt atcaaaaggt
     8041 gttagggcta gagaataaat tcagtgtgcc tggcgctagt tggattgcgg ctggagatta
     8101 ccatcatcat ttagcagtta acgaatggag aggaaaaggt ctggcttcgc gtaagcaagg
     8161 cttaccaggt ttagcctact atgtcatcga agtcgcacat aaaaaagaac tgttaacgat
     8221 tgcccaacga gcacaagaag ttgacgcacc aatcaaatgg atgacatcga gcaaattgga
     8281 aatcacagac tcagatggca tcgtgacccg tattcgttta gcaaggtaaa attaggaatt
     8341 tttaataagt tataagtatt gctagtttgc tttttgaacg gtctttggta acagaactgc
     8401 gtgagctgga tgggtgagaa ttttgacaaa gaagctgatt agagatataa tgaagaaaaa
     8461 tcgagcaagg aaaaagaaat gacaaattca aagtatatta ctcgtttgaa acgttcagag
     8521 ggccagttgc gtgggattca aaagatgatt gaaggagatc gtgactgtgc tgatattgtg
     8581 acgcaactga cagcagtgaa atctagtgta gagcgcgtga ttgagatgat aattaccgaa
     8641 aatcttactg aatgtatcaa tcagccccta gatgattctg aagctcaaaa ggaacgccta
     8701 gagaaggcta tctgatactt gattaaacgg aaataaatga atgttttgag acgatatgtg
     8761 caaaggtcta ttaactatta gaatagttta atgtaagtca aaagaagttg taaaaaatct
     8821 ttaaaattaa aaaacgcata gtatcaggta ttgaaatgta ctgcccccca aaagttagat
     8881 tttttctgtc taacttttgg ggggcagtac ataaaaacct tgatagtatg cgttttattg
     8941 tggggagact tatactcttc gaaaatctct tcaaaccacg tcagtttcac cttgccgtag
     9001 atatggttac tgacttcgtc agttctatcc acaacctcaa aacagtgttt tgagctgact
     9061 tcgtcagttc catccacaac cttaaaacag tgttttgagc agcctgcggc taacttccta
     9121 gtttgcactt taattttcat tgagtattac ttcatcttct catgaaattg agtttttgcc
     9181 cagctcctct tgattattct acttgaccgg gccaagtatt cataccacct tctacgttgg
     9241 taacggtgag gccttgggag ctgagaaatt ggcaggtaga tgcagagcgg actcctcctt
     9301 gacaaataac atagtattca tggtcaggtt tgagttcttt gtagccttgc tctaaggtac
     9361 ttaatggaag atttttggca cctggtgcat gtcctgcttg gaattcatgt gcttcacgga
     9421 catcgataag ttctagattt tcattttggt attttccata aaagtcagcc atgctgatac
     9481 tagtttccat attctactcc ttctgggtta gccattttgt atagtgaata agcgccgtca
     9541 aggttttgga cggtaaatcc tgcttgcttg aggataggct ctgcgatata gctgcgcaaa
     9601 ccactgtggc agctgacgat gtaggcttgt tttttgtcca gttcatcagc ttcctccttg
     9661 acactttgtc agtttttgat acaatagtac aaaattagag gaggtgggct atgattcaga
     9721 aacatgcgat tcccatttta gagtttgatg ataatcctca ggcggttatc atgcccaatc
     9781 acgaggggtt ggatttgcag ttgccaaaga agtgtgtcta tgctttttta ggtgaggaga
     9841 ttgaccgcta tgcgagggaa gtaggggcga actgtgttgg cgaattcgtt tctgctacca
     9901 agacctatcc agtctatgtc atcaactaca agggtgagga ggtctgtctg gctcaggctc
     9961 ctgttggctc cgctccagca gcccagttta tggattggtt gattggctat ggtgtggagc
    10021 agattatctc tactgggacc tgtggtgtcc tagctgatat agaggaaaat gcctttctag
    10081 tccctgttcg cgctttgcga gatgagggag ccagttacca ctatgtggca ccttgtcgtt
    10141 atatggaaat gcagccagag gctattgctg ctattgagga agttttggaa gacagaggga
    10201 ttccttatga agaagtcatg acctggacga cagacggttt ttaccgagaa acggctgaaa
    10261 aggtggctta tcgtaaggaa gaaggctgtg ctgttgtgga gatggagtgt tctgctcttg
    10321 cggcagtagc tcaattgcgt ggggttctct ggggtgaatt gttgttcaca gcagattctc
    10381 tagcggactt ggaccagtac gacagtcgtg actggggctc ggaagctttt aataaggcgc
    10441 tagaactgag tttagcaagt gttcaccacc tttagttgta ctggcaaagg atttgtttta
    10501 tcataaaatg tctagctcat acttttcaaa aatatgttta aacgaggtca ccttcctctt
    10561 gtcctaggca tgttgaggtt gggaaaaatc tttaaaatca gaaaaacgta tcatatcagg
    10621 tgatgaaaac tttgatacta tgcgttttat gttggtaaga tttagagtga gatgaaatga
    10681 tactcttcga aaatctcttc aaaccaggtc agcttcacct tgccgtaggt atatgttact
    10741 gacttcgtca gtcttatccg gcaacctcaa aacggtgttt tgagctgact tcgtcagttc
    10801 tatccacaac ctcaaaacag tgttttgagc aacctgtgac tagctttcta agcgatgcct
    10861 tggttctcat tgcctataat caaaaagaga aattttctcc tgaaaagcat atagagtagc
    10921 tggcgttaaa agctcctgtc ttgctttttt gacctatagt cacatctatc aagtattgtt
    10981 cttgcctaag ctatcaataa aaaggtggca ttttttaggc ttggtgttag tagattttgc
    11041 cttatcctat ctaagtcatt tcgagctttt tatggtacaa tggaaacatg ttattcaaat
    11101 tatctaagga aaaaatagag ctaggcttat ctcgtttatc gccagcccgt cgtatttttt
    11161 tgagttttgc cttggtcatt ttactaggct ctcttctttt gagcttgccc tttgtccaag
    11221 ttgaaagctc acgagcgact tattttgatc atcttttcac tgctgtctct gcagtctgtg
    11281 tgacaggtct ctcaaccctt ccagtagctc acacctataa tatatggggc caaataatct
    11341 gtttgctctt gattcagatc ggtggtctag ggctcatgac ctttattggg gttttctata
    11401 tccagagcaa gcaaaagctt agtcttcgta gccgtgcaac tattcaggat agttttagtt
    11461 atggagaaac tcgatctttg agaaagtttg tctattctat ttttctcacg acctttttgg
    11521 ttgagagctt gggagctatt ttgcttagtt ttcgccttat tcctcaactt ggctggggac
    11581 gtggtctttt tagttccatt tttctagcga tctcagcctt ctgtaatgcc ggttttgata
    11641 atttagggag caccagttta tttgcttttc agaccgattt actggtcaat ctggtgattg
    11701 caggcttgat tattacaggc ggccttggtt ttatggtctg gtttgatttg gctggtcatg
    11761 taggaagaaa gaaaaaagga cgtctgcact ttcatacgaa gcttgtatta ttattaacta
    11821 taggtttgtt gttatttgga acggcaacta ctctctttct tgagtggaac aatgctggaa
    11881 cgattggcaa tctccctgtt gccgataagg ttttagttag cttttttcaa acagtgacga
    11941 tgcgaacagc tggcttttct acgatagatt atactcaggc tcatcctgtg actcttttga
    12001 tttatatctt acagatgttt ctaggtgggg cacctggagg aacagctggg ggactcaaga
    12061 ttacgacatt ttttgtcctc ttggtctttg cacgaagtga gcttctaggc ttgcctcatg
    12121 ccaatgttgc gagacgaacg atcgcgccgc gaacggttca aaaatccttt agtgtcttta
    12181 ttatcttttt gatgagcttc ttgataggat tgattctgct agggataaca gccaaaggca
    12241 atcctccctt tatccacctc gtatttgaaa ccatttcagc tcttagtaca gttggtgtaa
    12301 cggcaaatct gactcctgac cttgggaaat tggctctcag tgttatcatg ccacttatgt
    12361 ttatgggacg aattggtccc ttgaccttgt ttgttagctt ggcagattac catccagaaa
    12421 agaaagatat gattcactat atgaaagcag atattagtat tggttaagaa aggaaagagc
    12481 atgtcagatc gtacgattgg aattttgggc ttgggaattt ttgggagcag tgtcctagct
    12541 gccctagcca agcaggatat gaatattatc gctattgatg accacgcaga gcgcatcaat
    12601 cagtttgagc cagttttggc gcgtggagtg attggtgaca tcacagatga agaattattg
    12661 agatcagcag ggattgatac ctgcgatacc gttgtagtcg cgacaggtga aaatctggag
    12721 tcgagtgtgc ttgcggttat gcactgtaag agtttggggg taccgactgt tattgctaag
    12781 gtcaaaagtc agaccgctaa gaaagtgcta gaaaagattg gagctgactc ggttatctcg
    12841 ccagagtatg aaatggggca gtctctagca cagaccattc ttttccataa tagtgttgat
    12901 gtctttcagt tggataaaaa tgtgtctatc gtggagatga aaattcctca gtcttgggca
    12961 ggtcaaagtc tgagtaaatt agacctccgt ggcaaataca atctgaatat tttgggtttc
    13021 cgagagcagg aaaattcccc attggatgtt gaatttggac cagatgacct cttgaaagca
    13081 gatacctata ttttggcagt catcaacaac cagtatttgg ataccctagt agcattgaat
    13141 tcgtaaagag ggatgacccc tcttttttga tgcccaagat ggcaaataga gacagaagcc
    13201 ccttgtcttc tagtaaaagt tcttcaaagg ctggacttta tggtaaaata gaaagaagtg
    13261 acaagagaga gtaatactca atgaaaatca aagatcaaac taggaaacta gctacgggct
    13321 gctcaaaaca gtgttttgag gttgcagata gaactgacga agtcagtaac atctatacgg
    13381 caaggcgacg ttgacgcggt ttgaagagat tttcgaagag tataagaaaa aatcagcccc
    13441 ctaaaggagc agattatgaa gttattgtct atcgccattt ctagctataa tgcagcagcc
    13501 tatcttcatt actgtgtgga gtcgctagtg attggtggtg agcaagttgg gattttgatt
    13561 atcaatgacg ggtctcagga tcagactcag gaaatcgctg agtgtttagc tagcaagtat
    13621 cctaatatcg ttagagccat ctatcaggaa aataaaggcc atggcggtgt ggtcaatcgc
    13681 ggcttggcag aggcttctgg gcgctatttt aaagtagttg acagtgatga ctggtggatc
    13741 ctcgtgccta cttgaaaatt cttgaaacct tgcaggaact tgagagcaag ggtcaagaga
    13801 tggatgtctt tgtgaccaat tttgtctatg aaaaggaagg tcagtcttgt aagaagagta
    13861 tgagttacga ttcagtcttg cctgttcggc agatttttgg ctgggaccag gtcggaaatt
    13921 tctccaaagg ccagtatatc atgatgcact cgctgattta tcggacagat ttgttgcgtg
    13981 ctagccagtt ctaattgcct gaacatactt tttatgtcga taatctcttt gtctttacgc
    14041 cccttcagca ggtcaagacc aggtactatc tgcctgtcga tttctatcgt tatttgatta
    14101 ggcgtgagga ccagtctgtc aatgagcaag tgatgattaa gtgcattgac cagcaactca
    14161 aggtcaatcg actcttggta gaccagcttg atttgtccca agtgagtcat cctaaaatgc
    14221 gagaatatct gctgaatcat attgaaatca cgacggtgat ttccagtacc ctgctcaacc
    14281 gatctgaaac agcggagcat ctggcaaaaa aacgccaatt gtggacctat attcagcagg
    14341 aaaatccaaa agtctttcag gctattcgta agaccatgtt gagccgtttg accaaacatt
    14401 ctgtcttgcc agatcgcaaa ctgtccaatg tcgtctatca aatcaccaaa tctgtttatg
    14461 gatttaatta atataagtgt tttataagag ggatttaaga aaaattttaa ctttttctta
    14521 gtccttttta atttcaggag attatactag agtcatcaaa taaagaaaga ctctaaggag
    14581 aatcctatga aattcaatcc aaatcaaaga tatactcgtt ggtctattcg ccgtctcagt
    14641 gtcggtgttg cctcagttgt tgtggctagt ggcttctttg tcctagttgg tcagccaagt
    14701 tctgtacgtg ccgatgtggt caatccgacc ccaggtcaag tcttacctga agagacatcg
    14761 ggaacgaaag agggtgactt atcagaaaaa ccaggagaca ccgttctcac tcaagcgaaa
    14821 cctgagggcg ttactggaaa tacgaattca cttccgacac ctacagaaag aactgaagtg
    14881 agcgaggaaa caaactcttc tagactggat acactttttg aaaaagatga agaagctcaa
    14941 aaaaatccag agctaacaga tgtcttaaaa gaaactgtag atacagctga tgtggatggg
    15001 acacaagcaa gtccagcaga aactactcct gaacaagtaa aaggtggagt gaaagaaaat
    15061 acaaaagaca gcatcgatgt tcctgctgct tatcttgaaa aagctgaagg gaaaggtcct
    15121 ttcactgccg gtgtaaacca agtaattcct tatgaactat tcgctggtga tggtatgtta
    15181 actcgtctat tactaaaagc ttcggataat gctccttggt ctgacaatgg tactgctaaa
    15241 aatcctgctt tacctcctct tgaaggatta acaaaaggga aatacttcta tgaagtagac
    15301 ttaaatggca atactgttgg taaacaaggt caagctttaa ttgatcaact tcgcgctaat
    15361 ggtactcaaa cttataaagc tactgttaaa gtttacggaa ataaagacgg taaagctgac
    15421 ttgactaatc tagttgctac taaaaatgta gacatcaaca tcaatggatt agttgctaaa
    15481 gaaacagttc aaaaagccgt tgcagacaac gttaaggaca gtatcgatgt tccagcagcc
    15541 tacttagaaa aagccaaggg tgaaggtcca ttcacagcag gtgtcaacca tgtgattcca
    15601 tacgaactct tcgcaggtga tggtatgttg actcgtctct tgctcaaggc atctgacaag
    15661 gcaccatggt cagataacgg tgacgctaaa aacccagccc tatctccact aggcgaaaac
    15721 gtgaagacca aaggtcaata cttctatcaa gtagccttgg acggaaatgt agctggcaaa
    15781 gaaaaacaag cgctcattga ccagttccga gcaaatggta ctcaaactta cagcgctaca
    15841 gtcaatgtct atggtaacaa agacggtaaa ccagatttgg acaacatcgt agcaactaaa
    15901 aaagtcacta ttaagataaa tgttaaagaa acatcagaca cagcaaatgg ttcattatca
    15961 ccttctaact ctggttctgg cgtgactccg atgaatcaca atcatgctac aggtactaca
    16021 gatagcatgc ctgctgacac catgacaagt tctaccaaca cgatggcagg tgaaaacatg
    16081 gctgcttctg ctaacaagat gtctgatacg atgatgtcag aggataaagc tatgctacca
    16141 aatactggtg agactcaaac atcaatggca agtattggtt tccttgggct tgcgcttgca
    16201 ggtttactcg gtggtctagg tttgaaaaac aaaaaagaag aaaactaatc agctaaggaa
    16261 ataaatgatg gatagtgggc tgactaagat tagtttaaca actcaatcag caatcaggac
    16321 tttctttcaa tagcagatta aaatcatcgt aaaacaataa aaatagtgtt atacttaaag
    16381 cagtatagca ctgtttttat caaaggagag acagatggga aagacaattt tactcgttga
    16441 cgacgaggta gaaatcacag atattcatca gagatactta attcaggcag gttatcaggt
    16501 cttggtagcc catgatggac tggaagcgct agagctgttc aagaaaaaac cgattgattt
    16561 gattatcaca gatgtcatga tgcctcggat ggatggttat gatttaatca gtgaggttca
    16621 atacttatca ccagagcagc ctttcctatt tattactgct aagaccagtg aacaggacaa
    16681 gatttacggc ctgagcttgg gagcagatga ttttattgct aagcctttta gcccacgtga
    16741 gctggttttg cgtgtccaca atattttgcg ccgccttcat cgtgggggcg aaacagagct
    16801 gatttccctt ggcaatctaa aaatgaatca tagtagtcat gaagttcaaa taggagaaga
    16861 aatgctggat ttaactgtta aatcatttga attgctgtgg attttagcta gcaatccaga
    16921 gcgagttttc tccaagacag acctctatga aaagatctgg aaagaagact acgtggatga
    16981 caccaatacc ttgaatgtgc atatccatgc tcttcgacag gagctggcaa aatatagtag
    17041 tgaccaaacg cccactatta agacagtttg ggggttggga tataagatag agaaaccgag
    17101 aggacaaaca tgaaactaaa aagttatatt ttggttggat atattatttc aaccctctta
    17161 accattttgg ttgttttttg ggctgttcaa aaaatgctga ttgcgaaagg cgagatttac
    17221 tttttgcttg ggatgaccat cgttgccagc cttgtcggtg ctgggattag tctctttctc
    17281 ctattgccag tctttacgtc gttgggcaaa ctcaaggagc atgccaagcg ggtagcggcc
    17341 aaggattttc cttcaaattt ggaggttcaa ggtcctgtag aatttcagca attagggcaa
    17401 acttttaatg agatgtccca tgatttgcag gtaagctttg attccttgga agaaagcgaa
    17461 cgagaaaagg gcttgatgat tgcccagttg tcgcatgata ttaagactcc tatcacttcg
    17521 atccaagcga cggtagaagg gattttggat gggattatca aggagtcgga gcaagctcat
    17581 tatctagcaa ccattggacg ccagacggag aggctcaata aactggttga ggagttgaat
    17641 tttttgaccc taaacacagc taggaatcag gtggaaacta ccagtaaaga cagtattttt
    17701 ctggacaagc tcttaattga gtgcatgagt gaatttcagt ttttgattga gcaggagaga
    17761 agagatgtcc acttgcaggt aatcccagag tctgcccgga ttgagggaga ttatgctaag
    17821 ctttctcgta tcttggtgaa tctggtcgat aacgctttta aatattctgc tccaggaacc
    17881 aagctggaag tggtggctaa gctggagaag gaccagcttt caatcagtgt gaccgatgaa
    17941 gggcagggta ttgccccaga ggatttggaa aatattttca aacgtcttta tcgtgtcgaa
    18001 acttcgcgta acatgaagac aggtggtcat ggattaggac ttgcgattgc gcgtgaattg
    18061 gcccatcaat tgggtgggga aatcacagtc agcagccagt acggtctagg aagtaccttt
    18121 accctcgttc tcaacctctc tggtagtgaa aataaagcct aaaacccctt tacaaatcca
    18181 gctattcatg gtagaataga ttttgtgtga aatatcagca ggaaagcatg aagctcgtca
    18241 acaggtgtct tatgacaagt aaccttggct gtttaggcga agggcatctg cacgaatcag
    18301 ggctttctaa gtgactattt ccaccgaaat attatttata tcaggaggac attcatatgt
    18361 cacgttatac aggaccatct tggaaacaag ctcgtcgcct tggcctttca cttacaggta
    18421 caggtaaaga attggcacgt cgtaactacg taccaggaca acacggacca aacaaccgtt
    18481 ctaaattgtc agaatacggt ttgcaattgg ctgaaaaaca aaaacttcgt ttcacttacg
    18541 gtgtaggtga aaaacaattc cgtaacttgt tcgtacaagc tacaaaaatc aaaggcggaa
    18601 tcctaggttt caactttatg cttcttttgg aacgtcgttt ggataacgtt gtttaccgtc
    18661 ttggtctcgc gactactcgt cgtcaagctc gtcaattcgt aaaccacggt cacatccttg
    18721 ttgacgggaa acgcgttgat atcccatcat accgcgtaac tccaggtcaa gtgatctcag
    18781 ttcgtgaaaa atcattgaaa gttccagcaa tccttgaagc agtagaagct actcttggac
    18841 gtccagcatt cgtatcattc gacgctgaaa agttggaagg ttcattgact cgcttgccag
    18901 aacgcgacga aatcaaccca gaaatcaacg aagcacttgt cgttgaattc tacaacaaaa
    18961 tgttgtaata ttttattgaa taagatagac tttgaggcct tgatattaag cactttggga
    19021 cgttctccct tagtgctttt ttgatttctc ttagtatcca gctataatcg ttgagacata
    19081 actagaccga tatagtccaa agtgatatag taaaatgaac caaaaatagt acacaatgtg
    19141 gtataatcct tttatggcat attcaataga ttttcgtaaa aaagttctct cttattgtga
    19201 gcgaacaggt agtataacag aagcatcaca cgttttccaa atctcacgta ataccattta
    19261 tggctggtta aagctaaa
//
LOCUS       DAVMGZ010000026        18239 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000026 DAVMGZ010000000
VERSION     DAVMGZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18239
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.027"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            59..364
                     /locus_tag="VYQ93_000772"
     CDS             59..364
                     /locus_tag="VYQ93_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3335497.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            412..1887
                     /locus_tag="VYQ93_000773"
     CDS             412..1887
                     /locus_tag="VYQ93_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV3335498.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2237..8938
                     /locus_tag="VYQ93_000774"
     CDS             2237..8938
                     /locus_tag="VYQ93_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV3335499.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKNLTVEWEEVPAEN
                     LIKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9186..10042)
                     /locus_tag="VYQ93_000775"
                     /pseudo
     CDS             complement(9186..10042)
                     /locus_tag="VYQ93_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10253..10612)
                     /locus_tag="VYQ93_000776"
     CDS             complement(10253..10612)
                     /locus_tag="VYQ93_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV3335500.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            10704..11660
                     /locus_tag="VYQ93_000777"
                     /pseudo
     CDS             10704..11660
                     /locus_tag="VYQ93_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11644..12201
                     /locus_tag="VYQ93_000778"
     CDS             11644..12201
                     /locus_tag="VYQ93_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV3335501.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12194..12448
                     /locus_tag="VYQ93_000779"
     CDS             12194..12448
                     /locus_tag="VYQ93_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335502.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12460..14514
                     /locus_tag="VYQ93_000780"
     CDS             12460..14514
                     /locus_tag="VYQ93_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV3335503.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLSLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            14693..15553
                     /locus_tag="VYQ93_000781"
     CDS             14693..15553
                     /locus_tag="VYQ93_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV3335504.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLRDPVFKKD"
     gene            15764..16474
                     /gene="ccdA2"
                     /locus_tag="VYQ93_000782"
     CDS             15764..16474
                     /gene="ccdA2"
                     /locus_tag="VYQ93_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV3335505.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16494..17060
                     /locus_tag="VYQ93_000783"
     CDS             16494..17060
                     /locus_tag="VYQ93_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV3335506.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17071..18183
                     /gene="msrB"
                     /locus_tag="VYQ93_000784"
     CDS             17071..18183
                     /gene="msrB"
                     /locus_tag="VYQ93_000784"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV3335507.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISEDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT         5505 a         3469 c         4005 g         5260 t
ORIGIN      
        1 attgctgaag caaccgctta atcttgtcta ttaaaaaata aaatcggagg aaatccaaat
       61 gattaaaatt cttgctgcct gcggtgcagg tgttaactca agtcaccaaa ttaaaagtgc
      121 tctagaagaa gaactttcaa accgtggtta tgatgttcac tgtgatgcag tcatggtgaa
      181 agatgtaaac gaagacctta tgaaaggtta cgatatcttt acaccaatcg ctgcaacaga
      241 ccttggtttt gaaccaggta tcccagttat cgaagctggg ccaatcttat tccgtatccc
      301 agcaatgagc gctccagtat ttgacaatat tgaagcagct attaaagaac atggattaag
      361 ctaattctaa tttgttaaca ttcatataac ataaaaaagg gaggaactgt tatggatgca
      421 atctttgacc taatcggaaa ggttttcaat cccatcttag aaatgggtgg acctgtcatc
      481 atgttgatca ttttgacagt attggcttta ctttttggag tgaaattctc caaagcgctt
      541 gaaggtggta tcaaacttgc catcgctctt acaggtatcg gtgctatcat cggtatgcta
      601 aatggtgctt tctcagcatc acttgcaaaa ttcgttgaaa acactggtat ccaattgagt
      661 attacagacg ttggttgggc accactcgca acaatcactt ggggttctgc ttggacacta
      721 tacttcttgc tcatcatgtt gattgtcaac gtagtgatgc ttgctatgaa gaaaaccgat
      781 acacttgatg tcgatatctt tgatatctgg cacttgtcta tcacaggtct cttgattaaa
      841 tggtatgctg ataacaatgg tgtgagtcaa ggggtttcac tctttattgc tacagcagct
      901 atcgtccttg tcggtgtgtt gaaaattatc aactctgact tgatgaaacc tacatttgat
      961 gaccttctta acgccccaag ttcatcacca atgacatcaa ctcacatgaa ctacatgatg
     1021 aacccagtta tcatggtttt ggataagatt tttgaaaaat tcttcccagg ccttgataaa
     1081 tatgactttg atgctgctaa attgaacaag aaaatcggtt tctggggatc taaattcttc
     1141 atcggtttca tccttggtat cgttatcggt attatgggaa ctccacatcc aattgcaggt
     1201 gttgcagatg cagataaatg gcgtcttgtt atcaaaggat ggttgtctct tggtttgact
     1261 gccggtgtat ctttggaact cttctcactt atcggttcat ggttcatcgc agccgtagaa
     1321 ccactatcac aaggtattac aaacgttgct actaaacgtc ttcgaggacg taaattcaat
     1381 atcggtcttg actggccatt catcgctggt cgtgctgaaa tctgggcttg tgccaacgta
     1441 cttgcaccaa tcatgttgat tgaagcagtg cttctttcaa aagttggaaa tggtatcttg
     1501 ccacttgcag gtatcatcgc tatgggtgtt actccagctc tcttggttgt aactcgtggt
     1561 aaattgctcc gtatgattat cttcggaaca ctcttgttgc cactcttcct tctttcaggt
     1621 acacttattg caccatttgc aacagaactt gctaaaggtg taggtgcctt cccagaaggt
     1681 gtgagccaaa ctcaattgat tactcactct actcttgaag gaccaatcga aaaacttctt
     1741 ggttggacaa ttggtaacac tacaactggt gatatcaaag caatccttgg tgcagtagtc
     1801 ttccttgtat tctatatcgg tatctttgct tggtacagaa aacaaatgat caaacgtaac
     1861 gaagagtacg cagcaaaagc aaaataatgc gctcctataa aatataaact caatgaaaat
     1921 caaagagcaa actaggaaac tagccgcagg ttgctaaaaa cacagttttg aggttgtaga
     1981 taagactgac gaagtcagct caaaacatgg ttttgaggtt gtagatgaaa ctgacgaagt
     2041 cagctcaaaa catggttttg aggttgtaga tgaaactgac gaagtcagta atcatatcta
     2101 cggcaaggcg acgctgacga agtttgaatt ggattttcgc agagtgtaaa ttgttgaaac
     2161 taggttgtca taccatgtat accacttggg ctttggtagt agctaactgc actaaatata
     2221 atataaggag ggttttatgg ggaaaggcca ttggaatcgg aaaagagttt atagcattcg
     2281 taagtttgct gtgggagctt gctcagtaat gattgggact tgtgcagttc tattaggagg
     2341 aaatatagct ggagaatctg tagtttatgc ggatgaaaca cttattactc atactgctga
     2401 gaaacctaaa gaggaaaaaa tgatagtaga agaaaaggct gataaagctt tggaaactaa
     2461 aaatgtagtt gaaaggacag aacaaagtga acctagttca actgaggcta ttgcatctga
     2521 gaagaaagaa gatgaagccg taactccaaa agaggaaaaa gtgtctgcta aaccggaaga
     2581 aaaagctcca aggatagaat cacaagcttc aagtcaagaa aaaccgctca aggaagatgc
     2641 taaagctgta acaaatgaag aaatgaatca aatgattgaa gacaggaaag tggattttaa
     2701 tcaaaattgg cactttaaac tcaatgcaaa ttctaaggaa gccattaaac ctgatgcaga
     2761 cgtatctacg tggaaaaaat tagatttacc gtatgactgg agtatcttta acaatttcga
     2821 tcatgaatct cctgcacaaa atgaaggtgg acaactcaac ggtggggaag cttggtatcg
     2881 caagactttc aaactagatg aaaaagacct caagaaaaat gttcgcctta cttttgatgg
     2941 cgtctacatg gattctcaag tttatgtcaa tggtcagtta gtggggcatt atccaaatgg
     3001 ttataaccag ttctcatatg atatcaccaa ataccttcac aaagatggtc gtgagaatgt
     3061 gattgctgtc catgcagtca acaaacagcc aagtagccgt tggtattcag gaagtggtat
     3121 ctatcgtgat gtgactttac aagtgacaga taaggtgcat gttgagaaaa atgggacaac
     3181 tattttaaca ccaaaacttg aagaacaaca acatggcaag gttgaaactc atgtgaccag
     3241 caaaatcgtc aatacggacg acaaagacca tgaacttgta gccgaatatc aaatcgttga
     3301 acgaggtggt catgctgtaa caggcttagt tcgtacagcg agtcgtacct taaaagcaca
     3361 tgaatcaaca agcctagatg cgattttaga agttgaaaga ccaaaactct ggaccgtttt
     3421 aaatgacaaa cctgccttgt acgaattgat tacgcgtgtt taccgtgacg gtcaattggt
     3481 tgatgctaag aaggatttgt ttggttaccg ttactatcac tggactccaa atgaaggttt
     3541 ctctttgaat ggtgaacgta ttaaattcca tggagtatcc ttgcaccacg accatggggc
     3601 gcttggagca gaagaaaact ataaagcaga atatcgccgt ctcaaacaaa tgaaggagat
     3661 gggagttaac tctatccgta caacccacaa ccctgctagt gagcaaacct tgcaaatcgc
     3721 agcagaacta ggtttactcg ttcaggaaga ggcctttgat acgtggtatg gtggcaagaa
     3781 accttatgac tatggacgtt tctttgaaaa agatgccact cacccagaag ctcgaaaagg
     3841 tgaaaaatgg tctgattttg acctacgtac catggtcgaa agaggcaaaa acaaccctgc
     3901 tatcttcatg tggtcaattg gtaatgaaat aggtgaagct aatggtgatg cccactcttt
     3961 agcaactgtt aaacgtttgg tcaaggttat caaggatgtt gataagactc gctatgttac
     4021 catgggagca gataaattcc gtttcggcaa tggtagcgga gggcatgaga aaattgctga
     4081 tgaactcgat gctgttggat ttaactattc tgaagataat tacaaagccc ttagagctaa
     4141 gcatccaaaa tggttgattt acggttcaga aacatcatca gcaacccgta cacgaggaag
     4201 ttactatcgc cctgaacgtg aattgaaaca tagcaatgga cctgagcgta attatgaaca
     4261 gtcagattat ggaaatgatc gtgtgggttg ggggaaaaca gcaaccgctt catggacttt
     4321 tgaccgtgac aacgctggct atgctggaca gtttatctgg acaggtacgg actatattgg
     4381 tgaacctaca ccatggcaca accaaaatca aactcctgtt aagagctctt actttggtat
     4441 cgtagataca gccggcattc caaaacatga cttctatctc taccaaagcc aatgggtttc
     4501 tgttaagaag aaaccgatgg tacaccttct tcctcactgg aactgggaaa acaaagaatt
     4561 agcatccaaa gtagctgact cagaaggtaa gattccagtt cgtgcttatt cgaatgcttc
     4621 tagtgtagaa ttgttcttga atggaaaatc tcttggtctt aagactttca ataaaaaaca
     4681 aaccagcgat gggcggactt accaagaagg tgcaaatgct aatgaacttt atcttgaatg
     4741 gaaagttgcc tatcaaccag gtaccttgga agcaattgct cgtgatgaat ctggcaagga
     4801 aattgctcga gataagatta cgactgctgg taagccagcg gcagttcgtc ttattaagga
     4861 agaccatgcg attgcagcag atggaaaaga cttgacttac atctactatg aaattgttga
     4921 cagccagggg aatgtggttc caactgctaa taatctggtt cgcttccaat tgcatggcca
     4981 aggtcaactg gtcggtgtag ataacggaga acaagccagc cgtgaacgct ataaggcgca
     5041 agcagatggt tcttggattc gtaaagcatt taatggtaaa ggtgttgcca ttgtcaaatc
     5101 aactgaacaa gcagggaaat tcacccttac tgctcactct gatctcttga aatctagtca
     5161 agtcactgtc tttactggta agaaagaagg acaagaaaag actgttttgg ggacagaggt
     5221 gccaaaagta cagaccatta ttggagaggc acctgaaatg cctaccactg ttccgtttct
     5281 atacagtgat ggtagtcgtg cagaacgtcc tgtaacctgg tcttcagtag atgtgagcaa
     5341 gcctggtatt gtaacggtga aaggtatggc tgacggacga gaagtagaag ctcgtgtaga
     5401 agtgattgct cttaaatcag agctaccagt tgtgaaacgt attgctccaa atactgactt
     5461 gaattctgta gacaaatctg tttcctatgt tttgactgat ggaagtgtac aagagtacga
     5521 agtagatagc tgggagatta cggaagtaga taaagccaaa ctttcagtag ctggatcacg
     5581 tattcaaatg actggtcagt tagctggaga aactattcat gcaacccttg tggtagaaga
     5641 aggaaatgct gcagcacctg tagtgccaac tgttactgtt ggaggtgaag cagtaacagg
     5701 tcttactagt cgacaaccaa tgcaatatcg tactctatct tatggtgccc aattgccaga
     5761 agtcacagca agtgctgaaa atgctgatgt gacagttctt caagcaagcg cagcaaacgg
     5821 catgcgtgca agcatatttg ttcagcctaa agatggtggc cctcttcaaa cctatgcaat
     5881 tcaattccta gaagaagcac caaaaattgc tcacttgagc ttgcaagtgg aaaaagctga
     5941 cagtctcaaa gaagaccaaa ctgtcaaatt gtcggttcga gctcactatc aagatggaac
     6001 gcaagctgta ttaccagctg ataaagtaac cttctctaca agtggtgaag gggaagtcgc
     6061 aattcgtaaa ggaatgcttg agttgcataa gccaggagca gtcactctca aagcggaata
     6121 tgagggagct aaaggccaag ttgaactcac tatccaagcc aatactgaga agaagattgc
     6181 gcaatctatc cgtcctgtaa atgtagtcac agatttgcat caaaaaccta ctcttccaac
     6241 aacagtaacg gttgagtatg acaaaggttt ccctaaagct cacaaagtca cttggcaagc
     6301 tattccgaaa gaaaaactag actcctatca aacatttgaa gtactaggta aagttgaagg
     6361 aattgacctt gaagcgcgtg caaaagtctc tgtagaaggt atcgtttcag ttgaagaagt
     6421 cagtgtgaca actccaatcg cagaagcacc acaattacca gaaagtgttc ggacatatga
     6481 ttcaaatggt cacgtttcat cagctaaggt tgcatgggat gcgattcgtc cagagcaata
     6541 cgctaaggaa ggtgtcttta cagttaatgg tcgcttagaa ggtactcaat taacaactaa
     6601 acttcatgtt cgcgtatctg ctcaaactga gcaaggtgca aacatttctg accaatggac
     6661 cggttcagaa ttgccacttg cctttgcttc agactcaaat ccaagcgacc cagtttcaaa
     6721 tgttaatgac aagctcattt cctacaataa ccaaccagcc aatcgttgga caaactggaa
     6781 tcgtactaat ccagaagctt cagtcggtgt cctattcgga gattcaggta ttttgagcaa
     6841 acgttcagtt gataacttaa gcgttggctt ccatgaagac catggagttg gtgcaccgaa
     6901 gtcttatgtg attgagtatt atgttggtaa gactgtccca acagctccta aaaaccctag
     6961 ttttgttggt aatgaggacc atgtctttaa tgattctgcc aactggaaac cagttactaa
     7021 tctaaaagcc cctgctcaac tcaaggctgg agaaatgaac cactttagct ttgataaagt
     7081 tgaaacctat gctgttcgta ttcgcatggt gagagcagac aacaaactag gaacgtctat
     7141 cacagaggta caaatctttg cgaaacaagt tgcggcagcc aagcaaggac aaacaagaat
     7201 ccaagttgac ggtaaagact tagcaaactt caaccctgat ttgacagact actaccttga
     7261 gtctgtagat ggaaaagtta cggcagtcac agcaagtgtt agcaacaatg gcctcgctac
     7321 cgtcgttcca agcgttcgtg aaggtgagcc ggttcgtgtc atcgcgaaag ctgaaaatgg
     7381 cgacatctta ggagaatacc gtctgcactt cactaaggat aagaacttac tttctcataa
     7441 accagttgct gcggttaaac aagctcgctt gctacaagta ggtcaagcac ttgaattgcc
     7501 gactaaggtt ccagtttact tcacaggtaa agacggctac gaaacaaaaa acctgacagt
     7561 tgaatgggaa gaagttccag cggaaaatct gataaaagca ggtcaattta ccgttcgagg
     7621 ccatgtcctt ggtagtgatc ttgttgctga ggtcactgta cgagtgacag acaaactagg
     7681 cgaagctctt tcagataacc ctaactatga tgaaaacagt aaccaggcct ttgcttcagc
     7741 aaccaatgat attgacaaaa actctcatga ccgcgttgac tatctcaatg acggagatca
     7801 ttcagaaaat cgtcgttgga caaactggtc tccaacacca tcttctaatc cagaagtatc
     7861 agcgggtgtg atcttccgtg aaaatggtaa gattgtagaa cggactgttg ctcaagccaa
     7921 acttcacttc tttgcagata gtggtacgga tgcaccaact aaactcgttt tagaacgcta
     7981 tgtcggtcca gagtttgaag tgccaaccta ctattcaaac taccaagcct acgacgcaga
     8041 ccatccattc aacaatccag aaaattggga agctgtgcct tatcgtgcgg ataaagacat
     8101 cgcagctggt gatgaaatca acgtaacatt taaagctgtc aaagccaaag ctatgagatg
     8161 gcgtatggag cgtaaagcag ataagagcgg tgttgcgatg attgagatga ccttccttgc
     8221 accaagtgaa ttgcctcaag aaagcacgca atcgaagatt cttgtagatg gaaaagaact
     8281 tgctgatttc gctgaaaatc gtcaagacta tcaaattacc tataaaggtc aacggccaaa
     8341 agtctcagtt gaagaaaaca atcaagtagc ttcaactgtg gtagatagtg gagaagatag
     8401 ccttccagta cttgttcgcc tcgtttcaga aagtggaaaa caagtcaagg aataccgtat
     8461 ccagttgact aaggaaaaac cagtttctga gaagacagtt gctgctgtac aagaagatct
     8521 tccaaaactc gaatttgttg aaaaagattt ggcctacaag acagttgaga aaaaagattc
     8581 aacactgtat ctaggtgaaa ctcgtgtaga acaagaagga aaaactggta aagaacgtat
     8641 ctttacagcg attaatcctg atggaagtaa ggaagaaaaa ctccgtgaag tggtagaagc
     8701 tccgacagac cgcatcgtct tggttggaac caaaccagta gctcaagaag ctaaaaaacc
     8761 acaagtgtca gaaaaagcag atacaaaacc aattgattca agtgaagcta gtcaaactaa
     8821 taaagcccag ttaccaaata caggtagtgc ggcaagccaa gcagcagtag cagcaggttt
     8881 agctcttcta ggtttgagtg caggattagt agttactaaa ggtaaaaaag aagactagaa
     8941 attttgatac cttctttatc attaaagacc aaagaaaaac tcttgtccta aatagaagat
     9001 aaagagtctg gaacaaaata gttctcaacc acaaaaaagc tagatatttc caattgcgga
     9061 actctagctt tttaattttg agtcgttctc ttattgtata gtagattgaa actagagtag
     9121 tacacttcta cttctaaaac attgttagaa atcgatttga ctgtccttat cgatttgtcc
     9181 tgttcttatt tcatttcact atgttttagg aggttattgg ccataagtac caatcccatg
     9241 tcaattctca cttgacgctt gcctctcaga ttacatctct tgtaacccaa acaagccttt
     9301 atctgcccaa agacaggttc tacatcaatc ttgcgttgag cgaaaatctg tctatcttgg
     9361 ggagataaaa gcgcctgaca ttctttagct ttcaagtttt gatagcgttc gttcatatac
     9421 aatccctttt gaggggctga ttcaggttca tcagcgtagt aaaccttgat ttcctgttga
     9481 aagtctgtct gtgttttctg gtacttgata tggtgaaaac gatagcacca gccatcagga
     9541 tgtatgtagc tatcctcctt gtcattatag tgccaattcg ctaagttttt agctgactgt
     9601 ttataccctc tcttttgttc cttatcaaac atggcatatt taatcagaag gggagtgatt
     9661 tcttcttgaa aatagacttg tatctgttct tgaagtttgg cggagaattt ctctgtcgtt
     9721 ttcttccaca caaaactata cttgttggca ttggattcaa tcttagttcc gtcaataaac
     9781 aaacaatcta aggtcactaa ctcttccatt tttcaatttt tcgaccagag aaaatccctt
     9841 gtgaataggc aaatagaaga gtagatacaa gcattttagg gtgataagac gggcgaccaa
     9901 aggcatgata ggaggtgtag aagtgacgtt cctccaaggt attcaccact ttttcaatag
     9961 taaagacgag atggtcttgt ggtaagaagg aactgatttc tagtggtaaa gttgtttgat
    10021 ttgtgttata gtgaatatgc atgggatagc ctttctaaat ggtttattaa gcaattctat
    10081 tttaccaaga ctatcgtaac catgcaaaaa agcaacggca aatccgttgc cttttttgga
    10141 tataagagac tattgtccca gactcttttc tttgtataaa attgtatagt gttattgtct
    10201 atactatttt gtagagaaag caaaaatccc tagaaaatgc ttagggattc gactatttca
    10261 ttaagaacaa ttcaacaatc atctctatat acatgataag ggttaataaa aatcctgtac
    10321 gccagaagaa tttgatgaat ttaggatagt aaaagctgcg tttttttaaa aggaagaaaa
    10381 agacgagaat aatggctagg aacgagaggg ctagccccag tctagggagg aaattatggg
    10441 taaaggtttt agctgtaatc aagtaatact caaataccaa gactggaaaa gccaaatccg
    10501 caaagtttcg tcctagtttt tttaatctaa aaagtttggt taggataatg cagacgacta
    10561 aggtcagtat caataataaa atagatgcta atttcattaa aatcataccc atattgtatc
    10621 ataaaaaaag gctaatggaa acgagaaaca gaggaattta taggaaagtc aggcttttga
    10681 gattgtgggt gatttttgtt ataatgaaag ttataaaatc ttatgatacc ttgaatgatt
    10741 attatcgaaa actctttgga gaaaagactt ttaaagtccc tattgatgcg ggatttgact
    10801 gtcccaatcg tgatggaact gtggctcatg gaggctgtac tttttgtacg gtttccggtt
    10861 ctggagatgc cattgtagcc ccagatccgc ctatccgtga gcaattttat aaggaaattg
    10921 actttattca tcgtaagtgg ccagatgttc agaagtatct ggtttatttt caaaatttta
    10981 ccaacaccca tgaaaaggtg gaagttattc gagagcgtta tgaacaggct atcaatgagc
    11041 caggtgtggt aggaatcaat attggaacgc gcccagactg tttaccagac gaaaccatcg
    11101 aatatttggc tgagttatcg gagtgcatgc atgtgacggt tgaattgggc ttgcagacta
    11161 cttatgaagc aacctctgac ctgattaacc gtgcgcattc ttatgaattg taggtggaaa
    11221 cggtcaagcg tttgagaaag tatccaaaaa ttgagattgt ttcccatttg atcaatggtt
    11281 tgcctggtga aacccatgag atgatggttg aaaatgtccg ccgttgtgtc acggataacg
    11341 atattcaagg gattaaactg catttgctcc atcttatgac aaatactcgt atgcaacgag
    11401 attaccatga gggacgttta caattgatga gtcaggacga atatgtcagg gtcatctgtg
    11461 accaactgga aattattccc aaacatatcg tcatccatcg aattacagga gatgcgccta
    11521 gggatatgct gattggtcct atgtggagcc tcaataaatg ggaagtcctc aacagcattg
    11581 agatggagat gagacgtcgt ggaagtgttc aaggatgcaa ggctgtaaaa caggagtttg
    11641 aaaatgaaaa gaccacttga gatggcacat gattttttgg ctgaggtcgt gacaaaagag
    11701 gatgtcgtag tggatgcgac tatgggaaat ggtcatgaca cgcttttttt agccaagcta
    11761 gccaagcaag tctatgcctt tgatattcag aagcaagcct tggaaaagac ccaagagcgt
    11821 ttgcatcagg ctggcttgac aaatgcccag ttaatcttgc aaggccatga gacactggac
    11881 cagtttgtga caaaagctaa ggcagggatt tttaatttgg gctatttgcc gtcagctgat
    11941 aagtctgtca tcacccgacc gcagacaacg attgaggcat tagaaaagct atgtggctta
    12001 cttgtcaaag gtggacgaat tgctattatg atttactatg gtcatgaagg aggcgacctc
    12061 gagagagatg ctgtcttgga ttttgtgatc cagttgaacc aacaagagta cacagctgcc
    12121 atttaccgaa ctttaaacca agtcaacaac ccgccgtttt tagtgatgat tgaaaaatta
    12181 gagagataca gacatggata aacaatacct acacgaaaag ctggatgcca tgcgccagaa
    12241 ttttgttgaa tcaacctacc acgaacgagc ggtgggcgtg ctagaccaag cgcatatgag
    12301 caaaaaaatg cttaaaatca agaaaaaatt agttgctctt gaaatggaac ggtgccagag
    12361 aaaaattgag cacaaagact gttccaagat tgaccaaaaa atcaaagagc agaaggagat
    12421 atttgaatcc tgttgtaaaa aagattaagg aggacgtgcg tggaattact gatttacctc
    12481 atcctatttt tactggtctt gattgtctca agtacaacca ataagctcct accctttttg
    12541 cctctccctt tggtgcagat tcttttggga attgtgattg gtctcttttt acccaatact
    12601 gactttcacc ttaatacgga gttgtttttg gcactggtta tcggcccctt gcttttccga
    12661 gaggctgaag aagcagatgt tacggctatt ttaaaacact ggcgaatcat tgtttatctc
    12721 atatttccag tgatttttat ctcgaccctg agtttgggtg gcttggccca tcttctttgg
    12781 ttcagccttc ccttggcagc ttgcttggct gttggggcag cccttggtcc tacggacttg
    12841 gtggcctttg cctctctttc ggagcgtttt agctttccta agcgcgtgtc caatattctt
    12901 aagggcgaag gactcttgaa tgatgcttct ggtttggtgg cttttcaggt agctttgaca
    12961 gcttggacaa ctggagcttt ttctctgggg caagctagca gttcactcat cttttcaatc
    13021 ctaggcggtt ttttaattgg atttttaaca gccatgacca accgcttcct ccataccttc
    13081 ttgctaagtg tgcgtgcaac ggatattgcc agtgaacttt tattagaatt gagtttgcct
    13141 cttgtgacct tctttctggc agaagaagtt catgtttcag gtattattgc cgtcgtagtt
    13201 gctggaattt taaaggcaag tcgtttcaag aaaatcacgc tcctcgaagc ccaagtggat
    13261 acggtgaccg agacggtctg gcatacagtg acctttatgc tcaacggttc tgtctttgtg
    13321 attttaggga tggagctgga aatgatagca gaacctatct tgaccaatcc aatctataat
    13381 cctctacttt tattgctatc tcttatcgcc cttacctttg tcctctttgt cattcgtttt
    13441 attatgatct atggctatta tgcctataga acccgacgcc tcaagaaaaa gctaaataag
    13501 tatatgaagg acatgtttct cttgaccttt tcaggtgtta agggaacggt gtcgattgct
    13561 acgattctct tgataccaag taatctagaa caggagtatc ctctcttgct tttccttgtt
    13621 gcaggtgtga cgcttgttag ctttttaaca ggtctcttgg tcttgcctca tctttctgat
    13681 gaagaggaag aaagcaagga ttatctcatg catatcgcca ttttgaatga agtaacgcta
    13741 gagttggaaa aagagttgga agacaccaga aataaacttt ccctctatgc ggctattgac
    13801 aattatcatg gacgtattga aaatctcatt ttaagccaag aaaaccagga tgatcaagaa
    13861 gactgggctg ctttgaaact cttgattctt agtattgaaa gtgatggttt ggaacaggcc
    13921 tatgaagagg ggaatattag caatcgtgct taccgagttt accaacgtta tctgaaaaat
    13981 atagaacaag gaatcaatcg taaacttgcc tcaagactga cctattattt tcttgtttcc
    14041 ttgaggattt tacgttttct ccttcatgaa gtttttactc ttggaaagac cttccgtagc
    14101 tggaaggaca aggagcaaag ccgtctccgt gctcttgatt atgaccaaat tgcagagctc
    14161 tatcttgcca atacagagat gattattgaa agtttggaaa acctgaaggg agtctacaga
    14221 cgctctttga ttagttttat gcaggagtct cgtcttcgag aaacagctat tatcagcagt
    14281 ggtgcctttg tcgaacgggt tatcaatcgt gtcaaaccca acaatatcga tgaaatgctg
    14341 agaggctatt atctggagcg caagttgatt ttcgaatacg aagaaaaacg attaattaca
    14401 actaagtatg ccaagaaatt acgacaaaat gtaaataact tagagaacta ttccttgaag
    14461 gaagctgcca ataccctgcc gtatgatatg gtggaattgg taagaagaaa ttagttaata
    14521 ctcttcgaaa atctcttcaa accacgtcag cgtcgccttg gattatatat gtgactgact
    14581 tcgtcagttt catctacaac ctcaaagcag ggctttgagc aacctgcggc tagcttccta
    14641 gtttgctctt tgattttcat tgagtataag attataaatg aaggagtatg acatgaaaaa
    14701 gtggtggaaa gagctgatag acaagccttt attaaaagct tttttgcatt attatcaagc
    14761 atcagatagt gagttgacca gtgtcgcagt agcctactat tggttgattt cgattttccc
    14821 cctgcttttg gtggtggtca atatcctccc ctattttcag attcctgtgg gagaatttct
    14881 gtgctttatg aaagacgttc tgcccccaag cctctatgaa ggtgtggaaa aaatagccag
    14941 agaagttttg acgcaacctt caacaggttt attgagtttt tctgttttat cggcgttatg
    15001 gagtttttca aaatctatga atttcttgca aaaagctttt aacaaggctt atggcgtcga
    15061 aaagagtcgt ggacttattt cccatcagat gctaagtctc ctagtcagtt ttggcttgca
    15121 gctcctcttt gcgtttgcct tgtttttgat cttatttggt cagatgattt tggctttact
    15181 tgctcattat tggacaaaag atggcattat ctatcaggct ttacaagggt tggcaggtcc
    15241 tctgatttac gccctcctct ttgctatctt ggtcatgctt tattattttc ttcccaatat
    15301 atctaacagg aaaattagct ataccctacc aggcagtgcc tttgttctct tagtgatttt
    15361 aggtttacta actctgttct ctagttatct caattactat gtccatcatt tggtggatgt
    15421 ccgaatttta ggttccgttc ttcttgttgt tatgatgttt tggtttatct tgattgctaa
    15481 aattgttatc ctaggcgcgg ttatcaatgc cagtatgcag agtttgagag atccggtctt
    15541 taaaaaagac taactattta tccattacaa aagcgcatag tatcaaaact ttatagaacc
    15601 ttgatactat gcgcttttgt gctttgacag ttttttgtat agtttttgtc caagatttct
    15661 aaatcatctg attaaattcc tattttcccc tatcattgtc cctgtttttt cgaaaatgga
    15721 aggagtataa taaatctatc aataaaatga taggaggaac tttatgggtc atattttctt
    15781 ttttctaagt gtctttttgg cagggattct atccttcttt tctccttgta tcttaccttt
    15841 gttaccggtc tatacaggag tgttactaga tgataaggat ggtgctcagg cttctagcgg
    15901 caaattttca atctcagtta ctagtttatt acgaactctg gcctttatag caggaatttc
    15961 ctttatattt attttgttgg gctatggagc tggtttttta ggcgatttgc tttatgcttc
    16021 ttggttccaa tatcttactg gggcaattat tatccttctt ggtttgcacc aaatggagat
    16081 tctacacttt aaggggcttt ataaggaaaa gaggctacaa ctgcaaggac aggggcaaaa
    16141 tggtaagggc tatagtcagg catttttatt gggcttgacc tttagttttg cttggacgcc
    16201 ttgcgtgggg ccggttctgg ggtctgtttt ggccttggcg gcttcaggtg gttcaggagc
    16261 ttggcaggga gctggtctca tgttggtgta tacgctgggc ttggcgctac cattcttgct
    16321 tctagctctg acctctagtt atgttttgaa acatttccga aaacttcatc cctatctcgg
    16381 aatcctcaaa aaagtgggtg gttttctcat tattgtgatg ggactcttgg ttctgtttgg
    16441 aaatgcttca attttaagtc aattatttga ataaaatgga aaggaatatc aatatgaaaa
    16501 aatggcaaac atgtgttctt ggagcaggtt cgctcctttg tttgacggct tgttcaggca
    16561 agtccgtgac tagtgaacac caaacgaaag atgaaatgaa gacggagcag acagctagta
    16621 aaacaagcgc agctaaaggg aaagaggtgg ctgattttga attgatggga gtagatggca
    16681 agacctaccg tttatctgat tacaagggca agaaagtcta tctcaaattc tgggcttctt
    16741 ggtgttccat ctgtctggct agtcttccag atacggatga gattgctaaa gaagctggtg
    16801 atgactatgt ggtcttgaca gtagtgtcac caggacataa gggagagcaa tctgaagcgg
    16861 actttaagaa ttggtataag ggattggatt ataaaaatct cccagtccta gttgacccat
    16921 caggcaaact tttggaaact tatggtgtcc gttcttaccc aacccaagcc tttatagaca
    16981 aagaaggcaa gctggtcaaa acacatccag gattcatgga aaaagatgca attttgcaaa
    17041 ctttgaagga attagcctag gaggtgtctt atgaatgata agttaaaaat cttcttgttg
    17101 ctaggagtat tttttctagc cataaccggt ttctatgttc tattgatacg aaatgcaggg
    17161 cagacagatg cctcgcaaat tgaaaaggcg gcagttagcc aaggaggaaa agcagtgaaa
    17221 aaaacagaaa ttagtgaaga cgcagacttg cacgaaattt atctagctgg aggttgtttc
    17281 tggggagtgg aggaatattt ctcacgcgtt cccggggtga cggatgccgt ttcaggctat
    17341 gcaaatggta gaggagaaac aaccaagtac gaattaatta accaaacagg tcatgcagaa
    17401 accgtccatg tcacctatga tgccaagcaa atttctctca aggaaatcct gcttcactat
    17461 ttccgcatta tcaatccaac cagcaaaaat aaacaaggaa atgatgtggg gacccagtac
    17521 cgtactggtg tttattacac agatgacaag gatttggaag tgattaacca agtctttgat
    17581 gaggtggcta agaaatacga tcaacctcta gcagttgaaa aggaaaactt gaagaatttt
    17641 gtggtggctg aggattacca tcaagactat ctcaagaaaa atccaaatgg ctactgccat
    17701 atcaatgtta atcaggcggc ctatcctgtc attgatgcca gcaaatatcc aaaaccaagt
    17761 gatgaggaat tgaaaaagac cctgtcacct gaggagtatg cagttaccca ggaaaatcaa
    17821 acagaacgag ctttctcaaa ccgttactgg gataaatttg aatccggtat ctatgtggat
    17881 atagcaactg gggaacctct cttttcatca aaagacaaat ttgagtctgg ttgtggctgg
    17941 cctagtttta cccaacccat cagtccagat gttgtcacct acaaggaaga taagtcctac
    18001 aatatgacgc gtatggaagt gcggagccga gtaggagatt ctcaccttgg gcatgtcttt
    18061 acggatggtc cacaggacaa gggcggctta cgttactgta tcaatagcct ctctatccgc
    18121 tttattccca aagaccaaat ggaagaaaaa ggctacgctt atttactaga ttatgttgat
    18181 taatactctt cgaaaatctc ttcaaaccac gtcagcgtcg ccttaccgta tgtagggtt
//
LOCUS       DAVMGZ010000027        18060 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000027 DAVMGZ010000000
VERSION     DAVMGZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18060
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.028"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            68..5377
                     /locus_tag="VYQ93_000785"
     CDS             68..5377
                     /locus_tag="VYQ93_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV3335508.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPRAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDTDNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVAED
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDASTVEKPEFKLSSLASDEDKAPAPELKQE
                     VTKRETAEQSLPATGESQSDTALFLAGVSLALSALFVAKTKKD"
     gene            complement(5545..7710)
                     /locus_tag="VYQ93_000786"
     CDS             complement(5545..7710)
                     /locus_tag="VYQ93_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV3335509.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGSRHQSSNVSFGINQAVETNRDWGSAMKPITDYAPALEYDIYDSTAS
                     IVHDVPYNYPGTDTPLYNWDKVYFGNITIQYALQQSRNVTAVETLNKVGLDRAKTFLN
                     GLGIDYPSMHYANAISSNTTESNKKYGASSEKMAAAYAAFANGGIYHKPMYINKIVFS
                     DGSSKEYADPGTRAMKETTAYMMTEMMKTVLAYGTGRGAYLPWLPQAGKTGTSNYTDD
                     EIENYIKNTGYVAPDEMFVGYTRKYSMAVWTGYSNRLTPIVGDGFYVAAKVYRSMMTY
                     LSEDNNPGDWTMPEGLYRSGEFVFKKGARSAWTAPAPQQAPTPESSSSTSESSTSQSS
                     STTPSTNNSANNNTNNQQPNTTPGQQNQNQNQNPQPAQP"
     gene            complement(7707..8303)
                     /gene="recU"
                     /locus_tag="VYQ93_000787"
     CDS             complement(7707..8303)
                     /gene="recU"
                     /locus_tag="VYQ93_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV3335510.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            8369..8896
                     /locus_tag="VYQ93_000788"
     CDS             8369..8896
                     /locus_tag="VYQ93_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV3335511.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            8966..9295
                     /gene="gpsB"
                     /locus_tag="VYQ93_000789"
     CDS             8966..9295
                     /gene="gpsB"
                     /locus_tag="VYQ93_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV3335512.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            9316..9696
                     /gene="rnpB"
                     /locus_tag="VYQ93_000790"
     ncRNA           9316..9696
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYQ93_000790"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9781..10938
                     /locus_tag="VYQ93_000791"
     CDS             9781..10938
                     /locus_tag="VYQ93_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV3335513.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQIENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            10950..12344
                     /gene="mapZ"
                     /locus_tag="VYQ93_000792"
     CDS             10950..12344
                     /gene="mapZ"
                     /locus_tag="VYQ93_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV3335514.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSLSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            12420..13844
                     /gene="gndA"
                     /locus_tag="VYQ93_000793"
     CDS             12420..13844
                     /gene="gndA"
                     /locus_tag="VYQ93_000793"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV3335515.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVYASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            13856..14545
                     /locus_tag="VYQ93_000794"
     CDS             13856..14545
                     /locus_tag="VYQ93_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3335516.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            14643..15659
                     /gene="cbpC"
                     /locus_tag="VYQ93_000795"
     CDS             14643..15659
                     /gene="cbpC"
                     /locus_tag="VYQ93_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV3335517.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMATGWYQDGSTWYYLNASNGGMKTGWFQVNGKWYYAYGSGALAVNTTVDGYSVNYNG
                     DWVR"
     gene            complement(15778..17010)
                     /locus_tag="VYQ93_000796"
     CDS             complement(15778..17010)
                     /locus_tag="VYQ93_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3335518.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILYQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAYIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(17053..17640)
                     /locus_tag="VYQ93_000797"
     CDS             complement(17053..17640)
                     /locus_tag="VYQ93_000797"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335519.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCEIAY
                     ALFAYSKVLEPSMFYNDLHIFHTELKKLLEKVTSNTENLGNLQLSWCKGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKADLFDFGIGSMGYIGVY"
     gene            complement(17540..18016)
                     /locus_tag="VYQ93_000798"
     CDS             complement(17540..18016)
                     /locus_tag="VYQ93_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
                     /protein_id="HEV3335520.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYPPELDSKILSIIK
                     KAVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAKKLIENYDTL
                     EEIDFALGKSGVLLPLIKYYQFTNDNTLKTFIHNSIWGNLSLFPTKRYSQRKHFRL"
BASE COUNT         5602 a         3287 c         4099 g         5072 t
ORIGIN      
        1 gaggaaggag tattttttaa aaaggaaatg taaacgcttg ctaaaaaatg aaaggattta
       61 gaagtttatg aataaaggat tatttgaaaa acgttgtaaa tatagtattc ggaaattttc
      121 attaggtgtt gcttctgtta tgattggagc tgcattcttt gggacaagtc cggttcttgc
      181 agatagcgtg cagtctggtt ccacggcgaa cttaccagct gatttagcta ctgctcttgc
      241 aacagcaaaa gagaatgatg gacgtgattt tgaagcgcct aaggtgggag aagaccaagg
      301 ttctccagaa gttacagatg gacctaagac agaagaagaa ctattagcac ttgaaaaaga
      361 aaaaccggct gaagaaaaac caaaagagga taaacctgca gctgctaaac ctgaaacacc
      421 taagacggta acccctgaat ggcaaacggt agagaaaaaa gaacaaaagg gaacagtcac
      481 tatccgagaa gaaaaaggtg tccgctacaa ccaactatcc tcaactgctc aaaatgataa
      541 cgcaggaaaa ccagccctgt ttgaaaagaa gggcttgacc gttgatgcca atggaaatgc
      601 aactgttgat ttaaccttca aagatgattc tgaaaagggc aaatcacgct ttggtgtctt
      661 cctgaaattt aaagatacca ataataatgt ttttgtcggt tatgacaagg atggctggtt
      721 ctgggagtat aaatctccaa caactagcac ttggtataga ggtagtcgtg ttgctgctcc
      781 tgaaacagga tcaacaaacc gtctctctat cactctcaag tcagacggtc agctaaatgc
      841 cagcaataac gatgtcaatc tctttgacac agtgactcta ccagctgcgg tcaatgacca
      901 tcttaaaaat gagaagaaga tccttctcaa ggcgggctct tatggcaatg accgtacggt
      961 tgtcagcgtt aaaacagaca atcaagaagg cgtaaaagcg gatgatactc ctgcccagaa
     1021 agaaacaggt ccggttgttg acgatagcaa ggtgacttat gatacgatcc agtctaaggt
     1081 acttaaggca gtaattgacc aagccttccc tcgtgtcaag gaatacagct tgaacgggca
     1141 tactttgcca ggacaggtgc aacagttcaa ccaagtcttt atcaataacc accgaatcac
     1201 ccctgaagtc acttataaga aaatcaatga gacaacagca gagtacttga tgaagcttcg
     1261 cgatgatgct cacttaatca atgcggaaat gacagtacgc ctgcaagttg tggacaatca
     1321 attgcacttt gatgtgacca agattgtcaa ccacaatcaa gtcactccag gtcaaaagat
     1381 tgatgacgaa agaaaactac tttcttctat tagtttcctc ggcaatgctt tagtctctgt
     1441 ttctagtgat caaactggtg ctaagtttga tggggcaacc atgtcaaaca atacgcatgt
     1501 cagcggagat gatcatatcg atgtaaccaa tccaatgaaa gacctagcca agggttacat
     1561 gtatggattt gtttctacag ataagcttgc tgctggtgtt tggagtaact ctcaaaacag
     1621 ctatggtggt ggttcgaatg actggactcg tttgacagct tataaagaaa cagtcggaaa
     1681 tgccaactat gtaggaatcc acagctctga atggcaatgg gaaaaagctt ataagggcat
     1741 tgttttccca gaatacacga aggaacttcc aagggctaag gttgttatca ctgaagatgc
     1801 caatgcagac aagaaagtcg attggcagga tggtgccatt gcttatcgta gcattatgaa
     1861 caaccctcaa ggttgggaaa aagttaagga tatcacagct taccgtatcg cgatgaactt
     1921 tggttctcaa gcacaaaacc cattccttat gaccttggat ggtatcaaga aaatcaatct
     1981 ccacacagat ggtcttgggc aaggtgttct ccttaaagga tatggtagcg aaggccatga
     2041 ctctggtcac ttgaactatg ctgatattgg taagcgtatc ggtggtgtcg aagacttcaa
     2101 gaccctaatt gagaaggcta agaaatatgg agctcatcta ggtatccacg ttaacgcttc
     2161 agaaacttat cctgagtcta aatacttcaa tgaaaaaatt ctccgtaaga atccagatgg
     2221 aagttatagc tatggttgga actggctaga tcaaggtatc aacattgatg ctgcctatga
     2281 cctagctcat ggtcgtttgg cacgttggga agatttgaag aaaaaacttg gtgacggtct
     2341 cgactttatc tatgtggacg tttggggtaa tggtcaatca ggtgataacg gtgcctgggc
     2401 tacccacgtt cttgctaaag aaattaacaa acaaggctgg cgctttgcga tcgagtgggg
     2461 ccatggtggt gagtacgact ctaccttcca tcactgggca gctgacttga cctacggtgg
     2521 ctacaccaat aaaggtatca acagtgccat cactcgcttt atccgtaacc accaaaaaga
     2581 tgcttgggtt ggggactaca gaagttatgg tggtgcagcc aactatccac tgctaggtgg
     2641 ctacagcatg aaagactttg aaggctggca aggaagaagt gactacaatg gctacgtgac
     2701 taacctcttt gctcatgacg tcatgactaa gtacttccaa cacttcactg taagtaaatg
     2761 ggaaaatggt acaccggtga ctatgaccga taacggtagc acctataaat ggactccaga
     2821 aatgcgagtg gaattggtag atactgacaa taataaagta gttgtaactc gtaagtcaaa
     2881 tgatgtcaat agcccacaat atcgcgaacg tacagtaacg ctcaacggac gtgtgatcca
     2941 agatggttca gcttacttga ctccttggaa ctgggatgca aatggtaaga aactttctac
     3001 tgataaggaa aagatgtact acttcaatac gcaggccggt gcaacaactt ggacccttcc
     3061 aagcgattgg gcaaagagca aggtttacct ttacaagcta actgaccaag gtaagacaga
     3121 agagcaagaa ctaactgtaa aagatggtaa aattacccta gatcttctag caaatcaacc
     3181 atacgttctc tatcgttcaa aacaaaccaa tcctgaaatg tcatggagtg aaggcatgca
     3241 catctatgac caaggattta acagtggtac cttgaaacat tggaccattt caggcgatgc
     3301 ttctaaggca gaaattgtca agtctcaagg ggcaaacgat atgcttcgta ttcaaggaaa
     3361 caaagaaaaa gttagtctca ctcagaaatt aactggcttg aaaccaaata ccaagtatgc
     3421 cgtttatgtc ggtgtcgata accgtagtaa tgccaaggca agtatcactg tgaatactgg
     3481 tgaaaaagaa gtgactactt ataccaataa gtctctcgcc ctcaactatg taaaagccta
     3541 cgcccacaat acacgtcgtg acaatgctac agttgacgat acaagttact tccaaaacat
     3601 gtacgccttc tttacaactg gatcggacgt ctcaaatgtt actctgacat tgagtcgtga
     3661 agctggtgat caagcaactt actttgatga aattcgtacc tttgaaaaca attcaagcat
     3721 gtacggagac aagcatgata caggtaaagg caccttcaag caagactttg aaaatgttgc
     3781 tcagggtatc ttcccatttg tagtgggtgg tgttgaaggt gttgaagata accgcactca
     3841 cttgtctgaa aaacacgatc catatacaca acgtggttgg aatggtaaga aagtcgatga
     3901 tgttatcgaa ggaaattggt cactcaagac aaatggacta gtgagccgtc gtaacttggt
     3961 ttaccaaact attccgcaaa acttccgttt tgaagcaggt aagacctacc gtgtaacctt
     4021 tgaatacgaa gcaggttcag acaataccta tgcttttgta gtcggtaagg gagaattcca
     4081 gtcaggtcgt cgtggcactc aagcaagcaa cttggaaatg catgaattgc caaatacttg
     4141 gacagattct aagaaagcca agaaggcaac attccttgtg acaggtgcag aaacagggga
     4201 tacttgggta ggtatctact caactggaaa tgcaagtaat actcgtggtg attctggtgg
     4261 aaatgccaac ttccgtggtt ataacgactt catgatggat aatcttcaaa tcgaagaaat
     4321 taccctaaca ggtaagatgt tgacagaaaa tgctctgaag aactacttgc caacggttgc
     4381 catgactaac tacaccaaag agtctatgga tgctttgaaa gaggcggtct ttaacctcag
     4441 tcaggccgat gatgatatca gtgtggaaga agcgcgtgca gagattgcca agattgaagc
     4501 cttgaagaat gctttggttc agaagaaaac ggctttggta gcagaagact ttgcaagtct
     4561 tacagctcct gctcaggctc aagaaggtct tgcaaatgcc tttgatggaa acttatctag
     4621 tttatggcat acatcatggg gcggaggaga tgtaggcaag cctgcaacca tggtcttgaa
     4681 agaaccaact gaaatcactg gacttcgtta tgttccacgt ggatcaggtt caaatggtaa
     4741 cttgcgtgat gtgaaacttg ttgtgacaga tgagtctggc aaggagcata cctttactgc
     4801 aactgattgg ccagataaca ataagccaaa agacattgat tttggtaaga caattaaggc
     4861 taagaaaatt gtccttacag gtactaagac ttacggagat ggtggcgata aataccaatc
     4921 tgcagcggaa ctcatcttta ctcgtccaca ggtagcagaa acacctcttg acttgtcagg
     4981 ctatgaagcg gctttggcta aggctcagaa attaacagac aaagacaatc aagaggaagt
     5041 agctagcgtt caggcaagca tgaaatatgc gacggataac catctcttga cggaaagaat
     5101 ggtggaatac tttgcagatt atctcaacca attaaaagat tctgctacga aaccagatgc
     5161 ttcaactgta gagaaacctg agtttaaact tagctcactt gcttcagatg aagataaggc
     5221 accggcaccg gaactaaaac aagaagtgac gaagcgagaa acagctgaac aaagcttgcc
     5281 agcaacaggt gagagtcaat ctgacacagc cctcttccta gcaggagtta gcctagccct
     5341 atctgctctc tttgtagcaa aaacaaagaa agactagtat ttagtaaaac ctcttaacaa
     5401 gattacgaaa tccgtttcct gtctttccca atgaggttta aagtacagaa aaagcctgag
     5461 aagacatctt ctcaggcttt tgttaagcat ataaatgcaa tagtgctatg aaaaaatcac
     5521 ccagaaaaat ctgggtgata aatgttatgg ttgtgctggt tgaggattct gattttggtt
     5581 ctggttttgt tgaccaggcg ttgtatttgg ttgctggtta ttggtattat tgtttgcact
     5641 attattcgtg cttggagtag ttgagcttga ctgtgaagtt gaactttctg atgtcgagct
     5701 cgaactttct ggtgttgggg cctgttgcgg agcaggagca gtccatgcag aacgagcacc
     5761 ttttttaaag acgaactcac cacttcgata gagaccttct ggcatagtcc agtcgccagg
     5821 gttgttatcc tcagacagat aagtcatcat tgaacggtaa accttagctg caacatagaa
     5881 gccatcacca acgataggag tcaggcggtt tgagtaacct gtccataccg ccattgaata
     5941 tttgcgagta taaccaacaa acatttcgtc tggagctaca taaccagtat ttttgatgta
     6001 gttttcaatt tcatcatctg tatagtttga tgtaccagtc ttaccagctt gaggtagcca
     6061 agggagataa gcaccacgac ccgttccgta tgccaagaca gtcttcatca tttctgtcat
     6121 catataggcg gtcgtctctt tcatggcacg agtaccagga tcagcgtatt cttttgagct
     6181 accatcgcta aagacgattt tgttgatata cattggttta tggtagatac caccgttagc
     6241 aaaagcagcg taagcagcag ccattttctc actacttgct ccgtactttt tgtttgactc
     6301 agtcgtatta cttgaaatgg cgtttgcata atgcatgctt ggatagtcga taccaagacc
     6361 attaagaaag gttttagctc tatctagacc gaccttattc aaagtctcaa cggctgtgac
     6421 attacgtgac tgttgaagtg catactggat tgtaatattt ccaaagtaga ctttatccca
     6481 gttgtagagg ggagtatcag tacctggata gttataagga acatcatgta caatcgaagc
     6541 agttgagtcg tagatgtcat actctaaggc aggagcatag tctgtgattg gcttcatggc
     6601 agaaccccag tcacggttgg tttcaacagc ttggttgatt ccgaaggaaa cattgcttga
     6661 ttggtgacga gaacctaact gagcaataac tttcccgtta gaaacatcaa caatggtaga
     6721 agcgacttgc aattcatcgt ctggataggc aacgtattcg tctgtattgt aaatatccca
     6781 cagatgtttt tgagcttctt ggtctacatt tgtgtagaca tccatcccag ttgtgagcag
     6841 gttatagcct gtttcttctt caacttgatt gatgacttcc ttgaggtaat tatccatgta
     6901 agcagggtaa ttacttgctg atttgagact ttgtagtcca tcagtaattg gtgtattgac
     6961 tgctttctca tactgttcag cagagatgta gccttgattt ttcatttcag ataagaccaa
     7021 gtttcggcgg tcttgggctg cttctggatg tgaatagggg tcatattggt ttggtgcctg
     7081 aggcattcca gccagcaagg ctaactgagg taaacttaaa ttattgaggt ctttaccata
     7141 gtagttttga gctgctgtct gcattccata gttcccatta gacatgtaga ccttatttat
     7201 atagtaggtc aagatttctt gcttggttgc tttttgttct aactgaatcg ctaaccaagc
     7261 ttcctgagcc ttacgagaaa tagtctggtc ggaagtcgaa gttgaaaagt aagtcaactt
     7321 aatcaactgt tgagtgagag ttgatccacc ttggagggaa ttgctttgca gattgcgcaa
     7381 gaaagctccc aggatacgga tggtatcaat ccccctgtgg tcgaagaagc gatggtcttc
     7441 gatagaaacg attgccttaa ccaaatctgt gggaatatca ttagcttggg cattgacgcg
     7501 gcgttcagaa cccaagtcag caatgagttg atttttattg tcgtagattt tactagaagt
     7561 tgttgcaact agtttactct cggataggct aggagccttg ctaacatagt agaaaaaaac
     7621 tcctccgcct aagacaatgg ctgcgataac caagcttaag aagctaatgc tcagatactt
     7681 gattaggcgc agaatcgttg gtttgttcat cttgttttac cacctaataa atgttctttg
     7741 ataacattga gataaggaat ttgagggaag gcaccagcct tgatttcata tccatattct
     7801 cgaatatatt caagtggcat tgatttttgt cccttatctt gatgatagaa gcgaatcaaa
     7861 tcgaatgccg gcaataagta ggtttcttgc tgagaagaaa agtgaagaag gacaaagcag
     7921 attccttgtt gggcaaggac ttgttccata tgctgaatct gatgtggatg aaaatttttc
     7981 atcggaatcg cacgtttttg ttttgtttcc ttgacttcaa agtcgatgta atatccatta
     8041 taaacgccag aatagtccgt cgttgaagct tgtcgaaaat aggcttcaac aatcttggca
     8101 cgacttcgtt gtggatagtc cacttgtacg atttgaatag gagttggttt cttatgtata
     8161 acagccaagc cttgagacaa atagtagtcg ttggtagcat tgatcatctt ttcaaaagac
     8221 attcctcgat ttgcgaaatt tttgggttga gaaagagatg tttgtctttt ttgtgatgaa
     8281 actttatgtg gatagttgac cataattctc cttattggta caataacatc actttattat
     8341 accataattt gcacagaaag ggttaaaaat ggctacagct ttggttttag gctattctgc
     8401 ttttgatttg ggtttatttt ctgataagga tcctcgtctt aaattgatta aaaaagcgat
     8461 acgtaaagat ttagaggcta tggcagcaga tggggtgtct tggcttgtgt ttacagggag
     8521 tttggggttt gaatattggg ttttagaggt tgctcaggag atgaaaaccg aatacggttt
     8581 ccagttggcc accatttttg cttttgaaac ccatggggaa aattggaatg aaggcaatca
     8641 gatgaaactg agtcgcttta agcaggtaga ctttgtcaaa tatgcctatc ctcgctatga
     8701 acacaagggg cagttaagag attaccagca gtttttgctg gaaaacacga ctagttccta
     8761 tcttttttat gatgaagaaa atgaaacaaa actagcttat ttttaccaaa agatgaaaaa
     8821 tcaagaggac tattttataa agagattaac atttgatcaa ttaaatgaac ttgctgaaaa
     8881 tttttccgaa aattgaagct ttgaccttga tttttgtttg ccttttttta tataataata
     8941 ctagcaagcg agaatggaga gagacatggc aagtattatt ttttcagcga aagatatttt
     9001 tgaacaagag tttggacgtg aagtccgtgg ctataataaa gtagaagttg acgagttttt
     9061 agacgatgtc atcaaggact atgaaaccta tgctgccttg gtcaagtcac ttcgtcagga
     9121 aattgcggat ttgaaggaag aattaactcg taaaccgaaa ccttcaccag ttcaagcaga
     9181 accccttgaa gcggcaatta caagttctat gacgaatttt gatattttga aacgcctgaa
     9241 tagattggaa aaagaagttt ttggtaaaca aattttagat aactcagatt tttaagtagt
     9301 tatttgagat gtgcaatttt tggataatcg cgtgaggaga attgcttctc atgaggaaag
     9361 tccatgctag cacaggctgt gatgcctgta gtgtttgtgc taggcgaaac cataagccta
     9421 gggacgagaa atcgttacgg cagttgaaat ggctaagtcc ttggataggc cagagtaggc
     9481 ttgaaagtgc cacagtgacg gagtctttct ggaaacagag agagtggaac gcggtaaacc
     9541 cctcaagcta gcaacccaaa ttttggtcgg ggcatggagt acgcggaaac gaacgtagta
     9601 ttctgactgc tatcagctag agctgttagt ggtagacaga tgattatcga aggaagtggt
     9661 cctagtcact tctggaacaa aacatggctt atagaaaatt gcatataggt tggggctgag
     9721 aaattttctc aacctcattt tttaaagtgg acatatagaa aggtcttgca agactgtaac
     9781 atgaaaaaag aatttaattt aattgcaact gtggcagcag ggcttgaggc tgtcgttggt
     9841 cgtgaagtgc gagagttggg ctacgattgt cagattgaaa atggacgtgt tcgttttcaa
     9901 ggagacgtga gagctattat cgaaaccaac ctttggctgc gggcagcaga tcgtatcaaa
     9961 attatcgtag gaacgttccc tgctaagact tttgaagagc tatttcaggg agttttcgct
    10021 ttagattggg aaaattattt accacttgga gctcggttcc cgatttcaaa agctaaatgt
    10081 gttaagtcca aacttcacaa tgagcccagt gttcaggcta tttctaagaa agctgttgtc
    10141 aagaaattgc agaaacacta tgctcgccca gaaggggttc ctctgatgga gaatggccca
    10201 gagtttaaga ttgaggtctc tattctcaaa gatgtggcaa ctgtcatgat tgatacgacc
    10261 gggtctagcc tctttaaacg tggttatcgt accgaaaaag gtggcgctcc tatcaaggaa
    10321 aatatggcag cagccatttt acaactttct aactggtatc cagacaagcc tttgattgat
    10381 ccgacctgtg gttcggggac tttctgtatt gaggcagtta tgattgctag aaagatggcg
    10441 ccaggtcttc gtcgctcttt tgcatttgag gaatggaact ggatcagcga tcgcttgatt
    10501 caagaagtgc gcacagaagc ggctaaaaaa gtagaccgtg agcttgagct ggatatcatg
    10561 ggctgtgata ttgatgctcg catggtggaa attgctaagg ccaatgctca ggtagctggt
    10621 gttgcaggag acattacttt taagcagatg cgcgtgcagg atttacgttc cgataaaatc
    10681 aatggagtaa tcatttccaa tccgccttat ggtgaacgtt tgtcagatga tgcaggggtg
    10741 accaagctct atgctgagat ggggcaagta tttgcaccgc tgaaaacttg gagcaaattt
    10801 atcctgacta gtgatgaagc ttttgaaagc aagtatggta gccaagcaga taagaagcgt
    10861 aagttataca acggaacctt gaaagtggat ctatatcaat attttggtca gcgtgtcaaa
    10921 cggcaagagg taaaatagaa aggatactca tgagtaaaaa aagacgaaat cgtcaaaaaa
    10981 aagaaggtca agaaccgcaa tttgattttg atgaagcaaa agagctaaca gttggtcaag
    11041 ctattcgtaa aaatgaagaa gtggaagcag gagtcttgcc tgaggattcc attttggaca
    11101 agtatgttaa gcaacacaga gatgaaattg aggcggataa gtttgcgact cgtcaataca
    11161 aaaaagagga gttcgttgaa actcagagtc tggatgattt aattcaagag atgcgtgagg
    11221 ctgtagagaa gtcagaagct tcttcggagg aagttccatc ttctgaagac atcttactac
    11281 ccttgcctct ggacgatgag gagcaaggct tggatcctct attgctagat gatgaaaatc
    11341 caacagaaat gactgaagaa gtggaagagg agcaaaacct ttctcgtctg gatcaagagg
    11401 actcagaaaa gaaaagtaaa aaaggcttta ttttgaccgt tttggcgctt gtatcagtaa
    11461 ttatttgtgt cagtgcttat tatgtctacc gtcaagtggc tcgttcgacc aaggaaattg
    11521 aaacttctca atcaactaca gccaatcaat cggatgtgga tgattttaat acactttatg
    11581 acgcctttta cacagatagc aataaaacgg ctttgaaaaa tagccagttt gataaactga
    11641 atcaactcaa gactttactt gataagctgg aaggtagtcg tgaacatacg cttgccaaat
    11701 ctaaatatga tagtctagca acgcaaatca aggctattca agatgtcaat gcacaatttg
    11761 aaaaatcagc tattgtggat ggtgtgttgg ataccaatgc caaagccaaa tcggatgcta
    11821 aatttacaga tattaaaact ggaaatacgg agcttgataa agtgctagat aaggctatca
    11881 gtcttggtaa gagccagcaa acaagtactt ctagctcaag tttaagtcaa actagcagct
    11941 caagttcaag tcaagcaagt tcaaatacga ctagtgagcc aaaaccaagt agttcaaatg
    12001 agactagaag tagtcgcagt gaagtcaata tgggtctctc gagtgcaggg gttgctgttc
    12061 aaagaagtgc cagtcgtgtt gcctataatc agtctgctat tgatgatagt aataactctg
    12121 cctgggattt tgcggatggt gttttggaac aaattctagc aacttcacgt tcacgtggct
    12181 atatcactgg agaccaatat atccttgaac gtgtcaatat cgttaacggc aatggttatt
    12241 acaacctcta taagccagat ggaacctatc tctttaccct taactgtaag acaggctact
    12301 ttgtcggaaa tggcgctggt catgcggatg acttggacta ctaagcagtc gttacaaaat
    12361 tctttctttt caaaagtaaa aatgataaaa taaaacaaat gaaacaagag gagtgtcaaa
    12421 tgacaaaagc taactttggt gtcgtaggta tggccgtaat gggtcgtaac cttgccctta
    12481 atattgaatc acgtggttac acagttgcta tctacaaccg tagtaaagaa aaaacggaag
    12541 atgtgattgc ttgccatcct gaaaagaatt ttgtaccaag ctatgacgtt gaaagttttg
    12601 taaactcaat cgaaaaacct cgtcgtatca tgctgatggt tcaagctgga cctggtacag
    12661 atgctactat ccaagccctt cttccacacc ttgacaaagg tgatatcttg attgacggag
    12721 gaaacacttt ctacaaagat accatccgtc gtaatgaaga attggcaaac tcaggtatca
    12781 actttatcgg tactggagtt tctggtggtg aaaaaggtgc ccttgaaggt ccttctatca
    12841 tgcctggtgg acaaaaagag gcctacgaat tggtttcgga tgttcttgaa gaaatctcag
    12901 ctaaagcacc agaagatggc aagccatgtg tgacttatat cggtcctgat ggagctggtc
    12961 actatgtgaa aatggttcac aacggtatcg agtacggtga tatgcaattg atcgcagaaa
    13021 gttatgactt gatgcaacac ttgctaggtc tttctgcaga ggatatggct gaaatcttta
    13081 ctgagtggaa caagggtgaa ttggatagct acttgatcga aatcacagct gatatcttga
    13141 gccgtaaaga cgatgaaggc caagatggac caatcgtaga ctacatcctt gatgctgcag
    13201 gtaacaaggg aactggtaaa tggactagcc aatcatctct tgaccttggt gtaccattgt
    13261 cactgattac tgagtcagtg tttgcacgct acatttcaac ttacaaagaa gaacgtgtat
    13321 atgctagcaa ggtgcttcca aaaccagctg ccttcaactt tgaaggagac aaggctgaat
    13381 tgattgaaaa gatccgtcaa gccctttact tctcaaaaat catttcatac gcacaaggat
    13441 ttgctcaatt gcgtgtagcc tctaaagaaa acaactggaa cttgccattt gcagatatcg
    13501 catctatctg gcgtgatggc tgtatcatcc gttctcgttt cttgcaaaag attacagatg
    13561 cttacaaccg cgatgcagat cttgccaacc ttcttttgga cgagtacttc ttggatgtta
    13621 ctgctaagta ccaacaagca gtacgtgata tcgtagctct tgcggttcaa gcaggtgtgc
    13681 cagtgccaac tttctcagca gctattactt actttgatag ctaccgttca gctgaccttc
    13741 cagctaactt gatccaagca caacgtgact actttggtgc tcacacttac caacgtaaag
    13801 acaaagaagg aaccttccac tactcttggt atgacgaaaa ataagtaggt cagccatggg
    13861 gaaacggatt ttattacttg agaaagaacg aaatctagct cattttttaa gtttggaact
    13921 ccagaaagag cagtatcggg ttgatctggt agaggagggg caaaaagccc tctccatggc
    13981 tcttcagaca gactatgatt tgattttatt gaacgttaat ctgggagata tgatggctca
    14041 ggattttgca gaaaaattga gccgaactaa acctgcctca gtcatcatga ttttagatca
    14101 ttgggaagac ttgcaagaag agctggaagt tgttcagcgt tttgcagttt catacatcta
    14161 taagccagtc cttatcgaaa atctggtagc gcgtatttcg gcgatcttcc gaggtcggga
    14221 cttcattgat caacactgca gtctgatgaa agttccaagg acctaccgca atcttaggat
    14281 agatgttgaa catcacacgg tttatcgtgg tgaagagatg attgctctga cacgccgtga
    14341 gtatgacctt ttggcgacac ttatgggaag caagaaagta ttgactcgtg agcaattgtt
    14401 ggaaagtgtt tggaagtatg aaagtgcgac cgagacaaat atcgtagatg tctatatccg
    14461 ctatctacgg agcaagcttg atgttaaagg acaaaaaagc tacattaaaa ctgtgcgtgg
    14521 tgttggatat accatgcaag aatagaaaag cagttgcagt tgtgtaactg cttttttgag
    14581 gagtttttat atattgacat gcagtcgggt ctttgctaca atcgttttag gaggatatat
    14641 agatgaaact tttgaaaaaa atgatgcaag ttctactagc agtctttttc tttggtttgc
    14701 tagctacaaa tacggtattt gcgaatacca caggtggccg atttgttgat aaggataata
    14761 gaaaatatta tgtaaaagat gatcataaag caatctattg gcataaaata gacggtaaaa
    14821 cttactattt tggtgatatt ggagagatgg ttgtcggttg gcaatactta gaaattcctg
    14881 gaacaggtta tcgtgataat ttattcgata accaaccagt taatgaaatt ggccttcagg
    14941 agaagtggta ctattttgga caagatggtg ctttgctaga acaaacagat aaacaagtac
    15001 tagaggcaaa aacgtctgaa aatacaggaa aagtatacgg tgaacaatat cctctatctg
    15061 ctgaaaagag aacttattat tttgataata attatgctgt aaagacaggc tggatttatg
    15121 aagacggcaa ttggtattat ttaaataagc taggaaattt tggcgatgat tcttacaatc
    15181 cactaccaat tggtgaagtt gctaagggtt ggactcaaga ttttcatgtt actattgaca
    15241 ttgatagaag caaacctgct ccatggtact acctagatgc ttcaggtaag atgcttacag
    15301 attggcaaaa agtaaacgga aaatggtatt attttggctc ctctggttct atggcaacag
    15361 gttggaaata tgtacgaggc aaatggtatt acttagataa taaaaatggt gatatgaaaa
    15421 caggatggca ataccttggt aacaagtggt actacctccg ttcatcagga gcaatggcaa
    15481 ctggttggta tcaagatggt tcaacttggt actacctaaa tgcaagtaat ggaggtatga
    15541 agacaggttg gttccaggtc aatggcaaat ggtattatgc ctatggttca ggtgctttgg
    15601 cagtgaatac gaccgtagac ggctattctg tcaattataa tggcgactgg gttcggtaat
    15661 gacagggtgc ttataattat attagaacac tcgcttaatc aaagaagtga ctttgttgta
    15721 cttgacctga cgattgggga gattaaaaag catgtgaaat ctacacatgc ttttttgtta
    15781 gttatgagtg ttgaacttga ctaaccattg acaaaagata agagcaatca aaataagata
    15841 caaaaatatc aatgctaact gcaccgaaac gcccgatgct atggataggg ctaataggga
    15901 gagcagggac ggggcgagaa tgtccactgt gctcagagca gagcccaccg tccccatctt
    15961 gtcactgggg atataggcaa agacagctgc ttgtaggcga gggctgagaa tgcctataat
    16021 cgtagaactg atgaaactgg tcatgaaaat tacgatatag gcttgataaa ggatactagt
    16081 tattagagcc aatagggaaa tctcacagaa aaccaagagg tgcgtgaaac ggatattttt
    16141 aaatagatta ctgctggtca tattgccaag gatagctcca caagacatga tgatggagag
    16201 cagggcgagg agttgcccaa tgcttagttg tgctagtgtt tgttggatga agcggagggc
    16261 aatcagccga ggggacacat ccaagatggc aatcatactg gtcgtcagaa acagtagtat
    16321 gacggtatct tttaacctca ggagcacagc cagcgattgt ttaagatgtt gaaaatattt
    16381 cttaaaactc agtgctgtat gtgacatttc aatccttttt tcaacctcat acaaggtatg
    16441 tctaataaca tacaggccca aaaaggcaat gacaaaagtc agagtgttga taagggaaat
    16501 agtttgaata cttataacat tgataaggaa tgcgccagcc agattggcga caatgcggac
    16561 aaccctcatc agagactgcc taaaccccat ggcatcatgc aggtcgtcct taattacact
    16621 gatgtagagg gcgttcatca tgtaggcaga caggtagctc aaggtgtcag agatgaggtt
    16681 gatgatgaca atcataatga ctgaaactaa tgaaaagggc gacaagacga gaaatactgt
    16741 caaaatagcg tagagcagga ttttgatttt ggcaatccag agtgcgtgtt tgactctatt
    16801 ttcttgaaaa tctcctagaa tcccgagaaa gagttgcgat agtaggggaa ccatctctga
    16861 aatggaaatc agtaaaatcg ctagcggagc gaaattgtac tgagccacat aattgataat
    16921 ggcgaggtag aaaatactgt cgccgaatga tgaaaaacag ctgtttagca gcaactgtct
    16981 atatatgttg tttgtccaaa ataatttcat acttttctcc ttatgtctgc acatcgaatg
    17041 ggaatttatt atttaataga caccaatata ccccatgctt cctattccga agtcaaacaa
    17101 atctgcttta ccactatctc cttgaaacat cagtaaaccg tgatcatctc gttcagaaca
    17161 tgctaaaatt acctgttgga tttttttcat cagtaatttg ttttgattgt agacagtggt
    17221 ttgtagtaag ctagttattc cgtggcaata tcctgtcatc atttttagat gatggttaaa
    17281 aacaaattct tgatatttac taataatatc tttgtttccg tcacaatcgt acatacaaag
    17341 atataagatt attccggaaa ttcctttgca ccaagaaagt tgtaaatttc ctaaattttc
    17401 agtattagaa gtaacttttt ctaataattt ttttaattca gtatggaata tatggagatc
    17461 attatagaac atagaaggtt ctaagacttt agaataggca aataaagcat atgcaatttc
    17521 acaatatcca tgagcaaagc tatagtctaa aatgctttct ttggctgtat ctctttgtag
    17581 gaaataatga taaatttccc catatactat tgtggatgaa agttttaaga gtattgtcat
    17641 tggtaaattg atagtatttt attaatggta ataggacacc agattttccc aatgcaaagt
    17701 ctatttcctc tagagtatca taattctcta ttaatttctt agcggtgacg tttgctaatt
    17761 gtagatagta ttgattttta ctgatagaaa ataaatgtag gagtaaccat agatagcctg
    17821 catcaccaaa tagcagtgag tattgatatt catatgtgtg tgtcgttcta attgtcactg
    17881 ccttcttgat gatagagagt attttagaat ccagttctgg ggggtacaat ttatttagag
    17941 gaaacagtag tccagctatt ccgtgttgga atgataaatt acttacgaaa tttccaaatt
    18001 ctgttgaaga agtgatcctt ccttcagaat ttacagtttt atctagtaaa aatcgtagaa
//
LOCUS       DAVMGZ010000028        17979 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000028 DAVMGZ010000000
VERSION     DAVMGZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17979
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.029"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..120)
                     /locus_tag="VYQ93_000799"
                     /pseudo
     CDS             complement(1..120)
                     /locus_tag="VYQ93_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
     gene            complement(257..403)
                     /locus_tag="VYQ93_000800"
     CDS             complement(257..403)
                     /locus_tag="VYQ93_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335521.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
     gene            complement(400..855)
                     /locus_tag="VYQ93_000801"
     CDS             complement(400..855)
                     /locus_tag="VYQ93_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335522.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTVYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            complement(992..1876)
                     /locus_tag="VYQ93_000802"
     CDS             complement(992..1876)
                     /locus_tag="VYQ93_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV3335523.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(1880..2266)
                     /locus_tag="VYQ93_000803"
     CDS             complement(1880..2266)
                     /locus_tag="VYQ93_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV3335524.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(2259..3602)
                     /locus_tag="VYQ93_000804"
     CDS             complement(2259..3602)
                     /locus_tag="VYQ93_000804"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV3335525.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRFFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(3684..4568)
                     /locus_tag="VYQ93_000805"
     CDS             complement(3684..4568)
                     /locus_tag="VYQ93_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3335526.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(4580..5197)
                     /gene="cysE"
                     /locus_tag="VYQ93_000806"
     CDS             complement(4580..5197)
                     /gene="cysE"
                     /locus_tag="VYQ93_000806"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV3335527.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(5213..7426)
                     /gene="pnp"
                     /locus_tag="VYQ93_000807"
     CDS             complement(5213..7426)
                     /gene="pnp"
                     /locus_tag="VYQ93_000807"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV3335528.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDIIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETSKDSE"
     gene            complement(8111..8977)
                     /gene="metF"
                     /locus_tag="VYQ93_000808"
     CDS             complement(8111..8977)
                     /gene="metF"
                     /locus_tag="VYQ93_000808"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV3335529.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQYMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     NAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(9041..11290)
                     /gene="metE"
                     /locus_tag="VYQ93_000809"
     CDS             complement(9041..11290)
                     /gene="metE"
                     /locus_tag="VYQ93_000809"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV3335530.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSFVAAYQEVFAKLADLGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLFQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGKNAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(11551..11817)
                     /locus_tag="VYQ93_000810"
     CDS             complement(11551..11817)
                     /locus_tag="VYQ93_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3335531.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            complement(11943..12390)
                     /locus_tag="VYQ93_000811"
                     /pseudo
     CDS             complement(11943..12390)
                     /locus_tag="VYQ93_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            12546..13547
                     /locus_tag="VYQ93_000812"
     CDS             12546..13547
                     /locus_tag="VYQ93_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV3335532.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FLLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVRDLDIVSFQNSDHRCIFTEITF"
     gene            complement(13615..16017)
                     /locus_tag="VYQ93_000813"
     CDS             complement(13615..16017)
                     /locus_tag="VYQ93_000813"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV3335533.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAEDALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(16094..16603)
                     /locus_tag="VYQ93_000814"
     CDS             complement(16094..16603)
                     /locus_tag="VYQ93_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3335534.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(16603..17649)
                     /gene="pheS"
                     /locus_tag="VYQ93_000815"
     CDS             complement(16603..17649)
                     /gene="pheS"
                     /locus_tag="VYQ93_000815"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV3335535.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
BASE COUNT         5029 a         4070 c         3480 g         5400 t
ORIGIN      
        1 taacttaagc aatccccata atcgtctcga tttcttcttc cattgcttcc agataatcac
       61 tcgtaggcga gtacgcaagc gctcatctat gctagtgact atacttttca tatttataat
      121 tcattccttt cgtttcactc aaggcacaac acagaatgaa aaagtgttgt gatctttatt
      181 ttgttttata ataatagtga gaaagcctat cactactaca aatcacgggg aggtgaataa
      241 gtgagtggta cagccactac ctcgcatatt ttgtcacatc atttaacggt acataataag
      301 ttgtaccatc tgaataagtt gctacaatat catttgcatg ctctccttca cctttagcaa
      361 aggttggagc tcctgctgga tgatttttat ttgcctcttt caatttttca ataatggcat
      421 tttttctgta tcttttatat tatcaggatt tttcactaag attttgtctg gatatgtcgg
      481 tttagcagaa acaattttta ctgttacttc ttttttattc gaagcacttg tccagtttcc
      541 agcattatct ttagcattta attttacagt aattcctgaa ctaggaactt cagtagcagg
      601 ttgattatca acattattca actttaattt caaaagagct gttgcatcag acgttttatc
      661 aatcgttata tataatgatg aattgttatt ataaacagtt ccttcatatt tagctgtttg
      721 tgagctactt gaaacagaac tgaaattata cccactacct ccctgattat cttcaatgct
      781 tacgtctaaa tgaacttccc cactattatt tggcttagca acaactgtta tagtaaaata
      841 acataaaatt tgcataaata gattagggaa atcaaagcag cttctaggaa tgttttagca
      901 gtcacagtgt actttcccag catcaagcca ctataactct gcacataaaa atggagaaga
      961 tggcaatcct cttctccaaa tattaacttc tttacaaacc aactatagtt gacaaagaac
     1021 ctaaaatcaa ttgataacac aaggtcaggt cggtcaactc tttcaactga agccctgtca
     1081 actcttccca tttatcaatc ttgtattgga gagaattgcg gtgcagatag agttgctggg
     1141 ctgttttagt gagaacagca ctattttccc aaagagagag aatgatttcc tgaatctgat
     1201 cttgatccaa aatcatctgg tgtagacatt ccttgattgg cttcaagtcc acgagtcttt
     1261 ctcccatact ccaaagatag agctgagaaa aagtatgaac accttggtga ccctgacgcc
     1321 accatgtctt gaacaaatcc cgctcagctt tgattaagtc tgatagggct tgatgtcccg
     1381 tctgagacca aacctgaccc aacatgatag aaagacgaag tccaaagtca tactcaaccg
     1441 cttcaatcgt atcacttaaa atatctctta cagaagtgta tttgtcttgt tgaagcacga
     1501 aaacataatc ctgagctccg acctgtagca ctgtctgaca attcggaaaa agagtccgca
     1561 tcatatctag ccaagaagcc agattttcct gctgaaaata agaaagatgg caataaacca
     1621 actgaatctt tttaaaaact tgcggtgcct gtcccttgcc ctcaaccaga taggaatacc
     1681 aagggtttag cgaacgagcc tgctcctgct gggtcaaaag ggcaaccaac tgcttttcac
     1741 gctcgctgag cccagcttcc tccagcaaaa tccactgctg agaagctaaa gggagcgtga
     1801 gatagccctc tttctctact ggttggtctg aaatccgagc ctcaggaaac cagtcttgta
     1861 gttcttttgc cctcatgttc tagccctcca ctttttggat gcaccatgaa accaaactct
     1921 caagacgttc cagattctca gtcatatgga gatagcccat aaccgcttca aatcccgtgg
     1981 acatacgata agtcacgaca tctgcatttt tagcctttgt gtggctattg gtattgcggc
     2041 cacgtttgta gatttcttct tctttttccg ttaggacctg ctcctccaac atgagagcaa
     2101 tcaggcgagc ctgagccttg gctgacacgt acttagttgc ttcttgatgg agtttattgg
     2161 gtttggtcat acctttgagg atgaggtgac ggcgaatata catagaatac accgcatccc
     2221 cctcaaaggc tagcgcaatc ccgttaatga gattgacatc aatcacgtgt ccacctcact
     2281 ccatccttgg tatcaaggag cttaattcct tgagtaacca attggtcacg gatttggtct
     2341 gctgtcgcaa agtcacgatt ggcacgcgcc tcttggcgtt tttgaatcaa gtcttcaatc
     2401 tctgcatcca aaacttcctc aacaaagaca attccaaaaa tttctaacat atctgcaaga
     2461 gcttgcttga cacttgcatc atagttccct gagttgatcc atttggccat ttcaaagaca
     2521 actgtgatac cgttggcagc attaaaatct tcatccatag ctgctacaaa cttatcttta
     2581 aagttttgta actcttgggc atccacattt cctgtaaatg gttgttcgta agtattcttg
     2641 agatacttga gattggtctc ggcatcgcga actgcctttt ccgtgaagtt gataggctta
     2701 cggtagtgct gggtcgcaaa gaagaaacga agtacttgcc catcaagagt tttaagggca
     2761 tcgtgtaccg taatgaagtt acccaaggac ttagacattt tgacattgtc gatattgaca
     2821 aagccattgt gcatccagta gttagcaaaa gccttgcctg ttttagcttc agactgggca
     2881 atttcattgg tgtggtgtgg aaactctagg tcagctccac caccgtggat atcaatggta
     2941 tcacctaaaa tctctgtcga catgactgaa cactcaatat gccaacccgg acgtccaggt
     3001 ccccaaggac tatcccaaga aatctcacct ggtttggaag atttccatag agcaaagtct
     3061 acaggatttt ccttacgagc cgtttcttca tcggtacgac ctgaagcacc tagctccaaa
     3121 tcttccaagg ttttattagc caatttagca tagttgtggg atttttctac acggaaatag
     3181 acatcccctt gactctcata ggcaaagcct ttctcgatca agtcttccac aaaacggatg
     3241 atgtctgcca taaactccac tacacgcgga tggcgagtcg caggtttcac gcccaatgcc
     3301 gtcacatcct cacgaaaggc agcgatgtac ttatccgcaa cctcctgagg cgtgatacct
     3361 tcttccctgg cacggttgat aatcttatca tccacatctg taaaattgga aatataggca
     3421 accttatacc cacggtactc aaaaaagcga cgaatcgtat caaaagctac cgtcgaacgg
     3481 gcgtttccta cgtggatata gttgtacacc gttggcccac aaacatacat cttgatcttg
     3541 ccgtcctcaa tcgggacaaa ttctcgcaaa tcacgagaca tggtgtcata gattttaatc
     3601 ataaatcata atcaggaaag ctgaaatcca agaacaatta gtttcatcac taaaagttca
     3661 agtaaatttc agtccgaata tctctacact tcggaatccc ttgctccttt ctcattcaga
     3721 taaaccacct gagtctgttt gacaaagcca attttttcat acaaacgttt ggcacctaca
     3781 ttgctatctt ccactgcaat ctgaaattcc ttgtcatttt gctcaattag ttggttgacg
     3841 agggattttg ctaagtagct tccatagcct tttccacgtt caggttccaa tattgctaaa
     3901 ccgtagaggt aattcgtatt agtcgataaa tcaaccgtac aagttccaat aacctgacca
     3961 gcttttaata aaatatatag tcggctttct ggatctttca gagcttcagc gacatatcta
     4021 tccacaactt ctctcgattc atgttcctct gaaaatgcct gaaattttaa ttgactaatt
     4081 tgatcctgat acgaactatc tgctaacaaa acttcaagat gggaaacatt tgctaacgga
     4141 taaggtcttc tatccttacc taaccaagtt tctgtctctt catcctcgat tagtccccag
     4201 ttactggcaa agtcaggatg attctctaaa aaaatacgtt ctgtctgaaa agtgactgac
     4261 cgaatgggga aagaagctgt ttctctctca aaactagtaa acaatgcacg cgcaatcccc
     4321 tgacggcgat gacctggatg aaccagtatc gtcacttcta catcttggtc atctgcatag
     4381 acagttaata aaccaacaag ttcgcctttt tcataataaa ggaaaaaggc gggcatgttt
     4441 gggtcaaaat taagcatgtt agagagatag ggatcgcgat aggtaccgtc atagttttgg
     4501 caacagttaa ttactttttt cgcctcagat agctcctctt ggcttaactt gtttcttgct
     4561 tgaatcatat aggtatcctc tacaaaccag acgatctgtg actggcatct ttagcctgct
     4621 cgagtttatt gacataatac tctcgttttt cttcgacttc gtgaatgaca ggctcatctt
     4681 tcttaccatg aagacggaca atcttggccg gaataccgac aaccgtcacg tcactaggta
     4741 catctgctac gacaactgct gcagcaccga ccttggcatt ttcaccaatt tccacaggcc
     4801 cgataacttg ggcatgggct gatatgaggg ctccctttcg tacagtcgga tggcgtttgc
     4861 cacagtcttt ccctgttccc ccgagagtca ctccgtgata gagaagaacg cctttttcaa
     4921 caatcgctgt ctctccaatc accagaccag aaccatggtc aataaaaaca cctgaatcaa
     4981 tctgggctcc tggatgaatc tcaatctgag tccaaaagcg ccaaaactga ctgtacatac
     5041 gagctaatag tttgaagccg tgcttccaga gaaaatgcga gagacggtgg gccgccaagg
     5101 ccttgacacc tggataagtc agcaaaacct ccaaagtggt gcgggccgct ggatcatttt
     5161 cttttacaat atcaatggtt tcgcgccacc accccataca tttctccttt tcttattctg
     5221 aatcttttga tgtttctgta aattctttct taggtttgta atccttttga tgacgtgggc
     5281 ggtgagggcg ctcagacttt tcaccttttt catcatgctc aggttttggc ggacgaggta
     5341 gaagagcctt catagaggca tcgatacggc ctttttcatc aattttgata accttaacat
     5401 caacttcatc cccgatttct accaaatcct ctacacgatt ggtacgagtc caagccatct
     5461 cagagatatg aacaagggca tctgtcttat caaagaggtt aacaaaggca ccaaatttct
     5521 cgatacgaac gactttagca cggtaaactt catccacttt ggcttcacga accaaaccag
     5581 caataatttc tttggcacgg ttaatagcat cttggtcact agagtagata gacacatttc
     5641 cttcttcgtc tatatcaatc ttaacacctg tttcagcgat aatcttgtcg atggtttctc
     5701 cacccttacc gatgacaatc ttaatcttgt ccacatcaat cttgatcgta tcaattttcg
     5761 gagcagttgg agccaattct ggacgaactt ctggaatggt tgcttcaatg atatcaagga
     5821 tttcaaaacg cgctttcttg gcttgagcaa gagcctccgt caagatttct gcagtaatcc
     5881 cttgaatctt gatatccatt tgaagggctg taatcccatc acgagtacct gcaaccttga
     5941 agtccatatc tccaaagtga tcttccaaac cttggatatc tgtcaatact gtgtagttat
     6001 ttccatctga gataagtccc atagcaatac cagctactgg cgccttgatt ggcacaccac
     6061 cagccataag ggcaagagtt cccgcacaga tagaagcttg agatgaagaa ccgtttgatt
     6121 ccaaaacttc tgctactaga cggatagcgt atgggaattc ttccaagctt ggcaagactt
     6181 gagcaagagc acgctcacca agggcaccgt gaccgatttc acgacgacct ggcgcaccgt
     6241 aacgacctgt ttcccctaca gaatattgag ggaagttata gtggtgcata aagcgtttct
     6301 tgtactctgg atccaaacca tcaatgattt gagtttctcc catcggagcc aaggtcaaga
     6361 ctgaaagagc ttgagtttgc ccacgagtaa agagacctga accatgtaca cgaggaagga
     6421 agtcaacaac cgcatccaaa ggacggattt catcgacctt acgaccatca ggacgcacct
     6481 tgtcttctgt aattaaacgt cgcacttctg cgtgttccat ttgttccaag atttcagcca
     6541 catcacgcat aatacggtca aattcttcgt ggttcgcata tttttcttcg taaacggcag
     6601 tcacttggtc tttcactgct tgagttgcag cttcacgggc caatttctct tctacttgaa
     6661 ctgccttttg gaggtcactg ttgtaggctg caatgatttc agcttgcaat tcagcatcca
     6721 cgtgaagcaa ttccacttct gctttttctt taccgacagc agcaacgatt tcttcttgga
     6781 aggcaatcaa ttctttgaca gcttcgtgcc ctttaagaag cgcttccaac atgatttctt
     6841 ctgacaattc tttggcacca gactctacca tgttgatagc gtgcttggtt ccagctactg
     6901 tcaattcaag aagagattgc tctgcttgtt cttgacttgg gttgatgatg atttggccat
     6961 ctacatatcc cacttgtacc ccagcaattg gtccgtcaaa tggaatatct gaaatagaca
     7021 gtgccaaaga tgaaccaaac atagcagcca ttggtgcaga tgcattttca tcataagaaa
     7081 gcactgtatt gatgacttgg acttcattac ggaaaccttc cgcaaacata ggacgaatcg
     7141 gacggtcaat caaacgcgct gtcaaggtcg catctgttga aggacgtcct tcacgtttca
     7201 taaagccacc aggaaacttc ccagccgcat acattttttc ttcgtagttg acttggagtg
     7261 ggaagaaatc cccagttgcc attttcttag acataacggc agcagtcaag acagttgact
     7321 caccgtaacg tacgacaaca gatccatttg cttgcttagc aacctgacca gtctctacaa
     7381 ttaactcacg acccgcaaaa gtcgtttgaa acacttgttt tgccatttta atcccctttg
     7441 gattgatgaa attatacgcc ttgcctacaa agatcaagat accaaggacg tcaaaagcaa
     7501 agtaaaaata ggaaactgac gaagtcttcg atgaagacaa gacagtttat cttttttaca
     7561 cagcttttcg gccgtgttca attacacaag atattttgga cggttcggct tgccgaacat
     7621 ttctgtagaa aaataggaag gtgacgtcgc actcgacgag tgctaggaag cttatctttt
     7681 ttcctaagaa atgagaccaa aattcaagtc atcaagatac caagccgtca agcaactcaa
     7741 aggaagatag gaaatcgaac gacggagcga ctactcctag ggagatttat ctttttccac
     7801 agagttgtag gcaagttcag ttttcaagat acatcattag aaaggtttaa tactaaagta
     7861 tctaaagctt tcacgctaat cgctatcggg cgattagtta aatgctttac taactctctc
     7921 gtcaaataac atcgatttga ctcactcgtg tcgttaaatc ttacagttta aatgcattgt
     7981 attatttaat accttcatct ttgtatcaag tacgtacaga atttatttta tcatattttt
     8041 cttaaaaagt gaggtcttta ccattaaaaa ggaaccattc ccctcacctg agaagaatgg
     8101 tttgctttta ttatcctaga gactggtgat taaacaaggc atgggttgct tgatggatgt
     8161 attttgctgt atcagcatta ttcatcgtat agagatgcac accggcaaca tcctgagtta
     8221 ccaagtccac gatttggtcc actgcatagg caagtcctgc tgctctgagc gactcagggt
     8281 catgctcata cttgtctaag atggctttaa atttgcgtgg aagatggata ttctcacaag
     8341 tcttcaagag tcggagagcc tgatttcgat tcagaattgg cataattcct gcatgaatgg
     8401 gaacatcaat cccagccaag atgcacttgt cctgaaaatc atagaagcgc tcattgtcaa
     8461 agaagagctg agttacgagg ctcgaacagc ctgcattcac tttcttctta agattttgaa
     8521 tatctgaaat ctgatttggc gaatctggat gcccttctgg atagcaagct ccaataatat
     8581 caaagtgagg ggtttgttcc ttgataaact caatcaagtc tgttgcatag cggaaatcct
     8641 tttgtggttc cacgtctgga ataatatccc cacgaagagc caagattttc tgcaccccaa
     8701 ctttgtccaa gtcagcaata gtttcagcaa ccttgtcctt agttagataa atagctggca
     8761 agtgggcaat ggtcggaatc gccaaatcat tttggataaa gtcagccaaa cgaaccgtcg
     8821 tttccttgat attaaattta ttattgctgg cagttacact gataaaatgg ggagccaact
     8881 cctgcatata ctgcaaggct gaaataatgt taccattacc cacggctggg tttggaggga
     8941 acacttcaaa tgagagtgac ggtgtttggc gtgacatatg taataacctt ttctagttga
     9001 tttctttttg aacaaccact gtatggagag aaatccaatc ttacaatttc tcacgcgcag
     9061 ctttagctgc ttcaacaagg cggatcaagc tttcttttgt ttctgggata ccacgtgttt
     9121 tcaaaccaca gtcagggttg atccaaactt tcttgcttgg cactttagca aggatggctt
     9181 cgattgtgtt gtcgatttcg ccttcatttg gtacacgagg tgagtggata tcgtaaaccc
     9241 caggtcccac ttctgtttgg aagtttttcg ctttgagttc gtccaagatt tcaaggtttg
     9301 aacggctagc ttcaaaggaa ataacgtctg catccatgtt atcgatagct gggatgatat
     9361 ctgtaaattc tgagtaacac atgtgagtgt ggatttgtgt gtctggcgct actgttgagt
     9421 gtaccaagcg gaaggcagga atagcccagt caaggtagtc ttcgtaccag tcgctacggc
     9481 ggagtggcaa tttttcacga agagcagcct cgtcgatttg gatgattttc acaccagcag
     9541 cttcaaggtc aagtacttca tccttgatag caagggcgat ttggagagtt gaatccttga
     9601 tagagatgtc ttcacgtggg aatgaccagt taaggatggt aacaggtcca gtcaacatac
     9661 ctttaacagg tttgtttgta cgactttgtg catagctaga ccatttaaca gtgatagggt
     9721 taagacgagt gacatcaccc cagatgattg gtggttttac cccacgcata ccgtatgatt
     9781 gtacccatcc atttttagag aagaggtatc ctgacaagtt ttgaccgaag tactcaacca
     9841 tgtcattacg ctcaaattca ccgtgaacaa ggacatcaaa gtcaatatct tcttgccact
     9901 tgatccattc gtcaatcgtt tcagcaagga aagcgtcgta ctctttttga gacaattcac
     9961 ctttacggta agccaaacgt ttggcacgaa cttcttttgt ttgagggaat gaaccaatcg
    10021 ttgttgttgg aagagctgga agtttgaaag cttcttcttg gatagcttca cgttctgcaa
    10081 aggctggcaa acgagtgtag tctgcgtctg tcaagccagc gatacgcgca cgaagttcag
    10141 catttttacc aacacgctca gtcgcaaaga gttctttgtt ggctgcaaga gcttctgaac
    10201 cttgaccatt gcggatagca tccaagtcac ggatttcatc caatttttca actgcaaagg
    10261 caaagtggtt caagagtgct ggttcaaatt cttcattagc agttgtaaat ggcacatgaa
    10321 gaagtgagca agagcttgtc aaaacgatgt tttcagctgg gatttgctca agaacagcca
    10381 agctcttttc gtagttgttg cgccagatgt ttttaccatt gacaatacct gcatagagag
    10441 tcttgtcagc tgggaagcca cctttaacga gttcaagagt tttcttacct tcaacaaagt
    10501 caagaccgat agcatctact ggcaagttta caaggtcagc gtatacgtca cgaacatcac
    10561 cgaaataagt ttgaaacaag acttcaagac cttttttgtc agccaagagt ttgttgtaaa
    10621 ggttcaagaa gagagctttt tcttcagctg tcaagtcttt tacaagagcc gcttcatcca
    10681 attggatgcg agtcgcacca aggtcagcca atttagcaaa aacttcttgg taagcagcca
    10741 caaagctatc tacgaagtcg tctgctttca cgccttcttc aaagtctgac aattgaagga
    10801 aagtgaaggg acctacaaga acaggacgag tgttcaatcc aagttctttg gcttcttgaa
    10861 actcatcaaa aatcttgtga cctgcaagtt tgacttgagt gtctttttca aatttaggaa
    10921 cgatgtagtg gtagttagtg ttgaaccatt tcttcattgg aagggcgcga acgtcccctt
    10981 tttctccctg gtaaccacgt cccaaagcga agtagcgctc aaggtcagac aagtccaagt
    11041 tttgaactga ggcaggcact acgttgaaaa ggaaagccgc atctaggaag ttgtcatagt
    11101 gagaaaagtc atttgatggg atttcagtga tgcctttttc tttgacgatg ttccagtgtt
    11161 tagcacgcaa gtcttttgcg gctgcaagga gttcttcttc tgagatttct tttctaaagt
    11221 atttttcagt tgtaaatttt aattcacgga attcgcccaa acgagggaaa ccgatgattg
    11281 tagttgacat gatgtgtcct ccaaaatttg ttgttgaaac tatcttaaca gaaaagaaag
    11341 cgtctgtata attgtaaaaa attaggcttt gatatagttt gaaactatat atctgtttcg
    11401 gacaaaagaa aaagacttga agcaaacgtc tcaaatcctt tgtaattctt actttacagc
    11461 tatattccaa ttagaatact aaaacatgtt attagtaatt cttataagtg actatgacct
    11521 gttattagaa aagactataa ctgattctag tcaacttttt ccctgttcaa gtgggacgat
    11581 tgctagtgtc tttcctaaac tggctaggac ttttaagact gtatccaact gaggactagt
    11641 ctttcctgtc tccatcctag ctatgacagg ctggcttatt ccactgactt cttccagctt
    11701 tttctgactg attccttgtt catacctagc ctcaatcaac tcgctcatga tagccactcg
    11761 catatcactt tcaaggattt cctccttgct aaagagctca gatggacatc cttccaatta
    11821 ctcccaatag cactattctt catcacttaa ccctctttct tttacgtcta tgtattttaa
    11881 aaaaaatgag cgaattatga ttcgatagat tgaccagcgg gtttaaagtt ggtgctagcc
    11941 tatttcttaa gcgattttcc ttttctagga taaagcagat cctgcttgct taaccccaat
    12001 tttccacgat gaatccaata gtaaatggtt gaaattccca cgttaacccc tttagccatc
    12061 accatcattt caggcgaaaa tttttggtta tgtatagtgg agaatctttt cctttagttc
    12121 cttggtcaag cttgatttct tgaccgagcg cttgcgattg ttttcataag actgttgagc
    12181 gtagtcggca gaataaacct ctttgaagcg cccttttcca agacattttc ggactgtccc
    12241 acgcttgatt tcagtgtgga tagtttgagg agcttttcca agtagagagg caacttctct
    12301 atttgatttt ccttcttttt tccatctttc gattaagcga cggctatcga ttgtcaaatg
    12361 tttacctttt gtagtataat tgtcttgcat ctctgtgcct tttaatcatt tcaatcttaa
    12421 attggacttt ttttacttgg gttgtactta atctataagg aagacaagaa aaagaatatc
    12481 aatcaagtaa agtcacaaag tcacattagc tccgagcaac cattgcaaat tgaggtactc
    12541 acacaatgat taaaacattt ctctctgccc tttcggtcat tctctttgtt atccctatca
    12601 taacttattc tttttttcca tcttctaatc ttaatatttg gctatctacc agacctatct
    12661 tggcacagat ttatgccttc ctcttagcta ctgcaactat ggctgccatt ttaagtttcg
    12721 tatttttttc cctatctttt tacaagaaaa ataaacaaat acggttttac tctggcattt
    12781 tgctcttact atcgctcata ttactattat tcggaacaga taaaaccctt tcttctgcat
    12841 caaataagac taaaacctta aaattagtaa cttggaacgt cgctaatcaa atagaagcac
    12901 aacatattga gcgaattttt agccattttg atgccgatat ggctatattc cctgaactag
    12961 ctaccaatat cagaggtgag caagaaaacc agagaatcaa actattgttt catcaagttg
    13021 gactttctat ggccaactat gatattttca cttctccacc taccaatagt ggaatagctc
    13081 ctgtgactgt gattgtcaag aaaagttatg gtttctatac agaagctaaa acttttcata
    13141 caacacggtt cgggacaatt gtattacatt cgagaaaaca aaatatacca gatatcattg
    13201 ccttgcatac tgctcctcct ctgccaggtt taatggaaat ctggaagcaa gacttaaaca
    13261 tcattcataa tcaattggct tcaaaatatc caaaggctat tattgcaggt gattttaatg
    13321 caactatgcg tcatggagca cttgcaaaaa taagctctca tagggacgca ttgaatgcac
    13381 ttccaccttt tgaaagagga acttggaata gccaaagtcc aaaacttttt aatgcaacga
    13441 tagatcatat cttattgcct aaaaaccact actatgttag agatttagac attgtaagtt
    13501 ttcaaaactc tgatcataga tgtattttta cagaaatcac attttaatta ttttatataa
    13561 aatcacccct ctagtgttca taaactagag ggggaatttg tatcatacta tcgtttaacg
    13621 cacttctgca ttgacttttt cttcgagcga tgcttggatt ttttccatat agcgtgcgac
    13681 ttcttcgtcc gttaagctat cttctggatt ttggaaggtc aagctataag ccattgactt
    13741 cataccaagt cccaatttct cacctgagaa gacgtcaaag agtttgatat ctgtcaaacg
    13801 tttcacgccg gcagcttgga tagcatctac aacttcttga tgagtcactt ctgccttgag
    13861 gagaagggca acgtcacggc tgactgccgg gaatttggtg atttctacaa atggagtcgc
    13921 tggctgaagc gcagcttcga tagctgaaag attaagctca gccacatacg tttctggaat
    13981 atcgtaagcc ttggcagtga ctggatgcac ttggccaagg aaaccaagaa cttggtcacc
    14041 gaatgaaatc acggctgtac gacctggatg aaggctagcg atttcagatg ttgctgtata
    14101 ggttacttgg agtcccaaac gagtaaatag ggcttcaagg attcccttag catagaagaa
    14161 atcaactgga actgctgctg tttggaaatc tttttcagca accaagcctg tcaaggcaaa
    14221 ggcaaaactg ttgatttcat ttggaagttc ttcttttgga ttacctgttt gttcaaagac
    14281 ttttccaatc tcgtaaaggg ccaagttttt attcttacga gccacgttgt aggcaacggt
    14341 atcaaggata cctgaaatca tattttgacg gaggactgaa cgatccacag tcattggcca
    14401 catgagttcc gtaaggttac ttggttgagc cgtaaactca actgcttttt caggagttgt
    14461 tagagtatag gtgatgattt ctgtcaaacc tgctccttca gcaatagtac gaacttgacg
    14521 gcggagtttt tgtgtggctg tcaattcacc tgctgtacca tcgtcttttg gtagactagt
    14581 tggcaagcgg tcataaccat agatacgagc aatttcttca aagaggtcag cttcgattgt
    14641 gatatcccaa cgacgacgtg ggactctgac tgtaaagctg tctgcatttc cagaaagacc
    14701 aaagccaaga cgacggaaga cgtcttctac atcagcataa gacagctcag ttccgaggac
    14761 acgattaaca tcagcaaggg ttgaagaaac ttctacatct gaagtatcaa gctcacccgc
    14821 tgaaacgatg cccttacgca ccgtcgcacc tgcaagttcc gcaatcaggc tagctgccgc
    14881 atcaagggct tcattaactg ttgccacatt aattcctttt tcaaagcgag aagatgactc
    14941 agaacgaagg ttcaggcgac cacttgtctt acggatagat ttgccattga aaacagcagc
    15001 ttcaaggaca acacgactag atttttcaga gatttctgtt gcttgaccac ccatgacacc
    15061 tgcaagggct actggcttgt ctgcgacagt gatgactagg tcattcacgt ccaagtcacg
    15121 ttcttcaccg tccaaggtca ccaatttttc accagcacgc gcttcacgca cacggatgtc
    15181 agtcccttca aagttatcca agtcaaaggc atgcattggt tgaccaaaat agagcaggat
    15241 gtagttggtc acgtccacta cgttattgat gggacggatt ccttcgttca tgagaagatt
    15301 ttgcaaccat tgtggacttg gtgcgatggt cacattgtcc aagatacgag ctgcatagta
    15361 aggcgccttg tctgtctcaa tgctgacaga aagggcatct tccgcagctt cattagtttc
    15421 tgttagagta aattctttaa agttgactgc cttgtcatag atggctgcca cttcgtgagc
    15481 cactccacac atagaaaggg catctgcacg gtttggtgtg atggaaagtt cgatgatttc
    15541 atcatccaag tctaggtaag aaaagacttc ctcacctggc acggcatctt caggcaagat
    15601 ttggatgcca tctgcgaatt ccttaggcac aactgagtca gaaattccca attcaccaag
    15661 tgaacagatc attccaagtg actccaaacc acggattttt ccttttttga ttttgtagtt
    15721 atcagcgata cgagctcctg gaagagccac catgaccttg atcccagcac gcacatttgg
    15781 ggcaccacaa acgatctgac gctcttcttc gccaacgtta acctgacaaa catggaggtg
    15841 agtctctggc acatcttcgc aagacaagac ctcaccgacg acaatttttg agagaccagc
    15901 agctggtgat tcgacaccct ctacctcgat tcctgtagtt gacatttttt cagccaactc
    15961 ttgtgatggc acatcaatgt ccaccaattc ttttaaccat ttataagata taagcataat
    16021 ttagttctcc agaatgacca ttgccacact ggttcttttc ctttcctatc atttcaatag
    16081 aagaatcctc ttcttacctt aatttctttc tcagtaacca atccgtatct actttttgac
    16141 caaccataaa atgatgttgg ctaaattttt caaaaccata tcgattataa aacgcttgag
    16201 cttttgtatt atgctcccaa acacctagcc aagcccaaga aaaactattt tttgtagcaa
    16261 gttcaagtgc gaattcaaac agttgcttac ctagtccaaa tccttggaat ttttgtagca
    16321 catagaggcg ttgaatttca aaagcgtcct ctaattctct ctcagtttga gcacttcccc
    16381 agttgacttt gagaaaacca gctatctcct cctcatgcat aatgaaatag gtttcagagt
    16441 caggatttcc caactcagtt gacaaagttt tcagactata agcctcttca aagtattcct
    16501 gtaactgctc ttccgtatta tcatacgcaa aggtttcacg aaaggcttgt ttggcaattt
    16561 tagccaacac ctcaacatct gccatttcta cttttctaat cattatttaa actgttctga
    16621 gaagcggaca tctccttggt agaatccacg gatatcgttg attccataac ggagcatagc
    16681 tacacgctct tgtccaagac caaaggcaaa accagagtat acagtcgcat cgataccact
    16741 catttcaagg acacgtgggt gaaccatacc ggctcccata atttcgatcc aacctgtttt
    16801 cttacatacg ttacagcctt ctccaccaca cttgaagcaa gaaacatcca cctcaacaga
    16861 tggctctgtg aatgggaagt aagatggacg caaacgaatt tgacgctctt caccaaacat
    16921 tttttggaca atcaactgaa gcgttccttg aagatcagcc atagagatat ttttcccaac
    16981 taccaagcct tcgatttggt ggaattggtg actgtgggtc gcatcgtccg tatcgcgacg
    17041 gaagacacgc cctggcgaga tcatcttcaa aggaccttta gaaaaatcat gggcatccat
    17101 agcacgcgcc tgaactggag acgtgtgggt acggagcaag atttcttcag tgatatagaa
    17161 agtatcctgc atatcacgag ctgggtggtc ttttggaagg ttcatacgtt caaagttata
    17221 gtagtcttgc tccacttcaa aaccatccac gacttgataa cccataccga tgaagatatc
    17281 ttcgatttct tcactggttt gtgtcaaaac gtgacggtga ccagtcgcaa ctggacgacc
    17341 tggaagcgtc acatcgatac tctcgctagc cagttgagcc gcgactttct tttcttccaa
    17401 gagcttagct gtttcttcaa aagcagctgt caagacatca cgagcttcat tgacgtgttt
    17461 cccgatgatt ggacgcatct cagcagaaac atctttcatc cctttgagga tttcagtgag
    17521 cgaacccttt ttaccaagga cagagacacg caaatcttgc atctcttttt catttccagc
    17581 agtaatctgc ttcaagctag tcagcgtttc ttcgcgaagc gcttttaatt gttcttcaat
    17641 agttgacata tttcctccat cagtctctcg tagataaaaa gaaaaccaca tgccaaaaac
    17701 tccactcgga gcgttgacac gcggtaccat ccgttttcat ctgacaagtc agaccttcat
    17761 ttctaaatcc atgcgcaagt gaattcaccc agctttcata tagagagctt gcagtcacgg
    17821 ctctcctccc tgatatactt cccttgggct actagtcttt cagatttcta ttcaattacc
    17881 acttagttta tcagattttt accattcttg caagacctat cttacttctg cttgttagct
    17941 tattcttatc taactttata gcggattgaa ataagatga
//
LOCUS       DAVMGZ010000029        16543 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000029 DAVMGZ010000000
VERSION     DAVMGZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.030"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            98..1123
                     /gene="trpS"
                     /locus_tag="VYQ93_000816"
     CDS             98..1123
                     /gene="trpS"
                     /locus_tag="VYQ93_000816"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV3335536.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1275..2753
                     /gene="guaB"
                     /locus_tag="VYQ93_000817"
     CDS             1275..2753
                     /gene="guaB"
                     /locus_tag="VYQ93_000817"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV3335537.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2804..3901)
                     /gene="recF"
                     /locus_tag="VYQ93_000818"
     CDS             complement(2804..3901)
                     /gene="recF"
                     /locus_tag="VYQ93_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV3335538.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(3904..4272)
                     /gene="yaaA"
                     /locus_tag="VYQ93_000819"
     CDS             complement(3904..4272)
                     /gene="yaaA"
                     /locus_tag="VYQ93_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV3335539.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4431..5681
                     /locus_tag="VYQ93_000820"
     CDS             4431..5681
                     /locus_tag="VYQ93_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV3335540.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            5678..6961
                     /locus_tag="VYQ93_000821"
     CDS             5678..6961
                     /locus_tag="VYQ93_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV3335541.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTLVTEVDGDVKQYPAGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESVSQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKLTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIYDGDIVDFTIFPS"
     gene            7003..7824
                     /locus_tag="VYQ93_000822"
     CDS             7003..7824
                     /locus_tag="VYQ93_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3335542.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7835..8380
                     /gene="pgsA"
                     /locus_tag="VYQ93_000823"
     CDS             7835..8380
                     /gene="pgsA"
                     /locus_tag="VYQ93_000823"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV3335543.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8377..9204
                     /locus_tag="VYQ93_000824"
     CDS             8377..9204
                     /locus_tag="VYQ93_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV3335544.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKHGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9189..10028
                     /locus_tag="VYQ93_000825"
     CDS             9189..10028
                     /locus_tag="VYQ93_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV3335545.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRIFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10021..10815
                     /locus_tag="VYQ93_000826"
     CDS             10021..10815
                     /locus_tag="VYQ93_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV3335546.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10874..11692
                     /gene="mreC"
                     /locus_tag="VYQ93_000827"
     CDS             10874..11692
                     /gene="mreC"
                     /locus_tag="VYQ93_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV3335547.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11692..12186
                     /gene="mreD"
                     /locus_tag="VYQ93_000828"
     CDS             11692..12186
                     /gene="mreD"
                     /locus_tag="VYQ93_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV3335548.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12280..13458
                     /locus_tag="VYQ93_000829"
     CDS             12280..13458
                     /locus_tag="VYQ93_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV3335549.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13682..14461
                     /gene="rpsB"
                     /locus_tag="VYQ93_000830"
     CDS             13682..14461
                     /gene="rpsB"
                     /locus_tag="VYQ93_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV3335550.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            14540..15580
                     /locus_tag="VYQ93_000831"
     CDS             14540..15580
                     /locus_tag="VYQ93_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV3335551.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            15826..16149
                     /gene="tnpB"
                     /locus_tag="VYQ93_000832"
     CDS             15826..16149
                     /gene="tnpB"
                     /locus_tag="VYQ93_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV3335552.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDVKALTSEQVDWLMKDFS
                     ITPQI"
     gene            complement(16290..16522)
                     /locus_tag="VYQ93_000833"
                     /pseudo
     CDS             complement(16290..16522)
                     /locus_tag="VYQ93_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         5176 a         2636 c         3508 g         5223 t
ORIGIN      
        1 aaatctgtaa aaagcaaggc tgatttattt tgataaatta cggttatttc attaaaaaaa
       61 tgctataatt gaaaggacta tatcgaagga gaacaaaatg actaaaccca ttattttaac
      121 aggagaccgt ccaacaggaa aattgcatat tggacattat gttggaagtc tcaaaaatcg
      181 agtattatta caggaagagg ataagtatga tatgtttgtg ttcttggctg accaacaagc
      241 cttgacagat catgccaaag atcctcaaac cattgtagag tctatcggaa atgtggcttt
      301 ggattatctt gcagttggat tggatccaaa taagtcaact atttttattc aaagccagat
      361 tccagagttg gctgagttgt ctatgtatta tatgaatcta gtttcgttag cacgtttgga
      421 gcgaaatcca acagtcaaga cagagatttc tcagaaagga tttggagaaa gcattccgac
      481 aggattcttg gtctatccaa tcgctcaagc agctgatatc acagctttca aggctaatta
      541 tgttcctgtt gggacagatc agaaaccaat gattgagcaa actcgtgaaa ttgttcgttc
      601 ttttaacaat gcatataact gtgatgtctt ggtagagccg gaaggtattt atccagaaaa
      661 tgagagagca gggcgtttgc ctggtttaga tggaaatgct aaaatgtcta aatcactaaa
      721 taatggtatt tatttagctg atgatgcgga tactttgcgt aaaaaagtaa tgagtatgta
      781 tacagatcca gatcatatcc gcgttgagga tccaggtaag attgagggaa atatggtttt
      841 ccattatcta gatgtttttg gtcgtccaga agatgctcaa gaaattgctg atatgaaaga
      901 acgttatcaa cgaggtggtc ttggtgatgt gaagaccaag cgttatctac ttgaaatatt
      961 agaacgtgaa ctgggtccta ttcgtgagcg ccgtattgaa tttgctaagg atatgggaga
     1021 agtttataat atgattcaaa aaggtagtga aagagcgcgt gaagttgcgg gtcaaaccct
     1081 atctgaggta aaaggggcaa tgggacttca ttactttaac taagtttatt ttacaagaaa
     1141 ctatcttttc tatctcgaag aaaagatagt tttttattta cccctgtaac atttgacaaa
     1201 tttttataga atggtatgat agatacaata taaaaagagt caagctcaaa aagaaagaaa
     1261 agaggaaact tcgaatgtct aattgggaca ctaaattttt gaaaaaaggt tttacctttg
     1321 atgatgtatt gcttattcca gctgaaagtc atgtgttgcc taacgatgca gatttaacaa
     1381 ctaaattggc agataatttg actttaaata tcccaattat taccgctgcc atggacacag
     1441 ttacagagag tcaaatggcc attgctattg ctcgtgcagg cggtctcgga gttatccata
     1501 aaaacatgtc aattgctcaa caagcagacg aggttcgtaa ggtaaaacgt tctgaaaatg
     1561 gagttattat tgatccgttc ttcttgacgc ctgaacatac aattgctgaa gcagatgagc
     1621 ttatgggtcg ttaccgcatc agtggtgttc cagttgttga aacacttgaa aatcgtaaat
     1681 tggttggtat tttgacaaac cgagatcttc gttttatttc agattataat caaccaattt
     1741 caaaccatat gactagtgaa aatcttgtta ctgctcctgt gggtacggat cttgcaacgg
     1801 ctgagagtat tcttcaagag catcgtattg aaaaacttcc gttggtcgat gaagaaggca
     1861 gtctttctgg tttgatcact atcaaagata ttgaaaaagt tattgagttt ccaaatgcgg
     1921 ctaaagatga gtttggtcgt cttctagttg caggtgcagt aggtgttact tcagatacat
     1981 ttgaacgtgc agaggctctt tttgaggcag gagcggatgc gattgttatt gatactgcac
     2041 atggtcattc tgcaggtgtc ttgcgtaaaa ttgccgagat tcgtgctcat ttcccagatc
     2101 ggactttgat tgctggaaat attgctactg ctgaaggtgc acgtgccctt tatgaagcgg
     2161 gtgtagacgt tgttaaggtt ggtattggac caggttctat ctgtactact cgtgtgattg
     2221 ctggtgttgg tgttccgcaa gtaacagcta tctacgatgc tgcagctgtt gcgcgcgaat
     2281 atggtaaaac gattattgct gacggtggga tcaagtattc tggagatatt gtaaaagcac
     2341 ttgctgcagg tggaaatgct gttatgcttg gatctatgtt tgctggaact gatgaagctc
     2401 caggcgaaac tgaaatcttc caaggacgta aattcaagac ttaccgtggt atgggatcaa
     2461 ttgctgctat gaagaaaggt tcaagcgacc gttatttcca aggttctgtc aatgaagcaa
     2521 acaagcttgt tccagaagga attgaaggtc gtgttgctta taaaggagcg gcagctgata
     2581 ttgttttcca aatgattggt ggtattcgct ctggtatggg ttactgtggt gcagctaacc
     2641 ttaaagaact acacgataat gctcaattta ttgaaatgtc tggtgctggt ttgaaagaaa
     2701 gccatcctca tgatgtgcaa attactaatg aggcaccaaa ttattctatg taaaaaacaa
     2761 tgaaaagaac tccagtgaaa acaggagttc ttttacaatg ttgtcaattt ccatttacag
     2821 aagctttacc atcctgaata gtgaagatac ttagattttc tggcagattt tgaagatggt
     2881 ctaagcttgt tgttgtgata aaggtttgga ttgattgaga aatcgtttct aataatttta
     2941 actgtctagt gttgtcaagt tcactcatca catcgtcaag cagtaatatc ggagattctg
     3001 tggtaatgct ttccattaat tcgatttctg ctaattttat cgagaggacg agactacgat
     3061 gttgaccttg gcttccgaaa ctagcatcca tcccatttat ataaaaagaa atgtcatctc
     3121 gatgaggacc gacaccagta ttctttttaa ataaatctct ggatctactt ttttctaaag
     3181 caattttgaa agattcggat aagttttgtt tgtcagttat attgacagaa gattgatagg
     3241 atattgacaa ctcttcgatc tgattagaga gttcaaaatg tttcttacgc ccaaatgatt
     3301 ctagtttttt tatgaaatct aagcggtgat tcattacacg acatccataa tcaactagct
     3361 gatcatctaa cacagaaagg aatgtttcat ctattttttg atctgatttt aggtaagtgt
     3421 ttctttgctt taggatgtgg ttataattgg ttaagtcaga taagtagatt ggcttaattt
     3481 gcccaagttc catatcaatg aattttcgtc gaatcgaagg tgctccttta attagttgta
     3541 aatcttcagg agcaaataag acaacattca tgtgtcctac ataatctgaa aggcgtgcct
     3601 gttttaagtg attaactttt gttacacggc ctttttgtgt tagttcgatt tctaggggaa
     3661 tggatccagt ttttttctga acgagacctg aaagatgaag ttgttcctca tcaaaatgaa
     3721 tgagattttt atctgttcga gttcgatgac tacgcgttaa ggctaaaaaa tagatagcct
     3781 ctaacatgtt tgtttttcct tgtgcattac gtcccaaaaa gacattcaat ttaggattaa
     3841 agtctatttt cgtttctttg taattacgaa aagtcttgag agatagatgt tgtagccaca
     3901 tgattatcta ccagggaatc gtggagcttg ttggctttta ggtgatgaag caggtttcga
     3961 tttgtctttt ttaactccct tattcatctt tttgacaagt ttagcgatcc gttctttttc
     4021 aactttatca gcttggtatt catcttgttc ttcagaagta ggttgtgtca acaagatgtc
     4081 aatgttcatg tcagggatgt caactttatc gccaatacga agtttttttc cacgacgact
     4141 ctctaattcc ctattaaagt aaacagaatg ttcagagaga aatgatttaa tagctcctcc
     4201 gctatgtgta attccaagtt ctttgagtag tgcttggagg gtaataaatt cttcaaataa
     4261 tttgtattcc ataattcacc tcaatctttg gtattatacc atattttcct aaaaatagtg
     4321 agagagacag cgagaaatgt aagcgatttt tatttcaaaa gtgttacagg gaacaagaaa
     4381 atttcaggtt ttcgtgatat aatagaagtc tgtatataag gaggtaaatc atggagttag
     4441 tgcatggaat ttcaacacat tttatccaat caaaaaagtt taaaacaaac aaaattaccg
     4501 tgcgttttac cgctccatta tcccttgata cgattgcagg tcacatgttg agtgcaagta
     4561 tgctagagac tgctaatcag atgtacccca cttctcaaga tttgaggaga cacttggcca
     4621 gtctatacgg tacagatatg tcaaccaatt gtttcagaag agggcaaagc cacattatag
     4681 aattgacatt tacctatgtt cgtgatgagt ttttaagtag gaaaaacgtg ctaacctctc
     4741 agattttgga acttgtaaaa gaaactcttt tttcacccgt agtagttgat aatgggtttg
     4801 atccggcctt atttgaaatt gagaaaaaac aattgctagc aagtttagca gctgatatgg
     4861 atgattcttt ttattttgca cataaagaat tggataaatt gttttttcat gatgaacgtc
     4921 ttcaattgga atatagtgat ttacgaaatc gtattttagc tgaaactcca caaagttctt
     4981 attcttgttt ccaagaattt ttagccaatg atcgaataga tttctttttc ctaggtgatt
     5041 ttaatgaggt tgaaattcaa aatgtattag aatcatttgg ctttaaaggt cgaaaaggag
     5101 atgtgaaggt tcagtattgt caaccttatt ctaatatcct tcaggaaggt atggttcgga
     5161 aaaatgtggg acaatctatt ttggaattag gttatcatta ccgttctaaa tatggtgatg
     5221 agcaacattt acccatgatt gtaatgaatg gtttacttgg tggatttgct cactctaagc
     5281 tctttacaaa tgtccgtgaa aatgctggat tagcttatac catttcaagt gagcttgatt
     5341 tatttagtgg attcttgagg atgtatgctg gtatcaatcg agaaaatcgt aaccaggctc
     5401 gtaaaatgat gaataatcaa ctgcttgatt taaaaaaagg ttattttaca gagtttgagt
     5461 taaatcagac caaggaaatg attcgttggt cgttgttact ttctcaagat aatcaatctt
     5521 cattgattga acgtgcttat caaaatgcct tatttggaaa atcttcagca gactttaaaa
     5581 gttggattgc aaagcttgaa caaattgaca aagatgctat ttgtagagta gctaataatg
     5641 tgaaactaca agcgatttac tttatggaag gaatagaatg acaaaggttg tttttgaaga
     5701 aaaatactat ccagctgtaa aagaaatggt ttatcgaact cgtttggcaa atggattgac
     5761 agtagctctt ttgcctaaaa aggaatttaa agaggtttac gggagtgtaa ctgtacagtt
     5821 tggttcggta gatacgcttg tcacagaagt tgatggagat gtaaaacaat atcctgcagg
     5881 aattgctcat tttcttgaac ataaattatt tgaaagagaa gattctagcg atttgatgtc
     5941 ggcttttacg agtctaggtg cagatagtaa tgcctttaca agctttacaa aaacaaacta
     6001 tcttttttca gcaacggatt attttttaga aaatttagat ttacttgatg aattggtaac
     6061 atcagcacac tttactgaag cttccattct gacagagcag gatattattc agcaagaacg
     6121 agaaatgtac caagatgatc cagattcgtg tttattcttt tcaactttag cgaatttgta
     6181 tcctggtaca cctttagcaa ctgatatagt tggaagtgag gagtccgttt cccaaatcaa
     6241 tctaactaat ttgcaagaaa attttacaaa gttttacaaa cctgtaaaca tgtctctgtt
     6301 tttagttggt aattttgatg tggagcgagt acaggactat tttgaaagca aagaactgaa
     6361 agattcagat tttcaggaag tagcaagaga aaagttgttt ttacagcctg taaagctaac
     6421 agatagtatg agaatggaag tatcttctcc caaactagcg attggagtta gaggtaagcg
     6481 agaagtttct gaagcggatt gctatcgaca tcatatttta ttaaaattat tgtttgcaat
     6541 gatgtttggt tggacttcgg atcgttttca aaaatgttat gaatcaggta aaattgatgc
     6601 atccttatct ctggaagttg aagtaacaag tcgctttcat tttgtcatgt tgacaatgga
     6661 tacgaaagag ccagttgctt tgtctcatca atttaggaag gctattcgta attttacaaa
     6721 ggatttagat attacagagg aacatttaga tattatcaaa agagagatgt ttggcgaatt
     6781 tttcagtagc atgaactctc ttgaatttat tgcaacgcaa tatgatgctt ttgaaaatgg
     6841 tgagacaatt tttgatttgc cgaaaatttt acaggaaatt actttagagg atgtccttga
     6901 tgctggacat catttaatat atgatggtga catagttgat tttacaatat ttccatcgta
     6961 gtaacctatc ataatagaca ctagaaagaa gggatgacaa gtatgagaaa aaaaacaatt
     7021 ggagaggttt tacgattagc tagaatcaat cagggattga gtttagatga attgcagaaa
     7081 aagacagaaa tccagttaga tatgttggaa gcaatggaag cagacgattt cgatcaactt
     7141 ccaagtcctt tttacacgcg ttctttcttg aaaaaatatg catgggctgt tgagttagat
     7201 gaccaaattg ttttggatgc ttatgattct gggagtatga ttacttatga ggaagtagat
     7261 gttgatgaag atgagttgac aggtcgtaga cgttcaagta agaaaaagaa gaaaaaaaca
     7321 tcatttttac ctttatttta ttttatcctt tttgctttat cgattttaat ttttgtgact
     7381 tattatgttt ggaactatat tcaaactcaa ccagaggagc cttctctttc taattacagt
     7441 gtggttcaat caacaagttc aactagctct gttccccact cctcaagtag tagttcttct
     7501 agtatagaat cagctataag tgtatcaggc gaaggaaatc atgtagaaat cgcttataag
     7561 acaagtaagg aaacagttaa attgcaattg gcagtttcag atgttacaag ttgggtcagt
     7621 gtttcagaaa gcgaacttga gggcggtgta accttatcgc caaagaagaa aagtgcagaa
     7681 gcaacagttg caactaaaag tcctgtaaca attacgttag gtgttgtaaa aggtgttgat
     7741 ttgacagtag ataatcagac tgttgattta tcgaaattaa cagctcagac tggacaaatc
     7801 actgtaacct ttactaaaaa ttaaggaaaa acgaatgaaa aaagaacaaa ttcccaatct
     7861 cttaacaata ggtcgaattc tctttatacc tatttttatc tttattttaa cgataggaaa
     7921 ttcgatagag agtcatatag ttgcagctat tatctttgct gttgccagta ttaccgacta
     7981 tttagatgga tatttagctc gtaaatggaa tgtggtcagt aattttggta aatttgcaga
     8041 tcctatggcg gataagttac tagttatgtc ggcttttatt atgttgattg agttaggtat
     8101 ggctccggct tggattgttg cagtgattat ctgtcgtgag ttagctgtga caggtttaag
     8161 gcttttattg gttgaaactg gtggaacaat tttagcagca gcaatgcctg gaaaaattaa
     8221 aacttttagt cagatgtttg ctattatttt cttgctatta cattggactt tgcttggtca
     8281 agttctactt tatgtagcct tatttttcac tatctactct ggctatgact atttcaaggg
     8341 tagtgcctat gtatttaaag ggacatttgg ttcgaaatga aatcaataat tgatgtaaaa
     8401 aatctttctt ttcgctataa agaaaatcag aactactacg atgtgaagga tattacgttt
     8461 cacgtgaaac atggagaatg gctttcgatt gtagggcata atggtagtgg taaatcaacg
     8521 acggttcgat taattgatgg cttactggaa gcagaatccg gagagattgt aattgatggc
     8581 caacgtttga ctgaggaaaa tgtttggaat atacgtcgtc aaatcggtat ggtttttcaa
     8641 aatccagaca atcaatttgt tggagcgact gttgaagatg atgttgcttt tggtttggaa
     8701 aatcagggac tttctcgtca agaaatgaaa aagagagtag aagaagctct ggctttagtt
     8761 ggcatgttgg actttaaaaa gagagagcca gcgcgtctat caggtggcca aaagcaacgt
     8821 gtggccattg caggtgttgt agccctaaga ccagctattt taatcttaga tgaagcaacg
     8881 agtatgttgg atcctgaggg gcgtagagaa cttattggga cagtaaaagg aattcgaaaa
     8941 gactatgata tgacggtcat ttctattacc catgatttgg aagaagtcgc catgagtgat
     9001 cgcgtattgg tcatgaaaaa aggggaaatt gaatcaacta gtagtccaag ggagcttttc
     9061 tctcgaaatg atttagatca aattggatta gacgatcctt ttgctaatca attaaaaaaa
     9121 tctttgagcc agaatggcta tgatttacct gaaaattatt tgacagaaag tgagctagag
     9181 gataagctat gggaattgct ctagaaaatg tgaattttac atatcaagaa ggtactccct
     9241 tagcttcagc agctttgtcg gatgtttctt tgacgattga agatggctct tatacagctt
     9301 taattgggca cacaggtagt ggtaaatcaa ctattttaca actcttaaat ggtttattgg
     9361 tgccaagtca agggagtgtg aggatttttg ataccttaat cacctcgact tctaaaaata
     9421 aagatattcg tcaaattaga aaacaggttg gcttggtatt tcagtttgct gaaaatcaga
     9481 tttttgaaga aacggttttg aaggacgttg cttttggacc gcaaaatttt ggagtttctg
     9541 aagaagatgc tgtgaagact gcgcgtgaga aactggctct ggttggaatt gatgaatcac
     9601 tttttgatcg tagtccgttt gagctgtcag ggggacaaat gagacgtgtt gccattgcag
     9661 gcatacttgc catggagcca gctatattag tcttagatga gccaacagct ggtctagatc
     9721 ctctagggag aaaagagttg atgaccctgt tcaaaaaact ccaccagtca gggatgacca
     9781 tcgtcttggt aacgcatttg atggatgatg ttgctgaata tgcaaatcaa gtctatgtaa
     9841 tggaaaaggg acgtttagta aaggggggca aaccaagtga tgtctttcaa gacgttgttt
     9901 ttatggaaga agttcagttg ggagtaccta aaattacggc cttttgtaaa cgattggctg
     9961 atagaggcgt gtcatttaaa cgattaccga ttaagataga ggagttcaag gagtcgctaa
    10021 atggatagta tgattttggg gcgttatatc ccaggggatt cgattgttca ccgattggat
    10081 ccacgtagca aattgcttgc tatgatgcta ctgattttga ttgtgttttg ggctaataac
    10141 cccttgacga atctgattct ttttatagcg acagggatat ttattgcctt gtcaggagta
    10201 tctctttcat tttttattca gggcttgaag tctatgtttt tcttgattgc cttcacaact
    10261 atttttcaac tatttttcat ttctaatggg aatgttttat ttgagttttc gtttgtgaga
    10321 atcacggatt atgctttgca acaagctggg attatttttt gtcgctttgt attgattatt
    10381 ttcttttcaa ctttgttaac cttaacaacc atgcctttaa gtttggcatc agctgtcgaa
    10441 gctttattag cacctttaaa gcgtgtgaaa gttccagttc atgaaattgg attgatgctg
    10501 tctatgagtt tgcgttttgt cccaaccttg atggatgata cgacgcggat tatgaatgca
    10561 cagaaagctc gtggagtgga ttttggagaa ggaagtatcg ttcaaaaagt aaaggcgatg
    10621 attcccattt tgattcctct ttttgcgaca agtttaaaac gtgcagattc cttggctatc
    10681 gctatggaag cgcgtggcta tcagggtgga aaaggcagaa gtcaataccg acaattgaaa
    10741 tggactctaa aggatacgct gaccattctt gttattctcg tacttggttg ttgtttattt
    10801 ttcttaaaat cttagtagct ttcaggaatt gataaaaagt tactgtaaca gtttttgata
    10861 taaaggtagg gatatgaacc gttttaaaaa atcaaaatat gtcattattg tttttgtcac
    10921 tgttctgctt gtgtcagctc tcttagcgac gacttattca agtacaattg tgacaaaatt
    10981 aggagatgga atctcattgg ttgatagagt tgtacaaaaa ccttttcagt ggtttgattc
    11041 tgtcaaatca gatttggctc atttgacacg aacatataat gaaaatgaaa gtttgaagaa
    11101 acagctttac caattagaag ttaaatcaaa tgaggtggaa agtttaaaga cagaaaatga
    11161 acaactgcgc caattgcttg atatgaagtc taaattgcaa gccacaaaga ctttagcagc
    11221 agatgttatt atgcgttctc cggtatcttg gaagcaggag ttgaccttag atgcaggtag
    11281 atcaaaaggt gcttctgaga acatgttagc tattgcaaat ggtggcttga ttgggagtgt
    11341 ttcaaaagta gaggagaact ctactatagt caaccttctg acaaatacgg aaaatgctga
    11401 taagatttct gttaaaattc aacatggctc tactacaatt tatggaatta ttattggcta
    11461 tgacaaggaa aatgacgttc ttaaaattag ccaattaaat agtaatagcg atattagtgc
    11521 gggagataag gtgactactg gtggattagg aaactttaac gttgctgata ttcctgttgg
    11581 tgaagtggtt gccacaacgc atagtacaga ctatttgaca cgagaagtaa ctgttaaatt
    11641 gagtgcagat actcataatg tagatgtgat agaattagtg gggaattcat aatgagacag
    11701 ttgaagcgag ttggagtatt tttattgctt cctttctttg ttctaattga cgcccatatt
    11761 agccagcttc tgggctcatt tttcccccat gtacatttgg ctagtcattt tctttttcta
    11821 tttctcttat ttgagacgat agaagtatca gagtatctct acctagtcta ttgttttgtt
    11881 ataggcttgg tttatgatgt ttactttttc catctaatag ggattacaac tctcttattt
    11941 atcttattgg gagccttcct tcataaattg aatagtgtta ttttgttgaa tcgttggaca
    12001 agaatgctag ctatgattgt gctgacattc ctgtttgaaa tgggtagtta tcttttggct
    12061 tttatggtag ggttgacagt agatagcatg tcgattttta tagtctatag cttggtaccg
    12121 acgatgattt taaatttttt atggattact gtttttcaat ttatttttga aaaatattat
    12181 ctataagaac gacatataaa tgtaacaaag gcgtaatatt tattaggcct ttttttggta
    12241 tactagtatt gtctttaaaa gaaggagtat ctacgtaata tgaagaaaaa aatcttagcg
    12301 tcacttttat taagtacagt aatggtttct caagtagctg ttttaacaac tgcgcatgca
    12361 gaaacgactg atgacaaaat tgctgctcaa gataataaaa ttagtaactt aacagcacaa
    12421 caacaagaag cccaaaaaca agttgaccaa attcaggagc aagtatcagc tattcaagct
    12481 gagcagtcta acttgcaagc tgaaaatgat agattacaag cagaatctaa gaaactcgag
    12541 ggtgagatta cagaactttc taaaaacatt gtttctcgta accaatcgtt ggaaaaacaa
    12601 gctcgtagtg ctcaaacaaa tggagccgta actagctata tcaataccat tgtaaactca
    12661 aaatcaatta cagaagctat ttcacgtgtt gctgcaatga gtgaaatcgt atctgcaaac
    12721 aacaaaatgt tagaacaaca aaaggcagat aaaaaagcta tttctgaaaa acaagtagca
    12781 aataatgatg ctatcaatac tgtaattgct aatcaacaaa aattggctga tgatgctcaa
    12841 gcattgacta cgaaacaggc agaactaaaa gctgctgaat taagtcttgc tgctgagaaa
    12901 gcgacagctg aaggggaaaa agcaagtcta ttagagcaaa aagcagcagc tgaggcagag
    12961 gctcgtgcag ctgcggtagc agaagcagct tataaagaaa aacgagctag ccaacaacaa
    13021 tcagtacttg cttcagcaaa cactaactta acagctcaag tgcaagcagt atctgaatct
    13081 gcagcagcac ctgtccgtgc aaaagttcgt ccaacataca gtacaaacgc ttcaagttat
    13141 ccaattggag aatgtacatg gggagtaaaa acattggcac cttgggctgg agactactgg
    13201 ggtaatggag cacagtgggc tacaagtgca gcagcagcag gtttccgtac aggttcaaca
    13261 cctcaagttg gagcaattgc atgttggaat gatggtggat atggtcacgt agcggttgtt
    13321 acagctgttg aatcaacaac acgtatccaa gtatcagaat caaattatgc aggtaatcgt
    13381 acaattggaa atcaccgtgg atggttcaat ccaacaacaa cttctgaagg ttttgttaca
    13441 tatatttatg cagattaatt tacagaggga ctcgaataga gccctctttt caggttttac
    13501 cgtgacaatc cctattaaaa attatatcaa aatagcttga aaatattgga aaagtatggt
    13561 agaatgaaaa ttgtcgtgtg aacgataata ctcattcttg atgaattgtg aagcagttgc
    13621 ccttgggtcg ttttgcgagt tgaagtcaag aagaggaaaa aaacaaaaag gagaaatact
    13681 catggcagta atttcaatga aacaacttct tgaggctggt gtacactttg gtcaccaaac
    13741 tcgtcgctgg aatcctaaga tggctaagta catctttact gaacgtaacg gaatccacgt
    13801 tatcgacttg caacaaactg taaaatacgc tgaccaagca tacgacttca tgcgtgatgc
    13861 agcagctaac gatgcagttg tattgttcgt tggtactaag aaacaagcag ctgatgcagt
    13921 tgctgaagaa gcagtacgtt caggtcaata cttcatcaac caccgttggt tgggtggaac
    13981 tcttacaaac tggggaacaa tccaaaaacg tatcgctcgt ttgaaagaaa ttaaacgtat
    14041 ggaagaagat ggaactttcg aagttcttcc taagaaagaa gttgcacttc ttaacaaaca
    14101 acgtgcgcgt cttgaaaaat tcttgggtgg tatcgaagat atgcctcgta tcccagatgt
    14161 gatgtacgta gttgacccac ataaagagca aatcgctgtt aaagaagcta aaaaattggg
    14221 aatcccagtt gtagcgatgg ttgacaccaa tactgatcca gatgatatcg atgtaatcat
    14281 cccagctaac gatgacgcta tccgtgctgt taaattgatc acagctaaat tggctgacgc
    14341 tattatcgaa ggacgtcaag gtgaggatgc agtagcagtt gaagcagaat ttgcagcttc
    14401 agaaactcaa gcagattcaa ttgaagaaat cgttgaagtt gtagaaggtg acaacgctta
    14461 atttatacaa atagtaatta cctaggaggg cggggcttag cccggctctc ctattttcaa
    14521 aaaatatagg agaattaaaa tggcagaaat tacagctaaa cttgtaaaag agttgcgtga
    14581 aaaatctggt gccggtgtta tggacgctaa aaaagcgctt gtagaaacag acggtgacat
    14641 cgaaaaagcg attgaattgc ttcgtgaaaa aggtatggct aaggcagcta agaaagctga
    14701 ccgtgttgct gcagaaggtt tgactggtgt ttatgttaac ggtaatgttg cagcagttat
    14761 tgaagtaaac gctgaaactg acttcgttgc aaaaaacgct caattcgttg aattggtaaa
    14821 tactacagct aaagtcattg ctgaaggaaa acctgctaac aatgaagaag ctcttgcttt
    14881 gataatgcct tcaggtgaaa ctcttgaagc tgcatacgta tctgcaacag caactatcgg
    14941 agagaaaatc tcattccgtc gctttgcatt gattgaaaaa acagacgcac aacactttgg
    15001 agcataccaa cataacggtg gacgtatcgg tgttatttca gttgttgaag gtggagacga
    15061 agcacttgct aaacaattgt caatgcacat cgcagcgatg aaaccaacag ttctttctta
    15121 caaagaattg gatgagcaat tcgttaaaga tgagttggca caattgaatc acgttatcga
    15181 ccaagacaac gaaagccgtg caatggttaa taaaccagct cttccacact tgaagtatgg
    15241 atcaaaagct caattaactg atgatgttat tgctcaagct gaagctgaca tcaaagctga
    15301 attggctgca gaaggcaaac cagaaaaaat ctgggacaaa attatcccag gtaaaatgga
    15361 tcgcttcatg cttgataata ctaaagttga ccaagcttac acacttcttg cacaagttta
    15421 catcatggat gacagcaaga cagttgaagc ataccttgaa tcagttaacg cttcggtagt
    15481 tgagtttgct cgctttgaag ttggtgaagg tatcgagaaa gctgcaaacg acttcgaagc
    15541 tgaagttgca gctacaatgg cagcagcctt gaataactaa taatagaaag gaagcaattt
    15601 gcttcctttt ttgtataggg cttagatttt tcctgaaatt gtttttcccc cagtctcact
    15661 cgattagaat aattaaaaca aaaagcccta taataagggc taagtgcatt taggagacta
    15721 tacttcaaat tcatagtaag cgcgtcataa caaggtatct atcctaattc cccaacttcc
    15781 agtatactag ataaaaaata attatcggag gaaaggtatt gtactatgat gattcaacta
    15841 agtgatttag gtcaagttca cattgtttgt ggcaagacag atatgaggca gggaatagac
    15901 tcattagcct atgtagttaa aacccacttt gaattggatt ctttctccgg tcaagtcttt
    15961 ctcttttgtg gtggacgtaa agaccgcttt aaagtccttt actgggatgg tcaaggattt
    16021 tggctactat ataaacgctt tgagaacgga aaactgactt ggctaagtac agaaaaggat
    16081 gtcaaagctc tcacatctga acaagtagac tggcttatga aggacttttc tatcactccc
    16141 caaatatagt ggtttgaaac tagaatagta cacctctgct tctaaaacat tgttagaaat
    16201 cgatttgact gtcctgttct tatttcattt tactataaat tcatcagaaa gtcgtgattt
    16261 ctattgaaat gaggactttc tttttatact catctgtttt cacaaagcat tctagtccat
    16321 ttccgattaa cgatggactt tatcacctcc ttctccagtc cttgtataac atcttggagt
    16381 tgattcatga catcttccaa aatttgaaag gctttattct taaatccacg tttacgaatc
    16441 tctttccaca cttgttcaat gggttcatct ctggtgtgta tggaggaata aaggtaaaac
    16501 caatattagt cggaatcttt aaccccaatt attcacccct aat
//
LOCUS       DAVMGZ010000030        16520 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000030 DAVMGZ010000000
VERSION     DAVMGZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.031"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..91)
                     /locus_tag="VYQ93_000834"
                     /pseudo
     CDS             complement(1..91)
                     /locus_tag="VYQ93_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(552..2657)
                     /locus_tag="VYQ93_000835"
     CDS             complement(552..2657)
                     /locus_tag="VYQ93_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV3335553.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AYYRATGQLPGNAESDVQMQQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QEASEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIVSIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(3262..4242)
                     /locus_tag="VYQ93_000836"
     CDS             complement(3262..4242)
                     /locus_tag="VYQ93_000836"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV3335554.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLFTSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGIGLLASLLTLAILYLM"
     gene            complement(4244..6496)
                     /gene="pbpX"
                     /locus_tag="VYQ93_000837"
     CDS             complement(4244..6496)
                     /gene="pbpX"
                     /locus_tag="VYQ93_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV3335555.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIIPGTEQVSQQTVDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGLTKDFVWRDILYQSNYE
                     PGSTMKVMTLAAAIDNNTFPSGEYFNSSELKIADVTIRDWDVNDGLTTGRMMTFLQGF
                     ALSSNVGMSLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     NYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(6500..6817)
                     /gene="ftsL"
                     /locus_tag="VYQ93_000838"
     CDS             complement(6500..6817)
                     /gene="ftsL"
                     /locus_tag="VYQ93_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV3335556.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(6829..7779)
                     /gene="rsmH"
                     /locus_tag="VYQ93_000839"
     CDS             complement(6829..7779)
                     /gene="rsmH"
                     /locus_tag="VYQ93_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV3335557.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQERLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            7945..8139
                     /locus_tag="VYQ93_000840"
     CDS             7945..8139
                     /locus_tag="VYQ93_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3335558.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            8155..8697
                     /locus_tag="VYQ93_000841"
     CDS             8155..8697
                     /locus_tag="VYQ93_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEV3335559.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLRYPGLKAGLFFGLWIFFITPLLDILIGEGQDYFQSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKAKKDQDND"
     gene            8708..8950
                     /locus_tag="VYQ93_000842"
     CDS             8708..8950
                     /locus_tag="VYQ93_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335560.1"
                     /translation="MKKEKKQLRHPGLKAGSIYGIVIFFIIPPIDTLTSDNPNFITSL
                     LNTKHIFKTILGAFFFGVMMHIVDSLRIARAKKDQD"
     gene            8961..9296
                     /locus_tag="VYQ93_000843"
     CDS             8961..9296
                     /locus_tag="VYQ93_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335561.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRISVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(9406..10407)
                     /locus_tag="VYQ93_000844"
     CDS             complement(9406..10407)
                     /locus_tag="VYQ93_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3335562.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(10467..12368)
                     /locus_tag="VYQ93_000845"
                     /pseudo
     CDS             complement(10467..12368)
                     /locus_tag="VYQ93_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
     gene            complement(12390..12683)
                     /locus_tag="VYQ93_000846"
     CDS             complement(12390..12683)
                     /locus_tag="VYQ93_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV3335563.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(12683..13501)
                     /locus_tag="VYQ93_000847"
     CDS             complement(12683..13501)
                     /locus_tag="VYQ93_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV3335564.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(13488..14267)
                     /locus_tag="VYQ93_000848"
     CDS             complement(13488..14267)
                     /locus_tag="VYQ93_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV3335565.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(14282..14773)
                     /locus_tag="VYQ93_000849"
     CDS             complement(14282..14773)
                     /locus_tag="VYQ93_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV3335566.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(14784..15974)
                     /locus_tag="VYQ93_000850"
     CDS             complement(14784..15974)
                     /locus_tag="VYQ93_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEV3335567.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDEFCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(15986..16420)
                     /locus_tag="VYQ93_000851"
     CDS             complement(15986..16420)
                     /locus_tag="VYQ93_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3335568.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
BASE COUNT         4782 a         3771 c         2921 g         5046 t
ORIGIN      
        1 gcaagcgctc atctatgctg gcgactatac ttttcatatt tcccaatgag aaatagttta
       61 tccatcctcg aatgaacaaa ttcagttgct ctcgtgattt acgcatatag gttacgacta
      121 cctttttgaa ctttagagtt tgagaccccc ttatccgcta tatacgcctt actatcacgt
      181 tcctgttcgt aaagccacga tttcgctatc ccttcctctg acgattctta tttccagcgt
      241 cagacttaaa acggtctatc cccagaccgt tttaatccac tacctcacga tagtcaggct
      301 tgggaatcgc tattgggttc accggtagtg ggtgcccacc gaggacttac acctcagata
      361 ttcagatata tagtaaaatg aaataagaac aggacaaatc gttcaggaca gtcaaatcga
      421 tttctaacaa tgttttagaa gtagatgtgt actattctag tttcaaccta ctatacgaca
      481 tgcccgtcgt acctcaaaag gaaccagttt ttttcagctg gctccttctt ttttatcctc
      541 aaaattctat cttaggcttt ctcacgaata accaaaacgc catcttccat atctgcttcc
      601 agatgtttag catctaaatg atccaagtgg aagtctgtca ccttatcacg aatttcttgt
      661 tcaaccactc gacggagagg acgaactccc atgacttcgt cgtaaccttc ttctgtgata
      721 taatctttag ccgcttgact gactaccaag tcaatgtctt tcttagccaa ggtttggtta
      781 acttcagcca acatcaaatc tacaatctta gaaaggtctt ccttagtcaa gtgtgagaat
      841 tcgatgactg cattaaagcg gttgaggaat tctggacgga agaagggttt caaacggtcc
      901 atcaattctg gtttatccgc atcttctgtc aagttggctt catagccaaa tccagcattt
      961 gaggtcgcaa taatgacagt gttcttgaag tttactgtat ttccttgacc atctgtcaaa
     1021 cgaccatcat ctagaacttg gaggagaagg gtaataactt gagggtcagc cttttcaatt
     1081 tcatccaaga gaatgataga gtatggattg cgacgaacac gttctgttaa ggtattgcta
     1141 ttgtcatcat aacccacata gcctgctgtt gtaccaatta gcttagaaac agctgtgcgg
     1201 tcactgtatt cagacatatc caaacggata atcgcatcct gggttccaaa catatcgagt
     1261 gccaattgct tagcaagctc cgtcttacca accccagtag accctacaaa gaggaagctg
     1321 ccgattgggc gatttccttc atcaaaacca gcacggttac gacggatagc acgagctaca
     1381 gcttctacgg ccttatcttg accaatcacc ttgtcttgca agcgatgagc catatctttc
     1441 aaacgttcga tatctgaagc tcccatttgc gatactggga tacctgtcat tcgttccaca
     1501 gattcagcca catcgttgac acttgcagtc actttcatat cttctgtgtg gttttcgatt
     1561 ttcttttcca attctgcaat gcgtgtttta tagtttagag ctgcttcaaa atcttctgct
     1621 tcaactgctt tttcttgctt gtctttttcc gtttcgattt ctcgttcaac agcatgcaca
     1681 tctgttactg gatgttgagc cgccaagtga gcagctgtta catcgacaag gtcaatagcc
     1741 ttatctggca agctacgttg aggaatgtat tgaacagaat aatccaccgc tgctttcaag
     1801 acttcgtctg gcaagatgac attgtggtgt tgttgataga ggtcacgaat tccttgaaga
     1861 attttaaaag tattctctgc cgaaggagca ttgaccttca cttcgttgaa acgacgagca
     1921 agagcagcat tcttcaagat ggtgttacgg tattcgtctt gagttgttgc cccaatcact
     1981 gtcaattctc cacgagagag agctggcttg agaatatccg caagcccttt agaaccactg
     2041 tctccaccag tgctaccagc accaagaatt tggtgaattt catcaaagaa gaggataata
     2101 ttccctgctt ctttcacttc attgactaag ttttggacat tttcttcaaa gctaccacgg
     2161 tattgagtac cagcctcaag acctgagata tcaatggaaa caatttcctt gttcttaata
     2221 gcagctggga catcgccgtt cacaatcgct tgcgctaggc cttcgacaac ggctgtctta
     2281 ccaacacctg cgtctccgac taaaacagga ttgttcttgg tacggcgtga gaggatttca
     2341 gatgcttctt gaatttcctt gtttcgtccg ataacaggat ccagcttgcc ctcacgcgct
     2401 tctgctgtca agtttcgacc cagtttagca aggacaccat cttgtttcat acctgaagcc
     2461 tgttgttgca tttgcacatc agattctgca tttcctggca attgaccagt tgcacgatag
     2521 taagcaaatt cctcaggtgt cacttcacgt ccattaatca agtaacgtcg attttcagaa
     2581 ctgtatcctc gcataccacc catcaattgg ttaaataaat catccatgtt gttaaaatta
     2641 ttaaaattgt tgttcatatt ctttacctct ttttgttatt tattattact gatattgact
     2701 atctttgacc ttttctttaa aaaatttaga ctagttaaac cgctacccta tgtactattt
     2761 ctggtttttc tttttcaagc aggagtagac tatctttact tctgaagatt tctagatgaa
     2821 acatagaccc cacctctttc tattttactt taaataagtg ttctagtaag gctttcattt
     2881 accttacgtt cataatatac tacattagtc agaaaaggtc aagcattttg actttaactg
     2941 accaatattt ttttacactc ttcgaaaacc tcttcaaacc acatcagctt cgccttgtct
     3001 acaacctcaa aaccgtgttt tgagctgact tcgtcagttc tatcaaccat ctcaaaactg
     3061 tatagtaaaa tgaaataaga acaagacaaa tcgatcagga cagtcaaatc gatttctaac
     3121 aatgttttag aagttacggt gtactattct agtttcaatc tactatattt tgagcagcct
     3181 aaagttagct ttctagtttg ttctttgatt ttcattgtgt atttaaacac aaaaacaccc
     3241 tgaaacatca gggtgccatt cttacatcaa atataaaatt gctagggtca ggagacttgc
     3301 tagaagccca attccccaaa agaagaagtc aaccttccac tcgctccaag gatttccttt
     3361 accagacaat cccccaagct caaaatggtg atgtacaggc gtcatacgga aaatacgttt
     3421 accgcctgtc agtttgaaat aactgacttg catcatgacc gaagttgttt caaaaacata
     3481 cacaattccg ataatcaaga gagtccattc ttggtggagg gccatagaga tagctgccag
     3541 cattccacct agggccaaac ttcccacatc tcccataaag accttggcag gcttatggtt
     3601 aaagatgaag aaaccgagca aaccaccaat catggcaaga atcactagaa gaatatccat
     3661 ctgaccttgc acataggcaa taactccata ggcagacaaa ctaatcacaa cggaaatact
     3721 agctaaaccg tcaacaccgt ctgtcaagtt tactgcgttt gaaaaaccga ctagccagaa
     3781 aagagcgaag acaatataga aaatccctag atgcacttga taaccaaaga cagaaagcat
     3841 atcgccccca cgctcataga aaagatagaa gataactcca cctagaagct gaagagctaa
     3901 tttttgctta ggattaagcc cctcattgat tttacgaaag accttgagaa agtcatctaa
     3961 aaatccgacc aagccataca agaccaagat gaacaaaatc attcccacat tattgctgaa
     4021 ttggctacta aatagggcga aaaagaaagc aaccaaaaca gaagtaaaca agaaaaccaa
     4081 acctcccatt gtaggagtcc cagcttttgc ctgatgctgt ttgacatcct catgcatctg
     4141 ctggcctgta atttgcgcct ttctataaaa ttggataaag gccggaattc ctactaaagt
     4201 tagtaaaaat gtcacaattc cagcactgat agaaataaac atattagtct cctaaagtta
     4261 atgtaatttt tttaatgtcc ttgatagctg tgttagcacg aacatcttgc ttctgcacag
     4321 tagagcccga accttgaaat tcaagttcta tattgagcca cttagcaagg gtctcagcag
     4381 tctcctttgt ccaaccatac atatctggaa cctcctctgc tttatcagat aagataagga
     4441 cttgctgatt cggggcaaga ttcttccctt cttcagcaga actgttttta atcttcgttc
     4501 ctgttcccac aacgatgggt tgtacaagat tgcgacgcaa ttcttctgct aaatcaccag
     4561 gtgaaatatc cttgacacta ggcataggat aaggactttg ttgacttact tgctccaagg
     4621 ctttagctgt tgtttgaaga ttgagagagt ctttcatagc tgaagcccgc tccaagatag
     4681 gattggcaaa ttctcccaac tgaatacctg aataatgttc aggttgttgg accgtcacat
     4741 acaagataaa atcaggattt tcagccggac tcatcgatac agccgagaaa atatagttgg
     4801 ttaacccgac tagataacca ccatttttct cgtcagcaat ctgagccgta ccagacttga
     4861 gggctacatt ttgcccagga acagttacag ttggcttgcc tgtgctgtgg ttatacatgg
     4921 ttccataaac cggatccgtc cctaccaaaa ccatgttagt ccgagttaga ctagctgcat
     4981 ctttagaaac aggatttccc acaatttctt tttgagattt ccgagcagtt tgatcatttg
     5041 gatcataaat ggcactaata aatttaggct ccagcatgac accgtcatta gcaatagctg
     5101 taaaggcacg aatcatttgc gtctgggtca ctgaaatccc ttgcccaaat gagctttgcg
     5161 caatgttaac tatattatcc gcaggaagct gaccagcata ctcatctgtc aaaccgaaac
     5221 gggtcggaac accaaattta aagcgattaa gataatcaag ccaggtagca tctcccattt
     5281 tttgttcaag tagactcatt ccaacattac tggagagagc gaaaccttgt aagaaagtca
     5341 tcatcctacc agtagtcaaa ccatcattaa catcccaatc tcgaatcgtc acatccgcta
     5401 tttttaattc actgctattg aagtattctc cacttgggaa ggtattatta tcaatagcag
     5461 cagcgagcgt catgaccttc atggttgacc ctggctcata gttactttga tagaggatat
     5521 cacgccaaac aaaatcctta gtcagccctt ccttagtatc agcatcgaag gttggtctct
     5581 gcgtcgttgc aagaatctcc cccgttttag cactgaccaa agttgctgtc atgtactttc
     5641 cttttacctt ctcttgaaaa gcatccatct gggtttccat aaaggactgg agggggctgg
     5701 aaatggttgt ataaacatcc ttaccgtcca ccgtttgttg ggaaacttgt tctgttccgg
     5761 gtataatatt gcccagacga tccttttcat aggtaataat gccgtctgtc cctgcaagaa
     5821 tactgttcaa ggaactctcc attccagagg ttcccagcaa actcttactt ccatcttcat
     5881 tttcatgtag ctgagctagt ccgataaaac tagaagcaaa ttgtccgttt gggtaactac
     5941 gattgggact ggttgtaaaa tcaatcccct tgacctctgc agtttccaac tcttttttga
     6001 tagacatcat attggcatag gtaatcccat tcccctttgc tccaaaggaa acttgtttga
     6061 gattaggttg cgagagttgc tctcttacat aggattcttc catgtccaga tacttatgaa
     6121 agacctctgc aaccttgtta aattgtgttt tttctacgta aagaatctta cccgttgctg
     6181 acttatagtt ctcatcaatg accgcataga cattatagga ggttgcatcc tcagcaatcg
     6241 ggactccatt tcggtcataa atagtcccac gtttggcagg aactgtacgg gtggtttgat
     6301 gaaccttctt agcttccttc gctaaatctg ttccaaagcg agtgcctgtc ccaataatga
     6361 ccgcaaaatt gactaaaaaa atggcaaaaa caaagacaga taataaactc agactttttc
     6421 caactctgcg tctgttttca gccggcgatt tccgattttt ggtcgcataa cggattactc
     6481 tttttgtcca cttcatatct tactccgcta ttctaatatt ttcattgttt aattgcaaat
     6541 cgtgtgaatt ggcaatttct ttcaaacgtt ctgcacgtaa tagttcattg acctcttgct
     6601 tggcatcgtc caattcggtt ttcttttcct ctatctgcgc attgattttt gtcaaatcat
     6661 tctgcacttg caagagcttg gtctgcataa aaataatact aatggctaca ataagagtgg
     6721 ttacagcaat ggaaaagtaa aaagcttttt ccacacgcga aaaccgttta agttgcatct
     6781 gtagtatttg acctgttttt tccatttttt ctgccatctt tttccctctt acttgtgaat
     6841 ttttctggcc acgcgcaact tggctgagtg cgagcggtta ttggcttcta actcttccgc
     6901 acttggcaag attggcttac gggacaccaa ttccatcttg ggcttgagat catctgggat
     6961 gaaaggcaag ccttttggaa cttcaactgt tgaagcttcc ttgaacaatt gcttggtcaa
     7021 gcggtcttct aaggaatgaa aggtaatcac tgaaattcta ccatccagag ccaacatatc
     7081 catagcctgc tggatggact catctgccgc ccccagttca tcattgactt caattcgaat
     7141 agcctggaaa atctgcttag caggatgccc cttcttcttg agttccttgg caggtttgac
     7201 caacttgata atctctgcta actcagtcgt tgtctcaatc ggcttcactt cacgcgcttg
     7261 ctcaatctta cgcgcaatct gtttagagaa tttgtcctct ccatacttga agaaaatacg
     7321 aaccaagtca tgatagtcat aattgttcac cacttcatag gctgtcagac tagcatcctg
     7381 attcatccgc atgtccagtg gcgcatcctt tttataagaa aaaccacgct cacgctggtc
     7441 taattgagga ctagacactc ccaagtcata acaaattcca tcaatttcct gaacaccagc
     7501 ttcgcgcaaa cgtgcctgta aatgacggaa gttatccttg ataaaggtca ccattccctt
     7561 ctcaatgtag ggtgccaagc gttcctgcgc attgtcaatg gcattctggt cctggtcaaa
     7621 ggcatagaga tggccttttt cacttaattt acttaataaa tattcgctat ggcctgctcc
     7681 acccaaagtc gcatcaacgt agataccgtc gggctttacg tcaagcatat caatcgtttc
     7741 gtggagtaag accgttacat gatgaaattc ttttgtcata tcttatctat tttaccacaa
     7801 atccgaccag cttgcactag tcagacaaat cagacaagag cgctactatt tcttcaaaaa
     7861 atatgtcaaa aatacttgac actttatgtt taaaagaata taatatagtc aaatatatac
     7921 gacacaaatc agaaaggaga ccagatgaat cgtgtgaaag aatttcgcaa ggaattgggt
     7981 atttcccagc tcgaactcgc caaggatatc ggtgtctcga gacagaccat caatatgatt
     8041 gaaaacgaca agtacaatcc aaccctggaa ctctgtctca atctcgcccg cagcctccaa
     8101 actgacctca acagtctctt ttgggaggaa gatttttaaa aaaggagcaa actcatgaaa
     8161 aaagaaacct tcactgaaaa actgatcaaa cgcacatacg gtatttctgg tccccttgac
     8221 gaatacaaac ggcgtgaggc cgatcgtatc ggcaaccaag tctttatcgt cctcttttat
     8281 ctgatgattt tcggaaatct tattccactc cttctggcct ataaatatcc tcaagaagtg
     8341 gctctaatct atcctcctct gattttagtg attgccctca tcgctgctgg ctatgtcacc
     8401 taccaaatga aaaaaacagg catcacagtc attgaaccag atatgctgaa tgagaaagaa
     8461 agcaagcaac ttcgctaccc aggtctgaaa gcaggtttgt tctttggtct atggatattt
     8521 tttataactc ctcttctcga tatactcata ggtgaaggtc aggactattt tcagtctctt
     8581 ctcactataa gaaatggtgt atcaagcatt ctcggttcta tcttcttcgg ggcaagcata
     8641 cagttcctca tctcccgtcg cattgcaaaa gccaagaaag atcaggataa tgattaggag
     8701 gaaactcatg aaaaaagaaa agaaacagtt acgtcatcct ggtctgaaag caggttcgat
     8761 ttatggaata gtgatatttt ttataattcc acccattgat accctaacaa gtgataatcc
     8821 aaattttatc acttctcttc taaatacaaa acatattttt aaaactatac tgggagcttt
     8881 ctttttcgga gtgatgatgc atattgtcga ctcacttcgt attgcaagag ctaaaaaaga
     8941 ccaggattag gaggtgcctt atgaaatcac tagctagact actgatcatt catgttttta
     9001 tcagtatttt ccttttcttc gcccttattt caggagctgt ttctcataca gttttactac
     9061 tcctactcct ctttcttcct gcgctcaata aaggacttga gaaaatacaa tcaaaacgga
     9121 tatctgtcct caacgcagcc ctcttctttc tcctcatatc ctttccacaa cttttaacca
     9181 accctgtcca atggaaattt tcaatattcc tagtcgtaac catcatttca agtttggcct
     9241 acttctataa cttttatcaa gtagttaaag aagtagatca aaaacagttg atttaggagc
     9301 ttgctcccat aatttctcat ttcataggca aataatccca cctagcttgt tctaggtggg
     9361 attattttat ttcttaacag agatcgcaag tcattttcct tcattttaga aagtcgactc
     9421 tttccaattc acactacaat ccaagacttg ttgcctttct tcttgattgc ggccttcaat
     9481 ctggtcaatc aaaatctttg tagcctgttg tccttcctca aaggagggct gaaccagcgt
     9541 cgaaacactt ggagaagaaa agcaagtcca ctccgtattg tcaaaaccaa tcaacccaac
     9601 ttgtggcaag ttataattca actctttgat aacggtaaag actagaggta gggcccaaca
     9661 gttagggata aataccagag ttttttcatc gggatcgatt tctttttgta aaaattcctt
     9721 aatttgttcc aaattcgtat gcttatcttc aatggttaga ctggcgtgac gcatattagc
     9781 atctgttaaa gcatccacaa aaccacttgc ccgctcaatc cgagtactca aacgactcgt
     9841 atccgctgta atcaagagaa aatgttcata acctttttcg atacaggact gggtcatgtc
     9901 ataaacggca tcatagttat tggttttaac ccagctagtc cggtgttcat agagctgact
     9961 atcaaaaaag accattttct tctttttctc atcgatgata cgagaatatt ttcggaaatt
    10021 agaggtcggc tgaataataa agccgtctac tcccaagaga agcatgcttt caatataccg
    10081 gtcctcactc tcttggctgt aattactatt tcctatcatt acctggtagc cattctggct
    10141 ggcgatatcc tcaattccct taacaatttg gtttgagaaa ctgttggtaa tatcaccgat
    10201 caaaacaccg attaattttg ttcgtttgga gtttaagcta cgcgcaacaa tgctcggttt
    10261 gtaatttgtt tcatgaataa ctttttcaat cttttcacgt gtctcttggg acattttttc
    10321 atatttccca tttaggtaaa atgacacggt tgttttcgag gtctgagcca tttccgcaat
    10381 gtcttttatg gtcagtttct tctccaatct aaaacacctc ctactaatac ttattataac
    10441 atattcacta ttcaaaatgc ctatttctag cactgcaagc gaatcattct ctcatttatt
    10501 ttatcatata ctagacattt tccacgcatt tttgttccgt caacgaggca caatttttca
    10561 cctacaccaa tatcctcgct taataacaga atcagcttgt ctgtttcatc atttttcact
    10621 tcaaaagcca gagcattttc cacttcacga tcatctgtct ggtagacaga atgacggatg
    10681 atttcaaaac tttcatgcgc aatgatggta taatccagca tctgattctc aaagaattgg
    10741 cgcttggtga gtttgctact tctttcaagc tcattgtatt taggagaaat gatggtatct
    10801 tccaaatcaa aatcaacttc actccatagt ctcaactgat tgattttccc atcttgatag
    10861 gtcacatcct tgtcaaggat aaactgagtc aacgcctcat gctgaccttg acacctgatg
    10921 tcatccacca agagccagac atcctctacc aacatgagga tttttcttct gtgaagataa
    10981 ggcaaatcag gttctgctga ccaataagcc ccctcaatat aatgcactcc ctccctttct
    11041 ttatggtgac aaaacaggga gtgaggatag tattcatatt cccaggatcc cgtgattctt
    11101 tccggagctt tcccatctac aatgcaggtc gaatgactcc aagcactctt taagagataa
    11161 cgttcttata tctcccgata agaataacgc ccagcatcta tgaaaatagg ttggccttga
    11221 tactgtaagc aaaaactatt ctcgtcacta tggctatggg cacttcctag cggaccattt
    11281 ttgaaaaata gataacgatg ttcatcctta atgcagacat gtccagagtc ttcaaagatc
    11341 atggacttag gctgccaagc tctcttttca aattcctgca gtcgcttgac cttttctcgc
    11401 cccaggaaca agaggctaag caaatcgact ttaacatcca gaccgttaag aaggtcttcc
    11461 ttgttcaaaa ccacagcaga caggctcaaa atttctgtcg tttctgtaga atcgctatca
    11521 ccaaaagcca aagttcgtcc atctaagcct gtcatcattt gaatataggt cgccatcttt
    11581 tccagcaact cttggtaact atcttgcaag tctggaagca agagacacaa atccagcaag
    11641 gctttataaa cctctacatg atagagaatc gactgttcaa actggcttcc atctcctaaa
    11701 atctgtgtct caatttgctg tttcaactcc tctgaagcaa aatggtaagc ttcttctagg
    11761 tccatcttat ctgaaaagaa atgatagata gcaagcatcg gaattgtttg taaaatcccc
    11821 cagttactaa gggtgtactt ggcgcgatag tagcttttca taaagtcaat ctgcttttct
    11881 agactgacca aaattttctc tagttctttc tcctctagca agtcaaattt caagagaagc
    11941 aagagtagtt tcaaccaagt aaaggaacga atacccgtat ccaaggttct agtcatcaag
    12001 gattgaggag aaaattctct cacctgctca atccaatcaa atagaaagaa cttgcacttt
    12061 tgaatatagt ccttatctcc ttctaccaca taccctatca taaactgcaa gagatattct
    12121 tgtcgattga gcatataaga ccattctgga tcatcttcaa atacttgatc ccataccatc
    12181 ggctggattt gatggatttt tgaacaaggc tccatatccc aaggactatc aaacataaaa
    12241 cgattgtcca tcaagcgttc aagggaactc ttgactttct catagtcttt tgaacagtgg
    12301 gacaagatat aatcacgaca ttggtttcca tcgattcttt caaaaaattg tcttctttct
    12361 tctttcatta tctattacca gaaaaagaac tacttaaaaa gcagttcttt tgtctttccc
    12421 attacacttt ccttttctac atagatgacc acaccttttg caatctgcaa ggagaccaag
    12481 tcatctttga tagaaatgat ttttccatga attccagaca ataacaacac ttcatcacca
    12541 gatgttaaag aagctaaata ctcttgtcgt tgctccatct gtttgcgaag caacttttgc
    12601 tgacgaatag aatgaaagct tgacagtaaa aggggactca ctgccaagac aatcactatt
    12661 ccataaaaca atgttgtatc cattaagcta taatcttaag ccagcttccg ataattccga
    12721 tgataactgt taaaataacg agtttatatg ttgtccattt cttttctttg atcaagtagt
    12781 aaactaaaag tgtaaatagg gctggtagaa gagctggagc aaccttatca agcattccct
    12841 gaatacttac gatactttgt ttagcgtctg ctttaacttc ccctgcagca aaggtaatcg
    12901 gcaccataat cttaacagat gtcgctgcca aaccagcaat tacggttaca ccgataatat
    12961 tggcaatacg agaaatcgtt gccatctgtt cgcttagttt atcaatcaca gttgttccta
    13021 gtttgtatcc atacagacca gttgacaatt taatcgctgt taaaatcgta ttcatcgcaa
    13081 ggaagaacaa gattggaccg acaaccaagc cttcttgagc aaacgaagct gcgatggttg
    13141 agaacaatgg agctaaacag aattgagaaa gagaatcccc aatacctgcc aatggtccca
    13201 tcaaggccat cttgatgcta cgtgtttctt ttgccggacg gccattttcc aacattacaa
    13261 gatgcaagct ggtaataaaa ggcaggaagt gtgggttggt attatagaat tcacagtttt
    13321 cttccaaggc ttggtagaaa ccttcctgat cctctccata gtgttttttc aaagcaggat
    13381 acatcacatt ggcatatccc aacccttgat agttactata gttaaatcca ttttgacaaa
    13441 agaatgcccg caaagacgtt ttaagataat cacgttttgt taatttgtta gatccagtca
    13501 tcgtgtgctt cctcctctac cacatgatcc gctgtttttg gcttgttata aaattcaatc
    13561 aaagcaaaga tagtacctac aattgcaata ccaattgttg ggatgtttag ataagctgca
    13621 caaacatatc ccaacaagac aaagggaatc aactctttct tagccatcac tgacaagatc
    13681 atcgcaaaac cgatagctgg gagcatttta ccagcaactg tcaaacctgt aagtaatacc
    13741 ggtggaatgt agtctacgag tttcaacaag gtatccattg aaagggcacc caagcaaccc
    13801 aaggtaaatc caataaaggc aaacaaccaa attgttgcat ttagagtgaa cttaaatttc
    13861 ttcaaattat ggtttttcaa gtgcttcatc gccgtttcag gcgcaccagc acgtacagtg
    13921 taggcgaaag tttgtaagaa ttgaatcgcc acagcaatcg gagtagagag ggcaagagcc
    13981 gcttctggac tgactttacc agcactagtg atagccatca aggtaccaaa gataccaggt
    14041 ccgattgggt ttggtggaac agtacctcca gcaccaacac cgaatcccat ataagccaat
    14101 tcaccaatag ctcccattgc aagagcagta ggcagatcac ctagaataat tccgacacca
    14161 aatgacagaa caatacatct attggtgtaa attcctaaca gcattccact aaaacagaaa
    14221 gctgtccata atccaattaa aatcgcttga aatacattaa tcgacataac gatctcctct
    14281 tttaaatata gtccataatg ttgacttgaa cagctccatc atttcctgtt ggagttgttt
    14341 tcgtattaaa tgttacttga tgagtttggc ttaattcctt gagggctgcc ttgtcctctt
    14401 cacccaggaa gatggagcgt gtcacttgct ctttaccagg ggcattgtga atgttcccaa
    14461 tattgatttc tttgataggg acaccacctt ctaccaaggt taaagcgtcc tttacatcct
    14521 ttacaacgat aaagatcgtt tgagcaggat tagccttgtg aatgatatca atcacctttt
    14581 gcaaagggaa gaaacgcatg gcaactgagt ctggcacaac tgttttcatc agagtttgtt
    14641 gcatcttgtc cgtgcttact tcgtcattgg caacaatgac cgtattacaa cctaggtatt
    14701 ttacccaaag ttgtccttgt ccatgaatca accgttcatc gatacgggtc ataataatat
    14761 ttggcattgt catatttctc ctcctaccaa tataggttcc agtctttgta gaaacggata
    14821 agggcttcta ggtaatagta gtcaccccag atattgcctt catctactcc tttacctgaa
    14881 tgccatgagt acacaccgtg gaggagactt gtcccaccag gggtaaattg atcatttgca
    14941 taatgttcga tcaaggaacg aagcatggca tgcatagcat gtttataaat atctttgtca
    15001 gcatccacct ctgggagatg ttttagcatt tcatgaatcc cacagacggc gatagctgtt
    15061 gctgaagaat ctcgtgattg atcactacca tcattaaaaa tcaaatccca ataggacaca
    15121 tgatcttttg gcagacgatt caagaaataa ttggtcacac ccttaaacaa gtcaaagcag
    15181 aactcgtctt ttaagtgacg ataagtcaaa ggaataccat agactcccca tgattgacca
    15241 cgtgcccagc atgaatcatc actataccct tgtctcgtta cacctttaaa gggttgacct
    15301 gtctcaggat caaaatagaa ggtgtggaag gacgaagcgt catcacggat tacattatta
    15361 gctgaagcat agaaatggct ttctgcaata tcgtagtatt tttgatcgcc tgtttcttga
    15421 taagcaaaga ataagagttg gatattgagc aagcagtcga taatcaaacg gtaatgctct
    15481 ttcttgccca agtctcccca agcttgaata aaaccacctt tttcttgata gcgttcaatc
    15541 aacttatctg cagctttcaa ggttgcttct ctagcctctc catctccatt tatcttatat
    15601 tcagccatac aagacggtgt gtacaagaag ccgagatcat ggtgatccaa ttctactctc
    15661 ttattgacac gatccaggaa agaaagaaca tttttatgag cgatgttttt aaatgcatcc
    15721 tgttgactgt attcataagc caaccacagt tctcctgtcc agaaaccatt ggtccattcc
    15781 gtgttatcca tgattggata gacattatca aaggtagctg gcgtcgggaa atcttccttg
    15841 aaatagtcaa ggttgagttc taactgccga ataaccttat cgattgcctg gccgacttct
    15901 tctttcgtca gaagtggtac ttctaagaag cgctcaggcg attttatttt ttcaatcgta
    15961 acctttttta tcattctttt accctttaaa tacccgattc gaaatcttct tcctcttcct
    16021 ctgcatccgt tgaaaacaat tctttaccat ttacgactcc gccctgggcc gccactactg
    16081 atttattaac aacctcatca aagctatgag ccattctagc aaagactgct tccattaaca
    16141 tggctaagtt caaacccgag agaacattca ttgttttggc tggattttct cccattatgg
    16201 tagaagaaac cttgaatggc gatcctccca agagatcact taggattaaa acttcttctt
    16261 catttgaaat tgcaagtaag attttttgct tgagttcatc tgctgacatt ccttccacaa
    16321 agtcaattgc ctccacattt tcttgattgc ctgcaatcaa ttgtaaagaa ctataaatcc
    16381 ctgtagcaaa atgtccatgc cctacaagta caattttcat cttttctcct ttcttgttta
    16441 ggtttaccgg tttactttat attccccatt gtaatccttt atttttatct tgtcaatagt
    16501 ttttttaaaa aaatattagc
//
LOCUS       DAVMGZ010000031        16383 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000031 DAVMGZ010000000
VERSION     DAVMGZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16383
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.032"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            90..1868
                     /locus_tag="VYQ93_000852"
     CDS             90..1868
                     /locus_tag="VYQ93_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV3335569.1"
                     /translation="MSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIY
                     WNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIG
                     RSAMVTKLLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYP
                     ELEPSKLGTMLFEYSKLFMENKEDNSTMDSSNNFSHQLTQSLLKSPNLILRGAPGTGK
                     TYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKD
                     FCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSG
                     VPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKF
                     VFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIG
                     TMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIEN
                     VQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAF
                     ELTNNEQKDQTVADDNEGDENDDADY"
     gene            1855..3171
                     /locus_tag="VYQ93_000853"
     CDS             1855..3171
                     /locus_tag="VYQ93_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV3335570.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEIGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            3346..4332
                     /locus_tag="VYQ93_000854"
     CDS             3346..4332
                     /locus_tag="VYQ93_000854"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV3335571.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4396..4467
                     /locus_tag="VYQ93_000855"
     tRNA            4396..4467
                     /locus_tag="VYQ93_000855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4429..4431,aa:Arg,seq:ccg)
     gene            4650..5348
                     /locus_tag="VYQ93_000856"
     CDS             4650..5348
                     /locus_tag="VYQ93_000856"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV3335572.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5339..8878
                     /gene="smc"
                     /locus_tag="VYQ93_000857"
     CDS             5339..8878
                     /gene="smc"
                     /locus_tag="VYQ93_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV3335573.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALDIALGASSQHIIVEDENAATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEVALKTLQDEMARLTESLEAIKSQGEQARIQEQGLFLAYQQTSQQV
                     EELETLWKLQEEEKDRLSEGDWQADKENCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVTDIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8875..9669
                     /locus_tag="VYQ93_000858"
     CDS             8875..9669
                     /locus_tag="VYQ93_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3335574.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLGKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            9669..10487
                     /locus_tag="VYQ93_000859"
     CDS             9669..10487
                     /locus_tag="VYQ93_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3335575.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            10491..11780
                     /gene="ftsY"
                     /locus_tag="VYQ93_000860"
     CDS             10491..11780
                     /gene="ftsY"
                     /locus_tag="VYQ93_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV3335576.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVDEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDSEPVVGVSQEEVEEFPHSEELPEEEKLDHE
                     GTIEENNSEVLEAERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVLEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11826..13313)
                     /locus_tag="VYQ93_000861"
     CDS             complement(11826..13313)
                     /locus_tag="VYQ93_000861"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV3335577.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTAATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(13462..14202)
                     /locus_tag="VYQ93_000862"
     CDS             complement(13462..14202)
                     /locus_tag="VYQ93_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3335578.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14202..16367)
                     /locus_tag="VYQ93_000863"
     CDS             complement(14202..16367)
                     /locus_tag="VYQ93_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV3335579.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
BASE COUNT         5104 a         2927 c         3628 g         4724 t
ORIGIN      
        1 tattaaataa ggcaactgtt ggtgaaaagt ttaatgataa attaaaagaa gagtttctcc
       61 aggaatggcc tttggatagg attttaacaa tgtcaatcga tgaatatgta ataggaaagg
      121 gacagcaaaa taagtcttta tgctacgctc ttgagaaggg aaaatacaaa aatctatttc
      181 ttggaatttc tggtggctca gcttcaaaat ttggtattta ttggaataaa aaaacaaaca
      241 aatataaaga tcaagctaat aatgagattt cagagttgga tcagcgattt tcaaaattaa
      301 aatcagattt gtatgaaatt atcaaagaag gtattcgttt taactttgaa aatcctattt
      361 ttgatatgaa aagatcaaca aatgaattta ttggtcgttc tgctatggtg acaaaattac
      421 tttgtatcta tactgaggga gatcctttct ttggtgtaaa tattaatagt cagaaagaat
      481 tttggaacca ctttgtttct cagacaaatc aaggtggacc ttatctgcaa aatcataaaa
      541 taattgaact agtgtccaaa acttatcctg agttggagcc atcgaaatta ggaactatgc
      601 tttttgagta ttctaagctt tttatggaaa ataaggaaga caatagtaca atggattcat
      661 caaacaattt tagtcatcaa ttaactcaat ctctattaaa gtctccaaac cttatccttc
      721 gtggtgctcc tggcacagga aagacttatc ttgctaaaga aattgccaaa gaattaacgg
      781 atggcaacga agaccaaatc ggatttgtac aatttcaccc atcctatgat tatacggatt
      841 ttgtagaagg tttaagacca gtatcaaatg gggatggagc tattgagttt aggctacagg
      901 acggtatttt taaagatttt tgtcagaaag caaaagaaac ccaattgatt ggaggacaag
      961 ataattttga tgaggcttgg gattcttact tagaatatat aaatgttgct gaagaaaaag
     1021 aatatataac aaaaacatct tacttatctg ttaatagtag acaaaatttg tcagtaaatt
     1081 atgatagtgg tgttccagga tggtcactac ctagcaaata tgtttacgag ttgtataaag
     1141 ataaaaatta taataagcaa gaatactaca aaagtggtgg aaaaactgtc ctagaaacat
     1201 tgagaaagag atttggtttg aaagactatg tttccccaac agaaattgat actgataaga
     1261 aattcgtctt catcatcgat gagatcaatc gtggggagat ttctaagatt tttggcgaac
     1321 tctttttttc tatcgaccct ggctatcgtg gtgaaaaagg aagtgtttct acccaatatg
     1381 caaatctaca cgaaactgat gaaaagttct atatccccga aaatgtttac atcatcggaa
     1441 ctatgaatga tattgatcgt tcagtggata cctttgattt tgctatgcgt cgtcgttttc
     1501 gttttgttga agttactgcc gagggtcaag ttggcatgtt ggataaagag ttgaatatcc
     1561 atgcagaaga agcaaaaatt cgtctaagaa acttgaacgc tgctatcgaa aatgttcagg
     1621 aattaaacag tcattatcat attggaccaa gttattttct taagttgaag gatgtagatt
     1681 ttgactatga attactctgg tctgattata ttaagcctct cctagaagac tacttgcgag
     1741 gttcttatga tgaggttgaa actttggaaa ctttgaaaaa agcatttgaa ctgacaaata
     1801 atgagcaaaa agatcagaca gtagctgatg acaatgaagg tgatgaaaac gatgatgcgg
     1861 attactgata atcaacacaa gattattaaa gaaaaatttg ttgaagaata tcctaaacta
     1921 agcaatcttc ttttagacag aaccttggaa agtctatccc aagatgaacg tattttcatt
     1981 tttccaaatg atttgactca tactcctgat ttggataagg accaaaagat ttttgaaaca
     2041 gtcaatcaga aaatcaagac agggaacgtg attggttttc ttggatatgg tcaggaaaga
     2101 ttaacgattt cctcacgatt ttctgatgag agtaatgacc attttttgca ttatctctta
     2161 aacaaggttc ttcatatcaa tctcactagt ttagatgttg ctttgtctcg tgaagagagg
     2221 ctttatcaac ttttgatgta tctcttcccc aagtatctac aagctgctat tcgaaaaggt
     2281 ctttataagg aatatcatcg attttctcat aacgacagtc atgttaaggg agtgattgat
     2341 gtaagaagcc atctcaagaa aaatcttcct ttcacgggaa atattgccta cgcaacgaga
     2401 gagttcacct atgataatcc cctcatgcag ttggtccgtc acactattga atacattaag
     2461 aatcagaaaa gcattggtca aggggtacta gataatctct caactagtcg tgaaaacgta
     2521 tctgaaatcg tgcgtgtaac gccctcttat aaactagctg atcgtgctaa gattactcgg
     2581 agaaatcaat ctaaacctat acgtcatgca tactttcacg agtacagaaa cttacaagaa
     2641 ctttgtctga tgatcctaaa ccaagaaaag cacggtttag ggtatcaaga tcaaaaaatc
     2701 tatggtattc tctttgatgt tgcctggctt tgggaagagt atgtttacac cttgttgcca
     2761 aaaggttttg tacatcccag aaataaggat aagacggatg gaatttcagt attttctgtt
     2821 gggaaacgaa aagtatatcc agatttttat gacagagaac gaaagattgt tctagatgca
     2881 aaatataaaa aactggaatt gactgaaaaa ggaatcaacc gtgaggactt attccagctg
     2941 atttcctatt cttatatttt aaaagctgag aaggctggac ttgtttttcc gagcaaggac
     3001 aaggtcattg ataatgagat aggaaaccta gcaggttatg gagctttgtt gaagaaatgg
     3061 tctatccaaa ttcctcagaa gtcttcatct tatagtgcat tttgtaaaat gatggaaaat
     3121 tcagaagaaa attttaaagc gattattgat gaagaagtgg ggagaaaata atttcccctc
     3181 tttcttttat ttttaacaca agcaaaatcc gaacaatata ttttaattca taaaataatt
     3241 tgacaaaaac tgtactttgg tttataataa attaaggaaa cgtcggaaaa ggcgtagtga
     3301 tgcgagagca taggtaggtc attacaaaag aaacgagaca tcgatatgtt aaatgaattt
     3361 ccaatttttg attacgaaga tattcaattg attccaaata aatgtgtcat taaaagccgt
     3421 gcagaagcgg atacaagtgt cactttagga aatcacacct ttaaactacc tgttgtgcca
     3481 gcgaatatgc agacgatttt ggatgaaaat gtagcagagc aactggctaa aggtggttac
     3541 ttctacatta tgcaccgttt tgatgaggca ggacgcattc cttttattaa acgaatgcac
     3601 aatcaagggc tcattgcttc catatctgtc ggtgttaagg actatgagta tgatttcgtt
     3661 agacagctca agactgatgc tcctgagtat atcactattg atattgctca tggtcatgcg
     3721 gatagcgtga tttctatgat tcaacacatc aagaaagaat tgccagatac ctttgtcatt
     3781 gctggaaatg tgggaacacc agaagctgtg cgtgaattgg aaaatgctgg tgcggatgct
     3841 actaaggtcg gaatcggtcc tggtaaggtt tgtatcacca aggttaagac tggttttggt
     3901 acaggtggtt ggcagttggc tgctctacgc tggtgtgcca aggctgcgcg taaaccgatt
     3961 atcgctgatg gaggaattcg tactcacggt gatattgcca agtctatccg cttcggtgct
     4021 agcatgatca tgattggttc cctctttgca ggacatattg aaagtccagg gaaaacgatt
     4081 gaagtcgatg gtgaacagtt caaagaatat tatggttcag cctcacaata tcaaaaaggt
     4141 gcttacaaaa atgtggaagg caaacgtatc ttgcttcctg ctaaaggtca tctgcaagac
     4201 actttaactg agatggaaca agaccttcaa agtgctattt cttatgcagg tggacgtcag
     4261 gttgctgacc ttaaacacgt tgattatgtg atcgtgaaaa actccatctg gaatggggat
     4321 gcttcccact aagacggggt ctttgactaa aaaacttgtt attagagcaa atttctgtta
     4381 taataaaaca agtttccacc cttagtgtaa tggatatcac gtaagattcc ggttcttgag
     4441 atgggggttc gattccctca gggtggatgt aaacattcta aaaagccttt aatagggctt
     4501 ttttctttac actactctaa atttgccact aaaactgaga atctctgtct aaagaattcc
     4561 caaatgcttt ccagtcccgt tttagagtga ctcagggggc tttttttgat ataataaaaa
     4621 ggactgttat cagttagaaa gaggttggta tgaaagaatt acaaactgta ctaaagaacc
     4681 attttgcaat cgaatttaca gacaaaaagt tactggaaac tgcctttact catacgagtt
     4741 atgccaatga gcaccgcctc ttaaaaattt cacataatga acgcttggaa tttttaggag
     4801 acgctgttct acagttattg atttcagaat atctgtataa aaaatatcct aaaaagcctg
     4861 aaggtgacct atcaaaactc cgtgctatga ttgtccgtga ggagagttta gctggttttg
     4921 cgcgtgattg ccagtttgac cagtttatca agttgggtaa aggggaagaa aaatctggtg
     4981 gtcgcaatcg tgacaccatt cttggtgatg cctttgaagc ctttcttggt gcccttcttt
     5041 tggataagga tgtggccaag gtcaaggaat ttatctatca agtcatgatt cctaaggttg
     5101 aagcaggcga atttgagatg attacagact ataaaaccca tctccaagag ttacttcagg
     5161 tcaatggtga tgtggctatt cgttatcagg tgatttctga aacagggcct gctcacgata
     5221 aggtctttga tgtagaagtt cttgttgaag gtaagagcat cggtcaaggc caaggtcgtt
     5281 ctaagaaatt agcagagcag gaagctgcca aaaatgccgt tgagaagggg ctggattcat
     5341 gtatttaaag gaaatcgaaa ttcaggggtt caagtctttt gctgataaga ccaaggtcgt
     5401 ttttgaccaa ggtgtgacgg cagttgttgg acccaatgga tctggaaagt ccaatattac
     5461 agaaagtctg cgttgggctt tgggggagtc tagtgtcaag agtctccgtg ggggcaagat
     5521 gccggatgtc atctttgctg gaacagaaag tcgcaaaccg ctcaattatg cttctgtagt
     5581 tgtgactctg gataatcatg acggatttat caaggatgca ggtcaagaaa tcagggtgga
     5641 acgccatatc tatcgtagtg gagatagcga atacaagatt gacggcaaga aagtccgtct
     5701 gcgtgatatt catgaccttt tcttggatac aggtttggga cgagattcct tctctattat
     5761 ttcccaaggg aaggttgagg agatttttaa ttccaagcct gaggagcgac gagctatttt
     5821 tgaagaagct gctggagttt taaaatacaa gactcgcaga aaagaaaccg agagtaaact
     5881 gcagcaaact caggataatc tggaccgctt agaggacatt atctacgagt tggataatca
     5941 aatcaagcct cttgagaagc aagctgaaaa tgcccgtaaa tttctagact tagaaggtca
     6001 acgcaaggct atttacttgg atgtattggt tgctcaaatc aaggaaaata aggcagaact
     6061 agagtcgaca gaagaagagt tggctcaggt tcaagaactc ttgatgagtt attaccaaaa
     6121 gcgtgaaaaa ttagaagaag aaaatcaaac tcttaaaaag caacgccaag atttacaggc
     6181 tgaaatggcc aaagaccaag gcagtttgat ggacttgact agtctgatta gtgatttaga
     6241 aagaaaatta gccctatcga aactggagtc cgagcaagtg gccttgaatc aacaggaggc
     6301 acaagctcgt ttggctgctt tggaggataa gagaaattca ctcagcaaag aaaagtctga
     6361 taaagaaagc tctttagctc tgttagaggg aaatctagtc caaaataatc aaaaactcaa
     6421 tcgtttagaa gctgaattgc tggctttctc agacgatcct gatcagatga ttgagctctt
     6481 acgtgaacgc tttgtagctc ttttacaaga agaagcggat gtctcaaacc agttgacccg
     6541 tattgagaat gagttggaaa atagtcgtca gctttctcaa aaacaagcag atcaactaga
     6601 aaagctgaaa gagcaattag ctatagctaa agagaaggct agtcagcaaa aggaagagct
     6661 tgaaactgcc aaggagcagg ttcagaaatt attggctgac taccaagcta ttgccaagga
     6721 gcaagaggag cagaaaactt cctatcaagc tcaacaaagc caactctttg accgtctgga
     6781 taatctcaaa aacaagcagg ccagagctca aagtttggaa aatatcctga gaaatcatag
     6841 taacttttat gcaggtgtta agagtgttct ccaagaaaaa gatcgcctag gtgggattat
     6901 tggtgcagtc agtgagcatc tgacctttga tatgcattat caaactgccc tagacattgc
     6961 gcttggagcg agtagtcagc acattattgt agaagatgag aatgcggcaa ccaaagccat
     7021 tgatttcctc aaacgaaaca gagccggtcg tgcaaccttt cttcctttga ccactattaa
     7081 ggcgcgtacg atttctagtc agaaccaaga tgctatcgct gtaagcccag gtttccttgg
     7141 gatggcagat gagttggtga cttttgatac tagactggaa gccattttca agaacttgct
     7201 agctacgacg gctatctttg ataccgtaga acatgcgcgt gcagcagctc gccaagttcg
     7261 ttatcaggtt cgtatggtga cattggatgg gacagaatta cgcacgggtg gttcctatgc
     7321 gggtggtgcc aatcgccaaa ataacagtat tttcatcaag ccagaactgg agcaattaca
     7381 aaaagaaatt gctgcagatg aagcaagctt gcgttcagaa gaagtggctt tgaagacctt
     7441 gcaagatgag atggccagat tgaccgagtc attagaagcc atcaaatctc aaggagagca
     7501 ggcacgtatt caggagcaag gcttgttcct cgcttatcag caaactagtc agcaagttga
     7561 agaactggaa actctttgga aactccaaga agaggaaaaa gatcgtcttt ccgagggaga
     7621 ttggcaagca gacaaggaaa actgccaaga gcgtcttgct gcaatcgcca gtgacaagca
     7681 aaatctggaa gctgagattg aagagattaa gtctaacaaa aatgccatcc aagaacgcta
     7741 tcaaaacttg caggaagagc tagcgcaagc tcgtttgctt aagacagaac tgcaagggca
     7801 aaaacgttat gaagtgactg atattgaacg cttaggtaag gaactagata atcttgatat
     7861 tgagcaagaa gaaattcagc gccttcttca agaaaaggtt gacaatcttg agaaagttga
     7921 tacggatttg ctcagtcaac aggctgatga agccaaaact caaaaaacaa atctccaaca
     7981 aggtttgatt cgcaagcagt ttgagttgga tgatattgag ggtcaactgg atgatattgc
     8041 tagtcatttg gatcaggctc gccagcagaa tgaagagtgg attcgcaagc aaactcgtgc
     8101 tgaagcaaag aaagaaaagg ttagcgagcg cttgcgccat ctacaaagtc aattaacaga
     8161 ccagtaccag atcagctaca cagaagcttt agaaaaggcg catgagttgg aaaatctcaa
     8221 tctggcagag caagaagtta aggatttaga gaaggctata cgctcactgg gtcctgtcaa
     8281 tatagaagct attgaccagt acgaagaagt tcacaaccgt ctggatttcc taaatagcca
     8341 acgtgatgat attttgtctg cgaaaaacct gcttcttgaa accatcacag agatgaatga
     8401 tgaggtcaag gaacgcttta aatcaacctt tgaggccatt cgtgagtcct ttaaagtgac
     8461 cttcaagcag atgtttggtg gaggtcaggc tgacctcatt ttgactgaag gagacttgct
     8521 gacagctggg gttgaaattt ctgtccaacc accaggcaag aaaatccagt ctcttaatct
     8581 catgagtggt ggtgaaaaag ccctatcggc tcttgccttg ctcttctcta tcattcgagt
     8641 taagactatc ccgtttgtta tcttggatga ggtagaggct gcgctagacg aagccaatgt
     8701 taaacgtttc ggggattatc tcaaccgctt tgataaggac agtcaattta ttgtcgtaac
     8761 ccaccgtaag gggaccatgg cagcggccga ttctatctat ggagtgacca tgcaagaatc
     8821 gggtgtttca aagattgttt cagttaagtt aaaagattta gaaagtattg aaggatgaca
     8881 attaaactag tagcaacgga tatggacgga accttcctag atgggaatgg acgctttgat
     8941 atggatcgtc tcaagtctct cttggtttcc tacaaggaaa aagggattta ctttgcggtg
     9001 gcttcgggtc ggggatttct gtctctagga aaattatttg ctggtgttcg tgatgacatt
     9061 attttcatcg cggaaaatgg cagtttggta gagtatcaag gtcaggactt gtatgaagcg
     9121 actatgtctc gtgactttta tctggcaact tttgaaaagc tgaaaacgtc accttatgta
     9181 gatatcaata aactgctctt gacgggtaag aaggggtcat atgttctaga tacggttgat
     9241 gagacctatt tgaaagtgag tcagcattat aatgaaaata tccaaaaagt agcgagtttg
     9301 gaagatatca cagatgacat tttcaaattt acaaccaact tcacagaaga aacgttagaa
     9361 gctggtgaag cttgggtcaa tgataatgtc cctggtgtca aggctatgac aactggcttt
     9421 gaatctattg atattgttct ggactatgtc gataagggtg tagctattgt tgaattagct
     9481 aaaaaacttg gtatcacaat ggatcaggtc atggcttttg gagacaatct taatgactta
     9541 catatgatgc aggttgtggg acatcctgta gctcctgaaa atgcacgacc tgaaatttta
     9601 gaattagcaa agactgtgat tggtcaccat aaggaacggt cggttatagc ttatatggag
     9661 ggcttataat ggcagatata aaattgattg cattggactt ggacgggacc ttgctgacta
     9721 ctgataaaag gctgacggat cgtaccaagg aaaccttgaa agctgcgcgt gatcgtggta
     9781 tcaaggtcgt attgacaact ggtcgtccct taaaagccat ggatttcttt ctccatgagt
     9841 tagggactga cggtcaggaa gatgagtata ccattacttt taatggtgga ttagttcaga
     9901 aaaatacagg agaaatcctt gataaaacag tcttttcata tgatgatgtg gcacgtttgt
     9961 atgaagaaac agagaaatta tcactgcctc ttgatgccat ctcagaagga acagtttatc
    10021 aaatccaatc ggaccaagaa agtctttatg ccaaattcaa tccagccttg acctttgttc
    10081 cagtggactt tgaagactta tctagtcaaa tgacctacaa caaatgcgtg actgcctttg
    10141 ctcaagaacc cttggatgca gccattcaga agatttctcc agaattgttt gaccaatatg
    10201 aaatctttaa atcacgtgaa atgttgctag agtggtcacc aaagaatgtt cataaagcaa
    10261 caggtttggc aaaactaatt agccatcttg gaatcaacca aagccaagtt atggcctgtg
    10321 gtgacgaggc caatgacctc tctatgattg aatgggcagg gctcggtgtt gccatgcaaa
    10381 acgctgttcc tgaagtcaag gcagtcgcaa atgtggtaac tcctatgacc aacgatgagg
    10441 aagctgtcgc ctgggctatc gaagaatatg tgctaaagga gaactaagat atgggattgt
    10501 ttgaccgtct attcggaaaa aaagaagaac ctaaaatcga agaagttgta aaagaagctc
    10561 tggaaaatct tgatttgtct gaagatgttg atcctacctt cacagaagtt gatgaagttc
    10621 ctcaggaaga agcagaggtt gaaattgttg aacaagctgt gttccaagaa gaggaaatcc
    10681 aagacacagt tgaagaaagt ctggattcag agccagttgt aggggtttct caagaagaag
    10741 tcgaagaatt tccacactca gaagaattac cagaggaaga gaagcttgat cacgaaggaa
    10801 ctatagaaga aaataattct gaagttcttg aagcagaaag gcctcaagca gaagaaaccg
    10861 ttcaggaaaa atatgaccgc agtcttaaga aaactcgcac aggtttcggt gcccgcttga
    10921 atgccttctt tgctaacttc cgctctgttg acgaagaatt tttcgaggaa ctggaagaac
    10981 tgctgattat gagtgatgtt ggtgtccaag tcgcttctaa cttaacggag gaactacgtt
    11041 acgaagccaa gcttgaaaat gccaagaaac ctgatgcact tcgtcgtgtc atcattgaga
    11101 aattggttga gctttatgaa aaggatggta gctacgatga aagcatccac ttccaagata
    11161 acttgacagt tatgctcttt gttggtgtga atggtgttgg gaaaacaact tctatcggaa
    11221 aactagccca ccgctacaaa cgagctggta agaaggtcat gctggttgca gcagatacct
    11281 tccgtgcggg tgcagtagct cagctagctg aatggggccg acgagtagat gttccagtag
    11341 taactggacc tgaaaaagct gatccagcca gcgtggtctt tgatggtatg gaacgtgccg
    11401 tggctgaagg tatcgatatt ctcatgattg atactgctgg tcgtctgcaa aataaggata
    11461 accttatggc tgagttggaa aagattggtc gtattatcaa acgtgttgtg ctagaagcac
    11521 cacatgaaac cttcttggca cttgatgcat caacaggtca aaatgcccta gtacaggcca
    11581 aagaattttc gaaaatcaca cctttaaccg gaattgtttt gactaagatt gatggaactg
    11641 ctcgaggagg tgtggttcta gccattcgtg aagaactcaa tattcctgta aaattgattg
    11701 gttttggtga aaaaatcgat gatattggag agtttaactc agaaaacttt atgaaaggtc
    11761 tcttggaagg tttaatctaa tcagaagcaa aaatcctgca aggcataaac ttgcaggaaa
    11821 tttttttatt ctaagcgacc atcttgacga taggcgatat ctggttgcca agtccatttg
    11881 gcaccgaatt tttcaagtag gtcaaagctg gcttgaggtc ccatgcttcc agctttatag
    11941 tcatgaagtg gggcaccatt ttcagcccag agcttttcaa tacggtcaat taacttccat
    12001 gacgcaccaa cttcatccca gtggctaaag ttagttgagt tgttatttag gacatcataa
    12061 atcaattttt cgtatggttc tggagaagca ccagttgcag tcgcagctgt acggtaatca
    12121 agtgagttag gagccaagtt aaattcttct cctacttgct tcccatttag gctaagagag
    12181 aagccttctg ttggttgaat atagatggtc aaaatatttg gagcaagtgg ttctccaaat
    12241 atagaatcca tttgtttaaa gacgatgttg acatgagttc ctttttcagt cagtcgttta
    12301 cctgtacgga aaaagaaagg aacactacgg aatcgatcgc tgtctacaaa gaaggcacca
    12361 gatgtaaagg tttcagttgt tgattctgga ttcacatttg gctcgctacg ataagagatg
    12421 tatttcatgc catcaatctt accagagcgg tattgcccac ggataaagtg ttctttgagt
    12481 tcttcatcag ttggatgata gaggttttta aagaccttaa tcttttcagc acgaatctcg
    12541 tcttttgtga agcttgctgg tttgtccatg gcgaggagcg aaagaagttg tagagtgtgg
    12601 ttttggacca tgtcacggag ggcaccggat tggtcatagt agccaccacg ttcttctaca
    12661 cccaagcgct ccgcaaaggt aatttgaaca ttgtcgataa aatccttgtt ccaaacgttt
    12721 tcaaaaatca agtttgcaaa gcgaactgca aagatgcttt ggatcatttc cttaccaaga
    12781 taatggtcga tacggaaaat ttgttcttcg tcaaatgttg ctaggagttc gtcattcaac
    12841 ttgcttgcag ttgcgtaatc tgtaccaaat ggtttttcaa cgatcaagcg ctcaaaacct
    12901 ttgccatcga caatgttttc agatttgagg tgtttggcaa tggttccaaa gaactgaggt
    12961 gccatagaca agaagaagag cttattgtgt tcagcttggt atttttcatt aagctcagct
    13021 tgtaattgac gcaaagcaat ataatgttcc gaatcattga catcatggct ttgatagtag
    13081 aagtggctag caaattcttg ggcttgctcg gtactatctg ccaaatcaag gatggactcg
    13141 acaactacag attcaaaata ttccttactc caaggtctac gggcagttcc aataacggca
    13201 aagtgcttgg aaagattgcc ggatttatat agtctaaaaa gggaagggta gagcttgcgt
    13261 ttagccaggt ctccactcgc accgaaaatt gtaacaataa ccttagatga catctagcta
    13321 cctaatttct atttttattc ctagtcttgc taggtatgag gaaacctcat ttcataaact
    13381 taattctaac ataattttcc gaaaaatcaa aaattccaat acgaggtaga aaaactgcaa
    13441 ggaaatcctt acagtttgag tttagacgtt taagacctta tctaagaact ctttcagacg
    13501 agggtgttgt gggttatcaa agatttggtc aggtgttccg tcttcaagga actcgccatc
    13561 tgcagtaaag ataacgcggt tggcaacctg acgagcaaat cccatctcat gggttacgat
    13621 aatcatggtc atgccttgct cagccaattc cttcataacg ttaagtacgt ctccaaccat
    13681 ctcagggtca agggcagaag ttggttcatc gaagagcatg atgtctggat tcattgctag
    13741 gccacgagcg atggccacac gttgtttttg accacctgat aggctatctg gattggcatt
    13801 agctttatct gctagtccaa ccttttcaag caactccatt cccaattcct cagcttcttc
    13861 cttagtcatc aacttgtgct caataggagc aaaggtgatg ttgtccaata cagacatatg
    13921 agggaagagg ttgaagtgtt ggaataccat gccgatattt tcacggacgt ggtcaacatt
    13981 ggttgttttt tcagttaaat catagccgtt cacagtgatg tgaccgctag tgacttcttc
    14041 taaaagattg aggctacgga ggaaagttga cttaccagaa cctgaaggac cgatgataca
    14101 aacaacatct ccttcataga acttagtcgt aattcctttt aggacttcat tttttccata
    14161 gtgcttgtgt aaatcattta catcaatttt taattttgcc attaacgaat cctcttttct
    14221 aagcgtttcg ctagtctagt caaaagtgtg ataattacaa gatagaagat agcaaggatt
    14281 gcatacatct tgaaactttg gtagttacga gcaatgataa tcttaccagt ttggaagagt
    14341 tcaaccaaac cgatagcaga tacgatagtt gtatctttaa gagcgataac gaattggttg
    14401 acaaagtttg gcaacatcaa tttagttgct tgtggcaaga taatcttacg catggttttt
    14461 ccataagaga tacccaagct tcggctggct tccatttggc caactggaac ggcctgaata
    14521 ccaccacgaa cgatttcagc gatataagca gccgcattga gtgagagggc aattgttcca
    14581 gctacaaagt cgttaattgg gctttgttgg cctgtgatag actcgatgaa gtttggaatt
    14641 ccccagaaga tgaaggctgc aagaatcatc aatggaatac cacgaataac gtcaacgaaa
    14701 atctcagaga tgacgcgaag agatttgtat gggctaacgc taaacatacc gaagataatt
    14761 ccgatgacaa tggcaatagc aaatgagata agagctagag caagagtgat accaagaccg
    14821 ctaaggagtt gtttgtagtt gttttgaagc aagccccaga gcgttgtttc gtcaacagta
    14881 cttgttgaag cagttgaaga ttcgctagct aggtatttgt caagaatctt ttggaattca
    14941 ccgtttgctt taaggtttgc aagtccgttg ttgaacattt caatcagttc tggatttgct
    15001 ccttttttaa cggcaaaggc tgtttcaccg attggagttc cagagattgg agttttcaat
    15061 ttttgacctt ggctgataga atatttgaga acaggttcat catccataac ggcatcaatg
    15121 gcaccagtat ttaaactgtc atacattgaa gaaccatcag caaaggtttt gattttgtag
    15181 ccgtatttgc tttgattttc tgttaggaag gtttgagaag cagttccgtt tttaacaccg
    15241 actgtctttc cttttagatc ttcataagaa gcaatattgc ttgattcttt gacaccaaga
    15301 atggtattag cagtgtagta tgattctgag aagtcaaaag ttgccttacg agcatctgtg
    15361 acagacatac cagcgatgat accatcggct tgaccagctt ggacagcact gatagcagca
    15421 tcaaaaccag ggttggtgat ttcaatttca aaaccttggt ctttagcgat tgccttaatc
    15481 aattccatat caataccagt gtattggttg cttgaatttt ggaaaacaaa aggtgcaaaa
    15541 gaagaatcgc tggcaatgat atatttagcc ttaacaggaa tggcttttaa tcctgcgaga
    15601 gtagttgtag ttggcactgc tgaagatgat gaagcagtcc atttcttgat aattttatca
    15661 agactaccat cttttttcat ttcagacaag gcttggttaa attcagtaac caggtgctcg
    15721 tatttacttc cttttttcac accgaaagca aaacttccta cagcttcacc atccatttca
    15781 atatggaggt cttgaccttg gttaatggca tattcgataa caggtttgtc atccatcatg
    15841 gcatcgatgg caccagcact caagctgttg ttcattaaat caccagtgtc aaatgtttta
    15901 atagtaaagc cgtatttatc tttgattgtt tcaaggaaac gttgagcggc agttccgttt
    15961 ttaacaccaa cggttttgcc agttaattgg tcgtacttgc taattttgtg tgactttgta
    16021 gtagcaatga caacttttgt atcatagtaa gtatcagaca tggtgaagac tttttcacgt
    16081 tctttagtct ttgtcatccc tgccatgata gcgtcggctt gcccagcttg aaccgcattg
    16141 actgctgcgt caaatccagg ataggacatc tgaatgttcc agcctttaat ctcagcgact
    16201 ttgttaataa tgtcaacatc aattccttta taagtttgat ctgaatcttt aaactcaaaa
    16261 ggtgcatagg cggtatcaga aacaatctta atcgtttctg ctttggcaat acctaatgag
    16321 aaaattggga ataaaattag caaaaatgct agaaattttt tcttcatttt tagtctcctt
    16381 ttc
//
LOCUS       DAVMGZ010000032        15694 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000032 DAVMGZ010000000
VERSION     DAVMGZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15694
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.033"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..999
                     /locus_tag="VYQ93_000864"
     CDS             <1..999
                     /locus_tag="VYQ93_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV3335580.1"
                     /translation="RRFKCYHCSKMMVAETSIVKKNHQIPRIINQKIAQKLIEKISMT
                     DIAHQLSISTSTVIRKLNDFHFKHDFSCLPEIMSWDEYAFTKGKMSFIAQDFNNLNII
                     TVLEGRTQAVIRDHFLKYDRAVRCRVKIITMDMFSPYYGLAKQLRFHIVQHLSRAMSR
                     VRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSDKQFYRPTFRMHLTNKEILNKLLS
                     YSEDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVN
                     ALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(1099..2721)
                     /gene="groL"
                     /locus_tag="VYQ93_000865"
     CDS             complement(1099..2721)
                     /gene="groL"
                     /locus_tag="VYQ93_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV3335581.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISNRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2737..3021)
                     /gene="groES"
                     /locus_tag="VYQ93_000866"
     CDS             complement(2737..3021)
                     /gene="groES"
                     /locus_tag="VYQ93_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV3335582.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3291..3686)
                     /locus_tag="VYQ93_000867"
     CDS             complement(3291..3686)
                     /locus_tag="VYQ93_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV3335583.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3763..4524)
                     /locus_tag="VYQ93_000868"
     CDS             complement(3763..4524)
                     /locus_tag="VYQ93_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV3335584.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTHCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(4557..5183)
                     /locus_tag="VYQ93_000869"
     CDS             complement(4557..5183)
                     /locus_tag="VYQ93_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV3335585.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVACLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPVKHWTA"
     gene            complement(5199..5516)
                     /locus_tag="VYQ93_000870"
     CDS             complement(5199..5516)
                     /locus_tag="VYQ93_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV3335586.1"
                     /translation="MIQPASLEELASLVEKDGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5513..5812)
                     /locus_tag="VYQ93_000871"
     CDS             complement(5513..5812)
                     /locus_tag="VYQ93_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV3335587.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            5900..5980
                     /locus_tag="VYQ93_000872"
                     /pseudo
     CDS             5900..5980
                     /locus_tag="VYQ93_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(6109..6300)
                     /locus_tag="VYQ93_000873"
     CDS             complement(6109..6300)
                     /locus_tag="VYQ93_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV3335588.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6461..7135)
                     /locus_tag="VYQ93_000874"
     CDS             complement(6461..7135)
                     /locus_tag="VYQ93_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335589.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKIVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(7141..7587)
                     /locus_tag="VYQ93_000875"
     CDS             complement(7141..7587)
                     /locus_tag="VYQ93_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3335590.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7701..8204)
                     /locus_tag="VYQ93_000876"
     CDS             complement(7701..8204)
                     /locus_tag="VYQ93_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV3335591.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVTLSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(8201..8563)
                     /locus_tag="VYQ93_000877"
     CDS             complement(8201..8563)
                     /locus_tag="VYQ93_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV3335592.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8629..10488)
                     /locus_tag="VYQ93_000878"
                     /pseudo
     CDS             complement(8629..10488)
                     /locus_tag="VYQ93_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10527..10976)
                     /locus_tag="VYQ93_000879"
                     /pseudo
     CDS             complement(10527..10976)
                     /locus_tag="VYQ93_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(11100..11816)
                     /locus_tag="VYQ93_000880"
     CDS             complement(11100..11816)
                     /locus_tag="VYQ93_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3335593.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12697..14112)
                     /gene="ply"
                     /locus_tag="VYQ93_000881"
     CDS             complement(12697..14112)
                     /gene="ply"
                     /locus_tag="VYQ93_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV3335594.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(14123..14455)
                     /locus_tag="VYQ93_000882"
     CDS             complement(14123..14455)
                     /locus_tag="VYQ93_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335595.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTGIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14526..15134)
                     /locus_tag="VYQ93_000883"
     CDS             complement(14526..15134)
                     /locus_tag="VYQ93_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335596.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(15147..15605)
                     /locus_tag="VYQ93_000884"
     CDS             complement(15147..15605)
                     /locus_tag="VYQ93_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV3335597.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
BASE COUNT         4578 a         3615 c         2837 g         4664 t
ORIGIN      
        1 cgtcgattca agtgttatca ctgttcaaaa atgatggtcg ctgaaacttc tatcgtcaag
       61 aagaatcacc aaatccctcg tattatcaac caaaaaattg cgcaaaagtt aattgaaaag
      121 atttctatga ccgatattgc ccatcagctt tccatctcaa cttcaactgt tattcgtaag
      181 ctcaatgatt ttcactttaa acatgatttt tcttgtcttc ctgagattat gtcttgggat
      241 gagtatgctt ttacaaaggg aaagatgagc ttcattgcgc aagattttaa caatctcaat
      301 atcatcactg ttcttgaggg aagaacacaa gctgtcatcc gagatcactt tcttaaatat
      361 gatagagccg tccgatgtcg cgtcaaaatt attactatgg atatgtttag cccttactat
      421 ggcttggcta aacagcttcg ctttcacatt gtacaacatc ttagccgtgc tatgagtcgt
      481 gtgcgtgtcc aaatcatgaa tcagtttcat cgaaaatccc atgaatacaa ggctatcaag
      541 cgctactgga aactcattca acaggatagc cgtaaactga gtgataagca attttatcgt
      601 cctacttttc gtatgcattt aaccaataaa gagattttaa acaagctttt gagctattcg
      661 gaagacttga aacatcacta tcagctctat cagctcttgc tttttcactt ccagaataag
      721 gaaccagaga aatttttcgg acttattgag gacaatctta agcaggttca tcctattttt
      781 cagactgtct ttaaaacctt cctcaaagat aaagaaaaga ttgtcaacgc ccttcaacta
      841 cactattcta atgccaaact ggaagcgacc aataatctca tcaaacttat caagcgcaat
      901 gcctttggtt ttcgaaactt tgaaaacttc aaaaaacgga ttttcatcgc tctgaatatc
      961 aaaaaagaaa ggacaaaatt tgtcctttct cgagcttagc ttttcttcaa cccactacag
     1021 ttgacaaaga gcctataaaa agaagggtta gtcattccct ccttttgtgt tttttataag
     1081 ttgttttcta tagaaagctt acatcatccc gcccatcata cttggatcca ttgctggagc
     1141 tggggctact ggttctggtt tattggctac gactgcttct gttgtcaaaa tcaagctggc
     1201 tacagatgct gcattttgta gggctgaacg actcacttta actggatcaa tgataccttg
     1261 atcaatcatg ttaacccact cgccagttgc tgcgttgaat cctataccaa gctcagcatt
     1321 tttcaaacga tcgataacga tagatccttc aaatcctgca ttgtgagcaa tttgacgaac
     1381 gggttcttcc aaagcacgga gaacaatatt acgtcctgtt gcttcatctc ctgtcaattc
     1441 caaggtagca acagctggaa tcacattggc aagagctgtt ccaccacctg caacaatacc
     1501 ttcttcaaca gctgcacgag tagcgttgag ggcatcttca atgcggagtt tcatttcttt
     1561 caactcagtt tcagttgcgg ctccgacctt aataaccgct acaccacctg acaatttggc
     1621 caagcgttct tgcaattttt cacggtcaaa ttcagaagtt gtagtttcga tttgagactt
     1681 gataaccgca acacggttag aaatcgcttc aggatttcct gcaccttcta caataaccgt
     1741 gctatctttg tccacggtca ctctcgctgc ttgaccaaga gcttcaattg tcgcatcttt
     1801 caactcaaga ccaaggtctt ctgtgataac tgttccgcct gttaagatgg cgatatcttc
     1861 aagcatggct ttgcgacggt caccaaaacc aggtgccttg actgctacta cgttgaaggt
     1921 tccacgaatc ttgttcaaaa caagagttgg aagagcctcg ccatccacat catccgcaat
     1981 aatcaagagt ggacgattgc tttggagaat gctttccaaa agtggcaaga tttcttggat
     2041 attggaaatt ttcttgtctg taatcaaaat gtacggattt tcaaggtcag ccaccatttt
     2101 ttcgctatca gtcaccatgt actgtgaaag gtaaccacgg tcaaactgca ttccttctac
     2161 gacttcaagc tctgtttcca taccacgtga ctcttcgatg gtgatgacac cgtctttgcc
     2221 aactttttcc attgcttcag agatgtactc accaactttt tcagaacgag aagatacggc
     2281 tgcaacttga gcgatagctt ctttattggc aacagggatg gcgttgtttt tcaaagcttc
     2341 aactgctgcg gcaactgctg tttcaatccc acgacgaata ccgattggat ttgcacctgc
     2401 tgtgacgttt ttgattcctt cacggacgat agcttgggtc aagactgttg cagtcgtagt
     2461 cccgtcacct gcgatatcat tggttttaga agctacttct gatactaact tagcacccat
     2521 attttcaaaa tggtcttcca attcgatttc tttggcaatg gtcacaccgt cattggtaat
     2581 caagggtgaa ccgaatgact tttcaagaac gacattgcga ccttttggtc ccaaggttac
     2641 tttaacagtg tctgcaagaa tatcgacacc acgaaccatg gctgaacggg catctgatga
     2701 aaatttaatt tcttttgaca tacttacttt ctccttctat tcctcaatga ttgccaaaat
     2761 gttagcttcg cctacgataa tgtacttttc atcgccatct ttgacatcaa gacctgcgtg
     2821 ggcttcaact aagacacgat ctccagtttt aacacttgga gcaaccaagt caccgttcaa
     2881 ggtacgaaca ccttgtccag tagccacaac ttgagctgtt ttggtttttt cttgggctga
     2941 gcctgcaagg acaaagcccc caacggtttg ttctttttct tctattttta agaccacacg
     3001 gtcccctaat ggtttcaaca tctgatttcc tccataatga gatagatatt attagcactc
     3061 tttgaactgg agtgctaatt cataattcta ttgtatcact tggtcagaaa tagtcaagaa
     3121 aaaagtctga ctttctcaag ataaaaagcc tgagaccaac tcagactttt taatgtttat
     3181 actcaatgaa aatcaaagag caaactagga agctagccgc aggctgctca aagcattgct
     3241 ttgaggttgt ggataaagct aacgtggttt gaagagattt tcgaagagta ttaaaatggc
     3301 aattcttcct cttccaagac caaatctgcc aaatcttggc ctgcattatt ttcacgcatg
     3361 gcacgttgag cacgactttc taagagttgg aatcctgtca caaggacttc ggttacatag
     3421 ttcatctgac catttttctc aaagcgacgg gtacgcaatt ctccatcaac ggaaatgaga
     3481 ctacctttgg ttgcgtagct tgccaaagtt tctgctagtc tgccccatag gaccatattg
     3541 acaaaatcag cttcacgttc accgttttgg tctttgtaac gacggttgac agcgatagtt
     3601 gctcgcgcta ccgacttgtc attgttggtt ttgtgcaatt ctggtgtaga cgttaaacgc
     3661 ccaatcatga taactttatt atacatattt tcttcctcct acttatctat tcgtaggaaa
     3721 tcaaaaaagt tacagaaatt tgtaactttt cgaggaaatt tcttattttt tatgaatcat
     3781 gaaacctgtc gcctgttgat tggccataat ggtcatatct gtaatctgaa cacggcgagg
     3841 ctgactggtc acatagacta ctgtgtctgc aatatcctga gcttgcaagg cttctattcc
     3901 ttggtaaacg gacgcagccc gctctttatc accatgaaaa cgaacagttg agaaatctgt
     3961 ttcgacaatc ccaggctgaa tggttgtcac cttgatatcc gttgcgatgg tatcaattcg
     4021 cagtccatcc gaaaaggtct taaccgctgc cttggtagct gagtaaacag cggcaccagc
     4081 ataggcgtag attcctgcgg ttgaccccat attgataata tgaccttgat tgacttttat
     4141 cattgctggc aagaaacagt gagtaaccgc catcagacct ttaacattgg tatccaacat
     4201 ggtcaacata tccaactcct catagtcttg atagggagcc aagtcaagag ccagtccggc
     4261 gttgttgacc aagatatcaa tcctccctat cgtttctaga atatcagagc aaacagtctt
     4321 caccatgacc atatccgtca catctagtgg aaaagtccag acggtttgat ttggaaagct
     4381 tactgcaaac tccgacttga ggatttctaa tctgtctatc cgtcgtcctg ttagaacgac
     4441 atcctcaccc tgctccagat aagcacgcgc aatcgcttca ccgattcctg aggttgctcc
     4501 cgtaatcact acattttttg ccatcttatt tccttctatc agatgctgac aagttcctag
     4561 gcagtccagt gtttcactgg gtcgaacggt gttccgacaa cttggtcttc tgataattca
     4621 agcaccccac gtttttgcgg agcatttggc aaatgcaatt cacgaggact acacatcatc
     4681 ccaaaactct tttcaccacg aagttcgcct gggaaaatga aattcccttt tggcatcatg
     4741 gcaccaggaa gagccacaat ggttttcaac ccaacacgcg cattaggtgc tcctgcaacg
     4801 atttgcacta tcttgtcact tgcgactgca acttggcaga tgttgaggtg gtcactatct
     4861 ggatgggcta ccatctcaac aatttcacca acaacaaact taggttcctt gtcattgaca
     4921 atttcttctg taaaaccttc cgcctgtaat tcttggttca aacaagcgac ttgctcatct
     4981 gataaaaaga cttgacctcg ctctgcaatt tcaaacaagc tcgaaacctc aaaaatgttc
     5041 caagctactg tttccccatt ttctttgagg aaaacacggg ctaccttgcc tttgcgttcc
     5101 acattcagtt tggcatctcc gctatttttc acgatgacca taaggacatc accgacatgt
     5161 tctttgttat atgtaaaaat cattgtttac tttttctcct atttcaatcc tgctaaaaaa
     5221 tcgttaattt gctccttact tttacggtcg cgattgacaa aacggccgat ttccttgtcc
     5281 ttttctagaa caacaaggct aggaattccg taaacatccc agagtttggc caaatccata
     5341 tactgatctc ggtccattcg aataaaggtg aactctggat tggtctcctc aatctctggt
     5401 aaggcaggat agatataacg gcaatcgcca caccagtccg ccacaaaaag gaagaccttc
     5461 ttaccgtctt tttctactaa agatgccaat tcttctaaac ttgctggttg tatcataaga
     5521 cttcctcctc atagactagg tcttcatttt catagacaaa agtataatga cggccatcct
     5581 caaaaatgac gccaccaagc aagctctcta gactgctttc atagacttga acatagaggg
     5641 tcgcaatttc ccccatgtct gaaaaatggt ctcgtacaat cgctgtcaac tcttcctgag
     5701 tcttcatgag cttatggtca tctgcaactt ttttcgtagc aagggcaagg ctcccaatac
     5761 caagcagagc cagacctgcc atccacattt ttttagcttt cataccattc attttaacac
     5821 aaaaaaggct ttaggacaaa tgaggaaaca gcaaaaaagc aagtaaaaag cctcttcctt
     5881 taaggaaaag gacttcttat actcaatgaa aatcaaagac caaactagaa agctagccgc
     5941 aggctgctca aaacacggtt ttgaggttgt agataaggcg acgctgacgt ggtttgaaga
     6001 gattttcaaa gagtattatt cttattgcca ggcacctaag ttgccaacgt agtaaccatc
     6061 aggtgtgtag gtattgcgaa gcatcttacc tgatgaagcc agataatact acttgccatt
     6121 gtctttgacc caatcattcg caatcatgga accagaagaa cttacataat accattctcc
     6181 cttgtcataa acccaagtac tgactttcat ggttcctgag caattaaagg caaaaaaact
     6241 gtccaatatc attcgttttt taaaagcatt tgacactaca tcatctacgc tgtagtacat
     6301 agatacatca aacgtaaaag ccaaaatatc gaaaaatata caacttttaa aggcagcatt
     6361 tcatttttta aagtctaaca accatagaac tgaaatcaga gcaaggcatc aatttcctac
     6421 tcttagtacg tctattttga tggttgaagt gaagattttt ttatttaacg tagacaattt
     6481 ccaacttccc aaaagccaca tctccacgga tagtcaaggt tttgctggtt ggggctacag
     6541 gagcgtcagc cttagctgca ccaaaggagg tttctacttt caaatctaca cgccagtgtt
     6601 gtggaacata gacggttgca tttccgaagg ccacttcaat atttaaagtt gcaaaatcac
     6661 ctagcatctc tgcattatca tagtagattt tagcatcccc aaaagcgact tccacttggt
     6721 catctacgag atcttgatct tgcttataga aggtcccgct accaaaagcg acttccttat
     6781 ccgtgacgac tatttttttc ccattgtacc accatttttt ttcattccag aacttacttg
     6841 aatgcgtcag ataaccaaca ccaagtacca ccaagatgct agcccaaata agagaatgat
     6901 tggtaactgg taacaagtca taagcgtaat ttgcaatgat gagcgccagt aaacctgtaa
     6961 aaactgccga agtgagatga cgtctaagga tggactcaat ggacttataa gcaaaaaaaa
     7021 caatacctag taaaggccat attttaccat ccaaagaagg aattccaaaa ttcccttgca
     7081 gcaagatcca agctgctaaa accaataaaa caataccaaa tgctttcttt ttcatgtttt
     7141 ttacctcatg tttcttagat tttcttttag gagggattgg taatgccgtg agacatgaac
     7201 ctccttgtgc gtctgataaa aggaaatggt gcccgttcct gaaaaggact tgtccactga
     7261 gtaaatctga cggatgtttg cgatcgttga cttggaaact cgattaaaat agcgaggcaa
     7321 gatagactcc aactcataga gcttgaggcg aacctcataa gcttccttct ggttatgagc
     7381 gtagatcttg ctcccttctg tttcaaagaa gagaatttct gacaggtcta gataatattc
     7441 acctgtcccc ttgtaaaaag tcaacctcgg agcctttgac tcttgcaaga gccgttgcaa
     7501 gtctgcaatt tcatctgtca aagtgggggt cttgatgaca atttcagtct cctctaaatt
     7561 gccgtcaatc tcaattcgta acttcatcac atctcctttc tgactctact atatcaaagc
     7621 taagttagga atacaagggc aaaaaagcaa gtggtcaatt tgaagcagta agtggttgta
     7681 ggactagctt aacttacatg tcagaatagg aagaaaagga atccccacac caaaccacaa
     7741 gcatagccaa ccaagacatc cttgggataa tgaacacccc ctaaaaccct caccaacccc
     7801 agcaaagctg aaaagatcaa gcatactaag ccaataggta gactagcatg cagacaggcc
     7861 atggagatga tagttgccga aaagacatgg cgactgggca tcgaccgtcc cgaactatcc
     7921 ttatcaagaa ggggacagat atcccaagtt tcataaggtc tcgggtgatt gattttctta
     7981 cgaaacaggg acaaggtcac aaaacctgat gcaggaataa gcacataaac cccaacttgc
     8041 tgaccaagtc ctagttgcag gtaagtggtg actagcaaga tcagatagac caggggcatg
     8101 gcgactgtca tcaaacgatt gaaactgcgt aataggaata aaagaattgg acgactagtt
     8161 agtctggagc tgatatttcg ataccattct tgataatttt tcatttatgc ttctcatact
     8221 tttctagaac ttgttgagat gttttcccct tggcaagcca atccactagc ttgtccaagc
     8281 gacgtatttc ctgcatcaga ggatcatcaa tttcctcaat acggacaccg caaatcttcc
     8341 ctgtaattgc aattcgttca gggacataat atggtgcttg tctaaaaaaa tctccgtaag
     8401 taacatccct atccagacaa gcaagaacat cactcacttc ataccctgtc aaccaactgg
     8461 tcacttggtg aactgactct gcctttccac cctttcgctc aacttttgca atcaaggcta
     8521 agtagacagc agcaaatttc atattgtata atttctgact catacttctc tctttctatg
     8581 tgtactagta taaataagaa aaagaaggcc gtcaagcctt cttttgattt attcttctgc
     8641 ttcatcttct gtaaattgac tattgtacaa gtcagcgtag aagccacctt gcgccatcag
     8701 ttcctcatag ttgccttgct cgatgatatt tccatctttc atgaccaaga tcaagtctgc
     8761 atttcggatg gttgacaagc ggtgggcaat gacaaaggat gtgcgtcatt ccatcaaacg
     8821 gtccatggct ttttggatca attcctctgt ccgtgtgtca acagaagaag tcgcctcatc
     8881 caaaatcaaa agcggtgcat ccttaagaag ggcacgagca atagtcaata gttgtttttg
     8941 tcctacagac aaggtcacgg tgtcatccaa gatggtatca tagccatctg gcaaggtcat
     9001 aataaagtgg tgaattccca cagccttact agcttccatc attcgttcat cactaatccc
     9061 tatttgatta tagatgagat tgtctcgaat agttccttca aagagccagg tatcctgcaa
     9121 gaccattgaa aaggcatcat gcacttctga acgcgtcata gccttggtat ccacaccatc
     9181 aatgtgaata cttcccttat caatctcata gaatttcatc aaaagattga caatggttgt
     9241 cttaccagtc ccagtcggcc caacaatggc aaccttttga ccagcatgag ctgtcgcaga
     9301 gaagtcatag tcttgaacat tgacaccgtc caccagaatt tctcctgctg acacgtcgta
     9361 gaaacgtgga atcagattga ccagagttga tttaccagaa cctgttgacc caataaaggc
     9421 cactgtttga ccagtttctg ctttaaagct aacatgttca ataactgcct ccgaatttgc
     9481 cgcatagcgg aaggtcacat ccttaaactc gacctgacct ttgaagtttt catcagtcag
     9541 ctgcacttga acagggtttt ggatagaaga atgcaaatct aaaacttgat taatccgctt
     9601 agcagagacc atagttcggg gaagaacgat gaagagtgct cccatgagaa ggaagcccat
     9661 gacaacctac atggcataag acatgaaaac aatcatgtca ctaaagagag gcagacgcgc
     9721 tatcgaagca gcgtcgttaa tcacataggc cccaatccag taaatcgcta cactcaaacc
     9781 acttgaaatc cccatcatga taggattcaa aatagccata agacggttga caaacaaatt
     9841 caaacgggtc aattcatcat ttactgctgc aaatttttca ttttgataat cctctgcatt
     9901 gtaggcacga acgacacgaa tacctgttaa actctcacga gtgatactgt tcagtttatc
     9961 tgtcaacccc tgaatcaagg actgttttgg aaaggctagc gtcatcaaaa cggtcgtcat
    10021 caggacgttg acaatcacta ccacaagtac ggcccagagt cagtattctg aatgacctaa
    10081 aatcttccca atagcccaga tagccataat tggaccacgc gttaccactt gcaagcccat
    10141 agtaatcaac atttgaactt gagtaatgtc attggtagta cgcgtcaaga ggctaggaat
    10201 tgaaaatttc ttaatctctg tctgcgagta atccaaaact cggttaaaaa tatcacttct
    10261 cagcctacta gtataagaag ccgccactcg ggatgcaaaa aatccaactg caactacgga
    10321 caagaaggca agaaagagca ttcccatcat catgcttgcc ggctgccaca actcatctaa
    10381 attagtttct tgactaccta gcaaatccgt aattttcgag atataggtcg gcacttccaa
    10441 ctctagatag accgaaaagc aagtaaagag aatggctagt aaaatcatcc cccattcttt
    10501 tctactaatt cttttggtta atttctttat tctctcctcc tattcccttg atattttgcc
    10561 tgtagttgac cgagaacttt ctcaaaaatc agtaattcat cttcatcaat gtcttccatc
    10621 aactgcttgt ctatgcgttc aaaaaaagcc ttaacctgtt gcatctgaga acgtgctttg
    10681 tccgtcagac gaacaaactt agcccgctta tcaacaggac tcgcctccaa ttccaccaaa
    10741 ccattttgca ctatacgctt aaccagatta ctagcaacag acttggtaat attgagttcc
    10801 tgctcgatat ctttaatcaa gaccaagtct tggtttttct agcgattatc caaaaaacgc
    10861 acaacctgac cttgcggccc acccataaat tcaatgccgc aacgtttggc ttccttttgc
    10921 accatcaggt gaatttgatg accaaaacgc ttaaagacta acatcggttt atccataatc
    10981 tcccccttct aaataaaaat agttctctgg agaataatta aatttctatg agaactattt
    11041 tcttgattaa aaaaatccca agtgattttc tcacttagga tcatgttcta taggttaaat
    11101 taaaacccat ctacgttcgt ataaatcttt tggacgtctt cgtcgtcttc aagaacgctg
    11161 taaagttttt caaaggtttc aaggtcttcg cctgacaatt ccacttctga ctgaggaatc
    11221 atttccaatt cagtcacttg gaattcttca ataccagact cacggagagc aacgatagcc
    11281 ttgtgaaggt cagttggcgc tgtgtaaact gtgattgtac cttcttgtgc ttctacgtca
    11341 tccacatcca catccgcttc gagcaattgc tcaaagactg cgtctgcatc ttcacctcca
    11401 aatacaataa cacctttgtt gtcaaagagg taagaaacag aacctgaagc gcccatgttt
    11461 ccgccgtttt taccaaaggc tgcacggaca ttggctgctg tacggttgac gttagaagtc
    11521 aaagtatcca caattagcat agagccattt ggcccaaaac cttcgtaacg tccttctgta
    11581 aaggtttcgt ctgtgtttcc tttggcttta tcaatcgctt tatcgataat gtgttttggc
    11641 acttgggctt gtttagcacg gtcgataacg aatttcaaag ctgagtttga ttctggatct
    11701 ggatcacctt ttttagctgc tacatagatt tctacaccaa attttgcata taccttagag
    11761 ttagctccat ctttagccgt tttcttggct acgatattgg cccatttacg tcccattagg
    11821 aatctccttt tttcacattt taatctttat tattataaca caagtttttt tgattttcac
    11881 tagaggaaat ggattttatt agcaaatcaa gctaggatag cactttacct gctaagatgg
    11941 tcttaccttt ctatctttat caacagacac tcatccacat tcaaaaaaca aactagacca
    12001 ttatctgcaa atagaaagtt tcagccaagt ttgacaaagt cagctcaaat tactgtttga
    12061 agtttgtaga tataagcgac aaaaacaatc atactgcacc ttttgttgac agtctactcc
    12121 agacatatca tagttcaagt aaatactttg aaattcaaca gttcttatag gcgctattgt
    12181 attctaagaa atcaatagaa gagtttctaa gcaaacctct aatactcaat aaaaatcaaa
    12241 gagcaaacca gaaagctagc cgcaggttgc tcaaaacact gttttgaggt tgcggatggg
    12301 gctgacatgg tttgaagaga ttttcgaaga gtataattta cgtgttccca agatggagaa
    12361 gttagactag tacactggca cttctaaacc attgctagca attgatttgt tcatatttaa
    12421 tttcattttt tccataaatg ggtattagat ataaacagca aaatatttcc gatacgtgtc
    12481 gttcttgaat ttccaatcat ctaaaacaag taaaggataa tcaatcccct gtatatcaag
    12541 gaattggcta ccctttttac ttttttacac attctgtttg atagattcat tttaacatca
    12601 cgagcatact ccaatggaaa tcgctaggca agagataaac tttcagatat ccgcagagag
    12661 atcatcgcct ctttttgtcg caagcattct cctctcctag tcattttcta ccttatcttc
    12721 tacctgagga tagagagttg ttccccaaat agaaatcgtc cgcttacgca ctagtggcaa
    12781 atcggttttt tcataaaccg tacgccacca ttcccaggca agcccggtac actctctaat
    12841 tttgacagag agattacgaa cattcccttt taaaggaata ctagtggtaa agtgagccgt
    12901 taaatcctgc ccatttctgt cccaagcctt aggagtcaag acttccttac cttgatgatc
    12961 ataggataat tcattccaag taatataata ttgggcaaca taggcaccac tatgatccag
    13021 cagtaaatct ccgtttctgt aagctgtaac cttagtctca acatagtctg tactattttg
    13081 aaaggtcgca actacattgt cacgtaaaaa agaagttgta taggaaatcg gcaagcctgg
    13141 atgatctgct gtaaagcgac tgccttcttg aatcaagtcc tctaccatat ccaccttgcc
    13201 tgttacaact cgggcacccg aacttgggtc gccccctaaa ataaccgcct tcacttctgt
    13261 attgtccaaa atctgtttcc actctgtctg aggagctacc ttgactcctt ttatcaaagc
    13321 ttcaaaagca gcctctactt catcactctt actcgtggtt tccaacttga gatagacttg
    13381 gcgcccataa gcaacactcg aaatatagac caaaggacgc tctgcagaaa ttcctctctg
    13441 ttttaaatcc tctaccgtta cagtatcttg aaacacatct cctggatttt taacagcatc
    13501 tacgctgact gtataataaa tctgcttaaa attaacaatc tgaatctgct tttcgcccga
    13561 atggacagag ttaaaatcaa tatcaagaga attccctgtc ttttcaaagt cagaaccaaa
    13621 cttgaccttg agttgttcca tgctgtgagc cgtgattttt tcatactgca ttctagctgg
    13681 gacattattg acctgaccat aatcttgatg ccacttagcc aacaaatcgt ttaccgctcc
    13741 gcgaacactt gaattgctgg ggtcttccac ttggagaaag ctatcgctac ttgccaaacc
    13801 aggcaaatca atactataag tcatcggagc acgatcgacc gcaagaagag tgggattatt
    13861 ctctaacaag gtctcatcca ctacgagaag tgctccagga tagaggcgac tgtcgttggt
    13921 agctgttaca gaaatatcac ttgtatttgt cgacaagctc cgcttctttc tttcgataac
    13981 aacaaactca tcgggtagct gattaccctc tttgatgaaa cgattttcaa tactttctcc
    14041 ctgatgggtc aagagtttct ttttatcgta attcatagct agtataaagt catttactgc
    14101 tttatttgcc atcttctacc tcctaataag ttcctggatt gagttgcata aactcagact
    14161 tgttcagcga aatcagccgt ggttggacta agtaatccaa aatttcctcg tacaattctt
    14221 ctgagacatt gcgtcgccgt ctggctagat aagaagtcgg aatgcccgta ttatccaaca
    14281 taaatacctt atctaagtca atcaaggttg gtcttgtaaa aggattacga gctagatccg
    14341 gctcttctat cataaagttc ttgaccaaac gtctggtcaa gagagctggt ttgaaggtct
    14401 gatttttaac caactctttg tttttagtca tgctgttgtc aatacagata tacatatgat
    14461 tcttcacagc caaatcgcta ctaatagtcg gaaaaggcaa ataaagagct acaacatctc
    14521 ctctcttaat caagcaagag cacccccttt tctcctaatg taacatagac aggattgacc
    14581 aagtcttctg attgactcag aatttccaaa gtttgagttt ggcgcgctgt caatttagta
    14641 gcatcttgtc tcttcaatac aaaatgcttg tcgccaataa ccttgacaat ataatccttc
    14701 tccaaagctg actggtaaat ccacatcaga tgttgtctgt cctgagaact caagagagaa
    14761 ggattttcaa gcctcccgat agtctgataa aaatcaaaaa caggagctaa ctcctgccaa
    14821 tctgattggc tagttgtcaa ggctagaaaa agggctttgc gagctgatac ttcttggtta
    14881 gccttgagag ttactttccc ctccaagttt tttagaaatc gggaaactcc agaaagcaaa
    14941 tttttctcta actgcgagaa ataaaaacct ttcgttccca gacataagtc tttcatgtcg
    15001 ctttctctag caaataagag ctcaaacatt tgatagtaaa agaaaaatat ctggcactgg
    15061 gtcgcgctca tcttttcctt atcggcttct ttttttaacc agagcaaggg cgacaggtag
    15121 ctggattgag acatttcctc tacctcctac tcttttttaa ctggagcatc tgcactagct
    15181 gccacttctt ttgactggat actttcccac tggttaatct cctctgagat aagaccttcg
    15241 catgtcttga caaatagggc aaaagccttg gtctttcctg catatttctc cgtttggcat
    15301 tgatagagga atttttcttt ctccaggagt tgcgcagttt tttggtaaga aatccaattt
    15361 tcctttgcat tatacaaatt gataatcccc tcacacagca agccgagact ggataaggca
    15421 accgaaatca aacggtagcg atcacctggc ataggaatag cacaaaagac agctatgagg
    15481 aaacctgcca cgatttctgt tatttttaat accttatagc gtctacgatg ttgaacgctt
    15541 ttctttaaaa aatgagctat ctgtacgtct aatcgctctg tcaggtacat ttcttctggc
    15601 gtcatattcg taactccttt catttacttt gataaccata gtataacata tttgaatatc
    15661 aaagtatagt aaaaactgga ctataactta ttag
//
LOCUS       DAVMGZ010000033        15459 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000033 DAVMGZ010000000
VERSION     DAVMGZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15459
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.034"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(101..451)
                     /locus_tag="VYQ93_000885"
     CDS             complement(101..451)
                     /locus_tag="VYQ93_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335598.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(591..926)
                     /locus_tag="VYQ93_000886"
     CDS             complement(591..926)
                     /locus_tag="VYQ93_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335599.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(1017..2327)
                     /gene="der"
                     /locus_tag="VYQ93_000887"
     CDS             complement(1017..2327)
                     /gene="der"
                     /locus_tag="VYQ93_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV3335600.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDTIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(2341..3054)
                     /locus_tag="VYQ93_000888"
     CDS             complement(2341..3054)
                     /locus_tag="VYQ93_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV3335601.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(3051..3947)
                     /gene="dnaI"
                     /locus_tag="VYQ93_000889"
     CDS             complement(3051..3947)
                     /gene="dnaI"
                     /locus_tag="VYQ93_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV3335602.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAIGDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(3948..5117)
                     /locus_tag="VYQ93_000890"
     CDS             complement(3948..5117)
                     /locus_tag="VYQ93_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV3335603.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPDLAELGSQEDLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQII
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(5118..5591)
                     /gene="nrdR"
                     /locus_tag="VYQ93_000891"
     CDS             complement(5118..5591)
                     /gene="nrdR"
                     /locus_tag="VYQ93_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV3335604.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5731..6096
                     /locus_tag="VYQ93_000892"
     CDS             5731..6096
                     /locus_tag="VYQ93_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV3335605.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            6101..6796
                     /locus_tag="VYQ93_000893"
     CDS             6101..6796
                     /locus_tag="VYQ93_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3335606.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6813..7580
                     /locus_tag="VYQ93_000894"
     CDS             6813..7580
                     /locus_tag="VYQ93_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335607.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(7992..9191)
                     /locus_tag="VYQ93_000895"
     CDS             complement(7992..9191)
                     /locus_tag="VYQ93_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3335608.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(9188..10081)
                     /locus_tag="VYQ93_000896"
     CDS             complement(9188..10081)
                     /locus_tag="VYQ93_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3335609.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQDQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            10197..10277
                     /locus_tag="VYQ93_000897"
                     /pseudo
     CDS             10197..10277
                     /locus_tag="VYQ93_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10762..10975
                     /locus_tag="VYQ93_000898"
                     /pseudo
     CDS             10762..10975
                     /locus_tag="VYQ93_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            11390..12007
                     /locus_tag="VYQ93_000899"
     CDS             11390..12007
                     /locus_tag="VYQ93_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV3335610.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(12327..13214)
                     /locus_tag="VYQ93_000900"
     CDS             complement(12327..13214)
                     /locus_tag="VYQ93_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV3335611.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVVRHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGENIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(13363..15246)
                     /locus_tag="VYQ93_000901"
     CDS             complement(13363..15246)
                     /locus_tag="VYQ93_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV3335612.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     SQGNKVKAGDVLGKFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVSKGDAVIE
                     VKI"
BASE COUNT         4554 a         3386 c         2865 g         4654 t
ORIGIN      
        1 aatccactat acaagatgaa tatttctctc aagtttcata atcaaaataa aaatgactgt
       61 atctatagct ccataaacaa atcatagata cagtcatttt ttagttccta caccttcctt
      121 tatgcacacc tttagatttc cctagttccc gtctcaagtc aagcattttt ttgaaacaga
      181 tatagtctaa ccagtcacta gcaaaagcga taagtagtaa taatagccaa ataccccata
      241 acttgatatc tgtcaaattt ctcaagacga tattgaaaaa cagaactgaa aaaactgtcc
      301 aagcaaggct aaaaagagca tagagggtga tgtaaatcca gtaaaaatag taaaaaattg
      361 ggaaaaattt actttctcta ttatccttgt caatccagct gaaaaagtaa aaacagggta
      421 ataagattat cattttaaac aagtgttcca tcacaagcct ccttttttaa attgttttct
      481 ttagtttcgt tctattaaat gttattacca atataaacct tggaatagcg tgtaataaat
      541 gattttagag gtaccataag ccacctccta caaatagaaa ccgatataaa tcaatgcctt
      601 ccacccttag acttccctag ttcctgtctc aagcgaaaca tttctttgaa acaggaataa
      661 gttaaccaat tcataccaat agctagcaga ataaaaagaa accaaatgcc ccataacttg
      721 atatctgtca catttctcaa gacggtattg aaaaacagaa ctgaaacaac tgtccaagca
      781 aggctaaaaa gagaatagaa ggggatgtaa aaccagtaaa aataataaaa aattggaaaa
      841 aacttactat ttctgttggc cttctcaatc cagttatcaa aataaaagta cggtgctaaa
      901 agtaagaatt taaacaaatg ttccatcacc gacatcaccc tccttctttt gatagcgttt
      961 tctattattt tattatatca aaaaaatccg gaactgtcat tccagattct acttttttat
     1021 ttgcgttttc ttgcgatgag atgaatcggt gttccctcaa aaacaaaggc cttgcggatt
     1081 tgattttcca agaaacgcag gtaagaaaag tgcatgagtt cttcttcatt gacaaagatg
     1141 acaaaggttg gtggtttggt tgccacttgg gtcgcataga aaatcttgag acgttttcct
     1201 ttgtctgtcg gtgttgggtt gatggcaatg gtatccatga tgacatcgtt caagacagct
     1261 gatggaatac gtgtattttg actttcgctg atttgcttaa tcatctcagg aagtttgtgg
     1321 agacgttgct tggttaaagc tgatacaaag ataatcggtg cgtaaggcag gtattggaac
     1381 tgctcacgga tatcttcttc ccagtttttc atagtgtggt tatctttttc aagcgtatcc
     1441 cacttgttga ccacgataat catcccttta ccagcttcat gggcaaatcc tgcgatacgc
     1501 ttgtcgtact cacgaatgcc ttcttccgca ttgatgacca tcaagaccac atctgaacgg
     1561 tcaatagcac gcatggcacg cataacagag tatttctcag tattttcata aaccttacca
     1621 gacttacgca taccagccgt atcaatcatg gtaaactctt gaccatctgt atctgtaaag
     1681 tgggtatcaa tggcatcacg agttgttcca gcaacaggac tagcaataac acggtcttcg
     1741 cccaagatag cattgatcaa gcttgatttt ccaacgtttg gacgaccaat caggctaaac
     1801 ttaatgacat ctggattttc ttcttcatat tcatttggaa ggttttccac aatcgcatct
     1861 agtacatccc ctgtaccgat accatggaca gatgagatag gcagtggttc acccaaaccg
     1921 agggcataga aatcatagat atcatttcgc atctcagggt tgtccacctt gttaactgcg
     1981 aggataactg gtttgtgggt cttataaagc ttacgggcta cgtattcgtc tgcatcggta
     2041 attccttcct taccggacac gacaaagacg ataacatctg cttcttccat ggcaatttct
     2101 gcctggtgct tgatttgttc catgaaagga gcatcgacat catcaattcc tcctgtatca
     2161 atcatgctaa aagaacgatt gagccactca cccgttgcat aaatacggtc acgtgtcact
     2221 ccttcgacat cttctacaat ggagattcgc tcaccagcga tccgattaaa tagggttgat
     2281 ttcccaacat tgggacgtcc tacaatggca atagttggta gggccataat ttctcacttt
     2341 ctacaataat ttcttctgtt caagattttt tctagttgag cttggttcag cttgaccaaa
     2401 ctgttctgct aggcgctgac tccagcttgt ggtcgcacgc gccccagcat agtcagcctg
     2461 aacacggtca taagcttgga ttgcctcagt tgactgttct tggtattctt cctcaaagac
     2521 aacattctct agtggcagtc tcggtttcat atcatgatgt tgatttggca cacccagtgc
     2581 catcccaaag acagaatagg tgtagtcagg taggttaaag agctctgcca cttcttcaga
     2641 cttgtatcga accaaaccga taatcacacc accatagccc aagctttcag ctgccaacaa
     2701 ggcgttttgt ccagcaagag ctgcatcgac cgaactaatc aagagacctt ccacaccttg
     2761 gggttggaag gtgtcggtat gaagtcgggc tcccttttct gctcggttca aatctccgac
     2821 aaagagaagg aaaacagcag actggcgaat ggcttcttga ggtaccaatt catacaaggc
     2881 atctttcttc tcttgacttc gtaccacaat cacagagtag gattggaaat tcttccaaga
     2941 tgatgccatc tgggctgctg tcaaaatctc atttaagtct acttggggaa tttcttgctc
     3001 tttaaacctg cgcactgaag tatgagcctt catcaattta atggtttctg tcatcgacgg
     3061 tttactcctt ctaaacgagt ctcctcagcc aaataacgga tgcgttccat gacccgtctg
     3121 gcttcccagg tttcgtcatt tccatgtttc actttcgcaa aatgcttctc caaatcttca
     3181 aagttgaagt tggatgtgaa aaaggtcggt aaattttcct gcatccgata ttggagaatg
     3241 acctgcagga tttcgtcacg cacccaagcg gtggattgct cggcaccaat atcatctaaa
     3301 atcaggactt cagaaagctt aatctcatcc actaaggtct taacattgcc atcaccgata
     3361 gcatttttga catcaatgac aaagctagga tagtggagga gagttgatga aacaccacgt
     3421 ttttctgata aatcatgagc taaggccgcc accatgaaac ttttacccac accaaagtct
     3481 ccatataagt aaagaccttt tcgaatagct ggatattgct ccacgaaggc tagtagcttt
     3541 tcaaaaactg gtaagcgccc caaatcatcc aagtcaactt gagccaaact agctttcttg
     3601 agactggctg gtagattgat taacttgaga cggttcttaa tagccgcttc tttttcagcc
     3661 gcgattagct caggagtttc ttcatatgaa acatctgcat agccatgatt cttaaccaaa
     3721 atcggcttgt agcctttggc aatataatcc gtatccccac ggagaaactt gtcacgctcg
     3781 gtgatgtact gattaaactt ggagatactg cgatttaatt cctctggagt caaggattct
     3841 tgctggataa aagccgcaac atcagggtcc ttcatgattt tctggaccaa atcttgataa
     3901 taaaaacggc tgggttgacg tttgagtacg tctccgacac tttccatcta atctcctcct
     3961 ttttctaatc gagctaatag ttcttgcttc ttacgttcta gttccagacg agtttcctcg
     4021 ctggtttcat tcttatattc aggattactc catttaggaa cattggtttt tactggggca
     4081 gtctgattct gtttttgtgt ttttgctttc tgtcctcgct cacggatgcg caagactgcc
     4141 ttttctgccg aatgaatctt ttgataggca tagtcattgg ctaccttcat ggcatatttc
     4201 tcattgatat ttgccgaatc gaccttatta aaggtcaata aaagaatgat attgatgact
     4261 tcgtccagca agcccaagcc agccatctgt tgcaagagtt ctctttctgt ttgggtaatg
     4321 gttcccttgc gtgtttgctt gatttctgcc aagaactgca gggcagtttt acttttggct
     4381 tctttgataa tggtcgcttc cttaagacta aagtcagagg aaactggttt ttgagcaatt
     4441 ttttcacgca tgcgtttggt tgaaataatc tgagaaacag ctgttgactt ggccaattga
     4501 taggtttcaa accaagtcca tttcttctcc tcggcaatag caaagaggtt taagacatcg
     4561 gactgctcat ccgcaaagcg aagtccatct cgagccataa gctgacgaaa atggtccaag
     4621 tcaaaatcgt tggccacttt cttcttgaga ccaaggtctt cttgactgcc tagttctgcc
     4681 aagtctggaa agacttgatt gagtgagaca ggtatttctt caccatcagc actttcaact
     4741 ttcaaatcct ccacagctac atcgccaatc tttttctcta agagtctgcg ataaacagga
     4801 tgccccaaga agtcttgact agatagagga gcatggaggg ctagctgata aacatccccc
     4861 ttttgataga gagtcaagag attaaaagca gataagattt tcaatgattt tatcagtcta
     4921 tccatcccaa agttgagatg attgaggata cttgaaaaaa gatattcctt tctaccatta
     4981 tcccaaaaac taatcgtata aagataaagg ctcagtgcct cctgaccgat aatcgggagg
     5041 tagcactgta ccagagatga ggtatcttgc gacacccgat tattctttag ataagaaaaa
     5101 cggtcaattg gcttcattta tctttccttt ttctttttag aggactgggt gatttgttgg
     5161 agcaagctct ctaactcact gacatcctta aaactacgat agacactagc aaaacgtaca
     5221 taggtaatct cgtccaattc agccaactcc tccatgacga gtgaaccaat gtcctcactt
     5281 tgaatttcat tttcatttcg accacggagt ttctgttcga tacgattgac taccatgttg
     5341 atttcatcac ttgacacagg acgtttctgg gctgagcgga taatcccatt aaagatttta
     5401 tctctggaga attgttcccg tgtgccatct tttttaacaa ccactaaggt tctttcttct
     5461 actcgttcgt aggttgtaaa acggtgttgg cattcgtcgc actcacgtct tctacgaatg
     5521 gtgttccctt cttctgcttg gcgactatcg ataacacttg acttggtagc cccacatttt
     5581 ggacaacgca tcctttccct ccttatcgtt ttcttttcat tataccattt tttaagcgat
     5641 tcccaaaaca attcttcttt ttgcttgaca agttttttgt tttgttgtat tatttagtta
     5701 agacaaaagg ataagagaaa ggagactaag atgtcctgga catttgacaa caaaaaaccc
     5761 atctatttac agattatgga gaaaatcaag cttcagattg tttcccatac actggaaccc
     5821 aatcaacaac ttccaaccgt gagggagcta gctagcgagg ctggtgtcaa tcccaatacc
     5881 atccaaagag ccttatcaga ccttgaacga gaaggatttg tctacagcaa gcgaacaact
     5941 ggacgatttg tgactaagga taaggagcta atcgctcagt cacgcaaaca attatcagaa
     6001 gaagaattgg aacacttcgt ttcctccatg atccattttg gctatgaaaa agaagaacta
     6061 ccaggcgtag tcagtgatta tattaaagga gtttaagcct atgtcattac tagtatttga
     6121 aaatgtatcc aaatcatatg gagcaacacc agcccttgaa aatgtttctc ttgacattcc
     6181 agctggaaaa attgtcggcc ttcttgggcc aaacggctca ggaaaaacaa ccctgattaa
     6241 actaattaat ggcctcttac aaccagatca aggacgtgtc ctcatcaacg acatggaccc
     6301 aagcccagca accaaggccg ttgtagctta tttgcctgat acgacctatc tcaatgagca
     6361 aatgaaggtc aaagaagccc taacctactt caagaccttc tataaagatt tcaatcttga
     6421 acgcgctcat catctacttg cagacctggg cattgatgaa aatagtcgtc tcaagaaact
     6481 atcaaaagga aacaaagaaa aggttcaact gattttggtt atgagccgtg atgctcgtct
     6541 ctatgttttg gatgaaccca ttggtggggt ggatccagca gcccgtgctt atatcctcaa
     6601 taccattatc aacaactact caccaacttc taccgttttg atttctaccc acttgatttc
     6661 tgatatcgag ccaatcttgg atgaaattgt cttcctaaaa gacggaaaag tcgtccgtca
     6721 aggaaatgta gatgatattc gctacgagtc aggtgaatcc attgaccaac tcttccgtca
     6781 ggaatttaag gcctaagcaa aggagattat ttatgttttg gaatttagtt cactacgaat
     6841 ttaaaaatgt taacaagtgg tatttagccc tctacgcagc cgtgctagtc ctttctgccc
     6901 tcatcggaat acagacacaa ggctttaaaa atctacctta ccaagaaagt caggctacta
     6961 tgctactttt tctagctaca gtctttggtg gcttgatgct tacacttgcg atttcaacca
     7021 ttttcttgat tattaaacgc ttcaaaggta gtgtctacga ccgacaaggc tatctgactt
     7081 tgaccttgcc agtttctgaa caccatatca tcacagccaa actaatcggt gcctttatct
     7141 ggtcattgat tagcaccgct gtattggctc taagtgctgt tattattctg gctttaacag
     7201 ctccagaatg gattcctctt tcttatgtga ttacatttgt agaaacacat ctccctcaga
     7261 tctttcttac aggtatatcc ttcctactaa ataccatttc aggaatcctc tgcatctacc
     7321 tggctatttc cattggacag cttttcaatg aataccgtac agcactcgct gttgcagcct
     7381 acattggtat ccaaatcgtc attggattta ttgaactttt cttcaatctt agttctaatt
     7441 tctatgtcaa ttcactggta ggactcaatg accatttcta tatgggagca ggtatagcca
     7501 ttgttaaaga actcatattc atagctatct tttatctcgg aacctactac atcttgagaa
     7561 ataaggttaa tttgctttaa ataattttta cctagatatg taacatactc atagaacaaa
     7621 agagaccagg caaaaagtct ttaaaattag aaaacgcata gtatcaagtg ttgaatatga
     7681 actgccccca aaagttagac agaaaaaatc taacttttgg ggggcagttc aaaaaatcct
     7741 tgatattatg cgttttttgt gagctgactt atttcctttc actatatcgc aaaatgaaat
     7801 aagaacggaa cgatgggatt ttggaattca aatcaattta taagaatgtt ttagaagtaa
     7861 tattatccta ttccagattc agttcactat acaattgagt tttcaagcaa cctgtttaca
     7921 taatgtgtac ataattaggt tcgtgattcc acccttttca cctttaaaaa cctcgctttc
     7981 gcaaggctct tctatttata agataaggca cgtttaaagg ttttccaaat ccctaaatca
     8041 tccgtttgaa gaacgagact agcatacatg cgtccgataa atcctgttgc taccaccgca
     8101 aaaatcactg taatagcaag tgaaatccat gcttctgctc cccccgcata gccattaatc
     8161 gttcgaaacg gcataaagaa ggtcgaaata aagggaatat aagaaccaat cttcaagagg
     8221 agattgtcac cagctgcacc tagagctgtc actccaaaaa aaccacccat aatcaaaatc
     8281 atcaaaggcg acaaggcttt ccctgagtcc tcaggacgag aaaccataga tcctaggaag
     8341 gctgccaaga ctacatacat gaaaagactg atcaaaataa agagcaaggt attcagtgag
     8401 atagcatctc ccaagtgatc caaaatacca gactgagcca agaatggcaa atctttaaag
     8461 agcaaaacgg cagccagacc acctacaaca tagatcccaa tatgcgttaa aatcactaga
     8521 aacagagcca tcatccgcgc atagaaatag tgacttgccc ttatgctaga aaaaacgact
     8581 tccataattt tggtgccttt ttcactggca acttcctgag ccgttacacc cgcataggta
     8641 atcagaatca tataaagaaa gaatcctaag gcacctgctg caattgtttg aataaacttt
     8701 ttattttcct tggcttcatc aatcttttct gtgaattgaa ttgtctgcgc taagcgtttt
     8761 tcctgctctt gagacaagga agcagttgaa cgattaagct gattttgcag ttcattgagt
     8821 gtacctgtaa cctcaaattt aattccattt tcaagcgatg tttcgccatg ataaactgcc
     8881 tttagaacac tatcttcttg atcaatggtc aaataacctt ttaatttttc ttctttaatt
     8941 gcttctttgg cacttgcttc atctttatag tcgaagttaa caccatttac attcttcagt
     9001 ccttctgcta cagatggcac tgttgtcact actgccactt tattattttt agccatagaa
     9061 gaaccttgga gatgcccaat tcctacagag attcctaaaa agaggaacgg cgaaatcacc
     9121 ataaagaaga aactccatga ctcgacatgt cgaagatagg tttccttgat tacaacccac
     9181 atatttctca tacttccact cctgattcta gtttaaagat ttcatcgata gttggcgctt
     9241 gttggtcaaa tgttgcgata tattgatctt gagtcaagat tgggaagagt tcccttccag
     9301 cgctctcatc ctccaaaatc aatttccaac tgccttgttt ggtcaagctc acctgtttga
     9361 catgaggaag attttccaat tcttccttgc ttcgttcact tgaaacaaag agacgcgttt
     9421 tcccgtattg attgcggaca tcctgaactg gtccgtgcaa gaccacacgg ccatctcgga
     9481 tcatcagaat atcgtcacaa agttcctcaa cattggtcat gacatggtca gaaaagataa
     9541 tggttgctcc gcgctctttt tcctgaaaaa tgacttgttt gagcaattct gtattaactg
     9601 ggtccaatcc actaaaaggc tcatccaaga taatcaggtc tggttcatga atcagagtaa
     9661 taatgagctg aatcttctgc tgatttcctt ttgacagact cttgatttta tctgtcagct
     9721 ttcctttcac ttccaacctc ttcatccatt gagggagttt ttctttgact tctttggcat
     9781 ccatgccttt tagagtcgcc aagtagcgaa cttgttcaag aactgtcaat ttaggcatga
     9841 gactgcgttc ttcaggcaga taaccaatcc gagcataggt ctcctgacga atatcctgac
     9901 catccagacc gatttctccc tgatattcta ggaattttaa aatactatgg aaaatcgttg
     9961 tttttccagc accatttttt ccgactagtc ccaaaatacg acctggtcgc gcttgaaagt
    10021 caataccaaa caaaacttgc ttggatccaa aacttttctc tagacttctt acttctagca
    10081 tctttcacct ccgaaatttc ttgcactcat tatactcctt tttgatagcc tttacaatgt
    10141 ttttgtccat ttttagaaga ctattgctgt gtaaaatatg gcctggagca cttttatact
    10201 caatgaaaag caaagagcaa actaggaagc tagccgtagg ctgctcaaag tacagctttg
    10261 aggttgcaga taaaactgac gaagtcagct caaaacactg ttttgaggtt gtggatagaa
    10321 ctgacgaagt cagtaactat atctacggca aggcgaagct gacgtggttt gaagagattt
    10381 tcgaagagta ttagtgataa atccattata cagtagcaaa cttaatttat accttccgct
    10441 cctcaactgt ctatttttaa tcctgaattg ttatttgagt aactcctttt tcctcataaa
    10501 gttttcttcc tctaaaactt ctggaaaaaa gcgaatagtt tcagacaaca tttttataag
    10561 aaacaagttc atctgtcatt tcaagaagga gtaatccttt atctactaat ggacggaacg
    10621 gaattcaacc gcttgtccga tatgttttct aaggattata tagtaaaatg aaataaaaac
    10681 aggacaaata gatcgggaca gtcaaatcga tttctaacaa tgttttagaa gtagatgcgt
    10741 actattctag tttcaatcta ttatatctat tatgcacacc actataggat ctaataaaaa
    10801 tcacaacaag ttcattcata gatggttacc tcagggaact aagaaaacga ctacaaagga
    10861 agtcgcattc atcgaaaagt ggattaacaa ctatcctaaa aaatgcttga actacaagtc
    10921 ccccagagaa gacttctgga tgactaactt gaacttgaaa tttagcaata attaattcac
    10981 catctaacta tatttagtaa ttatttcaga actgattaat attaaaatta actaacaatt
    11041 caaaggattc atactagcca taaattacgt ccatcagaga gagactctta ctacttttag
    11101 attttagtct ttctagcttc agaatacatc taaactttag ggaaaatgac tattcgaaag
    11161 cgcgaatgcc tcaaaattat ctcagataag ctattcgaaa cttagaatgc ttttaaattt
    11221 atggaattgc gattattcga aacctagaat gcatataacc tttagttgac agacctattc
    11281 taagtctcga agggctattt actttctatt ccttatcaaa aaagactcat tccccctttc
    11341 tcctccaaaa tatggtatag tagaaatata ctatctatga ggagtttaca tgtcacagga
    11401 taaacaaatg aaagctgttt ctccccttct gcagcgagtt atcaatatct catcgattgt
    11461 cggtggggtt gggagtttga ttttctgtat ttgggcttat caggctggga ttttacaatc
    11521 caaggaaacc ctctctgcct ttatccagca ggcaggcatc tggggtccac ctctctttat
    11581 ctttttacag attttacaga ctgtcgtccc tatcattcca ggggccttga cctcggtggc
    11641 tggggtcttt atctacgggc acatcatcgg gactatctac aactatatcg gcatcgtgat
    11701 tggctgtgcc attatctttt atctagtgcg cctatacgga gctgcctttg tccagtctgt
    11761 cgtcagcaag cgcacctacg acaagtacat cggctggcta gataagggca atcgttttga
    11821 ccgcttcttt atttttatga tgatttggcc cattagccca gctgactttc tctgtatgct
    11881 ggctgccctg accaagatga gtttcaagcg ctacatgacc atcatcattc tgaccaaacc
    11941 ctttaccctc gtggtttata cctacggtct gacctatatt attgactttt tctggcaaat
    12001 gctttgacac gtaaaaaatc cgtttggttt cccaagtgga tttttaaagc gtagattaac
    12061 tatagcttga tactaaatat actttggtat ggaaatcatg catatttttc gatagtgagg
    12121 cgaggactta cctagccttc ccgccgtgat agaaacacct gaaatctaat ggtttcaggt
    12181 attcggaaac tttgagccta gtgtctcaaa gtttaggtat ggaatttcga agaaagtcgc
    12241 taccgtccgt aatcacttaa ggaaaggctc aaaaatattg ttttcaacca caaaatccgt
    12301 ttggtttccc aagcggattt tgtgctttat tttgaaactt cttttgcaag aacaaagttc
    12361 ccaagtgtgg cagaaccatt tcctgcgact gctggcgtca cgatatagtc acgcacatct
    12421 ggtactggta ggtaaccatt aagaagagat gtaaatttct cacggacacg gtccagcata
    12481 tgttgttgag ccatgacccc tccaccaaag acaatcacgt ctgggcggaa agtcactgtc
    12541 gcattaaccg cagcttgagc gatatagtag gcttgaacat cccaaacagg gttgttgagt
    12601 tcaatatttt ccccacgtac acctgtacga gcttccaaac ttggaccagc tgcatagcct
    12661 tccagacatc ccttatggaa aggacaaaca cccttaaact ctttttcaat atccattggg
    12721 tgtctaacaa cataataatg acccatttca gggtgaccca caccaccgat aaactcacca
    12781 cgttggatga cacctgcacc gatacctgta ccgattgtgt agtaaaccaa gttttcgata
    12841 cgaccaccag cattgttacg ggcaaccact tcaccgtaag cagagctgtt tacgtctgtt
    12901 gtgaagtaca ttggcacgtt tagggcgcga cgaagggcac caagcaagtc cacatttgcc
    12961 cagtttggtt ttggagtcgt cgtgataaag ccataagttt ttgagttttt gtcaatatca
    13021 atcggcccaa atgaaccaac tgcaagacca gcaaggttat cgaattttga gaagaactca
    13081 atggttttat cgattgtttc gattggagtt gttgttggaa attgtgtttt ttctacaacg
    13141 ttaaagtttt catcaccgac agcacagaca aactttgtac cgcccgcttc caagcttcca
    13201 tataattttg tcatgataaa cctcttgttt ttattttctt tattatagca tacttcgaaa
    13261 gtctaaatgt ctctattttt tagattttcc tctgtaaatc ttactatcta ataaaaacga
    13321 acaaacatgt catttgttcg ttttcacatt agagaggatt gattagattt tcacttcgat
    13381 cacagcatcc cccttcgaaa ctgaacctgt tgcgactgga gctactgaag cgtagtcacc
    13441 tgtatttgta acgataacca ttgttgtatc atcaagtcca gctgcagcga ttttgtttga
    13501 gtcaaatttt cccagaacat cgccagcttt caccttatta ccttgagaaa cttttgcttc
    13561 aaaaccgtca ccgttcatag atacagtgtc aataccaacg tgaatcaaaa cttcagcacc
    13621 atttcttgtt ttcaaaccaa aagcgtgccc tgttggaaag gcaattgaaa cttcagcatc
    13681 agctggtgca tagaccacgc cttggcttgg tttcacagcg ataccttgtc ccatagctcc
    13741 acttgagaag actgggtcat tgacatcagc aagagcgaca acatcaccga cgataggagt
    13801 tacaagtgtt tcattttgaa gagctgctgg cgcaacttct tctttttctt cagccacttc
    13861 agcttgtttt gcagctgcag ttgcgtctac ttcatcttcg taaccaaaca tgtaagtaag
    13921 agcaaaacca agggcaaatg atacagctac cataagaagg tattgtggaa gttgtccgtt
    13981 acccacataa agcattgtac cagggatgat ggtgatacca ttaccagtac cagcaagtcc
    14041 aaggatagaa gccaatccac caccgattgc accagcaatc aatgaaagga agaatggttt
    14101 acggaagcgc aagttcaccc cgaagatagc aggctctgta atacctagga aggcagaaag
    14161 agcagccggg aaagcaagtg ttttcagttt tggatttttt gttttaacac caaccgcaac
    14221 agtagcagca ccttgagctg tcatagcagc tgtgatgata gcgttgaatg ggttagcatg
    14281 gtcagcagca agtaattgca cttcaagcaa gttgaagatg tggtgcacac ctgacacgac
    14341 gatcaattgg tgaaccccac caatcaagaa accaccaaga ccaaatggca tgctaagaat
    14401 cgcttttgta gcaataagga tgtagttttc aacaacgtgg aaaactggtc caatgacaaa
    14461 gagtccaagg atagacatga ccaaaagtgt cacgaatggt gttaccaaga ggtcaatgac
    14521 atctggaaca accttgcgga cagctttttc aaatttagct ccgacaaccc cgatgatgaa
    14581 ggctggaaga acagaacctt gcaaaccaac aacagggatg aaaccaaaga agttcatcgc
    14641 tgttacttca ccaccttgag caactgccca agcgtttgga agtgagccag agacaagcat
    14701 cataccaaga acgataccaa cggcaggatt tccaccaaat acacggaagg ttgaccacac
    14761 aaccaaacct ggcaagatga tgaaggctgt atctgtcaag atttgtgtgt aagttgcaaa
    14821 gtcacctgga agtggcattt caagagcgtt gaaaagacca cgcacaccca tgaagagacc
    14881 tgtcgctacg ataactggga tgattggaac gaaaacatca ccgaaagtac ggatagcacg
    14941 ttggaaccag ttcccttgtt tagcaacttc tgctttcatg tcatccttag atgatgttgg
    15001 taagccaagt acaacaactt catcgtacat tttgttaact gtacctgtac caaagataat
    15061 ttggtattgc cctgagttaa agaaagcacc ttgaactttt tccaagttct caatcacttc
    15121 tttattgatt ttctcttcat ctttgaccat gacacgtaga cgagtcgcac agtgggcaac
    15181 actattgaca ttttcacgtc cgcccaaggc atcgatgact ttttttgcaa tttcctgatt
    15241 gttcatttgc aaaaatctcc ttatataaca ttttgttctt gtttgaaagc gattttattc
    15301 gcccttacga ctattatttt atcatgtttt aaaaatatgt caagcgtttt gcagaaaaaa
    15361 tattctatct acttagtggg caagctttag attgattgtt tttgactgtt tcatcaggaa
    15421 tttcttgaaa aataagtttg aaaccttggt taaaaagtc
//
LOCUS       DAVMGZ010000034        15418 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000034 DAVMGZ010000000
VERSION     DAVMGZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.035"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            51..191
                     /locus_tag="VYQ93_000902"
     CDS             51..191
                     /locus_tag="VYQ93_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blpT protein"
                     /protein_id="HEV3335613.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQVTADDIRLQELLNTTMQELKKQ
                     KS"
     gene            606..2165
                     /locus_tag="VYQ93_000903"
     CDS             606..2165
                     /locus_tag="VYQ93_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV3335614.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(2314..3213)
                     /gene="htpX"
                     /locus_tag="VYQ93_000904"
     CDS             complement(2314..3213)
                     /gene="htpX"
                     /locus_tag="VYQ93_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV3335615.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSTLYINDPKKG
                     GGFQKLFYTHPPISERIERLKHM"
     gene            complement(3215..3775)
                     /locus_tag="VYQ93_000905"
     CDS             complement(3215..3775)
                     /locus_tag="VYQ93_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV3335616.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            3869..4582
                     /gene="rsmG"
                     /locus_tag="VYQ93_000906"
     CDS             3869..4582
                     /gene="rsmG"
                     /locus_tag="VYQ93_000906"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV3335617.1"
                     /translation="MKPETFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            4885..6168
                     /locus_tag="VYQ93_000907"
     CDS             4885..6168
                     /locus_tag="VYQ93_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV3335618.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFIPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6363..7934)
                     /gene="ffh"
                     /locus_tag="VYQ93_000908"
     CDS             complement(6363..7934)
                     /gene="ffh"
                     /locus_tag="VYQ93_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV3335619.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(7946..8278)
                     /locus_tag="VYQ93_000909"
     CDS             complement(7946..8278)
                     /locus_tag="VYQ93_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV3335620.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(8369..8746)
                     /locus_tag="VYQ93_000910"
     CDS             complement(8369..8746)
                     /locus_tag="VYQ93_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV3335621.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKRDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYILTPMGMQEFTIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            8818..10122
                     /locus_tag="VYQ93_000911"
     CDS             8818..10122
                     /locus_tag="VYQ93_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV3335622.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMIAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQSPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPKDKDFFARTSPHLLPFFEKNVTLSD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLYLIENDHFTPNKN"
     gene            10135..10941
                     /gene="yidA"
                     /locus_tag="VYQ93_000912"
     CDS             10135..10941
                     /gene="yidA"
                     /locus_tag="VYQ93_000912"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV3335623.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(10997..11068)
                     /locus_tag="VYQ93_000913"
     tRNA            complement(10997..11068)
                     /locus_tag="VYQ93_000913"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11033..11035),aa:Arg,seq:cct)
     gene            complement(11111..11458)
                     /gene="rplS"
                     /locus_tag="VYQ93_000914"
     CDS             complement(11111..11458)
                     /gene="rplS"
                     /locus_tag="VYQ93_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV3335624.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(11577..11906)
                     /gene="crcB"
                     /locus_tag="VYQ93_000915"
     CDS             complement(11577..11906)
                     /gene="crcB"
                     /locus_tag="VYQ93_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV3335625.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(11900..12274)
                     /gene="crcB"
                     /locus_tag="VYQ93_000916"
     CDS             complement(11900..12274)
                     /gene="crcB"
                     /locus_tag="VYQ93_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV3335626.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(12271..12537)
                     /locus_tag="VYQ93_000917"
     CDS             complement(12271..12537)
                     /locus_tag="VYQ93_000917"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV3335627.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVGSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(12653..13096)
                     /locus_tag="VYQ93_000918"
     CDS             complement(12653..13096)
                     /locus_tag="VYQ93_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV3335628.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            13200..14135
                     /locus_tag="VYQ93_000919"
     CDS             13200..14135
                     /locus_tag="VYQ93_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV3335629.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            14189..14473
                     /locus_tag="VYQ93_000920"
     CDS             14189..14473
                     /locus_tag="VYQ93_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV3335630.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(14530..14748)
                     /locus_tag="VYQ93_000921"
     CDS             complement(14530..14748)
                     /locus_tag="VYQ93_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335631.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(14745..15319)
                     /locus_tag="VYQ93_000922"
                     /pseudo
     CDS             complement(14745..15319)
                     /locus_tag="VYQ93_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4664 a         3427 c         2854 g         4473 t
ORIGIN      
        1 tagtgctata ctatagggaa agagtacatc agatcaagga ggatgctcac atggaagaca
       61 agaaactcat tcaactccta tccaagttaa ataaaagcta ccaaaactgt aaacaggtta
      121 cggcagatga tattcgacta caagagctgc taaacactac tatgcaagag ctcaaaaaac
      181 agaaaagttg aacaacagta tcttaattaa tcttgagaaa ttttaccaac ccaccagtcc
      241 tctgattgga ctgggtagcc taaaactaaa caatcaagca cgcactgctt ggcgaaacta
      301 tgataaaatc cattacgagt atgtcaaaca cgtactaagt ctctatggac ctgtttttgg
      361 attttagagc atagaatttc cagttttctg ttgacaaaat ttccttaaag gtataatata
      421 aagatactaa tactcggagg taagggagac atgaacaact aagtctatca aataaagaac
      481 ctttatttag tagatcttgt ttttgtctct ttttgtgtgc tcttttatgc tctttttctg
      541 gcatgttaat agagtttttt tgacatagac tttgggctct actaggtaaa gtagagcttt
      601 ttgttatgca ctatcgacat tctagaaagg gcaatagtat gataaaaatc aatcacctga
      661 ccatcacaca aaacaaagat ttacgagacc ttgtctctga cctaagcatg accatacaag
      721 acggggaaaa ggtagctatt attggcgaag aaggaaatgg taaatcaacc ttacttaaaa
      781 ctttaatggg ggaagatttg tctgatttca ctatcaaggg agacatccaa tctgactatc
      841 agtcactggc ctacattcct caaaaacttc ccgaggagct gaaaaagaaa actttacacg
      901 actacttctt tttagattct attgatttag actacagtat cctctatcgt ttggcggagg
      961 aattgcattt tgatagcaat cgtttcgcaa gtgaccaaga gattggcaat ctatcagggg
     1021 gagaagcttt gaaaattcag cttatccatg agttagccaa accctttgag attctatttt
     1081 tagatgaacc ttcaaatgac ctagaccttg agacagttga ttggctaaaa ggccagattc
     1141 aaaagaccag gcaaaccgtt attttcattt cccatgatga agactttctt tctgaaacgg
     1201 cagacactat tgttcacttg cgactggtca aacaccgtaa agaagcggaa acgctagtag
     1261 agcatttaga ctatgatagc tatagtgagc agagaaaggc taattttgcc aaacaaagtc
     1321 agcaagctgc taacaaccaa agagcctacg ataaaaccat ggaaaaacat cggagagtta
     1381 agcaaaatgt agaaactgcg cttcgagcta ccaaagatag tactgccggt cgcctattgg
     1441 ctaaaaagat gaaaactgtc ctctcacaag aaaaacgcta cgaaaaggca gctcagtcca
     1501 tgactcaaaa accacttgaa gaggaacaaa tccaactttt cttttcagac atccaaccat
     1561 taccagcttc taaagtctta gtccaactgg aaaaagaaaa tttgtccatt gacgaccgag
     1621 ttttggttca aaaactacaa ctaactgtcc gtggccaaga aaaaatcggt attatcgggc
     1681 caaatggtgt tgggaaatca actctgttag ccaagttaca gagacttctg aatgataaaa
     1741 gagagatttc acttggtttt atgccacaag attaccacaa aaaactgcaa ttggatttat
     1801 ccccaatagc ctatctcagt aaaactgggg aaaaagagga actacagaaa atccaatctc
     1861 acctagctag tctcaatttc agttatccag aaatgcagca tcaaattcgc tccttatctg
     1921 gcggacaaca gggaaaactc ctgcttttgg atttagtcct gcgcaaacca aactttctcc
     1981 tgctggatga acccacacga aacttttctc ccacttctca accccaaatc agaaaactct
     2041 ttgccactta tcctggcggt ctcatcactg tttcgcatga ccgtcgtttc ttaaaagaag
     2101 tctgctcgat catctatcgc atgacagaac acggtttgaa gctagttaat ttagaagatt
     2161 tataaatttg caacatagca gataggtggg gcaaaaacac aattttagga gaaaaagaag
     2221 taaatcttcc catagtaaaa cgcataatat caagttttca cacctgatat tatgcgtttt
     2281 tcagatttta aagacttttt tccagccttc attttacata tgttttaaac gttcaatccg
     2341 ttctgagata ggtgggtggg tataaaagag tttttggaac cccccacctt tcttgggatc
     2401 attgatataa agggtactgc tagcatcatc gacgtggcga ctcataggtt tgctattgtc
     2461 caacttatct agggcattaa tcattccctg gggattgcga gtcagctcga cactagatgc
     2521 atctgccaga aattccctct gacgagaaat agcgagctga accaaggttg cagcgagagg
     2581 tgccagtaca atagctagta gggaaaccac tagcataatg atttcaagac catttccatc
     2641 tcggtcatca tcacttcgtc tgcgacctgc tccaccccac cacatcatac gacctgccat
     2701 actagaaagc atggtgatag cactagcaag ggcaactgca atagtcgaaa tacggatatc
     2761 ataattacga atatgactga cttcatgtcc cataacagct tctagttctt cacgattcat
     2821 gatagctagt agacctgaag tcgcagcaac agccgcattt tgaggattag aacctgtcgc
     2881 aaaggcattt aaggctggat catcaatgat gaaaacacgg ggcataggaa tctgagcgac
     2941 cagagccata tcttccacta catggtagag gtctggtgcc gtttgctcat ccacctcacg
     3001 cgctccattc atggacatga caatctctgt cgattgaaaa atcatagaca aagcgtagat
     3061 aaagccgata atcagtgcga taaccaaacc accaagtcca gatcttataa agagataacc
     3121 aaccgcataa ccaacaagag ctaagagtag gaaaaatacc agcaacaaaa tccaggtttt
     3181 tcgtttattg cttgcaattt gatcaaacaa catcttagtc acctaaaccg ctaaaatcaa
     3241 ctttaggaac cgacttttcc tcttcaggtg tttgaaggaa atctgccgct ttaaatccaa
     3301 acattccagc gataatattg ctcgggaaag tttctaattt tacattgtag ttgctgacaa
     3361 cactgttata gagttgacga gagtaagaaa ttttattttc tgtgtttgtc aactcctctt
     3421 gcaatttaac aaagttagca ctagctttca aatctggata gctttctgca actgcaaaaa
     3481 tacctgaaac ttgacgagtg agggcatcac tggctttcat agcttctgct ggtgaagtcg
     3541 ctgccgccac ttggttacgt agttctgcca ccttttcaag ggtagaacct tcatatttgg
     3601 cataaccttt tacagtctca atcaagtttg gcaagaggtc atttcgacgt ttcaactgaa
     3661 catcaatctg actccaagcc tccttggttt gcatacgatt tttaaccaaa ccgttatagc
     3721 taacaatcac aaaaataaca ataagagcga taactccaag aataatccaa gtcatgatat
     3781 aagtcctttc tacttttaga ttagtaccag tatatcaaat ttttaatgat tgtggtaaaa
     3841 taagatgata ctaaagaagg aaataactat gaaaccagaa acattttaca acttgcttgc
     3901 cgagcagaat cttccacttt cggaccagca aaaagaacaa tttgaacgtt attttgagct
     3961 cttggtcgag tggaatgaga agattaattt gacggcgatt acggacaagg aagaagttta
     4021 tctcaaacat ttttacgatt cgattgcacc cattcttcaa ggtttgattc ccaatgaaac
     4081 tatcaaactt cttgatatcg gggctggggc aggatttcct agtctaccaa tgaaaattct
     4141 ctatccggag ttagatgtga ccattattga ttcactcaat aagcgcatca acttcctaca
     4201 actcttggct caagaactgg atttgaacgg agttcatttc taccacggac gtgccgaaga
     4261 ttttgcccaa gacaagaact tccgtgctca atatgatttt gtaacagctc gtgcggttgc
     4321 ccgtatgcag gtcctatctg aattgactat tccctacctt aaggttggtg gaaaactatt
     4381 ggcactcaag gccagcaatg cgcctgagga gttgttagaa gctaagaatg ccctcaatct
     4441 cctttttagt aaggtcgaag acaatctcag ctacgcccta ccaaatagag atccgcgtta
     4501 catcacagtg gtagaaaaga aaaaagaaac accaaataaa tatccacgta aggctggtat
     4561 gccaaataaa cgcccgcttt aaatttttaa gtaaaaaaat atttacaaaa tcaacctcgc
     4621 tcttttattt ctaggctcgg gaaaaaatga tttacaaaat cagcctcgct cttttatttc
     4681 taggctcggg aaaaaatgat ttacaaaatc atttttttct gctatactat cctaagcaaa
     4741 ggtttttaat gtcatcccgt gaggtgacga agacgcaaaa atatttgaaa ctctttaaag
     4801 tctagatttt agagaagtct tactctgagg gcctattgct gtaaaataat gggctctttt
     4861 ctgatgccca aaagtgaggt ttatatgaaa caagaatcaa ctgttgattt gttattagac
     4921 gttgaccaac gtccttcggc tggaaaagga attctcctta gcttccaaca cgttttcgcc
     4981 atgtttggtg cgaccatctt ggtaccattg attttgggaa tgcctgtatc tgttgccctt
     5041 tttgcttcag gtgttggaac actcatctac atgattgcta ctggttttaa agttccagtt
     5101 tatctaggtt cttcatttgc ctttatcaca gctatgtcac tggctatgaa agaaatgggg
     5161 ggtgatgtat ctgctgccca aacaggggtt atcttgactg gtttggtcta tgtccttgtt
     5221 gctaccagca tccgatttgt aggaacaaaa tggattgata aactcttgcc accaatcatt
     5281 atcggtccta tgatcatcgt tatcggtctt ggacttgcag gttcagctgt taccaatgca
     5341 ggtcttgtag cagacggaaa ttggaaaaat gctctggtag ccgttgttac tttcctaatt
     5401 gctgccttta tcaatacaaa aggaaaaggc ttcctacgaa tcattccatt cctctttgcc
     5461 attatcggtg gttacctttt cgcactaact cttggcttgg ttgactttat accagttctt
     5521 aaagccaact ggttcgaaat tcctggtttc tacttgccat ttagcacagg tggtgccttt
     5581 aaagagtaca atctttactt tggtccagaa gccatcgcta tcttgccaat cgctatcgta
     5641 acaatttctg aacatatcgg agaccatact gttttgggtc aaatctgtgg tcgtcaattc
     5701 ttaaaagaac caggtcttca ccgtactctt cttggtgacg gtatcgcaac ttctgtttct
     5761 gccttccttg gtggaccagc caatacaact tacggagaaa atacaggggt tatcggtatg
     5821 actcgtatcg cttctgtctc agttatccgt aacgctgcct tcatcgcgat tgccctcagc
     5881 ttccttggta aattcactgc cttgatttca actattccaa acgctgtact tggtggtatg
     5941 tcaatccttc tctatggggt tatcgccagc aatggtttga aagtcttgat taaagaacgt
     6001 gttgatttcg ctcaaatgcg aaacctcatc atcgcaagtg ctatgttggt tcttggactt
     6061 ggaggagcta tccttaaact tggtccagtt acactttcag gtactgccct ttcagccatg
     6121 acaggaatca tcttgaactt gatcttgcca tacgaaaata aagactaaga gtctaaatac
     6181 acctaatcca ctcagacagc tgagtggatt tttcgtatac cataataaaa gtgtcttaac
     6241 aaaattatta aaatcaaaaa acgtataata tcagatattc taaaaccttg atactgtacg
     6301 ttttatcata gaaattttta ctttattttc tcatcaaatg agatttgcat caatctcttg
     6361 tcttacttgc gtttcttctt cgctttcttc attttgttag ccatacgttt catggactgt
     6421 ttcatggcaa attcaccaat tttacctttc aaaccgccac caaacatctg gctcatatct
     6481 ggcattcctg ctcctccgag agctgataag tcaggcatac cgccttgtcc catcattcct
     6541 tcaagggcag acatatccat tcctcccata tttggcatat ttttaggaag gttatttgga
     6601 ttaatcccca tttgcttcat cattttattc atatccccag acataacacc ctgcatgagc
     6661 tgtttagcct ggttaaagtc cttgatgaat ttattgactt cgacgaatgt atttccagaa
     6721 ccagcagcaa tacgacggcg acggcttgga tttaacaaat ctgggttttc acgttcttca
     6781 ggtgtcatcg aagacacaat cgcacgttta cgagcaatct ggcgttcatc caccttcatg
     6841 ttttgaaggg ctggattgtt ggccatacct ggaatcatct tgagcaagtc ttccatcggc
     6901 cccatatttt gcacctgatc taattgatcg atgaaatcat taaaatcaaa ggtgttttcg
     6961 cgcatcttct cagccatttc aagggctttt tgttcatcgt attcctgaga agctttctca
     7021 atcaaagtga gcatatcccc cataccaagg atacggctag acatgcggtc tgggtggaag
     7081 gtttcaatgt ccgtaatctt ttcacctgta ccagtgaact tgattggttt tccagtaatg
     7141 tgacgaacag acagagcagc accaccacga gtatcgccat caatcttggt aaggatgacc
     7201 ccagtcactt ccaactgagc attaaactca cgcgcaacat tggctgcttc ctgaccaatc
     7261 atagcatcaa cgacaagcaa gatttcattt ggttgagcca atgctttcac atcacgaagc
     7321 tcattcatga ggagctcatc aatctgcaaa cgacccgcag tatcaatcaa gacatagtcg
     7381 ttatgattag tttgggcttg ctccaaacct tgacgtacaa tctcaacagc tggtacttct
     7441 gttccaagtg caaagacagg cacatcaatc tgttgtccca aggtcttaag ctggtcaatg
     7501 gcagctggac gataaatatc cgccgcaatc atcaaaggac gagcattttc ttctttcttg
     7561 agtttgttgg ccaatttacc agcaaaggtt gttttaccag ccccttgtaa accaaccatc
     7621 atgatgatgg ttggaatctt aggtgacttg ataatttctg ccgtatcaga acctaaaacg
     7681 gctgtcagtt cctcatcaac gattttaata atctgttgcg caggattaag tgtatcaatg
     7741 acctcatgcc cgactgcacg ctcacgaact ttcttgataa agtcctttac aacaggcaag
     7801 gcaacgtcgg cctcgagcaa ggccaagcga atttctttgg ttgcctcttg gacatcagat
     7861 tcagagattt ttcctttttt acgtagattt ttaaagacgt tctgcaaacg ttctgttaaa
     7921 ctttcaaatg ccatttttct tcctcttatt ctctattatc aatgcttgtt aaaatttcta
     7981 tctgctcctg cagaaagtca tccttgggat agcgctccaa aatctgatca aaaatctgac
     8041 tgcggacaat atagtccgag tacatgtgca atttcatctc ataatcttcc agaatctttt
     8101 ctgttcgctt gatattgtca tagacagcct gacgactgac accgaactcc tcggcaattt
     8161 cagcaaggct gtaatcatca gcgtagtaga gctcgatata attcatttgc ttatctgtca
     8221 aaagcgccgc ataaaattca aagagcgcat tcatacgatt ggttttttcg atttccataa
     8281 cttttattat accaaaaatt agcctaatct accacactag gaagccgatc caagaagata
     8341 gatagctaaa tttgaaaaag acatgagcct agccccaagt aatttccaat tgatagctgg
     8401 caaagggatg tccctcttga ttttgtagtt gataatctag ttcaatcttt tgcctatcaa
     8461 cttgataatg gctcgtttgg atggtaaact cctgcatgcc cataggtgta agaatatagg
     8521 ctaaactatc gctatccttt agaaagcgca taatggtctt gggattagaa aatcggctca
     8581 tcacaagttc ttgaccatga aatttaatca ctactttttc cttttcctca ttatagaaaa
     8641 gcaggtagct ataatctctt ttttcatgca cttccacatc ataaagctgg tcaatcactt
     8701 ccaactgctc atcaaactga atcgtatttc gcatccgaat cttcacatca ggccctcttt
     8761 cttgtctctt gtcctactat tttaccaaaa agagcaggat tttgctataa tggtcatatg
     8821 aacgaaaaag tattccgtga cccagttcac aactacatcc atgtcaataa tcaaatcatc
     8881 tatgacttga ttaatacaaa agaatttcag cgtttgcgcc ggatcaaaca actgggaact
     8941 tccagttata ccttccacgg tggagaacac agtcgcttct ctcactgtct aggagtctat
     9001 gaaattgcac gacgcatcac agagattttc gaagaaaaat atcctgagga atggaatcct
     9061 gccgagtctc tcttgaccat gatcgctgct ctcctacatg accttgggca tggcgcctac
     9121 tcccatactt ttgaacatct ctttgataca gaccatgaag ctattactca ggagattatt
     9181 caaagtcctg agacagagat tcaccaagtc ttgctacaag tagcgcccga ttttccagag
     9241 aaagttgcca gtgtcataga ccatacctat cctaacaagc aggtcgtaca actcatttct
     9301 agtcagattg acgcagatcg catggactat ctcttgcgtg actcctattt tacaggagca
     9361 tcctatgggg aatttgacct gactcgaatc ctccgagtca ttcgtcctat cgaaaatggt
     9421 atcgcctttc agcgcaatgg catgcacgcc atcgaagact atgtcctcag tcgctaccag
     9481 atgtatatgc aggtctattt ccacccagca acacgcgcta tggaagttct cctacagaat
     9541 cttctcaagc gcgctaagga actctaccct aaggacaaag acttctttgc acgaacttcc
     9601 ccacacctcc tgcctttctt tgaaaaaaat gtgaccttgt ctgactatct ggctctagat
     9661 gatggtgtga tgaataccta cttccagctc tggatgacca gtcctgacaa aattctcgca
     9721 gatttatcgc aacgctttgt caaccgcaag gtctttaaat ccattacctt ttcacaagaa
     9781 gatcaagacc aactcgctag catgagaaaa ttggttgagg acattggctt tgatcccgac
     9841 tactatactg ccattcataa gaactttgac ctcccttatg atatctatcg tcccgaatct
     9901 gaaaatccac ggacacagat tgagatttta caaaagaatg gagaactggc cgaactctct
     9961 agcctgtctc ctatcgtcca atcccttgct ggtagtcgtc acggagataa tcgtttctat
    10021 tttccgaaag aaatgttaga tcaaaacagt atctttgcta gcattaccca gcaattttta
    10081 tacttgattg agaacgatca ttttacccca aataaaaact agaagaggaa atttatgagt
    10141 attaaactaa ttgccgttga tatcgacgga acccttgtca acagccaaaa ggaaatcact
    10201 cctgaagttt tttctgccat ccaagatgcc aaagaagctg gtgtcaaagt cgtgattgca
    10261 actggccgcc ctatcgcagg cgttgccaaa cttctagacg acttgcagtt gagagacgag
    10321 ggggactatg tggtaacctt caacggtgcc cttgtccaag aaactgctac aggacatgag
    10381 attatcagcg aatccttgac ttatgaggat tatctagata tggaattcct cagtcgcaag
    10441 ctcggtgtcc acatgcatgc cattaccaag gacggtatct atactgcaaa tcgcaatatc
    10501 ggaaaataca ctgtacacga atcaaccctc gtcagcatgc ctatcttcta ccgtacccct
    10561 gaagaaatgg ctggcaaaga aattgttaaa tgtatgttta tcgatgaacc agaaattctc
    10621 gatgctgcga ttgaaaaaat tccagcagaa ttttacgagc gctactccat caacaaatct
    10681 gctcctttct acctcgaact ccttaaaaag aatgtagaca agggttcagc cattactcac
    10741 ttggctgaaa aactcggatt gaccaaagat gaaaccatgg caatcggtga cgaagaaaat
    10801 gaccgtgcca tgctggaagt cgttggaaac cccgttgtca tggaaaatgg aaatccagaa
    10861 atcaaaaaaa tcgccaaata catcactaaa acaaatgacg aatccggcgt tgcccacgcc
    10921 atccgaacat gggtactgta aaaggaaaga atagactaaa accgctcagc tgagcggttt
    10981 ttgatgtcta attacatgcc ccctgcagga atcgaacctg caactactcc ttaggaggga
    11041 gttgttatat ccattgaact aagggagcta gataaaaact ctgctgaaaa agcagagttt
    11101 tttagtcgaa ttaacgacgg atttctttga tacgagctgc cttaccttga agagcacgca
    11161 agtagtacaa tttcgcacga cgtactttac cgtaacgtac aacttcgatt ttttcaacac
    11221 gtggagtgtg gattgggaag atacgctcaa cacctacacc gttagagatt ttacgaactg
    11281 tgtagttttc tgagattcca gcacctttac gtgcgataac aacaccttca aaaatctgga
    11341 tacgttcacg gttaccttcg acaactttcg catgtacacg aacagtgtca ccaggacgga
    11401 atgatgggat atctgtacga agttgacctt cagtcaagct ttggattaat ggattcattt
    11461 tattctccta tctttgtcaa tcttgaggaa tcttcctcag cggataaact gtatttttgt
    11521 gcgtccatta cacacaagat atagtttacc aaatttctaa aaaaaagtaa agaaatttat
    11581 agcaaaattc ctaaaaaaat cgcaaccaaa ccacctatgt aagtcaaagt caggtaagaa
    11641 taaaagacct tcttatcact tagcagtctt tgaagttcgt cattcaaggt cgaaaaagtt
    11701 gttaaaccgc cacaaaatcc tgttgctaga atagcatata cttccttaga ctctacatga
    11761 ttgtaaaata ctccaattaa aaaacaacct aaaagattag ctatgagcgt tccgagtggc
    11821 aatttagaag cttgattata gcgagaaaag aaataccgta cgagtgctcc caagccacag
    11881 gcgattgcaa gatagacgat taccatttct tcctccctag aaaataagct aagagtaggc
    11941 ctccaccgat gctcaaaagc aaatacagga ctaaactaaa ataacgccct gtatcaagca
    12001 gcttcacagt atcaagcatt aggctggaaa aagttgttaa acctccgcaa aaacccgtcc
    12061 ccagtgctaa aatcaagccc ttactagtcc ccttatagac cagatagccc ttgacaagaa
    12121 aaatcaagca gaaaattccc agatagttga caaagagagt gccccaagga aagtctggac
    12181 tggctggtaa ccaggtggaa actagatatc ggacaagtcc gcccaacata gcagctagaa
    12241 aaattcctag cggataaaat tgttcttttt tcatttgata ttttgatcct gataatcacg
    12301 cgaacgtttg agtatgtccg aaaaagtcgc tacaatagtc tcctgatagc gcttgtcctc
    12361 tacacgactc ccaacctttt caaaaataac ttcttctctc ttagtatcta aaatcggttt
    12421 acctgaagct ttcttataag caaccacccc ctcaactaaa tgcattcgtt cttctaacag
    12481 tttgacaatt tggtcgtcga tttgatcgat ttcttgacga ataatatcta aatccataca
    12541 gtctcctcct ttatttgaat tattgtatca aaaagccatc aattaggcta gtaaaatccc
    12601 aactctcgat aagaaaaatg cactgattga ttacatcagc gcacttttat gcttatccta
    12661 ctttagcaac caattcttct gcgaattgtt ccaagcgttc aatatcttct tcctcggcag
    12721 aaagatcaac tttgacacac tcagaacctt tttctgctcc tgttgaaaca aagacacggt
    12781 caaagtcatc aacagcctta cagaattcat cgtagaaggt atctcctgag ccgaccactc
    12841 cgtagatttt gccattcaag ttgagatctg ctagatcttc gtagaagtcc atcatctcat
    12901 ctggcaattc accatcacca taagtatagg tcgcaacgat agcgatgtct gcttccaaga
    12961 agtctgaagc gtcaacagtt gtacattcat caacatcaac atccaagccc aagtcacgta
    13021 atttgtctgc tacaatatct gcaatttctt cggtattacc ggtcatactg gcaaatacaa
    13081 tttttgctaa tgccatatcg tcctcctcaa tttatctttc tccattatat cacatctttt
    13141 tcattttaaa aataaaaatt cttgtgctac aatgaaatga gaaagaattg aggttattta
    13201 tggagatttg ccaacaaatt ttagaaaaaa ttaaagaata cgacaccatt atcattcatc
    13261 gtcatatgaa accagaccct gatgccttgg gaagtcaggt gggattgaaa gccttgctgg
    13321 aacatcattt cccagaaaaa accatcaaag ccgtcggttt tgatgaacca actcttactt
    13381 ggatggctga gatggatctt gttgaagata gagcctacca aggcgcactt gtcatcgtct
    13441 gtgatacagc taatactgct cgtatcgatg ataagcgcta tagtcaaggt gattttctca
    13501 ttaagattga ccaccatcca aatgatgatg tatacggtga cctgtcttgg gtcgatacta
    13561 gttcaagtag cgctagcgag atgattaccc tatttgccca aacaacccaa ctagccttgg
    13621 cagatcgcga tgctgagttg ctctttgcag gaattgtcgg tgatacaggt cgcttcctct
    13681 acccttctac cactgcacgg actcttcgcc tggctgctta tttgagagaa cataactttg
    13741 actttgcggc tctcactcgc aaaatggaca ctatgagcta caaaattgct aaactgcaag
    13801 gctacatcta cgaccatctg gaagtggatg aaaatggtgc tgctcgcgtt atcctgagtc
    13861 aagaagtctt gaaacaatac aatgtaaccg atgctgaaac tgcagccatt gtcggtgcac
    13921 ctggacgcat tgacagagtg agtctctggg gaatttttgt cgaacaggct gatggccact
    13981 accgagttcg cttacgcagt aaaatccatc ctatcaatga aattgccaag gagcatgatg
    14041 gtggaggcca ccctctagca agtggtgcta attcctatag cctagaagaa aacgaaatca
    14101 tctaccaaaa gttaaaaaac ttgcttaaaa actgataaaa tacttgccaa acttttcaga
    14161 atctgataga ctagtatggt aacaatctat ggctcgcaaa gagaccatgg cagaaaggaa
    14221 atattgcaaa atgaaaaaag atatccatcc agaatatcgc ccagttgtct tcatggacac
    14281 aactactggt taccaattcc ttagcggttc aacaaaacgc tctaacgaaa cagttgagtt
    14341 cgaaggcgaa acttacccat tgatccgtgt ggaaatttca tcagactcac acccattcta
    14401 cactggacgt caaaagttca ctcaagcaga tggacgcgtg gatcgtttca acaaaaaata
    14461 cggtctcaaa taatgataaa agaacagttt cgactgttct tttttgtttc ttgaaatcaa
    14521 ctgctgtttt catgttccag actcatctgt aggttcggtt tccatgctac taggcaggaa
    14581 ggaaatagct gtttcaacac gtccataatg agctgtacta ttgtcacgaa ccacactttc
    14641 attgatggtc caagtggaat tcattttctt aaaagcttct cggacttttt ccaaatcttt
    14701 ggaggcaatg gcctgctcta aggtttcaaa acgaggactt atactcatct gctttcacaa
    14761 agcattctag tccatctccg attaacgatg gactttatca cctccttctc cagtccttgt
    14821 ataacatctt ggagttgatt catgacatct tccaaagttc gaaaggcttt attcttaaat
    14881 ccacgtttac gaatctcttt ccacacttgt tcaatgggtt catctctggt gtgtatggag
    14941 gaataaaggt aaaatcaata ttagtcggaa tatttaaggt acttgattta tgccatatag
    15001 cattgtccat aacgagtaaa aggataagct tgtgaaagct cttctaaaaa ggcgttcatc
    15061 cacactcctt tttataagcc tgaaataagg catcaattgt aacaaattct cctgcctctg
    15121 cagccttcaa atggcgagca agaaagactt tcttttcctc aactgtcata tatgcatgat
    15181 tacgaccacc acgtgtttct tgaaggagag agtcgagtcc gaactcctca tagtttttta
    15241 catttcgcca aatcgttgtt tgattacagt ctaaaagttc tataatctct ttataagatt
    15301 tgcccatcag acgaaatata gtagattgaa actagaatag tacacctcta cttctaaaac
    15361 attgttagaa atcgatttga ctgtcctgat cgatttgctc tgttcttgtt tcgtttta
//
LOCUS       DAVMGZ010000035        15022 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000035 DAVMGZ010000000
VERSION     DAVMGZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15022
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.036"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(148..1344)
                     /gene="tuf"
                     /locus_tag="VYQ93_000923"
     CDS             complement(148..1344)
                     /gene="tuf"
                     /locus_tag="VYQ93_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV3335632.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2255..3124)
                     /locus_tag="VYQ93_000924"
     CDS             complement(2255..3124)
                     /locus_tag="VYQ93_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV3335633.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3347..3868)
                     /locus_tag="VYQ93_000925"
     CDS             complement(3347..3868)
                     /locus_tag="VYQ93_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV3335634.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDEK"
     gene            complement(3826..4905)
                     /locus_tag="VYQ93_000926"
     CDS             complement(3826..4905)
                     /locus_tag="VYQ93_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV3335635.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSYRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEELAE
                     LISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNVTDKKLSSMT
                     TTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANYYKEGRQEKL
                     AESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPANAYQKTVQQ
                     LTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5016..5270)
                     /locus_tag="VYQ93_000927"
     CDS             complement(5016..5270)
                     /locus_tag="VYQ93_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335636.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(5628..6161)
                     /locus_tag="VYQ93_000928"
     CDS             complement(5628..6161)
                     /locus_tag="VYQ93_000928"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV3335637.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPVLYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVMDLL
                     REVPTIFECLECFFKTR"
     gene            complement(6148..6363)
                     /locus_tag="VYQ93_000929"
     CDS             complement(6148..6363)
                     /locus_tag="VYQ93_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3335638.1"
                     /translation="MGKERREQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(6330..6473)
                     /locus_tag="VYQ93_000930"
     CDS             complement(6330..6473)
                     /locus_tag="VYQ93_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEV3335639.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            6707..8425
                     /locus_tag="VYQ93_000931"
     CDS             6707..8425
                     /locus_tag="VYQ93_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV3335640.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8536..8883
                     /locus_tag="VYQ93_000932"
     CDS             8536..8883
                     /locus_tag="VYQ93_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV3335641.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9162..9998)
                     /locus_tag="VYQ93_000933"
     CDS             complement(9162..9998)
                     /locus_tag="VYQ93_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3335642.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10011..10640)
                     /locus_tag="VYQ93_000934"
     CDS             complement(10011..10640)
                     /locus_tag="VYQ93_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3335643.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(10650..11291)
                     /locus_tag="VYQ93_000935"
     CDS             complement(10650..11291)
                     /locus_tag="VYQ93_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3335644.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11437..12666)
                     /locus_tag="VYQ93_000936"
     CDS             complement(11437..12666)
                     /locus_tag="VYQ93_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV3335645.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELYDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILELQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEYYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(12656..13822)
                     /locus_tag="VYQ93_000937"
     CDS             complement(12656..13822)
                     /locus_tag="VYQ93_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV3335646.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            13966..14979
                     /locus_tag="VYQ93_000938"
     CDS             13966..14979
                     /locus_tag="VYQ93_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV3335647.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
BASE COUNT         4524 a         3210 c         2687 g         4601 t
ORIGIN      
        1 aatatgtact attctagttt caatatatta tagtttgctc tttgattttc attgagtatt
       61 agttcaatat ctaaaaaaag aaccttgcca agcaagattc ttttagtgtc tgacttaaat
      121 aattgttctt ctgggaacta aatcgaatta agcttcgatt tctgtaacca tacctgaacc
      181 aacagtacgt ccaccctcac ggatagagaa tgtagtacct tgttctacgg cgattgggtg
      241 aatcaactca acgtcgattg tcacgttatc accaggcatt accatttcag tacctgctgg
      301 aagttcgatt gaacctgtaa cgtcagtagt acggaagtag aattgtggac ggtagttgtt
      361 gaagaatgga gtgtgacgtc caccttcttc tttagtaagg atgtagactt cacctttgaa
      421 tttagtgtgt gggttgattg aacctggttt agcgataact tgtccacgtt cgatttcatc
      481 acgttgaaca ccacgaagaa ggacacctac gttatctcca gcaagacctt cgtcaagttg
      541 tttacggaac atttcaacac cagtaacaac tgctttttga gtttcttctt tgataccaac
      601 gatttcgatt tcgtcgttga ctttaacgat accacggtcg atacgtcctg aagcaactgt
      661 accacgtcca gtgattgaga atacgtcctc gactggaaga agcaatggtt tgtcagtgtc
      721 acgttctggt tctgggatat actcatcaac tgtgttcatc aattccataa cgatgtcttc
      781 gtatttagag tcaccttcaa gagctttaag tgctgaacct tggataactg gaagatcgtc
      841 acctgggaag tcgtattctg acaataggtc acggatttcc atttcaacca attcaagcaa
      901 ttcttcgtcg tcaaccaagt caactttgtt catgaagacg ataaggtgtt taacaccaac
      961 ctgacgtgaa agaaggatgt gctcacgagt ttgtggcatt ggtccgtcag ttgaagctac
     1021 tacaaggata gctccgtcca tttgagcagc accagtgatc atgtttttaa cgtagtccgc
     1081 gtgtcctgga gcgtcgatgt gagcgtagtg acgtttttca gtttcgtact caacgtgcgc
     1141 agtgttgata gtgataccgc gttcgcgttc ttctggagca gcatcgatag acgcatagtc
     1201 tttaggttgg ttaactgatg aaggcaagcg acgtgccaaa acagttgtga tagctgcagt
     1261 tagggtagtt ttaccgtggt caacgtgtcc gatagtacca atgttaacgt gtggtttact
     1321 acgatcgtat ttttcttttg ccatttgagt aaaagcctcc aataaaatat attttataga
     1381 tagacagtag gcaatacagt ctaactttcc ttactatttt atcaaatttc agctaaattg
     1441 caagtgtttt acaacgtttt tgtgaattat tcttggtaaa aatttaacta actaatagaa
     1501 agtactaaat agacttatcc ttcaaggagt tttcagcatt atccatccac tcacttgctg
     1561 aaaatgtaaa cattctctgg tttatatagt aaaatgaaac aagaataggg caaatcgatc
     1621 aggacaatca aatcgatttc taacaatgtt ttagaagttg aggtgtacta ttctagtttc
     1681 aatctactat acaacctcaa aactgtgttt tgaacaacct gcggctagct tcttagtttg
     1741 ccctttgatt ttcattgagt ataaaattgc agataaaacg ataacaatca taggactcag
     1801 cagagtcaat gtaggtcgaa gaatagcccc cctaatcgct tattttatag caaaatgaaa
     1861 tagaaactga acattttgtt gaggaaggta aatcaattcc tcacaatgtt tgagaaagaa
     1921 gggaggacta ttccaacttc aacccactat aaagcagact tacaagatag ggaacccatc
     1981 tccgataata tatagtagaa tgaaaactgc acaatttgat tggagaattc aaaccaattt
     2041 ctaacaatgt tttagaaaca agggtctatt atttcaactg caacctacta tagcccattg
     2101 aagctggaat agcccactga gacttctaaa ccactgctgg agaggagttt gtttagagtt
     2161 tctttcattt ccctaaaaaa tcaaaaagag agaaccagtc tgatcctctc ttacttatag
     2221 atggtgctca ctcaaatgtt aaaggagcta tttcttagag ataaaggaat ttgaatacag
     2281 ctactgccgc aattgctgct gcgataggtg ctactactgg tacccaagaa taccaccatt
     2341 ttgaatcgcc tttatgctca ccaagaactg attttggaag gaaagcatga aggagacgtg
     2401 gtcccaagtc acgggctggg ttcaaggcag gtcctgtagg tcctccaagt gatgttacca
     2461 aagccataac gaggaatcca agtgccaagt gagccacaga aagtcctgaa gcagtgtgtg
     2521 gagccacctg tgctttaata gccaagtcag aaaaatcaac tgtttgtcct gcttctattg
     2581 ccttttgttt catgaattga agcacttcag caccaaagaa gtttttagtc aaaccaagag
     2641 ctgcaaagaa caaaacaaat gaaccaacaa actcattaat caaaccattg acagttgctg
     2701 catagcgact ttcttttgta ccatggtcaa tacttgaaat agttgagaaa gttcccaaga
     2761 tgttatttgg tttttcagtt ttcaagtaga atggacggta tgttgccaca actaaggctt
     2821 ggccaaagat agcccccaag acttgcgcga taatgtaagg taccacttgt gcccaaggga
     2881 aaagaccact aactgcaagc cctagagtga aagcagggtt gatgtgattc ccagatacgt
     2941 taccaaacat caaggctggg atcataaccc ccataccata accaacagcg atgacgatcc
     3001 agccactttg gtgacctttc gtacctttaa gttcaacgtt ggcaactgca ccattcccaa
     3061 gaatgatcaa aatggcagtt cccaaaaatt cagtggcata cttcagtgcc catgtgaaat
     3121 ccatttgcta gattctcctt aaaatttttt agatattcct tattttatca atttttaccc
     3181 tatttagcaa ctgatttctg gaaatatttt taaaaaaata ggattcatag tttcccagtt
     3241 caacgtactt gtaagcgttt ttattcttca cgaaagacct cattctcaaa accaggaaaa
     3301 agcccacatc tgtgatcttt tccttgttcc tagattctaa aatctattat ttttcatctt
     3361 cttttttctt gaagaatcct aatcctagac cagctaacaa ggtcatgata ccagcactag
     3421 ccaaggtagc attagcttct gtacctgtgt ttggcaattc ctctctctta cctgtctcat
     3481 aagtcggaac ttctgggtct ggattcactg gagtttcagt ttttggagta cctggttctg
     3541 gagttggttt atctggtgtt gataaacggt cataccttac cgttatttct ttatcactag
     3601 agtctgacgt aacttcttgt gattcaactg ttggaatatc tggatctttg tacttgtcaa
     3661 tcttaccaga tataacctcg tcccagtttc ctgttgtcca ttcaccgtag gttacaactc
     3721 ccgtgacctt gttctcagtt tttgtacggc ttaaggttac aggttgaaca acatcttctt
     3781 ttacattttg gttcgtaact ttatcaacgt aatgaatgat acgcgttata gtcttcgtct
     3841 cagtagaggt tgctgttttg ggaaccactg tttcctcaac attctcacgg tagtaatagt
     3901 caactgttgc accgtcttct ggtacgcatt tgcaggagtt gctaccaagg tgtatgttgt
     3961 tttccttgtg ataactcggt cttctttgtc ctcagttgtt gttttccctt caatagtttt
     4021 tgattctgtg gtatactcag aacctatcgc taaatcagct tttataacag actctgccaa
     4081 cttctcttgc ctaccttctt tatagtaatt ggctgttact gtagcagtgg ttggcgcttc
     4141 gctttctcta taaactaagg tcactgttct accttcgctt acaatattcc cagttaaact
     4201 tgcagaattt gtatctgctt ctttaaaagt ataatatttt ccgtcagtag tagtcatgct
     4261 actgagtttt ttatctgtga cattatagct ggtaccaatc agttgttttt tattggtaat
     4321 gtaggttccg tcactttctt tttctccaat tccagtatca tttttcaatg atagcaaacg
     4381 cccttgttca tcaacatagc gaactttcac attttctgag attagttctg ccaattcttc
     4441 ggttattttc cctttctctt cttcgggaat atttagtttt ggaatgattt tttcaacaac
     4501 ggttcgtgat ggttccacag tatcttggat gactgaaaag tcagctagaa ttgggagatt
     4561 atactgaaca cggtaacttt gagtgtttac tcctactctt tcattattct ctgaaaatac
     4621 ttgtacggta taagaaacaa catcttttcc taatagaaca tccccagtag agaaatagcc
     4681 gccttttcct agtttgctat ctccagagcc cacttctttc ctaatcttat cagatagttt
     4741 tttaccagtc agtacattcg ttcgcacaat ccctttgtct acccctacaa agtgggagaa
     4801 ctttttgaac tcttcagaac cagatctagc ccaaccatta ttaagggcat ttgcttttgt
     4861 atttgtattc tctctcaaag gtttggcgat tagaattata ttcatcggca cttagagttg
     4921 ctgctatatc tgactcttga ataccaactt ccttactacc atttctagcg gcagtatatg
     4981 tgtatttatc tgtttctact tctagctgat tatccttaac atgataggta attttctcct
     5041 ggtataagag tcttcagtag tttatatact ctgtcatcat ctttagcttt agaagcagct
     5101 gagtttagcc aagttggggt attaggagta tctttatcaa cctgtttcaa gacattattc
     5161 aagtcatttg ctagcctatt ggttatcctc actgtgaaag ttttcttcca gtctgaacca
     5221 cttttattag acttagatat gctcatgaaa tcctggtcaa cattcaacac aatatcgcct
     5281 tgttttttag tatcttccgt tgcttctgct tcaaattgat catggagttt ttgttgctct
     5341 ggtgtatagt ttttttattt acacgattta atgtactatt ttttatttta gtattaagaa
     5401 ctccattaat acgattttta aactctgctt caaaaaatcc cttaatttgt tggtgcgcaa
     5461 gttcaacatt caaacctttc ttatacaagt cttcattctg atacaagtct tcattctgaa
     5521 cgatttcggc tacacggtga atggtttgat cgtataccgt cttctcttaa ccagcagtat
     5581 ccttagtttt aatgactact cgataggttc ctttagaacc gttatagtca tctagtttta
     5641 aaaaagcatt ccaaacattc aaaaatagtt ggaacttccc ttaataaatc catcactttc
     5701 tttttcaaga tagcccaagc ttgctcaata ggattcaaat ccggtgaata aggtggtaga
     5761 ggtaagaaaa agtgtttatc ttggatgcag agttcatcca aaatgttctt gggatgaaaa
     5821 ctagcattgc ccatgacaat aacatgaggt gtcttcaaag caggtaggag ttgcgttttg
     5881 aaccactcca caaagaaatc gcttgtcatg cttttcttgt aaatcatggg agctataaac
     5941 tctccgtcta cttgtcctgc aacaattgaa gttcgctcaa aacgacgtcc gctaatcttt
     6001 tcatagactt tctcccctct aggagcccct gcataaggac gatagaggta gcggtcgatt
     6061 cctgtttcat caatatatag gactggtaaa tcttttaggt tatccaaaat atcaagaaac
     6121 tcagcgactt tctctggctc ttgttcctta aagctcgtca tcttttttta aagtgacctt
     6181 aatctgcttt aaagctgccc atactgaagg aacagcacaa tcaaaatgtg ccgcaatttc
     6241 ccgtaaaaaa gcatctggat gagcctctac aaaggctttc aattcctcta aaggaatctt
     6301 tcggtttttg acgactcgct ttttcctctc tatgtgtcct tgttcacgtc tttcttttcc
     6361 cacgtgaaga gagttctgac gccaacacca aaaaactttg ctgcctcgac atggctgtgc
     6421 ccctctttga tgtaatctaa tgctcgctgt ttaaaatcag tactatatgc catagcttta
     6481 ttataatatg cttgtttaaa actaagcaac tatatcaatg ttttgttatg agaagactta
     6541 ctttttttac tgaaattgaa tttttcctag cctcaacatc atataacccc aaaattcaat
     6601 ctgacctggt ttgaagagat gttcgaagca gataaggttt tactaaaact aaaaaaaatg
     6661 atataattaa actgattaaa tgttttaaaa ataaaggaga atttgaatgt cttaccaaga
     6721 aaattaccag aaatgggttg attttgtgga gcttcctgac taccttcgtc aagatttgga
     6781 aaatatggac gaaaaaacta aggaagatgc cttctataca aatcttgaat ttggtactgc
     6841 aggtatgcgt ggcttggttg gtgctggtac aaaccgtatc aacatctacg ttgttcgcca
     6901 agctactgaa ggattggctc gtttgattga gtcaaaaggt ggaaacgaga aagaacgcgg
     6961 tgtagcaatt gcctacgata gccgtcactt ctcacctgag tttgcctttg aatctgcggc
     7021 agttcttgct aaacacggca tcaaatctta cgtatttgaa agccttcgtc cgactccaga
     7081 actatcattt gcagttcgtc atctcaactg tttcgcaggt atcatggtca cagccagcca
     7141 caaccctgct ccatttaacg gttacaaggt ttacggtgaa gacggtggac aaatgcctcc
     7201 acacgatgcg gacgctttga ctacttatat ccgtgcaatc gaaaacccat ttgcagttga
     7261 agttgctgat gtggaaactg aaaaagcttc tggcttgatt gaagttatcg gcgaagctgt
     7321 tgacatagaa taccttaaag aggttaagga cataaacatc aacccagcct tgattgaaga
     7381 atttggtaaa gacatgaaga ttgtctacac accacttcat ggtactggtg aaatgttggc
     7441 tcgtcgtgct cttgcccaag caggatttga ctctgttcaa gttgttgaag cgcaagcaac
     7501 tgctgaccca gacttttcaa ctgtaacatc tccaaaccca gaaagccaag cagcctttgc
     7561 ccttgctgaa gaacttggtc gtcaagttgg tgcagatgtt cttgtcgcaa ctgacccaga
     7621 cgctgaccgt gttggtgttg aagttcttca aaaagatggt agctacctca acctttcagg
     7681 taaccaaatc ggtgctatca tggctaaata catcttggaa gctcacaaaa acgctggaac
     7741 tcttcctgaa aatgccgctc tctgcaaatc tatcgtttca actgacttgg taacgaagat
     7801 tgctgaaagc tacggcgcaa ctatgttcaa cgtcttgaca ggtttcaaat ttatcgctga
     7861 aaaaatccaa gaattcgaag aaaaacacaa ccacacttac atgatgggat ttgaagaaag
     7921 cttcggttac ttgattaaac catttgtacg tgataaagat gccatccaag ccgttcttgt
     7981 agttgctgaa cttgctgcct actaccgttc tcgtggtttg acacttgctg acggtatcga
     8041 agaaatctat aaagaatatg gctactacgc agaaaaaaca atctctgtta ctctttcagg
     8101 tgtcgatggt gctgaacaaa tcaaagcgat tatggctaaa ttccgcaaca atgctccaac
     8161 agaatggaac gcaacagcta tcactgtcgt agaagacttc aaggcacaaa ctgctactgt
     8221 tgctgacggt actgttacaa acttgacaac tcctccaagt gatgtgttga aatacacact
     8281 tgctgacggt tcatggattg ccgttcgccc ttcaggtaca gaaccaaaaa tcaagttcta
     8341 cattgcagtt gtaggggaaa ccaacgaaga atcacaagct aagattgcta acatcgaagc
     8401 agaaatcaat gcatttgtaa aataaagacc tataaaagat gagaccaacc aacctcatct
     8461 tttttcgtat caaatctaag caattttaga aactttatgg catactagac ttatcaatga
     8521 aagcgaggta atctcatgga acaattttta gataacatca aagaccttga agtcactaca
     8581 gttgtgcgtg cgcaagaagc tcttgataaa aaagaaactg caaccttctt tatcggtcgc
     8641 aaaacttgcc cttactgccg taaatttgca ggtacattgt caggtgtcgt agctgaaacc
     8701 aaagctcaca tttacttcat caatagtgaa gaaccaagcc aactcaatga tttgcaagca
     8761 ttccgctcac gctatggaat cccaactgta ccaggttttg ttcatattac agatggacaa
     8821 atcaacgtcc gttgcgactc ttcaatgtca gcacaagaaa tcaaagattt cgcaggattg
     8881 taaaaaatca gctaacaagt cttaaaatca caaaaacgca taatgtaagg tgcaaaaacc
     8941 ttgatattat gcgttttatt gttcctaaaa cagttacatc agtatttcat tacagaacat
     9001 tttaattttg gaagtcaaat caatttttaa caatgtttta gaaatcacag tgggctattc
     9061 catattcaat tatccatatt caatcgctat tatcgttttc ctagattaca aaaaaaacta
     9121 cgttttgcta atcaaaacgc agtttcagcc actattttat cttactgtcc ttcttttact
     9181 tctttggttg ctacatcttc tccaaaccat ttttggctga tttcttggaa cttgccgtcc
     9241 ttgtaaagac tagaaaaagc ttcatttatc ttcttaacca agtttgtatc ttccttacgg
     9301 gctccaaccg caaaagcttc tgtttctagt ccaactgtaa agacattata atcgtttaaa
     9361 acaccttctg cttctaaata atagtttgca tagacacggt caatcaatag accatcaatt
     9421 cgatcgtttt tcaaatcaat caaggcttca ttaaaggttt ggtattgatt cgcttcctta
     9481 ttagcgacaa tattcttcaa aagttctgga tttgcttcaa agtccgcata accagatgaa
     9541 ccagcttgag ctcctaatgt ctttccagtc atatcctttg cagtcgtgat accagatgat
     9601 ttcttcgtaa ccaatacctg ctcattcttc atatatgagt tactgaaagc caccttttca
     9661 cggcgttcgt ctgtagcgga atagccattc caaatcagat caatcgttcc ttttgtcaat
     9721 tcagcttctt tcaaatccca atcaatcggt tgccaattta ccgtgattcc gtatttttca
     9781 aaaacagctg tagctaaatc aatatcaaat cctgcataag aaccatcttt ctgagcaaat
     9841 cccattggaa caaaagtact atcaaatcca atagtaatag acttgttaga ctggtacttt
     9901 gaccaattat ctccactagt ttcgctagaa tttttcccac aagctactaa gaacaaagca
     9961 gtcatcagac tgactaatac aagcatccat tttttcatcc atttttcctc ctatttaggt
    10021 tctactttca ataatacatc tgcgatattt tcagcaaact gcaaatcatg ggtaaccaca
    10081 atctgggtca tcccaagttc cctattttgc aagattagct tctccacttc caaacgtaat
    10141 tctggatcca gggcagaagt tggttcatcg tagccaatga tttctgggtc aatcatcata
    10201 gcacgcgcca aagccactcg ctgcttttgc ccaccagata gtgagaaagg ataggcctct
    10261 gcgtgtcctc ctagtcctaa ctgttccaag agtccactcg ccttcttctc agcctcttcc
    10321 tgcttcattc ccatggtctt cacaggcgat aaagtcaaat tttccagaac tgatagatga
    10381 ggaaatagtt gaaaatcttg gaagacaaat cccagtaggt tgcgcttctg caattcatcc
    10441 agctctaaag gttgtccatt ataaaagatt tgccctgaat caatggtttc aagacctgca
    10501 agcatacgta aaagagttgt cttacctcca ccagaaggtc caacgatagc caggatttgc
    10561 ttttcaggaa tacttagact gaaattagac aggatttgtt tgtctccaaa gactttattg
    10621 atatttcgta attctaacat ggcagcctcc tatctataat aactatactt cttctcaact
    10681 tttttggaaa taattgtcac aatcccaatc aaaatcaaat aaatggctcc tgccaagaac
    10741 ataggaacta gactagcatc gcggttagca gctgttcgac tagccaagat aaggtctgaa
    10801 attccgagag catagaccaa agaagtatcc ttgaccaaac tcataacttc attaaagaca
    10861 ctaggaagaa caatcttggt cacttggggc aagataatat agcgcactct gtcaaaaggg
    10921 ctaaacttta agaccttggc agcctcatac tgtcctcttg gaatagtgtc aattccccca
    10981 cggaaaattt ctgcaaaata agctgcatag ttgagaacaa aggcaataat agctgcagga
    11041 aggcggtcta aacgaatccc aatacttggg agcacataat agataaaaat cagttgcaag
    11101 agtaaggggg ttcctcgcat aacccagatg taaatgttaa tcagataatg gaggggcttc
    11161 caatggactt gcaaggcaaa ggcaatcaaa acgcccaagg gaatcgaaaa tagcaagacc
    11221 agtgcaaaga cctgtacagt cgtgctcgca ccattcagta aactcggtaa tatctcaaat
    11281 aaataagaca tactgcacct cctaaaagta attgttccta ttatagcatg aataaacaag
    11341 attaccaagt atttttgtaa aaagattcta ccatatacaa ataaaaaccg tagctatcaa
    11401 aagagataaa ctacagttct atatgtttaa acattcttac aatctctcaa acagttcttg
    11461 catttgcaca tcatctggaa ccagttttaa gtaagtgtga gcatggactt ttgcttcttc
    11521 aaaatgtccc aattcacgca agagatagat atagtgttcc agaaattctg gattgtcctt
    11581 caaatctcct gtcaactctt gataatactc ataagcagta tccaaatcgt ccatttcttg
    11641 ataagaacga gcaatcatcc acttggtcaa aagattttct ggctcctcac tctgcaattc
    11701 tagaatatcc tcataacgct cctgctccag ataaatagtg gctaaacgaa gcaagatttc
    11761 ttctgtatcc tcagcgtctt cttttgcagt aaggagataa ttttctgcac cactagcatc
    11821 atacaattca taagaaaatt gtgaagcagc tagcaagagg cgagtttcaa agggattttt
    11881 ctctaatcct tgcttagcga tacgcagggc ttcttgaact tgatgttcct tatgtaaagc
    11941 ctgactgtac ccatactcat agccttcaaa gtcaggagaa atggtatcaa gctgcttaaa
    12001 gtagagggtg gctttttgat attcttcttg atcaaaataa agactggcca actcaaaagc
    12061 tgttaagtca tcgtattcta actccagggc tttttctaaa aactcagtag ccgtttcaaa
    12121 tttccctaac tgagcatagg caaagccaat tcgttgatag gtggaaatgc ccgtttgctc
    12181 ataaatcgag cgattatcta actgggcata ggcttgaata gccgcttggt aattttccaa
    12241 ctcactatcc aactctgcca aacccaatat caagagagaa tcctctgagt aggtcaaggc
    12301 ctccaataat ttctcacgtg ccacatctgt caaaccttcc agctggtaaa ggtctgcctt
    12361 cagagccaaa gacgagacat accagtcact gtcagcttgg atttcctcaa gataggtaaa
    12421 ggcttcttct atttgaccat cctcgctagc aattgcagct agattaagat gaacctctgg
    12481 aaaatcctct acaattttca ggtaaatttc cttggcctga ggatagaaac caatcccttc
    12541 aagataagtt gccaattcat acagaagatc acttgaatca ttttctaaag ctttggcgaa
    12601 ataatgctca gccttagtta aatcttgctc ctccaaagcc tgtaacatct gttgactatt
    12661 gttcactctt gacttcctca ccctctaatt catatagacc actgactacc ttataccatt
    12721 cgaaaatggc tgaaatgaca accttagcag aggcataaac cggaatacca agtaaaactc
    12781 cccagatacc aaacatagat cctgaagtta acaaaacaaa gagaacatta atagggtgga
    12841 tgtttaattg acttcccaaa atcaatggag agacaaaacg gccttcaata gtttgttcta
    12901 cgataaagac aatcactact ttcaaaagca tgactggacc agcaatcaaa cccaatacta
    12961 gagcaggaag catggctaga aagctaccaa gataagggac cagatttaaa ataccagcag
    13021 taacccccag cgtaaccgca tagcgtagac caataatctt gaagaagatg ataaacatta
    13081 ctgctacaat aatagccact gtcacttgcc ctcgaacata gttggacaac tgttgattca
    13141 catctgataa aacttgtcca acaggttcct tcaattttct tggaatgaat tgggtcaaat
    13201 agttacgcaa gcctttccca tcacgcaaga gataaaagag catgaaagga acgataatca
    13261 aggcaacaat cacttgagaa gccccgctaa taaaggcact cacccagttg actgcctgag
    13321 atgaaacctt acttgccaaa actgtagcct ggctagaaaa attggtcaaa acttgctcta
    13381 attgaggtct gaaatcatct ggcaggtgct gggctaccaa tccattaaca atcctatcta
    13441 tatcttctaa gtaaacagga acgtttcttg caaaggtcaa aacctgacgt tgcagatttg
    13501 gaatggcgac tgccaagccc caaatgataa agagagcgat gataacaaag acaatagtga
    13561 tagctataac acgattaacc ttatgcttct ccatccaatc aacaatagga ttcaacaaat
    13621 aatataacaa accagacaaa atgactggca acatcacaac tgctaaaaag tctaaaacag
    13681 gtgaaaatag aaaactaatc ttacttaaaa taaaaagatt cagtcccaat aataaggtta
    13741 ctaaaaatac cgtaattgcc ttgttatcta aaaaccactt gaaaaaccaa gataggctaa
    13801 aatgtttctc tttttgctcc ataaatactt cctttctatt gttctctcat tataccattt
    13861 ttgaaacaaa ataactcttt tttaaagtgc ttacatctaa ttggatatct ttaatttact
    13921 tgtttttgtg atataatagt cttatcttta cacagaggta tagtaatgaa agaaactgtt
    13981 tattttggaa cttatacacg tcgtacttct caagggattt acaaggcaga ctttgataca
    14041 gaaactggtc agctttcaaa tctagaactt tttgcagctg agccaagtcc aacctacctt
    14101 gcctttgacc agcaccaaca tttatacact gttggtagcc aagacgataa ggggggaatt
    14161 gcagcctatc aaactgacgg gactgtgtta aatcatgttg ttgaagaagg agctccccac
    14221 tgttatgttg ctgtcgatga aaagcgtgat ttggtttacg cagccaacta tcacaaggga
    14281 caagtccttg tttataaacg ccaggaagat ggtagtcttc tacttagtga tatggatcaa
    14341 cacagtggcc aaggtccaca tgaaaatcaa gcttcccccc atgttcacta tacagattta
    14401 acacctgacc actatctagt gacctgcgac ttgggaactg accaagtcat cacctatgac
    14461 ctcgatcaag aaggaaaatt atctaagctc tatacctatc acagcaagcc aggagcaggc
    14521 tcacgccata tcattttcca taaccactat aaaatcgctt atctcatttg tgaactcaat
    14581 agtactatcg aagttttaat ctacgatggc gttggcgaat ttgaacgtat gcaggttatt
    14641 tcaactttac cagaagctta cgaaggcttt aatggaaccg ctgctattca tctctctaaa
    14701 gacggtaaat acctctacgc ttctaaccgt ggccatgatt ctatcgcagt atataccatc
    14761 cttgcggacg gtagcttaga gttattagaa atcgttccaa cgcatggtca gactccacgt
    14821 gattttgatt tgacacccga ccaaaaattt ctcattgttg tccatcaaga ctctgacaat
    14881 gcaactgtct ttaaacgtaa ttgtgacaat ggtcgtctag cagaactctc caacgacttc
    14941 catgttcccg aagcagtctg catccgtttt gctccttaaa acaccataaa aaatgaactt
    15001 ggtattttca agttcatttt tt
//
LOCUS       DAVMGZ010000036        14893 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000036 DAVMGZ010000000
VERSION     DAVMGZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.037"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            89..1264
                     /gene="mutY"
                     /locus_tag="VYQ93_000939"
     CDS             89..1264
                     /gene="mutY"
                     /locus_tag="VYQ93_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV3335648.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1319..2023
                     /locus_tag="VYQ93_000940"
     CDS             1319..2023
                     /locus_tag="VYQ93_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3335649.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2016..3365
                     /gene="vicK"
                     /locus_tag="VYQ93_000941"
     CDS             2016..3365
                     /gene="vicK"
                     /locus_tag="VYQ93_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV3335650.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3367..4176
                     /locus_tag="VYQ93_000942"
     CDS             3367..4176
                     /locus_tag="VYQ93_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV3335651.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4267..4509
                     /locus_tag="VYQ93_000943"
     CDS             4267..4509
                     /locus_tag="VYQ93_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV3335652.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMIHDLGLKDE"
     gene            4511..4765
                     /locus_tag="VYQ93_000944"
     CDS             4511..4765
                     /locus_tag="VYQ93_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV3335653.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4840..6726
                     /locus_tag="VYQ93_000945"
     CDS             4840..6726
                     /locus_tag="VYQ93_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEV3335654.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKGGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFETFSDLVYPQGIYRFSNKLFSDYPNAEKLQGKGTKNKIV
                     SKSLTQLDFVFKNNEEKDHVALLGRIGSERVYRYINKKYLDLPETFENYNVFVPEANG
                     SGALGEVLSTPLIGEPLIGEPLIGHTDTFLSIGNFKTKFEADACIKFIKTKFARVLLG
                     VLKVTQHNSRKTWYYVPLQDFTVNSDIDWTQSVTDIDRQLDQKYDLSPEEIAFIENHV
                     REMD"
     gene            6891..10145
                     /locus_tag="VYQ93_000946"
     CDS             6891..10145
                     /locus_tag="VYQ93_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV3335655.1"
                     /translation="MPVEIKTTKEIHPKIYAYTTPTVTSNEGWIKIGYTERDVTQRIK
                     EQTHTAHIATDVLWTGDAAYTEEPDKGKTFKDHDFHHFLSFHDVERRPKTEWFYFNGT
                     PEKSKNLFDKFVQHDLSGYQPGKGQDYTLRQEQEEAVAKTLAYFQEHAGGKFLWNAKP
                     RFGKTLSTYDLARRMEAVNVLIVTNRPAIANSWYDDFETFIAGQTTYKFVSESDSLKS
                     RPILSRQEFLGILADDVRQLAFISLQDLKGSVYLGGEHDKLKWVTDLHWDLLVIDEAH
                     EGVDTFKTDQAFNKIRRNFTLHLSGTSFKALAKGDFTEEQIYNWSYADEQAAKYSWSL
                     EQEEENPYESLPQLNLFTYQMSQMIGEKLEKGAQIDGENIDYVFDLSEFFATDDKGKF
                     IHEHDVRNWLDTLSSNEKYPFSTKELRNELKHTFWLLERVASAKALKALLEEHPIYEN
                     YEIVLAAGDGRMSEEDDKVKLKSLDLVRKAIAENDKTITLSVGQLTTGVTIPEWTGVL
                     MLSNLKSPALYMQAAFRAQNPYSWSDNKGNHFRKERAYVFDFAPERTLILFDEFANNL
                     SLVTAAGRGTSATREENIRELLNFFPIIAEDRAGKMVEIDAKAVLTTPRQIKAREVLK
                     RGFMSNLLFDNISGIFQASQTVLDILNELPVEKEGKVQDSSDLLDFSDVTVDDEGNAV
                     VDHEIVVNQQMRLFGEKVYGLGESVAELVTKDEERTQKQLVNDLSKTVSSVIVEELKA
                     DYSLKTRETEQIKKQITATLENEIRKNDIERKISEAHIKQELQQQLKEANDKAQKDKI
                     QEDLEKRLEENKLIHKEKLEQTLKKEVEKMPEKFIEQVEIKRVEQLKQSAQDEIRDHL
                     RGFARTIPSFIMAYGDQTLTLDNFDAFVPEHVFYEVTGITIDQFRYLRDGGQDFAGHL
                     FDKATFDEAIQEFLRKKKELADYFKDQKEDIFDYIPPQKTNQIFTPKRVVKRMVDDLE
                     KENPGIFDDPSKTFIDLYMKSGLYIAELVKRLYNSNGLKEAFPNPEERLKHILEKQVY
                     GFAPSEIIYNISTNFIFGNLSKDISRKNFVLADTIPAAKEGSIQKLVDSYFENN"
     gene            complement(10531..11517)
                     /locus_tag="VYQ93_000947"
     CDS             complement(10531..11517)
                     /locus_tag="VYQ93_000947"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV3335656.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            11711..14179
                     /gene="gyrA"
                     /locus_tag="VYQ93_000948"
     CDS             11711..14179
                     /gene="gyrA"
                     /locus_tag="VYQ93_000948"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV3335657.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            14179..>14893
                     /gene="srtA"
                     /locus_tag="VYQ93_000949"
     CDS             14179..>14893
                     /gene="srtA"
                     /locus_tag="VYQ93_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV3335658.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTA"
BASE COUNT         4781 a         2527 c         3252 g         4333 t
ORIGIN      
        1 agtagataga aaagttatat actatctatt gaaaatatta taaatcagat acaaagacag
       61 gtgaaacctt ctgtgttata ctagagatat gttagatttg aaagaatacg gtatcgtcat
      121 gtggccggag gagaaagtca tttctttccg tgagaaactt ctcgcttggt atgatgaaaa
      181 caaaagagat ttgccttgga ggagaagtaa aaatccttat cacatctggg tatctgaaat
      241 catgcttcag cagaccaggg tggatacagt tatcccttac tacgaaagat ttttggactg
      301 gtttccaact gtcgaaagtc tggcaactgc gcctgaggag agtttactga aagcttggga
      361 gggcttgggc tattattctc gagttcgcaa tatgcaggct gcagcccagc agattatgac
      421 tgactttggt ggccaatttc caaataccta tgaaggaatt tccagcttga aagggattgg
      481 accttacaca gcaggagcca tttccagtat tgcttttaac ttgcctgagc cagctgtaga
      541 tggtaatgtc atgcgggtct tggcgcgtct gtttgaagtc aaccacgata ttgggattcc
      601 aagtaatcga aaaatttttc aggcaatgat ggagatcttg attaatccag atcgtccggg
      661 tgattttaat caagccttga tggacttagg gtctgatatt gagtctcctg taaatcccag
      721 acctgaagaa agcccagtca aggactttag tgcggcatat cagaatggaa ccatggaccg
      781 ttatccaatt aaatctccta agaaaaagcc ggttcctatt tatcttaaag ccttagttgt
      841 gaaaaattct caaggacaat ttttacttga aaaaaatgaa agcgaaaagc tcttggcagg
      901 tttttggcat ttccccttta tagaagttga taacttttcg caagaagagc agtttgacct
      961 ctttcatcag gttgcggaag aaagtgtgaa ctctggcccc agtccagaag agagtttcca
     1021 gcaggactat gacctagatg ttgattggct tgatgtttgt tttgatacag tccagcatgt
     1081 ttttagtcat cgaaaatggc atgtacagat tgtagcaggt caggtgagtg acttccatga
     1141 tttttcagat agggaagttc gttggctttc accagaagaa ttcaagaatt acccacttgc
     1201 caaaccccaa caaaaaatct ggcaggctta tgcacaagcc aacttagaca gtagccaaga
     1261 ctagctattg tgtcttctga ctattttttg gtataatagt agagaaaaag gtgaacatat
     1321 gaaaaaaata ctaattgtag atgatgagaa accaatctcg gatattatca agtttaatat
     1381 gaccaaggaa ggttacgaag ttgtaactgc ttttaatggt cgtgaagcgc tagagcaatt
     1441 tgaagcagag caaccagata ttattattct ggatttgatg cttccagaaa ttgatggttt
     1501 agaagttgct aagaccattc gtaagacaag cagtgtgccc attcttatgc tttcagccaa
     1561 agatagtgaa tttgataagg ttatcggttt ggaacttggg gcagatgact atgtaacgaa
     1621 acccttctcc aatcgtgagt tgcaggcgcg tgttaaagct cttctgcgtc gttctcaacc
     1681 tatgccagta gatggtcagg aagcagatag taaacctcaa cctatccaaa ttggggattt
     1741 agaaattgtt ccagacgcct acgtggctaa aaaatatggc gaagaactag acttaaccca
     1801 tcgtgaattt gagcttttgt atcatttagc atcgcataca ggtcaagtca tcacgcgcga
     1861 acacttgctt gagactgtct ggggttatga ctattttggt gatgtccgta cagttgatgt
     1921 gactgtacga cgtctgcgtg agaagattga agatacgccc agccgaccag agtatatctt
     1981 gacgcgccgt ggtgtagggt attacatgag aaataatgct tgatttactg aaacaaacca
     2041 tttttaccag agattttatc tttatcctga ttttgttagg tttcatcctt gttgtgaccc
     2101 tcttattact ggaaaataga cgtgataata ttcagttgaa gcaaatcaat caaaaggtta
     2161 aagatttgat tgcaggagat tattccaagg ttcttgatat gcaaggtggg tctgaaatca
     2221 ccaatattac caataatttg aatgacttgt cggaggttat tcgtctcact caggaaaatc
     2281 tagaacaaga gagtaagagg ctaaatagta ttctgtttta tatgacagat ggggttcttg
     2341 cgaccaaccg tcggggtcag attatcatga ttaacgatac agccaagaag caactggggt
     2401 tggttaagga agatgttctg aatagaagca ttttggaatt gctcaagata gaagaaaact
     2461 atgaattgcg tgatttgatt acccaaagtc cagaattgtt gctagattcc caagatatca
     2521 atggcgaata tttgaacctt cgagttcgct ttgccttgat acgtcgagag tctggcttta
     2581 tttcaggttt ggtggctgtt ttgcatgata cgacggagca ggagaaggaa gaacgcgaac
     2641 gaagactctt tgtttccaat gttagccatg agttacggac tcctctgact agcgtaaaat
     2701 cctatcttga agccttggat gagggggctt tgtgtgaaac tgtagcacca gactttatca
     2761 aggtttctct tgatgagacc aaccgtatga tgcgcatggt gacggatctc ctccatcttt
     2821 cacgtattga taatgctacc agtcacctag atgtggaact gattaacttc actgctttta
     2881 ttacctttat cctcaatcgt tttgacaaga tgaaaggaca ggaaaaggag aaaaaatatg
     2941 agttggtgag agattatccc atcaattcta tctggatgga aattgataca gataagatga
     3001 cgcaggttgt cgacaatatt ttaaataatg ctattaagta ttcgccagat gggggtaaaa
     3061 tcactgtcag aatgaagaca actgaagacc agatgatttt atccatttct gaccacggtt
     3121 tggggattcc taagcaggat ttaccacgta tctttgaccg tttctatcgt gtggatcgtg
     3181 ctagaagtcg tgcacaaggt ggtacaggtc taggactgtc tatcgctaaa gaaattatca
     3241 aacaacataa gggctttatt tgggccaaga gtgaatacgg caagggttca acctttacca
     3301 ttgtgctccc ttatgataag gatgcagtga aagaagaagt atgggaggat gaagtagaag
     3361 actagaatga gtgaaatagg ctttaaatac agtattttag cgtcgggttc cagtggaaat
     3421 tctttttatc tggaaacctc aaaaaagaag cttttagtag atgcaggctt gtctggcaag
     3481 aaaattacca gtctgctagc tgaaattaac cgtaagccag aagacctgga tgccatcttg
     3541 attacccatg agcattcaga tcatatccat ggagtaggcg ttttggctcg caagtatggt
     3601 atggatcttt atgccaatga aaagacctgg caagctatgg aaaatagtaa atatcttggc
     3661 aaggtggatt cttcgcaaaa gcatattttt gaaatgggta aaaccaaaac ctttggagat
     3721 atcgacatcg agagttttgg tgtaagccat gatgcagtcg caccgcagtt ctatcgcttt
     3781 atgaaggatg ataagagttt tgtcctcttg acagatacag gttatgtcag tgaccgtatg
     3841 gcgggcattg tcgaaaatgc ggatggttat cttatcgagg ccaaccatga tgtagagatt
     3901 ttgcgatcag gttcttacgc ttggcgcctc aaacaacgaa tcctatctga ccttggtcac
     3961 ctttctaacg aggacggtgc tgaagctatg attcggacgc taggaaatcg tactaagaag
     4021 atttaccttg ggcatttatc taaggaaaac aatatcaagg aactggctca tatgaccatg
     4081 gtcaatcagc tggctcaagc tgatctggga gtcggagtag actttaaggt ttatgatacc
     4141 tcaccagata ccgcaacacc attgacagag atatagaaag aacgctgaga aggtgttctt
     4201 tttatattga ctggacacct aaaaagtaat acaatggtgt taccattaaa aaagggagca
     4261 caaaagatga ctactattac attaaaagtt tctgaagctg ataaaacatt tatgaaagca
     4321 atggctaagt ttgaaggagt ttccctttcg gaacttattc gcaccaaaac tcttgaagcc
     4381 ctagaagatg aatacgatgc tcgtgtggca gatttagcct atcaagagta tttagaagac
     4441 ttggaaaaag gagttgaacc cattacttgg gaagaaatga tccatgattt aggcttgaag
     4501 gatgaataat ttgtataaat tagttccaac aagacgtttt atcaagcaat tgaaaaaatt
     4561 ggaccgttat acgcagaagc taattacaaa ctatttacaa accaatgttt tggaagaccc
     4621 aagacgacac ggaaaggctt tggttggtaa tcgcgttggt caatggcgct atagaattgg
     4681 taattatcga gttatcgtac aaattgtaga tgatgaatta gtcgttgcta ctctagaagt
     4741 tggtcatcgg agagatattt attgaattac ttttttcaag taaattttca ttctttaaaa
     4801 tatgatataa tagggcttac aatataagag aggttgctga tgacaataga tattagtgaa
     4861 gaaagtttgg ctaaagaatc ggctgactta ttaaaaattc ttttaaaaga tcgaacgaca
     4921 aagaaatcaa ttgtttgggc tacgcattct tatgaattgt taggaaaggg atttgctcca
     4981 agtgatcgca tcaatccaag taaggttact ggaaactttg ccaacttgat tcagcccaga
     5041 tctgaaaaat ccaaatatga acaaaaagat cggacaaaaa taagggcaga agtgtttaca
     5101 ccaacgtggt tggtcgcaaa gcaaaatgga tatgtagaat cgaaattagg ctcgttaagt
     5161 cttgaggagt atgtggactt acgatggtta gaggtaactt gtggtgaagc accttatatg
     5221 gtaacgagat atgataccgt aactggtgag gaaattccat tatctgagcg agttggtttt
     5281 gttgatagaa aactgcaacg gattagtcgt gaggtttcag atgaagtcac attttataaa
     5341 cttgtaaaag atgcttatca tgcttcgtat ggttatgagt atcaaggaga ttctctcctt
     5401 ttggcgcgtg agaatctttt ggcaactttt gaggactatt atctagctaa gactggaaat
     5461 caaccaacat tagagcaaaa gaaagaaatt gcgactatta tctcttacaa tgtctttcag
     5521 atggatggat tgaaaaaaag tagtccttat tcagctaaac aaaagcaatc tcaacaattg
     5581 agtttgtttg cggatgagct agaagtccaa gaagtagaag aatctaaaac ccatatcaaa
     5641 gactggaaaa agaatagaat gataggtttt gagcgcctat ctagcgagga aagtgaaatg
     5701 aaatttgatg tcgtgatagg aaatcctccg tatcaggaaa caggagaagc cagagatgag
     5761 ccgatatatc actattttat tgatgaagct tataaaatcg cagataaatc tatactaatc
     5821 accccagcaa ggtttttatt taaagctggt caaactccta aaaattggat ggaaaagatg
     5881 cttgaagaca agcatttaaa agttcaattc tatgaactta aaagcggtaa agtatttaca
     5941 ggaacggata ttaagggtgg agtagcaata acttatcgtg atgttgataa agatttggga
     6001 ccaatcggag tatttactat ttttgaaact ttgaatggta ttgtaaaaaa aatcgaaaaa
     6061 catccagaat ttgaaacttt ttctgattta gtttatcctc aaggtattta tagattttca
     6121 aataaacttt tttctgatta tccaaatgct gaaaagctgc aaggaaaagg gacaaaaaat
     6181 aaaattgttt cgaaatcact aactcagtta gattttgttt tcaaaaacaa cgaagaaaaa
     6241 gaccatgttg ctctacttgg aagaattggc tccgaacgtg tttatcgata tattaataaa
     6301 aaatatttag atttaccgga aacattcgaa aattataatg tttttgtacc agaagctaat
     6361 ggaagtggtg ccttaggtga agtcttatca acacccctaa tcggggaacc cctaatcggg
     6421 gaacccctaa tcgggcatac agatactttt ttatctattg gtaattttaa aacaaaattt
     6481 gaagccgatg cttgtattaa atttattaaa actaaattcg ctagagtatt attaggtgtt
     6541 ttgaaagtta ctcagcataa ttcacgcaaa acttggtatt acgtccccct ccaagacttt
     6601 acggtcaatt cggacattga ttggacacaa tcagtgactg atattgaccg ccagcttgat
     6661 caaaaatatg acctttcccc tgaagaaatt gcctttattg agaatcatgt aagggagatg
     6721 gattagaaaa gtatttttat ttgacaaata gtgctcaatg atctaaaatg actatatagg
     6781 attaggtcag gaagcatacg atgccctgac cctttttgta cttatgagat gagaaagtca
     6841 tttgttagat aaattgactt cgttagcaaa cgttcaaaaa aggaaaactt atgccagtag
     6901 aaattaaaac cactaaagaa attcatccta aaatctatgc ctacaccaca ccgacagtaa
     6961 ccagtaatga aggctggatt aagattgggt atacagaacg tgatgtcaca caacgtatca
     7021 aggagcaaac gcatacagct catatagcta cagatgtctt atggactggt gatgcagctt
     7081 atacagaaga gcctgataag gggaaaactt tcaaggacca tgatttccac catttccttt
     7141 ctttccatga tgtagaacgt cgtcccaaga cggaatggtt ctattttaat ggaactcctg
     7201 aaaaatcaaa aaatcttttt gataagtttg ttcagcatga tttgtctggt tatcagcctg
     7261 gaaaaggaca ggactatact ctgcgacaag agcaagaaga agcagttgct aagacattag
     7321 cttatttcca agaacatgct ggaggcaagt ttctctggaa tgccaagcca cgctttggta
     7381 aaaccttgtc tacctatgac ctagctcgac ggatggaagc tgtcaatgtc ctaattgtaa
     7441 caaaccgccc tgccattgct aactcatggt atgatgattt tgaaacattc atagcaggtc
     7501 aaacgactta caagtttgtt tctgaatcag atagccttaa gagtcgtcca atcttgtcac
     7561 gacaagaatt tcttggtatt ttagctgacg atgtaagaca acttgctttt atcagtctcc
     7621 aagacttgaa aggatctgtt tatttaggtg gagagcacga taaactcaaa tgggtaactg
     7681 atctgcattg ggacttgttg gttattgacg aggctcatga aggagttgat accttcaaga
     7741 ctgaccaagc ctttaataag attcgacgaa attttactct gcatttgtca ggtacatcat
     7801 ttaaagcatt ggctaaagga gattttacag aggaacaaat ctacaactgg tcttatgctg
     7861 atgagcaggc tgctaagtat tcgtggtctc ttgagcaaga agaggaaaat ccttatgaaa
     7921 gcttgcctca gttgaatctc tttacctatc aaatgtctca gatgattggc gaaaagttag
     7981 aaaaaggcgc tcagatcgat ggtgaaaata ttgactatgt ttttgactta agtgaatttt
     8041 tcgctacaga tgataaaggg aaatttattc atgagcatga tgtcagaaat tggttagata
     8101 ctctatcaag caatgaaaaa tatccatttt caaccaaaga actccgtaat gaactcaagc
     8161 atactttttg gcttttagaa cgtgtcgctt cggccaaagc attaaaagcc ctactagaag
     8221 aacacccaat ctatgaaaac tatgagatcg ttctagctgc tggtgacgga cgtatgtccg
     8281 aagaagacga taaagtcaaa ctcaaatcct tggacttggt tagaaaagcg atagcagaga
     8341 atgacaaaac cattacccta tccgttggtc agctgacgac aggtgtcact atccctgaat
     8401 ggacaggtgt attgatgtta tcaaatttga aatcaccagc tctttatatg caggccgcct
     8461 tccgtgctca aaatccttac tcatggagcg ataacaaagg aaatcacttt cgcaaagaaa
     8521 gagcctatgt atttgacttt gcgccggaaa gaaccttgat tctctttgat gagtttgcca
     8581 acaacttatc gcttgtaact gcagctggta gaggaacttc agctacacgc gaagaaaata
     8641 ttagagaatt attaaacttc tttccaatta ttgccgaaga ccgtgctggt aagatggttg
     8701 aaattgatgc aaaggcagtt ctaaccactc ctcgccagat aaaagctaga gaagttctta
     8761 aacgaggttt tatgtccaat ctcttatttg ataatattag tggtattttc caagcaagtc
     8821 aaacagtttt agatatttta aatgagctgc cagttgaaaa ggaagggaag gtacaagata
     8881 gttctgattt attagatttt tcagatgtta cagtcgatga tgagggaaat gcagtagtag
     8941 accatgaaat tgtagttaat cagcaaatgc gactttttgg tgaaaaagtt tatggacttg
     9001 gtgaatctgt tgctgagtta gtcacaaaag atgaggaacg aactcaaaaa cagctggtca
     9061 atgacttgag taagaccgtt tcttcagtga ttgtagagga attgaaagca gattattctc
     9121 taaaaacaag ggaaactgag caaattaaga aacaaattac agcaacactt gagaatgaaa
     9181 ttcgaaaaaa tgatatcgaa agaaaaattt ctgaagctca tatcaagcaa gagttgcaac
     9241 agcagctcaa agaagcaaat gataaagcgc aaaaagataa gattcaagaa gatttggaaa
     9301 aacgtttaga agaaaataaa ctcattcata aagaaaaact agaacaaaca ctcaaaaaag
     9361 aagtggaaaa aatgcctgag aaatttatcg aacaggttga gataaaacgt gtggaacagt
     9421 tgaaacaatc agctcaagat gaaattcgtg accatttacg agggtttgca agaacaattc
     9481 caagttttat tatggcttac ggtgatcaaa ctctaacact tgataatttt gatgcctttg
     9541 ttcctgaaca tgttttttat gaagtaacag ggattacgat tgatcagttt agatatttgc
     9601 gagatggtgg gcaggatttt gcagggcatc tctttgataa agcaacattt gacgaagcta
     9661 ttcaagaatt tcttcgcaag aaaaaggagt tggcggatta ttttaaagat caaaaagaag
     9721 acatttttga ctatattcca ccgcagaaga ccaaccaaat tttcactcct aaacgagtgg
     9781 tgaaaaggat ggtagatgat ttggaaaagg aaaatccagg gatttttgat gatccatcta
     9841 agacttttat tgatttatat atgaagtcag gcctctatat tgcagaactt gtgaagcggt
     9901 tatataatag caatggcttg aaagaggcct ttccaaatcc tgaagaacgc ttaaaacata
     9961 ttttggaaaa gcaagtttat ggatttgctc cgtctgagat tatctataac atttccacta
    10021 attttatatt tggtaatctt tctaaagata tcagtaggaa gaattttgtt ttagcagata
    10081 ccattccagc ggctaaagaa gggagcattc aaaagttggt tgattcctat tttgaaaata
    10141 attaaaaaga aggccgagtc aaaattcttt gaaatcagaa aaaacgcata atattgagtg
    10201 cttttgtact gccccccaaa agttagacag aaaaaatcta acttttgggg ggcagttcag
    10261 acaatccttg gtattatgcg ttttattgtg ggaagatgta taatggattg aaataagata
    10321 tgaacaaatc gattaggaaa ggaaaattga tttatagaaa tgttttagta gtcgatgcgt
    10381 actgttatag attcaacgaa ctataaaatc tgaagaaagc aattttaacc agatcttaga
    10441 agcagttgtg tactactcta acttcaatag actatatcat atagataaaa aacaactccc
    10501 tgatgattca aggagttgtc tatagttaaa ttagttttta gaagcttctt ggaattctgg
    10561 atttttccat gcttcgtcaa taatagcttg caattcttta gcagatgctt gcattttttg
    10621 agtttctgca tcgttcaatg gaatgtttac tggacgaacg ataccatgtg caccaacaac
    10681 agctggttga ccgataaaga cattctcaac tccgtattga ccttcttgga atactgaaag
    10741 tggaagtact gcgttttcat cgtcaaggat tgctttagtg atacgagcaa gggctactgc
    10801 gataccgtag tatgttgcac cttttttgtt gatgattgtg taggctgcat cacgaacacc
    10861 ttcgaacaat tcaatcaatt cagcttcttg aacattttga gtgtctttaa ggaattcttc
    10921 aaggtttaca ccagcgatgt tagcgtgtga ccaaacagcg aactcagagt caccgtgttc
    10981 acccatgatg taggcgtgca ctgaacgagc atccacatcc aatttttcag caagtgcttg
    11041 acggaaacga gctgagtcaa gtgaagtacc tgaaccgata acgcgttctt tagggaaacc
    11101 agagaatttc caagttgagt aagtcaaaac gtcaactgga ttagcagcaa caaggaagat
    11161 acctttgaaa ccagattcaa caacttgagt tacgattgat ttgttgatag caaggttttt
    11221 acctacaagg tcaagacgag tttcacctgg tttttgaggt gcacctgcag tgatcacaac
    11281 aaggtcagcg tctgcacagt cagagtattg agctgcatag atttttttag gtgaagtgaa
    11341 ggcaagggcg tgactaaggt caagcgcatc accaacagct ttttcatgca attgtggaat
    11401 ttcgataatt ccaagctctt gtgcaattcc ttggttaaca agtgcaaaag cgtaagatga
    11461 acctacagca ccatcaccga caaggataac ttttttgtgt tgtttagttg aagtcattgt
    11521 tttaaacatc tccttaattt tattagggga ttttccctag acaacttcat tctatcactt
    11581 ttaaaaaact ttgtcacgaa tatgccttat agttctcgat gtaaacgttt tagtggttta
    11641 gaggctgaaa tagatgggaa tttatggtat aatgttgtta cttactaatt gtgaaatgag
    11701 gcatttatta atgcaggata aaaatttagt gaatgtcaat ctgacaaagg agatgaaggc
    11761 aagttttatc gactacgcca tgagtgttat cgtagcgcga gctcttcctg atgttcgaga
    11821 tggcttaaaa cctgttcacc gtcgcattct ctacggaatg aatgaattgg gtgtgacccc
    11881 agacaaaccc cataaaaaat ctgctcgtat tacaggggat gtcatgggta aatatcaccc
    11941 acacggggat tcctctattt atgaagccat ggtccgtatg gctcaatggt ggagctaccg
    12001 ttacatgctt gtagatggtc atgggaattt tggttccatg gatggagata gtgctgccgc
    12061 tcaacgttat accgaggcac gtatgagcaa gattgctctg gaaatgcttc gtgatatcaa
    12121 caaaaataca gttgatttcg ttgataacta tgatgccaat gaacgggaac ccttggtctt
    12181 gccagcgcgt tttccaaacc ttttggttaa tggagcaact ggtatcgcgg ttgggatggc
    12241 aaccaatatt ccacctcata atctgggtga aaccattgat gcagtgaagt tggtcatgga
    12301 taatcctgaa gtgactacca aggacttgat ggaagtcttg cctggaccag attttccaac
    12361 tggtgctctt gtcatgggga aatcaggtat ccataaggct tatgaaacag gtaaaggttc
    12421 gattgtccta cgttctcgta cagagattga aacgactaag actggtcgtg agcgcatcgt
    12481 tgtaacagaa tttccttaca tggtcaataa aaccaaggtg catgagcata ttgttcgctt
    12541 ggttcaggaa aaacgcattg agggtatcac agcagtacgt gatgagtcaa accgtgaagg
    12601 tgttcgattt gttattgaag tcaagcgcga cgcctcagcc aatgttattc tcaataacct
    12661 cttcaaaatg acccaaatgc aaaccaattt tggtttcaat atgctcgcta tccaaaatgg
    12721 tataccgaaa attttgtctc ttcgtcagat tttggatgct tatatcgagc accaaaaaga
    12781 agtggttgtt cgtcgtacac gttttgataa ggaaaaagcg gaagcgcgcg ctcatatctt
    12841 agaaggtctc ttgattgcgc tagaccatat cgacgaagtg attcgtatca tccgtgctag
    12901 tgaaacggat gcggaagctc aagctgagtt gatgagcaag tttaagcttt ctgaacgtca
    12961 aagtcaagct atccttgata tgcgtcttcg tcgtttgaca ggtttggaac gcgataagat
    13021 tcaatctgag tatgatgacc tcttggctct gattgcggat ttagcagata ttcttgctaa
    13081 gcctgaacgt gtttctcaaa ttatcaaaga cgaattggat gaagttaaac gtaaattttc
    13141 tgataaacgc cgtacagagt tgatggttgg acagattttg agtctcgagg atgaggactt
    13201 gattgaagaa tcggatgtct tgattaccct ttctaacaga ggctacatta agcgtttgga
    13261 tcaggacgag ttcactgctc aaaaacgtgg gggtcgtggt gtccaaggaa cgggagtgaa
    13321 agatgatgac tttgttcgtg agttagtgtc aactagcaca catgatcatc tgctcttctt
    13381 cacaaacaag ggacgtgtct atcgtcttaa aggttatgaa attcctgagt atggtcggac
    13441 tgccaaaggg ctaccagtag tcaatctctt gaaattggat gaagacgaaa gtattcagac
    13501 ggttatcaat gttgagtctg atcgcagtga tgatgcttat ctcttcttta caacccgtca
    13561 cggtattgtg aagagaacta gtgttaagga gtttgccaat attcgtcaaa atggtctcaa
    13621 agcgctgaat ttaaaggatg aagatgagtt aatcaatgtc ttgttggcag aaggagatat
    13681 ggatattatc attggtacca agtttggtta tgcagttcgc tttaatcaat cagccgttcg
    13741 tggtatgagc cgtatcgcca ctggtgtgaa aggtgttaac cttcgtgaag gagacacagt
    13801 tgttggtgcc agcttgatta ctgatcaaga tgaagttctt attatcacag aaaaaggata
    13861 tggtaagcgt acagtcgcta ctgaataccc aacaaaaggt cgtggtggta agggaatgca
    13921 gacagctaaa attaccgaaa aaaatggctt gctggccggt cttatgactg ttcaagggga
    13981 tgaggatttg atgattatca ctgatacagg tgtcatgatt cgaaccaatc ttgccaatat
    14041 ttcacaaaca ggacgtgcaa ctatgggagt taaagtaatg cgcctggatc aagatgctca
    14101 gatagtgact ttcacaacgg ttgcggtggc agaaaaagaa gaagttggga cagaaaacga
    14161 aacagaaggt gaagcataat gtctcaaaaa aataataaaa agaaaaacaa gcgaaaaaat
    14221 ctgctgacaa atatcctagc aggatttctg atattactgt cactggcttt gatttttaat
    14281 actcaaattc gaaatatttt catagtctgg aataccaata agtatcaagt tagccaggta
    14341 tcaaaagaaa aattagaaga aaatcaggat acagaaggca attttgactt tgattctgtc
    14401 aaagctatct cttcggaagc tgttctaact tctcaatggg atgctcaaaa attaccagtt
    14461 attgggggaa ttgcaattcc tgaattggaa atgaatttgc cgatttttaa aggacttgat
    14521 aatgttaatc tcttctacgg agctggtaca atgaaacgcg agcaagtaat gggagaagga
    14581 aattatagtc tagctagtca ccatatcttt ggtgttgata atgctaataa aatgttattt
    14641 tctcctttag ataatgctaa aaatggcatg aagatttatc taaccgataa aaataaagtt
    14701 tatacttatg aaatacgtga agtcaaacgt gtgacaccgg atcgtgttga tgaagttgat
    14761 gatagagatg gggtcaatga aatcacatta gtaacctgtg aagaccttgc tgctacagaa
    14821 cgtattattg tcaaaggtga tttgaaagaa acaaaagatt attcacaaac atctgatgaa
    14881 atcctaacag ctt
//
LOCUS       DAVMGZ010000037        14666 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000037 DAVMGZ010000000
VERSION     DAVMGZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14666
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.038"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(73..822)
                     /locus_tag="VYQ93_000950"
     CDS             complement(73..822)
                     /locus_tag="VYQ93_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV3335659.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDCSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            955..3291
                     /locus_tag="VYQ93_000951"
     CDS             955..3291
                     /locus_tag="VYQ93_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV3335660.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKLINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLLFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3393..4070
                     /locus_tag="VYQ93_000952"
     CDS             3393..4070
                     /locus_tag="VYQ93_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV3335661.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4057..4854
                     /locus_tag="VYQ93_000953"
     CDS             4057..4854
                     /locus_tag="VYQ93_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV3335662.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4871..5974
                     /locus_tag="VYQ93_000954"
     CDS             4871..5974
                     /locus_tag="VYQ93_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV3335663.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            5981..6172
                     /locus_tag="VYQ93_000955"
     CDS             5981..6172
                     /locus_tag="VYQ93_000955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335664.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            6275..7294
                     /gene="galE"
                     /locus_tag="VYQ93_000956"
     CDS             6275..7294
                     /gene="galE"
                     /locus_tag="VYQ93_000956"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV3335665.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            7296..8261
                     /locus_tag="VYQ93_000957"
     CDS             7296..8261
                     /locus_tag="VYQ93_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3335666.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(8312..8518)
                     /locus_tag="VYQ93_000958"
     CDS             complement(8312..8518)
                     /locus_tag="VYQ93_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV3335667.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            8570..9046
                     /locus_tag="VYQ93_000959"
     CDS             8570..9046
                     /locus_tag="VYQ93_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV3335668.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSHPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            9055..9726
                     /locus_tag="VYQ93_000960"
     CDS             9055..9726
                     /locus_tag="VYQ93_000960"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV3335669.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            9857..10195
                     /locus_tag="VYQ93_000961"
     CDS             9857..10195
                     /locus_tag="VYQ93_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV3335670.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            10198..10827
                     /locus_tag="VYQ93_000962"
     CDS             10198..10827
                     /locus_tag="VYQ93_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV3335671.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKYGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            11067..12218
                     /locus_tag="VYQ93_000963"
     CDS             11067..12218
                     /locus_tag="VYQ93_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3335672.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            12531..13280
                     /gene="truA"
                     /locus_tag="VYQ93_000964"
     CDS             12531..13280
                     /gene="truA"
                     /locus_tag="VYQ93_000964"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV3335673.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            13270..14043
                     /locus_tag="VYQ93_000965"
     CDS             13270..14043
                     /locus_tag="VYQ93_000965"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV3335674.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            14021..14482
                     /locus_tag="VYQ93_000966"
     CDS             14021..14482
                     /locus_tag="VYQ93_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3335675.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         4066 a         2829 c         3428 g         4343 t
ORIGIN      
        1 gaggtgtact attctagttc caatctacta tataaatgaa aaccttagct gtttggccaa
       61 ggttcatttc ttctatgcaa gttcttctct tttcttatct gggttccaga tgaagcttgc
      121 gataaagcta aagatgatgg ttaggatagc gaggataata gcaaggatga gggcagggat
      181 gcggataaac caccagagtg gatttggttt tttatcattg tgccattgtt tcgtttcttc
      241 gtccagacgt tggaattctg tttgaatacg attggcgact gtttcaatgc cttcatcatt
      301 cattttcttg atatctgaga tatcgattgg atttccaaag ttcatatcga cacgttcacg
      361 gctaatcaag cccttcaaag tcatgggacc ggtgtaggta accggcatga tacggacctt
      421 ggccattttg gcaatcagtg ctgcgccccc cttgacatcg tttgagtggc ggctaccact
      481 tggaaacatg atgagagagc agtcactttt tttgagaacg ttgataggat atttgatggc
      541 tgaggcgctg ggattttcac ggtcgatggg aaaggcgcca cacatacgaa tccaccaacc
      601 aaagatacgg ttggtaaaga gttctttttt tgccataaag atgaactgtt ttggcttggt
      661 cgcaaaggcc atataaacag gatcccacca ggtacggtga ggcgcaacta aaatataatt
      721 ttcatcttga ttaggaattt tatcagtatt atgatagtga gcattgccat tgatggacca
      781 taagagcaat acaactaatc cacgcaaata agtataaaac atgcgatctc cttcaattgt
      841 tttcttgtta ttattatacc ttatcaaagg agggctggca aacttttccc ttgactagat
      901 gcatatttgg gatgaaatta gaattctgtt aaaaaaaatg atataataga atttatggat
      961 aaaaataaga ttatgggatt aacccaaaga gaagtcaagg aaagacaggc tgagggtttg
     1021 gtcaatgact ttaccgcatc agccagtacc agcacttggc aaatcgttaa acgaaatgtc
     1081 tttacccttt ttaacgcttt gaactttgcc attgctttgg cccttgcctt tgtgcaggct
     1141 tggagcaatc tggtcttctt tgctgttatc tgctttaacg ctttttctgg gattgtgacc
     1201 gagctacgag ccaaacacat ggtggacaag ctcaatctca tgaccaagga aaaggtcaaa
     1261 accatccgtg atggtcagga agttgctctt aatcctgaag aattagtgct aggagatgtc
     1321 attcgtttgt ctgcaggaga gcagattcct agtgatgcct tggttttgga aggctttgcg
     1381 gaagtcaatg aagccatgtt aacgggagaa agtgatttgg tgcaaaagga agttgacggc
     1441 ttacttttgt caggaagttt cctagccagt gggtcagttt tatctcaagt tcaccatgtc
     1501 ggtgcagaca actatgctgc caaactcatg cttgaggcta agaccgttaa actcatcaac
     1561 tcccgtatca tgaaatcgct ggacaagttg gctggtttta ctgggaagat tatcattccc
     1621 tttggtctgg ctctcttgct ggaagccttg cttttaaaag gcctgcctct caagtcatct
     1681 gttgtaaact cgtcgacagc tcttttggga atgttgccta agggaattgc ccttttgacc
     1741 attacttcgc tcttgactgc agtgattaag ctaggtttga aaaaggtctt ggtgcaggag
     1801 atgtactctg ttgagacctt ggcgcgcgtg gatatgctct gtctggacaa gacgggtacc
     1861 atcacccaag gaaagatgca ggtggaggct gttcttccac tgacggaaac ttatggtgaa
     1921 gaggctgttg ccagcatcct gaccagctat atggcccata gtgaggataa aaatccaaca
     1981 gctcaagcca ttcgccagcg ttttgtggga gatgttgctt atcctatgat ttccaatctt
     2041 ctcttctcga gcgaccgcaa gtggggggct atggagttag aaggtttggg aatagttttc
     2101 ttaggggcac ctgagatgtt gctggattct gaagtcccag aagccaggga ggccttggag
     2161 agaggatcac gtgtcttggt cttagctctc agtcaggaga aattagacca tcacaaacca
     2221 cagaaaccat ctgatattca ggctctagcc ttgctggaaa tcttggaccc cattcgagag
     2281 ggagcagcag agacgctgga ctatctccgt tctcaggagg tgggactcaa gattatctct
     2341 ggtgacaatc cagttacggt gtccagcatt gcccagaagg ctggttttgc ggactatcac
     2401 aactatgtag attgctcaaa aatcaccgat gaggaattga tggccatggc ggaggagaca
     2461 gcgattttcg gacgtgtttc ccctcatcaa aagaaactca tcatccaaac gttgaaaaaa
     2521 gcgggtcata caacggctat gacaggggac ggggttaatg atatcttggc ccttcgtgag
     2581 gcggattgtt ctatcgtgat ggcggagggg gatccagcaa cccgtcagat tgccaatctg
     2641 gttctcttaa actcagactt taatgatgtt cctgagattc tcttcgaggg tcgtcgcgtg
     2701 gtcaataaca ttgcccacat cgccccgatt ttcttgataa agaccatcta ttccttcctg
     2761 ttagcagtca tctgtattgc cagtgcttta ctaggtcggt cagagtggat tttgattttc
     2821 cccttcattc cgatccagat taccatgatt gaccagtttg tggaaggttt cccaccattc
     2881 gttttgactt ttgagcgaaa tatcaaacct gtcgagccaa acttcctcag aagatccatg
     2941 cttcgtgccc taccaagtgc cctcatggtc gtgtttagcg ttctttttgt gaaaatcttt
     3001 ggaacgagtc aaggttggtc tgagttagaa atctcaactc tcctctatta tctcttgggg
     3061 tcaattggtt tcttatccgt atttagagcc tgcatgccat tcactctatg gcgagtcctc
     3121 ttgattgtct ggtcagtagg aggcttccta gccacagctc tcttcccaag aatccaaaaa
     3181 ctgcttgaaa tttcaacctt gacagaacaa acgttgcctg tttatggtgt catgatgttg
     3241 gtctttacag tgattttcat cctgactagt cgctatcaag ctagaaaata aagaaagact
     3301 gcaatctgtg gattgcagtt tttttaggta caagattgcc agccgaaatg tggtataata
     3361 agaggtaata gagttttgga aagtgagaga agatgatttc aaagagatta gaattggtag
     3421 cttcctttgt gtcacagggg gctattttac tagatgtggg aagtgaccat gcttatctgc
     3481 ctatcgagtt ggttgagaga ggccaaatca aaagcgctat tgcaggtgag gtggtggaag
     3541 gtccctatca gtctgcggtt aaaaatgttg aggctcacgg cctaaaggag aaaatccaag
     3601 tccgtttagc caatggcttg gcagcttttg aagagactga ccaagtgtct gtcattacca
     3661 ttgctggcat gggtggtcgt ttgattgcta ggattttaga agaaggtttg gggaagttag
     3721 ctaatgtaga acgtttgatc ctccagccca ataatcgtga agacgacttg cgtatctggc
     3781 tacaggatca tggattccag attgtagcag aaagcatctt agaagaagct ggaaagtttt
     3841 atgagatttt ggtggtggaa gcaggacaaa tgaagctatc agccagtgat gttcgctttg
     3901 gtcccttctt gtccaaagaa gtcagtccag tatttgtcca aaaatggcaa aaagaagctg
     3961 agaagctaga gttcgccctc ggacaaatcc cagaaaaaaa tctggaagaa cgtcaagttc
     4021 tagtagataa gattcaagct atcaaggagg tgctccatgt tagcaagtga agtgatccaa
     4081 gcttatgaag ccttttgccc tcaggaattt tctatggagg gagacagctg tggtctgcaa
     4141 attggcactt tagacaaggg tatccaaagg gtcatggtgg ctctcgatat tcgtgaagag
     4201 acggtggctg aagccattga aaagggtgta gacttgatta tcgtcaagca cgcgcctatc
     4261 tttcgtccta tcaaggactt gcttgccagc cgtccgcaaa atcagattta catcgacctg
     4321 attaagcatg acatcgcagt ttatgtcagc cataccaata ttgatatcgt tgaaaatggg
     4381 ctcaatgact ggttttgtca gatgctagga atcgaggaga cgacttatct gcaggaaaca
     4441 ggtccagaac gtggaattgg acgtattggg aatattcagc ctcagacatt ttgggaattg
     4501 gcccaacagg tcaagcaagt ctttgaccta gatagccttc gaatggtgca ttatcaagag
     4561 gatgatttgc agaagcctat ttcaagagta gcaatttgtg gtggaagcgg gcagtctttc
     4621 tataaggatg ctttggcaaa gggggcagat gtctatatca ctggtgatat ctactaccat
     4681 actgctcagg atatgctgtc tgatggcttg ttagcattgg atccaggtca ctatatcgaa
     4741 gtgctttttg tggaaaaaat cgcaacactc cttactcaat ggaaagagaa aaaaggctgg
     4801 gaactagaga ttttacctag tcaagcatcg accaatcctt tccgtcatat ctagttagaa
     4861 agtgaagaca atgaaaaaag ttgccattat tggagcagga attgtgggag caacagctgc
     4921 ctactacctc tcgagagaaa gtgacctaga ggtgaccgtt tttgaccatg agcaaggtca
     4981 agccaccaag gccgcagcag gaattatcag tccttggttt tccaaacgcc gtaataaagc
     5041 ctggtacaag atggcgcgct tgggggctga tttttatgtg gatttattag ctgatttaga
     5101 gaaatcagga caagaaatcg acttttacca gcgttcggga gtctttctct tgaaaaagga
     5161 tgaatccaat ttggaagaac tttatcaact ggccctccag cgcagagaag aatctccctt
     5221 gatagggcaa ttagccattc tgaaccaagc ctcagctaat gaattattcc ctggtttgca
     5281 gggatttgac cgcctgctct atgcttctgg tggagcgaga gtagatggtc acctcttagt
     5341 aactcgtttg ctggaagcca gtcaggtcaa gttggtcaaa gaaaaagtga ctctgacact
     5401 cttatcatca ggctaccaga ttggcgaaga ggtgtttgat caggttattt tggcaacggg
     5461 agcttggttg ggggacatgt tagagccttt aggttatgaa gtggatgtcc gtcctcaaaa
     5521 aggacaacta cgagattatc agcttgccca agacatggaa gattaccctg ttgtcatgcc
     5581 agaaggggag tgggatttga ttccctttgc aggtgggaaa ttatccttgg gcgctaccca
     5641 tgaaaatgac atgggatttg atttgacggt agatgaaccc ttgctccaac aaatggagga
     5701 ggcggccttg cctcattacc cagttttagc tagagtgact tcaagggctg agcgtgtggg
     5761 aatccgtgcc tataccagtg atttctctcc cttctttggg caggtgccag aattggcagg
     5821 tgtctatgca gccagtggac taggttcatc aggcctcaca actggtccta tcattggtta
     5881 ccatctagcc caactgatcc aagacaagga gttgaccttg gaccctgaaa attacccaat
     5941 tgaaaactat gtcaaacgag taaaaagcga ataatactca atgaaaatca aagaacaaac
     6001 taggaaacta gccgcgggtt actcaaagca ctgctttgag gttgtggata gaactgacga
     6061 agtcagctca aaacactgtt ttgaggttgt ggatagaact gacgaagtca gtaaccatac
     6121 ccatggcaag gcgacgttga cgtggtttga attggatttt cgaagagtat aagcatttta
     6181 ctgaaatttt atcaggtagt ttaggatggc aaatgacatt ccctgtcaaa aatggtaaaa
     6241 taagaaaaaa tgatctgaga atcgaggaaa aaagatgcaa gaaaagattt tggtaactgg
     6301 tggtgccggt tttattggaa cccacactgt tattgaattg atccaagcag gccatcaagt
     6361 tgttgtggtg gataatcttg tcaatagcaa tcgtaagagt ttagaagttg ttgaaagaat
     6421 cacaggagtt gagattcctt tctatgaggc agatattcgt gacacagata ccctcagaga
     6481 tattttcaag caggaagaac cgactggtgt cattcacttt gctggtttga aggctgttgg
     6541 cgaatcaaca cgtatccctc ttgcctacta tgacaacaat atcgctggaa ctgtcagcct
     6601 tttgaaagcc atggaagaaa acaactgtaa aaacatcatc ttcagttctt ctgcgacagt
     6661 ttacggagat ccgcacacag tgcccatctt ggaagatttc ccactttcag tgacaaaccc
     6721 atacggtcgt accaagctca tgctagagga aattttgacc gatatttaca aagcagactc
     6781 agaatggaat gttgtcttgc ttcgttactt taacccaatc ggagcccatg agagtggtga
     6841 tttgggagaa aatccaaacg gtattccaaa caatctcttg ccatatgtga ctcaagtagc
     6901 cgttggaaaa ttagagcaag tgcaagtgtt tggagacgat tacgatacgg aagatggaac
     6961 aggtgttcgt gactatatcc acgttgtcga tttggctaag ggtcacgttg cagctttgaa
     7021 aaaaatccaa aaaggttcag gactaaacgt ttataacctt ggaactggta aaggttactc
     7081 agttcttgaa attatccaaa acatggaaaa agcggtggga cgtcctattc cttaccgcat
     7141 cgtagaacgt cgcccaggtg atatcgctgc ctgctactca gacccagcaa aagctaaagc
     7201 agaactcggt tgggaagcag aactcgacat cacccaaatg tgtgaagacg catggcgttg
     7261 gcaaagcaag catccaaatg gatttgaaga ctaagatgat gatttcaatc atcgtccctt
     7321 gtttaaacga agaggaagta cttcctcttt tttatcaggc tctggaagct ttacttccag
     7381 atttggaaac agaaatcgag tatgtctttg tcgatgatgg atcaagtgat gggaccttgg
     7441 aactcttaaa ggcctatcgg gagcaaaatc cggcagtcca ttatatttct ttctctcgaa
     7501 attttggcaa agaagcagcc ctttatgcag gcttgcaata tgcgacagga gatttggtgg
     7561 tggtgatgga tgcagacctc caagatcctc ctagtatgtt gtttgagatg aaaaatgtac
     7621 tagacaaaaa tgtagacttg gactgcgttg ggacacggag aactagtcgg gagggagaac
     7681 ccttctttcg cagtttctgt gctgttctct tttatcgcct catgcaaaaa atcagcccag
     7741 tagctctgcc gtcgggtgtc cgtgattttc gtatgatgag aaggtctgta gtcgatgcca
     7801 ttttaagctt gaccgagtcc aatcgttttt caaaggggct cttcgcctgg gtgggtttca
     7861 aaacctacta tttggactat ccaaatgtcg aaaggcaggc tggcaagaca agttggagtt
     7921 ttaggcaact ctttttctac tccattgaag ggattattaa tttttcagat tttcccttga
     7981 ttatagcctt tgtagctggt ctcctatctt gttttctttc tctgctgatg accttttttg
     8041 ttgtggttcg gaccctcatt ttgggcaatc cgacatctgg ttggacctct ctgatggctg
     8101 ttattctctt tcttggaggc attcaactct tgaccattgg gattctcggc aagtatatca
     8161 gtaagattta tttagagact aaaaaaagac cactttatct tatcaaagaa aaaagtgacc
     8221 ttcctgattt tacagaaaaa aataaagtga aaagactata attttacatg gaaatgtgct
     8281 aaactagagg gagtggatta tcgcccttga tttaggcttc ctggtttcca atcagggcgc
     8341 gagttgggca atttttaaca gcctctaaga catcctgact aggagaaatt tctttttcca
     8401 gttggtcagg gtcatcgtaa aaacgcacga ttccattatc gtggtaatca aataaatcag
     8461 aataagtttg gcaaagccca caggcgatac atcgttcagg tataagtgtg attttcatgt
     8521 tcatatttat attgtaataa gaaaagtaaa aaaaaacaag gagtaaggta tggcaaaaga
     8581 accgtggcaa gaagatatct atgatcaaga agaatcaaga gcagagcgtc ggcatcgaaa
     8641 ccacggaggg gctgatagga tggctaatcg tattttgacg atcctagcta gtattttctt
     8701 tgtaattgtg gtggtgatgg tcatcgttct catctatcta tcatcggggg gaagtaatcg
     8761 cacagcagcc ttaaaagact ttcatgattc tgatgcaagt gtagtacaaa tctcatcttc
     8821 aagcagttct catcctgagc agagttcaga gccagaatct acttctagta gttcagaaga
     8881 agctgctaat cctgaaggaa cgattaaagt tctcgcagga gaaggggaag cagctattgc
     8941 cgctcgtgca ggaatctcca ttgctcagtt agaggccttg aatcctgggc acatggctac
     9001 aggatcttgg tttgctaatc caggtgatgt tataaaaata aaataggagc cgatatgaaa
     9061 acaattcaaa ttgctattga tggtcctgct tccagcggta agagtacggt cgcaaagatt
     9121 attgctaagg attttggatt cacctacctt gatacaggag ctatgtatcg tgcagcgacc
     9181 tatatggctc ttaagaacca attaggagtt gaagaagtcg aagcccttct agccttgttg
     9241 gaccagcatc caatcagctt tggacgttca gaaactggag accagcttgt ttttgtagga
     9301 gatgtggata ttacccatcc tatccgtgaa aatgaagtga ccaatcatgt ttctgctatt
     9361 gcagcaattc ctgaagtgcg tgagaaactg gtttctctcc aacaagaaat tgcccagcaa
     9421 ggcgggattg tcatggatgg tcgcgatatt ggaactgttg tattgccaca agcagaattg
     9481 aaaattttcc tagtagcttc tgttgatgag agagcagagc gtcgttacaa ggaaaatatt
     9541 gccaagggaa ttgaaacaga ccttgaaacc ctaaaaaagg aaattgctgc gcgtgactac
     9601 aaggatagtc atcgtgagac ttctcctctc aaacaagcag aggatgctgt ctaccttgat
     9661 acaactggtt tgaacattca agaagtagtt gaaaaaatca aagcagaagc tgaaaaaaga
     9721 atgtaaatgt agaaaagccg atagattgat atcggttttt ttctaaattt gtcaaaatgc
     9781 tgattttagg gtataatagt tgctgttcga gaaattttga ttttcaaaga atagtgaatg
     9841 atgataagga gaaaccatga acaatttacc aaattgtcca aaatgtaact cagagtatgt
     9901 ctacgaagac ggtgccctac tggtttgccc agagtgtgct catgagtgga atcctgctga
     9961 agttgcagaa gtagaagaag gtcttgtcgc tatcgatgcc aacggaaata aattgactga
    10021 tggtgataca gtaactctca tcaaggactt gaaagtaaaa ggtgcgccaa aagatttgaa
    10081 acaagggacg cgcgtgaaaa atatccgcat cgtagaaggc gatcacaata tcgactgtaa
    10141 gattgatggt tttggtgcca tgaaacttaa atcagagttt gtgaggaaga tttaagcatg
    10201 tcaaaacact ataaacttgt attttatagc cgtatcttct tgtttctagc ggcttttacg
    10261 ggagtttatc ttgaaattac taagtatggt ggttttggga tgcttctcta ttacacggtt
    10321 ctgtccaacc tcttggtaac tatttttacc ctttatcttc taaaggttat gagccgtgta
    10381 ggtgaaaatt ggcaaaggcc aagtctcttg cgcttaaaag gtggggtcac catgagtatc
    10441 atgattacct gtgtgattta ccatttcctc ttggcgccca ttgcgactaa tttctatacc
    10501 ctagaaaatt tcctttgcca ctatatcgtt cccatctggt ttttagcgga taccctcttt
    10561 tttgacaaac agggtcaata caagatttgg gatccagcag tgtggaccat tttacccttt
    10621 ctgtatatga tgtttgctct ttttaatggc ttggttctaa aactcaatat tccaaatgcc
    10681 aaggataatc ctttccctta cttctttttg aatgtgaaca agggttggaa tgttgtgttt
    10741 aagtggtgtc tgattatctt tgttgcctat atggtagcag gatttatttt ctactttatc
    10801 aagcaaatca agagaaagtc atcctaatac tcttcgaaaa tctcttcaaa ccacgttagt
    10861 ttccatctgc aacctcaaaa cactgttttg aacaacctgc ggctagcttt ctagtttgct
    10921 ctttgatttt cattgagtat gagatacaag ggattcattt tcgaatttaa gaaggagtct
    10981 acaaaccaat gaggcttctt cttttcttgc ttatttgata actatcaaaa aagtagaaga
    11041 ttaagagaaa tatagaaaga gatttcatga aacaattttt agaacgggcc agcattttgg
    11101 ctctctccct cgttttgatt acctcctttt ccatttcgag tgccctacca gccatgtttg
    11161 actattatca gggttattct aaggaacaaa ttgagctctt ggtgagcttg ccttcctttg
    11221 gaatcatgat gatgttactg ctaaatggtt tcttagaaaa aatatttcct gagcgcttac
    11281 agattagttt gggcttgctg attttatcat tgagcggtac agctcccttc tggtaccaag
    11341 cctatccctt tgtctttgga acacggcttc tctttggttt gggtcttggg atgatcaatg
    11401 ccaaggccat ttctattatc agtgaacgct accaaggaaa aaggcgaatt cagatgttag
    11461 ggctacgcgc ttctgcagag gtcgttggag cttctctcat taccttggcc gtcggtcagt
    11521 tgttggcctt tggttggaca gctatctttc tagcctatag tgctggattt ttggtgatgc
    11581 ccctttatct gctctttgtc ccttatggaa aatcaaagaa agaagtcaag aaaagagcga
    11641 aggaagcaag tcgtttaact cgagaaatga aaggcttgat ttttacctta gctatcgaag
    11701 cggcagttgt agtttgtacc aatacagcta ttaccatccg tattccaagt ttgatggtgg
    11761 aaagaggatt gggggatgcc cagttatcta gttttgttct tagtatcatg cagttgatcg
    11821 ggattgtggc tggggtgagt ttttctttct tgatttctat ctttaaagag aaactgctcc
    11881 tctggtctgg tattaccttt ggcttggggc aaatcgtgat tgccttgtct tcatccttgt
    11941 gggtggtagt agcaggaagt gttctggctg gatttgccta tagtgtagtc ttgacgacgg
    12001 tctttcaact tgtctctgaa cgaattccag ctaaactcct caatcaagca acttcatttg
    12061 ctgtattagg ctgtagtttc ggagccttta cgaccccatt tgttctaggt gcaattggct
    12121 tactaactca caatgggatg ttggtcttta gtatcttagg aggttggttg attgtaatct
    12181 ctatctttgt catgtaccta cttcagaaga gagcttagga ttgattccta agtttttctt
    12241 ttgatactca atgaaaatca aagagcatag ttgattgagt ttggaatagt acgctgtagc
    12301 tgctaaaata tttctagaaa ttaatttgac ttttctaata gagttattca tatcttattt
    12361 caatctacta tagaaagcta gccgcaggtt gctcaaaata gtgtgttgag gttgcagatg
    12421 gaaactgacg tggtttgaag agattttcga agagtatgag atttttatac ccatacaata
    12481 tttgttttag gtccttgttt acagactaat ttcttgataa aatagaggtt atgacgagat
    12541 ataaagcaac tatttcctat gatggttatg cctttgctgg ctttcagcgc cagtctcatg
    12601 cgcgtagcgt tcaggaagaa attgaaaaaa ccttgaccag attaaataaa gggcaaacca
    12661 ttactgttca cggtgctggt aggacagata gtggggttca tgccctggga caggtcattc
    12721 attttgacct gccttatcag atggatgagg agaaactccg ttttgccttg gatacccagt
    12781 ctcctgaaga tattgatgtg atttcgattg agcttgtggc agatgatttt cattgccgtt
    12841 atgccaaaca tagcaagacc tatgagttta ttgtggatag aggacgtccc aaaaatccta
    12901 tgcgccgtca ctatgccacc cactttccct acccactcga tgtggaacga atgcagattg
    12961 caatcaaaaa gctagaggga acccatgatt ttaccggttt tacagcctct gggactagtg
    13021 tagaggataa ggttcgcacc atcacagaag ctagtttaat agtcgatgag acaggacaat
    13081 ttttgacctt taccttttca ggaaatggtt tcttgtataa acagattcgc aatatggtgg
    13141 ggactcttct caaaatcggt aacaaccgca tgccagtaga gcagattgat ctcatcttgg
    13201 agaagaagga caggcaactt gcaggtccca ctgcagcacc aaatggtttg tatttaaagg
    13261 agattcgtta tgaagaataa tcgtatttta gcactttctg gaaatgatat ttttagtggt
    13321 ggtggactgt cagctgattt ggctacctat accttgaacg gcttgcatgg gtttgtagca
    13381 gtgacttgtt tgacagcctt gacagaaaaa ggatttgaag tctttccaac tgatgatacc
    13441 atttttcaac atgaattaga tagcttacgt gatgtggaat ttgggggaat taagattggt
    13501 cttctcccta ctgtcagtgt ggctgagaag gccttggact ttatcaaaca acgcccagga
    13561 gtacctgtgg tgttggatcc tgtcttggtc tgcaaggaaa cgcatgatgt agctgtcagt
    13621 gagctctgcc aagagttgat tcgctttttc ccttatgtca gtgtgattac gcctaatctc
    13681 ccagaagcag aattattatc cggtcaggaa attaaaacct tggaagacat gaaaactgca
    13741 gcgcagaaat tgcatgattt aggagcgcca gcagtcatta tcaagggagg caatcgtctt
    13801 agtcaggaca aggctgtgga tgtcttttat gatggacaga cctttactat cctagaaaat
    13861 ccagttatcc aaggccaaaa tgctggtgca ggttgtacct ttgcctctag cattgccagt
    13921 cacttgatta aaggtgataa acttttgcca gcagtagaaa gctctaaggc tttcgtttat
    13981 cgtgctattg cacaagcaga tcagtatgga gtaagacaat atgaagcaaa caaaaacaac
    14041 taaaatcgcc cttgtatccc tattaaccgc cctttctgtg gttctaggtt atttcttaaa
    14101 aatcccaaca cctacaggaa ttctaactct tttagatgct ggtgtcttct ttgcggcctt
    14161 ttactttggt agtcgtgaag gagcggtagt cggaggacta gcaagtttct tgattgacct
    14221 cttatcaggc taccctcagt ggatgttctt tagcttggtc aaccatggct tgcagggatt
    14281 tttcgcagga tttaaaggaa aaagtcagtg gttaggcctt attttagcaa ctattgccat
    14341 ggtaggaggc tacgccttgg gttctacttt gatgaatggc tgggcagcag ccctcccaga
    14401 aattctacca aatttcatgc aaaatatggt agggatgatt gtaggattta ttcttagtca
    14461 aagtatcaag aagattaagt aatactcttc gaaaatctct tcaaaccacg tcagcttcca
    14521 tctgcaacct caaaacactg ttttgagcag cctgaggcta gctttctagt ttgcactttg
    14581 attttcattg agtataaaga ggttgagtaa aaagtatttc aaataagaaa aacgcatagt
    14641 atcaggtgtt gaatgaacta cccccc
//
LOCUS       DAVMGZ010000038        14478 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000038 DAVMGZ010000000
VERSION     DAVMGZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.039"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            481..2247
                     /locus_tag="VYQ93_000967"
     CDS             481..2247
                     /locus_tag="VYQ93_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HEV3335676.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTVVRAEEAPVAKQSQAER
                     DYDAAMKKSEAAKKEYEEAKKDLEEAKAAQKKYEDDQKKTEEKAKLVQKADEERQKAN
                     VAVQKAYLKLREAQEQLNKSPNNKKNAAQQKLKDALAHIDEVTLKQKEAEANFNKEQA
                     KVVPEADKLAETKKKAEQAEKKEPELAKKVAEAKAKAEEAEKKAVEAKQKVDAEKYAL
                     EAKIAELEYEVQGLEKELKEIDESDSEDYIKEGLRAPLQSKLDAKKAKLSKLEELSDK
                     IDELDAEIAKLEKDVEDFKNSDGEQAEQYLVAAKKDLDAKKAELENTEADLKKAVDEP
                     ETPAPAPAPKPAPAPAPTPEAPAPAPKPAPAPKPAPAPKPAPAPKPAPAPKPETPKTG
                     WKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGAMATGWLEYNGSWYYLNSNGAMA
                     TGWLQYNGSWYYLNANGAMATGWLEYNGSWYYLNSNGAMATGWLQYNGSWYYLNANGA
                     MATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKANQWFKVSDKWYYVNG
                     LGALAVNTTVDGYTVNENGEWV"
     gene            2647..3768
                     /gene="mnmA"
                     /locus_tag="VYQ93_000968"
     CDS             2647..3768
                     /gene="mnmA"
                     /locus_tag="VYQ93_000968"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV3335677.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTTLLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVVRDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLKKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTRDMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            3909..4364
                     /locus_tag="VYQ93_000969"
     CDS             3909..4364
                     /locus_tag="VYQ93_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3335678.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNRKLLVTKDKGKYYTIGGA
                     IQVNEKTEDAVVREVKEELGVKAQAGQLAFVVENRFEQDGFSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKFEDIQLVPAFLKTALPDWEGQLRHIHREE"
     gene            4374..6287
                     /gene="mnmG"
                     /locus_tag="VYQ93_000970"
     CDS             4374..6287
                     /gene="mnmG"
                     /locus_tag="VYQ93_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV3335679.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDKILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDAVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEELDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            6476..6565
                     /locus_tag="VYQ93_000971"
                     /pseudo
     CDS             6476..6565
                     /locus_tag="VYQ93_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(6666..8345)
                     /locus_tag="VYQ93_000972"
     CDS             complement(6666..8345)
                     /locus_tag="VYQ93_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV3335680.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQGEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8347..8580)
                     /locus_tag="VYQ93_000973"
     CDS             complement(8347..8580)
                     /locus_tag="VYQ93_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV3335681.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            8962..9183
                     /locus_tag="VYQ93_000974"
     CDS             8962..9183
                     /locus_tag="VYQ93_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335682.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(9396..9548)
                     /gene="cibB"
                     /locus_tag="VYQ93_000975"
     CDS             complement(9396..9548)
                     /gene="cibB"
                     /locus_tag="VYQ93_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV3335683.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(9550..9735)
                     /gene="cibA"
                     /locus_tag="VYQ93_000976"
     CDS             complement(9550..9735)
                     /gene="cibA"
                     /locus_tag="VYQ93_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV3335684.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            9913..10596
                     /gene="tsaB"
                     /locus_tag="VYQ93_000977"
     CDS             9913..10596
                     /gene="tsaB"
                     /locus_tag="VYQ93_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV3335685.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVSLMDARRNNVYVGFYENAKPVMPEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQEYLPRTNFKETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            10593..11030
                     /gene="rimI"
                     /locus_tag="VYQ93_000978"
     CDS             10593..11030
                     /gene="rimI"
                     /locus_tag="VYQ93_000978"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV3335686.1"
                     /translation="MIEIKRIQKHSDLAQAIYAVMIAVYPVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            11020..12030
                     /gene="tsaD"
                     /locus_tag="VYQ93_000979"
     CDS             11020..12030
                     /gene="tsaD"
                     /locus_tag="VYQ93_000979"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV3335687.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(12070..13376)
                     /locus_tag="VYQ93_000980"
                     /pseudo
     CDS             complement(12070..13376)
                     /locus_tag="VYQ93_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(13709..14003)
                     /locus_tag="VYQ93_000981"
                     /pseudo
     CDS             complement(13709..14003)
                     /locus_tag="VYQ93_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14110..14403)
                     /locus_tag="VYQ93_000982"
                     /pseudo
     CDS             complement(14110..14403)
                     /locus_tag="VYQ93_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         4307 a         2832 c         3353 g         3986 t
ORIGIN      
        1 gctagggatt tatctaaaaa tactatgaaa ttggacgtca tattttttga ggataaaaat
       61 aaaagatccg cagttttagg tttacgaaga gacaaaagcg gagtatttaa atcagttact
      121 ctacatttta caagcgctaa gaaatatgct aaagttcgta aaacagatgt gaaagaaatg
      181 aaatggctat aaagattgtc cgcaggctta agcttgcgtt ttttgttgtt taacgaaatt
      241 aaaaaacaag ctctctcatc ggaaatgttt ttataggaat gaaggaagat gatgctatgt
      301 ttaaaaagag aatcgaaaag ggcaaaagta gtatctttat ctattttttg gagtagaagc
      361 ttatgatata gaaatttgta ataaaaatgt aatataaaac atttgacaaa tatttacggg
      421 gggagtatac ttaatataag tatagtctaa aaatgactat cagaaaagag gtaaatttag
      481 atgaataaga aaaaaatgat tttaacaagt ctagccagcg tcgctatctt aggggctggt
      541 tttgttgcgt ctcagcctac tgttgtaaga gcagaagaag ctcccgtagc taagcagtct
      601 caagctgaga gagactatga tgcagcaatg aaaaaatctg aagctgctaa gaaagaatac
      661 gaagaagcta agaaagattt agaagaagca aaagctgctc agaaaaaata tgaagacgat
      721 caaaagaaaa ctgaagagaa agcaaagctt gttcaaaaag cggatgaaga gcgtcaaaaa
      781 gcgaatgtgg cagttcaaaa ggcgtattta aaactccgag aagcacaaga acaattgaac
      841 aaaagtccaa acaataagaa aaatgccgct caacagaaat taaaagatgc attagctcat
      901 attgatgagg taacattaaa acaaaaagag gcggaagcga atttcaataa ggaacaagca
      961 aaagtagttc ctgaagcaga taagttagct gagactaaga aaaaagcaga acaggctgaa
     1021 aaaaaagaac cagaacttgc taaaaaagta gcagaagcta aagcaaaagc agaagaagct
     1081 gagaaaaaag ctgttgaagc caaacaaaaa gtggatgctg aaaaatatgc tcttgaagct
     1141 aaaatcgctg agttggaata tgaagttcag ggactagaaa aagagctcaa agagattgat
     1201 gagtctgact cagaagatta tattaaagaa ggtctccgtg ctcctcttca atctaaattg
     1261 gatgccaaaa aagctaaact atcaaaactt gaagagttga gtgataagat tgatgagtta
     1321 gacgctgaaa ttgcaaaact tgaaaaagat gtagaagatt tcaaaaactc agacggtgag
     1381 caagctgaac aatatcttgt tgcagctaaa aaagacttag atgctaaaaa agctgaatta
     1441 gaaaatactg aagctgacct taagaaagca gttgatgagc cagaaactcc agctccggct
     1501 ccggctccta aaccagcccc agctccagct ccaactccag aagccccagc tccagctcct
     1561 aaaccagctc cggctcctaa accagcccca gctcctaaac cagctccggc tcctaaacca
     1621 gctccagctc ctaaaccaga aactccaaaa acaggctgga aacaagaaaa tggtatgtgg
     1681 tacttctaca atacggatgg ttcaatggca acaggctggc tacaaaataa tggctcatgg
     1741 tactacctca acgctaatgg tgctatggcg acaggttggc tagaatacaa tggctcatgg
     1801 tactacctca acagcaatgg cgctatggcg acaggttggc tccaatacaa tggttcatgg
     1861 tactacctca acgctaatgg tgctatggcg acaggttggc tagaatacaa tggctcatgg
     1921 tactacctca acagcaatgg cgctatggcg acaggttggc tccaatacaa tggttcatgg
     1981 tactatctaa acgctaacgg cgctatggca acaggttggg ctaaagtcaa cggttcatgg
     2041 tactacctca acgctaacgg ttcaatggca acaggttggg tgaaagatgg agatacctgg
     2101 tactatcttg aagcatcagg tgctatgaaa gcaaaccaat ggttcaaagt atcagataaa
     2161 tggtactatg tcaatggctt aggtgccctt gcagtcaaca caactgtaga cggatatact
     2221 gtcaatgaaa acggtgaatg ggtttaagcc gattaaatta aatcatgtta agaacatttg
     2281 acattttaat tttgaaacaa agataaggtt cgattgaata gatttatgtt cgtattcttt
     2341 aggtacctgt cttatgattt caggaaatgt cattaaaaaa acgactcctt ttctctaact
     2401 tggaaaatag attagagaaa atgggttgtt ttatttatta tagttatttg aatgaagata
     2461 agaagaaggt atactcacat cattcacaaa tctgtatatt gactataagt tttaaaaaac
     2521 aatttttaag ctcttccttg tcttctctaa ccaagcgtgt tataatgaat actgctcaag
     2581 cgaccttcaa tcgtgaagca cacacgacct tcaatcgtga ataaacgaat agatgggaga
     2641 cttaccatga gtgataactc taaaacacgt gttgtcgtgg ggatgagtgg tggtgttgat
     2701 tcgtcggtga cgactcttct tctcaaggag cagggctacg atgtgatcgg tatcttcatg
     2761 aagaactggg atgacacaga tgaaaacggc gtctgtacgg cgaccgaaga ttacaaggat
     2821 gtggttgcgg tggcagacca gattggcatt ccctactact ctgtcaattt tgaaaaagag
     2881 tactgggacc gcgtttttga gtatttccta gcggaatacc gtgcgggacg cacgccaaat
     2941 ccggacgtta tgtgcaacaa ggaaatcaag ttcaaggcct ttttggacta tgctatgacc
     3001 ttgggggcag actatgtagc gactgggcat tatgctcgag tggtgcgtga tgaggatggc
     3061 actgttcaca tgcttcgtgg cgtggacaat ggcaaggacc agacctattt cctcagccaa
     3121 ctttcgcaag aacaacttca aaaaaccatg ttcccactag gacatttgaa aaagcctgaa
     3181 gttcgaaaac tagcagaaga agcaggtctt tcgactgcta agaagaaaga ctcgacaggg
     3241 atttgcttta tcggagaaaa gaactttaaa aacttcctca gcaactacct gccagctcag
     3301 cctggtcgca tgatgactgt ggatggtcgc gatatgggcg agcatgcagg tcttatgtac
     3361 tatacgattg gtcagcgtgg cgggcttggt atcggtggac aacacggtgg tgacaacgcg
     3421 ccgtggttcg ttgtcggaaa agacctgagc aagaatattc tctatgtagg ccaaggtttc
     3481 taccatgatt cgctcatgtc aactagccta gaggctagcc aagtccactt tactcgtgat
     3541 atgcccgagg agtttacgct agaatgtacg gctaaattcc gttaccgtca gcctgactct
     3601 aaggtgaccg tccatgtcaa aggagacaag gcagaagtta tctttgcgga accacaacgc
     3661 gcgattacac caggacaggc agttgtcttt tacgatggcg aagagtgtct aggtggcggt
     3721 ttgattgaca atgcttaccg cgatggacaa gtttgtcagt acatttagat tgacaaattt
     3781 tctcaatttg ctacaataat aacagcaata gaaatgatgg tcaaagctca tggatgttgc
     3841 aggctttttt gtcctgcact tctttggagt tttgactgtt tttgtgtcgt ttaagggaaa
     3901 ggacaagaat gactcaacaa gactttcgga caaaagtaga caatacggtt tttggtgttc
     3961 gggcgacagc tttgattctt caaaatcgca agcttctagt caccaaagac aagggcaagt
     4021 attacactat cggcggtgcg attcaagtta acgaaaaaac ggaagacgcg gtagtccgtg
     4081 aagtgaagga agaactgggt gtcaaagctc aagctgggca gctagctttt gtggttgaaa
     4141 atcgttttga acaggacggt ttttcctatc acaatatcga atttcattat ctggtggact
     4201 tgctggagga tgccccattg accatgcagg aagatgagaa aaggcagccc tgtgagtgga
     4261 ttgacttgga taagtttgag gatatccagc tagttccagc ctttttaaaa acggccctac
     4321 cagattggga aggccaacta agacacattc atcgtgagga ataggagaga aacatgactt
     4381 atcattttac tgaagaatac gatattattg taattggtgc gggacacgct ggggttgagg
     4441 cttccttggc cgctagccgt atgggctgta aggttctact tgcgaccatc aatattgaaa
     4501 tgctggcttt catgccttgt aatccctcta tcggtggttc tgctaagggg attgtcgtac
     4561 gtgaagtcga tgccctcggt ggcgagatgg ccaagaccat tgacaagact tacatccaga
     4621 tgaagatgct caacacaggg aagggtcctg ctgttcgtgc ccttcgtgcg caggctgaca
     4681 aggaacttta ctctaaggag atgcgcaaga cagttgaaaa tcaagaaaat ctgacccttc
     4741 gtcaaaccat gattgataag attttggtgg aagatggcaa ggttgtcggt gttcgtacag
     4801 cgactcatca agagtatgct gctaaggctg ttattgtgac gacagggact gctctccgtg
     4861 gggaaattat catcggagac ctcaagtact catcaggtcc taaccacagc ttggcttcta
     4921 ttaacctagc tgacaatctc aaggaactgg gtctcgaaat cggtcgtttc aagacaggaa
     4981 cccctccacg tgtcaaggcc tcttctatca actacgacgt gacagaaatc caaccaggag
     5041 acgcagtacc taatcatttc tcatacactt cacgtgatga ggattatgtc aaagatcaag
     5101 tgccatgctg gttgacctat accaatggta ccagtcatga gattattcaa aacaacctcc
     5161 accgtgcgcc tatgtttaca ggtgtggtca agggagtggg gcctcgttac tgtccgtcga
     5221 ttgaagacaa gattgtgcgc tttgcggaca aggaacgtca ccaactcttc cttgagccag
     5281 aagggcgcaa tactgaggaa gtctatgttc aaggactttc aaccagtctg cctgaggatg
     5341 tccagcgtga cttggttcat tccatcaaag gtttggaaaa tgcagagatg atgcggacag
     5401 gttatgctat tgagtatgat atggtcttgc ctcatcagtt gcgtgcgact ttggaaacca
     5461 agaaaatctc aggtctcttc actgctggtc agacaaatgg aacgtcaggt tacgaagagg
     5521 cagcaggcca agggattatc gcaggtatca atgcggctct aaaaatccaa ggcaagcctg
     5581 aattgattct aaaacgaagt gacggttata tcggggtgat gatcgacgac ttggtgacca
     5641 agggaaccat tgaaccttac cgtctcttga ccagtcgtgc tgaatatcgt ctcattcttc
     5701 gccatgacaa tgctgatatg cgcttgactg agatgggccg tgagattggc cttgtggacg
     5761 atgaacgctg ggctcgtttt gaaatcaaga aaaatcaatt tgataatgag atgaaacgcc
     5821 tagacagtat caaactcaag ccagtcaagg aaaccaatgc taaggttgag gaaatgggct
     5881 tcaagccgtt gacagatgcg gtgacagcca aggaattcct tcgccgtcca gaagtttctt
     5941 accaagatgt ggtggccttc atcggaccag ctgcagagga attagatgac aagattatcg
     6001 aattgattga aacagaaatc aagtacgaag gctatatttc caaagccatg gatcaggttg
     6061 ccaagatgaa acgtatggaa gaaaaacgca ttccagccaa tatcgactgg gatgacatcg
     6121 attctattgc gacggaagct cgtcagaagt tcaaactcat caatccagaa accatcggcc
     6181 aagccagccg tatttcggga gtaaacccag cagatatttc tattttgatg gtgtatctgg
     6241 aaggtaaaaa tcgtagtatt tctaaaactc ttcaaaaatc aaaatgatac gtcgtaggct
     6301 tcttacgaat gagttcaaag cttggctttg attcatctcc aacctcccat agttccccga
     6361 actatgggag ctaactcaag ttatgcattt tcctagcctt ctggtctgat tttcattgag
     6421 ttttaatcta aaaagaaagc atagagaaaa acagctccga agacggggct gttttgttga
     6481 tttgtactca atgaaaatca aagagcaaac taggaagcta gccgtaggtt gctcaaagca
     6541 ctgctttgag gttgtggata gaactgacga agtcagctca aaacaccgtt ttgaggttgt
     6601 ggatagaact gacgaagtca gtaacatata tacggcaagg cgaagctgac gtggtttgaa
     6661 gagtattatt cttcatctgg tgtgaggatc atcgggatga tgatcggttc acgttcggta
     6721 ttttcataga ggaaggggcg aatagcgttg acaatggcac cattgacaga ttgcacgcta
     6781 gcatccttat ttttcagtgc gatacgaatg gcattgaaga ggatacgctg gctttgacga
     6841 atcaagtcgc cagactctct catgtagaca aagcctcggc tgaggatgtc tggaccagat
     6901 agaatcatct gcgatttgaa gtcaacagtt gcgactgcca gaacgacacc gtcttcagat
     6961 agatcgcgac gatctttgag gacagctgcg ccaatttcac cgatacgatt tccatcgaca
     7021 tagatatctt gggcgttgaa atggcctgcg atacgagctg agtcagcagt aagggcaagc
     7081 acatcgccat tgctcatgat aaagatattg tccttctcaa caccagtatc cactgctagt
     7141 ccagcgtgga ctttttgcat gcggtattca ccgtggacag gcatgaagta ttttggctta
     7201 atcaagcaga gcatgagttt ttgctcttgc tgaccaccgt gtccagatgt atggatattg
     7261 ttcactttac cgtggataac ttcgacacca gcttcagaaa tgatgttaat cagcttgttg
     7321 acgctagtag tgtttccagg gatgggacta gaagagaaga taacggtatc acctggttgt
     7381 aattgtactt gacggtgggt tccgttggcg atacgagaga gggctgccat aggctcaccc
     7441 tgactacctg tacagaggat aagaacttct cctgcaggat aatctttgat ttcatttggc
     7501 tcgataaagg ttcccttagg agctttgatg tagccaagat cgattccgtt gacaatggcc
     7561 ttttccatag aacgaccaaa gaccgcaatc ttgcgtccag tcttaacagc agcttctgtt
     7621 gcctgctgga gacggaagat atttgaggca aaggatgcaa agatgatacg tccttcaata
     7681 ccttggataa tcttcataat ggactgacca acgacttttt cagagttggt aaaggttggt
     7741 acttccgcat ttgtcgagtc agacaggaga cagagcacgc cttcttcacc aagcgcagcc
     7801 atacgatgca agtccgcagg ttctccaact ggagtaaagt cgaacttaaa gtcacccgta
     7861 cagacgattt tcccttgagg agtatgaatg acaatcccca aaggctctgg aatagagtga
     7921 gtcgttctaa agaaagttgc cttgagattt ttaaaggtca actcggtgtt gtggttgatt
     7981 tcgtaaagtt tggcgttgcg caagaggccg tgttcttcga gtttcccacg gatcaaagcc
     8041 aaggcaagcg gtccagcata aatagggaca tttgcttgct tgagtaggaa cggaatccca
     8101 ccaatgtggt cctcgtgtcc gtgtgtgatt aaaacagcct tgacgcggtc gatattgtct
     8161 acgatgtaag agtagtcagg aatgacatag tcgataccaa gcaagtcatc ttctgggaat
     8221 ttaatcccag catcgacgat gataatctcg ccttggtatt caattccgta agtgtttttc
     8281 ccgatttctc ctagaccacc gatggcaaaa acgccgactt cttcaggttt aagagtgtag
     8341 gccatattag aactccgtaa tttcgaaggc gccagtttct ttttcgtaat cgagcaattt
     8401 gtcagacaag agttcgatat attcgatatt gtactctggg cgattttctt cgacaagttg
     8461 gcgagcagtg atacggccct caagttctga gctggcatcg atgtctaggt aaagcgcgcg
     8521 tgttgtttca cggcgtgggc tacgttcttt tgtttcttga taaaaaactt tgtaaatcat
     8581 atagttcctt tctatttaca ggtcagctta ttccaaactt ttagcagata tttgcttgat
     8641 ttcattccat tttgtgtcta gattgacctt gattcgttac aattatttca attataccac
     8701 aaaatgaaaa attagtaaaa gacggaaacg agaaagttag gggacgattt cttcggtaag
     8761 cgtttgcgaa aataaagaaa atgtattatg ttgataacgg aagtgaaatt tattgcttgt
     8821 cataattcca tatagatttt aagtcaagga ggttgcccta tgtataactt atttgctttt
     8881 gctatcggag ttcgtttgct agcttttatc gtttttgttt tgcgtttatg gaggaaatct
     8941 tctatttgcc aaactggaac gatgacaaac caagttcttg aggaaaatta cctttgtgac
     9001 tctctactcg ctttttctcc tcctcgtgac ctatgtcgtt tggtttgtat tttactttgg
     9061 attaaatgct tagatgccct gcttatgctg gggttttttg tttggggatg tagagatagt
     9121 agatttacga cacgaaaatt ccaccccatc tatcaagatg aggtggtctt tgcatttttc
     9181 taaaatgata tgacaatgga attttcaaag cttattattt ttatgggagt cattgttctg
     9241 ctcaaaaata gagttgaagc ataaaaataa aacaatacaa acagataaat cgaatggtaa
     9301 agggatggta gtaatgttca aatcattgag aatgctgagc aaactgtaag agatgaagac
     9361 aataaaattt atccttctaa aaatgtgttt cttatctagc atgacttacc aaacttttta
     9421 cgaaggtctt ttccaatggt ataacctgcc ataaataaag cgataccaac acctacacca
     9481 aagccaccct tgatttcttg caattcctta ttagaaattt cacgacagtt gttcaaatct
     9541 ttcatcatat taatctcctc ctaaaaaata accagctgcc aaaccagaac ctataagtgc
     9601 tgttccacct gcaatagctt tccaagatac tgctactcca aagataacca agggagcgag
     9661 accgccatca atatctgata attcattttc agataaggat aaaaattgat tgtcaagaat
     9721 gtcaaaattt gtcattgaac tttcctcctt ttttcttgtt cacttttata ttcggaaaag
     9781 aaaaggaaaa gtgagcattt ttttaaatta tttttagtta agtgagggca ctcttgtctg
     9841 ggggcaaaga aaattcccat gtgccagctt ttgtagtata atagtaagca gacaaaaaga
     9901 taggaatgtg ttatgaaagt attagctttt gatacgtcca gcaaggctct ttctctcgct
     9961 attttagagg acaagcaggt tcttgccgag acgacgatta atattaagaa aaatcacagt
    10021 attactctta tgcctgccat agattttttg atggcaagtt tggattggac acccaaggat
    10081 ttggaccgaa tcgtggtagc tgaagggccg ggtagctaca caggcttgcg aattgcggta
    10141 gccactgcca agactttggc tcatactctg aacatcgagt tagttggtat gtctagcctc
    10201 ttggctctgg taccctacca acaagaaggc ttgttcgtct ctttgatgga tgcgcgtcgc
    10261 aacaatgttt atgtaggatt ttatgaaaat gctaaacccg tcatgccaga agctcactta
    10321 ccctttgagc gagtgattga gttaatcaag ggtgctagtc aggttacctt tgtcggagaa
    10381 gttggcccct ttgttgagca gattcaagaa tacttgccaa ggactaattt caaagaaacc
    10441 ttgccaaatg cagctaatct agctctatgg gcctgggaca aggaagcaga ctccttgcat
    10501 gattttgtgc cgaactacct caagcgtgtc gaagctgagg aaaactggct caagaatcac
    10561 accgagtctg gcgagtctta cattaaacgc ttatgataga aatcaaacga atccaaaaac
    10621 attctgactt ggctcaagcc atctacgctg ttatgatagc tgtttaccca gtcagtcctt
    10681 ggacgctgga acaaatccaa gcagacctgt cccaagacca aacttggtat gctttggctt
    10741 atgatggggc agaagtgatt ggatttctag ctgtgcagga gaatcttttt gaagcagaag
    10801 tcctgcaaat cgctgtcaaa ggagcttatc agggtcaggg aattgcgtca gccttgtttg
    10861 ctcaattgcc gacagacaag gaaattttcc tcgaagtcag acagtcaaat caacgagcgc
    10921 aagcatttta caagaaagaa aagatggcag ttatcgctga gcgaaaggcc tactaccatg
    10981 acccagtcga ggacgccatt atcatgaaga gagaaataga tgaaggatag atatatttta
    11041 gcatttgaga catcctgtga tgagaccagt gtcgccgtct tgaaaaacga cgatgagctc
    11101 ttgtccaatg tcattgctag tcaaattgag agtcacaaac gttttggtgg cgtagtgccc
    11161 gaagtagcca gtcgtcacca tgtcgaggtc attacagcct gtatcgagga ggcattggca
    11221 gaagcaggga ttaccgaaga ggacgtgaca gctgttgcgg ttacctacgg accaggcttg
    11281 gtcggagcct tgctagttgg tttgtcagct gccaaggcct ttgcttgggc tcacggactt
    11341 ccactgattc ctgttaatca catggctggg cacctcatgg cagctcagag tgtggagcct
    11401 ttggagtttc ccttgctagc ccttttagtc agtggtgggc acacagagtt ggtctatgtt
    11461 tctgaggctg gcgattacaa gattgttggg gagacacgag acgatgcagt tggggaggct
    11521 tatgacaagg tcggtcgtgt catgggcttg acctatcctg caggtcgtga gattgacgag
    11581 ctggctcatc aggggcagga tatttatgat ttcccccgtg ccatgattaa ggaagataat
    11641 ctggagttct ccttctcagg tttgaaatct gcctttatca atcttcatca caatgccgag
    11701 caaaagggag aaagcctgtc tacagaagat ttgtgtgctt ccttccaagc agcagttatg
    11761 gacattctca tggcaaaaac caagaaggct ttggagaaat atcctgttaa aaccctagtt
    11821 gtggcaggtg gtgtggcagc caataaaggt ctcagagaac gcctagcagc cgaaatcaca
    11881 gatgtcaagg ttatcatccc ccctctgcga ctctgcggag acaatgcagg tatgattgcc
    11941 tatgccagcg tcagcgagtg gaacaaagaa aacttcgcag gctgggacct caatgccaaa
    12001 ccaagtcttg cctttgatac catggaataa agagtggctc tttgtcaagt gtagtgggta
    12061 ggcgaaaagc tacaatctgg agagtacgaa attcgtactc tcttttttga tgttcaaagt
    12121 catcaaaatc cgttttttaa aattgttaaa gttccgaaaa ccaaaggctt tgcgcttgat
    12181 aaccttaata aaattattgg tcgcttcaag tttagcgttg gaataaggca gcttaagtgc
    12241 gttgataatc ttatctctat cccttaaaaa agtccaaaag accgtttgaa aaagcggatg
    12301 aaccgttggc agttcttgct caatcaatcc aaagaaatgg ttggcattct tctcttgaaa
    12361 atgaaagagc aggagttgat agagttcgta gtgaacctgt aatccctcag agtaagaaag
    12421 cagcttatct cgtacttccc gatgggttaa gtgcattcga aacgtgggct gatagaatcg
    12481 tttctgacag agcttgaagc tatcctggtg tagtaaatca gtccagtgga ctgatttagc
    12541 ccgagcctag aaacgaaaaa gcgagggttc caaaagcgct tgagcgcccg atactccaag
    12601 gattttcggt caaattggtt catgatttgg attcgtactc ggttcatagc tcggctcaga
    12661 tgttggacaa tgtggaggcg gtcaagaaca atcttcgcct ttggaaagag ttgcttagcg
    12721 agccgataat aagggctgta catgtccatg gtgatgacct cgaccagctc ccgaacctct
    12781 ctctgatagc gttggaaatg gtttcgaatc accgcatgag ttcgcccgtc taaaattgcg
    12841 aggatggatt tggactcaaa atcttgggca atgaagctca ttttgctctt tttgaagcta
    12901 tactcatccc aactcataac ttttggcaac ttggtccaat cggtttcaaa cttaaattcc
    12961 ctcagttttc ggatgacggt ggaagttgag accgccaatc tgtgggcaat ctcagtcata
    13021 gactgctttt caaggaggag ctgagcgatt ttttgtctca ccatgttgga aatctggcaa
    13081 tttttcttga caatggatgt ttgagaaacg accaccttaa ggcaattctt gcactgaaag
    13141 cggcgctttt tgagatgcag taccgtgggt aaaacctgac agtcgagaag cggaatttta
    13201 gaggctttct ggaagtcgta tttgatcatc ttcccttgac aatgaggaca aggaggagcg
    13261 ggggaatcca actttgcctg aacgactaga tgggtttggt atttcagaac agacaagatt
    13321 ttgatatttt tgtcttccaa tccgagcaaa tctgtggtat tcttaagttg ttccatatga
    13381 accctttcta atgagttggt tagacgcttt tcattatagg tcatatgggg ctttttttct
    13441 acaagaaacg accctataat tcctggggtg ggattaccca ctacagaaat tatagagcca
    13501 aagcattcca aagtcttgtc tgactctgga atgttttctg ttcacagttg ttttgtaact
    13561 gcctaataag taaccgcgct ataacgaggt atctaccgag attgtcaatt ctccagtata
    13621 ctgttataaa aaacaaaact ggaggatttt tttatgtccc cccatcgtcc catcgactgt
    13681 tccgtagctg cccaataacg gagtataatc agctgaaaca agaataggac aaaagtgcct
    13741 caagaaaagc atcgtaattt gacaatatta ttctgagatg tttttgatgt aagcccatat
    13801 tttctcaatg ggattatact cgggtgaata gataggaagt ggtaacagtc tatgccctgc
    13861 tccttacaca tgttcattct gtgaaacctt gcattgtcca taatgataag ggatggtgta
    13921 tctaaagtag gtagtaagaa tttttggaag caagcttcga aaaagtcact cgtcatcgta
    13981 tctttatagg tcatcggagc catagtatat tgaaacaaaa aagggcatta gtacttctat
    14041 aacagtatcg caatctctgg tgtgcgttgt ctcgatctta attcaaataa ttatatcttc
    14101 ttccagagag actaactgcc tgctcaaggc tcgatcatat tcgagataaa aataggtctt
    14161 aacccctgtc tcataaatat aaataggagt caggtagctt aagtgattca attctttaag
    14221 gaaccgattt actttttctg ggtcttgttc gcagtaggta cggctctttt ttagactata
    14281 tcccatagcc tttagagcgt aatgaatagt tgttggagga cagttgaatt cagcagctat
    14341 ttcagtcaaa taagcatcgg gatgagtttc aatatagatc tttaatttat ctctatcaac
    14401 tttttcaatt aatagtggaa gttttcttaa agttagtgta ttgaaacgtt tttatacagt
    14461 gctaaaattt gaagtgta
//
LOCUS       DAVMGZ010000039        14444 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000039 DAVMGZ010000000
VERSION     DAVMGZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14444
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.040"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            30..572
                     /locus_tag="VYQ93_000983"
     CDS             30..572
                     /locus_tag="VYQ93_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV3335688.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(613..686)
                     /locus_tag="VYQ93_000984"
     tRNA            complement(613..686)
                     /locus_tag="VYQ93_000984"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(650..652),aa:Asn,seq:gtt)
     gene            complement(692..763)
                     /locus_tag="VYQ93_000985"
     tRNA            complement(692..763)
                     /locus_tag="VYQ93_000985"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(728..730),aa:Glu,seq:ttc)
     gene            complement(815..1567)
                     /locus_tag="VYQ93_000986"
     CDS             complement(815..1567)
                     /locus_tag="VYQ93_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3335689.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1564..2889)
                     /locus_tag="VYQ93_000987"
     CDS             complement(1564..2889)
                     /locus_tag="VYQ93_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3335690.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYVFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVMVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNMKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(2910..3035)
                     /gene="comC"
                     /locus_tag="VYQ93_000988"
     CDS             complement(2910..3035)
                     /gene="comC"
                     /locus_tag="VYQ93_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV3335691.1"
                     /translation="MKNTVKLEQFVALKEKDLQNIKGGEMRISRIILDFLFLRKK"
     gene            complement(3200..3273)
                     /locus_tag="VYQ93_000989"
     tRNA            complement(3200..3273)
                     /locus_tag="VYQ93_000989"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3237..3239),aa:Arg,seq:tct)
     gene            complement(3317..3796)
                     /gene="rlmH"
                     /locus_tag="VYQ93_000990"
     CDS             complement(3317..3796)
                     /gene="rlmH"
                     /locus_tag="VYQ93_000990"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV3335692.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            3979..5160
                     /locus_tag="VYQ93_000991"
     CDS             3979..5160
                     /locus_tag="VYQ93_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV3335693.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            5218..5976
                     /locus_tag="VYQ93_000992"
     CDS             5218..5976
                     /locus_tag="VYQ93_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV3335694.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            6189..7550
                     /gene="dnaA"
                     /locus_tag="VYQ93_000993"
     CDS             6189..7550
                     /gene="dnaA"
                     /locus_tag="VYQ93_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV3335695.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            7709..8845
                     /gene="dnaN"
                     /locus_tag="VYQ93_000994"
     CDS             7709..8845
                     /gene="dnaN"
                     /locus_tag="VYQ93_000994"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV3335696.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            8910..9104
                     /locus_tag="VYQ93_000995"
     CDS             8910..9104
                     /locus_tag="VYQ93_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV3335697.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            9188..10303
                     /gene="ychF"
                     /locus_tag="VYQ93_000996"
     CDS             9188..10303
                     /gene="ychF"
                     /locus_tag="VYQ93_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV3335698.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            10375..10944
                     /locus_tag="VYQ93_000997"
     CDS             10375..10944
                     /locus_tag="VYQ93_000997"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV3335699.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSIDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            10945..>14444
                     /gene="mfd"
                     /locus_tag="VYQ93_000998"
     CDS             10945..>14444
                     /gene="mfd"
                     /locus_tag="VYQ93_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV3335700.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIVASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIHDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEE"
BASE COUNT         4968 a         2413 c         2587 g         4476 t
ORIGIN      
        1 atagtctaat ttgtttacaa ggaggaaata tgcaagaaag taacaaacgc ttaaaaacaa
       61 agcgaactat tgaaaacgct atggtacaat tactgatgga acagccattt gataaaattt
      121 ctactgtcaa attagtagaa aaagccggaa ttagtcgttc cagcttctat actcactata
      181 aggataagta tgatatgatt gagcactatc aaagcaagct attccataca tttgaatata
      241 tttttcaaaa acatgctcat cacaaaagag acgctatttt agaagttttt gaatatctag
      301 agtcagaacc acttctggct gcccttcttt ctgaaaatgg gactaaagaa atccaaaatt
      361 tcttacgaaa taaacttcat atcatgctca gtacagattt acaaaagcga tttatgcaac
      421 tgaatctaaa taccactgaa ttagaatata gtagcatcta tctaactcat gcactttttg
      481 gtgtctgcca aacttggatt gcacatggaa aaaaagaaag ttctcaagaa ataacagact
      541 tccttatgaa aatgcttggt gatacaaatt gatacaaaaa gaggaacaca gctgtgttcc
      601 cttttatcta tactccgcca gtaggactcg aacctacgac atcatgatta acagtcatgc
      661 gctactacca actgagctat ggcggataaa atagtccgta cgggattcga acccgtgtta
      721 ccgccgtgaa aaggcggtgt cttaacccct tgaccaacgg accttctatc tgtagcagat
      781 ataaccatta tatcaatttc ttgctaattg tcaatcactt ttgagatttt ttctctaaaa
      841 tatcttttaa ttttctaatt tttaatcttg aaataggaca acgatggtct tcatagaaaa
      901 caatttctaa gttttttcga tcaatttctc tgatattgcc tatatttacc aaaaatgact
      961 tgtgaggaga ataaaatcgc tgagtatgtt tgtccttttc ctgaatatct gtcatggtac
     1021 cataaaactc ttttgcaaaa ttcttaccaa taatgcgcaa tttatgagat acccctgttg
     1081 tttcaatata caaaatatca tggtaaggaa tttttaaatc atttcccttg taattgtagt
     1141 cgaaataatc tacaacatct tcattttcaa gtaacatact cttcgtgtag aagatatttt
     1201 gctcaattct cttcttaaac atctcatcat tgatatcctt atcaacaaaa tctagggctg
     1261 atacctggta tttataggtt agagtcgcaa actctgatcg actagtgata aagacgataa
     1321 tagcgtaagg attgtaatga cgaatgagct gagccacttc aaatcccttt ttctcaattc
     1381 catgaatatc gatatctagg aaataaagct gatttacttc atcattttca atgtattctt
     1441 caaattcacg gacttttccc gttgtcttgt atgatattgg aatattcgat tctttcgaaa
     1501 tttcatccaa tattctctct agtctcactt gatgttcaat aacatcttct aaaattaaaa
     1561 ctttcattca aattccctct taaatctaat gatttgtcta aatgtactgc cttccatctc
     1621 tgtttctaaa ataatattgt tgtacttatc tagtagttct ttcatattat ttaatccgac
     1681 tccgcgattt cttcccttag tggagaatcc taaggcaaat agatctcctg aaggagtcat
     1741 cgtcatttta catgaattct gaatcaccat aactgtttca gtttccatct taataactgc
     1801 tacttccatc tgctttttat agctatcagc cgatccttcg acagcattat tcaataaaac
     1861 gctcatgata cgaaccaaat ccaatagttc aattggaagc ttggtaatcg tatcttttac
     1921 ttccagtgta aactctacac cattatttcg agcatagaca attgactgag caaccaaact
     1981 tcgtaaagct gagtcttcta tgttgttcaa atcaaagtaa gtgtacttat ctgaacgcaa
     2041 tttatgattt gctttgacta aaacttcatt gtaaattctg tcaatttcct gtaaattacc
     2101 actgtcaatt gccatctgca tgctgacaag cattccagca taatcatgtc gaaaaccacg
     2161 gatttcatta tacagaccaa caatttcatc tgtgtaattc tgtaaatgtt tctgttcaaa
     2221 tttcttctgc ttcaaagcaa tctctttctc catttgaact ttatgagaat tcattgcaaa
     2281 gaaggtcaaa aggagagaga taaagacaat agatgacaaa atacttccaa aactattcaa
     2341 atgtttaatc gtacttacca tatctgaaac gaaagataca atatgtagca atagtaaagc
     2401 aaaaaatact tttttcaaga aaggataaag gtagtccttg tcaaaatagg ctagttccaa
     2461 atggaaataa taaatgattt ttaatgtaac aaaataggtt aacaccgtca caacgaaaaa
     2521 gaatgggaaa tgatattgta aaacaaaatt atctcctgtt atagaggaga aagttacgga
     2581 cagaaagtta tgagtgctct catataaaag agatagtagt aaacttagga atagtcctct
     2641 atccctctca tactgtttca tccatcgaaa ataggaatat aagcccaaag gaaataaaaa
     2701 tctttcaatc cctattttat ctaaatatag aagataaaag gaaaattcaa gtactatttc
     2761 agttagtaat gtataagcac caaaaacgta taattctttt ctatttattc gacctttaca
     2821 aattaaacgg taactgtgac taataattaa aaaatgaaca ataactgtcc caaatccaag
     2881 taaatccatt actctttctc cttatttcat tacttttttc gtaggaaaag aaaatcaagg
     2941 atgattcttg aaatcctcat ctccccacct ttaatatttt gtaagtcttt ttccttcaaa
     3001 gctacaaact gttccaattt aactgtgttt ttcataataa aatctcctaa aatgtttttt
     3061 cttgtaagct aacttacaaa aaccattata caaaatggaa tttcgtttta gataaaattc
     3121 tctcaactgt catttttttc tcccaaagtg tactttttta agaaaaaagc cgggaaaatt
     3181 cccagctttg ctattatatt gatcccagca ggattcgaac ctgcgaccgt tcgcttagaa
     3241 ggcgaatgct ctatccagct gagctatgag atctaataca attattctac caaaaattca
     3301 attaaaagtc aattttctat ttatggtagg ggaatccctg ctgaatcgta aaagcgcgat
     3361 agatttgttc aacaagaact agtctcatta actgatgggg taaggttagg cgaccaaaac
     3421 tgacagaaag attggctcta ttttttacag atgatgacaa tcctaaactt cccccaataa
     3481 taaaagtaag agtagaaaat ccttttatag aagtttcttc taactgctta ctaaattctt
     3541 ctgagaagaa agttttccct tcaatggcta acacaataac gaaatcacgg tcagcaattt
     3601 ttgataaaat tctctgacct tctatttcta aaatcttttg attttctgat tcactggcct
     3661 tatctggtgt tttttcatct gataactcaa tcatttcaaa cttagcaaat ctagaaattc
     3721 gttttgaata ctctgcgata ccatctttta aatacttttc tttcagtttc ccaactgtta
     3781 caactttaat tttcatgact ctattctaac atattctcta ttttttcaca tcttattcac
     3841 aaaataaaaa atagatttca attaagaaaa tcacagtttc aaaagagtta tccacagttt
     3901 gtgtaaaact tttgtgttta agttataatt aagctagtca gtttatactt tcagtaattc
     3961 aaacatatgg aggcaaatat gaaacatcta aaaacatttt acaaaaaatg gtttcaatta
     4021 ttagtcgtta tcgtcattag cttttttagt ggagccttgg gtagtttttc aataactcaa
     4081 ctaactcaaa aaagtagtgt aaacaactct aacaacaata gtactattac acaaactgcc
     4141 tataagaacg aaaattcaac aacacaggct gttaacaaag taaaagatgc tgttgtttct
     4201 gttattactt attcggcaaa cagacaaaat agcgtatttg gcaatgatga tactgacaca
     4261 gattctcagc gaatctctag tgaaggatct ggagttattt ataaaaagaa tgataaagaa
     4321 gcttacatcg tcaccaacaa tcacgttata aatggcgcca gcaaagtaga tattcgattg
     4381 tcagatggga ctaaagtacc tggagaaatt gtcggagctg acactttctc tgatattgct
     4441 gtcgtcaaaa tctcttcaga aaaagtgaca acagtagctg agtttggtga ttctagtaag
     4501 ttaactgtag gagaaactgc tattgccatc ggtagcccgt taggttctga atatgcaaat
     4561 actgtcactc aaggtatcgt atccagtctc aatagaaatg tatccttaag atcggaagat
     4621 ggacaagcta tttctacaaa agccatccaa actgatactg ctattaaccc aggtaactct
     4681 ggcggcccac tgatcaatat tcaagggcag gttatcggaa ttacctcaag taaaattgct
     4741 acaaatggag gaacatctgt agaaggtctt ggtttcgcaa ttcctgcaaa tgatgctatc
     4801 aatattattg aacagttaga aaaaaacgga aaagtgacgc gtccagcttt gggaatccag
     4861 atggttaatt tatctaatgt gagtacaagc gacatcagaa gactcaatat tccaagtaat
     4921 gttacatctg gtgtagttgt tcgttcggta caaagtaata tgcctgccaa tggtcacctt
     4981 gaaaaatacg atgtaattac aaaagtagat gacaaagaga ttgcttcatc aacagactta
     5041 caaagtgctc tttacaacca ttctatcgga gacaccatta agataaccta ctatcgtaac
     5101 gggaaagaag aaactacctc tatcaaactt aacaagagtt caggtgattt agaatcttga
     5161 ttgacatcta tgtaaagaaa gctttacata agagaaaaga tgtgttagtg tagaatcatg
     5221 gaaaaatttg aaatgatttc tatcacagat atacaaaaaa atccctatca accccgaaaa
     5281 gaatttgata gagaaaaact agatgaacta gcacagtcta tcaaagaaaa tggggtcatt
     5341 caaccgatta ttgttcgtca atctcctgtt attggttatg aaatccttgc aggagagaga
     5401 cgctatcggg cttcactttt agctggtcta cggtctatcc cagctgttgt taaacagatt
     5461 tcagaccaag agatgatggt ccagtccatt attgaaaatt tacagagaga aaatttaaac
     5521 ccaatagaag aagcacgcgc ctatgaatct ctcgtagaga aaggattcac ccatgctgaa
     5581 attgcagata agatgggcaa gtctcgtcca tatatcagca actccattcg tttactttcc
     5641 ttgccagaac agattctttc agaagtagaa aatggcaaac tatcacaagc ccatgcgcgt
     5701 tccctagttg ggttaaataa ggaacaacaa gactatttct ttcaacggat tatagaagaa
     5761 gatatttctg taaggaaatt agaagctctt ctgacagaga aaaaacaaaa gaaacagcaa
     5821 aaaactaatc atttcataca aaatgaagaa aaacagttaa gaaaactact cggattagat
     5881 gtagaaatta aactatctaa aaaagacagt ggaaaaatca ttatttcttt ttcaaatcaa
     5941 gaagaatata gtagaattat caacagcctg aaataaggct gttcttttat ttttttatct
     6001 cacaaggtta tccactatgt ttttcgataa aaagcttaat aaatcaataa tttcttcttt
     6061 tatccccaac ctgtggataa agtttggtaa cattgtggat tatttttcac agcttgtgga
     6121 aaattcttgc tatctatggt aaaatatctc tagtattaaa cttttaaata gtaaaggagg
     6181 agaaaggatt gaaagaaaaa caattttgga atcgtatatt agaatttgca caagaaagac
     6241 tgactcgatc catgtatgat ttctatgcta ttcaagctga actcatcaag gtagaggaaa
     6301 atgttgccac tatatttcta cctcgctctg aaatggaaat ggtctgggaa aaacaactaa
     6361 aagatattat tgtagtagct ggttttgaaa tttatgacgc tgaaataact ccccactata
     6421 ttttcaccaa acctcaagat acgactagct cacaagttga agaagctaca aatttaactc
     6481 tttataacta tagtccaaag ttagtatcta ttccttattc agatacggga ttaaaagaaa
     6541 agtatacctt tgataacttt attcaagggg atggaaatgt ttgggctgta tcagccgctt
     6601 tagctgtctc tgaagatttg gctctgacct ataaccctct ttttatctat ggaggaccag
     6661 gccttggtaa gactcactta ttaaacgcta ttggaaatga aattctaaaa aatattccta
     6721 atgcgcgtgt taaatatatc cctgccgaaa gctttattaa tgactttctt gatcacctaa
     6781 gacttgggga aatggaaaag tttaaaaaga cctatcgtag tcttgatctt ttgttaatcg
     6841 atgatattca gtcactcagc ggaaaaaaag ttgcaactca ggaagaattt ttcaatacct
     6901 ttaacgccct tcatgacaag caaaaacaga ttgtcctaac gagtgatcgt agtccaaaac
     6961 atctagaagg gctcgaggag aggcttgtca cgcgttttag ttggggattg acacaaacta
     7021 tcacaccccc tgactttgaa acacgtattg ccattttaca aagtaaaacg gaacatttag
     7081 gctacaattt ccaaagtgat actctagaat acctagctgg gcaatttgat tcaaatgttc
     7141 gagatcttga gggagccatc aacgacatca ctttaattgc cagagtaaaa aaaatcaagg
     7201 atatcactat tgatattgct gcagaagcca ttagagcccg caaacaagat gttagccaaa
     7261 tgctcgtcat cccaattgat aaaatccaaa ctgaagttgg taacttttat ggtgttagta
     7321 tcaaagaaat gaagggaagt agacgccttc aaaatattgt tttggcccgt caagtagcca
     7381 tgtatttatc tagagaacta acagataata gtcttccaaa aattgggaag gaatttgggg
     7441 gaaaagatca taccacagtc attcatgccc atgccaaaat aaaatctttg attgatcaag
     7501 acgataattt acgtttagaa attgaatcaa tcaaaaagaa aatcaaataa tttgtggata
     7561 acttttagtt ttttatcttt tttatccaca ttttttaaac aagctaaaaa acttgatatg
     7621 acttgtttaa aggctgtttt ccacagattt cacagactct attattacta ttatctttct
     7681 aatactaaaa ataaataaag gagaatccat gattcatttt tcaattaata aaaatttatt
     7741 tctacaagca ttaaatacta ctaagagagc tattagttct aaaaatgcca ttcctatttt
     7801 atcaacagta aaaattgacg tgaccaatga aggtattact ttaattggtt caaatggtca
     7861 aatttcaatt gaaaatttta tttctcaaaa aaatgaagat gctggtttgt taattacttc
     7921 tttaggttcg atccttcttg aagcttcttt ctttatcaat gtagtatcta gtttacctga
     7981 tgtgactctt gattttaaag aaattgaaca aaatcaaatt gttttaacca gtggcaaatc
     8041 agaaattacc ctaaaaggaa aagatagcga acaatatcca cgaatccaag aaatttcagc
     8101 aagcactcct ttaatacttg aaacaaaatt actcaagaaa attattaatg aaacagcctt
     8161 tgctgcaagt acacaagaga gtcgtccgat tttaacaggt gtccacttcg tattgagtca
     8221 acacaaagag ttaaaaacag ttgcaacaga ctctcatcgc ctaagccaga aaaaattgac
     8281 tcttgaaaaa aatagtgatg attttgatgt cgtaattcct agccgttctc tacgcgaatt
     8341 ttcagcggta tttacagatg atatcgaaac tgtagagatt ttctttgcca ataaccaaat
     8401 cctctttaga agcgaaaata ttagcttcta tactcgtctc ctagaaggaa actatcctga
     8461 tacagatcgc ttgattccaa cagactttaa cactactatt acttttaatg tggtaaactt
     8521 acgccagtca atggagcgtg cccgtctttt atcaagtgcg actcaaaatg gtactgtgaa
     8581 acttgaaatt aaggatgggg ttgttagcgc ccatgttcac tctccagaag ttggtaaagt
     8641 aaacgaagaa atcgatactg atcaggttac tggtgaagat ttgaccatta gtttcaaccc
     8701 aacttacttg attgattctc ttaaagcttt aaatagcgaa aaggtgacca ttagctttat
     8761 ctcagctgtt cgtccattta ctcttgtgcc agcagatact gacgaagact tcatgcagct
     8821 cattacacca gttcgtacaa attaagtgaa agaggttgag cctggctcgc ctcttttatg
     8881 atataatcga aaaagaaaag gagagtagta tgtatcaagt tggaaatttt gttgagatga
     8941 aaaaatcaca cgcttgtaca atcaagtcga ctggtaaaaa ggctaatcgt tgggaaatta
     9001 cacgtgtagg agcagatatc aaaataaaat gcagtaattg tgagcatgtt gtcatgatgg
     9061 ggcgatatga ttttgagcga aaaatgaata aaattattga ctgagaaccc ttagttagag
     9121 ggttagcgct ttatcccttt ttgtgttata atattaggga ttgaaatgaa aacggagaat
     9181 gagaaatatg gctttgacag caggtatcgt tggtttgcca aacgttggta aatcaacact
     9241 atttaatgca attacaaaag caggagcaga ggcagcaaac tacccatttg cgacgattga
     9301 tccaaatgtt ggaatggtgg aagttccaga tgaacgccta caaaaactaa ctgaaatgat
     9361 aactcctaaa aaaacagttc ccacaacatt tgaatttacg gatattgcag ggattgtaaa
     9421 aggagcttca aaaggagaag ggctagggaa taaattcttg gccaatattc gtgaagtaga
     9481 tgcgattgtt cacgtagttc gtgcttttga tgatgaaaat gtgatgcgcg agcaaggacg
     9541 tgaagacgcc tttgtagatc cacttgcaga tattgataca attaatctgg aattaattct
     9601 tgctgactta gaatcagtga acaaacgata tgcgcgtgta gaaaagatgg cacgtacgca
     9661 aaaagataaa gaatcagtag cagaattcaa tgttcttcaa aagattaaac cagtcctaga
     9721 agacgggaaa tcagctcgta ccattgaatt tacagatgag gaacaaaagg ttgtcaaagg
     9781 tcttttcctt ttgacgacta aaccagttct ttatgtagct aatgtggacg aggatgtggt
     9841 ttcagaacct gactctatcg actatgtcaa acaaattcgt gaatttgcag cgacagaaaa
     9901 tgctgaagta gtcgttattt ctgcgcgtgc tgaggaagaa atttctgaat tggatgatga
     9961 agataaaaaa gagtttcttg aagccattgg tttgacagaa tcaggtgtag ataagttgac
    10021 gcgtgcagct taccacttgc ttggattggg aacttacttc acagctggtg aaaaagaagt
    10081 tcgcgcttgg actttcaaac gtggtatgaa ggctcctcaa gcagctggta ttatccactc
    10141 agactttgaa aaaggcttta ttcgtgcagt aaccatgtca tatgaagatc tagtgaaata
    10201 cggatctgaa aaggccgtaa aagaagctgg acgcttgcgt gaagaaggaa aagaatatat
    10261 cgttcaagat ggcgatatca tggaattccg ctttaatgtc taaaaattaa taaatggtgt
    10321 caattaggtt ggaaaaaaaa ttccaaccct tttggctttt gaaaggaaaa ataaatgacc
    10381 aaattacttg taggcttggg aaatccaggg gataaatatt ttgaaacaaa acacaatgtt
    10441 ggttttatgt tgattgatca actagcgaag aaacagaatg tcacttttac acacgataag
    10501 atatttcaag ctgacctagc atcctttttc ctaaatggag aaaaaattta tctggttaaa
    10561 ccaacgacct ttatgaatga aagtggaaaa gcagttcatg ctttattaac ttactatggt
    10621 ttggatattg acgatttact tatcatttac gatgatcttg acatggaagt tgggaaaatt
    10681 cgtttaagag caaaaggctc agcaggtggt cataatggta tcaagtctat tattcaacat
    10741 ataggaactc aggtctttaa ccgtgttaag attggaattg gaagacctaa aaatggtatg
    10801 tcagttgttc atcatgtttt gagtaagttt gacagggatg attatatcgg tattttacag
    10861 tctattgaca aagttgacga ttctgtaaac tactatttac aagagaaaaa ttttgagaaa
    10921 acaatgcaga ggtataacgg ataaatggtg accttattag atttattctc agaaaatgat
    10981 cagattaaaa aatggcatca aaatttaaca gataagaaaa gacaactaat acttggttta
    11041 tcaacatcta ctaaggctct tgcaattgca agcagtttag aaaaagaaga taggattgtg
    11101 ttattgacgt caacttatgg agaagcagaa ggacttgtta gtgatcttat atctatcttg
    11161 ggtgaggaac tcgtctatcc atttttggta gatgatgctc ctatggtgga gtttttgatg
    11221 tcttcacagg aaaaaattat ttcacgggtt gaagccttgc gttttttgac tgattcatct
    11281 aagaaaggga ttttagtttg taatatcgta gcaagtcgat tgattttacc gtctcccaat
    11341 gcattcaaag atagtattgt aaaaatctca gttggtgaag aatatgatca acacgcgttt
    11401 atccatcagt taaaggaaaa tggctatcga aaagttactc aagtacaaac tcagggcgaa
    11461 tttagtcttc gaggagatat tttagatatt tttgaaatat cccagttaga accttgtcga
    11521 attgagtttt ttggtgatga aattgatggt atcaggtcat ttgaagtaga aacacaatta
    11581 tcgaaagaaa ataagacaga actcactatc tttccagcta gtgatatgct tttgagagaa
    11641 aaggattatc aacgaggaca gtcagcttta gaaaaacaaa tttcaaaaac tttatcacct
    11701 attttgaaat catacctaga agaaattctt tcaagttttc accaaaaaca aagtcatgca
    11761 gactctcgga agtttttatc tttgtgctat gataagacat ggactgtctt tgattatatt
    11821 gaaaaagata ccccaatatt ctttgatgat tatcaaaaat tgatgaatca gtatgaagtc
    11881 tttgaaagag acttagcgca gtactttaca gaagaattac agaatagtaa agcattttct
    11941 gatatgcagt atttttctga tattgaacaa atctataaaa aacaaagtcc agtgaccttt
    12001 ttctctaatc ttcaaaaggg tttaggaaat ctcaaatttg acaaaattta tcaattcaat
    12061 caatatccta tgcaggaatt tttcaatcag ttttcttttc taaaagaaga aattgaacga
    12121 tataaaaaaa tggattacac cattattctg cagtctagca attcaatggg aagtaaaaca
    12181 ttggaggata tgttagagga atatcagatt aaattggatt ctagagataa gacaaatatc
    12241 tgtaaagaat ctgtaaactt aatagagggt aatctcagac atggttttca ttttgtagat
    12301 gaaaagattt tattgataac tgaacatgag atttttcaaa agaaattaaa gcgtcgtttt
    12361 cgaagacaac atgtttcaaa tgcagagaga ttaaaagatt acaatgaact tgaaaaaggg
    12421 gactatgttg tccatcatat ccatgggatt ggtcaatatc taggaattga aaccattgaa
    12481 atcaagggaa ttcatcgcga ttatgtcagt gtccaatacc aaaatggtga tcaaatttct
    12541 atccccgtgg aacagattca tctactgtcc aaatatattt caagtgatgg taaagctcca
    12601 aaactcaata aattaaatga cggtcatttt aaaaaggcca agcaaaaggt taagaaccag
    12661 gtagaggata tagctgatga tttaatcaaa ctctactctg aacgtagtca gttgaagggt
    12721 tttgctttct cagctgatga tgatgatcaa gatgcctttg atgatgcttt cccttatgtt
    12781 gaaacggatg atcaacttcg tagtattgag gaaatcaaga gggatatgca ggcttctcag
    12841 ccaatggatc gacttttagt tggggatgtt ggttttggaa agactgaagt tgctatgcgt
    12901 gcagccttta aagcagtcaa tgatcacaaa caggttgtca ttctagttcc gacgacggtt
    12961 ttagcgcaac agcactatac gaattttaag gaacgattcc aaaattttgc agttaatatt
    13021 gatgtgttga gtcgctttag aagtaaaaaa gagcagactg caacacttga aaaattgaaa
    13081 aacggtcaag tcgatatttt gattggaaca catcgtgttt tgtcaaaaga tgttgtgttt
    13141 gctgatttgg gcttgatgat tattgatgag gaacagcgat ttggtgtcaa gcataaggaa
    13201 actttgaaag aactgaagaa acaagtggat gtcctaacct tgaccgctac gccaatccct
    13261 cgtaccctcc atatgtctat gctgggaatc agagatttat ctgttattga aactccgccg
    13321 actaatcgct atcctgttca gacctatgtt ttggaaaaga atgatagtgt cattcatgat
    13381 gctgtcttgc gtgaaatgga gcgtggaggt caagtttatt atctttacaa caaagttgac
    13441 acaattgttc agaaggtttc agaattacag gagttgattc cggaggcttc gattggatat
    13501 gttcatggtc gaatgagtga agtccagttg gaaaatactc tattagactt tattgaggga
    13561 caatacgata tcttggtgac gactactatt attgagacag gggtggacat tccaaatgct
    13621 aatactttat ttattgaaaa tgcggaccat atgggcttgt caaccttata tcagttaaga
    13681 ggaagagtcg gtcgtagtaa tcgtattgct tatgcttatc tcatgtatcg tccagaaaaa
    13741 tcaatcagtg aagtctctga aaagagatta gaagcgatta aaggatttac agaattgggc
    13801 tctggcttta agattgcaat gcgagatctt tcgattcgtg gagcaggaaa tcttttagga
    13861 aaatcccagt ctggtttcat tgattctgtt ggttttgaat tgtattcgca gttattagag
    13921 gaagctattg ctaaacgaaa cggtaatgct aacgctaaca caagaaccaa agggaatgct
    13981 gagttgattt tgcaaattga tgcctatctt cctgatactt atatttctga tcaacgacat
    14041 aagattgaaa tttacaagaa aattcgtcaa attgacaacc gtgtcaatta tgaagagtta
    14101 caagaggagt tgatagaccg ttttggagaa tacccagatg tagtagccta tcttttagag
    14161 attggtttgg ttaaatcata cttggacaag gtctttgttc aacgtgtgga aagaaaagat
    14221 aataaaatta caattcaatt tgaaaaagtc actcaacggc tgtttttagc tcaagattat
    14281 tttaaagctt tatccgtaac gaacttaaaa gcaggcatcg ctgagaataa gggattaatg
    14341 gagcttgtat ttgatgtcca aaataagaaa gattatgaaa ttttagaagg tctgctgatt
    14401 tttggagaaa gtttattaga gataaaagag tttaaggaag aaaa
//
LOCUS       DAVMGZ010000040        14133 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000040 DAVMGZ010000000
VERSION     DAVMGZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14133
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.041"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(241..1848)
                     /locus_tag="VYQ93_000999"
     CDS             complement(241..1848)
                     /locus_tag="VYQ93_000999"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV3335701.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2238..2825)
                     /locus_tag="VYQ93_001000"
     CDS             complement(2238..2825)
                     /locus_tag="VYQ93_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV3335702.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2953..3402)
                     /locus_tag="VYQ93_001001"
     CDS             complement(2953..3402)
                     /locus_tag="VYQ93_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3335703.1"
                     /translation="MCFVPYYKVNYCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASSRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3407..3796)
                     /locus_tag="VYQ93_001002"
     CDS             complement(3407..3796)
                     /locus_tag="VYQ93_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV3335704.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3824..4450)
                     /locus_tag="VYQ93_001003"
     CDS             complement(3824..4450)
                     /locus_tag="VYQ93_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV3335705.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGALFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4464..5027)
                     /locus_tag="VYQ93_001004"
     CDS             complement(4464..5027)
                     /locus_tag="VYQ93_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3335706.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5192..5426)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5480..5983)
                     /locus_tag="VYQ93_001005"
     CDS             complement(5480..5983)
                     /locus_tag="VYQ93_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV3335707.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6176..7006)
                     /locus_tag="VYQ93_001006"
     CDS             complement(6176..7006)
                     /locus_tag="VYQ93_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV3335708.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQTLKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6991..8673)
                     /locus_tag="VYQ93_001007"
     CDS             complement(6991..8673)
                     /locus_tag="VYQ93_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3335709.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLQTELVKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8775..9032)
                     /locus_tag="VYQ93_001008"
     CDS             complement(8775..9032)
                     /locus_tag="VYQ93_001008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335710.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9039..9587)
                     /locus_tag="VYQ93_001009"
     CDS             complement(9039..9587)
                     /locus_tag="VYQ93_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV3335711.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9602..10450)
                     /locus_tag="VYQ93_001010"
     CDS             complement(9602..10450)
                     /locus_tag="VYQ93_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV3335712.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10639..11988
                     /gene="trkA"
                     /locus_tag="VYQ93_001011"
     CDS             10639..11988
                     /gene="trkA"
                     /locus_tag="VYQ93_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV3335713.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11992..13431
                     /locus_tag="VYQ93_001012"
     CDS             11992..13431
                     /locus_tag="VYQ93_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV3335714.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13556..13813)
                     /locus_tag="VYQ93_001013"
     CDS             complement(13556..13813)
                     /locus_tag="VYQ93_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEV3335715.1"
                     /translation="MNEGAQLAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYRE
                     MKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
BASE COUNT         4149 a         3228 c         2599 g         4157 t
ORIGIN      
        1 tcactataca atccagttcc taaaactttg ctagaaattg atttggcttt ccgaattaat
       61 ttgttcaaat tttatttcat ttcaatatac tgataaatcc attcactagc acagggcaag
      121 agggagtcgc tattgatttc aacggtagca ggtacataca gaggactcct acctcagata
      181 tactgcaata tcatcgtatg caaaaaacct ctgagatttt tctcaaaggt tctgattttg
      241 ctaattgctg ttctcaaccg ctgcagtaac aaaggcagtg tagagttctt ctggtcggtt
      301 tggacggctt gacagttcag ggtgatactg acaagctaca aagaatttat tttcaggaat
      361 ttccacgatt tctaccaaac gattgtctgg agaaactcct gaaaagacaa aacctgctgc
      421 ctcaaactgc tcacggaagg cattattaaa ctcataacgg tgacggtgac ggcgttgcac
      481 cacttcttga ttgtgataag cagcagcagc cttagagcca cgtttcaact tagacggata
      541 aagtcccaaa cgaagggttc cacccatatc ctcaatatca atctgatcac gcatgatatc
      601 aatgataggg tattttgttt ctggtgcaag ctctgcagaa ttggcacctt caagacctaa
      661 aacgtgacga gcaaactcga tacatgtcaa ctgcattccc aagcagactc ccaacattgg
      721 aacatcattt tcacgcgcat agcggatggc ttggattttc ccttctgtac cacgttgacc
      781 aaaaccacct ggtacgatga tcccgtccgc atcagacaag agttctgcta cattctctgc
      841 tgtcacatca ttggcattga cccaattgat tttaacttct acatcattga catagccaga
      901 gtgtttcaag gcttcgacca ctgagatata ggcatcttgc aactccacat acttaccaac
      961 aagggaaatc ttaacttgtt tcttgaggtt catgaccttg tccaccatgg ctgaccattc
     1021 tgtcatatcc gctgctggtg cgtctaattt caaatgatca caaacaattt ggtccatccc
     1081 ttgtgcctgc aagttcagtg gaatttggta aaggtgttca acatccaacg attcgataac
     1141 ggcttctggt gccacatcac agaactgggc cagtttattt ttaattcctt gaccagctgg
     1201 ctcttctgta cgaataacca acatatttgg ttggattccc aagccacgca attctttgac
     1261 agagtgttgg gttggtttgg ttttcatttc accagcagcc ttgaggtaag gaagcaaggt
     1321 tgtatggata tacatgacat tatccgcacc cacatctgcc ttcatctgac gaagagcctc
     1381 taggaatggc aaggactcga tatctcctac tgttccacca acctctgtga taatgacatc
     1441 agagtcggtc gttagagcgg cacgcttgat tttttctttc aaagcatctg tgatatgagg
     1501 aatgacttga acagttgccc caaggtattc tccacggcgt tctttacgaa gaacttcact
     1561 gtaaattttc ccagttgtca cgttggaata tttgttgaga ttgatatcga tgaaacgttc
     1621 atagtgaccc aagtccaaat ctgtctcagc tccgtcatct gtcacaaaaa cttccccgtg
     1681 ctggtaagga ctcatggttc ccggatcaat attgatataa gggtcaaact tttgaatggt
     1741 tactttgaga ccacgatttt tcaagagacg gcctagactc gctgccacaa tccctttccc
     1801 aatggacgat accacaccac cagttacaaa aatatatttc gtagacatag attcctcttt
     1861 ctaaaatgct caaggtcttg ttaactacga ggggatatag aaaacaagac cctactaata
     1921 agaataatct gggacgactg caccagattc acaactaata ctcttcgaaa atctcttcaa
     1981 accgcgtcaa cgtcgccttg ccgtagatat gtgttcctga ctttgtcagt cttatctaca
     2041 acctcaaaac agtgttttga acagcctacg gctagtttcc tagcttgctc tttgattttc
     2101 attgagtata aaatacaaga agataacttc ttgaaaaaac aaaaatagct ccctaagaac
     2161 tagggagccc cgacctctaa aagaggtgcc caaacaatat gatacctaaa aataggataa
     2221 ttgtcaatag ctaactctta ttcctcgctg gtttcagcgt cgtcatctga aaactcgtcg
     2281 tcgtcttcgt tgagatccac atcttctccc aagtcatctg gagcgatttc gttgatttct
     2341 gcatcataag cttccacttc atttttttca tcatctggat tttcatcatc gtatgaaaga
     2401 gctggatctg cttcgtatgc atcttcgtct tctggatcat ctgcattgta gtcaatggca
     2461 tctgaatcac catccataaa ggcattgaca cgttttttct tagcttttgg tgctacttca
     2521 tcgtcgtcat tttcttcaag agcgatgatt tcttcgtcga tttcgtccac accataccat
     2581 gaacgaagac cccatttgtt gtccccaagt gagatgaagc taccgtcaaa gttcaactct
     2641 gtgtagaaca aaggcaaagc ttcgcggata tcgctgtttg atgttccaag gtagttttga
     2701 atttcgttta caagatcgct aaaatgcatc tcatgatcgc gaccacgaag ttccaagata
     2761 gcacgcgcta cctcaatcat agatagttca cttttttctt gcccagcaaa tacttctaat
     2821 tccaaagcgt ttctcctcat ttatactact atcgccagag cgaacagact ctgacctcat
     2881 tttatcattt actctttatt ttacgataat tttgcggaat agtcaaaggt taagggggag
     2941 aaagtggcag gattagacta attccaatat aaaactcctt cctttttctg ttgctccatt
     3001 ttccacaaat ccaagcgact tgaaacacct actagaagca tgattgtagg tgtagatttc
     3061 cttgactctc aattctttcc atccttttac tcgagccaat tcaatcaaag cacttagaat
     3121 cttttttcca agtcctcgat gttggtaagc ggaattccca atcacaatgg ggagattatc
     3181 ctgagatagt gtaatatccc caattggaaa ccattctccc ttctccttga cttcaatcca
     3241 aaaaagctca ccatgccgat ccaaatagga atacatggct tccaaggtcg cttgactgta
     3301 aggaagcttc accccatcta cgaggtaaac caagttcaca tcctgatacc aagcaaaagc
     3361 ttcctcacaa taattgacct tataataagg aacaaaacac aatgcactac cttttcaaca
     3421 aaagagttgc tgcttgatta aaaccatcac accagttata ccattttgct tcatacccat
     3481 cttgagctag gatacgatct tctaaatcaa aaacagagta aatctttctt tcctcgcaag
     3541 cttgcgcata gagatgatat agttcatcac caccatctct atcccactca gcagaaatcg
     3601 tatcccgacc tgccaataaa gcctgataag ccctgtgatg cccatctgta atcagcaaac
     3661 aatctccaaa ggcaagaata ctgattggat cgacttggat tgtttctgcc gactggtaaa
     3721 gcatctgaat atcttgcaac ttcttttctg ataaatatag ttgagtcgga tgaagatctg
     3781 ctatattgac tttcatttct ttctcctcaa gggaattcga tactcacttc tgtttgcctt
     3841 taaatcgcca ttggaagcgg agcttatcat aaaagggaaa ctcgataaac agggctccca
     3901 agcccacaca gagactggca agaacatctg atgggtaatg aactcccaga tagactcttg
     3961 ataccagcac actgactagg tagaggccaa ggacgatttg tacgattttt ctccagacct
     4021 gatctttaat ccgctgacta agaataacaa tcaaagtccc taccatcagc gttacagcca
     4081 gagaatgccc acttgggaag gaaaatccct tctcctccac cagatgtaag atagctggtc
     4141 gtgggcgctg gtagatattt ttaaaggtca cgattaaaag acctgccaaa gccagatttc
     4201 ccagcatgaa gaaactttct atcttccatc gcttacgata aaagacaaaa gctataatga
     4261 caacccaagt gataatcact ggaatatcaa tcagacgtgt gagggatcga aaaagaatag
     4321 tcaaataatc tggtaagtct cctcgaatgg cagtctgaat cgattggtca aaattgacca
     4381 acatttcagg gtaaaatttg accatatagc caagaataac gaaaagtaaa agggcaaaac
     4441 tgcccttcat taaaaatgtt tgtttatctt tcatagtgtt ttaaggttgg tttcaaaagg
     4501 acgtacaaca accagaatga aacggaaaag attacaccct caatcaagtt aaaaggtaat
     4561 accatggtca ttaggtagtt ggaaagtccc aaaatttttc caatatcaaa gttagcaaac
     4621 ttagcgtaca aaggaacagc ataaacatag ttgagaacca acatggccaa ggttaaacca
     4681 acagttccag ctagagagcc tagtaggaaa cgaagggttg tccgttcctt tttccaaatc
     4741 aaagcaaata cgatgacaaa aactcccaaa gctacgatat tcatcggcaa accaatgtaa
     4801 gtattcactc cttggctgtt aagaagcaat ttcaatagtg agcgaagcaa gagcactcct
     4861 agagcagcag gcaaatccat gaccaccaga cccacaagga ctggcaagat actaaattcg
     4921 atcttgagga aagatgccgc tggtaaaagc ggaaagtcaa agtacatcag cacaaatgag
     4981 atggctgata aaattgcaat ggtcgaaagt cgacgtgtgt ttgtcataac aggttcctcc
     5041 aattttctat aaaatcagaa gaagttggaa aggattcctc tatctattct cactttttat
     5101 atcccaaaag ttccctctta ctctattaaa gaaaaacaaa gcaagtggtt acaatccggc
     5161 tataaatcta tcaaaacaga caaagctatt ctttcgtctt ctcccatcca gactatactg
     5221 tcggttgtgg aatctcacca catcagcttg cgctcgcgga cttctttaaa gagaaggaaa
     5281 tagagatttt tcttttagac taggcgacaa gctgtaggca taacttctgg ttaggcaaag
     5341 cttgtcaaca aagtataaaa ggaaaagatc atttcctagt taaagtcacc gccggtcggg
     5401 aatcacaccc tgccctgaag actcttttat cataacaaaa aacgcttgca agcgcaagca
     5461 ttttgttcat tttcattttt tatttcaatt tatccacttc ataggtatgg acgagctcaa
     5521 gacctgcaaa gttctgctgg cgaagagcct catagacaat catgcagacg gtattagaca
     5581 cattgagact gcggacatgt tcatcattca tgggaatacg gagagccttc tcaggatgtt
     5641 ctcgcataaa atcttcaggt aagcccttgt cttcacgtcc aaagagaaaa taatggtcct
     5701 cgtcagtcga taaatccacc tcagaataga ctttttccgc gaatttcgaa atcagataga
     5761 gttttccctt catctgagat atgaaatctt ccaaactctc gtaaaaataa atctctagtt
     5821 tatcccagta atccaatcca gcccgcttca tcttgcggtc atcaatagga aagcccatag
     5881 gcttgatgat gtggagggga gaattggtcg cagcgcaagt acgcgcgata ttgcccgtat
     5941 tttgtggaat ttgaggttca aataatacaa tgtgatttgt catgacttgc ttcctttcac
     6001 cattgcaaaa aaatagccac actgcccgga gtcaagctca gcaaacagcg tggttaaggc
     6061 atcgttaact tacctcacaa caggtttgaa gtaaatcagc gaaactactt tcttagtata
     6121 acactttcag aatcattgtc aatagaaatg acttgatttt ttcaattttt tcaagctatt
     6181 tccaagggtt gtaaaatcgt ccctgattct gcaagataag tagtaaacta actactaaaa
     6241 acaaggttgc caagagcaag gtaatatagt ctcctttttt caaggtctga taactatacc
     6301 atgtgcgttt tttctctttc ccaaagcggc gaagctccat ggcagtcgca atagtatcaa
     6361 tgcgttctag cgagctaaaa atcaagggcg taataatgag cagattgcct ttgattcgtt
     6421 gcataagaga agctttcttg gataattcca tcccacgcgc ctcctgagac atcttgatag
     6481 taaagaattc ttcctgcaaa tctggaatat agcgcaaggt caggctgaca gaataagcaa
     6541 tcttataggg cacaccaatt tgatttaaac tggaagcaaa ctgactagga tgggttgtca
     6601 tcaaaaagat aatagccaga ggaatggtgc aaagatactt aatggccaaa tttagcagat
     6661 aaaagagctc ctggctggtt agagtgtaga caccgattcc ctgccaaatc acacttctct
     6721 ctccataaag tccaacccca tactcgggag aaaagagata gaccatcaaa acgtttaaaa
     6781 cggcaaatac cgtcgcaaaa acggctacaa aggaaacatc tttaaagcga atttctgata
     6841 aatagaggag aaagactgaa aagatggcaa tcagcaagag cattctggtg tcatagctaa
     6901 tcatggccgc caatgatacc agaatgaaaa agagaagttt cccagctcct gacaagcgat
     6961 gaatcacagt atctctatgc tggtaaccga ttaatttagc ttgcatccct ctctcctttc
     7021 tttgtaaaat gccgttaaat ccagtggatc cacatctagt ttcttagcca agttaaagat
     7081 ggaggtttct tttagattgg cttttactaa cagctcagga tcgctcaaca gactggctgg
     7141 aacagtatcg gcaatcaatt ctccatccac catgacaagg acccggtctg aataatccag
     7201 catcaattgc atatcatggg taatcatgac aatggtatgc cctttttgat gtaactcttc
     7261 gagaaattcc ataatctcag tatagttctt ctgatcttga cctgcagtcg gttcatctag
     7321 gagaataatt tcagctccta agaccaaaat tgaagcaatg gtgacacgtt ttttctgacc
     7381 aaatgacagg gcagaaatag gccaattacg gaattcataa agtccacaga ttttcaaggt
     7441 ttcatagact ctcgtttcaa tttccttctc atccacacct cgcaaacgga gccctagagc
     7501 cacctcatca aaaatcatat tggttgaaat catttgatta ggattttgta gcacatagcc
     7561 tactcgttcc gcccgctctg caacagaatc gccttttata tcctgttttt cccaaagata
     7621 gcgtccttcc gtctgaataa agctacttat agccttggct agagttgatt tccctgctcc
     7681 attttttccg acaatagcaa tcttttcacc ctttttaata tctaaatgta gggattttaa
     7741 aatcggtcta tcatcataag aaaaagatac ttcctctagt ctaaagagtg actgcaatgc
     7801 tggggtttct tttaccagtt ccgtctgtaa ctggacttgc ccttttgaga tagacaagtt
     7861 atccagatct gctaattgtt cttccttgac taagtccaca cctaattgac ggagagtcgt
     7921 tagataaagg ggttctcgaa ttccattttg agtcaataaa tcagtcgcaa gcaactggtc
     7981 agggctccca ttaaaaagga tacgaccatc gtttatcaag acaatccgat ccacagggcg
     8041 atgcagaacg tcctccaaac ggtgctcgat aatcagagtc gtcgtcccct cttccttatg
     8101 aatctggtca atcaattcga taatatcctg acctgacttg ggatctagat tggcgagtgg
     8161 ctcatcaaac aagagaatcg gactttcatc aatcaagaca ccagccagac tgactcgctg
     8221 cttttgtcca cctgacaaat cctgaggacg ctgatccagt aaaggaagaa ggtccagctt
     8281 ttcagcccat ttataaacac gacctttcat ctcatctagg gctgtcacat cattttccag
     8341 agcaaacgcc aaatcttctg ccacagacaa gccaataaac tgcccatctg tatcctgcaa
     8401 aactgtgcta accagatgag acttatcata gatgctcata tcaaaggctg tttgaccctt
     8461 tatcaaaaat tctccatatg tctgaccctt gtaaatattg ggaataatcc cattcaaaca
     8521 ctgacccaag gtagatttac ctgatccaga tggtccaaca attaagactt tttctccctt
     8581 gtaaatggtc aagtctatcc cttgcaaggt cggttcttgt tgtgtttcat accggaaaga
     8641 gaaatccttc cactcaatta tagcttcttt catcttactc tcttcattcg cttcttagac
     8701 ttctatttta tcataaatca agcccttctt gcagtctctt ctcttaaaat cttagcgcca
     8761 aaaagattcc tatcttagct tacttgccta actaatctat aaacatcgaa aaagactagt
     8821 tgcccagcct tccccatcat tttatactct tcgaaaatct cttcaaacca cgtcagcttc
     8881 gccttgccgt aggtatggtt actgacttcg tcagtttcat ctacaacctc aaaaccatgt
     8941 tttgagctga cttcgtcagt tctatccaca atctcaaaac actgttttga gcaacctgcg
     9001 gctaacttcc tagtttgctc tttgattttc attgagtatt agtccttttt caaacttcct
     9061 gcacgagttt gagttcctgc ataggcaagt aagagaagag ttcctgcaat agctacagat
     9121 acaccattgg caattcccgc aacaatccct tgtgcaaata ctttttctgc cgcttcttga
     9181 taaatcacaa catctccaag tggtgccaag acaccccaaa caagggcatt tgcaagtagt
     9241 tgaatgaggt taaaaataag aatatctttc cagtcaaaaa caccattgat cactcgaacg
     9301 tactttctaa aaagtcccac aactaaacca aagagtccgc tagcgataat ccaagtccac
     9361 catagaccat aaccagcaag agagtccttg attgcatgac caatcaaccc gacaagcaaa
     9421 ccgataatcg gtccaaaaat aatagaaagt agcgcttgta ccgcatactg aagctggatg
     9481 cttgtatttg gaacaggggt tggaatgttg atcatcccga tgacgacaaa gagggcagcg
     9541 ccaattccga cagcaacaac ttgtttaatt gtaaatttga tttccatact attctcctat
     9601 tttatccttc tattttcttt atttcaatgg tccaagatga accgacacct acattatagg
     9661 ccttggcaaa ggaaccttgg ttgatagcca aacctaaacg atagagagag ttgatgtaaa
     9721 ggatgggttg cccaattctc acatctgcaa atgatttgcc atagacaacc tgattttgat
     9781 agaccagcat atcagcatga tagatggtca cttcaaaacg atcaccaaat tctggttcca
     9841 gcttgtaaaa ttcttcccgt gtgatagagg tccaaagcga accgaaacgc acatccagaa
     9901 tatcaatggc tcccttcacc agatgatctt ctatgatggt cgctacgact ggaagctcta
     9961 caatctgttc cacactgagc tctggcccta cttcctcaaa agtaatgtga ccactggcca
    10021 gtttagcacc agtataggca tagacatcac gaccgtggaa ggtataagaa tgctctgtgt
    10081 tttgacgcct attggccacc tcagaaatct cacgaatggc tacaatgcca acgtgtttct
    10141 tgataaagga aagcgtccca ttatctggcg tgacaatgta ttgatttttt gcagtcttgg
    10201 caactacact cttacgtttc gaaccgacac ctggatcgac aaccgataca aacgtcgttc
    10261 cctcaggcca gtaatccacc gtctgaaaga gacgatagct cccctcgaaa atattataag
    10321 gcgtgatatc gtgcgtcaag tgatggattt ttaaggttgg agactcttct aaagccactc
    10381 caatcatagc cgataccgca ccatcaacca aaccaaagtc tgattgtaat accagtaaat
    10441 tattattcat tttatctcct tgtatatcct ttatcatacc atatttcaga gaaagtcgct
    10501 aatagctatt tcaattggtt agggtatagt tttaccttat agcaaatttc ctactaaaag
    10561 ttcttgttat tagctagtag gtgcattttt tcttgaaaaa gggtatgata gttacatcat
    10621 gaaaaagtag gttttattat gaaaattatc cttgtcggag ggggaaaagt tggttttgcc
    10681 ctctgtcgct ccttggttgc agaaaagcat gatgttttgc tgattgagca agacgaagct
    10741 gttctcaatc atattgtcag tcgctttgat atcattggta tccttggtaa cggggccgat
    10801 tttgccattc ttgagcaagc cagcgtccaa gattgtgata tctttatcgc cctgactgag
    10861 cacgatgaag tcaacatgat tgcagcagtt ctagccaaga aaatgggagc taaagaaact
    10921 atcgttcggg tgcggaaccc tgaatattct aactcttatt tcaaggaaaa aaatattctc
    10981 ggtttttctc ttatcgttaa tcctgagctc ttggctgccc gcgctatcgc gaatatcatt
    11041 gacttcccca acgccctgtc tgtcgaacgc tttgctggtg gacgcgttag cctcatggaa
    11101 tttgtcgtca agtccaccag cggtctttgc caaatgccca tttctgattt tcgtaaaaaa
    11161 tttggtaatg tcattgtctg tgcgatagag agggatcatc aaattatcat tccaagcggt
    11221 gacatgactg tacaggataa agatagaatc tttgtcactg gtaaccgtgt cgatatgata
    11281 ctcttccata attattttaa atcacgcgcc gtgaagagcc ttctcatcgt tggggcaggt
    11341 agaattgcct attatctact tggtattctc aaagatagtc gtatcgatac aaaagtcatt
    11401 gaaatcaatc ctgaaatcgc cagcttcttt agcgagaaat tcccaaatct ctacatcgtt
    11461 caaggagatg gaaccgcaaa agatatcctg ctggaagaaa gtgctcaaca ctatgatgcc
    11521 gttgcgactc taacaggagt cgatgaggaa aatctgatta cttcgatgtt ccttgacagg
    11581 gtaggtgtac agaaaaatat taccaaggtc aatcgtacca gtctcctcga aattatcaat
    11641 gcgcctgatt tttcaagtat catcacacct aaaagtattg ccgtggacac gattatgcac
    11701 tttattcgtg gtcgtgttaa tgcccagtat tcagaccttc aggccatgca ccatctagcc
    11761 aatggtcaaa tcgaaaccct gcaattccat atcaaggaag ccaataaaat gactgccaaa
    11821 cctctttctc aactgaaatt aaaaaaaggt gttcttattg cagccatcat tcgaaagggc
    11881 aagactattt tccctactgg ggaggatatg ttggaagtcg gagacaagct cctagtaaca
    11941 accttgttgc caaacatcac caagatttat gacttgatcg cgaggtaaga aatgaataaa
    12001 agtatgattc gttacctcct ttcaaaatta cttttgattg aagctgttct tcttttggtt
    12061 cctgttgcga ttgctgtcta ttaccgtgaa tcgagccaag tctttacagc cctcttttcg
    12121 accataggga ttctcgtatt gttaggcggt tcagggagtt tacagaagcc aaaaaatcaa
    12181 cggatttatg ccaaggaggg agtcttgatc gttgccctct gttggatcct ttggtctttc
    12241 tttggtggtc tcccctttgt cttttctggg caaatcccta gccttattga tgcctttttt
    12301 gaaattagtt ctgggtttac aactactgga gcaagtattt tgaacgacgt ttcggttctc
    12361 agccgttccc tcctcttctg gcgaagtttt acccacttga ttggagggat gggagtgctt
    12421 gtttttgcac ttgctattat ggacaatgcc aaaaatagcc acctagaggt gatgaaggct
    12481 gaggttccag gtcctgtttt tggcaaggtt gtatccaaac taaaaaacac tgcccagatt
    12541 ctctatctcc tttatctagc tctcttctcc ctctttgtca tcatctatta tctagctggt
    12601 atgcctctct acgatagttt tgtcattgct atggggacag cgggaactgg aggctttacc
    12661 gtctataacg acggaattgc ccactatggc agctcactga ttacctatct ggtcagtatc
    12721 ggagttctgg tttttggggt aaatttcaac ctctactact acctcatgct ccgtcgcatc
    12781 aaggccttct ttggtgacga agaacttcgg gcttacttgg tcattgtact ggtttctaca
    12841 ggcttgatta gcctcaacac cctctacctc tacccagggt tttcaaagag ctttgaaatg
    12901 accttcttcc aggtttccaa catcattaca acaactggtt ttggatatgg agatattacc
    12961 aactggcccc tcttctccca gtttatcctc cttttcctca tggcaatcgg tggctctgct
    13021 ggatcaactg caggtggact caagattatt cgaggcctca tcctttcaaa aattgccaaa
    13081 aaccaaattt tgtcaatcct atcgccccac cgtgttttga ctctccatgt gaatcaaacg
    13141 gtgattgata aagataccca gcataagatt ctcaagtact ttgtcatcta tgctatgatt
    13201 ttgctagcgc ttatctttat tgtcagccta gatagcaatg attttctggt cgtgaccagc
    13261 gctgtcttta gctgtttcaa taatatcggg cctattctag gaaccacttc tagtttctca
    13321 atctttagtc ccatctcaaa aattctcctc tcctttgcaa tgattgcagg gcgcttggag
    13381 atttacccaa tcctccttct ctttatgaaa agaacttggt ctaagagata aaatacaaaa
    13441 tccctctttt tacaattgtg aaagagggat ttttatatta aaaaggggga acgtttttaa
    13501 atccaagcct gtctgtaagc aaagttctta ctcaaataaa agatggaaaa acttactaca
    13561 cagcttggta atgctccaat acaaattgta aggctttact tggattcttt gttaaatgaa
    13621 tatattccat tcctctggta gcaactatct gaatacctaa tctttctgca tccaccttca
    13681 tctctctata ataactccga acttgtggag ccagaataat taaatcataa acacccataa
    13741 tatcataatg agctccgtac gctcctgaat tcgcaatcac tctaacttct gctaattgag
    13801 cgccctcgtt aattgcattg gctaattacg cacttgttcc agaccctgca caaagaacca
    13861 gtactttcaa ctccttactt tttattacat ctatctcacc aggcattatt tcacttgtat
    13921 cctcctctaa aataatatca tttgagcttg caatatctac aacctgtagt agatatctcg
    13981 tatttagaca atatgaaaac aagacgactt ccatatagga aaccgccttc tctctatgtt
    14041 gagtgattta tattaaaata acttttcttc tagctgcatt ttattattat aaaaacattc
    14101 atcataaccc tgaacttaaa taacgatttt tat
//
LOCUS       DAVMGZ010000041        13894 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000041 DAVMGZ010000000
VERSION     DAVMGZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.042"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            178..492
                     /locus_tag="VYQ93_001014"
     CDS             178..492
                     /locus_tag="VYQ93_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV3335716.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            508..894
                     /locus_tag="VYQ93_001015"
     CDS             508..894
                     /locus_tag="VYQ93_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV3335717.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            923..2308
                     /locus_tag="VYQ93_001016"
     CDS             923..2308
                     /locus_tag="VYQ93_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV3335718.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2311..2463
                     /locus_tag="VYQ93_001017"
     CDS             2311..2463
                     /locus_tag="VYQ93_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV3335719.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2486..3691
                     /locus_tag="VYQ93_001018"
     CDS             2486..3691
                     /locus_tag="VYQ93_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEV3335720.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            3734..3955
                     /locus_tag="VYQ93_001019"
     CDS             3734..3955
                     /locus_tag="VYQ93_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335721.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4072..4569
                     /locus_tag="VYQ93_001020"
     CDS             4072..4569
                     /locus_tag="VYQ93_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEV3335722.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            4544..5050
                     /locus_tag="VYQ93_001021"
     CDS             4544..5050
                     /locus_tag="VYQ93_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV3335723.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5034..7481
                     /locus_tag="VYQ93_001022"
     CDS             5034..7481
                     /locus_tag="VYQ93_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV3335724.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            7484..9661
                     /locus_tag="VYQ93_001023"
     CDS             7484..9661
                     /locus_tag="VYQ93_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV3335725.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERTSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            9658..10659
                     /locus_tag="VYQ93_001024"
     CDS             9658..10659
                     /locus_tag="VYQ93_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEV3335726.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            10656..11591
                     /locus_tag="VYQ93_001025"
     CDS             10656..11591
                     /locus_tag="VYQ93_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV3335727.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            11836..11952
                     /locus_tag="VYQ93_001026"
     CDS             11836..11952
                     /locus_tag="VYQ93_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEV3335728.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            11968..13887
                     /gene="tet(M)"
                     /locus_tag="VYQ93_001027"
     CDS             11968..13887
                     /gene="tet(M)"
                     /locus_tag="VYQ93_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEV3335729.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKINIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         4459 a         2548 c         2948 g         3939 t
ORIGIN      
        1 aaatttgaat atttaatagg tagacacttc aaaaaatgag gtgtctattt ttttacccga
       61 ttttgaaagg aagtgaactt atgaaaacaa aaaatcaaga atcaaaaggt cgttccccac
      121 tctttaagac catcaaacat tcattcagcc aataaaaaag aaaggatagg taaaaatatg
      181 gaacttaaat ttgtgattcc caacatggaa aaaacattcg gcaatttaga atttgctggc
      241 gaggataaag tcgttcagcg aagaatcaac ggacggctaa ctgtcttatc aagaagctat
      301 aatctctatt ctgatgttca aagagcagat gatattgtgg tggtgcttcc tgctgaagct
      361 ggcgaaaaac atttcggctt tgaggaacgt gtgaagttag tcaatccacg tattaccgca
      421 gagggctaca aaatcggcac tcgtggtttt acaaattacc ttttacatgc tgacgacatg
      481 ataaaagaat aaagaaagag aggaaaaatg atgagattag caaatggcat tgtattagat
      541 aaagacacga cttttggaga attgaaattc tctgctctac gtcgtgaagt gagaatccaa
      601 aatgaagacg ggtcggtttc agatgaaatc aaggaacgta cctatgactt aaaatccaaa
      661 ggacaaggac gcatgattca agtaagtatt cctgccagcg tgcctttgaa agagtttgat
      721 tataacgcac gggtggaact tatcaatccc attgcggaca ccgttgctac tgccacctat
      781 caaggagcag atgttgactg gtatatcaag gcagacgata ttgtgctgac aaaggattct
      841 agttcattca aagctcaacc acaagcaaag aaagaaccga cacaagacaa atagtcgcta
      901 ggtagaaagg agactttttc gcatgaaaca gcgtggtaaa aggattcgcc catctggtaa
      961 agatttagtc tttcatttta cgatagcgtc actcctgcct gttttcctgc tggttgtcgg
     1021 actgtttcat gtgaagacaa tccagcagat caactggcag gattttaacc tatcacaagc
     1081 agataagatt gacattccct atttaattat cagtttcagt gtcgcaattc ttatctgctt
     1141 gctggtagcg tttgtattca aacgggttcg ctatgatacg gttaaacaac tttaccaccg
     1201 tcaaaaactg gcaaagatga tacttgaaaa caagtggtat gaatctgaac aggtcaaaac
     1261 agagggtttc tttaaagata gtgctggtcg tacaaaggaa aagataacct acttccctaa
     1321 aatgtattat cgacttaaaa atggcttgat acagatacgg gtggaaatca cgctgggaaa
     1381 atatcaagac caactcttac acttggaaaa gaaattagag agtggcttgt actgtgagct
     1441 gacggataaa gagttaaagg attcctatgt ggaatatact ttgctctatg acaccatagc
     1501 cagtcgtatt tctattgatg aagtagaagc taaagatggt aaacttcgct taatgaaaaa
     1561 cgtatggtgg gaatatgata agctccctca tatgttgatt gctggtggta caggtggcgg
     1621 taaaacttac tttatactga cactgattga agccttgctt catacagatt caaaactgta
     1681 tattcttgac ccgaaaaatg ctgaccttgc ggacttaggt tctgtgatgg caaatgtcta
     1741 ctatagaaaa gaagacttgc tttcttgcat tgaaacattc tatgaagaaa tgatgaaacg
     1801 tagtgaggaa atgaagcaga tgaagaacta taagactggc aaaaattatg cttacttagg
     1861 tctcccggca cacttcttaa tctttgatga atacgtcgct ttcatggaaa tgctgggaac
     1921 aaaagaaaac accgcagtta tgaataagct gaaacagatt gtcatgttag gtcgtcaagc
     1981 tggcttcttt ctaatactgg cttgtcaacg tccagacgca aaatatttag gcgacggaat
     2041 ccgtgatcag tttaatttca gagtggcttt aggtcgtatg tctgaaatgg gctatggcat
     2101 gatgtttggc agtgacgtac aaaaggattt cttcttaaag cgaatcaaag gtcgtggcta
     2161 tgttgatgta ggaacaagtg tcatatcaga gttttatact ccccttgtac caaaaggata
     2221 tgatttcttg gaggaaatta aaaagttatc caacagcaga cagtccacgc aggcgacgtg
     2281 cgaagcggaa gtcgcaggtg tggactgatc ttgctggctg gtgtggcaat agccacgcca
     2341 gcacttaacc ccccgtatct aacagggggg tacaaatcga caggaaacag tcaaaaaaac
     2401 attagaaaat cctttggtta caagggattt acaaaatttc agcgtatgtc aaatgggctt
     2461 taaaagttga catacgcctt tttgattgga gggattttta ctgaatgaac aaacttggtt
     2521 acagcattta aaagaaaaac gcttggctta tggactatct caaaaccgtt tagctgttgc
     2581 gactggtatt acaaggcagt atctaagcga tattgaaaca ggaaaagtca agccatcaga
     2641 ggatttacag cagtcccttt gggaagctct ggaacgcttc aatcccgacg ctccccttga
     2701 aatgctgttt gattatgtaa ggattcgctt tccgacaaca gacgtacagc aggtggtcga
     2761 aaacatctta caactgaaac tgtcctattt tcttcatgag gactatggtt tctattctta
     2821 ttcagagcat tatgctttag gcgacatatt cgtcctttgc tcccatgaac tggacaaagg
     2881 agttctggtg gaattgaaag gtcgtgggtg cagacaattt gaaagctatc ttctggcaca
     2941 acaaagaagc tggtatgagt tctttatgga cgttttggtg gctggcggtg tgatgaaacg
     3001 ccttgacctt gccattaacg ataagacagg gattttgaat atccctgtac tcactgaaaa
     3061 gtgccaacag gaagaatgta tctccgtctt ccgcagtttt aaaagctatc gcagtggcga
     3121 actggtacgc aaagaggaaa aggaatgtat gggaaacacc ctctatatcg gttcattaca
     3181 aagtgaagtt tatttctgta tctatgaaaa ggactacgag cagtacaaga aaaatgatat
     3241 tcccattgaa gacgcagaag taaaaaaccg ttttgagatt cgattgaaaa atgagcgtgc
     3301 ctattatgca gtccgtgatt tactcgtcta tgacaatcca gagcataccg cctttaaaat
     3361 tatcaatcgg tatatccgtt ttgtagataa agacgattcc aaacctcgtt ctgattggaa
     3421 actgaatgaa gaatgggctt ggtttattgg gaacaatcgt gaacgattaa aactaaccac
     3481 aaaaccagag ccttactcct tccaaaggac gctgaactgg ctatctcatc aagttgcccc
     3541 gaccttaaag gttgcgatta aacttgatga aatcaaccag acgcaggttg taaaagacat
     3601 tctcgaccat gcgaaactga cagaccgaca caagcagatt ttgaagcaac agtcagtaaa
     3661 agaacaggac gtgataacaa caaaaaaata actcaaatac aaattcattg aatatagaga
     3721 ggagaacatt tttatgaatt ttggacaaaa cctttataac tggtttctat caaacgctca
     3781 atcactggtg cttttagcaa tcgttgtgat tggcttgtat cttggcttca agcgtgagtt
     3841 tagcaaactg attggctttt taattattgc gattattgcg gttggcttag tcttcaacgc
     3901 tgctggagta aaagacattt tactagagct attcaatcgc attattggtg cttaaataaa
     3961 accgttcttt tgtggaatat aagtggtttt cttatgttcc gcaaaggaat ggtacaccaa
     4021 acgaagtgcg gtagggattt ttgaatctct acaaagaaag gacgtgaata tatggacgat
     4081 atgcaagtct atattgcgaa tttaggcaaa tacaatgagg gcgaattggt cggtgcgtgg
     4141 tttacctttc ccattgactt tgaggaagtc aaagagaaaa tcggcttgaa tgatgaatat
     4201 gaggaatacg ccattcatga ctacgagtta ccctttacgg ttgacgaata cacttccatt
     4261 ggcgaactca atcgactatg ggaaatggta tcggaattac ccgaagaatt acaatcggag
     4321 ctatctgctc tgctcactca tttttcaagc attgaagaac taagcgaaca tcaagaggat
     4381 attatcattc attccgattg tgatgatatg tatgacgtgg cacgctacta cattgaagaa
     4441 acgggtgctt taggcgaagt accagctagt cttcaaaact atattgatta tcaagcctat
     4501 ggtcgggatt tagacctttc aggaacgttt atctcaacca atcatgggat ttttgaaatc
     4561 gtctattaaa tctgtcggta cattactact ggcagatttt ctattttacg gggtggctca
     4621 atcagctacc cctatttttt atgaaaggat tgattacatg aagaaaatac gaagctatac
     4681 cagtatctgg tctgtggaaa aggtactgta ttctatcaat gattttagac ttccgtttcc
     4741 cataaccttt acgcaaatga catggtttgt cgtgtcactc tttgcagtga tgatacttgg
     4801 caacttgccc cctctttcca tgatagaggg agcatttctc aaatactttg ggattcctgt
     4861 ggctttcaca tggtttatgt ctacaaaaac ttttgatggt aaaaagcctt atggattttt
     4921 gaagtctgtc attgcttatg cactgcgacc aaagctgacc tatgcaggaa aaaaagtaac
     4981 gcttggcaga aaccagccac aagaagccat tacagcagtt aggagtgaat tttatggcat
     5041 atccaattaa atacattgaa aacaatctcg tctggaataa agacggggaa tgttatgctt
     5101 actatgagct tgttccttac aattactcat ttctaagtcc agaacagaaa atacaagtgc
     5161 atgattcttt cagacagctt atcgcacaaa atcgtgatgg caaaattcat gctttacaaa
     5221 tcagtacaga atccagcata cgttctgcac aagagcgttc caaaaatgaa gtcactggca
     5281 agctcaaagc ggttgcctat gacaaaatcg accaacagac agacgcttta atatccatga
     5341 ttggcgaaaa tcaagtgaac taccgtttct ttatcggctt taagttgctt ctcaacgatc
     5401 aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa tgctttgtct gattttgtct
     5461 atgatgtgaa ccataagctg atgggcgatt ttgttagtat gagtaatgat gaaatcctgc
     5521 gttttcagaa gatggaaaag ctcttagaaa ataaaatctc tcgtcgtttc aaaatccgca
     5581 ggttagataa ggacgacttc ggctatctga ttgaacacct ttacggacag acaggcactg
     5641 cctatgaaga gtatgagtac catctatcaa agaaaaagct ggataatgaa acgctgatta
     5701 aatactatga cttgattaag cctactcgct gtttggtgga agaaaaacag cgatatttga
     5761 aaatccagca ggaagatgaa accgtctatg tagcttactt taccattaac agcattgtcg
     5821 gagaactgga cttcccgtcc tctgaaatct tctactacca gcaacagcaa tttacattcc
     5881 cgattgatac gtcaatgaat gtggaaattg tagcgaatcg taaagcccta tctactgtcc
     5941 gcaataaaaa gaaagaactg aaagacttgg ataaccacgc ttggcaaagt gataatgaaa
     6001 ccagctccaa tgtggcggaa gctctggaaa gtgtgaatga gctggaaacc aatttagacc
     6061 aaagcaagga atctatgtac aagctgtctt atgtggtaag ggtatcagca aatgatcttg
     6121 acgaactcaa acgtcgttgt aatgaagtga aagattttta tgacgattta agcgtaaaac
     6181 tggtacgacc atttggggat atgctcggct tacatgaaga atttttacct gccagcaagc
     6241 gttatatgaa tgattatatt caatacgtga cctctgattt cctcgctggt ttaggttttg
     6301 gtgctactca aatgctgggg gaaaatgagg ggatttatgt tggctacagc ttagatactg
     6361 gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg ggttaagggt tcagtaacca
     6421 atgcgttagc gtcggctttt gttggttcgc tgggtggtgg taaatccttt gcgaataacc
     6481 ttatcgtcta ttatgcggtg ctttatgggg cacaagcagt gattgtagac ccaaaagcag
     6541 aacgtggcag atggaaagaa accttgccag agatttccca tgaaatcaat atcgtcactc
     6601 tgacttctga tgagaaaaac aaaggcttac ttgaccctta tgtgattatg aaaaatccca
     6661 aagattctga atcactggct attgatattc tgacattcct tacggggatt tcctctcgtg
     6721 atggggaacg cttcccaatc cttagaaaag ccattcgtgc agtaaccaat agtgaagtac
     6781 gagggttgat gaaagtgatt gaggaattac gggttgagaa tacgccacta agtaccagta
     6841 tagccgacca tatcgaaagt tttacagact atgactttgc acatttatta ttcagtaatg
     6901 gttatgtgga gcagtctatc agcttagaaa aacaactgaa cattatacag gttgcggact
     6961 tggtacttcc cgacaaggaa acttcctttg aggaatatac cactatggag cttttatccg
     7021 ttgctatgct gattgtcatt agtacctttg ctttagactt tatccataca gaccgaagca
     7081 ttttcaagat tgtagattta gacgaagcat ggagcttttt acaggtagca caaggaaaaa
     7141 cactatctat gaagctggtt cgggctggtc gtgctatgaa cgctggggta tatttcgtga
     7201 cccaaaatac agacgacctc ttagatgaaa aactgaaaaa taacctcggc ttaaaatttg
     7261 catttcgttc cactgacctt aacgagatta aaaagacctt agcctttttt ggtgtagacc
     7321 cagaggacga aaacaatcag aagcgattgc gtgatttgga aaacgggcaa tgccttatca
     7381 gtgatttata tggtcgtgtc ggtgtgatac agttccaccc tgtatttgaa gaactgctcc
     7441 atgcctttga taccagacca cctgtgcgaa aagaggtgta aatgtgaaac catcaatagt
     7501 aaacagaata aaatcaaact ggacgctgaa acgtctaggt aaagtggcaa tgacagtggc
     7561 tttcacactt gtgattgcca tttttctttt agccatgctg ggaacggtgg ttcaagctgc
     7621 gggcttggta gatgatacgg tcaatgtggc aaatgaatac agccgatacc cacttgaaaa
     7681 ctatcaactg gatttttatg tggataatag ctggggctgg cttccgtgga actggtcgga
     7741 cgggattgga aaacaggtca tgtatggact atatgccatt accaatttta tttggacaat
     7801 cagtttgtat gtttccaatg cgacaggtta cttagtacag gaagcctatt ccttagactt
     7861 catttccgct acagcagatt ccattggtaa gaatatgcag accttagctg gtgtgagtgc
     7921 aaacggattt tcaacagagg gtttctatgt tggattcctc ttactcttga ttttggttct
     7981 tggggtttat gttgcctata cgggactgat aaagagagaa accacaaagg caattcatgc
     8041 cattatgaat tttgtgctgg tgtttatcct atcggcttcc tttattgcct acgctcccga
     8101 ctacattaaa aaaatcaatg acttttcatc agacatcagt aatgccagtt tatcacttgg
     8161 cacgaagatt gtcatgcccc attccgatag tcaaggcaag gacagcgtgg acttaatcag
     8221 agatagcctg ttttccatac aggttcagca accgtggcta ctgcttcaat acaacagttc
     8281 agacattgaa agtatcggta ttgaccgtgt ggaaagcctg ctctccacca gcccagattc
     8341 caacaatggc gaagacagag aaaaaattgt tgcggaagaa attgaagaca gaagcaatac
     8401 caatctaacc attacaaaga ccattaaccg tttaggtaca gtcttcttcc tatttgtctt
     8461 caatattggg atttccatat ttgtattcct attaacagga atcatgattt tctcgcaggt
     8521 actttttatc atctatgcta tgtttctgcc tgtgagcttt attttaagca tgattccatc
     8581 atttgatggt atgtcaaaac gagccataac aaagctcttt aataccattt tgacacgagc
     8641 tggaatcaca ttgattatta cgacagcatt tagtatttca accatgctct ataccttatc
     8701 ggctggttat ccgttctttt tgattgcttt tctacagatt gtgacctttg caggaatcta
     8761 cttcaagctg ggcgatttaa tgagtatgtt ttctctacag agtaacgatt ctcaaagtgt
     8821 gggaagtcgt gtgatgagaa aacctcgtat gcttatgcac gctcacatgc accgtctaca
     8881 gcggaaactt ggacgttcca tgactactct aggggctggg tctgccattg ttacaggtaa
     8941 aaaaggacag tcgggttcgg ggagttctgc aaggacacaa gcagatcact cccgaccaga
     9001 cggaaaggaa aaatcaacac ttggaaaacg tatcggtcaa accatcggta cagtagctga
     9061 taccaaagac agaatggtag acactgctag tggtttgaaa gaacaggtta aagatttgcc
     9121 gaccaatgca agatatgcag tatatcaagg aaaatccaaa gtaaaagaga atgtccgtga
     9181 tttaaccagt agtatttctc aaaccaaagc ggacagagcc agtggacgca aggaacagca
     9241 ggaacaaagg cgaaaaacca ttgcgaagcg tcgctctgaa atggaacagg tcaaacagaa
     9301 aaaacagcct gcttcttctg ttcatgaaag accgactaca agacaagaac aatatcatga
     9361 tgaacagacc tcaaaacagt ctaatattca gacttcatat aaggaatctc aacaagccaa
     9421 acaagagcgt ccagcagtta agtccgattt ttcaagtcca aaagtggaac gccaaggcaa
     9481 taccgttcaa gaaaaaaccg ttcaaaagcc agcaacttca accactacag cagatagaac
     9541 ttcacaacgt ccaatcacaa aagaacgtac gtctactgtt caaagagtac cactacaaaa
     9601 tacaagaagt agaccaccaa tcaaaaccgc caccattaag aaagtcggta agaaaccatg
     9661 aagttgaaaa ctttagtgat tggtggttct ggattattct tgatggtctt ctcactgctt
     9721 ctgtttgttg ccattttatt ttcagatgaa caggacagcg gaatttccaa tattcattat
     9781 ggaggtgtga atgtttccgc agaagtgctg gctcataagc ctatggtaga aaaatatgcc
     9841 aaagaatatg gcgttgaaga atatgtcaac atacttcttg cgattataca ggtggaatcg
     9901 ggcggtactg cggaagatgt tatgcagtcc tcggaatccc tcggtcttcc acctaattca
     9961 ttgagtacag aagaatccat taagcaaggt gtgaagtatt tcagtgaatt attagccagt
    10021 agcgaaaggc tcagtgtaga tttagaatcg gttatccagt cctacaatta tggtggtggt
    10081 ttcttagggt atgtggctaa tcgtggaaat aaatatacct ttgaactggc tcaaagtttc
    10141 tcaaaagagt attcaggtgg cgaaaaagtg tcttacccca atcccatagc catacctatc
    10201 aatgggggct ggcgatacaa ctatggcaat atgttttatg tgcaactggt aacgcagtat
    10261 cttgtcacaa cagagtttga tgatgatacg gtacaagcca tcatggacga agcactgaaa
    10321 tatgagggct ggcgatacgt ttacggtgga gcttccccga ctacttcttt tgattgtagc
    10381 ggactgacac aatggacgta tggaaaagct ggaattaact taccacgaac cgcacaacag
    10441 caatatgatg tgacccagca tatcccacta tcggaagcac aagctggcga tttggttttc
    10501 tttcattcta cctataacgc tggctcttat attactcatg ttgggatata ccttggcaat
    10561 aaccgtatgt ttcatgcagg cgacccaatc ggttatgccg acttaacaag cccctactgg
    10621 caacagcatt tagtgggagc aggacgaatc aaacaatgag aaaggaagat ttaatgatga
    10681 aatttagaaa aaatcagaat aaagaaaaac agataccaaa ggaaaagaaa cctcgtgtct
    10741 actataaggt caatcctcat aaaaaggttg tgattgcctt gtgggtactt ttagggctta
    10801 gtttcagctt tgcgatattc aagcacttta cagctataga tactcatact attcacgaaa
    10861 caactatcat agaaaaggaa tacgttgata ctcatcatgt agaaaatttt gtagagaact
    10921 ttgcgaaagt ctactattca tgggagcaat ccgataagtc cattgataat cgaatggaaa
    10981 gtctaaaagg ctatctgaca gatgaacttc aagctctcaa tgttgataca gtacgcaaag
    11041 atattcctgt atcgtcttct gtaagaggat ttcagatatg gacggtagag ccaactggcg
    11101 acaatgagtt taatgtaacc tacagtgtag accagctcat tacagaggga gaaaatacaa
    11161 agaccgtcca ctctgcttat atagtgagtg tctatgtaga tggttctgga aatatggtac
    11221 tggttaagaa tccgaccatt accaacatac ctaagaaatc aagttataaa ccaaaagcca
    11281 ttgaaagtga ggggacggtt gattccatta caaccaatga aatcaatgag tttttaacga
    11341 cgttcttcaa gctctatcct acagcgacag ccagtgaact ttcctactat gtgaatgacg
    11401 ggatattaaa accaatcgga aaagagtaca tctttcaaga actggtaaat cctattcaca
    11461 atcgtaagga taatcaagtc acggtatcgc tgacagtgga gtatatcgac cagcagacca
    11521 aagcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    11581 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    11641 gataaaaaat tggagattcc tttacaaata tgctcttatg tgctattatt taagtatcta
    11701 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    11761 tggaaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    11821 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    11881 atggttatgc ataaaaatcc cagtgataag agtatttatc actgggattt ttatgccctt
    11941 ttgggctttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    12001 gttgatgcgg gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    12061 gaattaggaa gcgtggacag aggtacaacg aaaacggata atacgctttt agaacgtcag
    12121 agaggaatta caattcagac ggcgataacc tcttttcagt ggaaaaatac taagataaac
    12181 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    12241 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    12301 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    12361 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    12421 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    12481 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    12541 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    12601 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    12661 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    12721 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    12781 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    12841 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    12901 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    12961 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    13021 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    13081 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    13141 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    13201 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    13261 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    13321 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    13381 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
    13441 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    13501 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    13561 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    13621 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    13681 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    13741 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    13801 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    13861 cgatatatgt tcaataaaat aacttagtgt attt
//
LOCUS       DAVMGZ010000042        13280 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000042 DAVMGZ010000000
VERSION     DAVMGZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.043"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            133..309
                     /locus_tag="VYQ93_001028"
                     /pseudo
     CDS             133..309
                     /locus_tag="VYQ93_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            650..1114
                     /locus_tag="VYQ93_001029"
     CDS             650..1114
                     /locus_tag="VYQ93_001029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3335730.1"
                     /translation="MRRKYKSIALKKELANDSGKKKCHAMKAQAIVTSQGRIVSLDIT
                     VNYCHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQIPRKSSKLKPLTVEDKTY
                     NHALSKDRSKVENILAKVKTFKMFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            complement(1184..1546)
                     /locus_tag="VYQ93_001030"
     CDS             complement(1184..1546)
                     /locus_tag="VYQ93_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335731.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSLEQYPDYDGFDYEEW"
     gene            complement(1924..3804)
                     /locus_tag="VYQ93_001031"
     CDS             complement(1924..3804)
                     /locus_tag="VYQ93_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV3335732.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIVGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGSETAQFSILSSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3798..4667)
                     /locus_tag="VYQ93_001032"
     CDS             complement(3798..4667)
                     /locus_tag="VYQ93_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV3335733.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQTCTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4751..7396)
                     /locus_tag="VYQ93_001033"
     CDS             complement(4751..7396)
                     /locus_tag="VYQ93_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV3335734.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKDHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLSRQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7487..8767)
                     /locus_tag="VYQ93_001034"
     CDS             complement(7487..8767)
                     /locus_tag="VYQ93_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV3335735.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEKNWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSESIIADTKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8938..11022
                     /locus_tag="VYQ93_001035"
     CDS             8938..11022
                     /locus_tag="VYQ93_001035"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV3335736.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTGQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKSSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQERGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLNKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(11062..12741)
                     /locus_tag="VYQ93_001036"
     CDS             complement(11062..12741)
                     /locus_tag="VYQ93_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV3335737.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGE
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPAYG
                     NGGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELDYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GSALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFSRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGEALHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3713 a         3134 c         2695 g         3738 t
ORIGIN      
        1 gtgttaaaaa agaaattaaa gtgggttaaa aaagaaatta actgagtaaa aactctcgaa
       61 attcaattcg ctatattatc actcaacttg aacaaatacc agaaccacaa aaaacagatg
      121 taaaggaaaa atatggactt aaaatttgaa ggggttgatt tagaatacaa aaaagctaaa
      181 aacaatcttc cagagtcttt ctgggaaacc tactcagcat ttgcaaatac aaatggcggg
      241 aaaataattc tcgggataga tgaaaaaaac attgacacct accaaagagt caatagactc
      301 cctgcgaaac aaaattatga agcaagtaaa caactaactg atgcacgatt taagctcctt
      361 gttggtgttc agcgtaccac ttttgaagag atgttagctg tattaaaaac agcttatcaa
      421 cttaaacacg caaaaggtgg acgaaaacct aaattaagcc tagaagacct tcttatggcc
      481 actcttcaat atgtgcgaga atatcgaact tatgaagaaa ttgcggctga tcttggtatt
      541 cacgaaagca acttaatccg tcggagccaa tgggttgaag taactcttgt tcaaagtggt
      601 gttacgattt caagaactcc tctcagctct gaggacacgg taatgattga tgcgacggaa
      661 gtacaaatca atcgccctaa aaaaagaatt agcgaatgat tctggtaaaa agaaatgcca
      721 cgctatgaag gctcaagcga ttgtcacaag tcaagggaga attgtttctt tggatatcac
      781 tgtgaactat tgtcatgata tgaagttgtt caaaatgagt cgcagaaata tcggacaagc
      841 tggtaaaatc ttggctgaca gtggttatca agggctcatg aagatatatc ctcaagcaca
      901 aattccacgt aaatccagca aactcaagcc actaacagtt gaagataaaa cctataacca
      961 tgcactatct aaggatagaa gcaaggttga gaacatcctt gccaaagtaa aaacgtttaa
     1021 aatgttttca acaacctatc gaaatcatcg taaacgcttt ggattacgaa tgaatttgag
     1081 tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta ttgaaatact
     1141 aagcctacac agactgattc caattgtttt ttagatttca attttaccat tcttcatagt
     1201 caaaaccatc ataatctgga tattgttcta gcgacgttcc aaccgaacgt acgagataag
     1261 cttcttcgtc ctctgtctcg atttctctca gcacttggca ttcaaaacgc cattcatcac
     1321 cgaagtcgaa aataaactta aatttttgtt tgacacttag gctatccaga tagacatttt
     1381 ctgtgtaacg ttcttcaaca tcgtcactaa caaagctaag aaagtaagaa tctgcatgac
     1441 tccactcaac attatccatg aaaaatgcgt gagcatgatc atttgcaaaa tcaaaactcc
     1501 ataaaatcac atctgctagt tccgctaacg tttttgtttt tggaatctat atatgtcggt
     1561 agcaaccttt ttaaaatttg acggaaatga tccaagcttc tacagatgct ggcttttcag
     1621 cttctatttc ttgttttact aattttaagt gtagcataat acctccaatt gctcatcgat
     1681 agaattgctc ttaggaaaca ttcactttct gctattttat cataagtttg tctaactgta
     1741 gcatttgaaa ccagataatt cttctgtttc acgcgaaaca ccctgcgcca agcaaggtgt
     1801 tcttgttttt atactcaatg aaaatcaaag agcaaactag gaagctagct gcaggctgct
     1861 caaaacactg ttttgaggtt gtagatggaa gctgacgtgg tttgaagaga ttttcgaaga
     1921 gtattaagtc gtataaatcg tcgaagctgt cgcaatggta tgccactggt tagctgtagt
     1981 tgctgcgaaa tccttgtctg cgtagaagtc agtaaatggt aggatttcaa cttccagctc
     2041 gtcgatgcgg tcaagctggc cagccagata aacctcgata cggctttctg ctcgtttgat
     2101 gcgttgcaag agtccgccca tacggatgtc aactgtatcc aaaccaaaga ccttgttttc
     2161 tttcagccat tggtgactaa agagggcatg gaagtcttca atttggcttc taagttctgg
     2221 taattcttgt ctggcgattt gttgtaaact ttctttatca tccgcttggt aggcctgacg
     2281 aatgcgtcgt cccacatcta ctttgctact taaaatagca ttcaactggg cctgagtttc
     2341 aaagagataa gcatagtttc cagctttttc tttaatgtta gcaagcgtct cagcagcctg
     2401 agcgaagtgc ggtttgtcct gttcaggtgt catgtgtcgg tcaagtatcg gacaaagaac
     2461 atcctgataa aaaacatagc ggttgggatt gataccgctg agattgcctg gtaggtctgg
     2521 taagaggttg gcaaggtcaa tctgcataaa atcctcaacc gttagaccag tattggtctt
     2581 gaagtgcgca gacaaactat ctaggtcatt gcgatagctg agttctgccc agatttgcaa
     2641 gcttgatagg atagagaact gggcagtttc actaccattg tctccccaac ccgttacgat
     2701 gacttcttta atctgattgg cacggcaggc tttattagcc tcgatagcca ctagacggct
     2761 aaaatggttg tgaggtgtaa agccaatcca cttccaagct ccccctgcaa atgcaaggtc
     2821 atggctaatc ttgtgatgat tgcggaaatt acggttgtat ttttcctcgc tatcctgata
     2881 ataatcccag taaaccagag tcacacggtc tttgagacgg tctaggtaga cacgagtttc
     2941 ctctggaatt tccacatcac ggtcgtactg gccatccgct gacatgagtt tgaagaacat
     3001 atcactccac atctggcagt ggaaaccata tttgtcagca atatccagca cgcgctccaa
     3061 gtgttggcac atgaggagac tacgatccac aacaccgttc aaaatcaggt agcgtcccaa
     3121 accaaccaag tgggcttcgt ccatcccgat attgaccttg cgagtcttca gtttagacaa
     3181 cgtggcaaac atgccatcaa tcaggtcata aaccttttct tcgccaatga gaagaatgtc
     3241 ctctacatca cggagctcct gcacttcctt gacaccccat ttgacaaagg ccgacaagtg
     3301 ggccaaggtc tggatgcatg gtacaaaggt catgtcaaac tgttgagcat aggcttcgat
     3361 ttcctgcaac tcctctgctg aataagctcc acggaaatag ccaaagtaag gctgcccttc
     3421 aatctggtaa gtgtcttcca tgtaaagctc aaaggttgag tagcccatga gagccaagac
     3481 ctcaatcatc tgcttggcag aagccacatt cagcaccgca tttcgagaac agtcaaccat
     3541 gtaagccaaa tcttcgtaag ctgcttgttc ctcaatctct actttatcag cttctgctag
     3601 aactgttacc aacaaggata aggcacgata aagttggtga ggtttgcggt aggtcaattg
     3661 atagtgacct tcctcaccca cgatagagat agaggcttgg tcagactgag tgacagccac
     3721 ttccacatct ggtagagaaa tatgaccttt taaaacctca atagcttgcg tttgtttggg
     3781 actaagtcct gtaaatctta ccattgcttt tcctcctgta gccagttgac aagggcaccg
     3841 tagagattgg catctgcgtg ataggtgcag gtctggataa ctggtgcgac cgtgtattct
     3901 tcgtaggtat cgacaaagtt atcaacagcc ttcttgacat cttggataaa atctggattt
     3961 tgactgatag agccacccag actgatgaca tctggatcga tgagatactg gatattgagc
     4021 aagccttgcg ccagattgcg gttcatgcgc tcaatggctt cttgacaaag agcattacca
     4081 gctgcggcct cttggtaaat cttgcgaccg tcccaatcag tatgaccaga tttttcaatc
     4141 acgtatcgta ccatattccc agttgatgct agttgcgacc agttattaag tttttcagca
     4201 ggggcaaggg ttgtcatgta gccaaattct ccacccaagc cgtggcgacc tcgatgaagt
     4261 ctaccattga taatcatggc tccgccaatc cctgtcccaa tcacgacaca ggctgcattt
     4321 tcaagctctg gatgagctag tagttcactg agtccaacgc agttggcatc attttctaaa
     4381 tggacaggta gctgataaga gctaagcgcc tcataccaag aaaagccatg gatgtagggc
     4441 accgcactga agccatcaat cacacctgtc tcttgattga ccgcacctgg aacgctcata
     4501 gcaatcccac tgtaatcctg ttctgacaag cgttgatcta gccacgctag taaatcctcc
     4561 aagttttcag gcgttgaaat acttgtctta tccagtattt tcccatcagg agtcagactg
     4621 gcaaacttaa tcccagtccc tccgatatca atcgttgcaa tggtcattgt ttttaccata
     4681 aaaaggggcg aatcagcagt tagttcttac cttttcgccc ctcctttctt ttgatgttta
     4741 ctttttgaaa ttaaatttct tcttttttga tgaattctgt acgaatctct tgtggagcca
     4801 atagtcctga atgaactgga tatggtcgct caagtaagtc aagaatggtt tgttgatgtt
     4861 ctgatatgcg cacattttct tgactcatat tatagtaacg gaggacatag ccttcttcat
     4921 tttccgccac tttaaaggct gttggacaaa cttgcggtat gctgagaaca gaatggctca
     4981 agaggctacc agtcgcaacc acgcttcctt cctgtctaga aagctgaagg ctggtaaacg
     5041 gtgtctgcaa ggctttggca cgacgatagg ctgagaagcg ttcttgggct tggtggcatt
     5101 caatcgcaaa ttcgacttca aactcacgca agcattgtgc ttctggcgtt gggaagtagc
     5161 cccagtcacc tagctcacct gatgcacgca aaatggtcac ggcaatggtg ttatccccaa
     5221 ggatttcgta ttcattcaat cccttgttgg atacagtcac ccctttttca tcgtcataca
     5281 gactgacaaa agcttgttgg tgttgaggat tttcaggatt ttcccatgaa gcagctggtt
     5341 tgtttggtcg tgtcaccacc tcatagatac tttcagaatc attgcttgga cgcgtgttat
     5401 gagtcttgac caagagacgg atacggtgat ccttggcagt gttagtaaag cgagtcttga
     5461 agcggatttg tggattgtca acgaagacag tcaactcagt ttccagagga atgtttgtca
     5521 attcttctga ccgtccagcc tcacgcttca taaactcgat gataccttgt tgctcttctt
     5581 ctagcttttc atccgcactg acaggcacgg tcaattcatg tttgagcaag attttagcat
     5641 agcgatctgt gttttccaag acctcgtggt ccttaagctc tgcaaagatt ggctctgttc
     5701 cttttggttg gaaatagata tactcgtttc cgatgtcacc ccggtcttca aagcggataa
     5761 agtcttcata ggcttcgtga gttgtcttat catagactgt gatgttgtca tccacactca
     5821 ccgttacgaa tggtgtatca atcactccgt tttggtaaat accctcacgg tgttcttgtt
     5881 ctccttccag caattggaag gttgtccaag aaagcggtgc taggtgaact ggaatggtca
     5941 cgcgcacttg tcgagcaata cgagcttggc ggaacttgtc ttttggtaaa tcgtattcaa
     6001 aattagctcc gaggtcttcg attgtagcct ctacaggacg accatccaag tcctccacac
     6061 ggtaacttgg caagataaga gcagccatct ttttgtagcc ttctgttggg tgcaattcct
     6121 tgaaatcaca agtcgccaca tcaatcactg tgctgacagt atcgacctta tcatgcaagc
     6181 ctgtgttaat gacagtaaag agatagtcac tttgagcctt atccgtagca attttaccct
     6241 tccactcgtt gagcaaatta cttttaacaa agtttcctac ttggttgacc ttggcaaaac
     6301 gcgtttccat ctcgcggtga acttcgtcca cgctacagcc acagatacta tcatgtggcg
     6361 cattctgcaa aagtgttttc caagcatagg tcaactggtc cttgtggttg tgtccaccag
     6421 tgataatagt caagggttct accacttgct ctaggaggtt gctattttct tggaaggctt
     6481 gttttaggta aatgcgggat gaagaagtgt tggcaagtgt gtaccagcca tctgtttcct
     6541 gactggtcaa ctcacctgta acagttgata agtgttcagg aagcgcacct tctacagctt
     6601 gaacatattc atcaaaagaa ctatgaacaa agattacatc cgggaagagt tcatttgcca
     6661 cacgaatggc ttcgctcaga tttttctgga caggctggtg gtcacagccg ttcatcatca
     6721 accattggtt ggtcgaagcg taggcacgca catctgacaa tttttgtttc cagaaggtca
     6781 aggcctcatc tttgtcaact gggatttcat tcccattact gtaccagttg gcaaagagaa
     6841 tacctaaaac acgactaccg tccacaccct gccagtacat ttctgaaaac tgggatgtaa
     6901 actgctcatc ttcaaggact tggttgtcaa atccaatcgg cttcacacca cgaccaaagg
     6961 ccgccacgtg aatgcctgat ttttgaagaa tttgaggcgc ttgtcccata tttccaaagg
     7021 tatctggaaa gtagccaatc tgggttgatt taccccattt ggcagcttct tgttgaccaa
     7081 tcaaggtatt gcggacattg gcttcactgg agatcaagta gtcatcctgc aagatgtaaa
     7141 agggaccaat tttaagtttg ccctcgtcaa tgtagcgttg gaccttgtca cgattttcag
     7201 ggcgaatttg taagtagtcg tcaaggacaa tagtttgtcc atccaagtgg aaactcttga
     7261 actcagggtc attttcaaag agatcaaaga gattgtcaaa caattccacc aactgcatac
     7321 gatggctttc aaaaggcaag taccactcac ggtcccagtg actatgtgag ataatatgta
     7381 caacaacatt ttccatgaag taaaacctca ttctaaattt aaattttaac gttttaaatg
     7441 taaacttgtg attctgataa gaatcggttg agctaaagcg agccccttag cggatatcca
     7501 agtaatccaa gaccaactca cagaacatca tgttagccca ggagaaccat tcacgagagt
     7561 agagggtcgg atcatctacg tggaagcttt cgtgcatgac acctgtacca ccatcgcagg
     7621 caaccagctg atccagcaag aattttttct ctgccttatc tcttgttgtc aatccttgga
     7681 tagaaagggc gattggccag atatagcgat agaaggtatg agaacttccg agaccgctag
     7741 cgtattctcc ttggtagaag tatggatttt cagagctcaa aatggtacgg cgagtagctt
     7801 gatacacttc atcatcgacc gaacagtagc ccagataggg cgcagctagt agacttggta
     7861 catttggatc atccatgatg ctggcatttc ctaggccatc cacttcaaaa gcgtaaatct
     7921 tttcgccctt gctgttggtg gtgtaagcgt agttttcgat tccttcttgg atttcatcct
     7981 gaagacgctt ggtatcagca ataatgctct cgctatcagc taggtttaat gctgcgaaga
     8041 tttcttgcac ataacccaag actactacag caaacatatt tgacggaatc aagtaactat
     8101 actggcaaca gtcatcactc ggacgaaagg ctgaccaagt catacctgtc actgcaaagt
     8161 caggtccaaa gccatcattt accaaggtgt cttccttacg gtccgtatct cggacaaaac
     8221 gataaggaga gttcttgtgg tcttgttcaa ctgtccatag atggagaatt tccttagtcg
     8281 ctgcgacaaa agtctcatca aactgaccag tctcgccagt ctctttccag aggagataag
     8341 ccaactgcaa aggatagcaa agtgaatcca cctcatactt gcgctcccag atccagccgt
     8401 taaggtctgt gtggtcagtc tcgtggtgcc ctttccagtt cttctcaatg ttgaaggagt
     8461 tagcataggg atccttgagt accaaggtca tctgacgttt gaccaaacct gcaatggtct
     8521 gacgcaggag ggcatctctt ttagctacat gaaggtaggg tctgagttgg gctgtcgaat
     8581 ctcgaagcca catggcagga atatccccag tcaagacaaa agttgaacca tcttctaaga
     8641 tttcaaccgt attgtccaag gtatcggtgt agcaacgctc gaaaacatcc acccactctg
     8701 gatggtcctt agcccgctct gctacttcat ctagccattc tctaacaatt tctttcgaat
     8761 aaatcatcgt gcaattccct ctttcaaaca agtttcatgt tcagtatata gcttttgaga
     8821 cagaaaatac atacacaaat gatagtcatt tttctacaaa atagttattt ttgaaactcc
     8881 ttttctaaag gccctctttt tctgatataa tgtagaaaaa cagctaaagg aaagactatg
     8941 aaaccactac ttgaaaccat cgatacccgc tttggaacta ccaacaagca tgccttttct
     9001 cggggaaata ccctgccata cacaggcgtg ccttttggga tgaattactt tgtgccccag
     9061 accagtgacc aggatggatc ttggtttttt gatccgcatc tgcctatctt tcaggggatt
     9121 cgattaactc accagcccag tccttggatt ggggactact cttggctcct tctgacacct
     9181 gtcacaggtc agcttggggg agacagtctc tttcaccgcc aatcttccta tgatagggat
     9241 aaggcctctt tccaacctca ttatctaaag attttctccc tgcgctatca gattgaaacc
     9301 cagctcacac cgacttgcta cggtgcttct attcgtttga agcaaaagca aggcaaagcc
     9361 ctctccctct atcttcacgc agcagatgaa ctgacagtag agcaagtaga taagcgaact
     9421 cttgccctgc gacaagaagg taaaactgaa accaacaaaa attcgctaac gatgttcact
     9481 gccctgcaaa tgaacacgga tattcttgct atcagccaag aagctgggga ctggcgaatt
     9541 gacttagcaa gtagtcaaac cgagatgcaa ctagccactt ctttcatctc tccttctcaa
     9601 gcactgctta atctacctca agaagatttt gatagctgta aatcaagtgc ccaagcggat
     9661 tgggaaaatc tcctccatcg ttttgacatt atagagatag gagaggctga ccgaaccttc
     9721 tttgaccact gcctctacag actcttcctc ttcccacaga ctttttatga ggttgatgag
     9781 tcagggcaag ccatccacat ggatctggct actggtactg tcaagcctgg tgtcctcttt
     9841 agcaacaatg gtttctggga taccttccgc accaccttcc ccctctttgc ccttatcata
     9901 ccggagcact atcaacgctt tttagaaggt ttcctcaata gctaccgcga tactggtttc
     9961 cttccaaaat ggctggctcc agatgaacgg ggtatgatgc caggtacact tttagacggt
    10021 attatcgcag atagcgcctg caaggacatg gcccctgacc tagaaggaga actcttccaa
    10081 gccatgctcg aaacagccag caaagccgac cctctcggca tcaatggccg ccacggacta
    10141 gcccaatacc aagaacgagg ttacctctct accgaccacc acgaaagtgt tagccatact
    10201 ctagactatg cctatagtga cttttgtatc gccagctgtg ccaaaaaact agagaacata
    10261 gaaatcgctg aaacctacaa ggctgcttca caaaattacc gccagctatt tgacgctgag
    10321 acaggttaca tgcgagcacg agacaatcaa ggaaactttc accctgactt ctctccttat
    10381 agttgggggc gagactacgc tgaatgctct gccattcaag ctactttagg cgttctccac
    10441 gacatccctg gcttaatcca acttatgggt ggaaaagaaa cctttagcaa ctatcttttg
    10501 aaagcctgtc aagatgctcc cctctttgaa acaacaggct atggttacga aattcacgaa
    10561 atgagcgaga tggctactgc tccttttgga caaatagcca tttccaacca gcctagtttc
    10621 cacattcctt atctcttccg ctacagcgat taccctgact acactgccct tctcatcaag
    10681 acgctccgtc agaaagcttt tcacccaagt tgggaagcct atcctggaga tgaagacaat
    10741 ggtagtctct ctgcttggta catctggtca gctctcggat tttatccaac ctgtccagga
    10801 aaaccaagct atgacctcgg aatccctctc tttgaccatc tacgagtcta cctagctaaa
    10861 gaagataaat ggctggatat ccatactaaa caaaaccaca accattttaa ctttgtcaaa
    10921 gaatgccgac tgaacaaaac actcgtatca actattcaac accaagacct cttaaaagct
    10981 gaacaactaa ctttcaccct cagctggcta ccaagtcact aataaccaaa agaacctttg
    11041 catcgcgcaa aggttcttct tttatgaaac ttggacttga agttgatcca caagctgacc
    11101 atccaccgtt aggttcagat agaaatccaa ggttttatct cctgcgaaat acagggttgg
    11161 tagatgcaaa tcttttttgc tttcccctgc ttggaattga aggacataaa tctcgtcctg
    11221 ataggcgaca ccatggacac ctgtccccgg ttgaatcgaa atcttagcct ctaaagagga
    11281 gctactttca ctacgttcaa tcctaaaatg aagagcctct ccctttgcca cagctaggct
    11341 tttttctgca aatgctagtt cctgaacaac ttcttttttt gataatccag gagttttata
    11401 aagggaaatc ttggtcaata caggaataga ttgtgcctcg gtaatcatca cgcgcacctt
    11461 ttgtgcctct actagcggac ctcgcaagag gcgcttatga ccaactgtga agcccctgct
    11521 aaattcctgc cagacaccat ccacctctac ttgcacatga aaagcagcga ttcgttgccc
    11581 tagcttcaaa tcttctctta actcaattac atcaaaagtt ttaggagaac ctaagtcgag
    11641 ttctaactgg atgggcaagt ctgcatcgct tgcccaagag ctggtctcaa ggccgtctgt
    11701 caaatggcga caagcaaagt ctgcggaaag agctgaacca gatacctcag ctcccagagc
    11761 caaatcttct ttatagagct cattgcgata ggtcgcaaat tcataaagtc gttcaatatc
    11821 ctttgcatca aagagcccag cttgattcgg cggaatatta agcaagagtg gagttcctcg
    11881 ccctactgag tgaaagtaga tttcgaccaa ctcctcgaga gacttaggat cctgatcctc
    11941 atggtagaac cagcctggac ggatggaaac atctgcctct ccgattgaaa aaatcgtgcc
    12001 cgagggatcc ccgtgctgaa gatagtccag ctctgcttct gttcctagtt tatcaggatt
    12061 caccttttgc cacagtggat cacctgcata ccctcgttca ttgccaatcc agcggatact
    12121 ggtgccttct gttgaaaaaa tcaagcaatc gccctgcagg tcacgaatgg tttcaaacca
    12181 tttttcaaat tcataattaa ccttttgcgc gccctctcct ctggcaccat ccatccaaac
    12241 ctcagcaaat ttccctccat ttccataggc aggatttgat aagatttcct tcagctgagc
    12301 cagataatag gcattgtagt ccgcttctcg gtccacatga tagaggggac tatgggcatc
    12361 ccacggtgac aggtagaccc ccatatccat atcaaattct gtggcagctt gggatacttc
    12421 aaggagcaag tcgccctctc ctctcctcca aggactgacc ttaaccgaat aatctgtgtg
    12481 agctgtcgga taaaggacaa agccatcgtg gtgcttgacc accaaaatca actttttgaa
    12541 gcccgtttcc ttgagcacac gaacccactc acgcgcatcc aactgactcg ggttaaagcg
    12601 ctcaggatcc tcctgtccag tcccccattc ttggtcataa aaggtattag gaccgaagtg
    12661 gataaaagct gctagttcat ctcccagata agctagctga gtctgacttg gtaacggtcc
    12721 atgcggtttg attttcttca tcgttatata tctccttctt tagcaacttc tcttgttcac
    12781 aaggttatcc acaggttgtg gataataaac catgatctaa cagccagctc tttccaactc
    12841 caactggtag gctcaggatt tttctcctca atattccctc ttcaaacagc tctgtcatat
    12901 gattctgcta acacctctca gaatgctact ctccataatt attctcaggc ttctttcagg
    12961 tcattgtgtg aagaccattc actctccaga cttttccaca gattgtgaat aagtgattat
    13021 tctaagctat cacctcgtcg actataacaa aaaataaaaa gagggtcaat aagataatta
    13081 tctcttccta aatactcaga aaagattcta taatttgtac aattgaacaa gaaatgcttc
    13141 tccaaaatct atcttcaaaa gaatgttgca tctttttatc ctttcacaca aacatttctc
    13201 tttatacaga tccaagtgat aaccagatga taattggaca ctttttggtc atttgtatat
    13261 tttttgaaaa attttttaaa
//
LOCUS       DAVMGZ010000043        13252 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000043 DAVMGZ010000000
VERSION     DAVMGZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13252
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.044"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(71..706)
                     /locus_tag="VYQ93_001037"
     CDS             complement(71..706)
                     /locus_tag="VYQ93_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV3335738.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(708..1535)
                     /locus_tag="VYQ93_001038"
     CDS             complement(708..1535)
                     /locus_tag="VYQ93_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3335739.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(1557..1892)
                     /locus_tag="VYQ93_001039"
     CDS             complement(1557..1892)
                     /locus_tag="VYQ93_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEV3335740.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(1893..2424)
                     /locus_tag="VYQ93_001040"
                     /pseudo
     CDS             complement(1893..2424)
                     /locus_tag="VYQ93_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            3055..3666
                     /locus_tag="VYQ93_001041"
     CDS             3055..3666
                     /locus_tag="VYQ93_001041"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV3335741.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            3711..4439
                     /gene="rlmB"
                     /locus_tag="VYQ93_001042"
     CDS             3711..4439
                     /gene="rlmB"
                     /locus_tag="VYQ93_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV3335742.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(4478..5389)
                     /gene="trxB"
                     /locus_tag="VYQ93_001043"
     CDS             complement(4478..5389)
                     /gene="trxB"
                     /locus_tag="VYQ93_001043"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV3335743.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(5455..5679)
                     /locus_tag="VYQ93_001044"
     CDS             complement(5455..5679)
                     /locus_tag="VYQ93_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV3335744.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(5757..6500)
                     /locus_tag="VYQ93_001045"
     CDS             complement(5757..6500)
                     /locus_tag="VYQ93_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3335745.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(6500..7300)
                     /locus_tag="VYQ93_001046"
     CDS             complement(6500..7300)
                     /locus_tag="VYQ93_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3335746.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(7458..7814)
                     /locus_tag="VYQ93_001047"
     CDS             complement(7458..7814)
                     /locus_tag="VYQ93_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV3335747.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(7827..8339)
                     /locus_tag="VYQ93_001048"
     CDS             complement(7827..8339)
                     /locus_tag="VYQ93_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV3335748.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(8339..8833)
                     /locus_tag="VYQ93_001049"
     CDS             complement(8339..8833)
                     /locus_tag="VYQ93_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3335749.1"
                     /translation="MEIRLAFPNEVDAIMQVIEDAKKCLADAGSNQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            8968..9414
                     /locus_tag="VYQ93_001050"
     CDS             8968..9414
                     /locus_tag="VYQ93_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV3335750.1"
                     /translation="MKTTFSYPKWAEIPNINLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            9411..10058
                     /locus_tag="VYQ93_001051"
     CDS             9411..10058
                     /locus_tag="VYQ93_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV3335751.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(10079..10660)
                     /gene="pdxT"
                     /locus_tag="VYQ93_001052"
     CDS             complement(10079..10660)
                     /gene="pdxT"
                     /locus_tag="VYQ93_001052"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV3335752.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(10661..11536)
                     /gene="pdxS"
                     /locus_tag="VYQ93_001053"
     CDS             complement(10661..11536)
                     /gene="pdxS"
                     /locus_tag="VYQ93_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV3335753.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(11688..13067)
                     /locus_tag="VYQ93_001054"
     CDS             complement(11688..13067)
                     /locus_tag="VYQ93_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV3335754.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
BASE COUNT         3862 a         3076 c         2329 g         3985 t
ORIGIN      
        1 actttttatt ttaaaggatt ttagaatttt ttacaagata ttatgctcaa atttaacatt
       61 atttagctat ttagtaaaga tagtctttca aggcttttga caaagcctga taaccagcaa
      121 tactgaggtg cagtccatca gttgtatatt cttttttgag ttggcctgct tggtctgtca
      181 aacaatcaaa tactggcaca aattccacct gcatataggc agatgcaagc tcttgatagg
      241 cttgattcca gttctgaatt ttttcattcg agcggatata gactgcctgc tggtactcct
      301 ctccctcatt gacaggcaaa atggaaagca atttaatctc tgtcaatgga taatcgcgag
      361 caacggattg aatgatagct tcgagattat tgagagcctc attcacagga acatcctttc
      421 cgatatcatt tgtcccaatc agaagaaaaa ttttatctac tgctccacca tatagatgag
      481 catcaaggtt ctctagtaac agtcctgtct gatagccacg aattcctcga ttgacaatcg
      541 tctttgaagt cccaaatagc tcctgtagag gataatactc gacaatggaa tccccaataa
      601 aaagaatgtt tggttctaca acagaaatgt gatttaggtg acgatactta gtttgaattt
      661 tttcttgttc ctttaggagc caattttcta ataactgtac tgccacttca ttctcctttt
      721 tctaaccagt tttccaagac ttgataaact cctccttggc tgttggctgg tgctagagca
      781 gtcgccacag ctttggcttt ctcatcagca ttttccatcg cataggcaat tccagccatt
      841 tcaagcattt caacatcatt ttcactatca ccaaaagcca tgatttcttg ggatttcaag
      901 tcccagcgct tgagtaattc ctccaagccc catgctttat gaatcccagc ttggaggata
      961 tcaatgcaac catagccact ggatacagct cggacacggc catcaaagag agcattgatt
     1021 tcttccaaaa ccgaactcaa acgttcctca ccaacaacca tgctcatctt gagcacacca
     1081 ccaaagaggt cagatgttaa ttcatccaca aattgcatcc gttggtagaa tttttcaatc
     1141 atttctggag tcataaaact ttcaagatct gtaaaaatcg taccttcctt gacaaaatca
     1201 cccttcatcc ccgttacaac aaactggtcc tgacacgctc gacccttgaa atgagtcaaa
     1261 gccttgttga caatggcgtc atcccatgtc tgagcctgaa tcaattcatt gttttcaaaa
     1321 atacgagcgc cattagcaac aaccagaacc actcgatcca ccaagggact cagtagttgt
     1381 ctcatgcggt gaatttcatt gcccgtcgca atgacaaaac gaatgcccct ttgatccaac
     1441 tgatctaaaa tcttttccaa tcgtgggaga tcaagctgac ctctagcatc cagcaaggtc
     1501 ccatccatat ctgtcgcaat taccttaatc atcatttact ttcctctgga ttcaagctaa
     1561 taccacttca atcccacatg ttcattcatt tctttatagg ctgtatcaat tcgttcctta
     1621 gtcgcttcat ctaactggtc acgatgactg ccatcctcta taaaatcttc caaaatataa
     1681 gtttggtaat gatacaaggg atatccttct ggtgaaaagg ttccctttgg tgttcgattg
     1741 acccaagtag gatgagcttt agctgttccg atagttgttt ttccatcctt cttcttgatg
     1801 gtgacatcca tgagaacacc acgttcagtc cacttagcat tctcttcatc tcccatagat
     1861 tcaatccgtt gattggaaat gaagttcccc atttaataga caatgagctt cttatctcca
     1921 tctttttcca ccgtttcaga tggttcaaca acgtgaggat gccctccaaa gataatatcc
     1981 gctccccaat cgatcatctt gtgataaaga gctttttgtt cttcagttgg ttccaatcga
     2041 tactcaacac ccatctgaag catgataatg gtgatatctg cttccttctc tgcccgttca
     2101 atttcaacct tcatcttatc ttcgtttaaa tctgaaagat aacgattata gtcttcctga
     2161 gaaatatact gctcaattcc attgaaacca taggaatagg ctaacaatgc aaccttgata
     2221 ccattcactt ccttaatgac cagcggagcc tgaacacgtg gttcgtgcgt ataaactccg
     2281 attggagtga ttccagcttt ctcaataata tcggccgttg aaataactcc ctcaagttga
     2341 aataactccc tcaagttgcg aatccaaaat atgattatga gctaaatcca gcacatgata
     2401 acctgcctcc ttaatagcat ccataacttc agcaggagca ttaaagagaa gataacccgc
     2461 taaataatga tccttattaa tggttccttc aaaatcacca atagctaaat ctgcttgctt
     2521 gagccaagga gtcacatact caaaattttc atgaaagtca taggtaccgt cttctttttt
     2581 agctgaaaag aaaagtccat cgtggtagag caaatccccg tgggccataa ttctggcagt
     2641 tttttcctct tcctactcct gagacttttg cttagtcccc tcttgagaaa tagtatctcg
     2701 tttttgacta gtcaagggat tccttggaag ctttcaaaca acaagaccaa gcccttcata
     2761 aaacgaaaag ctaaaccacc ctgatttcga ttttatcttc tttgtatctt cattctccct
     2821 actttcttat gcaagccttt tctttttatt atatcacaga taagtatttc tttcacaatt
     2881 gaattgaact tcccatctat tttctataaa tcctaaatgc cataatgctt tcaattcttg
     2941 tcattttgtg atatcatgta gaggaaatga actaatccac agtggcttat ttcaagtata
     3001 ccacttgggc tttggcagta gctaactgca ctaaatataa tataaggaga gaaaatgtct
     3061 gcaatagaac gtattacaaa agctgctcac ttaattgata tgaacgatat tatccgtgaa
     3121 gggaatccta ctctacgcgc gattgctgag gaagtcactt tccccctatc tgaccaggaa
     3181 atcatcctag gcgaaaagat gatgcaattc cttaaacatt cccaagaccc tgtcatggct
     3241 gaaaaaatgg gactccgcgg tggtgttgga ctggctgctc cccagttaga tatctcaaaa
     3301 cgcattatcg ctgttttggt acctaatatt gttgaagaag gcgaaactcc acaggaagcc
     3361 tacgatttgg aagccattat gtacaatcca aaaatcgtct ctcactctgt tcaagatgct
     3421 gctcttggcg aaggagaagg ttgcctgtct gttgaccgta acgtgcctgg ctatgttgtt
     3481 cgccatgccc gcgttactgt tgactacttt gacaaagatg gagaaaaaca ccgtatcaaa
     3541 ctcaaaggct acaactccat tgttgttcag catgaaattg accacattaa cggtatcatg
     3601 ttttacgatc gcatcaatga aaaagaccca tttgcagtta aagatggttt actgattctt
     3661 gaataaagaa aatcccgttg caagacgggg ttttgtgtta taatagaggc atgaaaacaa
     3721 atgatattgt ctatggcgtc cacgccgtta ccgaagccct ccttgcaaat acaggaaaca
     3781 aactctacct ccaagaagat ctccgaggta agaatgttga gaaagtcaag gaactagcta
     3841 cagaaaagaa ggtgtccatt tcttggacat caaaaaaatc tctctctgag attactgaag
     3901 gtgctgttca tcaaggtttt gttctacgag tgtctgaatt tgcctatagc gagctagatt
     3961 acatccttgc aaaaacacgc caagaagaaa atccacttct attgattcta gatggtctaa
     4021 ccgatcccca taatctgggt tctatcttgc gaacagccga tgcgaccaat gtttcaggtg
     4081 tcatcattcc caagcaccgt gctgtcggag taactcctgt cgttgccaaa acagccacag
     4141 gtgctattga acacgttcca attgcccgag tgacaaacct cagtcaaacc ttagataaac
     4201 tcaaggatga aggcttctgg acctttggaa cggatatgaa cggtactcct tgccacaagt
     4261 ggaatacaaa agggaaaatc gccctcatca tcggaaatga aggaaaaggc atctctagca
     4321 acatcaaaaa acaggtcgat gaaatgatta ccattccgat gaatggacat gttcaaagcc
     4381 tcaatgccag tgttgctgcg gccattctca tgtacgaagt ttttctaaat agactataaa
     4441 aaagtttcca gtcatctgat tggaaacttt tttatgatta actatgttct gtgataaact
     4501 tgtaggcttc ttgaccagcg atagctccat ctccaactgc tgttgttact tggcgaaggt
     4561 ctttcaagcg aacatctcca actgcaaaga taccgtcgac tgcagtcttc atgtggctat
     4621 ctgtcacaat ccagcctgcc tgatcttgga tattcaattc tttaacaaaa tcgctaagag
     4681 ggtccaaacc aacatagata aagacaccac cgaaggcttg ctctgtcact tgacctgttt
     4741 tcacattttc aaaaacgaca gattctactc tgttttcacc cttgatttcc ttcactacag
     4801 aatcccagat aaagctgatt ttttcattcg caaaggcgcg atcttgtaaa accttttggg
     4861 cacgaagttg gtcacgacgg tgaacaatgg taacagtctt agcaaaacga gtcaagaaga
     4921 gggcttcttc aacagctgaa tctccaccac caactaccaa taaatcttgg tcacggaaga
     4981 aagcaccatc acacacagca cagtaagaaa caccacgact gttcagttct tcttctccag
     5041 gcactcccaa aggacggtgt ttagaaccag ttgctacgat aactgtacgt gtttcatatg
     5101 tttggtcatc agtcatcact ttcttaaaat caccatggtc ttcgacattt tcaacataac
     5161 cataaatgtg ctcaacacca agattttcaa gtggttcaaa catcttttca gccaattcag
     5221 gtccactaat attagcgtat cctgggtaat tttcgatatc agatgtatta ttcatctgac
     5281 cacctggcag accaccttca atcaaagcta cttttagatt gcttcgagca gcatacaagg
     5341 ccgcagtcat ccctgcaggt ccagcaccga taataatagt atcgtacata tagattcctt
     5401 ctttcttgtt gtaactatct ttattctaac tctttcttgt caatcacgca aggattatgc
     5461 cttgaaaaca agaattaaac tcataaccgc aaaggataat actgggacaa ccaagactcc
     5521 aatcataatc atatcataga gatgggcttg gcgagccctg gctgtcttat caactcccga
     5581 catggctctc agaccaatcc aaatccctaa aaaaatcaga acaaggatgg tggtcaagat
     5641 caaactctcg aaatataaag aaaatagttg cagtagcatg atttctctca tttctatctt
     5701 ttttaaagag taaactcagc tagtccaact aactgagttt tcctttatct attatatcaa
     5761 atataagtcc gtttgtaact agcgaagaat tcttttgtcc gctcttcttt aggggtgtgg
     5821 ataatctcat ccggagttcc agactcgatg attttcccct tatctaagaa gagaatttta
     5881 tccgcaactt gggctacaaa ggacatgtca tgactgacca aaatcatggt ctgacctgac
     5941 ttagcagcat ctgcaataga cttttctact tcaccgacca attctgggtc aagggctgaa
     6001 gttggttcgt ctaagagcaa aacatctggt ttcatagcaa gcgcacgcgc tagggcaacc
     6061 cgttgcttct gtccacctga taaatggcga ggataatggt tttcacggtc cgaaagccca
     6121 accttagcca actcttcctt ggcaatctta gtcgcttctt ggtcagataa tttcttgaca
     6181 acaaccaagc cttctttcac attatcaagt gctgttcgac gttcaaataa attaaactgt
     6241 tggaaaacca tagacaactt acggcgtagg gcaaggattt cttcttgagt gattttagaa
     6301 aaatcaactg aaaaaccatc aatctgaata gagccactgt caggtgtttc aagataattg
     6361 agactgcgaa gaaaggttga ttttccagct cctgaagaac caatcaaggc tacaacttcc
     6421 cctttttgaa tatccaagtt cagatgatcc aagacagtct gtcctgaaaa ggatttgctt
     6481 aaattcgaaa tcttaatcat taacgaaggt ctcctttcac atctgtttgc actgtatcag
     6541 gtgcagaaat agccattttt ctctcgatga aacgaccgag gctttcaatt ccgatattga
     6601 ctacccaata aacaagggca acagagatga agcgttcaaa atagcgataa tcagctccac
     6661 ctagaatctg agcttgggca aagacttcca caacacccgc actaaaagct agagatgttc
     6721 ccttggtcaa accgatgagg gaattaatca aggttggagt agctaccacc gctgcattag
     6781 gaataatcac tcgtcgataa acttgcgctc gggtcatacc cagactgcgc gccgcctcaa
     6841 tctcaccagg attaactgag agaatggctg cacgaatggt ttcactagca taagctgcct
     6901 cattaaaggc aaaagcgaca atcgcaaaag ctgcagctgg aatcgcattg atattgagac
     6961 cagttcccca ttgctgattg agggctttca aagccaaagg aattccgtag taggtcaaca
     7021 tgagttgcac caaaatcggt gtccctttta agaaactaac aaagaaggcc tgcaagggat
     7081 ataaaatctt gacacgattg atcttcacaa tggcaaaaag aagcgccaaa accaagccaa
     7141 aaagggcacc accaattgtc aacataattg ttgttggaag ttgttggaca attctaggga
     7201 ttccatcaaa gaccgaacgt aggctaaaca gcttgccatc cggaatcaat tgcatcaagt
     7261 tttggtacca atctgatgct aaaatcgttg taacattcat aaaaacatcc tcttctgata
     7321 atgattcatt ctaccatgct tctgccgtca atgaaattat ttgctacggt cagatacaga
     7381 ctttgtctac ctactagttt atcatacttt gaaacaggga agttatatat tttatctatc
     7441 aaagagatag ataaaaacta tttcagtccc agttcttcat aagattttcg ataaccgatt
     7501 tgcttaacag ttccattttc tactaaaatg ggccgtttta acaacatacc gtcacttgct
     7561 agcaactcag ccgcttcttg gtttgacaaa cttcctacct tatcttttag ccctaattca
     7621 cggtatttga taccactggt gttgaaaaat tgcttcaatt caaatcctga ggtttctagc
     7681 caattcaaaa tgacttcttg gctaggtgtt tcttccacga tatggacggc tttatagtcc
     7741 acacctaatt gatttaattc ttgttttgct tttttacaag ttgaacattt ggggtattcg
     7801 ataaattcta acatgtgctt ctccttctat ttgtttatat ctttaaaatc tacgccttca
     7861 tgctccaaga gccaagcttt cttttccact cctgcagcat agcctgtcag acgcttgcct
     7921 gctcccaaca cacgatgaca aggtactagg atagaccaag gattgcgtcc cactgctcca
     7981 ccaattgctt gagcagaagc cacttgcagg tcttgagcaa tttgtccata ggtcactgtc
     8041 tgaccataag gaatgccctg taaatagtcc caaactctct tttcaaaatt cgttccgatt
     8101 ggcgccaaga gcaagttgga taaatcctga ggcttgcctt taaagtaatc atctaagcaa
     8161 gcaataactg ggtctaaaat aggatgacta acaacttctt ctatcgtttc atctcctagt
     8221 cccctctcaa aatgcttctg ctcctgaacc caaattccat acaaataatg gtcatcagct
     8281 acaagtgata gaataccaat tggtgaggag tagaggattt ttacatactt cttttgcatt
     8341 atttctttaa tttttgatag gccaagcgtt cgccatctac tggcacctta ccaacttgtt
     8401 taaaaccaag tttttcaaaa atatgttgca taaccttgtt ttcagcatgc gtatctgagc
     8461 gaaaatcaag ataatcaaaa ccttcaatca agccctctaa gaaggtttga gcaactcctt
     8521 ttccctgcac atctgctgcc acagcaatac ggtgaaagac tagatactct gactctccag
     8581 cttgccagtt tccctcataa atagcttcat aggcctcctc tggactcttg gtcacagcag
     8641 cataggctag cagttctccc tcttccaagg ctacgtaggc ttgccctgag atgatatcat
     8701 cgataataac atctgcattt ggatagccat tttgccactg gttactacca gcatctgcta
     8761 aacatttttt ggcatcctct atcacctgca tgatggcatc tacttcattt ggaaaagcta
     8821 aacgaatttc catctttttt cctcatttct gactagtttc tcccatcata gcataattta
     8881 agtctttcta caagcagtta aaacttgact ttgtattttt gttattttat aatctagtta
     8941 tcaaaactat ataaaaagga gttgaaaatg aaaactacct tttcctaccc aaaatgggca
     9001 gaaattccaa acattaacct ctatctggac caggttttgc tctatgtcaa tcaggtctgc
     9061 gcccctatct ctcctaataa agacaaaggc ctaacagcat ctatggtcaa caattatgtg
     9121 aaaaatggtt acctgacaaa gcctgacaaa aaaaaatacc aacgccaaca gattgcccgt
     9181 ttgattgcta tcacaaccct caagtctgta ttttctattc aagaaatagc ccagacactt
     9241 aatactctac aaactcaagc aagttcagac caactctacg atgcttttgt ggactacatg
     9301 aaccaaggaa ttgacccagc taaccctatt atccaaacca gctgccaaac cgttaaactc
     9361 tatcatcaaa ctctagactt aatcgatcat actcaagagg aggtaatcca atgaacacta
     9421 gtcttaaact cagcaaacaa ctcagttttg gagaggagat tgctaatagc gtgacccatg
     9481 ctgtgggtgc agtcatcatg cttatcttgc tgcctatttc atccatctat agttatgaag
     9541 cacacggatt tttatcctct atcggcgttt ccattttcgt catcagtctc tttctcatgt
     9601 tcctatcatc caccatttat cactctatgg cctatggttc gacccacaaa tatgttttgc
     9661 gaatcattga ccattctatg atttacgttg ccattgccgg ctcatacacg cccgttgtct
     9721 tgaccttgat gaataactgg tttggctatc tgattattgt catccaatgg ggaacgacca
     9781 tctttggtat tctctataaa atctttgcta aaaaggtcaa tgagaaattt agccttgctc
     9841 tttacctgat tatgggctgg ttggttctgg ctatcattcc tgccattatc agtcaaacga
     9901 cacccgtttt ctggagtctc atggtaactg gcgggctctg ttatacagtt ggagctggat
     9961 tttatgccaa gaaaaaacct tatttccaca tgatttggca tctctttatc ctagctgcgt
    10021 ccgcacttca atacatcgct attgtttatt acatgtaaaa aagttgagaa attcaatctc
    10081 aacttttttc tttacacata ttgataaagt actggtgcaa gcgcacatca tcagtcaatt
    10141 ctggatgaaa agaacttacc aacatatttt tttcttgggc tgcaacaatt tgattgttca
    10201 ctgttgctaa aatttctaca ccctcaccaa cactactgat aatcggacca cggataaagg
    10261 tcattggaat cttgccaact cccttacatt ctgcttccgt gtagaaactt cctaattggc
    10321 gcccataagc attacgctcg accaccatat ccatagttcc tagatgactc tctttctgag
    10381 aagtgatttc cttagccagc aaaattaagc ccgcacaggt cccaaacact ggtaagccag
    10441 atagaatggc ttctcggatg ggaagtagca tgttctggtc acgtaagagc ttgcccatgg
    10501 ttgtagactc accaccaggc aaaatcaaac ccgacaagtc actctgatct tgctgaaaat
    10561 catctagatt tctgagttct acactctcga cacctaattg atctagcact tttgcatgtt
    10621 ctgcaaaggc cccttgcaag gccaatattc cgattttcat ctattttcct cgttcagcca
    10681 tgagaatttg gatttcattt tcattaatac caaccatggc ttctcctaaa tcttcagaga
    10741 tttgagctag aatttgagga ttacggaagt tagtcacagc cttaacaatg gcactcgctc
    10801 gtttaacagg atctcctgac ttgaaaatac ctgaaccgac aaagaccccc tctgccccta
    10861 attgcatcat taacgcagca tctgctggcg ttgcaacacc tccagcagcg aaatttacaa
    10921 ctggcaattt tccatgttca tgaacatatt ggaccaattc tacagggact tgcaaatcct
    10981 tggcagcaac ataaagctcg tcctcacgta agttttgaat gcggcgaatt tcctgattca
    11041 tcatacgcat atgacgaaca gcttggacta tatcccctgt ccctggttct cctttggtac
    11101 gaatcatgga agcaccttca gcgatacgac gcaaggcttc acccaaatcc ttagcaccac
    11161 agacaaaagg aacttggaat tctttcttgt ccacatggaa acggtcatca gctggagata
    11221 gaacttcact ctcgtcgata taatcaattt caatagcctc taaaatctga gcttcaacaa
    11281 aatgcccgat tctgacctta gccattactg gaatactaac cgcttcttgg atttccttaa
    11341 tcatctttgg gtcgctcatg cgggaaactc ctccagctgc acgaatatca gccggaattc
    11401 gttccaaggc catcacagct gccgcaccag cagcttctgc gatacgagcc tgttcaggat
    11461 tctgcacatc cataataaca ccacccttga gcatctgtgc caagttttta tttagttcat
    11521 aacgattttc agtcatttgt tttatcctca tcatttgtat tggtagctac cttttcattt
    11581 taagtcagaa cagaatgaag cacaagataa aaaaactata attgtatggg gacagattag
    11641 ctaaaaacta tctgacctta acttaaggcc agatagctca ttcattttta tttttcagcc
    11701 gtaagggcag ccattgtgat gtagttgtat ggtttgttga agtgtggcaa gaagaagagg
    11761 tctgtcaatg ccaatttatc aattgtcaca tgctcttgga tagcaagtga gaacatgtgg
    11821 attcccatgc taattgcaat atcatgtgaa accatttggg caccaagaat ttcacggcta
    11881 tctttgtcaa agacaatctt aattgctact tcatggttgt catgtttcat gaattctggt
    11941 ttttgaagat cgttaaagcc tgtttcagtt gcgttgtaac cagcagcttt cgctttttca
    12001 agagtcaaac cagttgaaac catgtgaaga ccgtagattg agataccatt tgaaccttga
    12061 acaccgattc cttccaattc atgtccacaa gcattgtagg caccaacgat accagtgcga
    12121 acagcatttg aagcaagagc gatatagctt gtatctttac gagcattgtc ataaacagtc
    12181 gcacagtcac caacagcgta aacgcctggg atagatgttt cttgtttctt gtctacaagg
    12241 aaggcaccgt tgcggaagag ttcgatctta ccatctgcaa gggctgtgtt tggacggaaa
    12301 ccaactgcaa ggataaccat atccacgtca aagctttctt tgtcagtaat caagcgttca
    12361 actttaccgt caccttcgat tgctttaaca gtttgaccta gagccaagcg gatgttgtga
    12421 tcttccaagt tcttcgccat catttgtgtg aagtctttgt catagtaacc gttcaagaca
    12481 gtatcaacga tatcaacaag gacaacttct tttccaagac gttcaaaggc ttcagcaagt
    12541 tcaacaccga tgtaaccacc accaacaacg gcgatacggt cgaggtgttg gctcttgtca
    12601 gaaagtttat tgataacttc ttcagcattt tggtacaatt tcacgaattg tacgttttca
    12661 agagttgctt taaattcgcg gtttccttta acaatttcaa caccttcgat tggtggcaag
    12721 attggtgtag aacctgtagc gaaaatcaat ttttcgtatg attctttgtg ctcttttcct
    12781 tcaacttccg ctgtaactac tttgttatca tagtcgattg aaagaacagg tgagttcatg
    12841 taaactttag cacctttagc ttccaatttt tctttatcag aatagaacaa gccttcagca
    12901 ccgtcaattt gttcaccaat ccaaagagcc attccacatc ctaggaaaga gatgttagag
    12961 ttttggtcaa atacaacaat ttcgttctca tttccaaaat tatccaacat ggtattgata
    13021 catgctgtac cagcgtggtt agcaccgact acaacgattt tactcataaa aatttcctgc
    13081 cttcattcta aatttacctt actagtatac catttaccgt tagcgtttac aaggggggta
    13141 ctattatttc agcatttttt atcaaaaata attccccatt tatcctgttt tcaagcctca
    13201 tgaattttat gagcatattt cttgactttt tccaaatttt gtttgtgaaa at
//
LOCUS       DAVMGZ010000044        13158 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000044 DAVMGZ010000000
VERSION     DAVMGZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.045"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            32..862
                     /locus_tag="VYQ93_001055"
     CDS             32..862
                     /locus_tag="VYQ93_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3335755.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NSSPKPFNYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAV
                     NNISYTKERAEKYLYAAPTVKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQL
                     EDYNKQHSDNPTILNYTKANLQQIMGHLSDGQFDYKIFDKITVETVIKNQGLDNLKVI
                     DLPSDQQPYVYPLLAKGQDELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            903..980
                     /locus_tag="VYQ93_001056"
                     /pseudo
     CDS             903..980
                     /locus_tag="VYQ93_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
     gene            1084..1914
                     /locus_tag="VYQ93_001057"
     CDS             1084..1914
                     /locus_tag="VYQ93_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3335756.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2068..2922
                     /locus_tag="VYQ93_001058"
     CDS             2068..2922
                     /locus_tag="VYQ93_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV3335757.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3022..4395
                     /locus_tag="VYQ93_001059"
     CDS             3022..4395
                     /locus_tag="VYQ93_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV3335758.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4388..5449
                     /locus_tag="VYQ93_001060"
     CDS             4388..5449
                     /locus_tag="VYQ93_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV3335759.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5451..6143
                     /locus_tag="VYQ93_001061"
     CDS             5451..6143
                     /locus_tag="VYQ93_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3335760.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6173..6742)
                     /locus_tag="VYQ93_001062"
     CDS             complement(6173..6742)
                     /locus_tag="VYQ93_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV3335761.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPTPGTV
                     LLFIASIVIEALVGALIGQALSKKFAQKI"
     gene            6877..7491
                     /locus_tag="VYQ93_001063"
     CDS             6877..7491
                     /locus_tag="VYQ93_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV3335762.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7493..9139
                     /locus_tag="VYQ93_001064"
     CDS             7493..9139
                     /locus_tag="VYQ93_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3335763.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9151..10437
                     /locus_tag="VYQ93_001065"
     CDS             9151..10437
                     /locus_tag="VYQ93_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3335764.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10489..11022
                     /locus_tag="VYQ93_001066"
     CDS             10489..11022
                     /locus_tag="VYQ93_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3335765.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11100..11570
                     /gene="nrdI"
                     /locus_tag="VYQ93_001067"
     CDS             11100..11570
                     /gene="nrdI"
                     /locus_tag="VYQ93_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV3335766.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(11863..13125)
                     /locus_tag="VYQ93_001068"
     CDS             complement(11863..13125)
                     /locus_tag="VYQ93_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV3335767.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         3971 a         2460 c         2983 g         3744 t
ORIGIN      
        1 ttataaggta ttctattttg gaggaaatga catgaaaaaa atcgttaaat actcatctct
       61 tgctgcccta gggcttgttg ctgcaggtgt gctagcagct tgctcaggtg gtgctaagaa
      121 agaaggacaa gcagctagca agaaagaaat tattgttgca actaattctt caccaaaacc
      181 attcaactat gaagaaaatg gcgaattgac tggttacgag attgaagtcg ttcgcgctat
      241 ctttaaagac tctgacaagt atgatgtcaa gtttgagaag acagagtggt caggtgtctt
      301 tgcgggtctt gacgctgacc gttaccaaat ggctgttaac aatatcagct acactaaaga
      361 acgtgcggag aaataccttt atgcagcccc aactgttaaa gaccctaacg ttcttgtagt
      421 caagaaagac gattctagca tcaaatcgct tgatgatgtt ggtggaaaat caacagaagt
      481 tgttcaagga acaacttctg ctaaacagtt agaagactac aacaaacaac actcagataa
      541 tccaactatc cttaactata ctaaagcaaa cttacaacaa atcatgggcc acttgagcga
      601 tggtcagttt gactacaaga tttttgataa aatcactgtt gaaacagtca tcaagaacca
      661 aggtttggac aacttgaaag tcatcgatct tccaagcgac caacaaccgt acgtttaccc
      721 acttcttgct aaaggtcaag atgagttgaa aacatttgta gacaaacgta tccaagaact
      781 ctacaaagac ggaactcttg aaaaattgtc taaacaattc ttcggagaca cttacctacc
      841 ggcagaagct gatattaaat aatttcttga aatcatccat tctgagtaag atgggtgatt
      901 tttcagttcg ctactgcaat ctagtcggaa cagttctctt tggagtggtc ttgattgcca
      961 tcttgcgttt ggtcttttaa attacccata aaaaaaactt atcacagaga tagatatcat
     1021 ataatggcgt aaatgctctt tttctgttaa gattataagg tattctattt tggaggaaat
     1081 gacatgaaaa aaatcgttaa atactcatct cttgctgccc taggacttgt tgctgcaggt
     1141 gtgcttgcgg cttgctcagg tggtgctaag aaagaaggag aagcagctag caagaaagaa
     1201 atcatcgttg caaccaatgg atcaccaagg ccatttatct atgaagaaaa tggcgaattg
     1261 actggttacg agattgaagt cgttcgcgct atctttaaag attctgacaa atatgatgtc
     1321 aagtttgaaa agacagaatg gtcaggtgtc tttgctggtc ttgacgctga ccgttacaat
     1381 atggctgtca acaatcttag ctacactaaa gaacgtgcgg agaaatacct ctatgccgca
     1441 ccaattgccc aaaatcctaa tgtccttgtc gtgaagaaag atgactctag tatcaagtct
     1501 ctcgatgata tcggtggaaa atcgacggaa gtcgttcaag ccactacatc agctaagcag
     1561 ttagaagcat acaatgctga acacacggac aacccaacta tccttaacta tactaaggca
     1621 gacttccaac aaatcatggt acgtttgagc gatggacaat ttgactacaa gatttttgat
     1681 aaaatcggtg ttgaaacagt gatcaagaac caaggtttgg acaacttgaa agttatcgaa
     1741 cttccaagcg accaacaacc gtacgtttac ccacttcttg ctcaaggtca agatgagttg
     1801 aaatcatttg tagacaaacg catcaaagaa ctttataaag atggaactct tgaaaaattg
     1861 tctaaacaat tcttcggaga cacttatcta ccggcagaag ctgatattaa ataatttctt
     1921 gaaatcatcc attctgagta aggtgggtgg tttctcagtt tttagggata tagttttaaa
     1981 ctatatatct gactatctaa taacaatttg tgaaatcaaa caaattgtga gatactagta
     2041 cggtattatt tttaaggaga aagaatcatg aaaatcaaaa aatggcttgg cctagcagcc
     2101 cttgctacag tcgcaggttt ggctcttgca gcttgcggaa actcagaaaa gaaagcagac
     2161 aatgcaacaa ctatcaaaat cgcaactgtt aaccgtagcg gttctgaaga aaaacgttgg
     2221 gacaaaatcc aagaattggt taaaaaagac ggaattacct tggaatttac agagttcaca
     2281 gactactcac aaccaaacaa agcaactgct gatggcgaag tagatttgaa cgctttccaa
     2341 cactataact tcttgaacaa ctggaacaaa gaaaacggaa aagaccttgt agcgattgca
     2401 gatacttaca tctctccaat ccgcctttac tcaggtttga atggaagtgc caacaagtac
     2461 actaaagtag aagacatccc agcaaacgga gaaatcgctg taccaaatga cgctacaaac
     2521 gaaagccgtg cgctttactt gcttcaatca gctggcttga ttaaattgga tgtttctgga
     2581 actgctcttg caacagttgc caacatcaaa gaaaatccaa agaacttgaa aatcactgaa
     2641 ttggacgcta gccaaacagc tcgttcattg tcatcagttg acgctgccgt tgtaaacaat
     2701 accttcgtta cagaagcaaa attggactac aagaaagcac ttttcaaaga acaagctgat
     2761 gaaaactcaa aacaatggta caacatcatt gttgcgaaaa aagattggga aacatcacct
     2821 aaggctgatg ctatcaagaa agtaatcgca gcttaccaca cagatgacgt gaaaaaagtt
     2881 atcgaagaat catcagatgg tttggatcaa ccagtttggt aataagaaac agggaggtgg
     2941 gagagaaaat tccacctctt gcttttgtat agagtataga ttgtaaagaa gactattcgt
     3001 tcatagaaag gtagagagaa tatggttttt cctagcgaac aagaacagat tgaaaaattt
     3061 gaaaaggatc atgtagccca gcattatttt gaggttttgc gtaccttgat ttctaagaaa
     3121 tcagtctttg cccagcaggt tggactcaag gaagtcgcaa attatctggg tgagattttc
     3181 aagcgtgttg gagctgaagt ggagattgat gagagctata cagcgccctt tgtcatggca
     3241 catttcaaga gttcgcgtcc agatgccaag accttgattt tctataacca ctatgacact
     3301 gtgccagcgg atggggatca ggtctggaca gaggatcctt ttacgctttc ggtccgcaat
     3361 ggcttcatgt atgggcgtgg ggttgatgac gacaagggtc atatcacagc tcgcttgagt
     3421 gctttgagaa aatatatgca gcaccatgat gatttacctg tcaatatcag ctttatcatg
     3481 gagggagcgg aggaatcggc ttcaacagac ctagataagt atttggaaaa gcatgcagac
     3541 aaactccgtg gggcggattt gttggtctgg gaacaaggga ccaaaaatgc cttggaacag
     3601 ctggaaattt ctggtggcaa taaggggatt gtgacctttg atgccaaggt aaaaagcgct
     3661 gatgtggata tccactcgag ttatggtggt gttgtggaat cagctccttg gtatctcctc
     3721 caagccttac agtctcttcg tgctgcggat ggccgtatct tggttgaagg cttgtacgaa
     3781 gaagtacaag agcccaatga acgagaaatg gccttgctag aaacttatgg tcaacgaaac
     3841 ccagaggaag ttagtcggat ttatggattg gagttgcctc tcttacagga ggagcggatg
     3901 gcctttctaa aacgtttctt tttcgagcca gcgctcaata tcgaaggaat ccagtctggt
     3961 tatcaaggtc agggtgttaa gactatttta cctgcagaag ccagtgccaa gctagaggtt
     4021 cgtctggttc cgggcctaga accgcatgat gttctggaaa aaattcggaa acagctagac
     4081 aaaaatggct ttgataaggt agaattatac tataccttgg gagagatgag ctatcgaagc
     4141 gatatgagcg caccagccat tctcaatgtg atcgagttgg ccaagaaatt ctatccacag
     4201 ggcgtttcag tcttgccgac gacagcgggg acaggaccta tgcatacggt ctttgatgcc
     4261 ctagaggtac caatggttgc attcggtcta ggaaatgcca atagccgaga ccacggtgga
     4321 gatgaaaatg tgcgaatcgc tgattattac acccatatcg aattagtaga ggagctgatt
     4381 agaagctatg agtagagata ttatcaagtt agatcagatc gatgtgactt ttcaccaaaa
     4441 gaagagaacc atcacagcgg ttaaggatgt gaccattcac atccaagaag gggatatcta
     4501 cggaatcgtt ggatattctg gagcaggaaa atcaaccctt gtacgggtga ttaatctctt
     4561 gcaaaaacca tctgcaggga aaattaccat tgacgatgat gtgatttttg acggcaaggt
     4621 gaccttgacg gcagagcagt tgcgtcgtaa acgtcaagat atcggaatga ttttccagca
     4681 ttttaacctg atgagccaaa agacagcaga ggagaatgta gcctttgccc ttaaacactc
     4741 tgaactcagc aaggaagaaa agaaggctaa agtagctaag ttgttggact tggttggttt
     4801 ggcagatcgt gctgaaaact acccttcaca actatctgga gggcaaaaac agcgtgtggc
     4861 cattgcgcgt gccttggcca atgatccaaa aatcttgatt tcagacgagt caacttctgc
     4921 ccttgatccg aagacaacca agcagatttt ggccttgttg caagatttga accaaaaatt
     4981 aggcttgact gttgtcttga ttacgcatga aatgcagatt gtcaaagaca ttgccaaccg
     5041 tgtggcggtt atgcaggatg ggcatttgat tgaagagggt agtgtgcttg aaatcttctc
     5101 agaccctaaa caacctttga ctcaagactt tatctcaaca gctacaggta ttgacgaagc
     5161 catggtcaaa atcgagaagc aagaaatcgt ggaacacttg tctgaaaaca gtctcttggt
     5221 gcaactcaag tacgctggag cttcaacaga cgagccactt ttgaatgaat tgtacaagca
     5281 ttaccaagta atggctaata ttctctatgg gaatatcgaa attctcgatg gtactcctgt
     5341 tggagaattg gtggtggtct tgtcaggtga aaaagcagcg ttggcaggtg cccaagaagc
     5401 cattcgtcaa gcaggtgtac aactaaaagt attgaaggga gtacagtaag atggaatcat
     5461 tgattcaaac ctatttacca aatgtctata aaatgggttg ggctggtcag gcaggctggg
     5521 gaacggctat ctacttaact ctttatatga cagttctttc cttcattatc ggaggcttct
     5581 tggggctagt ggcaggtctc tttctcgtct tgacagcgcc aggtggtgtc ttggagaata
     5641 aagtcgtatt ctggatttta gacaaaatta cctcaatttt tcgtgcggtt ccctttatca
     5701 tcctcttggc aatcttgtca ccactttctc acttgattgt taaaacaagt atcgggccaa
     5761 atgcagccct tgtcccactt tcttttgcag tctttgcctt ctttgcccgt caggtgcagg
     5821 ttgtcttggc tgaattggat ggcggtgtca ttgaggcggc tcaagcgagc ggagcgactt
     5881 tctgggacat cgtgggtgtt tacctatcag aaggtcttcc agatttgatc cgtgtgacga
     5941 ctgtgacctt gatttccctt gttggggaaa cagctatggc cggtgcggtt ggagctggtg
     6001 gtatcggtaa cgtagccatc gcttatggat ttaaccgcta caatcacgat gtgaccatct
     6061 tggcaaccat tattatcatt ttgattatct ttgcaatcca attcttggga gatttcttga
     6121 ctaagaaatt gagccataaa taaaaaagag ccgcgtggct ctttttaact gatcagattt
     6181 tctgtgcaaa ttttttactc aaggcttgtc caatcaaggc acccactagg gcttcgatga
     6241 caatacttgc gataaataga aggacagttc caggggttgg agcgaccatg atacggtcga
     6301 tatattcttg ggattttcct cttgccagaa gagtagccat ataggctttg ggcgcaatcc
     6361 acataagcaa gattggtcct gttgtactaa aggcgaaaat aatgaaagaa aggaagttct
     6421 ttgttttgtc cttgtatttt cctaaatgag ctactccatc tgctaggagg ccacagataa
     6481 ttccaggaag gaaggaacca gcaccgtgtt tagttcccaa gaaaaagagg gcaatgacaa
     6541 ggccgatagt ggtaatggct ccaaagcgcg gaacttttgc gactaggatc atatagacgc
     6601 taccgccgac aagggcagta aaggcaggcg cataaaacat gtttccagtt tggtcaaaga
     6661 gattgcccaa aaggacacca atccccatgc agaggaagta aagaactgca aaaagcagtg
     6721 tagttaagat agatttcttc atgaattgtc tcctgataat tttttcacaa ttctcatttt
     6781 accatggttt ggtgggattg taaatggata ctagaagttt ttgaaaacgc ttgaaatatg
     6841 atataatggt ttcatagtta tttttagggg aatatcatgg gtagattttt agactttgtc
     6901 tttaatcgtt tctttttagg gatgattgcg acagccttct tttggctatt aactttagca
     6961 ggagggatta tccttggtct agcgccggct agtgccacct tgatgagctt atatgcagaa
     7021 catggttata gctttcggga atacagtttg aaggaggctt ggtctcttta caagcaaaat
     7081 tttgtctcaa gcaacctgat tttctatagc tttttaggtg tgggtctagt tttgacctat
     7141 ggtttgtatc tattggtgca attacctcat cagaccattg ttcatttgat tgcgaccctt
     7201 ttgaatgtcc tagtagttgc cctgatcttt ttggcttata cagtatcttt aaaattacaa
     7261 gtttattttg ccttgtccta tcgaaatagt ctcaaattat ccttgattgg catctttatg
     7321 agtctagcag ctgtggccaa ggttctcctt gggactgtgc tacttgtagc aattggttac
     7381 tatatgcctg ccctgctatt ttttgtagga attgggatgt ggcatttctt tatcagtgat
     7441 atgttggaac ctgtctatga aatcatccat gaaaaattgg cgacaaaata gaatgaagca
     7501 gttttggcta catacgcttc taagaaccta tagttcagtg atgatcatta tcattgcgag
     7561 ttttgcaatc ttactctctt acgctgactg ggattcacgt gaaaaggaag cccagagagt
     7621 agcccagcgt gtaactgcta gaacagtgag tgaaattgaa tattaccata gagagtcaac
     7681 ccagatagct caggctttag ttgaaaatca agctcgtatt gagggaatct ataaatactt
     7741 tagccttagc atgccagact atttttactg gcaattagag cggaaagctt cgccttatat
     7801 atcagtctct ctgtatgaaa atgttgatga cctctatgtt cgaaatgatt ttgtaactgg
     7861 agtggccatt gcttttcaag attacaagga agtctatgtt tctactaaag acaaacgtag
     7921 tggagaaaaa atcagggctg aggatttcaa accagcagga aatagttttg ccattccggt
     7981 gtcagatcca gtgtcagatc aagatttagg agtgatttac atctccttgg atcctgctgt
     8041 tttataccat gccattgata atactagagg tcatactccg atggcagtaa cagtgacctc
     8101 accttttgat acggagattt ttcatattgg tgagacagtt gataaggaga gtgaaaattg
     8161 gctagttggc ttaacttctc atggatatca ggttcaggtg gcagttccta aaaactttgt
     8221 tttacaagga acagtgacta gctctgcttt gattgtgggt ttgagccttc tctttattgt
     8281 cattctttat ctgactttga ggcagacttt tgctaattac caaaagcagg tagtggattt
     8341 agtagaatcc attcaagtca ttgctcaagg cgaagagggg cgtcggattg acatttccga
     8401 gaaagatcag gaattactcc taatcgcgga gacgaccaat gatatgttgg atcgattgga
     8461 aaagaatatc catgatattt accagttaga gcttagtcaa aaagatgcca atatgcgagc
     8521 cttgcaggcg caaatcaatc ctcattttat gtataatacg ctggagttct tgcgcatgta
     8581 tgcagttatg cagagtcaag atgagttggc agatatcatt tatgaattca gtagtctctt
     8641 gcgtaacaat atttccgacg aaagagagac cctcctcaaa caggaattag aattttgccg
     8701 taaatacagc tatctctgca tggttcgcta tcccaagtcc attgcctatg gtttcaagat
     8761 agatccagag ttagagaata tgaagattcc caagtttacc ttgcaaccgc tggtagaaaa
     8821 ctatttcgcg catggtgttg accacaggcg gacagataat gtgattagca tcaaggctct
     8881 taaacaggat ggttttgtgg aaattttggt ggtcgataat ggtagaggaa tgtcggctga
     8941 aaagttggca aatatccgag aaaaattaag tcagaaatat tttgaacacc aagccagcta
     9001 cagtgatcaa aggcagtcta tcgggattgt caatgtacac gagcgttttg tgctctattt
     9061 tggagaccgc tatgccatta ctatagagtc tgcagagcaa gccggtgttc agtatcgtat
     9121 tacaattcaa gatgagtaga aagggagaaa atgtataaag tattattagt agatgatgag
     9181 tacatggtga cagaaggtct gaagcgtttg attccctttg ataagtggga tatggaggtc
     9241 gtcgcaacag ccagtcatgc cgatgaagct ctagaatatg ttcaggaaaa tcctgtcgat
     9301 gtcatcattt ccgatgtcaa tatgccagac aaaacagggc ttgatatgat tcgggagatg
     9361 aaagagatct taccagatgc tgcctatatc ctgctctcag gttatcagga gtttgattat
     9421 gtaaaaagag caatgaacct tagtgtggtg gactatttgg tcaagcctgt tgataaggta
     9481 gagctgggaa atctgctgga gaagattgca ggtcagctcg gcgagagagg gaagaaaagt
     9541 cagactctta gtcaagaatt agacgaggct ggatttgtta gttatttagg ggataaggag
     9601 aattggtgga taggtctatc caaggaaaaa caaggttcct tcaccattcc ctactatgtc
     9661 ttgggccaag actggcagat tttcatttct gaccaacccc tagatggttt agtcgttaca
     9721 ccttttgaag ctccttatca agaacacttt gaacgctgga agctgaatgc tgagaaaacc
     9781 ctcttttacg gttctgtaaa tttgcagcag tctgagagtc tctttgccta ttacgaaccg
     9841 atttataggg ttattattca gggaaatctc aatcaaatcg tagaagagtt aaatctcttg
     9901 gagaaggtag ttcttgaaaa tacgccgcgt gttccgatta ctaaacagct ttttattcag
     9961 tttgtcatgg atgttttcca tttatttgaa catctcaaag ctgatgacct gacagacatc
    10021 gtcaaaacca ttcatgctat tcaatccttc gatgaattgg ttccttatat caaggaaact
    10081 ctgagcagct ttttcggtca ataccgtatg aatgaaaatg tggtcagtgt gctggaagtc
    10141 attggtcgtg attaccaaaa agagctttcc ctcaaggata tcagtaaggc tctctttatc
    10201 aatcctgtct atctagggca gttgattaag cgtgaaaccg attcgacttt tgcagagtta
    10261 ctaaacaaac aacgtattaa ggctgcccaa caacttttgc tttcaactag tgacagcatc
    10321 gaagatattt gttatgctgt tggttacagt aaccttggat atttctataa agttttccga
    10381 aaattgtgcg gaaaatcgcc aaaagcctac cgaaaacagg tagaaactat actataagat
    10441 ttgtattcct ttacaaaagg tgctataata ttatcaggag gtcctatcat gaaaaagaaa
    10501 ccaatttatc tatgggtctt gctaatcttg tctgccctta tttcagttcc gtctctgttt
    10561 ggaattgtga gtcccttgcc tagtaaagaa gcccttcgtg ctgctcagaa acaagttgca
    10621 ggggtcaatg ctcagcaatt agaagaccag cttaattata cctatagagt agcagaagca
    10681 tctcattcta tttttaatgt tgctttgatt gtgctatcta ctattttagt ggtcgtagcg
    10741 attgtcttcc ttgttcgtaa aaatttgcaa tatgcaaact atacttacgt tggctatgtt
    10801 ttgttagcta ttattggttc gatttatggc tatgttggtt tacaagacgc tgtgcagttg
    10861 gttcaagatg agagcatgcg tttgacagtg agtattggtt caaaagctgt tagcattttc
    10921 tatatcgtca tcaatgttct cttcctagcc cttgtcttct ataagatgtg gcgacaacag
    10981 aaagccttgg cagaagaaga ggaaacagaa gagcttacct aagtattgac aaaaaagaac
    11041 tatcaagata caatcttggt agttgttttt tgattataga taaaatcatg gaggtacata
    11101 tgaagacaat ttctttagtt tatattagtt tgagtggcaa cactgaaagt tttgtgacgc
    11161 gcttgaaaga ctatctcttg tcccagtaca aagggattga ggttcaaaag attcatatca
    11221 aggatttggt caaggaaggc aaaaatttct atgaaatgga ccatccctat gtcgcttttt
    11281 tgccgaccta tctcgaaggt gggaatggcg tggataacgg agatgttgag attttgacga
    11341 caccggtggg agattttatt gcctatggta acaatgctag taagtgtttt ggtgtggttg
    11401 ggtcaggaaa tcgtaacttt aataaccaat actgcctaac agccaagcaa tacagtcaac
    11461 gctttggctt ccctgtattg gctgactttg aaatgcgagg catgctggaa gatatcaaac
    11521 atgtcgcagc tattatcgca gatttgtatg agttggaaaa ggagaattaa tactcaatga
    11581 aaatcaaaga gcaaactagg aagctagtcg caggttgctc aaagcacagc tttgaggttg
    11641 cagatagagc tgacgtggtt tgaagagatt ctcgaatagt ataaagttct tataagatgt
    11701 ggttgaagtt gttcaactgt tttttgagta taaacagtct ttaagaacgt aaaaaagcat
    11761 aagttcaggt cttcaaaacc ttgttcttat gctttttatc atagaaatat ttgctgaatt
    11821 ttctcttgaa atgaaacctt atcttcatct cgcgcttgtc ccttaccctg cttttccgat
    11881 tgcgagtgcg tagataaaga agatggcgaa gccaaagagg aaaatcaatc ccgcaatggc
    11941 aaggtgtttg agccaagaag gaagattttt gttttcacgt gttaccaagg cgtaattcaa
    12001 aagagcaaag aaaggtgttg tcaggaaaga gccaatcatg gcaaagcgga gcatggttga
    12061 aacctgacca gcgaagaact tgataatgac gataccgatg atagcagtga tggtcatcca
    12121 gatgttcaaa gatttacgat tgtcctcttt ttgactgatt agcagtcgga gagattcctg
    12181 attaacgcga gaatagccat cgataacagt tataactgtt ccaaagatac agaggaaggc
    12241 aataaaggta atcaagtaac gggaccattc gccaagaaca gaggcataca tgcccacgaa
    12301 ttgagagatg tatttggctg aagcagcttc aaccgcctgc cctgtaggat actgaatcag
    12361 tgctcccagt gccacaaaga agacggctag gatagctgtt ccaatataac cagtgttaaa
    12421 gtcaaacaga gcgtcttctg tgttaaagtt gacggtcttt ctcttttcag ctgaccaaag
    12481 tgaattgatg gctgaaattt caataggagc cggcatccat cctaggaggg agacgatgaa
    12541 gggcagagct gccatttgcc aaggtgtctt ctcgacaaaa tcagaactgt attctggatg
    12601 cttgaccgcc gcaatgataa ctgcaagaac agtcgcaatg gttaaggcag acataatcca
    12661 tttgaccatg ccgtctaaaa gtttgtagcc tccaaagagt agcatagccc aaatgattgc
    12721 aacgagaatg agggaccact gagtaatgct aagtccaatc attgggaagg cactggcgat
    12781 gatagctgag cacagaatgg caacaccagc cgtgttgacc atagccgaaa agacattgag
    12841 aataaagaaa atccagagat agagttttcc tttttcggca taaccttcaa ccaaagtctt
    12901 tccagtatca gctgtgtatt cagcaccaaa acggaaaaat ggatatttaa agacattggc
    12961 taagatgacc aagagaagta gagaccaacc gtaagaaccg ccagcttgag ttgaggatac
    13021 aatgtgggaa cctccaacag cggcagttgc cattaggatt ccaggtccca ttgcttttag
    13081 cttgcttgcc caggttgatt gatttaagga aatagcttgc gacatgataa atactccttt
    13141 ttgaattttc agaataat
//
LOCUS       DAVMGZ010000045        13107 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000045 DAVMGZ010000000
VERSION     DAVMGZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.046"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(316..2043)
                     /gene="ezrA"
                     /locus_tag="VYQ93_001069"
     CDS             complement(316..2043)
                     /gene="ezrA"
                     /locus_tag="VYQ93_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV3335768.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIDEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASYVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2125..4071)
                     /gene="gyrB"
                     /locus_tag="VYQ93_001070"
     CDS             complement(2125..4071)
                     /gene="gyrB"
                     /locus_tag="VYQ93_001070"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV3335769.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4086..4649)
                     /locus_tag="VYQ93_001071"
     CDS             complement(4086..4649)
                     /locus_tag="VYQ93_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV3335770.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4734..5288)
                     /locus_tag="VYQ93_001072"
     CDS             complement(4734..5288)
                     /locus_tag="VYQ93_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV3335771.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5299..6522)
                     /locus_tag="VYQ93_001073"
     CDS             complement(5299..6522)
                     /locus_tag="VYQ93_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV3335772.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6559..9009)
                     /locus_tag="VYQ93_001074"
     CDS             complement(6559..9009)
                     /locus_tag="VYQ93_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV3335773.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9209..10138)
                     /locus_tag="VYQ93_001075"
     CDS             complement(9209..10138)
                     /locus_tag="VYQ93_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV3335774.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVHKLHPKNRFLDTGFYDYDPDMVR"
     gene            10278..10565
                     /locus_tag="VYQ93_001076"
     CDS             10278..10565
                     /locus_tag="VYQ93_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV3335775.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10599..11933)
                     /gene="ciaH"
                     /locus_tag="VYQ93_001077"
     CDS             complement(10599..11933)
                     /gene="ciaH"
                     /locus_tag="VYQ93_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV3335776.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11923..12597)
                     /gene="ciaR"
                     /locus_tag="VYQ93_001078"
     CDS             complement(11923..12597)
                     /gene="ciaR"
                     /locus_tag="VYQ93_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV3335777.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            12815..>13107
                     /locus_tag="VYQ93_001079"
     CDS             12815..>13107
                     /locus_tag="VYQ93_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3335778.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVK"
BASE COUNT         3999 a         2798 c         2325 g         3985 t
ORIGIN      
        1 acttcgttat tggacggtta ccaagttact caaagtacag atttgaagct gcagataaga
       61 ctgacgaaat cactttaata ctaaatgaaa atcaaaaagc aaactaagaa gctagcctca
      121 ggctgtactt gagtacggta aggcgacgct gacgtgattt gaagagattt tcgaagagta
      181 taaaacactg ttttgaggtg gtggataaat cggctgggta aaaactcaat ctcaggagaa
      241 aatgaagtaa atctccccac aactatagaa aaagatttga ttctgctcct cacacaataa
      301 aatctttttc ttttattaaa aacgaatcgt ttcacgtgtt ttctcatatg aggtaacaaa
      361 gcgattggtt acaccaggct ctgccacttc caatgcttga gaaatcttgt caaatgaagc
      421 gtgataatca aattcttttt caaaaatatc taaagcttcg ttaaatgctt cttgaatgcg
      481 ttcatcaaat gagcgatagc ggttagaata ttgcaagagt tgctctgtca aagttgcata
      541 ttgtacaata ttataagttt ccgtttctaa agcttccata tcattcgttg caatttcaag
      601 aactcgggta acagattcaa tgttaatcat tttttgttct aactcaacca ttaaatcctc
      661 ggtattattg cttgccgtaa agaataactt caagaaagtt tgtggaatac ctggcagatt
      721 gcgtttttcc atgtatcgct tgatagtatg gagacgattg acataaacat tggccttttg
      781 acgtgcatta atatcatctt tctcaatttg tgtcaggcgc tcactaactg aaatttgctc
      841 atcttcaata tcttttagtt gagtttgtaa atcctcaaga ttttcttcaa gaactgaata
      901 agcttgggtt ggttcttctt gatttgaagt tacctcaaca atagctgcct caaaactctc
      961 taattctgtc tgaatacgac gaacatagct tgcagctgtc tcaggaagta aataggtctt
     1021 gttcaaacgt gcaatatctt ctcccaataa agtattattc tctttcatat gttgaaggta
     1081 agttggaaga gttgcaagta gattttccac tactttctga gcagcaattt ctcgagtaaa
     1141 aatatcatac aaggcattga tttcctcttg tgcctgtcca ttctcatatt cggcattatc
     1201 caattccaac tgacgaatat tctcttggtt tttcttgaat gcttcataaa gcaagtggaa
     1261 acgcgcttca atatccgttt caacaaaatg ataattagca tcaattagtt tacgataacc
     1321 ggattccaaa tcctgtaatt gatctggcaa ttctgtagaa agcgtcgtaa ccaaggctgg
     1381 aacacgatcc acaatatgac ttaaggccaa aatgtgattt tctgtattat ccaaaatcac
     1441 tgcggcttcc acagggtcac ccgatgaatt caaggttaca aattgtgaaa attcagattg
     1501 gatattttct aattgttttt caatttcatc caaggcttga ccatactgtt ctgaattttc
     1561 agcaactcta tgctgaagtt cctcaaataa atccaaagca tgaagaacac gaccactatt
     1621 tttagattct tgcttctcta agtctgccaa agcattgcga attgccgcaa tatcttcgtc
     1681 aatcaaagta atttgactct caatttggtc aatttgatga ctggccttga gaaaacgaaa
     1741 tgaatggtta tagccttctg cttcaaagag attattttca atatcggcaa aagagttgag
     1801 agataaatcg acccattttt gattccattc acggaaagcc acttgacttt gtccaatcaa
     1861 gtgcatattt tttacagctt ctacttcatc atttactgga agattgtata gttcttcttt
     1921 tctttcttct agcgcctcta atctcccctc gtttcgctta cgtagaaaga ttgccactac
     1981 ataagccaga actaaaatga ctgcaattgc aaccattaaa taaattagtt gtccattaga
     2041 catatcaaac tcctttttta cttgaaacaa tcgtaacgat tatatcatat tttttagacc
     2101 aagggaacta cttctcccta ttttttagac atcaagtgta ctatagacag cattttcttc
     2161 gataaactca cgacgaggct ctactcgatc ccccatcaac atatcaaaga ttttatctgc
     2221 ttctgcagca tcatctacag aaactctagc catcaagcga tgttcgggat ccatggttgt
     2281 ttcccacagc tgatgatcgt ccatttcacc tagcccctta taacgctgaa tagtcggttt
     2341 ggtacgacct tcactataac gggctaaagc ttcttggagt ttgatttctt gatctgcacc
     2401 cggctggata tattctttaa tctcgcttcc aaccttgaca ccatagattg gtggttgggc
     2461 aatataaaca taaccagctt ctaggattgg tttcatataa cgataaatca aggttaaaag
     2521 aagggtacga atgtgggctc catcgacatc ggcatcggtc atcaaaacga gtttttggta
     2581 acgggctttc gaaacatcaa attctgcgcc aaatcctgtt cccatggctg tgaaaagact
     2641 acgaatttct tcgttagcta gaatcttatc catacttgct ttttcaacgt tcaaaatctt
     2701 accgcgaatt ggaaggatag cctgaaactc acggttacga ccagatttgg ctgatccacc
     2761 agctgagtct ccttcgacga tgaagagttc tgtttcagca gggttattag aagaacagtc
     2821 tgctagtttc cctggaaggt tggaaatttc caaaccagat tttttacgtg tgacttcacg
     2881 cgcacgcttg gcagccacac gagccttggc agctaaaatc cctttttcca cgatacgttt
     2941 ggcaatctgt ggattttcca tgaggaaatc ggagaaggct tcactgaaga ggcgattggt
     3001 aatcttgacc acttcgctat ttcccaattt ggtcttggtt tgtccttcaa actgtggatt
     3061 tgggtgttta actgagataa ctgcagttaa tccttcgcga acatcttccc ctgttaaatt
     3121 atcttcattg tctttcagta acttattctt acgagcataa tcattgataa cacgagtcag
     3181 tgccgtacgg aaaccttgtt catgcgttcc accttcatgg gtatgaatat tattggcgaa
     3241 actcatgaca ttttcatggt aacctgttgt gtactgcatg gctacctcaa ctgtgatatc
     3301 atccatctca ccgtctgtat agattggtgt atcaaagatt acatccttgt tctcgttgat
     3361 atattcaacg taactagcaa tcccaccttc ataatgataa tgcttggttt gttccaaacc
     3421 ttggcgctta tctgtaattg aaatttgaag accgcgattt agaaaggcca actcttgaat
     3481 ccgtttattt aatttatcaa aatcaaagat tgttgtttca gtgaaaattt ctgggtccgg
     3541 tgtgaagtga accgttgttc cagttctatc tgtatcccca accacctcaa ggtctgcaac
     3601 aacatgacca cgacggtatt cttggtaatg aatcttaccg tttttatgga catgaacgtc
     3661 taattgagtg gaaagggcat taactactga cgaccccacc ccgtgaagac cacctgaaac
     3721 cttgtatcca ccaccgccga actttcctcc agcatgaagg actgtaaaga cggtctcaac
     3781 agcaggacgg cctgtttttt cctgaatatc gactgggata ccacgcccat catccacaac
     3841 agtaatcgaa tcatctggct caataaaaac ttgaatatgg ctggcaaatc ctgccaaggc
     3901 ctcgtcaatt gagttatcaa caatttccca gactagatgg tgaagacctt cttttgaggt
     3961 tgatccaatg tacatccctg gacgcatacg aacagcctct aagccctcta aaacttgaat
     4021 ttgactggca tcataatcct gtgcctgcag atttttgatt tcttctgtca tcttattcct
     4081 ttttcttata tgtctaatac ttgtcttaaa ttcacgatac tggtaaacaa gtgggtatca
     4141 agtcctgcag cttgacctgc ttcgatatca atcggccgat caccaatgac aagaccagag
     4201 ctaatctgat acttttctct taaataaagc atggattcgg gatttggctt tctcttaaag
     4261 cctgagctag aagtcaccac ttctgtaaaa taagctgcta tagaggtttt ttctaaaatt
     4321 tccaaaacct gatcatttcg atgagagacc aaaaaatgac ggccaccttg atttaaaatg
     4381 tcttccaata ggtcagaaac tccttcaaat aaaatcgggt gttcaagctc tctggcttca
     4441 ttttccttgt acttttctaa aaaattctct aaattgggag cgaatgtctc aatcgcaaaa
     4501 ggagtagaaa cctttaaagc ttgatagaca ctgtcatggt cttgtgtgat accatacagt
     4561 gccaatgttt caacaaatgc agctgttgaa gtttcataat tatccagtaa agttccacct
     4621 aaatcccaga tgtaatcgtg atatttcata cccttcatta tagcattttt ttatgaaaaa
     4681 agcgatgatt tccctgagtt ttccacataa aaaacgagag aataactccc gttttactga
     4741 tttttaccaa agacatctcg atagagcatt cctgttcgta aactctctgc gtcccctcct
     4801 agttgctcca ccaactcgta ggcaaaggca agggctgttg aaggaccccg actggttgtc
     4861 aactgaccat ctaccactac tgtttccttg acgtagtgac catcaaggat ttgctcttga
     4921 acgccgtcat aacaagtgta tcgcttattt ttcaatatct ctgcttgatt gagggcaatt
     4981 ggtgccgcac aaatggctgc tagtttcttc ccttcttgct cgaagctttg caattcttga
     5041 atcaaggtct gattatcacg taaatgtgca gaaccaggca tacctccagg aagaacaatc
     5101 atatcatagt ctgataaatc tccatcaaag acatgatctg ctcttacttg gattgcatgc
     5161 gaacccgtta cttgctcttc aaaaccaacc atatcacatg tgatattggc tcgacgcaag
     5221 acatctacaa ctgtcaaggc ttcaatttct tcaaagccct gagctaacat aactgctact
     5281 tttaccatga ttttctcctt atttaatttg ttttaataca acctttttcc gtttgaatgg
     5341 gacttttccg ctcttttctt cagctaaatt agtctggtaa ctcatcgata aaagcaaacc
     5401 aacaccaatc aaattactga taatagctga tcccccttgc gaaatgaaag gcaagggaat
     5461 ccccgtcaaa ggaagtagtc cagtcacagc accgatattc tcaaagatgt ggaagagcaa
     5521 catcataatc aaacctgtgg aaatataagt gtagaactgg ttatttgatt taagagtaat
     5581 cttcaacata cggtaaatca acatgagata gagggcaata accaggacag agccaataaa
     5641 gccaaaatct tctgcaataa ccgtaaaaat catatctgac tctcgaactg ggataagcag
     5701 attcgaagca ttaaatccct gaccaaataa gccaccactc ccaatggcaa tctgcccttg
     5761 agcctgctgg taagtcgttg tttgggcaaa ctcaaaggga ttgagccaag ccaaaatccg
     5821 attaatttgg taggtcggca ttccaatctg gtgaagaaaa gctcgtccgt ccttgctaat
     5881 aaagatagct aagaaaccag caactcctgt tacagcagtc acaaatactg ggataataat
     5941 tttccaagaa acccctgata ataaaacgat tcctgagaaa atggctacaa aaaccaaagc
     6001 cgtccccaag tcactttgaa gtgctaaaag aactaggact ggaatggtaa agagaatcat
     6061 ccagaaaatt aacaaaaagt ccagcggaac cgtgcgtctc cattccttat gtttctttgt
     6121 aaattggaca atgacacgag ccaacatgag gatataggat atcttcataa attctgacgg
     6181 ttgaaatagg gtaattccat ttattgatac ccagtttttg gcacccgttg atgcaactaa
     6241 gcttggatta taaaatacaa tcggcaagat cataagtccc aagcctaaaa tatatagaaa
     6301 gggggtcacc ttccaaagaa attctgtatt aaagagcatg accacaaaac caatcacaag
     6361 ccccaaggca atccaggcga cctgctgccc taaaatgggc agaatattat tgggataatc
     6421 atgactaacg gctatataga tagccaccac accgatgacc agtagaaaaa atactggcaa
     6481 gagcaaacta taatcgactc tagagtcgag agaacgtttc atatgtacta accttatact
     6541 ttcatacaat actatttatc aaagttcatt aaaaaatcta tcaatagcct cgtcaacttc
     6601 ggatcgagag atggttttaa cagtcgcttc ttctgctaga gatgctacta tttgtttgcc
     6661 gtatcgtttt ccgacgattc tcctatccaa aataagagtt aaggaacgtt ggtattcacg
     6721 tctcatactt cttcccaaag cctgttttaa acgaataatg gccattggca attgataatc
     6781 ataaaaggca tttttccctt cttgattcag ttcttgatta atctttttcg tcaagggttc
     6841 ttgaggattt tggaaaggaa gcctcggtac aacttgaatc acagaaggat ggcttgaaaa
     6901 atcaactccc tcccagaaac ttgctgcacc aagcaagatt tgttgttcac ctttttcaaa
     6961 gcgtttcttt agctgatgaa catccccatt tttatactgg gccaagtggc taactgtaag
     7021 taaatccgat actgctagaa gcatgtcttt agcggtaaag agaaccaaaa tcggttgctg
     7081 gaaagcttga atttccacta gtaaagcagc tacctctctg gcatagactt ctaaggaggt
     7141 ttctgttacc agagggaaat ctttgaccat aaccacttct tgttcctgtt ttccccgaga
     7201 ttcaatcttg acaaatttgg cttcaggata gcctaaaagg tctgccaaag aaaccctctg
     7261 actaatctca agagtagccg atactcccaa gacttggcaa ctctcaggaa gtaaagagga
     7321 cagaagagta cggcctgatt ttgtagaaga aatctgaatt ttcttttgac tcgtttcagt
     7381 tacttcaagc cagtaatcac cttcagctgt aaaatagcga accagctcat caaagccttc
     7441 cacttctaat tctgaaaaat actgatggag attgtccaga gaatctaaga tatttttcct
     7501 agatttacca gactgaaatt gttctatcaa gtagagacac tcaaagcgaa tactttctag
     7561 tatccgttgt tgaaccctgt tttcttctcc tactaaagcc ttatcaatta aatcgataat
     7621 agattgtata tcgtaggtct cttgaagcag attttctaga gctaacaaaa tcttttggac
     7681 ttcatcaata atcagtaaac ggtcactgac aaattcagga ttatcttcaa gtctggtcac
     7741 gagataggca tgattagtca ctaaaagctt gcaagtctct gccctttctt gactacgttt
     7801 ccaaaaatct tccgtcacaa ataagctctg ggatgataaa ttcccatcat gacgaaggtc
     7861 tgctagaaaa tgttggtaac ggtagagttg cccgatttca tccaaatctc ctgtctctgt
     7921 ctcagtaagc cagaccaaga cttgcatttt aaagcgtcta aataagcgat tttcatcatt
     7981 ttcctgcaag gaatgataaa aggcatccaa cttcagataa ttttgtggtc cctttaagct
     8041 atgaatatct gtatggaaca cttccttgag gcgtttacct tcttcttcca tgatttgatt
     8101 ttgaagaatc tttgtcggaa cactaagaac aatttgtcgc tctttggatt gagataaagc
     8161 gggtaagaga tagccatagg ttttcccaat ccctgtcggc gcttgaatca gagagacagg
     8221 ttcatctttc aatagcaagc caacctcttt agcaaaactt tcttgttcct ccctcacttc
     8281 aaggttcaac agagaaatat ttttagaaaa gtcttgagat agttttcgta gctccagaga
     8341 agctgccgtt ttcttaaaat acagaccttg aacttggacc aagtctggag aactcaggat
     8401 agattggttg cgataagttt cctcaataac caggtaggac tcatatagga gagcgtcagc
     8461 catttccagc aagcgttcca agagaccttt aggaagctgg gtcatctttt cccgtaaaaa
     8521 aagaagtaat tctgctgtag cttgggcatc tgaaagggct gtgtgtgcgt gtttaagagg
     8581 aattcctaat tctcgacaca aaatcggcaa gctatatttt tccagttcag ggaaaaagac
     8641 ctgggccaat tcgaccgtat caacacgagg gtttcttagc tcatagcctt caaaaaataa
     8701 attttccgcc aagagattag catcaaactg aacattatgg gctacaaaaa tcccatcctc
     8761 caccaagtca aatatttttc tggcaacttg cgaaaaatca ggtgcttgcg ccagacgttg
     8821 gtctgtcaat cctgtcagtt ctttgatatg agcatccaag ggttcatgtg gattgacatc
     8881 cgtcgtatag tgatcgacga tttctccatc ctcaatcacg acaattccca cttggataat
     8941 tttagcctta ctacctgtgc tagttgcctc taaatccacc acaacatagc gattaccaat
     9001 cgttttcatt ataaaaaatt ataccaaaaa aagggccatt tggcacctgc aaacatagga
     9061 taattttcaa actcaagaac actacttttc ttaaaaatcc catcgaatag agcattctag
     9121 cctgctgatt tttgtaaaat agtgaagaaa tacgaaaaat tgagagaaga aatgtttgca
     9181 tcttccttct ctcaacgaat catgtatttt atctaaccat gtcaggatcg tagtcataaa
     9241 atcctgtatc aaggaaacgg tttttagggt gaagtttatg aacttcttca tccagcaaga
     9301 aggcttgatc atggctaaag ttgccctctt gaatcaaatt acgcgcttgg ataaagagtt
     9361 ttgcaatctc aacaaagttc tgaccaggag tgtagagccc ttctagtttc cagcgagtaa
     9421 agccatgctc caccaattct gttaatttga tcatcaaatc aaggtcattg ttggcaaaga
     9481 tatgggtccc atgattatct tcaaaaatgg aatagtggct ctctggatca cttggctcag
     9541 ccaagaagag gtcacgttta tgcgtctttt catcatcgat atgtgtaaag ttatagtagt
     9601 tttgcaagag tggacgttta gaatgatgga tgacgctagc accgtaaacc aaaacttcag
     9661 caggaatttc caaaatctct ggcattttga aaagttcagc tgatggaatt tcacgcgcca
     9721 aaacagcctc agatgcgcca gccttttgtc cccagaagtt aatctgacga ctgctagtta
     9781 ccatggttga agcatcgtag atggtcttaa atgaataacc atcgcggtta actacgtaaa
     9841 agacgcctgc atccccaatc gtaatatagt ctgtcttgat ttcttccaag aagtctaaga
     9901 aaggcttgat acggtccatc atatcttggt gcatgagagc attgaccgca acgatcaatt
     9961 ccttaccagc atcatgaacc aacttagcga tttcacgtaa ttggtcataa ctaaaggtcg
    10021 ttggcagacg aagaccaaaa tctttctcac cgacatagat acggtctacg ccagcttcga
    10081 gtagttgttc aacttgttca atactttcag cagttgctgt aatgacaatc ttttccatag
    10141 gaaaaattat accacatttc tccaaaatca gccatgcttt gaaaatgaaa agggacttta
    10201 ttctttttgt aacctttctg taataattct ctgccgaaaa aatgataaaa taggtatgta
    10261 ctgttaagga gagaatcatg cccgtaagaa aattacaatc ctatgaggta gactatcaag
    10321 aagaattaaa ccagcagctt cctcattacc aagcttatac acctgaagcg caatctgatg
    10381 ccaatctcaa ggaaatccta ttttttatta atatcgctgt tttttgtatc tgtattgcta
    10441 tctttagttt tatcttttta gcattaaaat tatcaactgc tcttgccttt gccgcagcaa
    10501 tcggattcag cttacttgtt ttaaaagttc aacggtctat tatcaaacga aaacgtagaa
    10561 gataaatcca aaatatcgcc ccaattggag cgatattttt attttttctt tttagatggt
    10621 gtctgaatgg caatcttcac ttcaaagatt gttcccttgg gtttattatc tttgacagta
    10681 acagttcctt ttagagcatc tacaatttgc ttggctaggg ataatcctaa accaaaacca
    10741 cctttttgcc gggttctagc tttgtctact cgataaaaac ggtcaaaaat tttcttttta
    10801 tcttctgtcg aaataccgat tccattatca gaaacaagta aataaagatt gcgatcggtc
    10861 gccgagataa gaaaatcaat ttcaccatcc tcctcagtat acttgacggc attatcgaaa
    10921 agaatggtca tcagttgttt cagaagaagc tgatctgtga caattgttcg atggatacga
    10981 ttttcaaaac ggaagacacg attattttcc gaagcaatca tctcatagtt tgtgaaagtt
    11041 gtattaaaaa agctagttgg aacttctgca agctccggct taatcccatc atctctccga
    11101 gctaagttca gcaagcttgt cgttaaaaaa cgcatatttc ggacttcttc caaactcgat
    11161 gcaatgcttt cgctcacatc cataatggta gcttctggct tacgaaaaag ggtctctaag
    11221 cgattttgca aaactgcgag tggagttcgt aactcatgac tggcattttc cacaaaagac
    11281 tgttgcttct gcatactctc aagcaggggc ctaacactga ccctagctag atagagactg
    11341 gcaagtaaag acaaaatcca gaaactagcc atcacgacca caatcaattg ctcatgcttt
    11401 tgactagcct gctccaactg actggtatta atcaagacag cagcatactt gatattggtt
    11461 gaaaccgaac taatattggt ctccatcaaa atcacacgat agatttcttc ctgcccatag
    11521 ctattaaaaa cctgaatctg gtagatatgt cctagttctt tcttttctaa cttaatctta
    11581 tccaagccca aaaatcgatt tccagaaaga agttgagtaa agtctttatc aaagagaatg
    11641 acttccgtat tggaactgac attaggtttt atttctactt tactagcgtc cgccctagca
    11701 ttttctaaat ctttaatctc ttctgttgcc ctatttatag ccagctgaat aactgcttga
    11761 ggattttcac tcagtccatg aagcttatcg tccaccgaag tataaagact cgaatgcatg
    11821 acttgtaaaa taatcagagt cattgtagaa aaaatcaggg tgaagacacc gaagttgcgg
    11881 ataaaataac taaagtcatc cgcataccat gtttttttaa gtttactgaa catcttttaa
    11941 aagataccca acactacgca aggtttgcaa attctctgca aaagtggttc cctttaattt
    12001 cttacggact tttgaaacat agacttcgac aaccgaaatc gttgtatcac tatcaaatcc
    12061 ccatagacgg tcaaaaatct gcgtcttagg caaaatcaca ttttgatttt gaaggaaata
    12121 aactagtaaa tcgaactctt tccccagcaa ttcgacagga gtatcttcaa ctttaacggt
    12181 attggttgat aaattaacca cgatattccc ataagtcaag gtgttttcat taaacttccc
    12241 tgaacgtttg agaagggcct gaattcgcat tttaagttct tctaggtaga aaggtttggt
    12301 cagataatca tccgctccca gttcaaatcc atgtcccttg tcatccaaac tttccttggc
    12361 agtcataatc agaactggtg tcgtaattcc cttttcacgc aattctttta agacttggaa
    12421 accatttttt tctggcaaca tcaaatccag caaaatcaag tcatagacac cactctcagc
    12481 ttcgtagaga ccttcttctc catcaaatac ctgcataaca tccgcaaaat cgtctaaaaa
    12541 gtcaaatact gaatttgaca gacctaggtc atcctcaacc aataagattt ttatcatgag
    12601 aaactcctcc ttattaaaac tattatacca aatttgcctt aaaaaaaact caactctctg
    12661 cattttacat gagatagctg agttttcttt ggctctataa tatttgtagt gggtaaatcc
    12721 actatggata ttatggagcc tattttattg tagaaaaaaa gtcccatatg acctataatg
    12781 aaaagcgaca aaacaactca ttagaaagat tcatatggaa caattacatt ttatcacaaa
    12841 actgctcgat attaaagacc caaacatcaa gattctagat atcatcaata tggataccca
    12901 caaagaaatt atcgctaagc tggattatga ggctccatct tgccctgatt gtggaagtct
    12961 aatgaagaaa tatgactttc aaaaaccgtc taagatccct tacctcgaaa caactggtat
    13021 gccttctaga attctcctta gaaaacgccg tttcaagtgc tatcactgtt caaaaatgat
    13081 ggttgctgag actcccctgg taaagaa
//
LOCUS       DAVMGZ010000046        12879 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000046 DAVMGZ010000000
VERSION     DAVMGZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12879
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.047"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            131..868
                     /locus_tag="VYQ93_001080"
     CDS             131..868
                     /locus_tag="VYQ93_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3335779.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            865..2556
                     /locus_tag="VYQ93_001081"
     CDS             865..2556
                     /locus_tag="VYQ93_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3335780.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            2672..3235
                     /locus_tag="VYQ93_001082"
     CDS             2672..3235
                     /locus_tag="VYQ93_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV3335781.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            3257..8926
                     /locus_tag="VYQ93_001083"
     CDS             3257..8926
                     /locus_tag="VYQ93_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEV3335782.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIF
                     DLEYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYL
                     EGRDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADI
                     EVHRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPY
                     GAIDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRIS
                     EEEANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTK
                     FTPYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGS
                     QKRYKVTSTGKFSITNLPEYQVEDLNVMYTSEHIVHPLDSSLINNLVEELKKVELYTE
                     STYQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAV
                     AALRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRL
                     GSSGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDY
                     IQFEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSR
                     EKRPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGS
                     EWVDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSE
                     YGISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAF
                     ERKNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFK
                     KVEPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFET
                     SYIAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRD
                     EGHAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEE
                     LQEGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            8987..10708
                     /gene="pabB"
                     /locus_tag="VYQ93_001084"
     CDS             8987..10708
                     /gene="pabB"
                     /locus_tag="VYQ93_001084"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV3335783.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            10828..12354
                     /locus_tag="VYQ93_001085"
     CDS             10828..12354
                     /locus_tag="VYQ93_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV3335784.1"
                     /translation="MKSKIVLGASLAIATLSLVSLVEIEGLSPFLIENVSANTHSANK
                     VINHKVSIYLENADEGKGLTVNFSTDSVSPNLFDEFEKKSGITITTMLVNAKTGEVVE
                     KRLTPSVFLRSNDLTSGTISSFIFSEYPDGEYKYVVSKGDFIDPKTQFKHQYRGESPV
                     FRIRNRKYVELGTTDKKLDERRDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSV
                     NSNLLNSFGEKAKKVLIRSKLINVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDL
                     TDGEYKYVAYESQQYTDPQTTLTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPK
                     PDEKPKPSAPQQEKTKPTVQSGWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYV
                     QNAWVGNYYLKSGGYMAKSEWIYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAK
                     SEWVYDSNYKSYYYLTSEGSYARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK
                     "
     gene            12441..>12879
                     /locus_tag="VYQ93_001086"
     CDS             12441..>12879
                     /locus_tag="VYQ93_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV3335785.1"
                     /translation="MSTIDKSGVLPFSAQTVQADIQTLTPMAKYYIILPKDAEIYKTW
                     RGTVNIPIIDATKTTPELSYFKEDHRNYIANENKSGANYIEWKGTVEEFKEAIKKLTD
                     KKSTTATPKKDEKPTPKPDEKPKPTPTVQSGWVGSSYYQDGKKV"
BASE COUNT         4337 a         2117 c         2801 g         3624 t
ORIGIN      
        1 gtactttgag aaacctgtgg ctatcttcct agtttacttt ttgattttta ttgagtataa
       61 gaggtctttc ctaagcagtt agaggagaat tttgctatac tgatactagt aagtggtaaa
      121 ggagcagagc atgacctaca caatcttaat cgtagaagat gaatatctgg taagacaagg
      181 tttgactaaa ctggtcaatg tagcagccta cgatatggaa atcatcggtc aggctgaaaa
      241 tggaaggcag gcttgggaat tgatccaaaa gcaggtgcca gatatcattt taaccgatat
      301 caacatgcct catctaaatg gcatccagtt ggccagtctg gtacgagaaa cctatcctca
      361 ggttcatttg gtctttttaa caggttacga tgattttgat tatgccttgt ctgctgtcaa
      421 actaggtgtg gacgactacc tgctcaaacc cttttctcgt caggatattg aggaaatgtt
      481 ggggaaaatc aaacaaaaac tagacaagga agagaaagaa gagcagttac aagatttatt
      541 gactaatagg tttgaaggaa acatggccca gaaaatccag tctcatctgg ctgatagcca
      601 atttagttta aagtctttag ccagtgactt aggttttagt ccgacctatc tgagttcctt
      661 gattaagaaa gagttgggct tgccttttca ggattatctg gtgagagaac gtgttaaaca
      721 agccaagctc ttgcttttaa ctacagatct gaagatttat gagatcgcag agaaggttgg
      781 ttttgaagat atgaactatt ttacccaacg ttttaagcag attgcaggtg tgacacctcg
      841 tcagtttaag aagggagaag accgatgaag cgttcttctc ttttagttag aatggttatt
      901 tccatctttc tggtctttct cattctccta gctctggttg gaactttcta ctatcaatca
      961 agttcttcag ccattgaggc caccattgag ggcaacagcc aaacgaccat cagccagact
     1021 agccacttta ttcagtctta tatcaaaaaa ctagaaacca cctcgactgg tttgacccag
     1081 cagacggatg ttctggccta tgctgagaat cccagtcaag acaaggtcga gggaatccga
     1141 gatttgtttt tgaccatctt gaagtcagat aaggacttga aaactgttgt gctggtgacc
     1201 aaatctggtc aggtcatttc tacagatgac agtgtgcaga tgaaaacttc ctctgatatg
     1261 atggctgagg attggtacca aaaggccatt catcagggag ctatgcctgt tttgactcca
     1321 gctcgtaaat cagatagtca gtgggtcatt tctgtcactc aagaacttgt tgatgcaaag
     1381 ggagccaatc ttggtgtgct tcgtttggat atttcttatg aaactctgga agcctatctc
     1441 aatcaactcc agttggggca gcagggcttt gccttcatta tcaatgaaaa ccatgaattt
     1501 gtctaccatc ctcaacacac agtttatagt tcgtctagca aaatggaggc tatgaaaccc
     1561 tacatcgata caggtcaggg ttatactcct ggtcacaaat cctacgtcag tcaagagaag
     1621 attgcaggaa ctgattggac ggtgcttggc gtgtcatcat tggaaaagtt agaccaggtt
     1681 cggagtcagc tcttgtggac cttgcttggg gccagtgtca catctcttct tgtctgtctc
     1741 tgcttagtgt ggttcagtct taaacgctgg attgctcctt tgaaggattt gagagaaacc
     1801 atgttggaaa ttgcttctgg tgctcaaaat cttcgtgcca aggaagttgg tgcctatgaa
     1861 ctgagagaag taactcgcca atttaatgct atgttggatc agattgatca gttgatggta
     1921 gctattcgta gccaggaaga aacgacccgt cagtaccaac ttcaagccct ttcgagccag
     1981 attaatccac atttcctcta taacactttg gacaccatca tctggatggc tgaatttcat
     2041 gatagtcagc gagtggtgca ggtgaccaag tccttggcaa cctatttccg cttggcgctc
     2101 aatcaaggca aggacttgat ttgtctctct gacgaaatca atcatgtccg ccagtatctc
     2161 tttatccaga aacaacgcta tggagataag ctggaatacg aaattaatga aaatgttgcc
     2221 tttgataatt tagtcttacc caagctggtc ctacaacccc ttgtagaaaa tgctctttac
     2281 catggcatta aggaaaagga aggtcagggc catattaaac tttctgtcca gaaacaggat
     2341 tcgggattgg tcatccgtat tgaggatgat ggcgttggct tccaagatgc tggtgatagt
     2401 agtcaaagtc aactcaaacg tgggggagtt ggtcttcaaa atgtcgatca acggctcaaa
     2461 cttcattttg gagccaatta ccatatgaag attgattcta gaccccaaaa agggacgaaa
     2521 gttgaaatat atataaatag aatagaaact agctaactcc cagtcaattc tgggagtttt
     2581 atgcgtaatt gggcaaactt tctaggttgt ctatcttgct aaaaacaaga aaaaacggta
     2641 tgatagaaga gtgacaacag aggtaacaga gatgaacgag acacagattc aaagagaaac
     2701 acgtcaggta gtggaagatg ttttagaaaa gaccaatttg aagcaggggg ccctttttgt
     2761 tttaggtcta tcttctagtg aggtgctagg tggtcatatt gggaaagaat ccagtcaaga
     2821 gattggtgag ctgatagtgg agactatctt ggatattcta ggtagtagag gtatccattt
     2881 agcagttcaa ggttgtgagc atgtcaatcg ggctctcgtt gttgaacgtc aggtggcaga
     2941 gcagtttggt ctggaaattg tcagtgtcca tcctactctt catgcaggag gttcggggca
     3001 gttggcagcc tttaagttta tgcaggatcc agttgaggtt gaatttatca aggctcatgc
     3061 tggattggat atcggagaca ctgcaattgg catgcatgtc aagcatgttc aggttccgat
     3121 tcgccctatt ttgagagaga ttggtcatgc ccatgtaacg gcactggcta gtcgtccaaa
     3181 attaatcgga ggtgcgcgtg cgcactatcc gcaagatgct attagaaaga cctgagaatg
     3241 aataaaggag ataaaagtgt ttaaaaaaga ccgtttttca attcgtaaga ttaagggagt
     3301 tgtaggctct gtatttcttg gaagcctttt gatggctcct tctgtagtgg acgcagccac
     3361 ctatcactat gtaaataaag agattatttc acaagaagct aaagatttaa ttcagacagg
     3421 aaagcctgac aggaatgaag ttgtatatgg tttggtgtat caaaaagatc agttgcctca
     3481 aacagggaca gaagcatctg ttttgacagc ttttggttat ctatcaggtg acatcctcaa
     3541 aacgcttggc ttggacactg ttttagaaga aacctcagca aaacctggag aggtgactgt
     3601 agtcgaagtt gagactcctc aatcaacaac aaatcaggag caagctagga cagaaaacca
     3661 agtagtagag acagaggaag ctccaaaaga agaagcacct aaaacagaag aaagtccaaa
     3721 ggaagaacca aaatcggaga taaaacctac tgacgacacc cttcctaaag tagaagaggg
     3781 gaaagaagat tcagcagaac cagctccagt tgaagaagta ggtggagaag ttgagtcaaa
     3841 accagaggaa aaagtagcag ttaagccaga aagtcaacca tcagacaaac caacagagga
     3901 accaaaagtt gaacaagtag gtgaaccagt cgagccaagt gaagacgaac aggcaccaac
     3961 ggcaccagtt gagccagaaa agcaaccaga agctcctgaa gaagagaagg ctgtagagga
     4021 aacaccgaaa ccagaagata aaataaaggg tattggtact aaagaaccag ttgataaaag
     4081 tgagttaaat aatcaaattg ataaagctag ttcagtttct cctactgatt attctacagc
     4141 aagttacaat gatcttggac ctgttttaga aactgcaaaa ggtgtctatg cttcagagcc
     4201 tgtaaaacag cctgaggtaa atagtgagac aaataaactt aaaacggcta ttgacgctct
     4261 aaacgttgat aaaactgaat taaacaatac gattgcagat gcaaaaacaa aggtaaaaga
     4321 acattacagt gatagaagtt ggcaaaacct ccaaactggg gttacagaag ctgaaaaagt
     4381 tgcagctaat acagatgcta aacaaagtga agtaaattct gagactgcaa gtcttaaaac
     4441 agcaatttct agattaaata cagataaagt tgaactcgaa aatcaactta agattgcaca
     4501 gggaaaaact gaaactgatt tcagcatgga atcttggact gttttgagta cagctaaaaa
     4561 taaagctcag gaagtaaaag ataacggaac agcgacccaa gaacaaataa acgaagcaga
     4621 gaaaagtttg aaaacagccc ttgctgactt atctgttgac aaaacagctt tgggaagcgc
     4681 tatcgataca gctactaaga aaaataaaga gaattataca aatcaaacat gggcagagtt
     4741 ggaaacagcg cttacagcag caaaatctgt aaatactaac gagtctaaac aaagcgacgt
     4801 taacgaagct gctgaaaaat taactgcaac tatggaaaaa ttggttgaat tgtctgaaaa
     4861 gccacgtttg actctatcta ttgaaaagag agacatagat cgaaaagcta cggtcacgta
     4921 tactttggaa aatccagcaa atactcaaat aaaatcaata actgcaactc tgaaaaaagg
     4981 agaggaagtt gttaaggatt ttgttttaac agaagaaaat cttaaaacta atcatttaac
     5041 tgctttgttt gaaaagctag actactacaa agaatacact ctatctacgg atatggtata
     5101 taacagagga aatgatgatg agacggagag tatttcagaa gaactaattc agctgaatct
     5161 taagaaacta gaattgaaag atatccagac agtctctctg atgaaatttg aaaatggtca
     5221 agaaagtcag gttacacact tgtctgataa accgaccgat ttgagtaaat tatacttaaa
     5281 agttacatca tcaacgtcta aggatgctgt gttagccgtt agctctatag aagaagagat
     5341 tattgaaaat aaaaaaatct ttaaaattca tgcagataca ccagaattag tagttcgtaa
     5401 gaaagatggc agtctttcta agggatttga ctattacatg gagagagtta tccctcatga
     5461 tggtgatatt tattatgatt ttaaagactt gatttcagct atgacatcta atcctacagg
     5521 tacgtttatt ttagggagag atatttcctc gcgtaatgta aaaccggatg gaaatggaaa
     5581 atcttatatc aaaggtgaat ttaaaggaaa actacttggt acaaatgata atgttagaca
     5641 ctcgatcttt gacttagaat atccattgtt tgatactata aaatcaggag tcgtgaaaga
     5701 tattgatttc aagcatgtca atatggtatt cccagatagc aatcaaggag ataacgtagc
     5761 aacaattgca agagtaatca aagataagac aaaaattgag aatgttaatg ttgaaggtta
     5821 tctcgagggt cgagatcatg tagctggttt agttaataat cttgaaggta attccgaaat
     5881 tgaaaatgta tcctttacag gaaagattaa gtctaaaggt ggtaattcta ttacagcagg
     5941 tattgcaggt agaaatatct tatctcgtgt taaacgtgcc tatgtaaatg cagatataga
     6001 ggtacatcga tcatctaatt ctagcatgct tgttgcagta aatggaataa acgcagatgc
     6061 aagtggtggt tggggaacct ggggtagact tactgagtca gttgctaaag gaacattaga
     6121 aaccaagcaa ggagggcaag ctggaggagc ctcttctaca gtctggcctt atggagcaat
     6181 tgataatgtt gtcagctatg caaaagtgac caaagggaaa gaattatttg gatcagatgg
     6241 tgatttaaat tatgattggt ttatgaaaaa aattagtaat atttttggtg ttcaaggcat
     6301 cagctcaggc gattcgggta gtgattctaa atttacgcgt atttctgagg aagaagcaaa
     6361 tcaaaaagtt gcttcttata atattacagc tcccaacttg atgagtgact caagtctttt
     6421 ggtagacaga ctcaatgaaa gttggaaaaa tactgatcaa tttgaaagta tacaggatta
     6481 tcaagcccaa aaccaattaa tttaccagaa cttgactaaa tttactcctt attacaataa
     6541 agaatttatt gttcatgaag gaaatgctct cactcctgaa caggaaatat tgaagactaa
     6601 gaaaatcaaa tcgattgtag ggttgaaagg aacagagttc gttgtagatg gcagtgatat
     6661 tgatactatc atgcttcatt ttgaggatgg tagtcagaag cgatataagg taacctctac
     6721 tggtaagttt tcgataacta atttaccgga atatcaggtt gaagatttaa atgtgatgta
     6781 tacatcagaa catatagtac atccactaga ttcgtcactg ataaataacc ttgttgaaga
     6841 attgaaaaaa gtagaattgt atacagagtc tacttaccaa gttctaggta ttgataaaga
     6901 taatgctaac aaattgaata gaactaagag attattctta gatgagtcat tagatgctgt
     6961 taaaacacaa ttgcctacct ttgtaaaaac aatgtttgaa aatgagtggt tgcacatcaa
     7021 tggagaatct agtggagctg tcgctgcttt acgacaaaaa atcatggata ataagacggc
     7081 tatattgctt gctttgactt atatcaatcg ttattatgat gttaaattct cagactataa
     7141 tattaaaaaa ctgatgctct ttaaaccaac tttccatggt gaaaagattg atctattaga
     7201 tcgattaatc cgactaggat catctggaga aaatagatta aaaggttcag aaaatgcaga
     7261 aacgttcaag cagttatttg ctagcgaaac gaagcagaag gatttggtga cttatttaga
     7321 ctacaatcgc tctttgttga ctaattatca aacaacaggg gaatggttta aagaaacgac
     7381 taaggattat attcaatttg aggaacgtcc atccttagta gaggagatta aagatgccaa
     7441 atatcgagtt tacgataatt taaccgcacc gtattatcaa ggatatatcc tacctctctt
     7501 aacgttgaag aatactcatt tagcaatttt atcgaactat agtactatga catttgtcag
     7561 ccgtgaaaaa cgccctaatt ggaaaaatga agattttgat aagtgggtga aatatgtagc
     7621 aactgctcat agaaaccatg ttgatacttg gtataagatt ttacctgata atatcaaagg
     7681 taaaatggtt aaagagaatg ttactgctgt ttgggaagga ttgagcatcc caggaagtga
     7741 atgggtagac cagaatgctg tagatagaaa aggaagagat tacgccccag ctcgagaatt
     7801 ctttaatctt gtaggtggtc ctatgggagg ttggtatgct taccatggtt atggtgcgca
     7861 tgctggagga agaaatcggg ttaattatga ggtgtttgat gtattaagtg agtatggtat
     7921 atcggtcttt actcatgaat tgactcacgt taatgatact tggatttact taggagggta
     7981 tggtagacgt gaaaatatgg gacctgaagc ttatgctcag ggattattcc agtctcctgt
     8041 tccaggtcag ccgggttggg gggcgcttgg tctcaatatg gcatttgaaa gaaaaaatga
     8101 tggtgatcta atttataatg cctccccaac tcaatttgaa aataggaaag agttagatag
     8161 ttatatgaaa aattataacg atactctcat gatggtggac tacttggaag gagatgcagt
     8221 tatctctaaa ggtaaagaag ctattactaa atggtttaaa aaagttgaac ctaaggttgt
     8281 tagtcaaaca gctcagtatg atacggttcg tcagttgact gctgaagaga aagaaaaact
     8341 ttctgtttct agtgttgatg atttagttga tcaaggatta atgtcagaca gagctgtagg
     8401 aaataatacc tataatccag ctgattttga aacaagttat attgcgatag attatatgac
     8461 tggtatttat ggtggaggaa agaatagtgt aggttcacca ggtgccttga tgtttaaaca
     8521 taatactttc cgtatgtggg gctactatgg ttttgaagaa ggtgttctag gatatgcttc
     8581 taataaattt aagcaagcat ctagagatga aggtcatgct ggacttagtg ataactttat
     8641 tatttctaag atttccaaag gggaattttt aactatggaa gcatttaaaa agggatattt
     8701 taagaaagta gtagaagagt taaaaactaa aggtattcgt cctgttacta tcaatcaaaa
     8761 aacatatagc acctttgaag aattgcaaga aggatttaag caggctgttg aaagagattt
     8821 gaagaaaaat cagttagatg agagggaaac aagaaacttc aaattccaag ttttcagaca
     8881 acttcttcaa caaacagata gctttaaaac ttctatcttt agataatcga aatctctcat
     8941 ctgctttgcg gatgagggtt ttcttatttt atgctatact taaagtatgc atagaaaaac
     9001 agtgattgat tttaggactt tgggggagag atataccttt acccagccta ttaaagagtt
     9061 gaaaacgaga aatgtagaag aagtggcaga tttgctggca caagtggaaa gctaccaaga
     9121 gcaaggttat tatgtggtgg ggtatgtcag ctacgaggct gcacctgctt ttgaggaaaa
     9181 attagcagtt cacaaggatc ccctactggg agagtacctg ctttatttta ctgttcatga
     9241 tagcgtagaa acttccccta ttcctctgac ttatgaggat attgatttgc cctcaaattg
     9301 gcaggaagta acgtctgcag cagactatga aaaggccatt gcccaaattc accatcattt
     9361 acggcaggga gatacctatc aggtcaacta caccgtccaa cttaagcaaa agttaaatgc
     9421 caatcctttt gccatctaca atcgtatggt ggtagagcag gaggcgggct acaatgccta
     9481 tgtggaacat gacgagatgg cagtgatttc catgagccca gagctctttt ttgagcaaaa
     9541 tgatcgcgag ttgacaacac gaccaatgaa ggggacgact cagcgtgggg taactgacca
     9601 agaagatctt gaacaggcca gttggttgga acaggatccc aaaaatcgct ctgaaaatat
     9661 gatgattgtg gacctcttgc gcaatgatat gaaccgtatt tctgaagttg ggagcgagca
     9721 cgtggagcgt ctgtgtcagg tagagcagta ttcaactgtt tggcagatga cttcgaccat
     9781 caagagtcag ttgcgagagg atgtggacct tgttgaaatc ttccactcac tctttccttg
     9841 tggttccata acgggtgcac cgaaaattgc gacaatggag attatcaagg acttggagcc
     9901 tcaaccgcgt ggagtctact gtggaacgat tggtctcttg cttccaaatg gacgacggat
     9961 ttttaatgtg gccattcgta ccattcaact tcacaaaggt caagccattt atggagttgg
    10021 cggagggatt acttgggata gcacttggga atctgaatac cgtgaagttc atcaaaaggc
    10081 agcagttctc tatcgtaaac aagctcgctt ccaattaatt acaactggaa aaatcagcca
    10141 aaaacaactg atgtttgaag aacaacatct ggaaaggctg agaaaagcta gtcggtattt
    10201 tgcttatcct tttgacccag aaattttgag acaaaagatt gaagcagagt gtcagacttg
    10261 tgatgctaat caagattacc gcttgcgaat ttctctcagc aagtctggag agatagaatt
    10321 cagtcgtcaa atcttaacac ctcttagtcc aagtttttgt caggccaaac tttgtctgca
    10381 agaagcgaat ttgaatcaat cctttaccta ttttaaaacg acttacagac cgcatttgag
    10441 catagaggaa caagagaaga tttaccataa tggaaaagga gaacttcttg aaacctctat
    10501 cggaaatttg gttctgaaaa tcgatgggaa actctacaca ccgcctatcc gacttggaat
    10561 cttgccagga atttatcacc agtatttact agaaacagga caggtagaag agaaagtctt
    10621 gaccttggca gattttgccc aagcagaagc tatttacggc tgtaatgcag tgagaggctt
    10681 gtatgagttg caagtgatag gattgtgagt tttagctatt aatagttttt aatatatatt
    10741 aaaatatatt ggtgtttact tttgatgaga tatcatattt gtgttataat gtaaccgtaa
    10801 acaatagaaa tttaaaggag atttttgatg aaatcaaaaa tagttttggg agcttcgcta
    10861 gcgatcgcaa cacttagctt agtatctctt gtcgagattg aaggactctc accgttttta
    10921 atagagaatg ttagcgcaaa tactcattct gcaaataaag ttattaatca taaagtaagt
    10981 atttacttag aaaatgctga tgaaggtaaa ggtctaactg taaacttttc aacagacagt
    11041 gttagtccaa atttatttga tgagttcgaa aaaaagagtg gaattacaat aactactatg
    11101 ttagtgaatg ctaagacagg tgaagttgta gaaaaaagat taacaccaag cgttttttta
    11161 cgttccaatg atttaaccag tgggaccata tcttcgttta tttttagtga atatcctgat
    11221 ggagaatata aatatgttgt gtctaaagga gactttatcg atcctaagac tcagtttaaa
    11281 catcagtata gaggagaatc tccagttttt agaatacgta atcgaaaata tgtggagttg
    11341 ggaacaactg ataaaaaatt agatgaaaga cgtgataata gcgtttataa agatggggtt
    11401 gttgaacaca aggtaaattt aagtttaaca agttatcaag gaggtaatgg agttactgca
    11461 attttttcaa ctgatagtgt taactctaac ttacttaatt cttttggtga aaaggctaaa
    11521 aaagttttga ttaggtctaa gttaattaat gttaagactg gcgaggttat tgatgaaacg
    11581 ttttctccaa aagtaagttt aacctctaaa attttgaaaa gtggttctac agcagtattt
    11641 tattttatag atttaactga tggcgaatat aaatatgttg cgtatgaaag ccagcaatat
    11701 actgatcctc aaacaacatt gactcatcag tatagaggag aatctcctat ttttagtatc
    11761 aaagatggaa aattttctgg tttagtatct gcctcaaaac cagacgaaaa cccgaagcca
    11821 acgccaaaac cggatgagaa accaaaacct tcagcaccac aacaggagaa aacaaaacct
    11881 acagttcagt ctggttgggt aggttcatct tattatcaaa atggtaaaaa agtcactagt
    11941 aagtggattt ttgataaaaa atataattca tacttctatc tagatgcatc aggaaattat
    12001 gttcaaaacg cttgggtagg taactattat ctcaaatcag gcggatacat ggctaaaagt
    12061 gagtggattt atgataagaa ctatggttca tactactatc taacatcaga aggcagctac
    12121 gctcgtaaca cttggtcagg caattattat ttgaagtcaa atggtaagat ggctaaaagt
    12181 gagtgggtat acgatagtaa ctacaaatca tattactact tgacatcaga aggaagttat
    12241 gctcgtaaca cttgggtagg caattactat cttaaatcaa acggtaaaat ggctgttaat
    12301 gaacgtacac cagatggtta tcgtgtagat ggttcaggta aatgggttaa ataagaaact
    12361 aaaaaatagg ataataggta atatcagatg aataaaaaaa attattttca tgtgctttag
    12421 cattttcaac gattggaaca ttgtcaacga ttgataaatc aggagttctt cctttttcag
    12481 cgcaaacggt gcaggctgac atacaaacgc ttaccccaat ggcaaaatat tatattattc
    12541 ttccgaagga tgcagaaata tataaaactt ggagaggtac tgtcaatatt cccattattg
    12601 atgctacaaa aactactcca gaattatctt atttcaaaga agatcatcgc aactatatag
    12661 ctaatgagaa taagtctggt gccaattaca tagagtggaa gggaacagta gaagagttta
    12721 aagaggccat taagaagttg actgataaaa aatcaactac tgcaacacca aaaaaggatg
    12781 aaaagccaac accaaaaccg gatgagaagc caaaaccaac acctacagtt cagtctggtt
    12841 gggtaggttc atcctattat caagatggta aaaaagtca
//
LOCUS       DAVMGZ010000047        12654 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000047 DAVMGZ010000000
VERSION     DAVMGZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.048"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            99..491
                     /locus_tag="VYQ93_001087"
     CDS             99..491
                     /locus_tag="VYQ93_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335786.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            513..1304
                     /locus_tag="VYQ93_001088"
     CDS             513..1304
                     /locus_tag="VYQ93_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335787.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            1297..1938
                     /locus_tag="VYQ93_001089"
     CDS             1297..1938
                     /locus_tag="VYQ93_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335788.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLLVWFLGLLPLGIHFKTVALFFLLAQLMILYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            1942..2574
                     /locus_tag="VYQ93_001090"
     CDS             1942..2574
                     /locus_tag="VYQ93_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3335789.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDIEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            2602..2889
                     /locus_tag="VYQ93_001091"
     CDS             2602..2889
                     /locus_tag="VYQ93_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335790.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            3035..5548
                     /gene="secA"
                     /locus_tag="VYQ93_001092"
     CDS             3035..5548
                     /gene="secA"
                     /locus_tag="VYQ93_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV3335791.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            5629..6660
                     /locus_tag="VYQ93_001093"
     CDS             5629..6660
                     /locus_tag="VYQ93_001093"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV3335792.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            6662..7693
                     /locus_tag="VYQ93_001094"
     CDS             6662..7693
                     /locus_tag="VYQ93_001094"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV3335793.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            7732..8100
                     /locus_tag="VYQ93_001095"
     CDS             7732..8100
                     /locus_tag="VYQ93_001095"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV3335794.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            8090..9193
                     /locus_tag="VYQ93_001096"
     CDS             8090..9193
                     /locus_tag="VYQ93_001096"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV3335795.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFIEASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            9212..11227
                     /gene="recG"
                     /locus_tag="VYQ93_001097"
     CDS             9212..11227
                     /gene="recG"
                     /locus_tag="VYQ93_001097"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV3335796.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRIQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTAHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMLALHLEKKEHLD"
     gene            11543..12523
                     /locus_tag="VYQ93_001098"
     CDS             11543..12523
                     /locus_tag="VYQ93_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV3335797.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDHVYNWLCGSEIPFKYLK"
BASE COUNT         3700 a         2361 c         2883 g         3710 t
ORIGIN      
        1 ggaatttgta tgatatacca tcgtttagaa taagtctata tttaaaatag aagttataag
       61 gaatcttctt gaataaaaaa tgaaaggaaa cagaaaaaat gaaaattaag aaattattga
      121 aaatggttat tcctgtttta atgataagtg ctgttggaac tactttcgta gaagcaaatc
      181 agataggtgc ttttagtaat tttgttatta ctaccagtta taagagaaca ggttatttga
      241 ctaaagaaaa tgaaggtgcg gaatatatca tgaacttaaa tccttgtaga aatttacatc
      301 ccatgactgt taagcatcgt atagtaaatt ctaatggaga agctcgtagt ggagaatcgt
      361 taacaacctg tggtactcgt tcaactcatg gtaattgggc aacggttggc tatgtttatg
      421 cagcagatat ggcaagacaa aattggtggg atttatcagc tgccatttca ggtagttggt
      481 caccaaatta attcgtaaag taggtataat ttatgaagat tcctctctta acttttgcaa
      541 ggcataaatt tgtttatgtc ttgcttactt tgctttttct tgctttggtt tatcgtgatg
      601 ttttgatgac ttatttcttt tttgatattc atgcgcccga tctagctaaa ttcgatggac
      661 aagcaattaa aaatgactta ttaaaatcag cattagattt tcgtattctc cagttcaatc
      721 taggttttta tcaatcattt attattccaa tcatcattgt tttgctaggt tttcaatata
      781 ttgagctgaa aaataaagtt ttacgattga gtattggaag agaagtgagt tatcaagggt
      841 taaaaagaaa gttgactttg caagttgcaa gtatcccttg tttgatatat ttagtgactg
      901 tgctgataat tgcaattata acctatttct ttgggacttt ttctcctctt ggatggaatt
      961 ctctattttc tgatggaagt ggtttacaaa gactcctaga tggagagata aaaagctatt
     1021 tgttctttac ttgtgtccta ctaatcggta ttttcatcaa tgcaatctat tttttacaaa
     1081 tagttgatta tgtggggaat gtgactcgtt cggcaatcac ctatttgatg tttctttggc
     1141 ttggttctat gctgctctat agtgccttgc cttactatat ggttcctatg acgagtttga
     1201 tgcaagctag ctatggggat gtaagtttga tgaaactctt tactccttat atcctttata
     1261 ttgtccctta tatggtgctt gaaaaatatg aagataatgt ttaagaattt taacaatatt
     1321 tggctaaaga gaaagatcgt tttactactt cgtatagttc tgatgatgat tttgataaac
     1381 tatctattgt caacagcggt tcaaaagcag gatgctgtta tctttttcaa gagagaattg
     1441 atttcaatct tttcctataa tgactattct gaagcgaatt tagaaatccc caaactcttg
     1501 ttaaaccttt cgcttttcat ggtaggatgg ctctctgtca ttttacttga aagtgatttg
     1561 gcagaccatt accatcactt gattcgctat caatcaagct cctttttcga ttatacaagg
     1621 aaacgattgg ttgtcatttc taaatttttt actcaagatt tacttgtctg gttccttggt
     1681 ttacttccgc taggaattca tttcaaaaca gtcgcacttt tctttttact tgctcagtta
     1741 atgattttgt acttactact gtcttatctg atagcactga ttagtgcggg cgctggtttt
     1801 tccttttttc tctatttttt agcatttgtg ggacaagaat ggatgatgga tcatattgta
     1861 acagtgtatt tactactctt aagcttatta gttatgttga ttgttagtcg cttggaagag
     1921 aaatttaaga aaggataaac gatgagactt gaaattataa atggacagaa aatttatggg
     1981 aaaagaccta ttttaaatca gttgaatttg gtgtttcaat caggaaaaat ttatggactt
     2041 aaaggtgata atggatctgg caagacggtt cttttaaaga tacttgctgg ttatattaag
     2101 cttgacaaag gaaaagttct tcaagacggg aaagtctacg ggataaaaaa tcattatatt
     2161 caggatgcag gaattttaat tgaaaaagtc gagtttttat ctcatttatc cctgagagaa
     2221 aatttggaac tgttaaggta tttttcatct aaagttacgg aaaaaagaat tgcctattgg
     2281 attcaatact atgatttaca ggaatttgaa gacattgaat accgtcattt atccttagga
     2341 acaaagcaaa aaatggcctt gattcaagcc tttatttcct ctccttctat actctttctc
     2401 gatgaaccta tgaatgcttt ggatgagaag agtgtgaggt taaccaaaca ggtcatttta
     2461 tcttacctga aaaaagaaaa tggtctggtt atcctgacat cgcacatatc ggaagatatt
     2521 tcagaccttt gtacagatgt attagttgtc gaaaatggac atatacaaat gtaaaggata
     2581 tacaatccta ggagatggct tatggcacat ctaaaatcat ttattacacg ctattccaag
     2641 gtttatattg gtttagttct gctgatctgg ctgtctttct tctttatccc ttgggataga
     2701 gcgattctgg gggtaaggat tgacatcttc atcatacaga aaatcttgct agcttttgga
     2761 attctgtcca ttctcatggc cttgctgtcc aagaaagtca gtctctttgt ttttggactg
     2821 atttgctgtc tttctctttg gattaactta tttatcacat ttgccatttt gccgattttt
     2881 ggcaattaaa cagtcataaa agtcggagag gttagcttga aaactaacct ctttttcctt
     2941 ttcaaaatgg ggattcttcc ttgaaaataa tcagtaattg tgctaaaatt aaaggaacat
     3001 tctaaaatat tcggaattta aagtaaggaa aaacatggct aatattttaa aaacaattat
     3061 cgaaaatgat aaaggagaaa tccgtcgtct ggaaaagatg gctgacaagg ttttcaaata
     3121 cgaagaccaa atggctgctt tgactgacga ccaactaaaa gcaaaaacag ttgaatttaa
     3181 ggaacgttat caaaatggag aatcactgga ttcattgctt tacgaagcat ttgcggttgt
     3241 ccgtgaaggt gccaaacgtg tcctaggtct cttcccttat aaggttcagg tcatgggggg
     3301 gattgttctt caccatggtg acgtgccaga gatgcgtaca ggggaaggga aaaccttgac
     3361 tgcgaccatg ccggtatacc tcaatgccct ttcaggtaaa ggggttcacg tagttacggt
     3421 taatgaatac ctgtcagaac gtgacgcgac tgagatgggt gaattgtact cttggcttgg
     3481 tttgtcagta gggattaact tggctaccaa atctccaatg gagaaaaaag aagcctatga
     3541 gtgtgatatt acttactcaa ctaactcaga aatcggattt gactaccttc gtgacaacat
     3601 ggtcgttcgc gccgaaaaca tggtacaacg tccgcttaac tatgccttgg tcgatgaggt
     3661 tgactctatc ttgattgacg aggctcgtac acctttgatt gtatcaggtg ccaatgcggt
     3721 tgaaaccagt cagttgtatc acatggcaga ccactatgta aaatctttga acaaagatga
     3781 ctacatcatc gatgtgcagt ctaagactat tggtttgtct gattcaggga ttgacagggc
     3841 tgaaagctac ttcaaacttg aaaacctcta tgacatcgaa aacgtggctt tgactcactt
     3901 tatcgataac gcccttcgtg ccaactacat catgcttctc gatattgact atgtggtgag
     3961 cgaagagcaa gaaatcttga ttgtcgacca atttacaggt cgtaccatgg aaggtcgtcg
     4021 ttattctgat ggattgcacc aagctattga agccaaagaa ggtgtgccaa tccaggatga
     4081 aaccaagaca tctgcctcaa tcacgtacca aaacctcttc cgtatgtaca agaaattgtc
     4141 tggtatgacg ggtacaggta agactgagga agaagaattc cgtgaaatct acaacattcg
     4201 tgttattcca atcccaacaa accgtcctgt tcaacgtatt gaccactcag accttcttta
     4261 tgcaagtatc gaatctaagt ttaaagcggt tgtcgaagac gttaaggctc gttaccaaaa
     4321 gggtcaacct gtcttggttg gtacagtagc ggttgaaact agtgactaca tttctaagaa
     4381 attggttgca gctggtgttc ctcacgaagt cttgaatgcc aaaaaccact atagagaagc
     4441 ccaaatcatc atgaatgctg gtcaacgtgg tgccgttacc atcgcaacca acatggcggg
     4501 tcgtggtacc gacatcaagc ttggtgaagg tgttcgtgaa cttggaggac tttgtgttat
     4561 tggtacagaa cgtcatgaaa gtcgtcgtat cgataaccag cttcgtggac gttcaggtcg
     4621 tcaaggagat ccaggtgagt cacaattcta cctatctctt gaagatgatt tgatgaaacg
     4681 ttttggttct gaacgcttga agggaatctt tgaacgcttg aacatgtctg aagaggccat
     4741 tgagtctcgc atgttgacgc gtcaggttga agcagctcag aaacgtgtcg aaggaaataa
     4801 ctacgatacc cgtaaacaag tccttcaata cgatgatgtc atgcgtgaac aacgtgagat
     4861 tatctatgct caacgttacg atgtcatcac tgcagatcgt gacttggcac ctgaaattca
     4921 gtctatgatc aaacgcacga ttgaacgtgt cgttgatggt catgcgcgtg ccaaacaaga
     4981 tgaaaaacta gaggcaattt tgaactttgc taagtacaac ttgcttcctg aagattctat
     5041 tacgatggaa gacttgtcag gcttgtctga taaggccatc aaggaagagc ttttccaacg
     5101 ttccttgaag gtttacgata gtcaggtttc aaaactacgc gatgaagaag cagttaaaga
     5161 attccaaaaa gttttgattc tacgagtggt ggataacaag tggacagatc atatcgatgc
     5221 ccttgatcaa ttgcgtaacg cggttggact tcgtggctat gctcagaaca accctgttgt
     5281 tgagtatcag gcagaaggtt tccgtatgtt taatgatatg attggttcga ttgagtttga
     5341 tgtgacacgc ttgatgatga aagcacaaat tcatgaacaa gaaagaccac aggcagaacg
     5401 tcatatcagt acaacagcga ctcgcaatat cgctgctcac caagcaagta tgccagaaga
     5461 tttggatttg agccagattg gacgcaatga actttgccca tgtggttctg gtaagaaatt
     5521 taaaaactgt cacggtaaaa gacaataaaa tgagatagtt tagaggcgga tatcttgtga
     5581 aaagtaaatt tttactgggt atccgtttgc tttataagga gatgagttat ggtatttaca
     5641 gcaaaaagct ctaaaataaa tatagaagaa gttcgtgcct tgtcaaaatt agaaggtcag
     5701 gctttggaga ggaaatcaca gagagatcaa gagctagaag ccattatacg tggagaagac
     5761 cagcgaattc tcttggtaat cgggccatgc tcatctgata acgaagaagc tgtccttgaa
     5821 tacgctaagc gtttggcagt cctacaagaa gaagtggcag atcgtatctt tatggttatg
     5881 cgtgtttata ctgccaaacc ccgtaccaac ggagatggct ataaaggctt gattcaccag
     5941 cctaacgcga cagaagcgcc tagtcttatc aatggaatca aagccgttcg ccatcttcac
     6001 tatcgtgtca tcacagaaac agggatgaca actgctgatg aaatgcttta tcctgaaaac
     6061 cttccgcttg tagatgattt gatttcttac atggcagttg gtgcccgttc agttgaagac
     6121 cagcaacacc gctttgtggc aagtggggca ggattttcta ctggttttaa aaatccaacc
     6181 tctggaaatc tcaatgtcat gtttaatggg atttatgctg ctcaaaacaa acaaagtttc
     6241 cttttcttag gaaaagaagt agaaacaact gggaacccgc tttcacacgc tattcttcgt
     6301 ggtgctctta atgagtatgg aaaaaatatt cccaactact attatgacaa tttaattgat
     6361 accattgccc agtatgagaa aatgggcttg gaaaatcctt ttatcatcat tgataccaat
     6421 catgacaatt ctggtaagca gtatattgaa cagatccgaa ttgtccgcca gaccttgatt
     6481 aaccgtgctt ggaatgaaaa aattaagcag ttcgttcgtg gttttatgat tgagtcttat
     6541 ctagaagatg gtcgacaaaa tgagccagaa gtatttggta agtctatcac agacccttgc
     6601 ctgggttggg ataacacaga agctcttgtc agagaaattt acaaaacgtt aggagaataa
     6661 gatggcattt attgaaaaag gtcaagaaat cgatatggaa gtcatcaagg ctgaaaccca
     6721 attgtctgcg gaagccttga gactcaagga aagccgtgac agggaattgg cagatattat
     6781 ttcaggggaa gatgaccgta ttctcttggt gattggtcct tgctcttctg ataatgaaga
     6841 ggcggtcttg gaatatgctc gccgtttatc cgccttgcaa aagaaggtag cggacaagat
     6901 tttcatggtc atgcgcgtgt atactgctaa gcctcgtacc aatggagacg gctataaagg
     6961 attggttcac cagccagata cttctaaggc tccaagcctg attaacggct tgcaggctgt
     7021 gcgccagttg cactaccgcg tgattacaga gactggtttg acaacggcag atgagatgct
     7081 ttatccgtca aatctgatct tggtggatga cttggtcagc taccatgccg ttggagctcg
     7141 ttctgtggaa gaccaagagc accgctttgt ggcttctggg attgatgcac cagtagggat
     7201 gaaaaatcca acctcaggaa atttgggtgt tatgtttaac gccatctatg ctgctcaaaa
     7261 caagcaaacc ttcctttatc atgggcagga agttgagaca tcaggtaatc ctttggccca
     7321 tgttatcctc cgtggagcag tcaacgagta tggcaattat atgccgaatt actactatga
     7381 aaacctactc caagccattg aacgctatga aaccatggga cttgaaaatc cttttatcct
     7441 cattgacacc aaccatgata actcaggcaa gcaatatatg gagcagattc gaattgttcg
     7501 ccagaccttg cagaatcgtg attggaatga gaaaattaaa aagacggttc gaggatttat
     7561 gattgaatct tacctagcag atggtcgtca aaaccaacca gagatctttg gttgctctat
     7621 tactgaccct tgcctaggtt gggaaaatac agaggccttg gtagaagaga tttatgttac
     7681 cttgacaaaa taagtgaaaa ggatggagtt ggggaatctc aactcctttt gatgagaatg
     7741 atagttggac acggaattga catcgaagaa ttggcttcga tagaaagcgc agttacacga
     7801 catgaaggat ttgctaagcg tgtactgacc gctcaggaaa tggagcgctt caccagtctc
     7861 aaaggacgca ggcaaataga atatttagct ggtcgctggt cggctaagga ggccttttcc
     7921 aaggctatgg gaacgggcat tagcaagctc ggttttcagg atttggaagt cttgaacaat
     7981 gaacgtgggg cgccttattt tagtcaggca ccattttcag gaaagatttg gctgtctatc
     8041 agccacaccg atcagtttgt gacagccagt gtcattttgg aggaaaatca tgaaagctag
     8101 tccacataga ccaaccaagg ctctgattca tctgggagct attcgacaaa atattcagca
     8161 aatgggggct catatccctc aaggaacgct caagttggct gtggtcaagg ccaatgctta
     8221 tggtcatgga gctgttgccg ttgccaaggc aattcaagat gatgttgatg gcttttgcgt
     8281 ttccaatatc gatgaagcca ttgaactcag acaagctgga ctcagcaagc caatcctcat
     8341 tttaggagtt tctgaaatcg aagctgttgc tctagctaaa gaatatgact tcaccttgac
     8401 agtggctgga ctggagtgga ttcaagcact cttagataag gaagtggacc taactggatt
     8461 gacagtccac ctcaagattg attcagggat gggacggatt ggttttatag aggcaagtga
     8521 ggttgagcag gctcaagatt tactccaaca acacggtgtt cgtgttgaag gaatctttac
     8581 ccactttgct actgctgatg aggaatcaga tgactatttt aatgcccagt tagaacggtt
     8641 taaaactatt ttagctagta tgaaggaagt tccagagctg gttcatgcta gcaattctgc
     8701 aacgactctt tggcatgtag agactatttt caatgcggtt cgtatgggag atgccatgta
     8761 tggtctcaat ccaagtggag cggtcttgga tttgccttat gatttgatac cggccttgac
     8821 cttggagtct gctctggttc atgtcaagac agttccagct ggagcttgca tgggctatgg
     8881 agcaacttat caagcggata gcgagcaagt catcgcgacc gtgccaatcg ggtatgcaga
     8941 tggatggaca agagacatgc aaaatttctc tgtcttggta gatggccaag cttgcccaat
     9001 tgttggcagg gtttcgatgg accaaatcac tattcgattg cctaagcttt atccgctagg
     9061 aaccaaggta accttgattg gctccaatgg ggataaggaa atcactgcaa ctcaggtagc
     9121 gacctaccgc gtaaccatta actatgaggt ggtttgcctc ctcagcgacc gtattccgag
     9181 agaatattat tagaaaagaa aggagtggag catgaatcta catcaaccct tgcatgtctt
     9241 gcctggtgtg ggaccaaagt cagcagaaaa atacgccaaa ctaggaattg aaaacttgca
     9301 agatctcttg ctctactttc ctttccgtta tgaagacttc aaaaccaagc aggtgctgga
     9361 gctggaagac ggtgagaagg cagttctttc tggtcaggta gtgactcctg ctagtgtcca
     9421 gtattatggt ttcaagcgca atcgcctgcg ttttagtctc aagcagggag aggtcgtttt
     9481 tgcggtgaat ttctttaacc agccctatct ggctgataaa atagagttgg gagcaaccct
     9541 tgctgtcttt ggaaaatggg accgcgctaa ggctagtctg actgggatga aggttctggc
     9601 tcaggtagaa gatgacctcc agcctgttta tcgtctggct cagggaatca gtcaggccag
     9661 tctggttaag gtcatcaaga cggcttttga tcagggactg gacctcttaa tagaagaaaa
     9721 tctgccccag tctttactag acaaatacaa actcatgtcc cgttgtcagg cagtccgagc
     9781 tatgcatttt ccaaaggatt tggcagaata caagcaggct cttcgccgta taaagtttga
     9841 ggaactcttt tatttccaaa tgcagctgca gacgctcaag tctgaaaata gaattcaggg
     9901 aagtggtctg gttctggatt ggtctcagga aaaagtgaca gcagttaaag caagtcttcc
     9961 ttttgccctg acccaagctc aggaaaagag tttgcaggaa attttaactg atatgaagtc
    10021 cgaccaccac atgaatcgtc tcctacaagg ggatgtgggg agtggaaaaa cggtagtcgc
    10081 tggcttggcc atgtttgcgg cagtgacagc aggttatcag gctgccctaa tggtaccaac
    10141 agaaatcctc gcagagcaac actttgagag tttacagaac ctttttccca atttgaaact
    10201 ggctctcttg acaggttcct tgaaagctgc agaaaagaga gaagtcttgg agaccattgc
    10261 caagggtgag gctgatttga ttataggaac tcacgctctg atacaagatg gggtggagta
    10321 tgctcgtctt ggtttgatta ttatcgatga gcagcaccgt tttggtgtag ggcaaaggcg
    10381 tattttacgg gaaaaaggtg acaatccaga tgtcctcatg atgacggcga ctcccattcc
    10441 acggacgctt gccatcacag cctttggaga tatggatgtt tccattatcg accagatgcc
    10501 agcaggtcgg aagcctattg tgacgcgctg gatcaaacat gagcaactac ctcaggtctt
    10561 gacttggtta gagggggaaa ttcaaaaagg ttcccaagcc tatgtcatct ctcctttgat
    10621 tgaagaatca gaagctctag atttgaaaaa tgccattgcc ttatcagaag agctgacggc
    10681 tcattttgca ggcaaggcag aggtggctct tctacatggt aggatgaaga gtgacgaaaa
    10741 agaccagatc atgcaggatt tcaaggagag aaagacggat attctggttt cgacgacggt
    10801 tattgaggtt ggggtcaacg ttcccaatgc gactgtcatg attatcatgg atgccgatcg
    10861 cttcgggctc agccagcttc accagcttag aggtcgtgtc ggtcgggggg acaagcagtc
    10921 ctacgctgtt ctcgttgcca atcccaagac ggattctggg aaagaccgca tgcgcatcat
    10981 gacagaaacg accaatggat ttgtccttgc ggaggaagat ttgaaaatgc gtggttctgg
    11041 tgagattttt ggaaccagac agtcaggact tccagagttc caagtggctg atattatcga
    11101 agattttccg attttagaag aagcaagaaa ggttgctagc tacattagtt ctatagaagc
    11161 ttggcaagaa gatccagagt ggcgcatgct tgcccttcat ctggaaaaga aagaacatct
    11221 ggattaagct ttctctaagg aaaacttaag ctagttttta ggtttggctc ttatactaaa
    11281 gtcatcaaaa agaaacgagg actctcatat gacagtaacg attaaagtaa attaccaaac
    11341 cactttccaa aagaaggaag caaaaaacta gtataaacag aagagagagc gaaatgctct
    11401 ttttcgtttc taaaactact ttcagcccat catcctaaaa gtaaagaatc taaattcact
    11461 ttctatttac ccttctttct tgcattgatt acatagatat gctacagttg tggtaacgat
    11521 tacaaaataa aaggagcatg ctatgaaaaa tccagctttg ctagaagaaa ttaagaccta
    11581 tagaggaagg gatgaggttc cggaagactt tgatgctttc tgggatgggg aagtgaaaaa
    11641 tgtttccacg cttccatcct accacttgga ggaaagagat ttccacattc ctcaagtcaa
    11701 gtgctatgag ttaacatttg aaggaagcaa ggaaggaaag gtctatgcac gcattgttct
    11761 tccaaagagt gaggagaagg ttccattaat cttccatttt catggttata tgggacgtgg
    11821 ctgggactgg gccgacatgc tgggcttcac cgtagctggt tacggtgttg tttccatgga
    11881 tgtgcggggc cagtcaggtt actcacaaga cggcttgcgt tctcctttag gaaataccgt
    11941 gaaggggcat attatccgtg gtgctgtgga aggtcgggac cacctctttt ataaggatgt
    12001 ttatctggat atttaccagt tggtcgaaat tgttgctagt ctgtctcagg ttgatgagaa
    12061 gcgtctttct agctatggtg cctcacaagg aggggctcta gctctagttg cagcagcgct
    12121 caatcctcga attcagaaaa cagttgccat ttatcccttc ttgtcagact tcagacgggt
    12181 gattgagatt ggtaatacta gcgaggctta cgacgaactt ttccgttatt tcaagtttca
    12241 cgaccccttc catgaaacag aggaggaaat catggcgacc cttgcctata tcgatgtcaa
    12301 aaatcttgcc catcgtatcc aaggtgaggt taagatgatt acgggcttgg acgacgatgt
    12361 ttgctatccc attacccagt ttgcgattta taatcgtctg acctgcgata aaacctatcg
    12421 tatcatgcct gagtatgctc acgaagccat gaatgtattt gttaatgacc acgtctacaa
    12481 ctggctctgt ggaagtgaga ttccttttaa atatctaaaa taaggagtcg actctaagca
    12541 taaaatctta aaaattacaa acacgcatag tatcagggga ttaagaaaac ttgatactat
    12601 gcgttttatc atggaaatat agtaaaatga aataagaaca ggacaaatcg atca
//
LOCUS       DAVMGZ010000048        12613 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000048 DAVMGZ010000000
VERSION     DAVMGZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.049"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(84..248)
                     /locus_tag="VYQ93_001099"
     CDS             complement(84..248)
                     /locus_tag="VYQ93_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335798.1"
                     /translation="MATISLTRDIKLTNEDALKILNHEPSKKLQAVLKSIKSQDATAP
                     TKNKLILKYL"
     gene            complement(304..567)
                     /locus_tag="VYQ93_001100"
     CDS             complement(304..567)
                     /locus_tag="VYQ93_001100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335799.1"
                     /translation="MTYRICKPPSQSVYSGVCHRLAITSKIAIGFCSGVRHRLALMSK
                     ITIRLTMSRPLIYTIILTNMSYKRKRKTYFGFIHYRKHTLSLD"
     gene            709..1119
                     /locus_tag="VYQ93_001101"
     CDS             709..1119
                     /locus_tag="VYQ93_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein OmpA"
                     /protein_id="HEV3335800.1"
                     /translation="MEAIYQRDSDQDGLTDAQELALGTNPLSADSDGDGRSDLVEIEE
                     GTNPLEKDLQDIDQTSITEPSSVLMEMKQKISDMMESHYKEFIQALISIETGIENQQD
                     LEDLYTYYMRTDAVSLLSSDLEISPQEVEMEIEL"
     gene            1167..7385
                     /locus_tag="VYQ93_001102"
     CDS             1167..7385
                     /locus_tag="VYQ93_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV3335801.1"
                     /translation="MEVIQLLAMFRGTIPKDREKMDLFLRYQAQHFDEKWQDLVESFL
                     TKEGKIEEIPHVYSFHQDIVSFLEASSENNDQDLESYTRKFGQAGLSKLSQLSNFEKN
                     LVLEVATYNLSTRFYIQSEKEKLTPLSELVFHQNQDVNLVNVYRVANNLSDRISRDIE
                     EFLLMVDSKELKKELLEIHFEEKEGDVLAYLGSELMATLDTVTDLVHHEENYTQLPLT
                     QKLKIITHFDEVKAIREKSNQVEESLSPSSDIEQETEETNPFSNVDKIVEEALREYPI
                     GSQVSYKGQVFQLVSIENAQLNGLVRLELFNDSNQLFEENPILYLNSLEEIERVLSHV
                     ELEKEDSEIEMDSSSESQEIDLFSYLEEEKEKFNETESTPLISSLEELDIPVQDFVFP
                     DDLENFYPKTNREKIETNIAAIELVKRLEKEGRQANPEEQELLAKYVGWGGLANEFFD
                     ELNPKYEAERLTLKSLVSKSEYSAMKQSSLTAYYTDPMIIRQIWQKLLDDGFEGGRIL
                     DPSMGTGNFYAAMPRRIREKSELYGVELDSVTGAIAKQLHPNTHIEVRGFEEVSYQNN
                     SFDLVLTNVPFGNFRIADKNYDKPYMIHDYFVKHSLDLVRDGGQVSIISSIGTMDKRT
                     DNVLQEIKSNTHFLGGVRLPDTAFKSIAGTRVTTDLLFFQKDQAKNLNEEELVFSGSI
                     PFEEDKRVWINPYFDGKYNTQVLGQYEVRNFNGGTLNVKGESETLATDIMKALENVEA
                     PKQIDNSLKAPVFIQEEVDNSIPSRIREDLTLYSFGYEGNQIYYRDTHGIRKSSKVDE
                     ISYYVDEKGDFKAWDSSLSEHKIDRFVQLHLTDEEALDVYKSEEASKRGKYKGLFKKT
                     IFYESPLSDKDISRIKGMVDLRETYQSLIEIQRHPDYSRTDFQVLLSKLNRDYNRFVS
                     QFGYLNASVNRNLFDSDDKYSLLASLEDEYIDSKDQKVKYKKSLAFEKALVRPERVIT
                     RVSTALDALNSSLSDGRGVDLDYMVSIYPEHSQAAILDELGDQILMDPESYLRGERKY
                     LSKNQFLSGDILNKIEVVQLLVEENNQEYDWNHALDLLESVRPPRIHLADIEFKIGSR
                     WIPQSVYGKFAFECFTNREFELSSPDVEQVIEVNPVDGQVHLRTSFAYRYPSAKDSSL
                     GVSGSRYDTGRKIFENLLNSNQPTITMTVTEGEKKKTITDLEKTSVLRAKEQHLQELF
                     QEFVSQYPEVQQVIEESYNRLYNRTVSREYDGSHLVIDGLAQNISLRPHQENAIQRII
                     EEKRALLAHEVGSGKTLTMLGAGFKLKELGMVHKPLYVVPSSLSAQFGQEIMKFFPTK
                     KVFVTTKKDFVKARRKQFVSRIITGDYDAIVIGDSQFEKIPVSKERQMNYIEDKLNEL
                     REIKTHSENKYTVKEAEQSISGLEKQLEELQRFNRDSFIDFENLGIDFLFVDEAHHFK
                     NIRPITGLGNVAGITNTTSKKNVDMEMKVRQIQEEHDYKNIVFATGTPVSNSISELYT
                     MMNYIQPDILKRYQVDYFDSWVGAFGEIQNSMELAPTGDKYQPKKRFKKFVNLPELMK
                     IYKETADIQTQDMLDLPVPEAHIIPIESELTENQKLYLEELVMRSDMVKCGTVDPSQD
                     NMLKITGEARKLAIDMRLLDSSYSLADNHKLLQVVDNVERIYREGMENKATQMIFSDI
                     GTPKKKDNGFDVYSEIKALLVDRGVPSKEIAFVHDANSDEKKNSLSRKVNAGEVRILL
                     ASTEKGGTGLNVQSKMKAVHHLDVPWRPSDIQQRNGRIIRQGNENKEVDIYHYITKGS
                     FDNYLWATQENKLRYIKQIMTSKEPIRAAEDIDEQTMTASDFKALATGNPYLKYKMEL
                     ENDLTLLENQRRAFQRSKDHYRHTISYCEENIPILEKRLSKYEGDIQQSEMSKDQSFS
                     MTVGKQAFEQRAEAGESLHRLIRHNQADIKELRTLASYRGFDIKMLSLPTNQPLPETF
                     SVKIVGENRYSVSLDLYSPLGTIQRLQHTIDHIKDDQVKTQNLLDELKDKWTTAKIEI
                     EKNFPKEEDYQTKKAEYDVLAPLIETETDLDIIDQALRQFHEKGKEKQEQLSFELD"
     gene            7460..7744
                     /locus_tag="VYQ93_001103"
     CDS             7460..7744
                     /locus_tag="VYQ93_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335802.1"
                     /translation="MMEDTYYQLEEALVQGFQTPEEYQAYKELKEHYEEVTGDYSFSI
                     RELTSQLEIALQNHRGVDFEEHEKEEYLDLVQKLEEFDSSLATHYRQLID"
     gene            7758..8054
                     /locus_tag="VYQ93_001104"
     CDS             7758..8054
                     /locus_tag="VYQ93_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335803.1"
                     /translation="MNDLLLIPVIFLAVGGILILLWRLFLIASGLFLIGFISFLIFVE
                     VYGIYLFFTEPTLYFDDIRQHGLTSFTAVYLFINLMLVLGFSWRLINSKTKESM"
     gene            complement(8068..8265)
                     /locus_tag="VYQ93_001105"
     CDS             complement(8068..8265)
                     /locus_tag="VYQ93_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335804.1"
                     /translation="MKNLKEENLRRALSHIERHRQAINTSNNSEDNDFHKLLLQFSYE
                     VYERIKANKKPYPNLDSDKVF"
     gene            8425..9066
                     /locus_tag="VYQ93_001106"
     CDS             8425..9066
                     /locus_tag="VYQ93_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV3335805.1"
                     /translation="MLNKVKTKALISVGAVAATSFILMMGYTAGQHSTAKQSRKEIEL
                     AAAKLVENKQAEDKASILSSDTVKEFLTQYYTKEKLGENNTRIQPYMTESAYSQELSS
                     QNDAMNQVYKDYILDYHFEKADIFVNQTTNQAIAMVSYNVTYVSDLKNANQSKTNQTE
                     TRTVKLSYSKLPGKLLVNQVQVWKSGLDDLDKATPKTLEESSSIPSLPNTTTK"
     gene            9076..10161
                     /locus_tag="VYQ93_001107"
     CDS             9076..10161
                     /locus_tag="VYQ93_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HEV3335806.1"
                     /translation="MEIEECRQISILDVASRLGISFKQVSTSVYEHSEHDSFRIFSTT
                     NTFKWFSRDIQGDVIDFVRLVKGISFKEALAFLSEEPFQKEAVQEKRERPFYYPLKRV
                     EDSNCSLARYYLTECRGISEEIIQKMIQQGLIAQASWKTNETVEPVIVFKSYDHRHKL
                     QAASLQGIYKNHSLPRERLKTILKGSHGHIGISFDIGKPNRLVFCESFVDLISYYELH
                     QQSLTDVRLVSMEGLKRSVVAYQTLRLIAEENQKLEFLDTVTPSKLLHLINTIRDTTT
                     YFDNHPDLLTLAVDCDDAGKDFSDKLSQSGFPVLLDLPNNESGKEKIDWNDILREKKS
                     DLQLMIENVKETLRNQPVRQTSQCLEL"
     gene            10212..10445
                     /locus_tag="VYQ93_001108"
     CDS             10212..10445
                     /locus_tag="VYQ93_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335807.1"
                     /translation="MTIIERLEEKVTRQESKVARETEKLAAYREQLETAMFATFKRRQ
                     SISHMSFEEALDHAFGKERQFDDSEFRKDEMSE"
     gene            10442..10825
                     /locus_tag="VYQ93_001109"
     CDS             10442..10825
                     /locus_tag="VYQ93_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEV3335808.1"
                     /translation="MTKDWNFNQPLESKSENQEDPDKIAALFGNHQGGNDVNYEAAFQ
                     KRKQAPVTESNSSSKPKVTEVRTGKETDITTSYQQHLKRLIADNNSDIQSSQKKIEEL
                     HTLIDTKNKDNKKLQSIYDAISELH"
     gene            10863..11135
                     /locus_tag="VYQ93_001110"
     CDS             10863..11135
                     /locus_tag="VYQ93_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335809.1"
                     /translation="MFTKLFKKNQDNSDIFKKLIHRLSDMSVQDLEKIDRLLDIIFTP
                     DQGSEQLKTESTYREETLDDTLKEAKNQLHKEQLEKNLERFRKNSQ"
     gene            11206..11682
                     /locus_tag="VYQ93_001111"
     CDS             11206..11682
                     /locus_tag="VYQ93_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3335810.1"
                     /translation="MIGKNIKSLRKTHDLTQDDFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            11682..12443
                     /locus_tag="VYQ93_001112"
     CDS             11682..12443
                     /locus_tag="VYQ93_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV3335811.1"
                     /translation="MGIQDYTDSEFKQALERNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFARKMVESLV
                     TELSHLGYNLLIEGTLRTIDVPKKTAQLLKSRGYEVQLALIATKPKLSYLSTLIRYEE
                     LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT         4403 a         1944 c         2542 g         3724 t
ORIGIN      
        1 gaacttgttt ctcagtttta tcaggagcca gcacttgaat attttcaaga tattgcttaa
       61 gtggtgttac ttgaattgtc atcttacaga tactttaaaa tgagtttatt tttcgttgga
      121 gcggtagcgt cttgtgattt aatagatttc aagactgctt gtagcttttt agatggttca
      181 tgattaagaa tttttagagc atcttcgttt gttagtttta tatcacgtgt taaactgatt
      241 gtagccattt gttctacctc ctgtttctac ttaaattcat tatagcttat tttgaacaaa
      301 agttcaatct aaagatagtg tatgttttct atagtgtata aatccaaaat aagtttttct
      361 cttgcgtttg tagctcatat tcgttagaat aatggtgtag atgagtggtc ggctcatggt
      421 caatctgata gtaatcttgg acataagggc caagcggtgg cggacaccag aacaaaatcc
      481 gatagcaatc ttggacgtaa tggccaagcg gtggcagaca ccagaataaa ccgactgact
      541 aggtggctta cagattctgt aagtcatctt tttactttga taataatgtt ttatcatttt
      601 aaggagaacg cagttttggc tcgttctctt ttttgttgtg atacttcttt caaggaatat
      661 ttggtaggat tggagtgaaa aaagattaac taggaaagaa ggaagtatat ggaagccatt
      721 tatcaacgtg attcggatca agatggatta actgatgctc aagaattggc gctaggaacc
      781 aatcctctta gcgcagattc tgatggtgat ggacgttcag atttagtgga aatagaagaa
      841 ggaaccaatc ccttagaaaa ggatttacaa gacatagacc aaacaagtat cactgaaccg
      901 tcctcagtat taatggaaat gaaacaaaag atttcagata tgatggagag tcactacaag
      961 gaatttatac aggctctgat tagtattgaa acagggattg aaaaccaaca agacctagaa
     1021 gacttatata cttactacat gagaacggat gccgtttctc ttttatctag tgatttagaa
     1081 atcagtcctc aagaggttga aatggagata gagttgtagg ggaatcatgt gattctccta
     1141 ttttcgtata gatgaaagga gcgagtatgg aagtaataca attattggct atgtttcgtg
     1201 gaacaattcc aaaagataga gagaaaatgg acttatttct tcgttatcaa gcgcaacatt
     1261 ttgatgagaa atggcaggat ttggtagaga gttttttgac taaagagggc aaaatagaag
     1321 agatacctca tgtctattcg tttcatcaag atattgtttc tttcctagag gccagttctg
     1381 aaaataatga ccaagattta gaaagttaca caagaaaatt tggacaagca ggtctaagta
     1441 aattatctca attaagtaat tttgagaaaa acttggtgct agaagtcgca acctataacc
     1501 tttccactcg attttatatc caatctgaaa aagagaagct aacaccatta agtgagcttg
     1561 tatttcatca gaatcaggat gtcaatttag tcaatgtcta tcgggttgcg aataatctat
     1621 ctgaccgtat tagtagagat atagaggaat ttcttctaat ggttgattcc aaagaactaa
     1681 aaaaagaact tcttgagatt cattttgaag aaaaagaggg agatgttcta gcctatttgg
     1741 gttcagaatt gatggctact ttagataccg ttacggatct tgtccatcat gaagaaaact
     1801 acacacaact cccactgaca caaaagctga agattattac tcattttgat gaagtgaaag
     1861 ctataagaga aaagtctaat caagtagagg aatctttatc tccttcaagt gatattgaac
     1921 aggaaacgga agaaacgaac cctttttcta atgtcgataa aattgtagaa gaagctttga
     1981 gggaatatcc aattggttca caagtaagtt ataaaggaca agtatttcag ttggtttcga
     2041 ttgaaaatgc tcagttaaat ggcttagttc gcctagagct attcaatgat tccaaccagt
     2101 tatttgaaga gaatcctatc ttatacttga acagtttaga agagattgaa cgagtattgt
     2161 ctcatgtaga acttgaaaaa gaagattcag agattgagat ggattcatca agtgaaagtc
     2221 aggaaataga tttgttttcc tatctggaag aagaaaagga aaagtttaat gaaacagaat
     2281 caacaccact tatttcaagt ttagaagagt tggatattcc tgttcaagat tttgtttttc
     2341 cagatgattt agagaacttt tatcctaaga caaatcgaga aaaaattgaa acgaatatcg
     2401 ccgcaattga acttgttaaa agattagaaa aagagggacg acaagcgaat ccagaagaac
     2461 aagagctact agccaagtat gtcggctggg gtggtcttgc caatgaattt ttcgatgaac
     2521 tcaatccaaa gtatgaagca gaacgtttaa ctcttaagag tttagtaagt aaatcagaat
     2581 actccgccat gaaacaaagt tctctcacag cctattatac agacccaatg attattcgcc
     2641 agatttggca aaaattactg gatgatggtt ttgagggagg gaggatatta gatccttcta
     2701 tggggactgg gaacttctat gcggctatgc ctagaaggat acgagagaaa tcagaactct
     2761 atggggttga attagacagc gtgacaggcg caatcgcaaa acaactccac cccaataccc
     2821 atattgaagt gcgaggattt gaagaagttt cctatcaaaa taatagtttt gatttagtct
     2881 taacgaatgt cccatttgga aattttcgca ttgcagataa aaactatgat aaaccttata
     2941 tgattcatga ttactttgtc aaacactcac ttgatttagt aagagatgga ggacaagttt
     3001 cgattatctc ttctattggc acaatggata aacggacaga taatgtctta caagagatta
     3061 aatccaacac tcatttttta gggggagttc ggttgccaga tacggctttt aaaagcattg
     3121 caggtactcg tgtaaccaca gatctcctct tctttcaaaa ggatcaagca aagaatctta
     3181 atgaagagga acttgttttt agtggttcta ttccctttga ggaggataag cgtgtctgga
     3241 tcaatcctta ttttgatggg aaatacaata cacaagtttt gggtcaatat gaggtacgta
     3301 attttaatgg aggaaccctc aatgttaagg gggaatcaga aacattagct actgacataa
     3361 tgaaagcatt agagaatgta gaagctccta aacaaattga caattctttg aaagcacctg
     3421 tttttatcca agaagaagtg gataattcta tcccaagtcg tatacgtgag gacttaacgc
     3481 tctattcttt tggatatgag ggaaatcaaa tttattaccg agatacgcat ggcattcgga
     3541 aaagttcaaa agttgacgaa attagttatt atgtagatga gaagggagat tttaaagctt
     3601 gggacagttc tttgtctgaa cataaaatag accgattcgt gcaacttcat ttgactgatg
     3661 aggaagcact agatgtttac aagtcagaag aagcgagtaa aagggggaaa tataagggac
     3721 tgttcaaaaa aactatcttt tatgaaagtc ccttatcgga taaggatatt agtcgcatta
     3781 agggcatggt tgatttgaga gagacctatc aatccttaat tgaaattcaa cgtcatccag
     3841 attatagtcg gacagatttt caggtattac ttagtaaact caatcgtgac tataatcgct
     3901 ttgtaagtca atttggatac ttgaatgcct cagtcaatcg gaacttattt gatagtgacg
     3961 ataagtattc tttattagca agtttagaag atgaatacat tgattctaaa gatcagaaag
     4021 taaaatataa aaaatcttta gcctttgaga aagcattggt taggccggag agagtgatta
     4081 caagagtatc aacggcttta gatgccttaa actctagttt atcggatggt agaggggttg
     4141 atttagacta tatggtatca atttatcccg aacatagcca agctgctatt ttagatgagt
     4201 taggtgacca gattttaatg gatccagaaa gctatttaag aggggaaaga aaataccttt
     4261 ctaagaacca gtttttatca ggagatattc tcaacaagat agaagtagtt caactattag
     4321 tggaggaaaa caaccaagaa tatgattgga accatgcttt agatttgtta gaatctgttc
     4381 gccctccaag gattcatctg gcagatattg agtttaaaat agggtcacgt tggattcctc
     4441 aatccgttta tggtaaattt gcctttgaat gttttaccaa tcgtgaattt gaattgtctt
     4501 cgccagatgt tgaacaagtc attgaagtga atcctgtcga tggccaggtt catttaagga
     4561 catcatttgc ttatcgctat ccaagtgcca aagatagtag tcttggagtt agtgggtcac
     4621 gttatgatac aggaagaaag atttttgaga atttacttaa ttcgaaccaa ccgacgatta
     4681 ccatgactgt tacggaagga gaaaagaaaa agaccatcac agatttggaa aaaacctctg
     4741 ttctaagagc aaaagagcag catttacaag agctctttca agaatttgtc tcacagtatc
     4801 cagaagtcca acaagtcatt gaagaaagtt acaatcgtct ttataatcgg acggttagtc
     4861 gagagtatga tggtagccat ctagtcattg atggcttggc acaaaacatc agtcttcgtc
     4921 ctcaccaaga gaatgccatt caaagaatta tagaagaaaa aagagcctta ttagctcatg
     4981 aggtaggttc aggaaagacc ttgaccatgc ttggtgctgg gtttaaatta aaggagttgg
     5041 ggatggttca taagcccttg tatgtggtgc cctctagttt gtctgctcag tttggccaag
     5101 aaatcatgaa atttttccct actaaaaaag tctttgtgac cactaagaaa gattttgtga
     5161 aggcgagaag aaaacaattt gtttctcgta ttattacagg agattacgat gccattgtca
     5221 ttggggattc tcaatttgaa aaaatccctg tcagtaagga aagacagatg aactatatag
     5281 aggataaact caatgaacta cgagagatta aaacacattc tgaaaataag tataccgtta
     5341 aagaagcaga gcaatcaata agtggtctag agaaacaatt ggaagaactc caacgcttta
     5401 atcgtgatag ttttattgat tttgagaact taggaattga ttttctcttt gtggatgaag
     5461 cacatcactt taaaaatatt cgtccaatta ctggacttgg aaatgtagca gggattacca
     5521 atacaacctc taagaagaac gtggatatgg aaatgaaggt tcgacagatt caggaagaac
     5581 atgattataa aaatattgtc tttgcgacag gaacacctgt ttccaattct attagtgagt
     5641 tatatactat gatgaactat attcaaccag atattttaaa acgctatcaa gttgattatt
     5701 ttgactcttg ggtaggtgct tttggagaaa ttcaaaactc tatggaatta gctcctacag
     5761 gggataagta ccaacctaag aaacgattca aaaagtttgt caatctacct gagttgatga
     5821 aaatctataa agaaacagcc gacattcaaa cacaagatat gttggattta cctgttccag
     5881 aagctcatat tatccctatt gagagtgagt taactgaaaa ccagaaactc tatttagaag
     5941 aattggttat gaggtcagat atggtcaaat gtggaacagt tgatccgagc caggataaca
     6001 tgttaaagat aacaggtgag gcacgaaaac tagcaattga tatgcgttta ttggactcta
     6061 gttatagtct agcagacaat cataaattgc ttcaggtagt ggataatgtt gaaagaattt
     6121 atcgtgaggg aatggaaaat aaggctactc agatgatttt ttcagatatt ggaacaccta
     6181 agaagaagga caatggcttt gatgtttatt ctgagattaa ggctttatta gttgatagag
     6241 gagtccctag taaggaaatt gcctttgtac atgatgccaa tagtgatgaa aagaagaata
     6301 gcttgtctcg aaaggtcaat gcaggagagg tgcggattct tcttgcctca actgaaaaag
     6361 gaggaacagg tttaaatgtt cagagcaaga tgaaagcagt tcatcatctg gatgtaccgt
     6421 ggagaccaag tgacattcag caacgcaatg gacggattat ccgacaagga aatgaaaaca
     6481 aggaagtgga tatttaccac tatattacca aaggttcgtt tgataattat ctatgggcaa
     6541 ctcaggagaa caaactccgt tatattaagc agattatgac ttctaaggag ccgattcgtg
     6601 ctgcagaaga cattgatgag cagaccatga cagcttctga ttttaaggca ctagcaacag
     6661 gtaatcctta tctcaaatat aagatggaac tagagaatga tctaacccta ttagaaaatc
     6721 aaagacgcgc ctttcaacgt agcaaggatc actatcgtca tacaatctct tactgtgaag
     6781 aaaatattcc cattcttgag aaacgattaa gcaagtatga aggcgacatt caacagtctg
     6841 aaatgtcgaa agaccaatca ttttctatga cggtaggcaa gcaagctttt gagcaacgag
     6901 ctgaagcagg ggaatcccta caccgtctta tccgtcataa tcaagctgat attaaagaat
     6961 tacgtaccct agccagttat cgaggatttg acattaaaat gcttagtctt ccaacaaatc
     7021 aacctcttcc tgaaactttc tctgttaaga ttgtaggaga aaatcggtat tctgtcagtt
     7081 tagatttgta ttctcctttg gggacaattc aaagactcca acatacgata gaccacatta
     7141 aagatgacca agtgaaaact cagaacttat tggatgaatt aaaggataaa tggactactg
     7201 ctaagataga aattgagaaa aattttccaa aggaagagga ttatcaaact aaaaaggccg
     7261 aatacgatgt actcgcgcca ttgattgaaa cagaaacgga tttagatatt attgatcagg
     7321 ccttacgaca attccacgaa aaaggaaaag aaaagcaaga acaactttct tttgaattgg
     7381 attaaaaaat agatataaat agcggacaag aaaagaaaag cgcggtagaa taaagataga
     7441 aagaaacgag gtaacgatta tgatggaaga tacctattat caattagaag aggctttggt
     7501 acagggattt caaacacctg aagaatacca agcctacaag gagttaaagg aacattatga
     7561 ggaagtgaca ggcgattaca gcttttctat acgagaactt actagtcaat tggaaatcgc
     7621 tcttcagaat catcgaggtg tggactttga agaacatgag aaagaggagt atttggactt
     7681 agttcaaaaa ttagaagagt ttgattcctc tcttgccacc cattatcgtc aattgattga
     7741 ctagaaagga gaaagtcatg aatgatttac tccttatccc agtaattttt ttagcagtag
     7801 gaggaattct cattctttta tggagactct ttcttattgc cagtggactt ttccttattg
     7861 gttttatcag ttttcttatt ttcgtggagg tctatggaat ttatctgttc tttactgaac
     7921 cgactttata cttcgatgat atcagacaac atggtttaac tagttttacg gctgtgtacc
     7981 ttttcatcaa tctgatgttg gttctaggat tcagttggcg tttaataaac tccaaaacta
     8041 aggaaagtat gtaaacttgt tagatggtta aaaaacttta tctgaatcta aattcggata
     8101 aggtttttta ttagccttga ttctttcata tacttcatag ctaaattgga gcagtaattt
     8161 atgaaaatca ttatcctcac tgttattact tgtatttatg gcttgtctat gtcgttcaat
     8221 atgagaaaga gctcttctca aattttcttc ttttaaattt ttcatagtta cattataagc
     8281 tgaagacaga tagttttgat atttttctct gaactgtacg attttgcttc ttaaatgtag
     8341 gaatcacctt ttattatggt gtgataaaac ttacaagtcc tttttgttat tcttgaccta
     8401 gtttttatag aaagaaggtc taagatgtta aataaagtca aaactaaagc cttaattagt
     8461 gttggagcag tggctgcaac tagctttatt ctcatgatgg gatatactgc tggtcaacac
     8521 tcgactgcta aacaaagtcg taaagagatt gaattggctg cagctaaact tgtagagaat
     8581 aaacaagcag aagataaagc cagcattttg tcatcggata ctgtaaaaga atttttgacg
     8641 cagtactata cgaaagaaaa gctcggagaa aataatacac gtattcaacc ttatatgact
     8701 gaatcggctt attctcaaga attgtcaagt caaaatgatg ccatgaacca agtgtataag
     8761 gattatattt tagattatca ttttgaaaaa gctgatatct ttgtcaatca gactacgaat
     8821 caagccattg ccatggtctc atataatgta acctatgtat ccgatttaaa gaatgcaaac
     8881 caatcaaaga ccaatcagac agaaaccaga acggttaaat tgtcctattc taaactacct
     8941 ggtaagttat tggtcaatca agtacaggtt tggaaatctg gattagatga tttagataag
     9001 gccactccca aaactttaga agaatcatca tcaataccat cactgccaaa tactacgaca
     9061 aaatgatgat attgcatgga aatagaagaa tgtaggcaaa tttcaattct tgatgtagca
     9121 agtcgtttag gtatctcctt taaacaagtt tcgaccagtg tctatgagca ttctgaacac
     9181 gattcatttc ggattttttc aactaccaat acttttaaat ggttttcaag agatattcaa
     9241 ggtgatgtca ttgattttgt tcgacttgtt aagggaattt cctttaaaga agctctagcc
     9301 tttctttctg aagaaccttt tcaaaaagaa gctgttcaag aaaaaagaga gagaccattt
     9361 tattatcctt taaagagagt agaagattct aactgcagtc tggctagata ttatttaaca
     9421 gaatgtagag gaatctcaga agaaatcata caaaagatga ttcaacaagg tttgatagcc
     9481 caagctagtt ggaaaacaaa tgaaacagtt gaacctgtta tcgtttttaa aagctatgat
     9541 catcgtcaca agctgcaggc agcaagctta caagggattt ataagaatca ctctcttcct
     9601 agagagaggc tgaaaacgat tctaaaagga agccatggac atattggaat atcttttgat
     9661 attggtaaac caaatagact ggttttttgt gaatcgttcg tcgacttgat aagctattac
     9721 gaacttcatc aacaaagtct aactgatgtt cgtttggtat ctatggaagg attaaaaagg
     9781 tctgttgttg cttatcaaac tttacgacta atagctgaag aaaatcagaa gttggaattt
     9841 ttagatacag taacaccttc aaagttattg catttgatca atacaattcg tgataccacc
     9901 acctatttcg ataatcatcc tgatttattg acacttgcgg tagattgtga tgatgcagga
     9961 aaagattttt ctgataagtt atctcaatca ggatttcctg ttttactgga tttacctaat
    10021 aatgaatctg ggaaagaaaa aatagactgg aacgatattc ttagagaaaa gaaatcagat
    10081 ttacaattga tgatcgagaa tgtaaaagag acattgagga atcaaccagt aagacaaacc
    10141 tctcaatgtt tagaattgtg ataacaaaat caagatgttt tagctaatat tagaatgaag
    10201 gaggatcgta tatgaccata atcgagcgct tagaagaaaa ggtcactagg caagaaagca
    10261 aggtagcaag agagacagaa aaactagctg cttacagaga gcaactggag acagcgatgt
    10321 ttgcgacgtt caaaaggcgt caaagcatca gtcacatgag ttttgaagaa gctcttgacc
    10381 atgcctttgg taaagaaaga caattcgatg attctgaatt tagaaaggat gaaatgagtg
    10441 aatgacaaaa gattggaatt ttaatcaacc attagaaagt aaatcagaaa atcaagaaga
    10501 tccagataaa attgcggcct tatttggaaa tcatcaagga ggtaatgatg taaattatga
    10561 agcagctttt caaaagagaa aacaagcacc tgtgacagaa tcaaattcta gttctaaacc
    10621 caaagttaca gaggttagaa caggaaaaga aacagatatc actacaagtt atcagcaaca
    10681 tcttaaaaga ctgattgcgg ataacaatag cgatattcaa agcagtcaga agaaaattga
    10741 agagctacat acgttaattg acacaaagaa taaagataat aagaaattgc agtcaattta
    10801 tgatgcgatt tcagaattac attaagcagt cctaagaggc tgcttttttt gagaggctat
    10861 agatgtttac aaaacttttt aagaagaatc aagacaatag tgatatattt aagaagctaa
    10921 ttcatcgact atctgatatg tccgttcaag atcttgaaaa aatagaccga ctgctagata
    10981 tcattttcac tccagatcaa ggatcagaac aactaaaaac agaatcaact tatagagaag
    11041 aaactttgga cgacacatta aaggaagcaa agaatcaact tcataaggaa caattggaaa
    11101 agaatttaga gagatttagg aaaaatagcc aataatgcac caaaaatggt gcatttactt
    11161 tttctaaatg ttataatggt ggtataaaaa taaaaggagt ttgccatgat tggaaagaac
    11221 ataaaatcct tacgtaaaac acatgactta acacaagacg actttgcccg aattgtagga
    11281 atttctcgaa atagcttgag ccggtatgaa aatggaacga gttcagtctc tacggaacta
    11341 atagacatca tttgtcagaa gtttaatgta tcttatgtcg atattgtagg agaagataaa
    11401 atgcttaatc ctgttgaaga ttatgaattg actttaaaaa ttgaaattgt gaaagaaaga
    11461 ggtgctaatc tactatctcg actctatcgt tatcaagata gtcagggaat tagcattgat
    11521 gatgaatcta atccttggat tttaatgagt gatgatctat ctgacttgat tcatacgaat
    11581 atctatttag tagaaacttt tgatgaaata gagaggtata gcggctattt ggatggaatt
    11641 gaacgtatgt tagagatatc tgaaaaacgg atggtagcct aatgggaatc caagattata
    11701 ccgatagtga attcaaacag gctttagagc gaaaccttcg ctctctgaca agaggaaaaa
    11761 agtccagtaa gcaacctata gcgattttgc ttggagggca aagtggtgct ggaaagacta
    11821 caattcatcg tattaaacag aaagagtttc aaggaaatat tgttatcata gatggcgata
    11881 gttttcgttc tcagcatcca cactatttag aacttcagca agaatatggc aaagatagtg
    11941 ttgaatacac caaggatttt gcaaggaaaa tggtagaatc tttagtaaca gaattgagtc
    12001 atttgggata caatcttttg atagagggaa ctttacgaac gattgatgtt ccaaagaaaa
    12061 cagcacaact cttgaaaagt aggggatatg aagtacaatt agccttgatt gcgacaaagc
    12121 ctaagctgtc ctatctgagc acccttatcc gatacgaaga actatacgct atcaatccaa
    12181 atcaagcacg tgcaactcca aaagaacatc atgatttcat tgtaaatcat ctagttgata
    12241 atacacgaca attggaagaa ctggctatct ttgaaagcat tcaaatttac caacgagata
    12301 gaagttgtgt atatgattca aaagaaaata caacttcagc agcagatgtt cttcaagagt
    12361 tactctttgg ggagtggagt caggtagaga aggagatgtt gcaggtgggg gaaaagagac
    12421 ttaatgaatt acttgaaaaa taaacaattg atatttttag gagaatagaa atgagagggt
    12481 ttaataacaa gataaagtct gtttatcaag aactaacaaa ttccaaagag aaattcggta
    12541 gctttcacaa gactttaatt catttgcata cacctgtttc ttatgattac aagctatttt
    12601 ctaattggac tgc
//
LOCUS       DAVMGZ010000049        12340 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000049 DAVMGZ010000000
VERSION     DAVMGZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.050"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            16..382
                     /locus_tag="VYQ93_001113"
                     /pseudo
     CDS             16..382
                     /locus_tag="VYQ93_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
     gene            517..1476
                     /locus_tag="VYQ93_001114"
     CDS             517..1476
                     /locus_tag="VYQ93_001114"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV3335812.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            1573..2412
                     /locus_tag="VYQ93_001115"
     CDS             1573..2412
                     /locus_tag="VYQ93_001115"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV3335813.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLFKQLEANPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRIDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(2460..2630)
                     /locus_tag="VYQ93_001116"
     CDS             complement(2460..2630)
                     /locus_tag="VYQ93_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV3335814.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            2751..3635
                     /gene="miaA"
                     /locus_tag="VYQ93_001117"
     CDS             2751..3635
                     /gene="miaA"
                     /locus_tag="VYQ93_001117"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV3335815.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            3628..4866
                     /gene="hflX"
                     /locus_tag="VYQ93_001118"
     CDS             3628..4866
                     /gene="hflX"
                     /locus_tag="VYQ93_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV3335816.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            4859..5482
                     /locus_tag="VYQ93_001119"
     CDS             4859..5482
                     /locus_tag="VYQ93_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV3335817.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            5497..6426
                     /locus_tag="VYQ93_001120"
     CDS             5497..6426
                     /locus_tag="VYQ93_001120"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV3335818.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            6447..7202
                     /locus_tag="VYQ93_001121"
     CDS             6447..7202
                     /locus_tag="VYQ93_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV3335819.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(7256..8164)
                     /locus_tag="VYQ93_001122"
     CDS             complement(7256..8164)
                     /locus_tag="VYQ93_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV3335820.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            8228..8497
                     /locus_tag="VYQ93_001123"
     CDS             8228..8497
                     /locus_tag="VYQ93_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV3335821.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            8497..8877
                     /locus_tag="VYQ93_001124"
     CDS             8497..8877
                     /locus_tag="VYQ93_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV3335822.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            8966..9193
                     /locus_tag="VYQ93_001125"
                     /pseudo
     CDS             8966..9193
                     /locus_tag="VYQ93_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9233..9958)
                     /locus_tag="VYQ93_001126"
     CDS             complement(9233..9958)
                     /locus_tag="VYQ93_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV3335823.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            10120..11961
                     /gene="typA"
                     /locus_tag="VYQ93_001127"
     CDS             10120..11961
                     /gene="typA"
                     /locus_tag="VYQ93_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV3335824.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            11981..12235
                     /locus_tag="VYQ93_001128"
     CDS             11981..12235
                     /locus_tag="VYQ93_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV3335825.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLLLAYFSVRDITLMPVKKSKRR"
BASE COUNT         3596 a         2374 c         2806 g         3564 t
ORIGIN      
        1 aaatcaggcg gatacatggc taaaggtgaa tgggtttatg ttgccaccta tcaagcttgg
       61 tattatttga catcagatgg ttcttatgct tacagtacat ggcaaggaaa ttactatcta
      121 aaatcggatg gtaaaatggc tgtcaatgaa tgggttgatg gtggacgtta ttatgttggc
      181 gctgacggag tttggaagga aggtcaagca agtacagctt cttctagtaa tgatagcaat
      241 agtgaatatt ctgctgcttt aggaaaggca aaaagttata attcgttatt ccacatgtca
      301 aaaaaaacgt atgtatagac aattaacttc tgattttgat aaattttcaa atgatgcagc
      361 tcaatatgcc attgatcatt agatgattaa cagatactgg taggcgaaaa aattccttat
      421 aattcattta cgcaaacctt tgcataagtg aatggatttt gatatactag tactgtaagc
      481 attttcaatt actacacaat gaaaaaggag aatatgatga gtcaaaagat tattgggatt
      541 gaccttggtg gaacttctat caaatttgca atcttaacaa cagcaggaga aatccaagga
      601 aaatggtcaa tcaagaccaa cattttggat gagggaagtc atatcgttga tgatatgatt
      661 gagtctattc aacatcgttt ggacttgctt ggattggcag cagcggactt ccaaggcatt
      721 ggaatgggat caccaggtgt ggttgaccgt gacaaaggga ctgttatcgg tgcctacaac
      781 ttgaactgga aaacccttca accaatcaaa caaaagattg aaaaagcttt gggcattcca
      841 tttttcatcg ataatgatgc caacgtagca gctcttggtg agcgctggat gggtgctgga
      901 gataaccaac cagacgttgt ctttatgaca ctcggtactg gtgttggtgg tggtattgtc
      961 gcagaaggca aattgcttca tggtgttgct ggtgcagcag gtgaacttgg tcatatcact
     1021 gttgactttg accaaccaat ctcatgtact tgtggtaaaa aaggctgcct tgagacagtt
     1081 gcttcagcaa cagggattgt caacttgact cgtcgctatg ccgatgaata cgaaggcgat
     1141 gcagccttga aacgcctgat tgataacgga gaagaagtaa ctgctaagac tgtctttgat
     1201 ctcgcaaaag aaggagacga ccttgctttg attgtttacc gtaacttctc acgttacttg
     1261 ggaatcgctt gtgctaacat tggttcaatc ctaaacccat caacaatcgt catcggtggt
     1321 ggtgtgtcag ctgcgggaga attccttcta caaggtgttc aaaaagttta cgatgaaaat
     1381 agtttcccac aagtacgcac atctactaaa ttagctcttg caactctagg aaatgacgct
     1441 ggagttatcg gagcagcatc acttgtattg caataagata atgagagagg aaaatgctaa
     1501 cctagagtta gtaatgttcc tcttttattt tttatgctat actagttagg acaatgaatg
     1561 aggtgaaacg aaatgacaaa agcagatacg atttttaaag agaatattga acgcattcta
     1621 aaggaaggtg ttttctcaga gcaggctcgt cctaagtaca aggatgggac tgttgctaat
     1681 tccaagtatg taactggtgc cttttccgag tacgacttat ctaaaggaga atttcctatc
     1741 acaaccttgc gtcccattgc aatcaaatcc gccatcaaag aagttctctg gatttaccaa
     1801 gaccagtcaa atagcttaga ggtacttaat gataagtaca atgttcacta ctggaatgac
     1861 tgggaagtgg gggatacagg aactattggt gaacgctacg gtgctgttgt taagaaacac
     1921 gacatcatca acaagctttt caaacagttg gaagccaacc cttggaaccg ccgcaatatc
     1981 atttcgctct gggattacca agctttcgaa gaaacagatg ggctactccc atgtgccttt
     2041 cagaccatgt ttgatgtccg ccgtatagat ggagaaatct atctggatgc gaccttgacc
     2101 cagcgctcga atgacatgct agtagcccac cacatcaacg ctatgcagta tgtggctttg
     2161 cagatgatga ttgccaagca ttttggctgg aaggttggaa agttcttcta cttcatcaac
     2221 aacctccata tctatgacaa tcaatttgaa caagctcagg aattactccg tcgtgaaccg
     2281 tcaaactgcc aaccacgttt ggttttgaat gttcctgatg gaactaattt ctttgatatc
     2341 aaagcagaag attttgaatt ggtggattat gaccctgtta agccgcagtt gaagtttgac
     2401 ctagctattt aaaagaatag aaaaaagaag ttgagaataa tcccaacttc ttttgtttct
     2461 taacgtgata cgcggcgacg agctgctttt ttacggtttt cttcgatgaa agctgctttt
     2521 tgctcttctg gttcgattac tttcttttta aatgcgtata ctgcacctgc aacggcagcg
     2581 acagttcctg cgacacctgt tacaagacct ttagcgaatc ctttagccat gagtcttcct
     2641 cctttatatt ctcaatcagc cagcctcctc aagaggtcac atttttctga ctgacctttt
     2701 tgtgttataa taatagtaac gaaaaaatgg gaatttttca aggaaaaaag atgaaaacaa
     2761 aaataattgt gattgttgga ccgactgctg ttggaaagac ggctctagct attgaagttg
     2821 caaagcgttt taatggcgaa gtggttagtg gagatagcca acaagtctat cgaggacttg
     2881 atattgggac ggccaaggct agtccagaag agcaggcagc tgttcctcat catttaatcg
     2941 atgttagaga gataaccgag tcttactcgg cttttgattt tgtttcagaa gctaagatga
     3001 ctattgagga tattcagagc cgtggcaagc tagccattat cgccggtggg actggacttt
     3061 atatccagag cttgctagaa ggttaccacc taggtgggga gactcctcat gaggagattt
     3121 tagcttatcg agctagtttg gagccatatt cagatgagga attagcccat ttggtggagc
     3181 aagcaggcct tgagattccc cagtttaatc gtcgtcgtgc tatgcgtgcc ttagaaattg
     3241 cccattttgg tcaggatttg gaaaatcaag agatcttgta tgaaccgctg attatctgct
     3301 tggatgatga acgtagtcaa ctttatgagc gtatcaacca ccgagtggat ttgatgtttg
     3361 aggctgggct tttggatgag gccaagtggc tgtttgacca ttcccctaat gtacaggctg
     3421 ctaaaggaat tggctataag gaactctttc cttatttccg tggagagcag acctttgagg
     3481 aagctcgtga gagtcttaaa caggcgaccc gtcgttttgc caagcgtcag ctgacctggt
     3541 tccgtaatcg catgcaggtc accttttatc agattggaga atctggtgta caagaccgca
     3601 ttttaagcca gatagaggag tttttagatg attgaaacgg agaaaaaaga ggagcgagtc
     3661 ctgctgattg gtgtggaatt gcagggtatg gacagttttg acctctccat ggaagaattg
     3721 gctagtttag cgaaaacggc aggggcagtc gttgtagata gctacagaca aaaacgtgaa
     3781 aaatatgatt ccaagacctt cgtcggctct ggtaagttgg aagagattgc gcttatggtg
     3841 gatgcagaag aaatcactac tgtcatcgtc aacaatcgtc tgaccccaag gcagaatgtc
     3901 aatctagagg aagttctcgg tgttaaggtc attgaccgta tgcagttgat tttggatatc
     3961 tttgccatgc gggctcgaag ccatgaaggg aagctccaag tccacctagc ccaactcaaa
     4021 tacctcttgc ctcgcttggt tggtcagggg attatgctca gccgtcaggc agggggaatt
     4081 ggttcccgtg gtcctggtga aagccaactg gagctgaacc gtcgtagcgt tcgcaatcaa
     4141 atcacggata tcgagcgcca gcttaaggtg gttgagaaaa atcgtgcgac tgtcagagaa
     4201 aaacgtttgg agtctagcac ttttaagatt ggtttgattg gttataccaa tgctgggaaa
     4261 tcaactatca tgaacatctt gaccagtaag acccagtatg aagcagatga gctctttgcg
     4321 actctggatg cgacaaccaa gagtattcat ctgggaggca acctccaagt aactttgaca
     4381 gataccgttg gctttatcca agatttgccg acagagttgg tgtccagttt caagtcaacc
     4441 ttggaagaaa gcaagcatgt ggaccttctg gttcatgtta tcgatgctag caatccttac
     4501 cacgaggagc atgaaaaaac ggttctctcc atcatgaaag acctggacat ggaagatatt
     4561 cctcacttga cgctttataa taaagcggat ttggtggagg atttcacgcc tacccaaacg
     4621 ccatataccc tcatttctgc caagtctgag gacagtcgtg aaaacttgca agcattattg
     4681 ctagataaga ttaaggaaat ttttgaagca tttaccctgc gagtgccttt ttcaaagtcc
     4741 tacaagattc atgatttaga gagtgttgca attctggaag aacgtgatta tcaggaagac
     4801 ggcgaagtga ttacaggcta catttccgag aaaaataaat ggaggttaga agaattttat
     4861 gactgatatt aaaactttgg ctctaaagta tggaggttat acaagtctgg acaaggtcta
     4921 tctggatcag cttctagctg gtaaaacaga tcaggagcag ttaacactta ttacacctcc
     4981 gccaagtgtt gttaatgcct actttgcaga actctatcag aaaaagagtc ctcaggctgc
     5041 gacggattat tttgcagaac tcagtcagga actgaatctt tacaatactg agccaagttt
     5101 caccctagaa aataagcctt ttattcggct taatctgtct ggtaaatcct ttggtttttg
     5161 ttatgagagt gacggccttg gtcggatttt ctttgaaaat gaggagaaaa tctcggatga
     5221 cttgctcttt gaaattgcgc aaattttccc ccatcaatta gtctttgaag agtctggtaa
     5281 gatttacatg aagcctgtcg aggacgagga agttatcagc ctagaaagtc tcacagcttt
     5341 gactgatttg gaaagattgg ctgatggtcg aaaacgtctc aaaggctaca gccaagagga
     5401 tttattgcaa gaagctgctg ctttttctgg caaacgctat ttccgatcgg aaaaccgcac
     5461 agccatgtta tatattgatt aattagaaag tatcgaatgg atattcaatt tttaggaacg
     5521 ggggctggtc agccctctaa agcccgcaac gtttcaagtc tcgccctgaa acttttggat
     5581 gagattaacg aagtttggct ctttgactgt ggagaaggta cgcaaaatcg cattctggaa
     5641 accacaattc gaccacgtaa ggtcagcaaa atctttatta cccatctgca tggagaccac
     5701 atttttggtt tgccaggttt cctttctagc cgtgcctttc aggccaatga agagcagaca
     5761 gatttggaaa tctacggacc tcaaggaatc aagtcatttg tcttaaccag ccttcgtgtg
     5821 tcaggttctc gtctgcccta ccgcattcat ttccatgagt ttgaccaaga ttctctgggt
     5881 aaaattcttg aaaccgataa attcactgtg tatgcagagg agctggacca cactattttc
     5941 tgtgttggct atcgtgtcat gcaaaaggat ctagaaggga cgctggatgc tgaaaaactc
     6001 aaggctgctg gtgttccgtt cggcccgctt tttggtaaaa tcaaaaacgg ccaggatctt
     6061 gttttggaag acggaactga aatcaaggca gcagactata tctcagcgcc acgtccaggt
     6121 aagattatca ctattttagg agacactcga aaaacgggtg ccagtgtgcg tctggctgtc
     6181 aatgcagatg tcctagttca tgagtccact tatggcaagg gtgatgaaaa aattgctcgt
     6241 aaccatggtc actcaactaa tatgcaagct gcacaagtag cggtagaagc aggtgccaaa
     6301 cgcctcctac tcaaccatat cagtgcccgt ttcctctcaa aagatattag caaactcaag
     6361 aaagacgctg ccacaatttt tgaaaatgtc catgtggtca aagacttgga agaagtggaa
     6421 atctagcagt cacagaaagg ataagtatgc ctactattct cattaccgga gctagcggtg
     6481 gtctagctca agaaatggtc aaactcctgc ccaatgacca actcatcttg cttggtagaa
     6541 ataaggaaaa attagcccaa ctctacggaa attattccca tgcagaattg attgaaattg
     6601 atattaccga cgattcagcc ctagaagctc tggtaactga tctttatctc cgctatggca
     6661 agattgatgt cttgattaac aacgctggtt acgggatttt tgagggattt gaccagattg
     6721 ctgataaaga tattcaccag atgtttgagg tcaatacctt tgccctgatg aatctgtctc
     6781 gtcaccttgc ggctcgtatg aaggaaagca gcaaagggca tatcatcaac atcgtcagca
     6841 tggcaggtct aatagctact ggcaagtcta gtctttactc agcgaccaag tttgcggcta
     6901 ttggtttttc aaatgctctg cgactcgaac ttatgcccta tggagtctat gtgacaacag
     6961 tcaatccagg tccaatccga acaggatttt tcgaccaagc cgacccagat ggaacttatc
     7021 ttaaatcggt tgaccgcttc ctgctagaac cagatgcagt ggctaaaaag attgtcaaga
     7081 ttataggaaa aaacaaacga gaactcaatc tcccgatttt gttgaaccta gctcataagt
     7141 tttataccct ctttcctaag ctagctgata agttggcagg ggaaactttt aattataagt
     7201 aaaaagaacc aatgtgcagg ttgttgctag cctacatatt ggttcttatc atttctcaac
     7261 tatcaaactg aacttcttct aggagatagt ctataaagcg ttcgcccatc ttagataggc
     7321 tggttttctc atgctggata tagaccagct cgatcgggtc atcaatatcc agtgggatag
     7381 aaacgatatt gtctccgttt aggttgctgt tcaaaatccc tgtcgcaatg gtataaccat
     7441 ccaaaccaat caagagatta aagagggtgg cacggtcact gaccacaatg gatttcttgt
     7501 ggtgttcttg agaaagaatc tcttctgaaa agtagaagga gttgtgcgtc ccttggtcat
     7561 agctgaggta agggaaattc tccaaatcag acagtttcac cttgtctttc tttgccagag
     7621 ggttggtctt gctgacaaag atatgtggtt gcgctgtgaa gagatggtgg gctagcaggt
     7681 gattgtcatc cagcatcttg gttaaaacat cacggttgta actgtttaag aagaggaccc
     7741 cgacctcact gcggaagttc ttgacgtcgt cgataatctc ccaagtccga gtttcacgaa
     7801 ggaagagttc gtatttctcc atatcgcttt tcttgagcaa agagacaaag gcattgacca
     7861 caaaggcata gtgttgagac gaaacgctaa atagttcgcg gtgggcgaca ggatttttat
     7921 agcgttcctc cagaagctgg gtctgctcga caacctgacg ggcataagag agaaactcca
     7981 tcccatcacg ggtcaaggtg attcccttgg gattgcggat aaagatctca atgcccattt
     8041 cattttccaa atctcgcact gcattggaga gacttggctg agtgataaag agttgcttgg
     8101 ctgcctcatt catggagcca gtttcgacga ttttgataat ataatgtaat tgttgaattc
     8161 tcatgttttt attgtaccat acttgcctta gaatcgctaa agaagatagg aaaataagga
     8221 gcattgtgtg agtctcttct tgaaaaaaac acaaaatttt gctagaatga gtcttatgaa
     8281 aacattctat gatgtgcagc aattcctcaa acgatttggt attattgttt acatgggaaa
     8341 acgcttatat gatattgaac tgatgaagtt ggaactatct cggatttacg atgcagggtt
     8401 gatggataaa ttagactatc tagaggcaga agcagttctt cgcagagagc acaaggtaga
     8461 attggattat attgagaaaa atggagaaaa gaactaatgg ttacttggat tttgtgggca
     8521 cttatactag caatgttggc gtggatgggc tttaactatc ttcgtattcg ccgtgcggct
     8581 aaaattgtgg acaatgagga gtttgaagcc ttgattcgta cgggtcaatt gattgatttg
     8641 cgcgacccag cagaattcca cagaaaacat atccttggtg cacgcaatat tccttcaagt
     8701 cagttgaaaa ctagtcttgc agcccttcgt aaagataaac ctgtccttct ctacgagaac
     8761 caacgtgcgc aacgagttac aaatgcagct ctttatttga aaaaacaagg tttttctgag
     8821 atttatatcc tttcttatgg cttggattct tggaaaggga aagtgaagac tagctaacct
     8881 tatttccaaa ttagtcgaat gataggatga agctattgtt ttatagtata attaatttgt
     8941 ataaaattta aggagttctt agaacataaa agaaaagaag aaaaatccac tttttgtggg
     9001 tatcttgtcg attattcttg gtttgttatt tccaattgta ggcctgattt tgggtatcat
     9061 aggattggtc ttggctattt cataccaaaa agagtcataa ttagactata aaatagaaaa
     9121 gattcttaat atcttaggaa ttgtgatttc tgtagttaac tggattgtag caatcgcctt
     9181 aatttttcgg taaataaaag aaaaaagctt tagttttaaa gctttttttg ttttatcgac
     9241 tcatctctaa atagttttta atgatttgta actctttttg gttcaaaggg cggtagttac
     9301 tttctgctaa atctgggttc aatgtgaagt ccccaaattg gattctcttg aggatagtca
     9361 ccttaacacc aaccgagagg aacattttct tgatttgatg aaattttcct tctgaaatgg
     9421 tgatagaggc ttgactgatg gagggacttg cagatagaat ctctagtttt gcgggtttac
     9481 agacagtacc atctaaaaag acaattccct tttgaaaggc ttggatatgg tcaggtgtta
     9541 gaagtccatt aacctcaact tgataagtct tatcgacatg atattgggga tggaggagtt
     9601 ggaagcccaa gggaccgtta tcggtcaaga ggaggagtcc cgttgtatct cggtccagtc
     9661 ggccaacggc atagagcttg tcagactgga tgtcagatgg aagcaggtcc atgacggtcg
     9721 gaagttcctt gtctttgttg gctgtaacga caccagcagg tttatgaagc ataagatagg
     9781 tgtgttcata gccttgaatg attcgatcct gaaaaaggag ttcttgtagt cctgtatcga
     9841 tattttgagc tagggagcgg gctgggcaac catcgactag aattttccct ctgaggagtg
     9901 cttgcttcat ggcctttcgg ctgatttttt cttgggctaa taaattatct aaacgcatac
     9961 tgtgcctatc ttatcataag tcgcttgctt tgaaaaggct tgacgtgaaa agaaaagcgg
    10021 agtgaaaaca tttacacttg cttgagttat gttatttatt tgaaattatg gtataatcgt
    10081 tcagttagaa aataaatttt gaatattata gaggaaatca tgacaaaatt aagagaagat
    10141 atccgtaaca ttgcgattat cgcccacgtt gaccacggta aaacaaccct ggttgacgaa
    10201 ttattgaaac aatcagaaac gcttgatgca cgtactgaat tggcagagcg tgctatggac
    10261 tcaaacgata tcgaaaaaga gcgtggaatc accatccttg ctaaaaatac tgccgttgct
    10321 tacaacggaa ctcgtatcaa cattatggac acaccaggac acgcggactt cggtggagaa
    10381 gttgagcgta tcatgaaaat ggttgacggt gttgtcttgg tcgtagatgc ctatgaagga
    10441 accatgccac aaactcgttt cgtattgaaa aaagccttgg aacaagacct tgtcccaatc
    10501 gtggttgtta acaaaatcga taagccatca gctcgtccag cagaagtagt ggatgaagtc
    10561 ttggaacttt tcatcgagct tggtgcagat gacgaccagc ttgatttccc agtggtttat
    10621 gcttcagcga tcaacggaac ttcttcattg tcagatgatc cagctgacca agaagcgact
    10681 atggcaccaa tctttgacac gattatcgac catatcccag ctccagtaga taactcagat
    10741 gagcctttgc agttccaagt gtcacttttg gactacaatg acttcgttgg acgtatcggt
    10801 atcggtcgtg tcttccgtgg tacagttaag gttggggacc aagttaccct ttctaaactt
    10861 gacggtacaa ctaaaaactt ccgtgttaca aaactcttcg gtttctttgg tttggaacgt
    10921 cgtgaaatcc aagaagccaa agcgggtgac ttgattgccg tttcaggtat ggaagacatc
    10981 tttgtcggtg aaaccatcac tccgacagat gcagtagaag ctcttccaat cctacacatc
    11041 gatgagccaa ctcttcaaat gactttcttg gtcaacaact caccatttgc tggtaaagaa
    11101 ggtaaatggg taacttctcg taaggtggaa gaacgcttgc aggcagaatt gcaaacagac
    11161 gtttcccttc gtgttgaccc aactgattca ccagataaat ggactgtttc aggacgtgga
    11221 gaattgcact tgtcaatcct tatcgaaaca atgcgtcgtg agggctatga acttcaagta
    11281 tctcgtccag aagttatcgt aaaagaaatc gacggtgtta aatgtgaacc atttgaacgt
    11341 gtacaaatcg acactccaga agaataccaa gggtctgtta tccaaagcct ttctgaacgt
    11401 aaaggtgaaa tgttggatat gatttcaact ggtaatggtc aaactcgttt ggtcttcctt
    11461 gttccagcgc gtggtttgat tggatactca actgagttct tgtcaatgac tcgtggttac
    11521 ggtatcatga accatacctt cgaccaatac ttgccattga ttccagggga aattggtgga
    11581 cgtcaccgtg gtgcccttgt ttctatcgat gctggtaagg ctacaactta ctcaatcatg
    11641 tctatcgaag aacgtggtac gatctttgtc aacccaggta ctgaggttta tgaaggaatg
    11701 atcatcggtg aaaactctcg tgaaaatgac ttgacagtta acatcacgaa ggcaaaacaa
    11761 atgaccaacg ttcgttcagc tactaaggac caaacagctg ttatcaagac gcctcgtatc
    11821 ttgacacttg aagagtctct tgagttcttg aacgacgatg agtacatgga agtaacgcct
    11881 gagtctatcc gtttgcgtaa acaaatcctt aacaaagcag agcgtgagaa agctaacaag
    11941 aagaaaaaat cagctgaata agagctagaa agagagaaag atggtctatt tagtcctagg
    12001 aattttactg ctcctactct atgtatttgc gacaccagaa agcattaaag ggacagtcaa
    12061 tatcgtcgct atggtatgta ttttagtggc actcttgatt ttattggttc tatcttttct
    12121 gaaaattttt caattaccaa cagaaatatt cctagcaata gccatgttgc tcctagctta
    12181 ctttagtgtt agagacatta cactcatgcc agtcaaaaaa agtaaaagaa gataaaaaag
    12241 agagttttgg ctctcttttt tgctaggaat tttacaagat tgcaatttat ttttgagtta
    12301 tcaccatctc ttttgtttgg ataatatgaa tgggaaaaac
//
LOCUS       DAVMGZ010000050        12260 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000050 DAVMGZ010000000
VERSION     DAVMGZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12260
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.051"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..182
                     /locus_tag="VYQ93_001129"
     CDS             <1..182
                     /locus_tag="VYQ93_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335826.1"
                     /translation="LELIQGGADSYGKNPNGRYDWEIEPVLTLPVHGFCPRGTYDLGY
                     IGGGNHLCKGSAARF"
     gene            236..907
                     /locus_tag="VYQ93_001130"
     CDS             236..907
                     /locus_tag="VYQ93_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3335827.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(959..1156)
                     /locus_tag="VYQ93_001131"
                     /pseudo
     CDS             complement(959..1156)
                     /locus_tag="VYQ93_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1149..2057
                     /locus_tag="VYQ93_001132"
     CDS             1149..2057
                     /locus_tag="VYQ93_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV3335828.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2054..2515
                     /locus_tag="VYQ93_001133"
     CDS             2054..2515
                     /locus_tag="VYQ93_001133"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV3335829.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAILKEEINGN"
     gene            2505..3392
                     /locus_tag="VYQ93_001134"
     CDS             2505..3392
                     /locus_tag="VYQ93_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV3335830.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3395..5278
                     /gene="cbpE"
                     /locus_tag="VYQ93_001135"
                     /gene_synonym="pce"
     CDS             3395..5278
                     /gene="cbpE"
                     /locus_tag="VYQ93_001135"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV3335831.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            5358..6488
                     /locus_tag="VYQ93_001136"
     CDS             5358..6488
                     /locus_tag="VYQ93_001136"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV3335832.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6498..7760
                     /locus_tag="VYQ93_001137"
     CDS             6498..7760
                     /locus_tag="VYQ93_001137"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV3335833.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7764..8561
                     /gene="proC"
                     /locus_tag="VYQ93_001138"
     CDS             7764..8561
                     /gene="proC"
                     /locus_tag="VYQ93_001138"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV3335834.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            8781..9419
                     /locus_tag="VYQ93_001139"
     CDS             8781..9419
                     /locus_tag="VYQ93_001139"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV3335835.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILKEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            9416..10306
                     /locus_tag="VYQ93_001140"
     CDS             9416..10306
                     /locus_tag="VYQ93_001140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV3335836.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10346..10663
                     /gene="yabA"
                     /locus_tag="VYQ93_001141"
     CDS             10346..10663
                     /gene="yabA"
                     /locus_tag="VYQ93_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV3335837.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10666..11535
                     /gene="rsmI"
                     /locus_tag="VYQ93_001142"
     CDS             10666..11535
                     /gene="rsmI"
                     /locus_tag="VYQ93_001142"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV3335838.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETSLKGECLLIVEGASQDVEEKGEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            11762..>12260
                     /locus_tag="VYQ93_001143"
     CDS             11762..>12260
                     /locus_tag="VYQ93_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV3335839.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENIHSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRK"
BASE COUNT         3675 a         2104 c         2910 g         3571 t
ORIGIN      
        1 aattagagct gatacaaggt ggagcagatt catatggtaa aaatcctaat ggtaggtacg
       61 attgggaaat agaaccagta ttaactctgc cggttcatgg attttgtccc agaggcacct
      121 atgatttagg atatattgga ggaggcaatc atctttgcaa aggaagtgct gcgagatttt
      181 aagtaaaatt tattagtaat atgaagaaac aagtggagaa agcagagaat ttaatatgaa
      241 aaaacgggct attcaaattt tactagcatt gtccttaatt ttttacaaat caacttggtt
      301 ttggagagtt ttcaatcacc tcgcaaagcc ctatctacca gcaagtcgtg aattttttca
      361 gattctgctt ttgatggaga gcggagttct tttcttagcg gtcatctatc tactggtttt
      421 tgcaggaaag aaaatttttc atttcaagtg gcagctgagg tacttcatct accttttact
      481 gggctacatc atttcatata tgtctgactt cctcttttcg tatttcatat ccctgtcttc
      541 aaatcagatt tctttgaatg aaacggtaga aatgatgggg agacaggagt tcccttattt
      601 cttgctcatc gtttgcttca tcgcccctat tgctgaggaa ttgatttatc gaggtgtgct
      661 tatgacaacc tttttcaaaa actcaccttg gtacggagat gttttgcttt ctgctattat
      721 tttcggttat attcatatca attttgcttt aactcctctt gcttttttca tttatgctag
      781 tggaggtctt attttagctc tattgtatcg catgactaaa aatctctact atccaatact
      841 agttcatatt ctcattaata tcactgcctt ctgggatgtg tggttgctcc tattttcagg
      901 aagttagctt actaaaataa tgtcggaact ttccggcatt ttcttttttc acaaatagtc
      961 aacggttttc ttttcgatat tgtagtggtg tgtatccagt tatttttttg aattgatttt
     1021 gaaaataagg ttggcttgag aaaggcagat agtgaagata gttaagaaga agaggatgtt
     1081 cttttttcct ttttggaaaa cttctaaaat atggtataat gaaaagataa agaagttagg
     1141 ggtagaagat gaacattcaa caattacgct atgttgtggc cattgccaat agtggtactt
     1201 tccgtgaagc tgctgaaaag atgtatgtta gtcagccgag tctgtctatt tctgttcgtg
     1261 atttggaaaa agagttgggt tttaagattt tccgtcggac cagctcaggg actttcttga
     1321 cccgtcgtgg gatggaattt tatgaaaaag cgcaagaatt ggttaaagga tttgatattt
     1381 ttcaaaatca gtatgccaat cctgaagaag aaaaagatga attttctgtt gctagccagc
     1441 actatgactt cttgccacca actattacgg ccttttcaga gcgctatcct gactataaga
     1501 acttccgtat ttttgaatca actactgttc aaatattaga tgaagtggcg caagggcata
     1561 gtgagattgg gattatctac ctcaacaatc aaaataaaaa ggggattatg caacgggttg
     1621 aaaaattagg tctggaggtc atcgaattga ttcctttcca tacccatatt tatctccgtg
     1681 agggtcatcc tttagcccag aaagaggaat tagtcatgga ggatttagcg gatttaccaa
     1741 cggttcgttt cactcaagag aaagacgagt acctttatta ttcagagaac tttgtcgata
     1801 ccagcgctag ctcacagatg tttaatgtga cagaccgtgc caccttgaat ggtattttgg
     1861 agcggacgga cgcctatgcg acaggttctg gatttttaga tagtgacagt gttaatggca
     1921 ttacagttat tcgtctcaag gataacctag ataaccgcat ggtctatgtt aaacgtgaag
     1981 aagtggagct tagtcaagct gggactctct tcgtagaagt catgcaagaa tattttgatc
     2041 aaaagaggaa atcatgaaaa aaagagcaat agtggcagtc attgtactgc ttttaattgg
     2101 gctggatcag ttggtcaaat cctatatcgt ccagcagatt ccactgggtg aagtgcgctc
     2161 ctggattccc aatttcgtta gcttgaccta cctgcaaaat cgaggtgcag ccttttctat
     2221 cttacaagat cagcagctgt tattcgctgt cattactctg gttgtcgtga taggtgccat
     2281 ttggtattta cataaacaca tggaggactc attctggatg gtcttgggtt tgactctaat
     2341 aatcgcgggt ggtcttggaa actttattga cagggtcagt cagggctttg ttgtggatat
     2401 gttccacctt gactttatca actttgcaat tttcaatgtg gcagatagct atctgacggt
     2461 tggagtgatt attttattga ttgcaatact aaaagaggaa ataaatggaa attaaaattg
     2521 aaactggtgg tctgcgtttg gataaggctt tgtcagattt gtcagaatta tcacgtagtc
     2581 tcgcgaatga acaaattaaa tcaggccagg tcttggtcaa tggtcaagtc aagaaagcta
     2641 aatacacagt ccaagagggt gatgtcgtca cttaccatgt gccagaacca gaggtattag
     2701 agtatgtggc tgaggatctt ccgctagaaa tagtctacca agatgaggat gtggctgtcg
     2761 ttaacaaacc tcagggaatg gttgtgcacc cgagtgctgg tcataccagt ggaaccctag
     2821 taaatgccct catgtatcat attaaggact tgtcgggtat caatggggtt ctgcgtccag
     2881 ggattgttca ccgtattgat aaggatacgt caggtcttct catgattgct aaaaacgatg
     2941 atgcgcatct agtacttgcc caagaactca aagataaaaa gtctctccgc aaatattggg
     3001 cgattgttca tggaaatctg cctaatgatc gtggtgtaat tgaagcgccg attggccgga
     3061 gtgaaaaaga ccgtaagaaa caggctgtaa ctgctaaagg gaagcctgca gtgacgcgtt
     3121 ttcacgtctt ggaacgcttt ggcgattata gcttagtaga gttgcaactg gagacagggc
     3181 gcactcatca aatccgtgtc cacatggctt atatcggcca ttcagtcgct ggtgatgagg
     3241 tctatggtcc tcgcaagact ttgaaaggac atggacaatt tcttcatgcc aagactttag
     3301 gttttactca tccgagaaca ggtaagacct tggaatttaa agcagatatc ccagagattt
     3361 ttaaggaaac cttggagaga ttgagaaagt aagaatgaaa aagaaattaa ctagtttagc
     3421 acttgtaggc gcttttttag gtttgtcatg gtatgggaat gttcaggctc aagaaagttc
     3481 aggaaataaa atccacttta tcaatgttca agaaggtggc agtgatgcga ttattcttga
     3541 aagcaatgga cattttgcca tggtggatac aggagaagat tatgatttcc cagatggaag
     3601 tgattctcgc tatccatgga gagaaggaat tgaaacgtct tataagcatg ttctaacaga
     3661 ccgtgtcttt cgtcgtttga aggaattggg tgtccaaaaa cttgatttta ttttggtgac
     3721 ccatacccac agtgatcata ttggaaatgt tgatgaatta ctgtctacct atccagttga
     3781 ccgagtctat cttaagaaat atagtgatag tcgtattact aattctgaac gtctatggga
     3841 taatctgtat ggctatgata aggttttaca gactgctgca gaaaaaggtg tttcagttat
     3901 tcaaaatatc acacaagggg atgctcattt tcagtttggg gacatggata ttcagctcta
     3961 taattatgaa aatgaaactg attcatcggg tgaattaaag aaaatttggg atgacaattc
     4021 caattccttg attagcgtgg tgaaagtcaa tggcaagaaa atttaccttg ggggcgattt
     4081 agataatgtt catggagcag aagacaagta tggtcctctc attggaaaag ttgatttgat
     4141 gaagtttaat catcaccatg ataccaacaa atcaaatacc aaggatttca ttaaaaattt
     4201 gagtccgagt ttgattgttc aaacttcgga tagtctacct tggaaaaatg gtgttgatag
     4261 tgagtatgtt aattggctca aagaacgagg aattgagaga atcaatgcag ccagcaaaga
     4321 ctatgatgca acagtttttg atattcgaca agacggttta gtaaacattt caacttccta
     4381 caagccgatt ccaagttttc aagctggttg gcataagagt tcatatggaa actggtggta
     4441 tcaagcgcct gattctacag gagaatatgc tgtcggttgg aatgaaatta agggtgaatg
     4501 gtattacttt aaccaaacgg gtatcttatt acagaatcaa tggaaaaaat ggaacaatcg
     4561 ttggttctat ttgacagact ctggtgttgc tgctaaaaat tggaagaaaa tcgctggaat
     4621 ctggtattat tttaacaaag aaaaccagat ggaaattggt tggattcaag ataaagagca
     4681 gtggtattat ttggatgttg atggctctat gaagacaggt tggcttcaat ataaggggca
     4741 atggtattac tttgctccat caggggaaat gaaaacgggc tgggtaaaag ataaagaagc
     4801 ctggtactat atggattcta ctggtatcat gaagacaggt gagatagaag ttgctggaca
     4861 tcattactat ctagaagaat caggagctat gaagcaaggc tggcttaaaa aggcaaatga
     4921 ttggtatttc tacaagacag atggttcacg agctataggt tggattaaag acaaggataa
     4981 atggtacttc ttgaaagaaa atggtcaatt acttgtgaat ggtaagacac cagaaggcta
     5041 tactgtggat tcaagtggtg cctggttagt ggatgttccg atcgagaaat ctgctacaat
     5101 taaaactaca agtcattcag aaataaaaga atccaaagaa gtagtgaaaa aggatcttga
     5161 aaataaagaa acgagtcaac atgaaagtgt tacaaattct tcaactagtc aagatttgac
     5221 atcctcaact tcacaaagct ctgaaacgag tgtaaacaaa tcggaatcag aacagtagta
     5281 gaaaagaagg ttttagggcc ttctttttcc tatcaactct tttctatttc ctgttattca
     5341 tgttataatg gataaatatg aataatcgga gtgagactat gaaatacaaa cggattgtct
     5401 ttaaggtggg tacttcttct ctgacaaatg aggatggaag tttatcacgt agtaaggtaa
     5461 aggatattac ccagcagttg gctatgctgc acgaggctgg tcatgagttg attttggtgt
     5521 cttcaggtgc cattgcggct ggttttggag ccttaggatt taaaaagcgt ccgactaaaa
     5581 ttgctgataa acaggcttca gcagcggtag ggcagggact tttgttggaa gaatatacga
     5641 ccaatcttct cttgcgtcaa atcgtttctg cacaaatctt gctgacccaa gatgactttg
     5701 tggataagcg tcgttataaa aatgcccatc aggctttgtc ggttttgctc aaccgtgggg
     5761 caattcctat catcaatgag aatgatagtg tcgttattga tgagctcaag gttggggaca
     5821 atgacactct aagtgctcaa gtagcggcga tggtccaagc agacctttta gttttcttga
     5881 cagatgtgga cggtctctat actggaaatc ctaattcaga tccaagagcc aaacgcttgg
     5941 agagaatcga gaccatcaat cgtgagatta ttgatatggc tggtggagct ggttcgtcaa
     6001 acggaactgg gggtatgtta accaaaatca aggctgcaac tatcgcgacg gaatcaggag
     6061 ttcctgttta tatctgctca tccttgaaat cagattccat gattgaggcg gcagaggaga
     6121 ccgaggatgg ttcttacttt gttgctcaag agaaggggct tcgtacccag aaacaatggc
     6181 ttgccttcta tgctcagagt caaggttcta tttgggttga taaaggggct gcggaagctc
     6241 tctctcaaca tggaaagagt cttctcttat ctggtatcgt tgaagcagaa ggagtctttt
     6301 cttacggtga tatcgtgaca gtatttgata aggaaagtgg aaaatcactt ggaaaaggac
     6361 gcgtgcaatt tggagcatct gctttggagg atatgttgcg ttctcaaaaa gccaagggtg
     6421 tcttgattta ccgtgacgac tggatttcca ttactcctga aatccaacta ctttttacag
     6481 aattttagag gtaaactatg gtgagtagac aagaacaatt tgaacaggta caggctgtta
     6541 aaaaatcgat taacacagct agtgaagaag tgaaaaacca agccttgcta gccatggctg
     6601 atcacttagt ggctgctact gaggaaattt tagcggctaa tgccctcgat atggcagcgg
     6661 ctaaggggaa aatctcagat gtgatgttgg atcgtcttta tttggatgca gatcgtatag
     6721 aagcgatggc aagaggaatt cgtgaagtgg ttgccttacc agatccaatc ggtgaagttt
     6781 tagaaacaag tcagcttgaa aatggtttgg ttatcacaaa aaaacgtgta gctatgggtg
     6841 tcatcggtat tatctatgaa agccgtccaa atgtgacgtc tgatgcggct gctttgactc
     6901 ttaagagtgg aaatgcggtt gttcttcgta gtggtaagga tgcctatcaa acaacccatg
     6961 ccattgtcac agccttgaag aagggcttgg agacgactac tattcatcca aatgtgattc
     7021 aactggtgga ggatactagc cgtgaaagta gttatgctat gatgaaggcc aagggctatc
     7081 tagaccttct cattcctcgt ggaggagctg gcttgatcaa tgcagtggtt gagaatgcga
     7141 ttgtacctgt tatcgagaca gggactggga ttgtccatgt ctatgtggat aaggatgcag
     7201 acgaagacaa ggcgctgtct atcatcaaca atgctaaaac cagtcgtcct tctgtttgta
     7261 atgccatgga ggttctgctg gttcatgaaa acaaggcagc aagcatcctt cctcgcttgg
     7321 atcaaatgct ggttgcagat cgaaaagaag ctgggttgga accaattcaa ttccgcctag
     7381 atagcaaagc aagccagttt gtttcaggtc aagctgctca agcacaagac tttgataccg
     7441 agtttttaga ctatattcta gctgttaagg ttgtgagcag tttagaagaa gcggttgcgc
     7501 atattgaatc ccacagtacc catcattcgg atgctattgt gacggaaaat gctgaagctg
     7561 cagcatactt tacagatcaa gtggactctg cagcggtgta tgttaatgcc tcaactcgtt
     7621 tcacagatgg aggacaattt ggtcttggtt gtgaaatggg gatttctact cagaaattgc
     7681 acgcgcgtgg tccaatgggc ttgaaagagt tgaccagcta caagtatgtg gttgctggtg
     7741 atgggcagat aagggagtaa gagatgaaga ttggatttat cggtttgggg aatatgggtg
     7801 ctagcttggc aaaatctgtc ttgcagacta ggacgtcaga tgagattctc cttgccaatc
     7861 gtagtcaagc taaggtagat gctttcattg cagactttgg tggtcaggct tccagcaatg
     7921 aagaaatgtt tgcagaagca gatgtgattt ttctaggagt taagcctgct cagttttctg
     7981 aactgctttc tcaatatcag accatccttg aaaaaagaga aagtcttctt ttgatttcga
     8041 tggcagctgg attgacctta gaaaaactag caagtcttat cccaagccaa caccgaatta
     8101 ttcgtatgat gcctaatacc cctgcttcta tcgggcaagg agtgattagt tatgccttgt
     8161 ctcctaattg cagggctgag gacagtgaga tcttttgtca gcttttagcc aaggctggtc
     8221 tcttggttga attaggagaa agcttaatca atgcagcgac aggtcttgca ggttgtggac
     8281 cagcctttgt ctatctcttt attgaggcct tggcagatgc aggtgttcag acgggattac
     8341 cacgagaaac agccttgaaa atggcagccc aaactgtggt aggagctggg caattggtcc
     8401 tagaaagcca gcaacatcct ggagttttga aagatcaagt ttgtagtcca ggcggttcga
     8461 ctattgctgg cgtagcaagc ctagaagcgc atgcttttcg aggcacagtc atggaggcag
     8521 ttcatcaagc ctataaacga acacaagaac taggtaaata agaggtagtt ttatctgcct
     8581 cttttatggt ggttgatact cttcgaaaat caaattcaaa ctgcgtcagc ttcaccttgc
     8641 cgtactcaag tacagcctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat
     8701 gaaatgagaa gacaaaaaga ttgtcacaaa cccctatttt tttgatagaa tagaagtagt
     8761 aaaaaagaaa tgagttagac atgtcaaaag gatttttagt ctctcttgag ggaccagagg
     8821 gagcaggcaa gaccagtgtt ttagaggctc tgctaccaat tttaaaggaa aaaggagtag
     8881 aggtgttgac gacccgtgaa cctggcggag tcttgattgg ggagaagatt cgggaagtga
     8941 ttttggatcc aagtcatact cagatggatg ctaaaacaga gctacttctc tatattgcca
     9001 gtcgcagaca gcatttggtg gaaaaagttc ttccagccct tgaagctggc aagttggtca
     9061 tcatggatcg ttttatcgat agttctgttg cctatcaggg atttggtcgt ggcttagata
     9121 ttgaagccat tgactggctc aatcagtttg cgacagatgg cctcaaaccc gatttgacac
     9181 tctattttga catcgaggtg gaagaagggc tggctcgtat tgctgcgaat agtgaccgcg
     9241 aggttaatcg tttggatttg gaagggttgg acttgcataa aaaagttcgt caaggctacc
     9301 tttctcttct ggataaagag ggaaatcgca ttgtcaagat tgatgctagt ctccctttgg
     9361 agcaagttgt ggaaactacc aaggctgtct tgtttgacgg aatgggcttg gccaaatgaa
     9421 acaagatcaa ctaaaggctt ggcaaccagc tcagtttgac cgttttgtcc gtatcttaga
     9481 acaagaccag ctcaatcacg cctatctctt ttcaggtttc tttggaagct tggaaatggc
     9541 gcaattttta gctaagagcc tcttttgtac ggataaagtt ggcgtcttac catgtgagaa
     9601 atgccgaagt tgcaagctga ttgaacagga agagtttcca gatgtcacct tgattaagcc
     9661 agtcaatcag gtcatcaaga cagaacgcat tcgggaattg gtgggacagt tttctcaagc
     9721 agggattgaa agccagcaac aggtctttat tatcgagcaa gcggataaaa tgcatcccaa
     9781 cgcagccaat tctctgctca aggtcatcga agaaccccag agtgaagttt atattttctt
     9841 cttgactagc gatgaggaaa agatgttacc gacaatccga agtcggactc agatcttcca
     9901 ctttaaaaag caagaagaaa aacttatctt actcttagaa caaatgggac ttgttaagaa
     9961 aaaagcgact cttttagcta agtttagtca atcgcgagct gaagcagaaa agttggctaa
    10021 tcaggcaagt ttttggacct tggtcgatga aagtgaacgc ctgctgactt ggttagtagc
    10081 taagaaaaaa gaaagttatc tacaggttgc caaattagcc aacttggcag atgataagga
    10141 aaaacaggat caggttttac ggattcttga agttctctgt gggcaggacc tcttgcaggt
    10201 aagagtaaga gtgattctac aagatttact agaagctaga aaaatgtggc aagctaatgt
    10261 cagctttcaa aatgccatgg aatatctggt cttgaaagaa atataaactc aaaaatgaat
    10321 gataaagaaa ggaaagggct gttttatgga caaaaaagaa ttatttgacg cgctggatga
    10381 tttttcccaa caattattgg taaccttagc cgatgtggaa gccatcaaga aaaatctcaa
    10441 gagcttggta gaagaaaata cagctcttcg cttggaaaat agtaagttgc gagaacgctt
    10501 gggtgaggtg gaagcagacg ctcccgtcaa ggccaagcat gttcgcgaaa gtgtccgtcg
    10561 tatttaccgt gatggatttc acgtatgtaa tgatttttat ggacaacgtc gagagcagga
    10621 cgaagaatgt atgttctgtg atgagttgtt atacagggag taagcatgca aattcaaaaa
    10681 agttttaagg ggcagtctcc ctatggcaag ctgtatctag tggcaacgcc gattggcaat
    10741 ctagatgata tgacttttcg tgctatccag accttgaaag aagtggactg gattgctgct
    10801 gaggatacgc gcaatacagg gcttttgctc aagcattttg acatttccac caagcagatc
    10861 agttttcatg agcacaatgc caaggaaaaa attcctgatt tgattggttt cttgaaagca
    10921 gggcaaagta ttgctcaggt ctctgatgcc ggtttgccta gcatttcaga ccctggtcat
    10981 gatttggtta aggcagctat tgaggaagaa attgcagttg ttacagttcc aggtgcctct
    11041 gcaggaattt ccgccttgat tgccagtggt ttagcgccgc agccacatat cttttacggc
    11101 tttttaccga gaaaatcagg ccagcaaaag caatttttcg actcgaaaaa agactatcct
    11161 gaaactcaga ttttttatga atcgccccat cgtgtggcag atacattgga aaatatgcta
    11221 gaagtctacg gtgaccgctc cgttgtcttg gtcagggaat tgaccaaaat ctatgaagaa
    11281 taccaaagag gtacaatttc tgaattgctg gaaagcattg ctgaaacgtc tctcaagggt
    11341 gaatgtcttc tgattgttga aggtgccagt caggatgtgg aagaaaaagg cgaggaagac
    11401 ttgttcttag aaatccaagc tcgtattcag caaggtatga agaaaaatca agctattaag
    11461 gaagtagcta agatttacca gtggaataag agtcaactct acgctgccta ccacgactgg
    11521 gaagaaaaac aataaaggga gacaggatgt aataattctg tctgtttctg tttaacttaa
    11581 ttagtgatga taatataaag atgtatcact tggtatagaa gctttggtat taagtttttt
    11641 attaagccca tacggaatac cgatggttgg agcagcagtt atagcgttct tagaaggtat
    11701 aaatagaaaa ataaggtcat tttaaatcaa aggattgata aatcagaaag aaggtgattt
    11761 tttgcgaaca tacgaaaata aagaagaact aaaagctgag atagagaaaa catttgagaa
    11821 atatatttta gaatttgata atattccaga aaatttaaaa gataagagag ctgatgaagt
    11881 tgacagaact ccagcagaaa accttgctta tcaggttggt tggaccaact tggttcttaa
    11941 atgggaagaa gatgaaagaa aggggcttca agtaaaaaca ccatcggata aatttaaatg
    12001 gaatcaactt ggtgaattat atcagtggtt cacagatacc tacgctcatt tatctctgca
    12061 agagttgaaa gcaaaattaa atgaaaatat tcattctatc tctgcaatga ttgattcgtt
    12121 gagtgaggaa gaattatttg aaccgcatat gagaaagtgg gctgatgaag cgactaaaac
    12181 agcgacttgg gaagtgtata agtttattca tgtaaatacg gttgcacctt ttggaacttt
    12241 cagaactaaa atcagaaaat
//
LOCUS       DAVMGZ010000051        12252 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000051 DAVMGZ010000000
VERSION     DAVMGZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12252
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.052"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..1149)
                     /locus_tag="VYQ93_001144"
     CDS             complement(<1..1149)
                     /locus_tag="VYQ93_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3335840.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINQN"
     gene            1207..2373
                     /locus_tag="VYQ93_001145"
     CDS             1207..2373
                     /locus_tag="VYQ93_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV3335841.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            2512..2838
                     /locus_tag="VYQ93_001146"
     CDS             2512..2838
                     /locus_tag="VYQ93_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV3335842.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            2825..3418
                     /locus_tag="VYQ93_001147"
     CDS             2825..3418
                     /locus_tag="VYQ93_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV3335843.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            3411..4322
                     /locus_tag="VYQ93_001148"
     CDS             3411..4322
                     /locus_tag="VYQ93_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV3335844.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDFITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTDNHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            4384..5448
                     /locus_tag="VYQ93_001149"
     CDS             4384..5448
                     /locus_tag="VYQ93_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335845.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADFDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEVGEARTEIEEKKASNSTEEESDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKFETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(5626..6486)
                     /locus_tag="VYQ93_001150"
     CDS             complement(5626..6486)
                     /locus_tag="VYQ93_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV3335846.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIYLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILG
                     QDFKTLAQEK"
     gene            complement(6608..7594)
                     /locus_tag="VYQ93_001151"
     CDS             complement(6608..7594)
                     /locus_tag="VYQ93_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV3335847.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(7866..9341)
                     /locus_tag="VYQ93_001152"
     CDS             complement(7866..9341)
                     /locus_tag="VYQ93_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV3335848.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKATDSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(9599..10522)
                     /locus_tag="VYQ93_001153"
     CDS             complement(9599..10522)
                     /locus_tag="VYQ93_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3335849.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(10536..11465)
                     /locus_tag="VYQ93_001154"
     CDS             complement(10536..11465)
                     /locus_tag="VYQ93_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3335850.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(11784..12134)
                     /locus_tag="VYQ93_001155"
     CDS             complement(11784..12134)
                     /locus_tag="VYQ93_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335851.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRNILQTVKDAI
                     VSLESIKESQSATKDGGQTVGKETVDKDVLENSQEELQKAVLFLADEKGSEHTAAELI
                     DNLKEVIAKLKANA"
BASE COUNT         3648 a         2612 c         2250 g         3742 t
ORIGIN      
        1 attttgattt atttggctta ttttatccaa ataaattatt gttctcttgt actccttctt
       61 ccatttaaat cctctaatta aacctacaat cgtcccaagg ccataagcaa agtgaatgga
      121 aaataaaaga aagggcatca caattagaaa tccattttta tgttttaata aagtcagcaa
      181 agtgagtaat gacaaaagta gaaaataggc acctaatagt aaagttatga atacgaatgt
      241 gatcggtaac aatgctagac taaacacaag actcaaaaca aataaacaag gaacatagtg
      301 aaataatgat aaacacttaa actgaacatg acttgtcaag ccaatccaca aaccatttga
      361 atacttttga tgcagcattt tcttgaatgt tggtcgaata tactgataag atagaatact
      421 tgggctatag cggattttat aaccatattc tcgaattcta taatgaatat cattatcttc
      481 agttcggcca agttgctcat ttactaaacc aaccttctgg aaaacctctc gtttatacat
      541 tccatgaaaa atagaagaaa catatctatc ctcagaacta tttcgataat tggcaatgct
      601 actgccaaac atattttcct caacaagatg caaggtctct gcccattttc cttttccttc
      661 gacaatcgtc ggtctaggcc ccccacagac aaattcacct tgttgaataa tagccacatt
      721 gttcattaca aaagtctcag taacttttga atgagcatca atttttaaaa taaggtcccc
      781 tacagaatgt ttaactccca ggttaaaacc actagcttga tttttcttag gattgttata
      841 caatctaatt gagttaaact ctgtatcttc ctttataaat tgctgaatga tagctgtggt
      901 cccatctgtg gacatagcat ttataaatag aatttcaata ttctctttag gataggtttg
      961 attttttaag tcttcaatta gaccaggcag atatttttct tcattgtatg cactgattac
     1021 aatggatacg agaagacttt caacattttg agtcactact actctcctga atttttcctt
     1081 ccaccattct atcataattt tcaagacttt cccattttta tcttgaaagc cagaaacctt
     1141 acttgacatt atagtgaaaa agccttaaaa taagctgtta ttaaaatcag ctagaagagg
     1201 tattatatga aaaagcaatc actctttttt gttccaggta ttatcctgat tggtgtttcc
     1261 ttgcgaactc cttttactgt tttacccatt attttgggaa atatttcgca aggtctggag
     1321 gtagaagtta gttcgcttgg tgtcttgacc agcctgcctc tccttatgtt taccctcttt
     1381 tcaccatttt ctacccaact ggctcagaaa atcggcttgg agcatctctt cacctacagc
     1441 ctcttcttct tgaccatcgg ctcacttatt cgactaatca atctgcccct gctctatcta
     1501 ggaaccttga tggttggggc aagtgtcgca gtcatcaatg tcctgcttcc tagtcttatc
     1561 caagccaatc aaccaaagaa aattggtttt ctgaccacct tatatgtaac gtctatgggg
     1621 attgcaacgg ctctggcttc ctatctagct gtgcccatta cacaagccag ttcttggaaa
     1681 ggacttatcc ttctcctcac gttactctgt ctagcaactt ttttggtctg gctcccaaat
     1741 caccgctata atcatagact agctccacaa accaaacaaa aaagtcaaat aaaggtcatg
     1801 cgtaataaac aggtttgggc aattattatc ttttcaggtt ttcaatcctt gatcttttac
     1861 accgtcatga cctggttacc taccatgtct atccatgcag gtctatccag tcacgaagct
     1921 ggcttgctga cttctatctt atctctgatt agcattcctt tttcaatgac catcccaagc
     1981 ctgacaacca gtttatctac tcgcaaccgt cagctcatgc tcactctggt ttcactagct
     2041 ggtgtggtcg gcatttccat gctctttttc ccaatcaata atttcattta ctggcttacc
     2101 atccatctcc tcatcggaac cgcaaccagc gccctcttcc cttatctcat ggtcaacttt
     2161 tcactcaaga caagcgcccc tgaaaagaca gcccaattgt ccggcctatc tcaaacagga
     2221 ggctatatcc tagcagcctt tgggccaacc ctctttggtt acagttttga cctgttccac
     2281 tcttgggtac cagctgtagc tgccctcttg ctcatctata tcctgatgac tgtggccctc
     2341 tttacagtgg acagagcgga taagatcctt taaacttcta gttttagcta gaagtttttt
     2401 atatataaaa attttacaca tttctcttga cagggctttc ttttagatgt acaatgtatg
     2461 tgtagaaaaa ttatatataa aaatcttaca cattagaaaa ggaggtttcc catgtacttt
     2521 ccaacatcct ctgccttgat tgaatttctc atcttggctg tactggagca gggtgattct
     2581 tatggttatg agattagcca aaccattaag ctgatcgcta atatcaaaga atccacactc
     2641 tatcccattc tcaaaaaatt ggaaggcaat agctttctga caacctattc tagagagttc
     2701 caaggtcgca tgcgcaaata ctactccttg acaaacggtg gtatagagca gctcttgacc
     2761 ctaaaagatg aatgggcact ctatacagac accatcaatg gcatcataga agggagtatc
     2821 cgccatgaca agaactgaat acctgactca gctagaactc tatctcaaga aactacctga
     2881 agctgaccgt atcgaagcca tggactattt cagagagctc tttgacgatg ctggagtcga
     2941 aggagaagaa gaactcatcg ctagtttggg aactcccaaa gaagcggccc acgaagttct
     3001 atccaatctt ctcgataaaa aaatcaatga agcacccgct caaaaaaata accgacaaat
     3061 tttacatatc gccttgttag ccctccttgc agcacctatc ggcattcctc tgggaatcgc
     3121 catcctcgtg accctgttcg caatccttgt agccgctttg actgtcattc tggctttctt
     3181 tgcagtttcc atactgggta tcatcggcgg attcctattt ttagttgaaa gtttcactat
     3241 cctcgcccaa gccaaatcag cctttatctt gatttttggt tctggtttac tggctatcgg
     3301 tgcttcttcg ctagttttac ttggcatttc ctatgtagct cgcttcttcg gtctactcat
     3361 tgttcgtctg gtacaatttg ttcttaaaaa aggaaagaga ggtaatcagc atgcgtaaat
     3421 ggacaaaagg atttctcatc tttggtgtgg tgactaccgt tatcggcttt atcctgcttt
     3481 ttgtaggtat ccaatctgac gggattaaga gcctactttc catgtccaaa gaacctgtct
     3541 atgatagccg tacggaaaag ctaacctttg gcaaggaagt cgaaaaccta gaaattactc
     3601 tccaccaaca cacgctcacc atcacagact ctttcgatga tcaaatccac atttcttacc
     3661 atccatctct ttctgctcac catgatttta tcaccaatca gaacgataga actctgagtc
     3721 tcactgataa gaaactgtct gaaactccgt ttctctcttc tggaattggt gggattcttc
     3781 atatcgcaag tagctactct agtcgttttg aagaagttat tctccgacta ccaaaaggga
     3841 gaactctaaa agggatcaac atctcagcca atcgcggaca aaccaccatc ataaatgcta
     3901 gccttgaaaa tgcgaccctc aatacaaaca gctatatcct ccgaattgaa ggaagtcgta
     3961 tcaaaaacag taaactcaca acgcccaata tcgttaatat ctttgataca gttcttacag
     4021 atagtcagct agagtcaaca gataatcact tccacgctga aaatatccaa gtccatggta
     4081 aggttgaact gactgccaaa gattatctca gaatcatcct agaccagaaa gaaagccaac
     4141 gaattaactg ggacatctca agtaactacg gttctatctt ccaattcaca agagaaaagc
     4201 ctgaatcaag aggtacggaa ttaagcaacc cttacaaaac tgaaaaaacc gatgtcaagg
     4261 atcaactcat tgcgagatct gatgataata ttgatctaat atccacacca agcagacgtt
     4321 gacaaaaacg cttacatctg ctactatgga agcatattta tctaaccaaa aggaggttct
     4381 accatgaaac aagaatggtt tgaaagtaat gattttgtaa aaacaacaag caagaacaag
     4441 cctgaagagc aagctcaaga ggttgcagac aaggctgaag aaacgatagc cgatttcgat
     4501 acaccaattg gaaaaaatac tcaagtagag gaggaagtct ctcaagctga agtcgaactg
     4561 gaaagccagc aagaagagaa aattgaaact cctgaagtcg gtgaagcgag aacagaaata
     4621 gaagaaaaga aggcatctaa ttctactgaa gaagagtcag acctttctaa agaaacagaa
     4681 aaagtcacta tagctgaaga gagccaagaa gctcttcctc agcaaaaagc aaccacgaaa
     4741 gagccgcttc ttatcagtaa atctttagaa agtccttata tccccgacca agctccaaaa
     4801 tctagggata aatggaaaga gcaagtgctt gatttttggt cttggctagt ggaagcgatc
     4861 aaatctccta caagtaagtt tgaaacaagt atcacacaca gttacacagc ctttctcttg
     4921 ctcattctgt tttctgcatc ttcctttttc tttagtatct atcacatcaa acatgcttac
     4981 tatggacata tagcaagcat taacagtcgc ttccctgagc agctagctcc tttaactctt
     5041 ttttctatca tctctatcct agtagcgaca acactcttct tcttttcatt cctcttgggt
     5101 agtttcgttg tgagacgatt tatccaccag gaaaaggact ggacactaga caaggttctc
     5161 caacaatata gtcaactctt ggcaattcca atcttcctca ctgctattgc tagtttcttt
     5221 gctttctttg atagcctacg atttacagcc ctcttgtgtg tgattagcat tggaatcatt
     5281 ctgcttgcca gtctccatat cattacaaga ccaagtcaag caagtgaaac cgactccttc
     5341 tatcaattat tcttgtctgt ccttgtgaac ggagttatta tcctcctctt ctttgtagct
     5401 gaagtggcac tgattggaga ttatcttcgt atcttggcct ttctttaaac ttcatactct
     5461 tcgaaaatca aattcaaacc acgtcagctt cgccttaccg tactcaagta cagcctgcgg
     5521 ctagcttcct agtttgctct ttgattttca ttgagtagca atcctgtcat ccttggcagg
     5581 attttttcta tgtcaaaaaa gcaagtcgat tgacctgctt ctgctttact tttcttgtgc
     5641 taatgtttta aaatcttgtc ctaggatggg ttgtatttct atttgattgg cgtctagttg
     5701 gtggtaaaaa gatgggggta atacctctaa gaattttgca tagtccaagc gagctatgga
     5761 ttcatagtct tctggtttag agccgtctcc tgtgatttga accaagtaaa tctcccactg
     5821 gacttcctta tccaccaatt gctcaatata ggctgcagga acaaacggtt ctctcggtaa
     5881 aactgtcttg actccttgag ctaaatggta aacgccatgc acttttcctt ctccatcctg
     5941 atagaaggaa actcttgcaa agtaggcatc tgtctcttga accgcacgca cacgcgcttc
     6001 aaactgagcc aagccatctg cctctaaaaa gcttttcaag ttctcacgac taaagaaaat
     6061 aggattaaaa attccttggc aaatcgtaaa acgagctaca gggtctgcta gataggcttg
     6121 aatacttgct tggaaataag cttcaaaatc aaaaccatct agcccttcaa tatcagtccc
     6181 tgtataagca agcaaagcat ctaaaaatga cttgataatc tgccagtctg tctttgttag
     6241 taggtcagga agatcgacac ggtaagcctt ttgaccatca gcataactga ccttaaagag
     6301 aaattgtgat tgccccacta tcgcacactc aatatactcg agacgattaa gaggctgacg
     6361 aagatagact acatcatagc tatgtgactc caaaccgtct agcaaggcca agatcgactt
     6421 ggcattcaaa atctcttgtt ctcctaaaat gctctgttta tttggaataa aaaatgtttt
     6481 tgccataact ggactccttt gaaatcgttt acatacagta taccttattt tcctgaaaga
     6541 tacaaaaaca aactttagat ttttgagtac aaagcataga aaaacagccc ctgaggactg
     6601 tttgatctta tacagtagct gcctgatcca agctttcacc gatagcggct aggcgctcaa
     6661 tcacttcagc ttgtgtcaat tcattttttg aaacatagcg gttacgtggg tgaacacggc
     6721 actcgtgtga gcatccacga aggtacttgt cttcattttc ttctgatgtc aagatacgac
     6781 ggttacagaa gggatttcca cagttgacat aacgttcaca tggtgttcca tcaaaccagt
     6841 ctttccctac gatggttggg ttgacatggt tgacatcaac ggcaatacgc tcgtcaaaga
     6901 cgtacatttt cccatcccaa agctcacctt gaacttctgg gtctttaccg taagttgcga
     6961 ttcctccgtg caattggccg acatctttgt agccttcacg gaccatccag cctgagaatt
     7021 tctcacagcg aacgccacct gtacagtaaa ccacgacacg cttgtccatg aatttttcct
     7081 tgttatcacg gacccattgt ggtaactcac ggaagttgcg aatatctggg cgaatagccc
     7141 cacggaaatg tcctaggtcg tactcataat cgttacgtgt gtcaaggaca acggtatctt
     7201 tatcaagaag cgcttctttg aactcttttg gagacaagta agcacctgtt gtttcaagtg
     7261 ggttgatgtc attgtcaaag tcgttgtctt ccaaaccaag gtggacaatt tctttcttgt
     7321 agcgaacaaa catcttcttg aaggcttgtt cattttcttc gtcaatcttg aaccagagat
     7381 cttccatacc tgggaggctg tgaacgtagt ccatgtattt ttgagttgtt tcatagtcac
     7441 ctgaaactgt tccgttaatt ccctcgtcag cgactaggat acggccttta aggccgattg
     7501 atttacagaa agccaagtgg tctgcagcaa attgctctgc attttcaatt ggagtataaa
     7561 ggtagtaaag taagacacga atatcttttg ccataagatt tgttctcttt tctattctta
     7621 aattttctaa attttttatt tatccatact agtatacctg cttgagaaaa cgaatgcaat
     7681 ttatttgact ggaaattgta gaggattctc gaaatttcct gaatggttaa aataaggaca
     7741 agagaaaaca gggatatcta tatccttgtg ccaaaaaaac cactgccctc cccagatcaa
     7801 cctgaggaaa gcagtgattc ttattttagg agttaggaat gaatacacga aatcaattta
     7861 gctgattatt ttttgttttt caagaattca tcgtattgtt tttgcatttc gttcaatact
     7921 ttttcgtagg caccttcaga tttcaatttt tccatcaatt ctggaatcgc tttatctggg
     7981 tctacagtac cagtgttgat agctgtatca aattgttgca ttgtgttagc aatagctgag
     8041 atttcagatt tcacattgtc agtattaaag ataaatccaa gcgctggaga ttctttagct
     8101 tctgccaatt ctttcttaga attttcgatt tgttggtctg taacgttttc gttgatgtaa
     8161 aggatccagt tgttaccagt gttccatcca cccatgtgag tgtttccttt gtagccatca
     8221 agaacgcgaa cacggttttc tttaccttca attttttccc agttcttgcc ttctggaccg
     8281 taaacaagac cgttcaagag ttctgggttc gtattcaaga ggttcaagat ttccattgat
     8341 ttttctttgt tcttagagtt gtttgagatg acaaagttag caacttgtgt tgtttggttt
     8401 ttcttgatga agttagtaat tggtttgatt tggatatctt tgttggcaac acgtgaaagc
     8461 aagctgttac cgtagtcagc tggtcctact gtttcttcac gaacgaacca agtatcttgt
     8521 tgaaggtcaa aggaagtatc gcttgttgcg acgtcttttg gaatgtagcc agcttcatag
     8581 aatttgtgaa gagtcttcaa gtgttctttg aaacgaggta cttcgtaacg gtttacaact
     8641 ttagtagtat cgccttcaag gtcaataacg aatggaagac cgtttgctac tgggtagtca
     8701 aagttatcag atgggatgaa aactttacca atagcaaatg gtactacgtc tggagctttt
     8761 tctttgattt gtttcaagac tggctcaaga gtttcgtaag aagtaacacc tgaaatatcg
     8821 ataccatatt tggcaaggag agttccgttg aaggcaaagt tttgagatga tgctacgtta
     8881 gctgcaactg gtacagcata aatcttacca tttacagtat tacccttgat ataagctgga
     8941 tcaagtgcct tgtaaaggtc tttaccttct tttttgtaca attctgtcaa gtcagcataa
     9001 gcaccttttt gagcatttac aatatagtta tctgcaaagg cgatatcata gttttcacca
     9061 gatgatgtga taactgacat tttcttacca tagtcacccc agccaaggta ttggatatcc
     9121 aatttagcac caactttttc ttcgatgatt ttgttggcat ttgctaacaa ttcatccaag
     9181 ttatctggtt tgtcaccgat ttggtacatt ttgataacag gtttgtcacc tgaatcagta
     9241 gcttttttgc tgttacctgt caaatttcca caagcagcaa gacctgcagc caaagcgact
     9301 acactagcag atgcaaaagc atattttttc cagtttttca tgatgaaaac tccttttttt
     9361 atttttaaac ttataaacaa tgtaatgatc ttatactcaa tgaaaatcaa agagcaaact
     9421 agaaaactag ccgcaggttg ctcaaaacac tgttttgagg ttgtggataa gactgacgaa
     9481 gtcagttaca tatatctacg gcaaggcgac gttgacgcgg tttgaatttg attttcgaag
     9541 agtattaact tcacacaagg gaagttggga actgagaaat gttctttttc aataagcact
     9601 attctttcac accaccgata gtcaaacctt ttacaaagta gcgttggaag aatggatata
     9661 aaatcgcgat tggaagggtt gcaaccacaa ccatggccat acgacctgtt tctttcggta
     9721 gagcaactcc cagttgacca gtcaagccga ccgctttggc aatgtagtcc atattttgtt
     9781 ggatttgcat gagcaaatat tgcaatggat acaagttatc actcttgatg taaagaaggg
     9841 cgttgaacca gtcattccag aaaccaagag ctgtcaaaag cgtgatggtt gcgatacctg
     9901 gtagtgacaa tggcaaacag atttggaaga aaatccgggc ctcactggca ccatcgatac
     9961 gagccgattc gagaatggct tctggaatgg tcttcttgaa gaaggaacgc atcaagatga
    10021 tattgaatgg tgagagaagc attggaacaa tcaaggccca aacagtgtca ccaagctgaa
    10081 gtacacgggt caccatgata taacctggta ccaaaccagc gttgaacaac atactgagaa
    10141 ggacgaagat ggtaaaaaat ctgcgatact taaaggttgt ccgtgaaata gcgtaggcat
    10201 aggttgttgt gataaagaca tttgtcaatg tcccaactac ggttacaaag acagagatga
    10261 agagggcttg taggatttta tccttaaact gtgccaaaaa ctcaaaaccg tctaagccaa
    10321 attgggatgg gaagaagcta tagccgtatt ggaggaggct tttctcgtct gtcactgaaa
    10381 taatgataac gaatacaaaa ggtaggatac aagagagggc aatcaaaccc gaaatgatac
    10441 tgaagaagat atctgctttc ttactgaagg agtgaatgcc gacattatca attttttctt
    10501 ttttaatttt cttttttgcc atattctcct cctttctaga acaaagctga gtttggatcg
    10561 actcgtcttg caagcaagtt tgataggata accagaatca aaccaacaac ggattggtaa
    10621 agaccggctg ctgcagccat accgatatct gctgtctgag tcaaaccatt aaaaacatat
    10681 acgtccaaaa cgttggttac attgtaaagc tgaccagcat tgtgtgggat ttgatagaag
    10741 agaccgaagt ctgcgcggaa gatatttccg actgcaagga tggtcaatac agttacaagc
    10801 ggagtcaact gaggaatggt tacgttgcga atacgttgcc acttgctagc tccgtccact
    10861 gtcgctgctt cgtagtaggt tggatcaatt cccatgatcg tcgcatagta catgacactg
    10921 ctatatccaa aacctttcca aatacctagg aaaagtagga gatagggcca gatgcccagg
    10981 tcagcgtaga aattgacttc tttgagacca agactttcca atagatgatt gaacacccct
    11041 ttatcaatat ttaggaaggc atctgtaaag aaactgatga taacccaaga caagaagtaa
    11101 gggaacaaca tagaagtttg gaaaatcttc accattctct tagaacggag ctcgctgagg
    11161 ataatggcaa tccctacaga tacaactaaa cctagaaaga taaagccaag attgtagagg
    11221 acagtatttc gtgtgataat aaaggcgtct cttgaactaa ataagaatct gaaattatcg
    11281 agtccgaccc atttactatt tatgatacta tctatgaaac cattactggt catgtggtag
    11341 tctttgaagg caaccacgtt cccaaatact ggaatgtaaa agaatagaat caaccagagt
    11401 gcccctggca aaaccatcaa gagaaagatc cagttgtctc tcaatgtttt tgaaaacttt
    11461 ttcataattt cctccctttt tattttgata tccatctaaa aattcttttt tagacttttg
    11521 ataacgatta cattattagt atactcctat ttgcaggtta ggttaaactc ctaattatag
    11581 aaaaaactcc acaaattatg tagcagattt aaaactttat caccactatc aaacaaatgt
    11641 cctaaatcaa ttgtttattt tatctctatt agcccagtga tggcgtcact ctgttataag
    11701 catccaacaa cggggtatac tgaaaaatct ccagactagg gaactcagcg atagttccta
    11761 atctggagat ttttaatatg ttattaggcg tttgctttca acttagcaat aacctcttta
    11821 agattatcaa tcaactctgc tgcagtatgc tcagagcctt tttcatctgc caagaacaaa
    11881 actgcttttt gaagttcttc ttgagagttt tcaaggacat ccttatctac tgtttctttg
    11941 cccactgttt gtcctccatc cttagttgct gactggcttt ccttaatgga ctctagggaa
    12001 acaatggcat ctttgactgt ttgcaagata ttacgtaaac cttgctctgt caaactatca
    12061 tctgcaaaag ctttcttagc ctctgccaaa accagacgtg ctgaatctgt ggtaggattc
    12121 gatacacctg tcaatgatct caaaagattt tctaaggttt gagtctgctt actaatacta
    12181 gactaaaatc aaaaagtatt atataacagt gatatgaaat caactaaaga agaaatccaa
    12241 accatcaaaa ca
//
LOCUS       DAVMGZ010000052        12110 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000052 DAVMGZ010000000
VERSION     DAVMGZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12110
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.053"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(82..2322)
                     /locus_tag="VYQ93_001156"
     CDS             complement(82..2322)
                     /locus_tag="VYQ93_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV3335852.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGIFGFWFVFQNWQQSQASQNLADSVEGVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKENVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            complement(2306..2956)
                     /locus_tag="VYQ93_001157"
     CDS             complement(2306..2956)
                     /locus_tag="VYQ93_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV3335853.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVKKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            complement(3024..3593)
                     /locus_tag="VYQ93_001158"
     CDS             complement(3024..3593)
                     /locus_tag="VYQ93_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3335854.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKDRIVTRVHYVLTKEDYFANK"
     gene            3770..4195
                     /locus_tag="VYQ93_001159"
     CDS             3770..4195
                     /locus_tag="VYQ93_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV3335855.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            4196..4609
                     /locus_tag="VYQ93_001160"
     CDS             4196..4609
                     /locus_tag="VYQ93_001160"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV3335856.1"
                     /translation="MGAHFLTKQAGGSGVLLGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLMSNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            4555..4881
                     /locus_tag="VYQ93_001161"
     CDS             4555..4881
                     /locus_tag="VYQ93_001161"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335857.1"
                     /translation="MVDIAVDQGGLIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(5248..5507)
                     /locus_tag="VYQ93_001162"
                     /pseudo
     CDS             complement(5248..5507)
                     /locus_tag="VYQ93_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(5510..6011)
                     /locus_tag="VYQ93_001163"
                     /pseudo
     CDS             complement(5510..6011)
                     /locus_tag="VYQ93_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            6047..6226
                     /locus_tag="VYQ93_001164"
     CDS             6047..6226
                     /locus_tag="VYQ93_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335858.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            complement(6204..7172)
                     /locus_tag="VYQ93_001165"
     CDS             complement(6204..7172)
                     /locus_tag="VYQ93_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV3335859.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(7258..7473)
                     /locus_tag="VYQ93_001166"
     CDS             complement(7258..7473)
                     /locus_tag="VYQ93_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV3335860.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEESAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            complement(7482..8336)
                     /gene="cvfB"
                     /locus_tag="VYQ93_001167"
     CDS             complement(7482..8336)
                     /gene="cvfB"
                     /locus_tag="VYQ93_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV3335861.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPVLKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(8396..8953)
                     /gene="frr"
                     /locus_tag="VYQ93_001168"
     CDS             complement(8396..8953)
                     /gene="frr"
                     /locus_tag="VYQ93_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV3335862.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(8962..9699)
                     /locus_tag="VYQ93_001169"
     CDS             complement(8962..9699)
                     /locus_tag="VYQ93_001169"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV3335863.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(9783..11117)
                     /gene="trmFO"
                     /locus_tag="VYQ93_001170"
     CDS             complement(9783..11117)
                     /gene="trmFO"
                     /locus_tag="VYQ93_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV3335864.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(11412..11666)
                     /locus_tag="VYQ93_001171"
     CDS             complement(11412..11666)
                     /locus_tag="VYQ93_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV3335865.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            11661..12110
                     /locus_tag="VYQ93_001172"
                     /pseudo
     CDS             11661..12110
                     /locus_tag="VYQ93_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3453 a         2763 c         2316 g         3578 t
ORIGIN      
        1 tacgttttca ttataccatt tttatcatta ttatgcaaca aatttttagt ttatttcact
       61 aatctacaac atttatcctt cctatcgaac actttcgatt ttccaactat ccaacccctt
      121 aaaacgtata gctccttgct ggtcagttcg ataaactttg ctattgatac cttccagtcg
      181 tgtcaatgtt tcctgatggg ggagtttcat tcgattgctc tttccaactg agataagagt
      241 aagctctggt ttgagttttt ctagaaaggc tggacttgat gattttttat tgccatgttg
      301 gctagctttc aaaacattta ctttcaagtc tggatagtgc ttcagcaagt ccttctctcc
      361 tttctcctcc aaatttcccg tgaagagaaa ttgcttatcc aagaatttcc catacagaac
      421 tagggtatca tcgtgtcctc catctcccat tttccttgga gatagaactt ctaactgact
      481 tccaaaaatg ggcaagttct cccctactgt catactacgc acctttgttt gagtcgcctg
      541 tagttctgcc acaaattcct tctgtttcag actgtctttt gatactagaa tctcccctac
      601 atggaaagcc ttggtcacct ctgacaaatc tccaacattc tccttgtccg tgttagtcaa
      661 aattagctgg tcaatcttag ctactcctcg acttttgaga tagggaatca aggttcgctg
      721 ggcattgctg gtcgtcatct tttcttgcca tttttcgatt ttcttataag attctgcctt
      781 accacctaca tctatgagaa tggttttccc agttacatcc cgtaggaaaa tactttctcc
      841 ttgccccaca tccagcatgg tgatttcatt ttccagtgga tgcttggtca ggagaaagag
      901 ccctgtaatc aataagcaca ataccgttag ctttttaatg ttttttctca aatcatagac
      961 caaagccaag gaaattaaca ataggattaa aaaccatgca ttgggttgtc caaagaccag
     1021 aggtctactt gtcacctgtg acaccaagcg aataatgccc tccaaccatt caaaaataaa
     1081 gttcagctga atgactggat agagaaagga aaggacaaat aaaatagaca agagcggtaa
     1141 gaaggtcaag tcaaatagaa aggaaaagac aaaggtcaaa aggatagacc aaggttgaaa
     1201 ttccgcaaaa tagaaggata gaatgggcaa tatgcccaag gagatgacta gactttcact
     1261 agtaacagcc ttgagcccct ccccttcttt gctggtcatg gtcaggataa aagcataagc
     1321 gcaggacaag actcctcctg ctgtcaagaa aaagtttggc atgacaataa agaggacaag
     1381 caccgtcaag gcaaaattat ccaagccctt aaccccatgt tgagccagta gcttttgcaa
     1441 gagactgcga ataaccgatg ctgaaaatcc agttagtccc gcatagataa gggaaaaggg
     1501 ataagtcagc catttcaact tttcttgggt caagcccaat cgcaagagaa gtttcttaaa
     1561 tccattcatg aaaaaaccta cctgcatacc agatagggca aagaggtgga taattcctag
     1621 actggaataa agctcattca tctcctcaaa gtcggtgtcc agatgtccca gcaagagtcc
     1681 tgtcatgtaa ttgcgcatag ggtctggaaa gtgcgtctta atccaaacca cagcctttcg
     1741 acgtaaactg gacaagtttt ctcctatatc ccaactgcca atcttttgaa gtgactggat
     1801 tgttttgata ttgagagtct ggtaaattcc ctgagtcttc agataggctt ggtaattaaa
     1861 gccaccaaaa tttctctgcc cttctggctc cgaaagcttc ccttctagtc ctatctcatg
     1921 caggtcggtt aaagcttgaa aggcttcttt ctcctcctcg gactggagtt tataatagac
     1981 ttggaaagca cgaccgttag acttgccacg aaaggataga ctatcaccat taaccttaat
     2041 agtatcaggc aaaatccgta ccccttcaac agaatccgcc agattttgac tcgcttgact
     2101 ctgttgccaa ttttgaaaaa caaaccaaaa tccaaagatt ccacaaatca ctagaacttt
     2161 accagcagat ttccacggaa attggataaa gagacagact agcagaaaaa caaagcccaa
     2221 caaagcaaga taagatgctg agaaaatagc gtaataaagc caaagtaata gaaaactcag
     2281 gtaaattagg ggaatagaga aattcttaat ccactgtaac atagtcttta agcttttcta
     2341 ttgttttgcc accaatgcca gagaccttct tgagttcgtc tactgacttg aacttgccat
     2401 ttgcctcacg atggtcgata atgtcctgag ctcgttttcc tcccagtccc ttgacctgct
     2461 tgagttcttc cagactggcc ttgttgagat tgaccttctt ttccttgctt attgaagaag
     2521 ctgtccccaa accagtctgt tggctaactg cttcttctcc cttagtagga acgtaaacca
     2581 gagcctcatc actaactttc tgagctagat tgagcgactt gctgtctgct tgctctgtca
     2641 agccaccagc cttctgaaca gcatcattga ctcgactacc tacaggcaag tcataaatcc
     2701 ctggcgattt gacagcacct ttgacatcta ctgtgattag atcttgttca aggggttctt
     2761 ccttctcttc cttcttcact tccttttcgg ataccaaatc cttggaaaca gctgcaactt
     2821 cagcctgcaa attcgtttct ttgacaggtg tttgtggagc tggctttagc aggaaaaatc
     2881 cgcctacaag caagcccaga ccagtacaga tgacgatgat tttatactct ttgattttct
     2941 cgataattgc ttccatattt tctcctctct tagattattc gtaagatgaa gaaaaaacag
     3001 tcgaaaaatt tcttttcaac tgcttattta tttgcaaaat agtcttcctt ggtcaagaca
     3061 taatgaactc ttgtaacgat tcgatctttt tcatgctggt ccatacaagc atatggttct
     3121 gcatgggaaa aacgcatgcc tgatttctcc atgacctttc ctgacgcagg attgtcctta
     3181 tcgtgaaggg cagtcaactt attcatccct atcttctcaa aagctagctc aatcacagca
     3241 cgattggctt ctgtcgttaa tccttgattc caatactttt tattgataat gtagccaata
     3301 gctgccttct taagaacaga atcaatcttg tgcaagtcaa tggttccaat aaactgacca
     3361 ttgcttttta gttctattcc ccaacgtccc aagggattag ccaaatagaa ctgagcaatg
     3421 ttattcttgg tttcttccaa gctttgattg gttggaaaag tgtaacgtgt attacccttg
     3481 tccgaggcat agtcaaacat tgcttccgca tcatccaaag ttacaggtct aagcaataaa
     3541 cgctccgttt ctatagacgg atactgggca agttttacaa aaattgattc catacttttc
     3601 caaccaatca aagcttgatt ttcactagtc tagcataaaa aatttggatg acaagttttt
     3661 cttgattttt gaagcggttt catatacaat gaaaccgtca catttttgat ttatgaactg
     3721 gaatgtgaca accaaaacaa gaccgtcaat tgaaaggtag gttatctcaa tgttaatcgg
     3781 aatcccaaaa gaaattaaaa ataacgaaaa ccgtgtcgcc ctcacacctg caggtgttca
     3841 tagcttagtt agtcgtggtc atcgtgtcct tatcgaaaca aatgctggtc tcggttctgg
     3901 ctttactgat gctgactatc aaaagcaagg agctgagatt gtcgctactg ctggtgaagc
     3961 ttgggcagca gagttggttg tgaaagtaaa agaaccttta agttctgaat acggttactt
     4021 gcgcgacgat cttcttctct tcacctactt gcacatggcc gctgctccag aattagcaga
     4081 tgctatgtta gcagcaaaaa caacaggaat tgcctatgaa actgttcgtg acaatcaagg
     4141 acaactaccg ctcctcgttc ctatgagtga ggttgcaggt cgtatggctg tttaaatcgg
     4201 agctcacttc cttactaagc aagctggtgg ctctggtgtt ctacttggtg gtgtaccagg
     4261 tgttccaaaa ggaaaagtaa ctatcatcgg tgatggtgtc gtcggtacac atgctgcccg
     4321 catcgccctt ggtcttggtg ctcaagtgac tattttagat attagtgcca agcgtctctc
     4381 agttctagaa gaagtctttg gaagtcaaat tcaaactctt atgtctaatt cattcaacat
     4441 tgaagcaagt gtgagagatg ctgatgtggt gattggagcc attctcatcc ctggtgcaaa
     4501 agcaccggaa ttggtgacag atgagatggt caaacaaatg cgtccaggct ctgtatcgtt
     4561 gacattgctg ttgaccaagg tggccttatc gaaacagctg accgtgtgac aacgcacgat
     4621 gaacccgtct atgaaaaaca cggtgttctc cactatgccg ttgccaatat ccctggtgcg
     4681 gttgcccgca cttcaaccat cgccctaacc aatgtcactc ttccttatat cgaagctttg
     4741 gctggcaaag gattcgcaca agcaatctct gaagatgaag gcttgcgtca aggtgtgacc
     4801 acttatcaag gttacttgac tagcctacca gttgctcaag gacttaatcg tgactacact
     4861 gatatcaatg atttagtata aattttgaaa agaaaagggt gacttcgcaa gtcctaccca
     4921 ctgtgctttg tgtcatcagc tattacatcg ttggtttatt gtactgtata ctttgcaact
     4981 ggagactttc gttccagact caattgtgca taagaaactc taaattgaat aaagtcaaca
     5041 gagtttcgcc caaccacttc gtcttgtaat gacaattata taaaccaaag gaagctgagc
     5101 tctttagtcc cagcttcttt ttatatataa aattttaccc gtgcaaagac agggccttag
     5161 cagacttctt ttttacttcg ttcacccttg ctttttcttt ttaggtttgg gcgttggcag
     5221 ttggttatac atagctaaaa tcaggtctta tagaaacatc ttattatcaa gttcttccac
     5281 tcaaatcatt tctttggcac ctttgtatgg aaactcaaaa gaagattggt caatcttatc
     5341 taagactgct tgcacgggtt taactaaaag cgatcgtcat aaatgccgcc aataatcttg
     5401 ccgcggaagt aaagaatata ctcccccatc atggaacggt aagtcacatc atctaatcct
     5461 gataattgtt ccaaaacaaa ttccaaatag ttcttacttg atgccatttc taatcttcta
     5521 ggctctgttc aacgataaca accgtataga gttctttctt aacctcgcat ccaattgatt
     5581 taaagccctg cttttcccaa aaatgctgag attgcggatt tcccttaaca taagccaaac
     5641 gtgcctttcg aaagttctta gcaaaataag ctagtgcttc tgtcacaata tgactaccaa
     5701 tccctttcct ctgataggct ggatcaacca taaacaaacc aataaaaaca gtctcctcat
     5761 caggatatgc atagacaaaa tccataacag ccacaaggtc agatccattc caaaatccaa
     5821 caaaaaaact tatcagcctt agctttacct tcaggtagac aaagcatgtc ctcttttaca
     5881 gttgcaaaat ttggctctgg tggacaatgc tgaaaataca gaggattact ttcatataaa
     5941 gataaaatac ttggaatatc cttttcagtt agtatcctac aactgtaata cttagatagt
     6001 tggtcaatca tcttttcaaa ttcctacttt cttgtgccct gtgattatga cacaggaaga
     6061 tgcactgatc gtcatcagcc acataaaagt tctttccatc gtgcctaatc gttgtctcaa
     6121 acctttggat aaaaccttta gcctatacaa ctggattttc ctctctcaaa agtatattct
     6181 tttgcaggcg aacttcctca aaatcagtcg tgtgcaactt cagtagaata ttcataggct
     6241 cggataatct gagcgacaac tggatggcga accacatcct tggctgaaaa atgaacaaag
     6301 tcaatctgat ggatgttctt gagtttctct tgagcatcaa tcaaaccgga cttgacatta
     6361 cgtggcaggt caatctgact gatatctcca ttgacaatca tcttagaatg aaagcctaaa
     6421 cgcgtcaaga acatcttcat ctgcatgatg gtcgtgtttt gcgcctcatc gagaatgaca
     6481 aaggcatcat ccaaggtccg gccacgcata taggcaaggg gcgcaatttc gataatttca
     6541 cgctccatga gacgagtcgt ttggtctttc ccaagaattt gatacaaggc atcgtaaaca
     6601 ggacgaaggt aaggatccac cttctcctta agatcacccg gaagaaaacc tagactctct
     6661 cctgcttcca ccgctgggcg tgtcaggata attcgtttga cttgcccacg tttaagggca
     6721 gtcactgcca aggtcactgc aaggaaggtc ttccctgtcc ctgcaggtcc gattccaaag
     6781 gtcacatcat gctgtttgac actgtccaca taaagttttt gacccaaggt tttgactcgg
     6841 ataggcttcc cagtattgtc tttgatgatt tcttcttcgt aaagggcgac aaacttgtca
     6901 atttcatcat ttttgaccat gctaatcgca gtgactacat ctggcgtacc aacggtcatc
     6961 ccacgattta ccaagaccat caaagcctga ataacttgac gggcttcctc acaggcagac
     7021 tcttctccca aaacctggac aatctccgta cgagcatgaa tcacaacatc aagctcttct
     7081 tccatcaaac gaagatggcg ttcattggaa ccaaaaagat gaaacaggtc atctggatga
     7141 ctcagttgaa tgtctattga atgttccttc aaataaagaa cctctctaat ccttttattt
     7201 ctttttatta tagcagaggg atggagaaat tactagccca aacccaatga ataaatgcta
     7261 gtgttctaga tattcctgcc aaatgctgtc aaaatctcct aggttaaaag agaaactggc
     7321 atgctcctcc aaaaagcgac tgacctcatc aaaatcatct gtatgttttg gaaaggctga
     7381 ctcttcaaaa gcgaggtctg ccaaaattgc tttgggactg ttacttttag gattgcgctc
     7441 ggtcatgagc caagtgtaaa atgattttct cataagcctc cctaaatcaa ctctgtccca
     7501 aactggtctt gcttgatttt accagccttc ataagaccac ctaaagcttt cttgaactga
     7561 cctttagaaa tgccaaaggt tgccttgatg tcgtctggag atgacttgtc atttaaggtc
     7621 atgaaaccgc cattgctttc caaataagtc aaaatcatct gagcatcgtt ttccaacatt
     7681 tcaaaggagc gtggtttgag ggagaggttc agagtgcggt ccacttcacg gaaaccaata
     7741 acgcgcgcat ctaatacttg ccccaaacgt ggctctgcgt aacgctcgct aggatgaata
     7801 aaaccaagca tattattttc tggtaggtaa acaaaagttc ctgacagctt gagacggtaa
     7861 acaatggctg gccagttttg gttctgcatg ttgttgtagg caggacgagc gagacgttgg
     7921 aagtcttctt gataagccaa gaggccccag atacggtctt tcttatccac ttcaagacgg
     7981 atgtagagtt ggtcgccctt cttaggccag agttccttga gcacagggag aatatcgagt
     8041 gacacaacga tttccttgtc aggaaggcct gtatccacaa agacacccaa gtccttacga
     8101 acctctgtga cacgtcccca accaaattgg tcctgagtgg cagtcacttc taaggttgtc
     8161 aggcggagtt tttgcttcat atccgtgtat gcaaaacctt tgaccgtatc ccctactgta
     8221 tgttggccct cttccttagc aagagcatag gtttgaccat ccttttgcac aaagtaaaaa
     8281 cggtcatttt catcgatgat cagtccaacg ataaaacttg caagatttgt attcatattt
     8341 ccttctttcg aataaaactc agccagcaat gccaactgag tttttctgtt tatttttaga
     8401 cttccaaaag ttctttctct ttgttagcag tcatgtcgtc gatgtgttta acagcatcgt
     8461 ctgttacttt ttgaatatct ttttcaagag tcttcaattc gtcttcagtg atttcttttg
     8521 ctttttcttg cttcttagct tcgtccatag catcgcgacg gatattgcgg acagccactt
     8581 tagcattttc gccgaccttc ttcacttctt tagcaaggtc acgacgagtt tcttctgtaa
     8641 gagctgggat aaccaagcga atcacagaac cgtcattagc cggtgtgata ccaagatcag
     8701 aagcgttcaa ggcacgttcg atgtctttca atgaagactt gtcaaatggt gttaccaaca
     8761 aaacacgcgc ttctggaatc gtaattgaag cgatttggtt aagaggagtt tcgactccat
     8821 agtattctac atgtacacgg tcaagcaagc ttgcattggc acgaccagca cggataccac
     8881 caaattcacg agcaagtgat tggtgagact gggtcattct ctctttagct ttttcaataa
     8941 ttacgttagc catattcttt cttattcctt ttcttcgata ttatttgaaa ctgttgttcc
     9001 gatattttca ccaaatacaa cacgtttgat gttgcctgat tggttcatgt tgaagacaac
     9061 caagtcaatg tcgttgtcca ttgagagggt tgaggctgtt gagtccatga tacgaagacc
     9121 tttgttgata acatcacggt gggtcaattc ttcaaactta acggctgtct tgtccttctt
     9181 aggatcggca ttgtacacac catcgacgcc atttttagcc atgaggatgg catctgcttc
     9241 gatttcagct gcacgaaggg ccgctgttgt atctgtcgag aagtatggtg aaccaattcc
     9301 agcaccaaag ataacgatac ggcctttttc aaggtgacga agggcacgtc cacggacata
     9361 aggctctgcc acttgttgca tagcaatagc tgtttgtaca cgcgtatcaa ccccaacttg
     9421 ttgcaatgaa tctgccatca caagagcatt cataacagtc ccaagcattc cagtgtaatc
     9481 tgcctgaaca cggtccatac ctgcttctgc tgcaggttct ccacgccaga gatttcctcc
     9541 accaataaca agggcaattt cgatacctaa gctatgaact tcttgaatct cttttgcgat
     9601 tgtttgaact gtttggatat caatccctac gccacgttca ccggcaaggg cttcacctga
     9661 taacttgatt aaaatacgtt tatacttggg attcgccatt ttcactctcc ttctttcatc
     9721 ctacctattt tatcacaatt tctaagattt ttatagtatc atgaacaatt ctttcaaaaa
     9781 aattagacag tcaaaaattc ctctaagtcg gcaagggcac gctctgcaat tttttcataa
     9841 cgagccttct tatcacggat acgctcgcct tccaactcct tgatgatccc aaaattgaca
     9901 ttcattggtt ggaaatgttt gctgtcggca tgggtaatgt aatgagctaa gcttccaatc
     9961 gctgtcgtct cggggaaaat aacctcgctt tcttccttga agagacgagc tgcgttaatt
    10021 cccgcaacta agcccgaagc cgccgactca acatagcctt ccacccccgt catctgacca
    10081 gcaaagaaga gatttggttg tttcttggaa cggtaagtct gctcaagaag atttggtgaa
    10141 tccatgtaag aattgcgatg catgacacca taacggacaa actccgcatt ttcaagaccc
    10201 ggaatcattt ggaagacacg cttttgttct ccccatttga ggtgggtctg gaaaccaaca
    10261 atattgtata ggctaccagc tgcattatcc tgacgaagtt gcacaaccgc ataaggtgtt
    10321 ttaaattctc catcacgagg tcctgtgtag tcgtccggat actcaagacc gactggcttc
    10381 atagggccat aaagcatagt tttaatgcca cgtttggcca taacttcgat tggcatacat
    10441 ccttcaaagt acttttcttt ttcaaaagaa ttgagcggtg cttcttccgc attgaccaag
    10501 gcttcatgga aatccataaa ctcttgcttg gtcattggag cattgagata ggctgcttct
    10561 cccttatcat aacgagattt gagatagacc ttactcatat cgatagtgtt gacatcgata
    10621 ataggcgctg ctgcatcgta gaaatagaag ccatcgccat tattaagggc atgaatcttt
    10681 tcagccaggg catcgctagt caagggacca gtcgccacaa ctgtaataac atctgttggc
    10741 aattctgtaa tttcatcacg caccacttca atcaaggggt ggttgaccac tttttcggtc
    10801 accatttgag agaaaccatc acgatccacc gcaagggctc caccggcagg aacacgtgta
    10861 gcctcagcag attccaagat aacagaaccc aagcgacgca tttcttcctt gagaagacca
    10921 actgcatttg tcaaagcatc cccacgcaaa gaattggaac aaaccaactc agcaaaattg
    10981 tctgttttat gctggggtgt agacttgaca ccacgcattt cataaagttt aactggaata
    11041 ccacgctctg cgatttggta agctgcttca gaacctgcca aaccagcacc gataacattg
    11101 atataagatt gagacacgac actaatacct ctttgggagt gtgaagttaa gattcacatt
    11161 gaaaaagcca atcagactta cgagatttcg agtttcttgg ctcaggctga aaaagtcccc
    11221 cagactttcc tcgctctcga atttcttggc tcaagctgaa aaagtccccc ggactttttc
    11281 actcccacaa atctttctat ttttttcttc tactagtata acaaaaaaag ggaagaaggc
    11341 aaacttccct gtttagtcat tttcttgatg taaagagata gagagtattc cagttaagaa
    11401 tcaattatat gctactctat aaaatctttt ccacataacg gatcgatagg gactgttatt
    11461 ctatctcttg ctataaccgt attatctaaa acagcataac attcaacata gtggtctcct
    11521 ttaaactgag aatcttcgtg atgttttatt ttacctgaaa aaatagcacc acgctcacaa
    11581 tttcttctaa tcgcctcagc ttcaatattt cttactttcc aatagacttc ctgcgaaaca
    11641 aaatatggta tagtagttct atgaatgatg aagcaagtaa acaactaact gatgcacgat
    11701 ttaagcgtct tgttggtgtt cagcgcacga cttttgaaga gatattagct gtattaaaaa
    11761 cagcttatca acttaaacac gcaaaaggtg gacgaaaacc taaattaagc ctagaagacc
    11821 ttcttatggc cactcttcaa tatgtgcgag aatatcgaac ttatgagcaa attgcggctg
    11881 attttggtat tcacgaaagc aacttaatcc gtcggagcca atgggttgaa gtaactcttg
    11941 ttcaaagtgg tgttacgatt tcaagaactc ctctcagttc tgaggacacg ataatgattg
    12001 atgtgacgga agtaaaaatc aatcgcccta aaaaaagaat tagcgaatta ttctggtaaa
    12061 aagaaatgct acgctatgaa ggctcaagcg attgtcacaa gtcaagggag
//
LOCUS       DAVMGZ010000053        11779 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000053 DAVMGZ010000000
VERSION     DAVMGZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.054"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            43..630
                     /locus_tag="VYQ93_001173"
     CDS             43..630
                     /locus_tag="VYQ93_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV3335866.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            557..2200
                     /locus_tag="VYQ93_001174"
     CDS             557..2200
                     /locus_tag="VYQ93_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV3335867.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLVQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2211..3572
                     /locus_tag="VYQ93_001175"
     CDS             2211..3572
                     /locus_tag="VYQ93_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV3335868.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            3629..3757
                     /gene="blpC"
                     /locus_tag="VYQ93_001176"
     CDS             3629..3757
                     /gene="blpC"
                     /locus_tag="VYQ93_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV3335869.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(3828..5168)
                     /locus_tag="VYQ93_001177"
     CDS             complement(3828..5168)
                     /locus_tag="VYQ93_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3335870.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(5182..5919)
                     /locus_tag="VYQ93_001178"
     CDS             complement(5182..5919)
                     /locus_tag="VYQ93_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3335871.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(5924..6262)
                     /locus_tag="VYQ93_001179"
     CDS             complement(5924..6262)
                     /locus_tag="VYQ93_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3335872.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            6538..6867
                     /locus_tag="VYQ93_001180"
     CDS             6538..6867
                     /locus_tag="VYQ93_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV3335873.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7035..8084)
                     /locus_tag="VYQ93_001181"
     CDS             complement(7035..8084)
                     /locus_tag="VYQ93_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3335874.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8081..8812)
                     /locus_tag="VYQ93_001182"
     CDS             complement(8081..8812)
                     /locus_tag="VYQ93_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3335875.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVADPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            8880..9290
                     /locus_tag="VYQ93_001183"
     CDS             8880..9290
                     /locus_tag="VYQ93_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV3335876.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9300..9587
                     /locus_tag="VYQ93_001184"
     CDS             9300..9587
                     /locus_tag="VYQ93_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335877.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            9749..9943
                     /locus_tag="VYQ93_001185"
     CDS             9749..9943
                     /locus_tag="VYQ93_001185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335878.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSNHTHD
                     KPTLTWFEEIFEEYHSPFHN"
     gene            complement(10277..11413)
                     /gene="dnaJ"
                     /locus_tag="VYQ93_001186"
     CDS             complement(10277..11413)
                     /gene="dnaJ"
                     /locus_tag="VYQ93_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV3335879.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
BASE COUNT         3813 a         2558 c         2108 g         3300 t
ORIGIN      
        1 tatgacataa tagacctatc cactatatga aaggaattgc caatgacttc ttataaacgt
       61 acatttgttc ctcaaataga tgcgagagac tgtggtgtcg ctgccttagc ctcgattgct
      121 aaattctatg gttcagattt ttctctagct cacttgagag aacttgcaaa gaccaataaa
      181 gaagggacga ctgctcttgg cattgtaaaa gccgctgatg aaatgggctt tgaaacaaga
      241 cctgttcaag cagataaaac gctctttgac atgagtgatg tcccctatcc atttatcgtt
      301 cacgttaaca aagaaggaaa actccaacat tactatgttg tctatcaaac aaagaaagac
      361 tatctgatta ttggtgatcc tgacccttct gtaaaaatca ctaaaatgtc aaaagaacgc
      421 tttttctatg aatggactgg agtagctatt tttctagcta ccaaacccag ctatcaaccc
      481 cataaagata aaaagaatgg tctactaagc aagcttcctt cctctgattt tcaaacaaaa
      541 atctctcatt gcttacattg ttctctcaag cttattggtc actattatca atataggtgg
      601 ttcttactat ctccaaggaa tcttggatga atacattcca aatcagatga aatcaacttt
      661 aggaatcatc tcagttggtc tggttatcac ctatatcctc caacaagtca tgagcttctc
      721 cagagattat ctcctaaccg ttctgagtca gagattaagt attgatgtga ttttatccta
      781 tattcgccat atttttgaac ttcccatgtc tttctttgcg acacgtcgta caggagaaat
      841 catttcacga ttcacagatg ctaactctat tatagatgcc ttggcttcta ccattctttc
      901 tctttttctg gatgtttcta ttctgattct tgtaggaggc gtcttactgg tacaaaaccc
      961 taatctcttc cttctttctc ttatttccat tcctatatac atgttcatca tcttttcttt
     1021 tatgaaacct ttcgaaaaaa tgaaccatga tgtcatgcaa agtaattcta tggttagctc
     1081 tgccattatc gaagatatca acgggattga aactataaag tcgctcacga gtgaagaaaa
     1141 tcgctatcaa aatatagaca gcgaatttgt agattatttg gaaaaatcct ttaagctcag
     1201 taaatattct attttacaaa cgagtttaaa gcagggaaca aaattagttc tgaatatcct
     1261 tatcctatgg tttggcgctc aattagtcat gtcgagtaaa atttctatcg gtcagctgat
     1321 tacctttaac acactttttt cttactttac aactcctatg gaaaatatta tcaacctcca
     1381 aaccaaactc caatctgcga aggtcgctaa taaccgtttg aacgaagtct atctagtcga
     1441 atctgaattt caagttcaag aaaaccctgt ccattcacat tttttgatgg gcgatattga
     1501 atttgatgac ctttcttata agtatggttt tggacgagat accttaacag atattaatct
     1561 cacgattaaa caaggagata aggttagcct agttggagtt agtggttctg gtaaaacaac
     1621 tttagccaaa atgattgtca atttctttga accctacaaa ggacatattt ctatcaatca
     1681 tcaggatatt aaaaacattg ataaaaaagt cttgcgccgt catattaatt acctaccaca
     1741 acaagcctat atctttaatg gctctatctt ggaaaactta actttgggcg gtaatcatat
     1801 gattagccaa gaagatattc taagagcttg tgaattagct gaaattcgtc aagacattga
     1861 aagaatgcct atgggctatc aaactcagct ctctgatgga gctggtctat caggaggaca
     1921 aaagcaacga atagccctcg ctcgtgctct tttaactaaa gctcctgttt taatactaga
     1981 cgaagctact agcggtcttg atgtcttgac tgagaaaaag gttatagata atcttatgtc
     2041 tctaactgat aaaaccattc tctttgtagc ccatcgtctc agtatagccg aacgaaccaa
     2101 ccgtgtcatt gttcttgacc aggggaaaat cattgaagtt ggtagtcacc aagagttaat
     2161 gcaggcgcaa ggtttctacc atcatctatt caataaataa ggagaatgtc atgaatccta
     2221 atctttttag aagcgtcgag ttttatcaga gacgttacca taactatgcg acagtgttca
     2281 ttatacctct ttcattacta tttactttca tcttgatttt ctcccttgtt gccacaaaag
     2341 aaattactgt tacttcccaa ggagaaatcg cccctacaag tgtcattgcc tccattcagt
     2401 caaccagtga taatcctatc ctagctaatc atttagtggc aaatcaagta gttgaaaaag
     2461 gggacttact catcaaatac tctgaaacaa tggaagaaag tcagaaaact gccttagcaa
     2521 ctcaattaca aagacttgag aagcaaaaag aaggacttgg aattttgaaa caaagcttag
     2581 aaaaagcgac tgatcttttt tctggcgagg atgaatttgg ctaccataat acctttatga
     2641 attttactaa acaatcccat gatattgaac tgggtatcac aaagactaac accgaagttt
     2701 caaatcaagc taatctttcc aatagcagtt catcagctat tgaacaagaa attacaaaag
     2761 ttcaacaaca aattggagaa tatcaagagt tgagagatgc tatcataaat aacagagcac
     2821 gcttaccaac tggcaatccg caccagtcaa ttttgaatcg ttatcttgta gcctcacaag
     2881 gacaaacaca aggaactgca gaggagccat ttttatctca aattaatcaa agtattgcag
     2941 gtcttgaatc atctatcgca agcctcaaaa ttcagcaagc tggtatcgga agtgtagcaa
     3001 cttatgataa cagtttagca accaaaattg aagtactccg cactcagttt ttacagacag
     3061 cctcacagca acaactaact gtggagaatc aattaacaga attaaaagta caactagatc
     3121 aagccacaca gcgtttggaa aacaatacct taacctcccc aagtaaaggt atcgttcatc
     3181 tgaacagcga atttgaaggt aaaaatagaa ttccaactgg tacagaaatt gctcaaatat
     3241 tccctgtcat cacagataca agagaagtac taatcactta ctacgtatct tctgactatc
     3301 tacctctact agataaagga caaactgtaa gattaaaact ggagaagatt ggaaatcacg
     3361 gcaccaccat catcggccaa cttcagacaa ttgatcaaac tcctaccaga acagagcaag
     3421 gaaatctctt taaattaacc gctcttgcaa aactatctaa cgaggatagt aaactcatcc
     3481 aatatggctt acaaggtcgc gtcactagtg taactacaaa gaaaacatat tttgattatt
     3541 tcaaagataa aattttaacc cattctgatt aattttcaga taacactcta taactattta
     3601 ttatcttatc aaaaaggaga atcataacat ggataagaaa caaaacctaa cttcatttca
     3661 agaactaaca actaccgaac tcaaccaaat tacaggtgga ggattgtggg aagatttatt
     3721 atataacatt aatagatatg ctcattacat cacataagaa cttcatcatc caatacaact
     3781 ataaaaaaat aagaccgaga aacaagtact ctcggtctta tttttcatca ttctgtatgt
     3841 atcacagtaa gtacctgacg aaagacttga ttttgacagg tggtatttag actggtatta
     3901 ggatggcttt ccacaatctt catgacggta tagagaccaa ctcctctctc ctccccttta
     3961 gaactggctc caaaggagaa gatttcagaa atatcgatgc cctcttcttt gatggagttt
     4021 tcgatgataa aggtctcctg tgctccattt tttaaaaagg cgattgaaac atgaggttga
     4081 ctagcttcca cactggcttc aatagcattg tcacaaagga tagacacaat ggttagaaaa
     4141 tcaagtagac tcatcccctc gacctgaatc tcctcaggaa cttcgacatt aaagacaatg
     4201 ttcttatctc tggcttttaa aaatttccct gctagaagac ttttgagggc tttatcacga
     4261 atatttacca atctgcccag gtcatattta ttgttctgca atttctgact ggaatccttt
     4321 aagacggagc catagacctc ttttatctgc tccatatcct cctcttcaat gcccagacgt
     4381 aagctagtca agaggttggt ataatcatga cgaaagctcc gtacttcctt gtaaagctcc
     4441 tctatatgcc gactatagcg ttccatatct ctatagcgca gggcctgctc ttgttccaat
     4501 ctctcataga gtttttcctt caaataggta tccaatttct tgataacccc cataaaaaag
     4561 agtaggtaaa agactaggat gagatggcga acagtctttg attgaatact ttgttcatat
     4621 tcaaaaaaag acagactttc catgactaga tagtagccac ccattatcca gttaatctga
     4681 gtcagggact tttgaaaggc tttatcgaga atctcctttc tcaagctagt aaaatcgtag
     4741 tccaaccatt tcaaaaaagc tagagaaatg aagaaattga aaattattat acataaccca
     4801 gtaaatgagt agccatcata tacttgccct tgtcccaaaa atggaagcac aaaataggag
     4861 actcctctat aaaagagatt caccaatatc attggaaaga gaccataaaa gaaaaggagt
     4921 tttttaggaa gccctctcaa taataagaaa gataagccta tgccgtacaa gggttccata
     4981 aaataagata ggtaaacatt tcctactata tagctaatca tcacaaaaac aaaggccaac
     5041 agtatcttaa aaagaaaggc cttaaaaatc ctctcgaaag taagatcaat tccatccacc
     5101 ttaaagaaga tgacaatttc tagtccatta gtaacaagtg tatacaacaa tatccaagca
     5161 atgttcataa attctcctag ctcagtgtaa tttattgatg gcctcagaca cttccctgac
     5221 cttataacgg gcgattagac aacttccacc attgggaaag aaaagcagtt tttctttctt
     5281 atccaaatgc accacatttg caggattgat gagaaaagag cggtggcact gcaagagacg
     5341 gggctcctgc ttgaaaacct cctctaaact cgctgtaaat tccagcctgt ctgtcttggt
     5401 atagagaata acacgatggg ctctgggcga cgtttcgaga tagtaaacct ctttaaaagg
     5461 atactgaaat tgggcaaatt ttgatttaaa gtaaaagcaa tcttccgcca gacttttact
     5521 atcttgacta ttggcataga ggagggctgt ctcgatccga gattcaaact cctctgctga
     5581 caaggcctta tcaatgtagt ccaaagcaga cacttggtag cgaaaagaca ggggcataaa
     5641 ctccgagtga gtcgtcacaa agacaatcag ggcataagga tcccgatccc gaatctttct
     5701 agccacttcc agtcccttca tctcttcatt tcgaatctca atatccaaaa agaatagctg
     5761 atgggccccc ttctcatgca cttcagccag cagttggtcc ggcttgccaa atacctcaaa
     5821 agagctagga atgatatgat gtgctttcaa aagtttctca atcgtcgttt caattctagt
     5881 ctgttgggaa aaatcatcct ctaaaacaaa tattctcatc ttcttactct ccctctttca
     5941 accatttttg acgaatttct ctatagcgac gtctggcata cttgacagcg tattgacctt
     6001 cttctccgag aaaaaggatt ctttcttgaa aatcaatcga ttttaattta tcaagattaa
     6061 ccaaacaatt tcgatgacat ctcatcaagg tttccccaca ttggttctca agattactta
     6121 aattttgaag catattaaaa ctagcttctt ctgtaacaat ctgtacggta tgggctttag
     6181 ttggatgtgt ttggatatag tagatatcgt cgatgtttac tttatagact gttttctgcg
     6241 tctgaataat catatatttc attggattct cctcctaaga aaagaataac agaaaaggtc
     6301 tagcccaagc acacccaatc ctaaacagtc tttgaaaact cctaaaagga ttattttcta
     6361 caacatcatg atgagcttac aactatttta tcataaaatt cactcagcgc aattcaagac
     6421 atttcaatga caattaaaga tttgaataaa aaaaaagaat taaaagtgat aaactaacag
     6481 tatcaaaaca cagttgccag aagtaagact ggcacccaga ttaaaggagg cattcccatg
     6541 acagatacag accccatcaa aagagctcat actttgatta ctgacttaaa caaagcctac
     6601 caagcatgca aacaggcaag cgctgacgac gtccgctttc aggagcaatt aaactctatt
     6661 cttggttttc tagccaaggc tgaaacagtg gataatcgat tcttgattga actagaaaaa
     6721 ttttaccaga cttccagtct tctcatggga ctcagcgctc ttgatccaga tgctccaact
     6781 cgcgccgctt ggcgggccta tgaccgcttc cactttgacc aagtcaagac caagttaata
     6841 ctcaatgaaa atcaaagagc aaactaggaa gctagccgca agctgtactt gagtacggta
     6901 agacgacgct gacgtggttt gaagagattt tcgaagagta taagtctcta cggaccaacc
     6961 attatcctgt agtaacaaaa aagaagttga gacagactga actcaacttc ttttttagtg
     7021 tcgtatcagc aatgttagtc ctgcatctga cgttttacct gataaggcaa atacaagact
     7081 agtaaaacca aaccagctcc tagtaaggct agaagggcta gtttttcttg gaaggtaatc
     7141 aacccaacaa ctaattccac aagtaaaaca aaactggtca atcctcgaac tacctcctgt
     7201 aagccttttt ccttttgccc cttgtccagc ggaaagagtt gggtcaaata ctggtagtca
     7261 aaggcatgat agagggccag caactggaag agcaagaggt agttaaagag aactaccact
     7321 gctgtcgcaa tccaagcttg ctcgataaaa acctgcgcca gtacggaaag taagagaaga
     7381 cgaagactga gagcaaagag gtcgccattt cgcagataag aacgcagata gagattttgc
     7441 caaatcttcc caggcacctt ctgaacagcc tttaagataa agtccagata ggcacgacgc
     7501 ttaacgctgt ttgaaattcc cttgacctgc gtaaagaggg caaagaaacg aagcaagact
     7561 tgcttacgct tgctttcttg ggaaatgaca tagtcccaat ccagtccagt ttcagtgaaa
     7621 aatttgctgg ccttttgacg aaagtggaaa tattttccta cccccaataa aagcacatag
     7681 agcagaaaaa ctggcaagcc ataacccatt gctaaaaata aaggcgcaaa taacagcaag
     7741 aaaagggtct gtacaaagag ccaaaagact agggaaatgc cagtttgacg cttgagatgg
     7801 agcttgattt cctcttctcc aactaagaga aagagcttgt ctggagcctc catataggtg
     7861 gcaattcctc cccaaagtaa aagtaaaaca gacgtaattc ctacaaacaa aaggataggc
     7921 caatgatttt caggaaaatg ttgtaagagt tgactgtact ggtaggctag aaagcccaac
     7981 aggacaagca ggaacaagac aaagtggtca ttgagcacat agcgcagata accaagacac
     8041 tccttacgaa aggcctgctt tctctttaaa aacaagtctt tcataggtcc tcctcttttg
     8101 tcagagccaa gtaaatatca ttcaaactag cctcaggcat atcaaaggct tcgcgtagtt
     8161 gcaggagatt gcctttggca cgcacctctc ccttgtgaag aatgacaaag gcatcacaca
     8221 tcttctccgc cgaatccagc acgtgggtac tcatgagaat agacttgccc ttttgcttct
     8281 ccacttccaa aagctgaatc aaatcagaaa tagccagcgg atcaagacca aggaaaggct
     8341 catccacgat gaaaagactt ggatccgcca caaaagcaca gataatcatg accttctgct
     8401 tcatcccttt tgaaaaatga acagggaacc agtctaattt ctgttccaaa cggaacattt
     8461 ttaacaaggg ctctactcgt tcgaaagcca ctttttgctc aataccgtaa gccatagcaa
     8521 ccgtttcgat atgctctctg agagtcaatt cctcatacag actaggcgtc tcaggaatgt
     8581 agccaatctg cttgcggtag ctagtcgcat ctccttgcag agtcaggcca ttgatattga
     8641 tggagccact ataaggtgcc aacagaccga taatctcatt gatcgtcgtt gatttcccag
     8701 caccattgag accaatcaaa ccgaccaact gcccactttc aacagtaaag gacacatctt
     8761 tcaaaacagg aacatgaaca tagccacctg tcaggttttt aatttctaac atattttctc
     8821 caaatctggt ataatgtagc tatattatat caaaattcag tacagtagag gtagatttta
     8881 tgtcagattg cattttttgt aaaatcatcg caggggaaat tcctgcttca aaagtatatg
     8941 aagatgagca ggtccttgcc tttcttgata tctctcaagt aacactagga cacaccttgg
     9001 tcgtgccaaa agaacactat cgcaatcttt tggagatgga tgctacgagc gccagccaac
     9061 tctttgccca agtaccaaaa gtagctcaaa aagtcatgaa agtcactaag gctgctggta
     9121 tgaatatcat ttccaactgt gaagaagtcg ctggtcaaac agtttttcat actcacgttc
     9181 accttgtacc tcgctacagt gctgacgatg acctcaagat tgattttatc gcccacgaac
     9241 cagactttga caaacttgct caagtcgctg aaaccatcaa aaacgcttaa ggagttgcta
     9301 tgaaattatc caacctactg ctatttgcag gagctgcagc cggaagttat ctggtcacaa
     9361 aaaatcgcca aaccatcaca gatgaagtct tgaataccac tgaccgcgtt caagctatca
     9421 aggacgatgt ggatattatc caaaacagcc tgcaaatcat tgaccagcaa aaagaactta
     9481 tcaaggaata ccaagaagac ttgacttaca agtttaaggt cttggaaaag gatatccaga
     9541 ctagactagc tgtgataaaa gaaatgcagg gaactgaaga taagtaaaaa gagcccgacg
     9601 gctctttttt aaaacgtaaa caagttatag tgaattgaat ttagaatagc acatcatagt
     9661 ttctaaagca ttgttagaga ttactttaaa atcctcttat caatttgttc atattctatt
     9721 tcaatctact atatatatcc catcaactat gcatcataat ttaggtaact catactcaat
     9781 aaaaatcaaa aagcaaacta gaaagctagg cacagactgc tcaaaacacc gttttaaggt
     9841 tgtggataga actgacgaag tcagtaacca tacccatgac aagccgacgc tgacgtggtt
     9901 tgaagaaatt tttgaagagt atcattcacc atttcacaac taaatcgaag actttctgcc
     9961 gtaatgaaaa cacctgaaac agcttggttt cggctatccg gaaactttaa gacttaggtt
    10021 caaagtttag gtatggaact tcgaaggagg tcgctaccgt ccgtcattac ttaggaaagt
    10081 cttaaaaaat ctataaagta aaaagagccc gacagctctt tttgtttata ctcaatgaaa
    10141 atcaaaaagc aaactaggaa actagccacg ggctgctcaa aacactgttt tgaggttgta
    10201 gatacgactg acgaagtcag tcacatatat acggcaaggc aacgctgacg tggtttgaag
    10261 agattttcga agagtattat tctccatcaa aggcatcttt aatatggtca aagaagcctt
    10321 ttttctttgg atttactttc aagtcaccag cagccgcgaa ttctttcaag gctacttttt
    10381 ggcggtcgtt caagcctgtc ggtgttacga cattaacagt aacgtattgg tcaccaactg
    10441 caccgccacg aaggctcggt gcccccttac tacgtaggcg gaacttctta ccagtctgag
    10501 ttccctctgg aataaccaat tcaacatcac cgtgaacagt tggaatatct actgtatcac
    10561 caagagccgc ttggacaaag ttgaggttga gattgtagaa gatagtcgtt ccttcacgtt
    10621 caaacttgtc gctagcttcc acagaaacta ctacatacaa gtcaccataa ggtccaccgt
    10681 taaagcctgc ttcaccttga ccagcgaggc gaatttgttg acctgtttcc acaccagcag
    10741 ggattttcac atgtacgcta tgagcttgtt tctcatgacc tgttccatga caggttgtac
    10801 atggatattt gatttctttt cctcgaccgt gacagacatc acaggttact tggcgacgca
    10861 tcataccaag aggagtctgc gtatcgacgt taatgacacc agcgccatga cagcgtccac
    10921 aagtgactgg acttgtccct ggcttagcac cagatccatt acatgtacga cagccagctt
    10981 cacgatgata cttaacttcc ttctcagttc cgaagatagc ttcttcaaag gtcaaattga
    11041 cacgatactg gagatcatct ccttggcgag gagcgtttgg attgcgcgaa gaaccgcctc
    11101 cgccgaagaa acttgagaaa atatcctcaa aaccaccgaa gccacctgcc ccattgaaac
    11161 cgccgaaacc accagctcca ccaaaaccac cattggcgcc tgcagcacca tactggtcat
    11221 aggcagcacg tttttggtcg tcactcaaag tctcataggc ttcttgaact tccttgtact
    11281 tgtcctcagc accaggctcc ttgttgatat ctgggtgata ttttttggaa agcttacgat
    11341 aagccttttt gatttcgtct gccgaagcgt ttttggatac ccccagacga tcataaaatt
    11401 cagtattgtt cataattcaa gatacaaagg gcgtcaaacg gacacagcca tctaggagtt
    11461 ttgacgacgg acgcttacgt cctagaaaaa actatctttt tggcacagtc catagcccgt
    11521 gttcagttac gaacaccctt attcctttct attgatgttc tgaatcgaac caccatttag
    11581 gtcgggttca attcctttct tttatattta gtaagaaatt ctgaaacccg tgttcagtta
    11641 cgaacactct ttgttccttt ctattgatga gacaaaaccc aaacaaatga gattccgtcc
    11701 ctttttatcg aaaaacagag gctgggttgc aacctctgca aataagtaaa ttaactttat
    11761 tttgatttta aatatccta
//
LOCUS       DAVMGZ010000054        11715 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000054 DAVMGZ010000000
VERSION     DAVMGZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.055"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            21..287
                     /locus_tag="VYQ93_001187"
     CDS             21..287
                     /locus_tag="VYQ93_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV3335880.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            280..648
                     /locus_tag="VYQ93_001188"
     CDS             280..648
                     /locus_tag="VYQ93_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV3335881.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            768..2036
                     /locus_tag="VYQ93_001189"
     CDS             768..2036
                     /locus_tag="VYQ93_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV3335882.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFSIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            2033..3310
                     /gene="tilS"
                     /locus_tag="VYQ93_001190"
     CDS             2033..3310
                     /gene="tilS"
                     /locus_tag="VYQ93_001190"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV3335883.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDEFVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            3314..3856
                     /gene="hpt"
                     /locus_tag="VYQ93_001191"
     CDS             3314..3856
                     /gene="hpt"
                     /locus_tag="VYQ93_001191"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV3335884.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            3872..5830
                     /gene="ftsH"
                     /locus_tag="VYQ93_001192"
     CDS             3872..5830
                     /gene="ftsH"
                     /locus_tag="VYQ93_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV3335885.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            5952..6431
                     /locus_tag="VYQ93_001193"
     CDS             5952..6431
                     /locus_tag="VYQ93_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV3335886.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWEQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            6525..6596
                     /locus_tag="VYQ93_001194"
     tRNA            6525..6596
                     /locus_tag="VYQ93_001194"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6558..6560,aa:Glu,seq:ttc)
     gene            6848..8393
                     /locus_tag="VYQ93_001195"
     rRNA            6848..8393
                     /locus_tag="VYQ93_001195"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            8445..8517
                     /locus_tag="VYQ93_001196"
     tRNA            8445..8517
                     /locus_tag="VYQ93_001196"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8478..8480,aa:Ala,seq:tgc)
     gene            8642..11543
                     /locus_tag="VYQ93_001197"
     rRNA            8642..11543
                     /locus_tag="VYQ93_001197"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            11620..>11715
                     /gene="rrf"
                     /locus_tag="VYQ93_001198"
     rRNA            11620..>11715
                     /gene="rrf"
                     /locus_tag="VYQ93_001198"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3769 a         2010 c         2872 g         3064 t
ORIGIN      
        1 aataaattga ggtaataagg atgagattag ataaatattt aaaagtatcg cgaattatca
       61 agcgtcgtac agtcgcaaag gaagtagcag ataaaggtag aatcaaggtt aatggaatct
      121 tggccaaaag ttcaacggac ttgaaagtta atgaccaagt tgaaattcgc tttggcaata
      181 agttgctgct tgtaaaagta ctagagatga aagatagtac aaaaaaagaa gatgcagcag
      241 gaatgtatga aattatcagt gaaacacggg tagaagaaaa tgtctaaaaa tattgtacaa
      301 ttgaataatt cttttattca aaatgaatac caacgtcgtc gctacctgat gaaagaacga
      361 caaaaacgga atcgttttat gggaggggta ttgattttga ttatgctatt atttatcttg
      421 ccaactttta atttagcgca gagttatcag caattactcc aaagacgtca gcaattagca
      481 gacttgcaaa ctcagtatca aactttgagt gatgaaaagg ataaggagac agcatttgct
      541 accaagttga aagatgaaga ttatgctgct aaatatacac gagcgaagta ctattattct
      601 aagtcgaggg aaaaagttta tacgattcct gacttgcttc aaaggtgata aaatggaaaa
      661 tttattagac gtaatagagc aatttttgag tttgtcagat gaaaagctgg aagaattggc
      721 tgataaaaat caattattgc gtttacaaga agaaaaggaa aggaagaatg cgtaaattct
      781 taattatttt gttgctacca agttttttga ccatttcaaa agtcgttagc acagaaaaag
      841 aagtcgtcta tacttcgaaa gaaatttatt acctttcaca atctgacttt agtatttatt
      901 ttagagaaaa attaagttct cccatggttt atggagaggt tcctgtttat gcgaatgaag
      961 atttagtagt ggaatctggg aaattgactc ccaaaacaag ttttcaaata accgagtggc
     1021 gcttaaataa acaaggaatt ccagtattta agctatcaaa tcatcaattt atagctgcgg
     1081 acaaacgatt tttatatgat caatcagagg taactccaac aataaaaaaa gcatggttag
     1141 aatctgactt taaactgtac aatagtcctt atgatttaaa agaagtgaaa tcatccttat
     1201 cagcttattc gcaagtatca atcgacaaga ccatgtttgt agaaggaaga gaatttctac
     1261 atattgatca ggctggatgg gtagctaaag aatcaacttc tgaagaagat aatcggatga
     1321 gtaaagttca agaaatgtta tctgaaaaat atcagaaaga ttctttctct atttatgtta
     1381 agcaactgac tactggaaaa gaagctggta tcaatcaaga tgaaaagatg tatgcagcca
     1441 gcgttttgaa actctcctat ctctattata cgcaagaaaa aataaatgag ggtctttatc
     1501 agttagatac gactgtaaaa tacgtatctg cagtcaatga ttttccaggt tcttataaac
     1561 cagagggaag tggtagtctt cctaaaaaag aagataataa agaatattct ttaaaggatt
     1621 taattacgaa agtatcaaaa gaatctgata atgtagctca taatctattg ggatattaca
     1681 tttcaaacca atctgatgcc acattcaaat ccaagatgtc tgccattatg ggagatgatt
     1741 gggatccaaa agaaaaattg atttcttcta agatggccgg gaagtttatg gaagctattt
     1801 ataatcaaaa tggatttgtg ctagagtctt tgactaaaac agattttgat agtcagcgaa
     1861 ttgccaaagg tgtttctgtt aaagtagctc ataaaattgg agatgcggat gaatttaagc
     1921 atgatacggg tgttgtctat gcagattctc catttattct ttctattttc actaagaatt
     1981 ctgattatga tacgatttct aagatagcca aggatgttta tgaggttcta aaatgaggga
     2041 accagatttt ttaaatcatt ttctcaagaa gggatatttc aaaaagcatg ctaaggcggt
     2101 tctagctctt tctggtggat tagattccat gtttctattt aaggtattgt ctacttatca
     2161 aaaagagtta gagattgaat tgattctagc tcatgtgaat cataagcaga gaattgaatc
     2221 agattgggaa gaaaaggaat taaggaagtt ggctgctgaa gcagagcttc ctatttatat
     2281 cagcaatttt tcaggagaat tttcagaagc gcgtgcacga aattttcgtt atgatttttt
     2341 tcaagaggtc atgaaaaaga caggtgcgac agctttagtc actgcccacc atgctgatga
     2401 tcaggtggaa acgattttga tgcgcttgat tcgaggaact cgcttgcgct atctatcagg
     2461 aattaaggag aagcaagtag tcggagagat agaaatcatt cgtcccttct tgcattttca
     2521 gaaaaaagac tttccatcaa tttttcactt tgaagataca tcaaatcagg agaatcatta
     2581 ttttcgaaat cgtattcgaa attcttactt accagaattg gaaaaagaaa atcctcgatt
     2641 tagggatgca atcttaggca tcggcaatga aattttagat tatgatttgg caatagctga
     2701 attatctaac aatattaatg tggaaaattt acagcagtta ttttcttact ctgagtctac
     2761 acaaagagtt ttacttcaaa cttatctgaa tcgttttcca gatttgaatc ttacaaaagc
     2821 tcagtttgct gaagttcagc agattttaaa atctaaaagc cagtatcgtc atccgattaa
     2881 aaatggctat gaattgataa aagagtacca acagtttcag atttgtaaaa tcagtccgca
     2941 ggctgatgaa gaggaagatg aatttgtgtt acactatcaa aatcaggtag cttatcaagg
     3001 atatttattt tcctttggac ttccattaga aggtgaatta attcaacaaa tacctgtttc
     3061 acgtgaaaca tccatacaca ttcgtcatcg aaaaacagga gatgttttga ttcaaaatgg
     3121 gcatagaaaa aaactcagac gtttatttat tgatttgaaa atccctatgg aaaagagaaa
     3181 ctctgctctt attattgagc aatttggtga aattgtctca attttgggaa ttgcgaccaa
     3241 taatttgagt aaaaaaacga aaaatgatat aatgaacact gtactttata tagaaaaaat
     3301 agataggtaa aaaatgttag aaaacgatat taaaaaagtc ctcgtttcac acgatgaaat
     3361 tacagaagca gctaaaaaac taggtgctca attaactaaa gactatgcag gaaaaaatcc
     3421 aatcttagtt gggattttaa aaggatctat tccttttatg gctgaattgg tcaaacatat
     3481 tgatacacat attgaaatgg acttcatgat ggtttctagc taccatggtg gaacagcaag
     3541 tagtggtgtt atcaatatta aacaagatgt gactcaagat atcaaaggaa gacatgttct
     3601 atttgtagaa gatatcattg atacaggtca aactttgaag aatttgcgag atatgtttaa
     3661 agaaagagaa gcagcttctg ttaaaattgc aaccttgttg gataaaccag aaggacgtgt
     3721 tgtagaaatt gaggcagact atacctgctt tactatccca aatgagtttg tagtaggtta
     3781 tggtttagac tacaaagaaa attatcgtaa tcttccttat attggagtat tgaaagagga
     3841 agtgtattca aattagaaag aataatcttt aatgaaaaaa caaaataatg gtttaattaa
     3901 aaatcctttt ctatggttat tatttatctt tttccttgtg acaggattcc agtatttcta
     3961 ttctgggaat aactcaggag gaagtcagca aatcaactat actgagttgg tacaagaaat
     4021 taccgatggt aatgtaaaag aattaactta ccaaccaaat ggtagtgtta tcgaagtttc
     4081 tggtgtctat aaaaatccta aaacaagtaa agaaggaaca ggtattcagt ttttcacgcc
     4141 atctgttact aaggtagaga aatttaccag cactattctt cctgcagata ctaccgtatc
     4201 agaattgcaa aaacttgcta ctgaccataa agcagaagta actgttaagc atgaaagttc
     4261 aagtggtata tggattaatc tactcgtatc cattgtgcca tttggaattc tattcttctt
     4321 cctattctct atgatgggaa atatgggagg aggcaatggc cgtaatccaa tgagttttgg
     4381 acgtagtaag gctaaagcag caaataaaga agatattaaa gtaagatttt cagatgttgc
     4441 tggagctgaa gaagaaaaac aagaactagt tgaagttgtt gagttcttaa aagatccaaa
     4501 acgattcaca aaacttggag cccgtattcc agcaggtgtt cttttggagg gacctccggg
     4561 gacaggtaaa actttgcttg ctaaggcagt cgctggagaa gcaggtgttc cattctttag
     4621 tatctcaggt tctgactttg tagaaatgtt tgtcggagtt ggagctagtc gtgttcgctc
     4681 tctttttgag gatgccaaaa aagcagcacc agctatcatc tttatcgatg aaattgatgc
     4741 tgttggacgt caacgtggag tcggtctcgg cggaggtaat gacgaacgtg aacaaacctt
     4801 gaaccaactt ttgattgaga tggatggttt tgagggaaat gaagggatta tcgtcatcgc
     4861 tgcgacaaac cgttcagatg tacttgatcc tgcccttttg cgtccaggac gttttgatag
     4921 aaaagtattg gttggccgtc ctgatgttaa aggtcgtgaa gcaatcttga aagttcacgc
     4981 taagaacaag cctttagcag aagatgttga tttgaaatta gtggctcaac aaactccagg
     5041 ctttgttggt gctgatttag agaatgtctt gaatgaagca gctttagttg ctgctcgtcg
     5101 caataaatcg ataattgatg cttcagatat tgatgaagca gaagatagag ttattgctgg
     5161 accttctaag aaagataaga cagtttcaca aaaagaacga gaattggttg cttaccatga
     5221 ggcaggacat accattgttg gtctagtctt gtcgaatgct cgcgttgtcc ataaggttac
     5281 aattgtacca cgcggccgtg caggcggata catgattgca cttcctaaag aggatcaaat
     5341 gcttctatct aaagaagata tgaaagagca attggctggc ttaatgggtg gacgtgtagc
     5401 tgaagaaatt atctttaatg tccaaactac aggagcttca aacgactttg aacaagcgac
     5461 acaaatggca cgtgcaatgg ttacagagta cggtatgagt gaaaaacttg gcccagtaca
     5521 atatgaagga aaccatgcta tgcttggtgc acagagtcct caaaaatcaa tttcagaaca
     5581 aacagcttat gaaattgatg aagaggttcg ttcattatta aatgaggcac gaaataaagc
     5641 tgctgaaatt attcagtcaa atcgtgaaac tcacaagtta attgcagaag cattattgaa
     5701 atacgaaaca ttggatagta cacaaattaa agctctttac gaaacaggaa agatgcctga
     5761 agcagtagaa gaggaatctc atgcactatc ctatgatgaa gtaaagtcaa aaatgaatga
     5821 cgaaaaataa ccctgagaga ggctggagcc tctctttttt gtgcagttta ggagctaaag
     5881 ggaacagaat ggagaaaatg gaacaaatgt gttttctaat ctgttagact gtatctagaa
     5941 aggggaaaat tatgattaaa gaattgtatg aagaagtcca agggactgtg tataagtgta
     6001 gaaatgaata ttaccttcat ttatgggaat tgtcggattg ggagcaagaa ggcatgctct
     6061 gcttacatga attgattagt agagaagaag gactggtaga cgatattcca cgtttaagga
     6121 aatatttcaa gaccaagttt cgaaatcgaa ttttagacta tatccgtaaa caggaaagtc
     6181 agaagcgtag atacgataaa gaaccctatg aagaagtggg tgagatcagt catcgtataa
     6241 gtgagggggg tctctggcta gatgattatt atctctttca tgaaacacta agagattata
     6301 gaaacaaaca aagtaaagag aaacaagaag aactagaacg cgtcttaagc aatgaacgat
     6361 ttcgagggcg tcaaagagta ttaagagact tacgcattgt gtttaaggag tttactatcc
     6421 gtacccatta gtaagtcatg caaaaaaaat gaaaaaaatt agaaaaagta gttgacaaag
     6481 tttgaaaaga ctgtataata gtaagagttg aaaataacaa ctcaggtccg ttggtcaagg
     6541 ggttaagaca ccgccttttc acggcggtaa cacgggttcg aatcccgtac ggactatggt
     6601 atgttgcgtc aggaccactt gatgaaaaaa agtttaaaaa agcttaaaaa tcttcaaaaa
     6661 agtgttgaca agtgaaagtg gctgtgatat actaatatag ttgtcgcttg agagaagcaa
     6721 gtgacaaaga cctttgaaaa ctgaacaaga cgaaccaatg tgcagggcgc tacaacataa
     6781 gttgtagtac tgaacaatga aaaaacaata aatctgtcag tgacagaaat gagtaagaac
     6841 tcaaactttt aatgagagtt tgatcctggc tcaggacgaa cgctggcggc gtgcctaata
     6901 catgcaagta gaacgctgaa ggaggagctt gcttctctgg atgagttgcg aacgggtgag
     6961 taacgcgtag gtaacctgcc tggtagcggg ggataactat tggaaacgat agctaatacc
     7021 gcataagagt agatgttgca tgacatttgc ttaaaaggtg cacttgcatc actaccagat
     7081 ggacctgcgt tgtattagct agttggtggg gtaagggctc accaaggcga cgatacatag
     7141 ccgacctgag agggtgatcg gccacactgg gactgagaca cggcccagac tcctacggga
     7201 ggcagcagta gggaatcttc ggcaatggac ggaagtctga ccgagcaacg ccgcgtgagt
     7261 gaagaaggtt ttcggatcgt aaagctctgt tgtaagagaa gaacgagtgt gagagtggaa
     7321 agttcacact gtgacggtat cttaccagaa agggacggct aactacgtgc cagcagccgc
     7381 ggtaatacgt aggtcccgag cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg
     7441 ttagataagt ctgaagttaa aggctgtggc ttaaccatag taggctttgg aaactgttta
     7501 acttgagtgc aagaggggag agtggaattc catgtgtagc ggtgaaatgc gtagatatat
     7561 ggaggaacac cggtggcgaa agcggctctc tggcttgtaa ctgacgctga ggctcgaaag
     7621 cgtggggagc aaacaggatt agataccctg gtagtccacg ctgtaaacga tgagtgctag
     7681 gtgttagacc ctttccgggg tttagtgccg tagctaacgc attaagcact ccgcctgggg
     7741 agtacgaccg caaggttgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc
     7801 atgtggttta attcgaagca acgcgaagaa ccttaccagg tcttgacatc cctctgaccg
     7861 ctctagagat agagctttcc ttcgggacag aggtgacagg tggtgcatgg ttgtcgtcag
     7921 ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccctat tgttagttgc
     7981 catcattcag ttgggcactc tagcgagact gccggtaata aaccggagga aggtggggat
     8041 gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa tggctggtac
     8101 aacgagtcgc aagccggtga cggcaagcta atctcttaaa gccagtctca gttcggattg
     8161 taggctgcaa ctcgcctaca tgaagtcgga atcgctagta atcgcggatc agcacgccgc
     8221 ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac
     8281 ccgaagtcgg tgaggtaacc gtaaggagcc agccgcctaa ggtgggatag atgattgggg
     8341 tgaagtcgta acaaggtagc cgtatcggaa ggtgcggctg gatcacctcc tttctaagga
     8401 taaggaactg cgcattggtc ttgtttagtc ttgagaggtc ttgtggggcc ttagctcagc
     8461 tgggagagcg cctgctttgc acgcaggagg tcagcggttc gatcccgcta ggctccattg
     8521 gtgagagatc accaagtaat gcacattgaa aattgaatat ctatatcaaa tagtaacaag
     8581 aaaataaacc gaaaacgctg tagtattaat aagagtttat gactgaaagg tcagaaaata
     8641 aggttaagtt aataagggcg cacggtggat gccttggcac taggagccga cgaaggacgt
     8701 gacaaacgac gatatgcctt gggtagctgt aagtaagcga tgatccaggg atttccgaat
     8761 gggggaaccc aacaggtaat acctgttacc tacatctgtt aaggatgtga ggaggaagac
     8821 gcagtgaact gaaacatcta agtagctgca ggaagagaaa gcaaaagcga ttgccttagt
     8881 agcggcgagc gaaacggcag aagggcaaac cgaagagttt actcttcggg gttgtaggac
     8941 tgcaatgtgg actcaaagat tatagaagaa tgatttggga agatcagcca aagagagtaa
     9001 tagcctcgta tttaaaatag tctttgtact tagcagtatc ctgagtacgg cgggacacgt
     9061 gaaatcccgt cggaatctgg gaggaccatc tcccaaccct aaatactccc tagtgaccga
     9121 tagtgaacca gtaccgtgag ggaaaggtga aaagcacccc gggaggggag tgaaatagaa
     9181 cctgaaaccg tgtgcctaca acaagttcga gcccgttaat gggtgagagc gtgccttttg
     9241 tagaatgaac cggcgagtta cgttatgatg cgaggttaag ttgaagagac ggagccgtag
     9301 ggaaaccgag tctgaatagg gcgccttagt atcatgacgt agacccgaaa ccatgtgacc
     9361 tacccatgag caggttgaag gtgcggtaag acgcactgga ggaccgaacc agggcacgtt
     9421 gaaaagtgct tggatgactt gtgggtagcg gagaaattcc aaacgaactt ggagatagct
     9481 ggttctctcc gaaatagctt tagggctagc gtcgacatta gagattcttg gaggtagagc
     9541 actgtttggg tgaggggtcc atcccggatt accaatctca gataaactcc gaatgccaat
     9601 gaattatggt cggcagtcag actgcgagtg ctaagatccg tagtcgaaag ggaaacagcc
     9661 cagaccacca gctaaggtcc caaaataatt gttaagtgga aaaggatgtg gggttgcaca
     9721 gacaactagg atgttagctt agaagcagct attcattcaa agagtgcgta atagctcact
     9781 agtcgagtga ccctgcgccg aaaatgtacc ggggctaaaa caatttaccg aagctgtgga
     9841 tacctttata ggtatggtag gagagcgttc tatgtgtgat gaaggtatac cgtgaggagt
     9901 gctggaacgc atagaagtga gaatgccggt atgagtagcg aaagacaggt gagaatcctg
     9961 tccaccgtaa gactaaggtt tccaggggaa ggctcgtccg ccctgggtta gtcgggacct
    10021 aaggagagac cgaaaggtgt atccgatgga caacaggttg atattcctgt actagagtat
    10081 gtagtgatgg agggacgcag taggctaact aaagcagacg attggaagag tctgtctaag
    10141 cagtgaggtg tgaattgagt caaatgctta attctataac attgagctgt gatggggagc
    10201 gaagtttagt agcgaagtta gtgacgtcac actgccaaga aaagcttcta gcgtttaaac
    10261 atactctacc cgtaccgcaa accgacacag gtagtcgagg cgagtagcct caggtgagcg
    10321 agagaactct cgttaaggaa ctcggcaaaa tgaccccgta acttcgggag aaggggtgct
    10381 gacttaaagt cagccgcagt gaataggccc aagcaactgt ttatcaaaaa cacagctctc
    10441 tgctaaatcg taagatgatg tatagggggt gacgcctgcc cggtgctgga aggttaagag
    10501 gagtgcttag cgtaagcgaa ggtatgaatt gaagccccag taaacggcgg ccgtaactat
    10561 aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacccgcac gaaaggcgta
    10621 atgatttggg cactgtctca acgagagact cggtgaaatt ttagtacctg tgaagatgca
    10681 ggttacccgc gacaggacgg aaagacccca tggagcttta ctgcagtttg atattgagtg
    10741 tctgtaccac atgtacagga taggtaggag tctaagagat cgggacgcca gtttcgaagg
    10801 agacgctgtt gggatactac ccttgtgtta tggccactct aacccagata ggtgatccct
    10861 atcggagaca gtgtctgacg ggcagtttga ctggggcggt cgcctcctaa aaggtaacgg
    10921 aggcgcccaa aggttccctc agaatggttg gaaatcattc gcagagtgta aaggtataag
    10981 ggagcttgac tgcgagagct acaactcgag cagggacgaa agtcgggctt agtgatccgg
    11041 tggttccgta tggaagggcc atcgctcaac ggataaaagc taccctgggg ataacaggct
    11101 tatctccccc aagagttcac atcgacgggg aggtttggca cctcgatgtc ggctcgtcgc
    11161 atcctggggc tgtagtcggt cccaagggtt gggctgttcg cccattaaag cggcacgcga
    11221 gctgggttca gaacgtcgtg agacagttcg gtccctatcc gtcgcgggcg taggaaattt
    11281 gagaggatct gctcctagta cgagaggacc agagtggact taccgctggt gtaccagttg
    11341 tcttgccaaa ggcatcgctg ggtagctatg tagggaaggg ataaacgctg aaagcatcta
    11401 agtgtgaaac ccacctcaag atgagatttc ccatgattat atatcagtaa gagccctgag
    11461 agatgatcag gtagataggt tagaagtgga agtgtggcga cacatgtagc ggactaatac
    11521 taatagctcg aggacttatc caaagtaact gagaatatga aagcgaacgg ttttcttaag
    11581 ttgaatagat attcaatttt gagtaggtat tactcagagt taagtgacga tagcctagga
    11641 gatacacctg tacccatgcc gaacacagaa gttaagccct agaacgccgg aagtagttgg
    11701 gggttgcccc ctgtg
//
LOCUS       DAVMGZ010000055        11674 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000055 DAVMGZ010000000
VERSION     DAVMGZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11674
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.056"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            96..749
                     /locus_tag="VYQ93_001199"
     CDS             96..749
                     /locus_tag="VYQ93_001199"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV3335887.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            759..1223
                     /locus_tag="VYQ93_001200"
     CDS             759..1223
                     /locus_tag="VYQ93_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV3335888.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1236..2504
                     /locus_tag="VYQ93_001201"
     CDS             1236..2504
                     /locus_tag="VYQ93_001201"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV3335889.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     NATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2518..2778
                     /locus_tag="VYQ93_001202"
     CDS             2518..2778
                     /locus_tag="VYQ93_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335890.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2789..4150
                     /gene="pdrM"
                     /locus_tag="VYQ93_001203"
     CDS             2789..4150
                     /gene="pdrM"
                     /locus_tag="VYQ93_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV3335891.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAVFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4304..5272
                     /locus_tag="VYQ93_001204"
     CDS             4304..5272
                     /locus_tag="VYQ93_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV3335892.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGSVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5288..6280
                     /locus_tag="VYQ93_001205"
     CDS             5288..6280
                     /locus_tag="VYQ93_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV3335893.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEEISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCSGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            6498..7541
                     /locus_tag="VYQ93_001206"
     CDS             6498..7541
                     /locus_tag="VYQ93_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV3335894.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7605..9290
                     /gene="lpdA"
                     /locus_tag="VYQ93_001207"
     CDS             7605..9290
                     /gene="lpdA"
                     /locus_tag="VYQ93_001207"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV3335895.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9354..10343
                     /locus_tag="VYQ93_001208"
     CDS             9354..10343
                     /locus_tag="VYQ93_001208"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV3335896.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10416..11486)
                     /gene="xerS"
                     /locus_tag="VYQ93_001209"
     CDS             complement(10416..11486)
                     /gene="xerS"
                     /locus_tag="VYQ93_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV3335897.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAKNIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3454 a         2193 c         2631 g         3396 t
ORIGIN      
        1 tagtcgattt attaaatctt tagataaact gtaaaaagtg gtttccatag ccactttttg
       61 ctataataga ggcagtaaaa atgaaggagt cagctatgga acactcgtct tggcatgctt
      121 tgattaaggc gcaattacct gagggttatt tcgggaaaat caatcagttt atggagcagg
      181 tctattctca ggggattatt tatccaccca aggaaaaggt ttttcaggct ctcttgacaa
      241 cactgcttga agaagttaag gtggtaattc tagggcaaga cccctatcac ggaccaggtc
      301 aagcgcaggg cttgagtttt tctgtacctg actctatccc agctccacca tccttgcaaa
      361 atatcttgaa agaattgtca gatgatatcg gagttaagaa atctcatgat ttgacagctt
      421 gggctgagca aggagtcttg cttcttaatg cttgtttgac tgttcctgct ggacaggcca
      481 atggtcatgc tggtcaaatc tgggagcctt ttactgatgc tgtgattcag gtggtcaatc
      541 atctagatag accagtcgtt tttgtactct ggggagctta tgcacgtaag aagaaggcct
      601 tagttaccaa tcctcatcac ttgattattg aatcagccca tccaagtcct ttgtcggttt
      661 atagaggatt ttggggttcc aagccctttt ccaaggccaa tgcattctta aaagagacag
      721 gacaagagcc aatcgattgg cttagataag gagaaaatat gcctcagtta gcgacgattt
      781 gctacattga taatgggaaa gaactgctca tgctccatcg taataagaaa cccaatgatg
      841 tccatgaagg gaaatggatt ggtgtgggtg gtaagctaga gagaggagag acgccccagg
      901 aatgcgcggc gcgtgaaatc cttgaagaaa cagggctcaa agccaagcca gttctaaaag
      961 gtgtcatcac ttttcctgaa tttacaccag atttagactg gtacacctat gtttttaagg
     1021 tgacggagtt tgagggcgac ttgattgact gcaatgaggg gacgctagaa tgggttccct
     1081 atgacgaggt tttgagcaag ccgacttggg aaggtgacca cacctttgtt gagtggcttt
     1141 tagaggataa acccttcttt tcagccaagt ttgtttatga tggggataaa ttgttggata
     1201 cccaagttga tttctatgaa taaaggagaa agcagatgct actaatcaaa aatggtcgtg
     1261 taatggatcc taagtctggt ttggatcaag tttgtgatgt cttagttcaa gatgggaaaa
     1321 ttatcaaaat tgcgcctgag atcacggaag aaggagcaga aacgattaat gctactggtc
     1381 ttgtggttgc tcctggtttg gtcgatattc atgtgcattt ccgtgaacct ggtcaaacac
     1441 ataaagaaga cattcatact ggtgccctag cagccgctgc aggtggtttt actactgtcg
     1501 tcatgatggc taataccagt ccaaccattt cagacgtgga gactttgcaa gcagttctcc
     1561 agtcagctgc caaagagaag attaatgtca agacagttgc gaccattact aaaaacttta
     1621 atggtaaaaa cttgactgac tttaaggcgc tcttagaagc tggtgcggtt ggtttctctg
     1681 atgacggtat tccgcttgag agcagtaaga ttgtcaagga agccatggag gaagccaaaa
     1741 aactcaatac ctttatcagc cttcatgagg aagatccagg tttgaacggt gttcttggct
     1801 ttaatgaaaa tattgctaga gaacattttc atatctgcgg tgctactggg gtggctgagt
     1861 acgctatgat ggcgcgtgat gtcatgattg cctatgcaac taaagcccat gttcacatcc
     1921 agcatttgtc taaggaagaa agtgttaaag tagtggagtt tgctcagggg ttaggtgcag
     1981 aagtcacagc agaagtagcg ccacagcatt tctctaagac agaagcactt cttttaacac
     2041 aaggtagcaa tgctaagatg aatccaccgc ttcgtttgga atcagaccgt cgtgccgtta
     2101 tcgaaggtct caaatcaggt gtcatcacag ttattgcgac tgaccacgcg cctcatcatg
     2161 tagatgaaaa aaatgttgag gatattacca aagcgccatc tggtatgact ggcttagaaa
     2221 catccctgtc tctcagcttg acctatttag tagaagctgg tgagttgagc ttgatggaat
     2281 tacttgaaaa aatgacatac aacccagcca agctttacaa ctttgaagca ggttacttgg
     2341 ctgagaatgg tccagcagat atcactattt ttgatgccaa ggctgaccgc cttgtggact
     2401 cccattttgc ttccaaagca gctaattcac cattcatcgg tgaaacctta aaagggcagg
     2461 ttaaatatac catctgtaag ggacaaatcg tctatcaagc ttgataggag ttagaatatg
     2521 aattattttc gaaaacgtag ggagagacaa gcgaaatcta atagtggaat ttactgtccc
     2581 gcagctaatc gttccaaaat tcaatatgaa actaaagaaa aagctgacca tgcggttcaa
     2641 tatgataacg gaggtttagt tagaagctat tattgtcgta catgtgcgtg ttggcatact
     2701 acttcaaaat ctaataaacc tccgctaagt ttgaaaaact atggtctgaa actctttggt
     2761 ttagataaat cagaatagaa agagaagtat gtataagaca aagtgtttac gagagaagtt
     2821 agtattattt ttaaaaattt tcttcccaat actgatctac caatttgcca attattctgc
     2881 ctcttttgtt gatactgcaa tgacaggtca atacaacact atggacttgg ctggtgtatc
     2941 tatggcaacc agtatctgga atcctttctt tacatttcta acagggattg tgtcagcctt
     3001 ggtgcctatc attggtcacc atcttggtcg aggaaaaaag gaagaagttg cgtctgattt
     3061 ttaccaattt atttatttgg ccttgggcct atctgtggtc ttgctgggga tggtactttt
     3121 cttggcacca acaatcttga atcatattgg gttagaagca gcagtagcgg cagtagcggt
     3181 tcgctatctt tggtttttat ctatcgggat tatccccttg ttgctcttta gcgtcattcg
     3241 ttccttgctg gattcgctgg gcttgaccaa actgtccatg tacctcatgc ttttgttact
     3301 ccctctcaat agcggattta actatctctt gatttacggt gcctttggtg ttccagaact
     3361 gggaggggct ggtgctggtt taggaacatc cttggcctac tgggtcttgc ttgggatttc
     3421 tgttctggtt ttatttaaac aggagaagct caaagcctta caccttgaga aacgaattcc
     3481 acttaatatg gataaaatta aggaaggagt tcgtttaggt ctgcctattg ggggaactgt
     3541 cttcgcggaa gtggctgtct tttcagtggt tggcttgatt atggctaagt tttcgtcctt
     3601 gattatagct agtcaccagt cagctatgaa cttttcaagt cttatgtacg cctttcctat
     3661 gagtatctca tcggctatgg ctattgtcgt ttcctatgaa gtgggagcca agcgatttga
     3721 tgatgcgaaa acctatattg gtctaggaag atggactgcc ctcatttttg cggccttcac
     3781 cttaaccttc ctttacattt ttaggggaaa tgtggccagt ctttatggta acgacccaaa
     3841 atttatcgat ttgacagcgc gttttttaac ttatagtctt ttcttccagt tagcagatac
     3901 ctttgcggcg ccgcttcagg gaattttgcg ggggtataag gatacagtta ttccttttta
     3961 ccttggtttg cttggttatt ggggcgtagc aatccctgtg gctacgctat ttgattccct
     4021 aacagatttt ggagcttatt cttactggat cggcttgatt attagtttga ttgtgagcgg
     4081 ggcgctctac cgttggcgtt taactgtgat tatgaagaga tttgaatctt tagcaaaatc
     4141 caaacgctaa aaagtttgtg aaaaaatatt cttgacaaga aaatcccttg ttttcacggg
     4201 gttttctttt ttattttgtg aaatttcttt tagaaaaact ttgacagtat ttgccaaaag
     4261 cggtaaaata gtaaggtaag aagaaagtta gaaaggcaag aagatgtcaa ctttagataa
     4321 aaatcttttg ctagaaatgt tccgtaagat ggaagaaatc cgtcgcatgg acttaaaaat
     4381 tgcacaatta gtaaagaaag ggaaagtgcc aggaatgacg cacttttctg ttggtgaaga
     4441 ggcagctaac gtgggggcta tgttagctct caatccagat gatctgatta cctcaaacca
     4501 ccgtggacac gggcaagcta ttgctaaagg gattgacctc aacggaatga tggctgaaat
     4561 ccttggtaaa tacactggaa cctgtaaagg gaaaggtgga tctatgcata tcgctgacct
     4621 tgatgctggg aaccttggtg ccaatggtat cgtaggtggt ggtatgggga tcgctgtcgg
     4681 tgcagccctc agtcagcaaa tgcaaaatac cggtaaaatc gttgtttgct tctttggaga
     4741 tggtgcgacc aatgaaggtg ttttccacga agcagtgaac atggcttcta tctggaacct
     4801 gccagtcatt ttctattgca ttaacaacgg ttacggtatc tctgcggata tcaagaaaat
     4861 gaccaatata gaacatatcc atcaacgtag cgccgcttat ggaattcctg gaatgttcat
     4921 cgaagacggt aacaatgtca tcgatgtcta tgaaggattt cagaaagctg tagaccatgt
     4981 tcgcagtggc aatggttcag tcttgattga aagtgtaact tatcgctggc tcggtcactc
     5041 atcatctgac cctggtaaat atcgtacgcg tgaagaagtg gaattgtgga aacaaaaaga
     5101 tccaatcgaa aacctccgca attaccttat tgaaaataac attgcaagtg ccgaagaatt
     5161 ggaagaaatc caagcgcaag taaaggaagc agtagaagct tctgttaaat ttgcagagga
     5221 aagcccattc ccaccgcttg aatcagcctt tgaagatatt tacgcagact aaggagaaag
     5281 agataaaatg gaaacaaaaa caatgtcctt ccgtgacacc attatccttg ctatgtctga
     5341 ggaaatgcgt cgcgatgaaa atgtgttctt gatgggagaa gatgtcggtg tcttcggagg
     5401 agacttcggt acttctgttg gaatgcttga agaatttggt ccagaacgtg tccgtgactg
     5461 tccgatttct gaagctgcca tctctggagc agcagcagga gcagccatga caggacttcg
     5521 tccaatcgtc gatatgacct tcatggactt ctctgttatt gccatggaca atatcgtcaa
     5581 ccaagctgct aaaacacgtt acatgtttgg tggtaaaggt caggttccaa tgactgttcg
     5641 ttgtgcagct ggtaacggag ttggctctgc cgcccagcac tcacaatctc tagagtcttg
     5701 gtttactcac attccaggac ttaaggttgt ggcacctgga acacctgcgg acatgaaagg
     5761 cctgcttaag tcttctatcc gtgataacaa cccagttatt atacttgagt acaagtcaga
     5821 atttaaccaa aaaggggaag tgccagttga tccagactac acaattccac ttggggttgg
     5881 tgaaatcaaa cgccaaggaa cggatgtaac agttgttact tatggaaaaa tgcttcgccg
     5941 tgtggttcaa gctgctgaag aattagcaga agaagaaatt tcagttgaaa ttgttgaccc
     6001 acgtaccctt gttccgcttg ataaggatat catcataaac tcagtgaaga agactggtaa
     6061 ggttattctg gtcaacgatg cccacaaaac aagtggctat atcggtgaaa tttcagctat
     6121 tatttcagaa tcagaagcat ttgactatct agatgcacca atacgccgtt gttcaggaga
     6181 agatgtgccg atgccttatg cacaaaacct agaaaatgca atgattccaa cagttgaaag
     6241 tatcaaagat gcaatccgaa aaacttataa caaagactag agttacataa tataagttat
     6301 gtaactaaaa taaagctgac gagaaacttt gtatctttta ggtgcagagc tcttcgtcat
     6361 taatatctta tattcgagca cgggctaaaa gctcggaaaa aagataaatc tccttggctt
     6421 catcgaagcc atcgtcgatt tcctattttt cagtcgcttt tgacgccctt tgtatcataa
     6481 atagaattgg agaggtcatg gctgatgaca agctaagagc gactcctgca gctagaaagt
     6541 tagcggatga tttagggata aacctctacg acgtttctgg ctcaggtgca aacggtcgtg
     6601 tccacaaaga agacgtggaa acttataaag acacaaacgt ggttcgcatt tcgccacttg
     6661 caaaacgaat tgccctcgaa cataacattg cttggcagga aatccaagga accggtcatc
     6721 gtggtaaaat catgaagaag gatgttttgg ccctgcttcc tgaaaatatc gaaaacgata
     6781 gcatcaaatc tcctgctcag attgaaaaag tggaagaagt ccctgataac gtaacaccat
     6841 atggtgaaat cgagcgtatt ccaatgacac caatgcgtaa ggttattgcc caacgcatgg
     6901 ttgaatctta cttgactgcg ccaaccttca ccctcaacta tgaagttgat atgactgaaa
     6961 tgttggctct tcgtaagaag gttcttgagc caatcatgga agcaactggg aagaagacta
     7021 ctgtaacaga ccttctttcg cttgcagttg ttaagactct tatgaaacac ccatacatca
     7081 acgcttcatt gacagaagat ggcaagacta ttatcactca caactatgtc aatcttgcca
     7141 tggcagttgg gatggataat ggattgatga cacctgttgt ttacaatgct gagaagatga
     7201 gtctttcaga actggtggta gcctttaagg atgttattgg ccgtaccttg gatggtaaat
     7261 tggctccaag tgagttacaa aattcaacat tcacaatcag taatttggga atgtttggtg
     7321 ttcagtcctt tggtccgatt attaaccaac ccaactcagc tatccttggt gtcagttcga
     7381 caatcgagaa gccagttgtc gtcaatggtg aaattgtgat tcgcccaatc atgagtttag
     7441 gattaaccat tgaccaccgt gtcgtagatg gtatggctgg tgctaagttt atgaaggact
     7501 tgaaagagtt gattgaaacg ccaatctcaa tgttaattta agagcaagag tattcatttt
     7561 taagatatag ataaaggaag gaagacatgg ccttagaaat aattatgcca aaagccggcg
     7621 tggatatgac agaaggacaa atcgtccaat ggaataaaaa agtcggagaa tttgtaaaag
     7681 aaggagaaat ccttttggaa atcatgactg ataaagtcag catggaattg gaagccgaag
     7741 aagatgggta cttgattgcc attctcaaag gagatggtga aactgtccct gtaacggaag
     7801 ttatcggtta ccttggtgaa gaaagggaaa acatcccaac agctggagca gcatcgccag
     7861 aagctagccc tgtacctgta gcaagtacct caaacgatga tggtaagagc gatgatgctt
     7921 ttgatatcgt tgtgattggt ggaggtcctg ctggttatgt tgcagccatt aaagctgccc
     7981 aactcggcgg taaggttgcc cttgttgaga aatctgaact tggtggaacc tgcttaaacc
     8041 gtggatgtat tccaaccaag acctaccttc atagcgctga aattattgaa aatatcggtc
     8101 atgctgcaaa tcgtggtatc gtgattgaaa atcctaactt cactgttgat atggaaaaac
     8161 ttttagaaac taaatctaaa gttgttaata ctcttgttgg tggtgttgca ggacttcttc
     8221 gtagctacgg agttgctgtt cataaaggaa ttggtacaat cactaaagac aagaacgtct
     8281 tggtaaatgg ttctgaattg cttgaaacca agaaaatcat tcttgctggt ggttcaaaag
     8341 tcagcaagat caacgtccct ggtatggaat ctccacttgt catgactagt gatgacattc
     8401 ttgaaatgaa cgaagtgcca gaaagccttg ttatcatcgg tggtggagtt gtcggtatcg
     8461 aactcggtca ggccttcatg acatttggtt caaaagtgac tgttatcgaa atgatggacc
     8521 gtatagttcc agctatggat gtggaagttt ctaagaacct tcgcttgatc cttgagcgta
     8581 aaggaatgac catcttgact ggtactaaac tgcaagaaat cattgaggaa aatggtcaac
     8641 ttcgtatcaa ggttgaagga aaagacgata tcatcgcaag caaagctctt ctttcaattg
     8701 gtcgtatgcc agaccttgaa ggtattggag aggttgagtt tgaattggat cgtggttgta
     8761 tcaaggtcaa cgaatacatg gaaacttcag ttccaggcat ttatgcacca ggtgacatca
     8821 acggtactaa gatgttggct cacgcagctt tccgcatggg tgaagtttcc gctgaaaatg
     8881 cccttaaagg aaatcatgca gttgccaaat tgaatttgac tcctgcagcc atctacactc
     8941 ttcctgaagt agcagcagta ggtttgacag aagaacaagc ccgtgagaaa tacgatgttg
     9001 ccatcggtaa gtttaacttt gctgctaacg gtcgtgctat tgcatctgac gcagctcaag
     9061 gtttcgtaaa agttatcgct gataagaaat acggagaaat ccttggtgta cacattattg
     9121 gtcctgcagc cgcagaatta ataaacgaag catcaagcat catcgaaatg gaaatcactg
     9181 ttgaggaaat gctgaagacc atccacgggc acccaactta ctctgaagtg atgtacgaag
     9241 cgtttgcaga tgttctagga atggccatcc attcacctaa gaaaaaataa aacgaagata
     9301 gattagactg gaaggttatt tccagtttct atcatataaa gtaaagggat atcatgaaat
     9361 acattatcaa tcattcaaat gacactgctt ttaatattgc cttggaagaa tacgccttta
     9421 aacacctttt ggatgaggat caaatcttcc tactttggat taacaagcca tctatcattg
     9481 ttggtcgtca ccagaacact attgaagaaa tcaaccgtga ttatgttcgc gaaaatggta
     9541 ttgaggtagt tcgccgtatt agtggtggtg gagctgttta ccacgattta aataacctca
     9601 actacacgat catctccaaa gaagatgaaa ataaggcatt tgacttcaag agcttctcaa
     9661 ctccggttat caataccttg gctcaactcg gggttaaagc tgagttcaca ggtcgtaatg
     9721 accttgagat tgatggtaag aagttctgtg gcaatgccca agcctatatc aacggccgta
     9781 tcatgcacca cggttgcttg ctctttgacg ttgatttgtc agtcctcgca aatgccctta
     9841 aggtttcaaa agataaattt gaatcaaaag gtgtgaaatc cgtccgtgcc cgtgtaacca
     9901 atattatcaa tgaattacca aaaaaaatca cagtcgaaaa attccgtgat ttactcttgg
     9961 aatacatgaa aaaagagtac ccagagatga ctgaatacgt cttttcagaa gaagaattgg
    10021 ccgaaatcaa tcgcatcaag gatactaagt ttggaacttg ggactggaac tatggtaaat
    10081 cacctgaatt taacgtccgt cgtggaacca aattcactag tggtaaggtt gaagtctttg
    10141 ctaacgttac tgaatcaaaa atccaagaca tcaagattta tggtgacttc tttggtattg
    10201 aagacgttgc tgcagtagaa gatgtccttc gtggggtgaa atacgaacgc gaagatgttc
    10261 ttaaggcgct aaaaaccatt gatatcacac gctacttcgc tggtattagc cgtgaagaaa
    10321 tcgctgaagc ggtagttgga taaatcaaaa agaagttggc caactaacca acttcttttt
    10381 ttgatattta tttacataat ttaaaatacg taaaatcata aactatccag agcattcttt
    10441 tgttcatcac taacaatatg ggtatagagg tcagtgactt gtgtgctagc atgtcctagt
    10501 tggtgactga ctaaaacttg tgatttagtc gcatcataga gcctagttgc tagtgtatgg
    10561 cgcagtttat ggggtgttac acgcacttta aaatcctctg agtatttagc aaccattttc
    10621 tcaacgctag aagcatcgat acgattagga acacctctgt agagagttaa aaaaagggct
    10681 gtatctgttt tttccgtttt atagcgttga ttccgaatgg ccagataatt ctctaaataa
    10741 ggtttagcaa aagcagcgac attgactgag tcacgtttgc caccttttcg agtaacatca
    10801 ataaccatca tttttagatt gagatctctt agatctagat taacagcttc agataagcga
    10861 acaccagatg ccaagagaag ggcaataatg gctaaatctc gttctttatt tttgttgaat
    10921 gatgagagag ctcgatttga aagctgttgt gggtgctctt gatcgatata agttagaaaa
    10981 ccttctgttt catcacctag aaagagtttt tgcttgatat ttttagctct ggcagcaagg
    11041 gtttctttct ttttcttggt tgaaactttt ttcattacat tacgatagaa ataaggttcc
    11101 ccctgatcgt tttcaacctc ttcggttaga tacttgtaaa gactagaaag tgctgataag
    11161 gttcgattga tagttgtctg tgaaacacct tgttttgttg tattagcatt cagcaaggga
    11221 cgttcacgta gataaaggat aaaggattcc atgtctttct tagacatatt ttccaaaact
    11281 gataaaggaa tatcggatat tttatcagcg tttgaaatac cagactccaa aacccagctg
    11341 aaaaatcggt catattcctt aaggtattcg tacagggttg taaaactgta gggcacagcc
    11401 agcttagatt ggtagtattc cagaacatac cagggcatga gttgttttag tttgtcgatt
    11461 cgttccagta aaatctcacg tttcatgtat tttctccata agtaagtata ctagtagtat
    11521 agcatagact tgtatatttt tcaagaaaat tatagttttt cagaaagatg ttataggagt
    11581 ttttcaaagt gataaaaaga cttagaccta caaacaaaac agaataagga aaataataaa
    11641 aatctcaccc actaaacttc tgttaatact acat
//
LOCUS       DAVMGZ010000056        11616 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000056 DAVMGZ010000000
VERSION     DAVMGZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.057"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            133..892
                     /locus_tag="VYQ93_001210"
                     /pseudo
     CDS             133..892
                     /locus_tag="VYQ93_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            932..1696
                     /locus_tag="VYQ93_001211"
     CDS             932..1696
                     /locus_tag="VYQ93_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV3335898.1"
                     /translation="MISLFGLVAAKPVQANTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLTFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1709..2386
                     /locus_tag="VYQ93_001212"
     CDS             1709..2386
                     /locus_tag="VYQ93_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3335899.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPFYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPGFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2396..3055
                     /locus_tag="VYQ93_001213"
     CDS             2396..3055
                     /locus_tag="VYQ93_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3335900.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALVEI
                     IRGGLNAVDKGQYEVGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLVKRWQQAA
                     E"
     gene            3350..4540
                     /locus_tag="VYQ93_001214"
     CDS             3350..4540
                     /locus_tag="VYQ93_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV3335901.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKIIHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(4861..5742)
                     /locus_tag="VYQ93_001215"
     CDS             complement(4861..5742)
                     /locus_tag="VYQ93_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV3335902.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLTDGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5883..7211)
                     /gene="vncS"
                     /locus_tag="VYQ93_001216"
     CDS             complement(5883..7211)
                     /gene="vncS"
                     /locus_tag="VYQ93_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV3335903.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENSLTFFIDFPKVVQD"
     gene            complement(7208..7864)
                     /gene="vncR"
                     /locus_tag="VYQ93_001217"
     CDS             complement(7208..7864)
                     /gene="vncR"
                     /locus_tag="VYQ93_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV3335904.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7913..7996)
                     /gene="pep27"
                     /locus_tag="VYQ93_001218"
     CDS             complement(7913..7996)
                     /gene="pep27"
                     /locus_tag="VYQ93_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV3335905.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(8176..9555)
                     /gene="vex3"
                     /locus_tag="VYQ93_001219"
     CDS             complement(8176..9555)
                     /gene="vex3"
                     /locus_tag="VYQ93_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV3335906.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9607..10254)
                     /gene="vex2"
                     /locus_tag="VYQ93_001220"
     CDS             complement(9607..10254)
                     /gene="vex2"
                     /locus_tag="VYQ93_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV3335907.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10267..11544)
                     /locus_tag="VYQ93_001221"
     CDS             complement(10267..11544)
                     /locus_tag="VYQ93_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3335908.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3266 a         2600 c         2197 g         3553 t
ORIGIN      
        1 atcgatttga ttgtcccgat cgatttttcc tgttattata tcactatagt gaattttttc
       61 agatatgatt tgacaagaac attcttttat cgtagaatca tgttataaaa tctaacacaa
      121 gggagatctc tcatggcttt agtagaattt aaaaacgtcg aaaaatatta cggagactac
      181 cacgcactcc gcaacatcaa tctccgtttt gaaaaaagga caagttgttg tcctgcttgg
      241 accttctggc tctgggaagt ccactcttat ccgtacgatc aatggtttag aggctgttaa
      301 caaaggaagt ctcctagtca atgggcacca agttgctggt gccagccaga aagatttggt
      361 acctcttcgc aaggaagtcg gcatggtttt tcaacatttt aacctttatc cacacaaaac
      421 agtgttagaa aacgtaacac ttgcacccat tgaagttcta ggaattgata aaaaagaagc
      481 tgaaaaaacc gcccaaaaat atctggaatt tgtaaatatg tgggacaaga aagattccta
      541 tcccgccatg ctatctggtg gacaaaaaca gcggatcgcc atcgctcgtg gtcttgctat
      601 gcatccggaa ctcctcctct ttgatgaacc aacatctgct cttgatcctg agactatcgg
      661 agatgttcta gcagttatgc agaaactggc gcatgatggg atgaacatga tcatcgttac
      721 ccacgaaatg ggctttgctc gagaggttgc ggaccgcatt atctttatgg ccgacggaga
      781 agttttagta gatatgacag atgtcgatag cttttttgac aatccaagcg aacctcgtgc
      841 ccaacaattc ctcagcaaaa ttatcaacca cgaaagtgac aaagtcaaat aaggaggcgc
      901 ctatgaaaaa aaattctttt tatcagcatt attgattagc cttttcggcc ttgttgctgc
      961 caaaccagtc caggctaata ctagtatcgc agacattcaa aaaagaggcg aactggttgt
     1021 cggcgtcaaa caagacgttc ccaattttgg ttacaaagat cccaagaccg gtacttattc
     1081 tggtatcgaa accgacttgg ccaagatggt agctgatgaa ctcaaggtca agattcgcta
     1141 tgtgccggtt acagcacaaa cccgcggccc ccttctagac aatgaacagg tcgatatgga
     1201 tatcgcgacc tttaccatca cggacgaacg caaaaaactc tacaacttta ccagtcccta
     1261 ctacacagac gcttctggat ttttggtcaa taaatctgcc aaaatcaaaa agattgagga
     1321 cctaaacggc aaaaccatcg gagtcgccca aggctccatc acccaacgcc tgattactga
     1381 actgggcaaa aagaaaggtc taacctttaa atttgtcgaa cttggttcct acccagaatt
     1441 gattacttcc cttcacgctc atcgtattga tgccttttcc gttgaccgct ctattctgtc
     1501 tggctacact agtaaacgca cagaactact agatgatagt ttcaagccat ctgactacgg
     1561 tattgttacc aagaaatcaa atacagagct caacgactat cttgataact tggttactaa
     1621 atggagcaag gatggtagtt tgcagaaact ttatgaccgt tacaagctca aaccatctag
     1681 ccatactgca gattaaggag gacaccccat gacagattta tcatcttgga cagcctattt
     1741 tcaggatttt ggacaatttt tcaatggttt cctctttact cttgccctag cgattggatc
     1801 ctttatcctc gccatggtct taggaatctt ctttggtgcc atgtcaacca gtaaacgtcc
     1861 aattttacgc attttagctc gcatctttgt tgaattttac caaaatactc ccctcttggt
     1921 gcagtttgtt attgtttttt atggcctacc tcttatcagt gaccatatca tcatgattcc
     1981 attttattgg acagccgttc tctgcgtggg actctatcac ggcgcttata tcgctgaggt
     2041 tattcgttca gggattcagt ctattcctag tggtcagatg gaggccgcct tgtcgcaagg
     2101 ttttacctat atcagtgcca tgcgcttgat tatcttgcct caggccttcc gcatcattct
     2161 ccctccattg accaaccaaa ttgttaacct catcaagaac acctctactg tagctatcat
     2221 ctctggagta gacttgatgt ttgtgactaa gtcttggtcg gctctcaacg gaaactatat
     2281 cccaggcttt ttaggtgctg ctcttctcta ctttgcccta tgcttccctg ttgcccagtt
     2341 tggtcgcaag atggagcaag ccaacaaaaa agcctattca ctttaggagg ttactatgga
     2401 atctatttta gaagttttaa ccccagataa cctagtcttt atctttaaag gatttggctt
     2461 gaccctctat atttctctga ttgccatcat cctctctact atcatcggta cggtgctagc
     2521 tgtcatgaga aatggcaaaa atcctgtctt gcgaatcatc tccagtattt atatcgagtt
     2581 tgtgcgcaac gttcccaacc ttctctggat ttttactatc tttttggtgt tcaaaatgaa
     2641 atccacacca gcaggtatta cagcctttac tctctttaca tcagcagcct tggttgagat
     2701 tattcgaggc ggtctcaatg ccgtagacaa gggacaatac gaagtaggaa tgtcacaagg
     2761 cttcacctca gcccaaatcc tctactacat cattctccca caagccatcc gcaaaatgct
     2821 accagccatc atttctcagt ttgttaccgt gattaaggat accagtctcc tctactctgt
     2881 tatcgcccta caagaactct ttggagccag ccaaattctc atgggccgtt atttcgaacc
     2941 agagcaggtc ttcagtcttt acatcctgat tgccctcatc tacttcagct ttaacctagc
     3001 aatttctagc ctgtctcata tgctagtcaa acgttggcaa caagctgcag aataatactc
     3061 ttcgaaaatc tcttcaaacc acgtcagctt taccttgccg tatatatgtt actgacttcg
     3121 tcagttctat ccacaacctc aaaacggtgt tttgagcagc ctgcggctag cttcctagtt
     3181 tgctctttga ttttcattga gtataaacag ccactctcca gttcaattgg agagtttttt
     3241 gatgatagta gatatttata gacctcaatt tgacaccata cattttctat gataaaatgt
     3301 aacaaattta tttacaacaa atcgaaatct aaatgaaatg gaattttcta tgaaatcagt
     3361 caaaggacta ctctttatca tagctagttt tatcttgact cttttgactt ggatgaacac
     3421 ttctccccaa ttcatgattc caggactagc tttaacaagc ctatctctga cttttatcct
     3481 agccactcgt ctcccactac tagaaagctg gtttcacggt ttggagaagg tctacaccgt
     3541 ccacaaattc acagcctttc tctcaatcat cctactaatc tttcataact ttagtatggg
     3601 cggtttgtgg ggctctcgct tagctgctca gtttggcaat cttgccatct atatctttgc
     3661 cagcatcatc cttgtcgcct atttaggcaa atacatccaa tacgaagctt ggcgatggat
     3721 tcaccgcctg gtttacctag cctatatttt aggactcttt cacatctaca tgataatggg
     3781 caatcgtctc cttacattta atcttctaag ttttcttgtt ggtagctatg cccttttagg
     3841 cttactagct ggtttttata tcatttttct atatcaaaag atttccttcc cctatctagg
     3901 gaaaattatc catctcaaac gcttaaatca cgatactaga gaaattcaaa tccatcttag
     3961 cagacctttc aactatcaat caggacaatt tgcctttcta aagattttcc aagaaggctt
     4021 tgaaagtgct ccgcatccct tttctatctc aggaggtcat ggtcaaactc tttactttac
     4081 tgttaaaaat tcaggcgacc ataccaataa tatctatgat aatcttcaag ccggcagcaa
     4141 agtaacccta gacagagctt acggacacat gatcatagaa gaaggacgag aaaatcaggt
     4201 ttggattgct ggaggtattg ggatcacccc cttcatctct tacatccgtg aacatcctat
     4261 tttagataaa caggttcact tctactatag cttccgtgga gatgaaaatg cagtctacct
     4321 agatttactc cgtaactatg ctcagaaaaa tcctaatttt gaactccatc taatcgacag
     4381 tacgaaagac ggctatctta attttgaaca aaaagaagtg cccgaacatg caaccgtcta
     4441 tatgtgtggt cctatttcta tgatgaaggc acttgccaaa cagattaaga aacaaaatcc
     4501 aaaaacagag cttatttacg aaggattcaa gttcaaataa gtaatcatac tctcctggga
     4561 tttcccagga gaatttttta tagccaaatg aaataagacc ggaacgatgg aattttggca
     4621 ttcaaatcaa ttgataagaa tattttatag tggagtgaaa taggatacga acaaattgat
     4681 taggaaaatc aaatgaattt atagaaatct tttagcagtt atgttgtcct attctagttt
     4741 caaaacgcta tagaagcagc attgtgctag tctagattca gtttactata ctaaaacgag
     4801 tagcttgaaa tcaaaaaacc caccctcaca ggcaggtttt atctgtatta ttcagctaga
     4861 ttatgcttta ccttctgaac cgaatacgtc gatacgttct tcaaccgatg cttggatagc
     4921 ttttacaccg tcagtcaaga atttacgtgg gtcgaagagt tttttcttgt cgtattctgc
     4981 ttcgtttgct tcgtagtcac gagcaaattt acgagttgcg ttagcgaatg cgatttggca
     5041 ttctgtgtta acgttaactt tggcaacacc aagtttgata gctgcttgga tttgctcatc
     5101 aggaatacct gatccaccgt gcaatacgat tgggaatcct ggaagagctt ctgtcaattt
     5161 ttgcaagtgg tcaaggtcaa gaccttccca gtttactggg taagggccgt ggatgttacc
     5221 gataccagct gccaagaagt cgataccagt ttcaaccatt gctttagcgt cttcgattgg
     5281 agccaattca cctttaccga tgattccgtc ttcttcacca ccgatagtac caacttcagc
     5341 ttctactgag atacctttag cgtgtgcttt ttcaacaact tctttagcca atttaaggtt
     5401 ttcttcaact ggaaggtgtg aaccgtcaaa catgattgaa gtataaccaa cttcgataca
     5461 ctcaagtgca tcttcgtagt gaccgtggtc aaggtggata gctactggta cagtgatacc
     5521 cattgattca acaaggttag cgatcaagtt gcgagcaact ttgtaaccac ccatgtattt
     5581 agcagcaccc attgaagttt ggatcaaaac tggagctttt ttagcttctg ctgcgcgcaa
     5641 gatagcttga gtccactcaa ggttgtttgt gttaaatcca ccaactgcat aaccgttgtc
     5701 acgggctgct tggacaaatt tttctgctga aacgattgcc attttatcag gcctcctgta
     5761 tatttttatg ggtcatccca tttacattgt tcattttatc actttttgcc aaaaaaatct
     5821 agtttttccc gcagtttcga ttgattttct tctaactcca tctatgtaaa ccctttctct
     5881 ccctagtctt ggacgacttt tggaaaatct ataaagaagg ttaaactatt ctcctccatc
     5941 tcgaaacgat aagctaattt ttcatgttct aatagactct taaccacaaa gagccccata
     6001 ccagacccct tgaccttgcg actggcattg tcagaaaaag actgggctag tttttcttgt
     6061 tcctctgagc tacagctatt ttcgataaaa agttctcctt ctctttctcc aattcgaact
     6121 aagccacctg gaacagagtg cttaatggca ttgctgatga gattagaaag aatcaacttc
     6181 ataactgatg ggtttagata agcctgctga tgggtcaaac tattgtctat ctggagctct
     6241 ctttccttgg ctagcaaggc ataatctttg accagatttt gcgtcatctg gaggaggtca
     6301 attgtttccc tatcatctcg caattcctgc acagaagaga gggaaagtat ctgcagaaca
     6361 tggtgattga gttcatccac aatccccaag gcaactccca gatactggtc tctatcctta
     6421 taacgaccga tattctctct catattttcg attaggattt tcaaactagc cagcggtgtt
     6481 ttcaattcat gagaagctcc tcgtaggaat tcgaccttca tcttctccag ctggagaatg
     6541 gcttcattct tttcatgcaa gtccgcaata acagtcaaga gatgctggta gaggctattg
     6601 atttgttcct tgagattacc tatctcatcc ttagaatcca cgcgcaatcg cacttgggaa
     6661 tccaggtcca tcatccgacg ggtcacccgc ttgatttcca aaatcggtgc aacaatagtc
     6721 cgagcgtaga tgtaggccac caaaagggaa atcagaaagg aggccagcaa ggtataggga
     6781 agaaactgga gactgatttg ctccgcttcc ttttgtaaat ccatggaagc tagaaactgg
     6841 agaatcatag taccaccgtc ttgcgttttc acctcgcgct cctcaataaa gagagaggtt
     6901 gtctggcggt ctgtgtccag aggaagactg tccttgactt ctaacttgtc ctcggtcatc
     6961 tcacctttga cggtcccctt gatatcacta gtctgggaat acaagtctaa cacttgctcg
     7021 atactctgcc tatctttccc ttctagggac tgggcaatgg ctgttgcctt ttgaccaatg
     7081 gtttcctgac gatgactcag ataagtcgaa ggaaaaagaa aataaatagc taaatgaagg
     7141 cagataacca gaacactaaa tatcgagaag gtatagataa atatctttgc aaataaacct
     7201 gttcgtttca ttttcgctcc aatttataac caacattgcg cacagtgagg atacaatcca
     7261 aatctagctt tttccgcaat tccttgatat aaacatcaat aacacggtca aagggaacct
     7321 catctgtcgc tttccagacg gcatcgataa tctgagatcg agtcaaggcc cggccttcat
     7381 ttttcactag atagtccaga atttccaact ctttggcatt gataggcact tcttgacctg
     7441 cgaggcttgc actgtagctt tcaaagtcca ccttggtatc cttgtaagaa aagattcgtc
     7501 ctgtatcgta gtagcgcttg aaaatcgcgt ccaccctcac ttttaaaagg gagagggaga
     7561 aaggtttttc cagatagcca tctgccaaag aggcaaaggc actcatcttg tattcctcat
     7621 cttgaaaagc tgtcaacatc aagacaggaa cctgactggt tttacgaatc tcagctagga
     7681 cttctaagcc gttgagcttg ggcatctgga tatccagtaa aaccagggcc acctcatagc
     7741 tagaaaattg ctccagagct tcctgaccgt ccgctgcctc aatagtttca tagccacaat
     7801 ccgtcaaata atcactgacc ccctcacgga tcatctcttc atcttctaca attaaaattt
     7861 tcatacttta actgctctct attttttatt tttcttagaa taaataccta ccctattttc
     7921 tattatagtc tcttgctggc cttttgtctg caagcaactg accactagat aaaacgttgt
     7981 gaaattcctt tctcataaat tccataactt tagtatatta tatttaagca ctaaagtaca
     8041 aagaaagcaa ctgaaagcaa tgattttcac cactgctttc ggatttattt tgaattgtta
     8101 aatagccatt cctatccact attcttgaat agaaacacaa gatgcaatct ttattctaga
     8161 ctcatttttt caaatttatt caccatccag caagagctct tttggttgtt ttctaaggag
     8221 attgcttgaa gcaagcgcca taacgagaac cactagaacc aaggcaagga caaaaatgat
     8281 gataaagtct gatgtctgaa tggaaatgtc taggctcgac aaggtcttgc taaagccatc
     8341 tacttctgca ccaccaccaa ggttagaggc ttgagccgcc ttactagcct gtttggcaac
     8401 acctgaagtc acattggcaa ggacagtgtt tccaattgca cgggcagtgt aattagctag
     8461 gaagtaagca gaaactagag cagggatagc aatcaagata gattcggtga tgaattgacc
     8521 caagatactt gcctgcttga ggccgataga gaggagaatt cccacttcct tgcgacgggc
     8581 gttgatccaa aggctgagca agagggcaag gaggagaact gagaagctca agctacccca
     8641 gaagaggagg ttggccatct tgtacatacc agagatagat tgctcaagag ctgggtagtt
     8701 agaggagctc ttgacgagtg tgtagctctt ccagttgata ccactgatgc cattcaactc
     8761 tttcataaca tcatccaagt tcttgtctgc tgttacaaag aaggttgcgt ccccataaat
     8821 ggctgtgtct tctgtgtatc cataaagttt tgcagcagtg tgaatgtctg taatagctgt
     8881 gttttcgtaa agttcttgtg agtaggttac tgctgactta ttatgaccat caaagagtcc
     8941 cttgattgtc acttcaactg tttccttggc tcctttttca ttatctgcat cgtagatatt
     9001 agagtccagt ttaaccttgt cccctacttt ccagccgtgt ttggctgcca agtccttgtg
     9061 caagaggatt ttatccttgt cgtcgttggt taagtgctct ccttcgacta gtttataaga
     9121 accagagaca aacttgtctt ctttagagga gtcattgaca cctgtaatca tcaagctact
     9181 tccaaaacgc ttggcacgat cagcagtgag attcttcttg gtttctggcg tttcaatcag
     9241 gtcatatcca gtcaaatctc cgatagcgtt gatacgtttg acataagact caatggcctt
     9301 gttttcggtg atttttttga tgtcttcacc cttgatattc ccagcaccac gaggcgttcc
     9361 ttggttgacg cgacgattga tttgcatgga gaagctattg gtgatatttt taaaggtctc
     9421 ctgagaagcc ttggcagtag ctcccttgat tgacaagccg accaaactca agctcgccat
     9481 gaggagaata atcaggaaga tgacaatcga tttgaaaaac ttccttgtaa cataggcaaa
     9541 tgcgttgtgt aacatagatt ccctttctag attttgtttt aatcattcta ttaaaataag
     9601 ctcaaattat ttactagtat tgcgcgtttc agtcagtttc ttatccttta attcaagtgt
     9661 aatatctgac gcttgtgcca cttctttact gtgagttacg acaatcacac atttacctgt
     9721 tttctgggca agtgatttga gtagttcgac aatatctcca gcagttttag gatccagatt
     9781 tcctgttggc tcatcagcta gaataactgg agcttctgag accaaactgc gagcaatggc
     9841 aacacgttgc tgttgaccac ctgataactg gagaacattc cgcttgatct ggctttcatc
     9901 caaaccaagc tcaagaagtg tatccttgct tgcctttttg ttgaccaagc ggatattttc
     9961 cagcggagaa agataatcta tcaagttata attttgaaag accagggaaa tatggtgcat
    10021 gcgatggtaa gaatagccct tcttacgaat atcctctcct tgaaaaagga tagaaccttc
    10081 aacaggacta tctagaccag caagtaggga caagagtgtg gattttcctg ctcctgactc
    10141 cccaataata ctgtaaaatt ttccgggttc aaaattataa tcgatctgat ataggactgc
    10201 ttcagcagta ttcttataac ggtaggtaac atcttgtaat tgtaataaag tcatgatttc
    10261 tccttcttaa ctaatagatg ataaaatttc tttcggtgat tttctaaata agaataggaa
    10321 acaaagggct acagataagc aactaagcag aactagaaaa acataggatt ctgcaaaaga
    10381 taagatgcta gttgataaac tgcttgcttt ggctagtgta tcttgtaagc ttgcctgatc
    10441 tccacttgct agtagagttt ggagtaggta agttgtgatt ccgtttcctg caacaaatgc
    10501 tggaagcaaa gctccaagag ataccaaaac tacctctaaa cagaattgta ggaagatcga
    10561 gctcttgcct tttccaagtg caagtaaaat ccccacttca tagacccgtt ctctcaacca
    10621 gagagacaaa accagaatta aggctccagc tcctgctatc aacatcccat aaaggaagat
    10681 ggtcaggaag gtttggaaag ttgcaactga gtctttgatt tgttcaaaag ccttgttttc
    10741 cttttcgact tggtagcctt gattttccaa ggccaagttt tctacctgct tcatgagtcc
    10801 gtccatttcc ttaggatttt ctacatagaa gcgtgctgca ctgacttgag cttcactatt
    10861 gcccaaaagg gtttggctac tttcatagtc tgtaaagact tgattttcac tgaagtcaga
    10921 agacaagcct gtgaatttct cttgtttttt accagaaaag atgccaataa tctcaaactc
    10981 tactgtttgt ccttttccag attcagactg accagcatcc aagccaatct tgtcatgaag
    11041 tgaaagaccg ttcttcttag ccaattcttc gtggataagg attttcttgg aatccccttt
    11101 ttgaaggtgt cgcccttctt ttagattgaa agccgaactg gtaaaggtta catccttgga
    11161 tgaatcctca agagccgtta agctaaccaa gttattgtct gcagctgata aatcatcacg
    11221 ctccacgctc tgctcgccag tcactgcttc cttgtctttt agttttgcga ccgtctcaag
    11281 ttcaggagag acattttcca gccccttaat cttgcttaca gatgctaggt ctgacaactt
    11341 gaatgtctga ccattctcta tcttcttaat agaaaaagat gtattgagtg atttataaag
    11401 attgctttct actgttttgt tggacttcat cagagtcaaa caggctgaaa ttccggccaa
    11461 taagaccaat aaaatcagaa ataaaataaa acttctcagt cgctttctgc tgacataagc
    11521 ccaagatctt tggattggat tcatttgtca cctccatatt tgtaagacta ttataaaacc
    11581 caaatatgaa atgtttatga aatacgaaaa aaaaaa
//
LOCUS       DAVMGZ010000057        11540 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000057 DAVMGZ010000000
VERSION     DAVMGZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11540
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.058"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..273
                     /locus_tag="VYQ93_001222"
     CDS             <1..273
                     /locus_tag="VYQ93_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV3335909.1"
                     /translation="PEKPKPEVKPQLEKPKPDNSKPQADDKKPSTTNNLSKDKQPSNQ
                     ASTNEKATNKPKKSLPSTGSISNLALEIAGLLTLAGATILAKKRMK"
     gene            complement(492..1472)
                     /gene="dusB"
                     /locus_tag="VYQ93_001223"
     CDS             complement(492..1472)
                     /gene="dusB"
                     /locus_tag="VYQ93_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV3335910.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWANPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKV"
     gene            complement(1459..2331)
                     /gene="hslO"
                     /locus_tag="VYQ93_001224"
     CDS             complement(1459..2331)
                     /gene="hslO"
                     /locus_tag="VYQ93_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV3335911.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2496..3923
                     /locus_tag="VYQ93_001225"
     CDS             2496..3923
                     /locus_tag="VYQ93_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3335912.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPTFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            4082..5590
                     /gene="glpK"
                     /locus_tag="VYQ93_001226"
     CDS             4082..5590
                     /gene="glpK"
                     /locus_tag="VYQ93_001226"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV3335913.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5632..7458
                     /gene="glpO"
                     /locus_tag="VYQ93_001227"
     CDS             5632..7458
                     /gene="glpO"
                     /locus_tag="VYQ93_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV3335914.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPAANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVKAALAQNDLAELKN"
     gene            7526..8230
                     /locus_tag="VYQ93_001228"
     CDS             7526..8230
                     /locus_tag="VYQ93_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV3335915.1"
                     /translation="MMSELFGEFLGTLILILLGNGVVAGVVLPKTKSNSAGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8527..8730
                     /locus_tag="VYQ93_001229"
     CDS             8527..8730
                     /locus_tag="VYQ93_001229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335916.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(8817..9404)
                     /locus_tag="VYQ93_001230"
     CDS             complement(8817..9404)
                     /locus_tag="VYQ93_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335917.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            10508..>11540
                     /gene="ltrA"
                     /locus_tag="VYQ93_001231"
     CDS             10508..>11540
                     /gene="ltrA"
                     /locus_tag="VYQ93_001231"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEV3335918.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAASKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKFKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERL"
BASE COUNT         3441 a         2240 c         2617 g         3242 t
ORIGIN      
        1 ccggaaaaac caaaaccaga ggttaaaccg cagctggaaa aaccaaaacc agataatagc
       61 aagccacaag cagatgataa gaagccatca actacaaata atttaagcaa ggacaagcaa
      121 ccttctaacc aagcttcaac aaacgaaaaa gcaacaaata aaccgaagaa gtcattgcca
      181 tcaactggat ctatttcaaa tctagcactt gaaattgcag gtcttcttac cttggcgggg
      241 gcaaccattc ttgctaagaa aagaatgaaa tagtgattga ttcattcaaa aatgtttgta
      301 aaaagtattc ggcttaaaag aaaagagtct gggataaaaa gattttgatt ttaggaattc
      361 tttattataa atttttaaaa tcgatagatt agacaaaaaa gcgaacaaga cagaattctg
      421 agtgtcagat aactgatttt gttcgctttt tatatttaat attatacttt tgtcctaggc
      481 tcttttttgc attatacttt ttctaattgc aagagggctt caatctctgc tagggtgcta
      541 gcttgcgaaa tggctccacg gagtttggca gcgccagatg ttccacggag atagtgagga
      601 gcgaggccgc ggaattcacg aactgcgacg ttttctcctt tgaggttaat caatcgtttc
      661 aagtgttcgt aggcgatctt catcttgtct tcaaaggtca aatcaggtag gatttctcct
      721 gtttcaaagt aatggttgat ttggttgaag aggtaaggat ttcccatggc agctcggcca
      781 atcatgactg cgtcagcacc aacttcttcg atgcgttgct tggcttcttg gacagtacgg
      841 atatcaccgt tggcgatgaa tggaatcttg gttagagctt gggcaacctt gtaaagggtc
      901 tcaaggtctg cgtggccagt atacatttgt tcacgggtac ggccatgcat ggcgagggca
      961 gaaacacctg cagcctcagc agcgagagca ttttctactg ccagagatgg gttcgcccag
     1021 ccggtacgca ttttgacagt aagtgggata tcaaggacag actggacctt gttgatgata
     1081 gagtagatct tgtcaggatc cttgagccac atagcaccag cttcgttctt cacgattttg
     1141 ttgacagggc agcccatgtt gatatcgacg atatcggtct tggtgttttc ttggatgaat
     1201 tctgctgcgc gtgctaggct gtcttcatcg ctaccaaaaa gttggataga gacagggttt
     1261 tcgccctcat cgatatgaag catgtgcagg gttttttcgt tgttgtattg gattcccttg
     1321 tcagagacca tttccattac aacgagtcca gctccgagct cttttgcgat ggtacgaaag
     1381 gctgagttgg tcacgccagc cataggcgct aaaacggtac gattgggaat ctcaacattg
     1441 ccaatcataa aaggtgtatt aagatttgtc acgaatgagt tcctccaggt ccttttcatc
     1501 aaagttgtaa gtagtttggc agaattgaca agtgatttct accccgtggt cttcctcttt
     1561 catttcctgt aagtctgagc ttggaaggct ggcaagagcg ttcataaagc gttcatggct
     1621 acagtcacat tggaaacgga tttcttcttc agaaagacgc ttgtaggctt cgtccccgta
     1681 gatagccttg aggagggctt cgatatggtc gtcgctttcg agaagagtag agatagctgg
     1741 catttcttgg atgcgttttt caaagcgagc aatctcttct ttcttggctc ctggcaagac
     1801 ttgaactagg aaaccacctg caaccttgac cttgtcttcc tcgtccaaaa ggacattgag
     1861 gccgaccgct gaaggcgttt gttggctttc agtaaggtaa aaggcaaggt cttcaccgat
     1921 ttctccagag atgaggggag ttatagagtt gtaaggattt ccagtaccgt agtctgtgat
     1981 aacgaggaat tgaccatttc caacaaaagg tccgactagg acttcaccag tcgcagtctt
     2041 tttgatgtca acaccaggat tttgaacgta gcctttgacg ttccccttgg tatcagcgac
     2101 ggtgataata gcacctagag agctagagcc caacacctta actgtaagtt tggtatttcc
     2161 tttttcattg gctgcgagaa tctggctagc gataagagtt cgaccaagcg ctacagttga
     2221 gctagcttgg gtttgatgtt tttcttgagc agtgcggacg gtttccgtgc tatcaaggac
     2281 aaaagcacga aaggctccgc tttctgatat agttttaata attttatcca tagctactat
     2341 tttagcataa aaatgcccaa agggggagcc gtgtgtttac tgattttcag gataatggac
     2401 caggaaatca gcatgaaaat aaaaagagaa acagattatt ttagcatttg taagatttat
     2461 gctatgctta aggtagaaaa tgaaaggggt aacaaatgta tttaggagat ttgatggaga
     2521 aagccgagtg tggtcaattt tcaatccttt cctttctatt acaagagtct cagacgaccg
     2581 tcaaggctgt aatggaagaa acaggatttt caaaagcaac cctaaccaaa tatgtcaccc
     2641 tgctcaatga caaggctttg gatagtggct tagagctggc tattcactca gaagatgaaa
     2701 atctgcgtct gtctatcggt gcagctacca aggggagaga tattcggagc ttgtttttgg
     2761 agagtgctgt taaataccag attttggttt atcttctcta ccaccaacag tttttagccc
     2821 atcagctggc tcaagaattg gtgattagcg aggctacgct tggtcgtcac ttggctggtt
     2881 taaatcagat tttgtcagaa tttgatttat ccatccaaaa tggacgttgg cgaggtccag
     2941 agcatcagat tcgctatttc tatttctgtc ttttccgaaa ggtctggtcg agtcaggaat
     3001 gggaaggtca catgcagaaa ccagagagaa aacaggagat tgccaattta gaggaaatct
     3061 gcggtgcaag tttgtctgtg gggcagaaat tggacttggt tctctgggct cacatcagtc
     3121 aacaacgtct tcgggtcaat gcttgtcagt ttcaagtcat agaagagaaa atgcgagggt
     3181 attttgacaa tattttctac cttcgtttgc tgagaaaggt tccgtccttt tttgctgggc
     3241 aacatattcc actaggagtt gaggatggtg agatgatgat attcttctct tttctcctat
     3301 ctcatcgcat tcttcctctt catactatgg agtatattct tggttttgga gggcagttgg
     3361 cagatttact gacgcaattg attcaagaaa tgaagaagga ggaactattg ggggattata
     3421 cagaggacca tgtcacctat gaactcagtc agctttgtgc tcaagtctat ctctataagg
     3481 gctatatttt acaggatcgc tacaagtacc agttagagaa tcgtcatcca tatttactga
     3541 tggaacatga ttttaaagag acagcagagg agatttttca tgctctacct acttttcaac
     3601 aggggacaga tttagataag aagattctct gggaatggct ccagttaatc gaatatatgg
     3661 ctgaaaacgg tggccagcat atgcggattg gtctggattt gacatctggt tttcttgtct
     3721 tttcaaggat ggcagccatt ttgaaacggt atttggaata caatcgtttt attaccattg
     3781 aagcttatga ccctagtcgg cattatgatt tgctggttac caataacccg attcataaga
     3841 aggaacagac accagtctat tatttaaaaa atgacttgga tatggaggat ttggcaggga
     3901 ttcgtcagtt attattcact taaaaggctt ggttgttcca ggtctttttt gtgaaattca
     3961 cacaatctcc tcacattttt ttaaaaatta aaaaaagttg ataaacaaga aagcgcttta
     4021 ttttgtatac tagtaagtgt aaagaggaaa cacctcaaga tttttatcag gaggacagaa
     4081 catgtcacaa gaaaaataca tcatggccat tgaccaggga actacaagtt ctcgtgccat
     4141 cattttcaac aaaaaagggg aaaaggttag ctcgagtcaa aaagagttta cccagatttt
     4201 ccctcaggca ggttgggttg agcacaatgc caatgaaatt tggaactctg ttcagtcagt
     4261 tattgcgggt gctttcatcg aaagtggtgt caagccaaat caaatcgagg caatcgggat
     4321 taccaaccaa cgtgaaacaa cggttgtctg ggataagaaa acaggacttc ctatctacaa
     4381 tgctatcgtt tggcagtcac gccagacagc acctttggct gagcaactaa aaagccaagg
     4441 ttatgtggaa aaattccatg aaaagactgg tttgattatt gatgcttact tctctgctac
     4501 caaggttcgt tggattttgg atcatgtaga aggtgctcaa gagcgagcag aaaaagggga
     4561 attgctcttt ggtactatcg atacttggtt ggtttggaaa ttgactgacg gtgcggctca
     4621 cgtgactgac tactcaaatg cagctcgtac catgctttat aacattaaag aactcaaatg
     4681 ggatgatgag attttggaaa tccttaacat tccgaaggct atacttccag aagttcgttc
     4741 taactccgaa atctacggca agacagctcc attccatttc tacggtggag aggtgccaat
     4801 ctcaggtatg gctggggacc aacaagcagc cctctttgga cagttggctt ttgagccagg
     4861 tatggttaag aatacttatg gaacaggctc tttcatcatc atgaatactg gggaagagat
     4921 gcagttgtct gaaaacaacc tcttgacaac cattggttac ggaatcaacg gtaaggttta
     4981 ttatgccttg gaaggttcta tattcatcgc aggaagtgct attcagtggc ttcgtgacgg
     5041 tcttcgcatg gttgaaaatt caccagaatc tgaaaaatac gctcgtgatt ctcacaacaa
     5101 cgatgaagtt tatgtcgttc cagcctttac aggtctaggc gctccatact ggaaccaaaa
     5161 tgctcgtggt tccgtctttg gtttgactcg tggaacaagc aaagaagact ttatcaaggc
     5221 gactttgcaa tctattgctt atcaagtgcg tgacatcatc gacaccatgc aagtggatac
     5281 tcagaccgcc attcaagtac tgaaggtgga tggtggtgca gccatgaaca acttcctcat
     5341 gcagttccag gcggatattt taggcattga cattgcacgt gctaaaaact tggaaacaac
     5401 agctctagga gcggccttcc tagcaggttt gtcagtaggg tactggaaag acttggacga
     5461 gttgaaactc ttgaacgaga caggagaact ctttgagcca tctatgaacg aatctcgcaa
     5521 ggaacaactc tacaagggct ggaagaaggc tgtgaaagca actcaagtct ttgcggaagt
     5581 agacgactaa tactggcaga ataaagcgat ttatttagaa agtgtgtaaa tatggaattt
     5641 tcaaagaaaa cacgtgaatt gtcaattaaa aaaatgcagg aacgcaccct ggacctcttg
     5701 attatcggtg gaggaatcac aggagctggt gtagccttgc aggcggcagc tagcggtctt
     5761 gagactggtt tgattgaaat gcaagacttt gcagaaggaa catctagtcg ttcaacaaaa
     5821 ttggttcacg gaggacttcg ttacctcaaa caatttgacg tagaagtggt ctcagatacg
     5881 gtttctgaac gtgcagtggt tcaacaaatc gctccacaca ttccaaaacc agacccaatg
     5941 ctcttaccag tttacgatga agatggagcg acctttagcc tcttccgtct caaagtagcc
     6001 atggacttgt acgacctttt agcaggtgtg agcaacacgc cagctgctaa caaggttttg
     6061 agcaaggatc aagtcttgga acgccagcca aacttgaaga aggaaggctt ggtaggaggt
     6121 ggagtgtatc ttgacttccg taacaacgat gcgcgtctcg tgattgaaaa catcaaacgt
     6181 gccaaccaag acggtgccct cattgccaac cacgtgaagg cagaaggctt cctctttgac
     6241 gaaagtggca agattacagg tgttgtagct cgtgatctct tgacagacca agtatttgaa
     6301 atcaaggccc gtctggttat taatacaaca ggtccttgga gtgataaagt acgtaatttg
     6361 tctaataagg gaacgcaatt ctcacaaatg cgcccaacta agggagttca cttggtagtg
     6421 gattcaagta aaatcaaggt ttcacagcca gtttacttcg acacaggttt gggtgacggt
     6481 cgtatggtct ttgttctccc acgtgaaaac aagacttact ttggtacaac tgatacagac
     6541 tacacaggtg atttggagca tccaaaagta actcaagaag atgtagatta tctacttggc
     6601 attgtcaaca accgcttccc agaatccaac atcaccattg atgatatcga aagcagctgg
     6661 gcaggtcttc gtccattgat tgcagggaac agtgcctctg actataatgg tggaaataac
     6721 ggtaccatca gtgatgaaag ctttgacaac ttgattgcga ctgttgaatc ttatctctcc
     6781 aaagaaaaaa cacgtgaaga tgttgagtct gctgtcagca agcttgaaag tagcacatct
     6841 gagaaacatt tggatccatc tgcagtttct cgtgggtcta gcttggaccg tgatgacaat
     6901 ggtcttttga ctcttgctgg tggtaaaatc acagactacc gtaagatggc tgaaggagct
     6961 atggagcgcg tggttgacat cctcaaagca gaatttgacc gtagctttaa attgatcaat
     7021 tctaaaactt accctgtttc aggtggagaa ttgaacccag caaatgtgga ttcagaaatc
     7081 gaagcctttg cgcaacttgg agtttcacgt ggtttggata gcaaggaagc tcattaccta
     7141 gcaaatcttt acggttcaaa tgcaccgaag gtctttgcac ttgctcacag cttggaacaa
     7201 gcgccaggtc ttagcttagc agacaccttg tcccttcact atgcaatgcg caatgagttg
     7261 actcttagcc cagttgactt ccttcttcgt cgtaccaatc acatgctctt tatgcgtgat
     7321 agcttggata gtatcgttga gccagttttg gatgaaatgg gacgattcta tgactggaca
     7381 gaagaagaaa aagcaactta ccgtgctgat gtaaaagcag ctcttgctca aaacgactta
     7441 gcagaattaa aaaattaaga aaaaataaaa gaggtggagg gtagcaatcc ttgtcgcccg
     7501 cccctttttt aatggagaca gaaaaatgat gagtgaatta tttggagaat ttttagggac
     7561 tttaatcctg attcttctag gaaatggtgt tgttgcaggt gtggttcttc caaaaaccaa
     7621 gagcaacagt gcaggttgga ttgtgattac tatgggttgg gggattgcag ttgcggttgc
     7681 agtctttgta tctggcaagc tcagtccagc tcatttaaac ccagctgtga ccatcggtgt
     7741 ggccttaaaa ggtggtttgc cttgggcttc cgttttgcct tatatcttag cccagttcgc
     7801 aggggccatg ctgggtcaga ttttggtttg gttgcaattc aaacctcact atgaggcaga
     7861 agaaaatgca ggcaatatcc tggcaacctt cagtactgga ccagccatca aggatactgt
     7921 atcaaacttg attagcgaaa tccttggaac ctttgttttg gtgttgacaa tctttgcttt
     7981 gggtctttac gattttcagg caggtatcgg aacctttgca gtgggaactt tgattgtcgg
     8041 tatcggtcta tcactaggtg ggacaacagg ttatgccttg aacccagctc gtgaccttgg
     8101 acctcgtatc atgcacagca tcttgccaat tccaaacaag ggagacggag actggtctta
     8161 cgcttggatt cctgttgtag gccctgttat cggagcagcc ttggcagtgc ttgtattctc
     8221 acttttctag tttatactct tcgaaaatca aattcaaacc acgtcagcgt cgccttaccg
     8281 tactcaagta cagcttgcgg ctagcttcct agtttgctct ttgattttca ttgagtatta
     8341 gaaaacaatt atgttgatag agcttgggca agagcccaat ttcagcaaaa aatgaagtaa
     8401 atcttctcat aataaaacgc atcatatcaa gcacgaaaat tccacgaggt caactacagt
     8461 cagaaagctg aacaacaagc caaaacgccc aaaaaaggcg gcaaaaagca agcacctgca
     8521 agcaacgtgc cgaaatggtc aaatcctgat tatgtcaacg aattagaccc aaaaatcgtt
     8581 gatatgctag tagaatttca caagtcacaa ggcactttgg aaactcccga ggcgcaagca
     8641 gaaatcgccc aaaaacgtga agaaatcgag caaaggagag ctgagcttga gggtaaaaaa
     8701 caagagcttt tgaaccgctt gaacaaatag agtttcgcaa gtattatgct tacaaattac
     8761 ttgagcaatt aactaaaata taaaccctgc ctttatatct atgcagggtt tatattttag
     8821 aaattcacgt aggttgttac ggtttttaca tacccagtat agtttgagtt tctatagtat
     8881 tcagtgataa acttccattt tctttgagca acatggatat aagtacttgt tatgtagtat
     8941 ggatatgggc tttgtgaatc caagtaagac tgataagctt gtataccaaa atatgctcca
     9001 ccaattattg caccccatgg accccccaat aaagcaccta tcctaccaat catataactg
     9061 attccagcac cagtcatgaa gttagcgaat gtgttagctt gtttattccc atgtattgtg
     9121 ttgacgtaat tccaaacatt aggatcgtat gatctaaaag atatatttag gtcgatttca
     9181 ttcttttgat aagccatata aaatgcccca ttgatataga cgccgtcagc acgtcgttca
     9241 atagtgtcta cacttccatc tggattgaca acctcaagaa cttcatcgct taaaatattt
     9301 acttgcgtat ctccgaaccg cactgatgag ccattctcaa actgagcctc accagataca
     9361 actttagagt ttgccgataa gctatcatca gcaaaaacaa acaagcgacg gggaaatgct
     9421 agacatacag aaaacagaca taactagcaa acacatgcat ttaaacatct tagacataac
     9481 ggaaactcct ttgtattttt gatttttttc aacttttatt atacaataaa accaaataaa
     9541 aagaaagcgg taacaatatg cttaatgcga aaatttttta tatattttta tgtttgatcg
     9601 ttatcgaaac tacaggcttg ttgttgttga aaagaggtct cgaaatgggt tatttagaca
     9661 cagaagctat tatcctcgca gttttttcat ttgcttttta caacctatgt tcattcgctt
     9721 gggtctgctc tacaataaaa aataaataga cgtattttca aaaaaaaaaa aaaaatgcat
     9781 atttatctta gcaaaacgac gatttaaatc gtcgtttttt tgtagtacga cgggcatgtc
     9841 gtatatctga ggtgtaagtc ctcggtgggc acctgctacc ggtgaaccca atagcgattc
     9901 ccaagcctga ctatcgtgag gtagcaggga gaggaaggga tagcgaaatc gtggctctac
     9961 gaacaggaac gtgatagtaa ggcgtatata gcggataagg aggcttcaaa ctctaaagtc
    10021 caaaaaggta gtcgtaacct atatgtgtaa atcacgagag taattgaatt cggactaagg
    10081 tttgtgtgaa aaagataaat ctttctagag tctaaagact ctgcgtcaga tttcctattt
    10141 tcactgtaac cttttaacgt cctcatatct tgtataaacg aggaaagatg tacgacttat
    10201 tccgtgaggt ttcatgagcg ctgaaagcgt agtaacaacg aatcatgaga agtcagccga
    10261 gcccatagta gtgaggaaac ttctgtagtg gaagtggagc gaaggggtga atactcaaac
    10321 agtctgggga gagactgttt gaggtctgtc gctagaaaga gaaaacgaca gatcgaagta
    10381 atcctacttc acttgtgtct gtaaaatgag tggtctgata gaactggact ttgacacgta
    10441 atgactagat gaaggttcac caatataaga tgtccctcag gcaccaaaac aagaaaggaa
    10501 tacgcacatg tcaaaactgc tagataagat attatcacgc gaaaatatgc tggaagccta
    10561 caatcaagta aaatccaata aaggctcagc tgggattgat ggaatgacta tcgaagagat
    10621 ggataattat ctcagacaaa actggcgctt gactaaggaa ctgataaaac agagaaaata
    10681 taagcctcaa ccagttctta aagttgagat acctaaacca gacggaggca tccgtcaact
    10741 aggaattcca acagttatgg atagaatgat tcaacaggcc attgtccaag tcatgagccc
    10801 catttgtgaa ccccatttct cagatacgag ttatggtttc agaccaaata ggtcatgtga
    10861 aaaagccatc atgaagctct tagaatactt aaatgacggc tatgagtgga tagtggatat
    10921 agacctagag aaatttttcg atacagttcc tcaagataga ttgatgtcct tagtacataa
    10981 cattatcgaa gacggagata cggaatcctt gattcgtaag tatcttcatt caggtgttat
    11041 cattaatggt caacgttata aaacactagt tggtacacca cagggaggaa atttatctcc
    11101 tctcttatcc aatatcatgc ttaatgaatt ggacaaggaa ttagaaaaga ggggacttcg
    11161 atttgtgcgc tacgcagatg attgtgtgat tacggtcgga agcgaggcag cctctaagcg
    11221 tgtgatgtat tcagtcagtc gttttattga gaaacggcta ggtttgaaag taaacatgac
    11281 caagactaag attaccagac caagagagtt gaaatatcta ggttttgggt tctggaaatc
    11341 atcagatggt tggaaaagcc gtccacatca agatagtgtt cggagattta agtttaaatt
    11401 gaagaaacta acacagagga aatggagtat agacctaaca agacgtattg agcaactgaa
    11461 tttgtctatt cgaggatgga taaactattt ctcattggga aatatgaaaa gtatagtcgc
    11521 cagcatagat gagcgcttgc
//
LOCUS       DAVMGZ010000058        11033 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000058 DAVMGZ010000000
VERSION     DAVMGZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.059"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            24..380
                     /locus_tag="VYQ93_001232"
     CDS             24..380
                     /locus_tag="VYQ93_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335919.1"
                     /translation="MSGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGATAGAFSGV
                     ALLLNALKIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGWVGGILVII
                     GGSLFLATLKKFKSEE"
     gene            574..864
                     /locus_tag="VYQ93_001233"
     CDS             574..864
                     /locus_tag="VYQ93_001233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335920.1"
                     /translation="MIESIRTYADYIDLYKNIFDDYFTKAEEGFKGTAMENNDSFTKL
                     KESTQKLFDAQKKRLNNEDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
     gene            915..1607
                     /locus_tag="VYQ93_001234"
     CDS             915..1607
                     /locus_tag="VYQ93_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV3335921.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1660..4071
                     /locus_tag="VYQ93_001235"
     CDS             1660..4071
                     /locus_tag="VYQ93_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV3335922.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            4321..5022
                     /locus_tag="VYQ93_001236"
     CDS             4321..5022
                     /locus_tag="VYQ93_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3335923.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5024..7732
                     /locus_tag="VYQ93_001237"
     CDS             5024..7732
                     /locus_tag="VYQ93_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV3335924.1"
                     /translation="MIKRKTYWKDLIQSFTDSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDREIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQAQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGRAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7828..8319)
                     /gene="tpx"
                     /locus_tag="VYQ93_001238"
     CDS             complement(7828..8319)
                     /gene="tpx"
                     /locus_tag="VYQ93_001238"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV3335925.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8442..9371)
                     /gene="psaA"
                     /locus_tag="VYQ93_001239"
     CDS             complement(8442..9371)
                     /gene="psaA"
                     /locus_tag="VYQ93_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV3335926.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9403..10251)
                     /locus_tag="VYQ93_001240"
     CDS             complement(9403..10251)
                     /locus_tag="VYQ93_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV3335927.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10248..10970)
                     /locus_tag="VYQ93_001241"
     CDS             complement(10248..10970)
                     /locus_tag="VYQ93_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV3335928.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3212 a         2061 c         2530 g         3230 t
ORIGIN      
        1 tgaaattaaa aacacttgct ttaattagtg gtatcgtcgg tcttgtggga ggaattttac
       61 ttcttattgg tccttttgtc ttgttgggaa cagcggtaaa cacagttgct acaactctta
      121 atggaggagc tactgcaggg gctttttcag gtgttgcctt actcttgaat gccttgaaga
      181 ttgcaaatct tgttcttggt atcattgcta ttgtttacta taaaggagat aatcgtgtag
      241 gtgcagctcc gtctgtacta atgattgttt ttggtggagt tagtctcatt ccattcttag
      301 gatgggttgg ggggattctt gttattatcg gaggatctct attccttgca acattgaaga
      361 aattcaaatc agaagaataa aaggtatttt agcatgaaaa gaataaaaac gtttatcggt
      421 ataggagtag ctctattatc tctttctctt ctagttgcat gtggaacata aagttcaaag
      481 gatacttcaa caagtaatga tgagaagaca gtagcaacat ccaatagttc aaaagaaaca
      541 atcactttcg atacaccggt tgtaacagac gatgtgattg aatcaatacg cacttatgca
      601 gattatatag atctttataa aaatattttt gatgattatt ttactaaagc tgaggaaggt
      661 ttcaaaggca cagctatgga aaataatgac tcgtttacta aactaaaaga gtcaactcaa
      721 aaattattcg atgcgcagaa aaaaaggtta aataatgaag atagaataga aacaactaaa
      781 aacaatgtga ttgccaaaca ttgtcaaaca gtcctttcct ttttggtttt gactagcttt
      841 tttgtgaaaa attgtgtaaa atagaataga taaacgaggg gaaacctcgg aaaatttaaa
      901 ggagaatcca tctaatggta aaattggttt ttgctcgcca cggtgagtct gaatggaaca
      961 aagctaacct tttcactggt tgggctgatg ttgatttgtc tgaaaaaggt acacaacaag
     1021 cgattgacgc tggtaaattg atcaaagaag ctggtatcaa atttgaccaa gcttacactt
     1081 cagtattgaa acgtgctatc aaaacaacta acttggctct tgaagcttct gaccaattgt
     1141 gggttccagt tgaaaaatca tggcgcttga acgaacgtca ctacggtggt ttgactggta
     1201 aaaataaagc tgaagctgct gaacaatttg gtgatgagca agttcacatc tggcgtcgtt
     1261 catacgatgt attgcctcca aatatggacc gtgatgatga gcactcagct cacacagacc
     1321 gtcgttatgc ttcacttgac gactcagtaa tcccagatgc tgaaaacttg aaagtgactt
     1381 tggaacgtgc tcttccattc tgggaagata aaatcgctcc agctcttaaa gatggtaaaa
     1441 acgtattcgt aggagctcac ggtaactcaa tccgtgccct tgtaaaacac atcaaaggtt
     1501 tgtcagatga cgagatcatg gacgtggaaa tccctaactt cccaccattg gtattcgaat
     1561 tcgacgaaaa attgaacgtc gtttctgaat actaccttgg aaaataaaaa attgtaagtc
     1621 tagaattgat ttctaggctt tttatgttag tatggaagta tgataaggaa taaaaaacaa
     1681 gattatgtac tggcctacaa gcaaccagtt tcaaccactt acatgggttg ggaagaagaa
     1741 gctttaccga taggcaatgg ttctttagga gcaaaagtat ttggccttat aggggctgaa
     1801 cggattcaat ttaatgaaaa aagtctctgg tctggaggtc cacttcctga tagttcagat
     1861 tatcagggtg gaaatcttca ggatcagtat gtttttttag ctgagattcg gcaggctttg
     1921 gagaagagag attacaatct ggctaaggaa ctggctgagc agcacctaat tgggccaaaa
     1981 acgagtcaat atgggaccta tctgtctttt ggggatattc acattgagtt cagccagcaa
     2041 ggtacgactt tgtctcaggt gacggactat cagagacagc tgaatattag taaggcactt
     2101 gtgacgactt cttatgtcta taagggaacg cgatttgaac gtgaagcttt tgcgagtttt
     2161 ccagatgatc tcttggttca gcgttttact aaggaagggg cggaaactct agattttact
     2221 atagaactat ccttgacctg tgatttggct tctgatggaa agtatgagca ggaaaaatct
     2281 gattacaagg agtgtaagtt ggatattact gattctcata tcttgatgaa gggaagagtt
     2341 aaggataatg atctgcggtt tgctagttat ctagcttggg aaacggatgg agatattaga
     2401 gtttggtcag atagggttca gatatcagga gccagttatg ccaatctctt cttggccgct
     2461 aagacggatt ttgcccaaaa tcctgctagc aattatcgca agaaactaga tttagagcaa
     2521 caggtgatag acttggtgga cacagctaaa gaaaagggct atacccaatt gaaatcaagg
     2581 catatcgagg actaccaagc cttattccag cgtgttcaat tggatttgga agctaatgtt
     2641 gacgcattca ctacagatga tttgttaaaa aattataagc cacaagaagg gcaggctttg
     2701 gaggagctgt tcttccagta tggacggtat ttattgatta gttcgtccag agactgccca
     2761 gatgctctac cagctaacct acagggagtc tggaatgcgg tcgacaatcc tccttggaat
     2821 tcggactatc acttaaatgt caatctgcag atgaattatt ggccagccta tgttaccaat
     2881 ctcctagagg cggtctttcc ggtcatcaac tatgtagatg atttgcgtgt ctatggtcgt
     2941 ctagcggctg taaagtatgc aggaatcgtc tctcaaaaag gtgaggaaaa tggttggttg
     3001 gttcatactc aagcgactcc ctttggttgg acggcacctg gttgggatta ctattggggt
     3061 tggtcaccag ctgccaatgc gtggatgatg caaactgttt atgaagccta ttcattttat
     3121 agggaccaag actatctcag ggagaaaatt tatcccatgt tgagggaaac ggttcgtttt
     3181 tggaatgcct ttttacataa ggatcagcag gcgcagcgtt gggtgtcttc tccgtcttat
     3241 tccccagaac atgggccgat ttcgattggc aatacttatg accaatctct gatttggcag
     3301 ttatttcatg attttattca ggctgctcag gaattgggac tggatgagga cttgttgact
     3361 gaggttaagg agaagtctga tttactaaat cctttgcaaa tcactcaatc tggtcgaatc
     3421 agggagtggt atgaggagga agagcagtat tttcaaaatg agaaagtgga ggcccagcat
     3481 cggcacgctt cccatctagt gggactctat cctggcaatc tctttagcta caagggacaa
     3541 gagtatattg aagcggcacg tgctagcctc aatgatcgtg gagatggcgg cacaggctgg
     3601 tccaaggcta ataagatcaa tctctgggcg cgtttgggag atggcaatcg agcccataaa
     3661 ttattggcag agcagttaaa aacatccacc ttgccaaatc tttggtgtag ccatcctcct
     3721 tttcagatag atggtaattt tggtgctact agtggcatgg cagaaatgtt actccagtct
     3781 catgcagctt atctggtacc tctagctgcc ctacctgatg cttggtcaac aggttctgtt
     3841 tcaggcttaa tggcacgtgg acattttgaa gtgagcatga gctgggaaga taaaaaactc
     3901 ttacagttga ccattttatc aaggagtgga ggagatttgc gagtttctta tccagatatt
     3961 gagaagagtg tgattaaaat gaatcaagaa aaaataaaag cgaaatgcat ggggaaagat
     4021 tgtatttcgg tggcaacagc agaaggtgat cttgttcaat tttattttta agaagatgtt
     4081 ataaggcagt aatttgaaac ttccttttaa taaggattta agaatatagg cagtttccaa
     4141 ctagttgaaa aagcgttata atgataatag gaagtaatac tcaatgaaaa tcaaagagca
     4201 caaactagga agctagccgc aggttgctca aaacagtgtt ttgaggttgt agatggaagc
     4261 tgacgtggtt tgaagagaga ttttcgagga gtataatttg tttgatagag ggtgggtctg
     4321 atggcttata ttgagatgaa acactgttac aagcgttatc aggttgggga cacggagatt
     4381 gtggccaatc gtgatgtgaa ttttgagatt gaaaaggggg agctggttat tatccttggt
     4441 gcttcaggtg caggcaagtc aacagttctt aaccttcttg ggggaatgga taccaatgat
     4501 gaaggggaaa tctggattga tggtgttaat attgcggatt atagttccca ccagcgcacc
     4561 aattaccgta gaaatgatgt ggggtttgtt tttcagtttt ataatctagt ttctaatctg
     4621 acagctaagg aaaatgtgga actggcttct gaaattgtga cagatgcctt gaatcctgat
     4681 caggtcttga cagatgtagg tctggctcat cgtctcaata actttccagc ccagctttct
     4741 ggaggggagc aacagcgagt ctccattgca cgcgcggtag ccaaaaatcc taaaattctc
     4801 ctttgtgatg aaccgactgg agccttggat tatcagacgg gcaagcaggt tttgaaaatt
     4861 ctccaagaca tgtctcgtca aaagggagcg acggtgatca tcgtgactca taatggagct
     4921 ttggcgccca ttgctgatcg cgtgattcat atgcacgatg ccagtgtcaa ggatgtggtg
     4981 ctcaaccagc atcctcagga tattgacagt ttggagtact agcatgatca agcgaaaaac
     5041 ttattggaag gacttaattc agtccttcac agactccaag gggcgttttt tatccatctt
     5101 gatcctgatg atgttgggat ctctagcctt agtaggcctc aaagtaacca gtcccaacat
     5161 ggaggcgaca gctaatgctt atttaacaac tgctcaaacc ttggatttgg cagtcatgtc
     5221 taactatggc ttggatcaag cagaccaaga agaactaaaa cagacggagg gcgcagaggt
     5281 cgagtttggc tatttgacag atgtgactat ggataatggg caggatgcca ttcgactgta
     5341 ctccaaacca gagcgaattt caacctttca gctaagaaag ggacgacttc ctcagtcaga
     5401 cagggaaatc gctttggcca ctcatttgca aggccaatac agcgtgggac aggagattag
     5461 ttttaaagaa aaagaagagg gtcattcctc tttaaaagac catacttata ccattactgg
     5521 ttttgtggat tcggctgaaa tcctctccca gcgagatatg ggctacgcag gaagtggaag
     5581 tgggactctg acagcctatg gggtgatttt acctagtcaa tttgatcaga aagtctacaa
     5641 tatagctcgt ttgaaatatc aagatttagc aggtttaaat gccttttcat cagcttatga
     5701 agaaaaatcc aagcaacatc aggaagacct tgaacaaact ttatcagata atggcaaggt
     5761 acgtctgcaa cttttgaaaa aagaaggaca agagtctcta gacaaggggc aagagactct
     5821 tgacaaggct cagactaatt tgcaggaagg caagcgtcgt ttagcagctg ctcaagctcg
     5881 tatacaggct caagaaagtc aactagcctt gtttcctcaa gctcagagag agcaggctag
     5941 tgctcaactt acccaagcca agcaggaact gggcaaggaa gaggacaaac taaagcaagc
     6001 tgaacaaaat ctagcccaag aaaaggaaaa attagaaaaa catcagcaag tcttggatga
     6061 tttggcggag ccaaggtatc aggtttataa tcgtcagacc atgccaggtg gtcagggcta
     6121 tcttatgtat agcaatgctt catccagtat tcgagcagtg ggcaatatct ttcctgtggt
     6181 actttatgcc gtagcagcca tggtgacctt tacgaccatg actcgctttg tagacgaaga
     6241 gcgaactcat gcagggattt ttaaggcctt gggttatcgt agtaaggata ttatcgccaa
     6301 gtttctcctt tatggactag tagctgggac tgtcggaacg gctctaggta gtatacttgg
     6361 tcattatttg ctagccagtg taatttcaag tgtcattaca aaaggcatgg tggtgggaga
     6421 aacccagatt cagttctatt ggacctatag cttactagct cttgtcttga gctggttggc
     6481 gagtgtgtta ccagcctatc tggtggcttg gagggaactt catgacgaag cagcccagct
     6541 tctacttcct aaacctcctg tcaaaggagc taaaatctta ttggagcgta tcggttttat
     6601 ctggcgtcgt ctcagtttta ctcataaggt aacagcccgc aacatctttc gttataagca
     6661 gagaatgttg atgacaatct ttggtgtggc aggttctgta gctctgctct ttgcaggttt
     6721 gggaatccaa tcttctgtag caggagttcc gtctaaacag tttcaacaaa tccaacagta
     6781 tcagatgctt gtctctgaaa atcctagtgc gaccaatcag gacaaggtag agctagcaga
     6841 agtgttgaaa gggcaggaga tactagccta ccagaaaatc tattctaaaa cgctagacaa
     6901 ggatttcaag ggcagggctg gtcttcaaaa cattactctt atgatgatag agaaggaaga
     6961 tttgactccc tttatccatc ttcaacatca tcagcaggag ctgacattaa aagatggcat
     7021 cgttattaca gctaaactcg cccagctggc aggtgtcaag gttgggcaga ctttagaaat
     7081 tgaaggtaag gaactaaagg tcgctgctat tactgagaac tacgttggtc actttattta
     7141 tatgagtcag gctagctatg agcaacttta cggacagcta ccccaagcca acacttatct
     7201 ggtctcatta agggatacca gtgcaactag tatcgaaagt caggcgggct tgcttatgaa
     7261 tcaatctgcg gtgtccagtg ttgtccaaaa tgcttcagcc attcgactct tcgactctat
     7321 cgctagctca ctcaatcaga ccatgaccat cttggtcatc gtatcggttc tattagctat
     7381 tgtcatcctt tacaatctga ccaatatcaa cgtagctgag agaatccgtg aactctccac
     7441 tatcaaggtt ctcggttttc ataataatga agtcaccctc tacatttacc gtgagacgat
     7501 tgtgctgtcc cttgtgggaa tcgtacttgg tctggtagct ggtttctatt tacaccaatt
     7561 tttgattcaa atgatttcgc ctgcgactat tctcttttat ccgcaggtag gctgggaagt
     7621 ctatgtaatc ccagtggcag cagtaagcat cattttgacc ttgcttggtt tcttcgtcaa
     7681 ttattatctg agaaaggttg acatgttaga agccctgaaa tctgtagagt aaggtagtta
     7741 tttttagctg attgaacttc tatttactaa tattcaaaaa tcctccgttt caaagagcag
     7801 ggaactcttt gtgacagagg attttttcta tagggcttta gcagctgcaa ttgcggcttc
     7861 gaagtttggc tcagaattga tattatccac gtattcaacg tagcgaatcg tattgtcagt
     7921 atcgaggaca aagactgcgc gtgctaatag gtgccattcg ttgatcaaga gggcataatc
     7981 gcgtccgaaa gaatggtcaa agtagtctga aagcataatg gcattgtcaa ggccttcagc
     8041 accgcaccaa cgtttttgag caaaaggtag gtccattgaa acagtcaata cgaccgtgtt
     8101 gtccagtcca gccaattctt cattaaaacg acgtgtttga gttgagcaga tgcctgtatc
     8161 gatagaagga acgacactca agactttttt cttgccatca aaatcagcca gagatttttt
     8221 agaaagatct gttgtagtaa gagaaaaatc aagcgccttg tcgccgactt gtagttgttt
     8281 acctgtaaag ctcacaggat ttccgagaaa agttaccata ggatactcca atcttttttc
     8341 ttccattgta tctgaaacag tcagaatttt ccaatgattt gaccggaaat gtgtgcatag
     8401 aaaaaacgcc agctcacatg agaatgacgt ttttcagagg cttattttgc caatccttca
     8461 gcaatcttgt caaggttgta tttcatcatg ctgtagtagc tgtcgccttc tttaccttgt
     8521 tctgcgatag agtcagtaaa gatttgtgcg tagattggga tgtttgtgtc ttgagaaaca
     8581 gttttcattg gacggtcatc cacacttgat tctacaaaga gtgatggaac ttttgtttgg
     8641 cgaagttttt caaccaaggt cttgatttgt tcaggagttc cttcttcttc agtattgatt
     8701 tcccagatgt aggcacttgg gacaccatag gctttagaga agtatttgaa tgctccttcg
     8761 ctggttacaa tgagtttctt ttcagcaggg atcttattaa atttatcctt actttcttta
     8821 tcaagtttgt ctaacttatc agtatattct ttgagatttt tttcatagaa ttctttattg
     8881 ttagggtctt tggcgctcaa ttgtttggcg atatttttag caaaaataat accgttttca
     8941 aggttaagcc aagcgtgtgg gtcttctttt cctttttcat tttgaccttc aaggtagata
     9001 acatcaacgc cgtcgctgac tgcgaagtag tctttgtttt cagttttctt ggcattttct
     9061 accaattttg taaaccaagc attgccacct gtttcaaggt tgataccgtt atagaaaatc
     9121 aaatcagcct cagaagtttt cttaacgtct tcaggaagtg gttcgtattc gtgtgggtct
     9181 tgcccaatcg gaacgatact atgaaggtca attttgtcac cagcaatatt tttagtaata
     9241 tcagcgatga ttgagtttgt agcaacaact tttagttttt gaccagaagt tgtatctttt
     9301 tttccgctag cacatgctac aagaatgatt gcagaaagaa agagaacgag taatgtacct
     9361 aattttttca ttagatcctc caatttatta gggctttgcc ccttatttta acaaatgttt
     9421 atttttcagt ttcaaatatc gttgtttggg agcgataaag aagctaatga gaaagaaact
     9481 agcagctgta agcacgatac tagaacctgc cgcaacatta aaactatagc caataaagag
     9541 tcccaaaact gaagcagtag ctccgaaggt tgaggaaagg aaaatcatgc ttttcagact
     9601 attagcatac agataagcag ttgcagctgg ggtaatcagc atggctacaa tcaggatagt
     9661 tccgacactt tgcatggctg tcacagacac gagagtcagg agtaccatga gaaggtagtg
     9721 atagaaattg acaggcattc ccatggcttt agccaagagt tcatcaaagg aagttatcaa
     9781 gagttgcttg aagaaaatcc agattaacaa gaggatggct gcccccacac ccatagtaat
     9841 aaacatatcc gtatcttgga cggccagaat attaccaaaa aggatatgga aaaggtcagt
     9901 tgaactttta gcgacaccaa tcaagatgat accgagggct aagaaagaag aaaaggtaat
     9961 gccgatggcg gtatcgcttt tgataatcga gtttcctttg atgtaggtaa tgatgatggc
    10021 agctagcaat ccaaagacaa tggctccgat aaagaagtca aggcccaaga tgaaggagag
    10081 ggctacacct ggtaagacag catgtgaaat ggcatctccc atgagtgaca tcccgcgtag
    10141 aataatgaaa catcccacgg ctccagctac aatcccgacg acaatagctg ttatcaaggc
    10201 attttgtagg aaatggaatt tttgcaatcc atcgataaat tctgcaatca taggtcacct
    10261 ccattgaaaa agagttgatt accgtaagct tcttttagat tggtttcggt aaaagtttct
    10321 tttgttggac caaaggcaat cacttctcga ttgacaagta agacttgatc gaagtagtgg
    10381 ggaatcttgc tgaggtcgtg gtgaacgatg agaaccgtct tcccagcttt tttcaaatct
    10441 ctcagcgtat tcatgatgat ttcctcactg acagagtcaa tcccagcaaa gggttcatcc
    10501 aagaggatat agtcggcttc ctgcaccaaa catctggcaa tcaagacccg ctggaattga
    10561 cctccagaca gttgactaat ttgacgttca gcgtagtcag ctaggccgac gatttcaagg
    10621 gcctcttgca ctttcttcca atgtttagcc tttaaacttc gaaagagagg aatagaggga
    10681 aatagtccta acgagacgca ttccttgacc ttgatgggaa agttgtagtc gatattgatt
    10741 ttttgttcga cataggcaat tcggtgtaag gattttttaa cttccttgtc atcgagaaat
    10801 gcctgacctt gatgtgggat aattcccagc atacctttta atagtgttga tttcccagcg
    10861 ccgtttggac caatgatgcc ggtaattgtt ggtccatgga gcactagtga aatatcctta
    10921 agtgccaacg tttctttgta ggagacgctg aggttttcga tacgtatcat aaacttgtat
    10981 tcctcctgtc tcttaatata cattaaaaaa aaaattaagt caagttaatt ttt
//
LOCUS       DAVMGZ010000059        10917 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000059 DAVMGZ010000000
VERSION     DAVMGZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.060"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            114..278
                     /locus_tag="VYQ93_001242"
     CDS             114..278
                     /locus_tag="VYQ93_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV3335929.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            362..928
                     /gene="amaP"
                     /locus_tag="VYQ93_001243"
     CDS             362..928
                     /gene="amaP"
                     /locus_tag="VYQ93_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV3335930.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            940..1110
                     /locus_tag="VYQ93_001244"
     CDS             940..1110
                     /locus_tag="VYQ93_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV3335931.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1149..1757
                     /locus_tag="VYQ93_001245"
     CDS             1149..1757
                     /locus_tag="VYQ93_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV3335932.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1788..1991
                     /locus_tag="VYQ93_001246"
     CDS             1788..1991
                     /locus_tag="VYQ93_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV3335933.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2101..2379)
                     /locus_tag="VYQ93_001247"
                     /pseudo
     CDS             complement(2101..2379)
                     /locus_tag="VYQ93_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2577..2864
                     /locus_tag="VYQ93_001248"
     CDS             2577..2864
                     /locus_tag="VYQ93_001248"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV3335934.1"
                     /translation="MIVSLKTKSRKAKDIAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3142..3912)
                     /locus_tag="VYQ93_001249"
     CDS             complement(3142..3912)
                     /locus_tag="VYQ93_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV3335935.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3927..4775)
                     /locus_tag="VYQ93_001250"
     CDS             complement(3927..4775)
                     /locus_tag="VYQ93_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV3335936.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIANTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHSAGAPWWLQVPNVCWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4772..6247)
                     /locus_tag="VYQ93_001251"
     CDS             complement(4772..6247)
                     /locus_tag="VYQ93_001251"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV3335937.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6570..7925)
                     /locus_tag="VYQ93_001252"
     CDS             complement(6570..7925)
                     /locus_tag="VYQ93_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV3335938.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMAYKTEL
                     S"
     gene            complement(7935..8252)
                     /locus_tag="VYQ93_001253"
     CDS             complement(7935..8252)
                     /locus_tag="VYQ93_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV3335939.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8269..8586)
                     /locus_tag="VYQ93_001254"
     CDS             complement(8269..8586)
                     /locus_tag="VYQ93_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3335940.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLPGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8601..9497)
                     /locus_tag="VYQ93_001255"
     CDS             complement(8601..9497)
                     /locus_tag="VYQ93_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV3335941.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9500..10762)
                     /locus_tag="VYQ93_001256"
     CDS             complement(9500..10762)
                     /locus_tag="VYQ93_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV3335942.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
BASE COUNT         3755 a         2191 c         1682 g         3289 t
ORIGIN      
        1 aaattgattt tttgatatta aaataaaaaa ggagggtagg cagtgttgtg atcaattatt
       61 gctggaggtc ttattggtct cttggcaggt aaaatcacta aaaaagtagt tctataggaa
      121 tcatcgcaaa tgtattcgct ggtttagtcg gggcatatgc aggacaatct cttttaggta
      181 gttggggtcc atcaatcgct ggaatggctt tgctcccatc tattgtaggt gcagcgattg
      241 tgattactgt agtgtcattc tttacaggta gaaagtaaac ttttcgccag taaagttagc
      301 aaactatttt taaatcaatg acgggaaaaa tagtttaaat gttaaatcga aaggattgta
      361 tatgtcaaaa gcaaagaaaa tatgtttcat tattttctgt attttaatct tgacaatttt
      421 ccttcctgtt ttgatagatt atcatcaagt tagtgatcta ggtattcatc tacttagctg
      481 gagacagaac tccgtagttg aattctatct tgctagatat gtcttttggg ggacagtggt
      541 tctatcaact ttagttttat tatccatttt agttgtgatg ttttatccta aacgttactt
      601 ggaaatccaa cttgaaacta aaaacgatac attaaaatta aagaattcgg caatcgaagg
      661 ttttgttaga agtttggtga gtgatcatag attgatcaag aacccaactg ttcatgtaaa
      721 tttacgaaaa aataaatgtt tcgttcatgt agaaggtaaa attcttcctt cagacaacat
      781 cgctgacaga tgccaaataa ttcaaaatga aataactaat ggattgaagc agttttttgg
      841 tattgagcgt caagtaaaac ttgaagttgc agtaaaaaat taccaaccaa aacctcaaaa
      901 caaaaagact gttagtcgtg tgaagtaagg aagtaaaaaa tggaatggct taaacaatat
      961 cgatatccaa ttatcgctgg tctcataggc gtatttctgg cttgtttgat tgtctccttt
     1021 ggcttcttca aaacaatatt tgtattgatt ttaggagcac tgggagttgc agctggatta
     1081 tatatcgaaa aaaactatat agataaataa aaaaataaaa attactaatt taattaaagg
     1141 agtttcatat gtcaaacgaa aaaaacacaa acactaacgt agaaaagaaa gatgctactg
     1201 ttgtagctca cgaaatcaaa ggggaactta cttacgaaga taaagttatc caaaaaatca
     1261 ttggtctttc actagaaaac gtttcaggtc ttttgggaat cgatggtggt ttcttctcaa
     1321 atcttaaaga aaaaatcgtt aacagcgatg acgtaacaag tggtgttaac gtagaagttg
     1381 gtaaaacaca agttgcagtt gacttaaacg ttattgttga gtaccaaaaa aatgttccag
     1441 ctttatattc agaaatcaga gaaatcgtat cttcagaagt tgctaaaatg actgacttgg
     1501 aaattgttga aatcaacgta aacgttgtcg acatcaaaac taaagaacag catgaagcag
     1561 actcagtaag ccttcaagat cgcgtatctg acgttgctga atcaacagga gaattcactt
     1621 cagaacaatt cgaaaaagct aaatctggtc ttggatctgg tttctcaact gttcaagaaa
     1681 aagttagcga aggtgtagaa gctgttaaag gtgcagcaaa tggtgtagta tctcacgaaa
     1741 acactcgtgt aaactaagat aaaataaata taacaggaga aattatcatg tcagtagaag
     1801 aaaaattaaa tcaagctaaa ggttctatta aagaaggtgt tgggaaagcc atcggtgatg
     1861 aaaaaatgga aaaagaagga gcagctgaaa aagttgtttc taaagtaaaa gaagttgccg
     1921 aagacgctaa agacgctgta gaaggtgctg tagaaggtgt taaaaacatg ttgagtggcg
     1981 acgataaata aggttaaaag ttactttatc tttttggtaa cattagtcaa aagagtctga
     2041 gtcaagatga ttctcagaaa acaaaaagct agagattccc aattgcggaa ctctagcttt
     2101 ttaattttgc ctctttctct tattatattt cagcaggttg ttggccatga gtatgaatcc
     2161 catgtcaatt ctcacttgac gcttacctct cagatgacat ctcttataac ccaaacaaac
     2221 ctttatctgc ccaaagacag atttcatatc aatcttacgt ttagcgaaaa tttgtctacc
     2281 cttggaagat aaaagtgcct gatattcttt agtttttaaa cactggtaac gttcattcat
     2341 atacagtccc ttttgagggg ctgattcagg ttcataatcg cagtcaacat tgatttcaag
     2401 gctgtttgct ttctatctcc cctttttcat gatatatttt cgtacaggtg cttcaatagt
     2461 ttctatagta aaatgaacca aaaatagtac acgttgcggt ataatgttct tgtggcatat
     2521 tcattagatt ttcgtaaaaa gttctctcct atttgtgagc gaacaggtat agtcaattga
     2581 ttgtcagttt gaagaccaag agtcgtaagg caaaggatat agcagaatcc atccaagggt
     2641 ggctggcaca atttctagtg aacctcttta aatctattac gtttgactgt ggaaaagagt
     2701 tttcaaaatg gaaagatatt tccaatcatc acgatagtga gagctttttt gcgaatctag
     2761 gatgtccaag acagcggtgt ctcaacgaac attcaaatag acttttaagg tgtcatgact
     2821 tgccaaagca gacagatttc aacgaagtca gtcaagaatt ttaggctggg taaaaactca
     2881 atttcaggag aaaatgaaat aaatattccc acaataaaat gcatagtatc aaagtttttc
     2941 caacacctga tattatgcat tttttaattt taaagacttt ttcccagcct caagaaatca
     3001 catcccacct aaatcctttg gataccaaat acctcatcag gtattttctg agttggtaag
     3061 actgtctagc ttaatttgac aaatggaggg cgttaaatcc agtttttaca ggaaatctat
     3121 ttaaatattt tcacactgac cttacaaaat atgaaataaa ttatataaag tcattcccat
     3181 gacaaagaat aacatccctt taaaacagta tcttacccaa ttttcaggta agacttttga
     3241 tacaaccgtc cccaataccc ctccgcatat agaagcaaga aaaataactg gaacaggtgc
     3301 taaatgatac tgatttaaac ctgtaaccac aacaattgta gccaaatgag acagctggga
     3361 aaagaaaata attgcaagag aatacatcgt tgcttctttt atcgaaatag aaaaaaacac
     3421 catcaataaa gatacattga gcggaccacc accaatccct aaaaaactag aaatcaagcc
     3481 caatagaaga ccacaaatca tatagaatcc tatcttatca aattgataag tttttttaaa
     3541 atctgttaaa acaaatgata caagtagcat cacaaatagt aaaatcattt gaacaatgga
     3601 tactagccgt actgataatt gagataacaa aacttgaaaa ataagatgac ctagaaatcc
     3661 tcctaaaaca gaagaaaagg ataccgtaaa aatcaatctc cattttatct ctttactttg
     3721 tgaaaatctt ttgattgttg aaataatcgc cattataaaa acagagaaac tagaataaaa
     3781 agcaatatca ctgaccgaat ggtacccaaa actatccata agtggcttaa ttataattcc
     3841 accacccatg cctgatatag aacccaatcc actagctatt ataatgatta gaaaataaat
     3901 aatctcaacc actttttaag tccatcctat tctttcaaac atctaaaacc tagatggcta
     3961 ctagtggaag tagaaataca tccatttcta gcagctactc ggtatcgatt acaataagaa
     4021 caatgacata aaaaggaacc tcctctgata gcatactcct catcttgtat cccatatttt
     4081 ggcaactcat agtcatcttc attaaaacta gctaataagg tatatctcgg attgcgacac
     4141 cattcccaaa cattcccaat catttgatat agaccaaaat cattaggtaa aaattcatag
     4201 acaggagccg ttcctataaa tccatctaag gcagtatttt cataaggaaa tcttccttgc
     4261 caagtattgg catgataata tccaccttct aaaagagtat ctccccaagg atattcagat
     4321 tgtcttcctc ctctagcagc atattcccac tgtgcttcag tcggtaaaga cattccgctc
     4381 caattacaga aagccaatgc atcttctaaa gcaacatgaa ctactggatg gtcttctaaa
     4441 cccactagat tactattctc accgtaggga tgtttccagc aaacgttcgg aacttgtaac
     4501 caccacggag ccccagcaga atgaggatat ccctcccttt ctgcctctgg aacgaacaat
     4561 ataaaaacaa aagaccactc ttgtcgctca gctagtgttt tatagcctgt ttctttcaca
     4621 aattgagcaa attcttgatt cgtaactgga gtattcgcaa tggaaaaatc ctttacacta
     4681 acagcgactt gaggaccttc aaaatcatct aaaaagccct cttcagaatt tgttcccatc
     4741 ataaatgtcc cacccttgat ttttttcatt ttcatgattg actatccccc gttttcgtta
     4801 gagttggcgt tattttagaa agttcaacag gaactaactc ttcatccaca acaaaacctt
     4861 cttctctata tctaagaaag tctactaatt ttttcttcat ctgacgaaca ataggttggt
     4921 atttttcact tggatacaaa tcattcattt catgcggatc ttttttcata tcaaataact
     4981 gataatgatt taaaacagga aaccagataa atttccactg gtctgtcaag atatattgac
     5041 tagaatcttt tcctaaagca tgttcaccat gaaattctgt tcgccacccc tcgtactgtc
     5101 caaataataa attcttaaca ctccgtccat ctagttcatc tgtagtagtt cctcctgcca
     5161 aatccacgag tgaaggaaaa atatcttgaa ttttcactaa ttgcttgatt gttcccctat
     5221 taccagctat caatccagcc ggatcgtaaa taaacgaagg aatgtggata cttccctgat
     5281 aaggatatcc ctttctaaac agataatgtt caccaagctg atctccatga tccgaaacaa
     5341 accagataat cgtatcttta tcatgacgaa attcttttag ggcagtcaaa aagcgactaa
     5401 tttgatggtc tatatgagta atcaatccaa aataagcagc aaccattctt ctttgatcat
     5461 cttcctttaa tttccctctt aaagcataaa tagacggaat ttgcttttct aatacttccc
     5521 aattcccgat atgcaaatcg agaaattgag gcaagcgttc catatacata tcaaaatagt
     5581 attttggggg atttaatgga gcatgtggtt tttcaaaaga cattttgaga aaaaaaggaa
     5641 ccgttggatc tcttctttgt aaaaatgaaa tggactcact cacaacccag tttgttggat
     5701 gtaacttctc atctttatcc caaggtctag cttcccaaga attacaatcc attccgtcat
     5761 caatcaaatc aacatcataa ccaactttac cctttaaaaa ggctagatag tcactagcat
     5821 aatcaaattg acttccataa gccttatcat actttctatc aacatgtaga tacccatcgt
     5881 gaagtaagac atgatcaaac ccaagtctct gtctggaagg aaaaacatgc atcttaccaa
     5941 tacactctgt ttgataaccc atatctttaa agacttttgg aagagtatta gtaaaattcc
     6001 aaggcacctc atcttgataa ccaactcgtc cacttttatc ttggtctaag ccagtcaata
     6061 aagctgccct agcaggaacg caagaaggaa ctggagaata ggcattttca aaattataac
     6121 ctacactagc catcatatct aatgttggag tgctaactag cttatcctta ctattcaagg
     6181 ataaggcgtc tgctctcatt tgatctacaa caatcaaaat aatatttggc tgttttgtct
     6241 gaaccataaa atctcctttc taatatggca aaagaggcac aagaagatat ctacctttac
     6301 tgcacccctt tctatatcaa tctctctata taaaacaata acattcttgt tatgttttat
     6361 agaaaaatga actaaaatat gactaaatcg attaggaaat tcaaatcatt ttctagtact
     6421 gttttagaag ttacagtgta ctattccaac ttcaataaac tataaacctt tgtctaataa
     6481 caattttagt ggagataaga aatcctacac ctaactcatc ttacacgtaa tctatttcta
     6541 ttttatcaca aaaaacgcaa gtaagaccat taactcaatt cagttttata tgccattttc
     6601 acaaatggga aataagtcaa gacactaata atcaaacaaa caactgataa gatgatggca
     6661 cgccaatcaa atgctgtaga gaagaaacca tataaaattg gaggcattac ccaagtaacg
     6721 ttttgtgtaa caggtgaaac aagaccccag cttgttgccc agtaagctac cgttgccatg
     6781 aaaaccggac taagtacaaa tggtataaat agcaaaggat tcaagacaac tggtaaacca
     6841 taattgaccg gctcaccaat attaaacaga actggtgcta gaccaagttt agcaactttt
     6901 cgataatgac tgtttcttga aaaaattaaa atagcaagta ctaatcctaa tcctccaaac
     6961 cagacaaacg ccccaaaaga cccacttgtc catatataag gaatcggttc acctttttgg
     7021 aaagcatcca gattcgctaa catagcaact ccaaacagcc cttccatgat gggagccaat
     7081 acatttcctc catggagacc aaaaaaccag aataacttat tcaaaaagat catcagaata
     7141 actgcaaaga aactttgaga caaacctagt aatggcgttt gtaacacctt gtaaacccaa
     7201 tcaatcaata agtcattgct aagtaaatgg aaaacataag tcaagatggc tactatatac
     7261 atcgccataa atcctggaat gatagaagtg aacggcttag caatcgcagg gggaactgaa
     7321 tctggtaact tgattaccca gttctttttc attactttac agaaaataat agaggctaaa
     7381 aatccaatca tcatggctgt aaagtagcct ctggcattaa tatggtttcc tggaatcaca
     7441 ttcccaatag ttaccatcag atttttacca tcaaatgcta gattatcaat tccttgtaag
     7501 atttgatcta atttcacatc tcctacattt gccaaaggga aactctttgt aactgtactt
     7561 ccaatcgaaa tgacaaacga agcaagtgat accaaaccag cagaaactgt atcaaccttg
     7621 taaatcttag cgatattcac tcccaagcaa tagatgaaca acaaggaaac aattggtata
     7681 cttcccttga ataccaaatt attgatgtca acaagccact gaaaggtttt cgtaatactt
     7741 tctaggtgaa attgttgtgg taaatccact agaaaagcat ttaataacaa agcaatggaa
     7801 cctgtcataa taacaggcat agtccccaca aatgaatcac gtaaagcaac taaaaacttc
     7861 tgattcccaa ctttcattgc aattggagaa agtttttctt gcattttatc caacattgta
     7921 ttcataccaa actcctaatt tttttcaaat cgttcgtata cttctaccac ttctttcgcc
     7981 aagtcaacaa aagtcagact cgtcattaaa tgatcttgcg cgtgtaccat caagaggcta
     8041 attgatgttt tttccccctg tgcctctctt gtcaacattt ctgtttgagc cttatgcgcc
     8101 tgtaataagg cagcattcgc atcagctaat cttcgattgg ctttagagaa atcccctttt
     8161 tttgctgcct gaatagcctc catagcatta cttttggctt ccccaccata cataattagc
     8221 cccattacag attctaaatt actttcatcc ataacattct cctcaatctt agtctaataa
     8281 actttcagct aaatctaaaa ctttagaacc attcatcatt ccgtaatctg tcatcggaat
     8341 aaccgccaca ggaatctgtc tatcttttag tttttcttga aagtcccctg gtaaatagcg
     8401 aacttgagga cctaaaagca acacatttac ttcttttgtt gctacaattt cttctgcttc
     8461 aggagctgga actgcaaaaa tttctgcatc caacccctta tcttctgctg ccttttgcat
     8521 ctttgtcact agcagacttg tactcatacc tgctgcacat gctaacataa ttgttacttt
     8581 agccatagct tactccttta tcacctattt actgacctag ccaagcgaga tactgtatgt
     8641 ctcactcctc taattgcctt gctcaatgca taaatattat cgactgttgc cagtccccca
     8701 tatcctgcgt cacctatatg ttgaatatca actccacaaa ttttatttct aagtgcaatt
     8761 tccttgatag tatctgtatc agatgtttct tggctagtac caatagcact caatacaaga
     8821 ccacccttac tatgaacgag atcaacgact tcacgcaact cttggtcatg aaaggctgga
     8881 acggttccaa ctgctggaac aagtatcaca tccgcacctg cttccaacaa ttgctctgcg
     8941 acagaaagct ctgccacagg ctcattcact cctgccccgt gcattttacc agctataatc
     9001 aaacctgaga aattctcttt agcagattga actgctttga taatctcttg attgctaact
     9061 cctgttccag gatttccagt caaacagaca aagtcaaaac ctaactcctc tattcgattc
     9121 aatgtttcaa cactagcaac acgacctgca acaatttcct gtgtttcctc tagcatcttt
     9181 gcagatgggt caatcggttc caaatttaca ccaattggac aagcaacaag ctggtgtaag
     9241 cgatgaataa catcatcacc tgaactatcc aaagcataaa ttttaggctc aaaaacatca
     9301 acacaattca ataaaatcat atcagctcca aaagctctag caatttcaga attagttata
     9361 tccccaacaa atgtctcacg agtaactaca ttttcagata gaacaactct accttcactt
     9421 gctaaaatac tttgttttaa ttcttcagca ttcatctgta atatctcaga tggatttgca
     9481 ctaattaaac gtttcattct caatcctcca ataacttaaa ccgtttttca atagctatta
     9541 caaatggtaa ataaagtaag acatccaaaa caatcaatac aatttgtaat aatgaaccag
     9601 aaatacttcc tgtagcaagt aaaccactta taacaggtgg catagtccaa cctggatctg
     9661 tataagttag cggaaccaaa cctgatgcca ttgctcccca cgctactagt aagttaaaca
     9721 ttggagctaa gataaatgga actagtaaca aaatgttcaa aacaatagga acgccaaaca
     9781 ttgctggttc atttatatta aataatccag gtataacagt tataggtgcc aaagttttta
     9841 gttgtttact agctttcttc ttatgtgcta aatatcctaa cgcaagaact aaacctatgg
     9901 ttgcaccacc gccacctatg aatacaaaat tatccataaa tacgctagta aaaatatttg
     9961 gaagagtttc tccagtttga taagctactt tatttgcatc tagattagct aaccataacg
    10021 gtttcataac tgcattaaca acatttcctc cgtgtaatcc tacaaaccaa aaactactat
    10081 taaggaaaat taagataaga aggccaataa cattatttcc taaaagtcca agtggacctc
    10141 ccaaagctac ttgagcaatt tcatgaagat taggtaaact taatttatct aaagttgcat
    10201 aaacaatcaa ccaaccaaca atagtcactg caccaggaat aatggcacta aagcttttag
    10261 atactgctgg tggaacacca tccggcattt ttatctgtat attatgattt ataaaccatt
    10321 ggtagatata gccattaatc aatcctaaaa tcatagcaac aaataaacct tttgatgcca
    10381 tgaaagcggt aggcattcct gctccagcct ctcctctaat gaaaggcgta actgtaataa
    10441 aagatgataa tgacacaata ccagcaggaa taccatcaac tttgtattgt ctagccataa
    10501 agtaagcgat accaaaacta gctacaagac caagtaatcc aaaactacta tcaacaccct
    10561 tccataaata gtcagcaaca ccaatatcac ccagatattt ttcccatcct ggaataggaa
    10621 aacttgcaat aaccattagt aatgaaccta tcaacaataa aggtatagtt agagtaatac
    10681 cgtctcgaat agaaaccaac gctttattat tacccaatct agccgctact ggaagtaact
    10741 tttcttgtaa taatttttcc ataacctttt cctcttaaat atattttatt atcaaaagtt
    10801 atcaattatc tttatttttt gacgtcaagt tgttatttga ttatcttttg ttatttatat
    10861 tttacaacta taagaaagcc ttgtcaagga tttgtgtaaa catttgatta tttttta
//
LOCUS       DAVMGZ010000060        10641 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000060 DAVMGZ010000000
VERSION     DAVMGZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.061"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            55..336
                     /locus_tag="VYQ93_001257"
     CDS             55..336
                     /locus_tag="VYQ93_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV3335943.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            468..1412
                     /gene="manA"
                     /locus_tag="VYQ93_001258"
     CDS             468..1412
                     /gene="manA"
                     /locus_tag="VYQ93_001258"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV3335944.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1434..2909)
                     /locus_tag="VYQ93_001259"
     CDS             complement(1434..2909)
                     /locus_tag="VYQ93_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEV3335945.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMNFLVPNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKGLE"
     gene            3075..3239
                     /locus_tag="VYQ93_001260"
     CDS             3075..3239
                     /locus_tag="VYQ93_001260"
                     /inference="COORDINATES: protein motif:HMM:NF024803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV3335946.1"
                     /translation="MVPLKSENGYRTYSQEDLERLQVILYYKYLGFSLEKIAELLKEE
                     RTDLLLHLTR"
     gene            3240..3746
                     /locus_tag="VYQ93_001261"
     CDS             3240..3746
                     /locus_tag="VYQ93_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HEV3335947.1"
                     /translation="MDYLTRERQHLDTLISTLQKTIQEQKGERKMTIEEKFTGFSYQD
                     NQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAFNQVFQTLAQNLQVGLPATATENQ
                     EQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEFKENIDKFGSETAQYTSDAIAAYV
                     QTNAEKIG"
     gene            complement(3766..4221)
                     /locus_tag="VYQ93_001262"
     CDS             complement(3766..4221)
                     /locus_tag="VYQ93_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV3335948.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4241..5416)
                     /locus_tag="VYQ93_001263"
     CDS             complement(4241..5416)
                     /locus_tag="VYQ93_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV3335949.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5475..6320)
                     /locus_tag="VYQ93_001264"
     CDS             complement(5475..6320)
                     /locus_tag="VYQ93_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV3335950.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6445..7002
                     /locus_tag="VYQ93_001265"
     CDS             6445..7002
                     /locus_tag="VYQ93_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV3335951.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7021..7488
                     /locus_tag="VYQ93_001266"
     CDS             7021..7488
                     /locus_tag="VYQ93_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV3335952.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7577..8206
                     /gene="upp"
                     /locus_tag="VYQ93_001267"
     CDS             7577..8206
                     /gene="upp"
                     /locus_tag="VYQ93_001267"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV3335953.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8380..8970
                     /gene="clpP"
                     /locus_tag="VYQ93_001268"
     CDS             8380..8970
                     /gene="clpP"
                     /locus_tag="VYQ93_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV3335954.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9049..9297
                     /locus_tag="VYQ93_001269"
     CDS             9049..9297
                     /locus_tag="VYQ93_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV3335955.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9398..10558
                     /locus_tag="VYQ93_001270"
     CDS             9398..10558
                     /locus_tag="VYQ93_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3335956.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
BASE COUNT         3365 a         2106 c         2276 g         2894 t
ORIGIN      
        1 tataatgctt tttgatttta gtatagtatt atcgtagaaa atggactcgg tgctatggat
       61 acaccggatg agaatggtta tgtagcagat gactatcgga ttacttactt agaggcccac
      121 atcaaggcca tgcgagatgc catttacaaa gacggggttg acttgcttgg ttatacgaca
      181 tggggctgta tcgattcggt ttcagctggc acaggggaaa tgaataagcg ctatggcttt
      241 atctatgtag atcgagataa tgtaggaaat gggactctca aacgtagcaa gaagaagtcc
      301 ttctattggt acatgtcatt tatagcaatg gtgtaagcat cgagtagagc acatttgttt
      361 actattttta taaaatcttc tccctgcttt ataagatgta aaaaagagtt caattcgaac
      421 tctttttttg ctataatgag gggagaaaaa tcagacagga gattaagatg tcagaaccat
      481 tatttttaca atcagttatg caagaaaaaa tctggggtgg taccaagcta cgtgatgagt
      541 ttggctacga catcccaagt gaaaaagtcg gagagtactg ggctatctca gctcatctaa
      601 atggtgtttc taaagttgcc aatggtcgtt acgagggaac agatcttgct actttgtatg
      661 cggaacaccg tgaattattt ggcaatcgtc ctgagcctgt atttccgctt ttgactaaga
      721 ttttggatgc taacgactgg ctcagcgttc aagttcaccc agacgatgct tatggactag
      781 agcatgaagg cgaacttgga aaaacagaat gctggtacat catcgctgcg gatgaaggtt
      841 cagagattat ctacggccac aatgccaagt caaaagaaga actccgccag caaatcgagg
      901 ataagaactg ggatgcgctg ttgacaaaag tgccagttaa agcaggagat ttcttctatg
      961 taccaagcgg taccatgcac gctatcgggg cgggtatctt gattcttgaa acccagcagt
     1021 ctagcgatac cacttaccgt gtctatgact ttgaccgtaa ggatgacaag ggcaatttac
     1081 gtgaacttca ccttgaaaaa tctatcgatg tcttgaacat tggtgaaccc gcaaatagcc
     1141 gtccagtgac aatcaaagca gatgatctac gttccactct ccttgtatcc aatgatttct
     1201 tcgcagttta caaatgggaa attactggaa aagttaactt tgaaaagaca gctgactaca
     1261 gcttgttgag tgtcttggct ggtcaaggtc aactaactgt tgacgggaaa aactatccaa
     1321 tccaaaaagg gagacacttt atcctaccaa gtgatgttga agcttggact ctggaagggc
     1381 aagatttaga attaattgtt agtcatccat aaaaaagaaa gggcctgagt tcattactca
     1441 agtccttttt gattacataa attgggcgat gaagaccacg atgatgattg gaatgacgga
     1501 acgaagaaac gaagaaggaa gagccagact tggaacagtc cttgtttcca tgctctttca
     1561 tcgagatgga gttcctccat tgcaagagcc tttttaaaga tatagcctgt aaaaagtgaa
     1621 aggtagagag ctccaaatgg catgaggaga ttgggaacca agaagttcat agcgtcaaag
     1681 aaggtcttac caaaaatgtg aacatccgcc atgacaccgt aagatagggc tgaaggaatg
     1741 ccaaagacaa aggtcaaaat tcctaaaata acactccatt tggcacgttt gctgttatcc
     1801 tggttggtga tattgcccac attgatctcc agcatcacga cagaagaagt gacagtcgca
     1861 aagaggaaga ggacgtagaa aatggttcca aaaggcatct tgtcaaagag ttgaggcaag
     1921 acgataaaga gcaagctcgg tcccccttca gactggatat tgaaggggga ccgagcttgg
     1981 aaaatggcta gacctgccat gatggatatc gagatattca tggctacgat ggagattcct
     2041 gactggacta gattggtttt cttgtccaag taagaagcat aggtcaacat gactgtaacc
     2101 cctagtgaga gggcaaagaa agattgtccc agagcataga ggagaccagc gctagtcagt
     2161 tttgaaaagt ctggtttgag gaagtaaaga accccttcca tggcatttgg caaactgaga
     2221 gagcgaccga tgataacaac aaagatgata aagagcaggg gcatcatgac tttcgaagct
     2281 ctttcaatcc ctttttgaac cccacgtgat acaataaaga tatttaataa gataaaggct
     2341 gcttgagctc ctagggcaat ggctggattt gaaatgattg aagtaaataa ctgagcataa
     2401 tcacccgttc caccaagttg gaacaatttc ccaaactcaa tacctagata gactagaatc
     2461 catcctccga taacactgta aaaagataag aggataaaga gggcaaaggc gccaatccaa
     2521 ccgataaagt tgtacttgtt attcttgccc agttttccaa aggttttgat agcggaaacg
     2581 ccagcactac ggccaagggc aaactcagcc agcaggagag ggaaaccgat taaaatagtg
     2641 gaaatgagaa agattagtaa aaagcctcca ccgccattag cagcagtcat gtagggaaac
     2701 ttccaaacgg aaccaagccc gatgaccgag ccagcagatg ctagaataaa gccaagtttc
     2761 gacccccatt gtgatttttc tgacatagtt aaactctgat ctcctttttc aaaataagaa
     2821 aaagctactt gagttgtcaa atagccttct gtttgattga tagacttgac tatcctatta
     2881 tgttttctaa ggtctgtcaa gaaaaccatt ttcaagtttt cacacctttc tcaaaaacgc
     2941 ttgactctga cctaagggga ggggttatac tatcattgta aggaggaaat catgtaccat
     3001 ataaaagaag ctgcgcagct ttcaggtgtc tctgtcaaga ccctgccctg catcactatg
     3061 acaagatagg actcttggtc cccttaaagt cggaaaacgg ctatcgaacc tatagtcaag
     3121 aggatttgga acgccttcag gtcattcttt actacaaata tctaggcttt tctttagaga
     3181 aaatagcaga actgttaaag gaagaaagga cagatttatt gctccatttg actaggtagt
     3241 tggactatct aactcgcgaa aggcaacatc tggatacctt gatttccacc ttgcaaaaaa
     3301 ctattcaaga acaaaaagga gaaagaaaaa tgaccattga ggaaaaattc acgggattta
     3361 gctatcaaga caatcaaaaa taccaccaag aagcggtaga gaaatatggt caagaagtca
     3421 tgggacaagc gctcgaacgc caaaaaggtc acgaagacga ggctacggcc gcctttaacc
     3481 aagtctttca aactttggca caaaatcttc aagttggttt acctgcaaca gcaaccgaaa
     3541 accaggagca agcagccaag ctcttgcaag ccattcgcac ttatggattt gactgctcta
     3601 ttgaggtatt cggtcatatc ggtaaaggtt acgtctacaa cccagagttt aaggaaaaca
     3661 ttgacaagtt tggttctgaa acagcccagt acacgtcaga tgccattgcg gcttacgttc
     3721 agacaaatgc agaaaaaata ggctaggaat ttcctagcct attttttact tcaaatcata
     3781 aagccagtcg tcaccgtttt tgtagtaaaa gaattcactg agatcttctt ctagaaacac
     3841 acgaagcata tcagacatat catcggttgc aagttttaga tgagaaagat tttcaaagtc
     3901 ctcccaccaa actttccctt cgtctgaaga ctggagttca ccagtaaagt gttctgtctt
     3961 gtaaaaaagg acgacataac gataatcctt gtcgtcatac cagtttttga taccacagag
     4021 ttggggtttg gaaatgatca gaccagtttc ttctttcact tcacgaatga cagcatcgac
     4081 aaaggattcg ccacgttcaa catgaccacc aggaaaagta atgccagacc agtcgggact
     4141 aactcggtct tggaccagga ccttatctcc gtttttaatc atacacatgt taacaaattc
     4201 gactgcctct cttctgttca ttcttcacaa cctttaatct ttaatcataa tgcagacttc
     4261 ccgccaccca gccggtacag agggcagaag tgatgttaaa gccacccgtg tgggcattga
     4321 tatccataac ttcgcctgca aagtggaggc caggtaccag cttactttca agggttttag
     4381 gattgatttc cttgagactg actccaccct tggtaacaaa ggactttgca agggacattt
     4441 ttccagttac aggaatttta agttctttaa tggactggac aagttgttct cgttcctttt
     4501 cagtcagttg tttgactttt tcaggatatc cttgtacaaa aaattcggcc aagcgttctg
     4561 gtaacaaggt ttttaaagcg tttttcaagg atttttcccg attttcttct agaaatgtaa
     4621 ccaagtcctt ctcagaaagt tgaggcaaaa catcgagtga gagaacctcc ccacctttga
     4681 caaagctaga catgcgtagg gcagcaggac ctgacaaacc aaagtgggta aagagtaaat
     4741 catgagtgat gacatgctta ccataactta gggtcacatc gtccagagaa atcccttgta
     4801 aggctttatg tggaaaatct gttaataaag gactttcagc agcctcaaga tcggtgatgg
     4861 tatgcttaaa atggcgagca atctcgtgac caaaaccagt cgaaccagtc gaaggataag
     4921 acttaccacc tgttgtgaca atgagtttct cacaagtgaa ggtttgatcc gctgacttaa
     4981 ggacaaactg gtcatctact tttttaacag aaacgatttc tatttgagta gcaacttgac
     5041 cacctagttc ggtgattttc ttttccaaag cttcgataat agtccgagac ttgtcactgg
     5101 ctggaaagac gcgtccgtgg tcttcgacct taagtttaac accattttct gtaaaaaagt
     5161 tgatgatgtc atgattatcg aactgggaga aaacactgta aagaaagcgt ccgtttccag
     5221 gaattccagc tagcaggttg tctaagctac cattgttggt cacattgcaa cgtcccccac
     5281 cagtcccagc taattttttt ccaagtttcc gatttttttc gatgaggagg gttttctgtc
     5341 cataaaagct actggaaatc gtagccatca taccagcagg tcccccaccg atgacaatag
     5401 tatcaaaatg tttcatagct ctattgtacc acaaaaaaac aagagatgat ggtcacctct
     5461 tgtcaagaat gcaattaatc aatttcatag cccatcagca aaccgccctc ttctgcatag
     5521 aaactgcaga gaccagaggt tggtagaatt ttaatatccg cttgtgggaa ggtttcacgg
     5581 attcgctctg agagctgttg acaacatttt tcgttattgc gttgggccat gacaatacgg
     5641 ccaccagcat atccagcttt tactaactca tcataggcag cttgaactga tttctttgat
     5701 ccccttgctt tttgtagcaa ttcgagagtt ccagtttcac tagcttttcc gaccatacga
     5761 atgttgagaa ggccaacgac cgtaccgata agcttgctca aacggccgtt cttcaccaag
     5821 ttatcgactt tggctaggac aaagagcaac ttagtttttt cttgataggc agtgatagct
     5881 tcaaccactt cttcaaaaga caagccctgg tcaatcaagt cattcaattt ttctacgagt
     5941 aggtcaactt caccaccagc agataaacta tcaatcacat gaatcttagt gtcaggatgg
     6001 tcttctagat aaatattctt tgctagttga gcactattgt gactgccaga aagggtacct
     6061 gtgatggtta ctaggaaaat gtttttggca ccttcaaatg ctcgcaaata gtcatctggg
     6121 cttggacaag ccgattttga agcttctgca gttgcataca tggtttccat catttggtca
     6181 atatcgagac tggcgtcatc aacaaagacc tgatcagcta cttgaatggt taaggggaca
     6241 cttacaaagg ttgtgttaat agctggtgtt ggcagttgac gataatcaca accagagtca
     6301 gcaataattt tccaagtcat agaaattctc catctttgtc agttatacat tgacaaaggt
     6361 tctgtctttt tttacaatta tatcatgaaa acccttgaaa caaaagcatc atccgtctgt
     6421 tgtgacaagt agaaagaaaa tgttatgtct gaacgtagaa tctctgaaaa gtctcttgaa
     6481 aatctcagaa aatcaaacca agaatccaat ttattaacca gagaagccat tgaaacagcc
     6541 ctcttgcaac tcttggaaaa aaaggagctg accaagatta gtatttctga attggtcaaa
     6601 cgtgcaggtg tttcgcgtgc ggccttttat cgcaattatg actccaaaga ggaaatttta
     6661 gagagcgtct ttaaacgaac tgtccacaat attatggaac agctgcatca ttacgattta
     6721 aagacagacc tttatttggt ttgggttcac cttttccggg aggccagaaa ggaagctaga
     6781 gtgattcaat tggccttgga ttaccatctg gaaaaaatct ttgtccaagc catgcaggaa
     6841 tttctagaaa aataccatgg gaaatcaaaa ggtgtcagct cttatcttca ttccttctgg
     6901 agctcagcca tcgtctctgt ccttctaaaa tggatcaagg atggcatgaa ggtaccagct
     6961 gaaaagattg cagatttagg tttaccattt tttaaaaaat agagaaaaag gagaaaagag
     7021 atgactgaaa aaagactggc ctgggatgag tattttgcag cccaagccct actaattgcg
     7081 aatcgttcca cttgtaaacg tgctaaagtg ggcgcgattc tggtaaaaga taataaggtt
     7141 atttccactg gttacaatgg ttcggtgtca gggactgagc attgtattga tcacgaatgt
     7201 ttggtcattg aaggccactg tgttcgcacc cttcacgctg aggtcaatgc tatccttcaa
     7261 ggtgctgagc gtggtgttcc caaaggcttt acagcctatg taacccattt tccttgtctc
     7321 aactgtacaa aacaattgct tcaggtcggc tgcaagcgcg tggtttatat caaccagtac
     7381 cgaatggacg actacgccca atacctttat caagaaaaag ggacagaatt gacccattta
     7441 ccacttgaga cagtacagac agctcttaaa gaggctgatc taatgtaaaa attatcaaaa
     7501 ataaatggct tagaaagatt tttaaaccgt tttttggtat aataagaaga ataaattgaa
     7561 agaaggaatt ccaaaaatgg gaaaaattga agttattaat cacccactga ttcaacacaa
     7621 attgtcaatc ttgcgtcgta cagatacttc tacaaaagct tttcgtgagc tagtagatga
     7681 gattgcaatg ttgatggggt atgaagtact tcgtgatctt ccactagaag atgtggaaat
     7741 cgaaacacca attacaaaaa cagttcaaaa acaattggca ggtaagaaat tggccatcgt
     7801 cccaatcttg cgtgcaggta tcgggatggt tgatggtctc ttgagcttgg ttccagctgc
     7861 taaagttggc cacatcggta tgtaccgtga tgaagaaaca cttcaaccag ttgaatactt
     7921 ggtgaaattg cctgaggaca ttgaccaacg tcaaattttt atagtagacc caatgttggc
     7981 aacaggtggc tcagcaatct tggctgttga ttctcttaaa aaacgtggcg catcaaatat
     8041 caaatttgtc tgccttgtat ctgctccaga gggtgtaaaa gcccttcaag aagctcatcc
     8101 agatgtagaa atctttacag cagccttgga tgaacgtttg aacgaacacg gttatatcgt
     8161 tccaggtctt ggagatgctg gagaccgctt gttcggtaca aaataagatc gaaaagaaga
     8221 atgacttgga aaagtatttc cagtcacgaa aggaggttgg gtttttgttt ctgtctaatg
     8281 aaagcagagc aaaaatttga ccttttttga ccaagatatt ataatagtct tatcttcagt
     8341 catttgacca acaaaattaa aactcaaaag gagaaatgaa tgattcctgt agttattgaa
     8401 caaacaagcc gtggagaacg ttcttacgat atttactcac gtcttctcaa agaccgcatc
     8461 attatgctga caggtccggt tgaagacaat atggctaact ctgttattgc ccaattgctt
     8521 ttcttggatg cccaagatag tacaaaagat atttaccttt atgtcaatac accaggtggt
     8581 tctgtttcag ctggtttggc aatcgtagat accatgaact ttatcaaggc agatgtccaa
     8641 accattgtta tgggaatggc tgcatctatg ggaacagtca tcgcatcaag tggagcaaaa
     8701 ggcaaacgtt tcatgcttcc aaatgctgaa tacatgattc accaaccaat gggcggtaca
     8761 ggtggtggta cccaacaaac tgatatggct atcgctgcag aacacttgct caaaactcgt
     8821 aataccttgg aaaaaatctt ggctgaaaat tcaggtcagt caatggaaaa agtccatgca
     8881 gatgcagaac gtgataactg gatgagcgcc caggaaacac ttgaatatgg ctttattgat
     8941 gaaattatgg ccaacaattc attgaactaa tgatgataga aggcaaactc gactgggttt
     9001 gctttttttg gtataatagg gagagatttc ttagaaagag gatttatcat gtttgaaaaa
     9061 gtcaatcgct ctggcttgat tatctatctt tactataatc gtgatgccaa aaaactgcag
     9121 gattatggag atattaccta tcattccaag aaacatcgtt acttacaact ctatgttcca
     9181 actcaagaag tggagcaatt ggtcggacgc ttgagcaagg aaaaatttat taaaaaagtt
     9241 agggtttgtc atatccaaga gttggaaaca ccctttgtgg gcaatcttta tcgataggaa
     9301 aacgttatca tcgaaaaagt tcaaggaaaa cattgacaat tttctgataa ttcggtatat
     9361 tcttaacaga ctatttaaga aataaggaga caaaaagatg aagaaaaaat ttgccctatc
     9421 gtttgtggcg cttgcaagtg tagcacttct tgcagcctgt ggagaagtga agtctggagc
     9481 agtcaacact gctggtaact cagtagagga aaagacaatt aaaatcgggt ttaactttga
     9541 agaatcaggt tctttagctg catacggaac agctgaacaa aaaggtgccc aattggctgt
     9601 tgatgaaatc aatgccgcag gtggtatcga tggaaaacaa atcgaagtag tcgataaaga
     9661 taataagtct gaaacagctg aggctgcttc agttacaact aaccttgtaa cccaatctaa
     9721 agtatcagca gtcgtaggac ctgcaacatc tggtgcgact gcagctgcgg tagcgaacgc
     9781 tacaaaagca ggtgttccct tgatctcacc aagtgcgact caagatggat tgactaaagg
     9841 tcaagattac ctctttattg gaactttcca agatagcttc caaggaaaaa ttatctcaaa
     9901 ctatgtttct gaaaaattaa atgctaagaa agttgttctt tacactgaca atgccagtga
     9961 ctatgctaaa gggattgcca aatctttccg cgagtcatac aagggtgaaa tcgttgcaga
    10021 tgaaactttc gtagcaggtg acacagactt ccaagcagcc cttacaaaaa tgaaagggaa
    10081 agactttgat gctatcgttg ttcctggtta ctataatgag gctggtaaga ttgtaaacca
    10141 agcgcgtggt atgggaattg acaaaccaat cgttggtggt gatggattca acggtgagga
    10201 gtttgtacaa caagcaactg ctgaaaaagc atcaaacatc tactttatct caggcttctc
    10261 aactactgta gaagtttcag ctaaagctaa agccttcctt gacgcttacc gtgctaagta
    10321 caatgaagag ccttcaacat ttgcagcctt ggcttatgat tcagttcacc ttgtagcaaa
    10381 cgcagcaaaa ggtgctaaaa attcaggtga aatcaaggat aaccttgcta aaacaaaaga
    10441 ttttgaaggt gtaactggtc aaacaagctt cgatgcagac cacaacacag tcaaaactgc
    10501 ttacatgatg accatgaaca atggtaaagt tgaagcagca gaagttgtaa aaccataata
    10561 gaaaaatgtt gaaataggga atgagccttt gactcactcc ctgtttcgat atttaatact
    10621 cttcgaaaat ctcttcaaac t
//
LOCUS       DAVMGZ010000061        10606 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000061 DAVMGZ010000000
VERSION     DAVMGZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.062"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(55..1377)
                     /locus_tag="VYQ93_001271"
     CDS             complement(55..1377)
                     /locus_tag="VYQ93_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV3335957.1"
                     /translation="MLELLKSIDDFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKHAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPVITALVLSVFVAIAVFGGLKSISKVSTTVVPFMTIIYI
                     LGTLIVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(1468..1556)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1720..4056)
                     /locus_tag="VYQ93_001272"
     CDS             complement(1720..4056)
                     /locus_tag="VYQ93_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV3335958.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(4160..4708)
                     /locus_tag="VYQ93_001273"
     CDS             complement(4160..4708)
                     /locus_tag="VYQ93_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV3335959.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(4705..5007)
                     /gene="zapA"
                     /locus_tag="VYQ93_001274"
     CDS             complement(4705..5007)
                     /gene="zapA"
                     /locus_tag="VYQ93_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV3335960.1"
                     /translation="MANLNRFKFTFGKKTLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5094..5975
                     /locus_tag="VYQ93_001275"
     CDS             5094..5975
                     /locus_tag="VYQ93_001275"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV3335961.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQITPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            5979..6593
                     /gene="lepB"
                     /locus_tag="VYQ93_001276"
     CDS             5979..6593
                     /gene="lepB"
                     /locus_tag="VYQ93_001276"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV3335962.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            6721..9087
                     /locus_tag="VYQ93_001277"
     CDS             6721..9087
                     /locus_tag="VYQ93_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV3335963.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGITGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDRVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(9135..10418)
                     /locus_tag="VYQ93_001278"
     CDS             complement(9135..10418)
                     /locus_tag="VYQ93_001278"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV3335964.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYSKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         3016 a         2424 c         2149 g         3017 t
ORIGIN      
        1 aggctgataa aaattattta taggcaaaaa aatcacacga gctgtgtgat tccattattt
       61 gtcaaaatac tttttagttt cagcaataac gactggcgac aagaccaaga gggcaatcaa
      121 gtttggcaga gccatcaagg cgttaacgat atctgcgata atccagacca tatccaactc
      181 gataaatcct cctaacaaga ccatgagcac aaaaaccaca cggtagagcc agataaagcg
      241 aaccccaaag aggaactcaa aacagcgttc tccgtaatag ttccaaccta gaatcgttgt
      301 aaaggcaaaa agtacaagga agatggtcaa gagggcaggc ccaaagtgtg aaaagactgt
      361 tgagaaagct gactgagtca aggcaacccc attcaagtca ccactccaaa ctccagttac
      421 caagatggtc aaaccagtta gagtacaaat gatgagggta tcaataaagg ttcctgtcat
      481 ggaaatcaaa ccttgctcta ctggttcatt tgtcttggca gctgcagctg caataggagc
      541 agaacccaga ccagattcgt ttgagaacac accacgcgcc acaccatttt gaatagccat
      601 ccgaacgcta gcaccagcaa atccacctac cgcagcaagg ggactaaaag ctgaggtaaa
      661 gactaaagcg attgtgccag ggatttttcc gatattaaag aaaataacta taagagttcc
      721 taagatataa atgatggtca taaaaggaac aacagtagtt gaaaccttag aaatagactt
      781 gagtccacca aagactgcaa tcgctacaaa gacagacaag acgagagctg tgatgactgg
      841 cgaaatcgtc gttgtatttt ggatagattc tgtaatcgag ttgacttggg tgaaggttcc
      901 gattcccaag agagcaacca atactcctgc tactgcaaac aaaacagcaa gtggtcgcca
      961 cttttctccc atccctagaa ggatatagtg catgggacct cccgctactg caccatggtc
     1021 gtccttggtg cggtatttga tggccaagag tccttccgca tgcttggtag ccattccaaa
     1081 gaaagccgcc atccacatcc aaaatagagc tcctggtcca ccaaccttga tagccgtcgc
     1141 aactcctatg atatttcctg ttccaacagt tgatgccaag gctgtacaca gagctgcaaa
     1201 actggataca tcaccatgtc ccttatcctg gataaaaata agctgaaagg ccttgggcag
     1261 acgcaaaacc tgcaagagtc ctagccgcat ggttaggtaa atccctgttc cgaccaataa
     1321 aatcaagagg ggcggtcccc aagcaaaatc atcgattgat ttaagcaatt ctaacatttc
     1381 cttctcctat cgtttcaacc ccaaaagaaa gagcacatgc aagatacatg tactctggaa
     1441 tgcttagata aatgctaaaa agcggtctat cctagctctg tccttttacc tgagagtttg
     1501 agcagttgcc tgccttgccc cttcggtgcc tttacggtct ctccagagtt ccgtccattt
     1561 acagtcatgg aaaatcaaac gattccccac ttctattaaa cttcattcgg tgttggtatt
     1621 taattgattc taattttaca aaaaatgttg gcttttgtca atgtgtttat tagtaaaaat
     1681 tagttcaaca gtttttactt tataaagtcc agaatactgc tatcctttaa aagtgacaat
     1741 agtcgcacca ctgcctccag cattttgtgg ggcatagccg aaactcttga catgtttgtt
     1801 tctttgcaag tatttggtaa ctccttcacg gatgactcct gttccgatac catggatgat
     1861 atcaacttga gccatattgt taagcaaggc ttggtcgatg aaggtatcta gctcattcat
     1921 ggcttcttca tagcgcttgc ctcgaagatc cagtctagct tgaggtcctc gcccagaagt
     1981 tcgtttcaca acattgacct gtttcttctt gactggtttt tcttgctggg cttgaacaag
     2041 atcaaactct ttctcttcca aggtcatctt aatcaagcca acttgggctt cccagcggcc
     2101 gtccttgagt tgactggtca aggtaccacg ctgaccataa ctgagaacca cgatatcatc
     2161 tcccaccttt ggagctcgtt ttttcttggc cttttgaagg accttgtttt tagacaagtc
     2221 cactttttca ggagccaatt ttttcaactt ggccttggct tcaatgattt cgtggggctt
     2281 gagttgggat ttactgtgga gatttttgag aatctggtca ctttcactta gggccatatc
     2341 cacaatctca gcagcctgtt cacgcgcctt gttaagctcg gtttcctttt cacgattaag
     2401 ctcgttgtag agttttttta gcgcacggtt catcttgaga ttttcttgct ccacctcacg
     2461 gatattgtcc aaacgtttgc ggctttccag cgtctgctct tctaattgct caatgatacg
     2521 attgacgtca ttgtcctgat cgatctgctg actggcatct cctacgataa cttcagatag
     2581 gcctagacgt ttggcaattt caaaggcatt acttcggcca ggaacaccct gcataaagcg
     2641 ataggttggg cgaagagttg cagtatcaaa ctccatactg gcattttgca caaaggctgt
     2701 ctcaataccg taggccttga gttctggata gtgggtcgtc gccatggtct tgatttgacg
     2761 caggcgaagg tcctccagaa tagccatggc aagggctgct ccctcttggg gatcagtacc
     2821 agcccccaac tcatccaaaa gtaagagtga atgttggttg accttgccaa gaatatccac
     2881 gatattggtc atatggctag agaaggtaga caagctctgc tcaatagact gctcatctcc
     2941 aatatcagca aagatttctt caaaaatacc aacacgactt cccttgtctg ctaaaatcgg
     3001 caatcctgac tgggccatga cctgtgtcaa gcccagagtt ttgagcatga tggtcttccc
     3061 acctgtattg ggacctgtaa tgacaatagc tgttaaatct tgaccaaaat agacatcatt
     3121 tgcgacggca tttttgacca aaggatggca gacatggagc agttgaatct cttgattttc
     3181 tgacagctga ggcacgactg cttgtctttc ttggataaat cgaaccttgg cacgaatcaa
     3241 gtccagatga ccgataatcc aagcgtcatt agcaatctca gccgcatgag ggcggacacg
     3301 ctcagaaatt tcttggagaa tgcgaagcat ttcatagcgc tcatctgctc gcagactagc
     3361 aatttcttcg ctcagtttga ctacctcacg tggttcgata tagacggtgt ttccactagc
     3421 agaaatatca tgaacgacac ctgcaatctt attgcggtag gtgtttttga ctggtaaaac
     3481 ctgacggcca tttctgctag caacaattcc ttccgtcaac atctgcgctt tttgcttgag
     3541 taagtcttgt aaaacatcgc gcacctgact ctcgctatca tgtatttttc gacggattcg
     3601 cgccaattct tcactggcaa aattttcaat gaaacccgca tcattaaagg cctgaagatt
     3661 tccttgtaat tgcggaaaat catgtaattt ctcaaaccaa agggctaatt cttccaagct
     3721 gacattttcc agattggcgt aaaaattttg aagttctcgg ctggcaagaa gcacgcgttt
     3781 caagagtagg aactcctcga tattgagatc cgctcccatc tccaacctct tgcagactcc
     3841 tgcaatttcc ttagttgaga gaatagtaaa atgcggttgc tcgacgaaaa gagcctgcat
     3901 ttctttcatc tcagcaaaag cctgtttgat tttatctgct ttggcagtcg gagccagctg
     3961 tctcaattgc tccaagccct gctcggtcaa caaatgaggc tcaaacaagg ccttgacctt
     4021 atcgaactct aatgtttcta atattttctt attcatcttt atttccttgt ctttgaatgt
     4081 ctaggtgtgg tagcttttta cattctgata gattcgagtc aaatctgtaa acaaagggct
     4141 aggaaaactc ctgccctttt tatccgatta aatttgtcac ccagatttgt ttgagccaac
     4201 tggttgttat cggtatgctc tggatgatgt gttttgcgac gatactcttt tcaagaggat
     4261 tttgtataac tgccatgggg atggtcgcca agattgtcaa ggccatttgc aagacaaata
     4321 aggtcaccaa catggacaag atacctgctg aaacttggaa caacttacca cccagttttt
     4381 tgctaggaat caagtgtaag agaagaccaa gcaaacgacc aatactatag acaatcccaa
     4441 atacaagcaa gtagccgata cctgcataaa agaccttatc cagctgaaag agttgatccg
     4501 atgggaaaaa gaaagtcccc tgaccttcct gcgaatttgc ataagggagc aataaatgga
     4561 attgctctcc aagcccctta taaaactggc cagccataaa agccgatgcc atggctgaaa
     4621 tcaggtaata aacctgtaag agcaggcctc tccgatagcc gatataaaat ccccaaacca
     4681 agaccaatag aagaaggaat gaaatcataa ggaatcctca atcttgctct gttcttgctt
     4741 acaagtcaca agcttgtgac ggagttcttc tagctcttgc tccttatcgt caaattcaat
     4801 ctcacggctg agctgagttg ataaacagtt gactgccaac aaaagagcga ttgtttcatc
     4861 atctgcgcta ggcatttgtt ctttaattgc ttggtatttt tctgtcgcaa ccttagcgat
     4921 ttcctccata aaaaggttat catgttcgct tgtcaaggtt aacgtttttt tcccgaatgt
     4981 aaatttgaat cgatttagat ttgccataaa attcacctca cgatattata ccaaaattcg
     5041 ctaattttgt cagtttttac aaattttact gcttttgtgg tacaatagaa actatggcaa
     5101 gtatcacact cacaccaagc gaaaaggata ttcaggcttt tcttgaacac tatcaaacca
     5161 gtctggctcc tagcaagaat ccctatatcc gctacttttt gaaactacct caagcaacgg
     5221 tttctatcta tacttctgga aaaatcttgc ttcagggtga aggggctgaa aaatacgcca
     5281 gtttctttgg ctatcaagct gtagagcaaa ccagcggaca aaatcttcct ttaattggga
     5341 cagatgaggt gggaaatggt tcctactttg gtgggcttgc agttgtggct gcctttgtca
     5401 cacctgacca gcacgacttt ttacgaaaac tcggtgtggg ggattctaag actctgaccg
     5461 accaaaagat ccgtcagatt actcctattc tcaaggaaaa aattcagcac caggcactcc
     5521 ttctctcacc cagcaagtac aacgaggtca tcggagaccg ctacaacgct gtttcggtta
     5581 aggttgccct ccataatcag gctatctatc tcctccttca aaaaggtgtt cagcctgaga
     5641 aaattgtgat tgatgccttt accagtgcta aaaattatga caagtacttg gcacaagagg
     5701 ccaatcgttt cagcaatcct atcagcttag aagaaaaggc tgagggcaaa tacttgtctg
     5761 tcgcagtttc ttctgtcatt gcgcgtgatc tctttctgga aaatcttgaa aatttgggac
     5821 gagaactggg ttatcagctc ccaagtggag ctggaacggc ttctgacaag gtggctagcc
     5881 agattttgca agcctatggt atgcagggac tcagcttctg cgccaaattg cactttaaaa
     5941 acactgaaaa agcgaaaaaa cgcttagaaa ggtaagttat gaattcattt aaaaatttct
     6001 taaaagagtg gggattgttc ctcctaattc tgtcattact agctttaagt cgtatctttt
     6061 tttggagcaa tgttcgcgta gaaggacatt ccatggatcc gaccctagcg gatggcgaaa
     6121 ttctcttcgt tgtaaaacac cttcctattg accgttttga tatcgtggtg gcccatgagg
     6181 aagatggcaa taaggacatc gtcaagcgcg tgattggaat gcctggcgac accattcgtt
     6241 acgaaaatga taaactctac atcaatgaca aagaaacgga cgagccttat ctagcagact
     6301 atatcaaacg cttcaaggat gacaaactcc aaagcactta ctcaggcaag ggctttgaag
     6361 gaaataaagg aactttcttt agaagtatcg ctcaaaaagc ccaagccttc acagttgatg
     6421 tcaactacaa caccaacttt agctttactg ttccagaagg agaatacctt ctcctcggag
     6481 atgaccgctt ggtttcgagc gacagccgcc acgtaggtac cttcaaagca aaagatatca
     6541 caggggaagc taaattccgc ttctggccaa tcacccgtat cggaacattt taagaaacct
     6601 aagaggccga gaatcaccaa tctcagcctc ttcttctatc gtgagaaaat gattggtaac
     6661 tatctaaact taccagaaca gaaacacctc aactctcacc tattcatgca aaggaatttt
     6721 atggaagttt atttttcagg aaccattgaa cggattattt ttgaaaatcc cagcaatttt
     6781 tatcgcatcc tcctcctaga aatcgacgat acggacgcag aggattttga tgattttgaa
     6841 atcattgtca caggaaccat ggctgatgta attgagggcg aagactatac tttttggggg
     6901 caaattgtcc agcactccaa gtatggagaa caactgcaaa tcagtcgtta tgatcgcgca
     6961 aaaccaacta gtaagggctt ggtcaagtac ttttcaagta gccatttcaa ggggattggt
     7021 ctcaagacag ctcagaaaat cgtggatacc tatggcgaaa ataccattga cgaaattttg
     7081 caacacccag aaaagttaga aggcatcaca ggactctctg ccaaaaatcg cgaggctttc
     7141 gtctccactc tccgtctcaa ctacggaacg gagatggttt tgtccaaact agccaactac
     7201 ggcattccca acaaactagc ctttcagatt caagactttt acaaggaaga aacccttgat
     7261 gtggttgaaa attatcccta ccagctggtt gaggatatca agggtttggg ctttaccatt
     7321 gctgaccaac tagctgagga actaggcatc gaaagtcagg ctcctgaacg cttccgcgcc
     7381 ggtctagttc acagtctttt tcaggcctgt atggaaacag gggacaccta tgttgaagca
     7441 cgggatttgc tggaacaaac ccttactctc cttgagtctt cccgtcccgt ggaactagac
     7501 cccagccaag ttgcccaaga gctctcctac ctgatcgaag aagacaaggt tcagcagatt
     7561 gataccaaga tctttgacaa cagcctcttt ttcgctgagg aaggcatccg cagtcacttg
     7621 gttcgtatcc ttgaaaaagg aaaacagaag agtcatgatt tagaaaccat tcaaaaacat
     7681 atcactacta tcgaggaaga gctgggaatt gagtatgata acattcaaaa acaggctatc
     7741 tgtgatgcta tccagaataa ggtctttatc ctgacaggtg ggcctggtac tggtaagaca
     7801 actgttatca atggaatcat tgctgtttat gcccttttag aaggacttga cttcaggaag
     7861 aaaagcaatc tgcccattct tcttgctgct ccaactggtc gagctgctcg tcgcatgaat
     7921 gaattgacag gtttgcctag cgcgaccata catcgccact tgggaatgac aggtgacgat
     7981 gataccagtc atctggaaga ttacctagat gctgacttta tcatcgtgga tgaattctct
     8041 atggtagata cttggctggc caatcaactc ttctccaata tctcttctaa cagtaagatc
     8101 ctcatcgtag gtgacagtga tcagctacct tctgtcagtc ctggacaggt tctagcggat
     8161 ctacttcata ttcctttgat tcctcagact cgcttggaaa aaatttatcg acaaagcaaa
     8221 gaatcaacca tcgtcaccct agctagtcag attcgacagg gcatcttgcc agctgatttc
     8281 acccaaaaaa aagctgaccg ttcctacttt gaaattgcta gtggccatat tcctgccacc
     8341 attgaaaaaa tcttaggtgc cgccctcaga aatggtattc ccgcccgtga tatccaagtt
     8401 ctggctccta tgtaccgagg gacggcaggg attgatgcta tcaatcagct catgcaagac
     8461 ctgctcaatc ccccacaaaa agatcaactc agttttgaag ctcctcagtg tcactatcgt
     8521 aagagagaca gggtcattca tttggttaac gatgctgaaa tcaatgtctt taatggggat
     8581 ttaggagcta tcacagacct gattcctggt aaatacaccg aatcgaaaca agacgagatt
     8641 gtcattgatt ttgatggtaa tgaagtctct tacccccgta acgaatggta caagattcgc
     8701 ttggcctatg ccatgagtat tcataagtct cagggaagtg agttcccagt tgtcatccta
     8761 ccgattacta gtgctagccg acgtatgctg gagcgaaatc tcatctacac agccattaca
     8821 cgtgccaaaa gcaaactcat cttactaggc gaattacaag ccttcgacta tgctacccaa
     8881 cacatcggaa ctgcccgaaa aacctatctg attgaacgct tcagtgatct actggagaat
     8941 gttgaagaaa agcaacaagc tgtctctgaa acagtcacat caagtgcctc tgaacaatcc
     9001 tacatcctaa ccgaagaaaa ctgggaccgc atcccagcca tgattgggat tacagacaca
     9061 gacctcaaag agatttttgg aaaatagtgc atcagaaaac ccaccaattc aggtgggttt
     9121 tctgtttatt aagattattt tactgttgct gtgcttgtga tcaattcaac agcttttttg
     9181 atagtgatat catgtttcaa catgtcagct gaaagcaagt tttgaacttg tgcaacttcc
     9241 atgttgtagt ctgctgccaa ttgctcaact tctttttgga tttcttcttc tgaagcatca
     9301 aatccttcag ctttggcaac tgcttcgata acaaggttag tcttagtacg tgactcagct
     9361 tctgcttggt attggttgtg aaggtcttct tgagtagttc cagtgatttg gaagtacatg
     9421 tcagggttga tcccttgacg ttgcaaattc ccaaggaatt catttactga acggtgaact
     9481 tcttcatgga tcatttcttc tggaagttct acgatttcag cattttctac agctgtatca
     9541 attgctgcac cttcaactgc atctttgtaa gcttcttctt tagcagcagc caattctttg
     9601 ctgtattttt ctttcaagtc agcaagtgtt tcaacttctt catcaatgtc ttttgcaagt
     9661 tcatcgtcaa gagccggaac ttctttagct tttacttcgt ggatagttgt cacgaattta
     9721 gcttctttac ctgcaaggtc ttctgcttgg tagtcttctg ggaatgttac gataacatca
     9781 acggtttcgc cagctgagtg acctaccaat tggtcttcga aaccagggat gaattgacct
     9841 gaaccaagtc caagtgagaa gttttcacct tttccaccgt caaattcaac accgtcgata
     9901 gaaccaacga agtcgatcac aacagtgtcg ccgttttcag cagcagcttc cttgataacc
     9961 aattcagcca ggttgttgcg ttcgcgttcg atacgctctt cgacatcagc gtcagttact
    10021 tctttttcta catcaactga tacttcaagg tttttgtagt cacccaattt tacttcaggt
    10081 tttgtaacga cttcagcagt gataacccag tcttgacctt tttccattga agttacgtca
    10141 atttttggtt gggcaaccac ttcaagacca gcttctttta cagctgcttc ataagcgttt
    10201 ggcaaaagtg cgttcattgc atcttgataa agagcttctt caccaaattt ttggtcgaag
    10261 atagggcgtg gaaggtgacc tttacggaaa cctggaacat taagagattt cttcactgac
    10321 ttgaagacac ggtccaattc tggtttgatt tggtcttgag agatagtgaa agtcaagaca
    10381 ccacggtttg tttctttgtt ttcaaatgat acagacattc tgtcatttct ccttaaaatt
    10441 ttttaaatac agtctattat aacattttct ataaataaaa tctagactaa tgcagacaat
    10501 tatagacaac aagccttttt cctaactaaa acactcataa agtatagaca attttagact
    10561 actttggata tcttttggtt agtcattttc caaaactaat ttatct
//
LOCUS       DAVMGZ010000062        10525 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000062 DAVMGZ010000000
VERSION     DAVMGZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.063"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(34..1458)
                     /locus_tag="VYQ93_001279"
     CDS             complement(34..1458)
                     /locus_tag="VYQ93_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV3335965.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHTAKQIVAGAF
                     SYSGQRCTAIKRVFVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2003..4282)
                     /gene="pulA"
                     /locus_tag="VYQ93_001280"
     CDS             complement(2003..4282)
                     /gene="pulA"
                     /locus_tag="VYQ93_001280"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV3335966.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4401..6359)
                     /gene="ligA"
                     /locus_tag="VYQ93_001281"
     CDS             complement(4401..6359)
                     /gene="ligA"
                     /locus_tag="VYQ93_001281"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV3335967.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6452..7630)
                     /locus_tag="VYQ93_001282"
     CDS             complement(6452..7630)
                     /locus_tag="VYQ93_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3335968.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7710..8573)
                     /locus_tag="VYQ93_001283"
     CDS             complement(7710..8573)
                     /locus_tag="VYQ93_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV3335969.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQKAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8576..10477)
                     /locus_tag="VYQ93_001284"
     CDS             complement(8576..10477)
                     /locus_tag="VYQ93_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV3335970.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKYSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3158 a         2332 c         1891 g         3144 t
ORIGIN      
        1 aaaaccagga aaacaatttc ctggttttac acgttatttc acatcaaaaa caatggattt
       61 gacatttgtc atcgcttcaa tgctatattt aattccttgc actccagctc cagaaccttt
      121 gacaccaagg aatgggaaat tatctggacc acgctgggtt ttattattaa tgtggactgt
      181 acctacttca agtttttcag caatttcaaa ggcttttttg aaatcatttg taaagactga
      241 tgattgaagg ccgaattcag attcgttggc aaaggcaata gcttcctcta cactagccac
      301 acgaatgatt ggtaaaacag gaccaaatgg ctcttcccat gccactttca tatcttttgt
      361 aacttggtca aaaagcactg gccagagaag attgccctca cgtttgattg gtgtaagagc
      421 ctgagcttct ttttcttgtg catcctcaat caagccccaa atgaagtcgg ctgaagcatt
      481 gtcaataaca ggtgtaatat cagcattgtc aaatggatca ccaactgtta atttagaaac
      541 ttcttcctga agcaaagtag ctaatttatc tgctacactt tcgagaacaa agacacgttt
      601 aatggccgtg caacgttgtc ctgagtagct aaaggctccc gcaacaattt gcttggcagt
      661 atgttccaaa tctgcatctt ctagtacaag agctgcatct ttcccaccaa gttccaacat
      721 gataggacgc ataccagcta aacgaccaat acgttctcca ataggagttg aacctgtaaa
      781 gttgatgaag ttgacttctt tgtgctcaat gatataatcc ccaatttctg aaccacgacc
      841 tgtaatggtg ttgaaaacac ctgccggaat ccctgcttct tcaaatgctt tagccaacaa
      901 gagtccagaa atggaacctt gtgttggtgg cttaaacatg accacattcc ctgcaatcaa
      961 ggcaggtgca attttagaag cagataaatt aactggataa ttaaagggag caatcgctag
     1021 cacgatacca actggttcac gacggacaac agccagtttg tttttacttg ctgcctcaaa
     1081 accaccacct tccattgctt gtccagtgat acggagacct tcctcagcag cataacgaat
     1141 caagtctgct gtacgcacta cttctccaat tgctgcttta atcccttttg ctacttcttt
     1201 ggcaaggata gtaccaattt cttccttatc gcgttctaaa atagctgctg ttttatgtaa
     1261 ataagccgca cgttcaactg ctgataaagc tcgccatgct ggcagggctg cacgcgcagc
     1321 ttgcatagcc tcatcagctt cagtctgagt catggctgga actgtaccca attcttcttg
     1381 attgattggt gaataaatcg taatttcttg ttcagatgat ttccattttc catttactaa
     1441 attctgatat cttgtcaaat tcttgtcctc ctgaaaatga ttaagatata ttttaccaaa
     1501 ttttcagaaa atcacaaccc tttttgcaaa atttttgaga ttattttcac aaacttgatt
     1561 tttcaaagta tactcaataa aaataaaaaa aatccactac gtcaaggcga ggctaatgtg
     1621 gtttgaagaa attttcgaag agcgtgaatg agtatcatct atagtaaaat aaaaaaactg
     1681 aacaatttgg ttggggacag ccaaaccaat ttctcacaat gtttcagaaa caagggtgtg
     1741 ctactccaat ttcagcctac tataactgtc atagattgct gaaacaaagt ctaggtaaaa
     1801 gtcttcataa taaaaagacc tcctatcaag tgttcaaaaa ctttgatagg aggtcttgtt
     1861 ttgtgaaaat atttatcaaa ttttctatac aagtgagctg ttagccaggt tctttctatt
     1921 ctttcaattt caatgaatgg attttttact aatactcata actgggaatt tgtctgtgta
     1981 aaaatagcga gatagatggt atttataaaa cactcaagac agctagacta atatcattta
     2041 aaacattatc ttcttttgag cgactgttgg ttaccaacat agctaaattt cctgcatttt
     2101 caaattgata gggttctgat ttagcattca caaccaccaa gaggtgttct ttgccgtgaa
     2161 cttcatagat aaggtagccg ctatgttcaa tcgcagaatg cacaaagaca tgatggtaaa
     2221 tttcatcata gctagagtaa gaaaaggcac cagtttttgt cttcaatcgg atgacttgac
     2281 ggataaactc aatactgtct tgacgctcat taatcaagtt ccagttcact tggttcacac
     2341 tgtcaggagc attatagcta ttcatcgcac gctctctatc atcatgggtc aactcaccat
     2401 tttcaccagt cgcaaccagt ttggtacgac caaattcttg accgatttcc ataaaggcca
     2461 tcccctgcat gagcagattc atggctgtgg cagtttcgac cttgcgcatg atttgctctg
     2521 aactttggtc tggatgaagg gttgccaata aatcgtgaag attgtaattg tcatgggctt
     2581 ctacatagtt aagcacctga tttggatgtg tatagcttcc taattcacga cttcctagga
     2641 ttgctttagc tagaattggc tctgtcgcag caccactgac aaaacctgac ttgatagcac
     2701 cataaacttc tccccctttg acagcatcgc gctgattgtc attaaagaaa ccaatatttg
     2761 gcatctggta ggcattgtcc ttcttggcct tatcataagg ggcaagacct gttcccatat
     2821 cccatccttc tccatagagg ataatgttgg agtcgatttc atccaagctt tgacgaatca
     2881 tctgcatggt cttgacatca tgaatcccca tcaagtcaaa acggaagccg tcaatattat
     2941 attcctgcac ccagtataga agagaatcaa tcatatactt gcgaaacatt tcgtgttcac
     3001 tggctgtttc atttccaaca cccgttccat tctggaaggt accatctgga ttcatacgat
     3061 aatagtaatc agggactgtt gtttggaatg gtgcatcaac aactgagaag gtatggttat
     3121 agactacatc cataatgact ccaatacccg catcgtgata agcttgaacc atcaccttca
     3181 aatcacgaat gacctgagct ggatcatctg gattagttga aaaactagtt tctggcgcgt
     3241 tatagttttg tggatcataa ccccagttgt aggttacatt tccatcctca tcgtattctt
     3301 tatgacggtc tgcaattggt tgcaattgaa cataattgta gcccagcttc ttgatgtaat
     3361 caaaagcagt tgactggccg tattggttaa ctgttccagc ctgagcagca cccaagaaag
     3421 ttcctcgaag atgttcatct acacccgatg taggtgattt agtcaaatca cgaatatgca
     3481 tttcacagat aactgcctta catggatttt ccaagcgcca agtagcctcc gaaccgtgct
     3541 taacctcgaa gttttcaact tgcttttcta catggctcag aatagctgaa cgtttgccat
     3601 cagggctggt cgcgattgta taaggatcac gtgtcagtgt ttggtgatga gggaattgga
     3661 cttgatactg ataagcctta cctaccaaat cttcctcaac atccaaactc cagacaccga
     3721 ttgtattgtc cttatgatta taagagtagc tattgcctct tttcatctca aaagtcttcc
     3781 aaacgggtgc atcattagca gctgattcat aaacgacaac ttgcacttct gtcgctgtag
     3841 gtgaccagag ggaaaaatga gcctgattgt cctctacacg gcaacccaat tctccttggt
     3901 aaccccaatg atggtcaaaa ctagcactgt taatggcctt atcaaaggca aaaggatttt
     3961 gatttttata gaaaggactg gcaatagcag gattttcaga gtaataaatc ctatcatcgc
     4021 cttccaaaat ccagacctct gttaataggg gatagtgatt aaaacggata gaatattctt
     4081 tactagtttg acctgtatga accacaaaat tcaagctttc tataacatgt gaacttgggt
     4141 gttcaaagct aaataaagct ccaaaataat cttctttgta ggttagcaaa tcaattcgtt
     4201 gatcctgact ttttacaaag gagcaagtgt catattctcc attcttacga tggtaatgaa
     4261 tgcgcatagg gtagttatac attttttatt ttttattttt cctttttact ttgtttctat
     4321 ttcactaata aatttttgtc aatctcgtct caattaacag acatagtcat attctctaaa
     4381 ctctgttttt aaacgatcca ttacaaactt tctagccatg cctcatctct gacctggata
     4441 ccaagttctt gtgctttttg cagtttactt ccagcgtctg cacctaccac gacgaggtcg
     4501 gtctttttag aaatactacc tgtcactttg gcacccagac tttcgagttt acttttagct
     4561 tctgagcgct tgagtcgttc caattttcct gtcaatacca cggtcaaacc tgacaaggcc
     4621 gcatccgcta ctaccgtctg tcctttatag tccagattga ccccagtttc tttcaattct
     4681 ctgagcagaa tttcagagcc ttctgtcgca aaataagtct gaagactttt ggcaatcacg
     4741 ccacctagac tttcaatact agccacttcc tctgaatctg cctgagacag attttcaatt
     4801 gaatggaaat attgaagtaa aagctgacta accttgcttc cgacatgacg aattcccaaa
     4861 ccaaataaga gcttctcggc agaattttcc tttgatgctt ggatagcctg atacagttta
     4921 gcagcggact tttccttaac cccctctaaa aggaggaaat cctcttcttg caaacgataa
     4981 atatccgcca catccttgac taaattagca gcaaaaagct tctcaacaat agatggacca
     5041 aggcctgtaa tattcatagc atcacgagaa gcaaagtgaa tcaagccttc catgatttga
     5101 gcagggcaac gcggattgat acaacgtagg gccacttcat cttcaaagtg caacaagtca
     5161 gagttacaac ttggacagtt tgtagggata tctagttttt cttcagaaac ccgtttggac
     5221 tctaccacac gtaaaacggc agggatgatg tcaccagcct tatatacaat gaccgtatcg
     5281 tcttttcgga tatctttttc agcaatataa tctacattgt gcagggtcgc acggctaaca
     5341 gtcgtaccgg caagttgtac tggtgttaga ttagcagttg gagttacaac accggtacgg
     5401 ccaactgtcc agtcaactga taagagttga gcttcttttt cttcggcagg gaacttgtag
     5461 gctactgccc actttggagc cttaactgta aaaccaagtt cttcttgact tgctaggtcg
     5521 ttgaccttga ttaccactcc atcaatatcg taaggcagat tttcccgttc ctgtcctact
     5581 tcttggataa aattccagat ttcatctatg ttttcagcca agattcgctt aggattgacc
     5641 acaaaaccta gttgttctag gtacttcaaa cccttttctt ggctatcacg agttgaaggg
     5701 ctggcttctt gatagagaaa cgttgcaaga ttacgcttgg caactactgc tgtatccaac
     5761 tgacgcagag ttcctgctgc cgcattacga ggattagcaa attcaggctc tccattttct
     5821 tggcgcactt ggttaacttg gtcaaaggaa gcgcgtggca tgtaacattc cccacgaact
     5881 gtgatatcta gttcttctgg caaagtcaaa gggatgtcct taacacgctt gaggttttct
     5941 gtgatatttt caccaattga accatctcca cgtgttaccc cagcaaccaa aatccccttt
     6001 tcataagtca gcgagataga taagccatcg attttcagct cacaaatata ggtcggatga
     6061 gccacttcct tacgaacacg cgcatcaaaa gcatctagct cctcacgtga aaaagcatcc
     6121 tgcaaactat aaagaggata ctgatgactg tatttttcaa aaccatctaa aaccttgcca
     6181 ccaacacgat gagtcggact gtctgctagc acttgctctg gataagcagt ttctaactcg
     6241 accaactcac ggtaaaggcg gtcatactca ctgtctgcaa ccgagggatt atcgctggta
     6301 tagtactcag tcgcatagcg attgagcaaa gcgactaact cattcattct tttattcata
     6361 agaccatttt accataaaac aagccctcct cacaaacgag aagggcggaa aaaacactta
     6421 gtttgaaatt atttttgaaa ctcaagcaac cttatatcaa tttttcaaaa tgagttcgaa
     6481 cataaataaa cgatatacaa gacaagatga taacaccact tccaattatc aggaaagaag
     6541 agagatgtac acttggcaag actgtcataa atccttttgc aataggcata aatagaatag
     6601 ctaaggtaaa aattgtactc agtactcttc caagaaattc gctctcaacc ttggtttgta
     6661 cttgagtaaa aaagtgaata ttaaaaatcg tcataaacaa ttcacaaact aaatttccag
     6721 aaaaggaaag aaaagttgga agtggtaatc ccatcataaa aactccgaca cctgtcaaag
     6781 ccagtaaaat caaaagatta taaatattag ctttaatttt actagctaga agagccccaa
     6841 tgatggaacc aatagccccc atagttaaaa tacttgcata ggctccttct gacccgtaaa
     6901 gctgattcga aaagggaagt agaaattcaa aagctgcaaa aaagaaatta acgctggaag
     6961 ctaccagcaa aaggaagaaa atttcttgct gatgccagat atagtgtaac ccatccttga
     7021 tatctacaaa aatatctctc ccagtaaaag cctttttctc ttgaactttt gcttcctctt
     7081 ttggaaggaa agccactaga acaaaagcaa tgaaaaaagt cagcgagtct agcagtagcg
     7141 tcatattgag acttgcaaac tgtaaaacaa ggaaggaaag aacaggagag ctaacaccta
     7201 caacctgcaa aaccagctct aagcgagaat tatagatcac aatctcatct ttctccacca
     7261 cttcagttat gatagcttta ttggctgtgc gagaaaaggc aaaagcaata gcctgcacaa
     7321 tgttagcaac aatcaaagcg ccaatcatcc agctatcatt ccttatgaaa gaaatagcca
     7381 gacaaagaat cccacaaaca agatctgccg tcattaaaat cttacgacga gaaaaacggt
     7441 ctgaaataac tccgccaaag ggattgacga gaatagatgt gacgagctca gaaatctgat
     7501 acattcctaa aactgtctgt cctatagtcc ccatagaagc caaccagaca ctatttccat
     7561 aatcatagag catatttccc attttattga tagccccacg gctaatcaac tgcactgcat
     7621 agcgattcat attaaagctc ctctcaaatt ttgaaactat tgtatcaaaa ccgaaaggag
     7681 ctttttattt tttcccttat ttgggaaaat taacttttga caaatttttc gtagtgttcc
     7741 tgataatagg ctacttgctc tggaagacct aacacatcaa aaatatgcat ggccttttgc
     7801 atctgcttac agccttcttt acactgtcct ttttgatata aggcaaaacc ttttaaataa
     7861 tggaaaacat tacgctcata aagcttaata cctttgtcaa taatcttctc tgtataagcc
     7921 tcaaaatagt tggcattata aaaagaagaa tgctctaaac aatgctggta acaattgagg
     7981 gccaaaatca aaactaatct cttatggcga ctaatctctt ggtaaaattc ctccctctcc
     8041 ataacttctc taccaatccg agtgacatag tctacatcgt agaaactata gaggttaccg
     8101 aaaagaatca actcatacat ggtccattct tctgttttga agagataatc tgctacctta
     8161 tccaaatcat cctgcttcat atcataagtc gcatctcttt gacaaatcag accttgtagc
     8221 aaaatccagt tcagctcaaa ataaagggaa gtcgtcgaac tcttagactt ttcaagttgt
     8281 tctctttgaa gcttttgaaa acctgcaata tcgtttgaat agtaaagtgg gataatctgt
     8341 gccatcatag acacatgttc atgattatga aaattccttg ccttatccat gaaattttcg
     8401 attgttacat gaatgttatc caaaatctca aagaaacggg agactgccag gtcagactcc
     8461 ccaagctcaa agcgagataa ctgagaggta gagcaggatt cgcctgctgc ttcctttaaa
     8521 gaataatttc cacttgttcg aaattcacga aatacttttc caagatgttc catctttaca
     8581 cctgatctga taattcttcc cactcaagca tagcttcttc ctgacgatgg ctgattttgt
     8641 ccagctcagc ctgtaattcc atgagtttgt cggcatcgtt tgtttccaac atttgttcag
     8701 aaatggcttg gctttgactt tctagctctt caatttcagc ttctagactt tcgatttgtc
     8761 gcatgagttt gcgaacttct ttttgacttt ctttctgggc ctgatagtca ttgactggac
     8821 ttgcttcctt tgcttgattg ctagttgaag cttcctcagt ctgactcatt tctgctgttg
     8881 ctttcttctc aacatagtag tcgtaatctc caaggtagag agttgaacca ttctcagaca
     8941 attccaaaac atgagttgcc acacgattga taaagtaacg atcatgactg acaaaaagca
     9001 aggtaccatc aaagtcaatc aaggcatttt ctagcacttc cttactatca atatccaagt
     9061 ggttggtcgg ctcatccaga atcaaaaagt tattgttttc catagacaat ttagctaaaa
     9121 gcaaacgagc tttttcgcca ccagatagca tgccgactga ttttttaaca tcatctcctg
     9181 agaaaaggaa ggccccaaga cggttgcgga tttcaacttc tggtgtcagt ttgaaatcat
     9241 tccagagttc atccagcacc gtattacttg gtgtcagctt gctttgggtt tggtcatagt
     9301 aaccaacctc aacattagcg ccaaagcgct tttcaccttt gataaaagga atctggtcca
     9361 caatagactt gataaaggtt gacttgccga taccatttgg accaacgata gcgacagcat
     9421 tcatcttacg aagatctagg ttgataggtt gtgacaaaac ttccccgtca taaccaatag
     9481 cagtattttc aacagtcaaa acaacattgc ccgacgtttt ttcagactgg aaggtcatgt
     9541 tggctgcttt cttgccagct tcaggcttgt ccaaacgctc cattttttct agttgtttac
     9601 ggcgagattg agcacgtttg gtcgttgaag cacgaactag attgcgattg acaaagtctt
     9661 ccagagcagc gatttccttc tgttgctttt catagttttt tgcctcagta gctagctttt
     9721 gctccttcaa ctcaacaaaa cgagagtaat tccccacata gcgatccaag gaatacttgg
     9781 tcaaatctag cgtaattgtc gcaaccttgt ccaagaaata acggtcgtgg ctgacgataa
     9841 tgagggcacc gctatagttt accaagtaat tctctagcca ggagatggtt tcaatatcca
     9901 agtggttagt tggctcgtcc aagaccaaga gattgggctt ttcaaggagc attttagcta
     9961 gagccaaacg agtattttga ccaccagaaa gctcagcaat tttcatctgc cacatagact
    10021 cgtcaaactt gaatccattc aaaatcgctc gaatatcagc ttcataggta aagccacctg
    10081 cttggcgaaa attttcagat aagcggtcat aatctgacat cagtttatcc aaatcctcac
    10141 cagacttttc acccatctcc agctccatct gacgcagttg tttatctgtc cgacgtaagt
    10201 catcaaagac atgaagcatc tcatcataga tggtattttc agactcaaaa cggctatctt
    10261 gagctaggta agacagagaa atatcttttt tcttattgat ttctccacta gttggctcct
    10321 cttctccaac taaaatcttc aaaagagtag acttacctgc accatttttc ccaacaaggg
    10381 caatccgatc tcgttcatca acctgcaggt tgatattatc gaaaagaacc tctcctgcaa
    10441 aagaacgttc aattttatta gcttgtaaaa taatcataca agtagtatag catgtttccc
    10501 taaggcattc aagataatcg taagt
//
LOCUS       DAVMGZ010000063        10511 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000063 DAVMGZ010000000
VERSION     DAVMGZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10511
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.064"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..141
                     /locus_tag="VYQ93_001285"
     CDS             <1..141
                     /locus_tag="VYQ93_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3335971.1"
                     /translation="LEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLS
                     RA"
     gene            complement(220..1413)
                     /locus_tag="VYQ93_001286"
     CDS             complement(220..1413)
                     /locus_tag="VYQ93_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV3335972.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVFCLYIIS
                     LIFTGDMKSILQKMGEHSMLLLFLGYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(1551..2993)
                     /gene="gtfA"
                     /locus_tag="VYQ93_001287"
     CDS             complement(1551..2993)
                     /gene="gtfA"
                     /locus_tag="VYQ93_001287"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV3335973.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            complement(3270..3350)
                     /locus_tag="VYQ93_001288"
                     /pseudo
     CDS             complement(3270..3350)
                     /locus_tag="VYQ93_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3351..4184)
                     /locus_tag="VYQ93_001289"
     CDS             complement(3351..4184)
                     /locus_tag="VYQ93_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3335974.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     VFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(4198..5064)
                     /locus_tag="VYQ93_001290"
     CDS             complement(4198..5064)
                     /locus_tag="VYQ93_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3335975.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVNLFLLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(5078..6337)
                     /locus_tag="VYQ93_001291"
     CDS             complement(5078..6337)
                     /locus_tag="VYQ93_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV3335976.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNAYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTSFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(6429..8591)
                     /locus_tag="VYQ93_001292"
     CDS             complement(6429..8591)
                     /locus_tag="VYQ93_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV3335977.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            8699..9559
                     /locus_tag="VYQ93_001293"
     CDS             8699..9559
                     /locus_tag="VYQ93_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV3335978.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKDKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            9556..10491
                     /locus_tag="VYQ93_001294"
     CDS             9556..10491
                     /locus_tag="VYQ93_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV3335979.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLQQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
BASE COUNT         3407 a         2231 c         1828 g         3045 t
ORIGIN      
        1 ttggaagcga ccaataatct catcaaactt atcaagcgca atgcctttgg ttttcggaac
       61 tttgaaaact tcaaaaaacg gatttttatc gctctgaata tcaaaaaaga aaggacaaaa
      121 tttgtccttt ctcgagctta gctgacttca acccactaca gttgacaaag agcctaaaaa
      181 atctcctacc caaggtagaa gatttcaaac ttataaaact taatccgtca tgtccgatac
      241 caacattcga tgctccaatg gaatactgca cataactagc aagaaaataa agcctgactg
      301 aatccagaag agagccaagt caaaaattcc gtgcacagca accactgtaa ggaaagatag
      361 ataaaggccg ataatcggac gtttccccga ctcctgactc atatccatca tcaagcgaac
      421 aggagcaaca gaagacagaa ctaataaaat ggtacccaca attccgtaac tcagaatcgt
      481 atcaatataa agactgtggg catgttcatg ataaggagca tgtatccgag gataagagtg
      541 catataggtc aatggccctt caccccaaaa aggattttgc ttaaacaagg ccatcccagc
      601 atcccagata gaaatgcgtt cttccataga agagtctaaa gtacccattc gaactcccaa
      661 atcactagaa aagaggaaac tcaaaccaat cgcgaagacc ccaatactaa gccaaaaggc
      721 cttccagttt ttaatagtcg taaagagata gataattgct ccagcgataa tagcaggaaa
      781 ggcagttcga ttttgagtaa agttcaaacc aaagagatta acaaagcctg caatcacaca
      841 gaatactttc aaccaattca acttggtcgt tgtaaacaga tagaaagcaa tcataataca
      901 gaaacaacaa ataattccat aataattagg attaaagaag gtcacttctg cccggttctg
      961 atgccacacc tgcatattgg gggaaagaaa agcataatta aatttcttca caatttggaa
     1021 atgttctaaa ctggcaaaag cagctgacaa gacactacca aacaagacga actgcaaaat
     1081 caatcgaaag aatttatggg ataaaatcga ctgatagtgc aaaaagaaaa tagtaaatag
     1141 aaacattcct actgaagcca caagacccat ccaattttgt gcaagaatgg atataacagt
     1201 actatagcca agaaaaagaa gcagcatcga atgctccccc attttctgaa gaatactttt
     1261 catgtctcct gtaaaaatca gactgataat atataaacag aatacaacta caaaaagata
     1321 aaagggtaaa aagatactca ggataattcc caataaaatc agctctttac tagacaaccc
     1381 cttcagcttt tcaataaagc ctattgattt caaaatgaat cctttctctc caaatcagct
     1441 gattcagata atagtaagct atcctatatt gtaccacttt tttagcaatt tgaaaacaaa
     1501 ggaaacgttt tccaaaataa aaaccctatt ttatccacca tatcaaggct tcaaaatgtt
     1561 acttcaactc cattctcaat tacccgataa gtctgatttt gcaagtcaat ttctgctact
     1621 gctgttacgg acttatcttt attttgacgt ttgattacaa tgctgtgagc tgttggtgtc
     1681 tctatctcag tagtcccttc tagatcaaag gcttctgaac ggttacggaa agaaaataga
     1741 ttgagaaggg ccttcacaac aggtcgttgc acttcttttg ctatttcctc gttgctatag
     1801 taatgacgat taatatttcg accttcttta gtttcttcta ataatttcaa gtcattcttg
     1861 cctgctaata gacccacata gtaaacctga ggaatacctg gggcaaaagc ttgaattaga
     1921 cgagcgagaa aatacttgac atcatcatct ccaagcgctg aatagtaggt tgaattgatt
     1981 tggtagatat ctaagttgtt atactcggca ctagagtact tacgtttgac attggctcca
     2041 accttataga gttcatttga agcatagtca atctcctcat cggtcaggat atccttgaca
     2101 tctactactc caatcccatc atgggtatct agcgtcgtaa attgcttcat cgggctcatc
     2161 tttaaccact tagccaaacg ctctgttctg gaactgtaaa gagtataaag tgtcaccatt
     2221 ggaagagcaa aatcataaac atagtaatca tggtctgcta ttttaaactg aatcgaatag
     2281 tgttcatgaa tctcaggtaa aagctctgtc ccatactcag cagcgatatc tcgaactttg
     2341 tccaataaat cccaaatatc tggttccaca aagaaatcat tagtatccaa tttcttcact
     2401 gcataagcaa aggcatctag acgaatcaaa tcacacccat tacttgccaa gtgctgaatg
     2461 gtcttacgga taaattccat agttacttct ttggtcacat caagatcaat ctgctcctca
     2521 ccaaaggtat tccacaaatg ttccactgaa ccatcttcaa acacaatctc ttgctttggt
     2581 gcacgatcct tacgcttgta aattaaatct acatcagact gtgtcggacg gttttctggc
     2641 caaaacttat cccagtttaa aaagagagct ttaaattcac tggcttcatg tttttcttga
     2701 tagtccttat aatacttgga ttgacgagaa atatgattaa tcataaaatc aaacataaga
     2761 taatatttct cacctaaacg cttaacatcc tcccaatcac caaaagctga gtccacttcg
     2821 tcgtagtcaa ctggcgcaaa tccacgatca cctgttgatg ggaaaaatgg taaaaggtga
     2881 actcctccaa tagcatctcc aaaatgctct tccaaattat catataagtc tttaagatta
     2941 tttccaaggc tatcagaata ggtaatcaac atggttttat tttgaatcgg catcattact
     3001 ctcctttttc taattgaagc caagtctcat atgatctggc ttcgtaaatc aaattcattt
     3061 taaatctcta tttatcatca aactcgtact aatatagact gtgataaaca aagtactact
     3121 ttcttgtttt ctacatagaa ttatcaacaa gctgaactct tcctctgtgt caaagactat
     3181 agattccatg agctcttctt atactcttcg aaaatctctt caaaccacgt cagcttcacc
     3241 ttaccgtagg tatggttact gacttcgtca gtttcatcca caacctcaaa acagtgtttt
     3301 gagcaacctg cggctagctt cctagtttgc tctttgattt tcattgagta ttacttcact
     3361 gccccgttgc tcattcctga aatgatatgg cgttggaaga agagatagac aatggtgata
     3421 ctgataatgc cgaccacgta agaggcaaag cttggtccgt agtcgttgaa atattggcct
     3481 gcgtagttgt attggaacaa aggcagagtc cacattttgg aatcccggtt caagacaagg
     3541 agtggcaaca tgaagtcatt ccagaaccaa agggcattga tgatcatggt tgtcgcatgc
     3601 atcggtttca tcattgggaa gatgatgcgg aaataggttg taaattgatt agccccatcg
     3661 atctctgctg cttcatccag actttctgga atcgagattt tgatatagcc aacatagaga
     3721 aagagggtct gtggaatcgc ataggtcaag tagagcaaga tcaaaccaaa ggtattagcc
     3781 aaaccgagtt tactcatcat aaccgtaatc ggaatcatga tgacttggaa aggtacgaag
     3841 attccgagga ttaagagggt atacatgatg gtaaagactt ttcttttact catattgcga
     3901 gcgatggagt aggctgccat agggataaag atcattactg caagtaaaga caagacagtg
     3961 atgacgacag agttccaata atagcctcca atcccatcag ctaagagacg gctaaagttg
     4021 tcccatgtga agttggttgg aaagccaaag aaattatcta caatatcctt agtgggtttg
     4081 aaggaactaa agagggtagc aaggagcggc actaaaatca gaaccgatcc tagaatcaat
     4141 agaatgtatt tgccaatcag ggcttttctt tcatcttgtt tcatcatgct tctcctctta
     4201 aatttcaaat ttcttagata ctctcaattg gatgatcgaa atcactacaa ttaagaggaa
     4261 caagtttacg gcaatggcat tggcataacc gaattggttg tttttaaagg catagttata
     4321 aaccaagagc ccaagtgagg ttgtggcatt gtttggacca ccaccggtca tggcaaagac
     4381 ttggtcaaag gcagtcagcc caccttttag ggctaggata aagaccatag agacacttgg
     4441 tagcaagtaa ggcaattcaa tgttccagaa aacttgcttg ctagtcgcac catcaatcct
     4501 tgctgcctct gtaatctcag ttggaataga ttgcaaacca gctaggaaga tgatgatggg
     4561 catagccacc ccttgccaaa gaaggacaaa gacagccgca aagattgctc cccacttagt
     4621 ccctaaaaga ctggtttgga gaaattcaat atgaagggca ttcccaatcg ctggaagacc
     4681 gtagttgaag acttgcttga agatcaaagc cactgtcaaa ccagataaaa cagctgggaa
     4741 gaagaaccaa gcacggaaga aggtttggcc tttgatttta gagttcaaga cacgcgcaat
     4801 gaagatcccg agtgcaatct caccaaccac catggcaatc gtaatgattg cggtaaagcc
     4861 aatcgcattc atgaattttg gatccatgaa gaggagctta aagttgttta agccgacaaa
     4921 tttgtagttg taagtcaatc ctgtccagtt ggtaaaactg taaaaggctc cttgaaacat
     4981 cggcacatag aagaaaattg cttgtaacaa gagggggatg accacaaaag cccatgccca
     5041 atatttttgt aatacttttt tcatagtctc tctactccta atccacatcc gctttcatcg
     5101 ggttaaagaa ggcattcaaa tcattgacca tgccttgttt atcaccggtc aagacatagt
     5161 tcatggtcaa ggtatggaag tctgcttcac tggtccagta ttgttgcaac cagaccaagt
     5221 gacgatccgt aaaggcatat tcggtcatac cagcaagcgg tgaatcttct cctgcttgtt
     5281 tgaccccttc gatcgctgtt ggagatccgt ccacatcgta gtatttttgc atgacttctg
     5341 gacgggtcat atattccaca aaggcattgg cttcttttgg atgtttggtg gtggctgaga
     5401 tagaccatgc caagtctccc gcaccaacgg ttaagctttg tcctttttct tttcctggaa
     5461 tcatgaaggt tccaatctta aagttcggtt tttgttcatt aatcgctgtg atcgcccaag
     5521 acccatttgg tgtcatgagg acatccccac gtgcgaaggc tccgataaca tcggtatagc
     5581 cagcaccttc ccagttcttt tgcttagagc cattgatgcg aaggatgtcc atgaccttga
     5641 tatcatcttt cataatcgga tccgacaatt taatggcatt tggttgagaa taacgaaggt
     5701 attgatttgc ttcttttcct ccacctgttg ctgtcgcaaa ggctaattga ttgtaaccat
     5761 tgagtgtcca agcatctgca cctgcaattc caaatgatgt ttgtccttta gcaacgatat
     5821 ctttgactaa ctgttcaaat tcatcccagg tttcaggaac cttcaagccc agttcttcga
     5881 atttatcttt gttgtagtaa attccataag cattagctgt aaaaggaacg ttataaactt
     5941 tttcgtttac agcatatttt tcagcgtagg catttttcac gcgtttcagg tagtctttgt
     6001 tgctcaaatc ttcaaaaaca cctgcttttg cccattcttg cagttcgatg gactgtgggt
     6061 aaatattgac cacatcaggc acatctcctg cgagaacgcg tgtcttcaat acttcaccag
     6121 catttggtac attgacgact ttgaccttga tcttagggtt ttccttctca aaatcacgag
     6181 tgatttcttc caaggttttg gtcatttctt ttttctggtt gaaatactcg atggtcactg
     6241 tgccatccgc agatttacca tagttggagc aagcgccgag cccaaacaaa gctaaacctg
     6301 tagttgcaag aagtccgatt tttttatacc attccattag aaagcctcct ttataaattt
     6361 atacaccctt attgaactgc accccaaaag ttagacagaa taaatctaac ttttggggtc
     6421 agtacatatc atagttttct aaaaatatac tgtctactca aaaaatctcc ttgggataag
     6481 ataacagtta agcccgcata cattagttct gcacctgagt aaacttcgcc attttcctgt
     6541 aatttatata gtccctcttc atccaaatct tttaatttta aagttgtttc catggtctct
     6601 acaacagata aaacgcgaac gtaggttaca atcgtttgat ttccgtaatt aaattgtaca
     6661 gctgcttcat tggatgcagt atcaggatta attagtctat actgctgtcc taactgaact
     6721 actggtcgta attctttata caagttcacc tgattagcaa tcgtagcttt ctcttcatct
     6781 gataaatttg tcaaatcaag ctcatagccc aaatttccca tcattgctac atggccacgt
     6841 gtttctaatg gtgtcattcg tcccatctga tgattcggta ctgctgacac atgagccccc
     6901 atagaaatgg ttggatagag ataggatgaa ccgtattgaa ttggtaaacg tgcaatggca
     6961 tcagtattat cactagccca gacttgtggg aaatagcgta tcataccaag atcatttcgt
     7021 ccaccaccac cagagcagga ctcaaagaga atatggctgt gcttctctgt cagataagaa
     7081 acgagttcgt aaagccccag catgtactga tgagattgca tctgtgtctc tagataagtt
     7141 aatccattcc ctagcttagt gatattgcgg ttcatatccc atttaatgta atcaatctca
     7201 tgataagata ggagttgatc taagacactt ttcaagtatt ctactacctg aggattggca
     7261 agattaagta ctaattgatt ccgagaataa gtatgctcat aatcaggaac ctgaatagcc
     7321 cagtcaggat gttgacgata caaatcacta tctacagaaa tcatttcggg ttctaaccaa
     7381 agtccaaact gcaaacctct ttcatggata gctgaaatca gactttctag acttccaccc
     7441 agtttttcct cattaacaac ccaatcacct aaagcacgat tatcatcaaa acgattgcca
     7501 aaccaaccat catctaatac aaaaagttca atgccaactt tcttagcttc atctgctaac
     7561 tctaacagtt tttctctctg aaagtcaaag taagtagctt cccagttatt gattagaatt
     7621 ggacgttctt ttttagaaaa ttcacttagc ataatgtgct tcagtacaaa attctgactt
     7681 tcatgactaa taccagttaa tccctgatct gaatgagtca ctaaagctac cggtgtttca
     7741 aagtattcct caggggctaa cttccaagaa aagttttctg gattaatgcc aatagccacc
     7801 cgaacttcat tcaattgatt tttttgaaca aaagcttcaa agttgccact atacattagt
     7861 tgaatagcaa acacattccc agcatcctct gtgactcctt gttcgcatag tagaagagct
     7921 ggtgtttgag catgaccaga agcacctcgg tttgaactaa tcgaaaagat tccttgttct
     7981 acctgttgac gtctaacagt cttttcacga gcataagcac cctgcagagt tgctatttca
     8041 taatctgcag ctggaaaatc agccataaaa gaaaaatctt tatggatgac aacttcctga
     8101 ttactattat tatctaattt actgtagcta gtaatagtcg catcattatt aaaagtagta
     8161 taatacaaag tcagactaag ttgagcctta gaatcttcta acattaagac aagagtctct
     8221 gtatcgtcca tgctatgtgg agaaggtaag ccctgtggac cattctgacc ttttaaaatc
     8281 tttgcttcta caaatcgaaa gtctgttact tcagttacac tatgctgaac ctgtatagtt
     8341 ggtttcctaa aatctcctaa gccatgttgt ccaaaaatct gtcgctgagt atctaaacta
     8401 aaggttcgat tagtagccgt tggatttcct gaaaaggcat ggtctcgttc ataaacacta
     8461 ttggaacctt tatagttctt aatagtcttt cctaaatgtt tcaaaagtaa gtagccattt
     8521 cgattttcaa taatcaaact tagattttta ctctcaacat aaaatagatt attctctatc
     8581 ctaactccca tttacttcac ctcatcactt tattgattat attttatcac ctgaaatcgc
     8641 tttccaaaat agaaaaatgt ctcaagaata tggtaaaatg ttaggtagga ggtagcacat
     8701 gttagttttt tcagaatacc agactggaac aatcgacctt gccctaagct tttatggata
     8761 tgaggaatgc acacctaatt actcttttgg tccagccatt cgtgatacat acgtcctaca
     8821 ttacattact aaaggacaag gaaaatttca ttacaaggat aaaattgttg atttaaaaga
     8881 aggagatttc tttctattaa aaccagagga actaaccttt tatcaagcag atagtaaaga
     8941 accttgggcc tactactggt taggaatcac tggagggaaa tcccctgatt attttgctct
     9001 ttctcaaatt tctgatcaat cctatctcat ccaatctgaa acttgtcata cccagactac
     9061 tgcaaaactc atctcagaca ttgtccgctt cgctcagatt acaaaatcaa gtgaattagc
     9121 tcaactccat atcatgggac aacttcatga actgatgttt catctgggaa ctattgctcc
     9181 caatcagaaa aaaaagaata tttcatcaac ccaccaactc tatcttgaat gcaaacgatt
     9241 aattgatagc cactatcctc aatcacttac aattcaagat ttagcaaaag aactatccgt
     9301 tcacagaagc tacttatcaa gcgtattcaa agaatttaat accttatcac ccaaagaata
     9361 cctactctac gttcgaatgc accgagctag acaacttctc gaaaataccc aagagtccat
     9421 caaggtaatt gcatactcgg taggtttttc agatccactc catttttcga aagcttataa
     9481 acaatacttt aatcagactc caagtcatac aagaaaagaa tactctcaat accaactagt
     9541 gagaaaggca acattatgaa atcctaccaa gctgtctacc aaatcctatc taaagaaacc
     9601 gactatatca gcggagaaaa aatcgcagaa aaactatccc taagccgaac atcaatttgg
     9661 aaagctatca agcgactaga acaagaaggc attgaaattg atagtatcaa aaatagagga
     9721 tataaactaa tgaatggtga ccttattctt ccagagattc tagaagaaaa tcttccaatt
     9781 aaagtcagct ttaaacccga aacaaaatca acacaactag atgcaaaaga agcaattgat
     9841 ttaggccatg aagcaaatac cctctatcta gcttcctatc aaacagcagg ccgaggacgt
     9901 tttcaacgtt ccttctactc accacaaggt ggtatttata tgacactcca tcttaaacca
     9961 aatctcccct atgacaaatt accatcctac acactacttg tagctggagc tgtctacaaa
    10021 gctattaaga acctaacttt aatagatgtc gacataaaat gggtcaatga tatctatcta
    10081 aacaatcata aaattggagg aatccttact gaagcaatga cctctgtaga aactggctta
    10141 gtcacagata tcattattgg agtaggtatc aatttcacta ttaaagactt ccctcaggaa
    10201 ttaaaagaaa aagctgccag cttatttaaa gctacagctc ctataacaag gaatgaattg
    10261 atcatagaaa tctggcgtgc tttcttcgaa acaccagcag aagagctatt atacctatac
    10321 aaaaaacagt cattcattct aggaaaagaa gtcactttca cactacagca aaaagactac
    10381 aagggacttg ctaaagacat ctcagaaaat ggaaaacttt tagttcaatg tgataacgga
    10441 aaagaaatct ggctaaatag tggcgaaatt tctctcaata gttggaagta aaataacaca
    10501 attataatat a
//
LOCUS       DAVMGZ010000064        10380 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000064 DAVMGZ010000000
VERSION     DAVMGZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10380
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.065"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..389
                     /locus_tag="VYQ93_001295"
                     /pseudo
     CDS             1..389
                     /locus_tag="VYQ93_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            475..687
                     /locus_tag="VYQ93_001296"
                     /pseudo
     CDS             475..687
                     /locus_tag="VYQ93_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            587..1033
                     /locus_tag="VYQ93_001297"
     CDS             587..1033
                     /locus_tag="VYQ93_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3335980.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1171..2475)
                     /gene="eno"
                     /locus_tag="VYQ93_001298"
     CDS             complement(1171..2475)
                     /gene="eno"
                     /locus_tag="VYQ93_001298"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV3335981.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2639..3085
                     /locus_tag="VYQ93_001299"
     CDS             2639..3085
                     /locus_tag="VYQ93_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV3335982.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3082..3885)
                     /locus_tag="VYQ93_001300"
     CDS             complement(3082..3885)
                     /locus_tag="VYQ93_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV3335983.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3894..4223)
                     /locus_tag="VYQ93_001301"
     CDS             complement(3894..4223)
                     /locus_tag="VYQ93_001301"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV3335984.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4168..4539)
                     /locus_tag="VYQ93_001302"
     CDS             complement(4168..4539)
                     /locus_tag="VYQ93_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV3335985.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4598..6031)
                     /gene="glgA"
                     /locus_tag="VYQ93_001303"
     CDS             complement(4598..6031)
                     /gene="glgA"
                     /locus_tag="VYQ93_001303"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV3335986.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6028..7167)
                     /gene="glgD"
                     /locus_tag="VYQ93_001304"
     CDS             complement(6028..7167)
                     /gene="glgD"
                     /locus_tag="VYQ93_001304"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV3335987.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7157..8299)
                     /locus_tag="VYQ93_001305"
     CDS             complement(7157..8299)
                     /locus_tag="VYQ93_001305"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV3335988.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8289..10217)
                     /gene="glgB"
                     /locus_tag="VYQ93_001306"
     CDS             complement(8289..10217)
                     /gene="glgB"
                     /locus_tag="VYQ93_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV3335989.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELREKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3180 a         2284 c         1826 g         3090 t
ORIGIN      
        1 caccaagtaa aaggaacaaa accaagaaaa gttgatagag atagacttaa aaactatctt
       61 actgacaatc cagacgctta tttgactgaa atagcttctg aatttggctg tcatccaact
      121 accatccact atgcgctcaa agctatgggc tacactcgaa aaaaaagaac cacacctact
      181 atgaacaaga cccagaaaaa gtagccttat ttcttaagaa ttttaatagt ttaaagcacc
      241 tagcacaggt ctaacaaatg aatggtgagt taatcgctcc aatgacttac gaagagacga
      301 tgacgagcga cttttttgaa gcttggtttc agaaatttct cttaccaaca ttaaccgcac
      361 cattggttat tatattaaaa cgaagtaaga ataggacaaa tcgatcagga cagtaaaatc
      421 taattctaac aatgttttag aagtagaggt gtactattct agtttcaatc tactatatta
      481 atggacaatg caagattcca tagaatgggg aagctagaac tcttgtgtga agagtttggg
      541 cataaacttt tacctcttcc tccctactcg cctgagtaca atcttattga gaaaacatgg
      601 gctcatatca aaaagcacct caaaagggta ttaccaagtt gcaatacctt ttatgaggct
      661 cttttgtctt gttcttgttt caattgacta tataaattgt ctaagcgaaa caaccgataa
      721 gaattggcac aaaagcgacc gtatttttgt taccaataca ggaaaaccag ttcatagttc
      781 tatcttgagc aagtctctcc agcgagcaaa cgaacgcctt aaaaaaccaa ttcccaaaca
      841 tctgtcccct cacatcttca gacacaccac tattagcatc ttatcagaaa ataaaattcc
      901 tttaaaaaca atcacggaca gggttggtca ttccgactct gaagtcacta cttccatcta
      961 cacccacgtc acaaagaaca tgaaagatga agcaatcaat gtactggata aagttatgaa
     1021 aaagattttt taaaaagttt tgtccctttt ttgtcctcta aatacaaaaa tagcccttcg
     1081 gataaaatcc gaggggctag aaacgttgtt aaatcaacgg ccgaactttt gaatttcatg
     1141 gttcgggata aaatagttca ctgaactatt ttatttttta aggttgtaga atgatttcaa
     1201 tccacgatat tcagctactt caccaagttg gtcttcgata cgaagcaatt ggttgtattt
     1261 agcgatgcgg tctgtacgtg aaagtgaacc agtcttgatt tgtcctgcgt tagttgcaac
     1321 tgcaatatca gcgattgttg aatcttcagt ttcacctgaa cggtgtgata caacagcagt
     1381 gtaaccagct tctttagcca tttcgatagc ttcaaaagtt tcagtaagag taccgatttg
     1441 gttaactttg ataaggattg agttagcagc accttcttgg ataccacgtg caaggtagtc
     1501 agtgtttgtt acgaagaagt cgtcaccaac aagttgtact ttcttaccaa gacgttcagt
     1561 aagagctttc caaccatccc agtcgttttc atccatacca tcttcaatag tgatgattgg
     1621 gtatttgtta accaattctt caaggtagtc gatttgttct gcagatgtac gaacagcagc
     1681 accttcacct tcaaatttag tgtagtcgta aactttacgt tctttatcgt agaattctga
     1741 tgaagcacag tcaaatccga taaatacgtc tttacctggt acatatccag cagcttcaat
     1801 cgcagcaagg atagtttcaa caccatcttc agttccttcg aaacgaggag cgaatccacc
     1861 ttcgtcacct acggcagttt ccaaaccacg tgatttaagg attttcttaa gagcgtggaa
     1921 gatttcagca ccgtaacgaa gggcttcttt aaatgttggc gcaccaactg gcaagatcat
     1981 gaactcttgg aaagcgattg gagcgtcaga gtgagaacca ccgttgatga tgttcatcat
     2041 tggagttgga agaactttag tgttgaatcc accaaggtag ctgtaaagtg ggatttcaag
     2101 gtagtcagca gcagcacgag ctacagcgat agacacaccg aggattgcat tcgcacccaa
     2161 tttacctttg ttaggagtac cgtcaagtgc aatcatagca cggtcaatag cttgttgatc
     2221 acgtacatcg tagccaatga tagcttcagc aatgatgttg tttacgttgt caacagcttt
     2281 ttgtgtacca agaccaccgt aacgagattt gtcaccgtcg cgaagttcaa ctgcttcgtg
     2341 ttcaccagta gaagctcctg atggaaccat accacgtccg aaagcacctg attcagtgta
     2401 aacttctact tcaagtgttg ggttaccgcg tgagtctagg acttcgcgag cgtaaacatc
     2461 agtaataatt gacatttttt actctcctta tgagttaaat tttttacacc tctataatac
     2521 cttaaaaccc ctcctttttc aagaaaaaac gttatctttg tgcaactttt ccttaacttt
     2581 ataaagtaat cgctttcttt tgtctgtttt attctaactt ttatgatata ctgttttcat
     2641 gacagattta tcaaaacaat tacttgaaaa agctcatggt gggttaaaaa taaatccgga
     2701 tgagcaaaga cgctatcttg gtacttttga ggaaagagtt cttggatatg tagatattga
     2761 cacagcaaat agccctcagt tagaaaaagg ctttttattt attttagaaa accttcagga
     2821 aaaagcaaag ccactatttg tgaagatttc accaactatc gaatttgata agcaagtttt
     2881 ctacttaaaa gaagcaaaag aaactgatag tcaagccacc atagtatctg aagagcatat
     2941 tacttctcct tttggcctgg ttattcatag caatgcacca gttcaagtag aagaaaaaga
     3001 ccttcgactt gcttttccaa aactttggga agttaaaaag gaagaaccag ccaaaacatc
     3061 cttatggaag aaatggttta gctaaatctt gcacatattt aataagtgcc caatattggc
     3121 agccgtgcgc tccagataga gactggcatt tttcaaacta tcttctaaag gttcactttt
     3181 ctccaaaata gaaaagacag cttggatatt ttcaaatggt aggggaggta aatcttcagc
     3241 aagactaccg caaatagcaa taacaggaac tccaacaggg gttctttttg caacacctat
     3301 aggcgctttc ccagcaaagc tttgactatc aagtcttcct tctccaacaa caaccaagtc
     3361 agcatctgaa actttcttat caaagttgat taagtccaag caggtatcaa ttccagacac
     3421 gatacttgcc tgagcaaagg cacacaaacc agcagcaatc cccagtcctc catcattact
     3481 ggccgtgcca ccaacaccga tataaatatc tttaatccct ttagaaatga gatggagaat
     3541 caactctcca ataccacaag tttggatttg aagtggattt cgtttctcta gcggaatttt
     3601 tccaagacca accaagtcag ctacttcaaa tagtgccagt tccccttttt gaaaatagcg
     3661 catggcttct ttttgtccaa aagggtctgt cacttggatc catttttctt ttaggtcaag
     3721 agaatgtcgg atagcatcta cagtaccttc tcccccatca ccaacagggc agaggagaca
     3781 ttctacatct gctatcgatt gttggaagcc tctttttatt gcttcagcta cctgttgagc
     3841 tgtcaagctt tccttaaacg aatccggtgc aattacaatc ttcatatttt ccctcattct
     3901 aaacagtcaa tcaaaggaag aacttctaaa aaatccctct tgtcaacatg atgtggtatt
     3961 tcttttttga gcatttcttt ggaacaaaag gcgattccta actccgccga cttcaacatt
     4021 aatagattat taaccccatc accgattgcc accgttcttt ctttagaaag ttttagtttc
     4081 tttctccatt tttccagagt ttcttttttg acctggggac ttataatttg tccaactaat
     4141 tttcctgtta aaagaccttc tttgacttca agctggttgg cagtgaaata ggcaatacca
     4201 agggattttg ctaatctctc caactattgg tgtaaatcca ccagacacca gaccaactag
     4261 gatgccattc ttttggagaa tagagatgaa ctctgggaca tttagcgata gatgaattga
     4321 gttgaagacg ttatcaaaga ccaaaatagg aagaccttcc aacaaggaca ctctttttct
     4381 taaactgctt tcaaagacca actctcctcg cattgctcga cttgtaatct gcgaaatttc
     4441 cgcctcatga cctgcctctc tccctaaaag atcaatcact tcttctagga ttaaggttcc
     4501 atctacatcc aaaacacaca agccttttac ttgagacatc agttctcctc tctaaacagc
     4561 ctaaaaatcg tatgaagtca tcatacgatt ttatctatta attaactaaa ctatggtaca
     4621 agtcaaggta tgacttgcag gctgtatccc atgagaagtc actctccata gcttgttttt
     4681 gtaggtttct ccaaatgtct ggatggtttc tatacaagtc caatgctgtt tggaaagtcc
     4741 aatttaacca ataaggagat agattgtcaa agctaaagcc agtaccgctt ccttcgattg
     4801 gattgaaagc gcgaactgta tctcgcaagc ctccaacttc atggaccaat ggcaaggttc
     4861 cataacgcat agccatcatt tgagacaagc cacacggttc aaaacgactt ggcatgagga
     4921 agaggtcaca agcagcgtag atttcctgag caagtttgac atcaaaagtg atatttgctg
     4981 atagcttgtc tgggtaaatc tgagcaaacc atgagaaagc tccttcaaag gctggatcgc
     5041 cagttcccaa aagaacaatc tgaacatctt cttgcaagat atggtgaaga ctttcgacca
     5101 ccacatcaaa acctttttga cgtgtcaaac gagaaacaat tcccaccagt ggaacgtctg
     5161 ctctaacagg caagccaact ctttcttgca attttgcctt atttttggct ttcccagaca
     5221 aatcttcctg attgaaatga tagtctaaaa gagcatccgt ctgaggatta taaagatcag
     5281 catcaatccc attcacgata ccagatactt taccagactc cattttaaga atctgatcca
     5341 aattacatcc aaactgacta gtcataattt catgagcata gctaggtgaa acggttgaaa
     5401 cacggttcgc atagagaata cctgccttca tccagttcag acagttgttc catcgaaggg
     5461 tgccatcagc gtaacgttca aggccaactc caaacaaatc acccaacatt ccttctgaaa
     5521 attgtccttg gaattctaaa ttatgaatgg ttaaaactgt ttcaatgtcc tcataggctt
     5581 gaatccaaca gtatttttcc ttcaacaaga aaggaatcat agctgtatgg tagtcatgaa
     5641 catggagaag atcaggaata aagtcaatcc tttccatagc ctcaatggca gccagttgga
     5701 aaaaggcaaa gcgttctccg tcatcaaaat caccgtaaac atgaccacgg aagaaataat
     5761 attgattgtc aataaagtag aaggttacac catttaatac tgttttctta attccacaat
     5821 actgtctacg ccaaccaacg ctcacctcaa aatgaagcac atcttcaatc tgatttccaa
     5881 atttagcctc taccatatca tagtagggta aaatcactgc aacttcgtgc ccagctttta
     5941 ccagtgattt tggaagagcg ccaatgacgt ctcccaaacc acctgttttt gaaaagggtg
     6001 caccctctgc tgctacaaat aaaattttca tgaatgaata tcctctgtta ctttagcacc
     6061 tttcttaacc acaactggat gttctgcagt tcctcgaatc acaacaccaa gctcaacttc
     6121 aacccctttg tccaagatag catattcgac ctgagcccct tctccaataa caacacgagg
     6181 gaagagcagg ctatctttaa ccaagctatc cttatggaca tgaatattac gtgatagaac
     6241 agaattagct acttgacctt caataatact accagaggca aactgagaag tgcttacctt
     6301 agatgtatta gcatagtaag ttggctcttc gtttttgacc tttgtataaa tcttttggtt
     6361 tggtgagaaa agagaataga atttttgtga ttcaagcata tcgatattcg cttgataata
     6421 agatttaaca gagtgaatat tggctagata gcccgtgtac tcgtaggcaa aagctccctc
     6481 ttttacagcc aaatcccgta aaacatagcg caatttctct ggatgttctt ttttagcttc
     6541 ttcttccaag tgttcaatca accaaggtgt atcaacgaca aagatatctg tagacatatt
     6601 gaacgtttca gctgttgact tgctatcaaa gagtttatga gaaagaacat ggtctgtttc
     6661 atctacatcc aagattgcat ttacttctga aatatctttc ttagctagtt ttttataaac
     6721 tacagtgata ggctcttttg ttgtactatg taggtggaaa acttggttca aatcaatgtt
     6781 aataagaaca tcgcagttga gggcaaccgt ttggtttgag ccagaacgtt tcaaataagt
     6841 aagaagctgt tggtagtatt cttttccaac tgtactactt tctacacggg tattgtaaat
     6901 tcctagatag taatggctaa gaagggttga taagccccac tcgcgccctg aacgaatatg
     6961 gtcaaagact gagctgatat tatcctgctg gaaaatacca aagacactac gaacacctgc
     7021 attagcaagg cttgaaagtg ggaagtcaat caaacgatat ttcccaccaa atggcaaact
     7081 tgctactgga cggtggtccg tcaatgtcga catatcgtga aaaccaactg tatttcctaa
     7141 aatggcagaa tatttatcaa tcttcatctg ttgctacccc cactacttca ttatatccta
     7201 caacttgtac ttcatctgtt ccatcaattt cgacaccgtc agaaataatc gcaccttcac
     7261 caataatggc acgtttaatc ttagctcctt gaccaatgat agctccactc atgataactg
     7321 aatcaaggac ttccgctcct tcgcgaactt gcgcgcctgt tgaaaggata gaatgtttaa
     7381 cagttccatc aacgaaacat ccgtctacaa ctaatgagtc ttccacatga gcatttgccc
     7441 cgaggaagtt tggtggtgaa atcaagtttc ttgagtaaat cttccattga cggttacgac
     7501 tatccaaggc attttctgga gaaatatact ccatgttcgc ttcccaaagt gactcaatag
     7561 taccaacatc tttccaataa ccactaaatt cataagcata aacactttca cctgactcaa
     7621 ggtaatttgg aatgacattt ttaccaaagt ctgacatgcc aaccttgctc ttttcagcag
     7681 cgactaacat attacgaagg cgttgccaat caaaaatgta gattcccata gaagcttttg
     7741 tagatttagg ttgagctggt ttttcttcaa attcaacaat acgattgtta gcatctgtgt
     7801 tcatgatacc aaaacggctt gcttctttaa gagggacgtc taaaactgct actgtcaagc
     7861 tggcattatt atccttatga gactggagca tatcatcata gtccattttg tagatgtgat
     7921 ccccagacaa aatcaagaca tactcaggat tgacactgtc gatatagtcg atattttggt
     7981 aaatagcgtg actagtcccc tcaaaccaac gatttccttc acttgcagaa taaggttgaa
     8041 gaatagagac acctgaatta ataccgtcta gtccccagct tgaaccattc ccaatatggt
     8101 tgttgagagc aagtggttga tactgtgtaa cgaccccaac attatgaatc cctgagttgg
     8161 cacagtttga tagggcaaag tcaatgatac ggtagcgccc accaaattgc acagctggtt
     8221 ttgcgatgct ttgagtgagt ttaccgagac gagttccttg cccaccagca agaatcaaag
     8281 ctaacatttc atttttcatt ttctactcct ttttggtttt tatttgtgac ggttttagta
     8341 gatttcaagc gacgtttgat tttccataca cttgctccca tagccggtag ggtaaaggtt
     8401 aaggtctgct cataatcttt ccatagtcct tcttgcgttt gaacagtttg attatgttct
     8461 ttccaaacgc ctccccactc ttccaactca gtattccata cttcttcgta aattcctgca
     8521 acgggtagtc cgattgtaaa atctttccgc tcaacaggta ccatgttaaa gatacagact
     8581 aacatttctc cctttttacc cttacgaata aaggaaagaa cactctggtc tcgattatcc
     8641 gcatcaatga tttcaatacc atcatagctg gtatcgattt cccacagaca gcgatgatct
     8701 ttgtaaaact ggtttagctg agaagtgaaa tacttcatct tagcattcat tgggtcttct
     8761 aggttagacc attccaactg ttcttcagat ttccattcta ggaattgacc gtattcgcta
     8821 cccatgaaga gcaatttctt accaggatga caaatttggt acgtatagag attgcgcaag
     8881 cctgcgaatt gattgtaacg atctccccac atcttatgca tcatactctt cttgccatga
     8941 accacttcat cgtgcgagaa tggcaagaga taattctcct tgaaaacata cataaagctg
     9001 aaagtcacca ggttaaagtc atatttacga tagatcggat cttcttcgta gaaacggagg
     9061 atatcattca tccagcccat gttccatttg tagtcaaatc ctagaccacc aatctctttc
     9121 attcccgtaa tcttggtcgc agacgaactt tcttctgcaa tcatcatcac atctggatat
     9181 tctaacttaa taacctcatt caagcgctga aggaaataat aaccttcata gttgagattt
     9241 ccgccatctt tattaggtgt ccatggagca tcatcatagt ccaaatagag catgttgcta
     9301 acagcatcca cacgaatacc atccaaatga tagacatcaa tccaatgctt aatgcaagaa
     9361 attaagaagg actggacttc attttttcca aggtcaaaat taagggcacc ccaaccatgg
     9421 ttatgagcct tattatggtc ttggtattca aaagtcggtg tcccatcata ataggctaag
     9481 gcatcatcgt tgatggtaaa gtgacctggt acccagtcca caataacccc aatattatgg
     9541 gtatgacact cctcgacaaa atcttgaaac tcctctggtc ggccataagc atgctctaaa
     9601 gcgaagtaac ccataagctg atacccccaa ctcaagccca aaggatggga catcaagggc
     9661 ataaactcaa tatgagtata gttcatttca acgagataag gaatgagttc atccttgagc
     9721 tgggcaaaac tataaggact gccatcagaa tttcttttcc atgatccagc gtgaacttca
     9781 taaatattga caggacgctc ttcaaagccc caacgttttc ttcgtgccag ccaaagtcca
     9841 tccttccatt tcttctcacg aagctctgtt aagattgccc ctgttcctgg acgagcctca
     9901 tacctgacag caaaagggtc aatcttcatc agttgatgac cattttgacg tgtgacatga
     9961 tatttgtaaa tatgcccttc ttgagccata ttggtaaaga cttcccagac cccaaaatca
    10021 tttcttacca ttggaatctg attttcaatc cagttggtaa aatcaccaac caagtgaaca
    10081 gcctgagcat taggtgccca aacacggaag gtatagccat gctctccatt tagttcttcc
    10141 ctatgtgctc ctagataatg ttggagataa aaattttcac ccgtcataaa ggtttttaat
    10201 gcttctctat tatccatata ctcctcttct cctgtaagcg ttttctatgt ttttattata
    10261 ctaccttttt agagaagatt caagtaaatt actatacttc tttaattatt ttgaaaatct
    10321 acaacaagtt cacttactcg ttcaattgta aatcaatatt ttttcaaaaa attgcgaaaa
//
LOCUS       DAVMGZ010000065        10345 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000065 DAVMGZ010000000
VERSION     DAVMGZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10345
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.066"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(37..109)
                     /locus_tag="VYQ93_001307"
     tRNA            complement(37..109)
                     /locus_tag="VYQ93_001307"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74..76),aa:Lys,seq:ctt)
     gene            complement(374..1645)
                     /locus_tag="VYQ93_001308"
     CDS             complement(374..1645)
                     /locus_tag="VYQ93_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV3335990.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            1943..2158
                     /locus_tag="VYQ93_001309"
     CDS             1943..2158
                     /locus_tag="VYQ93_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335991.1"
                     /translation="MMELPSQEILIFTKQIRHWILSDQVISGKRKLFFREDTPKEILD
                     LYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            2148..2396
                     /locus_tag="VYQ93_001310"
     CDS             2148..2396
                     /locus_tag="VYQ93_001310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3335992.1"
                     /translation="MKNRIIDVFEVVNRLLVITVENPDFEDLRVNQFVKIGDKKYRVR
                     SVPMIHSTPPQSVLDRDTFTIDYTDDELLDKEAVFTTH"
     gene            2533..2712
                     /locus_tag="VYQ93_001311"
     CDS             2533..2712
                     /locus_tag="VYQ93_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV3335993.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGE
                     LNKYTERGIRKQAGL"
     gene            2749..3201
                     /locus_tag="VYQ93_001312"
     CDS             2749..3201
                     /locus_tag="VYQ93_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV3335994.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            3492..3962
                     /locus_tag="VYQ93_001313"
     CDS             3492..3962
                     /locus_tag="VYQ93_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV3335995.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            3981..5069
                     /locus_tag="VYQ93_001314"
     CDS             3981..5069
                     /locus_tag="VYQ93_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV3335996.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLMLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYCENLLAELEKEMLEGTEDGTRNF"
     gene            5050..5478
                     /locus_tag="VYQ93_001315"
     CDS             5050..5478
                     /locus_tag="VYQ93_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3335997.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYKLMPIEEFLNSKQAVPQLQGRLRDYLEESL"
     gene            5475..5948
                     /locus_tag="VYQ93_001316"
     CDS             5475..5948
                     /locus_tag="VYQ93_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3335998.1"
                     /translation="MITLVRAGAEDLETIIAIQRASFKAVYEKYHDEYDPYLEDCERI
                     KWKLVERPNSYYYFVKEDEENIGFLRVQTNAELTKAWLGTAAILPQYQGKGYGSKGLS
                     LLEKEFPTITQWDLCTVLQDAGMVAFYEKNGYRQIHIEAEKEGMDMVYMKKLIEK"
     gene            6086..7036
                     /locus_tag="VYQ93_001317"
     CDS             6086..7036
                     /locus_tag="VYQ93_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV3335999.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPETVDVAAVEADLQARLAELTDFMDLGEVKMGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            7038..7781
                     /locus_tag="VYQ93_001318"
     CDS             7038..7781
                     /locus_tag="VYQ93_001318"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV3336000.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVSGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            7792..9588
                     /gene="pepF"
                     /locus_tag="VYQ93_001319"
     CDS             7792..9588
                     /gene="pepF"
                     /locus_tag="VYQ93_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV3336001.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(9648..10325)
                     /locus_tag="VYQ93_001320"
     CDS             complement(9648..10325)
                     /locus_tag="VYQ93_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3336002.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEKGKEF
                     LEKEWGI"
BASE COUNT         3019 a         1900 c         2485 g         2941 t
ORIGIN      
        1 gccctatatt ataagacttt tcctgttgta tttaaatgcc ccctacaggg atcgaacctg
       61 tgacccacgg attaagagtc cgctgctctg ccagctgagc taaggaggca aagaaaaaag
      121 ctgtattggt gccgaaactt cacggtttgt attgaacccg cgcaattaag caggtgggca
      181 actcgctcta actgaagctg tttccgtgtg agacggccta catgctgtta gaagactttt
      241 gtttccctaa taatacaaaa aatagtcggt caacacttaa gtgtgaagtc gtacaccaca
      301 gcgtttctat atttatatga taccactttt tcaaaaaaaa tcaagaggaa agtgcaattt
      361 tttgaaaagg atttcagatt tttcgcaaat ggtcgatagc ttcctttctt tgagccaaag
      421 cccgttcata tttaccagta tcttctgctt ggaaatagtg atgattacga attttttctg
      481 gcagatagtc ttgctttacc caatttccag gatagttgtg tggatagaga tagtcttggg
      541 cattccccag ttccttgctt ccactgtagt gcccatcacg taggtgtcgc ggaataggca
      601 agtgccctga tgttttgagg tcagcaagtg ccttatccat agctacatag gctgagttgg
      661 attttggaga aagggccaaa tcaatcacga cattggcaat gagaatgcgg gcttctggga
      721 acccaatctt ctgggcagca tccagagcag tcacggtatg aatctgggcc tcaggattgg
      781 ctaagccgat atcttcatag gcgataacag tcaagcgacg agcgagacta ggcagatccc
      841 cagcctcaat caagcgggca gtatagtgga gactggcatc cacatctgag ccacggatag
      901 acttttgcag ggcagataga acatcatagt gaccgtctcc atccttgtcc atagtgatgt
      961 agcttctctg aagactattt tccatgatgt ctaaggtgat gtggcgaatg ccctcgtcat
     1021 tctcaggggt agagagaaca gccaagtcca gtgagttaaa ggcagagcga aggtctccgt
     1081 ttgtagaggt tgcgatgaaa tccagcgcat cctcatctag ttctattgga aaatcaaaac
     1141 cacgttcagg gttacttaga gctatctgca gggcctcttt gacgtcttgg ttagacagag
     1201 gttccaactc aaaaatttga acacggctac gaatggcagg agtgacggaa aagaaaggat
     1261 tttcagttgt tgctccaatc atgatgacca gtccactttc caagagaggc aagaggaagt
     1321 cttgcttggt cttatctagt cgatgaattt cgtctagcaa taggacgaga ccaccagaaa
     1381 atttagcttc ttccgagatt tcttgcagtc gctttttact atcaactgtc gcattgaaag
     1441 ttcgaaaggc atacttggtc gttccagcga tggcagaggc aatactggtt ttgccgattc
     1501 ccggagggcc atatagaatc atggaggaca ggcggttggc ttccaccatg cggcggatga
     1561 tttttccagg tccgaccaga tgctcctgac cgatgacctg gtcgatggtt ttagggcgca
     1621 tgcgaagcgc gagattgtct ggcatagcag tcctttctag cgtggatttg agtagaatga
     1681 ctcatctact atatcttact atgtccattc tagcctatta gttgtgatat ttcaatggtt
     1741 ttgtaataat cagggagtga gatagaatct tttttactat ttaatttaat gaggagaggt
     1801 atcagcatga gagatttgat tttccttaaa gctcaaaata ggatagttga gcctaaagaa
     1861 gtggaagtgc tgacttgaga aataatattg ctgaatgtca ttgtatgaac tataatgaaa
     1921 agagaggtga taagcaatgg aaatgatgga actaccaagt caggaaattt tgatttttac
     1981 aaaacaaatt cgtcattgga ttcttagtga tcaagttatt tcagggaaaa gaaaactttt
     2041 cttccgggaa gatactccaa aagagatttt agatttgtac gaaaacatta aatctaaact
     2101 tgattttgct taccaagaag ttcattctaa taatgggttg aaaaaatatg aaaaatagaa
     2161 taattgatgt ttttgaagta gtaaatcgcc tccttgtaat aactgttgaa aatcctgatt
     2221 tcgaagactt gagagttaat cagtttgtaa aaattggaga taaaaagtat agagtacgca
     2281 gtgttcctat gattcattca actccgcctc aatctgtctt agatcgagat acttttacaa
     2341 ttgactatac agatgatgaa ttgctcgata aagaagcagt ttttactact cattagaaaa
     2401 ataggtatga ataaattggc ttcccttaga accacatcta ggggctttta tgtataatag
     2461 aataataagt atcactcctt tacaatacgt gtaacacgtg ttataatggt gttgtcagga
     2521 ggtaaaagcg ctatgcctat gacacaaaaa gagatggtta aactccttac ggcccatggt
     2581 tggataaaga ctagaggcgg taaaggctcc catattaaaa tggaaaagca aggggaaaga
     2641 cctatcacaa tccttcatgg tgagctgaat aagtacactg aaagagggat aagaaagcaa
     2701 gctgggttgt aaaattcagc ccctgctttt tgatatggag gtagtgaaat gttagttacg
     2761 tatccagctt tattttatta tgacgacaca gacggaacag aagcgactta ttttgtccat
     2821 ttcccagatt ttgaatactc agctacacaa ggagagggga tttctgaggc tttggctatg
     2881 gggtcggagt ggctagggat aactgttgca gatttgatag agagtgatgg agagttacct
     2941 cagccatcag atatcaatag cttgtcttta attgataatg atccttttaa agatgatgaa
     3001 gatttcgtgt caacctatga ccttgataaa tcttttattt ctatggtatc tgttgatgta
     3061 tcagaatacc taggaagtca ggaacccatt aaaaagactt tgaccatacc aaaatgggca
     3121 gataagttgg gacgagaaat gggacttaac ttttctcaga ctttgacaga cgctattgca
     3181 gataagaaag ttcaagccta agattaagcg tggtatcgtg gtaatagtca ataaaaaagc
     3241 acgtccactt gtgctagtta ctcgcctatt gattttatag atttattagc cctttcgagg
     3301 gctttttata ttgattttta tgagaaatga agaaatcaga cttttaagaa aatcactgat
     3361 accaagggtt taaatgaggt aatatggtat aattaggaca taaaataatt ttgtggtaag
     3421 atggtagtat ctattttagc atatttccga gcaatcgggg cgattaaaga gtcgcataga
     3481 aagaggacaa aatggcaaca tacggatttt tagatatttt agaggaagag ttggacaaga
     3541 actttccctt tgactttgag attagttggg acaagcgtaa ccacgcggta gaagtgagtt
     3601 ttctattgga agcgcaaaat gctgcaggtg tggagatggt ggatgaagat ggagaggttt
     3661 catcagatga cattcttttt gaggaagcgg tccttttcta caatcctgcc aagtcaaccg
     3721 tcaatgcaga agactattta acggttatcc cttatctacc taaaaaagga ttttctcgcg
     3781 agtttttagc ttattttgcc cttttcctca aagatactgc ggaggttggg ctagataccc
     3841 tcatggactt tttggaagat ccagaagcag aggaatttgt tatggaatgg aatcaagaag
     3901 tctttgaaga aggaaaagtt ggcttggaag agggagaatt ttatccttat ccaagatact
     3961 aggagttggt tggaggtgtt atgaagaaaa ttgggactta tttggtttat gtgctagctg
     4021 ttgtctttat tatgctagct tttgcttgtg gaacaatcgc atttgcagag ttggggtatt
     4081 ccgcagtttt agcctttact tttggttatg ccttcgctct tctaagcatg tatttaatct
     4141 ttattcttca tgagctgggt catgcttttt gtggctactt gacaggctat cggctggtgg
     4201 cttttggatt aggacatttt attttgacca aaaagtcagg caggtttcat cttagtagaa
     4261 cagccattct gaaaaatgtt ggtgctcaat acattggttt aaaagaggat gaaagcgatc
     4321 aaagaatcat cctgatgctt tcaggaggct tgatggttca tctcggcttg atattattgg
     4381 cgatagtgtt tggatttttg acaagaagct ggtattttgc agggacttgg atttttctta
     4441 atttatcttt cttcctaaat aacattttgc cagtcgacat caccgatgga gcaaaaattt
     4501 gggaattact acaacaccct gaaaatacca aatacgccta cctgatgttg aggcattctg
     4561 cccagacctt gctagctcct caagaatatg atttaaaaga ctttgttcaa gcagttcctg
     4621 aggatgcacg agggagtttt gcggatggtg tattaggtat gcaaggtgaa gtttccattt
     4681 tggaagggaa ggaagaagta gctaaacagc agtttcaagc attacttgag aggacaacta
     4741 ctccgatgat gcagacagtt gcccaactat ccctgcttca tatcgccttg ttggaaggag
     4801 attttgagca agcggagcaa tatgctagta ttcgacaggt caaatcgttt ctgtctctca
     4861 aactgtccaa tcttcaagtg atacaagctt ggtatcaatt taaggtaaag aaagatgtag
     4921 atcgaactcg taaggctctc aagattgcta gacagaaaat ggatagtagt cgaatgttga
     4981 gggacgagaa acgctattgt gaaaacttgt tagcagagct ggaaaaggaa atgttagaag
     5041 gaactgaaga tggaactaga aatttctgat ttccaaggtt gcaagattgc cctcttttgt
     5101 ggggataagc tactgacgat tttgcgtgat gacaaggacg atatcccttg tcccaacatg
     5161 tgggagttgc caggtggtgg ccgtgaaggg gacgaaagcc cttttgaatg cgctcggcgt
     5221 gaagtttatg aggaactggg aattcattta gatgaagact gtctgctttg gagcaagatt
     5281 tatcctagcg taatttttaa aggcaagaaa tcggtcttta tggttggtca gctaagacaa
     5341 gaacaatttg acaatatcat cttcggagat gaaggacagg gctataaatt gatgcctatt
     5401 gaggaatttc tcaattctaa acaggcagtg cctcagttgc agggaagatt gagagattat
     5461 ttggaggaaa gtttatgata acacttgttc gagcgggagc agaggattta gaaactatta
     5521 ttgcaattca gagagccagc tttaaggctg tttatgaaaa ataccatgat gaatatgacc
     5581 cttatctcga ggactgcgaa cgaattaagt ggaaattagt cgagcgtccc aatagctatt
     5641 actactttgt aaaagaagat gaagaaaaca tcggattttt acgagttcag accaatgccg
     5701 agttaacgaa agcttggttg ggaacggcag cgattttgcc tcagtatcaa ggaaaagggt
     5761 atggatctaa aggtttaagc ttactggaaa aagaatttcc aaccatcaca caatgggatt
     5821 tgtgtacggt gttacaggat gcaggcatgg ttgcttttta cgagaaaaac ggctatcgtc
     5881 aaatccacat tgaggctgaa aaagaaggca tggatatggt ctacatgaaa aaattgatag
     5941 agaaatagaa aggatggttc ggttaaattt ctaaactgaa cccgccctaa acacggtgcc
     6001 aaaaagataa acttctctta gacacaaacg tcttcagaga gtttcctatt ttggctttgt
     6061 gttttacggg cttggtatct taataatgga aacatggcaa gagttaaaag ttacagtgaa
     6121 gcgtgaggga gaggagttgg tttccaatct cttgattgag ttaggtgccc aaggggtagc
     6181 aattgaagac agcatggact atgtgggaaa tgtggaccga ttcggtgaaa ttttcccaga
     6241 ggtggagcaa caggaagaaa tcgtagtgac cgcctactac cctgaaacgg ttgatgtagc
     6301 agcagttgaa gcggatttgc aggctcgtct ggcagaattg accgacttta tggacctagg
     6361 agaagtcaag atgggcacga ctgccttggc tgaggaagac tgggctgaca actggaagaa
     6421 gtattatgaa ccggcacgca tcacccatga cctgaccatc gtaccgtcat ggacggacta
     6481 tgaggcaaca gcaggagaaa agattatcaa gctggatcct ggtatggcct ttgggacagg
     6541 gacccaccca acaaccaaga tgagcctctt tgccttggaa caggttcttc gtggtggcga
     6601 aacagtgcta gatgtaggga ctggttcagg ggttctttct attgcaagct cactcctagg
     6661 tgccaaggag attttcgcct atgacttgga tgatgtggcg gttcgtgtcg ctcaggaaaa
     6721 tattgagctc aacactggta tggaaaatat ccatgtagca gcaggagact tgcttaaggg
     6781 agttgagatt gaagcagatg tcatcgtggc taatatcttg gcggatatcc tcattcatct
     6841 aatagacgat gcctatcgtt tggtcaagga cgaaggctac ctgatcatga gtggcattat
     6901 caaggataag tgggacatgg tgcgcgagtc ggctgaatca gctggatttt tcctcgaaac
     6961 tcacatggtt caaggggaat ggaatacctg tgtctttaag aaaaccaagg atatctctgg
     7021 tgtgattgga ggctagcatg cagcagtatt ttgtaaaagg cagtgctatc tctcctgtca
     7081 ctatcgagga caaggaaacc agtaagcata tgtttcaggt tatgcgcttg aaagaagagg
     7141 atgaggttac cttagtcttt gatgatggca tcaagcgctt ggcgcgcgtg ctggatatgg
     7201 aaaatcgtca gtttgagttg gtccaagaat taaatgacaa tgtggaacta ccagtccaag
     7261 tgaccatcgc atctggattt cccaagggag acaagctgga gttcatcgct caaaaagtaa
     7321 ccgaactggg ggctagccaa atctgggcct ttcctgcaga ctggtcagtt gccaagtggg
     7381 atggcaagaa attgggtaaa aaagttgaaa aactagaaaa aattgccctt ggagcagccg
     7441 agcaaagcaa gcgtaatatt gtaccaagta ttaaactttt cgagaaaaaa gcagattttc
     7501 tagctcagct ggaccagttt gactctatca tagtagccta tgaagaatca gctaaagaag
     7561 gagaagccgc tgcgctctta caagcggtct ctggtcttga aacaggagcc aaactgctct
     7621 ttatctttgg accagaaggc ggtctctcac ctgcagaaat cgaaagtttt gaagctaagg
     7681 gagccgtttt ggtaggccta ggtcctcgta ttttgcgagc agaaacagca ccgctttacg
     7741 ccttatcagc ccttagtgtt ttagtagaat tagagaaata agaggaagaa aatggaacaa
     7801 aaacaccgtt cagaatttcc agagaaggaa ctctgggact taacagccct ataccaagac
     7861 cgtgaggatt tcttgcgtgc aatcgagaaa gctcgcgaag acatcaacca gtttagccgt
     7921 gattacaagg gcaatcttca cacttttgag gatttcgaga aggcctttgc ggaattggaa
     7981 cagatctaca ttcagatgag ccatattggc aactatggtt ttatgcctca gacgacggac
     8041 tatagcaatg acgaatttgc caatattgcc caagctggga tggaatttga aacagatgcc
     8101 agcgtagcct tgaccttctt tgacgatgcc ttggtggtag cagacgagga agtcttggac
     8161 cgtttgggtg aattgcctca cttgacggca gctattcgtc aggccaaaat caaaaaagcc
     8221 cactatctag gggctgatgt ggagaaggcc ttgaccaatc tcggtgaagt tttctatagt
     8281 ccgcaggaca tttatactaa gatgcgagct ggggattttg aaatggctga ctttgaagcc
     8341 catggcaaga cctacaaaaa cagctttgtc acctatgaga atttctacca aaatcacgag
     8401 gatgccgagg ttcgtgagaa atccttccgt tccttctcag agggacttcg taagcaccaa
     8461 aatacggctg cagcagccta tctggctcag gtcaagtctg aaaaactctt ggctgatatg
     8521 aagggatacg actctgtctt tgattatctt ctagctgaac aagaagtgga ccgtgtcatg
     8581 tttgatcgcc agattgacct catcatgaag gactttgcac cagtcgctca gagatacctc
     8641 aagcatgttg ccaaggtaaa tggtcttgaa aagatgacct ttgcagactg gaaattggac
     8701 ttggacagcg ccctgaatcc tgaagtgact attgacgatg cctatgattt ggtcatgaag
     8761 tcggtagaac ctttggggca agaatattgt caggaagttg ctcgttacca agaagagcgc
     8821 tgggtggact ttgctgctaa cagtggcaag gattccggtg gttatgcggc ggacccatat
     8881 cgcgtacacc cttatgtact catgagctgg acaggccgtt tgagcgatgt ctataccttg
     8941 attcatgaaa tcgggcattc tggtcaattc atcttttcag acaatcatca aagttacttc
     9001 aatgcccata tgtcgaccta ctatgttgaa gcaccgtcaa ccttcaatga attgctactc
     9061 agtgattact tggagaacca gtctaatgac ccacgtcaaa aacgcttcgc tctggctcat
     9121 cgcttgacag acacctactt ccataacttt atcacccacc tcttggaagc agccttccag
     9181 cgtaaggtgt atacattgat tgaagaaggg gagacctttg gagcaagcaa actcaacagc
     9241 attatgaagg aagttttgac agatttctgg ggagatgcta ttgaaattga tgatgatgca
     9301 gctttgactt ggatgcgcca agctcactac tatatgggct tgtatagtta cacctactca
     9361 gcaggactag tcatctcgac agctggttac cttcatctta aaaactcaga aactggagct
     9421 gaagactggc ttaatctcct caaatcaggt ggtagcaaga caccacttga gtcagccatg
     9481 attatcggtg ctgatatttc aacagacaaa ccactccgtg ataccatcca attcttgtct
     9541 gacacagttg accagattat cgcctacagt gctgagttgg gggagtaagg ggatagagag
     9601 gttgttatag aatgggtatg gacagttatt tgcttaaaag tccatgttta aatcccccat
     9661 tccttttcga ggaattcttt gcccttctct gtaatggtaa gtgaacgtgg cgtttgtgca
     9721 gggatgacca attgtttttc actgaaaaat tctaaaagag cattgccgag agctccgccg
     9781 acatgatttt tacgttcgct ccaatccaaa catttagggc aaaatttccg ttttttcttt
     9841 ttcaattcat ccgtatcaat gtgtaatttt ctttggaaat aagacttccc agcttcagtg
     9901 agttggaagt ctttttctga ttcgctgagg agattttctt gcaagagata attggtaatg
     9961 gaaatgccaa gctcaccagc aatatggtca tagcaggtgc gagcgacctt taattccttt
    10021 ttgtgaatgt gttgtttata acttttgaca tcacgctgag gagagtagaa gctgagaaat
    10081 tccagcattt ctgcaatggc agcacttttt aaagaataat aatgaaaacg tccataggtt
    10141 tgtagagtgg ttacctgcgc ttcggtaagt aatttgaggt gatagctgac agtatgttgc
    10201 ttgattttgg taaagctggc aatttcgtta accgtgtgag cttttccatc cattagaata
    10261 tctaaaatgt gcatccgaga agggtctgat aagagttttg tgatgatgaa aatatctggt
    10321 tccataaaaa ttttctccaa atttc
//
LOCUS       DAVMGZ010000066        10308 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000066 DAVMGZ010000000
VERSION     DAVMGZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.067"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..274
                     /locus_tag="VYQ93_001321"
                     /pseudo
     CDS             1..274
                     /locus_tag="VYQ93_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(402..1424)
                     /gene="cbpF"
                     /locus_tag="VYQ93_001322"
                     /pseudo
     CDS             complement(402..1424)
                     /gene="cbpF"
                     /locus_tag="VYQ93_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
     gene            complement(1443..2264)
                     /gene="cbpG"
                     /locus_tag="VYQ93_001323"
     CDS             complement(1443..2264)
                     /gene="cbpG"
                     /locus_tag="VYQ93_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEV3336003.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYLSYNFKIHQMYTDKKQVLSDDGMFLDYQVNTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYYYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGNWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2345..2584)
                     /locus_tag="VYQ93_001324"
     CDS             complement(2345..2584)
                     /locus_tag="VYQ93_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336004.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(2734..3000)
                     /locus_tag="VYQ93_001325"
                     /pseudo
     CDS             complement(2734..3000)
                     /locus_tag="VYQ93_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3025..3336)
                     /locus_tag="VYQ93_001326"
     CDS             complement(3025..3336)
                     /locus_tag="VYQ93_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV3336005.1"
                     /translation="MDWDFADTCWEKDPREYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHSELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(3261..3502)
                     /locus_tag="VYQ93_001327"
                     /pseudo
     CDS             complement(3261..3502)
                     /locus_tag="VYQ93_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3503..4135)
                     /locus_tag="VYQ93_001328"
     CDS             complement(3503..4135)
                     /locus_tag="VYQ93_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3336006.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(4149..5144)
                     /locus_tag="VYQ93_001329"
     CDS             complement(4149..5144)
                     /locus_tag="VYQ93_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3336007.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FFFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5141..5839)
                     /locus_tag="VYQ93_001330"
     CDS             complement(5141..5839)
                     /locus_tag="VYQ93_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV3336008.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(5916..6926)
                     /locus_tag="VYQ93_001331"
     CDS             complement(5916..6926)
                     /locus_tag="VYQ93_001331"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV3336009.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(6910..7917)
                     /locus_tag="VYQ93_001332"
     CDS             complement(6910..7917)
                     /locus_tag="VYQ93_001332"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV3336010.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRADIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFFAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTETLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(7904..8857)
                     /gene="mvaD"
                     /locus_tag="VYQ93_001333"
     CDS             complement(7904..8857)
                     /gene="mvaD"
                     /locus_tag="VYQ93_001333"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV3336011.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(8839..9717)
                     /gene="mvk"
                     /locus_tag="VYQ93_001334"
     CDS             complement(8839..9717)
                     /gene="mvk"
                     /locus_tag="VYQ93_001334"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV3336012.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQAVLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(9839..10168)
                     /locus_tag="VYQ93_001335"
     CDS             complement(9839..10168)
                     /locus_tag="VYQ93_001335"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3336013.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNDTLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         3016 a         2414 c         1817 g         3061 t
ORIGIN      
        1 agcgaaatta tcgcctataa tctttcaact tcacccaact tagaacaagt acaaacaatg
       61 ttggaacagg cattcacaga gaagcactat ggtggagtcc ttctttggga ttctgaaatc
      121 ggaaatgttt tatggctatg agaaaacatt taaatcactt aatcaattgg aacaagccat
      181 tatagactat attgattatt acaacaacaa gagaattaag gtaaaactaa aaggacttag
      241 tcctgtgcag tacagaacta aatcctttgg ataaattatt tgtctaactt ttggggggca
      301 gtacagaaaa accttgatac tatgcgtttt ttgattgtga agacttttat ccacctatta
      361 tacaaagtta agtatccatc acagtttaca attagccttc attacttaac ccattcacca
      421 ttatagttta agtagtaacc acctactgtg gtattaacag ctaaagcacc tgaatcatag
      481 gcatagtacc agttaccatt gacttggaac cagcctgttt tcatatctcc attacttgca
      541 tttagatagt atcaagtcga accttcctga taccaaccag ttgccatagc tcctgatgaa
      601 cggagatagt accatttgtt cccaaggttt tgccagccag ttctcatatc accattttca
      661 gcatctagat agtaccaagt tgagccttcc ttataccagc cagttgccat agctcctgac
      721 gaatggaggt agtaccactt attacctaga tattgccaac ctgtttgcat aatgccagtt
      781 gctggatcta gatagtacca tggagctgct tttagcttct cttcatcata cgtaagaggg
      841 taatccttaa cccaaccacg tgctagctct ccaaccgtca atctgttgat gcgagaatca
      901 aagccaccat ccttctgtaa ataataccaa tgaccctctt cataaatcca accagttttt
      961 aaagtatgat aactacgctg atcttcaaaa taatagactc gtttctctgc ttggctatca
     1021 tattcttccc catgatgttt gttggtattc gtagcagttt ttgcttctaa aacttgcttg
     1081 ccaacaaatt cttgtaagac accatcttga ccaaaataaa accaatctgg tctaagagca
     1141 atctctattc ttggagaagg accaatcgta acccccttgt gtggagcagg tatatattgc
     1201 cagccgacaa ccatctctcc ggataaagga tcaaaataat agtacttccc atctatcact
     1261 ctccagtagg tttcttttag agcccccttt ttgtagtagg ttctaccatt ttctttgaca
     1321 aactgccatc cttcagaatc atctgcaaat actgtactgg tccctagcaa accgaagaaa
     1381 aatgtggcta gtgcgacttg catcattttt ttcaaaagct tcattttata tatcctccaa
     1441 catcaaatcc actcaccaga tgaagcgaag ttataaactt taccatcgat agtttggcta
     1501 cctgtaacca ttgctccatt tgaattgaga taataccagt ttccatttac tttttgccaa
     1561 tctgtaagca tcttaccgga actgtctaaa taataccaag tatcatttat ctcctgccaa
     1621 cccgtttgtt tatcatgttg tctataatag taccaactac catttatttt cttccatcct
     1681 tcaagagagt aacctttaag aaccgaataa ataaatggca aatttcgttc atttaattta
     1741 actgcactgt taatttgatt agctccatct cctaaagtat gcactcctac tacacggtga
     1801 ctagcatcat aaactgtaga tccactagac ccctctaaag tattaacttg gtaatccaag
     1861 aacatgccat catcacttaa aacttgtttt ttatctgtat acatttgatg aattttaaaa
     1921 ttatatgata gatagcctgt gatagtcaca gttattcctg tcaaattttt ttgactagta
     1981 ggaagaccca aagtccctaa ttttgcacca atgggctctt ctagaattaa taaagccaag
     2041 tcatattccc ttgcatcctt agaatttaaa tttctaaatt ccttcaaata acgaacttcc
     2101 tttactttga tctttccaaa tagttcttga cttggactaa cagccggaag aacataaata
     2161 tcatccgctt ctttcccata gtcatgtctg tagtagttgt gagccgctgt aattaacaca
     2221 ttatctttaa taaaattcgc acttcccctc ccttctacat tcattccatc ggctactcca
     2281 taatacttag ataaaaccat agctgaagtc gaatacggat actgtaaagt attatcaatt
     2341 ttaatcaaat catcattacc gataatactt ctgattgctt ttggtagtat gaaccatacg
     2401 ttggtgaaat ctcagataat gaagaatcat tagactctgg acctttttct agtgtctcac
     2461 ttacctcata ttcttcacct gtactagaaa taacactcaa agcagatact gtcgataact
     2521 ggctagccaa taaagtactc gcaataattg aaatacccaa ttttttataa acagttttct
     2581 tcattattgt atcctcctaa tgtaattata gcgtactatt ctaaatttct taatctacta
     2641 tagaatcaag aaatctacca ccttctttaa ataccctcca ttatcacata aacaggtaaa
     2701 cttttcaatt aatgactgcg cttttcaatc acgctagagg tacttgcttg cttctttgat
     2761 actaagttca gccattcttt ccttgttttt ctcaataaag catgttaccc aagtgggatt
     2821 cgttttggag tagtctcgca gagtccagcc aatggcttta ttgataaaaa attctgtttg
     2881 gttcaagtta tgaagtagaa tcttttccat taattgagta ttggtcttct cttttcttaa
     2941 caactggtgg tcaatagcga cacgtctcag ccagatatta tctgataggc tccattttat
     3001 actcaatgaa aatcaaagag caaactagga agctagccgc agattgctca aaacactgtt
     3061 ttgaggttgc agatagagct gacgtggttt gaagagattt tcgaagagta ttaagattat
     3121 ttcttctagt tcagagtgtt catacaccaa actccctact actcgatcta ggatatctac
     3181 cgtgtcccac caggattttg tcacgactaa ctgctctagc ttaggcaaat cggtttcctt
     3241 tagataagac tgcattgctt tcaaatagtt agcagccaca tattggtatt ctctaggatc
     3301 cttttcccag caagtgtctg caaaatccca atcgataatc tttgtttttt tcgcttctgg
     3361 aaaatatttt tatagagttt atttctttca ggcaccgcaa tacctagaaa agaaaattga
     3421 tggcgcatat aggcttccat gggccttgct ttttagagtc ttttgctgct tctagctcct
     3481 caagtaaatc tgctaaactc atctaaaact cctcatgccc caccaaatgg tgctgaaagg
     3541 catagacagc cgcctgggta cgatcgctga cttcaagttt ggcaagaatg ttggacacgt
     3601 gggtcttgac cgtcttgaga gagataaaga ggtcatctgc gatgcgctga ttttcgtagc
     3661 ccttggcgat gagttggaga acatctcgct cacgcgcagt caattcttca tgaagttcca
     3721 tatgattgcg gtggtattca accttcttgc taacctcttg ctcaatggcc agctcgccag
     3781 cagctacctt actgacggca tgaagcaatt catctgcact agaagtcttg agcatatagc
     3841 ctttggcacc agcatctaag actggcatga ttttttcatt gtccaaataa gaggtcacaa
     3901 tcaaaatctt ggcttcaggc cattctttaa ggattgctaa ggtcgcgtca atcccattca
     3961 tctcaggcat gacaatatcc atgacaatga catctggacg cagttccaag gccaagtcaa
     4021 tcccttgaga cccgttggac gcctcaccta caacttctac atcgtcttgg aggtcaaagt
     4081 agcttttcaa gcccaatcgg accatttcat ggtcatctac tagtaaaatt ttcatcttta
     4141 ctcctttatc attccttatc taacagggga atacggatat caaccgccag cccttgcttg
     4201 ggagctgtca agagttgaac tgttccagcc atatcttcaa cccgctcctt gatatttcgc
     4261 agtccataac tcaagtcgtc taagctccct aactggaaac caatcccatt gtccaccacc
     4321 ttcagttgca attcaacatc tgtctgatag aggtagacat ctaggcaaga tgcctgggca
     4381 tggcggagcg tattgctaat caactcttgc aggatacgga agatatgctc ctcgattttc
     4441 ttaggcaatt tcgtcatatt ctgcttgaga ctaaccctaa gatcactctt gtcctcaagc
     4501 tcttttaaaa gaatttgaat cccttctatc aagctcttct gctccagttc aactggtcgc
     4561 aaatgcaaga gcaaaacccg caaatccttc tgggctgttt ctaaaatagc tgtgacactc
     4621 tgcaactggg tctgcatctt ttctctatcc aatttcaaag cctgctgact gatacccgat
     4681 aaaatcatgt gggccgcaaa caactcctga ctgactgtat cgtgcaaatc ccgagcaatt
     4741 cgcttccgtt ccttctcgat gatttcctct tcctgagcaa ggctatgatt ttcagctttt
     4801 tgaagagcct ctgtcaaaag gttaagttta cctgataagg acttgaaact ggcatccaaa
     4861 tctggatcta caacctgaac cacttcttgc cctgccaata aacgcttgag attagcctgc
     4921 atttttctta gagaaagctc ttcgatccct cgccaaaaca gggctaagag acaggtcatg
     4981 gacatgctga aaaccaacaa taaaaagaaa aatttttctg ttttttcgac atcgtgcaaa
     5041 aagatagacc agtcaaaatc aagtatttcc agcaagctat gggagaaaaa aaagacaaac
     5101 aggaaggagg tgagagcgat aatgacatag gcttgttttt tcatcctcta accacctcaa
     5161 catcaccaat catagtggtc aagaaaatct tgacactctt gttactcttg agatagtctt
     5221 ttgtttcttg atgatagtgt tcattgcgga gggctcgctt gggctggttg aaaaaagtca
     5281 aatccccata gagacagtta acgctgagac tgacttccac atctacaggt acgatgattt
     5341 tggtcgttcc taccatcttt ctgaggataa tgacattgtc atgattggtt aagatgaccc
     5401 tctccagatg aatagtgtcc ttgcccatga agcgaaagag attgatatca tcaaattggc
     5461 aagtctggta gcttgaaaaa tgatgaagat ttccaaacca acgatttttc tccttcttaa
     5521 ccgtcacaac ctcttcaaaa accaaattgg tctgctcttt ttcctggttc atcatcgggt
     5581 aaagaagaaa gagactatag ataaccgcaa caaaaatagc tagaatcaca aaaggattga
     5641 gcataacgat gaaaaagaag agaatggttg ccgctactaa aagaagatta ttcccctctt
     5701 taccagtgta gtagcgaatc aaaagcaaaa agaggaatag tatcagcaga aaacgcgaaa
     5761 aatgctctga taccatcaaa atcagagctc ctgtcagaag acaggcttcg ataaataaaa
     5821 agattttaaa ttttctcata ggttcatcct ctcccttcta ttttatcaca attcaaaaaa
     5881 gtcacctcag tctgaggatg gaaaaaaggc gctggttacg cctttttcat ctgatccttt
     5941 gcttctttta attttccata aagaagatag tctacttttt gtagatctgc tatggtggca
     6001 cagttaaggg aacacataat caagcgtagg tctgctttcc agccttggac aatgccaatc
     6061 acttcttcaa ctgtgtaggt ttcaaccaat tccagaacgg ttcgtgacaa tcccacagac
     6121 ttagcaccaa aaaccaagca cttaatcata tccagcggat tccgaacccc tccactaacc
     6181 aagagttcga ccttatcttt ccattcttgg gcattgagaa gggcctgcat ggtagactga
     6241 ccccattgat tgaggtaatc acgctggcca ctacgacggt tttcgatata ggcaaagctg
     6301 gtgccaccac gacccgatag gtccactgta cgaacaccga attcataggc tctttcgatt
     6361 gtcttggcat ccattccaaa gcccacttcc ttgaggacaa taggaacggg aatttgctcg
     6421 ctataatctg ctagatgcga ttgccagctt ctaaacttcc tttctccctc gggcatgagt
     6481 aattcctgca tgacattgac atgcacttgc aatagaacag gattcatctc ttctacagtc
     6541 tgaagtccta actcgacagg cttgtccaat ccaatattgg ttccaaggag gagattggga
     6601 tgactagact tgacagaaaa agaatcatcc gttggatttt tgagggctgc gctataagaa
     6661 cccgttacaa ataaaatacc acaggattcc gccacctgag ccagcttttg attgatttct
     6721 cttcccttat tacttccacc agtcatggca ttgatataaa aaggaaagtc ccactttcga
     6781 ccagcaaact ctgtcgaaag atcgatttca tccagattgt aaagaggcaa ggaagaatga
     6841 atcagctcca cctcatcaaa gctattatag gaacttttct gctcaagggc atagaggata
     6901 tgctcgtcct tacgatttgt cgtcatgtcc tatcctttct tgatataaga gctcaatccc
     6961 cagatcggcc caacgatttt ttaaggtttc ggttgattgc gcatcaaaac tcaaggcgat
     7021 gccacagtca ccaccaccag caccactact cttggcaacg gcctgcaaat cttgactggc
     7081 ttctttcaac tgtctaagca aaggcgtgta aatatctgta ctcaagcctt ctaaaagctt
     7141 gctggctact tctacttgct cgataacttt ttcggctttc ccctgctcca aggcttcgac
     7201 caaagaaacc accgtttctt ttgaggaact taaaaaattt tgattgatat tttgcttgat
     7261 ttgctggacc atgtgactcg atacagccac ttccttggtc catcccacta agaaatcaca
     7321 ttctaaagtt ggtttcactt gtgagataaa aaatccccaa tcacgctcca gaactgtcgc
     7381 caagttttct tcttctaacc aagcagccac cttctggcga tcaaatgact ggtagagaac
     7441 caaatcctct gccacaatac aggcaaggtc gcccatggaa ccattgtctc ctcgcttgag
     7501 caagacagcg ctagtcagct tgaacaagag ctcctgatca acagaaacat catacagagc
     7561 cagtaaagcc ttgacaacca agacaacgac gctgccacta gaacctagac caaacttttt
     7621 cccttctcgt tccattttgc cacagatttc tagggaaaaa ggtcttaaat tctgaccgcg
     7681 aacagcgaag aagtctccca tcaaagcaat cgtttcttga atcaagctgt agtcaggatt
     7741 gggccttaag tccactgcga aatcaaacat atctgaatag atacggtagc tgtcagaaaa
     7801 agcaatatca gccctcatat agatgggaat atcctttatc aaagctaact gccctggctc
     7861 taaaatagca tattcacctg cccaatagag ttttccgcaa gttttaacag caatcatctt
     7921 gactcaaatc ctttgttttt gacacaatca agcgataacg ctgaccgaaa atttctgata
     7981 aatgctccaa gtctttctcc tgacagaaga ccttaacatt gggaccagca tccatggtaa
     8041 agtagcaggc ctctcctttc tcacgaagct ggcgaacaaa gtccatagcc tcataagagg
     8101 catccgtcag ataagaaaag gctggactag cagtctttgt cgtagcatgc atagccaggg
     8161 catttttctc cgttaattct ccaatcttgg caaaatcatt ttccttgaga taaatcagca
     8221 tatcctgata gtccttctca gactgacgaa cccagtcgtc aaaagtcgtc gaggtttcca
     8281 cacaaagttt catcccatca cggctagaga ttggtttttt cttgtcctct agcaccaaca
     8341 taatcatagc tagtttcaag tctgtctcta cagggtaaat ttctccacta tccttatccc
     8401 aggctcctag tggtccataa aaactccgag aagaagagcc tgaggcaaac ttggcttcct
     8461 gcgctaactg acttctatcc aatccaagct tgaaataagc attacaagcc ttgaccaggg
     8521 cggacaaacc actagaactt gaggacaggc ccgctgccgt aggcatattg ttttgagtat
     8581 cgatacggac aaagccctca ccagctggac gataacggtc aataatctta ctcatcttgg
     8641 catgctcgac ctcattttgt agctgaccat tgatgtaaaa ttcgtcagct gttacattgg
     8701 ctggtaaagg cgacaaggtc gtctctgtat acatattttc caaagttaga gaaatactgc
     8761 tagtagcagg caccatctct ttttcttttt tctttcccca atatttgata atagcaatat
     8821 ttgcgtagga acgtactgtt acaggctctc tatccatgtc tgaacagctc ctttctcttc
     8881 taatctttct gctagttctt gtgcgtgtgt caaattggtt accaaggcta tgatacaacc
     8941 tcctagccca ccaccgctca tcttggcacc cagagcacca tggctaagag tcgtttcaac
     9001 caaaaagtct gcctcagggc tactgactcc aatttctttt aaatgtaaat gcgcttgact
     9061 gaggatttgt cccagtcctt cagcatcttt ttgtgaaatc gcaacttctg cttgctgggt
     9121 taattctccc aaggcatgca aaaacggtag ggcatccttg cccttatttt gaaccacttg
     9181 gatggcttca cgagtatgac cataaacacc cgtatcggca atcaccaaat aggcggataa
     9241 atccatctca agttctgtaa atcctacgtt cttgataaag cgaataggtt ggtcactgag
     9301 acaggtctta gcatccaaac cactaggatt catatgggca atcatttcag ctcgattgac
     9361 caagatttct agtacatcat gaggcagaac agcctgatag tagtcaaata ccgcacgaat
     9421 ggccgctatg ctgatagccg ctgacgaacc catcccccgt ttctcaggga tagccgagtc
     9481 aatctcacaa cgaatgcagg cttctgtgat atccaaatac tccagtgagg cataaaccgc
     9541 catggacaag gtatcctcct cataaaggcg ccaaggactc tctgcaggaa ctaccttaca
     9601 ggtcacctcc acctccaaaa gaggcaggga aatggcagga taaccgtaaa cgaccgcatg
     9661 ttcccctatt aaaattatct tactatgtgc ctggccgaca ccaacttttt ttgtcatttt
     9721 ttccttttac tagacgaaaa aacgtcttat ttttcataca aatattaatt ctttcctatc
     9781 tattttatta tattttcaca aaaaaagcga ttgtttccat tcacaatcgc ttctttcatt
     9841 attgaaccca ttcgccatta tagttgacag aatagccatc tacggtcgta ttcactgcca
     9901 aggcacctga gctataagcg tagtaccatc tgccattgac ctggaaccaa cctgtcgtca
     9961 tagaacgacg aaagaaactc cataccatta agtaaagagg aaagtcgtga gggagcatgc
    10021 gccattgaca acctgtttta gtgacgtaca aagtatcatt aacaagtact cgtttcggcc
    10081 atttataggt gcggtgtttg gagaaatagg gttcaatctt cgcccattct tgatcgttta
    10141 aatcagtatc atatgcttta cgtatcataa ctctagttta acattttttt gtgaatacag
    10201 gttctaaata atcgaccacg aaaatttctt aagtggaaaa cgccttatga agtatgctac
    10261 gggaaagtta tgcacttaat ttgacaattc aagatgtaaa aatatata
//
LOCUS       DAVMGZ010000067        10017 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000067 DAVMGZ010000000
VERSION     DAVMGZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10017
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.068"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(36..1739)
                     /gene="ilvD"
                     /locus_tag="VYQ93_001336"
     CDS             complement(36..1739)
                     /gene="ilvD"
                     /locus_tag="VYQ93_001336"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV3336014.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(1972..2904)
                     /locus_tag="VYQ93_001337"
     CDS             complement(1972..2904)
                     /locus_tag="VYQ93_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV3336015.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(2901..3758)
                     /locus_tag="VYQ93_001338"
     CDS             complement(2901..3758)
                     /locus_tag="VYQ93_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV3336016.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRLTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(3762..5108)
                     /locus_tag="VYQ93_001339"
     CDS             complement(3762..5108)
                     /locus_tag="VYQ93_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV3336017.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(5121..5405)
                     /locus_tag="VYQ93_001340"
     CDS             complement(5121..5405)
                     /locus_tag="VYQ93_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3336018.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(5409..7439)
                     /locus_tag="VYQ93_001341"
     CDS             complement(5409..7439)
                     /locus_tag="VYQ93_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV3336019.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            7631..8638
                     /locus_tag="VYQ93_001342"
     CDS             7631..8638
                     /locus_tag="VYQ93_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV3336020.1"
                     /translation="MTEKLINSKPNGVFALILIELTLVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQNRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            8641..8841
                     /locus_tag="VYQ93_001343"
     CDS             8641..8841
                     /locus_tag="VYQ93_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV3336021.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            8993..9175
                     /gene="rpmF"
                     /locus_tag="VYQ93_001344"
     CDS             8993..9175
                     /gene="rpmF"
                     /locus_tag="VYQ93_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV3336022.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            9191..9340
                     /gene="rpmG"
                     /locus_tag="VYQ93_001345"
     CDS             9191..9340
                     /gene="rpmG"
                     /locus_tag="VYQ93_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV3336023.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(9820..>10017)
                     /locus_tag="VYQ93_001346"
     CDS             complement(9820..>10017)
                     /locus_tag="VYQ93_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336024.1"
                     /translation="ESGSMATGWVKDKGLWYYLNESGSMATGWFTVSGKWYYTYNSGD
                     LLVNTTTPDGYRVNANGEWVG"
BASE COUNT         3255 a         2126 c         1610 g         3026 t
ORIGIN      
        1 gttgacgtga tttgaagaga ttttcaagta tgagtttatt ttttacctga cttgtccata
       61 ttccagaagt ctgtcacggc tccgcgtgaa gcagatgata cgatatgggc atatttaccg
      121 aggacaccac ggctgtaaag tggtggcaag gttgtttctg ccttgcgttt ttcaagttct
      181 tcttcggata cggccatgga aatttctttg gtatcttggt caaccgtaac gatatcgccg
      241 gtacggagat aggcaattgg tccaccatcc tgagcttcag gagcgatatg tccaacaacc
      301 agaccataag taccaccaga gaaacgtccg tccgtcaaga gggccacctt atctccctga
      361 cctttaccaa caatcattga agaaagtgac agcatctcag gcataccagg accaccttta
      421 ggtccaacaa aacgaacaac gactacatcg ccatcaacga tttcatctgt cagaacggcc
      481 tgaatcgcat cttcttctga gtcaaagacc ttagctggcc caacgtgacg acgcacttta
      541 acacctgata ccttggcaac tgcaccgtca ggagcaaggt tcccgttcaa gatgataagc
      601 ggaccatccg cacgttttgg attttcaagt ggcatgataa ctttttggcc tggagtcaag
      661 tctgcaaagt cagccaagtt ttcagctaca gtcttaccag tacatgtgat gcgatctccg
      721 tgaaggaaac catttgccaa caagtacttc ataaccgcag ggacaccacc gacttcgtag
      781 aggtcttgga agacatactg accagatggt ttcaagtcgg ccaagtgagg cacacgttct
      841 tgaatcgtat tgaagtcctc aagtgacaag tcaacatttg cggcatgggc aatggcgagc
      901 aagtgaagag tggcgtttgt agaaccaccg agagccatcg ttacagtaat agcatcttca
      961 aaggcttcac gagtcaagat atctgatggt ttgagaccaa gttccaacat cttaacaaca
     1021 gcacgtcctg ctgcttcgat atcttctttc ttatcagctg attcagctgg gtgagaggat
     1081 gaccctggca aactcatccc tagaacttcg atagcagttg ccatggtatt agcagtatac
     1141 ataccaccac aaccaccagg gccagggcag gcattacatt caagacgttt cacgtcctca
     1201 gctgtcatgt caccgtggtt ccattttccg ataccttcaa agacagaaac caagtcgata
     1261 tctttaccat caagatttcc cggtgcaata gttccaccat aggcgaaaat agctgggata
     1321 tccatattag caatagcaat catagatcca ggcatgttct tgtcacagcc accgatagcg
     1381 acgaaggcat ccacgttgtg accactcata gccgcctcga tggagtccgc gatgatgtca
     1441 cgagatgtta gagagaaacg cataccaggc gttcccatag cgatcccgtc cgctacggta
     1501 atggttccaa actgtacagg ccaagcgcct gcagatttga caccttcttt agccagtttc
     1561 ccgaaatcat gcaagtgaat gttacatggt gtattttccg cccaagtcga aatcactccc
     1621 acaatcgatg tttcaaagtc cttatctgtc ataccagtcg cacgaagcat agcacggtta
     1681 ggtgatttaa ccatgctgtc atagatgcta ctgcggtgac gtttatctaa ttcagtcatc
     1741 ttatccctcc catttcagtt tttactatta tagcacaatt taaaggcaat gaacagaata
     1801 aaattcttga attttcagaa aattctatac gcatgtgaaa tatttaaaat taaaaacaac
     1861 aaagcggatt agtgcacttt ctgatgacca gaatatgctt tttaatccgc tttctttaaa
     1921 taacgtactg taatttttac agaaattctt tcagataagt gtatttaaca tctatcttgc
     1981 attataaatt tctagaacct tctcttttat attcgattca ctcaaaccat actcattaag
     2041 aagataatcc attttcccta cttgaccaaa tctttcttga acacccatcc gatgaatttt
     2101 tgtcattcca tcatcagaga ataattcaca taaagaactg ccaattccac ctatctgatt
     2161 gtggttttct acagtaaata tagtttttcc acttaacatt gtttttatct gttctggtat
     2221 cggtttgatt ctaaataaat ctatcacact tactgaataa cctaatttag acagttcatc
     2281 tgcaactcga atacttggag caaccattat accagaagca acgattacaa gatcttcacc
     2341 atgccttaac tcaatgtagc ctttagaaaa atcttctcca ccttgataca caggaactgg
     2401 agcttttcta attgttcgaa tatattttag tccttttaag tccaatgtct ggttcaatat
     2461 ttcacgaaat tggatatcat cagttgcttc aaaaatgatc gatttaggaa ttaaacgtaa
     2521 caatccaatt tcttcaaatg gcatatgtgt cccaccattc atttctgcag ttactcctgc
     2581 atctgatcca atcacagtgg catccaattg tgcgtatcca agagaaataa ataattgatc
     2641 aaatactctt cgtgaagcaa aaggaccaaa tgtatgaaga taaggtctaa acccctgaat
     2701 agacaagcct gctgcaaggc cgaccatttc tgcttccata atcccaacat tcacataacg
     2761 gtctccaaag tccttttcaa gattatgagt agccatcgaa cttgacaaat cggcttctaa
     2821 gactactata tcagaataac tttgattagc ctctaaaagg aagtctctat atacatgccg
     2881 taattctttc gtacttctca tcattctgtt tcctccaatt cctgacttaa tctttctaca
     2941 actgaagtta acatttgttt ctcctctaca gtaaggcgaa gatgatgatt ggatttcatt
     3001 tcttccagct cttgaacccc ttgaccttta atagtatcta atacaatgca cttaggtgat
     3061 gaattatttg actgttttaa ttggacaatc ccttcataaa tttctctaat atctgaaccc
     3121 ttgaccctaa tggattcaaa tccaaatgct gaaaattttt ctacgaaatc acctggatta
     3181 caaatatcct ttgtaaaacc atctaattgt tttttgttat catcaacaaa tacaattaag
     3241 ttggataact gttgatgaga agcaaactgt atagcctccc aacattgtcc ctcatttaac
     3301 tcaccatctc caacaatagc ataagtataa aagggactct ttcttattct ctgaccatat
     3361 gcaagtccag ttgcaacact aattccttgt cctaaagagc ccgttgtcat atctatgcct
     3421 ggcgttagat ttctatcagg atgagacggt aatttggttc catttgtatt taaagaatat
     3481 aggaattctt tgtcaaagaa accattcaaa tagagtgtac tgtatagagc tggtcctcca
     3541 tgaccttttg ataatatgaa ataatctcta tctcgtgctg caaatatttc tggagtcatt
     3601 ggcattattt caccataaag cactgctaaa acttctacga tagacagact tcctccgtaa
     3661 tgtccgaatc caagatgatt caatgttcta agagtattta atcggatgtt agtcgcaaat
     3721 tttcttaacc catcttctct atttttactt aaaatcatcc cttattcctc ctttgcagat
     3781 ggctttttaa taaaggatac tccaaacata actgctagaa taagaacaag accaatcaca
     3841 atgcctgctt gtgagccaaa ttgatttaac attcctaaaa taattcctga tagaccaaaa
     3901 tctgcatctg agaaagttga tccttggaaa ccaagtcctc ccaaaactgg cattaaaaag
     3961 actggaagaa aactgattaa aataccttgt aaaaatgctc caatagtggc tccacgaaca
     4021 ccaccagatg cattcccaat gacacctgca gtcgctccac agaagaaatg aggcacaaca
     4081 cctggtaaga taacaaccgt tcctgaagca atcataattg ccatacttac taaaccacca
     4141 acaaaactag agataaatcc aattagaact gcattgggtg cataagtata aacaatcgga
     4201 caatccaaag caggttttga attaggtaca agacgctctg aaataccttt aaaggctgga
     4261 acaatttcac ccaaaataag gcgaacacct gctaaaataa caaatacccc tgctgcaaat
     4321 tgacctgcta attgtaaagc ataaactaga ccacttgtac cactactgat ttctttttct
     4381 atatattctg accctgcaaa gatagctaca ataatgtaaa taactgccat ggataaagtg
     4441 atactaacag tactatcacg taaaaaagct aaactctttg gaaatttaat gtcctctgtt
     4501 gattttgatt tgtcaccgat aaggctacca gtaaaaccac tcaaccaata tcccaaagaa
     4561 ctgaaatgac ctaaagctac cttgtcattt ccagttaatt gaaccatata tttttgcaca
     4621 aatgctgggg aaatactcat aataataccg agtgctaatc ctcctagtaa gatgagaggc
     4681 aagctagtaa agccagcaac tgataaaatg accgcaatca tacatgccat atatagagtg
     4741 tggtgccctg ttaaaaaaat atatttaaat cgagtaaaac gagcgattaa gatattgaac
     4801 accatgcctg caaacataat cattgcagta gctgagccat atgttgttaa agctacagct
     4861 acaattgctt cattattcgg cacaacgcca gataaatgaa aagcatgctc aaacatggta
     4921 ccaaatggat ttaaagaatt ttgtacaatt cctgcaccac cagatacaac taagaaacca
     4981 acaaaggtct taattccacc tttaataata tcaggtaatt tcttcttctg aagaactaat
     5041 cctaagattg caattaaagc tactaaaata gctggtgtac taacaatatc caatatgaac
     5101 ttcatcatga cgctagcctc ctatataagt cctttttctt cacaaagttt agtaattaat
     5161 tctcgtagtt catccatatc aataatacta tttaagatac gaacatctcc aagatgacta
     5221 gctgaatcag ctagatcacg accaacaatc caaatatcag ctgcatttgg atctgctcca
     5281 cctaaatcat aatgttcaac ttctacatcc gaaacattca aatcactcaa tacagattca
     5341 atattcatct gtaccataaa acttgaacct aatcctgaac cacaagctgt accaattttt
     5401 aacattatct aatcctcctg tttaattatc attttaatgt catcatagtt ttttgatgat
     5461 attaaagttt gaacatgatt tttatctctt aaaattgttg ttaaatgtga caaagccttt
     5521 aaatgactct cattatcaat ggctgcaata caaatcaaca atcttacctc ttgttctgga
     5581 ttatccaata aataaatcgg ttcttccaaa actaacattg acattcctat ttcattcaca
     5641 ccttcatctg gccgagcgtg aggaattgct actcccttcc ctaaattaat aaaaggccca
     5701 aactcttcta ctttttgaat cattgcctca gggtagttct cagttatctt atcttgatcc
     5761 aaaagcggtt tagctgctaa acgaatcgcc tccttccatc ctaatttttg cgaactaacc
     5821 tgataggttt ctttggtaat aagttgttct agcactggta caatttcctt tctatcattt
     5881 ttttgataaa gataattctt taacgccaat cttaattcca attcttgtgt aataattcca
     5941 tatcttttga caatattcag gatttgttca atctcaaaat ccccatactc taaattcgga
     6001 aaatctttta acactagttc tactagttgt attgcttgct cttcagtcat cataaccgaa
     6061 actagataat ttggcttttc tgtctccacc tttatggtag aaaaaaccat atcatagtca
     6121 ctactagctt tcacctgtaa atcatcaatc tttgaggttc ctataaactc aatttgagga
     6181 aataatgcta atagattctc ttttaacatc aatgaagaac taacaccatt aggacaaatg
     6241 attgctgctt tataccattt ttgaggcaaa gtatctgctt tctttaaata acctccgaaa
     6301 tggataacaa aatatgctgt ttcactatca ggtatgggat tgtcaatagc atccatcaag
     6361 ggcatcaaag aatctttgac taattcaaat aaatcaggat aatgttcttt aacatgcaat
     6421 acatattcat ttgaactagg taggccgaac tttaatctat agtaagccgg tataaggtgg
     6481 cgacgaagat tttctctcaa tccttccctt tgtttaaaat gtaacaaaga aatatcttcc
     6541 attctactta taatagcctc tgttaattga ttaaagtaaa ccggagcaac atctacttca
     6601 ccttcaaagc aacttgataa taaaactgtg atatagcgat aatcatcctc agaaaacacc
     6661 gtatctataa ttcccaaatc aaccactgta tccaataaaa tagtggttat atcttgaata
     6721 acaggagata ctaatgtctc tgaaagacat actctttcaa catccctttg atacctacac
     6781 agaatgaata ctaaaccgaa aaggcaaact tttaattgat taacaatagg tactagttgt
     6841 agcttctcat aataatcttt aactacctga tttatcaaat cataagttaa tgaatacccc
     6901 caactggata aaacataatc caaaccccaa atccctatgg aggattccag caactcacta
     6961 accatttgaa aagctaagcg gtgcttattc cactctgaac cgtgtaaagt ataacctttt
     7021 gctctactgt accctagctc caaatcatta tctaacataa tctttcttaa tgattgaata
     7081 tcagataagg ttgtattctt acttactttc aaaaagtctt ggtaatgatt attcgatata
     7141 aaatctaatc ggcaaaaagt gtaaagatag attaaagcta agcgagtcga ctttggtaaa
     7201 accaattcat ccgacttaat aatatctgtc aaagactgct tcgtacgatt tgataaacta
     7261 tagcgacctt gttttttatc cagcactatc cctttattag ctagataagg cactaaataa
     7321 tctattcctt ctttgacttc ctttataggt aagctcacct taacagataa ctcatataat
     7381 gatagctcac aatgatccat caaagtcatc aaaataacta gtgctctata atcaaacatt
     7441 tctcttcctt tctaactaca attatagtgt aaaagttata agaataaaag agtatttgag
     7501 cacaatataa tctcaccttt taaaagaaat tattgggctg aaagttttta gtgattattt
     7561 ttcaactttc tattgacttt taaaaacaaa gtgatatcat ttagatatca aataatggga
     7621 ggtcattctt atgactgaaa aactaatcaa ttcaaaacca aatggtgtat tcgcattgat
     7681 tctcattgaa ttgacgctcg tacttggtat ctttatattt ataatgggtg ttggttcgga
     7741 aaacattttt ggaattatta tcggaccttt actaatcgta attgcaggtc tagctcatgc
     7801 tggtttaaaa gttgtcaaac ctcaggaagc tctggttctg acactctttg gtaattatac
     7861 aggtaccatc aaagaacctg gtttttactt tgtcaatccc ttcagcgtag cagtcaaccc
     7921 tgcaaaccac actcgacttg gacaaagtgg tgatgttagc acaaaatctc cttttttagg
     7981 tgctaaatca tcaaatgaca atgatgtaaa tcttgaaatt ggcaagaaac acatttccct
     8041 caaagtcatg accttgagca attctcgtca aaaaatcaat gattgcttag gaaaccctgt
     8101 agaaatcggt atcgcggtaa cttggagagt tgtcgatacc gctaaggcag tcttcaacgt
     8161 tgataactac aaagaatatc tttcattgca atgtgatagc gccctccgta atattgtccg
     8221 catctatcct tacgatgtgt ctcctaatgt ggatactacg ggtgacgggc aagcagatga
     8281 aggtagtctc cgtggctcta gcgaaattgt tgctaaccgt attcgtgaag aaatccaaaa
     8341 tcgtgttgag gatgctggct tggaaatcct tgaagcacgt atcacttacc tagcttatgc
     8401 tccagaaatt gctgccgtta tgcttcaacg ccaacaagca tctgccatta ttgatgcacg
     8461 gaagatgatt gtagatggtg ctgtaggaat ggttgaaatg gcactagaac ggctcaatga
     8521 aggggaattg gtagaacttg acgaagaacg aaaagctgcc atggtttcaa atctccttgt
     8581 cgttctttgt ggcaatcatg atgcacaacc aattgtcaac acaggaagtc tttactaaag
     8641 atggctgaag ctaaaaaaaa gcagatcccc cttcgactct caacaaagtt atacgctgca
     8701 ctcgcatcat gggcagaaga cgactttcgt tcagtcaatg ggcaaatcga atatctcctc
     8761 acagagtgtg tcaagcaacg gaagaaagac ggaaaatacg tatcagaaac cattgacgaa
     8821 ccatttgaga ttgatattta acaccttctc ctgtctgccg tgtcggatag gagaattttt
     8881 tcatcttttt ttgtcaagta actttacttt acaaaaaaaa tgtgttatcc tagtatggtt
     8941 gatgaaaatc agtagattga atcgaatata aatacctaaa ggagaaatca aaatggcagt
     9001 acctgcacgt cgcacttcaa aagcgaagaa aaacaaacgt cgtacacact acaaagtaac
     9061 agctccatct gtaaactttg acgaaactac tggagattac tcacgttctc accgtgtatc
     9121 acttaaagga tactacaaag gacgtaaaat cgctaaagct gcatcagctg aataatagaa
     9181 gggagatacc atgcgcgtaa atattacact tgaacacaaa gaatctggtg aacgcttgta
     9241 ccttacttct aaaaacaaac gtaacactcc agaccgcctt caattgaaga aatactcacc
     9301 aaaacttcgc aaacacgttg tgtttacaga agtgaagtaa gcattcaata cgaatcaata
     9361 cagaagaaaa acgcttgatc agcgtttttt ctttttgtta atttcactat attgcaagag
     9421 aaatcattcg aatgactaca ttttttgaaa taagaatacg gattcggaca attaaaagca
     9481 agaggaactc aaatgtaagt tggggttcct tttttgtata tttttcaaat aacgcctcta
     9541 cactatttgt agcaaattca ccaactacag ttgtatctta gttaaaataa gttagaatat
     9601 gtaagtgagt accagatata ccaagacatc gtcaccatct aaggtatatt caaaatacaa
     9661 aagttgacca actagatttc tgaatatcct tatatatcca ttcttaaaat tggtttaaat
     9721 agcgtagtct tttaaactag ttttaagaat ccaaaaaatc ttcctacata tgtaagaaga
     9781 ttttttagtt cagaatgatt agatttagct aatggatacc tatcctaccc actcaccgtt
     9841 agcattgact cgatagccat cgggtgtagt cgtgtttact aataaatctc ctgaattata
     9901 ggtatagtac catttaccag aaactgtaaa ccaaccagta gccattgaac ctgattcatt
     9961 taagtaatac cataagcctt tgtctttaac ccaaccagta gccattgaac ctgattc
//
LOCUS       DAVMGZ010000068         9954 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000068 DAVMGZ010000000
VERSION     DAVMGZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.069"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(40..804)
                     /locus_tag="VYQ93_001347"
     CDS             complement(40..804)
                     /locus_tag="VYQ93_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336025.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(900..1303)
                     /locus_tag="VYQ93_001348"
                     /pseudo
     CDS             complement(900..1303)
                     /locus_tag="VYQ93_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1483..2115)
                     /locus_tag="VYQ93_001349"
     CDS             complement(1483..2115)
                     /locus_tag="VYQ93_001349"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV3336026.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(2149..2850)
                     /gene="pyrF"
                     /locus_tag="VYQ93_001350"
     CDS             complement(2149..2850)
                     /gene="pyrF"
                     /locus_tag="VYQ93_001350"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV3336027.1"
                     /translation="MREHRPVIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            complement(3083..4291)
                     /gene="divIB"
                     /locus_tag="VYQ93_001351"
     CDS             complement(3083..4291)
                     /gene="divIB"
                     /locus_tag="VYQ93_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV3336028.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEDDKEKEEKEEKEE
                     PESKEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYAT
                     MKDIRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQF
                     PTKFTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIK
                     VFVSELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDLDEVLVPLSEMSKKLPYYSK
                     IKPQLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNR
                     NQTTQRSSRR"
     gene            complement(4301..5359)
                     /locus_tag="VYQ93_001352"
     CDS             complement(4301..5359)
                     /locus_tag="VYQ93_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV3336029.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLLELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(5363..6715)
                     /locus_tag="VYQ93_001353"
     CDS             complement(5363..6715)
                     /locus_tag="VYQ93_001353"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV3336030.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(6819..7460)
                     /locus_tag="VYQ93_001354"
     CDS             complement(6819..7460)
                     /locus_tag="VYQ93_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336031.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(7462..9483)
                     /locus_tag="VYQ93_001355"
                     /pseudo
     CDS             complement(7462..9483)
                     /locus_tag="VYQ93_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(9566..9865)
                     /locus_tag="VYQ93_001356"
                     /pseudo
     CDS             complement(9566..9865)
                     /locus_tag="VYQ93_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         2984 a         2203 c         1759 g         3008 t
ORIGIN      
        1 ataaaaaatc ttttttgtaa aattgaacaa attgtttcat tatacgtagt cctctgaatg
       61 tagaaaaaat gtaccataaa caaccaaaca actaacaaat aaaataaaag caagatgccc
      121 actaactaag gaaagactga tatctttctg atatcccaaa gctaatgttg tcacaggttc
      181 taagtaagat agccctaaaa tagcccaaaa aataccacca accatcatat aggcaactgg
      241 gatgaaaata gctcctattt ttttcttcac tagcaaagca ctagctagtc caaaaataga
      301 gaacacagcg ccccaaactc cataccagag agtcgttaca agactataga gaaactgatt
      361 ggaatcaaat aattctttta aggcaccact ataatctcca atataaattt cctgataagg
      421 agtcactaaa agattaattc ctaataataa taaatagggg agaaaaaaga ctagaaaaga
      481 agaaataatt acagtactac ccacaatagc cagatacttc tttttagaaa ttcgacacaa
      541 ttgtgctgtt agaaaatgac tctcagcatc ctctattatc tgactagaat agggcagtgt
      601 acagataagt gcagctacta ggctaatcgg tgaaaatcct cgaatagaag aagcggcaaa
      661 aaaaatcgat aaaccttcaa ttttataaat accattgaaa gcaaggaaat ttcctaaact
      721 catgcaaaga agggtcaaaa ataaagacat ataaaatcga ggtgattgaa cgactcgtac
      781 aagattacaa atgaaaaatt ccatccttac tcctccttat aataaaaata gggtgtagca
      841 ttcttttttc atgctacacc cacaatcaac catctctaag gcttactctg acaagtaagt
      901 taataagaat ctggattcca agaacctgaa gtatgaattc ttacatgatt tccaaattgt
      961 ggcgccatag ctaatctagt accagaacca atataattgt caccacctcc attatagtac
     1021 atgacaatcc tagagccaga ccccaatgaa tataccgggg taatatctga cccactatag
     1081 gcgctacgaa tagaggtatt taacctttta ccgccaccag tgctgtcatt attattaatt
     1141 ccagcagagg cgttttcttt ctcaagctca ctagttgtat ccgaacccca acgcggcaac
     1201 gttaaatcat aatctccaat ctgtctagct aaaactgtag ggagaaacaa actagatgca
     1261 attgtaacta cgcaaaaaag gaatatattt ttcttctttt catgacatcg tcctcttaag
     1321 aagtgataaa caagatagtt gattgatcaa tttgatacat attacatttt cattatatca
     1381 ataaaagaaa gcgttgtcaa ttattttatt taaaatattg tcatatttta aataaaatgg
     1441 taaaagagac tcggtagcct cgtctctctt tatcttactg acctaacctt cttgccaatt
     1501 ttcttggtct tctttaaagc gttttagaag atcaaggccc tctggcgtga tgtaaccttc
     1561 ttcttgggct agatggataa gctcgctata gtttgaaagc gtcacaagtt taacaccagc
     1621 atctgcaaag ttcttatctg cttttggcaa ttggtagctg aaaatcgcta caactccaag
     1681 tacatctgct ccttctcgct tggctgctgc tacagcttca agaactgaac cacccgttga
     1741 aataaggtct tcaaccacta ccattttttg accttgagct acgcgacctt cgatttgatt
     1801 accagctccg tggtcttttg gtttgctacg gatgtaggca aaaggcaagt ccatcttatc
     1861 agcaataatg gctccgtgtg gaatccctgc tgttgcagtt cctgcaatca cttctacttc
     1921 aggaaaggct tctttgatag cttccacaaa accattttca attagggtac gagtttctgg
     1981 ataggctagt gtcacacgat tatcagtgta aatcggtgac ttgataccag atgcccaagt
     2041 gaagggttcc tctggtttga ggtaaacggc ttggattttc aagaggtggc tagcgatatc
     2101 tttagcaagt gtcatggtat tctcctttta tttttctaat ctagttcttt aattccagtc
     2161 ctgtgtccat tcatccttga tggcatgata agctgcaaca ggatcctcag cttgggtaat
     2221 gggacgtccc actacgatat agtcactgcc gatttgatag gcatcagcag gtgtcatgac
     2281 tcgtttttga tctccaactg caacaccagc tggacgaatc cctggtgtca gacagataaa
     2341 atctggattg gtagcctgct tgatgacttg tacttcctga gccgagcaaa caacaccatc
     2401 caagccagct tcagctgtct tcttggcata gtgaatcaca gactcttgca gactggtttg
     2461 gatattttga aactcctgca tctgggcttc tgacgttgat gtcagctgcg ttacagcgat
     2521 caatttagct tgactcccaa ggccttcacg cgccgccttc atcatctcta caccaccagc
     2581 cgcatggaca ttagtcatat cgacaccaag ctgagacaag accttcatgg ctgacttgac
     2641 tgtattagga atgtcatgaa gtttgagatc caaaaagaca ctatgaccca aaccttttaa
     2701 gtaggacaca atctcaggcc ccgctgcgta ataaagctcc atccctacct tgagataaag
     2761 gctttcttct gctgggaaaa gagctaaaaa ttccttgacc gcctcaaaac taggaaaatc
     2821 aagagcaatg actggacgat gttctcgcat aggtttctcc ttttaccttt actagtgaga
     2881 gagtctggcc aacagaagcc acgaaaaaag gaacctgcca acagcaaggt tccacgaaaa
     2941 atgggtagtc tccacccttt caccgtctaa ccttagctgc ctctctggac tgctttaaag
     3001 gttttttact attctattat ttctactagc acttgtcaag taaaaacatg gtcactaaag
     3061 aactataaga gaaaaggtaa acctagcgac gcgatgagcg ctgggtcgtt tggtttcgat
     3121 tgctctcttc ctcttgtttt ttctgttctt cttcttgttt tttctcagct tccttagcct
     3181 cttgtttggc tttttcctca gcttccataa ttaatttatc cgccacagtg taactgtaaa
     3241 ttccagcttc catgtcaatc acactcggtt ctgacaattg tggcttaatc ttactataat
     3301 aaggcagttt cttgctcatt tcagaaagcg gaaccaagac ttcgtccaaa tcattcatag
     3361 tcaatcgaat taaatcggat gtcaccttgc ttggggctaa ttccaccttt tggatagccg
     3421 ccttgagttc tgggctaatt tgagcaagtt ctgagacaaa aaccttgatt tgttcactat
     3481 cattaaagag aactgataaa taagtttctg gtaaactgtt caggctcaca gaactagtct
     3541 caagctgacc actggaaaga ataggataat gattttcacc agaaatatag taggccacaa
     3601 tatcatattc cttgacctta atagtgaact tagttggaaa ttgatagaca agttgagctg
     3661 attcaaccca atagttagac ttaatctgct tttcatattt tgccttgtct agcagaaggt
     3721 taatcgtata atccgaatcc tgaatgcctg aagcctgtcg aatatcatca gctgtagttt
     3781 gcaccgttcc ctcaacacga atatctttca tggtcgcata aggactgagc aagtaggcag
     3841 agacaatcaa taaaagcaga cttggaaata aaatcgtgaa ggctcgcaag atatggatac
     3901 caggaatctt tgctttggct ggtttttcct ttgtagcctt tttagcaagc tttttatcct
     3961 gttcctcctt ctctttagac tctggttctt ctttctcttc tttctcttct ttctctttgt
     4021 catcctctga ggatgctact ttttcttcag actcttcctt agctgattct gaatcttcct
     4081 ggtctgtttc actctcctgg tcctgtttgt cctctgactt ctcagattct tctcccatcc
     4141 gagcttgtct ttccttttcc ttctcctcag ctagagccgc ttcttcttca gccttctttt
     4201 ttagatattc ttggtttcgt ttctgccatt ctgataactc tttcaattct tcgaggattt
     4261 ctttgtcctc atttttctta tcttttgaca tttactttcc ttatgataaa tcttttttca
     4321 acaattgata aaaatctgct agagatttca attccttaga agctttcatc ttagcttggt
     4381 aatcttcctt gtgacttagt aagtgagaaa gcttctcttc caaactatcc aaggtcaaat
     4441 cgctttcttg aaggtcttct gcatagcctt tttttacaaa gtaagctgca ttttcaatct
     4501 ggtcaccacg actagcttca cgaccaagcg gcacaatgac atgcaatttt gctatcgcca
     4561 agagctcaaa aatcgtattg gcaccacctc gtgtcacaac aatatcagcc aattccaaca
     4621 agggttgata gagatcggtc acatagtcaa cacgaaaaag attttggctc aactcattca
     4681 ggctagaatc tccagttaga ttgataatat tgtagcgctc tgttagttct ttcttatggt
     4741 ctgtcaccaa ttggttaaag acacgagcac ctgcagaacc gccaacaaac aatacagtcg
     4801 gcaatttatg attaaagtgg gtttgaatat ccaccaattc atctggttct ggattttttt
     4861 gatctgaaac cttggtcact gctcccacat gctcaacctt agacaaactc gaagcttgct
     4921 caaaggttga atacatctta gtcgcaaatt tataggcgat tttattggcc aagcccatag
     4981 acaggtcaga ttcgtgaata aagacaggca ctcctgacac acgcgctgcg ataacaggcg
     5041 gtactgagac aaagcccccc tttgaaaaaa gggtctgtgg acgaagtcgc aacatgataa
     5101 agagcgattg gacgattccc cagccaactt tgaagacgtc cagcatattt tgccaagaga
     5161 aatagcgacg caatttccca gtcgcaatgg agtggaaagt gacatccaaa cctgacttaa
     5221 ggatttcttg gtgttcgata ccacgcttgt ccccgatata gtggacttcc caaccatctt
     5281 cgatgaactt gggcattaac aaaagattga gggtaacgtg tccaaccgtc cccccacctg
     5341 taaagacaat ttttttcata ttttattctt ttaactccgc tactgtgtcg ataaagaggt
     5401 cgccacgtac ttcaaagtta gcatacatat cccagctggc attggcagga ctaagaagaa
     5461 ccacatctcc ttgagtcgca agctcatagg ccttgcgggt cgcatctgca atatctgtcg
     5521 cctccacata agcgacacca gccttgtctg ctgcccgttt gacacgttct gcagattgac
     5581 ccaggatgac catcttcttg agtccagtaa tgtctggcac caattcgtca aactcattgc
     5641 cacggtccaa accacctgca atcaagacga ccttgctgtt gtcaaatcct gataaggctt
     5701 tttgagtagc caagatatta gttgatttac tgtcgttata gaatttaaca cccttgatgt
     5761 catccacaaa ctggagacgg tgtttgacac caccgaaggc tgaaagagtt tccttgatgg
     5821 tttgattgtc cacatcacga agcttggcta cagcaatagt cgcaagggca ttttccacat
     5881 tgtggctacc tggaacaccg atttcattcg ctgccatgac tacttcacca cggaagtaga
     5941 gttgaccatc ttccagataa gctccatcaa ccttttcaag tgttgaaaat ggtacaacag
     6001 tggcttctgt cttggaagcc aagtctttta ccaagtcttg attaaagttc aagacaagga
     6061 aatcagctgc tgtcatcttg ttctggatat tccacttggc tgctacatat tcctcaaata
     6121 atccatgata atcgatatga gttggcatga ggttggtaat aaccgcaatc tctggatgga
     6181 attcttgaac acccatgagt tggaaagaag aaagttccat aacaagcgtg tccttatctg
     6241 atgctatttg agcaacctga ctggcaggat agccgatatt ccctgataaa agaccatgtt
     6301 gcccagcagc agtcaaaact tccccaatca tagtcgttgt ggttgtctta ccgttcgatc
     6361 ctgtgatacc aataatcggt gcttctgaaa tcaaataagc caattccacc tcagtcaaga
     6421 ctggaattcc cttggccaaa gccttttcaa tcatgggatt gctgtagggg atacctggat
     6481 tttttaccat gagggcaaac tcttcatcca agagttccaa aggatggcca cctgtaatga
     6541 ccttgatccc ttcttccagc aaacattggg cagctggatt gtcctcgaat ggtttcccat
     6601 catttactgt cacaatggca cctaacttgt ccaacaaacg agccgcagat tcaccagact
     6661 tggccaaacc taaaacaaga actttcttat ttttaaattg atctattact ttcatgtctc
     6721 gaactccatt tctactccta ctattttacc atttttatgg aaataaaaaa gccacaaagt
     6781 gtgtttgtga ctctttcttc taactgaatc ttaccatatc atctatgtga taaatcggta
     6841 actcgaatga cctgatccac ttgctcccaa atcagaggat tgtgggtcgc aataataatg
     6901 gtccgattcg gattttttaa agattctagg atggaaagta attcctcaga atttttgggg
     6961 tctaaggaag cggttggttc atctgcaaga atcaaaggcg gatcctttaa aattatcttc
     7021 gctagtgcaa cacgttgtgc ttctcctcct gataactcaa atataggttg cttcaaatcc
     7081 aaataagaga ggtttacaca gtttagagct tgtttcatca aagagatttt ctctttttcc
     7141 ttcaactttt taccaactaa acccagattg agattctctt tgacggtttg gctttcaatt
     7201 aagccaaaat cttgaaataa gtatcctaag taatctctaa agaaaacaga aggcttgatg
     7261 tccttaagag aagtgccatc atagatgatt tgccctttgt catatggctc caatcgtcca
     7321 atcatattca agagtgttgt cttaccacag ccacttgtac cgattaaggc ataaattttc
     7381 ccaccttcaa aatgaagatt cgtatctgaa aatagctgac ggcttccaaa ttttttagat
     7441 atattcttta gttcaatcat cctattttcc tttcataatt gtcatagaaa cacgagattc
     7501 tttctgcgct tgacggtaaa gcgtcaaaac tgcactagct agaaagacca ataaagtgag
     7561 caagccaatc accaagtctc gactgcttaa aataaagaga ctagcaccaa atacaaaact
     7621 ggcaaattgg ctaaccatat actgagcatg tgtttcaaaa aatcgtaaac ctgaaattcg
     7681 tttaatcaag atatctcggc ggaattgctc gaaatataga agattgacag aataaaatag
     7741 taataaggaa ctagctatcc ccacaatagc tcctaagatt aaagttgctg tttcagtttg
     7801 aacttcatta taacgagtta gataaacact tcttccttct ttaagatagg atacttgctc
     7861 ataaattcca gctttcttca agagttctag cccactctca tatcctttga taaagagttg
     7921 ttttccagca ttgatagacc aactagataa ggatataaaa ctatcacctg tagaagtcgg
     7981 cgtgaatacc actaaaatcg gatcagtcaa atactgagta gatacgggat tctcaccgtt
     8041 attataaaca aaccgctttt ctcccattga aagataacta acgtgcgctt tcatctcata
     8101 atccaaagga gcacttgcct cctcaccaga ttttccataa taactcaatc tttcttcaaa
     8161 aactttctta agttctgctt ctcgagagcg caaatgttct gggagcaaga ggataaactc
     8221 acctttttgg agatgggcta acttctgttt ggtctcagca tctaccacga ccttttcctt
     8281 gtccaaataa ctgggactaa catagagcgt attagcatct ggactatagg tatccagtgt
     8341 ctctccctgt tcattttttc cttgtggatt ggcaaaatgg agcagattat cctttacata
     8401 aagagcttgt tcctcttcga ttgcttcctt ggcaaaggca taccacttgc tctgattttc
     8461 tgtatctttt cctctatcac ctaagccaaa agaaatttga taatagtctg ctctatcctg
     8521 ccatgcttgt tttgaaattt caagttcttt caatcgttgg taagacgtca aacctgtctt
     8581 aacagcgtat cctactgtaa agacagcaac caactgacac aatagggtta aaatcatcag
     8641 gcgtttaagg ggtaatctcc ccttaataac gggaactaat gctttgtaac tcaaactcat
     8701 tagataaaga agcattagta aaattgaaat cgccaataaa aacaacagat agaaactaat
     8761 cccaaaacca taggtggcta acaagatagg ataaaacaaa ccttgactaa aaagaacgac
     8821 tcccccacct aggaaggaaa ggagggctga tagaaggagc tatttgatat cagtagataa
     8881 agaatgcccc atgatggata agagagtctg accagaaaag agttttatac ctgctgctct
     8941 catttcctta atccgagtga taatcactaa agcaaagaaa gataagccaa atattgctaa
     9001 actaattaaa ataaggggat ttagtaatat tcgaaaagca agaaaatagg gcggtatctt
     9061 tcggtcagca cttgctttat aacccaaatc tcctaattta tcggcaagct tttctttcgt
     9121 caaggagcct gacaaaagga gataactatt tagcggatca ctacgttcac gactttcttg
     9181 gctagcttct tggaattctt ttggtaaagt tccctgacca taagttgcat aagtaaagtg
     9241 agtcgtccca tccttactcg gctctacaat tcttctagct attaaactct gttctgagtt
     9301 tgcaaaattc tccaattcct gttcaaatac ctcacgcgtc ggttcctgag tatctttttt
     9361 gacacgaagt aaagaaacgg aatcatagct tgcatataaa tattgtggcg cacgtaagac
     9421 aataatccaa gcaaggaaga agctgagaaa aaaagttgat aataatatga atagtttctt
     9481 catagtagac tccttggtag actccttgta aacaaaattc cccctgtaat ttcttacaag
     9541 gggaacgatt taaatcaatg aacgattagt cataatcaca gtaaaatgct acttgttctc
     9601 cccatttagt ccaaatccat gcataggagg tttttccagc aggagcttct cctttatcag
     9661 aatgaccatc ccatctgctc acgatggatg aataatgata ttttttacca tgatagtaat
     9721 ttgaaaaagc ctaaccacct cctgaacctt ctccatatgt ccatactcct ccatctggat
     9781 attatacagc agctgatgca gctcccaata atgtaaaact tgaaataaga gctagagcaa
     9841 gtaatctatg ttttttcgtt ttcattttat tttttctttc aaaaaaatca caccttgagc
     9901 aacaatgcaa caaaataaat cctcctctct cttttattga aaccgctttc ttat
//
LOCUS       DAVMGZ010000069         9904 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000069 DAVMGZ010000000
VERSION     DAVMGZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9904
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.070"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            140..487
                     /gene="ssrA"
                     /locus_tag="VYQ93_001357"
     tmRNA           140..487
                     /gene="ssrA"
                     /locus_tag="VYQ93_001357"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(670..757)
                     /locus_tag="VYQ93_001358"
     tRNA            complement(670..757)
                     /locus_tag="VYQ93_001358"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(721..723),aa:Ser,seq:gga)
     gene            919..1659
                     /locus_tag="VYQ93_001359"
     CDS             919..1659
                     /locus_tag="VYQ93_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV3336032.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1656..2567
                     /gene="pfkB"
                     /locus_tag="VYQ93_001360"
     CDS             1656..2567
                     /gene="pfkB"
                     /locus_tag="VYQ93_001360"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV3336033.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTVEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAELIKETYGKVEVEKR"
     gene            2564..4516
                     /locus_tag="VYQ93_001361"
     CDS             2564..4516
                     /locus_tag="VYQ93_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3336034.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSAGQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4680..6905
                     /locus_tag="VYQ93_001362"
     CDS             4680..6905
                     /locus_tag="VYQ93_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV3336035.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDQDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(6950..7384)
                     /locus_tag="VYQ93_001363"
     CDS             complement(6950..7384)
                     /locus_tag="VYQ93_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336036.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNTKKEPTTSQTTTIVKEIQKESPQENTSPNKENKTSQEKTQQEETPKSSVKE
                     EKKEEQKTSTQDSSTPAPTPSKPATENEKQSNTPTSENKTNQ"
     gene            7522..8664
                     /locus_tag="VYQ93_001364"
     CDS             7522..8664
                     /locus_tag="VYQ93_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV3336037.1"
                     /translation="MIYFDNSATTKPYPEALETYVQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFSPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8673..9887
                     /gene="thiI"
                     /locus_tag="VYQ93_001365"
     CDS             8673..9887
                     /gene="thiI"
                     /locus_tag="VYQ93_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV3336038.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYAQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
BASE COUNT         2843 a         1900 c         2274 g         2887 t
ORIGIN      
        1 agtacgaaaa ttctcatctg aaagtatttt agaataattc tcttcgtgaa tttcttcaaa
       61 acagatagct tcatcttagg tatgtgattt ctttttgcat ttttgagtta gataaggtat
      121 aatggtttta ttgtcttttg gggtcgttac ggattcgaca ggcattatga ggcatatttt
      181 gcgactcgtg tggcgacgta aacgctcagt taaatataac tgcaaaaaat aacacttctt
      241 acgctctagc tgcctaaaaa ccagcaggcg tgacccgatt tggattgctc gtgttcaatg
      301 acaggtctta ttattagcga gatacgatta agccttgtct agcggcttga taagagattg
      361 atagactcgc agtttctaga cttgagttat gtgtcgaggg gctgttaaaa taatacataa
      421 cctatggttg tagacaaata tgttggcagg tgtttggacg tgggttcgac tcccaccggc
      481 tccattattc ctttgcattc ttttgcattc cttggtaaaa cgttgttaaa tcaacgtttt
      541 ttatttttat ctttggtatt cctttgcatt cttttgctaa aaagggagtc acaaacagac
      601 cctattttaa aaaaaggata gaaaaaagga tacaacattt gtcgcatcct aaaaataatc
      661 ttttttcgac ggaagacatg ggattcgaac ccacgcacgc tattacacgc ctaccgcgtt
      721 tccaacacgg cctcttaagc ctcttgagta atcttccaat acttactcaa atagtctacc
      781 ataaaggctc ttatcttgca ataaaaattc tagaaataag aaaaatgata gattttgaaa
      841 gaaaatgata aaaaatgctt gacttcgaaa gaaagtatga tagaatgaat agtgtaaacg
      901 ataacaggag gtgattcagt gttaaaaaca gaacgtaaac aactaatttt agaggagtta
      961 aatcagcatc atgtagtttc tctagaaaaa ttagttagtt tgctagaaac gtcagaatca
     1021 acggttcgaa gagacttgga tgagttggaa gcggaaaaca agcttcgtcg tgtgcatggt
     1081 ggagcagaac tcccccactc cttacaggaa gaagaaacca ttcaagaaaa atctgtcaaa
     1141 aaccttcaag aaaagaagtt gctggctcag aaagcagcct ctctcattaa agaaaaagat
     1201 gtcatcttta tcgatgctgg aacaacaact gcttttttga ttcatgaatt ggtcaataag
     1261 aatgttacag ttgtgaccaa ctccattcac catgccgctc agttggttga aaagcagatt
     1321 ccaactgtca tggttggagg aaacgtcaag acggcgacag atgctagtat cgggggcgtt
     1381 gctcttaacc agattaacca attgcacttt gaccgtgcct ttatcggaat gaatggtgtt
     1441 gacgatggct attatacgac tcctgatatg gaggagggag ctgtgaaaag agctattttg
     1501 gagaatgcca agcagaccta cgtcttggtg gattcgtcaa aaattggaca aacttgcttt
     1561 gccaaggtag caccactcaa acgcgctatc gttatcacta gtcaagggca tgagctcttg
     1621 caggttatta aggagaaaac ggaggtaata gaagtatgat ttatacagtc acactcaatc
     1681 catccattga ctatatcgtt cgtttggacc aagtcaaagt tggtagtgtc aatcgtatgg
     1741 acagtgatga taagtttgct ggtgggaaag gaatcaatgt cagccgtgtc ttgaaacgtt
     1801 tgaatatacc aaatacagcg acgggattta tcggtggctt tactggtaaa tttatcacag
     1861 atactctagc agaggaagaa atcgagacac gttttgtcca agtggcagaa gatactcgta
     1921 tcaatgttaa aatcaaagca gaccaagaaa cagaaatcaa cggaacgggt ccaactgttg
     1981 aaccggttca gctagaagaa ttgaaagcta ttttatctag tctgacagta gaagatacag
     2041 ttgtctttgc aggttcaagt gctaaaaatc taggcaatgt tatctataag gatttgattt
     2101 ccttgacgcg ccagactggt gcgcaagtgg tctgtgactt tgaaggacag accttaattg
     2161 atagtttgga ctaccagcct cttcttgtaa aaccaaacaa tcatgaactt ggagcgattt
     2221 ttggggttaa actcgaaagt ttagatgaaa ttgagaaata cgctcgtgag ttactggcta
     2281 agggtgctca aaatgttatt atctctatgg ctggggacgg tgctcttctt gtcacatctg
     2341 agggagctta ctttgctaaa ccaatcaaag gaacagtcaa aaattcagtt ggggctggtg
     2401 attctatggt tgctggattc acaggtgaat ttgtcaaatc aaaagacgca gtagaagcct
     2461 tcaaatgggg agtggcttgc ggaacggcaa ctaccttctc agatgacttg gcaacggcag
     2521 aacttattaa agaaacatat ggaaaagttg aggtagaaaa acgatgaaaa ttcaagacct
     2581 attgagaaaa gatgtcatgt tgctagattt gcaggcaact gaaaaaacag ctgtcatcga
     2641 cgagatgatt aaaaatttga cagaccacgg ttatgtaaca gattttgaaa catttaaaga
     2701 aggaattttg gcgcgtgaag ctttgacttc tactggtttg ggtgatggaa tcgcaatgcc
     2761 tcacagcaaa aacgctgctg tcaaagaagc gacagttcta tttgctaagt caaataaggg
     2821 tgttgactac gagagcttgg atggacaagc aactgacctc ttcttcatga ttgcagctcc
     2881 agaaggtgcc aatgatactc acttggcagc cttggcagaa ttgtctcaat acttgatgaa
     2941 agacggtttt gcagacaaac ttcgtcaagc aacatctgca ggccaagtta tcgaactttt
     3001 tgaccaagct tcagaaaaaa ctgaggaact tgttcaagca cctgctaatg actctggtga
     3061 ctttatcgta gctgttacag cttgtacaac aggtattgcc cacacttaca tggcccaaga
     3121 agcccttcaa aaagtagctg ctgaaatggg ggttggtatc aaggtcgaaa ccaacggtgc
     3181 tagcggtgtt ggaaatcaac taactgcaga agatatccgt aaggctaaag ctattatcat
     3241 tgcagcagac aaggccgttg aaatggatcg atttgatgga aaaccattga tcaatcgtcc
     3301 agttgctgac ggtatccgta agacagaaga gctaattaac ttggctcttt caggagatac
     3361 tgaagtctac cgtgccgcta atggtgccaa agctgcaaca gcctctaacg aaaaacaaag
     3421 ccttggtggt gccttgtaca aacacttgat gagtggtgta tctcaaatgt taccattcgt
     3481 tatcggtggt ggtatcatga ttgcccttgc cttcttgatt gacggtgctt tgggtgttcc
     3541 aaatgaaaac cttggcaatc ttggttctta ccatgagtta gcttctatgt ttatgaaaat
     3601 tggtggagct gcctttggtt tgatgcttcc agtctttgcg ggttatgttg cctactctat
     3661 tgctgaaaaa ccgggtttgg tagcaggttt cgtggctggt gctattgcca aagaaggttt
     3721 tgcctttggt aaaattcctt atgccgcagg tggtgaagca acttcaactc ttgcaggtgt
     3781 ctcatctggt ttcctaggtg cccttgttgg tggatttatc gcaggtgcct tggttcttgc
     3841 catcaagaaa tacgttaaag ttcctcgttc actcgaaggt gctaaatcaa tccttctatt
     3901 gccacttctt ggaacaatct tgacaggatt tgttatgcta gctgtgaata tcccaatggc
     3961 tgcaatcaac actgctatga atgacttcct aggcggtctt ggaggaggtt cagctgtcct
     4021 tcttggtatc gtccttggtg gaatgatggc tgttgacatg ggtggaccag ttaataaagc
     4081 agcttatgtc tttggtacag gtacgcttgc agcaactgtt tcttcaggtg gttctgtagc
     4141 catggcagca gttatggctg gaggaatggt gccaccactt gcaatctttg tcgcaactct
     4201 tcttttcaaa gataaattta ctaaggaaga acgtaactct ggtttgacaa acatcatcat
     4261 gggcttgtca tttatcactg agggagcgat tccatttggt gccgctgacc cagctcgtgc
     4321 gattccaagc ttcatccttg gttcagcagt agcaggtgga ctcgttggtc ttactggtat
     4381 caaactcatg gcgccacacg gaggaatctt cgttatcgcc cttacttcaa atgctctcct
     4441 ttacctcgtt tctgtcttgg taggagcaat cgtaagtggt gtggtttatg gttacctacg
     4501 caaaccacaa gcataaaaaa tagaaaaatg aaaagattgg accgtttggt gcagtctttt
     4561 tctcttcccg aaatgcctgt gaaatatggt ataatagaag aatggcaaac aagaatacaa
     4621 gtacaacaag acggagaccg tctaaagcag aactggaaag aaaagaagcg attcaacgaa
     4681 tgttgatttc attaggaatt gcgattttat tgattttcgc agccttcaaa ttaggggctg
     4741 caggtataac cctttataat ttaattcgct tgctagtggg tagcctagct tatctggcga
     4801 tattcggcct attaatctat ctcttctttt tcaagtggat acgaaaacag gaaggactct
     4861 tatctggctt tttcaccata tttgctggct tactcttgat ttttgaggcc tacttagttt
     4921 ggaaatatgg tttggacaag tccgttctaa aagggaccat ggctcaggtt gtgacagatc
     4981 tgactggttt tcgaacgact agctttgctg gagggggctt gatcggggtc gctctttata
     5041 ttccaacagc ctttctcttt tcaaatatcg gaacttactt tattggttct atcttgattt
     5101 tagtgggttc tctcctagtc agcccttggt ctgtttacga tattgctgaa tttttcagta
     5161 gaggctttgc caaatggtgg gaagggcacg agcgtcgaaa agaggaacgc tttgtcaaac
     5221 aagaagaaaa agctcgccaa aaggctgaga aagaggctag attagaacaa gaagagactg
     5281 aaaaagcctt actcgatttg cctcctgttg atatggaaac gggtgaaatt ctgacagagg
     5341 aagctgttca aaatcttcca cctattccag aagaaaagtg ggtggaacca gaaatcatcc
     5401 tgcctcaagc tgaacttaaa ttccctgaac aggaagatga ctcagatgac caagatgttc
     5461 aggtcgattt ttcagccaaa gaagcccttg aatacaaact tccaagctta caactctttg
     5521 caccagataa accaaaagat cagtctaaag agaagaaaat tgtcagagaa aatatcaaaa
     5581 tcttagaagc aacctttgct agctttggta ttaaggtaac agttgaacgg gccgaaattg
     5641 ggccatcagt gaccaagtat gaagtcaagc cggctgttgg tgtaagggtc aaccgcattt
     5701 ccaatctatc agatgacctc gctctagcct tggctgccaa agatgtccgg attgaagcac
     5761 caatccctgg gaaatcccta atcggaattg aagtgcccaa ctccgatatt gccactgtat
     5821 ctttccgaga actatgggaa caatcgcaaa cgaaagcaga aaatttcttg gaaattcctt
     5881 tagggaaggc tgttaatgga accgcaagag cttttgacct ttctaaaatg ccccacttgc
     5941 tagttgcagg ttcaacgggt tcagggaagt cagtagcagt taacggcatt attgctagca
     6001 ttctcatgaa ggcgagacca gatcaagtta aatttatgat ggtcgatccc aagatggttg
     6061 agttatctgt ttacaatgat attccccacc tcttgattcc agtcgtgacc aatccacgta
     6121 aagccagcaa ggctctgcaa aaggttgtgg atgaaatgga aaaccgttat gaactctttg
     6181 ccaaggtggg agttcggaat attgcaggtt ttaatgccaa ggtagaagag ttcaattccc
     6241 agtctgagta caagcaaatt ccgctaccat tcattgtcgt gattgtggat gagttggctg
     6301 acctcatgat ggtggccagc aaggaagtgg aagatgctat catccgtctt gggcagaagg
     6361 cgcgtgctgc aggtatccac atgattcttg caactcagcg tccatctgtt gatgtcatct
     6421 ctggtttgat taaggccaat gttccatctc gtgtagcatt tgcggtttca tcaggaacag
     6481 actcccgtac gattttggat gaaaatggag cagaaaaact tcttggtcga ggagacatgc
     6541 tctttaaacc gattgatgaa aatcatccag ttcgtctcca aggctccttt atctcggatg
     6601 acgatgttga gcgcattgtg aacttcatca agactcaggc agatgcagac tacgatgaga
     6661 gttttgatcc aggtgaggtt tctgaaaatg aaggagaatt ttcggatgga gatgctggtg
     6721 gtgatccgct ttttgaagaa gctaagtctt tggttatcga aacacagaaa gccagtgcat
     6781 ccatgattca gcgtcgtttg tcagttggat ttaaccgtgc gacccgtctc atggaagagc
     6841 ttgagatggc aggtgtcatt ggtccagctg aaggtaccaa accaagaaaa gttttacaac
     6901 aataaaaaaa tagcttcttt ccaagtttgg aaggaggcta ttttagtgat tattgattag
     6961 ttttattttc cgaagttggg gtattggact gtttttcgtt ttcagtagca ggtttacttg
     7021 gagtaggagc aggagtagaa gaatcctgag ttgatgtttt ctgctcttct tttttctctt
     7081 ccttgacgct ggattttggc gtttcttctt gttgtgtttt ttcttgagaa gtcttatttt
     7141 ccttattggg actagtattt tcctgagggg attccttttg aatttctttg acaattgttg
     7201 tcgtctggct tgttgtaggt tcctttttag tatttttgtt attatccaag gcgttcataa
     7261 tcgtgatact agcggtaatc aagccaagga tactagcgat agtcgcaacg gttttttgaa
     7321 agactgtaaa gccttttttg atgtgttctg tttttggttt tgaagttcta cgataattag
     7381 acatacattc tctcctttga ttatgactta ttatactctg tttctttaaa aaaatcttaa
     7441 aaaaagagaa attgcccttt cttgtcgcaa gctccgtttc atgatacaat agagggagtg
     7501 attttagaaa gcgtgagaaa catgatttac tttgataatt cagcgacgac caagccttat
     7561 cctgaagccc ttgaaaccta tgtgcaggtc gcttcaaaaa ttttaggaaa cccatctagt
     7621 ctccatcgtt tgggagacca agcaacacga atcttagatg cttctcgcca acagattgca
     7681 gatttaatcg gtaagaaaag cgatgaaatc ttctttacct ctggtggaac agaaggggat
     7741 aactgggtta tcaagggtgt ggcctttgaa aaagctcagt ttggcaagca catcattgtt
     7801 tcagccattg aacatccagc agtcaaagag tcagccctct ggttgaaaag tcaaggattt
     7861 gaagtggatt tttctccagt tgataagaaa ggcttggtcg atgttgaggc gttagcaggt
     7921 ttgatacggc ctgatacaat cctcgtttcc atcatggctg tgaacaatga aatcggctct
     7981 atccaaccta ttgaggctat ttcagaattc ttggcagaca agccgactat ttccttccac
     8041 gttgatgcgg ttcaggcgct tgccaaaatt ccgactgaaa agtatctgac agaacgggtg
     8101 gattgcgcga ccttctcgag tcataaattt catggtgtcc gtggtgttgg ctttgtctat
     8161 atcaaatctg gcaagaagat tacacctctt cttacaggtg gtggccagga gcgagattat
     8221 cgttcgacaa ctgaaaatgt ggcagggatt gcagcgacag ccaaggctct ccgtttatct
     8281 atggaaaagc tagatatctt taggagcaag actgggcaga tgaaggcagt gattcaccaa
     8341 gctcttctga actatccgga tatttttgtc ttttcagatg aggaagactt tgcacctcat
     8401 attctgactt ttggaatcaa aggtgttcga ggtgaagtca tcgttcacgc ctttgaagac
     8461 tatgatattt tcatctcaac aacctcagct tgttcatcta aggcaggaaa accagccggt
     8521 accttgattg ccatgggagt ggacaaagat aaggccaagt cagctgtgcg tcttagccta
     8581 gacttggaaa atgatatgag tcaggtcgag cagtttttga ccaagttaaa attgatttac
     8641 aatcaaacta gaaaagtaag ataggagcat tcatgcagta ttcagaaatt atgattcgct
     8701 acggagagtt gtcaaccaag ggtaaaaacc gtatgcgttt catcaataaa cttcgtaata
     8761 atatttcgga cgttttgtct atctatgccc aagttaaggt aacagcagat cgcgaccgtg
     8821 cccacgctta cctcaatgga gctgattaca cagcagttgc agaatctctc aaacaagttt
     8881 ttggaattca aaacttttct cctgtttata aggttgaaaa atctgtagaa gttttgaagt
     8941 cttctgtcca agagattatg cgggacatct acaaggaagg tatgaccttt aagatttcta
     9001 gcaagcgtag cgaccacaac tttgaacttg atagtcgtga actcaaccaa acacttggag
     9061 gggctgtatt cgaagccatt ccaaatgtgc aagttcaaat gaaaagtcct gacatcaatc
     9121 ttcaggtgga gattcgtgaa gaagcagcct atctttctta tgaaaccatt cgtggggctg
     9181 gtggtttgcc agttggaact tcaggtaaag ggatgctcat gttgtcagga gggattgact
     9241 cacctgtagc aggttatctt gctcttaagc gtggggtgga tatcgaggca gttcactttg
     9301 ctagtccacc atatactagt cctggtgccc tcaagaaagc gcaggacttg acacgtaaat
     9361 tgaccaagtt tggcggaaat atccagttta tcgaggtgcc tttcacagag attcaagagg
     9421 aaatcaaagc caaagcgcca gaagcttatt tgatgactct aactcgtcgc tttatgatgc
     9481 ggattactga ccgtattcgt gaggtacgaa atggtttggt tatcatcaat ggggaaagtc
     9541 taggtcaagt agccagccaa acccttgaaa gtatgaaggc tatcaatgct gttaccaaca
     9601 ctcccatcat tcgtcctgtg gttaccatgg acaagttgga aatcattgac atcgcccagg
     9661 aaatcgatac ctttgacatt tcaatccaac cgtttgaaga ctgttgtacc atttttgcac
     9721 cagatcgtcc aaaaacaaat cctaaaatta agaatgcgga gcagtacgaa gcgcgtatgg
     9781 atgttgaagg cttggttgag cgagcagtgg ctggaatcat gattactgaa atcacacctc
     9841 aagccgaaaa agatgaagtt gatgacttga ttgataatct actctaattc agaaaatcca
     9901 aaag
//
LOCUS       DAVMGZ010000070         9870 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000070 DAVMGZ010000000
VERSION     DAVMGZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.071"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            21..884
                     /locus_tag="VYQ93_001366"
     CDS             21..884
                     /locus_tag="VYQ93_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3336039.1"
                     /translation="MSTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIQAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELGTV"
     gene            1267..1887
                     /locus_tag="VYQ93_001367"
     CDS             1267..1887
                     /locus_tag="VYQ93_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336040.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYTGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1973..2416
                     /gene="tsaE"
                     /locus_tag="VYQ93_001368"
     CDS             1973..2416
                     /gene="tsaE"
                     /locus_tag="VYQ93_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV3336041.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2406..2924
                     /locus_tag="VYQ93_001369"
     CDS             2406..2924
                     /locus_tag="VYQ93_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3336042.1"
                     /translation="MEYELLIREAEPKDAAELVTFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLID"
     gene            2932..3948
                     /locus_tag="VYQ93_001370"
     CDS             2932..3948
                     /locus_tag="VYQ93_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV3336043.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4033..5289
                     /locus_tag="VYQ93_001371"
     CDS             4033..5289
                     /locus_tag="VYQ93_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV3336044.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5344..6510
                     /gene="recA"
                     /locus_tag="VYQ93_001372"
     CDS             5344..6510
                     /gene="recA"
                     /locus_tag="VYQ93_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV3336045.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            6817..8187
                     /locus_tag="VYQ93_001373"
     CDS             6817..8187
                     /locus_tag="VYQ93_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV3336046.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8563..9519
                     /gene="lytA"
                     /locus_tag="VYQ93_001374"
     CDS             8563..9519
                     /gene="lytA"
                     /locus_tag="VYQ93_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV3336047.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
BASE COUNT         3015 a         1682 c         2187 g         2986 t
ORIGIN      
        1 aggaaggttt attatcttta atgagtacac tcgcagagaa attcagattg aaaagaaaag
       61 agttgagact ctcccaacaa actcttgcag aaggaatttg tgaacaaagc cagattagta
      121 aaattgagag agggcatttc attccctccg cagacctttt gttcaaactc tctcaacgac
      181 ttgaagtacc attagattat ttttttaatg aacaaattga aattaaatct aacctctcta
      241 atttcaagca attgtctgct cgactattag acgatagaaa ttatgacgat ttggaatata
      301 tttatagaat agagattgaa cgaagtactt ttctaacact agaagaccga acttaccttg
      361 aatggattca agctattatt gacttctatc aatatgacag taagtgtgag gcaatttctt
      421 cattggaaaa tatattatta aaagtctctt caaatactct gatttattta aaggcgttga
      481 atactctatc taatttctat tccttagtgg gtcgtgaaca agaatatgag gcaaactact
      541 ctcatttaat agagttgtat cagacaaaaa atttggacca tcaagagttt ttatttggct
      601 acatcagagt tcgttacaac tacgctcact acctagtatc aaaggaaaaa tataatgaag
      661 ccatccaaga agctcttgaa acgattgaac tctgtaaaca aagacagaca agctaccaat
      721 tggctcctct acttattctt gtaggaaatg ctggagccaa atttctagac agagaacaag
      781 tcaaaaatta ttatatagaa gcaagagagt tatgtaagat ttataacaat cctttaatgt
      841 tgatgaagat agaaaattat ttgaaggaac taggtactgt ttagttaatc ttgacattat
      901 aatttttctg aaacgttaaa taacctgtaa ggctgatagt tagattaata ctatcagcct
      961 tataattatg tgactctgag tccgtctcgc tccgtgaggt gcgagacaaa acaaccaccc
     1021 gtttgacgga ttttcatgat ttcaaattta tattcttcga aaatctcttc aaatcacgtc
     1081 agctctatct gcaatctcaa aacactgttt tgagcaacct gtggctagct acctagtttg
     1141 atctttgatt ttcattgagt attagtctct agtgtttgtt tagcgtctta aagagacatt
     1201 tgagaagatt agaaacagtt ctctcttttc tcctagtata atgattggta ggaagggaga
     1261 gagaagatga aatcaatgag aatcttattt ttgttagctt taattcaaat cagtttgagt
     1321 agctgtttcc tatggaagga atgcatcttg tcctttaaac aaagtacagc ttttttcatc
     1381 ggaagcatgg ttttcgtttc aggaatctgt gctggagtaa attatcttta tacccgtaag
     1441 caagaagtcc atagtgtcct agccagtaag aagtcggtga agctttttta cagtatgtta
     1501 ctcttaatta atttgttagg agctgttctt gttttgtcag ataacttgtt catcaaaaat
     1561 acgctgcagc aagaattagt tgacttttta ttgccatcct tctttttcct atttgggcta
     1621 gatttgctga tttttttacc cttgaaaaaa tacgtgcgcg attttcttgc tatgctagac
     1681 agaaaaaaga cagtgttggt gactatttta gcaacacttc ttttcttaag aaatccaatg
     1741 accattgtct cacttctgat ttatactgga ctgggcttgt tttttgcagc ctatcttgtc
     1801 ccaaattcgg ttaagaagga agtttccttt tatggtcata ttttccgaga tcttgtattg
     1861 gtcattgtta cgctcatttt cttttagaga aagcctgttt tttatggatg ttatgatgat
     1921 aagtttagta ggaaaataga cacaaaagaa aagtttttgg tataataaga gtatgtacac
     1981 aaaaaatgaa gaagagttgc aagccttagg ggagcgtttg ggccatctat tagcaaagaa
     2041 tgatgtttta atcttaactg gagaactggg tgcaggtaaa acgaccttta ctaaaggact
     2101 tgcaaaagga ttacagattt ctcaaatgat taaaagtccc acctatacta tcgtgagaga
     2161 gtatgaaggt cgacttccac tttatcacct agatgtttat cgtattgaag gagatgctga
     2221 ttctatcgac ttggatgagt ttatctttgg tggcggcgtg actgttattg agtggggaaa
     2281 tctcttagga gatgccttgc cagatgctta tttagaattg gaaattctaa aagaagcaga
     2341 tggacgccgt ttaaattttc aggcaaaggg tttgcgtgct gagaaattgt tagaggagct
     2401 tcaatatgga gtatgaattg ctcattaggg aagcagagcc caaagatgca gctgaattag
     2461 tgaccttttt aaatcgtgtg agtttggaga cagactttac cagcctagac ggagatggta
     2521 ttctcttgac tagtgaggag atggaaatat tcctcaacaa gcaagctagt tcggacaatc
     2581 agataacctt acttgcattc ttaaatggca aaattgctgg tattgtaaat attacagctg
     2641 atcagcgtaa gagagtccgt catattggag atctcttcat tgtgattgga aaaagatatt
     2701 ggaataatgg cttgggaagt ttgttgctag aagaagcgat agagtgggca caagcaagtg
     2761 gcattctgcg tcgtctccaa ctgactgtcc aaactcgtaa tcaagcagca gtccatcttt
     2821 atcaaaagca tggctttgtc attgaaggta gccaagagcg tggggcatat atagaagaag
     2881 ggaaatttat cgatgtttac ttgatgggta aactgataga ttagtagaaa tatggttaaa
     2941 aaaattattg gaatggtgct agctttactt tctgtaactg tagtaggagt aggtgttttt
     3001 gcttatacta tttatcaaca agggacagaa accttagcta aaacctataa aaaaatcggc
     3061 gaagaaacca aggttattga agcgactgaa cctctaacca ttctgttaat gggagtggac
     3121 accggaaatg ctgaacgaac tgaaacttgg gtcggtagaa gtgatagcat gatcttgatg
     3181 acagtgaatc ctaaaacgaa aaaaacaaca atgatgagtt tagagcggga tattctgacg
     3241 cgcattgaat cagggaatgg tcaggctcat gaagcgaaac tgaactcagc atatgcagat
     3301 ggtggagcag agcttgctat agaaaccatt caaaaaatga tgaatatcca tattgatcgc
     3361 tatgtgatgg tcaatatgag agggttgcaa aaactagtgg atgcagtagg aggtattaca
     3421 gtcaataata tcctaggttt cccaatttct atcagtgacc aagaagaatt taatactatt
     3481 tctatcggtg ttggggagca acatattggg ggagaagaag ccctagtcta tgcacgaatg
     3541 cgttaccaag atcctgaggg ggattatggt cgtcaaaaac gtcaacgtga agttattcaa
     3601 aaagtcatgg aaaaagctct cagtttaaat agcgttggtc attatcaaga gattctaaaa
     3661 gctttgagtg acaatatgca gaccaatatt gatttgtctg caaaaagtat ccctaacttg
     3721 ctaggctata aagattcatt taaaaccatt gaaactcagc agttgcaggg tgaaggagag
     3781 atacttcaag gtgtttctta ccagattgtt tcgagagcac atatgttgga aatgcaaaat
     3841 atactccgac gttctttggg acaagaagaa gttactcagc ttgaaaccaa tgcggtttta
     3901 tttgaagatt tatttggcag agcacctgtt ggtgatgaag ataattaact tttgatacaa
     3961 ataaaaaaat caatcgtagg aaaattcctg cgattttttc aaaaaaatac gaatagatag
     4021 gtaggaggaa acatgaaagc agaaatcatt gctgttggaa cagagatttt gacaggacag
     4081 attgtcaaca ccaatgccca gtttttgtcg gaaaaactag ctgagattgg ggtagatgta
     4141 tattttcaga cggctgtagg agacaatgaa gttcgtctct tgtctttgct tgagattgcc
     4201 agtcaacgta gcagtctggt gattttgaca ggcggtttgg ggccaactga ggacgaccta
     4261 accaaacaaa ccctagctaa atttttaggg aaagcattag tctttgatcc tcaggctcag
     4321 gagaagttgg atatcttttt tgccctgcga ccagactatg cccgaacacc aaataacgaa
     4381 agacaagctc aaattgtaga aggagcgatt ccactgccaa acgaaacagg actggctgtt
     4441 ggaggaaaat tagaagtaga cggagtgacc tatgtcgtcc ttccaggtcc gccaagtgaa
     4501 ttgaaaccca tggtcttaaa ccaacttcta cccaagttga tgacagggag caagctgtat
     4561 tcccgagttc ttcgtttctt tgggattggc gagagccagt tggttacgat tttggctgat
     4621 ttaattgata atcagatcga tcctaccttg gccccttatg ccaagacagg agaagtcact
     4681 ctacgtctgt caacaaaggc tagcagtcaa gaagaggcga atcaagcgct ggatatcttg
     4741 gaaaatcaaa tcttggactg ccagactttc gaaggaattt ctttacgaga cttttgctat
     4801 ggttatgggg aagaaactag tttagccagc attgtggtag aagaactgaa aaggcaaggg
     4861 aaaaccatcg cggctgcaga gagtttgacg gcaggtcttt tccaagctac cgtggcgaat
     4921 ttttctggag tttcaagtat atttgagggt ggttttgtga cctatagctt ggaggaaaaa
     4981 tcaaggatgt tggatattcc tgccaagaat ttggaagaac atggtgtggt gtctgaattt
     5041 acagctcaga agatggctga gcaggcacga agcaagaccc agtctgattt tggaattagt
     5101 ttgactggag tggcaggacc agatagccta gaaggacacc cagttgggac agtcttcata
     5161 ggcttggcgc aagatcaagg aactgaggtt atcaaggtta atattggagg cagaagccga
     5221 gcagatgtac gtcacattgc ggttatgcat gcctttaacc tagttcgcaa ggctttatta
     5281 agtgactaac ttttgatata atagtagata ggtctgagga tcattagaat gtaggagaat
     5341 agaatggcga aaaaaccaaa aaaattagaa gaaatttcaa aaaaatttgg ggcagaacgt
     5401 gaaaaggcct tgaatgacgc tcttaaattg attgagaaag actttggtaa aggatcaatc
     5461 atgcgtttgg gtgaacgtgc ggagcaaaag gtgcaagtga tgagctcagg ttctttagct
     5521 cttgacattg cccttggctc aggtggttat cctaagggac gtatcatcga aatctatggc
     5581 ccagagtcat ctggtaagac aacggttgcc cttcatgcag ttgcacaagc gcaaaaagaa
     5641 ggtgggattg ctgcctttat cgatgcggaa catgcccttg atccagctta tgctgcggcc
     5701 cttggtgtca atattgacga attgctcttg tctcaaccag actcaggaga gcaaggtctt
     5761 gagattgcgg gaaaattgat tgactcaggt gcagttgatc ttgtcgtagt cgactcagtt
     5821 gctgcccttg ttcctcgtgc ggaaattgat ggagatatcg gagatagcca tgttggtttg
     5881 caggctcgta tgatgagcca ggccatgcgt aaacttggcg cctctatcaa taaaaccaaa
     5941 acaattgcca tttttatcaa ccaattgcgt gaaaaagttg gagtgatgtt tggaaatcca
     6001 gaaacaacac cgggcggacg tgctttgaaa ttctatgctt cagtccgctt ggatgttcgt
     6061 ggtaatacac aaattaaggg aactggtgat caaaaagaaa ccaatgtcgg taaagaaact
     6121 aagattaagg ttgtaaaaaa taaggtagct ccaccgttta aggaagccgt agttgaaatt
     6181 atgtacggag aaggaatttc taagactggt gagcttttga agattgcaag cgatttggat
     6241 attatcaaaa aagcaggggc ttggtattct tacaaagatg aaaaaattgg gcaaggttct
     6301 gagaatgcta agaaatactt ggcagagcac ccagaaatct ttgatgaaat tgataagcaa
     6361 gtccgttcta aatttggctt gattgatgga gaagaagttt cagaacaaga tactgaaaac
     6421 aaaaaagatg agccaaagaa agaagaagca gtgaatgaag aagttacgct tgacttaggc
     6481 gatgaacttg aaatcgaaat tgaagaataa gctgttaaag cagtggagaa atccgctact
     6541 ttttcgattt ttgattcaag tttttagatt atatatagta gcttgaaata agatatgaac
     6601 aactttatta ggaaagtcaa attaatttct agaaatgttt tagcagctac agcgtactat
     6661 tccaaactca accaactata atagatcgaa actagaatag tacacatcta cttctaaaac
     6721 attgttaaaa atcgatttga ctgtccttat ttcattccgc tatatataag tttgctgttt
     6781 cttgtcgctc ctctggaaag ctgatataat agctttatga ataaaaaacg aacagtggac
     6841 ctgatacatg gtccgattct tccctcgctc ttaagcttca cctttccaat cttgctatca
     6901 aatatttttc aacagctcta taacactgct gatgtcttga ttgttggacg atttcttggt
     6961 caagaatcct tggctgcagt aggagcgacg acagcgattt ttgacctgat tgtaggtttt
     7021 acacttggtg ttggcaatgg catggggatt gtcattgctc gttattatgg ggctcggaat
     7081 ttcactaaaa tcaaggaagc agtagcagcc acctggattt taggtgctct tttgagcatt
     7141 ctagttatgt tgctgggctt tcttggcttg tatcctctct tgcaatactt agatactcct
     7201 gcagaaattc ttcctcaatc ttatcaatat atttctatga ttgtgacctg tgtaggtgtc
     7261 agctttgctt ataatctttt tgcaggcttg ttgcggtcta ttggtgacag tctagcagcc
     7321 ctgggatttc tgattttctc tgccttggtt aatgtggttc tggatctcta ttttattacg
     7381 caattgcatc tgggagttca atccgcagga cttgctacca ttatttcgca aggtttatca
     7441 gcggttctct gcttttatta tattcgtaaa agtgtgccag aactcttgcc acagtttaaa
     7501 catttcaaat gggacaaaag cttgtacgcg gatctcttgg agcaaggttt ggctatgggc
     7561 ttgatgagtt caattgtatc tatcggtagt gtgattttac agttttctgt taatacattt
     7621 ggtgcagtga ttattagtgc ccagacggca gctcgacgca ttatgacctt tgcccttctt
     7681 cctatgaccg ctatttctgc atcaatgacg acctttgctt ctcagaatct aggagctaag
     7741 cgacctgacc gcattgttca aggtcttcga atcggcagtc gtttaagtat atcctgggca
     7801 gtttttgttt gtattttcct cttttttgcc agtccagcct tggtttcctt cttggctagt
     7861 tcgacagatg gttacttgat agaaaatgga agtctctatc tgcaaatcag ttcagccttt
     7921 tatcccattt tgagcctctt gttgatttat cgcaattgct tgcagggctt ggggcagaag
     7981 atccttcctc tagtttctag ctttattgaa ctaatcggaa aaatcgtttt tgtggttttg
     8041 attattcctt gggcaggata taagggtgtt atcctttgtg aacctcttat ctgggttgcc
     8101 atgacagttc aactgtactt ctcactattc cgtcatccct tgataaaaga aggaaaggca
     8161 attttggcaa ccaaagtgca atcctagttg gatttactga ataaaatcca tttcctctag
     8221 tgaacatcga aaaaacttgt gttctcttct ttagtttggt gttgaaaata gtttaacaga
     8281 cttttgactt cttttatatg atataataaa gtatagtatt tatgaaaagg acatatagag
     8341 actgtaaaaa tatacttttg aaaagctttt tagtctgggg tgttattgta gatagaatgc
     8401 agaccttgtc agtcctattt acagtgtcaa aatagtgcgt tttgaggttc tatctacaag
     8461 cctaatcgtg actaagattg tcttctttgt aaggtagaaa taaaggagtt tctggttctg
     8521 gattgtaaaa aatgagttgt tttaattgat aaggagtaga atatggaaat taatgtgagt
     8581 aaattaagaa cagatttgcc tcaagtcggc gtgcaaccat ataggcaagt acacgcacac
     8641 tcaactggga atccgcattc aaccgtacag aatgaagcgg attatcactg gcggaaagac
     8701 ccagaattag gttttttctc gcacattgtt gggaacggtt gcatcatgca ggtaggacct
     8761 gttgataatg gtgcctggga cgttgggggc ggttggaatg ctgagaccta tgcagcggtt
     8821 gaacttattg aaagccattc aaccaaagaa gagttcatga cggactaccg cctttatatc
     8881 gaactcttac gcaatctagc agatgaagca ggtttgccga aaacgcttga tacagggagt
     8941 ttagctggaa ttaaaacgca cgagtattgc acgaataacc aaccaaacaa ccactcagac
     9001 cacgttgacc cttatccata tcttgctaaa tggggcatta gccgtgagca gtttaagcat
     9061 gatattgaga acggcttgac gattgaaaca ggctggcaga agaatgacac tggctactgg
     9121 tacgtacatt cagacggctc ttatccaaaa gacaagtttg agaaaatcaa tggcacttgg
     9181 tactactttg acagttcagg ctatatgctt gcagaccgct ggaggaagca cacagacggc
     9241 aactggtact ggttcgacaa ctcaggcgaa atggctacag gctggaagaa aatcgctgat
     9301 aagtggtact atttcaacga agaaggtgcc atgaagacag gctgggtcaa gtacaaggac
     9361 acttggtact acttagacgc taaagaaggc gccatggtat caaatgcctt tatccagtca
     9421 gcggacggaa caggctggta ctacctcaaa ccagacggaa cactggcaga caagccagaa
     9481 ttcacagtag agccagatgg cttgattaca gtaaaataat aatggaatgt ctttcaaatc
     9541 agaacagcgc atattattag gtcttgaaaa agcttaatag tatgcgtttt tttgtggaga
     9601 tatttccttc aatgtttgct actatattaa acaaaaatca gaaagcaaac tagaaagtta
     9661 tgctcaaata aaatctaaat ttgacaatgt aaaccgagtc ggatagcttt aagtactgtt
     9721 ttgaggttga agatacgatt tttgatagga actcatcaat tttagatttt taagcagcat
     9781 caataaattg cttccttgtt ttgtcataat ttttttattt aaaaaattat gacaagagtg
     9841 tgctattctt tttatgagag gtgtatgaat
//
LOCUS       DAVMGZ010000071         9820 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000071 DAVMGZ010000000
VERSION     DAVMGZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.072"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            229..528
                     /locus_tag="VYQ93_001375"
     CDS             229..528
                     /locus_tag="VYQ93_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336048.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIVDGKAVEVTLREPYSRDLLKVVKSIKQRQKLIAYRYKEGKLLFVKKEASDVL"
     gene            518..1006
                     /locus_tag="VYQ93_001376"
     CDS             518..1006
                     /locus_tag="VYQ93_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336049.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVKSLLKILEWSRRQALAQDSAYK
                     IGVQKLEELLQSPYAIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFY
                     AKDRSILDNHLDHLLEKMVNDPEKLADFTMDKSLDDAIDEAKSQITFRQEGAVKQKEM
                     VR"
     gene            1006..2883
                     /locus_tag="VYQ93_001377"
     CDS             1006..2883
                     /locus_tag="VYQ93_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEV3336050.1"
                     /translation="MYSGKKFLLFSLLGILLGYLFHRLTLLYDSYTGNTLDKWTHLLM
                     EGQDEVLQSPWNVSFTGKSSAFFLLGFVMMLLVYLYLETGKKQYREGVEYGSARFGTL
                     KEKKLFYGKEFSHDTILAQDVRLTLLDKKPPQYDRNKNIAVIGGSGSGKTFRFVKPNL
                     IQMNSSNIVVDPKDHLAEKTGKLFLEHGYQVKVLDLVNMKNSDGFNPFRYIETENDLN
                     RMLTVYFNNTKGSGSRSDPFWDEASMTLVRALASYLVDFYNPPKTREQLIEESRLSQK
                     EYQNLLKRQKKEVEERKKRGRYPSFAEISKLIKHLSKGENQEKSVLEILFENYAKKYG
                     TENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNIQSVMDLTKRDTLDMKTWGKEKS
                     MVYLVIPDNDSTFRFLSALFFSTVFQTLTRQADIDFKGQLPLHVRVYLDEFANIGEIP
                     DFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLVYLGGNDEDTFK
                     FMSGLLGKQTIDVRNTSRSFGQTGSGSLSHQKIARDLMTPDEVGNMKRHECLVRIANM
                     PVFKSKKYNSTKHPNWKYLANQESDERWWNYQINPLNQRQENYLDDLRIRDLTFESSL
                     K"
     gene            2904..3146
                     /locus_tag="VYQ93_001378"
     CDS             2904..3146
                     /locus_tag="VYQ93_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336051.1"
                     /translation="MITHFKGFVYGVDASAMFAQAMSLLQKGLIAVGAFLVVVGIVNL
                     ATNIKDGGPGVRNAILEIVGGVMVGAAGAFVTQISI"
     gene            3163..4017
                     /locus_tag="VYQ93_001379"
     CDS             3163..4017
                     /locus_tag="VYQ93_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HEV3336052.1"
                     /translation="MNLSLVSPFVYLASEKISAENLFEGFNVDLQSTVDLIKSLSSYN
                     PTVWTYMSSITKSVMQPLGVAILSVVLILEFSKMAKKIANSGGAMTFEALAPMLISYI
                     MVAVVITNTTVIVEAIIGIASHAIEQVASIVAHGGAKYDTLSGLKGSGFIGRMIVGFF
                     ALLIWLVRIVSAAMVNLLVSIRFIQLYLMIPFAPLTIPTFLSDEWKSIGIGYLKNIMV
                     YAVQGVLIFLIVSLVPLFESAGKIAVSNGAGVLQSLAIMFGSLVQAILLIIALVGSQR
                     TARSILGM"
     gene            4071..4430
                     /locus_tag="VYQ93_001380"
     CDS             4071..4430
                     /locus_tag="VYQ93_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEV3336053.1"
                     /translation="MNTCVFKDISKYQHRAWLGFTTRQIIFVLPAFIVTIIVLGLNLF
                     FWQFGDWFVYGFVFAFTIPLMLFGVYKPNDLYFEHYLKYRLHFELTVPLRTITGKKGP
                     EHEKKIKYIKETKNFND"
     gene            4381..6738
                     /locus_tag="VYQ93_001381"
     CDS             4381..6738
                     /locus_tag="VYQ93_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV3336054.1"
                     /translation="MKRKSNTLKKQKTSMTNKKEEVKGKKEEVLPSTANTLSYQALYQ
                     NGLMQVKEDYFSQSYLLGDVNYQTVGLEDKGAIIEKYSDLINSLDDQTNFQLTIFNKR
                     LNLEKFRQSVLYEEKEDGYDSYRKELNRMMNQNLDSGENNFSAVKLISFGRKDSNPKQ
                     AYRSLSQIGEYFKSGFSEIDARFESLAGEERVNLLADMLRGEHHLPFSYRDLTRSGQT
                     TRHFIAPNLLDFKNKNYLQINDRLLQIVYVRDYGMELGDQFIRDLMQGDLELIVSLHA
                     QSSTKADAMKKLRTKKTLMESQKIGEQQKLARTGIYLEKVGHVLESNIDEAEELLKTM
                     TETGDKLFQTVFLIGVFGQDEEELKQALDTIQQVAGSNDLMIDKLPYMQEAAFNSLLP
                     FGCDFLEGVSRSLLTSNVAVNSPWTSVDLQDRSGKYYGINQISSNIITIDRSLLNTPS
                     GLILGTSGAGKGMATKHEIITTKIKESGENTEIIIVDPEAEYSVIGRTFGGEMIDIAP
                     DSQTYLNVLDLSEENMDEDPVKVKSEFLLSFIGKLLDRKMDGREKSIIDRVTRLTYQS
                     FKEPSLEEWVFVLSQQAEEEAQNLALDMELYVEGSLDIFSHKTNIQTGSNFLIYNVKK
                     LGDELKQIALMVVFDQIWNRVVRNQKLGKKTWIYFDEMQLLLLDKYASDFFFKLWSRV
                     RKYGASPTGITQNVETLLLDPNGRRIIANSEFMILLKQAKNDREELVQLLGLSKELEK
                     YLVNPEKGAGLIKAGSVVVPFKNKIPQGTQLFDIMSTDPDKMASN"
     gene            6750..9563
                     /locus_tag="VYQ93_001382"
     CDS             6750..9563
                     /locus_tag="VYQ93_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV3336055.1"
                     /translation="MKDKREIIRARKAFRRSLKDEKKFLKKGKKEVRKQKKDSAVLDE
                     KAWKKEIKEKLEEMREASKARVKQANEDYNHILQNTPPSLLNRKELRDRRLPHARKRL
                     KIAKKQFREAKVEAKEERKESRKERKTNQKFLYGQESKAKSNFFFQGKSLEELKAKKE
                     VKAAKDNLKSTKQAYKSKKVSRKAKTFLYVLGREGGELASENEDLEGYRTLQETIRKG
                     KRYSRLSYNLGKASVKTGQATGRFTKKRLTNTKERYHHFKDGKGWKLAKDNPSSFKNR
                     YRKLKKQGLSSVRNIYQKLKAAFSFFTFTAGNPATWIVGGLVFLLLLMMSFFLGFSSA
                     SLIQQDEFELTKAYTHLTWEDAEHTRTNDKGITYYTKVDDVMGYMNFKFHDYELHKPV
                     HLFSSETYKDYLSTLWHDLNDGDDLKSMQDLYETPKYKLSKDDQEEMKELKEEGVYAS
                     MQELDNPFEGKSNEDSLTMTYRYGYYDLDGKPTLQEYILLEAKAHQTIVAPMDGVVSL
                     DGDNIILTNGKGENESRLTLYSIHNGRAIEGTRVLTGDIIGETPDDTGLKVSYQKYKN
                     KKEKLVYVNPQFYFPKVIQLQTTILPAIGQFGGDEFERAKHIYEFLKSQGASPQAIAA
                     ILGNWSVESSINPKRAEGDYLSPPVGATDSSWDDETWLAIGGPAIYSGAYPNILHRGL
                     GLGQWTDTADGSTRHTALLNYAHIKNKKWYDLDLQLDFMLHGDSPYYQSWLKDFFGNT
                     GSAANLAQLFLTYWEGNSGDKLLERQTRATEWYYQIEKGFSQTNGGQAKSDPQSLEGV
                     RGDLYDHSVPGGGDGMAYAYGQCTWGVAARMNQLGLKLKGSNGEKISIINTMGNGQDW
                     VATASSLGGETGSTPRAGAIVSFVGGTHGTPAIYGHVAFVEKVYDDGSFLVSETNYGG
                     NPNYTFRKISQADSAISFAYTVK"
     gene            complement(9598..>9820)
                     /locus_tag="VYQ93_001383"
     CDS             complement(9598..>9820)
                     /locus_tag="VYQ93_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336056.1"
                     /translation="KLSGQELFEIVIQKIEEFRAIVGTQMVFLDSINHPKVIQFYEQF
                     GFVAYSQLIKDDHQVSYQPMALNMSLYKK"
BASE COUNT         3251 a         1629 c         2044 g         2896 t
ORIGIN      
        1 taaaaaatgt gataaaaatt tcaactactc ttctctataa taaagctacc tgttctttga
       61 aaattgaatc cactccgtct tgattagcgt gcctgtgtaa gtagggactg agagtatttc
      121 cctgtgaagg gcaactggca caagacgtgt gtgagaattt tctaacagac acggagttta
      181 tcgttaccag acttaaacga tgcgacaaac tagataaagg agttaatcat gaaggtagta
      241 aacttgtatg atttgaaaca aatgggaaat aaaggtggtt gtaccatcca attgattcat
      301 cactttcctt ttggtatggg cttaggacat ctcaaaaaag actacattga atttaaacgt
      361 gttggtatcg ttgatgggaa ggcagtagaa gtcactcttc gagaacctta ttcgagagat
      421 ctactgaagg ttgtcaagtc gattaagcaa cgtcagaaat tgatagctta tcgttataaa
      481 gaaggaaagc tgctatttgt gaagaaggag gcatcagatg tcctctgaac aacaggaacg
      541 aatggcagtt caatatgcag agcgtagtct tttatttact gtaaaaagtc ttttaaagat
      601 tctagaatgg tctagacgtc aggctttagc acaggattcc gcctataaga taggagtgca
      661 gaaattagaa gaattgctac aatctcccta tgcgattgat acgattaatc tgaaaaaaga
      721 ttttttagac aaaccaattg atatagagaa atttaaagct tttttagaaa aagaagagat
      781 tcctttagcc atcgcttggc aaggggattc tctacatttc tacgcgaaag accgttcgat
      841 tttagacaat catttagacc atcttttaga aaaaatggtt aatgatccgg agaaattagc
      901 tgattttacc atggataagt cattagatga tgctattgat gaagctaaat cccaaattac
      961 ctttagacaa gaaggagccg tcaaacagaa agagatggtg agatgatgta cagtggaaaa
     1021 aaattcctac tattctcact gttaggtatc ttactaggct atctttttca tcgtttgacg
     1081 cttttgtatg attcctatac tggaaatacc ttagataaat ggactcatct tctgatggaa
     1141 ggtcaagatg aagttcttca gtcgccatgg aatgtttctt ttactggaaa atcaagtgct
     1201 ttttttctac taggttttgt gatgatgtta ctggtttatc tctatctaga gactggtaaa
     1261 aaacaatacc gagaaggggt agaatacggg agcgcccgtt ttggaactct aaaagaaaag
     1321 aagctctttt acggtaagga attttctcat gatacgatct tagcacaaga tgttcgtttg
     1381 acattattag ataaaaaacc accccaatat gatagaaata agaatattgc ggtgattgga
     1441 ggttcaggaa gtgggaagac atttcgcttt gtgaaaccca atctgattca gatgaatagt
     1501 tctaatattg tagtggatcc taaagatcac ttggccgaga aaacaggcaa actcttttta
     1561 gaacatggct accaagtaaa ggtgttagat ttagtcaata tgaagaactc agatggcttt
     1621 aatccttttc gctatataga aacagaaaac gatttgaatc gcatgctgac tgtttatttt
     1681 aataacacca aaggctctgg ctcccgaagt gatccatttt gggatgaagc ttctatgact
     1741 ttggttcgag ctttagcctc ttacttagtc gatttctata atccacctaa aacaagagaa
     1801 cagctcatag aagaaagtcg tttaagtcaa aaggaatatc aaaacttgtt gaaacgtcaa
     1861 aaaaaagaag tggaagagcg aaaaaaacga ggtcgttatc caagttttgc tgaaatctca
     1921 aaactcatta aacacttatc caagggtgaa aaccaggaaa aaagtgtctt agaaattcta
     1981 tttgaaaatt atgctaaaaa gtatgggact gaaaatttta ccatgcgaaa ttgggcagat
     2041 tttcaaaatt ataaggataa gactctggat tctgttatag ctgtaaccac tgctaaattt
     2101 gccctcttca atattcaaag tgtcatggac ttgaccaaaa gagataccct tgatatgaag
     2161 acatggggca aggaaaaatc aatggtttac ttagttatcc cagataacga tagtaccttt
     2221 cgctttcttt cagccctctt tttttcaacc gtatttcaaa ccctaacaag acaagcagat
     2281 attgatttta agggtcaatt gcctcttcat gtgagagttt acttagatga gttcgcaaat
     2341 atcggagaaa ttccagattt tgctgaacaa acctcaacag tccgttctcg aaatatgagt
     2401 ctcgttccta ttcttcaaaa tattgcccaa cttcaagggc tctataaaga aaaagaagct
     2461 tggaaaacca ttttggggaa ctgtgatagc ttagtctact taggtggtaa tgatgaagat
     2521 acctttaaat ttatgagtgg cttactcggt aaacaaacca ttgatgttcg aaatactagt
     2581 cgttcctttg gccaaacagg ttcaggatcc ctttctcatc aaaagattgc tcgtgactta
     2641 atgacacccg atgaggtcgg aaatatgaaa cggcatgaat gcttggttcg aattgccaat
     2701 atgcctgtct ttaaaagcaa aaaatacaat tccactaagc atccaaactg gaagtaccta
     2761 gctaatcaag aaagcgatga acggtggtgg aactatcaaa tcaatccttt gaatcaaaga
     2821 caagaaaatt atcttgacga ccttagaatt cgtgatttaa cttttgaatc tagtttaaaa
     2881 taaaaataga aaagaggaaa tagatgatta cgcattttaa aggttttgtt tatggagtag
     2941 acgcaagtgc tatgtttgca caagctatgt ctttgttaca gaagggattg attgcagttg
     3001 gtgcctttct cgttgttgtg gggattgtca accttgcaac caacattaaa gatggtggac
     3061 caggtgttcg gaatgccatt cttgaaattg tcggtggtgt tatggtaggg gctgctggag
     3121 cctttgtaac ccagatttca atttaggagg ataaacaatg acatgaatct tagtttagtc
     3181 tcaccctttg tttaccttgc atctgaaaaa atatcagctg aaaatttatt tgaaggattt
     3241 aatgtggatt tacaatctac ggtagatctg attaaatccc tatctagcta caatccaaca
     3301 gtttggactt atatgtctag tattactaaa agtgtcatgc agcctcttgg agttgcgatt
     3361 ttatcagttg ttctcatctt agaattttcg aagatggcaa agaagattgc taactctggt
     3421 ggagcgatga cctttgaagc attagcgccg atgttgatta gttatattat ggtcgcagtg
     3481 gtcattacca atactaccgt tatagtagaa gctatcatcg ggattgcgag tcacgccatc
     3541 gaacaagtgg cctcgattgt ggctcacggt ggggcgaagt atgatacact ctctggatta
     3601 aaaggttcag gatttattgg ccggatgatt gtgggctttt tcgctctcct tatctggctt
     3661 gttcggatag taagtgcagc catggttaat cttttggtat ctattcgatt tattcaactc
     3721 taccttatga tcccatttgc ccctcttacg attccaacat ttttaagtga tgaatggaag
     3781 tctattggta ttggctattt aaaaaatatt atggtctatg cggtacaagg ggttctcatt
     3841 tttctgattg tttctcttgt tcctttgttt gaatctgctg ggaaaatagc tgtttcaaat
     3901 ggtgcaggag tcttgcaatc acttgcgatt atgtttggta gtttagtaca agctatctta
     3961 ctgattattg ccctcgttgg ttctcaacgt acggctcgct caatcttagg tatgtaatta
     4021 gataaaggct aggaagtgat tgcttcttag cctttttaga aaggaaagtc atgaatacat
     4081 gtgtctttaa agacatctca aaataccaac acagggcttg gttaggtttc accacaagac
     4141 aaatcatctt tgttttacca gcctttattg tcacaattat tgttttgggc ttgaatctct
     4201 ttttctggca atttggagat tggtttgttt acggttttgt gtttgctttt accatccccc
     4261 taatgctttt tggagtctat aaacccaatg atttatattt tgaacattat ttgaaatacc
     4321 gtcttcattt tgaattaacg gttcccctac gcacaattac aggaaagaaa ggacctgaac
     4381 atgaaaagaa aatcaaatac attaaagaaa caaaaaactt caatgactaa taaaaaggaa
     4441 gaagttaaag ggaaaaaaga ggaagtgtta ccctcaacgg ctaatactct ttcctatcaa
     4501 gccctgtatc aaaatggtct gatgcaggta aaagaagatt atttctcaca aagctattta
     4561 cttggtgatg tcaattacca gaccgttggt ttagaagata agggcgcaat cattgagaag
     4621 tattctgatt tgattaactc actagatgac caaaccaact tccaattgac tatctttaat
     4681 aaaagattga atttagaaaa gtttagacag agtgttctgt atgaggaaaa agaagatggg
     4741 tatgatagct atcgtaaaga attgaatcgg atgatgaatc agaatttaga cagtggtgaa
     4801 aataattttt cagctgtgaa actgattagt tttggtagaa aggattctaa tcccaaacaa
     4861 gcctatcgtt ccttgtctca aataggcgaa tatttcaaga gtggtttctc agaaattgat
     4921 gcacgatttg aatccttggc tggagaagaa cgggtgaact tgttggccga tatgcttaga
     4981 ggagaacacc atcttccttt ttcttaccgt gatttaacga gatcgggtca gacaactcgt
     5041 cacttcatag cacctaatct cttggatttt aaaaacaaga attacctaca aatcaatgac
     5101 cgtttattac agattgtcta tgtgagagac tacggtatgg aattaggaga tcagtttatc
     5161 agagacctca tgcaaggaga tttggaattg attgtaagcc tccatgctca aagttcgacc
     5221 aaggcagatg ccatgaagaa actacgaaca aagaagacct tgatggaatc ccaaaagatc
     5281 ggggaacaac aaaaacttgc tcgtacaggt atctatttgg aaaaagtagg tcatgtatta
     5341 gaaagcaata tcgatgaagc tgaggaactc ttaaaaacca tgaccgagac aggagataaa
     5401 ctatttcaaa cagtcttctt gattggtgtt tttggtcagg atgaagaaga actcaaacaa
     5461 gcactagaca ctattcaaca agtggctggc tcaaatgacc taatgattga taaactacca
     5521 tatatgcaag aagcagcctt taatagtttg ctaccatttg gttgtgattt tttagaggga
     5581 gtatcacgga gtttattaac atctaatgta gcagtgaact caccttggac ttcagtagac
     5641 ttacaagacc gtagtgggaa atattacggt atcaatcaaa tatcaagtaa tattattacc
     5701 attgatcgca gcctattaaa tacaccgtct ggtctgattt taggaacatc tggagctggg
     5761 aaagggatgg caaccaagca tgaaattatc acgaccaaaa tcaaggaatc tggtgaaaat
     5821 actgaaatta tcatcgtgga tccagaagca gagtacagtg tcattggacg aacttttggg
     5881 ggagaaatga ttgatattgc gcctgattcc caaacctatc tcaatgtcct tgacttgtct
     5941 gaggaaaata tggatgagga tcctgtaaag gtaaaatcag aatttctttt atcctttatc
     6001 ggtaagttat tggatagaaa aatggatggg agagaaaaat cgattatcga ccgagttacg
     6061 agactcactt atcagtcatt taaagagcct tctttggaag aatgggtctt tgtcttgagc
     6121 caacaagcag aagaagaagc gcagaatttg gcacttgata tggaactgta tgttgaaggt
     6181 tctcttgata ttttttctca taagaccaat attcaaacag ggtccaattt cttaatctat
     6241 aatgttaaaa agttaggaga tgagctgaaa caaattgccc ttatggttgt ttttgatcag
     6301 atatggaatc gtgtcgttcg gaatcaaaaa ttagggaaga agacctggat ttatttcgat
     6361 gagatgcagc ttctcttatt agataaatat gccagtgatt tcttctttaa attgtggagt
     6421 cgtgtcagaa aatatggagc cagtccgact gggataaccc aaaacgtcga aaccttattg
     6481 ttagatccaa atggtagacg gattattgca aatagtgaat ttatgattct cctcaagcaa
     6541 gcaaaaaatg atagagaaga attggttcaa ctcttaggct tgtcaaaaga actcgaaaaa
     6601 tacctagtca atccagaaaa aggggctgga ctaataaaag ctggttcagt tgtcgttccc
     6661 tttaaaaata agattcctca aggtactcaa ttgtttgata tcatgagtac ggatcctgat
     6721 aaaatggctt ctaattaagg ggaaggtaaa tgaaggataa aagagaaatc atacgtgccc
     6781 gaaaggcatt tagaagaagt ctaaaagatg agaagaaatt cttgaaaaaa ggaaagaagg
     6841 aggtaaggaa acagaaaaaa gattccgctg tactggatga aaaagcatgg aaaaaagaga
     6901 taaaagaaaa gctagaggag atgagagaag cttcaaaggc tagagtaaaa caagcaaatg
     6961 aagactacaa tcacatcctt caaaatactc ctccatctct tttgaatcgg aaagaattaa
     7021 gagacagacg attgcctcat gctaggaaac gattgaaaat agccaagaag caatttagag
     7081 aagccaaggt agaagcaaaa gaagaaagaa aagagagtcg taaagaaaga aaaaccaatc
     7141 aaaaatttct ttatggtcag gaatcgaaag caaaatccaa ttttttcttt caagggaaga
     7201 gtttagaaga attaaaagct aagaaagaag tcaaggccgc aaaagataat ctaaaatcta
     7261 ctaaacaagc ctataagtcc aaaaaagtca gtaggaaagc caaaactttt ctttatgtcc
     7321 ttggacgtga aggtggagag ttagcttcag aaaatgaaga tttagaaggc tatcgtacac
     7381 ttcaagagac cattagaaaa gggaaacgct acagtcgact ttcttataat cttggaaaag
     7441 ctagtgtcaa aacaggacaa gcaacaggtc gttttaccaa gaaaagactg accaacacaa
     7501 aagagcgata ccatcatttt aaggatggaa aaggatggaa actagcgaaa gataatccaa
     7561 gttcctttaa aaatcggtat cgaaaattaa agaaacaagg tctttcaagt gtccgaaata
     7621 tctatcaaaa actaaaagca gccttttcct tctttacatt tacggctggg aatcctgcaa
     7681 cctggatagt tggaggatta gtctttcttc tcttacttat gatgagcttc tttttaggat
     7741 tttcatctgc tagtttgatt caacaagatg aatttgaatt aacaaaagct tatacccatc
     7801 taacttggga agatgcagaa catactcgca caaatgacaa aggaattact tattacacaa
     7861 aagttgatga tgtgatgggc tatatgaact ttaaattcca tgactatgag ttacacaaac
     7921 cagttcactt atttagttca gaaacttaca aggattatct gtctactttg tggcatgatt
     7981 taaacgatgg ggatgatttg aaatccatgc aagacctcta tgaaactcct aagtataaac
     8041 tctcgaaaga cgatcaagag gaaatgaagg aactaaaaga agagggtgta tatgcttcca
     8101 tgcaggaatt ggacaatcca tttgagggga aaagcaacga agatagtcta accatgactt
     8161 atcgttatgg atactatgat ttagatggga aacctactct tcaggagtac attctactag
     8221 aagcaaaggc tcaccaaacg attgtcgcac caatggatgg agttgtatct ctagatggtg
     8281 acaatattat tctcactaac ggaaaaggag agaatgagag tcgattgacc ttgtattcta
     8341 ttcataatgg ccgtgcgatt gaggggacaa gagtcttaac gggtgatatt attggtgaaa
     8401 caccagacga tacaggtttg aaagtttcct atcaaaagta taagaacaag aaagaaaaat
     8461 tggtgtatgt caatccgcaa ttttattttc caaaagtcat tcaacttcag acaactatct
     8521 tacctgccat tggtcagttt ggtggggatg agtttgaacg agcaaaacat atttatgagt
     8581 ttttgaaatc tcaaggggca agtccccaag ccattgcggc tattttagga aattggtcgg
     8641 tagaatcttc tattaatcca aaacgagctg aaggggatta tttatctcct cctgttggcg
     8701 ctaccgattc ctcatgggat gatgaaacct ggttagcaat tggaggtcca gccatttata
     8761 gtggtgctta tcctaatatc cttcatagag gtttggggtt aggtcaatgg acggatactg
     8821 cagatggttc aacacggcat acagctttat tgaattatgc acatatcaaa aataagaagt
     8881 ggtatgattt agatctccaa cttgatttta tgcttcatgg ggatagtcct tactatcaaa
     8941 gttggttaaa ggatttcttt ggaaatacgg gcagtgcagc caatttagcc caactcttcc
     9001 ttacctattg ggagggaaat tctggtgaca aactactgga aagacaaacc agagcaacag
     9061 aatggtatta ccaaattgaa aaaggcttta gccaaacaaa cggaggacag gcaaagagtg
     9121 atccacaatc cctcgaaggt gttcgtgggg acttgtatga tcattctgtt cctggtggtg
     9181 gagatggtat ggcctatgcc tatggacaat gtacatgggg tgttgcggct cgtatgaatc
     9241 agttaggctt aaaattaaaa ggaagtaatg gtgagaagat ttcaatcatt aataccatgg
     9301 gaaatggtca agactgggtt gcgacagctt caagtcttgg tggggaaact ggctctacac
     9361 caagagcagg tgccattgtt tcttttgtag gaggtacaca tggcacacca gctatctacg
     9421 gtcatgtggc ttttgtcgag aaagtctatg atgatggttc tttccttgtg tctgaaacta
     9481 actatggtgg caaccctaac tataccttta gaaaaatctc gcaagcagat agtgccatca
     9541 gttttgctta tacggtgaaa taaaagaatc atgtcatcag tttaggtgac atgattctta
     9601 ttttttatag agtgacatat ttaatgccat aggttgataa cttacttgat gatcgtcttt
     9661 aatcaattga ctgtaagcaa caaatccgaa ctgttcataa aattgaatga ctttaggatg
     9721 attgatacta tctaaaaata ccatctgagt tcctacaatt gctcgaaatt cttcaatttt
     9781 ttgaattaca atttcaaata actcttgtcc tgatagttta
//
LOCUS       DAVMGZ010000072         9688 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000072 DAVMGZ010000000
VERSION     DAVMGZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9688
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.073"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..344
                     /locus_tag="VYQ93_001384"
     CDS             <1..344
                     /locus_tag="VYQ93_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336057.1"
                     /translation="LDILLGTFLQISIVSIGWLVLYSGLFEAGVFLLANKGVAVKIKE
                     VDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHIFIVFNALMYLLSSS
                     QVSLKKNKLSS"
     gene            396..1085
                     /locus_tag="VYQ93_001385"
     CDS             396..1085
                     /locus_tag="VYQ93_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3336058.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            1127..1360
                     /locus_tag="VYQ93_001386"
     CDS             1127..1360
                     /locus_tag="VYQ93_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336059.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            1511..2122
                     /locus_tag="VYQ93_001387"
     CDS             1511..2122
                     /locus_tag="VYQ93_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3336060.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            2283..3077
                     /locus_tag="VYQ93_001388"
     CDS             2283..3077
                     /locus_tag="VYQ93_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV3336061.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3074..3709
                     /gene="trmB"
                     /locus_tag="VYQ93_001389"
     CDS             3074..3709
                     /gene="trmB"
                     /locus_tag="VYQ93_001389"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV3336062.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            3835..4314
                     /gene="rimP"
                     /locus_tag="VYQ93_001390"
     CDS             3835..4314
                     /gene="rimP"
                     /locus_tag="VYQ93_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV3336063.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4358..5494
                     /gene="nusA"
                     /locus_tag="VYQ93_001391"
     CDS             4358..5494
                     /gene="nusA"
                     /locus_tag="VYQ93_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV3336064.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5516..5809
                     /locus_tag="VYQ93_001392"
     CDS             5516..5809
                     /locus_tag="VYQ93_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV3336065.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            5802..6101
                     /locus_tag="VYQ93_001393"
     CDS             5802..6101
                     /locus_tag="VYQ93_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV3336066.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6118..8910
                     /gene="infB"
                     /locus_tag="VYQ93_001394"
     CDS             6118..8910
                     /gene="infB"
                     /locus_tag="VYQ93_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV3336067.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9161..9511
                     /gene="rbfA"
                     /locus_tag="VYQ93_001395"
     CDS             9161..9511
                     /gene="rbfA"
                     /locus_tag="VYQ93_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV3336068.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         2951 a         1801 c         2222 g         2714 t
ORIGIN      
        1 tgttggatat tcttttaggg acatttttac aaatctctat cgtatctatt gggtggcttg
       61 ttctttacag tggtttgttt gaagcaggag ttttccttct tgctaataaa ggggtggcgg
      121 taaaaatcaa ggaagtagat attcgaaatc gctttaaatt tatttttgga aaaaccttat
      181 ggtttcaaat tcttttgctc atctttttga taatcaaact ttatcttggt ttggatgcga
      241 ggttgatttt attctatgga catattttca ttgtctttaa tgccttaatg tatctattat
      301 ctagtagtca ggttagcctt aaaaaaaaca aactgtcttc ttaatcttac agcagtagag
      361 cctgacaagg tgaggtcata gtagggagga tttctatgaa aaagtatcaa cttctattca
      421 aaataagtgc agttttctct tacttatttt tcgtatttgg tctttctcag atgacgctta
      481 ttattcaaaa ttattggcaa ttttcttccc agattggcaa tttcgtctgg attcaaaatt
      541 tcttgagttt gctatttagc ggagtcatga tttggattct ggttaagaca ggtcatggtt
      601 atctctttca cattccaaga aaaaaatggc tttggtattc gattttgaca gtattagtgg
      661 tagtgctcca gatctctttt aacgttcaga cagctaaaca tgttcagtca actgctgaag
      721 gttgggctgt attgatcggt tatagtggga ccaactttgc tgagctaggt atctatataa
      781 ctttgttctt tctgactcca cttatggaag agctaatcta tagaggatta ctgcaacacg
      841 ccttttttaa gcattcgaga tttggccttg atttgcttct tccgtcaatt ttatttgctc
      901 ttcctcattt ttcaagcctg cctagtttgt tagatatctt catctttgca acatctggca
      961 tcatctttgc tagtttgacc cgctatacca agagcattta tccttcctat gcggtgcatg
     1021 tgatcaataa tattttcgca acattaccat ttttgctgac ttttttacat agggtcttgg
     1081 ggtaaaaaaa caaaaggctt gcttttcagc catagaggag attatcatgt ataaacactt
     1141 atttttccta gattccaaaa ctttagatcg gttgacaccc tatattctag tcttggcttc
     1201 tgacaccatt gcttttaatg tttttgtgct aacctttgta tctgcggtgg tttttaattt
     1261 cctaaattcc atgctagctt taatggctat attcataggg gctggctatg tggtcggatt
     1321 ttggttacta atactcaatg aaaatcaaag agcaaactag gaaactagcc gcaggctgta
     1381 cttgagtacg gcaaggcgac gttgacgtgg tttgaatttg attttcgaag agtataaaat
     1441 ggtttgtttt ggaaaggcta gaactaaaga atgacgcgta ggaagtctgt ttggtgagga
     1501 ggatagtttt atggagtttt ttgataaatt tcatgccttg tgttttggat ttttagtact
     1561 aataattgtc attacagttc cttatacgat taaccatggg ggtttttttc aaaatgaatc
     1621 tgcattgatt cttgtaagtc ttcttgtaac ctcgctgagt gttgcttatg ctagaaagtt
     1681 tgaaatgatt tcttttggga tgttaagcaa gaaacaactt ttgcttttca ttgcaatctt
     1741 tcttctaagt gtacttgaga cgctggttta tattcatttc ttcgctgttt cttctggctc
     1801 aggggtccaa cacttggcgg aagtcagcag aggaatttcc ctgtctttga ttttgactac
     1861 ctcagttttt ggccccatcc aggaggaact cattttcaga ggacttcttc aaggtgcggt
     1921 ttttgacaat tcttggttag ggcttgtgct aacttcctct ctcttttctt tcatgcatgg
     1981 accttctaat gtcccttcgt ttatttttta tctacttggg ggtttgttgc tgggctttgc
     2041 ttataaaaag agtcaaaacc tatgggtttc tactctagtc cacatgtttt acaacagttg
     2101 gccactccta tattatttat aaaaattatg aaaaggtaag cagaagatgg tgcttacctt
     2161 tttcaagcta taatgatacc caagccaatc cagaggcttt tctttgagaa tcgggttaga
     2221 gtggttgacg aataggccaa aaactagtag aatagtaagg aaactttata cggaggaaag
     2281 aaatggattt gggtgataat gagctaacac tgactcccat acctgggaaa agtggcaagg
     2341 cttatatggg tagctatcct gacgggaagc gcatctttgt aaaaatgaac acctctccaa
     2401 tcctacctgg tctagctaga gaacaaattg ctccacaatt attatggagt cgtcgtttgg
     2461 cagatgggcg tgatatgtgt gctcaagaat ggttgacagg caagatattg accccctatg
     2521 atatgaatcg taagcaaatc gtcaatattt taacccgtct tcatcgctca cgtccgttga
     2581 tgacacaatt gagtcgtttg ggctatgcca tggaaacacc tgtagattta ctacagtctt
     2641 ggcaggaaac ggctccagat gctttgcgta aaaatcattt tatcagtgaa gtgatggctg
     2701 atttacgtca gactattcca ggatttagag aggaccatgc gaccattgtc catggagatg
     2761 tacgacatag taattggatt gagacagata gtggcttgat ttatttagta gattgggatt
     2821 cggttcgctt gaccgatcgc atgtttgatg tggcccatat gctctgccat tatatttcag
     2881 aacatcagtg gaaggaatgg ttgacctact acggttacaa gtacaatcaa acggtattaa
     2941 gtaaattgta ttggtatggt caattgtctt atttgagtca gatttccaag tattatatga
     3001 accaagattt agaaaatgtc aatcgggaga ttcatggttt gcgtcatttc cgagacaagt
     3061 atggaaagag aagatgagag ttagaaatcg taaaggggca acagaattac tagaggcaaa
     3121 tccccagtat gtggtcctca atcccttgga agccaaggca aaatggcggg acttgtttgg
     3181 caatgataat cccattcatg tggaagttgg aagtggaaag ggtgcctttg tttcaggtat
     3241 ggccaagcaa aaccctgaca tcaactatat cgggattgat attcaaaagt ctgttttgag
     3301 ctacgctttg gacaaggtgc ttgaagttgg agtgcctaac atcaagctct tgtgggtaga
     3361 tggttctgac ttaactgact attttgaaga cggtgagatt gatcgcttgt atctgaactt
     3421 ttcagatcca tggccgaaaa aacgccatga aaagcgtcgt ttgacctaca agaccttctt
     3481 ggataccttc aaacgtatct tgcctgaaaa tggagaaatt catttcaaga cggataaccg
     3541 tggcttgttt gagtacagtt tagtgagctt ttctcaatat ggcatgaaac tcaatggtgt
     3601 ctggttagat ttgcatgcca gtgattttga aggtaatgtc atgacagaat acgagcaaaa
     3661 attctcaaac aaggggcaag ttatctaccg agttgaggca gaattttaag agatagccta
     3721 aaattaggct gtacaagtgc ttttgcttta cataagttgg caaacgtgct atactgatag
     3781 taagaatatg aaaagtgagg cggggaaata tcttcgcctc ttgcttatga ggaggtggac
     3841 gcaatcgcaa caatcgtaga attagtcaga gaagttgtag aacctgtcat agaagctcct
     3901 tttgaactcg tggatatcga gtatggaaag attggcagtg acatgattct cagtattttt
     3961 gtagataaac ccgaaggaat taccttgaac gacacggcag acttgacaga aattatcagt
     4021 cctgtcctag acaccatcaa gccagatccc ttcccagaac aatatttcct agaaattacc
     4081 agtccaggtt tggaacgccc tttgaaaacc aaggatgccg tcgctggagc ggttggaaaa
     4141 tacatccatg tcgggctcta ccaagccatc gataagcaaa aggtctttga aggaaccttg
     4201 ttggccttcg aagaggacga gttgactatg gaatatatgg acaagacgcg taagaaaacc
     4261 gtccaaattc catacagttt agtatcaaaa gcacgtttag cagttaaatt atagaaaaag
     4321 aaaggatagc ttttgaggat tcaaaagtga agaaaacatg agtaaagaaa tgctagaggc
     4381 cttccgcatt ttggaagaag acaagggaat caaaaaagaa gatatcatcg acgcagtagt
     4441 agagtcgctt cgttccgctt atcgcagacg ctatggtcag tcagacagcg tagctattga
     4501 cttcaacgaa aaaacaggtg actttacagt ttatactgtc cgtgaagttg ttgatgaagt
     4561 atttgatagc cgtttggaaa tcagcttgaa agatgctctt gccattaatt cagcttatga
     4621 acttggagac aaaatcaagt ttgaagaagc accagctgag tttggtcgtg tagcagccca
     4681 gtctgccaaa caaaccatca tggaaaaaat gcgcaagcaa acacgtgcca tcacttacaa
     4741 tacttacaaa gaacatgagc aagaaatcat gtctggtaca gtagaacgct ttgacaaccg
     4801 ctttatctat gtcaaccttg gtagcatcga agcccaattg tcaaaacaag accaaattcc
     4861 tggagaagtt tttgcttctc atgatcgtat cgaagtttat gtttacaagg ttgaagacaa
     4921 ccctcgtggt gtgaatgtct ttgttagccg tagtcatcca gaaatgatca aacgtttaat
     4981 ggagcaagaa attccagaag tttatgatgg aactgttgaa atcatgagcg tggctcgtga
     5041 agcaggtgac cgtacgaagg ttgctgttcg tagccacaat ccaaacgtgg atgctatcgg
     5101 tacaatcgtt ggacgtggtg gtgctaatat caagaagatt actagcaaat tccacccagc
     5161 tcgttacgat gctaaaaatg accgcatggt accaatcgaa gaaaatatcg atgttatcga
     5221 gtgggtagca gatccagctg aatttatcta caatgccatc gctcctgctg aggttgacca
     5281 agttatcttt gatgaaaacg acagcaaacg tgccttggtg gttgttccag ataacaagct
     5341 ttctcttgcc attggtcgtc gtggacaaaa cgtgcgcttg gcggctcact tgactggtta
     5401 ccgtatcgat atcaagtctg ctagcgaatt tgaagccatg gaagacgctg cttcagtaga
     5461 gttggaagta gaaaacgata ctgtagaaga ataaaagctg ctagaggagg gaaagatgaa
     5521 aacaagaaaa atccctttgc gcaagtctgt tgtgtctaac gaagtgattg ataagcgtga
     5581 tttgctccgc attgtcaaga acaaggaagg acaagtcttt attgatccta cgggcaaggc
     5641 caatggccgc ggcgcttata tcaaactaga caatgcagaa gccctagagg cgaaaaagaa
     5701 gaaggtcttt aaccgcagct ttagcatgga agtggaagaa agcttttatg acgagttgat
     5761 cgcttatgtg gatcacaaag tgaaaagaag agagttggga cttgaataag caaaagataa
     5821 gtaatctctt ggggcttgct cagcgagcag ggcgcatcat atcgggtgaa gaattggtgg
     5881 tcaaggccat tcaagacggc aaggccaagt tggtctttct agctcatgat gctggaccca
     5941 atctgaccaa gaagattcaa gataaaagtc attattatca agtagaaatt gtaaccgtgt
     6001 tttcaacact ggaattaagc atagcagtcg ggaaatcgag aaaggttttg gctgtaacag
     6061 atgctggatt tacaaagaaa atgaggtctc ttatggaata gaagaggagg acatgatttg
     6121 tctaagaaaa gattgtacga aatcgcaaaa gaacttggaa aagaaagtaa agaagttgta
     6181 gcgcgtgcaa aagagttggg cttggatgtg aaaagccact catcaagtgt ggaagaagct
     6241 gtcgctgcaa aaattgctgc cagctttaag cctgcagctg ctccgaaagt agaagcaaaa
     6301 cctgcagcac caaaagtaag tgcagaaaag aaagccgaaa aatctgagcc agctaaacca
     6361 gctgtagcta aggaagaggc aaaaccggct gagccagttg ctccgaaaac agaaaaagta
     6421 gcagtgaaac cgcaaagtcg taatttcaag gctgagcgtg aagcccgtgc taaagagcag
     6481 gcagaacgac gcaagcaaaa taagggcaat aaccgtgacc aacaacaaaa cggaaaccgt
     6541 cagaaaaacg acggccgtaa tggtggaaaa caaggtcaaa gcaaccgcga caatcgtcgc
     6601 tttaatgacc aagctaagaa gcagcaaggt cagcaaaaac gtagaaatga gcgccgtcag
     6661 caagaggata aacgttcaaa tcaagcggct ccacgtattg actttaaagc ccgtgcagca
     6721 gccctaaaag cagagcaaaa tgcagagtac gctcgttcaa gtgaggaacg cttcaagcag
     6781 tatcaggctg ctaaagaagc cttggctcaa gctaacaaac gcaaggaacc agaggaaatc
     6841 tttgaagaag cggctaagtt agctgaacaa gcacagcaag ttcaagcagt ggttgaagtc
     6901 gtccctgaga aaaaagaacc tgcagtggat acacgtcgta aaaaacaagc tcgaccagac
     6961 aaaaatcgtg acgattatga tcatgaagaa gatggtccta gaaaacaaca aaagaatcga
     7021 agtagtcaaa atcaagtgag aaatcaaaag aatagtaact ggaataacaa caaaaagaac
     7081 aaaaaaggca ataacaagaa caaccgtaat cagactccaa aacctgttac ggagcgtaaa
     7141 ttccatgaat tgccaacaga atttgaatat acagatggta tgaccgttgc ggaaatcgca
     7201 aaacgtatca aacgtgaacc agctgaaatt gttaagaaac ttttcatgat gggtgtcatg
     7261 gccacacaaa accaatcctt ggatggggaa acaattgaac tcctcatggt ggattacggt
     7321 atcgaagcca aacaaaaggt tgaagtggat aatgctgaca tcgaacgttt ctttgtcgaa
     7381 gatggttatc tcaatgaaga tgaattggtt gagcgtccac cagttgttac tatcatggga
     7441 cacgttgacc acggtaaaac aacccttttg gatactcttc gtaactcacg tgttgcgaca
     7501 ggtgaagcag gtggtattac tcagcatatc ggtgcctacc aaatcgtgga aaatggtaag
     7561 aagattacct tccttgatac accaggacac gcggccttta catcaatgcg tgcgcgtggt
     7621 gcttctgtta ccgatattac gatcttggtc gtagcggcag atgacggggt tatgcctcag
     7681 actattgaag ccatcaacca ctcaaaagca gctaacgttc caatcatcgt agccattaac
     7741 aagattgata aaccaggtgc taacccagaa cgcgttatcg gtgaattggc agagcatggt
     7801 gtgatgtcaa ccgcttgggg tggagattct gaatttgttg aaatctcagc taaattcaac
     7861 caaaatatcg aagaattgtt ggaaacagtc cttcttgtag ctgaaatcca agaactcaag
     7921 gcagacccaa cagtgcgtgc aatcggtacg gttatcgaag cgcgcttgga taaaggaaaa
     7981 ggtgcggtcg caacccttct tgtacaacaa ggtaccttga atgttcaaga tccaatcgtt
     8041 gtcggaaata ccttcggtcg tgtccgtgct atgaccaacg accttggtcg tcgtgttaaa
     8101 gttgctggac catcaacacc agtctctatc acaggtttga acgaagcacc gatggcgggt
     8161 gaccactttg ccgtttacga ggatgaaaaa tctgcgcgtg cagcaggtga agagcgtgcc
     8221 aaacgtgccc tcatgaaaca acgtcaagct acccaacgtg ttagccttga aaacctcttt
     8281 gataccctta aagctgggga actcaaatct gttaatgtta tcatcaaggc tgatgtacaa
     8341 ggttctgttg aagccctttc tgcctcactt caaaagattg acgtggaagg tgtcaaagtg
     8401 actatcgtcc actcagcggt cggtgctatc aacgaatcag acgtgaccct tgccgaagct
     8461 tcaaatgcct ttatcgttgg tttcaacgta cgccctacac cacaagctcg tcaacaagca
     8521 gaagctgacg atgtggaaat ccgtcttcac agcattatct acaaggttat cgaagagatg
     8581 gaagaagcta tgaaagggat gcttgatcca gaatttgaag aaaaagttat tggtgaagcg
     8641 gttatccgtg aaaccttcaa ggtgtctaaa gtgggaacta tcggtggatt tatggttatc
     8701 aacggtaagg ttgcccgtga ctctaaagtc cgtgttatcc gtgatggtgt cgttatctat
     8761 gatggcgaac tcgcaagctt gaaacactac aaagacgacg tgaaagaagt gacaaacggt
     8821 cgtgaaggtg gattgatgat cgacggctac aatgatatta agatggatga tgtgattgag
     8881 gcgtatgtca tggaagaaat caagagataa gattttttgc tcctttctta ggtggtgagg
     8941 gacgcaagca aaccgatggt ttcattgctt atttttgagc ctagggtctc aaaaatcccc
     9001 tgtgatggga ctgataaatc agttccatca ctttcaccac ggcgaaagaa gcagatgact
     9061 tcaaattgaa cttcgtttca atttaaactg aaaatcaaga agtttaaaat agctaggtct
     9121 gctggcctag cttttggttc aaagtagaga aaggaatatc atggcaaatc atttccgtac
     9181 agatcgtgtg ggcatggaaa tcaagcgtga agtcaatgag attttgcaaa agaaagtccg
     9241 tgatccacgt gtccaaggtg tgaccatcac agatgttcag atgctgggtg acttgtctgt
     9301 tgccaaggtt tattacacca ttttgagtaa ccttgcttcg gataaccaaa aagcccaaat
     9361 cgggcttgaa aaagcaactg gtaccatcaa acgtgaactt ggtcgcaatt tgaaattgta
     9421 caaaatccca gatttgacct tcgtcaaaga cgagtccatc gagtatggaa acaagattga
     9481 cgagatgcta cgcaatctgg ataagaacta aagaagaggg gttgcccctc ttttttggtg
     9541 gaggaaaata ggttgaattt gaaatggaaa aatattcttt taaaatatat tgaaactaga
     9601 atagtacgcc tctacttcta aaacattgtt agaaatcgat ttgactgtcc tgatcgattt
     9661 gtcctgttct tgtttcattt tactataa
//
LOCUS       DAVMGZ010000073         9667 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000073 DAVMGZ010000000
VERSION     DAVMGZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.074"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            190..285
                     /locus_tag="VYQ93_001396"
                     /pseudo
     CDS             190..285
                     /locus_tag="VYQ93_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(538..1158)
                     /locus_tag="VYQ93_001397"
     CDS             complement(538..1158)
                     /locus_tag="VYQ93_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV3336069.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1158..1640)
                     /locus_tag="VYQ93_001398"
     CDS             complement(1158..1640)
                     /locus_tag="VYQ93_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV3336070.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1640..2482)
                     /locus_tag="VYQ93_001399"
     CDS             complement(1640..2482)
                     /locus_tag="VYQ93_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV3336071.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2577..3365)
                     /locus_tag="VYQ93_001400"
     CDS             complement(2577..3365)
                     /locus_tag="VYQ93_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV3336072.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQALK
                     HLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDKAYKS
                     HITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTMSNMN
                     EGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLESSPKT
                     DLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3509..4888
                     /gene="glmU"
                     /locus_tag="VYQ93_001401"
     CDS             3509..4888
                     /gene="glmU"
                     /locus_tag="VYQ93_001401"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV3336073.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLTGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVILKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4898..5443
                     /locus_tag="VYQ93_001402"
     CDS             4898..5443
                     /locus_tag="VYQ93_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3336074.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5455..5769
                     /locus_tag="VYQ93_001403"
     CDS             5455..5769
                     /locus_tag="VYQ93_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV3336075.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5786..6478
                     /locus_tag="VYQ93_001404"
     CDS             5786..6478
                     /locus_tag="VYQ93_001404"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV3336076.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6555..7046
                     /gene="rocS"
                     /locus_tag="VYQ93_001405"
     CDS             6555..7046
                     /gene="rocS"
                     /locus_tag="VYQ93_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV3336077.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7144..7734
                     /locus_tag="VYQ93_001406"
     CDS             7144..7734
                     /locus_tag="VYQ93_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV3336078.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7743..9506
                     /locus_tag="VYQ93_001407"
     CDS             7743..9506
                     /locus_tag="VYQ93_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV3336079.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         2993 a         1684 c         2059 g         2931 t
ORIGIN      
        1 aagacttttt gaccagcctc atttttttaa cttgatactc agtgaaaagc aaagattaaa
       61 ctagaaagct agccgcaggc tgctcaaaga acagctttga ggttgcagat aaaacttgtg
      121 aggtaaccaa catatataat gtgaagctga cgtggtttga atagatttta gaagggtatg
      181 agtctggaag ttttaatgga taatgcaaga ttccatagaa tgggtaagct agagttctta
      241 tgtgaagagt ttgggcataa acttttacct tttcctccct actcatctta gtatagaaaa
      301 gtgaatctaa aatagtacat aactgcttct aaaacattct tataaattga tttaaattct
      361 caaatcatat tattcagttc ttatttcatt ttgctctaca atcctgttga gaagacacgt
      421 gttcatatca aaaaggtatt ggcaagttgc aatacctttt tacaaggttc ttttgtctta
      481 tttttgtttc aactgactat atctcctatg gttctagttc agaaggctag gctataatta
      541 tgattgataa gaagtatcat tccaagtatt gagagtgaat gtttcaaaat catgggtttc
      601 tataatggtc aggctggcat ttgctagacc gccatcttta cgaagaagtg gttctttata
      661 gcctaggaga gtacgaagac tggcagtaag attggcgccg tgtccgacaa ttagaatacg
      721 ctcagctgga ctatctttta atgatttgat aaattggatg gtccgctgag ttgtactata
      781 gagggattcg gctccgaaca ttcgagtgtc aaattgagca agatttgaac gaaaagcctg
      841 gatttgttgc gggtaaatag cttccagggt tgcaattttc aaaccttcta acttcccaag
      901 ttgccattca cggagattag gaacgatttc taaagaacag ggggtataga gttgactttg
      961 gataatctca gcagatttga ccgctcgagg taaatcactt gaataaatct gatcaaaagg
     1021 aatttccttg agatactgac caagtcgttt tagggtttca atggattcag gaagaagagg
     1081 agaatcacca ctagcacctt gaaaacgacc ttcttggttc cagagggtac gaccgtggcg
     1141 gacaaagtag agtttcatta cttgatgtcc tccaaaatat ctacaaagtc tgcctttaca
     1201 aagctagcca agtcttgtgg cgcgacgata atgctgtgtc cgacttcgcc tgcagagaca
     1261 atcatttgat ccaaatctag agcaatttta tcgataaaaa tgggataatt gtgtttctga
     1321 cgaattccga caggattatt ggctccatga atgtaaccag ttgttttttc taagtccttt
     1381 tgtggaatca tgctcacttt tttattgcca gaaattttag ctagtttctt ttcagacaag
     1441 tgctgagtga tagggacaat tccgataatc ggtccggtct tgtctcccaa aagcgccaag
     1501 gttttgaaaa tctgatctcg ttcataacct tgaggaagct ctccttctag ggcattgatt
     1561 tgaatcccct gatgagggat agctgcttta gataggattt gttccaccaa tgtttttttg
     1621 attttaactt tttttgccat tatttatatt tatcctccaa ttgactcatc caaataccaa
     1681 gccagattcc cagcgcaaag aagaaggcga tgatgacata accgacaagt gaaagtcctg
     1741 tgtattggat actttcagcg tttcctgcat ttggaattaa gatcaaaagg gtacttgata
     1801 ggacgatacc gatgatgaaa tgatagacgc gtgagtggta gttgtttaag gcataatcca
     1861 tcaattttga aaaaacgatg agagttgcac ctgcaccaat tccaatcgga aagaaggttc
     1921 ccaagaaatc aaaagtttta aaaccagtca acataggagc atatagtccc aaaatcaaaa
     1981 gtaaatttga tgggctgagg ccaggaacca agacgccaag ggccaatagt gcgcctgcta
     2041 ggacgaagtt aagaaagctg gcgcttaagg ttccaacgac aaaatttaag gcatagagtc
     2101 ctaatccaga aatgataaag gttgtccata accaagctaa atcaatcttg tctcggtcag
     2161 attctcgagt tgattctttg aggaggctag gaactgtacc gataatagct cccgcaaagc
     2221 tccataatac aaaaacctga taattttcaa gcaggtattc aatcgggtag gaaaataagc
     2281 cgattcccag aagcataccg atggcaactg gaataaagta caaaacattt tctttaaagt
     2341 ctttaaaggg atgggccaga aagccaatca ttcgttcata gattcctaag attgctgcta
     2401 gaacccctcc ggaaattccc ggtaggataa atccaagagc aattacaatc cctttaataa
     2461 cgcgtgctaa ccatgagagc atatatttcc tccaactatt tctatttcaa aaaatcctta
     2521 ctatattgta tcacaatcag acacaaaaag aaaaagcaaa tgataaacaa atgcttttaa
     2581 ggttttaaaa aaagttttcg aaaggtttct tctttatttt ttaagggaga gataacgttg
     2641 atatctaaat cgtggtcaaa gccggcaatt tttcctttag atgtgtattg gtgaatatca
     2701 taatctaaat cagttttagg actgctctcc aaaaatcctg agtctgagcc gtaggacgga
     2761 atccaaacag aggtaaactt gcctgtatca atactgtgtt cttccatgaa gtagacacca
     2821 acgtagatgc cgatgttttt agcacctagt gatgctagtt ttgctcgaaa gttttcgaca
     2881 ccttcgttca tattagacat ggttttgtct tccacgtcaa gccaatagta actagggctg
     2941 taaggagagg cagcattgta gaaaacttcg gcagcctttt ccatttcttg gacacttttt
     3001 ccagctacat aagcgtagac agcaactggg acattccgct tttgaagttc agtgatatga
     3061 ctcttatagg ccttgtctat tccattgata aatgaagcat cattttcttt tgttgtttga
     3121 gcaccactgt gaacacgaac aatagcacct gaaatatttt gtgagagggc atcgtagttg
     3181 atttcctcag gacgctgcca gccagagagg tcaataatcg gtttgtctaa gtgtttcaaa
     3241 gcctgtgcta tattggattt tgttttaaac gattggctgt cattaagtgg gcgattgatg
     3301 attaaaatga acatcataat cccaaaaaaa ctaaataaaa taagtggatg aatttgtttt
     3361 ctcatatctt ataagtctac cctaaaaatc aaaaaaaatc aaaaaaatgg gttaaggaag
     3421 agactttaga gcattttttc attcaagagt gcggaatgat ttgaaatatg gtataataaa
     3481 agggaatttc tacagaaaag agaagattat gtcaaatttt gccattattt tagcagcggg
     3541 taaagggact cgcatgaaat ctgatttgcc aaaagttttg cacaaggttg cgggtatttc
     3601 tatgttggaa catgttttcc gtagtgtggg agctatccaa cctgaaaaga cagtaacagt
     3661 tgtaggacac aaggcagaat tggttgagga ggtcttgact ggacagacag aatttgtgac
     3721 tcaatctgaa cagttgggaa ctggtcatgc agttatgatg acagaaccta tcttagaagg
     3781 tttgtcagga cacaccttgg tcattgcagg agatactcct ttaatcactg gtgaaagctt
     3841 gaaaaacttg attgatttcc atatcaatca taaaaatgtg gccactatct tgactgctga
     3901 aacggataat ccttttggct atggacgaat tgttcgtaat gacaatgctg aggttcttcg
     3961 tattgttgag cagaaggatg ctacagattt tgaaaagcaa atcaaggaaa tcaacactgg
     4021 aacatacgtc tttgacaacg agcgtttgtt tgaggctttg aaaaatatca ataccaataa
     4081 cgctcaaggc gaatactata ttacagacgt cattggtatt ttccgtgaaa ctggtgaaaa
     4141 agttggcgct tatactttga aagattttga tgaaagtctt ggggtaaatg accgtgtggc
     4201 gcttgcgaca gctgagtcag ttatgcgtcg tcgcatcaat cataaacaca tggtcaacgg
     4261 tgttagcttt gtcaatccag aagcaactta tatcgatatt gatgttgaga ttgctccgga
     4321 agttcaaatc gaagccaatg ttatcttgaa agggcaaacg aaaattggtg ctgagactgt
     4381 tttgacaaac ggtacttatg tagtggacag cactatcgga gcaggagcgg tcattaccaa
     4441 ttctatgatt gaggaaagta gtgttgcaga cggtgtgaca gtcggtcctt atgctcacat
     4501 tcgtccaaat tcaagtctgg gtgcccaagt tcatattggt aactttgttg aggtgaaagg
     4561 atcttcaatc ggtgagaata ccaaggctgg tcatttgact tatatcggaa actgtgaagt
     4621 gggaagcaac gttaatttcg gtgctggaac tattacagtc aactatgacg gcaaaaacaa
     4681 atacaagaca gtcattggag acaatgtctt tgttggttca aattcaacca ttattgcacc
     4741 agtagaactt ggtgacaatt ccctcgttgg tgctggttca actattacta aagacgtgcc
     4801 agcagatgct attgctattg gtcgcggtcg tcagatcaat aaagacgaat atgcaacacg
     4861 tcttcctcat catcctaaga accagtagga gcctatcatg gagtttgaag aaaaaacgct
     4921 tagccgaaaa gaaatctatc aaggaccaat atttaaactg gtccaagatc aggttgaatt
     4981 accagaaggc aagggaactg cccaacggga tttgattttc cacaatgggg ctgtctgtgt
     5041 tttagcagta acggatgaac aaaaacttat cttggtcaag cagtaccgca aagctatcga
     5101 ggcagtctct tacgaaattc cagccggaaa attggaggta ggagaaaaca cagcccctgt
     5161 cgcagctgcc cttcgtgaat tagaggaaga aacagcctat acagggaaat tagaactctt
     5221 gtacgatttt tattcagcta ttggcttttg taatgagaag ttaaaactat atttagcaag
     5281 cgatttgaca aaagtggaaa atccgcgtcc gcaggatgag gatgaaacct tggaagtcct
     5341 tgaagtgagc ttagaagaag cgaaagaatt aatccaatca ggtcatatct gtgatgccaa
     5401 gacaattatg gctgttcagt attgggagtt gcagaaaaaa tagaggaggt cagtatgggt
     5461 aaatctttat taacggatga aatgattgaa agagctaata gaggcgaaaa aatttcaggt
     5521 cctcctttgc tagatgataa tgaggagact aagattttac caacctcttc ttcccgtttt
     5581 ggttatgcca atcctaagga tcatggtttt agccaggaaa ccttgaaaat tcaggtcgaa
     5641 ccatctattc ataaaagccg tcgcattgaa aataccaaga gaaatgtctt caattctaag
     5701 ttgaataaaa tcttattcgc ggtcatcttt ctcttgattt tgcttgtttt agcaatgaaa
     5761 cttttgtaat agaaaaggaa ttgaaatgaa aataggaatt attgctgcta tgccagaaga
     5821 actggcttat ctggtccagc atttagataa tacccaggag caagttgttt tggggaatac
     5881 ctatcataca ggaaccattg cttctcatga agtcgttctt gtagaaagtg gaattggtaa
     5941 ggtcatgtct gctatgagtg tggcgatttt ggctgatcat ttccaggtgg atgcccttat
     6001 taatacgggt tcagctgggg cagtagcaga aggtatcgct gttggggatg tcgtgattgc
     6061 tgacaaatta gcctatcatg acgtggatgt cacagctttt ggctatgctt atggacaaat
     6121 ggcgcaacaa ccgctttatt tcgaatcaga caaaaccttt gttgctcaaa tccaagagag
     6181 tttatctcaa ttggaccaaa actggcatct tggtttgatt gctacaggag atagttttgt
     6241 tgcaggaaat gacaagatag aagcgattaa gtcccatttc ccagaagttt tagccgtgga
     6301 gatggagggg gcagctattg ctcaagcagc gcatgccctc aatctcccag tcttagtcat
     6361 cagagctatg agtgacaatg ccaaccatga agcaaacatc ttttttgatg agtttattat
     6421 cgaagctgga cgtcgctctg cccaagtctt gttgaccttt ttgaaggctt tagattaagc
     6481 ggaaatttga cagtttttct agcttatgat aagatttaag taaagaaaag ctagaaaacg
     6541 tttcagagga tattatgagt attgaaatga ccgtcagtga gattgcagag gtcttaggat
     6601 tatctcgcca agcaatcaat aaccgtgtca aagaattacc agaagaagac acagataaaa
     6661 atgacaaagg ggtaacagtt gttaccagaa gtggcttgat taagctagaa gaaatctata
     6721 aaaaaacgat ttttgaagat gagcctgtca gtgaagatgt caaacaacgt gaactgatgg
     6781 agattctagt ggatgagaag aatgcagaaa ttcttcgtct ttatgaacaa ttaaaagcca
     6841 aggatcgtca gttatcagaa aaagacgagc agatgcgtat caaagaccgt cagattgctg
     6901 aaaaagacaa acaattagat cagcaacaac aattgaccct tcaagccatg aaggatcaag
     6961 aaaatcttaa gctagagctg gaccaagcaa aagaagaagt ccaatccact aagaaaggct
     7021 tttttgctcg tttatttgga ggataatcaa ggagctgttt aggttaaatg ctaacctgat
     7081 ggcttctttt gtttgtgaat agtatagttg attagaacag agacagtaga ggagaggtac
     7141 agaatggaac gattagaaga ttacattgct tttgacttag aatttaatca gcacgaagga
     7201 caaacacact tgattcaagt atcggcagtg cgttttagag atggtcggga aatcgatgcc
     7261 tatgattcct atgttcatac cagcgtccct ctaaaaagct ttatcaatgg tttgaccggg
     7321 attacagctg agaccttaaa agatgctcct ccagttgaga aagtcttaga agaattccaa
     7381 gagtttgtgg gttctttgct gatgataggc tacaatgctg ctaaaagtga tttacctatt
     7441 ttagcagagc atggtttaga ctatagccaa cagtacaagg tggatttgta tgatgaagct
     7501 tttgaacgtc ggagttcaga tttacatggt attgccaatc tcaaattgca aactgttgcc
     7561 tcatttcttg gatttcaagg tcagtctcat aatagtttag aggatgctcg gatgacggcg
     7621 cgtgtttacg aatccttctt agagtcggat caagctaggg aattgttaga gacagaaagt
     7681 agcttatcaa acaatccttt tggtggcttg gacttgtctc aattatttga ctaggagtgc
     7741 ctatgaaacc tgaaaaacct aaaaaattgg gtcttaaaca gatttacctc aatcttgata
     7801 aaatcctctt tctatttttc ttgattttta tgatagtgga gtatttgtgg gtaccattca
     7861 attcatggtt ggctagcttc ttattagatc agacaggtta tctttttatt tcctataaca
     7921 atgtcttagc catccttact cattctccat tgattagtct agctttttta gccttgattg
     7981 taattaacct tctggttgct tattttcaaa taggactcct ctttattggt gctcgtcatc
     8041 tcctctataa tgaaaagcga aggctaattg agtatatccg caaggttttc catgaaagtt
     8101 ttggatttat gaaaaagcta acttgcgcta aagccttgtt tatctttttc tatatagcta
     8161 tgctttttcc ttttatacgg aaaatgctga agatttatta tcttaataaa atcgtcattc
     8221 cagagtttat ccaagcttat ttagaagata aatattggat gtggtggctg agcatcctac
     8281 ttctatctct tatctttctc tatgtctctg tcagattgat gtttgccctt ccgaagattg
     8341 tatatgatca gctgactgtt cgagaagcag tgatgtttag cttggagaaa accaagaaga
     8401 gagttacttt ttatgcttgg aatttatttt acatcttgct caaagcacat ttattatttt
     8461 atcttccttt gattccttta ttattggctc aaactttggt agatgatatc actcagagag
     8521 agtctctgat tcttggtatt ttgaattttg tcgtgattaa aaatctctat tatatggctc
     8581 tgacttattt tttggttaaa tttgtttcat ttttgacagg aaaggagctg gatatgcttc
     8641 ctaggagaga aaaagatcac atcgtgcgat ggggtgtcat gacttgtgcc agtcttttct
     8701 ttgccttaga aggttatatt tatctggagg ctcccatggt tcatctacct caacttattt
     8761 ctcaccgagg ggtttccaat gcaaatggga ttcaaaatac agtagagtcc ttggaaacta
     8821 cagcacaact caaaccagac ttggtggaga cggacgtgca ggaaacaaaa gatgggcagt
     8881 ttgtcatgat gcatgatgct aacttgaaaa atctagcagg tatcaataaa agtcctcaag
     8941 acttaaactt ggaggagctt aaagggattg atatttttga aaatggctac cagactaaaa
     9001 tttcaagctt tgaagattat ctcagtcgag ccaacgaact tggtcaaaaa ttactaattg
     9061 aaattaaaac cagtaaaaaa gatagtccag acatgatgaa ccgcttttta gcccgttatg
     9121 ctgcaaagct caagatttat ggacatcaaa tccagtcttt agactaccat gttgtcgaaa
     9181 aagtaagaca gtatgatgcc gaactgccag tttatttcat catgccctac aattctgtct
     9241 ttcctaaaac aagagcgaca ggatatacta tggagtactc aaccttggat gaatattttg
     9301 taagcaaact atggacaacg gatcagaaac tttatgtctg gactgtcaat gattcagaag
     9361 ctatcagcaa atctcttcac ttaggagtag atggagtgat tacagatgat ttggaaaaag
     9421 ttcagcggga aatagaagta gcccaagaag atccagaata tacggatttg ctcttgaaaa
     9481 aagcattgga attctttgaa ttttagtaat aaaaaagagg aacggttgtt cctcccatga
     9541 taggtaagta ttcttgtgaa tgcttgcttt tttgtggttg caatccgttt acgagaattg
     9601 tttgtattgt tcttgtttca atttacttta tagtgctttg aagttggaat gctacactat
     9661 aacttct
//
LOCUS       DAVMGZ010000074         9667 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000074 DAVMGZ010000000
VERSION     DAVMGZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.075"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            159..5138
                     /locus_tag="VYQ93_001408"
     CDS             159..5138
                     /locus_tag="VYQ93_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV3336080.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTVQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTSDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFSDVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLHYQGHEVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            5228..6424
                     /locus_tag="VYQ93_001409"
     CDS             5228..6424
                     /locus_tag="VYQ93_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV3336081.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            6560..7087
                     /locus_tag="VYQ93_001410"
     CDS             6560..7087
                     /locus_tag="VYQ93_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV3336082.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7164..7520
                     /gene="glnR"
                     /locus_tag="VYQ93_001411"
     CDS             7164..7520
                     /gene="glnR"
                     /locus_tag="VYQ93_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV3336083.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7557..8903
                     /gene="glnA"
                     /locus_tag="VYQ93_001412"
     CDS             7557..8903
                     /gene="glnA"
                     /locus_tag="VYQ93_001412"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV3336084.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(8999..9118)
                     /locus_tag="VYQ93_001413"
     CDS             complement(8999..9118)
                     /locus_tag="VYQ93_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV3336085.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            9376..9513
                     /locus_tag="VYQ93_001414"
     CDS             9376..9513
                     /locus_tag="VYQ93_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336086.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEVLNIRLEVK
                     N"
     gene            9606..9667
                     /locus_tag="VYQ93_001415"
                     /pseudo
     CDS             9606..9667
                     /locus_tag="VYQ93_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
BASE COUNT         3107 a         1762 c         2113 g         2685 t
ORIGIN      
        1 ttaaccttat tttagatttg tgcaaacagt tattgttttt tgtctaaaaa tgttttagga
       61 taaaaatgtg attatcatgg ttctagagtt tgataaaaaa ctacagaata taaagcgctt
      121 tcttgaaaac aacaaaatca atcttttagg aggagaaaat gaagaatcca ttttttgaaa
      181 gacgttgtcg ttacagtatt cgtaagttat cagtaggagc ctgctcgctg atgattggtg
      241 ctgttttatt tgctggtcca gccttggctg aagaaactgc agttcctgaa aatagcggag
      301 ctaatacaga gcttgtttca ggagagagtg agcattcgac caatgaagct gataagcaga
      361 atgaagggga acatgctaga gaaaacaagc tagaaaaggc agaaggagta gcgatagcat
      421 ctgaaactgc ttcgccagca agcaatgaag ctgcaactac tgaaactgca gaagcagcta
      481 gcgcagctaa accagaggaa aaagcaagtg aggtggttgc agaaacacca tctgcagaag
      541 caaaacctaa gtctgacaag gaaacagaag caaagcccga agcaactaac caaggggatg
      601 agtctaaacc agcagcagaa gctaataaga ctgaaaaaga agtccagcca gatgtcccta
      661 aaaatacaga aaaaacatta aaaccaaagg aaatcaaatt taattcttgg gaagaattgt
      721 taaaatggga accaggtgct cgtgaagatg atgctattaa ccgtggatct gttgtcctcg
      781 cttcacgtcg gacaggtcat ttagtcaatg aaaaagctag caaggaagca aaagttcaag
      841 ccttatcaaa caccaattct aaagccaaag accatgcttc tgttggtgga gaagagttca
      901 aggcctatgc ttttgactat tggcaatatc tagattcaat ggtcttctgg gaaggtctcg
      961 taccaactcc tgacgttatt gatgcaggtc accgtaacgg ggttcctgta tacggtacac
     1021 tcttcttcaa ctggtctaat agtattgcag atcaagaaag atttgctgaa gctttgaagc
     1081 aagacgcaga tggtagcttc ccaattgccc gtaaattggt agacatggcc aagtattatg
     1141 gctatgatgg ctatttcatc aaccaagaaa caactggaga tttggttaaa cctcttggag
     1201 aaaagatgcg ccagtttatg ctctatagca aggaatatgc tgctaaggta aaccatccaa
     1261 tcaagtattc ttggtatgat gccatgacct ataactatgg acgttaccat caagatggtt
     1321 tgggagaata caactaccaa ttcatgcaac cagaaggaga taaggttccg gcagataact
     1381 tctttgctaa ctttaactgg gataaggcta aaaatgatta cactattgca actgccaact
     1441 ggattggtcg taatccttat gatgtatttg caggtttgga attgcaacag ggtggttcct
     1501 acaagacaaa ggttaagtgg aatgacattt tagacgaaaa tgggaaattg cgcctttctc
     1561 ttggtttatt tgccccagat accattacaa gtttaggaaa aactggtgaa gattatcata
     1621 aaaatgaaga tatcttcttt acaggttatc aaggagaccc cactggccaa aaaccaggtg
     1681 acaaagattg gtatggtatt gctaacctag ttgcggaccg tacgccagcg gtaggtaata
     1741 cttttactac ttcttttaat acaggtcatg gtaaaaaatg gttcgtagat ggtaaggttt
     1801 ctaaggattc tgagtggaat tatcgttcag tatcaggtgt tcttccaaca tggcgctggt
     1861 ggcagacttc aacaggggaa aaacttcgtg cagaatatga ttttacagat gcctataatg
     1921 gcggaaattc ccttaaattc tctggtgatg tagccggtaa gacagttcag gatgtgagac
     1981 tttattctac taagttagaa gtaactgaga agaccaaact tcgtgttgcc cacaagggag
     2041 gaaaaggttc taaagtttat atggcattct ctacaacttc agactacaaa ttcgatgatg
     2101 cagatgcatg gaaagagcta accctttctg acaactggac aaatgaagaa tttgatctca
     2161 gctcactagc aggtaaaacc atctatgcag tcaaactatt tttcgagcat gaaggtgctg
     2221 taaaagatta tcagttcaac ctcggacaat taactatctc ggacaatcac caagagccac
     2281 aatcgccgac aagcttttct gtagtgaaac aatctcttaa aaatgcccaa gaagcggaag
     2341 cagttgtgca atttaaaggc aacaaggatg cagatttcta tgaagtttat gaaaaagatg
     2401 gagacagctg gaaattacta actggctcat cttctacaac tatttatcta ccaaaagtta
     2461 gccgctcagc aagtgctcag ggtacaactc aagaactgaa ggttgtagca gtcggtaaaa
     2521 atggagttcg ttcagaagct gcaaccacaa cctttgattg gggtatgact gtaaaagata
     2581 ccagcctacc aaaaccacta gctgaaaata tcgttccagg tgcaacagtt attgatagta
     2641 ctttccctaa gactgaaggt ggagaaggta ttgaaggtat gttgaacggt accattacta
     2701 gcttgtcaga taaatggtct tcagctcagt tgagtggtag tgtggatatt cgtttgacca
     2761 agccacgtac cgttgttaga tgggtcatgg atcatgcagg ggctggtggt gagtctgtta
     2821 acgatggctt gatgaacacc aaagactttg acctttatta taaagatgca gatggtgagt
     2881 ggaagctagc taaggaagtc cgtggtaaca aagcacacgt gacagatatc actcttgata
     2941 aaccaatcac tgctcaagac tggcgcttga atgttgtcac ttctgacaat ggaactccat
     3001 ggaaggctat tcgtatctat aactggaaaa tgtatgaaaa gcttgatact gagagtgtca
     3061 atattccgat ggccaaggct gcagcccgtt ctctaggcaa taacaaggta caagttggct
     3121 tttcagatgt accggctgga gcaactatta ctgtttatga taatccaaat tctcaaactc
     3181 cgcttgcaac cttgaagagc gaagttggag gagacctagc aagtgcacca ttggatttga
     3241 caaatcaatc tggtcttctt tattatcgta cccagttgcc aggcaaggaa attagtaatg
     3301 tcctagcagt ttccgttcca aaagatgaca gaagaatcaa gtcagtcagc ctagaaacag
     3361 gacctaagaa aacaagctac gccgaagggg aggatttgga ccttagaggt ggtgttcttc
     3421 gagttcagta tgaaggagga actgaggacg aactcattcg cctaactcac gcaggtgtat
     3481 cagtatcagg ttttgatacg catcataagg gagaacagaa tcttactctc caatatttgg
     3541 gacaaccggt aaatgctaat ttgtcagtga ctgtcactgg ccaagacgaa gcaagtccga
     3601 aaactatttt gggaattgaa gtaagtcagg aaccgaaaaa agattaccta gttggtgata
     3661 gcttagactt gtctgaagga cgctttgcag tggcttatag caatgacacc atggaagaac
     3721 attcctttac tgatgaggga gttgaaattt ctggttacga tgctcaaaag actggtcgtc
     3781 aaaccttgac gcttcattac caaggccatg aagttagctt tgatgttttg gtatctccaa
     3841 aagcagcatt gaacgatgag tacctcaaac aaaaattagc agaagttgaa gctgctaaga
     3901 acaaggtggt ctataacttt gcttcatcag aagtaaaaga agccttcttg aaagcaattg
     3961 aagcggccga acaagtgttg aaagaccatg aaactagcac ccaagatcaa gtcaatgacc
     4021 gacttaataa attgacagaa gctcataaag ctctgaatgg tcaagagaaa tttacggaag
     4081 aaaagacaga gcttgatcgc ttaacaggtg aggttcaaga actcttggct gccaaaccaa
     4141 accatccttc aggttctgcc ctagctccgc ttcttgagaa aaacaaggcc ttggttgaaa
     4201 aagtagattt gagtccagaa gagcttacaa cagcgaaaca gagtctaaaa gatctggttg
     4261 ctttattgaa agaagacaag ccagcagtct tttctgatag taaaacaggt gttgaagtac
     4321 acttctcaaa taaagagaag actgtcatca agggtttgaa agtagagcgt gttcaagcaa
     4381 gtgctgaaga gaagaaatac tttgctggag aagatgctca tgtctttgaa atagaaggtt
     4441 tggatgaaaa aggtcaagat gttgatctct cttatgcttc tattgtgaaa atcccaattg
     4501 aaaaagataa gaaagttaag aaagtatttt tcttacctga aggcaaagag gcagtagaat
     4561 tggcttttga acaaacggat agtcatgtta tctttacagc acctcacttt actcattatg
     4621 cctttgttta tgaatctgct gaaaaaccac aacctgctaa accagcacca caaaacacag
     4681 tccttccaaa acctacttat caaccggctt ctgatcaaca aaaggctcct aaattggaag
     4741 ttcaagagga aaaggttgcc tttcatcgtc aagagcatga aaatgctgag atgctagttg
     4801 gggaacaacg agtcatcata cagggacgag atggactgtt aagacatgtc tttgaagttg
     4861 atgaaaacgg tcagcgtcgt cttcgttcaa cagaagtcat ccaagaagcg attccagaaa
     4921 ttgttgaaat tggaacaaaa gtaaaaacag taccagcagt agtagctaca caggaaaaac
     4981 cagctcaaaa tacagcagtt aaatcagaag aagcaagcaa acaattgcca aatacaggaa
     5041 cagctgatgc taatgaagcc ctaatagcag gcttagccag ccttggtctt gctagtttag
     5101 ccttgacctt gagacggaaa agagaagata aagattaaat atcgaaaaat cttgtgaaat
     5161 ctttccttat atttccaaag tgtgatataa tagtttcgaa taaaataaat aaaggagttt
     5221 ttgtaacatg gcaaaactta ctgttaaaga cgttgacttg aaaggtaaaa aagtcctcgt
     5281 tcgtgttgac ttcaacgtac cattgaaaga tggcgtaatc actaacgata accgtatcac
     5341 agcagctctt ccaactatta agtacatcat cgaacaaggt ggacgtgcaa ttcttttctc
     5401 tcaccttgga cgtgtgaaag aagaagctga taaagctggt aaatcacttg ctcctgtagc
     5461 agctgacttg gcagcaaaac ttggtcaaga tgttgttttc ccaggtgtca ctcgtggtgc
     5521 tgaattggaa gcggcaatca acgctcttga agatggacaa gttctcttgg ttgaaaacac
     5581 tcgttacgaa gatgttgacg gcaagaaaga atctaaaaac gatcctgaac ttggtaaata
     5641 ctgggcatca cttggagatg gtatcttcgt aaacgatgca ttcggtacag ctcaccgtgc
     5701 acacgcatct aacgttggta tctcagcaaa cgttgaaaaa gcagttgctg gtttccttct
     5761 tgaaaacgaa attgcctaca tccaagaagc agttgaaact ccagaacgtc cattcgtggc
     5821 tatccttggt ggttcaaaag tttcagacaa gatcggtgtt atcgaaaact tgcttgaaaa
     5881 agctgataaa gtccttatcg gtggtgggat gacttacaca ttctacaaag cacaaggtat
     5941 cgaaatcggt aactcacttg tagaagaaga caaattggat gttgcgaaag ctcttcttga
     6001 aaaagcaaat ggtaaattga tcttgccagt tgactcaaaa gaagctaacg catttgctgg
     6061 ttacactgaa gtgcgtgaca ctgaaggtga agcagtttct gaaggcttcc ttggtcttga
     6121 catcggtcca aaatctatcg ccaaatttga cgaagctttg actggtgcca aaacagtcgt
     6181 atggaacgga cctatgggtg tatttgaaaa cccagatttc caagctggta caatcggtgt
     6241 gatggacgct atcgtgaaac aaccaggagt taaatcaatc atcggtggtg gtgactcagc
     6301 tgccgcagcg attaaccttg gccgtgcaga caagttctca tggattagta cgggtggtgg
     6361 agcatcaatg gaacttcttg aaggtaaggt tcttccagga cttgcagcct tgacagaaaa
     6421 ataagatttt ataaataaat caaagaagag agggatgaaa gttcctcttt tcttttgctt
     6481 aaaataaaaa cgcttcctct caactattac tcataaaaat caccgattta tgataaaatg
     6541 gaaatagaaa gttgagatta tgagttattt taaaaaatat aaattcgata aatcccagtt
     6601 caaacttggt atgcgaacct ttaaaacagg tattgctgtt tttctagttc tcttgatttt
     6661 tggctttttt ggctggaaag gtcttcaaat tggtgctttg acagccgttt ttagcctgag
     6721 ggagagtttt gatgagagtg ttcattttgg gacttcgcgt attctaggaa atagtatcgg
     6781 tggactctat gccttggtct tcttcttatt aaataccttt ttccacgaag ccttttgggt
     6841 gaccttggta gttgttccaa tctgcaccat gttaaccatt atgacaaatg tagccatgaa
     6901 taacaaagca ggggttattg gtggtgtagc agctatgtta atcattaccc tatcaattcc
     6961 aagtggtgag acaattttgt acgtgtttgt gcgtgtatta gaaacgttta tgggagtttt
     7021 tgtcgcaatt atcgtaaatt acgatattga tcgtattcgt ctctttttag agaaaaaaga
     7081 aaaataatgt tacattttat aacattatca attgacgttt gtcttttttt agactataat
     7141 agacagaaag aaggaaattg taaatgaagg aaaaagaatt tcgccgaaat atggctgttt
     7201 ttcctatcgg cagtgttatg aagttgaccg atctatcggc gcgtcagatt cgttattatg
     7261 aagatcaaga gttgatcaag cccgatcgaa acgaaggaaa tcgtcgcatg tattccttga
     7321 atgacatgga tcgtctgctt gaaatcaaag attatatctc tgaaggttat aatatcgctg
     7381 ccattaagaa aaaatatgct gaacgtgaag cgaaatccaa gaaagcggtt agtcagactg
     7441 aagtacgtcg tgcacttcac aatgaactcc tccaacaggg gcgttttgct tcagtacagt
     7501 caccttttgg tcgcggttag gcaaccgcaa gtagtcatag attaaggaga aaacttatgc
     7561 caatcacagc tgcagatatt cgtcgtgaag tcaaggaaaa aaatgttacc tttattcgtc
     7621 ttatgttctc agatattttg ggaaccatga aaaacgtcga aattcctgct acagatgaac
     7681 agttagataa ggtcttgtcg aacaaggtta tgtttgatgg atcttctatt gaaggttttg
     7741 tacgtatcaa tgagtcggat atgtacttgt acccggactt ggatacatgg acagtcttcc
     7801 cttggggaga tgaaaatgga agtgttgcag gtctgatctg tgatgtctat acaacagaag
     7861 gtgaaccatt tgcgggtgac cctcgtggta atttgaaacg agctcttcgt cacatggaag
     7921 aagttggatt caaatccttc aaccttggtc cagagccaga attcttccta tttaagttgg
     7981 atgaaaatgg ggacccaaca cttgaagtga atgacaaggg tggctacttt gatttggcac
     8041 ctactgacct tgcggacaac acacgtcgtg agattgtgaa tgtcttgacc aaaatgggat
     8101 ttgaagtaga agcgagtcac cacgaggttg cggttggaca gcatgagatt gactttaagt
     8161 acgatgaagt tctccgtgct tgtgataaga ttcaaatctt taagcttgtt gttaaaacca
     8221 ttgctcgcaa acacggactt tacgcaacat ttatggcgaa gccaaaattt ggtattgctg
     8281 gatcaggtat gcactgtaat atgtccttgt ttgatgcaga aggaaataac gccttctttg
     8341 atccaaatga tccaaaagga atgcagttgt cagaaacagc ttaccatttc ctaggcggtt
     8401 tgatcaagca tgcttacaac tatactgcca tcatgaaccc aacagttaac tcatacaaac
     8461 gtttggttcc aggttatgaa gcgcctgttt acattgcttg ggctggtcgt aaccgttcgc
     8521 cacttgtgcg cgtacctgct tcacgtggta tgggaactcg tcttgagttg cgttcagtgg
     8581 atccaatggc gaacccttac gttgctatgg ctgttctttt ggaagttggt ttgtatggta
     8641 ttgaaaataa aatcgaagca ccagctccta tcgaagaaaa tatctacatc atgacagcag
     8701 aagagcgcaa ggaagctggt attacagacc ttccatcaac tcttcacaac gctttgaaag
     8761 ctttgacaga agatgaagtg gttaaagctg ctctcggaga tcacatctat actagcttcc
     8821 ttgaagccaa acgaatcgaa tgggcaagtt atgcaacctt cgtttcacaa tgggaaattg
     8881 ataattattt agacctttac taatactaat atagaagaaa gattgcctgt gggtaaacag
     8941 tcacacagca gtctaagcca tcaaatacct ataaccctta aaatagcgat ttcggtcgct
     9001 atttattttt cgaaaaatcc tcttgactct tttgcgaatt agatttatca tcagacgtgt
     9061 agaagaaaat taaggagtca ctatggaaaa attcaatttc aaaaacaata tcgggcaaga
     9121 aaacaagtta cttcaaatcg aaatctacaa gtttactaac ttttgcaagc ttcaaaatta
     9181 cacatctgta aatatttttt cgaaagatat atttgaagcc atagtaaatt gagactagaa
     9241 tagtacacta catctgctaa aaatttctag aaactaattt aactttccta atcgatttgt
     9301 ttacatctta tttcaatcta ctatattatc tcaataaatg atgatggcga ctgttttgtt
     9361 tgtgcttgtc gatttttgaa tcctgatcga gcggaagaat atttctgccg aggttgccaa
     9421 ggggaaaatc ctgaagatat tgaattttat gatgaacaat tacaggcaga aaaggtggag
     9481 gttttgaata ttcgattgga agtaaaaaat tgaattatct acaatctcat caacaataaa
     9541 cctatttcaa ccccattatc tataggcgta tttttacgcc tttttatata cttgaagcac
     9601 ataagaaaca ttatgggaag tttgctgatg gaagtactca agaaattgat gttccttatg
     9661 atattcc
//
LOCUS       DAVMGZ010000075         9520 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000075 DAVMGZ010000000
VERSION     DAVMGZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.076"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            44..1717
                     /locus_tag="VYQ93_001416"
     CDS             44..1717
                     /locus_tag="VYQ93_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV3336087.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEQEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            1829..2920
                     /gene="ulaG"
                     /locus_tag="VYQ93_001417"
     CDS             1829..2920
                     /gene="ulaG"
                     /locus_tag="VYQ93_001417"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV3336088.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            3034..5010
                     /gene="tkt"
                     /locus_tag="VYQ93_001418"
     CDS             3034..5010
                     /gene="tkt"
                     /locus_tag="VYQ93_001418"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV3336089.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            5127..5426
                     /gene="yajC"
                     /locus_tag="VYQ93_001419"
     CDS             5127..5426
                     /gene="yajC"
                     /locus_tag="VYQ93_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV3336090.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            5472..5900
                     /locus_tag="VYQ93_001420"
     CDS             5472..5900
                     /locus_tag="VYQ93_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV3336091.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNVSDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            5902..6312
                     /locus_tag="VYQ93_001421"
     CDS             5902..6312
                     /locus_tag="VYQ93_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336092.1"
                     /translation="MENLTNFYEKYRVYLTRPRLEILTVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            6612..9263
                     /gene="adhE"
                     /locus_tag="VYQ93_001422"
                     /gene_synonym="adhC"
     CDS             6612..9263
                     /gene="adhE"
                     /locus_tag="VYQ93_001422"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV3336093.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         3040 a         1761 c         1987 g         2732 t
ORIGIN      
        1 aaagaattgc tgtgatatca atagatttgg gggatttttt aatatggtac tggataaggc
       61 aagttgtgat ttacttcaat atttgatgga tcaagaaacg tccaaaacga ttatggcgat
      121 ttcgaaagat ttgaaagagt caagaaggaa aatttattat cacattgaca aaatcaatgc
      181 tgctctgggt gacgaggcgc ttcacatcat tagtattcca cgaattggta ttcacttaac
      241 ggaagagcag agagatgctt gttgtaaact attatcggaa gtagattcgt acgattatat
      301 catgagtgca catgaacgta tgatgataat gttactatgg ataggtattt ctaaagaacg
      361 tattacgatt gaaaaattga tagagttaac agaggtatct aggaatactg ttctcaatga
      421 tttgaatagt attcgttatc aactaacttt ggaacaatat caggtgatct tgcaagtgag
      481 caagtcacag ggataccacc ttcatgccca ccctcttaat aaaattcagt atcttcaatc
      541 gcttctatat catattttta tggaagaaaa tgccactttt gtatctattt tagaagataa
      601 gatgaaagag aggttagatg atgagtgttt gctttctgtt gaaatgaacc aattttttaa
      661 ggaacaggtt cctttagttg aacaagattt agggaagaaa ataaaccatc atgaaataac
      721 ttttatgttg caggttctac cttatttgct gttaagctgt cataatgttg aacagtatca
      781 agaaagacat caggatatag agcaagaatt ttctttgata agaaaaagaa tagagtatca
      841 ggtgtctaag aagttaggag aacggttgtt tcaaaagttt gaaatttctt tgtcaggact
      901 tgaagtttct cttgtagctg ttctcctcct ctcctatcgt aaagatttgg atattcatgc
      961 agaaagtgat gattttcggc aattaaaact tgctttagaa gaatttatct ggtattttga
     1021 atcacaaatc cgaatggaga ttgagaacaa ggatgatttg ttacgaaatt tgatgatcca
     1081 ctgtaaagcc ttgttattta gaaagactta cggtattttt tctaaaaatc ctctaacaaa
     1141 acaaattcga tccaagtatg gagaattatt tttagtcact agaaaatctg cggaaatttt
     1201 agaaggagca tggtttattc ggctaacaga cgatgatatt gcctatttga cgattcatat
     1261 tggaggattt ttaaaatata caccatcgtc tcaaaaaaat atgaaaaaag tttatctcgt
     1321 ttgtgatgaa ggtgttgcgg tttcgagact tttgctgaaa caatgcaaac tttattttcc
     1381 aaatgagcaa attgacactg tatttacaac agaacaattt aagagtgtgg aagatattgc
     1441 acaagttgat gtagtgatta ctactaatga tgatttggat agcagatttc cgattttaag
     1501 ggttaatcct atccttgaag cagaagatat tttgaaaatg ctagactatc ttaaacacaa
     1561 tatatttcgt aataagagca aaagtttcag tgaaaatctt tctagtctta tttcgtctta
     1621 tattgtagac agcaagttgg ctagtaagtt ccaagaagag gttcaaacac ttataaatca
     1681 agaaatagta gttcaagctt ttttggaaga tatttgaagg acagtccaat gatgaacaca
     1741 aacctgtgtt tttcttggtc ttttttagtg ttttgaaggg tggtatacta atctcaaaga
     1801 taacaattat atccaaagga ggcaacatat gccaaacgtc aaagaaatta caagagagtc
     1861 atggatttta gccactttcc cagagtgggg aacatggttg aacgaagaaa tcgaagaaga
     1921 agtcgtacct gaaggcaact ttgccatgtg gtggctaggc aactgtggta cttggattaa
     1981 gacaccagct ggtgctaacg ttgtcatgga cctttggtca aaccgtggaa aatcaaccaa
     2041 aaaagtgaaa gatatggttc gtgggcacca aatggcaaat atggcaggtg ttcgtaagtt
     2101 gcaaccaaac ttgcgtgttc agccaatggt tatcgatcca tttgctatca acgaactaga
     2161 ctattactta gtttcacact tccacagtga tcatatcgac ccatacacag ctgcagcaat
     2221 tctcaacaat cctaagttag agcatgttaa gtttatcggt ccttaccact gtggacgaat
     2281 ctgggaagga tggggtgttc caaaagaacg tatcatcgtt gttaaaccag gtgacactat
     2341 cgaattaaaa gatatgaaga ttcatgcagt agaatcattt gaccgtactt gcttggtaac
     2401 tctcccagtg aacggtgctg atgagacagg cggtgaactt gctggcttgg ctgttacaga
     2461 tgaagaaatg gctcaaaagg ctgttaacta tatctttgaa acaccaggtg gaaccatcta
     2521 tcatggtgca gattctcact tctcaaacta ttttgcaaaa catggtaaag actttaaaat
     2581 tgatgttgct ttgaataact atggtgaaaa tccggtaggt atccaagaca aaatgacatc
     2641 tatcgacctt cttcgtatgg cagaaaatct gcgtaccaaa gtcattatcc cagttcacta
     2701 tgatatctgg tctaacttca tggcttctac taatgagatt ctagaacttt ggaaaatgcg
     2761 aaaagatcgc ttgcaatacg atttccatcc atttatctgg gaagttggcg gtaagtacac
     2821 ttatcctcaa gatcaacact tagtagaata ccatcatcca cgtggttttg atgattgttt
     2881 tgaacaagac tctaacattc aatttaaagc tttgctataa tataaaaata ttctttggag
     2941 gttatccgta tgttgcatga tacggataat tttcatataa tagtaaaata gaatgtgtga
     3001 ttcaataatc acctcaaata gaaaggaaat tctatgtcaa atctatctgt taatgcaatt
     3061 cgttttctag gtattgacgc cattaataaa gccaactcag gtcatccagg tgtggttatg
     3121 ggagcggctc cgatggctta cagcctcttt acaaaacaac ttcatatcaa tccagctcaa
     3181 ccaaactgga ttaaccgcga ccgctttatt ctttcagcag gtcatggttc aatgctcctt
     3241 tatgctcttc ttcacctttc cggttttgaa gatgtcagca tggatgagat taagagtttc
     3301 cgtcaatggg gttcaaaaac accaggtcac ccagaatttg gtcatacggc agggattgat
     3361 gctacgacag gtcctctagg gcaagggatt tcaactgcta ctggttttgc ccaagcagaa
     3421 cgtttcttgg cagccaaata taaccgtgaa ggctacaata tctttgacca ctatacttac
     3481 gttatctgtg gagacggaga cttgatggaa ggtgtctcaa gcgaggcagc ttcatacgca
     3541 ggtttgcaaa aacttgataa gttggttgtt ctttatgatt caaatgatat caacttggat
     3601 ggtgagacaa aggattcctt tacagaaagt gttcgtgacc gttacaatgc ctacggttgg
     3661 catactgcct tggttgaaaa tggaacagac ttggaagcca tccatgctgc tatcgaaaca
     3721 gcaaaagctt caggcaagcc atctttgatt gaagtgaaga cggttattgg atacggttct
     3781 ccaaacaaac aaggaactaa tgctgtacac ggcgcccctc ttggagcaga tgaaactgca
     3841 tcaactcgtc aagccctcgg ttgggactac gaaccatttg aaattccaga acaagtatat
     3901 gctgatttca aagaacatgt tgcagaccgt ggcgcatcag cttatcaagc ttggactaaa
     3961 ttagttgcag attataaaga agctcatcca gaactggctg cagaagtaga agccatcatc
     4021 gacggacgtg atccagtcga agtgactcca gcagacttcc cagctttaga aaatggtttt
     4081 tctcaagcaa ctcgtaattc aagtcaagat gccttgaatg ttgtggcagc taaattacca
     4141 accttcctag gtggatcagc tgaccttgct cactcaaaca tgacttatat caagacggat
     4201 ggacttcaag acgatgccaa tcgcttgaat cgtaacattc agtttggtgt tcgtgaattt
     4261 gcaatgggaa caatcttgaa cgggatggcc cttcatggtg gacttcgtgt atacggtgga
     4321 actttcttcg tcttctctga ctatgtgaag gcagctgtcc gcttgtcagc cttacaagga
     4381 cttcctgtga cttatgtctt tacccatgat tcaatcgcag ttggggaaga tggtccgact
     4441 cacgaaccag ttgagcattt agcaggtctt cgtgctatgc caaatctaaa tgttttccgt
     4501 ccagcagatg cgcgtgaaac gcaagcagct tggtaccttg cagtgacaag tgagaaaaca
     4561 ccaactgccc ttgtcttgac acgtcaaaac ttgactgttg aagatggaac agacttcgac
     4621 aaggttgcta aaggtgctta tgttgtatat gaaaatgcag ccgactttga taccatcttg
     4681 attgcgacag gttcagaggt taatcttgct gtctcagctg ctaaagaatt ggctagtcaa
     4741 ggcgaaaaaa tccgcgtagt cagcatgcca tctacagatg tctttgataa acaagatgca
     4801 gcttacaagg aagaaattct tccaaatgca gtccgccgtc gtgttgcagt cgaaatgggt
     4861 gcaagtcaaa actggtacaa atatgttggt ctcgatggtg ccgttctagg tattgatacc
     4921 ttcggagcct ctgccccagc accaaaagta ttggcagaat atggctttac tgtcgaaaat
     4981 cttgtaaaag ttgttcgaaa tttgaaataa tcctaaaaat cagggcgtaa gctctggttt
     5041 ttcttaccag aaaagtaagg tacaatcttg taaaagaagc tgaaatttga tatagtagtc
     5101 ctatgtaaaa gacaaaggag aaaacaatga atccaaatat tactttttta atcatgcttg
     5161 taggtatgat ggccttgatg ttctttatgc aacgttctca aaagaaacaa gctcaaaagc
     5221 gtatggaaag cttaaataaa ctacaaaaag gctatgaagt gattacaatc ggtggacttt
     5281 atggaacagt cgatgaagta gatacggaga agggaacaat agttcttgac gtagatggag
     5341 tttacttgac ttttgaacta gctgctatca agacagtatt accgctgaaa gaaacagctt
     5401 cactagaagg cgcaattgaa aaataagacg ggatcacgaa ctcccgtttt ctataaaaga
     5461 aaggaaatgg gatgaaaaaa ttagtctttg tctgtctggg aaatatttgc cgtagcccta
     5521 tggccgagtt tgttatgaaa tcaatgacag ataactacga aatccaaagt cgagcaactt
     5581 cctcttggga acatggcaat ccgattcata aggggactca gggaattttt caagagtatg
     5641 agattcctta tgacaagaac aagacatcgc ttcagattag taaggaagat tttgaagcct
     5701 ttgattatat tatcggaatg gacgcttcaa atgtttccga cttacgtcag atgtgtccag
     5761 tagactgtca agataagatt tactcatttt catctgaaag tgttccagac ccttggtata
     5821 caggagattt tgaagaaacc tatcgacgtg ttcaagaggg ctgtcaagct tggttagaac
     5881 gtttagaaaa ggagagttaa ggtggaaaat cttacgaatt tttacgaaaa gtatcgtgtt
     5941 tatctgactc gtccacgttt agagattttg acagtagtta ccattgtttt ctgtgctgta
     6001 ctcgtctttt ttctaaatat tccaggaaaa ggtgtcttaa aactcgataa tggaacgatt
     6061 gtttatgatg gcagtcttgt ccgcggtaaa atgaatggcc aaggtaccat taccttccaa
     6121 aatggagacc aatatacagg tggcttcaac aatggagcct tcaacggaaa aggtaccttt
     6181 caatctaaag aaggctggac ctacgaaggt gattttgtaa atggtcaggc tgaaggaaaa
     6241 gggaaactaa caacagaaca agaagtcgtt tatgaaggaa cttttaaaca aggcgttttt
     6301 caacaaaaat aaagcctcct tatcaaagga ggtattatta gaattacaag gtaagcgctt
     6361 acctgtaaat ccctttcttt ccaaatccct cttccaagca agtttgtgaa ataaaaaata
     6421 tttgaaataa atttcacaaa ctttaaagat aaaacctgat aagaaaagaa aatgagaaaa
     6481 gtttcacaag agtttaaaaa ttctttgtga aatgtttatg aaactgttta caaagttgaa
     6541 ataatgcgtt ataataaact tgtgaaaaaa ttaacaaagg atatcgatcc ttgaaagcta
     6601 tggaggaaaa tatggctgat aaaaaaactg tgacaccaga ggaaaagaaa ctcgttgctg
     6661 aaaaacacgt agatgagttg gttcaaaaag ctctagttgc ccttgaagaa atgcgtaaat
     6721 tggatcaaga gcaagttgac tacatcgttg ccaaagcatc agtagcagct ttggatgccc
     6781 acggagaatt ggctttacat gcctttgaag aaacaggacg tggtgtattt gaagataaag
     6841 caactaagaa cttgtttgct tgtgaacacg tagtaaacaa catgcgccac actaagacag
     6901 ttggcgttat cgaagaagac gatgtaacag gattgactct tattgctgaa ccagttggtg
     6961 ttgtttgtgg tattactcca acaacaaacc caacatcaac agcaatcttc aaatcattga
     7021 tttcattgaa gacacgtaac ccaatcgtct ttgccttcca tccatcagca caagaatcat
     7081 ctgctcatgc agctcgtatc gtccgcgatg cagctatcgc agctggtgct cctgaaaact
     7141 gtgtgcaatg gattactcaa ccatctatgg aagcaacaag tgcccttatg aaccacgaag
     7201 gtgttgcgac aatccttgca acaggtggta atgccatggt taaggcggct tattcatgtg
     7261 gtaaaccagc tcttggggta ggtgccggaa acgttccagc ttatgttgaa aaatcagcaa
     7321 acattcgtca agcagcacac gatatcgtca tgtctaaatc atttgataac ggtatggtct
     7381 gtgcatctga acaagcagtt atcattgata aagaaattta cgatgaattt gtagcagagt
     7441 tcaaatctta ccacacttac tttgtaaaca aaaaagaaaa agctcttctt gaagagttct
     7501 gcttcggcgt caaagcaaac agcaaaaact gtgctggtgc aaaattgaac gctgacatcg
     7561 ttggtaaacc agcaacttgg attgcagaac aagcaggatt tacagttcca gaaggaacaa
     7621 acattcttgc tgcagaatgt aaagaagttg gcgaaaatga gccattgact cgtgaaaaat
     7681 tgtcaccagt tattgcagtt ttgaaatctg aaagccgtga agatggtatt actaaggctc
     7741 gtcaaatggt tgaatttaac ggtcttggac actcagcagc tatccacaca gctgacgaag
     7801 aattgactaa agaatttggt aaagctgtta aagctattcg tgttatctgt aactcacctt
     7861 ctacttttgg tggtatcggg gacgtttaca atgccttctt gccatcattg acacttggat
     7921 gtggttctta cggacgcaac tcagttgggg ataacgttag tgccattaac ctcttgaata
     7981 tcaaaaaagt cggaagacgg agaaataaca tgcaatggat gaaacttcct tcaaaaacat
     8041 actttgaacg tgattcaatt caataccttc aaaaatgtcg tgacgttgaa cgtgtcatga
     8101 tcgttactga ccatgccatg gtagagcttg gtttccttga tcgtatcatc gaacaactgg
     8161 accttcgtcg caataaggtt gtttaccaaa tctttgcgga tgtagaaccg gatccagata
     8221 tcacaactgt aaaccgtggt actgagatta tgcgtgcctt caaaccagat accatcatcg
     8281 cactcggtgg tgggtctcca atggatgctg ccaaagtaat gtggctcttc tacgagcaac
     8341 cagaagtgga cttccgtgac cttgtccaaa aattcatgga tatccgtaaa cgtgccttca
     8401 agttcccatt gcttggtaag aagactaaat tcatcgcgat tccaactaca tctggtacag
     8461 gatctgaagt aacaccattt gccgttatct ctgataaagc aaacaaccgt aaatacccaa
     8521 tcgctgacta ctcattgaca ccaactgtgg caatcgtaga tcctgctttg gtattgacag
     8581 ttccaggatt tgttgctgct gatactggta tggacgtatt gactcacgcg acagaagcat
     8641 acgtatcaca aatggctagt gactacactg atggtttagc acttcaagct attaaattgg
     8701 tctttgaaaa tctcgaaagc tcagttaaaa atgcagactt ccactcacgt gagaaaatgc
     8761 ataacgcttc aacaatcgct ggtatggcct ttgccaatgc cttcctaggt atttctcact
     8821 caatggccca taagattggt gcgcaattcc acacagttca cggacgtaca aatgccatct
     8881 tgcttccgta cgttatccgt tacaacggta cacgtccagc taagacagca acatggccta
     8941 agtacaacta ctaccgcgca gatgaaaaat accaagatat cgcacgcatg cttggacttc
     9001 cagcttctac tccagaagaa ggggttgaat cttacgcaaa agctgtctac gaactcggtg
     9061 aacgtattgg gatccaaatg aattttagag accaaggaat tgacgaaaaa gaatggaaag
     9121 aacattctcg tgaattagcc ttcctggctt atgaagacca atgttcacca gctaacccac
     9181 gtcttccaat ggtagaccat atgcaagaaa tcatcgaaga tgcatactat ggctacaaag
     9241 aaagaccagg acgccgtaaa taattgttta tcagtctaga agcaagacaa aaactcaatt
     9301 tgagggaaag atccagtaat ttttctatga taaaaggcat cctatcaagg tttttgaaca
     9361 cctgatagga tgccttttta tgatattgag gcctttttgc cctttttgaa aaactagaat
     9421 agaaacaaaa tatataatag attgaaacta gagtactaca catctgcttc taaaacattg
     9481 ttagaattcg atttgactgt cctgatcaat ttgtcctgtt
//
LOCUS       DAVMGZ010000076         9456 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000076 DAVMGZ010000000
VERSION     DAVMGZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.077"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..155
                     /locus_tag="VYQ93_001423"
     CDS             <1..155
                     /locus_tag="VYQ93_001423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336094.1"
                     /translation="MKIKEQTRKLAAGYSKHSFEVVDETDEVSNHTYGKATLTWFEEI
                     FEEYKN"
     gene            complement(236..2059)
                     /gene="lepA"
                     /locus_tag="VYQ93_001424"
     CDS             complement(236..2059)
                     /gene="lepA"
                     /locus_tag="VYQ93_001424"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV3336095.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(2138..2866)
                     /locus_tag="VYQ93_001425"
     CDS             complement(2138..2866)
                     /locus_tag="VYQ93_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV3336096.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            complement(2868..4535)
                     /gene="recN"
                     /locus_tag="VYQ93_001426"
     CDS             complement(2868..4535)
                     /gene="recN"
                     /locus_tag="VYQ93_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV3336097.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKERIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            complement(4542..4973)
                     /locus_tag="VYQ93_001427"
     CDS             complement(4542..4973)
                     /locus_tag="VYQ93_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV3336098.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            complement(4966..5781)
                     /locus_tag="VYQ93_001428"
     CDS             complement(4966..5781)
                     /locus_tag="VYQ93_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV3336099.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQIFAEIKEAVERAHSQFKNE"
     gene            complement(5774..6649)
                     /locus_tag="VYQ93_001429"
     CDS             complement(5774..6649)
                     /locus_tag="VYQ93_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV3336100.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQIIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            complement(6646..6858)
                     /locus_tag="VYQ93_001430"
     CDS             complement(6646..6858)
                     /locus_tag="VYQ93_001430"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV3336101.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            complement(6836..8176)
                     /locus_tag="VYQ93_001431"
     CDS             complement(6836..8176)
                     /locus_tag="VYQ93_001431"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV3336102.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEKGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            complement(8304..8942)
                     /gene="udk"
                     /locus_tag="VYQ93_001432"
     CDS             complement(8304..8942)
                     /gene="udk"
                     /locus_tag="VYQ93_001432"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV3336103.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            complement(9050..9352)
                     /locus_tag="VYQ93_001433"
     CDS             complement(9050..9352)
                     /locus_tag="VYQ93_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV3336104.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQVPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
BASE COUNT         2659 a         2301 c         1723 g         2773 t
ORIGIN      
        1 caatgaaaat caaagagcaa actaggaagc tagccgcagg ttactcaaaa cacagttttg
       61 aggttgtaga tgaaactgac gaagtcagta accatacata cggtaaggcg acgctgacgt
      121 ggtttgaaga gattttcgaa gagtataaaa actaaaaaag cagaccatct aagcctgctt
      181 tactattgat tcttatataa atttcctgtg aacaaggaaa ggcatttctg ataacttatt
      241 cttcatccat actcaagacg ctgaggaagg cttcttgcgg aacttcaact gatccgatgg
      301 atttcatgcg tttcttacca gctttttgtt tttcaaggag tttacgctta cgagaaacgt
      361 caccaccata acatttagca agtacgttct tacgaagggc cttgatatca gtacgagcga
      421 caatcttgtg tccaatagcc gcttggattg gaacctcaaa ttgttggcga gggatgattt
      481 tcttgagctt atcaacgatg agtttcccac gttcataagc aaagtcctta tgaacgataa
      541 agctgagggc atccaccttg tctccgttga gaaggatatc cattttcact agcttagatg
      601 ggcgatattc tgacaattcg tagtcaaaac ttgcataacc acgtgtcgaa gacttaagtt
      661 tatcaaagaa gtcaaagacg atttcagcaa gaggaatttg atagataaca ttgacacggt
      721 tatcatcaat atagtccata gtcacaaagt ccccacgctt acgctgagct agctccatta
      781 ctgctccgac gaactcctgt ggtaccatga tttgcgcctt gacataaggc tcttcaatgg
      841 tcgcaatctt agttgggtct ggaaactcag atgggttaga cacatccata gactcaccgt
      901 cggtcaaatt aactttgtaa ataacagacg gagctgtcat gatgagatca atattgaact
      961 cacgctctaa acgttcctgg ataacatcca tatggagaag tccaagaaat ccacaacgga
     1021 aaccaaatcc aagtgcctga gatgtttctg gttcaaactg aagactagca tcattcagtt
     1081 gcaatttttc aagcgcttca cgcaggtcat tgtacttgtt tgattcgatt gggtagagac
     1141 ccgcaaagac cataggattc atctgcttat aaccatgtaa tggttctgcc gcaggattgg
     1201 ttgccaaggt aacggtatca cccacacgag tatcctgaac cgtcttgata gacgccgcaa
     1261 tgtaaccaac atcaccagtc gcaaggaaat cacgaccaac cgcttttggt gtaaaaatac
     1321 cgacttcggc cacatcaaag gtcttactat tgctcatgag ctgaatctta tcaccaggtt
     1381 tgaccactcc gtccatgaca cgcacttgga ggataacccc acggtaagca tcgtaaacag
     1441 agtcgaaaat caaggcctta agtggcgccg tcacatcacc cgttggtgct ggtacttttt
     1501 ctacaatttg ctcgaggatt tcttcaatcc caataccagc cttggcagaa gccaaaactg
     1561 cttcactggc atccaaacca atcacatctt caatctctgt acgcacgctc tccggatctg
     1621 cagccggcag gtcaatttta ttaatgatag gcatgatttc caaatcatta tccaaagcca
     1681 gataaacgtt ggcaagagtt tgagcctcaa ttccttgagc cgcatcgacc accaaaatag
     1741 caccctcaca ggcagctagc gaacgtgaaa cttcataggt aaagtcaacg tgccctggtg
     1801 tgtcaatcaa gtggaaaata taagtttccc catcttttgc agtgtaattc aactcgatgg
     1861 cattcaactt aatagtaatt ccacgttccc gctctagatc catgctatcc aaaagctggg
     1921 cctgcatttc acgacttgaa accgtctctg ttttttccaa aatgcggtct gctagagttg
     1981 atttcccgtg gtcaatatgg gcgataatag agaagttacg gatcttcccc tgtcgttttt
     2041 tcaattcttc taagttcatg attctcttcc tttcagggta tctatttatt ataaattgtt
     2101 tttgatattt tgacaagacc ataccctgct aggagtacta atcttcagcg acaaagccgt
     2161 cattttcgat aaagtggtgt tctgtcattc cttggtctgt aaagacaatc ccgtgaagga
     2221 caccaccata aacagctcct ccatccattc caatcttgcc atcttctgta gtccaaagct
     2281 cagatgtacc gcgttcttgc tgtaacaaac catagaccgg tgtatgaccg aagacaatgg
     2341 tttttccagt atgattttca gctccgtgga atggttttct aagccatact tttttataat
     2401 ctgttgtttc atgccagtcg tccaaagtca aatcaatacc tgcgtgaaca aatatatact
     2461 tgtctgtctc tactacaaat ggcatttgac gaatgaattc gaccaagtct gacgcttcag
     2521 cggcaacccg cttggcatct tctactccat caactggtgc atccaaggga cgacctagga
     2581 tagagttaat ggttgtatct ccaccattgc gacgataatg gtcataactt tcttctgggt
     2641 catctagcca agtcaaaaac atatactcgt ggtttccaga caaacagata gccccttgat
     2701 tgtccaccaa gtccttgacc atttcaagaa cacggtgact atcctcacct ctgtcaatca
     2761 aatcacctag aaagagcaac tgggtctgac catcccaggt tttaagaagg tcttccagca
     2821 tcccagcttt tccgtgaaca tctccaatta cataataatc tgtcatctta tttctccctg
     2881 tttctcaaca attctctggc ttgcgtcagg gctgcttctg tcacatcatc acctgccaac
     2941 atcttggcaa cttcctccac tcgctcttcg actgtcaaga gacgaacagt cgaaaccgtt
     3001 gaatggtcat tactaatctt ctcaataaag aattgataat ctgcaatcgc aattacttgt
     3061 ggcaaatggg agatagccaa aacctgacca tgctgaccaa ttttatgaat tttctgagca
     3121 atagcttgag caacacgacc tgaaactccc gtatccacct catcaaagac aatgctagtc
     3181 ttgccttctt tacgtgaaaa ggcagactta atggctaaca tgagacgaga taattcccct
     3241 ccagaagcaa ccttaaccaa gggtttaaag tcttctccag ggttggttga aatataaaac
     3301 tcaaccattt catttccccc acgactgaat tttcccttac taaaatgaac ctgaaactgg
     3361 gctttttcca tataaagatc ttgcagttct tgtttaatct cagcttcgag ttgctgagcc
     3421 aaattatgac gagcagaagc aagttgacct gccaaattga caagattgac ttccaacttc
     3481 ttaagctctg cttccatgtc ctcagacgaa agattattgc ctgtcaagag attgtattct
     3541 tccgtaatct tggcaaaata aagcaaaaca tcatcaacag tcccaccata cttacgagta
     3601 atagtatgaa ggaggtccaa acgattctca acctgcatca ggcgattgcc atcaaaatca
     3661 aggtcctcaa tgatagcttc caaacgtttg ctaatgtctt ctaaaacata gtaggtctca
     3721 gacagagagc ttgaaatttc acggtattca ggatcatact cttcgacact ttccatgtca
     3781 ttcatagctg aacgaacatt ggccagactt gaaaaatctt cattgtccaa catactgtag
     3841 gcattggtca gtgtatccgc aatatttttg tggttgagga gtttatctcg ctcttgattg
     3901 agagccaagt cttctccagc ctgcaagttt gctgcctcaa tctctgccat ttgaaattcc
     3961 aacatttcga tacgttcctt gtgttcctgt tggtttttct tgacttccag aacctgcttg
     4021 cgcattttcc gataggcatc aaaactcgtt tgataggttt ctttcaagtc ccaaaaagcg
     4081 gcatcaccaa attcatccaa catctggata tgcagttggg gacgcattaa ctcctcatgg
     4141 tcatgctgac catgaatatc tacaagatgt tgcccaatag ctcgcaaaac agacagatta
     4201 accatctgac catttacacg gctgatacta cgaccatttt gcaagatttc ccgacggatg
     4261 ataatttcat cacctaattc taaaccttgc tcatcaaaaa tttcctgtaa aagacgacta
     4321 ttctcaactg agaaaagccc ctcaatctct gcctttggtg caccatgacg aataacatct
     4381 gtcgtcgcac gagctcccaa catcatattc atggcatcaa tgataatcga cttccctgca
     4441 cccgtttcac cagtcaggac agtcatcccc ttttcaaaat tgagggaaat agcctcaata
     4501 atggcaaagt tttttatcga aatttcaagt aacatataga cctaccaatt ttttacttgt
     4561 tcaaagattt cctctgctag acttccactt ctggcaatga ctaaaatcga gctatcatca
     4621 gtcaaacaac taaaaatctt gtctgcaaaa gtatcgatta actgagcttt tacaaaagcc
     4681 gtatttcctg gaataacttg gagattgatc atcttatcca tcaattcagc cgattcgata
     4741 ttgtcttcag ccagttgcag actttttacg attgattttg gtaattcgta gacataggtg
     4801 ttgtctctca aaggaatttt gacaatacct aactctttga tatctcggga taccgtcgcc
     4861 tgagtggcag tgatacctgc ttctttcaaa tgttctacaa tttcttcttg cgtgccgatt
     4921 tgataatctg tcaccaatct tctaattttt tcaagtctct cttttttatt catttttaaa
     4981 ttgactatgc gccctctcta ctgcttcttt aatctcagca aaaatctgat tgcttgctga
     5041 cttttctttt ttcaaatacg ctaaaaattc aatatttcca tgtccacctt ggatgggaga
     5101 aaagtccaag ccaaggactg aaaaacctgc ctctactgcc atagctgtta cagattcaag
     5161 gacattctga tgaatcttag catctcgaat aattccattt ttcccaatct gctcacgtcc
     5221 tgcctcaaac tgaggtttga caagtgctac cacctgacct tgatcagcca agacacggtg
     5281 caaggctggc aaaatcagac taagggaaat gaaactcaca tcaatactgg caaagctcgg
     5341 ctcctgctcg aaatcagtct tttcagcata gcggaaattg aactgctcca tgctaacaac
     5401 tcgtgggtct tggcgtaatt tccaagccaa ctgattggta ccaacatcga ctgcaaagac
     5461 caacttggca ctattctgta gcatgacatc ggtaaaacct ccagtagagg ccccgatatc
     5521 aatcgtagtc gcgccatcca ccgacaaatc aaagacctgc aaggcctttt ccagtttcaa
     5581 accaccacgg ctgacatact tgagtttctc tcccttgagt tttaattcgg tgtcatctgg
     5641 aattttctct cctggcttgt caaaccgttc tccattaagg actgctacga ctaggccagc
     5701 cattacacct cgcttggcct gctctctcgt ttcaaacaac ccctgtttat aagctagtac
     5761 atccactctt tccttagcca ttgattctca aactttctac tacacttaca atcgattctg
     5821 tttcaaaggg aagctgctgg gcaatttctt ctaatttttc attagcttga tccagggttt
     5881 ggttacaaaa ggcaatggac tcttccaagc ccaacagggc aggataggtt gatttttctg
     5941 cctgcagatc cttttgaggt gtcttgccga tttcctcaaa actagctgtc acatccagta
     6001 catcatctct gacttgaaaa gcaagtccaa tcaattcacc cacagttttc agcttcacct
     6061 gcatttcagg tgacaattca gctataatag ctgccgcttg gaagggatag gctagtaact
     6121 tcccagtctt attggcatga ataatctgaa gttcttccaa agacaagtgc tggtgttcgc
     6181 cctccatatc caaaacttgc cctgccacca tacccagact acctgaagca agggataagt
     6241 tggcaatcaa gtccacctta atctgacttg gcaaatctgc ctgcgcaatc aaggcatatg
     6301 ggtctaagaa taaggcatct ccagccaaaa tggccatagc ttcaccgaat ttcttgtgat
     6361 tggttaaccg ccctcttcga taatcgtcat catccatagc aggaaggtca tcgtgaatca
     6421 agctccctgt atgaatcatc tccaaggcag tagctacctg cgcgtgagca ggtttgatgg
     6481 taacctgcaa ggcttccaga acttctaaca agagaaaagg ccgaatacgc ttgccaccag
     6541 catgaataga atagagaaca gactcccgta aactagaggc aaactgctgg tctccataaa
     6601 aatcttccaa agccgactcg acaagagcta atttttcttg ctttttcatt caaaatcact
     6661 ttctgttccg tcttcttgca tgaccttgac caaggtcttt tcagccttgt ccagcgtagc
     6721 ttggagctct tttgacaaga ccatgccctt ttgaaaggca gtaatcgcat cttccagagc
     6781 aatttcacca ttttccaaac tttggacaat ggtttccagt tctgctagat tttcctcaaa
     6841 tttcttttgt tttgacatct ttaacctcta attctacttg accatctcgc atcaaaagcg
     6901 ttacttggtc ttttttcttc aaactctcaa ccgaatctac aatggactct tcttttttga
     6961 caatagcata accacgcgcc acgattcggc tagtatccaa catgagcaaa gcttccgaaa
     7021 gtcgcttggc ctcagcaacc ttggcgtcat aaactaacgc catttggcta cctaagagct
     7081 tgtccaactg tcctaaacgg tcttgatagc gttggatttt ggtaacaggt gataattgta
     7141 ctaattgatg agttcttgct tgaactaatt gtttgttatc agaaatccga gttcgcaaac
     7201 tttgtttcaa acgcagttgc agttggtcca agcgttgcaa ataaccgtca tacaagcgct
     7261 caggttgtct aaagataaca gactgactgc attttttcaa agcctcttgt ttcttagata
     7321 gaacatttcg gactgccgtt gccatccgtt tttcctgatt ttgcaaatga gttaatacat
     7381 ccaacttggt cacaggtgtt gccagttcag ccgccgctgt tggcgttgca gcgcgtcgat
     7441 ctgccacaaa atctgccaag gtcacatccg tctcatgccc cacactagag ataactggca
     7501 aacgagattc aaaaatagct cgtaccacaa tttcttcgtt aaaggcccag agatcctcaa
     7561 tagaaccacc tccacgacca ataatgagca aatccaaatc gtcccgttga ttagcacgcg
     7621 caatatttct agcaatttcc tccgcagccc cttcaccttg aaccttggta ggataaagaa
     7681 ggatgtcaac acctgggaat cgcctgctga cggtcgtgat aatatctcga ataacggctc
     7741 cactacggct ggttactacc ccaattctct tagaaaattg gggtagagct tgcttgaagc
     7801 gttcttgaaa caggcctttt tctgtcaatt ttttcttaag ttgttcaaac tgaatcgcaa
     7861 gcgccccaac cccatcaggc tcagcttttt caatgatgat ggagtagcta ccacttggtt
     7921 catagacctg tacacgccca atcacattga tcttcattcc ttcttccagg tcaaacccta
     7981 atttctgata aatcccagac cagatggtcg cttgaataac tgcatggtca tcctttaggg
     8041 agaaatattg gtgagtaggt cgtttacgaa agttggaaac ttgaccagtt aaatagaccc
     8101 gttccaagta tgggtcttta tcgaatttca ttttcagata cttggtcaaa gttgttaccg
     8161 ataaatactt ttccatctcc acctactatt catttacttg ctctttcatg ggtattatta
     8221 taccaaaaat atgcctaaaa atctccattt atgtaccatt atgagggaaa aatagaaaaa
     8281 ggaggcaagg cctccacatg tgattatttg ctgtttcgag cttcttccaa aatctttgca
     8341 atcttggtcg tcaacaggtc gatagccacg gtattgctaa ccccttcagg aatgacgata
     8401 tcagcataac gcttagttga ctcgataaac tggtggtaca ttggtttgac cacacctaag
     8461 tactggttaa taacgctatc aaggctacgg ccacgctcct ccatatcacg cttgatacga
     8521 cgaataatgc gcacatcgtc atccgtatcc acaaaaatct tgatatccat caaatcgcgc
     8581 agacgcttgt cctccaagac caaaatgccc tcaacgataa agacatcttg aggctcctga
     8641 cgataggtct tgctactccg tgtatgctct gtatagtcgt aggtcgggat gtccaccgga
     8701 cgccctgcca acaattcctt aatctgctcg atcatcaagt ctgtatcaaa ggcaaaagga
     8761 tggtcatagt tggttttgac acgctcttca aaggtcaaat gagactgatc cttgtagtat
     8821 gaatcatgct caatcatgga aatcttttca tcaggaaaat gcgataaaat ggctcttgaa
     8881 acactagttt taccaccacc agaaccacct gtcactccaa taatgattgg tctattttgc
     8941 atgctatcct ccgttatttt atccgtcgtc tattctatca cattttttac aaaatttata
     9001 gtctcatcag caaaaaacac cagagtcttc cctctgatgt tcgacagttt taacgaaagc
     9061 cctgtagggt ttgagcaagc atctgcaaga aagccagtct ccctctttcc tggctaggag
     9121 ctttggcaat ccataaacca aaatcaatga cagacttagg tacctggtaa tgatgcgtca
     9181 tgaggtaaag tgacaaccct tgactcaaat tggccatgac cgtttggtat ttttcatttc
     9241 tgtctaaatt ttgtatagca agacgaaaca ggtgactcaa ctcactattt tcatttattt
     9301 ctttttgttg aagaagttgc tccacccttt ctcgaaactc atttcgcatc atggaattat
     9361 cacaccaaat cctgggccta agttcccacc agaacctgta atggactcta aatatttatc
     9421 tgaaagaatt ttaaattgct tcatttcaca cctcta
//
LOCUS       DAVMGZ010000077         9311 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000077 DAVMGZ010000000
VERSION     DAVMGZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.078"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            253..1062
                     /locus_tag="VYQ93_001434"
     CDS             253..1062
                     /locus_tag="VYQ93_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3336105.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(1243..2118)
                     /gene="aguB"
                     /locus_tag="VYQ93_001435"
     CDS             complement(1243..2118)
                     /gene="aguB"
                     /locus_tag="VYQ93_001435"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV3336106.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(2128..3213)
                     /gene="aguA"
                     /locus_tag="VYQ93_001436"
     CDS             complement(2128..3213)
                     /gene="aguA"
                     /locus_tag="VYQ93_001436"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV3336107.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPVVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVAIDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(3210..4337)
                     /gene="nspC"
                     /locus_tag="VYQ93_001437"
     CDS             complement(3210..4337)
                     /gene="nspC"
                     /locus_tag="VYQ93_001437"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV3336108.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(4337..5596)
                     /locus_tag="VYQ93_001438"
     CDS             complement(4337..5596)
                     /locus_tag="VYQ93_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV3336109.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(5593..6453)
                     /gene="speE"
                     /locus_tag="VYQ93_001439"
     CDS             complement(5593..6453)
                     /gene="speE"
                     /locus_tag="VYQ93_001439"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV3336110.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            6834..7424
                     /locus_tag="VYQ93_001440"
     CDS             6834..7424
                     /locus_tag="VYQ93_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3336111.1"
                     /translation="MSFRQLNQDCLIALEDTGHYAFNLLNFLHEQGYKVYTYTPLLIK
                     EFAKSLSLRKTKTNKKDAHGIALKLLSDPNREQFQHDNRQVELKILTRHIHRLKKKQS
                     DWKVQYTRCLDIIFPELDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTAS
                     NSRAFCRALKSSPNHQIEIRNSLDKTIDFSYSLSFS"
     gene            complement(7390..8865)
                     /locus_tag="VYQ93_001441"
     CDS             complement(7390..8865)
                     /locus_tag="VYQ93_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV3336112.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSARKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         2704 a         2079 c         1789 g         2739 t
ORIGIN      
        1 tcttcagatt agttgagtaa tctgatactc atacctactt acaaaaaaac tattatatta
       61 agttggtttt ttaatttgtc tagttgcaac attgacaaac atagtatagc atatctttgc
      121 caaatatcct cttcaaatca tgaattgtca tcaaaacatc ttaaactata aaatcaatta
      181 gtctcaagct ttctatcaat ttcttctcca aaatatgcta taatactacc aaaagataaa
      241 gaaggaagac ctatgattaa actactagcc ttggatatgg atggaaccct cctcaatgaa
      301 gccaaggaaa tcccacaagc tcacattact gctattcaca aagccattga aaaaggtgtc
      361 aaactggttc tctgtacggg tcgccccctt ttcggtgtcc tcccctacta caaaaaactg
      421 gggcttgacc tccagaacga atatgttatt gttaacaacg gttgttcaac tcaccaaacc
      481 agtgattggg gtctagttga ctggaaagaa ctgagtccag ctgacatcga atacctctat
      541 gaccttgctg aaaagagtga tgttcagttg acactttttg acgagtcaca ttattttgtt
      601 ctcggtggca agcccaatca agttattgaa aatgatgcta aactagtatt ttcagacctg
      661 actgaaattt ctcttgagga agcgactagt ggaaagttcc ggatgttcca aggtatgttt
      721 ttaggaacaa aagaacaaac agacgatttt gagcagcgtt ttgctgaaga gctttgccaa
      781 cgattcagtg gagttcgttc gcagcctgtc atttatgaag caatgccact tggaacgaca
      841 aaggctactg ctctttcacg actagctgag attttgaaga ttgattcctc agagattatg
      901 gccatgggcg atgctaataa cgatatcgaa atgctccagt ttgcagggct tgggattgca
      961 atgggaaatg ccagcgatta tgtcaaatct cttgcggatg ccgttacctc aagcaacgaa
     1021 gaagacggcg ttgcgcgtgc tattgagaaa tatattctat aaaaaagaaa agcaaataga
     1081 cagaagttac tgctatttgc ttttttgcta atattttaaa atacggacta atcaatagac
     1141 aagaatagcg aatcataaaa atcgatttac tagatgccat agtatcttat agttgctaat
     1201 aagaagttag tctagcagaa tgaatctctc atttctccca cactaatctg taatttgtct
     1261 atacatttct ggtcttctat ctcgaaacaa gccccagttt aggcgttcac ttgctccctt
     1321 gtctaggtca taagtagcta acagaacagc ttcttcttgt ctttcagctc gttctagaat
     1381 agctcctgtt tcatccgtca taaaggagga accgtagaag tcaagactgg aactctgtcc
     1441 gccattttcc tcactaggag taacctcctc taaaccataa cgattggctg cgatgactgg
     1501 aacaatattc gctgctgcgt gcccttgcat agtacgttgc cagtgaccac aactatctgt
     1561 atccaaaatt ggctctgaac cgatagctgt aggataaaag agcaattcag caccattcaa
     1621 tgcaagacag cgcgctgttt cagggaacca ttgatcccaa cagataccga taccaatctt
     1681 agcatagcga gtattccaga ccttgaaacc agtgttacca ggcgtgaaat agaatttttc
     1741 ttgataataa tggtcatctg gtatgtgggt ctttcgataa acgcccagca cttccccatc
     1801 tgcatcaatg acggcaatag agttatacaa gacattacca tctttttcat agaaactgat
     1861 tggtaaaaca acttgtagtt ccttagcaat caccttaaaa tgctgaatgg cagtattttc
     1921 cgctacagat tgggcatact ggtagtagtc atactgacgt tcctgacaga aatagggatg
     1981 ttcaaacaac tcgggcaaga gaataatttg ggctccttgc tcagcagcct gacgtactaa
     2041 acgctctgcg gtttggatat ttgttgccac atccttagcg cattgcatct gaatggttgc
     2101 aactcttaca tttctcatct ttttctccta ttctggaatt tgttgggtga tacagtggat
     2161 attaccacca cctaagagaa tatctctggc tggtattccg acaactttac ggtctgggaa
     2221 acacttgctg aggatatcta tggccacttg gtcgtttaca tcctcaaact gtggaaccaa
     2281 gacagccttg ttggcgatat aaaagtttac gtaggaagct gctagtcgtt cacctgcgta
     2341 tcgctcttct tctccttctt catagatgta gcctggcaaa tcttcttctg tcacaacttg
     2401 tcgaactaca gggataggca atttatgaat ggtgaagtga caaccttttg catctgtttc
     2461 ctgttctaag agttcgagat ctgcttttga catggcatac tggggatcat tttcgtcatc
     2521 tgtccaagcc aaaacaagct cagcaggacc aacaaaggca gcaacattat cgacgtgttc
     2581 attggtttca tcctgataaa taccataagg aagccaaata actttttcag caccaagact
     2641 ttctaataat gtgttttcaa tctcctcttt agtcaagtta ggattgcgac caggactaag
     2701 caagcaactt tcagttacga gaatagttcc ttgaccatcg ctatggattg cgcctccttc
     2761 cagtacaaaa ggtttagcat catagacagg cctttccaaa gcctcagcaa aacgactggc
     2821 tacttggtca tcctcttcat aatcttgata aagaccatca taggtgcctc cccaagcatt
     2881 gaaggcccaa tccacggcta atttcttacc tttatcattg acgagaatgg ttgggccagt
     2941 atcacgcgcc caggcatcat tggtgggaat gtctaaataa acaaccttgt ctccaagata
     3001 ggattgggct tcagatagat aggcctgctc caccaaaaga tagactcttt ccccttctgc
     3061 tatggtctcg atgatctgag taaatgctct tttagcagcc tttccttgaa aaggccatga
     3121 tcctggtcga gtcggccata tcatgagggt accatgatgg ggttcgtact ctgctggcat
     3181 gtgatagcct aatttttttg gactgtccat catgataatc tccctttaaa gtcttgatag
     3241 ccaaaagctt tgagtaagct acagtctccc tgttcgtcca tgagatagag acttggcaat
     3301 ccaataccat taaaggtatt atttttgaca aaagaataaa tggccatgtc ttgaaaataa
     3361 agtctgtctc cgatttggac tggattttca aaactataat caccaatcac atcgccagtc
     3421 agacaggtat tagaagaaag tctgtaggta tgggcttttt cctgtgcctc aaagccattt
     3481 ctcaaaggtg gacgataggg catctcaagt acatcaggca tatggcaggt cgcagaggcg
     3541 tctaaaacca agatttccat accgttttct acaatatcta atacctcagt tgctaaataa
     3601 cccgcattaa gcgcaatggc ttcaccaggc tcgatataga tttcaagatt gtaagttttt
     3661 cggatacgct tgatttctga aatcagcaaa tccacatcgt aaccttctct tgtaatatga
     3721 tgaccaccac ccatattgag ccattttacc tcatgtaagt agggaccaaa ttgttcttct
     3781 actgctttca aagttgtttg taaatcatct gctccctgct cgcaaagggt atgaaaatga
     3841 agtccgtcaa ccaaatctag caaatcactc ggaatcttgt ctatagtaac tccaaagcga
     3901 gaacctggtg cacaagggtc atagagcgcg tgatctcctt gagttgaaca ctgagggttg
     3961 aggcgcaaac caacactgac accagcctct cgacaacgag ccccatgctt acgcaactgt
     4021 ctctctgagt taaagacgat atggtctgtt atcccaagca attcctccaa gtctgcatcc
     4081 ttgaaagcag gcgcaaatac atggacttca ccaggaaatt cttcccttgc caatttggcc
     4141 tcatagagtc cactagctgt cgtacctgat agatactggc taatcaaggg ataagttttg
     4201 tagagggaat atgccttctg ggcaagcaag accttgcaac cggcctcttc ttgtacatat
     4261 tgtagaatgc ggcaattagc ttctaacttg gccaagtcaa taacataggc tggtgttggt
     4321 acttgttcta acttcattag tccaccattt gtggattttc aaccacaacc catggcaaac
     4381 catactcatt caaagcttcc atgaatggat ctggatctaa ctcctcaagg ttatacactc
     4441 cagcttgttt ccaagttccg ttcatgacta attttgtccc aatcatggct ggaactcctg
     4501 tcgtgtatga aattgcttgt gacccgactt ctgcatagca ctcttgatgg tcgcaaacat
     4561 tgtagatgta gatagtcttt tcaacgccat ctttgacacc tgtaaagata catccaatat
     4621 tggttttacc tacagtacgt gggccaaggc tagcaggatc tggaagcaag gctttcaaaa
     4681 attgaattgg aacaatttct tggccgttaa agttaatggt atccgtacga aggagtccaa
     4741 cattttcaag acatttcatg tgcgtcaagt aagattgacc aaaagtcata aagaaacgaa
     4801 tgcgtttgac acctggaatg ttcttggcca atgattcgat ttcttcatgg tgaaggagat
     4861 acatatcttt ttgtccaact tgagggaaat catactcacg cttgatagac atagcttcga
     4921 cttcgaccca tttcccatct tcccagtaag aacctggcgc agaaacctca cggagattaa
     4981 tttctggatt aaagttggtt gcaaatggat aaccgtggtc accgccatta cagtctaaaa
     5041 tgtcgatata atggatttca tcaaaatagt gtttgagggc ataagctgaa aagacactag
     5101 ttacacctgg gtcaaaacca gaaccaagaa gagcagtcaa gcctgcttct ttgaatttct
     5161 cttgataagc ccactgccat gagtagtcaa agtaggctgt aaaaccaagt tccttacaac
     5221 gtttttcgta gatagcacgc cactcagggt cttctgtgtc ttctgcttcg tagttggctg
     5281 tatcgatata gtgaacacct gttgccaaac aagcatccat aatggttaaa tcttgataag
     5341 gcagagctac attcaaaaca gcttctggtt tgtagctttc aatcagggca atcacttctt
     5401 caaccttgtc agcatcaagt gctgcagttt caattttagt acttgttttg ccttctagct
     5461 tcgctttcaa gtcatcgcat tttgacttgg tacggctagc aatcataatc tctgtaaatg
     5521 tttcgctatc ttgacaaatc tttgaaatag caacttgggc aacgccccca caaccaataa
     5581 ctagtaaacg actcattttt ttccttcctc ttcttctaaa atgtcctcaa catacttggg
     5641 caacataaag gatcccacgt gtaagtttgc agtgtagtat tctgtgaaaa gctggcgttt
     5701 tttccaacct tccttgtcaa aatctttgac agggtggtat tttttcgatg caaatccaaa
     5761 caaccaatag ccagctgggc tagttggaat atgggcctga taaacccgac tgattggaaa
     5821 ggcttgattg accttgcggt gcatgcttcg gcaggccgac tcatcctcgt caaagaaggg
     5881 actcccatgc tggtaaatca tgatgccgtc ttccttcaga gctcgataac tattgccgta
     5941 gaattccttg gtaaagagtc cttccgtatg gccaaatgga tctgtcgcat cgttgatgat
     6001 aatatcgtaa tcatcttcgc agtttcgcaa aaagcgtagc ccattttggt agtaaatggt
     6061 aacacgagga tcatctagcc ctgcagcaaa gtctgggaaa tactcacgac agacctcgac
     6121 caacatctca tccggttcca cgatatcgat ttgttccagt tcaggataga gtgttaatac
     6181 ttgggcaaca ccgccgtcac cacccccaat aaccaatact ttctttggat ttgggtggac
     6241 agccatggga acgtggacgg tcatttcatt gtagacgaaa tcatccgcat ctgagaacaa
     6301 gacatggcca tttaaaatca gtattttccc aaaagctggc gtatccaaga cttcgatatc
     6361 ctgccattca ctttttccag cgtaaagttg cttggctgtt ctcagagaca atttgacatc
     6421 tggagtatga acttcagaaa accataaatc catctatttc tccttatatt atatttagcg
     6481 cagttagcta ctgccaaagc ccaagtggta tacttggaat aagtcactgt ggattagttc
     6541 atttcctcca atcgcttgac gatttccgaa aggctctaac cacagctttt tgtttcaatt
     6601 ggtaatgagt ttgataacgc atagacgttt tatctcgtct aaggtaacaa ggcaaaaagt
     6661 tcgtggataa ccaagtagac ttgaaatact atatctggag gtactcccat gctttatgta
     6721 ggtattgatg tcgctaaaaa taaacacgat gttacagcct tgaatgttcg aggaaaactg
     6781 ttcttaaacc actcactttt tcaaataata aagctggttt tgaactctta gatctgtctt
     6841 ttcgacagct caaccaagac tgtctcatcg ctcttgaaga tactggacat tatgccttta
     6901 atctactgaa cttcctgcat gaacaaggat ataaagttta cacctacact cctttgctca
     6961 tcaaggaatt tgccaaatca ttatcacttc gcaagactaa aacgaataag aaagacgctc
     7021 atggcatcgc tcttaaactt ctttctgacc ccaatcgtga acaatttcaa cacgataatc
     7081 ggcaagtaga actaaaaata ctgactagac atattcatcg tctcaagaaa aaacagtctg
     7141 attggaaagt acaatacact cgttgtcttg atatcatctt tcctgagttg gataaaatcg
     7201 ttggaaagca ttcagaatat acctaccaac tcttgacacg ctaccctaat cctcagaaaa
     7261 ggattgaggc aggatttgat aagctgatag aaattaagcg attgaccgct tctaattcaa
     7321 gggctttttg ccgtgctcta aaatcatcgc caaatcatca aattgaaata agaaatagtt
     7381 tagataaaac tattgacttt tcttatagtt tgtctttctc ttaataacgt tgatatgatt
     7441 gacctctgga tcttccgtcc cttggaggga gcaaccacgt tccttggcga attggatata
     7501 gtcgacaatt tctcgtgtaa tgcgttcacc aggagccaag ataggaatcc ctggaggata
     7561 gcacatgaca aattctccac agacctgtcc aacagaatca tccaaagtta aactttttct
     7621 tgctgaatag aaggcttctt gcggagacag cactaactcg ggctgaatat attctcctgc
     7681 tatcaaatct tttccatctc ttgaatagag tctcttaata tcagccagag caccaaccaa
     7741 gcgctcgatg tcttggatgc ggtcgccgat agaaatatag gccaagatat tgccgatatc
     7801 accaaactcg atctgaatgt cgtattcgtc tcgcaagagg tcataaacct cgatacctgt
     7861 taagccaata ccctgagtgt aaactgacag cttagttacg tcaaaatcgc aaaccgaaac
     7921 accgtctatt aattcttttg agtaggcata gtagccaccg atagcattga tttcacggcg
     7981 ggcatactca gatagctcaa tgactttctc aaacgactct ttaccacgaa gggccaagtt
     8041 gcgacgtgaa atatccaaac tagccatcaa caagtaagag gcagatgtag actgggtcag
     8101 gtttataatc tgacgaacgt actcgggatt catctgctcc ccgattaaaa gaagcgaact
     8161 ttgtgtcaaa ctcccaccag acttatgcat ggaaactgct gccatatcag ctcctgcatc
     8221 catagcagaa attggaagtt taccagtgaa atgcaaatgc gctccatggg cttcatctac
     8281 taaaaccatc atgccagctt catgagccat ttctgtcaac ccctttaggt ctgaacagat
     8341 gccgtagtaa gtaggattgt tgattaggat agccttagca tctggatggt cctttatggc
     8401 ctgtgctact cggtcatttt caagacctaa agcgatacca atcttaggat ctacactcat
     8461 ctcgatatag atgggaatgg caccacatag aaccaacgca ttgatagcag atttatggac
     8521 atttcgtggc agaataatct tatctcctgc cttgcaggtt gccagaatca tagtctgcac
     8581 cgatgaagtt gttccaccaa tcattagaaa ggcatggcta gctccaaaag catctgcagc
     8641 cagctcctct gcatcacgaa taatcgaaat aggatggcct aaattatcca agggtttcat
     8701 cgaattgaca tcaatgccta cacatttttc tcctaagagt tcgacaagtt ctggatttcc
     8761 ccgtccacgc ttgtgacctg gaacatcaaa gggaacaatc cttttcttgc gtaacttcac
     8821 caaggcctca taaattgggg cttggttttg atctaactct ttcaaaacat actcctttca
     8881 atatttttag gaccttatga aaactaagga actaaaggtt gactcatgaa aaaagagcag
     8941 gttttcacct gctctgcaat cttctgacgt gtttattttg gtttctgttg agtatgtctg
     9001 ttcccgatgt ttcctaactc tctagtagca aatattacgt actttattga tcgtttcaca
     9061 agatgacgtg tgctttcacc acactaaaaa tttatgaatt aggacaagtg tcctaacatc
     9121 aacttataaa agtaggcttt taaccctatc aataatgtgg cttttcaacc acgcttcggc
     9181 attcaaccct gcctgtccgt aggcattttt tactcgggtt tatatttgct agaaaacatc
     9241 atctcatttt ctaagcttta ttactatatc atgtttttaa gaacttgtaa agtatttttt
     9301 gataaaaaac a
//
LOCUS       DAVMGZ010000078         9286 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000078 DAVMGZ010000000
VERSION     DAVMGZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.079"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            47..1210
                     /locus_tag="VYQ93_001442"
     CDS             47..1210
                     /locus_tag="VYQ93_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV3336113.1"
                     /translation="MAAKHAIAVEANTGKILYEKDATQPVEIASITKLITVYLVYEAL
                     ENGSITLSTPVDISDYPYQLTTNSEASNIPMEARNYTVEELLEATLVSSANSAAIALA
                     EKIAGSEKDFVDMMRAKLLEWGIQDATVVNTTGLNNETLGDNIYPGSKKDEENKLSAY
                     DVAIVARNLIKKYPQVLEITKKPSSTFAGMTITSTNYMLEGMPAYRGGFDGLKTGTTD
                     KAGESFVGTTVEKGMRVITVVLNADHQDNNPYARFTATSSLMDYISSTFTLRKIVQQG
                     DAYQDSKAPVQDGKEDTVTAVAPEDIYLIERVGNQSSQSVQFTPDSKAIPAPLEAGTV
                     VGHLTYEDKNLIGQGYITTERPSFEMVADKKIEKAFFLKVWWNQFVRFVNEKL"
     gene            complement(1441..2853)
                     /gene="sufB"
                     /locus_tag="VYQ93_001443"
     CDS             complement(1441..2853)
                     /gene="sufB"
                     /locus_tag="VYQ93_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV3336114.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(2907..3347)
                     /locus_tag="VYQ93_001444"
     CDS             complement(2907..3347)
                     /locus_tag="VYQ93_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV3336115.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQTSLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            complement(3334..4560)
                     /locus_tag="VYQ93_001445"
     CDS             complement(3334..4560)
                     /locus_tag="VYQ93_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV3336116.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            complement(4571..5833)
                     /gene="sufD"
                     /locus_tag="VYQ93_001446"
     CDS             complement(4571..5833)
                     /gene="sufD"
                     /locus_tag="VYQ93_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV3336117.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGDDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            complement(5861..6631)
                     /gene="sufC"
                     /locus_tag="VYQ93_001447"
     CDS             complement(5861..6631)
                     /gene="sufC"
                     /locus_tag="VYQ93_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV3336118.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            complement(6781..6975)
                     /locus_tag="VYQ93_001448"
     CDS             complement(6781..6975)
                     /locus_tag="VYQ93_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV3336119.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            complement(7003..8658)
                     /gene="dnaX"
                     /locus_tag="VYQ93_001449"
     CDS             complement(7003..8658)
                     /gene="dnaX"
                     /locus_tag="VYQ93_001449"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV3336120.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            complement(8658..9155)
                     /locus_tag="VYQ93_001450"
     CDS             complement(8658..9155)
                     /locus_tag="VYQ93_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV3336121.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
BASE COUNT         2708 a         2078 c         1754 g         2746 t
ORIGIN      
        1 ctctctttta gggggtgctt ctactgctgt tgctcaagat tttaccattg ccgctaaaca
       61 tgcgattgct gttgaggcaa atactggtaa aattctctat gagaaggatg caacgcaacc
      121 tgtcgaaatt gcttccataa caaaattgat tactgtttat ctggtctatg aagctttgga
      181 aaacggaagt attaccctct ccactcctgt agatatttct gattatcctt atcaattgac
      241 gacaaattct gaagccagta atattcctat ggaggcccgt aattatactg tcgaagagtt
      301 gcttgaagca actctggtat ctagtgccaa cagcgccgct attgccctag ctgagaaaat
      361 tgctggctca gaaaaagatt tcgtcgatat gatgcgggca aaactcttgg aatggggaat
      421 tcaggatgcc actgttgtca atacgacagg tcttaacaat gaaactctag gggataacat
      481 ttacccaggt tctaaaaaag atgaggaaaa taagcttagt gcttatgatg tcgctatcgt
      541 tgctcgcaac ctcatcaaaa aatacccaca agtcttagaa atcaccaaaa aaccttcttc
      601 tacttttgct gggatgacaa tcacttcaac caactacatg ttagaaggta tgcctgctta
      661 ccgtggtggt tttgatgggc taaaaacagg aacaacagat aaggctggag agtcttttgt
      721 tggtactact gtcgaaaaag gcatgagagt catcacagtt gttttaaatg cagatcatca
      781 agacaataat ccttacgctc gatttacagc tacatcttcc ctaatggatt atatttcttc
      841 tacatttaca cttcgcaaaa tcgttcaaca aggcgatgcc tatcaagata gcaaagcccc
      901 tgtacaagat ggaaaagaag atacagtaac tgcagtggct ccagaggata tctatctaat
      961 agaacgtgtt gggaatcaat cttcccaatc tgttcaattc acacctgatt ccaaagcaat
     1021 cccagcacca cttgaagctg gaacagtggt tggccatttg acttatgaag acaagaactt
     1081 gattggtcaa ggttacatca ccacagagcg ccctagtttc gaaatggtag cagacaagaa
     1141 aattgaaaaa gccttcttct taaaagtttg gtggaatcag tttgtccgct ttgttaacga
     1201 gaaattataa aaaatcgcga ttttaaaccg cgattttttg atacaagttc atcttcaaaa
     1261 tccttgattt tgagtaaatc taatactcaa aatcaaagaa taaactagga agctagccgc
     1321 aggttgctca aaacactgtt ttgaggttgt agatgaaact gacgaagtca gtaaccatac
     1381 atacggtaag gcgatgctga cgtggtttga agagattttc gaagagtata aaatcaaatt
     1441 ttatccaact gatccctcca tttcatagct aatcaagcgg ttcagctcaa ctgcgtattc
     1501 cattggaagt tcttttgtaa agggttctac aaatcccatg acaatcattt cagttgcctc
     1561 agattctgac aatccacggc tcatgagata atacaattgc tcttctgaaa tcttagatac
     1621 tttggcttcg tgttccaaag ccacttgcga gttgtgaatt tcattaaatg gaatagtatc
     1681 tgatgctgac aagtcatcca tgataatggt atcacattca atgtgggaaa cagatttctt
     1741 agagttcttg ttaaaggtga cttgtccacg gtagtcaacc tttcctccac ctttagcgat
     1801 ggatttagac acaatagacg agctggtatg tggagcattg tgaatcatct tagcacccgt
     1861 gtcttggtgt tgccctgcat tagcaaaggc gatagagagc atggtaccac gcgctccttc
     1921 tccatcaagg taaacagatg gatatttcat agtcgttttg gcacccaagt ttccatcaat
     1981 ccactcaaca gtggcatcct tttgagcctt agcacgcttt gttaccaagt tatagacgtt
     2041 atcagaccag ttttggatag ttgtataacg catataagct ccgtccaaag caaaaatttc
     2101 tacaatggca gcgtgtaagc tattgcttga atatgttggt gctgtacatc cttctacgta
     2161 gtggacgctt gctccctcat caacgataat caaggtacgt tcgaactgac ctatattttc
     2221 gttattgata cggaaataag tttgaagtgg aatatctacc ttgacacctt ttggcacgta
     2281 gataaaagtt ccacccgacc atactgctga gttgagggct gccaacttgt tatctgtcgg
     2341 cggtaccaac ttcgcaaagt attgtttaaa taagtctggg tattccttga gtgcggaatc
     2401 tgtatctgta aagataatac ctaatttttg gaactcttcc ttcatgttgt ggtaaaccac
     2461 ttctgactcg tactgggcag aagcccctgc taaataagca cgttcagctt ctggaatccc
     2521 gatacgttca aaggtttctt taatcttttc aggtacatca tcccaagaac gggctggttt
     2581 gtcagatggt ttttggtagt agattaagtc atcaaagtca atctctgaca agtctgctcc
     2641 ccaagtttgc atgggcattt ttttgaaggt ttcataagac ttcaaacgga actccaacat
     2701 ccactcaggc tcacccttag cagcagataa ttcacgaata acaccttcgt tgagtccttt
     2761 tcctgtcgat aagacaggct ctacatcgtc atggaaacca aatttatatt caccaaggtc
     2821 aattggtttt ggttctactc tttcttcagc catagtatcc tttctttctt catttctaat
     2881 aattcataag acaaaagaaa cttgtcttac tgtttttctt gattttcaat tgttttctta
     2941 agggcattcc aagctagggt tgcacacttg attctttgag ggaatttggc aacacctgac
     3001 aagaatgccg cgtctccaag ttggccttga cgctcatctt tttgcccctg aaccatttca
     3061 gaaaaaatag tcgccagttc taaaatttct tgtttggttt ttcctaaaac ggcatctgtc
     3121 atcatactag cagaagcagt tgaaatcgtg catcctgaat ttagaaaagc aatatcttcc
     3181 aaacggtcct ctgcatcaaa cttgacagag aggttgatga catccccaca agtcggattg
     3241 ttgagactgg tttgctcagc atcttctaac ttcccttggt gatgtggatt tttcgaatgg
     3301 tctgctacca ctgccatata aaggctatct agtttagaaa gtgccattga aaaactcctt
     3361 tgtcttttgt agggcatcga ctagtttgtc gcaatctgcc ttggtattgt agatataaaa
     3421 acttgcacga gctgttgctg ggacttccaa atactgaagc aagggttgcg cacaatggtg
     3481 accagcacga acagccactc cttcataatc cagagccgtc gcaagatcgt gaggatggag
     3541 atcacctagg ttaaaggcaa taacacccga acgttgagcc aaatcctgag aaccgtaaat
     3601 ggtcaatccc tcaattgcct gcagttttgg atagacgtac gcaatcaatt cctgttcatg
     3661 agcttcaacg gcatccatac caatcttttc cagataatca actgcagtcg caagtccaat
     3721 agctcctgcc atatttggcg ttccagcctc aaatttccaa ggcaattcct tccaactagc
     3781 aaattgctcg tagacaaaat caatcatctc gccgccaaat tctactggag acatttgctc
     3841 aagatacttt tctttgccgt aaaggacacc gataccagtc ggaccagcca tcttgtgacc
     3901 cgaaaaggcg aaaaagtcca gatccaagtc ctggacatca atcttcatat gaggtgtaga
     3961 ttgagcacca tccactacca taattgcccc aacttggtgg gctaattgag tgatttcctt
     4021 gatcggattg accacaccaa gaacattgga ggcatgagct agggaaacaa atttaacctt
     4081 atcagtcaat ttagctcgca aatcctccat atccaaggca ccgtctttaa gatagacata
     4141 gacaagctct gctccagtct ttcgacaagc ttcctgccat ggaatgatat tagaatggtg
     4201 ttccattact gaaatcaaga cctggtctcc ctcagcgaga atttcctcag caaagcgtgc
     4261 cacccagtta aggctggttg tcgttcctct ggtaaagaga acttcctttg tagagcctgc
     4321 attaataaac ttacgaatgg tttcacgagc agcttcataa gaagctgtcg ctcgttccgc
     4381 taaggtatgg acaccacggt gaacattggc attgtcctgc tcatagtagc tgttaatagc
     4441 tttcagaact actagtggtt tttgtgtcgt cgcagcattg tccagataga ccagaggttc
     4501 atcattgaca atctgatcta aaattggaaa atccttgcga atcgcttcta catctaacat
     4561 aggcttcccc ttagcgtttt gacaatttct cttcgatagt tgcaatcatt tcatcacgaa
     4621 cttccttgac tggaatctcc acgataacag atccaaggaa accacgaaca accaaacgct
     4681 ctgcagttgc cttatccaag ccacgactca tgaggtaata catgtcttct ggatctacct
     4741 gaccaataga ggctgcatgt cctgcagtta cgtcattttc atcaatcaaa agaattgggt
     4801 tagcatctga acgcgcttgg tctgaaagca taagaacacg gctctcttgt tgcgcatctg
     4861 ctcccttagc acccttgatg atgtggccga taccattgaa agtcaaagtt gctttttcaa
     4921 ggataacccc atgttgtagg atatttccga tagagttgca gccatagtta gttacacgag
     4981 tatcaatccc ttgtacctga cgaccacttg aaagagctac aaccttcagg tcagcatggc
     5041 taccattacc aatcaagtca ctatcaaaat cagcaacgac atttccttcg ttcatgacac
     5101 cgatagccca gtcaatactt gcatcgtcgc ctaatttacc acgacggcta atgtaggcag
     5161 tgacgttttc acctagacgg tcgatagcag caaacttgac ttgcgcacca gaacgtgcaa
     5221 tcacttccac tgtgatattg gcagttgctt tgtcacttcc ttcaccgcgt gactctaaac
     5281 gctccagata actaatctta gaatttttac cagcgataat cataatatgc ttgttaaacg
     5341 gcacattgct atcgctatct tggtagaaaa ttccttcaat tggctctgta atttctacgt
     5401 tatctggaat atagagtaca gcaccactgt taaagtaagc tgtgtggtaa gccgccaact
     5461 tgtcatcatc atacttaaca gatgacatga agaattcttc gatcagctct ggaatttctt
     5521 ctaaagctga gtgaaagtct gtgaagacaa caccctgttc agctaactca actggagttt
     5581 gttcgaaaac agtttgagtt cctacttgca ccaacttcaa gtgatgatct aaagctgtga
     5641 aatctggaac atttgctgat ggctcatttt ctgtaatcgt tccatcaccc agattccaac
     5701 ggtggaattt gacacgctca ataactggta attccaaagt ctcaatcttg tcaaaagctt
     5761 tttgacggag atcagccaac cagcttggtt cagcgtgcat ttctgaaaaa agtttaatat
     5821 tttctctagt catttacttc tcctaaaaga tacgagggaa ttacaattct tccttgtagt
     5881 cgtagccaag ttcttcagct agttttgcgt atccttcacg ttccaaacgc gcagccaatt
     5941 ctggaccacc agaaaggaca acacgacctt ccatcatcac gtgtaccaca tcaggtgtga
     6001 tatagttcaa aagacgttgg tagtgagtaa tgatcatagc accaaaacct tcaccacgca
     6061 tggcattgac acctttagac acaactttaa gagcgtcaat atcaagacct gagtcaatct
     6121 catccaaaag agcaaatgtt ggctccaaca tcaaaagttg aagaatttca ttgcgttttt
     6181 tctcaccacc agagaagcct tcgttgaggt aacgctctgc catttcttct ttcatgttga
     6241 gcaattccat tttttcatct agcttagtaa taaactcacg aactgaaatc ttctcatcat
     6301 cttctttacc cgcattcata gcggcacgaa gaaactcagc attggtaatt ccagggattt
     6361 ctgatgggta ttgcatagca aggaaaagtc ccatacgcgc acgctcatcc acttccaact
     6421 caaggatgtt tacgccatca aacaaaactt cacctttagt tacttcatag tttggatttc
     6481 ccatgatagc ggcagaaaga gtcgatttac ctgtaccatt tggtcccatg atagcggcaa
     6541 tttctcctgt tttcagggtc aggttaaccc cttttaaaat ttcttttcct tcaatctcaa
     6601 catgaagatc tttgatctct aatactgaca tgatagttcc tttctttttc aaaactttta
     6661 cagtacttac tagaaaaacg tctgatttcc ccaaaaatag agcaaaaatt ctataaatat
     6721 acttttctag tataacaaaa aaaagcctaa aaggctttga ttttgtctgg aagctgaggg
     6781 ctagtctttc ctattaaaat gctgatcgat gacatcaacg attttgccca tcacataact
     6841 gactcggaaa tttctaaaga gtaaaattgc ccaaaaggct gccataatat agcggccagt
     6901 catccaggct tcattgaaaa aataggtctc agcagctgta aatagcgcca taataataaa
     6961 ttgttgttta ttcataaact tattgtatca tgaaatcttt ctttagtctt cctctacctt
     7021 cactttatca gccaaaaatt caaatccttc tggaatcagg ctttcttcta cttctttttc
     7081 agtttgagaa gatttggctt tggctgaaaa ggctgcgcga acttctttcc attcctccat
     7141 ggaaatagct aaaatctcag gtgaaaaacc tgccgcctga ctgaggatat taccaaacat
     7201 ggtattgaga ttgtctcgtt tcatagtttg accagcattg aagttagact caaaagcaag
     7261 aatagcatgg tgttcattgg cagcaaccgg ttgagaacca actagcagag ccttgtccgg
     7321 cccacctaga ctttcaatta cctctcccca ggcattctgc aaacgaatta gattttgacg
     7381 tgctaaatca ggattttcga cggcctcttg taagatagat tgcactttat tgcgatcgac
     7441 acgatagact gttttgcccg tagctggtcg actaggagct ggtgcaactt gtttaggaac
     7501 cgcacctgca ttagaaagct cttgtttgag acgggcaact tcctgtctca gcgtagcaat
     7561 ttcattttca accgctcctg atagagctgg ttcgggcttg atttccgcca aacggacggt
     7621 catcatttca gcataaatct tgggctgcaa actagactta atatctgcta aattcactgt
     7681 tgctaagcga atcatttcaa acagattttt ttgaggaagt gccaaatttt ctacaaagac
     7741 tgaactatga tgagtatttt ctccccctgt ttgaacaatt aacaagtctc ttaaatagtg
     7801 caaaagatcg gtcacaaaac gagtcatgct cttaccattg tcaaaaagaa gattcaagca
     7861 agacaaagct ttgggaacat cctgttgaga caaggccgcc acataatcat ccaaggctga
     7921 taggctaatg gtgccagtaa tttcttcaga gatagcagtc gtcagctcat ttccctgtgt
     7981 caaactcagg gcttgatcca aaatagacaa ggcgtcccgc attccacctt ccgcccgtct
     8041 ggcaatgatt tccacagcct ctggttcaga actgatattt tctttttcta agatatagtg
     8101 aatatgttcc ttaatatcct gtgtcttaat tgatttaaac tcaaaacgtt gcacacggga
     8161 tagaatagta gcaggaatct tgtgcaattc agtagtggcc aaaataaaga ctacattctg
     8221 tgttggttct tccagcgtct ttaggagggc attaaaagcc cctgtagaca gcatgtgaac
     8281 ctcatctatg atataaacct tataacgagc aaggctaggc gcataggtag atttatcacg
     8341 aatttcgcga atttcatcta ccccattatt agaagctgca tccatttcaa tgacatcttc
     8401 taaactaccg tccgtcactg cttgacaaat atagcagtta ttgcaaggtt cgccacccac
     8461 ttgattggga cagttcatag ccttggcaaa gattttagca acactggttt ttcccgttcc
     8521 acgaggacca gaaaaaagat aagcgtgact tattttctct tgctccaccg cttgtttaag
     8581 agtcttagcc acaacttctt gaccaactaa ctgggagaag ttttgacttc tatattttcg
     8641 ataaagtgct tgatacatta agatttttcc tcaaacatcg taaagtccca tgctgtcttt
     8701 tcaagcaaaa tagcgacaaa ttgttccaaa taatctcgat ccatagcatc gtaatcctca
     8761 atctctgaag aatccagatc cagaactcca agtaactgac cattcttcat catcggcacc
     8821 acaatttcac ttttagctag actatcacaa gaaatatagt tgagataggt cgtcacatct
     8881 ccaacaataa cagtttcctg aaagtgagct gcctcaccac aaacaccctt gcctagtgca
     8941 atacggatgc aggaaacacc tccttggaag gggcctaaaa ccaattcctt tccatcgaac
     9001 aaataaaagc ctgcaaatac ggtattagga aaacgtgatt ttatgagagc actggcgttg
     9061 gaaagattag ccaaaacatt ggtttcgcct tccaatagga aggacaattc ttcatttaac
     9121 atttgataac gtgattgttt ttctgatttt aacatagaac tattatatca aaaaaggagc
     9181 agagacaaaa gaaaaatccc ttgtttagta aacaaaggat tttgggctct ataatatttg
     9241 tagtgggtaa atcccctatg gatattatgg agcctatttt tgtgta
//
LOCUS       DAVMGZ010000079         9284 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000079 DAVMGZ010000000
VERSION     DAVMGZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9284
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.080"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            454..1497
                     /locus_tag="VYQ93_001451"
     CDS             454..1497
                     /locus_tag="VYQ93_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV3336122.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYYKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1664..3751
                     /gene="topA"
                     /locus_tag="VYQ93_001452"
     CDS             1664..3751
                     /gene="topA"
                     /locus_tag="VYQ93_001452"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV3336123.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3857..4216
                     /locus_tag="VYQ93_001453"
     CDS             3857..4216
                     /locus_tag="VYQ93_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV3336124.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4309..4941
                     /locus_tag="VYQ93_001454"
     CDS             4309..4941
                     /locus_tag="VYQ93_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV3336125.1"
                     /translation="MIYEFCAENMTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            4985..5641
                     /locus_tag="VYQ93_001455"
     CDS             4985..5641
                     /locus_tag="VYQ93_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV3336126.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5660..6292
                     /locus_tag="VYQ93_001456"
                     /pseudo
     CDS             5660..6292
                     /locus_tag="VYQ93_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
     gene            6320..6754
                     /locus_tag="VYQ93_001457"
     CDS             6320..6754
                     /locus_tag="VYQ93_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEV3336127.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTIDAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGCSVSYHDMPSV"
     gene            6766..7803
                     /gene="leuB"
                     /locus_tag="VYQ93_001458"
     CDS             6766..7803
                     /gene="leuB"
                     /locus_tag="VYQ93_001458"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV3336128.1"
                     /translation="MAKKIVALAGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAWPPLPDETLKASREADAILLAAIGSPQYDGAVVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDHILEERNARDINDYSYEE
                     VERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPISMILSVSMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7800..8069
                     /locus_tag="VYQ93_001459"
     CDS             7800..8069
                     /locus_tag="VYQ93_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV3336129.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8073..8432
                     /gene="leuD"
                     /locus_tag="VYQ93_001460"
     CDS             8073..8432
                     /gene="leuD"
                     /locus_tag="VYQ93_001460"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV3336130.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8594..9217
                     /locus_tag="VYQ93_001461"
                     /pseudo
     CDS             8594..9217
                     /locus_tag="VYQ93_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         2871 a         1639 c         2064 g         2710 t
ORIGIN      
        1 tttagtgtaa ttgagaaagg agaaatgatt gtgattgatg ttggctaggt tatgttcaat
       61 gattcctacc gtctcaaatc ttgtcagtaa ggaaaaataa attcttcaaa agtagagatt
      121 acaaggcttg tttaagaaag aattcaaaga ccttgacaaa taaaaataaa atggttatta
      181 taaaaaatgg tctaaaatag atgatgatac ttttcgaaaa tctttttacg tcagctcagc
      241 tttgccttgc tgtgttttga gcaagctacg gttagcttcc gagtttgatt ttcatttact
      301 agaaatgaaa ctgatgagag atatcagtag acatttgagt caggatatta tggaaaatga
      361 taaaaagagc tcgtgagatt ggcatatcag actactaaag tattgagttt gttaggattt
      421 tagcgactag ttagctggga aaggaagata tttgtgacaa ataataaact gtattcgttg
      481 atagaattta gaaataaaat atatgaagaa ttagaacttt ccagaagtga tttagcgatt
      541 ttactatgtg ccatgcttat cgcctctatc ggattaaata tggattcgac tcccgtgatt
      601 attggagcca tgttaatctc tcctttgatg acacctattc tgggagtggg gctctctcta
      661 gctatatttg attttaaatt gttaagaaaa tcttttaaaa tattagctat tcaaattctt
      721 gccagtctaa tagcttcaac actttatttt tatctttctc ccatttcgta tgctagttcg
      781 gagattgttg ctagaacctc tccgactatt tgggatgttc tcattgcttt tgtaggaggg
      841 atagcaggta ttattggtgc taggaaaaaa gagaccaata atattgttcc tggtgttgct
      901 attgcaaccg ccttgatgcc tcctctttgt acagtaggtt atgctattgc ttctgctaat
      961 ctaaaattta tcataggctc ctcttaccta ttcctcatca attgtagctt tattgtcatt
     1021 gcgacttata taggtgttag gttgatgatg gttaagaaac attattttaa agataatgaa
     1081 gaagactcta aaatgcgtag gattttgctt ctagttgctg ttttgctgat gattccgagt
     1141 ttcatctctg caacgacttt agtgagagaa acgttgaaaa aagagtccct taagaaattt
     1201 atatcagagc agtttcaggg gtataatatt ttgaaaaaaa cctattataa aaagactcat
     1261 accctaaagc taaccatttc aggaaattat ttgacagaag aagaactcga tatgatttca
     1321 agtaagagag gtgactatgg tttaagtgat gtttctgttc aagtttcaca attgtctgat
     1381 tcagaacaac ttagtaagga agagctggtg gagtatttct tccagtatat caaggataag
     1441 gaagcaaaag aaaaggagaa agccaataaa tttgatacag agtctgagga gcaataattt
     1501 cttgagaata gctggtttct ctcgtgagtc ttctatgtat atcaaaggaa gactgaggtt
     1561 ttaagtataa aacttttctt ctattatagt agctaagtct tgacagggtt caaaaaatgg
     1621 tttacacttt ataaagttta ttactttgaa aaggtgtgat actgtggcta cggcaacaaa
     1681 aaagaaaaaa tcaacagtta aaaaaaatct agtcatcgtg gagtcgcctg ctaaggccaa
     1741 gacgattgaa aaatatctag gcagaaacta caaggtttta gccagtgtcg ggcatatccg
     1801 tgatttgaag aaatccagta tgtccgtcga tattgaaaat aattatgaac cgcaatatat
     1861 caatatccga ggaaaaggcc ctcttatcaa tgacttgaaa aaagaagcta aaaaagctaa
     1921 taaagttttt ctcgcgagtg acccggaccg tgaaggagaa gcgatttctt ggcatttggc
     1981 ccatattctc aacttggatg aaaatgatgc caaccgtgtg gtcttcaatg aaatcaccaa
     2041 ggatgcagtc aaaaatgctt ttaaagaacc tcgtaagatc gatatggact tggtcgatgc
     2101 ccaacaagct cgtcggatct tggatcgctt ggtagggtat tcgatttcgc ctattttgtg
     2161 gaagaaggtc aagaagggct tgtcagcagg tcgcgttcag tccattgccc ttaaactcat
     2221 cattgaccgt gaaaatgaaa tcaatgcctt ccagccagaa gaatactgga cagttgatgc
     2281 tgtctttaaa aagggaacca aacaatttca tgcttccttc tatggagtag atggtaaaaa
     2341 gatgaaactg accagcaatg acgaagtcaa ggaagtcttg tctcgtctga ctagtaaaga
     2401 cttttcagta gatcaggtgg ataagaaaga gcgcaagcgc aatgctcctt taccctatac
     2461 cacttcatct atgcagatgg atgctgccaa taaaatcaat ttccgtactc gaaaaaccat
     2521 gatggttgcc caacagctct atgaaggaat taatattggt tctggtgttc aaggtttgat
     2581 tacctatatg cgtaccgatt cgactcgtat cagtcctgta gcgcaaaatg aggcggcaag
     2641 cttcattacg gatcgttttg gtagcaagta ttctaagcac ggtagcaagg tcaaaaacgc
     2701 atcaggtgct caggatgccc atgaggctat tcgtccgtca agtgtcttta atacaccaga
     2761 aagcatcgct aagtatctgg acaaggatca gctcaagcta tataccctta tctggaatcg
     2821 ttttgtggct agccagatga cagcggccgt ttttgatacc atggctgtta aattgtctca
     2881 aaatggagtt caatttgctg ccaatggtag tcaggttaag tttgatggtt atcttgccat
     2941 ttataatgat tctgacaaga ataagatgtt accggacatg gttgttggag atgtggtcaa
     3001 acaggtcaat agcaaaccag agcaacattt cacccaaccg cctgcccgtt attctgaagc
     3061 aacactgatt aaaaccttag aggaaaatgg ggttggacgt ccatcaacct acgcgccaac
     3121 cattgaaacc attcagaaac gttattatgt tcgcctggca gccaaacgtt ttgaaccgac
     3181 agagttggga gaaattgtca ataagctcat cgttgaatat ttcccagata tcgtaaacgt
     3241 gaccttcaca gctgaaatgg aaggtaaact ggatgatgtc gaagttggaa aagagcagtg
     3301 gcgacgggtc attgatgcct tttacaaacc attctctaaa gaagttgcca aggctgaaga
     3361 agaaatggaa aaaatccaga ttaaggatga accagctgga tttgactgtg aagtgtgtgg
     3421 cagtccaatg gtcattaaac ttggtcgttt tggtaaattc tacgcttgta gcaatttccc
     3481 agattgccgt catacccaag caatcgtgaa agagattggt gttgagtgtc caagctgtca
     3541 tcagggacaa attattgagc gaaaaaccaa gcgtaatcgc ctattctatg gttgcaatcg
     3601 ctatccagaa tgtgaattta cctcttggga caagcctgtt ggtcgtgact gtccaaaatg
     3661 tggcaacttc ctcatggaga aaaaagtccg tggtggtggc aagcaggttg tttgtagcaa
     3721 aggcgactac gaggaagaaa agattaaata agaggagggg cccgaaagta aatttcaggc
     3781 tccttttggt tagaacttga caaaatacat cattttatgc gaaactagaa gaagattatt
     3841 ttaactagga gaagttatgc gtcttatcta tctgataatt ggttttttat cactggcctt
     3901 ggctattgtt ggggttgttt tacccttgtt gccgacaacg cctttccttt tgttgtctat
     3961 tgcttgtttc tccagaagtt ccaagcgttt cgaagactgg ctttatcata ccaagctcta
     4021 tcaaacatat gtagctgatt ttcgcgagac taagtctatt acgcgtgaac gaaagaaaaa
     4081 aatcatcgtc tctatctacg tcttgatggg aatttctatt tattttgcac ctcttttacc
     4141 agtcaaaatc ggtctgggtg ctttgaccat ctttatcact tattatctct tcaaggtcat
     4201 tccagacaaa gaatagttaa aacagtagtt atttgccttg ataaaattga aagcatattc
     4261 ataacaatat gatataataa atttgaagta atattcaagg agaatcaaat gatttacgaa
     4321 ttttgtgctg aaaatatgac tttacttgaa aaagcgatgc aggctggagc tcgtcggatt
     4381 gaactctgtg ataatctagc agttggtggg acaacaccca gctatggagt gactaaggca
     4441 gcggttgaac tggcagctaa ctacgataca accatcatga ccatgattcg gccacgtggt
     4501 ggtgactttg tctataatga cctagaaatt gctatcatgc tagaagacat tcgtttgact
     4561 gctcaggctg gaagtcaagg ggttgtattt ggagctttaa ctgctgataa aaagttggat
     4621 aagcctaatc tggaaaagtt aattgctgca tcaaaaggaa tggaaattgt ctttcacatg
     4681 gcctttgatg aactaagtga tgaagatcaa ccggaagcta ttgactggct cagtcaagcc
     4741 ggtgtcactc gtatcctaac tcgtgctggt gtgtctggcg actccttaga aaaacgtttt
     4801 gttcactatc acagaatttt ggagtacgct aaaggtaaaa ttgaaattct accaggtggg
     4861 gggattgacc ttgaaaaccg tcaaaccttt atcgaccagg tgggggtaac acaattgcat
     4921 ggtactaagg ttgtttttta aaaaatagaa aggaactgct agcttttggt agcagttttc
     4981 acttatgttt gaaattttta aatcctatca atttaatcaa gaaaaggctc atgattatgg
     5041 ttttatagaa aatagcgaag tctggacata tagttgccag attttgcaag gtgactttgt
     5101 catgactgtg tccatcactg ctgataatgt gaactttcaa gtctttgacc aagagactgg
     5161 tgacctctat cctcacgttc atatggaaag catgagggga agttttgtcg gaaatgtccg
     5221 tgaggcttgt ctggagattc tttaccagat tcggaaggct tgttttgatg tgcaagattt
     5281 tatctgtcat cagactaagc gtatcatgac tcaagttcag gaaaagtatg gaaaccagtt
     5341 ggagtatctg tgggaaaaat cgcctgatac agctgtattg cgccatgaag gcaatcaaaa
     5401 gtggtatgcc gtcttgatga aaatctcttg gaataagctg gaaaagggca gagaaggaca
     5461 agtggaagca gtcaacctca agcatgacca agtagctaat ttgctttcac aaaaggggat
     5521 ttatccagcc ttccatatga gcaagcgcta ctggattagt gtgtcccttg atgatacttt
     5581 atcagatgaa gaagtactgg aattgataga aaaaagttgg aacttaacct ctaaaaaatg
     5641 aaatatttta ataattttca tgaactttca attagctaaa tattctttac tgaagaaatt
     5701 ttcagaaaat atagaattta ccacaccaga ggaatatggt gccatcttca aatacctgat
     5761 tgagaatgtc aagacggatc gtcagatcat ctattcgcct cactgtcatg atgaccttgg
     5821 aatggcagtg gcaaatagcc ttgctgctgt caagaatggt gcaggacgtg ttgaagggac
     5881 tatcaatggt attagggagc gagctggaaa tgctgctttg gaagaaattg cagtggctct
     5941 caatattcgc caagattact accaagtaga aaccagtatt gtcctaaatg agaccatcaa
     6001 tacgtcagaa atggtttctc gcttctctgg tattccagtt cctaaaaaca aagccgtcgt
     6061 tggtggcaat accttctccc acgaatctgg tattcactaa gatggagtcc ttaaaaatcc
     6121 tctcacttat gagatcatca cacctgaatt ggttggtgtt aagattccgc ttggaaaatt
     6181 atctggtcgc catgcttttg ttgagaaact gagagaattg gccctagatt ttacagaaga
     6241 ggatatcaaa tcactctttg ctaagttcaa ggcactggcc gataagaagt aagaaatcac
     6301 agatgcagat attcgagctt tggtagctgg aaccatggtt gaaaatccag aaggcttcca
     6361 ctttgatgat ttacaacttc aaactcatgc agataatgac attgaagcgc tcgttagcct
     6421 agccaatatg gatggtgaga aagtcgaatt taatgcgaca gggcaaggtt ccgttgaagc
     6481 aatctttaac gctatcgata agttctttaa ccaatctgtt cgtttggtgt cctacactat
     6541 cgatgcagta acagatggaa tcgatgccca ggatcgggtt ttggtcactg ttgaaaacag
     6601 agatacagaa accatcttta atgcagcagg gcttgatttt gatgtgttga aggcttctgc
     6661 tattgcctat ataaacgcta atacctttgt tcaaaaagag aatgcaggtg agatgggatg
     6721 cagtgtttct taccatgata tgcctagtgt gtaaaggaga aggctatggc aaagaaaata
     6781 gtagctctag caggagacgg aattggccca gaaatcatgg aggttggttt agaagttctg
     6841 gaggctctag ctgaaaaaac aggttttgac tatgagattg acagacgacc gttcggaggt
     6901 gcagatattg atgcagcatg gcctccctta cctgatgaaa cccttaaggc aagtagggaa
     6961 gcagatgcta tcctactagc agctatcggt agtcctcagt atgatggagc agtggttcgc
     7021 cctgaacaag gcctgatggc tctccgtaag gaactcaatc tttacgctaa tattcgtcct
     7081 gtaaaaatct ttgacagtct caagcatttg tcaccactca aactggaacg aattgctggt
     7141 gtagactttg tcgtggtgcg tgaattgaca ggcgggattt actttggaga tcatattctt
     7201 gaagagcgca atgcgcgtga tatcaacgac tatagctatg aggaagtgga gcggattatt
     7261 cgcaaagcct ttgaaattgc aagaaatcgc agaaaaatcc ttactagtat cgataagcaa
     7321 aatgttctag cgacctcaaa actctggcgg aaagtagctg aggaagtcgc acaggatttc
     7381 ccagatgtaa ccttggaaca ccagctggta gactcagctg ctatgcttat gattaccaat
     7441 cctgctaagt ttgatgttat tgtaacggag aatctttttg gagatatttt atctgatgaa
     7501 tcaagcgtct tatctggtac acttggggtt atgccatcag ccagtcattc cgaaaatgga
     7561 ccaagtctct atgaacctat tcacggttca gcacctgata ttgcaggtca aggaattgcc
     7621 aatcctattt ccatgatttt atcagtttcc atgatgttga gagatagttt cggacgttat
     7681 gaggatgcag agcgtatcaa acgtgctgtt gagacaagtc tggcggcagg aattttaacg
     7741 agagatatag gaggtcaggc ttcaacaaag gaaatgacgg aagctattat tgcaaggtta
     7801 tgaagttaga cgaaaaaatt actctagtcc ttttgatttg gaatgtcatc attttcttga
     7861 tttatggcat tgacaaattc aaggcaagaa gaagagcttg gcgcatccaa gagaaaatct
     7921 tactcatttt agcccttact tgtggtggtt ttggggcctg gttagctgga atcacttttc
     7981 accacaagac tcgaaaatgg tactttaaaa cagtttggtt tcttgggatg gtgaccacac
     8041 tagtagcctt atattttatt tggaggtaat ggatggcagg gtcttcgagg gaacacgctg
     8101 cttgggctct agcggactat ggttttaagg tcgtgattgc aggatctttc ggtgacattc
     8161 attacaataa tgaactcaat aatggcctgt tgccaatcgt tcagcctaga gaggttagag
     8221 agaaactagc ccagctaaaa ccaaccgacc aggtaactgt ggacttggaa caacaaaaaa
     8281 tcatctcacc agttgaagaa ttcaccttcg agatagatag cgagtggaaa cataaactcc
     8341 taaatagttt ggatgatatc ggtattacct tgcagtatga agagttgatt gctgcttatg
     8401 aaaaacaacg accagcctac tggcaggatt agaaaaaata gaaaaggaaa tatagtaaac
     8461 tgaaataaga tgtaaacaaa tgaattagag cttaacatcc atttccagca attttttaga
     8521 aactacagtg gactattctg gattcaacac attataaaat tatgacaaaa cacattcaca
     8581 agaaggctac gacattttaa aaggtgaggg cggatgtatc gtttgcccta ctaaagttgg
     8641 ttacattatc atgaccagca acaaggcagg acttgagcgt aagttcgcag ccaaagaacg
     8701 taagcgtaac aaaccaggtg ttgttctctg cggtagcatg gatgaacttc gcgctttagc
     8761 gcaactcaac ccagaaattg aagcattcta ctaaaaacat tggatgaaga tattcttctt
     8821 ggttgtatcc ttccttggaa accagaagcc tttgaaaaac tcaaagcata cggggatggc
     8881 cgtgaagaac ttatgactga tgtacgtggt actagctgtt ttgttatcaa gtttggaaaa
     8941 gcaggtgaac aattggctgc caagctttgg gaagaaggta aaatggtcta cgcctcatat
     9001 gcttcaatga caaaacgatt gaaactcgct atgagagcaa ggtgtaatgg tgtctatggt
     9061 cgataaggac ggcaaactca tcccagaaca aggaggagca cgttcaactt caccagcacc
     9121 agttgtgatc cgtaaagggc ttgacattga taaaatcatg atgcacctgt cagatacttt
     9181 taactcatgg gactaccgtc aggttgagta ttattaagat aaaaaaagaa gtctagtgtt
     9241 atgagatatt aaagctccta acactggact ttttgtttag aatt
//
LOCUS       DAVMGZ010000080         9271 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000080 DAVMGZ010000000
VERSION     DAVMGZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.081"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..228)
                     /locus_tag="VYQ93_001462"
     CDS             complement(<1..228)
                     /locus_tag="VYQ93_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336131.1"
                     /translation="MNSKIKNILTNFSYVISSNLLTVLTSSLVVLIFPKLMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYLKYGGLEYN"
     gene            complement(256..1590)
                     /locus_tag="VYQ93_001463"
     CDS             complement(256..1590)
                     /locus_tag="VYQ93_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEV3336132.1"
                     /translation="MTYLFLLCLTLFLLTFFYFFAFNQDLIAPPVVMSVMFLISSVFA
                     LINVQNWNIEYSGLAYLLIISGIIVFSMPLLALNSPILNTKIKVTDRLIDIQFWKIAL
                     TIIVDLIILYLYRREIHNLALSHGYTGSNFQWFFRNATSYEGELTVRTSIRVLIRIID
                     VSAYIFGYTFINNFFIYSHKRSKDLLLLVPFLIFISKTLLSGGRLDIIKILIAYVVMA
                     YIQQKRKVGWDKVISHKYMRLGFVGLIAGIPTFYYSLFLSGRSTTRTVFESISTYLGG
                     SIQHFNQYIQNPIGVAEVFGDESFVAIMNILGNLGFVNYNSTVHLEFRQLGITMGNVY
                     TFFRRPWHDFGLVGMYIFSFVVGVFFAIFYLKLRKSRAGFKLDIHTIIYSYFFYWIFL
                     SSIEQYSFTTISLFTLVFIVLVYLMAFFYWNLDFHRGKLVIKLSDTSIKSEN"
     gene            complement(1591..2469)
                     /locus_tag="VYQ93_001464"
     CDS             complement(1591..2469)
                     /locus_tag="VYQ93_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3336133.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNSNTKQIVIIDNFSNNGTG
                     EKLQELYESDSEIDVLINHENAGFARGNNVAYQFAKEKYKPDFMVIMNNDIEIETEEF
                     EKIVTDIYRKEKFHLLGPDIFSTTYQLHQNPKRLTHYTYEEVKALNEKFKKGSQVSLA
                     LKIKCWLKSSKVLRTAIYQNRRKKKSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIKVLHHQNVATNQVYTNLVEKTLFSNKCNF
                     ESTSYFLKLMKENEGV"
     gene            complement(2471..3295)
                     /locus_tag="VYQ93_001465"
     CDS             complement(2471..3295)
                     /locus_tag="VYQ93_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEV3336134.1"
                     /translation="MNQTEIVKLIQKVELDAIKEFQKICKENNIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREGYDKLPGIFKDRIIAGKYQVLAYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSFLRKLYFGKVYWYRFLASLGTTYVGDH
                     VDMHSAKQKLIIGFFKKLGFAKLFPQNYVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDAFFKVPTEYDRYLKRLYGENYLHEEPSDDEKKSHLGGQ"
     gene            complement(3292..4035)
                     /locus_tag="VYQ93_001466"
     CDS             complement(3292..4035)
                     /locus_tag="VYQ93_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV3336135.1"
                     /translation="MNERIQILGITIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCYEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMKHLLELSNEKG
                     YSVYFFGAKEDVLQDMLKIFEERYPALNVVGYRNGYFSPEDEKHIQEDIKEKKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQNAHLEWLFRVAN
                     EPKRLFKRYFVGNVSFIRKVLRAKRGYKL"
     gene            complement(4041..5402)
                     /locus_tag="VYQ93_001467"
     CDS             complement(4041..5402)
                     /locus_tag="VYQ93_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV3336136.1"
                     /translation="MSEKLAKPSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYVLNPFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESDDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRVIDIVGSLVGLILCG
                     LVSIVLVPLIRKDGGSAFFAQTRIGKNGRHFTFYKFRSMCVDAEEKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            complement(5417..6106)
                     /locus_tag="VYQ93_001468"
     CDS             complement(5417..6106)
                     /locus_tag="VYQ93_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEV3336137.1"
                     /translation="MPTLEISQAKLELVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     AGEGKTTTSTNIAWAFARAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIVVDTAPVGVVI
                     DAAIIMQKCDASILVTKAGETKRRDLQKAKEQLEQTGKSCLGVVLNKLDTSVEKYGSY
                     GSYGNYRKQKK"
     gene            complement(6116..6808)
                     /gene="cpsC"
                     /locus_tag="VYQ93_001469"
     CDS             complement(6116..6808)
                     /gene="cpsC"
                     /locus_tag="VYQ93_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV3336138.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKHKLIILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEKVATNLKLD
                     IPAKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIIAVTRVSDVT
                     TLEEARPAMTPSSPNVRRNTLVGFLGGAAVTVITVLLIELLDTRVKRPEDVEDVLQMP
                     LLGVVPDFDKMK"
     gene            complement(6817..7548)
                     /gene="cps4B"
                     /locus_tag="VYQ93_001470"
     CDS             complement(6817..7548)
                     /gene="cps4B"
                     /locus_tag="VYQ93_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEV3336139.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTSDVLDKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLERDLVHIIASDMHNVDSRPPHMAEAYDLVS
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            complement(7550..8995)
                     /locus_tag="VYQ93_001471"
     CDS             complement(7550..8995)
                     /locus_tag="VYQ93_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV3336140.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLMSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEHPDYASKIKKIYTKGFTKKVEAP
                     KTSKDQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALSFVRERYSLADGDRDRGRNQQKVIVAILQKLTSS
                     EALKNYSRIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGMGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDVLEGR"
BASE COUNT         2959 a         1941 c         1471 g         2900 t
ORIGIN      
        1 attatattct aagccaccat atttaagata aatcccatca atccaaccca agtggaaaaa
       61 accgatatag gtcagataaa aaatataaag ttgccagtaa ctgtactctg ttacccccat
      121 taatttgggg aaaattaaga caaccaagga agaggtcaag actgtcaaaa gatttgaaga
      181 aatgacataa gagaaattag ttagtatatt tttaattttg ctattcatca actatcctct
      241 ctatttttat tgttttcagt tttccgattt aatacttgtg tcagagagtt taattactag
      301 ttttcctctg tggaaatcca aattccaata gaagaaagcc attaggtaaa ccaagactat
      361 aaatacaagt gtaaacaaac tgattgttgt aaacgagtat tgctcaatgg acgataaaaa
      421 aatccaataa aagaaataag agtaaataat tgtatgaata tccaatttaa aaccagcacg
      481 gctctttctt aatttcaaat aaaaaatagc aaaaaacaca ccgacaacaa aggaaaagat
      541 atacataccg actaacccaa agtcatgcca gggtcttcta aagaaagtat aaacgttacc
      601 catagtaatc cctaactgcc gaaattctaa gtgaacggta ctattgtaat tgacaaagcc
      661 aagattaccc aaaatattca taatagctac aaatgactca tcgccaaaaa cctcagctac
      721 tccaatagga ttttgaatat actgattaaa atgctgaatc gaacctccta aataggttga
      781 aatactttca aatacagttc tagtcgtaga acgtccagat aagaataaag aatagtaaaa
      841 ggtaggaatc ccagctatca agcctacaaa accaagtctc atatatttat gggagatgac
      901 cttatcccag ccaacttttc gtttttgctg aatataggcc attacaacat acgcaattaa
      961 aatttttata atatccaatc tacccccaga taataaggtt ttagaaataa aaatcaagaa
     1021 tggaactaag agcagtaaat ctttagagcg tttatgacta taaatgaaga aattattaat
     1081 aaaagtatat ccaaaaatat aagcagatac gtcaatgata cgaatgagga cccgaatcga
     1141 agttcgcact gttagctcac cttcataact ggtagcattt ctaaagaacc actgaaaatt
     1201 tgaacccgta taaccatggc taagtgcaag attatgaatc tctctcctat aaagatatag
     1261 aataatgagg tcaactataa tagtaagagc tattttccag aattggatat caatgagccg
     1321 atccgtcact ttaatcttgg tatttagaat aggcgaatta agagccaaga gaggcattga
     1381 aaaaacaata atgccactaa taatcaaaag ataagctaaa ccactatact caatattcca
     1441 gttttgcaca ttaataaggg caaatacaga actaatgaga aacataacag acatgacaac
     1501 tggcggagct atcaaatctt gattgaaagc aaaaaaatag aagaaagtta ataagaataa
     1561 ggtcaggcag agtaaaaata aataagtcat ttacacaccc tcgttttctt tcatcaactt
     1621 caaaaaataa ctagtggatt caaagttgca tttgtttgaa aacaaggttt tttctactaa
     1681 gtttgtatag acttgattgg ttgcaacatt ttgatggtgc aagaccttaa tcttcggtgt
     1741 ataaattctc ttatatcctt ttaactcagc ttcataatct aatatctctg tttcataata
     1801 gaagaaggta ttgggattaa aagcatactc ctctttctca ataaaatctc tagaatatac
     1861 aataaacgaa ccatgaagaa ttgggttttc tacctgtttt ctatagtcta ctgatttctt
     1921 tttacgccta ttttgataga ttgctgtccg aagaacttta ctagacttca accaacattt
     1981 aatttttaat gctagactaa cttggctccc tttcttaaat ttttcattga gagccttaac
     2041 ctcttcataa gtataatgcg tcaaccgttt tgggttttgg tgaagctgat acgtcgtcga
     2101 aaagatatct ggtcctaaca aatggaattt ttccttccga tagatatctg tcacgatttt
     2161 ttcaaactct tctgtttcta tctcaatatc attattcatg ataaccataa aatcaggttt
     2221 gtacttttcc ttagcaaact gatatgctac attattacct cgagcgaaac cagcattttc
     2281 atggttaatc aagacatcaa tctctgaatc tgactcatac agctcttgta gtttttcacc
     2341 cgtaccgtta ttagaaaaat tatcaataat aacgatctgc tttgtattgg aattgccttc
     2401 ttttattgat ttaacacaag aaatagtttc ctctaagact ttgtaatgca aaataatata
     2461 acaaaacaaa ttattgtcct cctaaatgcg atttcttttc atcatcacta ggctcttcgt
     2521 gaagatagtt ttctccgtaa agtcttttca gatagcggtc atactccgtg ggaactttaa
     2581 agaaggcatc ctcaaaaggc ttctctactc cttctcccca aatctctact ggcatgactt
     2641 ctttggcaaa cagagatgca ttaattgtcc cagcatactt ctgctttttc caatcatact
     2701 ttttatagag attatccaag cgtctgtaca cataattttg agggaatagt tttgcaaatc
     2761 ctagcttttt aaagaaacca atgattagtt tttgcttagc ggaatgcata tccacatggt
     2821 ctccaacata ggtagttcct aaacttgcta aaaaacgata ccagtatact ttaccgaagt
     2881 aaagctttct taaaaatgaa tgatttgggg caccatccaa aggaatgata tcaatcaaat
     2941 gcaaacctaa attggtattt cgtggcaatc ccaaacgttt tctttcatct tctaaaagga
     3001 ataagcgagg aaagtagcaa tgtaaagtat cgcaatattg ataagcaaga acctgatatt
     3061 tgcctgcgat aattctatcc ttaaagatac ctggaagttt atcgtagcct tcacgaggca
     3121 cagcaatatc catgtcatca tcccatggaa taaagccatc atatttgact gccccaagta
     3181 cactcccccc acgaaggaag aaatctatat tgttctcttt acaaattttt tgaaactctt
     3241 ttatagcatc caattcaact ttctgaatca atttaactat ttctgtttgg ttcaaagttt
     3301 atatcccctt ttcgctctta aaacttttct tataaatgaa acattcccta caaaataacg
     3361 cttaaagaga cgtttaggct catttgccac acgaaacaac cactctaggt gagcattttg
     3421 catccataat ggagctcgtt tgatatgccc tgatagtaca tcaaaacttc caccaacccc
     3481 cataaacaca gcattaattc cattatccat gaatgattgg ataatatatt ctttcttagg
     3541 agaagtaatt cctacaaaca caaaatctgg ttttttctct ttaatatctt cctgaatatg
     3601 tttttcatct tcaggcgaaa aatatccatt tctatagcca acaacattta gagctggata
     3661 tctttcttca aaaattttta acatatcttg taaaacgtct tcttttgctc caaaaaagta
     3721 gaccgagtat cctttttcat tagacaactc tagtaaatgt ttcatcaagt ctattccagc
     3781 aactcgctca ggaactggtg ttcctaaaaa cttactcgct aaaactactg aagcaccgtc
     3841 ggcattaatt attcctgatt cattaacgat ttttttgatt ttctcatctt catagcactg
     3901 attaatttta tccgcgttaa ctcccatcaa gtgcaaaggg tgcttcttta aaacatactg
     3961 ttcaacagca tccactgttt ctttcatcgt taatggatct atagtaattc ctaatatttg
     4021 aattctctca ttcatatgaa ctatttcgct ccatccttca tcaatacaac cttaactgtc
     4081 ttcaataaaa tttcaatatc tttccagatt gtccaaccat caatataggc cacatctaat
     4141 ttgacaactt catcgaaatt tttgatttca cttcgtccgc tgacctgcca taaacctgtt
     4201 atgccaggtt taaaacttag gcgacgtttt tgttctgggg tatagtgctc atactcgtcc
     4261 actgttggtg gtcgtgtacc taccaaactc atatctccct ttagaacatt ataaaactgt
     4321 ggtagctcgt ccaagctcgt cttccgtata aaatgaccaa ttttcgtgat acgaggatca
     4381 tcgtccacct taaacattcc accctgcatg gtattttgtt ccatgagttc tctttttttc
     4441 tcctcagcat ccacacacat agagcgaaac ttataaaaag taaaatggcg gccatttttt
     4501 cctatacgcg tctgagcaaa aaaagcagaa cccccatcct ttcgaatcaa aggaaccagc
     4561 acaatactga ctagaccaca taatatcaaa cctaccaggg aaccgacaat atcaataacc
     4621 cgcttagcaa tcacatggct agtcttataa aatgttgtag aaaaagtcac aacatttagt
     4681 cctgccatct cacaaatttg cttgttacgt gcccaatcga aagcatttag attgactgtt
     4741 acatcaattc ccatcgtttc aaactgagag acaagctctc caatattgta tttttcactt
     4801 ggaagattga taaagacttc atcgaccacc tcatgagtcg caaaatttac tatctccccc
     4861 tctgctacaa cctttaaatc atcatgctga aaatctggtt tatctaaaac actgacggct
     4921 accaactccc caacaacatc atctgattct attagtctat ccagtacctt ttcaacacga
     4981 gaagttgctg taagtaggag aatcttctta cttcctttaa agttgggata agcccgcttc
     5041 caataccact tgataaatgg gtttagcaca tagactaaga aaacatgtaa taagaggaag
     5101 taaatcatgc ctcttctgga aatactaaat cgatcttcta agaaaaaatt agaaatacta
     5161 atcgctagcg caaagaatag gatatatttc aatgtctgga caagttcaat caaatatccc
     5221 cttttaaaga aattccgtcc ataatcactg atataaaaga caaaatagtg gaggatataa
     5281 agtacaatag ctgttgttga aacaatctct gtttctctca cagcgctaag tagataagtc
     5341 aataaaataa caagaaaact ctggactatg gctactgaag gctttgctag tttttcactc
     5401 atttctatcc ctcaacctat tttttctgtt tcctgtaatt cccataactc ccataagaac
     5461 cgtatttctc tactgaagta tcgagtttat tcaacacaac tcctaaacac gacttcccag
     5521 tttgttccaa ctgttctttc gctttttgaa gatcccgtcg ttttgtttca cctgccttcg
     5581 tcactaaaat agaagcatca catttctgca taatgatagc cgcatcaatc acgacaccga
     5641 caggggcagt gtctacgacg atatagtcaa aatatttacg caaggtttca agcattgtac
     5701 tgaaattctt actttgaaga agggctgtcg gatttggtga cacagagcca gcctgaatca
     5761 caaagagatt ctcaatattg gtatcgcaaa gcccctgtga caggtctgtt gtccctgata
     5821 gaaattctgt tagtccagta atcctttccc ttgatttaaa gacacctgac ataacggagt
     5881 tacgcatgtc tgcatcaatc agaagcgttt tgtaacctgc acgcgcaaaa gcccaagcga
     5941 tattggtgga agtcgttgtt tttccttctc ctgctttcac agaagtgata gaaaatactt
     6001 tcaaatcatc tccacttaac tgtaggttcg tgcacaaagc attataatat tcctctgcct
     6061 ttttcacaag ttctaatttc gcctgtgaga tttctaacgt tggcataact tcctcctatt
     6121 tcattttatc aaaatctgga acgactccta gaagtggcat ttgcagtaca tcttcaacat
     6181 cttcaggacg ttttacacgg gtatcgagca actcaatcaa aagaacagta attactgtta
     6241 cggcggctcc tccaagaaaa ccaactaagg tgttgcgtcg aacatttgga gaagagggag
     6301 tcatagctgg tcgcgcttct tcaagcgtcg ttacatcaga tactcgcgtt acagcgatga
     6361 tcttttctgc agcaacttct cgtagagaat tagcaatgcg actggcttcc tctggttgtt
     6421 tatccttgac agagattgag acgatacgag tgtcggctgg tacagtcact tgcactttgc
     6481 tagttaacgt ttttgctgga atatccaatt tcaaatttgt cgctaccttt tccaatacat
     6541 cttgcgaaag gataatttcg cggtagtctt ttaccagata agttcctgcc tgcaagtcct
     6601 gattcgtcag tcccggctta tctccttgat tacggttgac tacgtaaata cgcgtggtgc
     6661 ttgtatattc tggcttaaca ataaaagtgc tatatgcaaa agctcccgcc cctgtcacaa
     6721 gtgccactaa taaaattatt agcttgtgtt tccacaaggt tttaagtaat tgaaatacat
     6781 cgatttctat cgtgttttgt tctttcatca tttctcctaa attagttgat ccattacaat
     6841 ttttcgagga ttatctataa aaagttcctg agccttcgct tctccgtatt tttgggaaac
     6901 aaggtcatat gcttctgcca tatggggagg tctgctgtcc acattatgca tatcacttgc
     6961 aatgatatga accaagtcac gctccaaaaa atactgagct ctttttttca tgaatttata
     7021 acgttctcca aaaagtttgg atttgaggac atgtgaacta tttacttgcg tgtaacagcc
     7081 catattgatc agctctcgaa cgcgtttttc attattttca agtgcatcat agcgctcaat
     7141 gtgggcaatg actggagtaa ttcccaacat caatatttta ctcaaggcac tatgaatatc
     7201 gcgataaggg gtgttcatac taaactctat caaggcataa cgactattat tgagggtcgg
     7261 aatccgattt ttttccagct tatccaaaac atctgatgtg tagtaaattt cagccccgta
     7321 agcaatgacc aagtcactcg ccacttcctt agctatttcc cgaacctgaa gaaagttttc
     7381 tgctatcttc tcttccggag tttcaaacat gcccttgcga cggtgagagg tagagacaat
     7441 ggttcgcacc ccctgcctgt aggattctgc caagagagcc ttgctttcct ctcttgactt
     7501 gggaccatca tctacatcaa agacgatgtg cgaatgaata tcaatcattt catctgccct
     7561 ccaacacatc ctgaatagca gttttgacag atgcaaggct actgtcgtta atttccatca
     7621 tatagaggtt actatctggc atcgcatagg aaggaagatc cgtccgtccc atacccttca
     7681 agtcttgcga attcactttg tacgttccac cactttctaa ctgagcattg accaagttta
     7741 tcatggtctc aagtggcatg tttgtttgga tagaatcttg caagctatca atgatcctac
     7801 tataattttt cagcgcttcg ctcgaagtta atttttgaag gatagccaca atcacctttt
     7861 gttggttgcg accacggtca cggtctccat cggctagtga gtagcgttca cgtacaaaac
     7921 ttagagcctg ctcagagtct aggtggacat tccctactgg gaaatggaac ttcccatgta
     7981 gagctgaaaa ctcttgatca ttatgaacat ctacccctcc caataagtca atcattttca
     8041 agaaagaagt gaagttcaat cgcacatagt aattgatatc cactccatag agattttcta
     8101 aggtgtgaat ggacgaatca actccataaa tgcccgcatg ggtcaattta tctttttgat
     8161 tattcccacc atctgcgatt ggtacataag catcacgtgg cgttgtggtc aagagaattt
     8221 tcttggtatc tcgattgaca gtcatcagga tattgacatc tgaacgcgac accgaactaa
     8281 tcggaccata agtatcaatt ccactaacat agatattgaa agactgatcc ttagacgtct
     8341 taggagcttc tactttttta gtgaatccct tggtataaat cttttttatc ttcgatgcgt
     8401 aatctggatg ctccgattcg atgatatttt caaagacact atttaagaca atggccttag
     8461 tctctcctgc aatcaaactc ttataagctg ccaagtaaga cgaactctga ttgaccgtca
     8521 aatcggtatt ctgacttgat ttgatatcag ctagtagttt ttgaatgttt tcattatcag
     8581 tcccagtcgg tgctgtcaca ctcatcagtt gcgtaacatt ctcaatctca ctatctgcta
     8641 aaacagcaac actgatcgaa tattctgagt aattagaagt cgcatttaaa cgattggtca
     8701 gtcccacaaa ctgctgtact gcaaagagcg acacagagct gacaaggata gagaacacca
     8761 acagaaaaat agtaaacttt tcagcttttt tatagataat caagagtagc cctaccaagg
     8821 caactagtag gactaacgca gttaccacta ggttaagata tctaaaagca aggatattgt
     8881 acttaaagat taagaacaat aaaaaacaaa ctaacaataa ataaatagtc agcaaaacga
     8941 tattaacact tcgcttcact ttctgtgaac ctgatttttt aaaacgtcta ctcatgatta
     9001 acacctatac attgaacatc ttatgattat atcacttttt tacggtaatg tctacatttt
     9061 tttactatct gcatcttttt ttactatctg catctttaag tatcttagta gacttcccgc
     9121 gaaacaaaaa tatagtaaaa tgaaataaga acatgacaaa tcgatcagga cagtcaaatc
     9181 gatttctaac aatgttttag aagcagaggt ctactactct agtttcaatc taattatgaa
     9241 caagacccag aaaagtagcc ttatttctta a
//
LOCUS       DAVMGZ010000081         9158 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000081 DAVMGZ010000000
VERSION     DAVMGZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.082"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            150..470
                     /locus_tag="VYQ93_001472"
     CDS             150..470
                     /locus_tag="VYQ93_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV3336141.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            474..1289
                     /locus_tag="VYQ93_001473"
     CDS             474..1289
                     /locus_tag="VYQ93_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336142.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSIFTISLVIGIWNWKEAVFRPFIS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            1364..2290
                     /locus_tag="VYQ93_001474"
     CDS             1364..2290
                     /locus_tag="VYQ93_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV3336143.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDVLMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            2356..2622
                     /locus_tag="VYQ93_001475"
     CDS             2356..2622
                     /locus_tag="VYQ93_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV3336144.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            2615..3313
                     /locus_tag="VYQ93_001476"
     CDS             2615..3313
                     /locus_tag="VYQ93_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV3336145.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKSLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            3324..3803
                     /locus_tag="VYQ93_001477"
     CDS             3324..3803
                     /locus_tag="VYQ93_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3336146.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQYRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            3811..4539
                     /locus_tag="VYQ93_001478"
     CDS             3811..4539
                     /locus_tag="VYQ93_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3336147.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            4581..5450
                     /locus_tag="VYQ93_001479"
     CDS             4581..5450
                     /locus_tag="VYQ93_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV3336148.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            5425..5865
                     /locus_tag="VYQ93_001480"
     CDS             5425..5865
                     /locus_tag="VYQ93_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV3336149.1"
                     /translation="MIPQEMWNKYKQINPSIGDEIDAWAFGVEPDLLGDLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            5988..8534
                     /locus_tag="VYQ93_001481"
     CDS             5988..8534
                     /locus_tag="VYQ93_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV3336150.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(8592..>9158)
                     /locus_tag="VYQ93_001482"
     CDS             complement(8592..>9158)
                     /locus_tag="VYQ93_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEV3336151.1"
                     /translation="LDRFHIIQHLSRAMSRFRVQIMNQFERKSHEYKAIKRYWKLIQQ
                     DSRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHFQNKDPEKFF
                     GLIEDNLKQVHPIFQTVFKTFLKNKEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGF
                     RNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         2752 a         1544 c         2079 g         2783 t
ORIGIN      
        1 aaccatacat acggtagggc gacgctgacg tggtttgaag agattttcga agagtattaa
       61 ctattatgct ttacaaacta gtgtagaagt aatatactat aggtgaagtt actagtaggt
      121 attacaaaat agattagaag ggagaagtga tgaaagaaac tcagctatta aaaggtgttc
      181 ttgaaggttg tgtcttggat atgattggtc aaaaagagcg gtatggttat gagttggttc
      241 agactttgcg agaggctgga tttgatacta tcgttccagg aactatttat cctttgttgc
      301 aaaagttaga aaaaaatcaa tggataagag gcgacatgcg cccgtcgcca gatggtccag
      361 atcggaagta tttttcatta atgaaagaag gagaagagcg tgtctcagtc ttttggcaac
      421 aatgggacga tttgagtcaa aaagtagaag ggattaagaa tgggggttaa accatgaaga
      481 aaatgaagta ttacgaagaa acaagcgctt tgctacatga gttttctgag gagaatcaaa
      541 agtattttga ggagttgtgg gaaagtttta atcttgctgg atttctctat gatgaagact
      601 atctcagaga gcagatctat ttgatgatgc tagatttctc agaagcagaa cgagatggca
      661 tgagtgcaga ggattatcta ggtaagaatc ctaaaaaaat aatgaaagag attctcaagg
      721 gagcacctcg cagttctatc aaagagtccc ttttgacgcc aattcttgtc ctggcggtat
      781 tacgttatta tcaactacta agtgattttt ctaaaggtcc tctcttaaca gtcaatttgc
      841 tcacattttt agggcaactt cttatttttc tgattggatt tggacttgtg gccacaattt
      901 tacgaagaag tttagtccaa gattctccta aaatgaaaat tggcacttac attgttgttg
      961 ggactatagt tcttctagtt gttttaggat atgtaggaat ggcaagcttc atacaagaag
     1021 gagcctttta tattccggct ccctgggata gtttgtctat ctttacgatt tcgctagtta
     1081 tcggtatttg gaattggaaa gaagcggtct ttcgtccatt tatcagtatg attattgccc
     1141 atcttgtggt gggttctctg ctccgttatt atgagtggat gggaatttca aatgttttcc
     1201 ttacaaaagt tattccttta gctgtcctct ttattggaat ctttgtcttg ttccgtgggt
     1261 ttaagaagat aaaatggagt gaagtatagt caaaaagtcg ttgcaaagcg gttttttgtt
     1321 ataatgaagg gaagaatgtt agaaattaaa ggagaaaaat ttgatgagat acataactct
     1381 tggtcaagat gacaaagaat tatcagaaat tgttctcgga atgatgagaa taaaagataa
     1441 atctgtaaaa gaagttgaag agcttgtaga aacagcactt tctgttggaa tcaatgcctt
     1501 cgacttggct gatatttatg gtcgtggtcg ttgtgaagaa ctgttaggtc ttgtcctaaa
     1561 aaatcgtcca gatttaagag aaaagatgtg gattcagtcc aaatgtggca ttcgcattga
     1621 agaatttacc tattttgatt tttctaagga ctatattata aaatcagtag acggtatttt
     1681 gcaaagattg aagattgatc atctagatag cttgctcctt catcgaccag atgttttgat
     1741 ggaatctgac caagtagcag aagcctttaa tctcctttat aaacaaggta aagttcgaga
     1801 ttttggagtt tctaatcaaa atcctatgat gatggagttg cttaaaaaag atgtcaagca
     1861 gccgttagct gttaatcagc tacaattgag tgcggctttt actccaggat ttgaatcagc
     1921 ttttcatgtt aatatggaag atagtcaagc agctatgcga gatggcagca tttttgaata
     1981 ttgccaatta cacgatgtgg tcattcaagc atggtctgtc ttacaattcg ggtattttaa
     2041 agggaatttt gttggaaatg agaaatttca agctttaaat caagtacttg atcgtttagc
     2101 tattaaatat ggagtaacct cttcaactat tgccatttct tggatattgc gttatccagc
     2161 aaaaatgcag gcagtcgtag gtacaacaaa tcctaagcac ttgagagaag ttagccgagc
     2221 ggcaaacttt agcttaacaa gaaaagaatg gtatgaaatt tatcttgctg cagggaataa
     2281 tttgccgtaa gtagtagcag gtttgaataa ctcacattca aaaagccgta taaagcggtt
     2341 ttttgctata attaaatgaa gaatgtagaa ttaaaggaga aaaacatgac atttgaagag
     2401 atcctgcctg ggttaaaggc taagagaaaa tatgtacgaa ctggttgggg aggtgcggaa
     2461 aactatgtcc aactctttga taccatcgag caaaatgggc ttgcacttga aatgacacct
     2521 tatttcctaa tcaacgtttc tggcgaagga gaaggttttt ccatgtggag cccgacagtt
     2581 tgtgatgttt tggcaacgga ttgggtagaa gtgcatgact aaacgtgtac tcattacagg
     2641 agtgagttca gggatcggat tggctcaagc tcgcctcttt ttagagaagg gctatcaagt
     2701 ttatggagtt gaccaaggtg aaaagtcact cttagagggt gattttcact ttttacagag
     2761 agatttgacc ttggacttgg aacctatttt tgactggtgc cctcaggtgg atgttttgtg
     2821 taatactgct ggagttttgg atgattacaa accactgttg gaacaaacgg cgcaggacat
     2881 tcaagagatt tttgaaatca actacattat tccagtagag ttgactcggt attatttgac
     2941 acaaatgctg gaaaataaaa agggaatcat catcaatatg tgttccattg cttctagcct
     3001 agcaggtgga ggtggtcacg cctatacttc atccaagcat gccttggctg gcttcaccaa
     3061 gcagttggct ctagactatg ctgaagctgg gattcaggtc tttggtatcg ctccaggagc
     3121 agtcaagaca gctatgaccg ctgcggattt tgaaccaggt ggtttggctg actgggtggc
     3181 tagtgaaacc ccaatcaagc gctggattga gccagaggaa atagcagagc ttagtctttt
     3241 tttagcaagt ggaaaagcga gcgccatgca aggacaaatc ttgacaatag atggtggctg
     3301 gtctttgaag taggaggagt tggatggaaa tcaaaaaaca ctttggagtc tacgctgttt
     3361 gcttagaaaa tgggaagtta ctctgcattg aaaaaacgag aggcccttat caatatcggt
     3421 atgatctacc tggaggcagt cagcaacttg gtgaaggact gacggaaacg ctgactagag
     3481 aagttatgga agagacggga tttactgtta gaagctactc taatcctcga atctacgatg
     3541 ttttcgttag ggaagagtta aaaaatttta tggttcacca tgtcatggcc ttgtatgatg
     3601 ttgaaatgaa tgagagtgca cctcaagtta cgacttcgga agctgtgtct gatggtgcga
     3661 atgattcact tggatatatt tggatggata ttcaagagat tacagaagaa aatgcatcgc
     3721 cattagtctt gaaggttaag tctgaattat taggatttcc agaactggac aagacttctt
     3781 acatgaattg gaaggtgaag tagagggata atggaactat ttaaaacatg gaagaaaaat
     3841 atggttctct atggtcttaa atctcaaatt ggaactgtct accgaaacaa tgataggaca
     3901 acaagctttt atgatgttgg gaattttcta tacctagcag gggagttgga ttccagattt
     3961 tgggaagatt ttgttagaaa atatggttta gattataaga ttattatttc agaaaatact
     4021 aattggcaag attttctgca tcgaaaagtg gggctaaatt cttttactcg ctattctttt
     4081 aaagataagg caaattttca agttgaattt ttaaataatc tagttactca tttagaggaa
     4141 ggttacaata ttgtgcctat tgataatcat atttataact gcttttctac ggaagaatgg
     4201 tcacaggatt tacaggggga ttttgagtcc tatcaggatt ttgttttaaa aggtggattt
     4261 ggctttgtga ttcttaaaaa taatgaactg attgctggga tttcctcagg gttagtttac
     4321 cgtaaagcag ttgaagtgga agttgcaact agaccaaacg aacaaggaaa tggatttgct
     4381 aaaaagcttg gtgctgcaat gattctagag agtttaaata gagatatgtt tccactttgg
     4441 gatgctcata atgaggcttc caaaaaagta gcagaatttt taggatatga gttatctgaa
     4501 ccttatgaag cttttgaact agaggaaatt ttgatataat tatatatgat attgtatctt
     4561 ttaagacgag gtgaatggct atgagaaatc aacttgccct tagtggcgaa aaaattcttg
     4621 aaaaaattta tcctcaacta tttcaccacg tcggcatgat tcgtggggaa tacttattga
     4681 gagagcttaa tcaaaacatc atattagcaa gttgtcagca atttgtaaaa gattatcttg
     4741 atactatttg ctccttttac ttagataagg aagtttggta tcgtttttct gaattaacga
     4801 atacagaagc caattgttta gcggggacta gagagttttt tgacgaaggg catcccttgt
     4861 ttggctatag aggaaccagg cgtttactgg cgtgtttgga tgaatttcag gctgaagcac
     4921 atgtcgttac agaagtttat caaaccaatc ccaatctgtc tcttattttt ccttttgtca
     4981 atgatgccga ccaattaaaa caagctatta cagtattgcg tcagtatggc tttactggga
     5041 aagtcggaac gatgattgaa ttaccgtcag cgtattttga cttatctagt atactggaaa
     5101 cgggcatttc aaagattgta gttggaatga atgatttgac ttcctttgtt tttgcgacta
     5161 tgagaaacag tcaatggcat gatatggaaa gtccaataat gttagatatg ctaagagata
     5221 tgcaggataa agcaagaaag aacaagatta attttgctgt agcaggctat ctgaatactt
     5281 ctttcataca aaaaatgaat cagttgggta tcaagtgcat tatccactac agttctattc
     5341 cagagatttt tgatttagag attgaccatc cagatcatct taaacacata aaagaagaaa
     5401 gtaaaaaatt acaaaggagt acccatgata ccgcaagaaa tgtggaataa atacaagcaa
     5461 atcaatcctt ctatcggaga tgaaatcgat gcttgggctt ttggagttga accagacctt
     5521 ttaggggatt tggtgtttaa aggagaaaag acagcaacag catcagccta tgacctctat
     5581 gtactagaag acgaaccact tccccaagta gggaccttcg atgtcatttt agatagtcaa
     5641 aatcaggctg tctgcattgt cgaaattaca aaggtttctg ttgaactctt caatcaagtt
     5701 tctgcgcaac atgcctttaa ggaaggtgag ggagacaaat cacttgccta ttggcgccag
     5761 gttcatgagg actttttcac agactgtttg ggtgaagtag ggctgacttt tacacctgaa
     5821 agcaaggttg ttttagaaga atttcgcaag gtctacccac tgtagactat tagaaggaag
     5881 aaagttttgg aaatcgctgt ccaatccttt tttctcaagc aaaatatgat ataataagtt
     5941 tgtttgaaga agagcagcag ctcttaaact tagaatagga gaaaactatg caagcagttg
     6001 aacattttat taagcaattt gttcctgaac attatgattt atttttagat ttgagtcgtg
     6061 agaccaagac tttttctggg aaagtgacca tcactggtca agcacagagt gaccgcatct
     6121 ccctccacca aaaagacttg gaaatcacct ctgtagaagt tgcaggtcaa gctcgtccat
     6181 ttacagttga ccatgacaat gaagcccttc atatcgaatt ggctgaggct ggtcaagttg
     6241 aattggttct tgccttttct ggtaaaatta cagacaacat gacagggatt tacccttctt
     6301 attatacagt tgatggagtc aagaaggagg tcttgtctac tcagttcgag agccattttg
     6361 cgcgcgaagc tttcccatgt gtggatgagc ctgaagccaa agcaactttt gacctctctc
     6421 ttcgctttga ccaagcagaa ggtgaattgg ccttgtcaaa catgccagaa atcgatgttg
     6481 aaaaccgtaa ggaaacaggt atctggaagt ttgagacaac acctcgcatg tcttcttact
     6541 tgttggcctt tgttgctggt gatttgcaag gggtgaccgc taaaactaaa aatggtaccc
     6601 tggtaggtgt ctactcaacc aaagcacatc cactttcaaa tcttgatttc tcactggata
     6661 tcgctgttcg ctctatcgag ttttacgaag attactatgg agttaagtac ccaattcctc
     6721 aatctctcca catcgccctt cctgacttct cagctggtgc tatggaaaac tggggtcttg
     6781 tgacctaccg tgaagtttac ttggttgtcg atgagaactc tacatttgct agccgtcaac
     6841 aagttgccct tgttgtggcc catgaattgg ctcaccaatg gtttgggaac ctcgtgacta
     6901 tgaaatggtg ggatgacctt tggctcaatg aaagtttcgc taatatgatg gaatacgtct
     6961 gtgtggatac catcgaacca agctggaata tctttgaaga tttccaaaca ggtggagtac
     7021 ctcttgctct tgaacgtgac gctactgatg gcgttcagtc tgtccacgtc gaagttaaac
     7081 atccagatga aatcaataca ctctttgacg gcgctatcgt ctatgccaaa ggaagccgtc
     7141 tcatgcacat gcttcgccgt tggctaggag atgctgattt tgctaaaggt ttgcatgcct
     7201 actttgaaaa acaccaatac agcaacacca ttggtagtga cctttgggat gcccttggtc
     7261 aagcgtcagg acgtgatgtc gcagccttca tggattcttg gttggaacag cctggttacc
     7321 cagttcttac tgtcaaagtt gaaaatgatg tcttgaagat ttcacaaaaa caattcttca
     7381 tcggtgagaa cgaagacaag aaccgtctct gggtggtgcc cctcaatagc aactggaaag
     7441 gcttgcctga tacactcgaa actgaaagta tcgaaatccc tggctacgca gctcttcttg
     7501 ctgaaaatga aggagctctt cgtctcaaca ctgaaaatac tgcccactat attaccgact
     7561 atcaaggaga cttgttagaa gctgttcttg ctgagctaga gacacttgat aacacaagca
     7621 aactgcaaat tgttcaagaa cgtcgtttgt tggctgaggc agggcacatt tcttatgcag
     7681 acttgcttcc agttcttgat aaacttgcta aggaagagtc ttatctggtg gtttcagctg
     7741 tttctcaagt gatttcagcc cttgagcgct ttattgatga aggaacggat gctgaaacag
     7801 cctttaaagg actggttgct aaattggctc gtcataacta tgaccgtctt ggttttgaag
     7861 ctaaagacgg agaatcagat gaggatgaat tggttcgtca gctggctgtt tcgatgatga
     7921 ttcgctcaaa tgatgcagaa gctagtcaag tcgctagcca aatcttcgca acacacaagg
     7981 agaatcttgc aggactccca gcagctattc gctcacaagt tctgattaat gagatgaaac
     8041 atcatgagac taaagacttg ttagcacttt atcttgatac ttatactcac gcaacagatg
     8101 ctgtctttaa acgccagttg gcagcagctc ttgcatacag tacagatgcg gataatatcc
     8161 aaaacctgat tacttcttgg aaggacaaat ttgtggtcaa accacaggac ttgtctgctt
     8221 ggtattacca attcctagct catcaagcaa ctcagaaaac agcttggtct tgggcgcgtg
     8281 aaaactgggc ttggattaag gcagcccttg gaggagatat gagctttgat agctttgtta
     8341 tccttcctgc tcatgtattt aagactcagc aacgcttggc ggaatacaag gagttctttg
     8401 agccgcagct ttctgacctt gctcttagcc gtaacatcgg tatgggaatc aaggaaattg
     8461 cagcacgtgt tgacttgatt agccgtgaaa aagctgcagt cgaagcagtt gttcttcaat
     8521 acggaaatgc ataaataagc ctaaaataaa aagaaaaggc tctttgtcaa ctgtagtggg
     8581 ttgaaaaaaa gctaagctcg agaaaggaca aattttgtcc tttctttttt gatattcaga
     8641 gcgataaaaa tccgtttttt gaagttttca aagtttcgaa aaccaaaggc attgcgtttg
     8701 ataagtttga tgagattatt ggtcgcttcc aatttggcgt ttgaataggg tagttgaagg
     8761 gcgttgacga ttttctcttt gttctttaga aaggttttaa agacagtctg aaaaatagga
     8821 tgaacctgct tcagattgtc ctcaatgagt ccgaaaaatt tctcagggtc tttgttctga
     8881 aagtgaaaaa gtaagagttg atagatctga tagtggtgtt tcaagtcttc tgaatagctt
     8941 aaaatcttgt caagaatttc tttatttgtt aagtgcatgc gaaaagtagg gcgataaaaa
     9001 cgtttatcgc tgagtttacg actatcctgt tggatgagtt tccagtaacg cttgatagcc
     9061 ttgtattcat gagattttcg ttcaaactga ttcataattt gaacacgaaa acgactcatg
     9121 gcacggctga gatgttggat aatatggaaa cgatctag
//
LOCUS       DAVMGZ010000082         9056 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000082 DAVMGZ010000000
VERSION     DAVMGZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.083"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..264
                     /locus_tag="VYQ93_001483"
     CDS             <1..264
                     /locus_tag="VYQ93_001483"
                     /inference="COORDINATES: protein motif:HMM:NF019216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336152.1"
                     /translation="EDKFGKLNKVSFIDPNGSWARQQKVTIDNINRLEKMIEDAVKFD
                     AEDEVAKLYPETNSRVLKLKKAIFKAYLDQTGDFRSSIFENKK"
     gene            308..5521
                     /locus_tag="VYQ93_001484"
     CDS             308..5521
                     /locus_tag="VYQ93_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV3336153.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNMDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            5793..6644
                     /gene="ylqF"
                     /locus_tag="VYQ93_001485"
     CDS             5793..6644
                     /gene="ylqF"
                     /locus_tag="VYQ93_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV3336154.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            6631..7410
                     /locus_tag="VYQ93_001486"
     CDS             6631..7410
                     /locus_tag="VYQ93_001486"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV3336155.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            7426..8976
                     /locus_tag="VYQ93_001487"
     CDS             7426..8976
                     /locus_tag="VYQ93_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV3336156.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNILITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3030 a         1540 c         1879 g         2607 t
ORIGIN      
        1 gaagataaat ttggaaagtt aaataaagtt agttttatcg atccaaacgg ttcttgggct
       61 cgacaacaga aagtgactat tgataatatt aatagacttg aaaaaatgat tgaagatgca
      121 gtaaaatttg atgctgagga tgaagttgcc aaattatatc ctgagacaaa tagtagagtt
      181 cttaaactga aaaaagcaat ctttaaagct tatcttgacc aaaccggtga ttttagaagt
      241 tcaatttttg agaataaaaa atagtgttta ctattaggaa ataaaaagtg aaggtggagg
      301 attagaattg tcattattaa aaaaagataa attttccatt cggaaaataa agggtattgt
      361 tggttcggta ttcttgggga gtcttttatt tgcaccgtca gttgttggtg catcgactta
      421 tcattactta gattatagta gtttgacaca aactgaacgt gatcaactca aacaaggtag
      481 acctgatgaa tccaaagagt catatgcttt ggattatgaa aaagacgcac taccaaacac
      541 aggaagttct caatctatta tgactgcatt gggtttgtta gctatcggga gtcttattgt
      601 gattatcaca aaagataata gaaataaaaa aatagctaca tttttgattg taggggcgac
      661 aggtttagtt acactatcaa ctgcttcagc acttaatttg aatgctaaca tccatgagtc
      721 tggacgtgat ggtgtgttgc aaatttctgg ttacagatat gttggttact tggagcttga
      781 tgataaaact gtttcgtcgg tttctccagc atcaactgtt tcgccagttg agcaacccaa
      841 agttgtaaca gagaaaggcg aaccggaagt tcatgagaag cctgactata cgcaaccaat
      901 aggtgcaaac cttgttgagc ctgaagttca tgagaaacta gcatacactg aaccagtagg
      961 gacaacagga gtagatgaaa atggaaactt gattgaacca cctgttaacg acatcccaga
     1021 atatactgag ccagtaggta caacaggagt ggatgaaaat ggaaacttga ttgagccacc
     1081 tgttagcgac attccagagt atactgaacc aatatctaca gtttcagaag ttgcatcaga
     1141 aagagaagag ttgccttctt tacataccga tattcggaca gagactattc ctaaaaccac
     1201 tatagaagaa tctgatccta gcaaatttat aggcgatgat tctgttagac aagttggtga
     1261 ggatggcgaa cgtcaaattg ttactagcta tgaagagtta catggcaaaa aaatcagtga
     1321 cccagttgaa acagtaacta ttttgaaaga aatgaagcct aaaattcttg taaaaggtac
     1381 caaagaaaaa cctaaagaaa aaacggctcc tgttttgacc ttagatagaa caaatacaaa
     1441 tgtattaaac cgatccgcaa ctctttctta tcatttagtt aatacagatg gagtcacaat
     1501 caataaaatt actgcgacaa ttaaagatgg aaatgaaatt gtcaaaacag tagatttgac
     1561 ttcagagcaa ttagataagc aggtagaaga tttaaaattc tacaaggact ataagataga
     1621 gaccactatg acctatgacc gtggcaaggg agaagaaact gcaacattag aagagaaacc
     1681 acttcgcttg gatttgaaaa aagttgaaat taaaaatatc gcatctacta atttagtgaa
     1741 ggtaaatgac gatggtactg aaacacctag tgattttatg actgaaaaac cttcagatga
     1801 agatgtgaag aaaatgtact taaaaattac tagtcgtgat aataaagtaa cacgtttagc
     1861 agttgataaa attgaagaag tgactgaaga aggtaagaaa ctttataaaa tcactgccga
     1921 agctcaagat ttgattcaac atacagaccc aacgaaggtt cgtaacaagt atgttcatta
     1981 tattgaaaaa ccagttccaa aagttgatga cgtttattat aactttaaag agttagtaga
     2041 tgccatgaat gctgataaaa atggtacatt taaaatcgga gcagatttga atgcaacgaa
     2101 tgtaccaaca cctaacaaac aatatgttcc aggtacgttt aagggacatc tatcaagtgt
     2161 tgatggtaaa caatatacaa ttcataacat tgcccgtccc ttatttgacc gtgttgaaaa
     2221 tggttctgtg aaaaatatta atttaggtaa tatggatatc aatatgcctt gggctgacgg
     2281 aatagcacct gttgccaata tggtgaaaaa tgcaactgtt gaagatgtta aggtaactgg
     2341 taatgtagtt gctaataaca atattgcggg tatcgtcaat aaaatcgata gtggtggtca
     2401 gttgacaaat gtagccttca tcggtaatct tacaggtgtt ggtgataaag gtcaatatat
     2461 ggctggcatt gccggtgaga tttggagagg taatcttgca aaagcatatg tcgaggctga
     2521 tattgttgct aacagagccc gtataggtgg tttagttgct aaaacagata atggtaacga
     2581 ctctatggga attggtaaat acggttcaat tcgtaaatca gtcaccaaag gaaccattaa
     2641 gactaaagtt ttgtttgaaa caggtggatt tatcaatagc aatcttcctt ttggtaaact
     2701 tgaagataat atttcaatga tgagagttga aaatggtgaa gagttctttg gttcttccga
     2761 tttggactac gatggtggtt atttcacaaa tggctggtta gagcgtaact ttgttgtcaa
     2821 aggtgtcagt tcaggtaagc attcatacaa acgttctcgt gacaaaatta aagaaattag
     2881 tcaagatgaa gctaataaga gaattgcaaa ttttggactt actgctgata aatacgaaat
     2941 caacgaacct gtggtcaatc gcttaaatcg tttaaccaga agagaagatg aatataagtc
     3001 aactcaagac tacaaatccg aacgtgattt agcttatcgt aatattgaga aacttcaacc
     3061 attttataat aaagaatgga ttgtcaacca aggtaacaaa cttgcagaag attcaaactt
     3121 agctaagaaa gaagtccttt ctgttacggg gatgaaggat ggtcaattcg tgaccgattt
     3181 atcagatatt gataagatta tggttcacta tgctgatggt actaaggaag aaatggatgt
     3241 tactaagaat actgattcta aagtccaaca agtgcgtgaa tatagtgtat ctggtcttgg
     3301 tgatgttgtt tatacaccga atatggttgt taaaaatcgt gataaattga ttgctgatgt
     3361 taaatctcaa ttatcaagtg tggaattgat ttcacaagaa gtaagagatt taatgagtag
     3421 acgtgataaa cctgcagaga atacggatga acgtaagaat ggttatatta aagatctata
     3481 cttagaagaa agctttgccg aagtgaagca aaatctagat aagttagtca aatccttggt
     3541 cgaaaatgaa gaccatcaat tgaatggtga tgaagctgcg ataaaatcac ttctcaagaa
     3601 agtggaaacc aataaagcta aaatcatgat ggctctgact tatctcaatc gttactatga
     3661 tatcaaatac ggtgacataa gtataaaaaa catcatgatg ttcaaacctg atttttatgg
     3721 taaaacacca agtgttattg acagactcat caacatcggt tcaagtgaaa agaacttgaa
     3781 aggggatcgg actcaggatg cttaccgtga aatcatcgca ggtaatactg gtaaaagtaa
     3841 tttgcgtaac ttcttagaat acaacatgcg tctgtttaca gaggataaag acatcaatga
     3901 ttggtttatc cactcagcta agaatgttta tgtttcagaa cctaagacaa caaatacaga
     3961 gttgaaagat aaacgtcatc gtgtgttcga tggtttagat aacggtgttc atggtcgtat
     4021 gattttacca cttctaacgc ttaaagatgc ccatatgttc ttaatttcaa catataacac
     4081 gatggcgtac agttcatttg agaaatatgg aaaacataca gaagaagctc gtaatgagtt
     4141 caaaactaag attgatgaag ttgcacatgc tcaacaaact tatttagatt tctggtccag
     4201 attagctctg cctaatgttc gtgatagact tcttaagagt caaaatatgg taccgacacc
     4261 agtttgggat aaccagacct acaatggttc tcctgtaggt cgtcgaggat ttgacagtaa
     4321 aggtaaccct atagctccaa ttcgtgaatt atatggtcca acatggcgac accatgatag
     4381 agactggcgt atgggcgcta tggcatcaat attccctaat ccaaataatg atgacaaggt
     4441 cttgtttatg gtgacggata tgattagccc atttggtatt tcagccttta ctcacgaaac
     4501 aacacacgta aacgatagaa tgctttactt cggtggtcat aagcatcgtc aaggtacaga
     4561 cgttgaagcc tatgctcaag gtatgttgca aacacctgac agctcaacga ctaatggtga
     4621 atacggcgca cttggaatta acatggctta ccatcgtcca aatgatggta accaatggta
     4681 caacccagat cctgacaaat taaagacacg tgatgacatt gaccgctaca tgagaaacta
     4741 caatgaagct atgatgttgc ttgatcatgt agaagctgat gctgtacttc ctaaaattaa
     4801 aggagacaat agtaagtggt tcaagaagat tgacaaagaa atgcgctcta agattcagta
     4861 taatgatctt ttaggtccaa accaatggga tagtatccgt gatttgaaag atgaagaaaa
     4921 ggtaatgact ttatctagcg tcaatgattt agtggataat aacttcatga caaaacatgg
     4981 aaatccaggt aatggacgtt atagaccaga agattttaca ccaaactcag cttacgtaaa
     5041 tgtcaatatg atggcaggaa tttacggtgg gaatacgagt caaggtgctc ctggttcatt
     5101 atcatttaag cacaatgctt tccgtatgtg gggctattat ggttatgaaa atggctttat
     5161 tagttatgta tcaaataaat acaaagcaga agcagataaa aacaaccacg gtttactaag
     5221 cgataagcta ataattaata aagtatcgaa aggaaacttt aatactcttg aagagtggaa
     5281 gagacattgg tacggggaag ttcttgctaa agctaagaaa ggttttgagg ctatcgatat
     5341 tgatggcgtc catatcagta actatgatga gttaaggcct ttatttgata aagcagttga
     5401 agaagatttg aagaaaccag atgacttctc tcacactgtt gctcttaaat ctaaggtctt
     5461 caaagccctt cttaaaaaca cagacggttt ctttaataaa ctgtttaaag aagacattta
     5521 aatgtttaga atatagaagg aacagttttt tgctccctct gaaaagtcat catttgatgg
     5581 cttttttcta tatagggtaa aagataggct aaaagatagg gtaaaaggct atcatcggac
     5641 aaaataaaga aggcatgata taatataaag tagatttcta tgtcataaaa caagaactgt
     5701 ttggacatca ttcatttgaa aactctctat gttcaaacta tagtaaaata aaatagggga
     5761 tctaaatcct tgctacgaaa ggaaaaaact caatggctac tattcaatgg tttcctggtc
     5821 acatgtctaa agcgcgtcga caggtgcagg aaaatttaaa atttgttgat tttgtgacga
     5881 ttttagtaga tgcacgcttg cctctatcta gtcaaaatcc tatgttgacc aagattgttg
     5941 gtgataaacc aaaactcttg attttaaaca aggccgactt ggctgatcca gcaatgacca
     6001 aggaatggcg tcagtatttt gaatcacaag gaatccagac gctagctatc aactccaaag
     6061 agcaagtgac tgtaaaagtt gtaacagatg cggccaagaa gctcatggct gataagattg
     6121 ctcgccagaa agaacgtggg attcagattg aaaccttgcg taccatgatt attgggattc
     6181 caaacgctgg taaatcaact ctgatgaacc gtttggctgg taaaaagatt gctgttgttg
     6241 gaaacaagcc aggggtcaca aaaggtcaac aatggcttaa aaccaataaa gatctggaaa
     6301 tcttggatac accggggatt ctctggccta agtttgagga tgaaactgtt gcacttaagt
     6361 tggcattgac tggagctatc aaggatcagt tgcttcctat ggatgaggtt accatttttg
     6421 gtatcaatta tttcaaagaa cattatccag aaaagctggc tgaacgcttc aaacaaatga
     6481 aaattgaaga agaagcgcct gtgattatta tggatatgac ccgcgccctc ggtttccgtg
     6541 atgactatga ccgtttttac agtctcttcg tgaaggaagt ccgtgatggc aaactcggta
     6601 actatacctt agatacattg gaagacctcg atggcaacga ttaaagaaat caaagaactc
     6661 cttgtgacag tcaaggagtt agaaagccct atttttttag agcttgaaaa ggataatcgc
     6721 tcaggagttc aaaaggaaat cagcaagcgt aaaagagcca ttcaagctga attagatgaa
     6781 aatttgcgct tggaatccat gctttcttat gaaaaagaac tttataagca aggattgacc
     6841 ttaattgcag gtattgatga ggttggtcgt ggtcctcttg ctggtcctgt agtcgctgcg
     6901 gccgttattt tacctaaaaa ttgtaagatt aaaggtctca acgacagcaa gaaaattcct
     6961 aaaaagaaac atctggagat tttccaagcc gttcaagacc aagccttgtc gattggaatt
     7021 ggtatcatag ataatcaggt catcgaccaa gtcaacatct atgaagcaac caaactagcc
     7081 atgcaagaag caatctccca gctcagccct caaccagagc accttttgat tgatgccatg
     7141 aaactggact tgcccatttc acaaacctcc attatcaaag gagatgccaa ctccctctct
     7201 atcgcagcag catctatagt agccaaggta acacgtgatg aattgatgaa agaatacgat
     7261 cagcagttct ctggctatga tttcgctact aatgcaggat atggcacagc taaacatctg
     7321 gaaggcctca caaaactagg agttacccca attcaccgaa ccagctttga acccgttaaa
     7381 tcactggttt taggtaaaaa agaaagttaa ttgaaaggaa ataccatgga ggaacagtcg
     7441 gaaatagtcc gttctaagaa agaattcgcc tttgcatcca gcactatact atcccaagtt
     7501 ggtcgaggaa tcattgtcgg cctcatcgtt ggaattatcg tcggatcctt tcgtttctta
     7561 attgaaaagg gcttccacct gatacaagga gtttatcaag atcaagggta cttagtgcgc
     7621 aatctttttg tactggtttt gttttatata ctcatctgtt ggctcagtgc caaactaaca
     7681 cggtcagaaa aagatattaa aggctcagga attcctcaag tcgaagccga actgaaaggc
     7741 ctcatgtccc tcaactggtg gggcattctt tggaaaaaat atgtgctagg tattcttgct
     7801 attgccagtg gactcatgct gggtcgagag ggacccagca ttcaacttgg agcagttggt
     7861 ggtaaaggaa ttgccaagtg gctcaaatcc agtccagtag aggaacgttc cttgattgcc
     7921 agtggagctg cagcaggttt agccgcagcc tttaatgctc ctattgcagc acttctcttt
     7981 gttgtagaag aagtctatca ccatttttcg cgctttttct gggtctcaac tctagcagcc
     8041 agcatcgtag caaactttgt gtctctactc atgttcggtt tgacaccagt attggatatg
     8101 ccagataaca ttcctcccat gaccctagac cagtattgga tatatctcgt catgggaatt
     8161 ttccttggat tttcaggttt tctctatgag aaagctgtat taaacgttgg aagagtttat
     8221 gacttgattg gtcaaaaaat ccatttggat agggcttatt atcccatctt ggcttttatc
     8281 cttatcatac cagtcggaat cttcttacct caaatcattg gtggcggaaa tcagcttgtc
     8341 ctttctttaa ctgaacaaaa ttttagtttc caagttttat tagcttactt tttaatccgc
     8401 tttatttgga gtatgattag ctatggaagt ggactgccag gaggaatttt cctccccatt
     8461 ttagctcttg gttctttgct tggtgcctta gttggtgtta tctgtgtcaa tcttggactt
     8521 gtcagtcaag agcaattccc tatatttgtc attctaggaa tgagtggcta ttttggagcc
     8581 atatcaaaag ctcccttaac cgctatgatc ctcgtaactg agatggtagg agatattcgc
     8641 aaccttatgc cacttggtct tgtcactctt gtttcttata ttatcatgga tttgctcaaa
     8701 ggtacgccag tctatgaagc catgctggaa aaaatgcttc cagaagaagt atctagcgaa
     8761 ggagaagtta cacttatcga aataccagtt tctgataaaa ttgctgggaa acaagttcat
     8821 gaactcaact taccacacaa catcctcatc acaactcaag tccataatgg caagagccaa
     8881 acagttaacg gctcaaccag aatgtatctg ggtgatatga ttcacctggt tattccaaaa
     8941 agtgaaattg gaaaagtcaa agatttgttg ttgtagtatg atcatttaca taatttatgt
     9001 tatgtaaatg atcggtttga tttatttaga aaaccgattc tcataaatga gatcgg
//
LOCUS       DAVMGZ010000083         9009 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000083 DAVMGZ010000000
VERSION     DAVMGZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.084"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(351..1199)
                     /gene="dprA"
                     /locus_tag="VYQ93_001488"
     CDS             complement(351..1199)
                     /gene="dprA"
                     /locus_tag="VYQ93_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV3336157.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1295..1984)
                     /locus_tag="VYQ93_001489"
     CDS             complement(1295..1984)
                     /locus_tag="VYQ93_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV3336158.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1996..2874)
                     /locus_tag="VYQ93_001490"
     CDS             complement(1996..2874)
                     /locus_tag="VYQ93_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV3336159.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2864..3733)
                     /gene="licA"
                     /locus_tag="VYQ93_001491"
     CDS             complement(2864..3733)
                     /gene="licA"
                     /locus_tag="VYQ93_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV3336160.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            complement(3750..4772)
                     /locus_tag="VYQ93_001492"
     CDS             complement(3750..4772)
                     /locus_tag="VYQ93_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV3336161.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4777..5484)
                     /locus_tag="VYQ93_001493"
     CDS             complement(4777..5484)
                     /locus_tag="VYQ93_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV3336162.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5820..7307
                     /gene="tacF"
                     /locus_tag="VYQ93_001494"
     CDS             5820..7307
                     /gene="tacF"
                     /locus_tag="VYQ93_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV3336163.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIIKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7317..8120
                     /locus_tag="VYQ93_001495"
     CDS             7317..8120
                     /locus_tag="VYQ93_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV3336164.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYELLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8122..8931
                     /locus_tag="VYQ93_001496"
     CDS             8122..8931
                     /locus_tag="VYQ93_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV3336165.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2829 a         1817 c         1518 g         2845 t
ORIGIN      
        1 tgttcacatc ttatttcaat tcactatact ttcccttata ctcaatgaaa atcaaagcgc
       61 aaactaggaa gctagccaca ggttgctcaa agcactgctt tgaggttgta gataagactg
      121 acgaagtcag ctcaaaatac tgttttgagg ttgcagatgg aagctgacgt agtttgaaga
      181 gattttcgaa gagtattata tgagaaaaat cctctactct actaatccac ttagaaaaaa
      241 cgcatattat caagtgttaa aaacttgcta atatgcgttt tattatggaa atgttgggtt
      301 cattttctct taaaattgat tttttcttag aattttagct taggtcattt ttaaaattca
      361 aattccgcaa gaacatcttg cccactggtg accaattttg ctccttcttg aatcaaatga
      421 tggcaaccgt ctgatagtcc atctaaaatg ctaccaggaa tagcaaagac atcgcgtcct
      481 tcttccattg ctcgctcaca cgtaatgaga ctacctgaac gcatcttagc ctctgctaca
      541 atcacaccac gacaaagtcc agcaatgatg cgattacggg caggaaaatg aaatttcaga
      601 ggttgttcgc caggtccata ttcacttaga accagatgat cattgccgat gtagtcttgc
      661 aagcgtttat tggctttagg ataaaacaca tccagtcctg ttccaatcac tgcaatggtt
      721 tttccgccat tctgaagagc tgccatatga gctgctgtgt caatgccctt ggccagacca
      781 ctgacaataa ccagttcatt ttccaagcct tgaatgactt tttcaactga cttagctccc
      841 tgtttgctac aagcacgact gcccacgacc gctaccttcg ggaatttcag gaggtcaaga
      901 tttcccttgt aaaataaaag tacaggcgca tcatatattt cactcaaatc ccaaggataa
      961 cagtcatcta aaatagagaa agatggaaat ttttgaaact ctttcgacaa atgcgcatcg
     1021 tctatctgaa aataacgttc cataaaaacg gctggattac ggcaacctga tatatctgca
     1081 atatcaccca acaaaagctc ctgatcaaca ttttcaccgt attctagcac tttcaaaatc
     1141 tgttgattgg tcaaacctga tttttttaac ttatagattt catagtttgt gattttcata
     1201 aataactcca tttctttttc tactcatcta tctattcgta aaagttcgta aaaaatcaaa
     1261 aaacatccga ctattttgtc agatgttgga atctttaatt ttcgttttta agaatttctt
     1321 ctaatttacg atagtcttgg acactatcga tctcataaat gctattgcct tctaattctt
     1381 caacatagac atctagctct ttgatattat ccttaaccat attgtcccaa tagagatcaa
     1441 caaattcacc acttgcataa gccttgtcga taaagctgac aatcttttct gcagttggag
     1501 catcccagaa ggatacacca ctaaggatgc gacctgcctt gctatcaaca ataatgtctt
     1561 gaaccttgta gtcatctcca tagaccaaga accattcgtt ggtacaatct tcacgataaa
     1621 cactaaaata agtcgaacgt gtcaaatcat tgcggaacat atttttaaag agataattgt
     1681 cagcatcaat aacatagctg ttggccaatt cttcttttac aagatagaga gagtaaaagt
     1741 tattgtagtc agcgtattta tcattgaaaa cgaggcgaac accgtatttt tcttttaagt
     1801 aatcgaattg ttctttaaga taaccaacaa tgatgatgat gtcattgatt cctttttctt
     1861 tgagaaactc aatttggtat tcaatcaaag gtttttgatt aacctgaacc aaggctttag
     1921 gggtattttc agtcatagga cgcaagcgag ttcccaatcc cgctgctaag ataatggctt
     1981 tcacacgaat ctcctttatt ctttaataat aatataaact ccagcaatga cgacaagtga
     2041 cgtcataatg gtcagcatat ctagcggtgc acccaaaaaa acaactgcaa acaagaccgt
     2101 ccatactaca tagctcacgt tcaagcctgt agccttggct ggttgcaagc gattgatagc
     2161 gatataataa gccaagtagg aaatcatatc aaaggctgca aaaacaatca tgagacctag
     2221 caattgtcca ttggctactg cagtaaatga ctgatgagag aagagcacaa tcacaagata
     2281 ggacaagaac gaagtcactt gacggattaa gagggcttcg atttcactca gttcactttc
     2341 catagcaaag gagctaagaa cactctcact tccccatgca atagcacaaa ccaaagcaca
     2401 gagaatccca atgtagaagg aattaacctg ttcaacctta taggtctggg caataatccc
     2461 tccaataatc aagacaatcc caaatacagt atttttcgaa atcttgtgct tcaaaaagaa
     2521 aaaagccaat agaactgaaa tcgcagggta aatagccgat acagatgaag ctaaagaact
     2581 tccgatatac ttaactgcat aaagattggc ctgcataccg atagggcctg ctagcaaggc
     2641 tccgatgata acactgacat tgcgaatatt taagaaaatt gagaggcgaa ctttcccttc
     2701 ttttaccaag agaaaagcta gtaagataaa gatgctcaaa aaatcatgag ctgcagccac
     2761 cacaaagggt gacaaatctg taaaaatcga aaagatataa gcactaaccg ttagacccaa
     2821 gccccagaaa atacctgaga gaaggccaaa aggaactccg tttttacttt tcatctgaac
     2881 ctccataaga agccaaacct ttaatagctc tttggtaacg attcacacca tagtcaccaa
     2941 aatcttcacc ttgctcttcc ttatagacag tccatagact ccaaatagta tcttgtaaaa
     3001 ttttataaat agcaatcttt tcatgagaaa ccggtgtttg gtcactctca tagtgagata
     3061 agaaagtttc ttcctcttgg gaagtgaatt cagactctaa aaagagggca gccaaatccc
     3121 acattggatc attcattgat gaatattccc agtcaatcaa ataaagtcgt ccttgaggtg
     3181 attcgataaa gttttcaggc accaaatcga tatgacaaga ttttctgtca acacctaagt
     3241 cagccagtct tttctctaag gagaagactg catttctaac agattcatag ttggcataag
     3301 gaatttgttc ttcaatcaag gattcgtatt ttttgatttc ttcaaaagga gcaaattctc
     3361 ctcttaattc cttagcagac gtatgaatag tttgtaatat tggagtaatt ttgtcgaact
     3421 tggtcttgat tgacgttgaa tcaagcgtaa tcgcagattc gatatactca tttactttga
     3481 taccagcttc aatatcaaaa agataatttt ttacatctaa gcctaaatcc tttagtagtt
     3541 caagattgta cttttcatct tgtcgattga taagcttttc tgtcccttta ccaaagaatt
     3601 taacaatgta ttgcttattt gttgttttgg ccaaatagtt ttgattggtc attccaccca
     3661 gttgttcaac actgaggact tcctcttctt gactaagtaa ggaagaaatt ttttctttaa
     3721 tgattttctc cacaattaac ctccagtact tatacttccc acttaaacac tgttttaaag
     3781 gctgtgttta aatcggttgc aaagacacga tgaatatctt taatttctct tacaggttct
     3841 tctagataaa ggatattttt aagacgatta gcaaatttct tgacttccat catttggata
     3901 gcattttcaa aatcaatgcg accagaacga gatgacccaa ccaaaatcaa gcccttttct
     3961 aaggcatcgc gagtattgag attgacttta tattcgctaa ctcccatcat gagaatcgtt
     4021 ccctgaggac gaatgtagcg aatcaagtca ttaatagctg gtccagtacc atcaccacca
     4081 caacattcaa aagcatggtc aaaggccaaa tcttcaggaa tattatccgt aatatagcat
     4141 tctttggcaa atgagaagag ttccaacttt tcccaatgac gaccaataac cacaatctct
     4201 gcttctggca aagtatagtt gataatattg gcaaccacaa aagctaaact tccatctcca
     4261 ataacggcga tccgctcccg cttgctatga gcaagagtca atagacgatt catagcgtgc
     4321 atgcccacac tgacaaactc tgtaatggct gcaaccgtat cttcaatagc atcataagcc
     4381 accacacgat ctttagggag agaaacaaac tctctcataa agccatcaaa tccactagac
     4441 aagaaatggg tccctgtcat gtagttttca tagaattctt catcactctg cataggagac
     4501 tgattgggaa tcatgacaac tttttgacca acctcgtagg ttccggtcgg gtcagaaatg
     4561 acggttccac atgactcgtg aatcattgcc attggaagct ttttattcaa aatcttggga
     4621 tcacgttttc cctgatagta acgctgatcc gcatgacaga cagccatgta gttgggacgg
     4681 ataaggatat gattctcttg gtcaatagcc tcttcctgat atttgacatt gataaactta
     4741 ggcttagtta gttgataaat ttgattaatc attttactag tctttctcaa tcatactttt
     4801 tgcaatcttc aaatctgtta cggttgtaat cttcagattt gagtattcac ctttggccaa
     4861 agccacatct tttcctttga tcacaaagat tttacatgca tctgtcaaga tttccttctc
     4921 ttcatcagaa agagatccat aaaggtccat gaagtccttg caacggaatg tttgaggtgt
     4981 ttgtccttga taaaggtgag cacgatttgg aatatctgta ataaattgac cattggtact
     5041 ttcaacgata gtatcaaccg cttctaccac tgtgtccact gcgtcatgat tttgggcaag
     5101 ttggatattg tcctgaatca tgcgaagtgt aataaatgga cgaacagaat cgtgggtaac
     5161 aacgatatcc tctggagtaa gcggacgata agcatcaatg gcttcaatga tgttcttaat
     5221 acttgtattg cggtcagcac caccctttgt aatgatgata cgttccttat aaagaggaag
     5281 atatttatct acaagatctt ctgcatgaga aacccagtct ccatgaacac caactacaat
     5341 tttttcaata cttggctcca agacaaattt ttcaattgta tgaatcaaaa taggtcgatc
     5401 acctagctct aaaaattgtt ttggcaagtt actgatcccc atgcgtgtgc cagttccacc
     5461 ggcaagaatt cctgcataaa tcatctattt tctcctttgt cttactaaaa aaacttattc
     5521 tctctattat accacaatct agtcctatca taaaagaaat actggtcctc cccttctaga
     5581 ataggagaat tatgcagttc tattattcaa agaaactagt ccatttctag taatgactag
     5641 gaattctgta gcaattacta ataaatggct gcgaaatttt agagtccttc agaataatat
     5701 actttcctta aagaaaactt aaaacttaag atttgaaact tcaaagtgat tacttgactt
     5761 actgcccttc atttcttatt agctaacttt atgatataat gaaaaacgag gtaaattgaa
     5821 tgaaaagtat aaaattaaat gctctatctt acatgggaat tcgtgtcttg aatattattt
     5881 ttcccatcct aactggaacc tatgtcgcgc gtgtcttgga ccgaactgac tatggttact
     5941 tcaactcagt cgacactatt ttgtcatttt tcttgccctt tgcaacttat ggtgtctata
     6001 actacggttt aagggctatc agtaatgtca aggataacaa aaaagatctt aacagaacct
     6061 tttctagtct tttttatttg tgcatcgctt gtacgatttt gaccactgct gtctatatcc
     6121 tagcctatcc tctcttcttt actgataatc caatcatcaa aaaggtctac cttgttatgg
     6181 ggattcaact cattgcccag attttttcaa tcgaatgggt caatgaagct ctggaaaatt
     6241 acagttttct cttttacaaa actgccttca tccgtatcct gatgctggtc tctattttct
     6301 tatttgttaa aaatgaacac gatattgttg tctatacact tgtgatgagt ttatcgacgc
     6361 tgattaacta cctgattagt tatttttgga ttaaaagaga catcaaactt gttaagattc
     6421 acctaagtga ttttaaacca ctctttctcc ctctgacagc catgttagtc tttgccaatg
     6481 ccaatatgct cttcactttt ttagatcgcc tcttcctcgt taaaacaggg attgatgtca
     6541 acgttagtta ctataccata gctcagcgaa ttgtgaccgt tatagctggg gttgtaacag
     6601 gtgcaattgg agtgagtgtg cctcgtctca gttactatct ggggaaagga gacaaagaag
     6661 cctatgtttc tctggttaat agaggtagtc gaatctttaa cttctttatc attccactga
     6721 gttttggact catggtttta ggaccaaatg ccatcctact ttacggtagt gaaaaatata
     6781 tcggaggcgg catcttgacc tctctcttcg cttttcgtac gattatcctg gccttagata
     6841 ccattcttgg ttcccaaatt ctctttacca atggctatga aaaacgtatc acagtttata
     6901 cagtctttgc tgggctactc aatttgggct tgaatagtct cctctttttc aaccgtatcg
     6961 tggctcccga atactactta ctgacaacta tgttatcaga ggcttctcta cttgttttct
     7021 atatcatttt catccataga aaacaactca tccacttggg acatatcttt agctatactg
     7081 ttcgatactc tctcttttca ctttcctttg tagcaattta tttcctgatt aatttcgtgt
     7141 atcctgtgga tatggtcatt aatttgccat ttttgattaa tactggtttg attatcttgc
     7201 tatcagctat ctcttatatt agtctacttg tcttcacaaa agatagcatt ttctatgaat
     7261 ttttaaacca tgttctagcc ttaaaaaata aatttaaaaa atcataggag tttaaaatga
     7321 aacaactaac cgttgaagat gccaaacaaa ttgaattaga aattttggat tatattgata
     7381 ctctctgtaa aaagcacaat atcaactata ttattaacta cggtactctg attggggcgg
     7441 ttcgacatga gggctttatc ccttgggacg acgatattga tttatccatg cctagagaag
     7501 actaccaacg atttattaac atttttcaaa aggaaaaaag caagtatgaa ctcctatcct
     7561 tagaaactga taagaactac tttaacaact ttatcaagat aaccgacagt acgactaaaa
     7621 ttattgatac tcgaaataca aaaacctatg agtctggtat ctttatcgat attttcccta
     7681 tggatcgctt tgatgatcct aaggtcattg atacttgtta taaactggaa agcttcaaac
     7741 tgctgtcttt cagtaaacat aaaaatattg tctataagga tagcctttta aaagattgga
     7801 tacgaacagc cttttggttg ctccttcgac cagtttctcc tcgttatttt gcaaataaaa
     7861 tcgagaaaga aattcaaaaa tatagtcgtg aaaatgggca gtatatggct tttatccctt
     7921 caaaatttaa ggaaaaggaa gtcttcccaa gtggtacctt tgataaaaca atcgatttac
     7981 cctttgagaa tttaagcctt cctgcacctg aaaaatttga tactattttg acacaatttt
     8041 atggagatta tatgacccta ccaccagaag aaaaacgctt ctacagtcat gaatttcacg
     8101 cttataaatt ggaggattag gatgcaatat ttagaaaaaa aagaaattaa agaaattcaa
     8161 ctagccctgc tggactatat tgatgagact tgtaagaaac atgatattcc ttattttctc
     8221 agttatggaa ccatgcttgg agccatccgc cacaaaggta tgattccttg ggatgatgat
     8281 attgatattt ccctttatcg tgaggattat gagcgtttac tgaagattat tgaagaagaa
     8341 aatcaccctc gctacaaggt tctttcctac gatacatctt cttggtactt ccataatttc
     8401 gcatcgattt tggacacttc tactgttata gaagaccatg ttaagtacaa gcgtcatgat
     8461 accagccttt tcatcgatgt cttcccaatt gatcgattta cagacttgag cattgtcgac
     8521 aagagctata agtatgtggc tcttcgtcaa ctagcttata tcaaaaaatc acgagcagtt
     8581 cacggtgata gcaaactaaa agattttctt agattatgta gctggtacgc tctccgattt
     8641 gtcaatcctc gctactttta caagaaaatt gatcaactag tcaaaaatgc tgtaaccaac
     8701 attcctcaat atgaaggagg agttgggatc ggtaaggaag ggatgaaaga aatcttccca
     8761 gttgatacct ttaaagaact gattttaact gagtttgagg gccgtatgtt gcctgttccc
     8821 aaaaaatatg accaattttt aacccagatg tatggcgatt atatgacacc accatcaaaa
     8881 gaaatgcaag agtggtatag tcatagcatt aaagcttatc gcaaaaactg attgaggggg
     8941 attatacaaa ctactaagat agaggttatt caaaaacata attttagtag aaaatgaaat
     9001 acatattcc
//
LOCUS       DAVMGZ010000084         8798 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000084 DAVMGZ010000000
VERSION     DAVMGZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.085"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            30..185
                     /locus_tag="VYQ93_001497"
                     /pseudo
     CDS             30..185
                     /locus_tag="VYQ93_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            385..3660
                     /gene="rexB"
                     /locus_tag="VYQ93_001498"
     CDS             385..3660
                     /gene="rexB"
                     /locus_tag="VYQ93_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV3336166.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGKQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3657..7307
                     /gene="addA"
                     /locus_tag="VYQ93_001499"
     CDS             3657..7307
                     /gene="addA"
                     /locus_tag="VYQ93_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV3336167.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7522..8700
                     /locus_tag="VYQ93_001500"
     CDS             7522..8700
                     /locus_tag="VYQ93_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336168.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLLLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2714 a         1849 c         1875 g         2360 t
ORIGIN      
        1 atttactata gttcaatcat tctatcaaga atattgaata tataaaggca cgtaatatta
       61 aaacttcttc tgatatcgaa ttagagtccg atgttgatgg cgataaatca gacaatctac
      121 cagtcgacat taagatactg ggacaacaca ttgaagtgta ttccgtacct aagaaacaat
      181 cataaaactc aaagccttgt taccgaggct tttttattat gtgattttca gtagccagac
      241 ttattctatc taatacaagt aaagctaagt atttaagatt tagcagatga tgaagaaaag
      301 aaaggatggc ctaaatattt taacagattt acgaggctaa tcatggtata atatagggtg
      361 atgaaaaatt ccaacgaggc tgagatgaaa ttactttata ctgatattcg gacttctttg
      421 acagaaattc taacaagaga ggcagaagag ctagttgcag ctggcaagcg ggtcttctac
      481 attgccccca actctctttc ttttgaaaag gaacgcgccg tgctggaatg cttgtcccag
      541 caggcttctt tttcgattac cgtcacgcgc tttgctcaaa tggctcgtta tctggtcttg
      601 aatgatttac cagctaaaac tactcttgat gatatcggtc ttgggttggc cttttacaaa
      661 tgccttgccg aactcgatcc caaggacttg cgtgtttatg gcgctattaa gcaggatcct
      721 caattgatcc agcagttaat tgagctttac catgagatga ccaaatccca gatgagtttt
      781 ttggacttgg agaatttaac agatgaggat aagagggcgg atttactctt gatttttgag
      841 aaagtaacag cctatcttaa tcaaggtcag ttagcccagg gaagtcagtt gtcccatttg
      901 attgaggcta ttgagaatga caaggtaagt agtgatttta atcaaatcac cttggtcatt
      961 gacggcttta ctcgtttttc tgctgaggaa gagcgggttg tggacttact tcacggcaaa
     1021 ggtgttgaga ttgttatcgg ggcttatgct agtaagaaag cctataccag tccttttagc
     1081 gagggcaatc tctaccaagc cagcgtaaaa tttctccatc atctggcttc taaataccaa
     1141 acgcctgctc aggactgttc tcaaactcat gagaagatgg atagttttga caaggcctct
     1201 cgtttgttgg agtcttctta tgacttttca gaactcgctt tggatgtcga tgagaaagac
     1261 cgtgaaaatt tacaaatctg gtcttgtttg acgcaaaagg aggagttgga gctagtagcc
     1321 cgtagtattc gtcagaaatt acatgagaac tcagacctga gctacaagca ttttcgtatt
     1381 ctcttggggg atgtagcttc ttaccagtta tctctcaaaa ccatttttga ccagtatcag
     1441 attccttttt atcttggtag aagcgaagcc atggctcatc atcccttgac tcagtttgtc
     1501 gagtctattt tagctttaaa acgttaccgt tttcgtcagg aggatttgat taatcttctt
     1561 agaactgatt tgtatactga cctcagtcag tctgatattg atgcttttga gcaatatatc
     1621 cgctatcttg gtatcaatgg cttgccagcc tttcagcaaa tcttcaccaa atcccaccat
     1681 ggaaaattta atcttgagcg tttgaatgtc ctccgcctga gaattttagc acctcttgaa
     1741 accctctttg ccagccgaaa acaaaaggct gaaaacctcc tacaaaaatg gagtgtcttt
     1801 ctaaaagaag gagctgtgac caagcagtta caagatttga caaccacttt ggaagctgta
     1861 gaacaggaaa gacaagccga agtttggaag gctttctgcc atgttttaga acaatttgcg
     1921 actgtttttg ctggttcaca ggttagtctg gaagacttcc tagccttgct ccattctgga
     1981 atgagtttgt cccaataccg taccattcca gcaacagtgg acactgttct ggtgcagagt
     2041 tacgatttga ttgcaccatt gactgctgac tttgtctatg ctattggact aactcaggac
     2101 aatttaccaa aaatttctca aaacaccagt cttctgacag atgaagaaag gcaaaaccta
     2161 aaccaagcga ccgaagaagg cgttcaatta ctgattgcca gcagtgaaaa tctcaagaaa
     2221 aatcgctaca ctatgctttc cttggtcaat tctgctcgta agcagttgtt cttgtcggct
     2281 ccaagccttt ttaacgaaag tgaaagtaag gaatctgcct atcttcaaga gttgatccat
     2341 tttggattta ggcggagaga gaagaggatg aatcacaaag gactgtctaa ggaggatatg
     2401 gggtcctatc acagtctttt gtctagtctg gttgcctatc accagcaggg tgagatgagc
     2461 gatactgagc aagatttgac ttttgtcaag gttctgtcgc gtgtcatagg taaaaaacta
     2521 gatctgcaag gtctggaaaa tccagctatc ccaaccagtc caagcagcaa gaccttagcc
     2581 aaggacacct tgcaagctct ctatcctgcc aaacaggagt tttacctgtc tacgtcgggt
     2641 ttgacagagt tttatcgcaa tgaatacagt tatttcctac gctacgtttt aggcttgcag
     2701 gaggaattac gtttgcgtcc tgatgcccgt agtcacggga atttcttgca tcgtatcttt
     2761 gaacgcgcct tacagttgcc taatgaagat tcctttgacc aacgtctaga acaagctatt
     2821 caagaaacca gtcaagaacg cgaatttgaa gctatttatc aagaaagttt ggaagcccag
     2881 tttaccaagg aagttttgct tgatgttgca cggacgactg gacatattct ccgacacaat
     2941 ccagccatcg aaaccatcaa agaagaagca aattttggtg gaaaagacca agcctttatt
     3001 caattagaca atggacgcag tgtctttgta cgaggcaagg tggaccggat tgaccgtttg
     3061 aaagctaatg gagcgatagg agtagtagac tacaaatcca gtctgactca gttccagttt
     3121 cctcatttct ttaatgggct caattctcag ttaccaacct atcttgctgc cctaaaaaga
     3181 gaagggaagc agaacttttt cggcgccatg tacttggaaa tggctgaacc tgtccaatct
     3241 ctgatggcgg taaaaagtct ggcaggagca gtggtagaag ccagcaaatc tatgaaatac
     3301 caagggctct tcttggaaaa agaaagcagt tatttaggcg aattttataa caaaaacaag
     3361 gctaatcaac tgacagatga ggaatttcag ctcctactgg actacaatgc ctatctttac
     3421 aagaaagctg ctgagaagat tttagcaggc cggttcgcca tcaatcctta tactgaaaat
     3481 ggcagaagca ttgccccata cgtccagcaa catcaggcta ttacaggctt tgaagccaat
     3541 taccatctgg gccaagcccg tttcctagaa aaattggacc tagctgatgg caagcgtctg
     3601 gtcggagaaa aactcaagca agcttggttt gaaaaaataa gagaggagtt aaatcgatga
     3661 agcttattcc ctttttaagt gaggaggaga ttcaaaaact gcaagaagca gaagcaaatt
     3721 cgagcaagga acagaagaaa actgccgaac aaatcgaagc tatctacact tctggtcaga
     3781 atatcctggt ctcagcatcg gctggttctg gaaagacctt tgtcatggca gagcgcattc
     3841 tggaccaatt ggcgcgtggt gtcgaaattt ctcaactctt tatctcaacc tttaccgtca
     3901 aggctgcaac tgaacttaaa gaacgtttag agaaaaaaat cagcaagaaa atccaagaaa
     3961 cagatgatgt cgatctcaaa caacacttgg gtcgccagtt ggcagaccta cccaacgctg
     4021 ccatcggaac catggactct ttcacacaaa aattccttgg caaacatggt tatctgcttg
     4081 atattgcacc taatttccgt attttacaaa accaaagcga gcaacttctt ctcgaaaacg
     4141 aagtctttca tgaggtcttt gaagcgcatt accaaggtaa acagaaagag agctttagtc
     4201 atttgctgaa aaactttgct gggcgtggca aggacgaacg gggtctgcgg cagcaagtct
     4261 ataaaatcta tgacttcctc caatccacca gtaatcctca aaagtggctg agtgaatctt
     4321 tcctcaaagg ctttgagaaa gctgatttta ccagtgaaaa agaaaaactg actgagcaaa
     4381 tcaaacaagc cctctgggat ttggaaagct ttttccgtta ccatctggat aacgatgcca
     4441 aggagtttgc aaaggctgcc tatttagaaa atgttcagtt aattctggat gaaattggct
     4501 ccctaaatca ggagttcgat agtcaggctt atcaggcagt acttgcgcgt gttgtcgcca
     4561 tctctaagga gaaaaacggt cgtgctctga ctaatgccag ccgtaaggct gatttgaagc
     4621 ccctggctga tgcctacaac gaagagagaa agacccagtt tactaaacta ggacaattat
     4681 cagaccagat agcgattctc gactatcaag aacgttatca tggagacact tggaaactag
     4741 ctaaaacctt ccaatctttc atgagcgatt ttgtagaggc ttatcgtcag agaaaacgac
     4801 aggaaaatgc ctttgaattc gctgatatca gccattacac cattgagatt ttagaaaatt
     4861 tcccacaagt tcgtgagtct tatcaggagc gcttccatga agtcatggtc gatgagtatc
     4921 aggataccaa ccatattcaa gaacggatgc tggaattgtt gtctaatggc cacaatcgct
     4981 ttatggtggg agatatcaag caatccatct atcgtttccg tcaggcagac ccgcagattt
     5041 tcaatgagaa attccaacgc tatgcgcaaa atccccaaga aggcaagctc attctcctca
     5101 aggaaaattt ccgtagtagt tcagaagtgc tgtcagcaac caatgatgtc tttgaacgtc
     5161 tcatggacca agaggtcggc gaaatcaact atgataacaa gcaccagctt gtttttgcca
     5221 ataccaaact gactcccaat ccagacaaca aggcagaatt tctcctctac gacaaggacg
     5281 atacaggtga ggaagaagag agtcaaacag aaacgaaact aacaggcgaa atgcgcttag
     5341 ttatcaagga gattctgaaa cttcatcaag aaaaaggtgt tgcctttaag gaaattgccc
     5401 ttctgacctc cagccgcagt cgtaatgacc agattctcct cgccctgtct gagtacggga
     5461 ttcctgtcaa aactgacgga gagcaaaaca attatctcca atccctagaa gtgcaagtca
     5521 tgctagacac tcttcgtgtc attcacaatc ccctgcaaga ctatgccttg gttgccctta
     5581 tgaagtctcc aatgtttggt tttgatgagg atgagctagc acgtttgtcc cttcagaaag
     5641 cagaggataa agtccacgaa aatctctatg agaaactggt caatgcacaa aaaatggcaa
     5701 gtagtcaaaa aggcttgatt cacacagctc tagctgaaaa actaaagcaa ttcatggata
     5761 tcctagcttc ttggcgcttg tatgccaaaa cccactctct ctatgacttg atttggaaga
     5821 tttacaacga ccgtttttat tatgactatg ttggggcttt gccgaatggt cctgctaggc
     5881 aggccaatct ctatgcccta gcactgcgtg ctgatcaatt tgaaaagagc aatttcaaag
     5941 gtttgtcgcg ttttattcgt atgattgacc aagtcttaga agcccagcac gatttggcaa
     6001 gcgtggccgt cgcaccgcca aaagatgcag tagagctcat gaccatccac aagagtaaag
     6061 ggctggagtt tccttacgtc tttatcctca atatggatca agatttcaac aagcaagact
     6121 ctatgtcaga agtcattctc agtcgtcaga atggtcttgg tgtcaaatat attgccaaga
     6181 tggagacagg ggcagtagaa gaccactatc ctaaaaccat caaactctcc attcctagtc
     6241 tgacctatag gcagaacgaa gaggaattac agctagcaag ctattctgag cagatgcgtt
     6301 tgctgtatgt tgctatgacg cgggctgaga aaaagctcta tcttgtcggc aagggttctc
     6361 gtgaaaagct ggaagtcaag caatacccag cagccaaaaa tgggaaacta aatagcaata
     6421 ctagactgca agcacggaat ttccaagatt ggctttgggc tatcagtaaa gtgtttacta
     6481 aggacaagct caactttagt tatcgtttta ttggcgaaga tcagttgacc agagaagcta
     6541 tcggagagtt ggaaaccaag agtcctctcc aagatagctc ccaagcagac aatcgtcagt
     6601 cagataccat caaagaagct ctggaaatgc tgaaggaggt ggaagtttat aatactcttc
     6661 accgcgcagc tattgaactt cctagtgttc aaaccccaag tcaaatcaag aaattctacg
     6721 aaccagttat ggatatggaa ggtgtcgaga ttgctggtca aggtcagtca gtaggcaaga
     6781 aaatcagctt cgatttgcca gatttttcaa ccaaagaaaa ggtaactgga gctgagattg
     6841 gtagtgctac tcacgaactc atgcagagaa ttgacctcag ccagcaacta acccttgcta
     6901 gcctaacaga aacactcaaa caagttcaaa ctagccaagc tgtcagagac aagatcaatc
     6961 ttgataaaat tcttgctttc tttgacacag tactcggtca ggaaattctt gctaataccg
     7021 accatctcta tcgcgagcaa cctttctcca tgctcaaacg agaccaaaag agtcaggaag
     7081 actttgttgt ccgtggtatc cttgatggct atctgcttta cgaaaacaaa attgttctgt
     7141 tcgactacaa gacagaccgc tatgatgaac caagtcaact cgtagaccgc tatcgtggtc
     7201 agttagctct atacgaagag gctttatcac gagcctattc gattgaaaat attgaaaaat
     7261 acttgatttt actcggtaaa gacgaggttc aagttgtaaa agtataactt atactctttg
     7321 aaaatctctt caaaccacgt cagcttcgcc ttgctgtata tatggttact gacttcgtca
     7381 gttctatcca caacctcaaa acagtgtttt gagctgactt cgtcagttct atctacaacc
     7441 tcaaaacagt gttttgagct gattgcggct agctttctag tttgctcttt gatttttatt
     7501 gagtattaga aaggagacct catgacactt ccagttagaa aatccctgca cgatgcagtt
     7561 ttacaggctt cgaaagcgga tacttgggaa caagctacca aggaatggaa tgaagtttcc
     7621 ttgattttta acggtatcgg ccgtagcaat tgtgtctgtg gaaatgccat caaatacgct
     7681 tatgaactct ttaacggtgt cacaggccaa cgcctctttc caattggtag cgactgtgtt
     7741 cgtcattttc atcgattgtc ccttgaccag caattggaag aggaagaaaa actgctcaga
     7801 aaggttgaaa atctaaccag aaaagctcag aaaaaggaaa aaatcaaggt caataaaagt
     7861 gactttgacg agcgacttct aaaatggctc tgggaaaaag gtgttttcaa acccaatcgt
     7921 ggcaatcaat ttgcgcctga gagagactac cagcttttac tagaagtctt tcagggagga
     7981 agttggacca aggcggaacc aaagaaaaag gctcggatgg aagaagtcct tgaaaagtgt
     8041 atcaaaccct ttttacttgg gaaatcggat gaccaactct accttgtcaa gctaggcaag
     8101 gagaaaattg actacgaaca agaactccgt atccaggcag agaaagaacg caagaagagg
     8161 gataaaatcg ccaagcaata tgcagacaat ctggttctag ccatgggacc cgcagagcgt
     8221 gcttatcaag attactttgg ctttacagag accctgactc aagaagaacg caagtgggaa
     8281 aaaattcttt ttggtaaaaa tagagcagaa cgggctatca aggccaaaca ataccaaaaa
     8341 gagctggaaa aagaccaacg aattgccagt caggatccaa ttgaaagaaa acagaagcag
     8401 acttggctcc tcaattccta ttttcgtgag cttcctgaag aaaaagctcg attttctagg
     8461 ctcttattag aatatcgaaa aagtggtgaa gtaccctttt caactgaata cctgtccgat
     8521 catctcatcg actttttcta taaaatgaaa gcctttgaat ttgaaatcgc accagaacaa
     8581 gtccgagatt ttctaaaaaa aagcctgcag gaagaccatc gctcatcagc acaaggaagc
     8641 tggatagaag gaattctcct caactgcctc aaaccatttt tagaacgatt agtgatatga
     8701 gtaacaccta ccgtgtaaat ttcatggtag gtgttattgt acccccaaat aatataaaac
     8761 aaagtatgaa agcgcatctc aattttaaga cttttttg
//
LOCUS       DAVMGZ010000085         8732 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000085 DAVMGZ010000000
VERSION     DAVMGZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.086"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..399
                     /locus_tag="VYQ93_001501"
     CDS             <1..399
                     /locus_tag="VYQ93_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV3336169.1"
                     /translation="SEIIAYNLSTSPNLEQVQTMLEQAFKEKHYENTILHSDQGWQYQ
                     HDSYHRFLESKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKSFQSLKQLEQAI
                     VDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(527..730)
                     /locus_tag="VYQ93_001502"
     CDS             complement(527..730)
                     /locus_tag="VYQ93_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3336170.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(723..1805)
                     /locus_tag="VYQ93_001503"
     CDS             complement(723..1805)
                     /locus_tag="VYQ93_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV3336171.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1931..2713)
                     /locus_tag="VYQ93_001504"
     CDS             complement(1931..2713)
                     /locus_tag="VYQ93_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV3336172.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2713..4056)
                     /locus_tag="VYQ93_001505"
     CDS             complement(2713..4056)
                     /locus_tag="VYQ93_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV3336173.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4155..5471)
                     /locus_tag="VYQ93_001506"
     CDS             complement(4155..5471)
                     /locus_tag="VYQ93_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV3336174.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5664..6890
                     /locus_tag="VYQ93_001507"
     CDS             5664..6890
                     /locus_tag="VYQ93_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV3336175.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            7089..8663
                     /locus_tag="VYQ93_001508"
     CDS             7089..8663
                     /locus_tag="VYQ93_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3336176.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRIEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2507 a         1693 c         1939 g         2593 t
ORIGIN      
        1 agcgaaatta tcgcctataa tctttcaact tcacccaact tagaacaagt acaaacaatg
       61 ttggaacagg cattcaaaga gaagcactac gagaatacga ttctccatag tgaccaaggc
      121 tggcaatatc aacacgattc ttatcatcgg ttcctagaga gtaagggaat tcaagcatct
      181 atgtcacgta aaggtaacag cccagacaac ggtatgatgg agtccttctt tggcattctg
      241 aaatcggaga tgttttatgg ttatgagaag tcatttcagt cgcttaagca attggaacaa
      301 gccattgtag actatattga ttactacaac aataagagaa ttaaggtaaa actaaaagga
      361 cttagccctg tgcaatacag aactaaatcc ttcggataaa ttaattgtct aacttttggg
      421 gggcagtaca ttttaacact tgatactatg cgttttattg tgggaagatt ttcctcattt
      481 tctagtgaaa ttgaactttt tctaacatct tttgatagat gacttcttaa tcaattatca
      541 tttccattgt tgtttcttgg ggtttcatcc cttgtctttg ataaaagcgg agagcaccct
      601 cgttatcatt ccagacactt agggttagat tattgcagcc aatttcttta gcataatagc
      661 agaaaaatga caataaaata gcccctagat attttgaaag gaaagcatag gggctatata
      721 gtttaatcaa aataaagcaa atctttgctg gtgcttgtaa agaggtcgaa gccatccttg
      781 gtaacaacac cgcagtcttc aatacgaaca ccgactttac cagggatata gatacctggt
      841 tcaacagaga agcacatgcc ttcttcgatg accatgtcgt ttccttccat gatagatggg
      901 aattcatgga catccatacc gataccatgc ccgagacggt ggttgaagta ctcaccataa
      961 ccagcttttt cgatgacctc acgggcagcg cggtccactt catgagcagt cacacctggc
     1021 ttgataaagt caagagcagc ttgttgggct tcaagagtca agttgtaaat atctttcttg
     1081 aattggtctg gtttgccgac agcgactgta cgagtcatat ctgacgcata gccattgacc
     1141 agaacaccca ggtcaaagag gagaagagca tcattttcaa ccttattagc tgctggaatg
     1201 ccgtgtggat tcgcagcatt atcaccagtc aagaccatgg tatcaaagct catttcataa
     1261 ccttcacgtt tcatggcaaa gtcgatttgt gcgatgatat ctgtctcagt cttatcaaga
     1321 gaaatattgt caaaaccaac atgaacagcc ttgtcagcat aaagacctgc aaccatcatt
     1381 ttttgcactt catcagctga tttgatgagg cgcatgcgtt ggatacgagg agtgaggttg
     1441 tcaaactcag cagtctcaaa aactgttttc aaaccatggt atttggtcaa gatgagattg
     1501 tcaaactcaa cagcgacacg tttgaagtca agttgtggaa gagcatgttt gattttttgc
     1561 catggatttt cagaatcgac atagcccact actgggaagg aaacggtgct acttgcacgt
     1621 tctacttcaa gagctgggac aaagaggaga ggttcctgat ccgctaggac aaagaggaac
     1681 atttggcgtt catggggatc actgtaaaaa ccagtgaggt aattgattgt gacggggtca
     1741 gatacgacag cgacgtctag tttttctgat tcaagatatg ttaggatttg ttgtaattta
     1801 gacatatgct accttctttc tacccctcta ttttggcaaa aaatgagaga aaatgcaagg
     1861 gagaagggta aaagaaaaga caaaaaactc cgacaagata acggagttct ttgatatcat
     1921 tttcctttgt ttaagaaaag tcagccttgc ttttgacgtc actgtactct tcagcgattt
     1981 cttggctgag aatgtcctca taggcaggga gttggatgtc ctgaccatat ttcttcttga
     2041 gggcagtagt aactaggcga taagccagat tggcattacg agagaggata ggcccgtgga
     2101 agtaggaacc aaagacattc ttataatgaa ccccttcacc gaccttttct tcgttgtttc
     2161 catttccata gacaacctgt cccagcggtt tttggtcatc agagaggaag gtacgacctt
     2221 ggtgattttc aaatccatag taggtttcat cgaaatcttc attgtgaatc ttgatgtcac
     2281 cgataaaacg gttattggtc tggttgagcg tgtagtgtcc catgacccct agcccttcga
     2341 tacgttttcc tgaagcttca acataatatt gacccaatag ttggaaacca ccgcagatag
     2401 ccagaactac accgtcgttt tggatgtagt tgtcaatgct ctctttttta gcaggtaggt
     2461 cgtctgcaat gatactttgt tcaaagtctt gaccaccacc gaaaaaggcg atgtcgtagt
     2521 gattttcatc aaagtcatca tggagagaaa cgatgtcaac ggtcacatgg gctcccagtt
     2581 tttcagccac atacttgagc atgaggatgt ttccattgtc cccgtaggta ttcatgagat
     2641 ttccgtagag gtgggcaatg ttgagctgat aggggtaatt gctatctttt gaggaaagtg
     2701 aagtataaac cattagttca tctcctttct aacaatctga cgactagcca gcagttcacg
     2761 aaattccagc atggcagtat aagttgccag aatataggca tgcttgcagt cttgattctc
     2821 aatggtcttg agaacttgct ccagattact cgtttcagtg attttctcag ctggatagcc
     2881 agtcactcgg aggcgacgag cgatttcaga atgacgaaca ccgccagcgt tgatttcagg
     2941 aatgtccatg tcagtgattt gctcaaagtc tgcatcccag atccagctag tgtcaattcc
     3001 atctgcatag ttggcattaa ggaggacaga taggctaaat ggataaggtg ctagtttgat
     3061 catttcgata gcttgggttg caccgactgg atttttaatc aagacaaggg tacattcctt
     3121 gtcaccgata tgaaaggttt cttggcgtcc aaagacagca cggctcttgt caaatccctg
     3181 tttgatgagt tgggaatctg cgccgaggaa acgggcgatg gccacagcag ctagggcgtt
     3241 atagatatta tagagcccgc cgatttggat accgtattct tggccgtcta tgacaaagcg
     3301 agagcgattg ttggtcaact caaccagttt tgtcaaacga tagtcgagat caggacgttt
     3361 acatccacaa ccttcacaga tataggcacc caagtttgca taggtattat gctcatattt
     3421 gaggatgcct tggcagtcag gacagagaat cccttcggta ttgtagtgag caagttgggc
     3481 tggtcctttt tccaagtcaa aaccaaaata ctctataggg tttggaatag ttggcttgta
     3541 gaaaagtgga ctgtctccgt taaggagaac agtagcagtt ggaacttttc gaatggcatc
     3601 caatatcatg ttataggtag tatagatttc accgaaacgg tccatctggt cacggaagat
     3661 attagtaatg acaaaaagac taggctggat atagtcacag atacgagata gactggcttc
     3721 gtcaatttcg aggacggcaa tatttttccc agtttttgaa gatttggctg ttaggaaggt
     3781 tgttgcaatc cctgtaatca tgttggcacc gcttgggttg gttagaactt gaccataaac
     3841 ctcttttaaa atgccgacag tgagggcagt tgtcagggtt tttccatttg ttccagtgac
     3901 aacgacaatc tcgtagttct tagctaggct ttgtaaaata tctttatcaa attgaagggc
     3961 gactttccct gggagcgtac ttccacgtcc aagacggctt aaaacgaagt gggaagaacg
     4021 cccagcaaga aggcccaaag tagtttttaa gttcatgttt ctattatatc acaaaagagg
     4081 gaaaagacag atttggtgtt tcgtagaagt aaaaacagcc caaagagggc tgtcagttaa
     4141 gatgagattt caacttaaat cgcaaacaag tcattcaagt tctcagccaa ggttgggtga
     4201 gtgaagattt gttttgtgaa gtaagtgtaa ggaatcttgt tgtccatagc aacagtaatg
     4261 atgttgatga tttcttgaga accttctgag aagatgcttg caccaagaat ttcttttgtt
     4321 tcagtattaa caacagcttt gaaagctccg cgaaggtctc catttacgtg accacgaggc
     4381 atggctgcaa cagggatttc tttcactgcg tatggaagtt tcaaatcagc tgcttggctt
     4441 tcagtcaaac caacttgtga aagtgcaggt gtgatgaaca tagtatttgg cacattgaga
     4501 cggtcctcaa gtgtgtagct gccatctcca gcaaggtagc tgtaaacaac acggaagtca
     4561 tcaagtgaaa tgtaagtaaa ttgaaggcca ccgttgacat ctccaactgc aaagacacca
     4621 ggaacgtttg tttgacagtg tttgtctact ttaatagcac cacgttcagt tagttcaata
     4681 tctgtatttt caagttgaag tggttctaca tttggtttgc gtccagttgc gtagagaagg
     4741 gcgtcgaaac ggtaagtttc gtcttcagtt acgacaagca cttggtcacc atcgtttttg
     4801 atttcagtag tatggatatt ttgaagcaat tcaatgccat cttcttccat gtattgttta
     4861 gcaagagctg cgatggaagg ttctgcacga ggtaggaatg tatccaaggc atctaggact
     4921 gtgaccttgc ttccaagttt gttgtaaagg ccggcaaatt caagaccgat atttccgcca
     4981 ccaaggattc caagtttttc aggtaatttg tccaagcttt ggatacctgt tgagtcaaag
     5041 atgtttttgc ttgtagcaag tccaggaatt ggcaagacgt ttgaaacagc accagtgttg
     5101 atgacgattg tttcagcagt cagttctttc ttttcatcac cagcttggat ttcgatgact
     5161 ttatttgaaa ggaagtgagc ttccgcatca aagatatcta cgcctgtacc agcaacagtc
     5221 gcatagtttt taccgttgag gcgaccagtg atcgtgtttt tagtagcaat cacttcttca
     5281 aaagacaagt ccttttcagc agcaactagc aaggttttag ttgggataca accaatgttg
     5341 atacaagttc caccgtacat agccttgctg cgttcaacga gggcaacttt tttgccagct
     5401 gaagccaatt tacctgctag tgttttacca gctttaccaa atccaataac aattaaatcg
     5461 tatgttaaca tttatcttcc tcctattcga atttcattct agcacaagaa aaaaactttt
     5521 gcacaattct gctacggaaa agacagaaaa agataaaaac gctttccaga caaaaaacta
     5581 gtaatttttt gacaagcttg ataaactaaa atcctatcta tttttatata agaaagaata
     5641 aatgttcgaa aataggaggt tttatgaaat cgaatcgtta tattattgcc tttgctgggg
     5701 tcattttaca cttaatgctg ggttcgactt atgcttggag tgtttatcgt aaccctatta
     5761 ttgaaaaaac gggatgggat caggcttctg ttgccttcgc ctttagtcta gcaatctttt
     5821 gtttgggctt atcggctgca tttatggggc gtttggtaga aaaatttggt ccgaaagtca
     5881 tggggagtct atctgctttt ctatacgcag gtggaaatat cttaacagga tttgcaatag
     5941 accgtcagga actgtggttg ttgtatctag cttatggcat tttaggtggg cttggtttgg
     6001 gagcaggcta tattacccct gtgtcaacga ttataaaatg gtttcctgat aaacgtggtc
     6061 tcgcaacagg tttagcgatt atggggtttg gttttgcttc tttattgact agtcccatag
     6121 cgcaacacct catcgcaggg gtagggattg tagaaacttt ttatatttta ggagcaagtt
     6181 actttattat catgctccta gcttcacaat tcattaagcg tccaaatgag caagagcttg
     6241 caattttatc ttcttcaggg aaagaaaaaa cagcctcttt gacgcaagga atggctgcaa
     6301 atcaggctct aaaaagcaat cggttttata tgctttggat tattttcttt atcaacatag
     6361 cttgtggttt aggcttaatt tcagcggcat cgccaatggc acaggagatg gctggcttgt
     6421 ctacaagtca tgcagcagta atggtgggtg ttttggggat tttcaatgga tttggtcgct
     6481 tgctctgggc gagtttgtct gactatatcg gtcgccctct aacctttagt atattactgc
     6541 ttgtcaatct tttcttttct ctctcacttt ggctctttac agattccgtt ttatttgtag
     6601 ttgctatgtc tattttgatg acttgctatg gagctggttt ttctttgatt ccagcttatc
     6661 tcagtgatat ttttggaacc aaggaattgg ccgctctgca tggttatatt ttaacagctt
     6721 gggcaatggc tggtttagcg ggacctattt tattagcaga gacttataaa atggctcatt
     6781 cgtacacaca aaccttgttc gtttttctca ttttatacag tatcgccttg gctttgtctt
     6841 attatctagg tcgttcaatc aaaaaagaaa gtcaaaaacc gcttacatga ttttctgtag
     6901 aaatggggct atcttttgct ctggtcttgt gttatactag ataggttgca aagaaaacag
     6961 tacttttctt ttgtggaaaa gaagcaacac gattttatat ctagttatat gaaaatacgt
     7021 cataaaaaga aaagtataaa ctaagcccta tagggtggct tcgcaccacc tttagaaaga
     7081 agaataacgt gaaatttaat gaattaaact tgtctgctga tttgctagca gaaattgaaa
     7141 aagctggttt tgtagaagct agtcctatcc aagaacaaac tattcctttg gcccttgaag
     7201 gcaaggatgt tatcggtcaa gctcagactg gtacaggaaa aactgcagcc tttggcttgc
     7261 ctacccttga aaaaatccgt atagaagaag cgactattca agccttggtc atcgctccaa
     7321 ctcgtgaact agctgtccaa agtcaagaag aactcttccg ctttggtcgt agtaagggag
     7381 tcaaagtccg ttcagtatat ggcggatcaa gcattgaaaa acaaattaag gctcttaaat
     7441 ctggtgccca tatcgtggtg ggaactccag gtcgcctctt ggacttgatt aaacgcaagg
     7501 ccttgaaatt acaagacatt gaaaccctta tccttgacga agcggatgaa atgcttaaca
     7561 tgggcttcct tgaagacatc gaagccatta tttcccgtgt acctgagaac cgtcaaactt
     7621 tgcttttctc agcaactatg ccagatgcca tcaaacgtat cggtgttcag tttatgaaag
     7681 cccctgaaca tgtcaagatt gcggctaagg aattgacaac agaattggtt gaccagtact
     7741 atatccgtgt taaggaacaa gaaaaatttg acaccatgac tcgtctcatg gatgtggcac
     7801 aaccagaact cgctattgta tttggtcgta ccaaacgccg tgtggatgaa ttgactcgtg
     7861 gtttgaaaat tcgtggcttc cgtgcagaag gaattcatgg cgacctagac caaaacaaac
     7921 gtcttcgtgt ccttcgtgac tttaaaaatg gcaatcttga tgttttggtt gcgacagacg
     7981 ttgcagcgcg tggtttggat atttcaggtg tgacccatgt ctacaactac gatattccac
     8041 aagatcctga gagttatgtt caccgtatcg gtcgtacagg tcgtgctggt aagtcaggtc
     8101 aatctattac ttttgttgct ccaaacgaaa tgggttacct tcaaatcatt gaaaacttga
     8161 ctaagaaacg catgaaaggt ctcaaacctg caagtgtaga agaatccttc caatcaaaaa
     8221 aacaggtagc tctcaagaaa atcgaacgtg attttgcaga tgaaaccatt cgtgccaact
     8281 ttgagaaatt tggtaaggat gctcgcaaat tggctgctga gtttactcca gaagaattgg
     8341 caatgtatat cttgagtctg acagtccaag acccagatag ccttccagaa gtggagattg
     8401 cacgtgaaaa accactaccg tttaaaccat caggtaatgg tttcggtggt aaagctaagg
     8461 gaggtcgtgg aggccgtcgt ggggacgacc gtcgagagcg tgatcgtcgt ggcaatggtc
     8521 gccgtgatga gttcaaaaaa ggaagtcgtg gcaacgatcg ttttgataag gaaaaacgtt
     8581 accgtaagga taataaaaaa ccacgcaata ctttaagcga aaagcaaaca ggctttgtta
     8641 ttcgtaacaa aggtgataaa taagaaaaag cggttcaaaa tgaatcgctt ttttatataa
     8701 ggagaccgaa tgtgaaaaag acaaataaaa tt
//
LOCUS       DAVMGZ010000086         8567 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000086 DAVMGZ010000000
VERSION     DAVMGZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.087"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(32..448)
                     /locus_tag="VYQ93_001509"
     CDS             complement(32..448)
                     /locus_tag="VYQ93_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3336177.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(460..1155)
                     /locus_tag="VYQ93_001510"
     CDS             complement(460..1155)
                     /locus_tag="VYQ93_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3336178.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(1349..3850)
                     /locus_tag="VYQ93_001511"
     CDS             complement(1349..3850)
                     /locus_tag="VYQ93_001511"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV3336179.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLIRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(4305..5393)
                     /locus_tag="VYQ93_001512"
     CDS             complement(4305..5393)
                     /locus_tag="VYQ93_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV3336180.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(5411..6079)
                     /locus_tag="VYQ93_001513"
     CDS             complement(5411..6079)
                     /locus_tag="VYQ93_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV3336181.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHIAATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(6098..8536)
                     /locus_tag="VYQ93_001514"
     CDS             complement(6098..8536)
                     /locus_tag="VYQ93_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV3336182.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
BASE COUNT         2532 a         1891 c         1471 g         2673 t
ORIGIN      
        1 ttaaattttg cttaagaaaa aacaagtttt gttatttttt acgattcatc caagtcatcc
       61 ctatacaatt ataggtggat aagatttcaa agcccataga acgatagaag cccaaggttc
      121 tttctgtctc ttctgtcacc agctggactt gataggcatc tttgtaatcc tctaaagcct
      181 ctttcatcaa ggcactacca attccttgac gctgatagat tggtaaaacg attaaatcct
      241 gaaccaatac tgatgagaat ccatctccaa ccaaacgaat caagcccacc acagcatcac
      301 catcaagtgc cagataaatt actaatgaat gagataaggc ctgctccagc atctctggtt
      361 gatgggtata atttgtccaa ccgacagcct gatagagatg taaaacatcc tctagcttga
      421 caatttcttg ctttttaata gtaatcatct caacacctct taaagttctc tcaagctctt
      481 gtactgctgt ccatttttat caaaattttc aggacgcaac catgtttcca aacgatcttt
      541 gacttgaggc cagtccttat caatcataga caaccaatcc gtatctctcg tacgtccctt
      601 ataaaccact gcctgacgga aggttccttc ataaataaag ctcaaacgct ccgcagctcg
      661 tcttgatggc agatttaagg catcgcattt ccactcgtag cgacgatagt ttagctcctc
      721 aaaaacatag cgagctagga gatactgagc ttcagttcct atccgtgtac cccttagctc
      781 tggagaaaaa gtgactgctc cgacttctat tactcggtta ttctggtcaa tgcgcatgag
      841 agaaaaagat cccaaagcct taccagtcgc cttgtctatg attgcatagt aaaaacggtc
      901 cttacgagcc aacatctgat gtaaaaggct aaccagctcc cccatatctg ctactggctc
      961 ctgaaaaagg taggtccaca tctcccgagg agtatccggg ccataaacag ctagcaaatc
     1021 ctccgcatgc ttttccactg aaagagcctc tatcctagca taacgccctt ctaagaaatc
     1081 aatagaaggc agttcacctg gtgtataacc ttccattgac tccccaatca tttgaccata
     1141 ttcatttact ggcatagctt tctccttttt tcacttttca aactttatag ttgcccatag
     1201 tataccatac tttcataaga agattcaaaa aatcctccag attctactct gaaggattcc
     1261 taccttattt cacaacgata ttaacgagtt tagttggctc aaacagtttg ggaaactgtt
     1321 tgaggttgga aatcagcaaa taaattggtt attttacaac aatattaacc aatttatttg
     1381 gtacactaat cactttcacg atttccttac cgtcaatttc tgctttgact ttttcatccg
     1441 ctagagcaat ttcttgcaat tcttcacgtg ataggtcttt agcgaccatg agtttggcac
     1501 ggactttacc tttgatttgg acaacgattt cgatttcgtc ttcaaccagt ttactttcgt
     1561 cccaagttgg ccaagctaca taagagattg actcacctgt ttctgcgact gtttgccaga
     1621 gttcttctgc caagtgaggt gcaaatggtg caatcaattg aataaagcct ttggcatagt
     1681 caacataaag cttatcttcc ttgttagcag cattgacaaa gaccataagt tgggcaatag
     1741 ctgtgttgaa tttgagagac tcaatttgct cagtaacagc tttgactgtt tcgttgtaag
     1801 ccttgtcaag agcaccattg ttttccgcaa ggatttcttt acttgtaatc aaacggtaaa
     1861 ctcggtcaag gaacttacgg cttccttcca aaccttcttc tgaccaagca atcgaagcat
     1921 cgagtggtcc cataaacatt tcataaacac gaagggtatc ggcaccgtat tgttccacca
     1981 catcatctgg gttaacaacg ttcttgagcg atttagacat cttggctggc gcttgctcca
     2041 actcttcccc tgtttctata tgaaagaagg aaccatcacg tttttcaacc ttgtcggttg
     2101 ccacaagagc accacggtgg tcacggtagc ttgttcccaa aatcatccct tggttaaaga
     2161 gtttttggaa tggttcctta gtcggaacaa caccgaggtc atagaggaat ttatgccaga
     2221 aacgagcata aagcaagtga agtacagcat gttccgcacc acccacgtag atatctactg
     2281 gcaaccattg tttgaggagg tcctcatcag ccaatttctc agtattgtgc gggtcaatat
     2341 agcggaggta gtaccagctt gaaccagccc attgtggcat ggtgttagtt tcacgacgac
     2401 ctttgacacc atcttcacga gtcacttcaa gccaatctgt caagttagct agtggacttt
     2461 caccagtacc tgaaggacgg atatccttgg ttacaggcaa gacaagcggc aattcagttt
     2521 caggaacagc tgttgaagtt ccatcttccc aatgaatgat tggaattggc tcaccccagt
     2581 aacgttgacg gctaaagagc cagtcgcgga ggcggtaggt aaccttctcc tgaccacagc
     2641 ctttttcttc caagcaagcc acaatcttgg caatagcgtc ttctttgttc aatccatcta
     2701 ggaagtctga attgacatgc aggccatcct ctgtgtaggc agcttcttcg acatttccac
     2761 cttcaagtac ttcgacgatt ggaaggtcaa attgtttggc aaattcccag tcacgttggt
     2821 cgtgggcagg cacagccata accgcacctg ttccataact agcaagaaca tagtccgcaa
     2881 tccagattgg catttcctta ccattgacag ggttgatggc ataggcacct gtccaaacac
     2941 cagttttttc cttagcaagg tctgtacgaa tcaagtcaga cttaaggctg gcttggtgtt
     3001 tatagtctgc tacagcttct gcttgctctg aacttgtgat agcgtctact aattcatgtt
     3061 caggagccaa gacagtgaaa gtcgcaccga aaagtgtgtc cggacgagta gtaaagactg
     3121 taaattcctt gtctgttcct tttactttga aagttacatt ggcaccagtt gatttaccaa
     3181 tccagttgcg ttgcatatcc ttgatagact ctgaccaatc tagttcatct aagtcattga
     3241 gcaagcgctc tgcgtaagcc gtgattttga gcatccattg gcgcattggt ttgcggacaa
     3301 ctggatagcc tccacgctca gaagttccgt caggaagcac ttcttcattg gcaatggcag
     3361 ttcccaattc ctcaacccag tttactggca cttcagcttc ataggccaag cctttttcgt
     3421 aaagcttggt gaaaatccat tgagtccact tgtagtagtt tggatctgtt gtgttgactt
     3481 cacgatccca gtcataagaa aatccaagcg cattgatttg acgtttgaag ttggcaatgt
     3541 tttccgctgt aaattctgct gggtcattac cagtatccat agcgtattgc tctgcaggca
     3601 aaccaaaagc atcccaaccc attgggtgaa gaacattgta gccttgcgca cgtttgtaac
     3661 gactgaggat atcggttgca gtataacctt ctgggtgtcc tacgtgcaga ccagctccag
     3721 acggataagg gaacatatca agcgcataaa acttaggttt tgatgcatct gttcctgtct
     3781 taaatgtatg atgttctgcc cagtagccct gccacttagg ctcaatttct ttatgattat
     3841 aaaaactcat ggtctctctc caattttgtg atgttactat tataccattt ttgagggaat
     3901 ttgaaatgtg tttaaaggaa aaactgtata ctgtatctca ttaattttga tgactatgaa
     3961 ttgctctatc tcccactttt tatcccattg ttatgcagta aatcaaggag taagtcaaga
     4021 agcttatctc gtatagcaca aaatacttat tttagaccga agataaaact ggaatgctaa
     4081 ttaatgtatt cacaataaat agccgacaac ctatgtcgct ttttgattta tgaagaacgt
     4141 atagtgcact gaatctgaaa tagtacgaaa caattgctaa aacattgtta gaaattaatt
     4201 ttactttccc aatcgatttg ttctcatctt atttcaatct gctatagaat tcaatacgag
     4261 gatcaataaa agactaccat ttggaaaaca gtagtcctta ttgtttattt tgaatttaca
     4321 taggcatcta cagcgataat agcttgagca acatctgaag gcgaaatctt aaacggcatc
     4381 tgatgaattg tctcaccctc catagttgct tgtttaccaa cttttattaa atcatcatat
     4441 ccaacttgat ccaaatgcat ttcttttaga gttgttggca taccaatttt tttgtaaaac
     4501 tcaatatact tatcaagttc ttctttaggt ctattttcca ataatagttg tactaaagtt
     4561 ccataagcta ctttttcacc atgtgttaaa tgatgaatgt cacctgtcaa tgcagtaaaa
     4621 ccattatgaa ttgcatgcgc cgcagctaat cctccacttt caaaacctag accactcaat
     4681 aaagtattag cttcaacaat attttctaat gctggtgtca ccactttagc ttcacaagct
     4741 gccatagcct gtaaaccatc tgcaaacagc gtttcttcac atttcttcgc aattgcaact
     4801 ccagccaatg tttgctgttg tcccaacata gtttttccat ttgcctgcat aaccgcacgc
     4861 gcctcaaccc aagttgctaa accatctgca ataccagacg ctaataaacg cttaggggct
     4921 tgtgaaataa cttttgtatc aaccaaaact aaatctggat ttttagaata aaatagataa
     4981 tgatcaaatg caccttcatc tgtataaata acagataaag cagatacagg tgcgtcggtc
     5041 gatgcaattg ttggagcaat aataacaggc ttttcaatca aatctgcaat agcttttgcg
     5101 ctatcaatcg tctttccccc accaagaccg ataatactat cacaattttc tttctcagcc
     5161 aaggcaacaa ctcgattgat ttcattgtca gaagcttcac cattaaatag cgccagaaca
     5221 atatggaaac catacctatg tagataatct tcaaatcgtt ttccaacaat atcataaacc
     5281 aactgatcgc ataatagaat agggcaattt cccaaatcca aaattgattt ggcattttca
     5341 aacaaggcat tttccccctg aatatatcta gaaggactag caaaaattct catatgtatt
     5401 tcctctctat ctaaatggaa cgactatttt gaataacaaa ccagtcgtca gaaaagtcat
     5461 caaccgcctt ttggatagat ggcatggcga aagctgattc aaaaacatct gctcctgctg
     5521 taacagcatg cgcacctgca gctaaagcat tatttacttg tgctacattt ttaaaggatg
     5581 cagctaaaat cttactagga gagttctgtc tatcaatagc aagagctaat tgacgaatga
     5641 cagaatttga atcaatgttc agattttcca ttctattata atatggagct aggtaatccg
     5701 ctcctgcttc gatagctaat aatccctgaa taactgtata aatagctgtt gcagcgatat
     5761 ggtagccctc tttttttagc acctttattg cacgtaatcc agctggagta acaggtactt
     5821 tgataaatat atcatctcct gcttgtcttc gaatttcatg agcatccttt aagatgcctt
     5881 caaaatcttg agaaatcacc tgaacatgaa tagagggtgt agagccaatc aattctctta
     5941 catctttgat tcgttcaaaa aaattaatag aaccctctct ttttgcaata gtgggatttg
     6001 aagttacccc agctagcggc aaaatttcag accacttttt aatctcatct aaatttaatg
     6061 tgtcaagcat aaattccata aaaagaacct ctttatttta caaagtatgc tcagtacgtc
     6121 ctataatgtc atcttgggtt gccttagaaa gaacattgaa gaatgcagag tatcctgcaa
     6181 cacgaacaat taagtctctg tgtttttcag gatgtttctg agcgtcaatc aacgtctctc
     6241 tggaaacaac attgtattga atatggtacc catgtaaacg attaaagaat gttcgtaaca
     6301 aagcaattag ttttaattta tcttcttctt tggctaacgt ttgaggattt actttctgat
     6361 ttaagagaac cccacctacg atttcatctg ttggtaattt tgaaacagat tttaaaacag
     6421 atgtagggcc gtgttgatcc atattatgtg atggtgaaca accctctgct aacggtgtac
     6481 ccgcgttgcg tccatctgga gttgctaatg ttccacgtcc ctgccctacg ttggctgata
     6541 tagaagatgt tcctgaataa cgaattcctc caataggccc tcttccataa cgtgtattag
     6601 gatatttagc aatttcatca acataaatgt cataagcagc agtaaccaat ttgtcagcat
     6661 aatcatcatc attaccatac ttaggtgcat catgaatcaa catttcttga atgaccttac
     6721 cttcttctcc ggcataatct gtttccagtg catgccaaag ctgacttggg cttatacgtt
     6781 cttcctcaaa caccaatttt ttaattgcag ctaatgaatc cgacaaattt gcaattccaa
     6841 cttgcaatcc tgatatataa tcatatactg ctccaccttc tttaaggtgt tttccacgac
     6901 caatacaatc atcagtcaat gctgaacata gaatatcagg aacttctcgt tccaatgata
     6961 aatcaataga attttcaaca ataacactca ttcgtgtcaa atatcttagt gttttatccc
     7021 aagcattttc taattcagaa aagttcttca tatccttaaa atgaccaaag cttggtgcaa
     7081 accgtttacc cgaagccgga tcaattccat cattcatcgt gataagtaga accttaggga
     7141 agttcatata actcatacct gtgcaacgat agccccattt ccctggaact gccgtttcaa
     7201 cacatccaat ggcactgtaa tcataagcat catcttccaa tactcctttt gcaataaaag
     7261 aaggaataat aatctcatca ttattaaatg caggcatacc aaaaccaagt ttcatcactt
     7321 caatacactc attcatgaaa cgagcatcta aacctgcatg gtaacgtaca gttagattag
     7381 gttgcggtag atgggtttgt gcaactgatt ttaataccaa ataagataat gggttaacag
     7441 catccttctt atctcgagtc tgtccaccaa ttgtaacatt ttgatataaa ggacttcctg
     7501 ctgaagaaaa tgtatgtgat tgactgcgaa ccttattaat tgtaattgtc ttaatccaaa
     7561 gatttgtcag acgttcaaca atgctatctt ctgtttcttt accactttct aaatcagcct
     7621 tcatatatgg atacatatat tgatcaaaac ggccatatga aagagagtgg ccattagatt
     7681 caatttgtaa aatacattga ataaaccaaa ctgattgaat agcttctgca aaagtagttg
     7741 ccggttcata tgggactcta gagcaaatat ctgcaatctc aagtaattct ttcttacgtt
     7801 taggatttgc attttcggct aaacttttag caagagcaac aaagcgcttt gcatatactt
     7861 taatagcatc gattacgata aatatagagt cgtaaaaatg atatttatca atacttgctg
     7921 gatctgttaa atctagagct accttcgctt tacgagcccg ctcttcaaaa cctcttaaac
     7981 caaattgcaa aagtttctga tagttaactg ctaagtgagc atctccagaa ttcatcttac
     8041 cttccatacc gaagaatcct gtttccatat aaacagacac ttcttcaggt aataaggcac
     8101 cagctctagc acgtaaatta ttattttccc aaaacggagc aatacttcta agttgttctt
     8161 ttgtttcttc tgtaatatag aaaacatctc catcacgctt ttcaaaaaga tccaactcat
     8221 tgagaacaaa ttctagcgta tattccggaa aaataggagc atctttattg gaagaagctt
     8281 gatttcccgc aatcatagat tcttcttcaa tatagataga catattttcc aaaatttctt
     8341 tcagcatata tgcacgtttt aggacattag gttgttcctt gtatcgttca taggcgcgtg
     8401 ttgctagaac tgctctctca gcatcaatat aaggtttttt atttaaaaca tcttctcgat
     8461 atttgttcat cctttcagta aggctgccaa aatattctgt cttgattgtt gttcttgcta
     8521 cttctgtatt taccatcgta agcacctttc tttcgaaaat attttta
//
LOCUS       DAVMGZ010000087         8390 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000087 DAVMGZ010000000
VERSION     DAVMGZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.088"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            235..1296
                     /gene="dinB"
                     /locus_tag="VYQ93_001515"
     CDS             235..1296
                     /gene="dinB"
                     /locus_tag="VYQ93_001515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV3336183.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLRIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWKELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVQSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            complement(1443..2288)
                     /locus_tag="VYQ93_001516"
     CDS             complement(1443..2288)
                     /locus_tag="VYQ93_001516"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV3336184.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            2544..3412
                     /locus_tag="VYQ93_001517"
                     /pseudo
     CDS             2544..3412
                     /locus_tag="VYQ93_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3713..5602)
                     /locus_tag="VYQ93_001518"
     CDS             complement(3713..5602)
                     /locus_tag="VYQ93_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV3336185.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRGA
                     KAADEKRIQARGIQLKSSFEAVLKEMQEGVRKRVVFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            5740..7305
                     /locus_tag="VYQ93_001519"
     CDS             5740..7305
                     /locus_tag="VYQ93_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV3336186.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            7305..8045
                     /locus_tag="VYQ93_001520"
     CDS             7305..8045
                     /locus_tag="VYQ93_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3336187.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(8154..8369)
                     /locus_tag="VYQ93_001521"
     CDS             complement(8154..8369)
                     /locus_tag="VYQ93_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336188.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         2650 a         1842 c         1580 g         2318 t
ORIGIN      
        1 aataaagtcc ttttttcttt ttttcagaaa aaagggtata ataaaagaaa ataagcagtt
       61 acactcaatg gaaatcgaaa agcaaactag gaaactagcc gcaggctgct caaagcactg
      121 ctttgaggtt gtaaatagaa ctgacgaagt cagctcaaaa tactgttttg aggttgcaga
      181 tggaagctga cgtggtttga agagattttc gaagagtata aaaaggtgct aggcatgttg
      241 atttttcctt tgttaaatga tttgtcaaga aaaatcatcc atattgacat ggatgccttt
      301 tttgctgcag tggaaatcag ggataatcct aaactcagag gaaaacctgt cattattgga
      361 agcgaccctc ggcaaacagg tggacgggga gtcgtttcta cctgtagtta tgaggcaaga
      421 gcttttggtg tccattctgc catgagttcc aaggaagctt atgaacgttg tccccaggct
      481 gtctttatct cagggaatta tgagaaatac aagtctgtgg gactccggat tcgagctatt
      541 tttaagcgct atacagattt gattgaaccc atgagcattg acgaagccta tttggatgtg
      601 acagaaaata aactcggtat caagtcagcg gtcaaaattg ctcgcctcat tcaaaaagat
      661 atctggaaag aactccatct aactgcttcc gcaggcgttt cttacaacaa attcttagct
      721 aaaatggcga gtgattatca aaaaccacat ggtttgacag tgattctacc tgaacaggct
      781 gaggattttc tcaaacaaat ggatatttcc aaatttcatg gagtaggaaa aaagacagta
      841 gaacgtcttc atcaaatggg cgtttttact ggtgctgatt tacttgaagt tcctgaggta
      901 accctaatag accgttttgg tagactaggc tatgatctgt atcgaaaggc tcgtggcatt
      961 cacaactctc cagtccaatc caatcgcatc cgtaaatcaa tcggcaagga gaaaacctac
     1021 gggaagattc tccgtgccga ggaagatatc aaaaaagagc tgactcttct atcagaaaaa
     1081 gtcgctctca atctacatca acaagaaaaa gctggaaaaa ttgtcatttt gaaaatccgc
     1141 tacgaggact tttcaactct taccaaacga aaaagtcttg ctcaaaaaac acaagatgct
     1201 agtcagataa gccaaatagc cctgcaactc tatgaagaat taagcaagaa agaaagaggt
     1261 gtccgcctat tggggattac catgactgga ttttaaagct caatgaaaat caaagagcaa
     1321 actaggaggc tagccgcagg ctgctcaaaa cactgttttg aggttgcaga taaagctgac
     1381 gcggtttgaa gagattttcg aagagtataa aaccttgaag agttgcccct tcaaggtttt
     1441 tcttatacaa ataaacggac aaaactgtaa agtagcaaaa cactaccaag cacgatacgg
     1501 tatttaccaa aaagggtgaa gtcgtgtttt ttcacatagc tggtcaagaa gcgaatagcc
     1561 accatgctga ccgcaaaagc tactcccatc gcgaccaaga gtaaaaacaa ttgcccaaag
     1621 ctcaagagtt ctccggcttt cacaaatttg aaaatcttta aggcactagc tccaaacata
     1681 acgggaatcc caagatagaa ggtaaattct gtcacaactg aacgactggt tccatttaac
     1741 aaaccaccga caatcgttgc acctgaacgg ctagtccctg gtaaaagagc aagaacttgg
     1801 aagagtccga tatagaaagc ggtcgtataa ggaagcttgt ccaactctgt tacacttggc
     1861 tcgatagcac gcgctttatt gcgcttttcc aaatatatga aggcaacccc gtagataatc
     1921 aacatgagag caactgaaac catgttatgg aagtgggtat caaaccaatc atcaaattta
     1981 aagacaccaa gtaaaggtaa agtagcaatc aagaccttca accatagtct ccaagtctta
     2041 cgaacttcct gtttgtcctt agtcggttta aaaggattga gcttgttaaa ataaatcacc
     2101 ataactgcta aaatagcacc aagctgaatc acgacattaa acatggacat aaaggcttca
     2161 ttttgatttt ggtattggat aaactcctct gctaaaatca agtgacctgt actggaaatc
     2221 ggcaaccatt ccgtaattcc ttcaacaatc ccgaagaaga tagattttaa aatttcaata
     2281 agatacatat atttctcctt atattatatt taacgcagtt agctactgcc aaagcccaag
     2341 tggtatactt ggaataagcc actgtggatt agttcatttc ctccaatcgc ttgacgattt
     2401 ccgaaagggt ctaacaacag ctttttgttt caattggtaa tgagtttgat aacgcataga
     2461 cgttttatct cgtctatggt aacaaggcaa aaagttcgtg gataaccaag tagacttgaa
     2521 atactatatc tggaggtact cccatgcttt atgtgggcat tgatgtcgct aaaaataaac
     2581 acgatgttac agccttgaat gttcgaggaa aactgttctt aaaccactca ctttttcaaa
     2641 taataaagct ggttttgaac tcttagatct gtcttttcga cagctcaacc aagactgtct
     2701 catcgctctt aaacttcttt ctgaccccaa tcgtgaacaa tttcaacacg ataatcggca
     2761 aatagaacta aaaatactgg ctagacatat tcatcgtctc aagaaaaaac agtctgattg
     2821 gaaagtacaa tacactcgtt gtcttgatat catctttcct gagttggata aaatcgttgg
     2881 aaagcattca gaatatacct accaactctt gacacgctac cctaatcctc agaaaaggct
     2941 tgaggcagga tttgataagc tgatagaaat taagcgattg accgcttcta aaattcagga
     3001 tatcctctca gtcgcacctc gttctatcgg aacaacatct cctgctcgtg aattcgaaat
     3061 catcgaaatc atcaaacatt acaagaggct cattgacaag gcggaaacat gtgtcaatga
     3121 cttgatggct gagttcaact cggtcatcac gacggttact gggattgggg gtcgtttagg
     3181 ggcggtcatt ttagccgaga ttcgaaatat tcatgccttt gataatcctg ctcaattaca
     3241 agctttcgct ggactggatt cttctattta tcagtcaggt cagattgatt tagctggaag
     3301 aatgatcaaa cggggttccc ctcatctgcg gtgggcactc atacaagctg ccaaagcatg
     3361 cgctcgcttt tcacctgctt ttaaggccta tcttaagact aagttagaat aaggaaaaca
     3421 ttacaatgta gccatcatcc accttgcaaa aaaacttatc cgaaccctgt tttatatcct
     3481 aaaaaagagc tgccatttga cgaacaaaaa gtgatgtaat ttctgaaatt aacacaggat
     3541 tacctttgaa aaaatggact ttttcaaggg ctttttgccg tgctctaaaa tcatcgccaa
     3601 atcatcaaat tgaaataaga aatagtttag ataaaactat tgacttttct tatagtttgt
     3661 ctttcttcta tcttccatta tatcatactt tttcagtcta tggtagctct ctttaaaagg
     3721 ctccgatact gccgccacct ccgcctccag agaagccacc accagaactt ccacttccag
     3781 aagatacaga gtaggtactt gctgtatttg cgacactagc ataatggctc atttgcgcgc
     3841 ttgaatgata aaacatactg tgccagccat aagctacata gagattgata tctggatttt
     3901 caacttgaat ctgatgaacc ttcatcaaat gactaacctt gtccgcatag ccaaataagg
     3961 ttgcatagac caagagacga ttccaaacaa caatactttc caattcagcc ttatccagtc
     4021 gtgcaatctc acgtaacata ttttcaaaac tggtccagag atagtagacc tcagctcccg
     4081 cttcatttag aacaccatca cgattatcta gtcgaagctt ccaataatag aaaacagaca
     4141 aaactaaccc tagaaaacca agtattggca aagggaggta aagatagcca tgaacgtcta
     4201 aactgtacaa gaacaaacca aatccgataa ataggggcaa gatagtcaag acacccatac
     4261 ccacttgcaa agccttttcc aaaccagtta aaggacgata gtaatctggg agtccccaga
     4321 agacaactcg ctttctcact ccttcttgca tctcttttaa tactgcttcg aaagaagatt
     4381 tgagctgaat ccctcttgct tgaatccgtt tctcatcagc agctttagct ccgcgataaa
     4441 ggctgtcaga taccttgtaa tccgcaaaca aattggaaag agtggcttct tttttgcctg
     4501 aaaaggctag atttagacag tctctctcaa atcttgacaa accatcttct ttcaccaatc
     4561 tcaagccaac tgcatctcct tctgaaataa tagaaacatt cccacggtcc atcacatcta
     4621 gcaaggtagc ttgaataagt tggtcaaaag tgaatttgcc agcccccttg actaagggac
     4681 tcacttcctc caaggaggtc gagtagacag cctctgataa aaccataggc tctaattcca
     4741 ttggtggttc atagagacga tgatttttgg catatttgac tgaaggagtg gtctttcttc
     4801 tataaataaa atagaagcag acactcaata acaaggagat agaaaggatc gaagggagga
     4861 cccaagtaac aagttgttta ctctgatctt tttctctaac aatcgagtct tctatcttat
     4921 taaactcttc taaacgattc cctttcaatc cctgatccct agcgctagca aaatcggtcc
     4981 gaggccagta ggcatgcaac tcaactccac gcttagccgg aagattgtct aaacggatag
     5041 tataatcaag gttactcttt tcaatcgttc cctctctaaa aagtttccct gtatggaaaa
     5101 agagtttttc agcccccttg tctcccctta catgaaattc aaacttttca atagactctg
     5161 aactatctgt cagaggttgc caatttaatt cagcgatatc atcataaagg aaaagtaaat
     5221 tttttaagtt ccagacgagg tcaacttcaa ctatgtcgcc ctcctgacct ggattataga
     5281 ctctcacagt ataaccatcc gcttcttctg ttacttcgct agtcacatct gctagttctg
     5341 caccgttttt cgcggcctga acctttggat gagggtcaat gtcaaacccg ctaggcatct
     5401 taccagcacg tccaagtccc acgatttgtc ccttaaagtc ctcctcaaac tggtaaacta
     5461 tcttctgtct aaactctgcc gtattatctg catgaatata caaatcccct tgataagagt
     5521 ttatcttgaa atcaatggca aaaacagaga gtggaagaag gcaaaacaag cctaacacca
     5581 gtaagaaaaa agtttttttc atcaactaag cccccttttt atctttctta tcattatagc
     5641 attttttcgt aagtaaaggc ttacttatgt tgaaaaatta cgcatttttc gataaaatag
     5701 gagacagtta ttttttaata gatcagaata ggagaaatca tgagaaaaat atacttatct
     5761 attttcacaa gtctcttgct gatgctagga cttgtcaatg ttgctcaagc cgatgaatat
     5821 ttacgcatcg ggatggaagc agcatatgct ccctttaact ggacccagga tgatgatagc
     5881 aacggagctg tcaaaatcga tgggaccaat cagtatgcca acggatacga tgttcaaatc
     5941 gccaagaaaa tcgctaagga cttaggtaaa gaacctttgg ttgttaaaac caagtgggaa
     6001 ggtctagtcc ctgcccttac ttctggtaag attgacatga ttatcgcagg tatgagtcca
     6061 actgcagaac gcaaacaaga aattgccttt tcgagcagtt actacactag cgaaccagtt
     6121 ttgcttgtca aaaaagattc tgcctacgca agtgctaaat ctttggatga ctttaatggt
     6181 gcgaaaatca cttctcaaca aggtgtctac ctctacgact tgattgcaca aatcccaggt
     6241 gctaaaaaag aaacagccat gggagacttc gctcaaatgc gccaagctct tgaggctggt
     6301 gtcattgatg cttatgtttc tgaacgtcca gaagcactga ctgctgaagc tgcgaactct
     6361 aagttcaaga tgattcaagt agaacctggt ttcaaaactg gggaagaaga tacagctatc
     6421 gctatcgggc ttcgtaaaaa tgacaatcgt attagccaaa tcaatgccag cattgaaacc
     6481 atttcaaaag atgaccaagt tgccttgatg gatcgtatga tcaaggaaca acctgccgaa
     6541 gctacaacaa ctgaagagac tagcagtagt ttctttagcc aagtcgctaa aattctttct
     6601 gaaaactggc aacaactctt gcgtggtgct ggtatcactc ttttaatctc tatcgtcgga
     6661 accatcatag gtctcattat tggacttgcc attggtgtct tccgtactgc tcctctctct
     6721 gaaaacaaag ccatttatgg ccttcaaaaa ctagtcggct gggttctcaa tgtctacatt
     6781 gaaattttcc gtggtacgcc aatgattgtt caatcgatgg ttatctacta tggaactgcc
     6841 caagctttcg ggatcaacct tgaccgtaca ctggctgcta tcttcatcgt ttcaatcaat
     6901 accggtgcct acatgactga aatcgtccgt ggtggtatcc tagcagttga caagggacaa
     6961 tttgaagctg cgactgctct tggtatgacc cataaccaga ccatgcgtaa gattgtccta
     7021 cctcaggtag tccgtaacat cctacctgca actggtaatg aatttgtcat caatatcaaa
     7081 gatacatctg tattgaacgt tatctctgtt gtcgaacttt atttctcagg aaataccgtg
     7141 gcaacacaaa cctatcaata cttccagaca tttacaatca tcgccgtgat ttactttgtc
     7201 ctcaccttca ccgtaacacg tatcctacga tttatcgagc gccgaatgga catggatacc
     7261 tatactacag gtgctaacca aatgcaaacg gaggatttga aataatgaca caagcaatcc
     7321 ttgaaattaa acacctcaaa aaatcctatg gacaaaacga agtgctaaaa gacatttcac
     7381 tcactgtcca caagggagag gtcatctcta tcatcggaag ctctggaagc ggaaaatcga
     7441 ccttcctacg ctccattaac ctacttgaaa caccaactga tggacaaatc ctttatcatg
     7501 gacaaaacgt cctcgaaaaa ggctatgacc tcacgcaata ccgtgaaaag ttggggatgg
     7561 ttttccaatc ctttaacctc tttgaaaatc tcaatgttct tgaaaacaca atcgtcgctc
     7621 agacaactgt cctaaaacgc gaacgcacag aagctgaaaa gattgccaaa gaaaacctgg
     7681 aaaaggtcgg catgggagaa cgctactggc aagccaaacc aaaacaactc tcaggtggtc
     7741 aaaaacaacg tgtggccatc gctcgtgccc tctccatgaa tccggacgct attctctttg
     7801 atgaaccaac atcagctctc gatccagaaa tggttggaga agtcctcaaa atcatgcagg
     7861 acctggctca ggaaggcttg accatgattg tcgtaaccca tgaaatggaa tttgcccgtg
     7921 atgtctctca ccgtgttatc tttatggata agggcgtgat cgctgaagaa ggtaaaccag
     7981 aagacctctt caccaatcct aaagaagacc gaacaaaaga gttccttcaa cgctatctca
     8041 aataaaaaga aaaggctgca tcaaccgtgc agtctttttg ctgtcctcaa aatgaaaagg
     8101 cagattctct atctctacta cttgaaggtc aagaatccgc cgttatccaa agattaatct
     8161 tcaaattttt cataattttt ttcatagaaa tcaattaagc ctagggctgt ttcaaatgaa
     8221 atatttttta cttttgcacg accctgcgcc agagcaatga tagacatttc acgcgcattc
     8281 gtttctttag agatacggta gcctgtgatt ttcttatcac gaacccagcc aacaactgat
     8341 tctacttttt caaagtttga cttagccatg tttttctcct attttcttct
//
LOCUS       DAVMGZ010000088         8158 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000088 DAVMGZ010000000
VERSION     DAVMGZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.089"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            104..2737
                     /gene="polA"
                     /locus_tag="VYQ93_001522"
     CDS             104..2737
                     /gene="polA"
                     /locus_tag="VYQ93_001522"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV3336189.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDQLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEIKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2822..3259
                     /locus_tag="VYQ93_001523"
     CDS             2822..3259
                     /locus_tag="VYQ93_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV3336190.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            3294..3374
                     /locus_tag="VYQ93_001524"
                     /pseudo
     CDS             3294..3374
                     /locus_tag="VYQ93_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(3532..4542)
                     /locus_tag="VYQ93_001525"
     CDS             complement(3532..4542)
                     /locus_tag="VYQ93_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV3336191.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            4691..5860
                     /locus_tag="VYQ93_001526"
     CDS             4691..5860
                     /locus_tag="VYQ93_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV3336192.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEVFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            5857..6627
                     /gene="recO"
                     /locus_tag="VYQ93_001527"
     CDS             5857..6627
                     /gene="recO"
                     /locus_tag="VYQ93_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV3336193.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            6624..7616
                     /gene="plsX"
                     /locus_tag="VYQ93_001528"
     CDS             6624..7616
                     /gene="plsX"
                     /locus_tag="VYQ93_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV3336194.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFVVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            7622..7855
                     /locus_tag="VYQ93_001529"
     CDS             7622..7855
                     /locus_tag="VYQ93_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV3336195.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            7901..8158
                     /locus_tag="VYQ93_001530"
                     /pseudo
     CDS             7901..8158
                     /locus_tag="VYQ93_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2323 a         1502 c         2085 g         2248 t
ORIGIN      
        1 ctaaattctc tggctctatc tatttattcg gaaaaagttt gaaaaatact tgctctagct
       61 cttcccaatg gtattttttg attctttcct ttataatggg tgtatggata agaaaaaatt
      121 attattgatt gatgggtctt ctgtagcttt tcgggcgttt tttgcgctgt atcagcagtt
      181 ggaccgtttt aagaatgcgg ctggtttgca taccaatgcg atttatggtt ttcagttgat
      241 gttgagtcat ttgttggagc gggttgagcc gagtcatatt ttggtggctt ttgatgcggg
      301 aaagacgacc ttccggacag agatgtatgc ggactataag ggtggtcggg ccaagactcc
      361 tgatgagttt cgtgagcaat ttcctttcat tcgtgagttg ctggatcata tggggattcg
      421 tcactatgag ctggctcagt atgaggcgga tgacatcatt gggacgctgg ataagctagc
      481 agagcaggat ggttttgata ttaccattgt cagtggggac aaggatttga ttcagctgac
      541 ggatgagcat acggtggttg aaatttccaa gaaaggtgtg gctgagtttg aggcctttac
      601 gccagattac ctcatggaag aaatgggcct cacaccagct cagtttatcg atctcaaggc
      661 gctcatgggt gataagtcgg ataatatccc tggggtgacc aaagtcggtg aaaagacggg
      721 tattaagctc ttgctggagc atggttcgct tgaggggatt tatgaaaata ttgatggaat
      781 gaagacttct aagatgaagg aaaatctcat caatgacaag gaacaggcct ttttgtctaa
      841 aacactagcg accattgata ccaaggcacc gattgcgatt ggtttagagg acttggtcta
      901 tagtggtcca gatgttgaaa atcttgggaa attttacgat gagatgggct tcaaacagct
      961 caagcaggct ttaaatgtgt cgtcagctga tgtggctgag ggtttggatt ttactattgt
     1021 tgaccaaatc agtcaagata tgctgagtga agagtctatc ttccactttg agctttttgg
     1081 tgagaattac catacagata atttggttgg atttgcctgg tcttgtgggg atcagctcta
     1141 tgccacagac aagcttgagc tgttgcaaga cccgattttc aaggatttct tagaaaaaac
     1201 atctctgaga gtttatgact ttaagaaggt taaagttctt ttgcaacgtt ttggtgtaga
     1261 tttgcaggcg cctgcttttg acatccgttt ggctaaatac ctcctttcga ctgtggagga
     1321 caatgaaatt gcgaccatcg ctagtcttta tggtcagact tacttggttg atgatgaaac
     1381 tttctacggt aagggtgtta aaaaggccat tcctgaacgt gagaaattct tggaacactt
     1441 agcttgtaaa cttgctgttt tggtagaaac agagcctatt ttacttgaaa aactcagcga
     1501 aaatgggcaa ttagagcttc tttatgatat ggagcaacct ctggcttttg tccttgccaa
     1561 gatggaaatt gctgggatta tggtcaagaa agagaccttg cttgagatgc aggctgaaaa
     1621 tgagcttgtc attgaaaaac tgactcaaga gatttacgag ctggccggtg aggagtttaa
     1681 tgtcaactcg cctaagcagt tgggcgtgct tctctttgag aaattgggac ttcctctaga
     1741 atacactaag aaaaccaaga caggttattc gacagcagtg gatgttttgg agcgtctcgc
     1801 tcctattgct ccgattgtta agaaaatcct ggattaccgt caaattgcta agattcaatc
     1861 tacttatgta attggcttgc aggactggat tttggctgat ggaaagattc atactcgcta
     1921 tgtgcaggat ttgacccaga ccgggcgttt gtctagtgtg gatccaaact tgcaaaatat
     1981 tcctgcccga ttggaacaag ggcgcttgat tcggaaggct tttgtgccag agtgggagga
     2041 tagtgtgcta ctcagctctg actattcaca gattgaattg cgcgttttgg cgcatatttc
     2101 taaggatgag cacttgatta aggccttcca agagggggca gatatccata cttcgacagc
     2161 catgcgggtc tttggcattg agcgtcctga taatgtgact gcaaatgacc gtcgcaatgc
     2221 caaggcagtt aactttggag tggtttatgg gatttcagac tttggcttgt ctaataattt
     2281 gggaattagt cgtaaggaag ccaaagccta cattgatacc tactttgaac gttttccagg
     2341 tattaaaaac tacatggatg aagtggtgcg ggaggcgcgt gataagggct atgtagagac
     2401 cctctttaag cgtcgccgtg agttgccaga tatcaattcg cgcaacttca atattcgtgg
     2461 ttttgcggag cgaactgcta tcaactcacc tatccagggt tcggcagcag atattctcaa
     2521 gattgccatg attcagctgg ataaagcctt ggttgcaggt ggttatcaga ctaagatgct
     2581 gttacaagtg cacgatgaaa tcgtccttga agtgcctaaa tctgaattgg tagagataaa
     2641 aaaattggtg aaacaaacca tggaagaagc cattcaactc agtgttcctc ttatcgcaga
     2701 tgagaatgaa ggggcaacct ggtacgaggc taaataaaaa gggggctagt cctccttttt
     2761 tgtagtagaa ttctgcaaac cttttcaaaa tgtgctatac tgatgaaaaa ggaggttttc
     2821 tatgagtcaa gaatttatca atccaagtga tggtgtgatt cgtcagtatc tcgcaacgag
     2881 taaaaccctt gctgtggtgg gtttgtctga ccgtgaagaa acaaccagca atcgagtaac
     2941 aaaggaaatg caggctcggg gctataaaat catcccagtc aatcccaagg cggcaggtgg
     3001 cgaaatcttg ggtgaaaagg cttatgctag cctcgctgaa attccttttc ctgtagatat
     3061 tgtcaatgtt tatcgtcgca gtgaatttct gcccgatgtg gcgcgtgatt ttctcaaagc
     3121 tgatgccaag attttttggg cacagctagg acttgaaagt ttagaagcga aagagatttt
     3181 gcgtgatggt ggatgtgatg atatcgtgat gaatcgctgt atcaagagag aacatactcg
     3241 cttgattgag gaagcataag aaaaaggtag ctgatgggct accttttgtg ttatactcaa
     3301 tgaaaatcaa agagcaaact aggaaactag ctgcgggttg ctcaaaacac tgttttgagg
     3361 ttgtagatac gactgacgaa gtcagctcaa aacaccgttt tgaggttgta gatagaactg
     3421 acgaagtcag ctcaaaacac cgttttgagg ttgtagatag aactgacgaa gtcagtaaca
     3481 tatatacggc aaggcgacgt tgacgtggtt tgaagagatt ttcgaagagt gttagaaaat
     3541 gccgataagg gtctgcatac caagagtggt gaggatgatg gcaatccagc agatggctcc
     3601 aagaagaatg gattttccac tggatttgac catagcgacc agattggttt tgagaccgat
     3661 agcactcatg gccatgacaa taaggaattt agagagttgt ttgagagggg taaagaaact
     3721 actggacaca cctagagagg tgagtagtgt agtgaggaga gaggcaagga taaagtaaag
     3781 gataaaaagt gggaagactt ttttcagttg taggctttgc ttgttttctt gttggcgact
     3841 ttgccagtag gatagaaaga gcgtgatagg gataatggcc aaagtacgtg tgagtttaac
     3901 aatggttgca gactcgaggg tattgctttg gtaaagactg tcccaagcgc tggcggcagc
     3961 cgttacagag gaagtgtcgt tgaccgcagt ccctgcaaag agggcgaagc cttcattgga
     4021 tagatgaagc caggtgccga gggttggaaa gatgagcgca gccaagacat tgaagaaaaa
     4081 gataacggaa atggcttggg ctacttcctt ttccttagca tggataacag gcgctgtcgc
     4141 tgcaatggca gaacccccac agatagaaga acctactcca accaaggtag ccagttttgt
     4201 atccagtgca aagaaacgct ggaagaggta ggcaataatc agagctattg agatagtgga
     4261 caggatgaca gggagtgaag attggccaac tgcgaagacc tgcgagatat tgagaccaaa
     4321 accaagcaag acaacggcat attggagcaa cttcttggaa ctaaaggtca aaccagcatc
     4381 cagttgttta taggacgaga gaaagggatg taggagcatg cctatgaaaa tggcaaaaac
     4441 gggcgcgcca atcacaggga agaatcctcc taagtaccaa gataggatgg aaatgagaag
     4501 gcaggccaag atacctgctc cattttttga tagaaatgac ataaaaacct ccaaatagaa
     4561 tctgttacca ttatagacct ataaacagga aaagtaaaat agaaagtgga aagctattct
     4621 ctaatgtatt tttgcggtcg gggggctttt gtagtataat agagatacgt tttgaaagta
     4681 ggaggtatct atggacttaa ctaagcgctt taataaacag ttagataaaa ttcaagtttc
     4741 gttgattcgt cagtttgacc aggttatttc ggagattcct ggggtcttgc gtttgacctt
     4801 gggggaacct gattttacaa cgccagacca tgtcaaggag gcggccaagc gagcgattga
     4861 tcagaaccaa tcctactata cagggatgag tggtctgctg actctacgtc aggcagccag
     4921 tgactttgtt aaggaaaagt accaactgga ctatgctcct gaaaatgaaa tcttggttac
     4981 aattggggcg acagaggctt tatctgcgac tttgacggct attttggaag agggagacaa
     5041 ggtacttttg ccagctcctg cttatccggg ttatgaaccg attgttaact tagttggggc
     5101 agaaattgtt gagattgata cgactgaaaa tggttttgtc ttgactcctg agatgttgga
     5161 gaaggccatt ttggagcagg gtgataagct caaggcggtt attctcaact atccagccaa
     5221 tccgacagga attacctaca gtcgagagca gttagaggcc ttggcagctg ttttacgcaa
     5281 gtacgaagtt tttgttgtct gtgatgaggt ttactcagaa ttgacctaca caggcgaagc
     5341 ccatgtgtct ctaggaacga tgttgagaga ccaggctatt attatcaatg gtttgtctaa
     5401 atcgcatgcc atgacaggtt ggcgtttggg gctgattttc gctcctgcga ccttcacagc
     5461 ccagttaatc aagagtcacc agtacttggt cactgccgca aataccatgg cgcaacatgc
     5521 tgcggtagaa gccttgacgg ctggtaaaaa tgatgcggag cccatgaaga aggaatatat
     5581 ccagcgtcgg gactatatca tcgaaaaaat gactgctctt ggttttgaga ttatcaaacc
     5641 agacggtgcc ttctatattt ttgctaaaat tccagcgggc tacaatcaag actcctttgc
     5701 ttttctgaag gattttgctc agaagaaggc cgttgccttt atccctggtg cagcctttgg
     5761 acgttacggg gaaggctacg tccgcctatc ttatgcagcc agcatggaga ctatcaaaga
     5821 agccatgaaa cgacttgagg agtacatgag agaagcatga ttcagtctat cacgagtcaa
     5881 ggcttggtgc tttacaatcg caattttcgt gaggatgaca agctcgtcaa aatttttaca
     5941 gagcaggttg gcaaacgcat gttttttgtc aaacacgctg gtcagtctaa gctggcgcct
     6001 gttattcagc ccttggtgct ggcacgattt ctcttgcgaa tcaatgatga cggactcagt
     6061 tacatcgaag actatcatga ggtcatgact tttcccaaga ttaatagtga cctctttgtc
     6121 atggcctatg cgacctatgt ggcagctctt gcagatgcta gtttgcagga caatcagcag
     6181 gatgctccct tgtttgcttt tttgcaaaag actttggagt tgatggaagc aggcttggat
     6241 tatcaggttt tgaccaatat ttttgaaatt caaattttga ctcgatttgg aatcagcctc
     6301 aattttaatg agtgtgtctt ctgccatcgg gttggtcagg cttttgactt ttctttcaaa
     6361 tatggagcct gcctctgtcc agagcattat catgaggata agagacgttg tcatctcaat
     6421 cccaatatcc cctatctgct caatcaattt caagctattg attttgagac tttggagacc
     6481 atttcgctca agcctggaat caagcaagag ctacgccaat ttatggatca attatatgaa
     6541 gagtacgttg ggattcacct aaaatcaaag aaatttattg attccctagc agactgggga
     6601 caattactaa aagaggaaaa gaaatgaaaa aaatcgcagt agatgccatg gggggcgatt
     6661 acgcacctca ggccattgtt gagggtgtca atcaagccct atctgacttt tcagatatcg
     6721 aggttcaact ttacggagat gaagctaaaa tcaagcaata tctgacagcg acagagcgcg
     6781 tcagcattat ccatacggat gagaagattg attcggatga tgaacctacg agagctatta
     6841 ggaataagaa aaatgccagt atggtattgg cagccaaggc tgtcaaagat ggtgaagcag
     6901 acgctgtcct ttcggctggg aatacaggtg ccttgttggc agcaggattc ttcgtcgtgg
     6961 gtcgtatcaa gaatatcgac cgtcctggac tcatgtctac cttgcctacc gttgatggaa
     7021 aaggttttga catgctagac cttggtgcca atgcagaaaa tacagcccag cacctccatc
     7081 aatatgcggt tctaggttcc ttctatgcta aaaatgtccg tggcattgcg caaccacgcg
     7141 ttggtttgct caacaacgga acagagagta gcaagggcga cccgcttcgt aaggaaactt
     7201 atgaattact ggcggctgat gaaagtttga actttatcgg aaacgtggaa gcgcgtgatt
     7261 tgatgaatgg cgttgcagat gttgttgtgg cagatggttt cacgggaaac gctgtgctca
     7321 aatccatcga agggacagct atgggaatca tgggcttgct caagacagct attacaggtg
     7381 gtggtcttcg agcgaaacta ggtgccctcc ttctcaagga cagcctcaga ggtttgaaaa
     7441 aacagctcaa ttattcagat gttggtggag cggtcttgtt tggtgttaag gcacctgttg
     7501 tcaagactca tggctcaagc gatgccaagg ctgtttatag tacgattcgt cagatccgta
     7561 ccatgctaga aacagacgtg gttgcccaga ctgcgcgtga attttcagga gaataaaaga
     7621 gatgacagaa aaagaaattt ttgaccgtat tgtgaccatt atccaagagc gacagggaga
     7681 ggactttgtc gtgacagaat ccttgagtct gaaagacgat ttggatgcgg attctgttga
     7741 cttgatggag tttatcttga ctctggaaga tgaatttagt atcgaaatca gcgatgaaga
     7801 aattgaccaa ctccaaaacg taggagatgt ggttaaaatc attcaaggaa aatagcaatc
     7861 ggagttccaa gtcaacggaa gtagatggtt tttagaaatg agaaatatcg gacaagctgg
     7921 aaaaatcttg gctgacagtg gttatcaagg gctcatgaag atatatcctc aagcacaaac
     7981 tccacgtaaa tcaagcaaac tcaagccgct aacagttgaa gataaagcct gtaatcatgc
     8041 gctatctaag gagagaagca aggttgagaa tatctttgcc aaagtaaaaa cgtttaaaat
     8101 gttttcaaca acctatcgaa atcatcgtaa acgcttcgga ttacgaatga atttgatt
//
LOCUS       DAVMGZ010000089         8118 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000089 DAVMGZ010000000
VERSION     DAVMGZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.090"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..109
                     /locus_tag="VYQ93_001531"
     CDS             <1..109
                     /locus_tag="VYQ93_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEV3336196.1"
                     /translation="VETFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            300..2591
                     /gene="pcrA"
                     /locus_tag="VYQ93_001532"
     CDS             300..2591
                     /gene="pcrA"
                     /locus_tag="VYQ93_001532"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV3336197.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGPGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2634..3314
                     /gene="radC"
                     /locus_tag="VYQ93_001533"
     CDS             2634..3314
                     /gene="radC"
                     /locus_tag="VYQ93_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV3336198.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3311..4000)
                     /locus_tag="VYQ93_001534"
     CDS             complement(3311..4000)
                     /locus_tag="VYQ93_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV3336199.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4018..4659)
                     /locus_tag="VYQ93_001535"
     CDS             complement(4018..4659)
                     /locus_tag="VYQ93_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV3336200.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4742..4957)
                     /locus_tag="VYQ93_001536"
     CDS             complement(4742..4957)
                     /locus_tag="VYQ93_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV3336201.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4965..5312)
                     /locus_tag="VYQ93_001537"
     CDS             complement(4965..5312)
                     /locus_tag="VYQ93_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV3336202.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5317..6432)
                     /locus_tag="VYQ93_001538"
     CDS             complement(5317..6432)
                     /locus_tag="VYQ93_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV3336203.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6442..7401)
                     /locus_tag="VYQ93_001539"
     CDS             complement(6442..7401)
                     /locus_tag="VYQ93_001539"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV3336204.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7514..8083)
                     /locus_tag="VYQ93_001540"
     CDS             complement(7514..8083)
                     /locus_tag="VYQ93_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV3336205.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
BASE COUNT         2335 a         1592 c         1724 g         2467 t
ORIGIN      
        1 agtagaaacg tttaaaatgt tttcaacaac ctatcgaaat catcgtaaac gcttcggatt
       61 acgaatgaat ttgattgctg gtattatcaa tcatgaacta ggattctagt tttgcaggaa
      121 gtctattgag gtattgagct agtttatgaa aaaattgggt gaaaagtcga gtgttttaga
      181 aacccacagt gtagtattct agtttcaatc cactatattt tgctactccc cgtaaagttt
      241 ctattttccc tgatttctga tataatagaa atattgactt caagagtaag gaagagaaga
      301 tgaacgcatt attaaatgga atgaatgacc gtcaggctga ggcggtgcaa acgacagaag
      361 gtcccttgct aatcatggca ggggctggtt ctggaaagac tcgtgtttta acccaccgta
      421 tcgcttattt gattgatgaa aagctggtca atccttggaa tatcttggcc attaccttta
      481 ccaacaaggc tgcgcgtgag atgaaagagc gtgcttatag cctcaatcca gctactcagg
      541 actgtctgat tgcgaccttc cactccatgt gtgtgcgtat tttgcgtcgc gatgcggacc
      601 atattggcta caatcgtaat tttacaattg tggatcctgg tgaacagcga acgctcatga
      661 aacgtattct caaacagttg aacttggatc ctaaaaaatg gaatgaacga actattttgg
      721 ggaccatttc caatgctaag aatgatttga ttgatgatgt tgcttatgct gcccaagctg
      781 gcgatatgta tacgcaaatt gtggcccagt gttatacagc ctatcaaaaa gaacttcgtc
      841 agtctgaatc cgttgacttt gatgatttga ttatgctgac cttgcgtctc tttgatcaaa
      901 atcctgatgt tttaacctac taccagcaaa aattccaata catccacgtt gatgagtacc
      961 aagataccaa ccacgctcag taccaattgg tcaaactctt ggcttcccgt tttaaaaata
     1021 tctgtgtggt tggggatgcg gaccagtcta tctacggttg gcgtggtgct gatatgcaga
     1081 atatcttgga ctttgaaaag gattacccca aagccaaggt tgttttgttg gaggaaaatt
     1141 accgctcaac caaaaccatt ctccaagcgg ccaacgaggt tattaaaaac aataaaaatc
     1201 gccgtcctaa aaatctctgg actcaaaacg ctgatgggga gcaaatcgtt tactatcgtg
     1261 ccgatgatga gctggatgag gctgtatttg tagccagaac catcgatgaa cttagtcgca
     1321 gtcaaaactt ccttcataag gattttgcag ttctctatcg gactaatgcc cagtcccgta
     1381 caattgagga agccctgctc aagtctaaca ttccttatac catggttggc ggaaccaaat
     1441 tctacagccg taaggaaatt cgcgatatta ttgcttatct caaccttatt gctaatttga
     1501 gtgacaatat tagttttgag cgtattatca acgagcctaa acgtggaatt ggtccaggta
     1561 cagttgagaa aatccgtgat tttgcaaatt tgcaaaatat gtctatgctg gatgcttctg
     1621 ctaatattat gttgtctggt atcaagggta aggcagccca atctatctgg gattttgcca
     1681 atatgatgct tgatttgcgg gagcagctag accacttaag cattacagag ttggttgagt
     1741 ccgtcctaga aaaaacaggt tatgtcgata ttcttaacgc ccaagcgact ctagaaagca
     1801 aggcacgggt tgaaaatatc gaagagtttc tttctgttac gaagaacttt gatgacacca
     1861 ctgatgtgac agaagaggaa actggtctgg acaaactgag tcgtttctta aatgacttgg
     1921 ctttgattgc cgacacagat tcaggtagtc aggagacatc agaagtgacc ttgatgaccc
     1981 tgcatgctgc caaaggtctc gaatttccag ttgtcttttt gattgggatg gaagaaaatg
     2041 tctttccact tagtcgtgcg actgaagatc cagatgaatt agaagaagag cgccgtctag
     2101 cctatgtagg tatcacgcgt gcagagaaaa ttctctatct gaccaatgcc aactcacgct
     2161 tgctttttgg tcgtaccaat tataaccgtc cgactcgttt tattaacgaa atcagttcag
     2221 acttgcttga gtatcaaggt ctggctcgtc ctgcaaatac aagctttaag gcatcatata
     2281 gcagtggtag tatttccttt ggtcaaggta tgagtttggc tcaggctctt caagaccgta
     2341 aacgcggtgc tgccccaaaa tcaatccagt caagcggtct tccatttggt caatttacag
     2401 ctggcgcaaa atcagcatct agcgaggcaa attggtccat tggtgacatt gctctccaca
     2461 agaaatgggg agagggaact gttctggaag tttcaggtag cggtgctagg caggaattga
     2521 aaatcaattt cccagaagta ggtttgaaaa aacttttagc cagtgtggct ccaattgaga
     2581 aaaaaatcta attttccatc cttctcacga ataataaagt gaggaggatt tttatgtaca
     2641 gtatttcatt ccaagaagat tcactattac caagagaaag gctggccaag gaaggagttg
     2701 aagcgcttag taaccaagag ttgctagcta ttttactcag gacaggaaca cgtcaagcta
     2761 gcgtttttga aattgcccaa aaagtcttga acaatctttc aagcctaacg gatttgaaaa
     2821 aaatgaccct gcaggaattg cagagtttgt ctggtattgg gcgtgttaag gccatagaat
     2881 tacaagctat gattgaactg gggcatcgta ttcacaaaca cgagactctt gaaatggaaa
     2941 gtattctcag cagtcaaaag ttggccaaga agatgcagca ggaattaggg gataaaaaac
     3001 aagagcacct ggtggcactc tatctcaata ctcaaaatca aatcatccat cagcagacca
     3061 tttttatcgg ttctgtaact cgtagtatcg ctgaaccgcg agagattctt cactatgcaa
     3121 tcaagcatat ggcgacttct cttatcttgg tccacaatca tccttcagga gcggtagcgc
     3181 ctagccaaaa tgatgatcat gtcactaaac ttgttaaaga agcctgcgaa ctgatgggga
     3241 ttgttctctt ggaccatttg attgtctctc attctaatta ctttagttat cgtgaaaaga
     3301 cagatttaat ctaaagttca ttaacgacat agtcaaagag tttcttatct ttgggacgat
     3361 tttcaaaaag aagttctgga tgccattgga caccgagaaa ggcgacatca tccgtactca
     3421 tgacagcctc aatgatacca tctttaggat catgagccac aacttttaaa tttggtgcta
     3481 agtccttgat gctctggtgg tggaaggagt tgatatgaga gatttctcca tagatttctt
     3541 ggagaacggt atctggttct gttaccaagc gttgagttgt gtactcaaca gaagaatcct
     3601 gccaatggtc ttcgatatct tggtacaaag ttccacccat ggcaacgtta aagagttggg
     3661 taccacggca gacagagaaa atgggctttt tctgtttaat agcttccttg atgagggcca
     3721 gttcgaagat atctctttga aggtgataat catcgctatc aatggttttg ggttcgccat
     3781 agaattttgg atcgacattt tgcccacctg tcaagatgag cttgtcaatc aaactgatat
     3841 agtggcaggc catttcttga tcaccaatcg gtaggatgat aggaatccct ccagcatctt
     3901 taacgccttc aacaaagcct tttgctgcgt agctcatcat gatgtcatca tctggatgag
     3961 ttttttcgtt tcctgtaatc ccaataactg gttttttcat aaaatgattt tcgctttcta
     4021 atcctctttt cgcatgaagt agaggagggt ttggagttca cttgtcaaat cgacatactg
     4081 aacgaccacg tcttttggta aatgcagatg gactggtgaa aaactgagaa ttcctttcac
     4141 accagcatca accaagagat tagcaacctc ttgtgacttg acgctgggaa cagttaggat
     4201 agcagtcttc acatcagcat ccttgatttt atccttgatc tgagaaatcc cgtaaatggg
     4261 aatcccgtca ggagtttggg taccgacttc aggatggtcg tctaggtcaa aggccatgat
     4321 aatcttcatc ttgttacgtt cgtggaagcg gtagtggaga agggcatggc ccatatttcc
     4381 aataccaacc agcatgacat tggtaataga gttgtcattg agcaaatcgg caaaaaatgt
     4441 cattagtttt ttgacatcat agccaaaacc acgacgacca agttcaccaa aataggaaaa
     4501 atcacgacgt acggtcgctg aatcaatacc gatagcctct gcaatttgct tagagttggc
     4561 acgttcaatc ttttctgcat gaaatctctt aaaaattcga tagtagagag agagtctttt
     4621 tgctgtagct tttggaatag caaactgttt atctttcaca aaatcacaac ctttctattc
     4681 ttctatttta tagaaacatt gtgaaaaaat caacaaaaat aagaaaaaac taagaaaaat
     4741 cttagttttg atgtaaaaaa tctgcatgag atagaaaacg gtagaggtct ccgaccagac
     4801 cctgataaac tttttggccc ctaaaagtca gagaagtcac ataaagtgta tctggtaagg
     4861 ttacacatcc tgacaaagtc aacatgagag cctcatgatc ctcatacttg agagtacgct
     4921 ctacatgata gcagtcctta taggtcagtt caaacatttt ggctctatct ttccgatttt
     4981 gtaaagacac cacgttctac caagctatcc atgaggaagt agaatttttc ctgatgaata
     5041 tggtggtctt ctgatttgaa aatatcaact agacgaaggc caaacttgtc agtgatattg
     5101 attttagccc ctgtaagttc cttgttaatg atgattttga gttggaagcc ttcaccgctg
     5161 tttggcactt tttccaaaag gcgagtcagt tcatagttac caaccttagt ttcaaaaaag
     5221 gtgttatctt tgagggtgaa ttttttaaca gaagggctaa gagtgtaatc gtaacgacaa
     5281 ttttttaact gaatgatttt ttcaaatgcc atatggctaa cctccgataa tttcttttaa
     5341 ggtttttgcg agggtttgta ggtcttcaac ggtattttgt ggcgacaaac tgatgcgaag
     5401 ggattccttc aagcgttctg aatttgcgcc atacatggct tcaagaacat ggctggattg
     5461 gacaacgcct gcagtacagg ctgagccagt agagattgaa attccagcta aatctagccg
     5521 aaggagtaag aggtcatttt tctgaccagg aaatccaata ttgagaacat aagggagatg
     5581 atgttttcct ctattcaggt aatactgaat gccctccagc tctgccagaa aggcagtttc
     5641 tagattttgt acatgttgaa aatgttcttc ttgtttttct aggtcttctt ttagggctgc
     5701 aaccatgcct acgatggcag gcagattttc agttcctgca cgttttttct gttcctggtc
     5761 tccgccatgt agataggaat caaagtccat gctagatgcg tagagaaaac cgattccctt
     5821 aggaccatgg aatttgtggg cagaagcagt gagaaaatca atgcccaatt cttctgaatg
     5881 aattgggatt ttaccaatag cctgaactgc atcaacatga taggcagcag ggtgttgctt
     5941 gagtatttgg ccaatttcag cgatgggcag taggtttcct gtctcattat tggcaaacat
     6001 ggtagaaacc aaaatcgtat cgtcacgtaa agccttttga atttgctggg ctgtgatttc
     6061 ttgattttct ggctggataa tggttgcttc aaacccaaag tgttgaacca agtaatcaat
     6121 tgtttcaagg acagcatggt gctcgatggc agttgtgatg atatgttttc cttgttcttg
     6181 gtgacgaaga cagtagccaa tgatggtagt attattgcct tcagtcccac cagaagtgaa
     6241 aaagatatgt tgaggttttg tccttagtaa ctgggctagt tcctgacggg cttctcgcaa
     6301 gagtttgcca gcttgacgac catgaccatg aatactagaa ggatttccgt gggtttcttg
     6361 cataaccttg gtcatagctg aaatagcaac tgctgacata ggagtcgttg cagcattgtc
     6421 caaataaatc aaagaatcac cttatttctt tttattgtag gcaaagagtg ggctgactgg
     6481 ttttctctcg tgaatacgga cgatagcatc accaattaac tcactagcag tgatgtagca
     6541 tacattttta ggagtttttt cttttgttgc tactgaatca gtcacaagaa tttctttaat
     6601 attagtattg tcaagaagct cagcagctcc ctcgacgaag agaccgtggc tagaaacagc
     6661 ataaatttct gtagctcctt cacgttcaac gattttagaa gcttcagaga aggtacgtcc
     6721 tgtatttaaa atatcatcaa tcaagatagc tttcttacct tcaacatcac caataatata
     6781 accttcgtta cgagttgcat cgtcttgagg gtagtcgata atggcgatag gagcatcaag
     6841 atattcagcc aggctacgcg cacgtttgac acctgaattt ttagggctaa cgacaacaac
     6901 atctgaacca agcaatcctt tatcgcagta atgttttgcg aataggggaa cagtgaaaag
     6961 attatccact ggaatatcaa agaaaccttg aacctgaacg gcatgcaaat caagagtcag
     7021 gatacgatca actccagcct taaccagcat attggcaact agttttgctg taagtggctc
     7081 acgaggacaa gcaatgcggt cttgacgtgc atagccaaaa tatggaagga caacgttgat
     7141 actgtgggca cttgcacgca cacaagcatc gaccatgatt aacaattcca ttaggtggtt
     7201 gttgacaggg aaacttgttg attggatgat gtaaacatca taaccacgga cactttcttc
     7261 gatatttact tggatttctc cgtctgaaaa ttgacgtgat gatagttttc caagtgggac
     7321 accaacagct tgggcaattt tttgtgcaat ctcttggtta gagttgagtg cgaaaagttt
     7381 catgtttttt ctatctgaca ttatagaccg tcctctgtaa actttataaa tcctagttat
     7441 atttacctta catatatgaa ctgggatttg tgtattttta tcttttctat tttaccaaaa
     7501 aatggagatt atttcagcta tttttcatac ttttgacaaa tcgaaccaat tttgaaggag
     7561 ctttttggta ggaaatctga tttttctcta aaaattgtcg gaaatcctgt ttgccttgct
     7621 catgattttc cacttcaagc tccaattcgt aatctgttat atcaaagtat cggctctgat
     7681 ccagtgccat gagaccaata gctgttttca tttcatagcg aagcgttgtt agacaaccaa
     7741 gaacctgcca gttcttactt tggataccat gtttcgccaa ttcatccagt actagccctt
     7801 gaggaagttc ttccttactc agatagttct cagcatcttt tagttgcaat ttttggttgt
     7861 attccatgtt tccaacactc tgcgggactt tgagtgtcaa ctcagcccag tcttcaaagg
     7921 ttcgaatgcg catagcgact ttcttttctc gcagttcaaa atcaggcgtg tcgatgtagt
     7981 aatttgtttg aagaacagga gtgacacctg tgaactggtc ttttagacga ttgtattcat
     8041 cttttttcaa tagtgttttc aattcaattt ctaaatgttt catttttctt accttttttt
     8101 atcgttgaaa gcggattt
//
LOCUS       DAVMGZ010000090         8070 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000090 DAVMGZ010000000
VERSION     DAVMGZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.091"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            178..1659
                     /locus_tag="VYQ93_001541"
     CDS             178..1659
                     /locus_tag="VYQ93_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3336206.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRITSQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            2020..3021
                     /locus_tag="VYQ93_001542"
     CDS             2020..3021
                     /locus_tag="VYQ93_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3336207.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3171..4088
                     /locus_tag="VYQ93_001543"
     CDS             3171..4088
                     /locus_tag="VYQ93_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3336208.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            4099..4989
                     /locus_tag="VYQ93_001544"
     CDS             4099..4989
                     /locus_tag="VYQ93_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3336209.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            5018..6634
                     /locus_tag="VYQ93_001545"
     CDS             5018..6634
                     /locus_tag="VYQ93_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3336210.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6644..7963
                     /locus_tag="VYQ93_001546"
     CDS             6644..7963
                     /locus_tag="VYQ93_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV3336211.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDNQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
BASE COUNT         2704 a         1283 c         1534 g         2549 t
ORIGIN      
        1 tgtattcacg ccctcaatag acggtaaaat aagaaaattg tttatatatt gtctccgtag
       61 tgttattata cgaaataaaa gattttagga aaagtcgtta tatcatgcta tacctattct
      121 ttgtggtata attgcaagag gtttaatccg ataatttata aagagagaaa gatattcatg
      181 agagatttat tatctaaaaa aagtcatagg caattagaat tattagaatt attatttgaa
      241 cataaacgtt ggtttcatcg ttctgaacta gcagagttat taaattgtac agaacgtgca
      301 gtcaaagatg atctatccca tgttaaatct gcttttcctg acttgatttt tcattcttct
      361 actaatggta tacgcattat taataccgat gatagtgata ttgaaatggt ttaccatcat
      421 ttctttaaac attcaactca tttttcgatt ttagaattca tcttctttaa tgaaggttgt
      481 caagctgaga gtatttgtaa agaattttat atcagttcat cttcgctcta tcgtattact
      541 agccaaatca ataaagtgat taaaaggcaa tttcaatttg aagtcagtct gacccctgtt
      601 caaatcattg gaaatgagag agacattcgt tacttttttg cacaatattt ttcagaaaaa
      661 tattatttcc tagaatggcc atttgaaaat ttttcatcag agccactatc tcaattgtta
      721 gaattggttt ataaggaaac aagctttcca atgaatttgt caacgcatag aatgctaaag
      781 ttgctcctag ttacgaacct atatagaata aagtttggtc attttatgga agtagataaa
      841 gattctttta acgaccaaag tttggatttt ttaatgcagg cagaaggaat agaaggtgtt
      901 gctcagagtt ttgaatcaga atacaatatc tctttagatg aagaagttgt ttgccaatta
      961 tttgtgtctt actttcaaaa aatgtttttc atagatgaaa gtctctttat gaaatgcgta
     1021 aaaaaggata gttatgtcga aaaatcttac catctattga gtgattttat tgatcagatt
     1081 tcagtcaagt atcagattga aatggagaat aaggataatc tgatttggca tctgcataat
     1141 accgcacatc tgtatcgtca agagttgttt actgagttta ttttatttga tcaaaaaggg
     1201 aatacaatca ggaactttca aaatattttt ccaaaatttg tttcagatat aaaaaaagag
     1261 ctttctcact atttagagac tcttgaggtt tgttctagtt caatgatggt aaatcatcta
     1321 tcttatacct tcatcactca taccaaacat ttggtgatta atttgctaca aaatcagcct
     1381 aaactaaagg ttttagttat gagtaatttt gatcagtatc atgcaaaatt cgttgcagag
     1441 acactttctt attactgtag caataatttt gaactggaag tttggactga attagaatta
     1501 tcaaaggaat ctttagaaga ttcatcttat gatatcatta tttctaattt tattattcct
     1561 cctattgaaa ataagagact catctattca aataatataa acacggtctc actcatatat
     1621 ttgttaaatg ccatgatgtt tattcgatta gatgagtaac caaaaataac aaaaaaacgc
     1681 tatttcgcgt ttgaactgta cctcaaaagt tagacagaaa aaatctaatt tttggggtgt
     1741 ttttgttaag aaaagacctt caatcattta agcaaaatgt acatctagat ttatagccct
     1801 ctaaaactct tgcttttttg tagaagatac gaaaaaagcc ataaaaccaa ggcttttcct
     1861 gttttatgta gatgtttaca catgaaaatt gaagtatttt caaaagctaa ttggagtgat
     1921 tttattcatg atgggataaa tgatctggcc aaaaatgatt gagaaatagt tgcttaaaaa
     1981 gcatttgcat gatactatat attgaaagga ggtgataata tggtagaaca aagaaaatca
     2041 attaccatga aagatgttgc tttagaagca ggagttagtg ttggaactgt ttcacgtgta
     2101 attaataaag aaaaaggcat taaagaagta actttgaaaa aagtggaaca agcgattaaa
     2161 actttgaatt acattccaga ttactacgct agaggaatga aaaaaaatcg aacagaaacg
     2221 attgcaatca ttgtaccaag tatctggcat cccttctttt cagaatttgc tatgcatgtg
     2281 gaaaatgaag tctataagag aaataacaaa ttactcttat gttctatcaa tggtacaaat
     2341 agagagcaag actatctgga gatgttgcgt cataataaag ttgatggagt ggttgccatt
     2401 acctatagtc caattgaaca ttacttgacg tcaggaattc cctttgttag tattgaccgc
     2461 acatactcag atattgccat tccttgtgtt tcatcagata atgatgcagg tggaagagaa
     2521 gcagctaaac aattaattag taaaggatgt cagcatttag cctttgtggg cggacataat
     2581 acaaccatta atgaaaccaa acgtcgtaga atttcatttg aaaagtatgt ccaagagcat
     2641 aaaatacctc ttagtatttt tgatttggat gagacagtta ctgactatta tggaaagcta
     2701 gagagtttct tggaagaaaa ttcttctata gatggaattt ttacaatcaa cgattttaca
     2761 gctttggatg tgattgaaat actagaaaaa aatggcaaac gtattcctca tgatgtccaa
     2821 attattggat acgatggaat taaaatggct ggagatagag attatctact ttcaactatt
     2881 gaacaaccat tggaagaaat ggcaaaagaa gcggttcgta ttttgtttga tataattgat
     2941 ggaaagactg ttaatttgca gacaatccta ccagtaaaat ttgttgaagg aaaaacaaca
     3001 aaaaatgaaa ataagtcttg acagaaagcg ctatcaatga tagaatgaat tcagataaaa
     3061 agatttattt ttaaaacaaa aatgaaacgt ttcaaaaaaa gaaataaaga gacagcgcca
     3121 agcgctatct tttctagaaa aaaatgaaac gtttcaaaaa ggaggttgct atgaatagca
     3181 aagcgaagca agtttctctt tgggaaagaa tcaagaaaca aaaactcttg ttattgatga
     3241 ctgtccctgg tttagtttta acctttatct ttaaatacat ccctatgtat ggggttttaa
     3301 ttgcatttaa agattacaat cctttaaaag gaattttagg gagtgattgg attggttttt
     3361 ctgagtttac aaaattcata tcctctccca actttggtat cttgttagcc aacacattaa
     3421 aattaagtat ctatggttta ttgcttggct ttttaccacc aatcattctt gctattatgc
     3481 tcaatcaact cttgagtgaa aaagtcaaaa aacgaattca gctcatttta tacgcaccaa
     3541 actttatctc agttgttgtt attgtcggta tgattttcct cttcttttca gtgggaggac
     3601 caatcaacaa ttttctttct atgtttggaa tgaaggctga cttcttgaca aatccagact
     3661 tctttagacc tttatacatc tttagtggta tctggcaagg aatgggctgg gcttcaacgc
     3721 tctacacggc aacattggta aatgtagatc cagccttagt agaagcagcc cgactggatg
     3781 gagccaatat cttccaacga atctggcaca ttgatattcc agctcttaag cctattatgg
     3841 ttatccaatt tgttttagct gcaggtggaa ttatgaatgt cggatatgaa aaagcattct
     3901 tgatgcagac atcgttaaat ttgccaactt ctgaaattat ctcgacatat gtctataaag
     3961 ttggtcttgt atcaggagac tattcttact caacagcggt tggtttgttt aatgcagtga
     4021 ttaacgtagt attgcttgtt gcagttaacc aaatcgttaa acgcatgaat aatggtgaag
     4081 gaatttaagg aggaaagtat gaaaaattcg attatggata caaaatttga tagacgtatc
     4141 ttactcttaa ataaaatcat tattgtcttt atcgttttga tgactttgct tcctttactt
     4201 tatatcgtcg tagcatcctt tatggatcct aaggttctgg ttagtagagg gattagcttt
     4261 aatccagccg attggactgt agaaggttac cagcgtgtat tcagtgacca atctattcta
     4321 agaggtttta tcaattctct actatactct tttggatttg cagctttaac agtattgcta
     4381 tctgtgttta cagcttatcc tctttctaag aaagacttgg ttggacgtcg ttggattaac
     4441 tacttcttga ttgtgactat gttctttggt ggtggtttag tcccaactta cttgctcgta
     4501 aaagaattgg gaatgctcaa tactccatgg gctatcattg ttccaggtgc tgtcaacgtt
     4561 tggaatatta ttcttgctag ggcctatttc caaggattgc ctgaagaatt agttgaagct
     4621 gctgtcattg atggtgcaaa tgatttacag attttcttca aaatcatgct tcctcttgca
     4681 aaaccaatta tgtttgttct cttcctttat gcttttgtag gacagtggaa ctcatacttt
     4741 gatgcaatga tttatatcaa ggatccaaac ttggaaccat tgcaacttgt acttcgtaaa
     4801 attctcattc agagccaacc aggtcaagac atgattggag cacaagcggc tatgaatgaa
     4861 atgaaacgtt tagctgaatt gattaaatac gcaactattg tcatttccag cttgccattg
     4921 attgttatgt atccattctt ccaaaaatac tttgataaag gaattatggc tggttcactt
     4981 aaaggataaa aaaagaaaaa ataaaaggag ttttctcatg aaattcaaaa cattctcaaa
     5041 atcagcagtt ttgttgacag ctagtttagc agtacttgca gcctgtggct caaaaaatac
     5101 agcttcaagt ccagattata agttggaagg tgtaacattc ccgcttcaag aaaagaaaac
     5161 attgaagttt atgacagcca gttcaccgtt atctcctaaa gacccaaatg aaaagttaat
     5221 tttgcaacgt ttggagaagg aaactggcgt tcatattgac tggaccaact accaatccga
     5281 ctttgcagaa aaacgtaact tggatatttc tagtggtgat ttaccagatg ctatccacaa
     5341 cgacggagct tcagatgtgg acttgatgaa ctgggctaaa aaaggtgtta ttattccagt
     5401 tgaagatttg attgataaat acatgccaaa tcttaagaaa attttggatg agaaaccaga
     5461 gtacaaggcc ttgatgacag cacctgatgg gcacatttac tcattcccat ggattgaaga
     5521 gcttggagat ggtaaagagt ctattcacag cgtcaacgat atggcttgga ttaacaaaga
     5581 ttggcttaag aaacttggtc ttgaaatgcc aaaaactact gatgatttga ttaaagtcct
     5641 agaagctttc aaaaacgggg atccaaatgg aaatggagag gctgatgaaa ttccattttc
     5701 atttattagt ggtaacggaa acgaagattt taaattccta tttgctgcat ttggtatagg
     5761 ggataacgat gatcatttag tagtaggaaa tgatggcaaa gttgacttca cagcagataa
     5821 cgataactat aaagaaggtg tcaaatttat ccgtcaattg caagaaaaag gcttgattga
     5881 taaagaagct ttcgaacatg attggaatag ttacattgct aaaggtcatg atcagaaatt
     5941 tggtgtttac tttacatggg ataagaataa tgttactgga agtaacgaaa gttatgatgt
     6001 tttaccagta cttgctggac caagtggtca aaaacacgta gctcgtacaa acggtatggg
     6061 atttgcacgt gacaagatgg ttattaccag tgtaaacaaa aacctagaat tgacagctaa
     6121 atggattgat gcacaatacg ctccactcca atctgtgcaa aataactggg gaacttacgg
     6181 agatgacaaa caacaaaaca tctttgaatt ggatcaagcg tcaaatagtc taaaacactt
     6241 accactaaac ggaactgcac cagcagaact tcgtcaaaag actgaagtag gaggaccact
     6301 agctatccta gattcatact atggtaaagt aacaaccatg cctgatgatg ccaaatggcg
     6361 tttggatctt atcaaagaat attatgttcc ttacatgagc aatgacaata actatccaag
     6421 agtctttatg acacaggaag atttggacaa gattgcccat atcgaggcag atatgaatga
     6481 ctatatctac cgtaaacgtg ctgaatggat tgtaaatggc aatattgata ctgagtggga
     6541 tgattacaag aaagaacttg aaaaatacgg actttctgat tacctcgcta ttaaacaaaa
     6601 atactacgac caataccaag caaacaaaaa ctagaggttg attatgggag ataagaaata
     6661 cacagtagaa aaagccaatc gttttatagc agaaaataaa catctcgtta atactcaata
     6721 taagcctgaa gaacattttt cagctgagat tggttggatc aatgatccaa atggatttgt
     6781 ctattttcgt ggagaatacc atctctttta tcaattctat ccatatgata gtgtttgggg
     6841 gcctatgcac tggggacatg ctaaaagtaa ggacttggtg acttgggagc acttgccagt
     6901 ggcacttgct cctgaccaag attatgaccg aaatggttgt ttctcaggct ctgccattgt
     6961 caaggatgat cgcctctggc tcatgtacac tgggcatatc gaagaaaaaa ccggtgtccg
     7021 ccaagtgcaa aatatggcat tttcagatga cgggattcac tttgaaaaga tttcccaaaa
     7081 tccagttgta actggaacag acttgccaga tgagttgatt gctgctgatt tccgtgaccc
     7141 aaaactcttt gaaaaagatg gacgctatta ctccgtagta gctgccaaac acaaggataa
     7201 tgtgggctgt atcgttctac tagggtccga taacctagta gaatggcagt tcgaatccat
     7261 ctttttaaaa gggggagaac accaaggctt tatgtgggaa tgcccagatt acttcgagtt
     7321 agatgggaaa gattgcctta ttatgtcacc catgcgttat cagcgtgagg gagactcata
     7381 tcataacatc aactcatcgc ttttgttcac gggtaaggta gattggggag aaaaacgttt
     7441 tatcccagaa tcagttcaag aaattgatca tggccaagac ttctatgcgc ctcaaacatt
     7501 gttggacaat caaaatcgtc gtatcctgat tgcttggatg cagacatggg ggcgtaccct
     7561 tccaacccat gaccaagaac acaaatgggc atgtgccatg actttaccta gaattctaag
     7621 attggaagat ggcaaactaa gacaattccc tgttaaaaaa ggccaatatc aaatccaaat
     7681 agataaagat tgtcattacc acttaggaaa tgatatagat tatcttgaat ttggttatga
     7741 cagtaatgcg cagcaagttt acattgatcg tagccatctt attcaaaaaa ttctaggtga
     7801 agaagaacag gacactagtc gacggtatgt agatattgaa gctaaagaat tggaagttgt
     7861 tctagataaa aattccatcg agatttttgt caatcaaggt gaagcaagct tgactgcaac
     7921 ttattactta acggtgccag ctgagctatc acgaattgat taaaaattaa gttatttctc
     7981 ctaaagaaaa agttctcttt ctaaaatagt ggaaagagga ctttttgtgt tttgggtata
     8041 taagcttagt ttatggtatt tgtaaaattg
//
LOCUS       DAVMGZ010000091         8057 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000091 DAVMGZ010000000
VERSION     DAVMGZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.092"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            142..723
                     /locus_tag="VYQ93_001547"
     CDS             142..723
                     /locus_tag="VYQ93_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV3336212.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            720..1997
                     /locus_tag="VYQ93_001548"
     CDS             720..1997
                     /locus_tag="VYQ93_001548"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV3336213.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYCPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            2015..2677
                     /gene="deoC"
                     /locus_tag="VYQ93_001549"
     CDS             2015..2677
                     /gene="deoC"
                     /locus_tag="VYQ93_001549"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV3336214.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            2664..3053
                     /locus_tag="VYQ93_001550"
     CDS             2664..3053
                     /locus_tag="VYQ93_001550"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV3336215.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            3143..4195
                     /locus_tag="VYQ93_001551"
     CDS             3143..4195
                     /locus_tag="VYQ93_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV3336216.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            4288..5823
                     /locus_tag="VYQ93_001552"
     CDS             4288..5823
                     /locus_tag="VYQ93_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336217.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            5816..6874
                     /locus_tag="VYQ93_001553"
     CDS             5816..6874
                     /locus_tag="VYQ93_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3336218.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KEFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            6877..7833
                     /locus_tag="VYQ93_001554"
     CDS             6877..7833
                     /locus_tag="VYQ93_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3336219.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            7918..>8057
                     /locus_tag="VYQ93_001555"
     CDS             7918..>8057
                     /locus_tag="VYQ93_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3336220.1"
                     /translation="MKLSCDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKLIDR
                     YG"
BASE COUNT         2287 a         1444 c         1927 g         2399 t
ORIGIN      
        1 ggtttattat ttataagtgt tgaaagagat tgaagaaaat ggcttcgtga ctcgctttct
       61 tcggtctttt tcgttttatt atcaatttgt aaacgattta caatttgagt caaattatgg
      121 taaaatagtg tcatgagtaa aatgtattat gcagaaaatc ctgacgctgc tcacgacatt
      181 catgagttga gagtagagtt gttgggacaa aaaatgacct ttttaacgga tgcgggtgtt
      241 tttagcaaga aaatagttga ctttggaagt caactcttgc tcaagtgtct tgaggtcaac
      301 caaggagaaa ctgtccttga tgtaggctgt ggttacggac ctttgggttt gtccttagct
      361 aaggcttatg gagttcaggc tactatggtt gatatcaaca ctcgtgcctt ggatttagct
      421 cggagaaatg ctgaaaaaaa taatgcaaaa gcgacgattt tccagtccaa catctatgaa
      481 caagttgagg gacattttga tcatgtcatt tccaatcctc ctatccgtgc gggcaagcaa
      541 gtcgttcatg aaatcattga gaaaagtaaa gatttcttgg aaactggtgg ggatttgaca
      601 atcgtcatcc agaaaaaaca aggggctcca agtgctaagt ccaagatgga ggatgtgttt
      661 ggtaattgtg aaatccttaa aaaagacaaa ggatactata tccttagaag tgtgaaagaa
      721 tgagagcagt tgatttaata caaaaaaaac gagacggtca agaactgact tcaagtgaaa
      781 ttgaatggct gatagaaggc tatgtgtcag gaactgttcc tgattatcag atgtctgcct
      841 ttgctatggc tgtttatttt aaaggaatga cgactcgtga aatttctgat ttaacgatga
      901 atatggttaa gactgggcaa gagtttgacc tgtcagccat tgatggtgtg aaagttgaca
      961 agcattctac tggtggtgtc ggcgataagg taaccttgat tttagctcct cttgttgcta
     1021 gcttcggtgt gcctgtagca aaaatgagtg gtcgtggtct tggccataca ggcggaacaa
     1081 ttgataaatt ggagtccatt aagggctatc aagtagaacg tagtcaagag gattttattc
     1141 gtcaggttca ggatattgga gtatctgtca ttgggcaatc agaccagctg gtcaaagcag
     1201 ataagcttct ctatgccctt cgtgatgtga ccgcaactgt cgacacgatt cctttgattg
     1261 cgagttcggt gatgagcaag aaaattgcgg caggggcgga tgctattttg ctagacgtga
     1321 ctgtcggtga gggtgccttc atgaagacgg ttgatgaggc gcgtgagttg gctcaaacta
     1381 tggtagatct tggtaaggtt gttggacgga agacagtagc agtgattacg gatatgagcc
     1441 agcctttggg acgagcgatt ggaaatcgtc tggaaatcct tgaagcattg gagattttgc
     1501 aaggtcaagg acgtcaggat attacccact ttatttgtga attggctcaa attatgcttg
     1561 gtttggcaaa tgttaacaaa actgttgaag aagttcgcca acatcttgag aatggtcaag
     1621 cactggctaa gtttgaggaa atggtgcaag cccaaggtgg agacttggaa gacctctatt
     1681 gtcctgtaaa tgtagctcat gtggtggaaa tccctgctca ggaaacgggt gtcatttcag
     1741 ctcttccagc tatggacttt ggtctttatg ccatgaggct gggtgctggt cgtgcagtta
     1801 agtctgatgc cttggactat gaaacaggaa ttgtttttga aaagaagatt ggagactccg
     1861 ttcaaaaggg tgaaattgtt gcaaaagtat acacaaatga aaaaattcca cctcaactag
     1921 ttacagattt ccaaaaatgt gttaaaataa gtgatgaagt gaaaaaaatc cgtgaaattg
     1981 tagaaatcat ctcctaaatg gagggaaaac cgaaatgaaa ttaaataaat atatagatca
     2041 tacgctttta aaacaagatg caaagaaaaa acaaattgat agtttgttgt ctgaggctag
     2101 agagtatgat tttgccagtg tttgcgttaa tccgacctgg gttgaacatg ctaaaaaagg
     2161 acttgaaggc acagatgtta aggtttgcac agtagtaggt ttccctttgg gagcaacaac
     2221 ttcagccgtg aaagcatttg agacaaaaga agctatccaa aatggtgcag atgagattga
     2281 tatggtgatc aatgttggag ctctcaaatc aggtaattta gccttggttg agtcagatat
     2341 tcgcgcagta gtggaagcaa gtggtgataa gttagtgaaa gtcattattg aagcttgcct
     2401 tctgacagac caagaaaaaa ttgttgtttg ccaattggcc caaaaagctg gggctgactt
     2461 tgttaaaaca tctactggtt tttcaactgg tggtgctacg atagcagatg ttacattaat
     2521 gcgtgaaaca gttggatctg atatgggagt caaggctgct ggtggagctc gttcttatgc
     2581 agatgctctt gcctttgtcg aagcaggtgc gacccgtatc ggaacgtcag ctggggtagc
     2641 tattttaaaa ggagaattgg cagatggcga ctactgagtt gattgaactg gcaattgaaa
     2701 ccagcaaaca tgcctatgtc ccctattctc actttcctat cggagcagtt ttagtagcca
     2761 aagacggaag tgtttacacg ggagtgaata tcgaaaatgc tagctatcct ttgaccaatt
     2821 gcggtgagag aacagctgtt tttaaggcta tatctgaagg ccaaagagaa ttttcagaat
     2881 tgattgtcta tggtcaaact gaaaaaccga tctcgccatg tggtgcttgt cgccaagtaa
     2941 tggtcgaatt ttttgaacaa gatttaaaag tgaccttagt cgcaaaagat aaatcgacgg
     3001 tcgagatgac ggtcggggaa ttacttccat actcttttac agacttaaac tagtctgagt
     3061 cgctctctga gtggcacggt ccttgtggcc aaccaatcca tacttgcaac atcgttgcac
     3121 atcttattta ggaggttcag taatgaacaa gaaacaatgg ctaggtcttg gcctagttgc
     3181 agtggcagca gttggacttg ctgcatgtgg taaccgctct tctcgtaacg cagcttcatc
     3241 ttctgatgtg aagacaaaag cagcaatcgt cactgatact ggtggtgttg atgacaaatc
     3301 attcaaccaa tcagcttggg aaggtttgca ggcttggggt aaagaacaca atctttcaaa
     3361 agataacggt ttcacttact tccaatcaac aagtgaagct gactacgcta acaacttgca
     3421 acaagcggct ggaagttaca acctaatctt cggtgttggt tttgcccttc acaatgcagt
     3481 tgaagaagca gctaaagaac acactgactt gaactatgtc ttgattgatg atgtgattaa
     3541 agaccaaaag aatgttgcga gcgtaacttt cgctgataat gagtcaggtt accttgcagg
     3601 tgtggctgca gcaaaaacaa ctaagacaaa acaagttggt tttgtaggtg gtatcgaatc
     3661 tgaagttatc tctcgttttg aagcaggatt caaggctggt gttgcgtcag tagacccatc
     3721 tatcaaagtc caagttgact acgctggttc atttggtgat gcggctaaag gtaaaacaat
     3781 tgcagccgca caatacgcag ccggtgcaga tattgtttac caagtagctg gtggtacagg
     3841 tgcaggtgtc tttgcagagg caaaatctct caacgaaagc cgtcctgaaa atgaaaaagt
     3901 ttgggttatc ggtgttgatc gtgaccaaga agcagaaggt aaatacactt ctaaagatgg
     3961 caaagaatca aactttgttc ttgtatctac tttgaaacaa gttggtacaa ctgtaaaaga
     4021 tatttctaac aaggcagaaa aaggagaatt ccctggcggt caagtgatcg tttactcatt
     4081 gaaggataaa ggggttgact tggcagtaac aaacctttca gaagaaggta aaaaagctgt
     4141 cgaagatgca aaagctaaaa tccttgatgg aagcgtaaaa gttcctgaaa aataatggat
     4201 ggaagtcatt ttgtctcagt aaagcttgct actagacaaa ctagcaagtt ttattgaaat
     4261 aaaataagac tctgaaagga agagcacatg gcacacgaaa atgtcattga gatgcgtgat
     4321 attaccaagg tgtttggtgg atttgttgcc aacgacaaaa tcaacttgca cctacgaaaa
     4381 ggtgaaattc atgcactttt aggagaaaat ggggctggta agtccacgct aatgaacatg
     4441 ttagcaggcc ttcttgaacc aactagtggt gaaatcgcgg tcaacggtca agttgtcaat
     4501 ctcgactccc catctaaagc agctagcttg ggaatcggga tggttcacca gcactttatg
     4561 ttggttgaag ccttcacagt ggctgaaaac atcattttag gtagtgaatt gactaaaaat
     4621 ggtgtgctag atatcgctgg agctagcaaa gaaatcaagg ctctttctga acgttatggc
     4681 ttagctgttg acccttctgc caaggtagca gatatctcag ttggagccca acaacgtgta
     4741 gaaattttaa aaacacttta taggggggct gatatcctta tctttgacga accaacggct
     4801 gttttgactc catcagaaat tgatgagttg atggctatta tgaaaaatct tgtcaaagaa
     4861 ggaaaatcaa ttatcttgat tacccacaaa ttggatgaaa ttcgcgcagt ttctgaccgt
     4921 gttacagtta tccgtcgtgg gaaatcaatt gaaaccgttg aaattgcagg ggctaccaat
     4981 gctgatttgg cggaaatgat ggtaggacgt tctgtttcct ttaaaacaga gaagcaagcc
     5041 tctaaaccaa aagaagtggt tttgtctatc aaagatttgg tggtcaatga aaaccgtggt
     5101 gttccagctg ttaaaaatct gtccttggat gttcgtgctg gagagattgt tggtattgcg
     5161 gggattgatg gaaatggtca gtctgaactg attcaagcca ttacaggtct tcgtaaggtt
     5221 gaatctggta gcattgagct aaaaggagat tcaattgtag gcttgcaccc acgtcagatt
     5281 acagaactaa gtgttgggca cgttccagaa gaccgtcacc gtgatggctt gattttggaa
     5341 atgatgatat ctgaaaatat tgcccttcaa acctactata aagaaccaca tagtaaaaat
     5401 ggaattttga attattcaaa tattacttct tatgctaaaa agctgatgga agagtttgat
     5461 gttcgcgctg ccagtgaatt agttcctgca gctgcactct caggaggaaa tcaacaaaaa
     5521 gcaattattg ctcgtgaaat tgatcgagat cctgatctcc ttatcgttag ccagccaact
     5581 cgtgggttgg atgtcggtgc cattgagtat atccacaaac gcttgattga agagcgtgat
     5641 aatggcaagg ctgtccttgt tgtcagcttt gaattggatg agattttaaa cgtctcagac
     5701 cgtattgccg ttatccacga tggtaagatt caaggtattg tatcaccaga aacaaccaat
     5761 aaacaagaac ttggtgtctt gatggctggt ggaaacttgg gaaaggagaa gagtgatgtc
     5821 taaaaaatta caacaaattt cggttccctt gatttctgta tttctaggaa ttttactcgg
     5881 agccattgtc atgtggatct tcggttatga tgctatttgg ggctacgaag aattgttcta
     5941 tacagccttt ggcagtctgc gtgggattgg agaaatcttc cgtgctatgg gtcctctggt
     6001 cttgattggt cttggttttg cggttgccag tcgagctggt ttctttaacg tcggacttcc
     6061 tggtcaggct ttggcaggtt ggattctcag tggttggttt gcgctgtcgc atccagatat
     6121 gccccgtccc ttgatgattc tagcaaccat cgtgattgcc ttgattgctg gtgggattgt
     6181 cggagcgatt ccaggtattc ttagggccta tctagggacg tcagaggtta ttgtaaccat
     6241 catgatgaac tacattgtct tgtatgtagg gaatgccttt atccatgctt tccctaaaga
     6301 attcatgcaa agtacagatt cgaccattcg tgttggggct aatgcaacct atcagacacc
     6361 ttggttggct gagttgactg gtaactcacg gatgaatatt ggtattttct ttgccatcat
     6421 tgccgttgca gttatttggt tcatgctcaa gaaaacaact cttggttttg aaatccgtgc
     6481 agttggtctt aatccacatg cttcagaata tgctggtatt tctgccaagc ggactattat
     6541 cctatctatg attatttcag gtgccttggc aggtcttggt ggagctgttg aaggtttggg
     6601 aaccttccag aacgtctatg ttcaaggttc gtcattagct atcggattta acggaatggc
     6661 ggttagtttg cttgcggcca actcaccaat tggtatactc tttgcagcct tcctatttgg
     6721 cgttctccaa gttggggctc ctggtatgaa tgcggcgcag gtaccatctg agcttgtcag
     6781 cattgtaaca gcgtctatta tcttctttgt cagtgttcat taccttatcg aacgctttgt
     6841 caaaccgaaa aaacaagtta aaggaggtaa gtaaagatgt ctataataac cttgctcccc
     6901 ctcttggtgt cttctatgct gatttactca gcacctctca tctttacaag tatcggtggt
     6961 gttttctctg aacgtggtgg tgtggtaaac gttggccttg aaggaattat ggttatgggt
     7021 gccttttctg gagttgtctt taaccttgaa tttgcagaac aatttggagc agcaactcca
     7081 tggctatcct tgcttgtagc aggattggtt ggtagtgttt tctctatcat ccacgcagca
     7141 gcgacggttc atttccgtgc agaccatgtt gtcagcggta cggtattgaa cttgatggcg
     7201 cctgccttgg ctgttttctt agttaaagtt ctttataaca aaggacaaac cgacaaccta
     7261 agtcaaactt ttggacgctt tgatttccca gtcttggcaa atatcccagt gattggtgat
     7321 atcttcttca agtcaactag tctacttggt tatctggcga ttgccttctc attccttgct
     7381 tggtttattc tctttaagac tcaatttggt cttcgtctcc gctctgtcgg tgaacatcct
     7441 caagcagcgg ataccttggg aatcaacgtc tacaagatga gatatttagg gattattatt
     7501 tcaggttttc taggtggaat tggcggagcg atttatgcgc aatcaatctc agttaacttc
     7561 tcagtgacaa ctattgttgg acctggattt atcgcccttg ctgcgatgat ctttgggaaa
     7621 tggaatccaa tcggtgctat gctttctagt ctcttctttg gactttcaca aagtttggct
     7681 gttatcggtt ctcaattgcc gttcctacaa ggagtgccag cggtttatct tcaaattgca
     7741 ccttatgttt tgacaattct tgtcttagca gccttctttg gaaaagcagt cgcaccaaaa
     7801 gcagatggta tcaactacat caaatcaaaa taagcataca aaaaaacgtc agtccaatga
     7861 ctgaactgcc ccccaaaagt tagacagaaa aaatctaact tttggggtgt ttttattatg
     7921 aaattgagtt gtgatgataa agttcagatc tatgaactta gaaaacaagg atatagctta
     7981 gagaagcttt caaataaatt tgggataaac aattctaatc ttaggtatat gattaaattg
     8041 attgatcgtt acggaat
//
LOCUS       DAVMGZ010000092         7655 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000092 DAVMGZ010000000
VERSION     DAVMGZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.093"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..149
                     /locus_tag="VYQ93_001556"
                     /pseudo
     CDS             1..149
                     /locus_tag="VYQ93_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(286..1254)
                     /locus_tag="VYQ93_001557"
     CDS             complement(286..1254)
                     /locus_tag="VYQ93_001557"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV3336221.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALSEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1399..2214)
                     /locus_tag="VYQ93_001558"
     CDS             complement(1399..2214)
                     /locus_tag="VYQ93_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEV3336222.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPD
                     STFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLI
                     FLGLALGTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2239..2736)
                     /locus_tag="VYQ93_001559"
     CDS             complement(2239..2736)
                     /locus_tag="VYQ93_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV3336223.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2809..4170)
                     /gene="radA"
                     /locus_tag="VYQ93_001560"
     CDS             complement(2809..4170)
                     /gene="radA"
                     /locus_tag="VYQ93_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV3336224.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4184..4699)
                     /locus_tag="VYQ93_001561"
     CDS             complement(4184..4699)
                     /locus_tag="VYQ93_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV3336225.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4701..5144)
                     /locus_tag="VYQ93_001562"
     CDS             complement(4701..5144)
                     /locus_tag="VYQ93_001562"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV3336226.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            5449..5598
                     /locus_tag="VYQ93_001563"
     CDS             5449..5598
                     /locus_tag="VYQ93_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336227.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            5740..5919
                     /locus_tag="VYQ93_001564"
     CDS             5740..5919
                     /locus_tag="VYQ93_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336228.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(6139..6504)
                     /locus_tag="VYQ93_001565"
                     /pseudo
     CDS             complement(6139..6504)
                     /locus_tag="VYQ93_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            6524..6691
                     /locus_tag="VYQ93_001566"
     CDS             6524..6691
                     /locus_tag="VYQ93_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEV3336229.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            complement(6846..7049)
                     /locus_tag="VYQ93_001567"
     CDS             complement(6846..7049)
                     /locus_tag="VYQ93_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336230.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(7072..7263)
                     /locus_tag="VYQ93_001568"
     CDS             complement(7072..7263)
                     /locus_tag="VYQ93_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV3336231.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
BASE COUNT         2375 a         1767 c         1339 g         2174 t
ORIGIN      
        1 ctgagtgcgg aaaccaattg aagaaatatg actttcaaaa accttctaaa attccttatc
       61 ttgaaacgac tggtatacct actagaattc tccttagaaa gcgtcgattc aagtgctatc
      121 actgttcaaa aatgatggtc gctgaaactt ctgatgacgt acagtcatat ttcttctctt
      181 tttattatat cacagtttta aatctagctt tactagattc accgctacta tctatttatt
      241 cggaaaaaag acgaaaaaac ctgagaatca tctcaggctt ggtcattaaa tttttttctc
      301 aatatcgaaa agtggagaaa gtggtcgttt ttcatgaata cgtacgatag catcccctag
      361 gagatgagcg attgaaatct gctcaatctt atcaatcaaa cgctcttctg gcagatagat
      421 ggtatccaaa acaaccaatt tcttaatagc tgatttttgg atattgtccg tagcaggacc
      481 agaaagaact gggtgcgtac agcttgcata gacttcaaca gcaccagctt ccgaaagagc
      541 atctgccgca tgacaaatcg ttccagcggt atcaatcata tcatcaatca agatacaagt
      601 cttgccttca accttaccga tgatgttcat aacttcacta gtattcatct tatcaacgct
      661 acgacgttta tcaataatag cgatagatgt tttcaaaaat tctgccaact tacgagcacg
      721 agtcacccct ccatggtccg ggctgacaac cacatagtca gaaccaacca taccacgacg
      781 ctcaaaataa tctgcaatca gaggagcacc catcaaatga tccacaggaa tatcaaagaa
      841 tccttgaatt tgcgcagcat gcaagtcgat ggtcaataaa cgatccactc cagctacttc
      901 aagcatattt gcgacaagtt ttgaagtgat tggctcacgc gctctcgcct ttctatcctg
      961 acgtgcatac ccatagtaag gcatgacaac attgacagat tctgcactcg cacgcttcaa
     1021 agcatctacc ataatcaaaa tttcaagcag attgtcattt acaggcgaac tagttgattg
     1081 taggataaag acgtgtttcc cacggattga ttcttcaatg ttgacctgaa tctctccatc
     1141 tgaaaattgg cgaacacttg atttccccaa ctctatccca atctcctgcg ctacacgttc
     1201 tgccaattct ttattagaag aaagggcaaa cagctttaaa tcagaaaaag acatgatttc
     1261 ctccggtata tatgtataac ttgtgctttt cacaagattt tccatctacc attgtagcgc
     1321 tttttgcact atttttcaat caaaaataaa agaagggcac catatttgta cccttgcatc
     1381 attcttttga aaaatattct aggtcatcaa ctcattgtgt ttctcaacaa agcaataagc
     1441 atgataaaaa ccatagagag caatagccgt aaccactgga atcgctaaag gcaactctgt
     1501 ttccaactcc acaaaaggag agttaaacaa gaagtgagtt cccaaggcta aacctagaaa
     1561 aataaggccc tgtttcttgc caaccttctg tcctttatag gctctgtaaa gcaagtaaac
     1621 acctactaca gctagacctg aaaaagtcca gtgagaggca attcctgaga tgatacgctc
     1681 taaaattcgc gaaatagtaa agtcaaagcc ctctggcaaa tccgtacgaa tataaccaat
     1741 atcctcaatc atttggaatc ccaaaccgga agcaattcca agtaaaaaca aagattttaa
     1801 ttttcgcaca ggaatcaaag ccaaaacaaa aacaagtggc aataatttca agggttcttc
     1861 taccaaagga gccacaatag cactttcaaa ggcatttaaa aatgtactat ctgggaaaag
     1921 aacccccagt aaatcatgta tataagtatt agcaaaacta gacaaccagc ctgaaaggaa
     1981 catccctccc aataaagaca gaatcaaaac cttctttggc aattcccatt ttttcccaat
     2041 acggaagaga aaataaagag ccggaatcat gtaaaagaga gctagaaaga tagaaactcc
     2101 cattagtcca tattccgcac ctgacctcga accgtccgta tagtagatgg tttcatactg
     2161 taaaccaata catagcaata aaataaaaat aaataaaata ctgcttttct tcatacactt
     2221 tctttctaaa tgaagtattt ataattctac gactgtcata cttcctgtat caacattgta
     2281 aatagcacca gagataatga catcgtctgg tattagggga gactcgataa gcagttgcat
     2341 atcctcgcgt acactctctt ctatatcttg gaagggcaag aagtcctggt ctgacacatc
     2401 tacacctaat tcctctttta aatactcctg aaaaggttca ttttcaaagg tctgagcacc
     2461 acagtctgta tggtgcaata ccacaatctc tcttgtcccc atttgttgct gggaaataac
     2521 tagcgaacga atcatgtctt cagtcactcg accacctgca ttccgcaaga tatgagcatc
     2581 cccaagtgcc aagcccagag cttgcgcaac gtgcaaacgt gagtccatac aggtcacaat
     2641 ggctactctg gttttaggtt taagtggcag atttaactgc ccatgtaggg caacataagc
     2701 ctgattggct tgcataaact gttcaaaata cgacacgatt ccctccttga aaatttgata
     2761 gtcaaatatt tctcctatct tatcattttt aagagaattt gtcacggatt atgcaaagac
     2821 ctttttcaag acttcctgaa tcgttgtcac gccaatgacc tgaatttcct taggcagagt
     2881 gattcctgtc aaggaattct gaggtacata aatcttagta aagcccagtt tagcagcttc
     2941 gttgatgcgt tgctcaatac gattcacgcg ccgaatctct cctgtcaagc ccagttctcc
     3001 gacaaaacat tcctgaggat tagttggctt gtctttgtag ctcgaagcaa tagcaactgc
     3061 aacagccaag tcaatggcag gttcatccaa tttaacacca ccagcagatt tgagataggc
     3121 atcctgattt tgcaagagaa gccctgcccg tttttccaaa acagccataa tcaagctagc
     3181 acggttaaaa tcaagtcctg tcgtagtacg cttggcattt ccaaacatgg tcggtgttac
     3241 caaagcctga acctccgcca aaatcggacg cgtcccttcc atggttacaa cgatggagga
     3301 accagtcgcc ccatccaaac gctcttctag gaaaacttga ctcggattga gtacctcaac
     3361 caagccgccc gactgcatct caaaaatccc aatctcatta gtggaaccaa aacgattttt
     3421 gaccgctctc aaaatacgaa aggtgtggtg acgctcccct tcaaagtaaa gcaccgtatc
     3481 caccatatgc tccaacatac gaggcccagc caaggttcct tctttggtca catgacctac
     3541 gataaagatg gcaatgttat tggtcttggc caactgcatg agttcagcgg tcacttcacg
     3601 tacctgagaa acagacccct gcacccctga aatctcagga gacatgatgg tctggatgga
     3661 atcaataatg agaaagtctg gctggatacg ctccacttct gcacgaacac tctgcatatt
     3721 ggtctctgca tagagataaa actcactatc aatatcacct aagcgctctg cacgtagttt
     3781 aatctgctgg gcagactcct ccccactgac atagagaact gtccccactt gggacaactg
     3841 ggttgagact tgtaggagaa gagttgattt cccaatccca ggatccccac cgatgaggac
     3901 gagacttcct ggtaccactc cgcctccaag cacacggttg aattcctcca tctccgtctt
     3961 ggttcgattg acattgatgg aagtcacctc agctagtttc atgggcttgg ttttctcacc
     4021 tgtcaaggac acacgcgcat tcttgacctc ggcaacctca acctcttcca caaaagaaga
     4081 ccaagaccca cagttggggc aacgtcccag atatttaggg gaattatacc cacaattttg
     4141 acatacaaat gtcgcttttt tctttgcgat gacaaacctc tttctatatc tctaactcac
     4201 actcaatcac ttggcaaaaa tcaatcttct catttggcac aaactggcgc atgagcattc
     4261 gatgagcaac aactaccaca gtctgatgtt ctcgatactt agacatacat tctagaaacc
     4321 gagacttcat ttccgtagct gtctcatatt gaataggact attaggaagc aactccccct
     4381 tgttttctaa aaacagtctt ctagctgttt caaagttttc tattcctgtt ttatagacct
     4441 gccattcatg taataaaggc tctactctta aaggaagacc cgtagcacag accacatacg
     4501 aagccgtttc taaagctctt gtgactgcag aagatacgat tatttcagct gacgagagta
     4561 aaggattttt gctcaatttc tggacttgct gccgtcccat ctcagacaag ggtgccaaat
     4621 ctatcccaaa tcctatataa gaacgctcct ctaactcacg gtaatctggc tccccatgac
     4681 gtacaaagat aatcttcatt ctagtgccct gtcgatccaa atccaccagt tcgaacgcca
     4741 tcagctgcat ctccatctgc aattaagaaa gtagcaaaaa cagcctggac aatacgttcc
     4801 ccaacttcaa gaacaacctc ttgatctgtg atattcttca tctgcgcaaa aatatgccct
     4861 tcatttccag gatttccata ataatcccca tcaatgaccc caactgagtt aattaaaacc
     4921 aagcccttct tacgaggatt tgaagaacga tcatagaggt agagaacctc agtcggctgc
     4981 atataagcct taacccctgt cggaaccaag acaatctctc ctggcgcaac aactgtacgc
     5041 acagcaacct ttaagtcgta accagccgca tgcgctgtct cacgcttggg caataaattt
     5101 tcatctgtaa aactcgaaac caattcaaaa ccacgaattt tcataatttt ctcttttcta
     5161 ttatcattta ttctagatta ttctatctta tttattcgga aaaagcacga aaaaaagagc
     5221 acacaacaat tataggcgat acgataattt acactgtttc acaatacgtt gaaatttaga
     5281 gctttaaagc gagggcaaag ttgatttttt acatcgtttt acagaggttt acgacacttt
     5341 tgcccctttt ttgccccttt gtttttcaaa aaaacttcat caaatcgctt gactttcacg
     5401 gtttaccgtg atataatata atcaagataa ggaaaggagg tgaggaagtt gaacaaagaa
     5461 gattggctta ggttactgga aaaggcgata gataatatcc ctgaaacggt aactgctata
     5521 gcaagtctgg tgactgcaat aacggtcgca aggcaaaaca aaaagcgtaa accgaaatcc
     5581 cgtcaaagaa aaaggtgaac gctaagaggt tggggcgaaa gcccctcaca cctctatttt
     5641 atcaaatgaa aagaggaaaa gcaatggtta gtgcaatagc tatttttata attgcggtca
     5701 atgtatatat ttatctaaaa aataaaaagg acaaataaga tgagagaagt tattcaagaa
     5761 ttgttagaca gttcgatgtc tacatctgct atttcccaag gtgctggagt tccatggact
     5821 actgtttctg acctcagaaa aggaaaaaca agcatggaca aaatggcact tctcacagca
     5881 gaaaaacttt atgaatttgc tacaactgat aagcagtgat ttcggtcact gtttttattt
     5941 ttgacaaaac accgtttttg acaataatca aaaaataaaa aggagctatt attaacaaaa
     6001 tggcgttttt ggcaataata ccctgccatt cctcctccct attcttcaag aaaacgcttt
     6061 tttgaagaat cagagcaaat aaaaaaccgc aagcctgagc ctgcggtgaa agaacaattt
     6121 agaaagtttc ctttctattt atttaactgt aatcaagcca tctggctcta ctgtgaactc
     6181 tggcttgtct gccagtgttc cgtctggttt gaggtagtac cagcctgttc cgtccgctga
     6241 ctggataaag gcatttgata ccatggcgcc ttctttagcg tctaagtagt accaagtgtc
     6301 cttgtacttg acccagcctg tcttcatggc accttctaca tcaaaatagt accacttgtc
     6361 agcgattttc ttccagcctg tggccatttc gcctgattgg tcaaagtagt accaattacc
     6421 gtctgtgtgc ttcttccagc ggtctgaaag catatagcct gagccatcga aataatacca
     6481 gataccgttg attttctcaa acttatagaa aggaggagat agtatggcaa atactccaat
     6541 aaaacctgga acagataatc agaaacctgg tcgctatgta gaggtaggac ctcgtggcgg
     6601 aaaagttact aatggtcata ccgcaactat cggaaaaggt gatcggctcc ctccgacatc
     6661 tgctaaaggc aacggttgga aaaaagtcta atcttcgttt gcgtacaatc gttcaatggt
     6721 tgtacgcttt ttccatacac aaaagcacat tcccaaaaaa tcaatttgaa tccatgcttc
     6781 ggcgtaatct ttcccattgc tggcatagtg agttatataa tggtgaatca ttttattcct
     6841 tcctcctatt gagttacttg agaagtatca tgaatggctt catagctaag acgcttaaat
     6901 tcttctgagt ctatctgaaa attgataggc tttttttgta aacactcaag aaaactagtg
     6961 tttcttaaaa gtttttcaac taactcaggg tctgccttta caaaggtgga ctctttttgt
     7021 ccactatacg gatatcgttt tggtctcatt ttcctactcc tcaaatcttt cctactcaat
     7081 cccataatct tcaataacct gaagaatgaa actgttcgct cgtggacctt tagtcgttcc
     7141 acttagaatg tttgtcactt cctgtcgttt aaagccgtaa gcaaccgcta gagttgcttt
     7201 tttaatgcct ttctctttca agaaagcaat aactctttcg cgaccgtttg cgatatctgg
     7261 catattttct cctttctttt tctttcttct ttttctgcta taatataagc agaaaggagt
     7321 taaccttatg acttttaaag aatatttact caaagcaagc aaacgcgaca tctacgatga
     7381 tggtaaagat tttgagaaat caatttcaga ttctcactaa tcgagcgtga cttttttgat
     7441 atggcaatct cagaaattta ccaccagcgc tgactacctt aatgactttt tcaaggtggt
     7501 ctttttcttg ttctaaattt ttaagaatac ttaccatcag cacgttcctc ctctttactt
     7561 atttgtaaat aagaaacaac taaaaatgta actatttttc tgcattatac ttgacaactt
     7621 acaccaaatc ggctaaaatt aaagcataat aaaaa
//
LOCUS       DAVMGZ010000093         7553 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000093 DAVMGZ010000000
VERSION     DAVMGZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.094"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            73..615
                     /locus_tag="VYQ93_001569"
     CDS             73..615
                     /locus_tag="VYQ93_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV3336232.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            615..1244
                     /gene="nth"
                     /locus_tag="VYQ93_001570"
     CDS             615..1244
                     /gene="nth"
                     /locus_tag="VYQ93_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV3336233.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            1455..1976
                     /gene="pyrR"
                     /locus_tag="VYQ93_001571"
     CDS             1455..1976
                     /gene="pyrR"
                     /locus_tag="VYQ93_001571"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV3336234.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            1995..2918
                     /locus_tag="VYQ93_001572"
     CDS             1995..2918
                     /locus_tag="VYQ93_001572"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV3336235.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            2968..4047
                     /gene="carA"
                     /locus_tag="VYQ93_001573"
     CDS             2968..4047
                     /gene="carA"
                     /locus_tag="VYQ93_001573"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV3336236.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMALDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            4359..7535
                     /gene="carB"
                     /locus_tag="VYQ93_001574"
     CDS             4359..7535
                     /gene="carB"
                     /locus_tag="VYQ93_001574"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV3336237.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         2195 a         1546 c         1719 g         2093 t
ORIGIN      
        1 tacaaagttt ggtgtgctga tttgtagcct tttcatggta taatcaagag agtaaatctt
       61 tatggaggaa gtatgaaatt aaatattcaa gaaattcgta agcagtctga aggtttgaac
      121 tttgaacaaa cgttagattt agttgatgac ctgcgtgcac gtaatcaaga aattttagat
      181 gtaaaagata tccttgcagt tgggaaagta caatatgaag accgtatgta tttcttagat
      241 tatcaactat cttataccat tgttcttgct tcgagtcgca gtatggagcc agttgagtta
      301 gttgaatctt atccagtcac ggaagttttc atggaaggcg caactaacca gctagatcaa
      361 gaagttttag atgatgactt ggtcttgccc atcgaaaatg gggcgcttga ccttgctgag
      421 agtgtatcag acaatatcct gctaaacatt cctatcaagg tcttgacggc tgaagaagaa
      481 gctggtcaag gatttgtctc aggaaatgac tggcaaatca tgacagagga agaataccaa
      541 gctcaacaag cagtaaagaa agaagaaaac agtccttttg ctggcttaca aggactattt
      601 gacggagatg aataatggtc ttgtcaaaaa aacgagcacg aaaggtgcta gaagaaatca
      661 ttgccctctt cccagatgcc aagcctagtc ttgattttac caatcatttt gaactcctgg
      721 ttgcggtcat gttatcagcc cagacgacag atgcagcggt aaataaggcc acaccaggtc
      781 tctttgttgc ctttccaaca ccccaagcta tgtctgtagc gacagagagt gagattgctt
      841 cacatatttc tcgcttggga ttgtatcgga ataaagctaa attccttaaa aaatgtgccc
      901 aacagttact agacgatttt gatggtcaag tccctcagac acgtgaagaa ttggagagtt
      961 tggcaggtgt tggtcgcaag acagccaatg ttgtcatgag tgtaggattt gggattccag
     1021 cctttgcagt ggatactcat gtggagcgta tttgcaaaca ccacgatatt gtcaaaaaat
     1081 cagcgacgcc acttgaggtg gaaaagcggg tcatggatat cttgccgcct gagcagtggt
     1141 tagctgccca tcaggccatg atttattttg gaagagccat ttgccatcca aaaaatccag
     1201 agtgtgacca gtacccacaa ttatatgatt ttagcaattt gtaagatgct acattgaaac
     1261 tttttgcttg attttaagca tgctttgtgc tatagtaaat gataactaaa tattccttta
     1321 aacctgtccc gtgaggcagg caaggagcgc gataaagtag aagggtgaag tctacactgt
     1381 gtgcagtagg gctcgcttgg ctataaattt caagtctcct tgtttaagga gacttttctt
     1441 tttggaggtt tgtcatgaaa acaaaagaag ttgtagacga attgaccgtc aaacgagcga
     1501 ttacgcgtat tacttatgag attatcgaac gcaacaaaga tttgaataag atcgtcttgg
     1561 ctggtattaa aactcgtggt gtctttattg cccaccgaat ccaagaacgt ttgaagcagt
     1621 tagaaaatct ttcagttcct gttgtggaat tggatactaa acctttccgt gatgatgtta
     1681 aaagtggaga agatacttct ttggtttctg tcgatgtgac agaccgcgaa gttatcttgg
     1741 tggatgatgt gctctataca ggtcgtacca tccgtgctgc tattgataat attgtcggtc
     1801 atggtcgtcc tgcgcgtgtg agtttagcag ttctagtcga tcgtggacat agagaattgc
     1861 caatccgtcc agattacgtt ggaaaaaata tcccaaccag tcgttctgaa gaaatcatcg
     1921 tagagatgac agaacttgat gaccaagaca gagttctgat tactgaagaa gcttagaaag
     1981 ttaaaggagt agccatgtca gaaaatcaac aagcattgaa ccatgtggtg tccatggaag
     2041 acctcactgt cgatcaagta atgaaattga tcaagcgagg aattgagttt aaaaatggag
     2101 cccagcttcc ctatgaagac catccgattg tttccaatct cttctttgag gattctacac
     2161 ggacacataa gtcctttgaa gtcgcagaga ttaaacttgg attggaacga cttgactttg
     2221 atgtgaagac tagttcggtt aataagggtg agacacttta tgacaccatt ttgactctgt
     2281 ctgctttagg agtggatgtc tgtgtgattc gccatccaga ggtcgactac tatagagaat
     2341 tgattgctag tccaacgatt acgacttcca tcatcaatgg tggagatggt tcgggccaac
     2401 accctagcca gagcttgctt gatttgatga ccatttatga ggaatttggc cactttgagg
     2461 gtcttaaagt tgctattgca ggtgacttgg atcactcacg cgttgccaaa tccaatatgc
     2521 agattttgaa acgcttggga gctgaactct ttttcgctgg acctgaggaa tggagaagtc
     2581 aagagtttgc agactatgga cagtttgtaa ccattgatga aatcattgat caggttgatg
     2641 tcatgatgtt tctccgtgtg caacacgaac gccatgatag tggagctgta ttttcaaaag
     2701 aagactacca tgcccaacat ggcttgactc aagaacgtta cgatcgtttg aaagaaacag
     2761 caatcctcat gcacccagct ccaatcaatc gtgatgtaga aatagcagac cacttggttg
     2821 aagcaccaaa atcacggatt gtccaacaaa tgaccaatgg tgtctttgtt cgaatggcaa
     2881 tcttagaatc cgtactagcg agtagaaacg ccaactaaaa cacaagacgt taaaagacgc
     2941 cagtgagcgt ccacgagagg tgaaaatatg acaaaaagaa ttctagtatt agaagatggc
     3001 acagtttttg aaggcaaggc cttcggagca gatattgatg taacaggcga aatcgtcttt
     3061 aatacaggga tgaccggcta ccaagaatcc attacagacc agtcttataa tggacaaatc
     3121 ttgaccttta cttatccttt ggtaggaaat tatggtatta atcgtgatga ttatgaatcc
     3181 attattccaa cttgtaaggg agtcgttgtt tttgaagaag cgcgtagagc tagcaactgg
     3241 cgcaaccaaa tggccttgga tgaatttttg aaagccaaga aaattccagg gatttcagga
     3301 attgatacgc gtgctcttac caagattatc cgtaagcatg gtactatgcg tgcaaccttg
     3361 acccatgttg gggacagtat ggaccatgtg acggaccagc tccaagcaac agttttaccg
     3421 acagacaata tcaaacaggt ttctactaaa acgtcttatc cagctccagg agttggtttg
     3481 agcgtggtgc tagtggactt tggtctcaag cactcaatcc tacgtgaact ttctaaacgc
     3541 aactgtaacg tgacagttgt tccttattcg acaacggctg aagaaattct ccatctcaat
     3601 cctgatggag ttatgttgtc aaatggtcca ggtaacccag aagacgttcc acaagcactc
     3661 gacatgattc gcggtgtaca aggaaaaatt ccaatctttg gtatttgtat gggacaccaa
     3721 ctctttgcaa tggcaaacgg ggctaagacc tataagatga agtttggcca tcgtggattc
     3781 aaccatgcgg tacgtgaaat cgcaacagga cgagtagact tcaccagcca gaaccatggt
     3841 tatgcagtca gccgcgagga tttgccagag cacttgatta ttacccacga agaaatcaat
     3901 gacaagtcag tggaaggtgt gcgccacaga taccaacctg ctttctctgt tcaataccac
     3961 ccagatgcag ctcctgggcc acacgacgct agctacctat ttgacgagtt tatcgagatg
     4021 atggaagttt ttaaacaatc aaactaagaa cgagtaaaag ctcgtcggag aatttatgta
     4081 actgaacacg gacggaaagc tcagaattta gagaaaccaa cttggattgt caatccttcc
     4141 ttgatttctt aaaattcaat cgctttctat tcgtcctttg tatcttattt aatgtcgccc
     4201 tgagaggcga cgaatagaaa ggagaagata cattgttcgc ggaagtgaac acgccctaag
     4261 aactgtgtga aaaagataaa catcgtcttg acgctgaagg cgtctgcgcc gagtttccta
     4321 ttttcactgt gttctttacg ggctttgtat cataaactat gcctaaacgt actgatattc
     4381 aaaaaattat ggtgattggt tctggtccga ttattattgg tcaggctgct gagtttgact
     4441 acgctgggac ccaggcttgc ttgtcgttga aagaggaagg ttatgaggtt gttttggtta
     4501 actcaaatcc tgcaaccatc atgacggaca aggaaattgc tgacaaggtt tacattgaac
     4561 cgattacact tgagtttgtg acacgtattc ttcgtaagga acgtccagat gccttgctac
     4621 caacactcgg tggtcagaca ggtctcaaca tggccatgga attatctaaa aatggtatcc
     4681 tagacgagct tggcgttgaa cttctgggta ccaaattatc tgccattgac caagcggagg
     4741 accgtgacct ctttaaacaa ttgatggaag agcttgagca acccattcca gaatctgaaa
     4801 ttgttaacac agtagaagaa gcagttgcct ttgcagcgac aattggctac ccagtcatcg
     4861 ttcgtccagc ctttaccctt ggtggtactg gtggtggtat gtgtgccaac gaaaaagaat
     4921 tgcgtgaaat cactgaaaat ggtttgaaat tgtcacctgt tacccaatgt ttgattgagc
     4981 gttcgattgc cggttttaag gaaattgaat acgaagtgat gcgtgactca gctgacaatg
     5041 ccttggttgt ttgtaacatg gaaaactttg acccagttgg gattcacaca ggggattcca
     5101 ttgtatttgc ccctgcgcaa accatgtcag actatgaaaa ccaaatgctt cgtgatgcga
     5161 gcttgagcat tattcgcgcc ctcaagattg aaggtggatg taatgttcag ctagctcttg
     5221 atcctaacag cttcaagtat tatgttatcg aagtaaaccc tcgtgtatca cgttcgtcag
     5281 cccttgcttc taaagcgaca ggatatccga ttgctaagtt ggctgccaag attgcagtag
     5341 gtttgacctt ggatgaggtt ataaacccag ttacaggttc aacctatgcc atgtttgaac
     5401 cagcccttga ctacgtagtt gctaagattc cacgtttccc atttgacaag tttgaaaagg
     5461 gtgaacgccg tctcggtaca caaatgaagg cgactggaga agtcatggca atcggtcgta
     5521 acatcgagga atctcttctc aaggcctgcc gctcccttga aattggggtg caccacaatg
     5581 aaatacctga acttgcagca gtttctgatg atgccttgat tgaaaaggtt gtcaaagccc
     5641 aagatgaccg tctcttctac gtatcagaag ccattcgtcg tggctacaca cctgaagaaa
     5701 ttgcagagct tacaaaaatt gatatcttct atcttgataa actcttgcat atctttgaaa
     5761 ttgagcaaga attgggtgct catccacaag atctagaagt tttgaaaaca gccaaactta
     5821 atggtttctc agaccgtaag attgctgaac tctggggaac gactgatgat caagttcgcc
     5881 aacttcgctt ggaaaacaag attgttccag tttataagat ggtagatact tgtgcggcag
     5941 agttcgactc tgaaacgcca tatttctatt caacctatgg ttgggaaaat gaatctatca
     6001 gatctgataa agaatctgta cttgtcctag gttcaggtcc aatccgtatc ggtcaagggg
     6061 ttgagtttga ctacgcaacc gttcactctg ttaaggctat ccaggcagct ggttatgaag
     6121 ctatcatcat gaactcaaac ccagagaccg tttcaacaga cttctctgta tcagataagc
     6181 tttactttga gccattgaca ttcgaagatg ttatgaacgt cattgacttg gaacaaccaa
     6241 agggagttat cgttcagttt ggtggtcaaa cagccatcaa ccttgcggag ccattggcaa
     6301 aagcaggtgt gaccatcctt ggtacacagg ttgctgacct agaccgtgcc gaagaccgtg
     6361 acctcttcga gcaagctctc aaagaattgg atattccaca gccaccagga caaacggcta
     6421 ccaatgaaga agaagcagct cttgcagctc gtaagattgg cttcccagtt cttgtccgcc
     6481 catcttatgt acttggtgga cgtgccatgg aaatcgttga aaacgaagaa gaccttcgtt
     6541 cttacatgcg aactgctgtt aaggctagtc cagatcaccc agttcttgtc gactcttata
     6601 tcgttgggca agagtgcgaa gttgatgcca tttcagatgg taaagatgtg ctcattcctg
     6661 gtatcatgga gcatatcgaa cgtgctggtg tccactcagg tgactcaatg gctgtttacc
     6721 caccacaaac cttgtcgcaa aaggttcagg agaccatcgc agactacacc aaacgcttag
     6781 caatcggtct tcactgcctt ggaatgatga acatccagtt tgttatcaag gatgaaaaag
     6841 tctacgttat tgaggtcaat ccacgtgcca gccgtactgt tccattccta tctaaggtaa
     6901 ccaatattcc tatggctcaa gtagcgacca agctcattct tggtcaaagt ttatcagaac
     6961 ttggctacca aaatggactt taccctgaaa gcacccgtgt tcatatcaag gcacctgtct
     7021 tctcctttac caaactagct aaggtagaca gcttgctcgg tcctgaaatg aagtcaacag
     7081 gtgaagttat gggttctgat gctactttgg aaaaagctct ctataaagcc tttgaagctt
     7141 cttatctaca cttgccaact tttggtaatg tcgtctttac catcgctgat gatgccaaag
     7201 aagaagcctt gaacttggct cgtcgtttcc aaaacattgg ttatggaatc cttgcgacag
     7261 aagggacagc agccttcttt gctagtcatg gcttgcaagc ccaacctgtt ggtaagattg
     7321 gtgatgacga taaggatatc ccaagttttg tacgcaaagg aagaattcaa gctatcatta
     7381 acacagtagg aacaaaacga actgctgacg aagatggtga gcaaattcgt cgttcagcca
     7441 ttgaacacgg ggtgcccctc ttcacagcgc tagatacagc taatgccatg cttaaggtac
     7501 ttgaaagtcg tagctttgtt acagaagcga tttaaataga aaaataagat ata
//
LOCUS       DAVMGZ010000094         7274 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000094 DAVMGZ010000000
VERSION     DAVMGZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.095"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            451..676
                     /locus_tag="VYQ93_001575"
                     /pseudo
     CDS             451..676
                     /locus_tag="VYQ93_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(1410..2915)
                     /gene="pyk"
                     /locus_tag="VYQ93_001576"
     CDS             complement(1410..2915)
                     /gene="pyk"
                     /locus_tag="VYQ93_001576"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV3336238.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDEVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(2974..3981)
                     /gene="pfkA"
                     /locus_tag="VYQ93_001577"
     CDS             complement(2974..3981)
                     /gene="pfkA"
                     /locus_tag="VYQ93_001577"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV3336239.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            complement(4064..7165)
                     /locus_tag="VYQ93_001578"
     CDS             complement(4064..7165)
                     /locus_tag="VYQ93_001578"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV3336240.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPFYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
BASE COUNT         2218 a         1598 c         1277 g         2181 t
ORIGIN      
        1 aaacaatata gactagtaat ggttggactc aataagaatt gggtactaag ataggtataa
       61 gtaaaaacac cattagaaat tatgaaaaaa gggttaggtc tactaagaaa aatactatat
      121 ttgacttagt taaagtattc agtagcttga ttgatgccct ctttcctcca gttcaaaagg
      181 attctcctag cgatatccaa tctatctacg accaacgggc accacccaga caaggtaaag
      241 ttctgaccta tgccgagagg caactgtatg atcagaaaaa cgaagtatcg taaaaatcat
      301 cagactggac gactacagac agactactta ctctcgtgtt aggtagttta tctctgcagg
      361 tagccgcttg atgcaggacg acgatttagt tatggagttt ccattatccc agacgactac
      421 gacccaatcg cttatgtcgt cagcaagtcc atggagccaa agataagaaa cagcgactac
      481 ctatttatca agaatacccc acagattgac tataatacca tcggcatctt ccaagtagac
      541 ggcactaact atgtcaagaa actccgccaa ggctacctca aaaaaactca atcccgaata
      601 ctccgacatc cacctagacg aaagcaacca tattcgtacc atcggaaaag ttgtcaggct
      661 atatagggag aattaaaaaa gggggagaat ggcgatataa aacaaacaga ttttaaaaga
      721 ttcgaaaaac tttgaaaaag ttcattctac atttcaaaaa aagaataagt gacctaaaat
      781 agattagatt ttgttaaaca atagtgatat gtaaatttag agaaaggcat ttaagatcac
      841 tttgcaaaag gcaaatacta aactagagaa accgataaac cagcccctta gttcccatat
      901 tttcaggcac acactcctta gtacactagc tgagaagaat gctataatgg ttagagtaga
      961 acacaaaaac gctaaaacag taaataacat ctagactcac gtaggtaaaa gaatggagca
     1021 agctactttg gaggttttaa ataccatatc attaaatcga aaatatatca gatcgaatct
     1081 agacaagtag attgcgattg ctaaaacatt tgtagaaatt catttgactt tctaatcaat
     1141 ttatgcatat cttatttcat actcaatgaa aatcaaagag caaactagga agctagccgc
     1201 aggttgctca aagcactgct ttgaggttgt agatagaact gacgaagtca gtaacatata
     1261 tacggtaagg cgacgctgac gtggtttgaa gagattttcg aagagtatca atccattatg
     1321 aaagttttgc cccctttttg ccccctggac aaaaaaagag cccatcatac aggctagaaa
     1381 gctggatatg ataggttttt atatttttct taacgtactg tgcggatacg cattgtgttt
     1441 gtgcgaacag cttctcctac tggcacacca gcaacgataa cgatatcatc gcctgactca
     1501 acgagacctg cttctaccgc tttacgttca gcgatttcga acatatcgtc agttgaagat
     1561 ggagcatctg tcaacattgg gataacaccc cagttcaaca tcaagccacg ttctgtcaat
     1621 tcgtcaaatg tcaatgctaa gatgtcagca tttggacggt atttagaaat caaacgtgca
     1681 gtatgacctg tcttagtaag agttacaacc aatttgatat ccattgagct agtagcatct
     1741 ttaacagcag aagccattac ttctgtctta gagttacgct caaatgaatc tgaatcaaga
     1801 cgtccgtatt cattaagaag agcttgagcg ttcttgtcga ttgtagccat tgtagttact
     1861 gactcgagtg ggtatttacc gtttgcagac tcgcctgaca acattgtagc gtcagttccg
     1921 tcgataacag cgttgaatac gtctgatact tctgaacgag ttgcacgtgg tttttcagtc
     1981 attgtttcaa gcatgtttgt tgcagtgata acaactttac ctgcagcatt gactttcttg
     2041 ataatcattt tttgataaac tggaaccatt tcgaacggta cttcgatacc catatcacca
     2101 cgagcaatca taataccatc agctgcttcg atgatttcat ctaagttatc gataccttgt
     2161 tggttttcga ttttagcgaa caattgaaca tgtccgtttc cagtttcttc acagattgca
     2221 cgaacttcgt tcacatcttt tgcagtacgt acgaatgaaa ttgcgatgaa gttgatacct
     2281 tgttcaagac cgaaacggat atcgtcgtta tcgcgttcag caagagctgg gaaaggaatt
     2341 ttagtgttag ggatgttcac acctttttgt ttagcgatga taccatcgtt ttcaacttca
     2401 acttcaaatt cacgagttgc atcatcttta gcaaccacac gaagaccaag tttaccatcg
     2461 tcaaccaaaa cttgacgacc aacttcaact tcatcataga tatcaagagc accagcaacg
     2521 ttcaacgcaa tcacttcacg agttgatttg attccttgtt tagttgcaac acgaattttt
     2581 tcaccagttt tgtatgaata ttctttagct tcaccttcga acaattctgt acggatttca
     2641 ggtccttttg tatcaagaag gaaaccaact tttttacctg caattttttc cgcaagttta
     2701 acagttgcca tacgctcacc ttgttcttgg tggtcgccgt gtgagaagtt gaatcggaat
     2761 gtgttagcac cagcttcaat caatttagca atgtttttag ctgaagcttc aacatcaagt
     2821 ttttcacccc agtatccgtc ctcaccgaat tttttaccac cacggatttc taccgcagga
     2881 cccaaagttg caacgatctt tacacgtttg ttcatgattt ttgtgactcc tttatatata
     2941 tgaccttttt ggtcttatta actaaattgt aaattatgac aagctcttat tcaagctaga
     3001 tagctcaata tcggctttgt gcgggttgtt aaccacaatc ttaccttctg cagtaaggct
     3061 aaacaatgcc ccttcttctg cagtaccaag aattggattt tcaaccattt tttcgttacg
     3121 aataccaacc gcaacaccac cgataccttc tttaagaagt ttaacagcat gtgcacccat
     3181 acgtgacgcc aaaacacggt cacgcgcagt tggagaacca ccacgttgaa tatgtccaag
     3241 ttctgttaca cgaaggtcgc ttgtatctcc agcttcttta agtttttgac caaattcagc
     3301 cgctgacatc acaccttcag ctaagacgat gatattgtgt tttttaccac attcataacc
     3361 agctttgatg cttgctacga tatcttccat cttgaagcct gcttcaggga tgatgatttc
     3421 atcagcacca gttgcaatac cagcccaaag agcgatatca ccagcgttac gtcccataac
     3481 ttcaattaca aaagtacgac ggtgacttga tgatgtatca cgaatcttat cgatagcgtc
     3541 catggcagta gtaaccgctg tgtcaaaacc gattgtaaag tcagtaccaa cgatatcgtt
     3601 atcgattgta cctggaagac caatagctgg gaagccatgt tcagtcaaac gcatagcgcc
     3661 gtggtaagat ccgtcaccac cgataacaac tacaccttca attccgtgtt ttttcaattg
     3721 ctcaatccct ttaagttgcc cttcaagttg agcgaactct ggataacgag ctgagtgaag
     3781 aaaagtacca ccacgagaaa tgatgtcccc tactgaagct gcatctaggg gatgaatttc
     3841 accggcaacc ataccagcat atccgtcata gataccaaac acttccattc cttctgaaat
     3901 tgcttgacga acaactgcac ggatggcagc gttcatacca ggtgcgtctc cgccactagt
     3961 caaaacagca atacgtttca tattggttta tgctcctttt tcttttaaca ttcttcttga
     4021 ttatatcaca tttgatttta aaattcttct attttccgta tttttagcga taaatcgttt
     4081 tcataacgat ttcattcaat ttctcctcta attcattgga tttagctaca aaatgatggg
     4141 gagaaacgat ggttttctgt tcctcttcat accggatgat gactgggatt gggcctttaa
     4201 attgttctaa aatgcgtgaa atttcttgat ccgattcatg atttttcacc tgtatccaaa
     4261 agcgttcagc aactgcttct cttatttctt gtgcaatcat ttgcagacgg ccatcacgtg
     4321 attgtatttt tccttttaca tagtagaagg ctccctcttt tatttcctgt ccaacctgac
     4381 gatataagtc tgaaaagaga gtgacatcca attttttctt actatcatct gcctgtaaga
     4441 aggccatatt ttcacccttt ttggtacgaa tcacttttat tttctgaact tcaaccaaga
     4501 taatagcata gctattttct gacaaatttc cgattggggt aatcgggtaa atagccttac
     4561 ttgcaatagc ttgtagtgga tgtttgctga cacctatccc taaaagctct tgttccatat
     4621 aaaatttttc ttgttccgtc caatcttccg attcctgcca actataaata gcatctccaa
     4681 acaaacttcc caactctttc acaaattcaa atagattagc taaattatta aatacttttt
     4741 gacgattttt ttcaaatgaa tcgaaaagac caacttttac caaaggttct agcagaggaa
     4801 gtttcagata attctcaggt aatttagcta taaaatcttc aatgttagaa taaggtctat
     4861 tttcaataat ccagagagct aaatcattac tgactccttt aatggatttc aaacctagat
     4921 agatggcctt gttggcaatt ttatcgtgat agggaatggt gttgatggat agagaggcta
     4981 cttcaaaacc tgcttcaagt gcatctatta agtaatcact gttggaagaa tttaacatga
     5041 cctgataaaa aatggctgga taatgtgttt taaaataagc caactggaag gccaaggctg
     5101 agtaggcata ggcgtgtgac ctgttaaaac cataacctgc aaacttctcc ataacatcaa
     5161 agacctgctc tgctttttcc acagtatgac cagcttctaa tgaaccttga ataaaggaag
     5221 ccctcatctc atgcatggca gaggcatcct ttttccccat agcccgacgc aaaatatcgg
     5281 ctttcccaag actaaatccg gcaaatcgct gggcaacctg cataacctgc tcctgataga
     5341 gcattatgcc gtaggttgga gccaaaatat cctccagtac tggatccaga acagtcactt
     5401 cttcctgccc atgctttctt gccacaaaat tattgatata gtcactagca cccggtcgat
     5461 ttagagaagt agtcgcgacg acatcttcaa aacagactgg ttgcacacgc ttaagcagac
     5521 gaatggcacc tggttgctca aattgaaaga taccttttgt atttccagag gcaaataaag
     5581 ctaacgtttc tttgtcttcc aaatctattt cttcaatttt aaggtgaata ccttctattt
     5641 cagcaagcaa ctcttgcatc ttctggacaa aggtcaaatt tcgtagtccc agaaagtcca
     5701 tcttcaaaag tccgctagcc tcaactccat gagcatcata ctgagtcagt ggaatttcat
     5761 caccatactt tagaggaata tagttggtta aatcttggtc actaattaca acaccagccg
     5821 catggacaga ggtttgtctt ggatagccct ctatcttgca agcaatctca aaagcttttt
     5881 ggtattctaa cttactattg atttgctgac gaaactggag attgccctca taggccgact
     5941 taagattgtc acgaaaactg attttcttag taattgcaga taattcatac tctggcacac
     6001 caaagcgttt caagacatct cgaagagctt gcttggctcc aaaggttgaa aaagtaacga
     6061 tttgtgccgc atgtttacta ccatatttat taccaacata tctgataaaa tctggacgat
     6121 aaatatctgg gatatcaata tcaatatcag gcatggtata gcgttcacga ttaagaaagc
     6181 gttcaaaaat cagatttttc tctactgggt caatccccgt gatgtctaag gcataagaaa
     6241 ccaaactgcc tactgcagaa ccccttccca ttcccatata atagccattc gatcgtccaa
     6301 aacgcaacaa atcccaaaca accaagaaat aatcatcaaa gcccatatca tgaataacag
     6361 acaattcttg gtctagtcta tcttgatatt ctttactagt caaccccttc tgaacaagcc
     6421 ccagttcagc acgctctctc aactcctcta ctgctggtct agctggatta aaacgaggca
     6481 gtttcagact agtatccaag tcgtaagaaa tgcctgaaat aagcttttct aaattgtcca
     6541 aagcttgcgg aaaacactct tggaatagtt tctctaaaga acttgctgat ataaagacat
     6601 cttgtctcga acgcaaggga acttctctga gcggtagatt ttctttaatc gctgttaaaa
     6661 cttgaagaac ttctctatcc ctgctttcaa aagcgttgac ccgataaaaa ggtaagatag
     6721 gatgatgaaa ttcgcttgct agtgtttctg gataaacccc tatatagtaa tcacagccta
     6781 gttctaacga ctcaactcta tcaaaataag gcacaatgac cgcgatatcc tccaggtact
     6841 gggacaggac tgaccaagtt ttctccccct gcatcttggc tgtcgaaagc ttcatcaact
     6901 gctgatagcc cacactagat agagctaaaa agcgcaaatt cactccctga tcatctacaa
     6961 acactgtcat ttcaagccct agcaaaggat gaatgccgta tttttttgta atctctagaa
     7021 agtcgaaagc gccataaaga ttgtcaatat ccatcatagc caaatgagtg tagccgtatt
     7081 ctttagctgc tctcacatac ttttcgatcg aaatgacgct ttccataaaa ctatagactg
     7141 tttttgtatc tagttgtgcg atcaatttac acttctcctc tatccttctc actatattat
     7201 accattttca cctataaatg gcttctcttg agaaaaattt catcagctta ctaatttatt
     7261 ttctattcat ttgt
//
LOCUS       DAVMGZ010000095         7210 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000095 DAVMGZ010000000
VERSION     DAVMGZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.096"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(66..143)
                     /locus_tag="VYQ93_001579"
                     /pseudo
     CDS             complement(66..143)
                     /locus_tag="VYQ93_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(193..2190)
                     /gene="metG"
                     /locus_tag="VYQ93_001580"
     CDS             complement(193..2190)
                     /gene="metG"
                     /locus_tag="VYQ93_001580"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV3336241.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPEGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDCQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2314..3573)
                     /locus_tag="VYQ93_001581"
     CDS             complement(2314..3573)
                     /locus_tag="VYQ93_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3336242.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESL
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3575..4276)
                     /locus_tag="VYQ93_001582"
     CDS             complement(3575..4276)
                     /locus_tag="VYQ93_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336243.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4260..5459)
                     /locus_tag="VYQ93_001583"
     CDS             complement(4260..5459)
                     /locus_tag="VYQ93_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV3336244.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PIHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5724..7070
                     /gene="gor"
                     /locus_tag="VYQ93_001584"
     CDS             5724..7070
                     /gene="gor"
                     /locus_tag="VYQ93_001584"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV3336245.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAASEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR"
BASE COUNT         2096 a         1603 c         1358 g         2153 t
ORIGIN      
        1 cagtgctttg agctgacttc gtcagtttca tctacaacct caaaaacatg ttttgagctg
       61 acttcgtcag ttctatctac aacctcaaag cagtgctttg agcaacccgc ggctaacttc
      121 ctagtttgct ctttgatttt cattgagtat aaataaaaaa acagaactag tctgaactag
      181 tcctgtctac ttttacccaa tcacacttcc atttggtaca gctggatcaa ctgtgagaag
      241 ggttaatttg ccatcatgtt cagctgagag aatcataccc tggctgacat attttttcat
      301 cattttacgt ggtttgaggt tagcaacgat ttgaactttc ttgccgacca attcttgttc
      361 atttggatag tattttgcaa ttcctgaaag aatctgacaa tcttctccat caccagcatc
      421 caagcggaat tgaagcaact tatctgaacc ttctacttta gacacttctt tgacttctgc
      481 gacacggatt tcaaccttgt caaagtcttc aaacttgatt tcatccttgt ttagtttgag
      541 ctcaacttcg tccggattcc attctttttc gactgctggt ttattgcctt ccatttgttc
      601 cttgatatag gcgatttctt cttccatatt tagacgtgga aagataggtg ttcctttggc
      661 aactacagtc acatctgctg ggaagtcagc caaactcaag ttttcaagac tagaaacttc
      721 ttccaaacca agttgagtca aaactgcacg actagtttcc atcataaatg gttcaatcaa
      781 gtgagcaact acacgaatgc tggctgccaa gtggctcatg acacttgcca attggtcacg
      841 aagagcttca tccttgtcca agacccatgg tgcggtctca tcgatgtatt tattggtacg
      901 agagatcaga gtccagactg cttcaagcgc acgtggatag tcaactgctt ccatgtgtgt
      961 atggaagtct gcgattgatt gttctgcaac ctcagcaaga acatgatcaa attcagtcac
     1021 accttctaca taggcaggga tttgtccatc aaagtactta ttaatcatgg aaaccgtacg
     1081 gttaaggagg ttcccaaggt cattagccaa ttcatagttg atacggccga catagtcttc
     1141 aggagtaaag gttccgtctg aaccaactgg aaggttacgc atgaggtagt aacgaagtgg
     1201 atctagtcca taacgctcta ccaacatttc agggtaaacg acattccctt ttgacttaga
     1261 catttttccg tctttcatga caaaccaacc atgggcaatc aaacgatcag gtaatttaac
     1321 atccaacatc ataagaagga ttggccagta gatagagtgg aagcgaagga tatcttttcc
     1381 taccatatgg aagactgttc cattccagaa cttgtcaaag ttaccatgtt cgtcttgagc
     1441 gtagccaaga gctgtcgcat agttaagaag ggcatcaatc caaacgtaga caacgtgttt
     1501 tggatttgat gggacaggca ctccccatgt aaaggttgta cgagataccg ccaaatcttc
     1561 caaacctggc tcgatgaagt tgcgtagcat ttcattaagg cgaccctctg gcgtgataaa
     1621 ttcaggatga gctttgaaaa attcgaccaa acggtcttgg tatttgctaa ggcgaaggaa
     1681 gtatgattct tcagaaaccc attcaacctc atgacctgat ggagcaatac caccagtcac
     1741 atttccagct tcatcacgga aaacttctgc cagctggctt tctgtaaaga attcttcgtc
     1801 tgatactgaa taccaaccag agtattcacc caagtagata tcatcttgag caagtaagcg
     1861 ttcaaagact tgtgcgacaa ctttttcatg gtagtcatca gttgtacgga taaatttatc
     1921 gtatgagata tctagtaatt gccagagttc tttaactcca accgccattc catcaacata
     1981 ggcttgaggt gtaataccag cttcttccgc tttctgctgg attttctgac catgttcatc
     2041 aagacctgtc agataaaata catcgtagcc catcaggcgt ttgtaacgtg ctaggacatc
     2101 acatgcgata gttgtgtagg cagaaccgat atgaagtttc ccagatggat agtaaatcgg
     2161 cgttgtaata taaaaatttt tttcagacat aatttttcct ttccaggcaa atgaaacctg
     2221 tttttctaac acttcattat atcacatttt taatgaattt cgatagggaa atccatacaa
     2281 aaacaagata gacgagtgtc catcttgttg atctcattca taacgaaggg cttcaattgg
     2341 atcaagtttc gatgccttgt tggctggcaa gactccaaaa atcataccaa cactagccga
     2401 aactgcaaga ctaaataggg caactgggat tgatactcca acttctatac cttctattaa
     2461 accttgcagt aacaaacctg ctaaggcagt taaaccactt gcaattgtca agccaattaa
     2521 gccacctaac aaggtcaaaa tcatggattc aatcaaaaac tgaattaaaa tattggcacg
     2581 tgttgcaccc aaagccttac gaagaccaat ctcacgagtg cgctctgtca ccgaaaccag
     2641 catgatgttc atgacaccag ttcctccaac aaagagagaa atccctgcga tggaactaat
     2701 aatcgtcgtc ataaaactaa acgattgttg aatttctgca aatacaaggg actcatctgc
     2761 cacctggtat tctccctgtt gtaagcctgc aagctctgtc atttttcgtg ccagttctgg
     2821 acccagagtt ggggttaaac tggtatcatt cactcgaaag acaatattag ctatttcatc
     2881 tacattaaaa ttcgcagcaa gggagatatt ggtagtaata ggcaagccac caaacccata
     2941 tatttttgat cttttagcct ccggactagt ataaacccca atgacccggt aactaaatcc
     3001 attgacttct acaaccttgt taatagcctc ttgaggagat tcaaataaac taatggacaa
     3061 ttcctcatct agcaaaatga cacttgcaaa ctctttgaaa tcttgctctc tcagactacg
     3121 acctgcaata atttcattct taacagcgtc catgtaagtt ctgtttccac ctgtcaaatt
     3181 agcattctca acctttttat cttgataggt caagatggca ttcgttgaat tggttacata
     3241 gtaactatcc actcccttca gtttagctgc ctcttggacc caggattctt gcggttttgg
     3301 cggttcaaca ggaacttcct cttcctttcc agaaaccgta aaagctgatt gtttctgagt
     3361 aaaagacccg tctttacttt ttttaggaga gaaaaagacg ctaatatttt tctgagattt
     3421 agtcatatct ttattgactt gacgagatag ggaatcaccc aaagccataa tcacaacaac
     3481 tgatgaaaca ccgataataa tcccaatcat agtaagcaaa gaacgcatct tgtgagccat
     3541 gatagatgaa aaggcaaatt tcagattctg catcttagtt ttcctccttt cctaactgag
     3601 cactgtcaga cgaaatgacc ccatcccgaa tgacaatctg acgtttggca taggcagcaa
     3661 tctcaggctc atgcgttacc atgataatgg tttttccttc tttattcaaa tcaaccaata
     3721 attgcataat ttggttacct gttttggtat ccaaggctcc tgtcggttca tccgctagga
     3781 taatagaagg attgtttacc aaggcacgcg caatggctac acgttgcttt tgaccaccag
     3841 ataattctga aggtaaatgg tgactacgtt ctgtcaattc aaccttgtct aaatattcct
     3901 cagccaactt gcgacgtttt gaagacgaaa ctcctgcgta aatcaagggc aattctacat
     3961 tttgcagagc attgagcttc gatagaagaa agaactgctg aaagacaaaa ccgatttgtt
     4021 ggttacggac cttagctagt tgtttttcac caagcccagc cacttcttga ccttcaagat
     4081 aatattctcc actggttggt gtatccaaca tgccaatcgt attcatcaga gtggacttac
     4141 cagacccaga tggtcccatg atggctacaa attcaccctc attcacttct agattgatat
     4201 ttttgagaac ctgcagttct tggtcaccat tacggtaact tctgaagata ttttttagac
     4261 taattagttg cttcatcagc cttcacctct tttccttctt ccaaggaaga tgttggatta
     4321 ctgatgacct tagcaccgtt cgttaaacca gaagtgattt cttgattttc tgcgtcagca
     4381 tttcccaatg aaacctcaac ttttttagcc ttttgttgtt catccacaat ccagacataa
     4441 tttttactat catccattac tagactgcta acaggaacaa gaatagcctt agttttgctt
     4501 ttaacctcaa tgttgacaga aaaaccctgt ttcaaatcac caacctcgcc tgtcacatca
     4561 atagtataag ggtatttaga acctgtatta ttcccggctg ctggactagc tgcttcacca
     4621 ttgtttttag gatagtcaga aatatagctt aatttcccag tccatttttt atcaggatac
     4681 actttagaag taaagcttac ttcttgacct acagaaaggt tggctagatt gtactcagat
     4741 aattctccct tgacttgtaa attttcattg ctgacaatat gaaccataac ttggctcgcc
     4801 cctgttggag atttagaaac attgctattg acttcgacta cagttccctc tagggtactg
     4861 agaacagttg ttgcatccaa ttgactttga gccttgctta attgcgccgc agcatctgca
     4921 cgcgcatcac gggcatcacc caattgagcg tcaatagaag caacagaatt tccagccact
     4981 ggagttgggc tttgcaccgt tgcatcttct cctcctactg gcgctggtaa ctgtggagcc
     5041 ggagctgaag cggcttcatt tcgtgcttga ttgagttcat tgatatgacg atctgcccta
     5101 gctactgctc gactagctga atcataggcc gcctgcgctt ctgaactact gtacttgact
     5161 aaagcctgcc cttcgctgac cttatcgccc acagaaacaa ggatttcatc taaatcaccc
     5221 ttactagcat caaaataaac atattgttca ttttttgctg ttactgtccc tgacaataaa
     5281 acagaggagg ccacgcttcc ttccttggca acaacaagat gaataggctc atcttttaga
     5341 gcagtctgag aaggttgtct aaagagtaaa atccccccag cacccaatac aactacactc
     5401 gcagcaccga ttgctgcata cagttgccac tttttagctt taccattctt tttcttcata
     5461 atgaaactcc ttttcttttt tacaatactt tgctattata ccaaatttcc ctccagcaaa
     5521 caatacagtt caggattaaa caatcgttcg gaattttgct tttctattgt actagatata
     5581 taatacacta tcttgactta tttggcaagc ttttttgcaa ttttttgtgc gtagcagatt
     5641 tttgcaatca aatcaaaaaa agaataaaat ataactatca aaaaagacgc tctactattt
     5701 aaaagattac aaaggagttt tcaatgagag aatatgatat cattgctatc ggtggaggta
     5761 gcggaggaat cgctaccatg aaccgtgctg gtgaacatgg agccaaagca gccgttattg
     5821 aggaaaagaa attaggtgga acctgtgtca acgtcggttg tgttcctaaa aaaatcatgt
     5881 ggtacggggc acaaatcgct gagactttcc atcaatttgg agaagactac ggctttaaga
     5941 ctactgatct taactttgac tttgcaaccc tacgtcgcaa tcgtgaagcc tacattgatc
     6001 gcgctcgttc ttcttatgat ggtagtttta aacgcaacgg tgtagacttg attgaaggtc
     6061 atgctgaatt tgtagattct catactgtaa gcgtaaatgg tgaactgatt cgtgctaaac
     6121 atatcgtgat tgctacaggt gcccatccaa gtattcctaa tattcctggt gctgagctag
     6181 gtggctcttc tgatgatgta tttgcctggg aagaacttcc agagtcagtt gccattctag
     6241 gcgctggtta tatcgccgtt gaattagctg gcgtactcca cacttttggt gtcaagacag
     6301 atctctttgt tcgccgcgat cgtcctttac gtggttttga ttcctacatc gttgaaggtt
     6361 tggtcaagga aatggaaaga acaaacttac cacttcacac tcacaaagtc cctgtcaagt
     6421 tagaaaaaac tactgacggc attaccattc atttcgaaga tggtactagt cacacagcta
     6481 gccaagttat ctgggctaca ggccgccgtc caaacgttaa gggcttgcaa cttgaaaaag
     6541 ctggagtgac tctgaatgaa cgtggcttta tccaagtaga tgaataccaa aataccgtcg
     6601 ttgagggaat ctatgctcta ggtgatgtaa cgggcgagaa agaactgact ccagttgcaa
     6661 tcaaggccgg acgtacccta tctgaacgtc tctttaacgg aaaaactact gcaaaaatgg
     6721 attactcaac tattccaact gttgtctttt cacaccctgc tatcggaact gttggtttga
     6781 cagaagagca agctattaaa gaatacggtc aagaccaaat caaggtttat aaatcaagct
     6841 ttgcatctat gtactctgct tgcactcgca accgtcaaga atcccgtttc aaactcataa
     6901 cagctgcttc agaagaaaaa gttgtcggac ttcacggaat tggctacggc gttgatgaaa
     6961 tgattcaagg atttgctgtt gctatcaaaa tgggagcaac caaggctgac tttgacgcaa
     7021 ctgtagcgat tcacccaact tcatctgaag aatttgtaac catgcgttaa tcgaaacaaa
     7081 agaagcaaag caaaaaacac ttctaaatat caaaaagcga gtatttccgt agttggagat
     7141 actcgttttc ttatgtttta ttttatagta tgttgaagta agatatgcaa aaatcaatta
     7201 acaaaccata
//
LOCUS       DAVMGZ010000096         6991 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000096 DAVMGZ010000000
VERSION     DAVMGZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.097"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(329..1171)
                     /locus_tag="VYQ93_001585"
     CDS             complement(329..1171)
                     /locus_tag="VYQ93_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV3336246.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(1247..1324)
                     /locus_tag="VYQ93_001586"
                     /pseudo
     CDS             complement(1247..1324)
                     /locus_tag="VYQ93_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(1427..2818)
                     /locus_tag="VYQ93_001587"
     CDS             complement(1427..2818)
                     /locus_tag="VYQ93_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV3336247.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKMEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEAAILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(3053..3430)
                     /locus_tag="VYQ93_001588"
                     /pseudo
     CDS             complement(3053..3430)
                     /locus_tag="VYQ93_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            3663..4640
                     /locus_tag="VYQ93_001589"
     CDS             3663..4640
                     /locus_tag="VYQ93_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV3336248.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFRKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            4637..5719
                     /locus_tag="VYQ93_001590"
     CDS             4637..5719
                     /locus_tag="VYQ93_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3336249.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     SEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(5783..6819)
                     /locus_tag="VYQ93_001591"
                     /pseudo
     CDS             complement(5783..6819)
                     /locus_tag="VYQ93_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         2064 a         1534 c         1201 g         2192 t
ORIGIN      
        1 gaaacttgaa atttatattt tgtatcaaat gatatttgag aaatttcttt caatacaatt
       61 acctccttga taaattctat atagtataaa attcaaaaag aaccaccttt caacacccaa
      121 gccttgcgac taggggcgtc agaaacgcgg ttccacccta atttattact tcattgtttt
      181 tgaaaatact acagaaagcg ccagctcatt attttgccct acttggctcc cactgtccca
      241 agctcgcttg aagaacgcca taagactttc tttcctgctg actattatat caaaaattag
      301 aataatgtac aattcttttt atgattttct agaaatccac atcatcaact cgtggagcac
      361 ccgattgaac agcaatcgtt tttaaggttt ctctctcctc atgactcagt tcaattccaa
      421 agcaatcaag attagcttga atacgagagg ttgtgacaga ttttggaagt ggtaaaaatc
      481 cttctgccaa gctccaggcc aaggctatct gagcaacaga ctttccgtga tttgctgcta
      541 tttcttggac ttgcttgcta tcaaacagtt ctccttgtcc aaaaggcccc caagcttcca
      601 ataaaattcc cttttcacga cagtaagcta cgacttcctc ttgatacaca cctggcgcca
      661 agcgaacttg attgaccgca ggaacgatag ttgcagtttc aagcaaggca tccaaatgat
      721 ggggaagaaa attgctaacg ccgatagcac ggattttccc ttcttgatag aggtcttcca
      781 tcgctctcca aacttccgca ttgcgagttt tccatgcgtc attttctctg agcggttttg
      841 ggttcggcca atgaatcaaa tacaaatcca aataatccaa gcccagtttt tctatagatt
      901 tttccaaagc ttgacgagtt tgctcatagg tttgctgact attccaaagc ttggtagtta
      961 cgaacatttc ttcacgtgga actccgctat ctttgattgc ttgaccaaca ctttcttcat
     1021 tctgataaat cgccgccgta tcaatatgac gataaccagc cttcaaggct tctaacactg
     1081 cacgataggc ttcttctcca tccttagcct taaaagttcc aaatcccaat actggaattt
     1141 ctactccatt atttaattga taggtattca tatgcttctc cttctatttt atactctctt
     1201 caaaccacat caacgtcgcc ttgccgtagg tattgttact gacttcgtca gttctatcca
     1261 caacctcaaa acactgtttt gagcaacctg cagctagttt cctagtttgc tctttgattt
     1321 tcattgagta ttattcatca attatagcat aaaacatttt ttaagtagta ctatcttttg
     1381 taaaaaataa gtctatcaca tatctgacag acctattttt tcttgcttat tcatcacgac
     1441 tataatacag ctcatgagct tttaggcgag tattgagcat ctctggtatg gtcacaaagg
     1501 tataaccttg atttttcaaa tactcaatga cccttggcaa ggcattgact gtcggactgt
     1561 gaatatcatg catcaaaacg atagagccgt tagctacttg atgctgaatt tccgtcaaaa
     1621 tagctgcttc atttttactc ttccagtcca gactatccac atcccacata ataaagctca
     1681 aatcgaggct attgcgaata tcatctgtaa tagctccata aggcggacgc atgagtttgg
     1741 aactagaacc cagcacttta gttagcacat cctcagtatc agtaatctgc tttttagctt
     1801 catcaagaga tagttgcgag agaataggat ggctccaact atggtttcca acaacatgac
     1861 cttcagattt tatcctcttc accaagtcct cattcccaga aacatttttc ccaagcacaa
     1921 agaaagtcgc tttaatatca tatttagcta gggtctctaa tacctgcggg gtcgttgctg
     1981 gatttggacc atcatcaaag gttagagcga caactttttg atgtttctta tcaaagtaag
     2041 attggtacaa ggccgcatct ttttcgagta agtacgaaga ttggataaca tcaaagaaag
     2101 cagatactgg caaggctatc tcttctaaat tttcaaccac aggacttgga taaaggataa
     2161 tctgactatc cttgtaatca aaattccatg cagacaagtc ttggtcagag aagtttttta
     2221 caatctgctc actctggtct tgctccattt ttttatcctc tatgaaggag gtcaactctt
     2281 ttatcagctg ttccttagcc ttactagcat ctgaaaacag ttggtcaagt gtaaaaggtt
     2341 gcccatcttc tgttaagtga acctttccta gactggtctt ttcagtctct tcaatcttaa
     2401 aagcaactaa atcatagatt tgtttggtca cattacgatt aacgactccc tttaaacctg
     2461 actcttcttg ctctgtatag tagaaaacaa gattgtcctt actttctagc ttgtccttaa
     2521 catcttgatt tatcagctcc ctaacagagg aaatcacttt ctccccttgg agaggataat
     2581 aggcaatcac ttcggcttgc ccctgacgaa aatgctcctt ctgatttccc tcactcaatt
     2641 gatcatcttt ctcttttttg agcgattcaa tcttttgctc aaaacttttt tgctggtaga
     2701 tcttaaaagc aataaatccg cctaataggc aaatagaaat tgctaaaata ccaatcaaag
     2761 ccaataatac atgtctcgtt ttcccgtgtc tgccacgtcc tagtctactt ttattcatat
     2821 ttatatcata acaaatttga gcaataattt caatgataca atttggcaat ccacaattcc
     2881 tctagcaagc caaagcatct tacatcaact ccaacatccg tatctgtaga gattatgtag
     2941 ttttctatct atggaatttc tagtagattg aagctagaat agtagaccta tacttctaaa
     3001 acattgttag aaatcgattt gactgtcctg atcgatttgt cctgttctca tttcatttta
     3061 ctataaaact atgaaaagct aggtcatctg agaagtaatt actatcgaca attgcgatag
     3121 cgtatactag tgcgatttgt ttgtagctct ggtgggtatc accttcacgt tggcgaattt
     3181 tttctaggtt ttgattaacc taactgcaca gataagccca taggcgattg atgaaaaaat
     3241 tctgatgatg aacctgaatt tcgatgataa cttggattcc attatctaac tccgccaaga
     3301 cgtctatact agtatagaaa tcctgcgctg agtagggcat ggaaggcaag acgtgaatgt
     3361 tacttccctc caaaatcgtc acattttttg ctggcaagtc cagcatatcg cggataaatt
     3421 gacaaatcat tagttgggct gatacccgga tgtctgagaa tcctcctttt cctccttcta
     3481 ttatagcaca tttttaaatc taggtttgct agattctatg cttctatcta tttattcgga
     3541 aaagaaagct tttcttcaga agaaagctaa tagcttattt gaaattttct cctctctgtt
     3601 ggatagtccc gtctatctat gttataatga aagaaggatt taaaatcgga aaaggagtat
     3661 ttatgctaaa attaggtgtc atcggaactg gcgctatcag ccatcatttc atagaagcag
     3721 cccataccag tggagaatac caactggtcg caatctattc tagaaaacta gaaactgcag
     3781 caacctttgc ttctcgctat cagaatatcc aactattcga tcaattagaa gtcttcttca
     3841 aatcttcttt tgatttagtc tatatcgcca gtccaaactc cttacatttt gctcaggcaa
     3901 aagctgcctt gtctgctggt aaacatgtta ttctcgaaaa gccagctgtc actcaaccac
     3961 aagaatggtt tgatttgatt caaacagctg aaaaaaataa ctgttttatc tttgaagcag
     4021 ctcgtaatta ccacgagaaa gcctttacta ctatcaaaaa ctttttagca gataagcaag
     4081 tgttaggagc agatttcaac tatgccaaat actcttccaa gatgcctgac ttgttgactg
     4141 gacaaacacc aaatgtcttt tcagaccgtt ttgctggtgg agccctcatg gatttgggga
     4201 tttatcccct ctatgctgct gttcgtcttt ttagaaaagc caatgacgcg acttaccatg
     4261 ctcaacagct tgacaatagc attgacctaa atggagatgg tatcctcttc tacccagact
     4321 atcaagttca catcaaggct ggaaaaaaca tcacttccaa tcttccttgc gagatttata
     4381 caacagatgg aaccttgact ctcaacacga ttgagcatat tcgctcagct atttttaccg
     4441 accaccaagg aaatcaagtc cagcttccta tccaacaggc tcctcatacg atgactgagg
     4501 aagtcgctgc atttgcacac atgatccagc aaccagatct gaatctctac caaacttggc
     4561 tgtatgatgc aggatctgtt catgagctac tatataccat gcgccagact gctggcatta
     4621 gatttgaggc agaaaaatga aaaccaaact accgactgaa tggcaagaac tgagtgacca
     4681 actcggtttc caagaattca cccccattca aactcaacta tttgagcccc ttcttgctgg
     4741 agaaaacctc ctaggagtga gccaaaccgg aactggtaag accctagcgt atctcctacc
     4801 aagtcttctc agactacaaa agaaaaaagc ccaacaactc ttgattctag caccaaatac
     4861 ggaacttgct ggacagattt ttgatgtatg taaaacgtgg gcagaagcta tcggattgac
     4921 tgctcaactc ttcctttcag gttcaagtca gaaacgacaa attgaacgcc ttaaaaaggg
     4981 gccagaaatt ctgattggaa ctcctggccg tatctttgaa ctcattaaac ttaaaaaaat
     5041 caagatgatg aatgtggaaa ccatcatcct ggatgaattt gaccaattac tagatgattc
     5101 tcagattcac tttgtagaaa aaatcactca ctacgcacct cgtgaccacc aactggtcta
     5161 catgagtgcc acgaccaagt ttgaccaaga aaagattgtg ccgaatacac gcactattga
     5221 tctctctgac caaaaattgg acaatatcca gcatttctac atgcaggtag accaacgtca
     5281 tcgagtggat atgctacgaa aactggctca tgtagaggat ttccgtggtc tagtcttctt
     5341 caatagcctg tcagaccttg ggaatgccga ggaaaaacta cagtatcgtg atatattgac
     5401 tgtttccctc gctagtgatg ttaatgttaa atttagaaaa atcatcttag aaaagtttaa
     5461 agataaccaa ctaaccctgc ttcttgcaac tgaccttttg gctcgtggaa ttgatatcga
     5521 tagcctagaa tgtgtcgtaa actttgatat tcctagagat agcgaaacct acacacaccg
     5581 tgctggccgt actggtcgta tgggcaagga aggatatgtt atcactctcg tcactcattc
     5641 agaagaaatc aaaaaactta aaaagtttgc aagtatccga gaaattgtcc taaaaaacca
     5701 agaactctat atcaaataaa gccccattgc tctatacaca tccagtgtat ggagcttttt
     5761 tataagcaat tcaattttat actcatctgc tttcaaaaag cattctagtc catctccgat
     5821 taacgatggg ctttatcacc tccttctcca gtccttgtat gatatcttga agttgattca
     5881 tgacatcttc caaagtttga aaggctttat tcttaaatcc acgtttacga atctctttca
     5941 acacttgttc aatggggttc atctctggtg tgtatggagg aataaaggta aaaccaatat
     6001 tagtcggaat ctttaaggta cttgatttat gccatatagc attgtccata acgaataaaa
     6061 gataatcatc tggataagct tgtgaaagct cttctaaaaa gacgttcatc cactcagtat
     6121 tacatccacc agctattaag aaaaatgatt cgcctgtatg ggcatcaaca gctccataac
     6181 aatagcgaaa ttctcgtata tagtgactat ggacatgtgg acctactcct attggagacc
     6241 aacaagatcc cagtttactg attctaccga aaccagcctc atcttgggac atcaagtgaa
     6301 ccttacgaaa acgtctactg gttttaaatc gctttctttt cttcttgaat tgagatttta
     6361 tttttagacg cgacaatggt ttgagcgtct gctttcttag gatgttctgg acgtggcgta
     6421 atatttcgcc aaccatggcg cttcaacaat tgatagaagg catcacgtgt gtaggaacga
     6481 cctaactcct ttttataagc ctgaaataag gcatcaattg taacaaattc tcctgcctct
     6541 gcagccttca aatggcgggc aagaaagact ttcttttcct caactgtcat atatgcatgg
     6601 ttacgaccac cacgtgtttc ttgaaggaga gagtcgagtc cgaactcctc atattttttt
     6661 acattttgct aaatcgttgt ttgattacag tctaaaagct ctataatctc tttataagat
     6721 ttgcccatca gacgaaatag aacgatttga aggcgtttat gatatttagc tgtacgagag
     6781 tgttttgatg gtttggattt cttctttagt tgatttcata tcactattgt ataatgcttt
     6841 ttgattttag tctagtatta tactcttcga aaatctcttc aaaccgcgtc agcttcgcct
     6901 tgtcgtatag atgttactga cttcgtcagt tctatctaca acctaaaaac agtgttttga
     6961 gcagtctgta gctaactttc tatagtaaaa t
//
LOCUS       DAVMGZ010000097         6804 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000097 DAVMGZ010000000
VERSION     DAVMGZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.098"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            20..2293
                     /locus_tag="VYQ93_001592"
     CDS             20..2293
                     /locus_tag="VYQ93_001592"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV3336250.1"
                     /translation="MRFNQYSYINFPKENILSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSENELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSSQDVIREVVYVESRVDTDQKGLPDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVSLPHKIELEKPQLNLVQPQGKAELVAEAEEKLTHINSSYTLNDY
                     FLPRGFATLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNDRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            2310..2780
                     /locus_tag="VYQ93_001593"
     CDS             2310..2780
                     /locus_tag="VYQ93_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV3336251.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(2897..3097)
                     /locus_tag="VYQ93_001594"
     CDS             complement(2897..3097)
                     /locus_tag="VYQ93_001594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336252.1"
                     /translation="MKIKEQTRKLAASCSKHCFEVVDKTDEVSSKHGFEVVDETDEVS
                     NHTYGKATLTRIEEIFEEYKSS"
     gene            complement(3368..6718)
                     /locus_tag="VYQ93_001595"
     CDS             complement(3368..6718)
                     /locus_tag="VYQ93_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV3336253.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
BASE COUNT         2101 a         1483 c         1155 g         2065 t
ORIGIN      
        1 cattctatat ggaggttcta tgcgttttaa tcaatatagt tatatcaatt ttccaaaaga
       61 aaatatctta tctgaactaa aaaaatgtgg ctttgattta caaaatacag ccaatcacaa
      121 agactcactt gaaacctttt tacgtcgttt ctttttcact tatcaagaca ccaactaccc
      181 actttccatc ctagcagctg ataaaaaaac agacctgctg acattttttc aatcggaaaa
      241 tgaactgaca gcagatattt tttatactgt tgcttttcaa cttttaggct tttcttattt
      301 ggttgacttc gaagatagtg acgtttttcg taaagaaact ggatttccca ttatatatgg
      361 tgacttgatt gaaaatctct atcagttact caatactcgc accaaaaaag gaaatactct
      421 tatcgaccaa cttgttagtg atggtcttat tccggaggat aatgactacc actactttaa
      481 cggcaagagt ttggctactt tttctagcca ggatgtcatt cgtgaagtcg tttacgttga
      541 gtctcgtgtc gatactgacc aaaaagggct accagaccta gtaaaggtta gcattattcg
      601 ccctcgtttt gatggaaaaa tccctgccat catgacagcc agcccttatc atcagggaac
      661 caatgataag gctagtgaca aggctctcta caagatggag ggcgagcttg aggtttctct
      721 tcctcacaag attgagctag agaaacctca actaaatctc gtccaacccc aaggtaaagc
      781 tgagcttgta gcagaggctg aggaaaagct aactcacatc aactctagct atacactaaa
      841 cgactacttc cttccgagag gctttgccac tctctatgtc tcaggtgttg gtaccaaaga
      901 ctcgacaggt ttcatgacta atggagacta ccagcaaatc gaggcttata aaaatgtcat
      961 cgattggctt aacgaccgtt gccgtgcctt tactgatcac acgcgccagc gtcaagtcaa
     1021 ggctgattgg tcaaacggaa aagttgctac aacgggactt tcctatctag gtaccatgtc
     1081 caatggtctt gcgactacag gagtcgatgg tttagaagtt atcattgccg aggcaggaat
     1141 ttcatcatgg tacaactact accgtgaaaa cggtctggtg actagcccag gtggttatcc
     1201 aggtgaggac tttgactccc ttgctgagtt aacctattct cgtaatctct tagctggcga
     1261 ctatatccgt ggcaatgaag ctcaccaagc tgacttagaa aaagtgaaag ctcagctaga
     1321 ccgaaaaact ggggactaca atcagttttg gcatgaccgc aattatctgc tcaatgccca
     1381 taaagtaaag gcagaggttg tctttactca tggttctcag gattggaatg tcaaaccact
     1441 tcatgtttac cagatgttcc atgctcttcc tactcatata cacaagcatc tctttttcca
     1501 taatggtgct catgtttaca tgaacaattg gcaatcaatt gacttccgtg agtccataaa
     1561 tgccttattg accaagaaat tactaggaca ggaaacagac tttcaacttc ctactgttat
     1621 ctggcaggac aatacagctc cacagacttg gttatcactt gataacttcg gtgggcaaga
     1681 aaactgtgaa accttctcac ttggtcaaga agagcaagct attcaaaacc agtacccaga
     1741 taaggatttt gagcgctatg gtaagacata ccaaaccttc aatacagagc tctatcaagg
     1801 gaaagccaat cagattacta ttaaccttcc tgtgactaaa gatctccacc taaacggtcg
     1861 cgctcagctc aatcttcgta tcaaatccag tacaaacaag gggctcttat ctgcccaact
     1921 gctagaattt gggcaaaaga aatacctaca accttatcca gctattttaa gtgctagaac
     1981 cattgataac ggtcgctatc acatgttgga aaatctctgt gaattgccat ttagaccaga
     2041 ggcacaacga gtcgtgacaa aaggttacct taatttacaa aatagaaatg atttactgtt
     2101 agtagaggat attactgcag atgaatggat ggacgttcaa tttgaactgc agccaactat
     2161 ttacaagcta aaagaaggag acactctccg tttagtcctc tatactactg actttgaaat
     2221 caccatccgt gacaatacag actaccactt gactgtcgac ctcgctcagt ccatgcttac
     2281 cttaccttgc taaaaggagt tacattatta tgaataaatc agaacaccgc caccaactta
     2341 tacgcgctct tatcacaaaa aacaagattc atacacaggc tgagttgcaa gcccttcttg
     2401 ctgagaacga cattcaagta acccaggcaa ccctctcacg cgacatcaaa aatatgaacc
     2461 tatcaaaagt ccgcgaagaa gatagcgctt attatgttct taacaatggt tccatctcaa
     2521 aatgggaaaa acgtctcgaa ctctacatgg aagacgccct tgtctggatg cgcccagttc
     2581 aacaccaagt cctactaaaa acccttcctg gactggctca atcctttggt tctatcattg
     2641 atactttgag cttccctgac gctatcgcta ccctttgtgg taatgatgtc tgtcttatca
     2701 tctgtgaaga tgcagatact gctcaaaagt gctttgaaga actgaaaaaa ttcgccccac
     2761 catttttctt tgaagaataa taaatgacta caactaacaa ttatgctttt aaaaaacctg
     2821 gtagaaagtc tttaacatca gaaaacgcat agtatcaagt gctgaaaacc cttgatacta
     2881 tgcgttttgc tttatcctag gaagatttat actcttcgaa aatctcttca atccgcgtca
     2941 acgtcgcctt gccgtaggta tggttactga cttcgtcagt ttcatctaca acctcaaaac
     3001 catgttttga gctgacttcg tcagtcttat ctacaacctc aaaacagtgt tttgagcagc
     3061 ttgcagctag tttcctagtt tgctctttga ttttcattga gtattatttc attttctcct
     3121 gcaattgaat tcttgctcgg ctttttgttt tctatttctt taaaatcaaa gtagctcttt
     3181 tgttaataac tcgatcaaca aacatcgtgg tacaagtatc tactttgaaa tttatcaacc
     3241 acttaacaac tgatactgta ttctaggaaa acgatgacat tcttcctaat aaaacttctc
     3301 atatatagca taaatttcta ctctttttaa ttttaaatga gtctcagcat ggcaatcact
     3361 ttagaattta agccgcttct ccagctcgat tattgataag ttcaatgatt tgaacaatat
     3421 tacgaagtac tggtagttgg tgttggaaga gtagttgaac agaaccataa gatttaaacg
     3481 gctcttgcgt taacactttc gtctctaaaa aaccattttc tacaatgtag tctacaatca
     3541 attttacaaa tgaaatctgc ctggcattaa gattctcatc cgacaaaaat ttagaaaaaa
     3601 tacgattggc agactctcta tctaagccaa taatctcacg aaccaatctc ggaattgcct
     3661 tattttcata atgactttgg tagtctgctt tactacctaa tttttcccaa agcaattttt
     3721 caagtgcaag catatcatca gatgtcaact ttttattatg gtatagctta gaaatggact
     3781 cctcatccag atgagttttc aaataatgct caactttttc attgtaagac cgaagatcgt
     3841 tgacctgcaa aaatgctgtg gtctcgtgaa cagtggagag tatacgatct tcaaagttaa
     3901 catagtaggg tttacggtct gttttatcca aaaactgtaa taaatctcga atagcaagac
     3961 gaattttttc caaatcagac aagttaactt ctttccaaaa ttcaggctcc tgtactttcc
     4021 tgataatttc agcctgctca aaaacctgcg ggatattgcc aatagcagaa agagctctag
     4081 ccgtcttcat cacttgggaa atatgaacag tggaagattt agctgtcagt tgccccaact
     4141 gaatatgaag caaccacaaa tcaaatctcc tcgccatctc atcttcttta tcttcatcaa
     4201 ataaaagcag agagagattt ttttgaatgg tttcacttgt aacagcagtt agattctgcc
     4261 aactttccaa tttcctatag ctataaactg tatctaaaac catacgaacc ctgaagtcca
     4321 actcatttaa gctctctata cgaccttgaa gttccgagac aagctgctga cggtatgctc
     4381 tcgcaaactg atcttcttgg tattggagtc cctgaagttc tcgaatcaag tccactttga
     4441 tattaaataa acgctgagtc aacgaaacaa tgtgacgacc ctctccatct cttggatctg
     4501 cacgaaaata atcaaaattg tccccataat caaataccaa gaagttttcc ttatcctgct
     4561 caggtccaaa taaatcttta catagacggg ttcctcgacc aatcatctgc caaaacttag
     4621 ttttagagcg tactttcttg aagaagacta aattaacaac ctctggtaca tcaatacctg
     4681 tatctaacat atcgacagaa atcgcaattt gaggatactt ctccttaatt ttaaaatcat
     4741 caatcaaggt ctgataatgc ttaatactat aatcaatcac ctgagcatag tcgctccctt
     4801 tttcaggata gcggttgtta aaaatacctc tgatatattc cgcatgatca tgatttttag
     4861 caaaaataat agttttacca atttcatcac ccgaggctgt ctgaattcct cttgtcatga
     4921 gttcatttaa aacaatttct actgtacttt tattgaaaac aaaggaatta aatacactcc
     4981 catcaatatc tttttcacag ctatcgtctt caaatttgct atcaaaatgt tccttttctt
     5041 cttcggacaa atcatcataa tgtagaccat ccgtaggtag tttcagtttg gtttcgatag
     5101 aatgataggc tactaaatat ccgtctttaa cagcctcttc caaatcatat gcatatgttg
     5161 gaaccccatt ctccaaatta aagaatccat aggtgttttt atctaaatct tgacgcggag
     5221 tagctgttaa gcctacaatt cttgcatcaa aataatcaaa aatggacttg tatttctgat
     5281 aaatagaacg gtgagattcg tcaattatga taaggtcaaa atgcccaaca gtgaaagggc
     5341 gttgatttac ttcttcttga ccactaatcg ctccaatcat ggtcggataa gttgaaaaga
     5401 ccatgcgact tgattgagct ccttctttat cttctaagaa gttacaaacg gaaagatctg
     5461 ggagtaattt tctaaacgaa tcataggctt gcttaaccaa ggaagttcta tcggctaaga
     5521 agagaacgtt ttttacccag ttatgacgtg ataagatatc aactagagaa actgctgtac
     5581 gagttttccc agccccagtt gccataacca aaagtgcctg tctacgatga ttagagaaag
     5641 cttcacaaac gcttgcaatg gcatgttttt ggtagtgacg cccggaaata tcgtccctaa
     5701 ttttactaga aatatcctca agtggttttt gaagatgacg tctatccatc actaattgca
     5761 attcttcttg agagtaaaag cctgcaatct ggcggcggtt cggaccatca agtatatagt
     5821 gcttcaagcc atttgtaata aagcaaattg gctgatagcc gatatgtttc tcaagagctt
     5881 cagcatattc tttgacctgt acttccccta cttctggatt gacagaggct tttttagcct
     5941 ctacaatcgc taaaattttc ccatttttac cataaagtac atagtcacag taaccaattc
     6001 ctgaaccgtg cttgagacca tcaacggcta tctcaacacg acagttttct tcaaatatcc
     6061 atcctgctaa acggagatcg atatcaataa agagctttct tgtctctgcc tcagataatt
     6121 gatcaatatg gaaaggcaca tcttgccgtt gcgctgcagt ctcacgtttt tcagtataag
     6181 tctcttgaac ttctacggac tgagcagcca tctgttcatg aaaatcaggc agttctggta
     6241 aactgtcttg taactttatc atagattgtg gagtttctcg gtatttgatg tttgctgaaa
     6301 gtggtaagca cttctcatca aaataacgct ccacaaactc attgctgtaa caataatcga
     6361 taaaattaac aaactgatac aaatgatgca aagctaaaat cgcttctcgt tccttaattt
     6421 caccaccatg agcagcatgg tttccccacc gaatcaacag aactatctgc ttgtggagtt
     6481 cagaatctac gatatcccta aaatcatcat cccatactaa agaagataga gtagcacgat
     6541 agggagcttc taaatatgaa tcgtgactat atatccaatg gacagcctgc tctaaagcac
     6601 gacgcgccat aaaggcagta gccacagttg atgtagcaat catattctca gcttcaatgc
     6661 aaggttttga aaaagattca tattcgtcat tttttaaaag aaagctaaaa tttcccattt
     6721 ttttctcact ttaaacaaat agtttagcta tattatacta aatttcaagc tatttctcca
     6781 cacttttata gctatctcac aagc
//
LOCUS       DAVMGZ010000098         6776 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000098 DAVMGZ010000000
VERSION     DAVMGZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.099"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            469..1830
                     /gene="trpE"
                     /locus_tag="VYQ93_001596"
     CDS             469..1830
                     /gene="trpE"
                     /locus_tag="VYQ93_001596"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV3336254.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVINPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            1827..2393
                     /locus_tag="VYQ93_001597"
     CDS             1827..2393
                     /locus_tag="VYQ93_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV3336255.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            2404..3408
                     /gene="trpD"
                     /locus_tag="VYQ93_001598"
     CDS             2404..3408
                     /gene="trpD"
                     /locus_tag="VYQ93_001598"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV3336256.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            3405..4172
                     /gene="trpC"
                     /locus_tag="VYQ93_001599"
     CDS             3405..4172
                     /gene="trpC"
                     /locus_tag="VYQ93_001599"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV3336257.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENRAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALAEERLKELYDYATVLGLEVLV
                     ETHNLAELEVAHRLGAQIIGVNNRNLTTFEVDLQTSVDLAKHFKDDCLYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAEDVAQRIKELQIDKG"
     gene            4159..4758
                     /locus_tag="VYQ93_001600"
     CDS             4159..4758
                     /locus_tag="VYQ93_001600"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV3336258.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAV
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            4736..5959
                     /gene="trpB"
                     /locus_tag="VYQ93_001601"
     CDS             4736..5959
                     /gene="trpB"
                     /locus_tag="VYQ93_001601"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV3336259.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            5952..6728
                     /locus_tag="VYQ93_001602"
     CDS             5952..6728
                     /locus_tag="VYQ93_001602"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV3336260.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLGETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLANTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(6700..>6776)
                     /locus_tag="VYQ93_001603"
     CDS             complement(6700..>6776)
                     /locus_tag="VYQ93_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV3336261.1"
                     /translation="ERLPFVPFLLLAACVIIFGKLLLV"
BASE COUNT         1947 a         1375 c         1698 g         1756 t
ORIGIN      
        1 agatagaact gacgaagtca gtaaccatac ctacggcaag gcgacgttga cgcggtttga
       61 agagattttc gaagagtata aaggtgtacc gagatgaaca tctcttcatc aatgtctctg
      121 gtcgtggagg gaaggaccta gctgcgattg cagactacta gaagctaaaa aataatagat
      181 ggaaaaatta cagttctttc tctcacagag agcttgtggt tgctgaaaac aagcagagaa
      241 agctgtaaag ttgggttcat ctgaaacgag agaagaaaac atcattctca agggagtgcc
      301 ctttatcgca cagcctgtta caggagatat ctgagacagg aatgagagat aggagaaatc
      361 ctataattga ggtggcaccg cgaatttcgt cctcacgcaa gttattttgc gtggggattt
      421 ttcatacagt cgctacgaac tagaattcga actgaaaggg aaattacaat ggaacgaatc
      481 attcatggag atgtcttatc accaatcttg gcttatatgc gcttaaaggg gcaacacaag
      541 gttatcttag agagtattcc gagagacaag gaaacagctc gtttttctat cctagcctat
      601 aatccagttt ttgagattaa gtttgaaaat ggagttctct atcaaaatgg tcaagtgatt
      661 gatcgggatc ccttggattt cctttatgaa gtgattcata agagtcagca ccactcagag
      721 ctcccttttg gtggtggagc cattggtttt gtgggttacg atatgatttc gctttatgaa
      781 gaaattggtc aaatccctga ggatacaatt gggacgccag acatgcattt ctttgtctat
      841 gagagctata tggtctttga ccacaagaag gagaagatac atgtcataga ggatgcactt
      901 tatagtgagc gcagtcaaga ggccttggaa aaatccttga accaagtgct tgaggagtta
      961 cgcattcctg ctccaaatga atttgaagac ttggatctat ctccgttaga cttcaaaccc
     1021 catatcgctc ctcagaagtt tgaggaaatg gtggaaaccg ctcgcgactt gattcgtaac
     1081 ggagatatgt tccaatgtgt actcagtcag cgcttctcag cagaagttac tggaaatcca
     1141 tttgacttct acagaaatct ccgcgtgatt aatccttcta attaccttta tttctatgat
     1201 tttggggatt atcaaatcat cggagctagt ccagaaagtt tggtttctgt caaaaatggc
     1261 atcgtgacaa ccaatccgat tgcagggacg cgaccaagag gggctacgga tgaagaagac
     1321 aaggccttgg cgactgacct cctctctgat gagaaggaaa cagcagaaca tcgaatgttg
     1381 gtagacttgg ggcgtaacga tattggccgc atctctgaaa cggccagtgt ccaagttact
     1441 aagtatatgg aggtggagct cttccgctac gtcatgcatt tgaccagtgt ggtcaaggga
     1501 cgtttgcttg cagaactcac tgccatggat gccttgaaag caacgcttcc tgctggaacc
     1561 gtttctggag caccaaaaat tcgagctatg agacgcatct atgaactgga aacggaaaaa
     1621 cggagcgtat atgcaggagc aatcggctac ttgtctgcga cgggtgatat ggatttggcc
     1681 attgccatcc gaaccatgat tctcaaaaat caaagagcct atgtgcaggc tggggcaggg
     1741 attgtctacg actctatcgc ccaaaacgaa taccaagaaa ccattaacaa agcaaaatct
     1801 atgactagaa ttggagaact aagaccatga ttttattgat tgacaactat gattctttta
     1861 cctataactt ggcccaatac attgggaatt ttgcagaagt tcaggttctg agaaatgatg
     1921 attccaagct gtatgaagaa gctgaaaaag cagatggtct ggtcttttct cctggtcctg
     1981 gttggccagt tgatgctgga aagatggaag acatgattcg tgattttgca ggcaagaagc
     2041 cgattctagg gatttgtttg ggccaccaag ccattgcaga agtctttggt ggtaagctag
     2101 gtttggctcc aaaagtcatg catgggaaac agagcaatat caactttgaa gcgccatctg
     2161 ttttgtatca aggtattgag gatggccgtg cggtcatgcg ttatcacagt attttgattg
     2221 aggaaatgcc agaagacttt gaagtgacag ctcgttcgac tgatgaccaa gccatcatgg
     2281 ggattcaaca taaaaaccta ccgatttatg gcttccagta ccatccagag agcattggaa
     2341 cgccagacgg cttgtcttct attcggaatt ttatcgagaa ggttgtaaag tgaggaaact
     2401 aggatgaaag agattattga aaaactagca aaatttgaaa atttatcagg tgtggaaatg
     2461 acggatgtca ttgagcgtat cgtaactggg cgtgtaactg aagcgcagat tgcttctctc
     2521 ctcttagctc ttaagatgaa gggggaaaca cctgaagaac gcacagccat tgcccaagtc
     2581 atgagaggac atgcccagca tattccaact gagattcatg atgccatgga caactgtggt
     2641 acaggtgggg acaagtcttt cagttttaat atttccacaa ctgcagcctt tgtcttggct
     2701 ggtggcggta ttcacatggc caagcatggt aaccgctcga tttcttctaa atctggttcc
     2761 gcagatgtcc tcgaagcctt gggaatcaat cttgacctca aaccagctga actaggtaag
     2821 gtctttgata aaactggaat cgtctttctc ttcgctaaaa atatgcaccc agctatgaaa
     2881 tacatcatgc cagctcgttt ggaattggga attccaacga ttatgaactt gactggtcca
     2941 ctgattcatc caatggcttt agaaacacag cttcttggaa ttagtcgtcc agaactccta
     3001 gaaagtacag ctcaggtttt gaaaaatatg ggtcgcaaac gtgccatcgt ggttgctgga
     3061 ccagaaggat tggatgaagc tggcttgaac ggaacaacca agattgcact tcttgaaaat
     3121 ggcgaaatca gcttgtcaag ctttactcca gaggatttgg gaatggaagg ctatgctatg
     3181 gaagatattc gtggtgggaa tgctcaggaa aatgcagaaa ttttgcttag cgttctgaaa
     3241 aacgaagcaa gtccattctt ggaaacgaca gtcttgaatg ctggtcttgg tttctatgct
     3301 aatggtaaga ttgatagcat caaggaagga gttgccttgg cccgtcaagt gattgctaga
     3361 ggcaaggccc ttgaaaaact cagactgtta caggagtacc aaaaatgagt caggaatttt
     3421 tagcacgaat cttagagcag aaggcgcgtg aggtggagca gatgaagctg gagcaaatcc
     3481 agcctctgcg ccagacctat cgcttggcag aatttttgaa gaatcatcag gaccgcttgc
     3541 aggtaatcgc tgaggtcaag aaagctagcc ctagtttggg agatatcaat ctcgatgtgg
     3601 atattgtgca acaggcccag acttatgaag aaaacagagc agtgatgatt tcggtgttga
     3661 cagatgaagt tttctttaaa ggacatttgg attatctacg ggagatttcc agtcaggtag
     3721 agattccgac gctcaacaag gactttatca tcgatgaaaa gcaaatcatc cgcgctcgca
     3781 atgcaggtgc gacagtcatc ttgctcattg tggcagcctt ggcagaagaa cgtcttaagg
     3841 aactttacga ctacgcgaca gtgcttggtc tggaagtctt ggtggaaact cacaatctag
     3901 ctgaactaga agtggctcac agacttggtg cccagattat cggggtcaat aaccgcaact
     3961 tgaccacctt tgaggtcgac ttgcagacca gtgtagactt ggctaaacat ttcaaagatg
     4021 attgcttgta catttctgaa tctgctattt tcacagggca ggatgcggaa cgactagccc
     4081 catactttaa cggaatttta gtagggacag ctctcatgca ggcagaggat gtggcccaga
     4141 gaatcaagga gttgcagatt gacaaaggtt aagatttgtg gactatcgac caaagaagcg
     4201 gtggaaacag ccgtttcagc aggagccgac tatatcggtt ttgtctttgc acctagtaaa
     4261 agacaggtga ctttagaaga ggcagctgtg ttggcaaagc ttattcctgc agatgtgaaa
     4321 aaggttggag tatttgtttc accaagtcgg gtagaactgc tggaagcgat tgacaaagtt
     4381 ggcttggact tggttcaagt tcacggtcag gtggcagatg atttatttga gaatttgcct
     4441 tgtgccagta ttcaggctgt gcaggtagat ggaaatgggc atgtccccaa ttctcaggca
     4501 gattatctac tctttgatgc ccctgtggca ggaagtggcc agccctttga ttggggtcaa
     4561 ctggatacga ctggactagc acagcccttc tttatcgcag gtggccttaa tgaagataat
     4621 gtagtaaaag caattcaaca ctttactccc tatgcagtag atgtatcaag cggagtggag
     4681 acagatggac aaaaagatca tgaaaagatt agaagattta tagagagggt aaagaatggc
     4741 atatcaagaa ccaaataaag atggatttta cggaaaattt ggcggacgtt ttgtcccaga
     4801 aacattgatg acagcagttt tggagttgga gaaggcctac cgtgaaagtc aggcagaccc
     4861 aagtttccaa gaggaattaa accaactttt gcgccagtat gtgggacgtg aaactcccct
     4921 ttactacgca aaaaatttga cccagcatat cggcggagcc aagatttatc tcaaacggga
     4981 agaccttaac catacaggag cccacaagat taacaatgcc ttaggacaag tttggcttgc
     5041 caaacgcatg ggtaaaaaga aaattatcgc agaaacgggt gctggtcagc acggtgtggc
     5101 aactgcaact gctgcggccc tctttaacat ggaatgtacc atctacatgg gtgaggaaga
     5161 tgtcaaacgc caagccctca atgtcttccg catggagctt ttgggagcca aggtcgaggc
     5221 tgtaacagat ggttcgcgcg tgctcaagga tgcggtcaat gcagcccttc gttcatgggt
     5281 ggctaatatc gacgataccc actatatcct tggttctgcc ttggggcctc atccatttcc
     5341 agaaatcgtt cgtgacttcc aaagtgtcat cggtcgagag gctaaacaac agtaccgtga
     5401 cttgacaggt cgagatctgc cagatgccct agtagcctgt gttggtggtg ggtcgaatgc
     5461 tatcgggctc ttccatccct ttgtagaaga tgagtcagta gctatgtatg gaactgaagc
     5521 agcagggctt ggtgtggata cagagcacca cgcagctacc ttgaccaagg gtcgtccagg
     5581 tgtccttcac ggttccctca tggatgtgct ccaagatgcc catggtcaaa ttcttgaagc
     5641 cttctctatc tcagcaggtt tggactatcc tggtatcggt ccagagcatt ctcactacca
     5701 cgatattaaa cgtgccagct atgtccctgt gacagacgaa gaagccttgg aaggattcca
     5761 actcttgtct cgtgtggaag ggattatccc agccttggaa tctagccatg ctatcgcctt
     5821 tgcggtgaaa ttggccaaag aacttggacc agaaaagtct atgattgtct gcctatcagg
     5881 tcgtggggac aaggatgtgg ttcaagtcaa ggaccgcttg gaagcagatg cagcaaagaa
     5941 gggagaagct catgcctaag acactaacag aaaaattaaa tgctattaaa gcagctggaa
     6001 aaggaatttt tgttccctat atcatggctg gtgaccacga gaaaggtttg gatggactag
     6061 gagaaacaat ccacttttta gaagatttgg gtgtctctgc aattgaagtg ggtattccct
     6121 tttcagaccc tgttgcagat ggacctgtta tcgaagaagc tggcttgcgc agtttagccc
     6181 acgggacctc tacccaggct ttagttgaaa ccttgaaaac cattgaaaca gagattccac
     6241 tggtcatcat gacctacttc aaccccctct ttcagtacgg tgtggagaac tttgtcaaag
     6301 atctggctga tacagctgtg aagggcttga ttatcccaga cctgcctcat gaacatgcca
     6361 actttgtaga accatttttg gcaaatacag atatcgcctt gattccttta gtaagcttga
     6421 ccacaggaat tgagcgccag aaagagttga ttgaaggggc ggagggattc atctatgccg
     6481 ttgccatcaa tggagtgaca gggaaatcag gcaattaccg tgcagatttg gacaagcact
     6541 tggcgcaact tcatcaagtg gccgacatcc cagtcttgac aggttttggt gtatctagtc
     6601 aagccgatct ggaacgcttc aatgcggtgt cagatggtgt tatcgtcggt tcgaaaatag
     6661 taaaagctct ccaccaagga gagccgattc aggactttat caaacaagca gtagcttacc
     6721 aaaaataatc acacaagcag caagtaagag gaaaggcacg aaaggaagtc tttcct
//
LOCUS       DAVMGZ010000099         6622 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000099 DAVMGZ010000000
VERSION     DAVMGZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.100"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..918
                     /gene="glyQ"
                     /locus_tag="VYQ93_001604"
     CDS             1..918
                     /gene="glyQ"
                     /locus_tag="VYQ93_001604"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV3336262.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            1130..3166
                     /locus_tag="VYQ93_001605"
     CDS             1130..3166
                     /locus_tag="VYQ93_001605"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV3336263.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            3208..3465
                     /locus_tag="VYQ93_001606"
     CDS             3208..3465
                     /locus_tag="VYQ93_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV3336264.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(3688..4932)
                     /locus_tag="VYQ93_001607"
     CDS             complement(3688..4932)
                     /locus_tag="VYQ93_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV3336265.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(4951..5556)
                     /locus_tag="VYQ93_001608"
     CDS             complement(4951..5556)
                     /locus_tag="VYQ93_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV3336266.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFSDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            complement(5616..6539)
                     /locus_tag="VYQ93_001609"
     CDS             complement(5616..6539)
                     /locus_tag="VYQ93_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV3336267.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
BASE COUNT         1960 a         1294 c         1457 g         1911 t
ORIGIN      
        1 atgtctaaaa aattgacttt ccaggaaatc atccttactt tgcaacaatt ttggaatgac
       61 caaggttgta tgcttatgca ggcttatgac aacgaaaaag gtgctggaac aatgagtcct
      121 tacactttcc ttcgtgctat cggacctgag ccatggaatg cggcttatgt agagccatca
      181 cgtcgtcctg ctgacggtcg ttatggggaa aaccctaacc gcctttacca acaccaccaa
      241 ttccaagtgg ttatgaaacc ttctccatca aatatccaag agctttacct tgagtctttg
      301 gaaaaattgg gtatcaatcc tttggaacac gatattcgtt tcgttgagga caactgggaa
      361 aatccatcga ctggttcagc tggtcttggt tgggaagttt ggcttgatgg gatggaaatc
      421 actcagttta cttatttcca acaagtcggt ggattggcaa ctggtcctgt gactgcggaa
      481 gttacctatg gtttggagcg tttggcttct tacattcaag aagttgactc tgtctatgat
      541 atcgagtggg ctgatggtgt aaaatacgga gaaatcttta tccagcctga gtatgagcac
      601 tcaaaatatt catttgaaat ttcggaccaa gaaatgttgc ttgaaaattt tgataagttt
      661 gaaaaagaag ctggtcgtgc attagaagaa ggcttggtac accctgccta tgactatgtt
      721 ctcaaatgtt cacatacctt taatctgctt gacgcgcgtg gtgccgtatc tgtaacagag
      781 cgtgcaggct atatcgctcg tatccgtaac ttggcccgtg tcgtagccaa aacctttgtc
      841 gcagaacgca aacgcctagg ctacccactt ttggatgaag aaacaagagc taaactccta
      901 gcagaagacg cagaataaag agagtgacaa attacgaaaa tgggcgaaca gagtgagccc
      961 tgagccagtt gccgcagtga tgaaggtatc cttagtgaaa ctaaggatac taggcaaaat
     1021 tggagacttt tggctccaat tttagcaatg aaacaacgaa gttggttgct tgcgtgccaa
     1081 tcacataagg caaactggaa aataaaaaga tacttttcgg agaaaaaaca tgacaaaaaa
     1141 cttattagta gaactcggtc ttgaagaatt accagcctat gttgtcacac caagtgaaaa
     1201 acaactaggc gaaaaaatgg cagccttcct caaggaaaac cgcctgtctt ttgaagccat
     1261 tcaaaccttc tcaacaccac gtcgtttggc tgttcgtgtg actggtttat cagacaaaca
     1321 gtctgatttg acagaagatt tcaagggacc agcaaagaaa attgccttgg atagtgatgg
     1381 aaacttcacc aaagcagctc aaggatttgt ccgtgggaaa ggtttgactg ttgaagatat
     1441 cgaattccgt gaaatcaagg gtgaagaata tgtctatgtc actaaggaag aagttggtca
     1501 agcagttgaa gccattgttc ctggtgttgt agatgtcttg aagtcattga ctttccctgt
     1561 cagcatgcac tgggctggaa atagctttga atacatccgc cctgttcaca ctttaactgt
     1621 tctcttggat gagcaagagt ttgacttgga tttccttgat atcaagggaa gtcgtgtgag
     1681 ccgtggccat cgtttcttgg ggaaagaaac caaaattcag tcagcattga gctatgaaga
     1741 agaccttcgt aagcagtttg taatcgcaga tccatgtgaa cgtgagcaaa tgattgttga
     1801 ccaaatcaag gaaattgagg caaaacatgg tgtacgtatc gaaattgatg cggatttgct
     1861 gaatgaagtc ttgaatttgg ttgaataccc aactgccttc atgggaagtt ttgatgctaa
     1921 ataccttgaa gttccagaag aagtcttggt gacttctatg aaggaacacc agcgttactt
     1981 tgttgttcgt gatcaagatg gaaaactctt gccaaacttc atttctgttc gtaacggaaa
     2041 cgcagagcgt ttgaaaaatg tcatcaaagg aaatgaaaaa gtcttggtag cccgcttgga
     2101 agacggagaa ttcttctggc gtgaagacca aaaattggtg atttcagatc ttgttgaaaa
     2161 attaaacaat gtcaccttcc atgagaagat tggttccctt cgtgaacaca tgattcgtac
     2221 gggtcaaatc actgtacttt tggcagaaaa agctggtttg tcagtggatg aaacagttga
     2281 ccttgctcgt gcagcagcca tttacaagtt tgacttgttg acaggtatgg ttggtgaatt
     2341 tgacgaactc caaggaatta tgggtgaaaa atacaccctt cttgctggtg aaactccagc
     2401 ggtggcagct gctattcgtg aacactacat gcctacatca gctgaaggag aacttccaga
     2461 gagcaaggtc ggcgcagttc tagccattgc agacaaattg gatacgattt tgagtttctt
     2521 ctcagtagga ttgattccat caggttctaa tgacccttat gcccttcgtc gtgcaactca
     2581 aggtgtggtt cgtatcttgg atgcctttgg ttggcacatt gctatggatg agctgattga
     2641 tagcctttat gcattgaaat ttgacagttt gacttatgaa aataaagcag aggttatgga
     2701 ctttatcaag gctcgtgttg ataagatgat gggctctact ccaaaagata tcaaagaagc
     2761 agttcttgca ggttcaaact ttgttgtggc agatatgttg gaagcagcaa gtgctctcgt
     2821 agaagtaagc aaggaagaag attttaaacc atctgttgaa tcactttctc gtgcctttaa
     2881 cctggctgag aaggcagaag gggttgctac agttgattca gcactatttg agaatgacca
     2941 agaaaaagct ttggcagaag cagtagaaac actcgtttta tcaggacctg caagtcagca
     3001 attgaaacaa ctctttgcgc ttagcccagt cattgatgct ttctttgaaa atactatggt
     3061 aatggctgaa gatcaggctg tccgtcaaaa tcgtttggca atcttgtcac aactaaccaa
     3121 gaaagcagct aagtttgctt gttttaacca aattaacact aaataaaatt tgataaacgg
     3181 actttatctt attacaaagg agaagaaatg gatccgaaaa aaattgctcg tatcaatgag
     3241 cttgctaaaa agaaaaaaac agaaggctta acaccagaag aaaaagtgga acaagccaaa
     3301 ctacgtgagg agtacatcga aggttatcgc cgcgctgttc gtcaccacat tgaaggaatc
     3361 aaaattgtgg acgaagaagg aaacgatgtt acaccagaaa aactacgcca agtacaacgt
     3421 gaaaaaggat tacatggccg tagtcttgat gacccaaatt cataataata ctcttcgaaa
     3481 atcaaattca aaccacgtca gcttcacctt gccgtactta agtacagcct gcggctagct
     3541 tcctagtttg ctctttgatt ttcattgagt ataagtactc tttcttttaa caaagataga
     3601 tgaaacgata acaaagagac tagcagtttg tgtttgctag tcttttttcg ctaaaaaagg
     3661 aaccataatg gttcctaaaa actatcatta gtaacttgca ccggctgtag cgtctgcgtc
     3721 accaccgtgg cccccagcat cccctgaatc agaagcgcca gaagtagcat cggcgtctcc
     3781 atgacctccg gcagcaggag caaatggtcc gctaccaccc accaaacgtt gaccagtctc
     3841 ttttaggtac cagtcaagcc atggttggaa gttaaagacg atttcattga taccagcgta
     3901 tgatccatca ggatagtaca ttgcttggta gttgtgagtg ttgataacac ctgcaggaga
     3961 acctggaacg atcgtacgga cgtattcttg gtttccgttg cgaagtgttc cgataaccca
     4021 ctctacgttc ttcatacgtg ctggtggaag agaaccatga acagtcgaca tacggctacc
     4081 tgattgaggt ggtacacgtt tagcgaacat agtgtctgga tcttggtgag cgttgttgta
     4141 gtagaggaat tggttgttgt cgtcagcgta tgtcaattca aatggcatag ctttcaagaa
     4201 catatcaatt tggttaactg ttaggatacc gtggtccaat ttgacatagg tatcaccaga
     4261 aacagcacca gtgattgctg caactttttc tacccattct ggatcgtcag ggtcaacttc
     4321 tgtgatggtt gtagcgattg gttttccaca atccaagtct tctgattcga ttggttttgg
     4381 ttttttcaat ttcgaaacga ctcctacgta tttaacaaag ttatctaagc aagtttcaag
     4441 gaatttaaca gtgccttcgt tggtgatatt tccgttgtta tcaaaagctt ccttagcttt
     4501 accaagaagg aattcgttac ctggaagcgt gtaggcatta acacctggag catcaaggat
     4561 tttacgaagg tgaacttgag cacgtgatgt tccttggtca tagtatgatg cacccacaat
     4621 cataacaggc ttgttttcaa atggatgaac ttcgtatgaa agccattcaa gtacagattt
     4681 gagtgaagct gagatagtgt ggttatgctc aggagtagca atgataacac catctgcacg
     4741 agtaattttg ttatataaat aacgtaattg gaaactttca tcccattttt catcttggtt
     4801 aaacattgga acttcgtcaa tttcaagaac ttctaattca aatttgagtt tgaagtagcg
     4861 acggataaat tccaagagct tacggttata tgattgatcg tagtttgatc caacaagtcc
     4921 aacaaatttc attctttttg gtctcctatc ttacaaattt tcccagtcaa agtcttcagc
     4981 atctttgcga agtaattctt gtgcattacg taatttttct gtgattttta caaagatacg
     5041 gaagtcatca aagatggcat ccaatttctt gataacatca aggtcaacca agtcgccact
     5101 tgggttaaat gcttgaagag agtgtgagag caagaattca tctggaagaa catttgcctt
     5161 gatttcagga gcattcaaga tttgacgaag ttgcaattgg gcacgagatg aaccaagcgt
     5221 accgtaagaa gcacctgtaa tcatgattgg tttgttcaaa agtgggtaaa taccataaga
     5281 caaccaagca agagcgctca tcaaaacagc tggaatagag tgatcatact caggagtacc
     5341 gataataacg ccatctgcct cttcgatttt agcagcaatt tccaatattt cagcaggtac
     5401 ttgcttgtca gctggtttgt tgaagacagg aatggccttg atttcaacaa gctcaatttc
     5461 agctttgtca gaaaagtgtt tttgcatgta ttgaagcaat tgacggtttg tagaacgttt
     5521 tgaatttgtt ccaacaatag caataagttt taacatgaga tttcctttct ctttttacat
     5581 aatacaattt taaaattcca ttgaaacagt tgtctctata gagtaggaat tcctgaagaa
     5641 cagcttaggt ggccttcttt atcgatgagg atgacttcga tgccctccaa actttcgact
     5701 tgccagagga tagaagcagg tctttctcca aagagtcgag tcgtccagat ttcgccatcg
     5761 actgatttat cagagatgat tgttagactc gctagttccg tttcaacagg atatcctgtt
     5821 tgactgtcaa aaatgtgatg gtaatcttgt ccatcgacgg tcaggtgacg ttcataaatg
     5881 cctgaagtca cgacagattt attgacaaca gggatggtca ttaaatgatt tcccctagga
     5941 ttggctgggt cttgaatccc gatttgccat gggttatccc ctcttgcctg attttttcca
     6001 atggtcagga tattccctcc cagattgatc aaggcagaag tcaccccctc tttcctaaga
     6061 aattgggcaa ccttatccgc actgtatcct ttggctaaac aacctagatc gatcttcatt
     6121 cctttctgtt ttaaaaacac agtagaagta gaagaatcta actcgatacc atgaggattg
     6181 attagaggca gcaccgattc aatttcttga ggctgggcga ccttggcatc tgaaaaaccg
     6241 atacgccagg tttgaattaa gggaccaatg ctgatattga ggtggctaga gagcgctagg
     6301 ctatgctcta acccaagtga aatcagctca aacaggtctg gatgaaccgt gacgggggct
     6361 attcctgctt gataattgat ttccatcaac tcagattctt gactattggc gttgaagcgg
     6421 tattcaagtt ctttgagcaa gtcaaaggat ttttggagaa agatatcggc ttgctcatcc
     6481 actaatgaaa tagtgatagt agtccccatt agccgttcag aatgtgaacg aagagtcaag
     6541 ctaccaactc ctttctctta tagaaaataa gttgtaatat caaataatca tctaaattga
     6601 agcccttaca tttcattttc at
//
LOCUS       DAVMGZ010000100         6613 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000100 DAVMGZ010000000
VERSION     DAVMGZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.101"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            72..467
                     /locus_tag="VYQ93_001610"
     CDS             72..467
                     /locus_tag="VYQ93_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV3336268.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            478..849
                     /locus_tag="VYQ93_001611"
     CDS             478..849
                     /locus_tag="VYQ93_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV3336269.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            859..3102
                     /locus_tag="VYQ93_001612"
     CDS             859..3102
                     /locus_tag="VYQ93_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV3336270.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            3309..5084
                     /gene="spxB"
                     /locus_tag="VYQ93_001613"
     CDS             3309..5084
                     /gene="spxB"
                     /locus_tag="VYQ93_001613"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV3336271.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            5195..5542
                     /locus_tag="VYQ93_001614"
     CDS             5195..5542
                     /locus_tag="VYQ93_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV3336272.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(5659..6570)
                     /locus_tag="VYQ93_001615"
                     /pseudo
     CDS             complement(5659..6570)
                     /locus_tag="VYQ93_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1948 a         1262 c         1480 g         1923 t
ORIGIN      
        1 attgacaaat gtagattttg ggagtatact gttgagtgta attgacaaat gtagattttg
       61 gaggtatggt aatgcagatt tcagatgcag aatggcaggt catgaagatt atttggatgc
      121 agggggagca gaccagtaca gatttgatta gggttttggc agagcggttt gactggtcca
      181 agtccacggt tcagacactt ttggctcgtc tagttgagaa agagtgtttg actcggaaaa
      241 aagaaggcaa gtcctttgtt tattcagcgc ttttaactct agaccaaagt cgggatttac
      301 ttgtccaaga tatcaaagac aaggtttgtt cccgtaggat taggaatttg ttggctgact
      361 tgattgtaga atgtgaattt actcagactg acttggaaga cttggaagct gtgatttcag
      421 agaagaaatc aagtgctgta acagaagtaa gatgtaattg tatgtaaagg agacgtcatg
      481 ttaaatagta ttgtaaccat tatttgtatt gcccttatcg cgtttatctt gttttggttt
      541 ttcaaaaagc ctgaaaaatc tggacaaaaa gcccagcaaa aaaacggata ccaagagatt
      601 cgagtggaag tcatgggagg ctatactcct gagttgattg tcctcaagaa atcagtgcca
      661 gcccgcattg tctttgaccg caaggatcct tcaccatgtc tggatcaaat tgtttttcca
      721 gattttggtg tacatgcgaa cctgccaatg ggggaagagt atgtagtgga aatcacgcct
      781 gaacaggctg gagagtttag ctttgcttgt ggtatgaaca tgatgcacgg caagatgatt
      841 gtagagtagg tggagactat gacagaaatt gtgaaagcaa gcttagaaaa tggcattcaa
      901 aaaatccgta tccaagctga aaaaggctat catccagccc atatccagct tcaaaaggga
      961 attccagctg agattacctt tcatcgtgct actccttcaa actgttataa ggaaattctg
     1021 tttgaagaag aaggtatctt ggaaccaatc ggcgtagatg aggagaaagt cattcgtttt
     1081 acacctcaag aattagggcg acatgaattt tcttgtggca tgaagatgca aaagggaagc
     1141 tatacagtcg ttgagaagac tcgaaaatct ctatctctcc tgcaacgttt ttggattact
     1201 agtatctgta ctgtgcctct tgtgattctc atgattggga tgttggcagg tagcattagt
     1261 catcaagtca tgcattgggg aacctttcta gcaacaacgc ctattatgtt agttgcaggt
     1321 aagccatata tccagagtgc ttgggccagt tttaaaaagc acaatgccaa catggatacc
     1381 ttggttgcgc tgggaactct agtggcttat ttctatagcc tagttgctct ctttgctggt
     1441 ctccctgttt acttcgaaag tgctggattt atcctctttt tcgttctttt gggagcagtt
     1501 tttgaggaaa aaatgagaaa aaatacgtcc caagctgtgg agaaattact ggacttgcaa
     1561 gctaaaaccg cagaagtctt gagtgatgat agttatgtcc aagttccttt ggaacaagtc
     1621 aaggtaggcg acctgattcg agtgcgtccc ggtgaaaaga ttgctgttga tggtgtcgta
     1681 gtagaaggtg tctctagtat tgacgaatcc atggtgacag gtgagagtct gcctgtggac
     1741 aagacagttg gagatactgt cattggctca accatcaatc atagtggaac gcttgtcttt
     1801 agagcagaaa aagttggctc agagactgtt ttggctcaga ttgtggattt tgtgaagaaa
     1861 gctcagacaa gtcgtgcgcc gattcaggac ttgacggata agatttcagg gatttttgtc
     1921 ccagtagttg tcattttagg aatcatgacc ttttgggttt ggttcgtctt gctcagggat
     1981 agtgtggttg tgcttggagc tagctttgtg tcctctcttc tctacggagt ggcggttttg
     2041 attatcgcct gtccttgtgc cttgggactt gcaacaccga cagcccttat ggtggggaca
     2101 ggacgtagtg ccaagatggg ggttctcctc aaaaatggaa ctgtcttaca ggaaatccag
     2161 aaagttcaaa cccttgtctt tgataagacc gggactttga cggaagggaa acctgtggta
     2221 acagatgtca tcggcgacga agtagaagtg tttggattgg cagcctcctt ggaagatgct
     2281 tctcaacacc cactggctga ggctatcgtt aagcgagcga gtgaagctgg acttgagttt
     2341 caaactgttg aaaattttca ggccttgcac gggaaaggtg tttcagggcg aatcaatgga
     2401 aaacaagttt tacttggaaa tgctaaaatg ctggatggca tggatatttc taatacttat
     2461 caagataaac tagaagaact agaaaaagaa gctaagacag ttgtgttctt agctgttgac
     2521 aatgaaatca aaggcttgct tgctttgcaa gatattccta agaaaaatgc taagctagcc
     2581 atcagtcagc taaaaaaacg tggtctcggg acagtcatgc tgacaggaga caatgctggt
     2641 gtggcgcgtg ctattgcaga tcaaatcgga attgaagagg tcattgcagg cgtcttgcca
     2701 gaagaaaaag cccatgaaat ccataaactg caacagtcag gtaaagtagc ctttgttggt
     2761 gatgggatca atgacgctcc tgcccttagt gtagcagatg tggggattgc tatgggagct
     2821 ggaacagata tcgccatcga gtcagcagat ttggtgttga caaccaataa tcttttagga
     2881 gtggtgcgtg ccttcgacat gagtaagaaa acctttcatc gtattctgct caatcttttc
     2941 tgggctttta tctacaatgt tgtcggaatt ccgattgcag caggagtctt ttcaggtgtt
     3001 ggactggctc tcaacccaga attggcaggt ctagccatgg cctttagttc tgtatctgtt
     3061 ctgaccagtt cccttctgtt aaattttagt aaaatagact aaaagtaatg actagatttt
     3121 tttgttataa aacagaaatg acgacacttt caaaactgta ttagaataga gaatagagag
     3181 ttttgagcag atttttagaa aagtcagcat aaatatgata cagtggaata gtaaaaattt
     3241 ggagaacgtt tccaattcta tgtaatcgta ttctccaagt ctaaaaaaat tgaaggagag
     3301 ttatcattat gactcaaggg aaaattactg catctgcagc aatgcttaac gtattgaaaa
     3361 catggggcgt agatacaatc tacggtatcc catcaggaac actcagctca ttgatggacg
     3421 ctttggctga agacaaagat atccgcttct tacaagttcg ccacgaagag acaggtgctc
     3481 ttgcagcggt tatgcaagct aaattcggcg gctcaatcgg ggttgcagtt ggttcaggtg
     3541 gtccaggtgc gactcacttg attaacggtg tttacgatgc agctatggat aacactccat
     3601 tcctagcaat ccttggatca cgtccagtta acgaattgaa catggatgct ttccaagagc
     3661 ttaaccaaaa cccaatgtac aacggtatcg ctgtttacaa caaacgtgta gcttacgctg
     3721 agcaattgcc aaaagtaatt gacgaagcct gccgtgctgc agtttctaaa aaaggtccag
     3781 ctgttgttga aattccagta aacttcggtt tccaagaaat cgacgaaaac tcatactacg
     3841 gttcaggttc atacgaacgc tcattcatcg ctcctgcttt gaacgaagtt gaaatcgaca
     3901 aagctgttga aatcttgaac aatgctgaac gcccagttat ctatgctgga tttggtggtg
     3961 ttaaagctgg tgaagtgatt actgaattgt cacgtaaaat caaagcacca atcatcacaa
     4021 ctggtaaaaa ctttgaagct ttcgaatgga actatgaagg tttgacaggt tctgcttatc
     4081 gtgttggttg gaaaccagcc aacgaagtgg tctttgaagc agacacagtt cttttccttg
     4141 gttcaaactt cccatttgct gaagtttacg aagcattcaa gaacactgaa aaattcatcc
     4201 aagttgatat cgacccttac aaacttggta aacgtcatgc ccttgacgct tcaatccttg
     4261 gtgatgctgg tcaagcagct aaagctatcc ttgacaaagt aaacccagtt gaatcaactc
     4321 catggtggcg tgcaaacgtt aagaacaacc aaaactggcg tgattacatg aacaaactcg
     4381 aaggtaaaac tgagggtgaa ttgcaattgt atcaagttta caatgcaatc aacaaacatg
     4441 ctgatcaaga cgctatctac tcaatcgacg taggtaacac tactcaaaca tctactcgtc
     4501 accttcacat gacacctaag aacatgtggc gtacatctcc actctttgcg acaatgggta
     4561 ttgcccttcc tggtggtatc gctgctaaga aagacaatcc agatcgccaa gtatggaaca
     4621 tcatgggtga cggagcattc aacatgtgct acccagacgt tatcacaaac gttcaatacg
     4681 accttccagt tatcaacctt gtcttctcaa atgctgagta cggcttcatc aagaacaaat
     4741 acgaagatac aaacaaacac ttgtttggtg tagacttcac aaacgctgac tacgctaaaa
     4801 ttgcggaagc tcaaggagct gttggattca cagttgaccg tatcgaagac atcgatgcag
     4861 ttgttgcaga agctgttaaa ttgaacaaag aaggtaaaac tgttgtcatc gatgctcgca
     4921 tcactcaaca ccgtccactt ccagtagaag tacttgaatt ggatccaaaa cttcactcag
     4981 aagaagctat caaagccttc aaggaaaaat acgaagcaga agaactcgta ccattccgtc
     5041 tcttcttgga agaagaagga ttgcaatcac gcgcaattaa ataattcctc tcgccgaaaa
     5101 tcaaatatga aacttgtaga ctttattctt tgattttcat agagcagaac tttaaaagat
     5161 cggagcaatc cggtcttttt atggaggaga gtaaatgaat ttaaatcaat tagatattat
     5221 cgtttcaaat gttccccaag tctgtgctga cttggagcat attttggata aaaaggcaga
     5281 ttatgctgat gatggttttg ctcagtttac gattggtagt cactgcctta tgttgtcaca
     5341 aaatcatttg gttcctttgg aaaactttca gtcaggaatc attattcata tcgaggttga
     5401 ggatgtagac cagaactaca aacggttgaa cgagcttggt ataaaggttt tacacggacc
     5461 aactgtaacc gattggggaa cagagtcctt attagttcaa ggacctgctg gtctagtgct
     5521 tgatttttat cgtatgaaat aggtcgcgta gtcatttgac gttaacctta gctattttta
     5581 aaatagaaat tgagaatatg aactattgaa agatcggagc aatccggtct ttttatataa
     5641 agaaattgca tataatactc atatgctttc aaaaagcatt ctagtccatc tccgattaac
     5701 gatggacttt atcacctcct tctccagtcc ttgtatgaca tcttgaagtt gattcatgac
     5761 atcttccaaa gttcgaaagg ccttattctt aaatccacgt ttacgaatct ctttccacac
     5821 ttgttcaatg ggttcatctc tggtgtgtat ggaggaataa aggtaaaacc aatattagtc
     5881 ggaatcttta aggtacttga tttatgccat atagcattgt ccataacgag taaaagataa
     5941 tcatctggat aagcttgtga aagctcttct aaaaaggcgt tcatccactc agtattacat
     6001 ccaccagcta ttaagaaaaa tgattcgcct gtatgggcat caacagctcc ataacaatag
     6061 tgaaattctc gtatatagtg actatggaca tgtggaccta ctcctattgg agcccaacaa
     6121 gatcccagtt tactgattct accgaaacca gcctcatctt ggtacatcaa gcgaacctta
     6181 cgaaaacgtc tactgctttt aaagcgcttt cttttcttct tgaattgaga ttttattttt
     6241 agacgcgaca atggtttgag cgtctgcttt cctaggatgt tctggacgtg gcataatatt
     6301 tcgccaacca tggtgcttca acagttgata gaaggcatca cgtgtgtagg aacgacctaa
     6361 ctccttttta taagcctgaa ataaggcatc aattgtaaca aattctcctg cctctgtagc
     6421 cttcaaatgg cgggcaagaa agactttctt ttcctcaatc gtcatatatg catggttacg
     6481 gccaccacgt gtttcttgaa ggagagagtc gagttcgaac tcctcatatt tttttacatt
     6541 tcgccaaatc gttgtttgat tacagtctat agtgtattga atctataaca gtacacctta
     6601 gctgctaaaa tat
//
LOCUS       DAVMGZ010000101         6588 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000101 DAVMGZ010000000
VERSION     DAVMGZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.102"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     regulatory      82..180
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            264..1055
                     /gene="thiD"
                     /locus_tag="VYQ93_001616"
     CDS             264..1055
                     /gene="thiD"
                     /locus_tag="VYQ93_001616"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV3336273.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(1265..1897)
                     /locus_tag="VYQ93_001617"
     CDS             complement(1265..1897)
                     /locus_tag="VYQ93_001617"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV3336274.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(1890..2693)
                     /locus_tag="VYQ93_001618"
     CDS             complement(1890..2693)
                     /locus_tag="VYQ93_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV3336275.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(2693..3217)
                     /gene="thiW"
                     /locus_tag="VYQ93_001619"
     CDS             complement(2693..3217)
                     /gene="thiW"
                     /locus_tag="VYQ93_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV3336276.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(3222..3914)
                     /gene="tenA"
                     /locus_tag="VYQ93_001620"
     CDS             complement(3222..3914)
                     /gene="tenA"
                     /locus_tag="VYQ93_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV3336277.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(3925..4575)
                     /locus_tag="VYQ93_001621"
     CDS             complement(3925..4575)
                     /locus_tag="VYQ93_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV3336278.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(4577..5962)
                     /locus_tag="VYQ93_001622"
     CDS             complement(4577..5962)
                     /locus_tag="VYQ93_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336279.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLNGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(5963..6523)
                     /locus_tag="VYQ93_001623"
     CDS             complement(5963..6523)
                     /locus_tag="VYQ93_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3336280.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         2042 a         1437 c         1306 g         1803 t
ORIGIN      
        1 attttaatca tttgaaaaac gatttgcagt caaatattac tatataaaca ataaaaatat
       61 gctatactaa agaaaaaaga aaacaaccac taggggtgcg taaagctgag attaacgact
      121 gttagatccc tctgactcaa tctaggtaat gctagctgat ggaagtggaa atgataatgg
      181 ggactagcag tcttctattg cctttctaaa acagactagc ttgttcttaa gaatacaaac
      241 ttcagttggt tgggaggttt tagatgactt atttacccgt tgctttgacc attgcaggga
      301 ctgaccctag tggtggtgct ggcattatgg cagatttaaa gtcattccaa gcgagagatg
      361 tctatggaat ggctgttgta accagtcttg tcgctcaaaa taccagaggt gttcagctaa
      421 tcgagcacgt ttctcctcaa atgttgaaag cccaattgga gagtgtcttt tctgatattc
      481 cacctcaggc tgtaaaaact ggaatgttgg ctactactga aatcatggaa atcatccaac
      541 cctatcttaa aaaactggat tgtccctatg tccttgatcc tgttatggtt gctacaagtg
      601 gagatgcctt gattgactca agtgctagag actatctcaa aacaaactta ctacctctag
      661 caactattat tacgccaaat cttcctgaag cagaagagat tgttggtttt tcaatccatg
      721 accccgaaga catgcagcgt gctggtcgcc tgattttaaa agaatttggt cctcagtctg
      781 tggttatcaa aggcggacat ctcaaaggtg gtgctaaaga tttcctcttt accaagaatg
      841 aacaatttgt ctgggaaagc ccacgaattc aaacctgtca cacccatggt actggatgta
      901 cctttgctgc agtgattact gctgaactag ccaagggcaa gagtctttac caggcagttg
      961 ataaggccaa ggcctttatc acaaaagcta ttcaagatgc ccctcaactc ggtcatggtt
     1021 ctggtccagt caaccataca acttttaaag attaagaaaa aaaactctct agttcccact
     1081 ttaagggaat tagagagttt ttatactctt cgaaaatctc ttcaaactac gtcagcttcc
     1141 atctgcaact tcaaaacact gttttgagct gacttcgtca gtcttatcta aaacctcaag
     1201 gcagtacttt gagcaacctg cgactagctt tctagtttac tctttgattt tcattgagta
     1261 ttaattagga aagaatgtca tgcaactttg ttaaaaaggc ttgcgttttt gcctcaatat
     1321 cttctgcttg catcaaatca cgtacaacag ctacaccagc tatgccagtg cccataagct
     1381 gatcaatatt ctccgaagtc aagccaccaa tagcaactat tggaatggca accgtttggc
     1441 aaattgtttt caaggttgaa atcagggtga tgggtgcatt ttccttggtg gttgtcggaa
     1501 aaatggctcc tgtccccaag taatctgcac ctgatttctc cgcttccaga gctcttttaa
     1561 ccgttttagc ggtgacaccg aggatttttt caggacccaa gacttgtcgg gcaaccgaaa
     1621 ctgggagttc gtcatctcca atatgcagac cagcagcatc aaccgcaaga caaacatcca
     1681 accgatcatc gattatcaag ggtacctgat aagcatctgt tatttccttg acttgttttg
     1741 ccagttgata atattgattg gttgtgagat ttttttctcg caattggact atggtaaccc
     1801 ctgaacggca ggccgtctca acttttgcaa gaaagctttc cacggaatct tgatagcgat
     1861 tggttaccag atatagtcta agtgcttctc tattcataaa cctctccttt gatggtatct
     1921 agccaatttt catctcttct taggagcgaa agctgattga gtacttggta acgaaattct
     1981 tccaatccca ttccttgaac aactattttc tcagcagcga tattgagata agagactgct
     2041 aagcaagaag cttcaaaacc agtctttcct tggctgagaa aaacggctgt taaggctcca
     2101 accaaatctc ctgtccctgt tatccagtct aattctgcac agccatttcc cagtacagcg
     2161 acctgatttt tcgaaacgac gaggtccttg ggacctgtga ctaagaaaga cataccatga
     2221 taggtctgac accagtcttt caagacttga agcaaatcct ccgtttcttg atctttagca
     2281 ctcgcatcga ccccaacgcc gtggtgcttt aatccaacaa gacttcgaat ttctgacatg
     2341 tttcctttaa ggaccgtagg tctatagtct aaaaggtctc tgactaagct cttacgaatg
     2401 gatgaagctg ttacgccaac cgcatctact accattggga gacaaacttg agctgcataa
     2461 gaagctgcta tacggattgc tttctccttc tcagctgaca aatgccccaa attgatgaag
     2521 agagcctgac tttgcttagt aaaatcaaga acctcacggg gatcatctgc catgacaggt
     2581 ttgcatccca gagccaaaat cccatttgcc agcatctcac aagaaatctc attggtcata
     2641 cagtgaatga gggaactaga gcctatagga aagggatttg taaattcctg catcagtcta
     2701 tcctttcact aaagaaatat ccctgcactt ttttaaagaa ttcctgcttg attaaaaatc
     2761 gaaaggcaat aaaggaaatc gctgtaccaa tcaaggttgc tccgaaaaat cgaggcgtgt
     2821 agataaacca gctaagctta gcagctgatc ctgtaaagag taccataaca ggataggaaa
     2881 caatagaacc aataatacct gttcccacaa tttctcccaa ggcagaaaag taaaattttc
     2941 gaccgtactt ataaaagaga cctgctagaa gggctccaaa agtcgctcct gtgagagcta
     3001 aaggcggaat cccttgagtc gtcatacgga taaaggctgt cactgtagcc atagccaagg
     3061 cataaacagg tcccatcatg attcccgcta gaatattgac tacactggac atcggtgcca
     3121 ttccctcaat ccgaaagata ggtgtaagga ctacatcaag ggcaatcatc atagataaaa
     3181 tggtcaattt gtgaacttgt agttggtgct ttctcatgtt tctattcttc tcctttttct
     3241 aaagactgta aatcgctctt ccatgtctgg tgttggtaag ccatctccca aaacttggct
     3301 tccatatgaa cactgatgtg gaaggcatct agcatttttt gcttatctgt ctcatcactt
     3361 tctcgataga gctgattgac cagtgctccc tcctctctga tctgttgctc taactcatcc
     3421 gtaatataag tttcaatcca ttgttgatag agaggatttg gtgatggttt aagattaagt
     3481 gatttgccta tatcatggta taaccaagga caaggaagca agcttgcaaa agcgatggct
     3541 aagttcggtt ctgcaaattg cctataaata tgagaaatgt aatgataaca ggttggagcg
     3601 attggatgtt gctccatttc ctggtcgctg atttccaatt ccttgaaaaa ttgttggcga
     3661 ataaataact caccctccac taaaccctga gcattttgtt tcaagagtct tttcatctct
     3721 tggtttgaag tcttatcagc caaaagatga tagatttctg agaaagcctt cagatagtag
     3781 gcatcctgaa tcaggtaata gcggaaaatg gcaggttcta aattcccctc ttgtaattgt
     3841 aaaataaagg gatgatgaaa ggaagcctgc caagctttct tggataattc catcgcaata
     3901 tctgtaaatt ccataataac tcctttataa aaatagactg gtttgaagca ataaaaagaa
     3961 aagcaggtag attaattttg tttttttagg aatataaaaa gtccgatagc tattcttcaa
     4021 ctgtgcatgt tcgtcatatc cgtgagcaga tagagctctc aggtaaagat ggcgccacct
     4081 aaagactgtc atcagaacct tactgtaaat caagggcgac caaaaatgta gttcttgacc
     4141 acgtaatagg caagcttctt tgagggactt gatttcttgc tgaatgagag gaaaagaatt
     4201 gaataccaca atcaaggcat aggaccaaga gcgtgatagc cccttttgag ccaagtacaa
     4261 gagaagctct tttagtgaaa tagaggaaac aaagacaagg ccgatacaaa ctgtcacaaa
     4321 ggccctcgtt ccaagcatga ctgcctgtga agcatctccg tgtaactgaa ctgcccagta
     4381 gttggcaaaa gatggtaaaa tggcaagtat gatcatccaa gctaacattt taaatcgacg
     4441 gtaatagagc ataaagagaa tacaaaatgc gactaccgaa agattcagag caatcgaagg
     4501 aatgaaagat gtttccaagg ataaaatcag caagaagaga ctgataatcg gtgtctgggt
     4561 tgctactttg accatactat ctcacctccc cttgggtatt gctactctga gatgtaagtg
     4621 gtttggtaat ggtcacttct ttcacatgcc gaagaccctg actagtcatc tcaatccaat
     4681 aatcaaccac agaaatcaaa gggtctaaac gatgactaat gagcagaaaa cttcttcctt
     4741 gattcctctc ctccacaatc cacttgcaaa aataatggca ggctctatca tccaaacctg
     4801 caaaaggttc atctagcaag atcacggaag ccttactggt caagatggtc aggagctgaa
     4861 gaattttttg ctgaccacca tttaattgat agggactctt atcgactgcc tgctccaaat
     4921 caaaatatcg taaagcttga aaaatccgct gatttctttc agaatcaggt ccatctaatt
     4981 gaagctcctc tcgcagactg actcggataa actgcttctc agcttcctga acaacaccag
     5041 tcagatcacg atacaaactc tttttctttt tcaggaccga acccttccat gcaatgcgcc
     5101 ccttatactt ttgaaattga agaatagacc gaaagagggt tgatttcccg acaccattgt
     5161 cacccaggat acaggaaatc ccttgataga atgtgaaatc agcaattgaa aagagggggc
     5221 gatttccaaa ctcacaagtc gttctatcca tatggaatag ttccgggcta gaagcaactt
     5281 cctttgaagc aacctgtgtc atctcatagg aagggatttg aaacacttcc cttagttttc
     5341 cgtctcttag ctcaaccata tggtcgatat aggctttata gtcagataaa tcatggtcgc
     5401 acaaaataac tgtctttcca tcaagagcca actcttttag aatctccaat atctcgattc
     5461 tgctcttgcg gtcaatggaa gcaaaaggtt catccaagag atagacccta ggattcatgg
     5521 caaagaggac agccagcgct gctttttgct tttccccacc tgataggtga tgaatgggac
     5581 ggtgtaagat tgcctcgcaa cgacattgct ggacaacctc tgctatttta gaatcaattt
     5641 cctgaaggtg atagccgata ttctccaagg tgaaaatcag ctcctcaaac aagctctcca
     5701 tggtaaattg atgattggga ttttgaaaga gaatgccaac cttctggaca cgtgcgatga
     5761 tagaaagttg actgacctcg ctcccatcta tcaggacttg accactatag ggaagagaac
     5821 tgacttgggc aatcatttga aagaggctgg attttccaga cccactactc ccaactaaca
     5881 aggtaaaggc ttgcgcatga aaagtaaaat caaacggctc agagaagatt ggggactgaa
     5941 tcgctcgtag ttccagaccc atctatgctt ttcctccagt tgcaaactga tgatagagtt
     6001 tgacaatggc acgaaccaag atggtacaga agaaataaac agaaataaaa cgtaccacaa
     6061 gtaaggaaag gacaaacgga agggaaaagg cgtagtaacc taacttaatg tattcataga
     6121 caaagctaac aagcgtaatc ccaatactat tagcagttag agagagccaa ctttcatagc
     6181 gattcttagt tacgataaaa ccaaattcac ttcccaaacc ttgaaccaag ccagacaaaa
     6241 gagctcctag accaaattgg ctaccataaa ggacttcagc aagcgcagct agcacttctc
     6301 caatcgttgc acttccgact ctcggaacaa agatggcagc aatgggcgca gccatacacc
     6361 agagaccgaa gaggatttca ttggcaaagg cctgcaaacc aagaggtgtt aagagtagac
     6421 tgagaatatt atacacatat cctgaaccaa cgaaaacccc accaaaaaag atagacaaga
     6481 aagcaagcaa gataacatct tttaactgcc attttttcaa cataaaaaac tccttttttt
     6541 aaagaaaagt ggggcactca agaagaccga cctaaatact ttgtatag
//
LOCUS       DAVMGZ010000102         6548 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000102 DAVMGZ010000000
VERSION     DAVMGZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.103"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            222..458
                     /locus_tag="VYQ93_001624"
     CDS             222..458
                     /locus_tag="VYQ93_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV3336281.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCHRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            689..1975
                     /locus_tag="VYQ93_001625"
     CDS             689..1975
                     /locus_tag="VYQ93_001625"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV3336282.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            2176..2643
                     /locus_tag="VYQ93_001626"
     CDS             2176..2643
                     /locus_tag="VYQ93_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV3336283.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            2676..2769
                     /gene="ffs"
                     /locus_tag="VYQ93_001627"
     ncRNA           2676..2769
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYQ93_001627"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2852..3993)
                     /locus_tag="VYQ93_001628"
                     /pseudo
     CDS             complement(2852..3993)
                     /locus_tag="VYQ93_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(4048..5118)
                     /locus_tag="VYQ93_001629"
     CDS             complement(4048..5118)
                     /locus_tag="VYQ93_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEV3336284.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            complement(5135..5515)
                     /locus_tag="VYQ93_001630"
     CDS             complement(5135..5515)
                     /locus_tag="VYQ93_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV3336285.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHNSGFYNPDTNVLAIDHNLTPE
                     QQIQVALHELGHKDHTRSEHQNARLRCENEADRNMIHHLVKDALENLDDPTEFDYLKF
                     MSYYNLKTMTNEIMVKEEYLALVN"
     gene            complement(5528..5791)
                     /locus_tag="VYQ93_001631"
     CDS             complement(5528..5791)
                     /locus_tag="VYQ93_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV3336286.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            complement(5791..6024)
                     /locus_tag="VYQ93_001632"
     CDS             complement(5791..6024)
                     /locus_tag="VYQ93_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336287.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            complement(6024..6392)
                     /locus_tag="VYQ93_001633"
     CDS             complement(6024..6392)
                     /locus_tag="VYQ93_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3336288.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
BASE COUNT         1791 a         1011 c         1381 g         2365 t
ORIGIN      
        1 aaaaatatta tagttctaat aggatttacc caaaagtttt aaggcggtct ttttagaact
       61 ttaattgttt gaaatttagg tagcaaattt gtttctattt tgtcaacttt tcctattttt
      121 atcttgttga ggctggtatt ttaacaattc aggaattgat agtgaatgtg taaaattttt
      181 tgttagaata agtttataaa aaagaaaagg agtatttgat tatgttacaa aaaatttatg
      241 agcagatggc taatttctat gatagtattg aagaagagta tggtcctaca tttggtgata
      301 attttgactg ggaacatgtt cattttaaat ttttaattta ttatttagtg agatatggca
      361 ttggttgtca tagggatttt atcgtttacc attatcgtgt tgcttatcgt ttgtatcttg
      421 aaaaattggt aatgaatcgg ggttttattt cttgttgagg taattttagt aaatttccga
      481 actaatttac tcttttatgt aaagataata gtaaatagct agtaattttt ctaaatcatt
      541 ttttaatagt tggaaatagc aaatctttct attgtttctt cttgataaaa aggcgatttt
      601 ttattataat aaattgtaag atataattgc aggtgagagt cctgccatgt atgtgagaaa
      661 ggaagagcct gagggctcag acaaaattat gacttcagtt gttgttgtag gtacccaatg
      721 gggtgatgaa ggtaaaggga agattacaga cttcctttca gcgaatgcag aagtgattgc
      781 acgttaccaa ggtggtgata atgctggtca cacgattgtg attgacggta agaaatttaa
      841 gttgcacttg attccatctg ggattttctt ccctgaaaaa atatctgtca ttgggaatgg
      901 tatggttgta aatcctaaat ctcttgtaaa agagttgagc tatcttcatg aggaaggtgt
      961 aacaactgat aacttgcgta tttctgatcg tgcgcatgtt attttgcctt atcatatcga
     1021 gttggatcgc ttgcaagaag aagctaaggg cgacaataag attggtacga caattaaggg
     1081 aattggtcca gcttatatgg acaaggctgc tcgtgttgga attcgtattg cagatctttt
     1141 agataaagat attttccgtg agcgtttaga acgtaacctt gctgaaaaga atcgtctttt
     1201 tgaaaaattg tatgacagta aagcgattgt tttcgatgat atttttgaag aatattacga
     1261 atatggtcaa caaatcaaga aatacgtgat agatacatct gttatcttga atgatgcgct
     1321 tgataatggc aaacgtgtgc tttttgaagg tgcacaaggt gttatgctag atatcgacca
     1381 aggtacttat ccatttgtta cgtcatcaaa ccctgtagct ggtggtgtga caattggttc
     1441 tggtgtcggt ccaagcaaga ttgacaaggt tgtaggtgta tgtaaagctt atacgagtcg
     1501 tgtaggagat ggtcctttcc caactgagtt gtttgatgaa gtgggagaac gtatccgtga
     1561 agtgggtcat gagtatggta caacaactgg tcgtccacgt cgtgtaggtt ggtttgactc
     1621 agttgtgatg cgtcatagcc gtcgtgtttc tggtattact aacctttctt tgaactctat
     1681 tgatgttttg agcggtttgg atactgtgaa aatctgtgtg gcctatgatc ttgacggtca
     1741 acgtattgac tactatccag ctagtcttga gcaattgaaa cgttgcaagc ctatctatga
     1801 agagttgcca ggttggtcag aagatattac cggagttcgc aatttggaag atcttcctga
     1861 gaatgcgcgt aactatgttc gtcgtgtgag tgaattggtt ggcgttcgta tttctacttt
     1921 ctcagtaggt cctggtcgtg aacaaacaaa tattttagaa agtgtttggt cctaagagat
     1981 ttttaagatt tgtttaagat aggtcgggta tactatagac agttacaaga agacctccta
     2041 acttgttgta acaaatatcc taaacttttc tttttcataa taatctccct atagagtcac
     2101 cgcattcggt ggcttctttt gtgttgggat tcatgatata ataataaaat cgataagtag
     2161 gaaaagagaa aagagatgta ttatacgctt gaagaaaaag aagtctttat gagggaggct
     2221 ttgagagagg ctgagattgc tcttgaacac gatgaaattc caattggttg tgtgattgtc
     2281 aaagatgggg aaatcattgg tcgtgggcat aatgcgcgtg aggaattaca gcgagcggtt
     2341 atgcatgcgg aaattatggc tatagaggat gcgaacttga gtgaggagag ctggcgcttg
     2401 ctggattgca cactttttgt gaccattgaa ccttgtgtca tgtgtagtgg ggcgattggg
     2461 cttgcccgca ttccaaatgt ggtctatggg gctaaaaacc agaaatttgg cgctgctggg
     2521 agtttgtacg atatcttgac agatgagcgt ctcaatcatc gtgtggaggt tgaaatagga
     2581 attttggaag atgaatgcgc agctatcatg caggattttt ttagaaatag acggaaaaaa
     2641 taattttgct tttaaaatga ataggaatgt gatataataa atagtggagc aacagttctg
     2701 cgtgaagcgg gtcaggggag gaatccagca gccctaagcg atttgaattg tgtgctcttt
     2761 tttcgtgcac tttaaaaacc ctttaaaatc aacactttaa ggggtttttg tttgtcttgt
     2821 ataagaaaaa ggggcagacg aggggcacaa tttaaaattt tatcttgtct aacttgctag
     2881 atatgtctga taccattttt tgagtaacgt gagaataaat ctctagtgtg gtctttgagt
     2941 cactatgccc tactctgtcc atgatggcag tcaagggaat gcccagctca gcaagtaggg
     3001 atatgtgaga atgtctaaat gtatgtgtag ttatgttttt ttctatgccg attttttgac
     3061 catgtctttt caatgcacaa ataaccctgg catttgttat tggttctcct agagtattga
     3121 tgaaaataaa atctgtatca aatccatttg tcgcattctc tattatttgc tctttgataa
     3181 tatctaacac tttttgaggt gctgttataa ccctatcgga cttgattgtc tttggtgtag
     3241 ttctctcttt ttgtctgaaa tcgtatgtat gcttgatgtg aatagtcttt ttagaaaaat
     3301 ctatatcttc cttgtagttt aaggctgcca gctctccata cctcatgcca gtaagaaaaa
     3361 gaactttagc tattcggata tactttgtaa ttcgataatc acatagggcc tcgtctttta
     3421 aattttggat gaataactta aactcttttt ggtctaagta ttttgtattt ttctttctga
     3481 gttcgtcgga tgtaattact tttctaggcg tttcaacaaa tagcatttca tttgtatcaa
     3541 tataattcat tctgatagca aatttcatta tctgattgag cttgaatttg attttagaaa
     3601 catagttatg agatctccca tcttgtaata gttgatctat tactttttgt aataaacgtc
     3661 tatcaaattt ctaactaggt agtcgccctc tatctgcttt aaaatctctt tttttacatt
     3721 ctttgaagcg tagacggttg agttttttac gccgtgtttc caattctcct caaattcttc
     3781 atatagtttt tcaaaagtta tattagaaac agaatgttgt ttttctccta acttttgttt
     3841 tatcttttcc tgaagtaaaa tcgcagcttg atttcttgct tggggagttt ttttctccat
     3901 cgttactgag acttttttta atttctcagt atatggatct ttgtatctct caaaaaattt
     3961 atatttgccg tttggcaatt cttccatcca cattgatttt acctcacttt tttgataaaa
     4021 tgggtatagt aaagagggct ttttttatta tgagaatttc ttgattactt ctaaaatttc
     4081 attggcataa tttgcgactt cgagaggggt agacaccggg aagatgcctt tatcacggat
     4141 ttcgatagtg cttttttttg aatttgaacg ataacgtaat acccattttt tgatgttatc
     4201 gtcaaccaaa acattgaaat agctacggtt atctctgtaa aatacacgtt ctggagaaac
     4261 tacatcccta gcaagcattt taacaacagt ataaacttct aattcagcag gagttgtgat
     4321 aatttcgtca gttacttcga caatctcttc agcttcagct tcaacttttg gaatatcggt
     4381 tgttactttg gtttcaacgc ttgtattaag tgcagcactt aatttttcat taactcgctc
     4441 tgtgatgaat tgattaaacc ctttcacaat aatgggagaa aatgtcgtta agatattttg
     4501 agtaacacgc ccttcataga tttcagaagt tagatatctg aggaagctat ctgacggtgt
     4561 agtgatattt tcagtaagga acgctttaag attgttgagg tatttcaatt cagaagctga
     4621 tgaaacaata ttatcaatat caaaattctc tttgtggaat ttgatgattt cagtaaattg
     4681 attttctttg atatcagtca catcgattgt taagaatgga gttgtgtcca ttttatttgg
     4741 ctcatctaaa tcagtaaaga atttatattc tctaccattt gtcaagatac cgaattttga
     4801 tttagtagtt acgaaatatc tgaatagttg agagtcgtgc ttagtaaggt tttctgtaat
     4861 tgatttacat tcaattagga tttgaggctc gccatccaaa atgatcgcat agtcgacttt
     4921 ttcgcctttt ttaatgccca cgtcggcagt aaattccggg acaaattcaa gtggattgaa
     4981 gatatcatat ccaagcgctt ggaagaacgg cataatgaag gcattctttg tttgttcttc
     5041 attcgtaatg ctttggctga ggtctgctac acgtttgccg acttgtttta aatcggcttt
     5101 tactttatca atttccatat tgatactcct ttttttaatt tactaatgct aaatactctt
     5161 ctttaaccat gatttcattt gtcatggttt ttagattgta gtaagacatg aatttgaggt
     5221 aatcaaactc tgtggggtcg tctaagtttt ctagagcgtc ttttacgaga tgatggatca
     5281 tattcctatc agcttcgttt tcacagcgta gacgggcgtt ctggtgctct gagcgtgtgt
     5341 ggtctttgtg tccaagttcg tgtagggcga cttggatttg ttgttcagga gtcaagttgt
     5401 ggtcaatagc gagcacgttt gtgtctggat tgtagaaacc gctattgtgc cagtttgaac
     5461 cgtcgaagag acaaagttct acttgatatt cttcgcagag tttagcgagt gtcatagttc
     5521 tccttcgtta tttatagaca tctctgcggt gggcgatttc cacggctagg acgactagtt
     5581 tatcgtcttg gatatcacag atgatgcggt aattctctac tctgtatcgc cagtaacctg
     5641 caaggttggc ttttagtgct tttccgtgtt gtcgtggatt ggtcgtgttt tcaatgtttt
     5701 tagcaagcca ggataggatt tgttttctgg ttggggtatc tagtttttta agttgcttga
     5761 gagctttttt atcaatatct agccgataca ttaagcaata tcctctcgag ttagtcctag
     5821 ttcatccaag acctcatcca tggtataagt agctgggtcg gctaaatact ccatataggc
     5881 ttggtcggct gctcgtgcgt cttcgatatc ttccatgagt gccatgaagt cgtcaaaatc
     5941 catggtcgtt gtgtcgatac cgtgtttgtt taggtagtcc gtaatgtagg aatttttttc
     6001 tgtgaagttg atagtgatag tcattagcgt tctcctttgc ttttgaagtg ggcggatagg
     6061 acggatgtaa tgaagtcgat atcatcttca tttagtggtt tcccgtcaaa taacatggtg
     6121 ttggctgctg ctttgcgtaa gtctatgatt tgtccgttta cttgggcaaa ttcatcactc
     6181 ccagcgatag cagggttatc tgttcgtccg agcaggtagt cggtggatac gttgaaataa
     6241 ttagcaattt ctgcgatacg ttcagcgttt ggcgtagaat tttttatctt atagagtgta
     6301 tttctaccat aacctaagtc ttcttctact tgtccaagag cttttccacg cttttttgcc
     6361 aattctttta ttttttcaaa tgtctcaaac attgttaaat caacctttct aagacatcac
     6421 aaaaaaaatt taacaaattt ggtgtaaaaa ggttgactaa ttatcccaaa aggtgtaaaa
     6481 tgttttttgt aagtgataaa caactaaaaa aacaactaag aaataaatta taaaaatgtt
     6541 ttggcgaa
//
LOCUS       DAVMGZ010000103         6455 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000103 DAVMGZ010000000
VERSION     DAVMGZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.104"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            119..1069
                     /locus_tag="VYQ93_001634"
     CDS             119..1069
                     /locus_tag="VYQ93_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV3336289.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYDDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPSSLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1246..2253
                     /gene="gap"
                     /locus_tag="VYQ93_001635"
     CDS             1246..2253
                     /gene="gap"
                     /locus_tag="VYQ93_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV3336290.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2321..3157)
                     /locus_tag="VYQ93_001636"
     CDS             complement(2321..3157)
                     /locus_tag="VYQ93_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV3336291.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3283..5478
                     /locus_tag="VYQ93_001637"
     CDS             3283..5478
                     /locus_tag="VYQ93_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV3336292.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQSLAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5531..5683
                     /gene="rpmG"
                     /locus_tag="VYQ93_001638"
     CDS             5531..5683
                     /gene="rpmG"
                     /locus_tag="VYQ93_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV3336293.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5693..5869
                     /gene="secE"
                     /locus_tag="VYQ93_001639"
     CDS             5693..5869
                     /gene="secE"
                     /locus_tag="VYQ93_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV3336294.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5924..>6455
                     /gene="nusG"
                     /locus_tag="VYQ93_001640"
     CDS             5924..>6455
                     /gene="nusG"
                     /locus_tag="VYQ93_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV3336295.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         2004 a         1123 c         1405 g         1923 t
ORIGIN      
        1 gaggctcttt tgtcttgttc ttgtttcaat tgactatagt tcacggatac agttgggaaa
       61 gaagctatta aatatttgga gagtcctttt gggatgaaca tgtcaaatat tactatagat
      121 gggcaagaaa agatgggcaa gaaatggatc tgagtcaaac gatgcttctt atgctcaagt
      181 agtgagtctt tatgatgata caagtatctc tgttagtaat aatgagacgg acaaagtgtt
      241 gactggatcc ctctatactg aaacaaatga acagggatta acgattccaa gcagtttact
      301 aaaaaattgg aatgaacaga caggaaaaaa tttgacaact aatgatctta ttggcaaatc
      361 agtctcagcc agcattgtag aaagtgctgc cgaaactagt aagattgctc aatttcaaac
      421 gaagattgta cgtgtaatca atgatgaaga tgacatggag gacagcaaca gtttcatgct
      481 gtctcatcaa atggaaacga ttttgaaaga ggctggattt acgaaagctg tatcttattt
      541 tatcttggaa ctcaaagatc catcacagac aaaagtagta acagaagaat tacagaaaaa
      601 taagaagtat actgtgcttt ctcaacagag agttcttgat attgtgatta cctttattcg
      661 tgttattcag ggattattga ttgtgctttc atcacaagct attgtggtag cggcggttat
      721 gattggtatc attatttaca tcaatatcat gcaacgttcc aaggaaatag gtgtcatgaa
      781 agcagttggt tatcagaatc gtggtgtcaa aggaattttt atttacgagg ctatctggat
      841 tgtaggcatc gccttgctgc tggcattttt ggttgcacaa ggggtgggaa gtttggcgaa
      901 tgcgattgta agtcactttt acccatccat cactaaggtt tttgaattaa atcttttatc
      961 tgttttagga actctagttt tcgctctatt acttggttat gtctcagcct acttcccggc
     1021 gcgtaagatt agtaaaatgg atcctgtaga atcgttacgc tatgaataat aagggattcc
     1081 ttgatttaca gtcttaaaaa gaaactcaca tagaaaaaca atggccatac ttgcaattca
     1141 actcaaatag ttgtataata ggtttgttga aaggtgattt gtagtgattc aagttactct
     1201 tttcacaata aaaatttcat gattttcata aggaggaaat cactaatggt agttaaagtt
     1261 ggtattaacg gtttcggacg tatcggtcgt cttgctttcc gtcgtatcca aaacgtagaa
     1321 ggtgttgaag ttacacgcat caacgacctt acagatccag ttatgcttgc acacttgttg
     1381 aaatacgaca caactcaagg tcgtttcgac ggtactgttg aagttaaaga aggtggattc
     1441 gaagttaacg gtaaattcgt taaagtttct gctgaacgtg atccagagca aatcgactgg
     1501 gctactgacg gtgtagaaat cgttcttgaa gctactggtt tctttgctaa gaaagaagca
     1561 gctgaaaaac accttaaagg tggagctaaa aaagttgtta tcactgctcc tggtggaaac
     1621 gacgttaaaa cagttgtatt caacactaac cacgacgttc ttgacggtac tgaaacagtt
     1681 atctcaggtg cttcatgtac tacaaactgc ttggctccaa tggctaaagc tcttcaagat
     1741 aactttggtg ttgttgaagg attgatgact actatccacg cttacactgg tgaccaaatg
     1801 atccttgacg gaccacaccg tggtggtgac cttcgccgtg ctcgcgctgg tgctgcaaac
     1861 atcgttccta actcaactgg tgctgcaaaa gctatcggtc ttgtaatccc agaattgaat
     1921 ggtaaacttg atggatctgc acaacgcgtt ccaactccaa ctggatcagt tactgaattg
     1981 gtagcagttc ttgaaaagaa cgttactgtt gatgaagtga acgcagctat gaaagcagct
     2041 tcaaacgaat catacggtta cacagaagat ccaatcgtat cttcagatat cgtaggtatg
     2101 tcttacggtt cattgtttga cgcaactcaa actaaagttc ttgacgttga cggtaaacaa
     2161 ttggttaaag ttgtatcatg gtacgacaac gaaatgtcat acactgcaca acttgttcgt
     2221 actcttgaat acttcgcaaa gattgctaaa taattcttga gttgatagaa agcaaggctt
     2281 tgtggtcttg cttttttata tggaaaaatg gatgacacga tcatccattc ttttttaatt
     2341 ctttttcaaa tgtatttgaa agggtagtga aagttagcct ctctaaagta agtgggtggg
     2401 taaaggaaag tcggaaggca tgaagcataa gccggcttgt ctttgattta ctattataga
     2461 gagggtctcc caggatagga agattatgat gcaaaaggtg cacacgaatc tgatgggttc
     2521 gccctgtctt tagcttgcaa tgagccaagg aagtcttgtt tgagaattgc tttaatctgc
     2581 ttacatgcgt ttcagcatat ttcccatttt ttgcatcaac tattcttttt ctacgatcat
     2641 ggcgatcacg tccaattttg tctctgaaaa caagttcttt tctgttgata tttccatcaa
     2701 ctagagccca atattctcta gaaatctctt ttttctccaa taagcgattg agaatgggca
     2761 ggataaaagg atttttggca aagagaacta agccactggt ttccatgtcc agacgatgaa
     2821 cgacatagca ggtttggcca acataggtac tgacatggtt aagaagggca atttcgtttg
     2881 gttgattacc atgcgttttc atcccctctg gtttgtttac aataatcaag tgttgatctt
     2941 gataaacttc ctgcactaag tctgggttgc cccaagggat cgtcttttgg ggataatctt
     3001 cctcgtcaaa agtcaactgg caaacatctc caggatttac gatttcgttc cagcggactt
     3061 cttcttgatt tatcaaaata tgtttcttga ttctcaaaaa atgacggatt tttctaggga
     3121 tgaggagttg ttcctcaagt aattgcttta ccgtcatttg aggtagagag gcgggtaatg
     3181 taaatgtgaa ttgcatacag atattgtaac aaaaaaagcc ctatttggat aggaaatagc
     3241 taaattcttg tcttcctatg atgaagatga taaaataaac gcatgaaatt agataaatta
     3301 tttgagaaat ttctttctct ttttaaaaaa gaaacaagtg aactagagga ctctgattct
     3361 actatcttac gtcgctctcg tagtgatcga aaaaaattag cccaagtagg tccgattcga
     3421 aaattctggc gtcgttatca tctaacaaag attatcctta tactaggttt gagtgcaggc
     3481 ttgctagttg gaatctattt gtttgctgta gccaagtcga ccaatgtcaa tgatttgcaa
     3541 aatgccttga aaactcggac tcttattttt gaccgtgaag aaaaagaggc tggtgccttg
     3601 tctggtcaaa agggaaccta tgttgagctg actgacatca gtaaaaactt gcagaatgct
     3661 gttattgcga cagaagaccg ttctttctat aaaaatgacg ggattaacta tggccgtttc
     3721 ttcttggcta ttgtcactgc tggacgttca ggtggtggct ctaccattac ccaacagctg
     3781 gctaaaaacg cctatttatc gcaggatcaa actgttgaga gaaaagcgaa agaatttttc
     3841 cttgccttag aattaagcaa aaaatatagt aaggagcaaa ttctaaccat gtaccttaac
     3901 aacgcttatt ttggaaatgg tgtgtggggt gtagaagatg cgagtaagaa atactttgga
     3961 gtttctgcat cagaagtgag tctggatcaa gctgcgactc tggcagggat gctcaagggg
     4021 ccggaactgt ataatccctt gaattccgta gaagattcta ctaatcggcg cgatactgtc
     4081 ttgcagaata tggttgcagc aggatatatt gataaaaacc aagaaaccga agctgctgaa
     4141 gttgatatga cttcgcaatt gcacgataag tatgaaggaa aaatctcaga ttaccgttac
     4201 ccctcttatt ttgatgcggt ggttaatgaa gctgtttcca agtataatct aacagaggaa
     4261 gagattgtca ataatggcta ccgcatttac acagagctgg accaaaacta ccaagcaaat
     4321 atgcagattg tttatgaaaa cacatcgcta tttccgaggg cagaggatgg aacgtttgct
     4381 caatcaggaa gtgtagctct cgaaccgaaa acagggggag ttcgtggagt tgtcggtcaa
     4441 gttgctgaca atgataaaac tggattccgg aatttcaact atgcaaccca atcaaagcgt
     4501 agtcctggtt ctacaattaa gcctttagtt gtttatacac cagcagttga agcaggctgg
     4561 gctttgaata agcagttgga taaccatacc atgcagtatg atagctataa ggttgataac
     4621 tatgcaggga tcaaaacaag tcgagaagtt cctatgtatc aatccttggc agaatcgctt
     4681 aatctacctg ctgttgccac tgttaatgat ttgggtgttg acaaggcttt tgaggcaggc
     4741 gaaaaattcg gactcaacat ggaaaaggtc gaccgtgttc ttggtgtcgc cttgggaagc
     4801 ggtgttgaaa ccaaccctct tcaaatggct caagcatacg ctgcctttgc aaatgaaggt
     4861 ttaatgcctg aagctcattt tattagtaga attgaaaatg ctagtggaca agttattgcg
     4921 agtcataaaa attcacaaaa acgggtgatt gataagtctg tagctgacaa gatgaccagt
     4981 atgatgttgg ggactttcac caacggtacc ggtattagtt catcgcctgc agactatgtc
     5041 atggcaggga aaactggaac aactgaagca gttttcaatc cggagtacac aagtgaccag
     5101 tgggtaattg gttatactcc ggatgtagtg attagccact ggcttggctt tccgaccact
     5161 gatgaaaatc actatctagc tggctctact tcaaacggtg cagctcatgt ctttagaaac
     5221 attgccaata ccattttacc ttatacgcca ggaagtacct ttacggttga aaatgcttat
     5281 aagcaaaatg gaattgcacc agccaataca aaaagacaag tacaaaccaa tgataatagc
     5341 cagacagatg ataatttgtc tgatattcga gggcgtgcgc aaagtctagt agatgaggct
     5401 agccgggcta tctcagatgc gaagattaag gaaaaggctc aaacaatatg ggattcgata
     5461 gtcaatctat ttcgctaaga tgcttgtcaa agcctagctt tcttgttata atggataaga
     5521 tggaggcgtt atggcactaa aaaaagcaag cctagcttgt gcggtttgtg gttcgagaaa
     5581 ctattcaatc aagatcagcg gaaaccccaa gcctacacga ctagaagtaa ataaattttg
     5641 taagcattgt ggcaagtaca ctacacacag agaaacgaga taggagagag cgatgcgttt
     5701 tattggagat atttttagac ttcttaaaga cacaacatgg ccaactcgca aggaaagctg
     5761 gagagatttt cgttctatca tggaatacac agctttcttt gtagtaatta tttacatttt
     5821 tgaccagttg attgtttcag gtttgattcg atttattaac attttttaga agattagtgg
     5881 agttaattac actagaaatc ttctatttat gaaaggaaat atcatggata gttttgataa
     5941 aggatggttt gttttacaaa cttattctgg ttatgaaaat aaggtgaaag aaaatctatt
     6001 acaacgtgca caaacctaca atatgttgga taatattcta cgcgttgaaa ttccaacaca
     6061 aacagtgcaa gttgaaaaaa atggaaagag aaaagaagta gaagaaaatc gctttccagg
     6121 ttatgttctt gtagaaatgg tcatgacaga tgaagcttgg tttgttgttc gaaacacacc
     6181 aaatgttaca ggatttgtcg gatcacacgg gaacagatca aaaccaactc cattattgga
     6241 acaagaaatt cgtgacattt tggtatctat gggacaaact gttcaagaat ttgatttcga
     6301 tgttgagatt ggtcaaactg tacgtattat tgatggtgct tttgcagact acactggtaa
     6361 gattacagaa attgataata acaaagtgaa aatgattatc tctatgtttg gtaatgacac
     6421 agttgcagaa gtaaacctaa accaaatcgc agaat
//
LOCUS       DAVMGZ010000104         6436 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000104 DAVMGZ010000000
VERSION     DAVMGZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.105"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            4..315
                     /locus_tag="VYQ93_001641"
     CDS             4..315
                     /locus_tag="VYQ93_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336296.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            319..1035
                     /locus_tag="VYQ93_001642"
     CDS             319..1035
                     /locus_tag="VYQ93_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV3336297.1"
                     /translation="MKKYFISGLGSNAYHSKDFFQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALKELPDYFNTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(1165..1980)
                     /locus_tag="VYQ93_001643"
     CDS             complement(1165..1980)
                     /locus_tag="VYQ93_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV3336298.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(1977..2882)
                     /locus_tag="VYQ93_001644"
     CDS             complement(1977..2882)
                     /locus_tag="VYQ93_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV3336299.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMLFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            complement(3153..3236)
                     /locus_tag="VYQ93_001645"
                     /pseudo
     CDS             complement(3153..3236)
                     /locus_tag="VYQ93_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            3280..5409
                     /locus_tag="VYQ93_001646"
     CDS             3280..5409
                     /locus_tag="VYQ93_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV3336300.1"
                     /translation="MDKKYEKISQDLGVMLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            5396..5845
                     /locus_tag="VYQ93_001647"
     CDS             5396..5845
                     /locus_tag="VYQ93_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV3336301.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTQRYRCGLCRGKLLLVNQPED"
     gene            5903..6175
                     /locus_tag="VYQ93_001648"
     CDS             5903..6175
                     /locus_tag="VYQ93_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV3336302.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
BASE COUNT         2013 a         1173 c         1409 g         1841 t
ORIGIN      
        1 cgaatggaat taaaagattt tacagaaaaa gaacaggaaa tgattaagaa aaggcttaca
       61 atgtctaata ttagtgacaa agaaactact gagaagattc tggcgctagt accacaagac
      121 ttgattaagc gaatcccgtt ttttgtcaga aaacatgcta caacacgtac gattaaacgc
      181 atttcaattg aataccctga actctacgct gtagctcaaa caagtggtga gattccagaa
      241 aaagaacgcg aagaattgcg tcagattatc acgactatct ttgaacaaaa gatgaataag
      301 catagtatta agtagagaat gaaaaaatat tttattagcg gtttgggaag caatgcctat
      361 catagcaagg atttttttca agaactagat tcgcaggtct attttctaaa tccatatgaa
      421 aagcatcttc gagatgaaac agaattgaaa tcatggttta aaaatgagat tgtagaggaa
      481 gaatctatct gtctgatagg ccattctctt ggaggagatt tagctcgtta tttcgcatcg
      541 gaatttgaag aagtaaagaa actgattctt ttggatggtg gctatctaga tttagataag
      601 attttacctt tggatacaga gttagaggaa actaaaaatt atatcaaatc tcaaattgtt
      661 tcggacttag ctcttcttat ttctaaagag aagtccgaag caaagcattg gtcagaaaat
      721 atggagaaag ctgtaagaca gtcctatcac tggaatgttg agtataatag atatgagttg
      781 gctataaatt atgaaaatat agaagcgata ctccgcctac ggagaaaaat acaagctttt
      841 aagagagaag tgggagatac cttgtttatt agtcctcgct atcctaatgg agctacatgg
      901 agagaggaag ccctaaaaga attgccagac tattttaata ctattttttt agaaaacttt
      961 ggccatgagc tttatactca agcacctaaa gaaatcgcta gtctaatgaa tgagtggctc
     1021 gcttattttc tatgagctac ttgtattgct ggaaatggta taagatgaaa atctacaagt
     1081 cactaatttt catataacaa aaaaacgaac aggctctttt tctgttattc agaagagaac
     1141 ttgttcgttt ttatcttgat tttcttaaaa agctctatat acatagggat tttctggttt
     1201 atcgactttg gtaaagctgt cgatttccaa ggttggaagg ttttgtttga gttctttatg
     1261 gtagtgattg accagttttc ctttggctgt aatccaggtg ttgttttcat actgccgggt
     1321 attgcccttg gtcagcaatc catagacacc agaatctgcg atacagtgga taatgccgaa
     1381 tcggaacaga aattgactat tggcatgact ggggtcgtta tagacaaagc ctgtaaactg
     1441 gattgtcttg ccctcaaatt catctggata gtcgtagata gcctccatga cttccatata
     1501 gttttcatta gtgatctgaa tactatcttg ggataagtat ttatccgccg ccgttcgcat
     1561 ttccttttca taggctgatt ttgaaaaata agaactagta tctggtttca aatattggct
     1621 tgtcgtccct tcacttgtct gaatagctag atccgttcct tccgataggg ggaaatgata
     1681 acctttagca gaaacggtct gagaatccaa gctaacagtt gggaaagtta agccgatgac
     1741 aagtggaata gccagaagag aaatactcgt catcttggcc aatcggctgt tcagatgact
     1801 gtgggttttg acttgcttca tccagatata caattgaaca attgccaaga taaaggaaag
     1861 caccatggag atataggcca gataggaata gtgcatgttg atgtactggt ttagtttgcc
     1921 cgacagatgg aggtaaatag tcagttcaaa atagccagct aaaactaaaa atcgaatcat
     1981 aggatgactc caatcaagag agaataggct aaaacgacca gagtcacaat cgtaatgaag
     2041 tgactaataa atcgtgcttt caagtaattt ttcatcatga gaatattttt gatatccagc
     2101 attgggccaa tgacgagaaa ggccagaact ggtgccaaac cgaaactcga gagaagagaa
     2161 gcacctataa aggcatccgc ctcactacag agcgaaagaa gaaaggccaa aatcatcaag
     2221 agcaggatgg caaaaagagg agtcgcactg atagaggtca gaatccgagt cggaacgtag
     2281 acctgtatta tagaagcaaa gaggcagcca aataccaaat aacgccccgt atcaaaaaat
     2341 tcatcaatgg cctgcacaaa gacttgaaaa actttttttg cagaactcaa gtaagaaaaa
     2401 tcatgctcat gacaagccag acgattttct ttctgaatcg gttcttgcca gaaaaatcct
     2461 agaaaaattc ctagtattac agccacaaga atggaaccca gagctcgtaa tagggcgaca
     2521 tggaaggagt tgccaaaggc agaataggtc gcaaaaagaa caatgggatt gataacaggt
     2581 gctgtcacaa gaaaaggaac ggctgtgtaa ctgggaacct ttttttccag aaaacgattg
     2641 atgatgggga cgattccaca ttcacaagaa gggaaaagca taccgacaaa ggtcccaaaa
     2701 aagattctcc cccaacgatt tcgagggaga aaatgataaa ccttgtcagg tgtgatataa
     2761 acttcaatca gccctgagac aatgcttcct attagaacga agggaagggc ttcaatgata
     2821 atggagagaa aaatggctcc agtttgaagt acactagatg ggagttgttg aaaagcagtc
     2881 atctagtttt tcttttctgc tttggctttt ttctctttgt catcagggaa agtaacttgt
     2941 ttcaaatcag ggagtttagc aaactcttca aacatcttgt ccaagtcgtc tcgttttgta
     3001 aattttacag ccataggtgt acctcgattc taaagtctac ctctattata ctattatttt
     3061 gcggaaaagg gagtttttta atttgttttc gatagttgat acacttcgaa aatctcttca
     3121 aaccacgtca gcttcgcctt accgtatata tgtgacttat ccacaacctc aaaacagtat
     3181 tttgagcagc ctgcggctag cttcctagtt tgctttttga ttttcattga gtatgaaaag
     3241 gattggagaa agccttccag cttgtggtat aatagattta tggataaaaa atatgaaaaa
     3301 atctctcagg atttgggagt gatgttaaag caaattgata ccgttctaag tttgacagct
     3361 gaaggggcga ctattccctt tatcgcgcgt tatcgcaagg acatgactgg tagtctggat
     3421 gaggtggcga ttaaggctat tattgatttg gataaaagtc tgacaaatct caatgaccgt
     3481 aaggaagctg tcttagctaa gattcaagaa caaggtaagt tgaccaagga attggaagaa
     3541 gctatcttag ttgccgaaaa attagcagac gttgaagaac tctatcttcc ttataaggaa
     3601 aagcgtcgta ccaaggcaac cattgcccgt gaagctggac tctttcctct tgctcgtttg
     3661 attttgcaga atatagttga cttagagaaa gaagctgaaa agttcgtctg tgaaggattt
     3721 gcgactggca aggaagcctt gaccggtgca gttgatattt tggtcgaagc cttatcggaa
     3781 gatgtgacct tgcgttctat gacttatcag gaagtgctga gacactctaa acttacttct
     3841 caagccaagg atgaaagtct tgatgaaaag caggtttttc agatttatta tgatttttca
     3901 gagacagttg gaactatgca aggctatcgt accttggctc tcaatcgtgg ggagaaactt
     3961 ggtgtcttga agatcggttt tgaacatgcg acggaccgta ttcttgcctt ctttgctact
     4021 cgtttcaagg tgaaaaatgc ttatattgat gaagttgttc agcaatccgt taagaaaaag
     4081 gtcttgcctg ctattgagcg tcgtattcgg acagaattaa ctgagaaagc tgaagaggga
     4141 gctatccaac ttttttctga caatctgcgc aatctcctct tggttgctcc attgaaaggg
     4201 cgcgtggttc ttgggtttga cccagccttt cgtacaggtg ccaagctagc tgtcgtggat
     4261 gcaacaggaa aaatgctgac aactcaggtc atctatcctg ttaaaccagc atcagctcgt
     4321 caaatcgaag aagccaagaa agatttagca gatttaattg gtcaatacgg tgtagagatt
     4381 attgccattg gaaatggaac ggccagtcgt gaaagtgaag cttttgtagc ggaagttctg
     4441 aaagatttcc ctgaagtcag ctatgttatc gttaatgaaa gtggtgcttc tgtctattct
     4501 gccagcgaac ttgctcgtca ggagtttcca gacttgaccg ttgaaaaacg ctctgccatt
     4561 tctatcgccc gtcgtttgca agatcctctt gcggaattgg tcaaaatcga tcctaagtca
     4621 attggtgtcg gtcaatacca acacgatgtc agtcagaaga aactatctga gagtctggac
     4681 tttgttgtcg atacagtggt taaccaagtt ggtgtcaatg tcaatacagc tagcccagct
     4741 cttctttcac acgtagctgg actcaataaa accatttctg aaaatatcgt caaataccgt
     4801 gaggaagagg gaaaaatcac ttcacgctcc caaatcaaga aagttcctcg tttgggggcc
     4861 aaggcttttg aacaggctgc tggtttcctc cgtatccctg aaagtagcaa tatccttgat
     4921 aatacaggag ttcacccaga aaattacgct gccgttaagg aactcttcaa atgcttggac
     4981 attaaggatc tgaatgaaga ggcccaaagt aaactcaagt cgctttcagt caaggaaatg
     5041 gcgcaagagt tagaccttgg tccagaaact cttaaagata tcattgcaga tcttctcaaa
     5101 ccaggtcgag atttccgtga ttcctttgat gctccagttc tccgtcaaga tgtcctagat
     5161 atcaaggact tagtggttgg acaaaagcta gaaggagtgg tgcgtaatgt tgttgatttc
     5221 ggtgcttttg ttgatatcgg aattcatgag gacggtctca tccatatttc ccatatgagt
     5281 cgcaagttta tcaaacaccc tagtcaagtg gtggcagttg gagatttggt gagcgtttgg
     5341 gttaagaaaa ttgatactga acgtgaaaaa gttaatctgt ctctcctagc tccaaatgaa
     5401 actgactgat tacgtcaagc aggtttcact agaagacttc ggcagacctt ttatccatca
     5461 tgtccagtgg aataggcgtc tacgttcgac aggtgggcga tttttcccca aagatgggca
     5521 tttggatttt aatcccaagg tttatcagga actggggttg gacgttttta ggaaaattgt
     5581 ccgacatgaa ctctgtcatt atcacctcta ttttcaaggg aaaggctatc aacacaagga
     5641 tcgggatttt aaggaactgt tgaaagcagt ggatggatta cggtttgtac catccttgcc
     5701 caatagtaac tccaagccaa tcaagctcta tcgttgccaa tcctgccagc aaacttatca
     5761 gcgtaagcgt aggattgata cccaacgcta tcgctgtgga ctttgtcgag gaaagttgct
     5821 attagtaaat cagcctgagg actgatgaaa gccgagccta tccgtgatat actagtctaa
     5881 cctagcagaa agaggaaata tcatgaacag taagttttat aaaatgagac gaaatcgcat
     5941 gatttcagga gttttagcag gtctgtcaga caagtggaac tttgatgtta cccttgtacg
     6001 ttttctgttt gccattttta cagtcgcaaa ctttggactt ggggtgatta tctatatcat
     6061 cctagcttct atcatgccaa ccaaggaaga aatcgaagca gaaatgtacg gaacaggccc
     6121 acgtaaacgc aaagaagctc aagccattga cgataatgaa ggctggtttt ggtgaggttt
     6181 tacaaaagat ttggaatacg caaaaagctt taaatcaata gtctaaactg cttgatttaa
     6241 agctttttta cttttatact caataaaaat caaagtgcaa actaggaagc cagccgcagg
     6301 ctgtaattta gtcggcaatg tgaagttgat atagtttgca ctttgatttt tgaatagtat
     6361 taataatatt tcgccagctt catccacaaa ctatagtgga ttggaactag aataggacac
     6421 ctatacttct aaaaaa
//
LOCUS       DAVMGZ010000105         6065 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000105 DAVMGZ010000000
VERSION     DAVMGZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.106"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            101..1168
                     /locus_tag="VYQ93_001649"
     CDS             101..1168
                     /locus_tag="VYQ93_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336303.1"
                     /translation="MTIISMIITNLRTLYVYVLQMTNRLKDSSIILLSDRVLYVLLLF
                     LFIVFGWHEYKVMILADILGRSFSLILSFWICKDIVFQPLSKFIFNIKESFDNIRVGI
                     NLMLSNIASSLIIGIVRLGIQWNWNIETFGKVSLTLSVSNLLMTFINAIGLVVFPLLR
                     RTKAENLPKIYSNLRNVLMLIMFAILLIYYPLKIVLDLWLPAYQDALIFMTLIFPMSV
                     YEGKMALVINTYLKALRMERDILRVNALVMLISMGVTLVTTYLLNSLELTVVSIVVLL
                     ALRSIIAELILSKKLDVSVKKDIVLEFLLTLVFISSSWYLPIGLAVIVYTIAYGLYLY
                     LKHEDIKTYLAYFKASKKTSN"
     gene            1233..2321
                     /gene="wecB"
                     /locus_tag="VYQ93_001650"
     CDS             1233..2321
                     /gene="wecB"
                     /locus_tag="VYQ93_001650"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEV3336304.1"
                     /translation="MKKIMLVFGTRPEAIKMCPLVNELKKHEDMETIVCVTGQHKEMV
                     SPVLDLFGVVPDYDLEIMKANQTLFSITTSILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIATYHFAPTELAKENLLKE
                     GRENVYVTGNTVIDALTTTVQEDYTHTHLDLNANNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYEDVKVIYPIHKNPLVRETAAEIFGDTERIQIIEPLDVLDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKLLLDDSG
                     EYKKMSQASNPYGNGDASQQIVQILRGI"
     gene            2484..3353
                     /gene="rfbA"
                     /locus_tag="VYQ93_001651"
     CDS             2484..3353
                     /gene="rfbA"
                     /locus_tag="VYQ93_001651"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV3336305.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSV
                     ALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEISYRMGYISREDVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            3354..3947
                     /locus_tag="VYQ93_001652"
     CDS             3354..3947
                     /locus_tag="VYQ93_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV3336306.1"
                     /translation="MSDNFFGKTLVVRKIDAIPGLLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADDGKVLGSWVDLREG
                     ETFGNVYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPALGIEWENLAEAEVSEADKHHPLLRDVKPLTKDEL"
     gene            3959..5005
                     /gene="rfbB"
                     /locus_tag="VYQ93_001653"
     CDS             3959..5005
                     /gene="rfbB"
                     /locus_tag="VYQ93_001653"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV3336307.1"
                     /translation="MTEYKKIIVTGGAGFIGSNFVHYVYNNFPDVHVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGLGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQPAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKETIKWYTDNQDWWKSEKE
                     AVEANYAKTQQVIK"
     gene            complement(5067..5921)
                     /gene="rfbD"
                     /locus_tag="VYQ93_001654"
     CDS             complement(5067..5921)
                     /gene="rfbD"
                     /locus_tag="VYQ93_001654"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV3336308.1"
                     /translation="MILITGANGQLGTELRYLLDERNVDYVAVDVTEMDITNSEMVEK
                     VFAEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKASEKYGAILVYISTD
                     YVFDGKKPVGQEWEVDDIPDPQTEYGRTKRMGEELVENLTSQHYIIRTAWVFGNYGKN
                     FVFTMQSLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYVTENQKEYGYYHLSNDAAEDT
                     TWYDFASEILKDSDVEVVPVDSSKFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALKE
                     FYKQEVRK"
BASE COUNT         1792 a         1119 c         1281 g         1873 t
ORIGIN      
        1 gatgattcta ttttctagct tcttaatgat tatctccttg ctattgtttg gtttaaatgt
       61 attagctgta acagattcga atgcacgata tatttataat atgactatta taagtatgat
      121 aatcacaaat ttaagaacgc tctacgtgta tgttttgcag atgacaaacc gcttgaagga
      181 tagttccatc attctactca gtgatcgcgt tttatatgtg ctccttttat tcctgtttat
      241 tgtatttggt tggcatgaat acaaggttat gattttggca gatattctag gtcgatcatt
      301 ttctctcata ctttccttct ggatttgtaa agatattgtg tttcagcctt tgtcaaaatt
      361 catctttaat ataaaagagt cttttgataa tatccgagtt ggtatcaatt taatgttatc
      421 caacatcgca agtagtttga ttatcggtat tgttcgtcta ggaatccaat ggaactggaa
      481 tatcgaaaca ttcgggaaag tatctctgac cttgagtgtt tccaatctat tgatgacttt
      541 cattaatgcc attggactag tcgtctttcc gctattaaga cgaacaaaag cagaaaactt
      601 acctaagatt tattctaatt taagaaacgt tttgatgctt atcatgttcg cgattttgct
      661 catttactat cctttaaaaa ttgtattaga cctctggttg ccagcctatc aagatgcctt
      721 gattttcatg acccttattt tccctatgtc agtctatgaa gggaaaatgg cattggtcat
      781 taatacttac ttaaaggcat taagaatgga aagagatatc cttagagtca atgccttagt
      841 aatgttgatc agtatgggag tgaccctggt tacaacatac ctgttaaata gtttggagct
      901 gactgttgta tcgatagttg ttttgctagc tttgagaagt atcatagccg aattaattct
      961 gtctaaaaaa ctggatgttt cggttaagaa ggatattgta ttagaatttc ttttgacgct
     1021 tgtctttatt tcttcaagtt ggtatttgcc gattgggctg gcagtaattg tctacacaat
     1081 agcctacggt ttatatctct atttgaaaca cgaagatatc aaaacctatt tagcttactt
     1141 taaagctagt aaaaaaacat caaattaaaa aattatataa tgattaagtg gtagattcta
     1201 tttctaccgt tttagatatt cgggaggtaa tgatgaaaaa aattatgtta gtattcggta
     1261 cacgtccgga agccatcaaa atgtgtccat tagtcaatga gttgaaaaaa cacgaagata
     1321 tggaaacaat tgtgtgtgtt actggacaac acaaagagat ggttagtcct gttttagatt
     1381 tatttggtgt tgtaccagat tatgatttag aaattatgaa ggctaaccaa accttgttct
     1441 ctatcacaac tagtatcttg gaaaagataa aaccagtttt agagaaggaa caaccagata
     1501 ttgtcctagt tcacggtgac actacgacaa cttatgcagc agccttggca gcattctatt
     1561 tgggaattaa agtaggacat gttgaagctg gtttgcgaac gtacaattta caaagtccat
     1621 ttcctgaaga atttaacagg caatcgacat caatcattgc aacttaccat tttgctccaa
     1681 ctgagttggc taaagaaaat ctcttaaaag aaggtagaga gaatgtttat gtgactggaa
     1741 atactgtcat tgatgctctt acaactactg ttcaagagga ttatacacac actcatttag
     1801 atttaaacgc taacaatcgt ctcatcttat tgactgctca tagacgcgaa aatctcggcg
     1861 aaccgatgag acatatgttt agagcagtta aacgagtatt gaatgaatac gaagatgtta
     1921 aagtcattta tccaattcat aagaatcctt tggtacgtga aacagctgca gagatctttg
     1981 gagatacaga acggattcaa attattgaac ctttggatgt tcttgatttc cacaacttca
     2041 tgaaccaaag ttatatgatt ttaacagatt ctggcggagt tcaggaagaa gcaccttctt
     2101 taggaaaacc tgtattggtt atgcgcgata ctactgaaag acctgaagga gtagcggcag
     2161 gaaccttgaa attagtagga actgacgaag aaacaattta tcaaaacttc aaactacttc
     2221 tagatgattc tggagaatac aaaaaaatga gtcaggctag caatccatac ggtaatggtg
     2281 atgctagtca acagattgtt cagattttgc gtgggattta agaagattct ctaaaagagt
     2341 ctaagaggaa tccactccac aaacataaaa ctcttatgct atcatttatg attgagatag
     2401 actcataact taatattttt atcctaagaa aattcgttca tattcaatat gaatgaaaaa
     2461 caacgaagaa aggttatttc attatgaaag gtattattct tgcaggcggc tcaggtaccc
     2521 gcctgtaccc acttactcgg gctgcgtcaa aacagctgat gccggtttat gataaaccta
     2581 tgatttatta tccgttgtcg acattaatgt tggctggaat taaagatatt ttgattatct
     2641 caactcctca agatttgccc cgttttaagg acttgctctt ggatggttcc gaatttggga
     2701 tcaagctttc ctatgcggaa caacctagtc ccgatggact tgctcaggct tttcttatcg
     2761 gtgaagaatt tatcggtgac gatagcgttg ccttgatttt aggcgacaat atctaccatg
     2821 gtcctggtct gagcacaatg cttcaaaaag cagccaagaa agagaaaggt gcgactgttt
     2881 ttggctacca agtgaaggat ccagagcgtt ttggtgtggt tgagtttgat acagacatga
     2941 atgctatttc tatagaagaa aaaccggagt atcctcgctc caactatgca gtgacaggac
     3001 tgtatttcta tgataatgat gttgtggaga ttgctaaaca gatcaaacct agtgctcgtg
     3061 gcgagttaga aattacagac gttaacaagg cttacctaaa tcgtggtgac ctttctgttg
     3121 agctgatggg gcgtggtttt gcctggttgg atacgggaac ccatgaaagc ttgctagaag
     3181 cttctcagta tattgaaacg gttcagcgta tgcagaacgt tcaagtggca aatctagaag
     3241 aaatttccta tcgtatgggc tacattagtc gtgaagacgt gctggaattg gctcagcctc
     3301 ttaagaaaaa tgaatacgga caatatttgc tccgtttgat tggagaagca tagatgtcag
     3361 ataatttttt tggaaagaca cttgtggtac gcaagattga tgctatacca ggactgctag
     3421 agtttgacat tcccgttcat ggagacaatc gtggttggtt taaggaaaat ttccagaagg
     3481 aaaagatgct accgcttggt tttcctgaaa gcttctttgc tgcagggaaa ctgcaaaata
     3541 acgtcagctt ttctcgcaaa aatgttcttc gaggactcca tgctgaacct tgggacaagt
     3601 atatctctgt tgcagacgat gggaaggttt taggatcttg ggtagatttg cgtgaaggcg
     3661 agacctttgg aaatgtttac cagacagaga ttgatgctag caagggaatt tttgtccctc
     3721 gtggcgtagc caacggtttt caagtcctct cagatacagt ttcctatagc tatctggtca
     3781 atgactactg ggctcttgaa ctcaaaccca aatatgcctt tgttaactat gctgacccag
     3841 cattgggaat tgagtgggaa aacctagcgg aagctgaggt ttcagaggca gacaaacacc
     3901 atcctctatt aagggatgtc aaaccactta cgaaagacga gttgtaaaag gaaacattat
     3961 gactgaatac aaaaaaatta tcgtgacagg tggagctggt tttatcggtt ctaactttgt
     4021 ccactatgtt tacaataact ttccagatgt ccatgtgaca gtgctggaca agctgactta
     4081 tgcaggtaat cgtgccaata tcgaggaaat tttaggcgac cgtgttgagt tggttgttgg
     4141 agatattgct gatgcagcct tggtagacaa gttggcggct gaagcggatg ctatcgttca
     4201 ctatgcggca gaaagccaca atgataactc gctcaatgac ccgagtccat ttatccacac
     4261 caactttatc ggaacttaca cacttttaga agcggctcgt aaatacgaca ttcgtttcca
     4321 ccatgtatcg actgatgaag tctatggtga cctgcctctg cgtgaagatt tgccaggtca
     4381 tggggaaggc ctaggtgaga aatttaccgc tgaaaccaag tacaatccaa gctcgcctta
     4441 ctcatcaacc aaggctgctt cagacttgat cgttaaagct tgggtgcgct catttggtgt
     4501 taaagcaacg atttctaact gttcaaacaa ctatggtcca taccagcata tcgagaagtt
     4561 cattccgcgc cagattacga atatcttgag cggtatcaag ccaaaactct acggagaagg
     4621 taagaatgtg cgtgactgga ttcacaccaa tgaccattca tcaggcgttt ggacgattct
     4681 gaccaagggt caaatcggtg aaacttactt gatcggtgct gacggtgaga agaacaacaa
     4741 ggaagtgtta gagctcattc tcaaggagat ggggcaaccc gctgacgctt atgaccatgt
     4801 gaccgatcga gctggtcacg acttgcgtta tgcgattgat gctagcaagc tccgtgatga
     4861 gttggggtgg aagccagagt ttaccaactt tgaagcaggc ctcaaagaga ccatcaagtg
     4921 gtacacagat aaccaagact ggtggaaatc tgaaaaagaa gcagtagagg ctaactatgc
     4981 taaaacgcaa caagtgatta aataataaga tactaaaaaa gcaagagacc tcgaggtctc
     5041 ttgcttttct agtatgtagt aagcttttat tttcttactt cttgtttata aaactcttta
     5101 agggcatctt gccaggttgg gatgacgaaa cctgttgcct ttgcctttgc caaactcata
     5161 gttgagttga gaggtcgttt agccttagcg gggaacttgc tagagtctac tggcactact
     5221 tcaacatcac tgtccttaag aatctcgcta gcaaagtcat accaagtggt gtcttcagct
     5281 gcgtcatttg ataagtgata gtagccatac tctttttgat tttcagtcac ataggtcatg
     5341 aattcggcca aggtccgtgt ccaagttggg cgaccgtatt ggtcgttgac cactgtgagc
     5401 gttttatggg ttttggctag gctttgcatg gtaaagacaa agttttttcc ataatttcca
     5461 aaaacccaag cagtacgaat gatgtaatgc tgtgacgtaa ggttttcaac aagttcttct
     5521 cccattcgct tggtacgtcc gtactctgtt tgcggatcag gtatgtcatc gacttcccac
     5581 tcttgtccta ctggtttctt tccatcaaag acgtagtctg ttgagatata gaccagaata
     5641 gctccgtatt tctcagaggc cttggctaca ttttcagttc cagttacgtt gatggcaaaa
     5701 tccagttctt tcccttcatc ttcggctgca tcaacagcag tgtaggctgc acaatgatag
     5761 actagagttg gcttaacctc agcaaagact ttttcaacca tttcagaatt agtgatatcc
     5821 atttcggtca catccactgc aacatagtcc acatttcgct catctagtaa ataacgtagt
     5881 tcggtaccaa gttgaccatt tgcacctgta attaagatca tatttttctc cttgatagtt
     5941 cttatactta attatatcaa aaatatagaa aaatcggttt ttagtgaacc cttttttggt
     6001 ataaactttg ttagtgaagc gcaagagagc cgaatagtta ttactttaga atatccttaa
     6061 aagta
//
LOCUS       DAVMGZ010000106         6007 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000106 DAVMGZ010000000
VERSION     DAVMGZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.107"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            128..1897
                     /locus_tag="VYQ93_001655"
     CDS             128..1897
                     /locus_tag="VYQ93_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEV3336309.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHINVLLGVLVAAVVSFLVA
                     ALILHTDKSTEDSLEAAQAATKAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            1921..3876
                     /locus_tag="VYQ93_001656"
     CDS             1921..3876
                     /locus_tag="VYQ93_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV3336310.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKLEEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            3878..4315
                     /locus_tag="VYQ93_001657"
     CDS             3878..4315
                     /locus_tag="VYQ93_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3336311.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4377..5513
                     /locus_tag="VYQ93_001658"
     CDS             4377..5513
                     /locus_tag="VYQ93_001658"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEV3336312.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT         1842 a         1006 c         1227 g         1932 t
ORIGIN      
        1 taatttttaa gcctttttgc ccagcctcgt caaaagtaat gttttgacac aaaatctgtg
       61 acaaaacttt agttttaaag gtttttaact ttgtatatac tagttttaag aaaaggagga
      121 tgatctaatg gaagaaaaag tatcattgaa agtcagggtt caaaaactag ggacatcgct
      181 ttcaaatatg gttatgccca atattggagc atttattgct tggggagtat tgactgccct
      241 ctttatcgct gatggctatc tgccaaatga acagttagct actgttgttg gtcctatgtt
      301 aacgtattta ttgccaatcc tgattggtta cacaggtgga tatatgatcc atggccaacg
      361 tggtgccgtt gtaggagcta ttgctactgt tggtgcaatt acaggttcta gtgttcctat
      421 gtttatcgga gctatggtaa tgggcccact gggaggatgg actatcaaga aatttgatga
      481 gaagttccaa gaaaaaattc gtcccggatt tgaaatgtta gttaataact tctcagctgg
      541 tctcgttggt tttgcattat tgcttttggc tttctacgca atcggtccag tcgtatcgac
      601 tcttactgga gctgttggga atggtgttga ggctattgtc aatgctcgcc tccttcctat
      661 ggctaatatt atcatcgaac cggctaaagt ccttttcctc aataatgccc tcaatcatgg
      721 catttttact cctctgggag tagaacaggt agctcaagct ggtaagtcaa ttctcttcct
      781 attggaagct aatcctggac caggcctggg aattctatta gcttatgctg tattcggtaa
      841 aggttctgct aaatcttctt cttggggggc aatggttatt catttcttcg gagggattca
      901 tgaaatttac tttccttatg ttatgatgaa gcctactcta tttttagctg ctatggcggg
      961 aggtatctct ggaactttta cttttcaact cttagacgct ggtcttaaat ctccagcttc
     1021 accaggttct attattgcga ttatggctac ggcgccaaaa ggtgtttggc cccatataaa
     1081 tgttcttcta ggtgttttag tagcagcagt tgtttctttc cttgtagcag cccttattct
     1141 tcatacagac aagtcaactg aggattcgct cgaagctgct caggcggcta ccaaagcagc
     1201 taaggctcag tctaaaggtc agttagtatc aacttctgtt gatgcagttg tttcgacaga
     1261 ctcagtggaa aaaatcattt tcgcctgcga tgctggtatg ggaagctctg ctatgggagc
     1321 tagtattctt cgagataagg ttaaaaaagc aggtctagag attccagtat ctaatcaggc
     1381 aatctcaaat ttgcttgata caccaaaaac attaattgtt actcaggaag aactgacacc
     1441 aagagctaaa gacaagagtc caagtgctat tcatgtttct gttgataatt tcttagcgtc
     1501 ccctcgttat gatgaaattg tagcttcatt aacaggagct tctccaatag cagaaattga
     1561 aggagatata ccaacttcag caccagtaga tagtcaggaa attgacctta accatattga
     1621 tgctgtagta gttgcttatg gtaaagcaca gggaactgca actatgggct gtgaaacgat
     1681 tcgggctatc tttagaaaca agaatattcg tattccagtt tctactgcca aaatttcaga
     1741 attaggtgaa tttaattcta aaaacataat gattgtaaca actatttctt tacaggcaga
     1801 agtgcagcaa gcagcaccga attctcaatt tcttattgtg gatagtttag taacaacacc
     1861 agaatatgac aaaatggctg ctagaatgta caaatagaac tagaggtttc taaattacga
     1921 atgctattaa ccaaacgaga agaacaatta ttgaaggctt tcctacatgt agggaagctt
     1981 tcaatgcaag atatgactga aatcttacag gtttcatcta gaacaattta tcgaacttta
     2041 tcagatttga cagatagcat ggagcaatat ggaatcgaaa taacgaagca tgggaaatac
     2101 tatattttga ctggagagtt ggatgatttg ccgacagaac ttgaagtgtt agttgagtat
     2161 agtccccaag aaagacaaga gttgattacc tatcgccttc tgactgagag tggttttgtc
     2221 accaatgaag cattgcaaga gtgcacgaaa gtcagtaatg taactattat tcaggatatt
     2281 tcagatattg ataagcgtct tttagacttt gatctgaaaa ttgaacgaca aaaaggttat
     2341 cggatttctg gtgattcagt tggtaagaga agatttttgg ctattttact gacaaactgt
     2401 atctcagtag cagatttttc aaccggtaat tttgggagct ttgatatttt agaagcagat
     2461 agaactaggc tggccagtca gattgttaat aagcaactgt caggttttcc agatatggat
     2521 gctaggatga agatgttttt tgcgatcttg ttatctctta taggtcagga gcaaaacatt
     2581 gaaaattcac ctaatactag taagcaggct ttggaaattt ctcaaaaaat ttttcaaact
     2641 tactctaagc agactgcaca attttatagt attcaggaaa ttatctattt tgcgagcatc
     2701 ttggatgaat taatcattaa acgtcaggac aatccgctct ttacggagaa atttgatggt
     2761 gaatttttct acaatatttc aaatctgatt gatacggttt ccatgtatac caagattgac
     2821 ttttttaagg acaaggtttt attcaatttt cttttccatc atattcggct cagtttaggc
     2881 gtccctatcc tttttcaggg tgaaaatttg ccagaatctg tccagatttt agttgaaagg
     2941 aataaatttc tttatacagt catcagtctt ttagtgaatg atatttttcc gaaatatctt
     3001 catacagagt atgagtatgg catgattgcc ctacatttta tctctagctt aggccgtagt
     3061 ccagagattt atccagtccg tgttttgctt ttaacggatg aacgtcgggt cactagagat
     3121 ttattagtca gtaaaattaa gagtgttgct ccttttgtag agttgataga tattcagtct
     3181 ctagtagatt accacagtat tgatctcagt cagtatgatt atattttatc taccaagccg
     3241 ctgactaatc aggaaatcga tgtaatttct agttttccaa ccgtcaaaga attgcttgaa
     3301 ttacaggaac gacttcagta tgtacaggca catcgtacaa ttgtcgcgcg tgatgctatc
     3361 gctccagaga aaagttatga cttgcaagat tatttaatat ctagtagtca gcttttgagt
     3421 caattcgagt tggttcaatt ggagaataat caatcatttg agcacacggt agaacaaatc
     3481 atccaatatc agaagaatgt gagtgacaga gattacctaa caagaaaatt gttatctcac
     3541 ttccagaata gtcctatggc tattcctaat actggtctgg tgcttttaca tagtcagtct
     3601 agcaaagtaa caacaaatag ttttactatg tttgaactca aactacctat ctccgcattg
     3661 tcaatgaaac tagaggaaga agaggtcaaa aggtgtctgc taatgctaat gtctaaagaa
     3721 gctagcgagg aagcgagaga tttaatgaca gctattagtc agtcgattat tgaaaatcat
     3781 ctttatacag agatttacaa gacgggaaat caatccatta tttatcagat gctaaatact
     3841 atttttaacg aaaaaattaa gaaattggag aactaatatg aaacttgaaa aacatttgat
     3901 taagcttaat aaacaatttt ctaacaagga ggaagctatt tgttattgtg ggcaagttct
     3961 ttatgagggt ggatatgtta atgaagacta tattgaagcc atgattgagc gagataaaga
     4021 gctatctgtt tacatgggta actttatcgc cataccgcat gggacagatg cagcaaaaaa
     4081 tgatgtcctc aagtctggta ttacagtcgt tcaagtccct agaggggttg attttgggaa
     4141 tgtatctaac cctcaagtgg caacggttct ttttggtatt gctggtattg gtaatgaaca
     4201 cttagaaatt attcagaaaa tttctatctt ctgtgcagat gtagataatg ttcttaaact
     4261 agcagatgct cagtcaaaag aggaagtatt gcgcttattt gatgctgttg aataattgaa
     4321 tttcgtcatt tgtcatctag tatatatgtc cctcaaatag gaaaaggaga aattgaatga
     4381 aacattctgt tcattttggt gccggtaata tcggtcgtgg ttttataggt gaaattctat
     4441 ttaaaaatgg tttccatatt gattttgtgg atgtcaataa tcagataatt catgctctaa
     4501 atgaaaaggg caagtatgaa attgaaattg cacagaaagg acagtctcgt atagaagtaa
     4561 ctaatgtggc tggcattaat agcaaagaac atcctgagca agtcattgaa gcgattcaaa
     4621 agacggatat tattactact gcaatcggac ctaatatact cccttttatc gccgaacttc
     4681 tagccaaagg aatcgaagct cgccgagttg caggaaatac acaggcattg gatgttatgg
     4741 cctgtgaaaa tatgattggc gggtctcaat ttctttatca agaagtcaag aaatatttaa
     4801 gtccggaagg tttgacattt gctgataact acataggttt tccaaatgct gcagtagaca
     4861 ggattgttcc agcacaaagt cacgaagatt ccctttttgt tgtggtcgag ccctttaatg
     4921 aatgggtcgt ggaaaccaag cgtcttaaaa atccagattt acgtctaaaa gatgtgcatt
     4981 atgaagaaga tttagaaccc tttattgagc gaaaactttt ttcagtcaat tctggacatg
     5041 caacttcagc ttacattggt gcgcattatg gtgccaagac aattttggaa gctcttcaaa
     5101 atcctaatat caaatctcgg attgaatctg tattagctga aattcggagt ctcttgattg
     5161 ccaaatggaa ctttgataaa aaagaattgg agaattatca caaagtcatt atagaacggt
     5221 ttgaaaatcc tttcatagtg gacgaggtta gtcgcgtagc tcgtactcca atccgaaaat
     5281 taggctataa tgaacgattc atccggccga tacgtgaatt gaaagaactc agtttgtcat
     5341 ataaaaacct acttaaaaca gttggctatg cctttgacta tcgcgatgta aatgatgaag
     5401 aaagtattcg attaggtgaa ttgttggcta aacaatcagt caaagatgtt gttatacaag
     5461 ttacaggttt agacgaccaa gaattgattg agcaaattgt agagtatatt taatcttttt
     5521 cgaaaatctc ttcaaatcag gttagcatcg ctttgtctta ggcatatgtt gttctatcta
     5581 caacctcaaa gcagtgcttt gagctgactc cgtcagtctt atctgcaatc tcaaaacact
     5641 gtttgagtta tctgcggtaa tctttctagc ttgtctttga tttttgttgt tatttataag
     5701 gtaaaagaag ctggacaaaa agtcttcaaa atcgggaaaa ggcagcctat cgggtgttca
     5761 aaaatcttga taggatgtcc tttattatgg aaagccttat tggattttct cctcagattg
     5821 agtttttgat cagctttatg agataggtct tgctagagat gtagcccatc atgttatttt
     5881 tatggacagt gggaaaattg ttgaaaaaaa taatgcccat caattcttta gtcgtccaag
     5941 agaagaacga accaagcaat tttggaacga attctttcga atgcgatcta tatagtaaaa
     6001 tgaaaca
//
LOCUS       DAVMGZ010000107         5972 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000107 DAVMGZ010000000
VERSION     DAVMGZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.108"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            64..831
                     /locus_tag="VYQ93_001659"
     CDS             64..831
                     /locus_tag="VYQ93_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEV3336313.1"
                     /translation="MIGISLIPALYNIIFLSSMWEPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            916..1596
                     /locus_tag="VYQ93_001660"
     CDS             916..1596
                     /locus_tag="VYQ93_001660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV3336314.1"
                     /translation="MADGSRKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKSRAEINGIIAVLAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMV
                     TTLTTWNSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQT
                     ISINKSFS"
     gene            complement(1697..4249)
                     /locus_tag="VYQ93_001661"
     CDS             complement(1697..4249)
                     /locus_tag="VYQ93_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV3336315.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4311..5933)
                     /locus_tag="VYQ93_001662"
     CDS             complement(4311..5933)
                     /locus_tag="VYQ93_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3336316.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         1997 a          993 c         1109 g         1873 t
ORIGIN      
        1 aaaggagaaa ctatgtttaa agaatggaaa gcaatttaaa aaaaaccaac ctttattatt
       61 gtcatgatcg ggatttctct tattccagct ctgtacaata tcatattttt gtcatcaatg
      121 tgggagccat atgggcaatt gtctgactta cctgtggcag ttgtaaataa tgataaagag
      181 gcttcctata atggtaatac tatggcaata ggaaaagaca tggtgtccaa tttaaaagaa
      241 aataaaacct tggattttca ttttgtagat gaagaggaag gaaagaaggg attggaagat
      301 ggcgattact atatggtagt gactttacca agtgatttat ctgaaaaaac aactacatta
      361 tccaatattc aatcgacagc agcttatcaa tcattgacaa gtgagcaaca aactgagata
      421 agtgattctg tatctcaaaa ttcaactgat agtattcaat cggctcagtc aattgtagct
      481 ttagtacaag atttacaggg aagtttagaa aacttacaaa atcaatcttc taatctttcg
      541 actttaaaaa atcaatctaa tcaagtatca cctattactt ctacttcttt gataggattg
      601 tcaagtggat taacagagat acaaggagat gttactagca aattagttcc tgccagtcag
      661 tcgattgcat caggtgtaaa cgcatatact acaggtgttg ataaagtttc tcagggcgca
      721 agtcaactaa gtgaaaaaaa tgccaccttg acaggtagtt tggataaact agtttcaggc
      781 tcaaacacct tgacacaaaa atcttctaga ttgacagcag gagttggtta attacaatca
      841 ggatctgggc aattagcaga caaatccagt cagttacttt caggtgcttc tccattagag
      901 aatagagcta ataaattggc agatggatct aggaaactag cagaaggtgg aacaaagtta
      961 acttctggat tggaagattt acagacagga cttgcttctt taggacaagg actaggtaat
     1021 gctagtgatc aactcaaatc agtatcaaca gaatctaaaa atgcagagat tttgtcaaat
     1081 ccactcaatc tttcaaaaac agacaatgat caagttcctg taaatggaat cgcaatagct
     1141 ccttatatga tatcagttgc tctttttgtt gcagcaatat caacaaatat gatatttgcg
     1201 aaattgcctt caggacgtca tccagagagc cgttgggctt ggttgaaatc tcgagctgaa
     1261 ataaatggta ttatagctgt tttggcagga attttggtat atggaggagt tcagcttatt
     1321 ggtttaactg ctaatcatga gatgagaata tttattctca tcatcctaac aagtttagta
     1381 ttcatgtcta tggtgaccac tttaacaacg tggaatagcc gtataggagc tttcttctca
     1441 cttattttgc ttttactaca gttagcatca agtgcaggta cttatccact tgctttgaca
     1501 aatgatttct ttagatctat taatccctgg ttaccaatga gctattcagt ttcgggatta
     1561 cgacaaacaa tctccatcaa caagtcattt tcctagctgt catactagct ctatttactg
     1621 gtttaggtat gctagcctat caacataaga aaatggaaga agattaaaaa aatcgaccga
     1681 ttaactggtc gattttttat gccttagatg actttcgtct gtgattatag attccaaata
     1741 gtaagagaga agtaaaggaa cagattgctc cagtaataaa accattggga atgaaggaaa
     1801 gtgtaatagt tcctttcccc ttgggaatgt caactttcat aaatccagtt tgagcttgtt
     1861 taatttctat tttcttacca tcttggtagg cagaccaacc tttgtcataa ggaatggtga
     1921 agaaaataga tgtatcttgt tggacatcat atgtagcaaa aaccttgttt ttagaagttg
     1981 atactgtgac aggttgttct ttaatttttt gaattgcctc ggtgaaagtt ttggtatcta
     2041 aacgatagaa ggtaggagat tcaaatgata cttgtgaatt tccagggaaa ctaacattga
     2101 tattgaaagt ttttttctct ttagtatatc ctagattaaa gaaggagaag acattatcag
     2161 ttgtaaaagt ctttttttca ccatttacaa ggatgtcaac cttcttttgt ttatcgttag
     2221 aaaagtgaag gtttatgaaa gagagataaa cttggctgtt ttctggaact tcaatttgat
     2281 actggattgc tgcatcttca tttgaagaac ttgtgacact aatcaaatca ttagtatttt
     2341 ctattttttc tgttttttca taaggtattg gagaaaaata atcaaaattg acgttagcaa
     2401 gttgatttaa aaatgaggcc tgattatcca aggtatgttc attgaacttg acatcattgt
     2461 aaacagattg actagcaact gcaatcggaa gagagtattg attttcatat agggtaagat
     2521 tatctttttg atagatatct ttaaagccat acttatcaat aggactgtct gagatattgt
     2581 actggatacc aaataaacta tcagccaaaa tactattatt tgcatatcgg agattgagat
     2641 tagtcccaga ggatttaaaa ccaagtttat ctaaagtaga gcttgatgaa cgatttcgaa
     2701 cagatgaaaa ttgagagatt ccattgtagt tgaatttcat actgtcattt cctgtctgag
     2761 tttgtagttt ttcagtacga gtaaattgat ttccaatata tgttgagaaa gattccatag
     2821 ctgggatatc tcgactataa gcacttcgag aagcaaatcc ccattcctta gcaattccgt
     2881 ccatttgaga tgaagcattt aaactcattt caaccagtat aaataaagag attagaatgg
     2941 caaatagatt cacagatata aactttttga taactgcaag gagtaaaaga gaatagacaa
     3001 ccaaaaattc aagagtaagc agaatattca aatctgttaa aaaagaataa tgcgatttta
     3061 gatagatggt agctaaaaat cctgctacta caagaaaaag cgaaactaaa aaattccaga
     3121 ctttaagttc tttcagacgc tttaagactt ctgctgctgt gtaaattaac aaggtagaga
     3181 aaatccaagc atagcgatgt aaaaacatgt ttggagtatg catgccttgc caaaataagt
     3241 caagagcttc tatgtaaaag cttgcaatta gaaatgcaaa gaatattaca tatatgagtt
     3301 tcacgtgaaa cttaatagat ttcagcgtaa aaaataaaat ggtcaaaata aagggaagta
     3361 gtccaacaaa aatcattggg atggccccat actttgttgt gtcaaaggaa ccaatgaatt
     3421 gcttagcaaa gagatcaaga taccagctac tttcagtttg aaactttgta acttcagtca
     3481 atttttcccc atgtgtctgt aaatcaaata gagtgggaag agtcataatc aaactagcca
     3541 taccagctaa aaaggagata actatgaaat caagaacaga tgattttcga gtcttaaagt
     3601 cccacgaaat ttgacagaga taccagaaaa taagaaacaa tactgtcata tatccaaaat
     3661 aataattttg aataaataag attgacagac ttgtaaagta caataggagt ttcttttcag
     3721 ttatcagtag atgtaaacca gttataatta aaggaatcaa gataaaaaca tctagccagg
     3781 tttttatctc taattgactg acagtgaaac tcatcagagc ataggaagta gataaggcta
     3841 gttttaaagt ctgagggata gattgaaaca atttattcaa actaaaaaag gttgacagac
     3901 caatcaatcc aaattttaag agagtggtca gatagacagc atctggcata ttcgttagat
     3961 caaaaaagta aactagaggc gcgagaaaac tacccaagta ataactagat agggcataga
     4021 agtttagccc tagaccactt gtaaaggtgt aaaacagact actatttcca tgtaggatat
     4081 ttcgtaaggc tacatcaaaa ataacgtatt gatgaaagcc atctcctaat agaggagagt
     4141 tgtcgctatt ccagtagata ccttgagata gatatactcc agacataatc actacaggaa
     4201 tgatgaaaga aataaaatag gttcgatatg tttttaaaaa tgatttcatg ttacctcgta
     4261 gaatgataga aaactcagtt ggttaaccca actgagtttt gaagttttat ttagtctttc
     4321 caaagttctt taacttttgc ttgtacttct gcattttcta ggaattcatc gtaggtttca
     4381 tcgatacggt caatgacgcc atttttagat aagacaatga tatggttagc caaagtttga
     4441 ataaactcgt ggtcatggct ggcaaagatg attgattctt taaagttttt caatccatca
     4501 ttcaagcttg agatagattc caagtccaag tgatttgttg gatcatcaag tacaaggaca
     4561 tttgatttta agagcatgag ttttgaaagc atgacacgaa ctttttctcc ccctgacaag
     4621 acatttacag gtttgttaac ttcatctcca gagaagagca tacggccgag gaagccacgt
     4681 aggaaagtat tgtcatcttc ttctttactt gcgaattgac gcaaccagtc aagaattgat
     4741 tctcctcctg caaaatcagc tgagttatct tttggtaggt aagattgact agttgtaact
     4801 ccccacttga cagttccttc atagtcaata tctcccatga ttgcacgaat taatgcagtc
     4861 gtttgaatat cattttgtcc aataagtgct gtcttatcat ctggacgcaa gatgaaacta
     4921 atattatcca agatagtttc accatcaatc tttacagtta gattttctac tgtcaagaga
     4981 tcattaccaa tctcacgttc cgctttaaag ttgataaatg gatatttacg actagatggt
     5041 acaatctctt ctagctcaat cttatcaagc attttcttac gtgatgttgc ctgccttgac
     5101 ttagaagcat tggcagagaa acgagcaacg aattcttgca attgtttaat tttttcttct
     5161 gctttagcat tacggtctgc tagcaattta gcagcaagct cagaagattc cttccagaag
     5221 tcgtagtttc cgacatagag tttgattttt ccaaagtcaa ggtcggccat gtgagtacaa
     5281 actttgttta agaagtgacg gtcgtgggat actacgataa ctgtgttatc aaagtcaatc
     5341 aagaagtctt ctaaccaagt aatcgattgg atatccaaac cgttagtagg ctcgtccaag
     5401 agaagaacat ctggtttacc aaaaagtgct ttggcgagga gaacctttac tttttcaccg
     5461 ttggccaatt cgctcatgtt ttggtagtgt aattcttctg gaatgtttag gttttgaagt
     5521 agttgagagg cttcactctc tgcttcccaa cctccaagtt cggcaaactc tccttcgagt
     5581 tcggcagcac gaaccccgtc ctcgtctgag aaatcttcct tcatgtagat agcatctttc
     5641 tctttcatga tgctataaag tttttcattt cccatgataa cgacatcaat ggcacgttca
     5701 tcttcgtagt caaagtgatt ttgacgaaga acagagagac gttcatctgg accaagagag
     5761 atgtgaccag tagtaggttc gatatctcca gctaaaattt ttaaaaaggt tgattttccg
     5821 gcaccattag caccgattaa tccgtaagta tttccttctg taaatttgat attgacatca
     5881 tcaaaaagtt tgcgatcact aaaacgtagt gaaacatcag atactgtaag caatattttt
     5941 ctcctatatg tgtaatatat ttattctact ag
//
LOCUS       DAVMGZ010000108         5958 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000108 DAVMGZ010000000
VERSION     DAVMGZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.109"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            28..1878
                     /locus_tag="VYQ93_001663"
     CDS             28..1878
                     /locus_tag="VYQ93_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV3336317.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(1964..2176)
                     /locus_tag="VYQ93_001664"
     CDS             complement(1964..2176)
                     /locus_tag="VYQ93_001664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336318.1"
                     /translation="MKIKEQTRKLAAGSSKHCFKVVDGTDEVSSKHCFEVVDGTDEVS
                     SKHCFEVVDRTDEVSNHIRQGDVDVV"
     gene            complement(2226..2858)
                     /locus_tag="VYQ93_001665"
     CDS             complement(2226..2858)
                     /locus_tag="VYQ93_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV3336319.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(2880..3752)
                     /gene="sdaAA"
                     /locus_tag="VYQ93_001666"
     CDS             complement(2880..3752)
                     /gene="sdaAA"
                     /locus_tag="VYQ93_001666"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV3336320.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(3761..4432)
                     /gene="sdaAB"
                     /locus_tag="VYQ93_001667"
     CDS             complement(3761..4432)
                     /gene="sdaAB"
                     /locus_tag="VYQ93_001667"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV3336321.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            4674..5261
                     /locus_tag="VYQ93_001668"
     CDS             4674..5261
                     /locus_tag="VYQ93_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV3336322.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(5314..5634)
                     /locus_tag="VYQ93_001669"
     CDS             complement(5314..5634)
                     /locus_tag="VYQ93_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV3336323.1"
                     /translation="MQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
BASE COUNT         1723 a         1161 c         1287 g         1787 t
ORIGIN      
        1 aactctttta aggaggagta gatttctatg aataaaaaac taacagatta tgtgattgat
       61 ctggtggaaa ttttaaataa acaacaaaag caggttttct ggggaatatt tgatattttc
      121 agtatggtgg tttccatcat tgtatcttat attttatttt atgggctgat taatccagca
      181 cctgttgact acattatcta tacgagtttg gccttcctgt tctatcaatt gatgattggt
      241 ttttgggggt tgaacgcgag cattagtcgt tacagcaaga ttacggattt catgaaaatc
      301 ttttttggtg tgactgctag cagtgtcttg tcatatagta tctgttatgc cttcttgcca
      361 ctcttctcca tccgtttcat cattctcttt atcttgttga gtaccttctt gattttattg
      421 ccacggatta cttggcagtt aatctactcc agacgcaaaa aaggtagtgg tgatggagaa
      481 caccgtcgga ccttcttgat tggtgccggt gatggtgggg ctctttttat ggatagttac
      541 caacatccaa ccagtgaatt agaactggtc ggtattttgg ataaggattc taagaaaaag
      601 ggtcaaaaac ttggtggtat tcctgttttg ggctcttatg acaatctgcc tgaattagcc
      661 aaacgccatc aaatcgagcg tgtcatcgtt gcgattccgt cgctggatcc gtcagaatat
      721 gagcgtatct tgcagatgtg taataagctg ggtgtcaaat gttacaagat gcctaaggtt
      781 gaaactgttg ttcagggcct tcaccaagca ggtactggct tccaaaaaat tgatattacg
      841 gaccttttgg gtcgtcagga aatccgtctt gacgaatcgc gtctgggtgc agaactgaca
      901 ggtaagacca tcttagtcac aggagctgga ggttcaatcg gttctgaaat ctgtcgtcaa
      961 gttagtcgct tcaatcctga acgcattgtc ttgctcggtc atggggaaaa ctcaatctac
     1021 cttgtttatc atgaattgat tcgtaagttc caagggattg attatgtacc tgtgattgcg
     1081 gacattcaag actatgatcg tttgttgcaa gtctttgagc agtacaaacc tgctattgtt
     1141 tatcatgcgg cagcccacaa gcatgttcct atgatggagc gcaatccaaa agaagccttc
     1201 aaaaacaata tccgtggaac ttacaatgtt gctaaggctg ttgatgaagc taaagtgtct
     1261 aagatggtta tgatttcgac agataaggca gtcaatccac caaatgttat gggagcaacc
     1321 aagcgcgtgg cggagttgat tgtcactggc tttaaccaac gtagccaatc aacctactgt
     1381 gcagttcgtt ttgggaatgt tcttggtagc cgtggtagtg tcattccagt ctttgaacgt
     1441 cagattgctg aaggtgggcc tgtaacggtg acagacttcc gcatgacccg ttactttatg
     1501 accattccag aagctagccg tctggttatc catgctggtg cttatgccaa agatggggaa
     1561 gtctttatcc ttgatatggg caaaccagtc aagatttatg acttggccaa gaagatggtg
     1621 cttctaagtg gccacactga aagtgaaatt ccaatcgttg aagttggaat ccgcccaggt
     1681 gaaaaactct acgaagaact cttggtatca accgaactcg ttgataatca agttatggat
     1741 aagattttcg ttggtaaggt taatgtcatg cctttagaat ccatcaatca aaagattgga
     1801 gagttccgca ctctcagtgg agatgagttg aagcaagcta ttatcgcctt tgctaatcaa
     1861 acaacccaca ttgaataaaa aagaaaaacg catagtatca agttacacaa ccttggtaat
     1921 atgcgtttta ttatgtagag acttatactc ttcgaaaatc tcttcaaacc acgtcaacgt
     1981 cgccttgccg tatatggtta ctgacttcgt cagttctatc cacaacctca aaacagtgtt
     2041 ttgagctgac ttcgtcagtt ccatccacaa cctcaaaaca gtgttttgag ctgacttcgt
     2101 cagttccatc tacaacctta aaacagtgtt ttgagctgcc cgcagctagt ttcctagttt
     2161 gctctttgat tttcattgag tattacttca ttttcttctg aaatggaatt gttacccagt
     2221 ctatgctatt gaaaatacgc caaaacttct aagggtttgt gagcgatata atcaggttga
     2281 tagtttagta gatctgcttg ctctccaaat ccccaagtga tggccaattt ctgaatacct
     2341 gtttctcgag ctcccagcat atcaaacttg gtatctccga tgatgatggc ttgttctggt
     2401 gctagttgat gtgtctgcaa ggcttggtga atgacatctg ccttatgggg tgcttcaggg
     2461 ctagaaccat aaatgccatc aaagaaatga tggatttcca agttttttgc catgtcttga
     2521 gcagtagatg tatcctttgt cgtggtgatg tagagtggat aactgctcga taactcctca
     2581 agcaagtcta taatctgagg aaagagttga gcttcataga tgccttttgc cttatagtaa
     2641 gaacgatata tctgcacggc ttcagaaatt tggtctttgg acaggcaggt cgcaaaacta
     2701 ctttcgagag gtggtcccat aaaaccacga atagttttgg catcagggct aggcaccccc
     2761 agctctttaa aggtataggt aaaggcattg tgaatcccga tagaactatc aacgagggtt
     2821 ccatccaaat cgaaaaaaat cgctgtgata gaggtcatgg tttctcctat ttgataagct
     2881 tattctccga aaatttcttt ttggaggcga cgaccagtag gggtggtagc gagtccacct
     2941 tcagctgttt cacgaaaggc agttggcatg cttgctccta cttggtacat ggcatcgatc
     3001 acttcatcca cagggatttt agattcgata cctgccaagg ccatgtctgc tgcgatgaaa
     3061 gcaaagctag ctcccatggc attacgtttg acacagggaa cttcgaccaa acctgcaaca
     3121 gggtcacaga tgaggcctag catattttta atgacaaagg caatagcttg actggcctga
     3181 taaggtgttc cacctgcagc cagagtcaag gcggcagcac tcatagcaga ggctgaacca
     3241 acttcagctt gacacccacc ctcagcacct gagatggagg cattgtttgc gatgactagt
     3301 ccaaaggcac cagcagcaaa gaggaaatcc aattgttgct cgtggctgag gtctaatttt
     3361 tcaatagcag cagtgagaac ggatggcaga cagccagcac ttccagcggt tggagtggca
     3421 cagaccaagc ccattttggc attgtgttca ttgactgcga tggcatttcg ggcagccgag
     3481 agaatcgtat aatctgacag agtttttccg ttttcgatgt agtgatccaa tttggcagca
     3541 tctccacctg tcaggccact acgagattta ttttcattga ggccaagttg gacagaggct
     3601 ttcataactt ccagattgcg ttccatgaga aggaagactt cttcacgttc gcgaccggtc
     3661 aattcaaact ctgttgtaat catgagttct gcgacatttc cttgaaagtc cagatctgct
     3721 tgctcgacca attctttgat agaataaaac atgcttcctc ctatttaaag aaattgacat
     3781 tgtggagatg agggattttt cgaatttctt cgatagcctc atcacagttg cgactgtcaa
     3841 cttcgataat cataatggct ttttcaccag ctttttcacg agtgacattc atctgggcga
     3901 tattgatacc atagcgggaa agcgcctctg taacaagggc aatcatacct ggaatatctt
     3961 gatgaacgat gatgatagtc ggtgtattca tattgagaga gacggcaaaa ccattgagtt
     4021 cggttacctg aatatttcct ccaccgatag aaataccagt cacgctgatg gtcttgtggg
     4081 catttttaac agtaatttta gtggtgttag ggtgaggggc attgctgtct ttctgaatgg
     4141 tccagacaat cttgatacca cgcttgtggg caatttccag actatttgga atttcaggat
     4201 catctgtatc cattcctaaa atacctgcaa caagggctag gtctgttccg tgaccacgat
     4261 aggtcttggc aaatgagtta aaaagttgga attcaacttc tgtcggagta tcatcaaaaa
     4321 tggaagagac aatcttccca atacgaacag caccagcggt atggctacta gatgggccaa
     4381 tcataactgg tccgatgata tcaaagacag attgaaaacg aagtgatttc atcagtttcc
     4441 ccttataaaa attcttatct ctattatatc aaagaatgag gggcttggct ttaattgtgg
     4501 atgaaaacct ttctaatacc tcaaatagca taaaaatagt atcttttatg acaaaaaaca
     4561 ccttatttag ggaaataaaa aataattttg taatatttct acataaaagt gtcaagaaac
     4621 ggtaatattt aaagggtatg atagaactat agaaagaagg agaattttcg aatatgaaat
     4681 caataactaa aaagattaaa gcaactcttg caggagtagc tgccttgttt gcagtatttg
     4741 ctccatcatt tgtatctgct caagaatcat caacttacac tgttaaagaa ggtgatacac
     4801 tttcagaaat cgctgaaact cacaacacaa cagttgaaaa attggcagaa aacaaccaca
     4861 ttgataacat tcatttgatt tatgttgatc aagagttggt tatcgatggc cctgtagcgc
     4921 ctgttgcaac accagcgcca gctacttatg cggcaccagc cgctcaagat gaaactgttt
     4981 cagctccagt agcagaaact ccagtagtaa gtgaaacagt tgtttcaact gtaagcggat
     5041 ctgaagcaga agccaaagaa tggatcgctc aaaaagaatc aggtggtagc tatacagcta
     5101 caaatggacg ttatatcgga cgttaccaat taacagattc atacctgaac ggtgactact
     5161 cagctgaaaa ccaagaacgt gtagcagatg cctacgttgc aggacgttac ggttcatgga
     5221 ctgctgctaa aaacttctgg cttaacaatg gctggtatta agaactaacg aacaaataat
     5281 atgtaaagag tcgaggaaat ccctcgactt tttttataat ttctttggtt gataatgggt
     5341 ctctacttct tttttgagat gagacagcat agaagtttcc tcggtcagct ctaggagtga
     5401 gaaccctttt tggataagtt tcgcatcatc cctacaaatc ccaatacgga catagcagat
     5461 agccagccta tcgtagcttt tcttattctt ggcgtcctcc agtaaagtgt agcagatttg
     5521 cttacacatg tttttatctt gattgtatag atagagtgtg gaaaggttta atagaattgt
     5581 cattcgcaag tcatataaat gttgatagtt tttatagact tctaagcgtt gcaagatttt
     5641 ctcggattat tgacaaataa acggacattc tgatagacga ttgccctagc ggcttagtgt
     5701 aaactttaca aatttggctt gtaatttttt tataaaattc acttcagtta cttgacagtt
     5761 attatatttt cgcttatgct tattatatag ataaaaggac gggataggag tttaaaaatt
     5821 tgaaagcgct atccataatc ataacgcata attttctcct tttcgtgaaa aaagttaacg
     5881 taattttgaa atgagcttaa taagaggtac tatgtaggta gaaaataaag ataggagaaa
     5941 aagtatgaat aaaaaaat
//
LOCUS       DAVMGZ010000109         5719 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000109 DAVMGZ010000000
VERSION     DAVMGZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.110"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(82..2553)
                     /gene="parC"
                     /locus_tag="VYQ93_001670"
     CDS             complement(82..2553)
                     /gene="parC"
                     /locus_tag="VYQ93_001670"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV3336324.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQIGIVPILSSYIAHRREVILARSRFDKEKSEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(2973..4916)
                     /gene="parE"
                     /locus_tag="VYQ93_001671"
     CDS             complement(2973..4916)
                     /gene="parE"
                     /locus_tag="VYQ93_001671"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV3336325.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            5052..5693
                     /gene="plsY"
                     /locus_tag="VYQ93_001672"
     CDS             5052..5693
                     /gene="plsY"
                     /locus_tag="VYQ93_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV3336326.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
BASE COUNT         1526 a         1332 c         1113 g         1748 t
ORIGIN      
        1 ctaaaatagc aagtatattt tgtaattttc agaaaatttc tccaataaaa accagctctt
       61 agaactgatt cttcatttca cttatttatc ttcagtaact acttcctgaa gataagcgtc
      121 aaaaacttct tcatctgaaa tcgtgtcaga aatgaagctt ccattgctag tgcgttctga
      181 caagttcaag tcttgcaatc ggctttcata gattgttcct ttattggatt ggacaagcag
      241 agtttggtcg ttcacatcca cttccgtact gaagaaatcg ccaacaaatc cttgctctgc
      301 aactgctcct gccaagaaga cacgatgcgg tttgtttttc aactcacgca agacttgtaa
      361 tcctcgtttg gcacggctgg ttgctagaat ttcctcaatg gaaacacgtt tcaagcttcc
      421 acgctgggtc aagaggtaga aggacgaagt attacagata aagccagatt ggaggacatc
      481 atcttctttc aaattcatag ccttgacacc tgctgcctta gcaccgacaa ccggaacctc
      541 ttcgatattg aaacgcaggg cataaccatt ttgactaacc aagacaacat catctagttt
      601 aatcggagcc actgctacaa tctgatctgt atcgtctttg agcttagcat acttgacaga
      661 cttagatcta taggtccgcc atggagtgaa ttcttttcgc tctacccgtt tgatttgacc
      721 aaggcgagtc actgcaaagt aggttgtcgc atcgtcaaac tgatccagta cttccacata
      781 aaggatttct tcattcgttt caaagtttgt gatggtttgg ctcagatgct ctccgatgtc
      841 cttccaacga atatctgcca actcatggat tggtctgtag atgacatttc caagacttgt
      901 gaacatcaag aggtgctggg ttgtcttggc agattgaaca aaaatcaaac ggtcatcatc
      961 acgcttgcca atttcttcca aggtggaagc cgcaaaggaa cgtggactgg tacgcttgat
     1021 gtaacctgcc ttggtcacgc tgacgtaggt atcttcctca gcgataagac tagctgtatc
     1081 aatctcaatt gctttcgcag tgtcttctaa agaactcaaa cgaggagttg caaatttctt
     1141 cttgacctca cgaagttctt tcttcatgag attgtacata gtcctttcat caccgataat
     1201 agccgccagc atagcaatct tctcacgaag ctctgcttct tcttcctgca agacaaccac
     1261 atcggtattg gtcaaacggt acagttgcaa agttacgata gcctcagcct gttcttccgt
     1321 aaaatcatag ctaactttga ggttttcctt ggcgtccgcc ttattctcag aagcacggat
     1381 aagagcaatg acttcatcca aaatcgaaat cacacgaatc aaaccttcga cgatatggag
     1441 acgtttctca gacttttctt tgtcaaagcg tgaacgcgcc aaaatcactt ctcgacggtg
     1501 agcgatatag ctagacagga ttggaacaat cccaatctga cgaggtgtga aattgtcaat
     1561 cgccaccata ttaaagttgt agttgatttg taggtcggtg tacttaaata agtagttgag
     1621 aacaagctca gtattagcgt ctttcttaag ttcgatagcg atacgaagac catcacggtc
     1681 agactcatca cgaacctcag caatcccagc taccttgtta ttaacacgaa catcatcgat
     1741 tttcttgact agattggcct tattgatttc ataaggaatc tcagtaataa cgatttgttc
     1801 cttaccacct tttagctttt caatttcagt cttggaacga acaaccacgc gccctttccc
     1861 agtctcataa gctttcttga tttcatcacg accctgaata atagcccctg tagggaagtc
     1921 tggtccaggc aagaattcca tgagtttatc aatctttgca gttgggtggt caatcatgta
     1981 aactgcagca tctatgacct cagctaaatt atggggagga atgtctgtgg cataaccagc
     2041 cgaaatccca gtcgaaccat tgaccaagag gtttggaaag gctgctggca agaccgttgg
     2101 ttctttctcc gtatcgtcaa agttccatgc aaaaggaact gtctttttct cgatatcctg
     2161 aagaaggtag cctgcaattt cagacaaacg tgcctcagta taacgcatag ccgcaggagg
     2221 atctccgtcc atagaaccgt tattaccgtg catttcaact agaatctcac gatttttcca
     2281 gttctgtgac atacgaacca tggcatcata gatagaagaa tccccgtgtg ggtggaaatt
     2341 ccccatgatg ttcccgactg acttggccga cttacggtag ctcttgtcaa aagtattgct
     2401 atccttattc atagaataaa gaatacggcg ttgaaccggc ttcaacccat cacgaatatc
     2461 tggcaaagcc cggtcttgaa taatgtactt ggagtagcga ccaaagcgct ctcccatgat
     2521 gtcctccagg gacatgtttt gaatgttaga cataagatac aaagcccata aaataccaag
     2581 tgaaaataga aaattcttga agtaagcaaa ctcacaagag aatttatctt tttcacacag
     2641 tatctagggc gtgttcaact cctttcaaag aatgtagagt aggtttttat gcagtaaaag
     2701 atattttacg ggaattcctc ccgtgttcag ttacgataag taaccaaact atcctgtttg
     2761 tatttttcaa tatgaaaatc tggttttcca aaattagtct tagtttgtgt cttaaccgct
     2821 cccttaagcg cctctttaag ataagcactc atagcagatt cttcattaat aatcctgcaa
     2881 ttttttcaaa ccaagatttt caaactgctt tttcacatag tcattcacat ccgactctaa
     2941 tttccagttt actaacatat tattttcttt cattaaaaca ctgtcgcttc ttctagcgta
     3001 aacttgacat tatcttcaat ccatttacgg cgtggttcta ccttatctcc catgagaaca
     3061 ttgacgcggc gttcggcgcg cgctaaatct tcaattgtga cacggatgag ggtacgtgtt
     3121 tctgggttca tggttgtttc ccagagctgg tccgcattca tctcaccaag tcctttgtat
     3181 cgttggaggg tagcgccttt accgaactgt ttacggagtt cttctagttc tccatccgtc
     3241 caagcgtagg ccacttcttc tttcttgcct ttacctttgg acatcttgta aagaggtggg
     3301 agggcaatat agacatgacc tgcctcgact agcggacgca tgtaacggta gaaaaatgtc
     3361 aagagcaagg tctggatatg ggcaccgtcg gtatccgcat cggtcatgat aatgatctta
     3421 tcatagttgg catcttcaat agagaagtct gctccaacac ccgcaccaat ggtataaatc
     3481 atggtattga tctcttcatt tttgaggata tccgccatct tggccttggc tgtattgaca
     3541 accttaccac gaagaggtag aatagcctgg aacttgcggt cacgaccttg tttggcagaa
     3601 ccaccggcag agtccccctc aactagatag agttcattct tagcaggatt cttagattgg
     3661 gctggggtca atttcccaga caacaagccc ttatctttct tgtttttctt cccatttcgg
     3721 ctctcatcac gcgccttacg tgctgcttca cgagcatcac gggccttgat agccttgcgg
     3781 atgaggttag aagctaattc cccattttcc ataaggaaaa aggtcaactt atcagccact
     3841 attccatcca caactgggcg agctaggggg cttcctagtt tatccttggt ctgtccttca
     3901 aactgcaagt gttcttcagg aactaagata gaaagaacgg ccgctagtcc ctcacgatag
     3961 tctgaacctt caaggttttt atctttttcc ttgagaagac ctgttttacg tgcatagtca
     4021 ttcatgacct tggtaatggc agacttgagt cctgtctcgt gcgttccacc gtccttggtg
     4081 cgaacgttat tgacaaagga tagaatgtta tctgagaatc cgtcattgta ctggagggct
     4141 acttccactt gaaaaccatt gtcttcccct tcaaagtaaa gaactggcgt caagatttcc
     4201 ttatcttcgt tgagataaga aacaaaatct tgtactccat tctcatagtg gaactcaatc
     4261 gcttcatctg ttcgcttgtc cgttaaagac aaggtcacat ttttcaagag aaaggctgat
     4321 tcattaaggc gctctgaaat ggtattgtac ttgaaatctg tcgtagaaaa gatagtcgcg
     4381 tcaggcataa aagtaacttt ggtgcctgtt ttagacttgg gtgctgtacc gattttcttc
     4441 aaagtcgtga caggttttcc accattttca aaacgttgct tgtaaactgc gccatcacgg
     4501 gtaatttcaa cttctaacca gctagaaagg gcgttaacaa cggaagaacc cactccgtga
     4561 agtccacctg atgtcttata gccaccttga ccgaatttcc ctccggcatg aagaatggta
     4621 aagataacct caacagttgg aattcccata gcgtgcatac ctgtcggcat cccacgtcca
     4681 tggtcttgaa ccgttagact accgtcttta ttgatagtta catcaatacg atcaccaaac
     4741 ccagacaagg cttcatcgac tgcattatca acgatttccc aaactaggtg atgaagacca
     4801 gcgccatcgg tcgatccaat atacatccct ggacgttttc ggaccgcatc caacccttct
     4861 agcacctgaa tagcatcatc attataattg ttaatattga tttccttttt tgacacaagg
     4921 aacctcctat tcgttcatct ttactattct acaggttttc caaggatttt gcaaaatttt
     4981 tctttctccg atgtgacaat tttagcagag attctctgct tttctttccc aattcatgat
     5041 ataataggag tatgattaca atagttttat taatcctagc ctatctgctg ggttcgattc
     5101 catctggtct ctggattgga caagtattct ttcaaatcaa tctacgcgag catggttctg
     5161 gtaacactgg aacgaccaat accttccgca ttttaggtaa gaaagctggt atggcaacct
     5221 ttgtgattga ctttttcaaa ggaaccctag caacgctgct tccgattatt tttcatctac
     5281 aaggcgtttc tcctctcatc tttggacttt tggctgttat cggccatacc ttccctatct
     5341 ttgcaggatt taaaggtggt aaggctgtcg caaccagtgc tggagtaatt ttcggatttg
     5401 cgcctatctt ctgtctctac cttgcgatta tcttctttgg agctctctat cttggcagta
     5461 tgatttcact gtctagtgtc acagcatcga tcgcggctgt tatcggggtt ctgctctttc
     5521 cactttttgg ttttatcctg agtaactatg actctctctt catcgctatt atcttagcac
     5581 ttgctagttt gattatcatt cgtcataagg acaatatagc tcgtatcaaa aataaaactg
     5641 aaaatttggt cccttgggga ttgaacctaa cccatcaaga tcctaaaaaa taaaacgcca
     5701 gttctgtact gcccccaaa
//
LOCUS       DAVMGZ010000110         5578 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000110 DAVMGZ010000000
VERSION     DAVMGZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.111"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(106..2364)
                     /locus_tag="VYQ93_001673"
     CDS             complement(106..2364)
                     /locus_tag="VYQ93_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV3336327.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2593..2823)
                     /locus_tag="VYQ93_001674"
     CDS             complement(2593..2823)
                     /locus_tag="VYQ93_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV3336328.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3013..3696
                     /locus_tag="VYQ93_001675"
     CDS             3013..3696
                     /locus_tag="VYQ93_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3336329.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3696..4430
                     /locus_tag="VYQ93_001676"
     CDS             3696..4430
                     /locus_tag="VYQ93_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3336330.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4584..5441
                     /locus_tag="VYQ93_001677"
     CDS             4584..5441
                     /locus_tag="VYQ93_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV3336331.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
BASE COUNT         1553 a         1048 c         1248 g         1729 t
ORIGIN      
        1 tactatatat cataatggac aaagggcaag ctgtcaagaa attacagtta ttttagtaaa
       61 aaaagaagca gaaaaatttc attttctact ccttttctat aggatttatt tttctttttc
      121 agaactttta acttcagctt ttttggcaga tttaatctga atgtttccct tgctagtcat
      181 aactgctttg agatcttttt cgcttggatt ttcaaggtag tagtcagtga ttgtgtcctc
      241 aatatagtct tgaatagtcc gacgaagtgg gcgtgctccc atttttggat cataacctag
      301 gtcaaccaac ttttccttga ccttgtcagt tacatccaaa cgaatgttgt tgctagagag
      361 gcgcttgtta acatctgcta gcatgagctc gacaatctga aggaggttat ccttgctgag
      421 agccttaaat tcgataatgc catcaaaacg gttcataaac tctgggctaa agaagttacc
      481 gagttcaccg aggacagaat tggtacgtcc ttctctagca gcaccaaatc caacgctagc
      541 ttcggtcttt cctgtacctg catttgaggt catgataatg atggcatcct tgaagctaac
      601 ggtgcgtcct tgcccgtctg tcaaacgacc atcgtccaag acttgaagaa acatgtgcat
      661 aacatctggg tgagcttttt ccacttcatc gagaaggatg agagaatacg gattgtggcg
      721 aactttttca gttaattgac cagcctcatc atagccaaca taacctggag gagcgccgac
      781 caacttagcc acactatgtt tttccatgta ttcactcata tcaaagcgaa tcatactatc
      841 agcagaacca aaaagttcga tagccagttg tttggaaagt tctgtcttac cgacaccagt
      901 tggtccaacg aagaggaagc ttccgattgg gcggttaggg gtaccaagtc cgacacgatt
      961 acggcgaata gccttggcaa tcttatcgac tgcatcatct tggccaataa catgagactt
     1021 gagatcttcg gctagatgga tgagttgaga ttgttctttc tctttcaaat caccaacagg
     1081 gatattggtt ttctgctcga taatgtgctc aatagttttc tcgctgatga taggagtatc
     1141 ctggtctgtg atctttttct tttgcatttc cttatacttg gcaatctggt cgcggaagta
     1201 ggccgccttc tcaaaatctt cttctcgtgt agcttgagac ttgagatttt cagcctcaat
     1261 caagcgctga tcaattactt taggatccac aaaattcaag gtcaagttca tcttagaacc
     1321 agcttcatct aggaggtcaa tggccttgtc aggcaagaag cgatcttgga tgtagcgatt
     1381 ggaaagagtt gcagctgctt caatcgcagc atcggtatat tgaacgtggt ggtaatcttc
     1441 gtatttcttt tgaatccctt tgagaatagt gattgtttca tccaccgttg gttcatcgac
     1501 tttaacaggc tgcatacgac gctcgagggc agcatccttt tcaatgatac ggtattcatt
     1561 gagggtagta gcaccgacta gttgcagttc tccacgagca agggctggct tgaggatatt
     1621 tcctgcgtcc atattaccat cactcgcaga accagcacca acaatttcat ggatttcatc
     1681 gataaagagg atgatgtctt cacgtttgcg aatttcttcc atgagttttt gcatgcgttc
     1741 ttcaaattgt cctcgaatcc ccgttccttg aactaagcta accacatcca gacggatgac
     1801 ttgtttacct tggagtttat gtggcacatc gccatcgaca attttctgag ctagaccttc
     1861 gacaacggcc gtttttccga caccaggttc accgataagg acaggattat tcttggttct
     1921 acgattgaga atctcgatga cacggataat ctcatcgtcg cgcccaataa cggggtcaat
     1981 gtctccacga cgggcaattt cagttacatt aataccaaat tcttccagca ggcctttttc
     2041 ttggctaggt ggcggagttt gagcagatcc acgattttgg gaaccataac cgccgtttcc
     2101 accgtaacct ccacctgatt gggttggggg aataggagga gtattgctag aaggtctgaa
     2161 attgtttaga tcattgaaga aatcaccaaa gggatcgaag tcacgattgt tcagatccgt
     2221 catacctttg aagaggctat tgttaggatc tgttttgata atcttatagc agttttgaca
     2281 gaggtcaatt tgtttttgtt ttccattgag attggtgtaa agatgaattg ttgagtcgtt
     2341 aattttacag ttttgacaaa gcatacatct acctcatttc tttctttagc ctgacttgtt
     2401 taaaggtcaa aaatagtcaa attctatact ctcattataa caggattggg gtgtaaagca
     2461 agtaaaaaga ttgaagcttt gagaagttgt tacaatgaaa atttttgtga atttggacta
     2521 taaaaaaatc ctatttgtga gacaaaaagg attttactta gacatgcagg gacaatccca
     2581 acttgtttta gattagtaga gaagttcgta aatttccatt gcgattaagt caatgctatc
     2641 aaacgtatac gtttcgcgat cttctacgtc acgaagggta aagattgggc cgttttcttc
     2701 gtcgtggctg tacgcaactt ctgcaacaac aactccttcg cgttcaaaat tacgttttaa
     2761 atttccgcca tcgtttgcca ttgcttcaag gcggttgatg attctaacca aatgtgattc
     2821 cattttgatt ctccttcatt tcttatcgtt actattttac cataaaggag ggcaacttga
     2881 ctagaaaaaa ctaagatttc tcgctaattc tgctagttta aagattttta aataaattgg
     2941 ataaaaaatt taaattttaa ttcaaaacat gttataatca tacagtattc tattttagaa
     3001 agcagtgtga ctatgaattt ttctttttta cctaagtatt taccttattt taactatggg
     3061 gctgttgtga cgattcttat ttctatctgt gttatctttt tgggaactat tttgggtgtt
     3121 gtcttggctt ttgggcaacg ttcaaagttt aaaccgcttg tttggttggc caacttgtac
     3181 gtttggattt tccgtgggac accgatgatg gttcaaatta tgattgcctt tgctcttatg
     3241 catatcaatg ctccgactat tcagattgga attttaggtg ttgatttttc gcgtctgatt
     3301 ccagggattt tgattatctc tatgaatagt ggtgcttatg tttcggagac tgttcgtgcc
     3361 ggaatcaatg cggttccaaa aggtcagcta gaggcggctt attcgctagg gattcgtctt
     3421 aaaaatgcga tgcgttatgt gattttgcca caagcagtca aaaatatctt gccagcattg
     3481 gggaacgaat ttatcaccat tatcaaggac agctccctct tatcagctat tggggtcatg
     3541 gagttgtgga atggggctac aacagtttct acaacaacct atctaccttt aacaccactt
     3601 ttatttgcag cattttacta cttgattatg acctctattc tgacagtagc cttgaaagct
     3661 tttgaaaaac atatgggaca aggagataag aaataatgac agaaaccttg ataaaaattg
     3721 aaaatttaca taaatctttt ggaaagaatg aagtattgaa gggcatcaac ctcgagatta
     3781 aaagaggaga agttgtcgtt atcatcggtc cttcagggag tgggaaatct accttgcttc
     3841 gctctatgaa tttgttggag gaagcaacca aggggaagct tatctttgag ggagtcgata
     3901 ttacggacaa gaagaatgac ctgtttgcca tgcgtgagaa gatgggcatg gttttccaac
     3961 aatttaacct ctttcctaat atgactgtga tggaaaatat taccttgtca cctatcaaga
     4021 ctaaaggtga aagcagggaa gttgctgaga agagagccca agagcttttg gaaaaagttg
     4081 gtttgccaga taaggcagac gcttatccac agagtctgtc gggtgggcag caacaacgga
     4141 ttgccatcgc gcgtgggttg gctatggaac cagatgtttt gctctttgac gaaccaactt
     4201 cagccttgga tcctgaaatg gtaggtgagg tattggctgt tatgcaagac cttgccaagt
     4261 cagggatgac tatggttatc gtaacacatg agatgggatt tgcccgtgag gtggcagatc
     4321 gtgttatctt tatggcagac ggtgtggttg ttgaagatgg gacacctgag cagatttttg
     4381 aacaaaccca agaacaacgg actaaagatt tcttgagtaa ggttttataa tctattttaa
     4441 aatttcaaga caagattagt gaaaagttca accagagctt tttcttatag tttgaaacta
     4501 taggataacc taggaaagaa gtgttagagg gcactagcag tttttggtat aatggaagat
     4561 atttgaaaga aaagagaagt gatatgacac agattattga tgggaaagct ttagcggcca
     4621 aattgcaggg gcagttggct gaaaagactg caaaattaaa ggaagaaaca gctctagtgc
     4681 ctggtttggt agtgattttg gttggggata atccagccag ccaagtctac gttcgcaaca
     4741 aggagaggtc agctcttgcg gctggttccc gtagcgaagt agtgcgagtt ccagagacca
     4801 ttactcaaga ggaattgtta gacctgattg ctaaatacaa tcaggatcca gcttggcatg
     4861 ggattttggt tcagttgcca ttaccaaaac acattgatga agaggcggtt ctattggcta
     4921 ttgacccaga aaaggatgtg gatggtttcc atcctctaaa tatgggacgc ctttggtctg
     4981 gacatccagt catgattcca tcaacacctg caggaattat ggaaatgttt catgaatatg
     5041 ggattgaatt agaaggtaaa aatgcggttg tcatcggtcg ttctaatatc gttggaaaac
     5101 ccatggctca gcttcttttg gccaagaatg caacagtgac tttgacccac tcacgcaccc
     5161 ataatcttgc taaggtggct gctaaagcag atattctggt ggtcgcaatc ggtcgtgcca
     5221 agtttgtgac tgctgacttt gtcaaaccag gtgcggtagt cattgacgtt gggatgaatc
     5281 gtgatgaaaa tggtaagctc tgtggggatg tggattatga ggcagttgcc ccacttgcta
     5341 gctatattac gccagtccct ggaggtgtcg gccctatgac cattactatg ctgatggagc
     5401 aaacttatca agcagcactt cggacattgg atagaaaata agagaaaaat cgctgaagag
     5461 agtgtatttt ctatagctat atttaaaatg gcagaaatga atattaaatt ttagatataa
     5521 gattacaaaa ggaggtctgc gcctcctttt tgttgtataa tggagtgagg tgattaga
//
LOCUS       DAVMGZ010000111         5508 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000111 DAVMGZ010000000
VERSION     DAVMGZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.112"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            142..825
                     /gene="rpiA"
                     /locus_tag="VYQ93_001678"
     CDS             142..825
                     /gene="rpiA"
                     /locus_tag="VYQ93_001678"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV3336332.1"
                     /translation="MENLKKMAGIKAAEFVKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQIIAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRRFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            839..2050
                     /locus_tag="VYQ93_001679"
     CDS             839..2050
                     /locus_tag="VYQ93_001679"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV3336333.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            2052..2585
                     /locus_tag="VYQ93_001680"
                     /pseudo
     CDS             2052..2585
                     /locus_tag="VYQ93_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285096.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            2609..3418
                     /locus_tag="VYQ93_001681"
     CDS             2609..3418
                     /locus_tag="VYQ93_001681"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV3336334.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            3894..4289
                     /locus_tag="VYQ93_001682"
     CDS             3894..4289
                     /locus_tag="VYQ93_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEV3336335.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            4299..4874
                     /locus_tag="VYQ93_001683"
     CDS             4299..4874
                     /locus_tag="VYQ93_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV3336336.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            4899..5411
                     /locus_tag="VYQ93_001684"
     CDS             4899..5411
                     /locus_tag="VYQ93_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV3336337.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNFPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         1691 a          974 c         1276 g         1567 t
ORIGIN      
        1 taaagtgatt tgtcagagtt ctgcttttat taaaaaaatt taatttcaag gataaataag
       61 cagtattcta aaggtacttt tagatgaaat aaaagccttt acatggtata atagaggtag
      121 ctctttaatg gaggtgtttg agtggaaaat ctgaagaaaa tggcaggtat caaggctgct
      181 gagtttgtca aggatggaat ggtcgttgga cttggaacag gctcgactgc ctattatttt
      241 gtcgaagaaa tcggtcgtcg tatcaaggaa gaaggattgc agattatagc tgtaacgact
      301 tctagtgtga ccactaaaca ggcagaaggc cttaatatcc cactcaaatc tattgaccaa
      361 gtagactttg tcgatgtgac agttgatggc gcagatgaag tggatagtca gtttaatgga
      421 atcaaaggtg gtggtggtgc tcttctgatg gagaaggttg ttgcgacacc atcaaaagaa
      481 tacatttggg tggtggatga aagcaagctg gtcgaaaaac taggtgcttt taaattgcca
      541 gtagaagtgg ttcagtatgg tgcagaacag gtctttcgtc gttttgaacg agctggctac
      601 aaaccaagtt tccgtgaaaa agacggccaa cgttttgtga cggatatgca gaattttatc
      661 attgacctcg ccttggatgt cattgaaaat ccaattgctt ttggacaaga attggaccat
      721 gtcgttggtg ttgtggagca tggtttattc aaccaaatgg tggataaggt catcgttgct
      781 ggacgggatg gagttcagat ttcaacttca aaaaaaggaa aatagaaggg ggcataagat
      841 gtctaaattt aatcgtattc atttggtggt actggattct gtaggaatcg gtgcagcacc
      901 agatgctaat aactttgtca atgcaggggt tccagatgga gcttctgaca cactgggaca
      961 catttcaaaa acagttggtt tgaatgtccc aaacatggct aaaataggtc ttggaaatat
     1021 tcctcgtgaa actcctctta agactgtagc agctgaaagc aatccaactg gatatgcaac
     1081 aaaattagag gaagtatctc ttggtaagga tactatgact ggacactggg aaatcatggg
     1141 actcaacatt actgagcctt tcgatacttt ctggaacgga ttcccagaag aaatcctgac
     1201 aaaaatcgaa gaattctcag gacgcaaggt tattcgtgaa gccaacaaac cttattcagg
     1261 aacggctgtt atcgatgatt ttggaccacg tcagatggaa actggagagt tgattatcta
     1321 tacttcagct gaccctgttt tgcagattgc tgcccacgaa gacattattc ctttggatga
     1381 attgtaccgt atctgtgaat acgctcgttc gattaccctt gagcgtcctg cccttcttgg
     1441 tcgcatcatt gctcgccctt atgtaggtga accaggtaac ttcactcgta cggcaaaccg
     1501 tcgtgacttg gctgtatctc cattttcccc aactgttttg gataaattga atgaggctgg
     1561 tatcgatact tatgctgtgg gtaaaatcaa cgatatcttt aacggtgctg gtatcaacca
     1621 tgacatgggt cacaacaagt caaatagtca tggaattgat acactattga agactatggg
     1681 acttgctgag tttgaaaaag gattctcatt cacaaaccta gttgactttg atgcccttta
     1741 cggccatcgt cgtaatgctc acggttaccg tgattgcttg catgagtttg atgaacgctt
     1801 acctgaaatt atcgcagcta tgagagagaa tgaccttctc ttgattactg cggaccatgg
     1861 aaatgaccca acgtatgcag gaacggatca cactcgggaa tatattccat tgttggccta
     1921 tagccctgcc tttaaaggaa atggtctcat tccagtagga cattttgcag atatttcagc
     1981 gactgttgcc gataactttg gtgtggaaac tgctatgatt ggggaaagtt tcttagataa
     2041 attggtataa gatgacgcgc tatgctttgc tggtgagagg tatcaatgtt ggtggtaaga
     2101 ataaggtcgt catggcggag cttcgtcaag aattgacaaa cttgggactg gaaaaggttg
     2161 agagctacat caacagtggc aatattttct ttacttcgat agattccaaa gcccaattgg
     2221 ttgaaaagct agagactttc tttgcagtcc attatccatt tattcagagc ttttctttac
     2281 tgagtctaga ggactttgag gcggaacttg aaaatctacc agcttggtgg agcagagatt
     2341 tggcacgaaa agattttctc ttttacactg agggtttgga tgtggaccaa gtcatcgcga
     2401 cagttgaaag tttagagctg aaagatgaag tgctttattt tggaaaactt gggattttct
     2461 aggggaaatt ttctgaagaa tcctattcta agactgccta tcataagtac ttgctgaggt
     2521 gcctttctac cgccacatta ctattcgtaa tgctaaaacc tttgacaaaa ttggtcaaat
     2581 gctaaaaaaa taataaagga gacacacaat gacattttta aacaaaatcc atgaaactgc
     2641 tactttcctg aaagaaaagg gaattgcagc ccctgagttc ggtctaatcc ttgggtcagg
     2701 acttggagaa ttggcagaag aaatcgaaaa tccagttgta gtagactatg ctgagattcc
     2761 aaactggggc cgttcaacag tagtcggtca tgctggtaaa ttggtatatg gtgaactggc
     2821 aggtcgcaag gtcttggctc ttcaagggcg tttccatttc tatgaaggga atcctctgga
     2881 agtggtgact ttcccagttc gtgtgatgaa agttcttgga tgtgaaggtg ttattgtaac
     2941 caatgcagct ggcggtatcg gatttggtcc tggtaccttg atggctatct cagaccatat
     3001 caacatgacg gggcaaaatc cattgatggg tgaaaacttg gatgactttg gcccacgttt
     3061 cccagatatg tctagggcct acacaccaga ataccgtgct actgcccatg aagtggctaa
     3121 aaaacttaat atcaagcttg atgaaggtgt ctatatcgga gttactggtc cgacttatga
     3181 aacaccagca gaaattcgtt cctataagac actgggagca gatgcagttg gtatgtctac
     3241 ggttcctgaa gttatcgtgg cagcccactc tggcttgaaa gttctgggaa tttcatgtat
     3301 cactaacttt gcggctggtt tccaagaaga actcaatcac gaagaagttg tagaagtgac
     3361 tgaacgtgtt aaaggtgatt ttaaaggctt gcttaaagcg attcttgctg aattgtaaga
     3421 aaaaagattt aaaaggggga gtgcctctgt cttttcagga ttgactgcct atccgtatta
     3481 aagaagaaac agaggaatac tatgagcttc ttcctgctct tataactgaa agaagcggaa
     3541 gaataggtgt gtctgatctg atagccagca ttgtgaaaga caagattcta ggatactagc
     3601 attagcttcc tagccaagca gactagtatg ataaggagag atgagaatga attgactttc
     3661 tgaatttctc agtcttatca tatatagcac aatgagattt cgcttgagtc tgcttgtaaa
     3721 taaacgaaaa gaaagataag aaataatgaa aattggtcaa cgaattatgc gctttggcat
     3781 aaaaaattaa gtatcggagt tgtatctgtt gtagtcggct ttgatttcta gctccagctg
     3841 gaatttcagc caatgaagta aagcaagatg taacaactga agtggtaata ggtgtgctag
     3901 attctaagga ggaattgaaa gagtcagaaa atgatgctcc aaaactagaa actcctctta
     3961 gagaggagcc aagactagct cctcaaacgc ttccggaagc aagtgaagtt cttgaaaaca
     4021 aaagggaaga gtcaaaagta gagataacag agccagctca agcggatgac atccgcaagg
     4081 ttgttgggga attagccaag gatataagta ttactaagtt gtatatgaca ggtcattctc
     4141 ttggaggcta cctagctcag attgcagcgg ttgaagatta ccaaaaatat cctgattttt
     4201 ataaccatgt attgaggaaa gtgacaactt tcagtgctcc taaagtgatt acttccagaa
     4261 ctgtttggga tgctaagaat ggtttctgag atgttggttt ggaaagtcgt aaattagctg
     4321 ttagtggaaa aattaagcat tatgtggttg ataatgacaa tgttgtgact cccttgattc
     4381 ataataatcg tgatattgtt acatttacag gtaattcacg ctttaaacac cgttctcgtg
     4441 gctattttga aagtccaatg aatgatattc ctaactttaa tattggtaaa caagctacct
     4501 tggataaaca tggttatcgt gatccgaaat tggataaagt gcgattcttt aagaaacagg
     4561 ctctacctca atcttctagt caaccaagcg ctgaaccaat ggaaaatatt gccttaggaa
     4621 aacaggttac tcaaagttcg acagctttcg gaggagatgc tagaagagct gtggatggca
     4681 aagtcgatgg taactatggt cacaattctg tcactcatac aaacttccaa tctaagcctt
     4741 ggtggcaagt agatttggct aaagaagaaa ccattcgcca aatcaatatt tacaaccgaa
     4801 cagacactgc ccaggataga ttggcaaact ttgatgtcat tcttttagac agttctggta
     4861 aagaaattga gtgaaaacgt ataatatctc ctaaagatgt gtcagcacaa attacgatta
     4921 accataaaaa agcgcgctat gttcggattg ggctagaagg ctataatgcc ctcagtcttg
     4981 cagaagttga agttttccgc tttatagcta cgaatgctga aacggcgaca caagtttcta
     5041 agccagttca accaatcagt cagactcctg tgaaagataa aacattgaca attcaacaca
     5101 gtggagctta cattgcccgc tactccataa cttgggaaga agttccagta gataaagatg
     5161 gaaaccaagt tgttcgtagt cattcttggg aaggaaacgg ttgcaaccag actgcaggtt
     5221 ttgtcctcaa cttcccaatc aaagaaaata tgagaaatct gcgagttaag attgagaaaa
     5281 agacgggcct actatggaat agatggcaaa caatctatga aaacagacca attttagctc
     5341 aaccccaccg taaaattacc cattggggta cgacattgaa ttccaaggtg agtgacgatg
     5401 atgtcttgta atctgatggt acaatgacag ttagtttgtc tagtttataa gaaagtacta
     5461 tctgagcttg aatagaactc aggtagctct ctgtgaaaga acaaaatt
//
LOCUS       DAVMGZ010000112         5301 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000112 DAVMGZ010000000
VERSION     DAVMGZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.113"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(308..1870)
                     /gene="guaA"
                     /locus_tag="VYQ93_001685"
     CDS             complement(308..1870)
                     /gene="guaA"
                     /locus_tag="VYQ93_001685"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV3336338.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIRDIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2012..2710
                     /locus_tag="VYQ93_001686"
     CDS             2012..2710
                     /locus_tag="VYQ93_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV3336339.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERHVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2753..3649)
                     /locus_tag="VYQ93_001687"
     CDS             complement(2753..3649)
                     /locus_tag="VYQ93_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV3336340.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSAFTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3658..4593)
                     /locus_tag="VYQ93_001688"
     CDS             complement(3658..4593)
                     /locus_tag="VYQ93_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV3336341.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGSVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLSQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4590..>5301)
                     /locus_tag="VYQ93_001689"
     CDS             complement(4590..>5301)
                     /locus_tag="VYQ93_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV3336342.1"
                     /translation="IVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLGDQTGL
                     EKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQNGYAFE
                     IFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLVSISLSKFEISMELHLPTDIESFLE
                     SSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYFIPKSE
                     KFFLCKDRNNVELWFEEV"
BASE COUNT         1653 a         1150 c          974 g         1524 t
ORIGIN      
        1 gcattaaaat atcctccaat agttttactt tactaatagt atacaggagg agctccatga
       61 atgatagata ccttgttatg acgcgcttac catattagaa agttggctct ctaattaaat
      121 taattctcat tatatatatc aaaagaccgc agtttgaaaa ctacggtcta attttttgtc
      181 caaatgtaaa ccacttggaa ctattattca atttattctt tttagcaaat gttcccgttt
      241 taatcccagt tacagttttt aatcctttgt aaccgttgat tttacaaagc ttttaatttt
      301 tttgcgatta ctcccactca actgttgcag gtggtttact tgtaatatcg tagacgatac
      361 ggttaacatg atccacttca tttacgatac gtactgagat tttttgaagt acttcccatg
      421 gaattttggc aaaatcagca gtcataccat cgatagaagt gatagcacgg attgcaatcg
      481 tgtagtcata cgtacgaccg tcacccataa caccgactga acgaacgcct gtgttaacag
      541 tgaagtattg ccaaatatcg cggtcaagtc cagctttagc gatttcttca cgaagaatag
      601 cgtctgattc acgaacggtt tcaagtttct cttcagtgat ttcacccatg acacggatag
      661 caagtcctgg tcctgggaat ggttggcgcc atacgatatg gtctggcata ccaagctctg
      721 taccaagagc acgaacttca tccttgtaaa gagtattgag tggttcaatc aattcaaact
      781 gcatatcttc tggaagacca cccacgttgt ggtgtgactt gatagtttga gctgtatccg
      841 taccagactc gataacatct gtatataaag taccttgagc aaggaatttc acatctttga
      901 gcttgcttgc ttcgtcatcg aatacataga caaactcgtt accgatgatt ttacgttttt
      961 gttcaggatc agaaacgcca gcaagtttgt caaggaaacg tttagcagcg tctgctttga
     1021 cgatattcaa accaaactta ccaccgagca tgtccataac ttgatcagct tcgcctttac
     1081 gaagaagacc gtggtccacg aagatacaga tcaattgatc gccaatcgct ttttggagaa
     1141 gaaccccaac gacagatgag tcaacaccac ctgatagacc aagaaggaca cgtttatcac
     1201 cgacggtttc acgaattttt ttgatctgca tgtcaatgaa attatccatt gaccagtcac
     1261 ctttagcctt acaaatgtta agggcaaagt tacgaaggat atcatttccg tatacagaat
     1321 gacgaacttc tgggtggaat tggataccgt aaatgtgttt atctgggttt tcgatggctg
     1381 cgtatgggca gtcagctgat gtacctgtac gaacaaagtc agcaggaatc tcagtaaccg
     1441 catcaccatg gctcatcaaa acagtctgtt catcaggtgt tgattcaaaa agcgctgatg
     1501 gtgtgtgagt tagggttgat tgaccgtatt cacgatttcc agcatcacct gcaggaacaa
     1561 cttttcctcc aagtttatgg gtcaataact gcataccata acagattccc aaaattggaa
     1621 ttccgagttc gaagatttct gggtcaatat caaatgaacc atcttcatat acagaatttg
     1681 gaccacctga tagaataatt cctacaggat tgacttcacg aacttcagca gctgaaattt
     1741 tatggctttt tagttctgaa aaaacaccaa tatcacggat acggcgtgaa atcagctggt
     1801 tgtactggct accatagtcc aatacgatga ttttttctac atcttgcaaa tcagttgaaa
     1861 tgttgctcat ctttttcctt tctcaaaaga aatatctttt ctcaatattc tatcacaaat
     1921 aagggcgaat taccaactaa acaaggcgaa gtttgacttt ttctagttta ttggggtaca
     1981 atagagaaaa gatagaaatg aagtgaaaaa tatgctacct gcttatatga aaatccatga
     2041 tcagataaaa aaggatattg acgagcaccg ttgggctatt ggtgagaggc ttcccagtga
     2101 aagagattta gctgagcagt ttgcggtcag tcgcatgacc ctccgccaag ccgtatctct
     2161 attagtcgaa gaaggcgtct tagagcgcca tgtaggaagc ggcacctttg tttccagtac
     2221 tcgagtacaa gaaaagatgc gagggacaac cagttttact gaaattgtca aatcccaagg
     2281 taaagttccc tctagccagc tcatttccta caaaaaaacc attcccaatg agcaagaagt
     2341 tgccaagcta ggaatttttc caacggacaa tattatccga atggagcggg tccgctatgc
     2401 cgaccaagtt cccctagttt atgaagttgc ttctattcct gaaaaattca ttaaggactt
     2461 taaaaaagaa gaaatcacca gtcatttctt ccaaaccttg caaaaacatg gctatcgtat
     2521 cggcaaatct caacagacca tctatgctcg ccttgctaaa gaaaagattg cccactattt
     2581 ggaagttgaa aaaggacatg ctattcttgg attgacccag gtttcctacc tagaagatgg
     2641 tactgctttt gaatacgtaa aaagtcaata tgtaggcgaa cgctttgaat tttatcttga
     2701 aaataattag attaccacgc aaaaactctc tgtcacggac aaagagtctt ttttattttt
     2761 ctaagaataa atctttcaaa gaaatcaaag ttacagcaaa caatatcatt gccataccaa
     2821 gaaaatcaat gggataaaat tgttcattca ttattaagaa ggcaaagaaa atcgccgata
     2881 ttggctcaat tgaagccaac aagcttgact tgaccggtcc tatcagactg gctcctttaa
     2941 ggaaagctgt ataggcaaag acagtcccga taaggataat gcctgcaaac gcaaggagaa
     3001 aatcaagact agtcgggata tcggcctgta gaacccctgt aaaaggaaga gcgaccaaac
     3061 ctgctatgac cattcccaca ccaatgacca agctgctccc ccacttttta atcaaggcta
     3121 tgggtaaaat gatatacaga gcataagtaa aggcagaaaa gagaccccag aacagaccag
     3181 caggtgtcat ggataactgg tccaactgcc catgtgttgc gatcaggaag gttcctccga
     3241 tggcgaatat gatggaaact atctctccca gtgtcggtgc caccctatcc ttgatacagc
     3301 tataaattaa aattccgaca ggacaaacat actgaagcac cgtcgctgtt cccgcattgg
     3361 tctcctgaat agcagacaga taggcgaatt ggttgagaaa aagaccaatc agagcaaaaa
     3421 taagaagaga cagcaaactc tttctatcct ttaaaaagac cagtatttta tcctttgcag
     3481 tagcataagc caagagcatg agaattccac cagcgattaa aagacgcaag ttggtcaaga
     3541 ccagagccga aattccgtgt gccattaggt attggccact cgttcctgac aacccccaag
     3601 caataccagc cacaactgtt agtaaagtcc cttttaaact atttgacatg tctctcctta
     3661 ctcttctttg cgaatcaagt tcatcacttg attgcggtct aatatccatt gagctctctc
     3721 gtccttttca taggtacgat tcgataggaa aatagtggct tcttgcttct gacgattcca
     3781 catgataaag gtacctgtat agcccgtatg gtctagccaa tctccttcca aattccatgc
     3841 taaagaacgt tccttgtcat ccaaaggaga aaaattttga cttaagtctc ttgcaaaatc
     3901 atctgctaaa tagtgttcta aaaagatttg taaatccttt atagtcgaaa ataaaccagc
     3961 actcccagca tgtctaccca ggagacgagc cttgggatca tgcactatgc ctgcctctac
     4021 acctctaact gttggaacag caagctcaac tgacccaaac ttggtttccg tcattcccca
     4081 aggtttccag acttgatcct ttaaaatcac atccaaatct tgattaaaaa ttctttccaa
     4141 aataaagccc aacagcaaaa aatggacatc cgaataaaga aaggctggct gacttcgtct
     4201 gttgagatga aacatcgctt cctttaattc aggggctgtt aaaagatcac gattaggaat
     4261 aaaaggatca aggtctgttg catgagtcaa gagctggcga atagtgatgt ctggataatc
     4321 actctcaggt aaaaaatcta ttaccagtct atcaatatct aattgaccta tttcccacaa
     4381 gaaggtacaa actgtgccaa ccccaacaac cttgctgaca ctagctaggt cataaactag
     4441 tcctgcctca gtctgcaagc catgctctgg gtcactctgg cctaaataga actctgtcca
     4501 ttgattgtcc ttaaaatacg caaaagaggc tccgggataa attcctgcct cgatttgttc
     4561 ttctattttt ttaataatct tggtccactt catacttctt caaaccacaa ttcaacatta
     4621 tttctatctt tacaaaggaa gaatttttca gacttaggaa taaaatattc agtagactca
     4681 aacttctggc gaagacttgc tatgtctaat tcattgacca agaacttgag catagataag
     4741 tcccaggtaa ccgtattgtc cacagtcaaa tcctgcccct gagctggaat aaaatcaagg
     4801 gatgccccaa tttcagatga ttccaagaaa ctttcgatat cagttgggag atgtaattcc
     4861 atagaaatct caaatttact taaagaaatt gataccaaat ctgtttgaaa ttcaggcttt
     4921 tctcctactt ctactagact tgctctgtca tcttccgcat gaatcaaaat caaatcatct
     4981 tctggtgaga aaatttcaaa agcgtagcca ttttgacctt tatataatcg atgaatcgaa
     5041 tctgttttag ataagattcc ttcaatttct aagggatttt ccaccttgac aatcaatcta
     5101 gctagttttt ttcttccctc taccttacga gtacgcatac tgggagcttc ttctaaaacc
     5161 agcttttcaa gacccgtttg gtcacctagt gacagaaagg ccgattcttc taacaaggcc
     5221 ttcattccaa gggtttcaat ataaaatgtt tcatttaatt ttctattatt agcttttaaa
     5281 gtaggaataa tccgtacaat c
//
LOCUS       DAVMGZ010000113         5251 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000113 DAVMGZ010000000
VERSION     DAVMGZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.114"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(436..1164)
                     /locus_tag="VYQ93_001690"
     CDS             complement(436..1164)
                     /locus_tag="VYQ93_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV3336343.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1322..2731
                     /locus_tag="VYQ93_001691"
     CDS             1322..2731
                     /locus_tag="VYQ93_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV3336344.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2749..4044
                     /locus_tag="VYQ93_001692"
     CDS             2749..4044
                     /locus_tag="VYQ93_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV3336345.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVNRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            4049..4357
                     /locus_tag="VYQ93_001693"
     CDS             4049..4357
                     /locus_tag="VYQ93_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3336346.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4354..4662
                     /locus_tag="VYQ93_001694"
     CDS             4354..4662
                     /locus_tag="VYQ93_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV3336347.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1779 a          877 c          944 g         1651 t
ORIGIN      
        1 aaaaaataaa atttttttaa atgattatga ttcagaattt attcaatata ttaaaacaat
       61 atttgaacaa ataaattggg agattgaaat tcatttctag caatttgtta gaagtcacag
      121 tctactattc taacctcagt atactatatg tgttacaaga aaagactcaa aaacttctcg
      181 tcagtgtaag aatatttgaa acactattta aaactaaata actataagaa gtagatcagg
      241 tgaacaattt cagtacctac tctataagct atacgaagat atttttcaac atgatggata
      301 aaacgatcac tcttttaaag ccatcttatt tagaaaagag aaaccattta agcatagatg
      361 ggaccttctt gccctgtttt aaatttttct taaactagag tctgtaaaag attggcttca
      421 ttttcacatt ttctcctagg aggaatgtcg taatgcaaaa gaattaaatt caaataaatc
      481 atagcgatga cgactaatcg agtactcaaa cggagttccg ttatccaaat aagccacttg
      541 ttctacttca aataccggtt ccgttggaag gagctgcaag taatgttgct cattttctga
      601 actagaagaa gctcgtaaga tttttgtagc actggcaata tgcaatccta actttccttc
      661 aatgtaattg taaatcgatt tttgtaaaat atcttcagta atacctggaa taacatcggt
      721 actcatataa gtttgttcca gaacataagg tttaccgtca ataatacgca ggcggtaaat
      781 attataaact ggactctgca aagcgacctg tagtttttct gctaaaaatt catttgcaaa
      841 ttctaatttg aagtgaataa tcctcgagtc aattgtagaa tgagcatctt cagatatttt
      901 tgtcagtccc cggatatctc tttctggaac gattaatttt ctttttgagc tgccacgaga
      961 gagaacaaag gttccctgcc cacgttttct aaaaattaag ccctcagaaa ctaacaagtc
     1021 taaagctttt ttgatcgtca ttcgactaca ggcaaactgt ttagctaatg tcatttcatc
     1081 aggtaattgt tcgttcatgg tatatttatt acaaagaatc ttttcacgaa tctcattgta
     1141 aataaataaa tattttgtac tcatttaatc tggttctctc tataatattc taatagtagt
     1201 ataacaaagt tcaagaaatg aggcaaacac aagatatgta tcaacaattt ttaaaaatgt
     1261 ctagacaaat tttaaaatta tgctatactt tatgtataga ataatagaaa ggaggttgag
     1321 catgaaaaaa aagttagtat ttcctaatct gttttggtgg ggagcagctt ctagcggacc
     1381 tcagacagaa ggtcaatatg gaaaagtaca tgaaaatgtg atggactact ggttcaaaac
     1441 gcatccagaa gattttttcg ataatgtcgg acctcttgta gccagtaact tttttcatac
     1501 ttacaccgaa gatttccact tgatgaagga aattggagtt aattctttcc gcacttccat
     1561 ccaatggagt cgactcatca agaatttaga gacaggtgag cctgatccaa aaggtattgc
     1621 tttctacaat gccatcattg aagaagctaa aaagaaccag atggatcttg tgatgaattt
     1681 acatcatttt gatttaccag tggaacttct tcaaaaatac ggtggttggg aaagcaaaca
     1741 tgtagtggag ttattcgtga agtttgccaa gactgctttc acatgctttg gagataaggt
     1801 tcattactgg acaactttca atgagccaat ggtcattcca gaagcaggat acttatatgc
     1861 tttccattat ccaaatctaa aaggaaaggg aaaagaggcc gtacaagtca tctataatct
     1921 aaaccttgct agtgcaaaag tgattcaact atatcgctca ttagaacttg atggaaagat
     1981 tgggattatt ttaaacttga cacctgctta tccaagaagt aattctccag aagacttaga
     2041 agcaagtcga tttacagatg acttctttaa caaagtcttc ttgaatccag ctgttaaagg
     2101 aactttccca gaaagattgg taaaacagct agagagagat ggcgtgttat ggagtcatac
     2161 cgaaaaagag cttcaactga tgaaatcaaa tacggttgat tttcttggag taaactacta
     2221 ccatccaaaa cgtgttcaag cacaagcaaa tcctgaggaa tatcagacgc cctggatgcc
     2281 agaccaatac ttcaaagagt atgaatggcc ggagcgtcgc atgaatccat atcgtggttg
     2341 ggaaattttt ccgaaatcca tttatgatat tgctatgatt gtgaaggaag aatatggtaa
     2401 tatcccatgg tttatcagtg aaaacggaat gggtgttgaa aacgaagcac ggtttatcga
     2461 tgaaaatgga gttatcgatg acgtgtatcg tattgaattt tatgaagaac atttaagatg
     2521 gctacataaa gccattgaag agggaagtca ctgttttgga taccacgctt ggaccgcatt
     2581 tgattgctgg tcttggaata atgcatataa gaatcgttac ggatttatct ccgttgattt
     2641 agaaacgcaa aagagaacca tcaagagctc aggaagatgg tatcgcaaag taagtgacaa
     2701 taacggtttt gaagtagaaa ttgaggagta aagagaggag atattcatat gaaaaactca
     2761 aaatttatag accaatttgc cacctttgct ggtaaactag ggaaccaaat tcatttaaaa
     2821 accctaagag atgcattcgt aacagtaatg ccattatata ttttggcagg tttaatcgtt
     2881 cttttgaaca acacggtatt taagtggatt ttccaagggg atacattaac aagattccaa
     2941 tattggggaa taacaattgc aaacggtact ttaagtattt caggtatgat tattgctgta
     3001 atggttggtt atttcttagc taaaaacaga gatttcgaaa acccgttagc agcatcaatg
     3061 ctatcattag tttctttaat tgtgatgatg ccaaatacag tttctgtagt tcctgacgga
     3121 gcaaaagatg ctgtaaacat ttcaggtgtt ctttcattca acaacacagg tacaggcgca
     3181 atgttcgccg gggttatcgt agcgattatt gcaacagaat tattcattga attatcaaat
     3241 gttaaagctt tacaaatgaa ccttggtgaa aatattccac cagctgttaa tagatcattt
     3301 agcgtattac ttccagtcat gaccgtcatc tccttatttg gggttgtttc agcattatta
     3361 ttcaatataa ctggaatgaa cttaatctca atcattacaa tctttattca agaaccaatt
     3421 cgtcatattg gtacaagctt aatcggggtc attattattt actctttagg aaatatgtta
     3481 tggctatttg gtattcacca agcagttatt tacagtgcca tcctagaacc attactatta
     3541 attaacatta ctgaaaacat cactgcagca aataatggac aagccattcc acacatcatc
     3601 aacctatcac aaatacaaac attcgcttta atgggtggta gtggatctac attatgttta
     3661 ttaatagcaa cattcttagt gagtcgcaat gctgtctcta aaaacgtggc taaattatct
     3721 tttggacctg gtatcttcaa tatcaatgaa ccagtattat tcggttaccc aatcgtttat
     3781 aacatttcat tagctattcc atttatcaca gttccagtcc ttggtatttt aatcagctac
     3841 ttagcaacag ttacaggatt catgagtcct gcatttatac aagttccttg gactacacca
     3901 gtattcttaa atgcatggtt agcaacagca ggggacgtga gagcagttct agttcaattc
     3961 atcatctttg cacttggagt tcttctatac attccattta tcaaagttaa tgacaaagtt
     4021 gttgaacaag aaatggaagg ttaagattat gaaacaaatt ttattagtat gtaatgcggg
     4081 aatgtcgaca agtatgcttg ttaaaaaaat gcaacaaagt gcgactgaac gtgggattga
     4141 aatctctatc caagcaaaat caatgacaga agctaaaaag aatatccatg aagcagatgt
     4201 cattctaatt gggccacaaa ttcgttatga gttacttgct gtgaaagaaa ttgcaggaaa
     4261 tatccctgta gataccattg atatgcggga ttacggaatg atgaatggag caaaagttct
     4321 ggaacaagca cttgcatgga taggagaaat aagatgacag aagaaatgga atatatctgt
     4381 tttcagctca ttgccaatag tggagcagcc aaatcatcct ttattgaagc catccaacta
     4441 gctaaggcag gaaatttaaa agaagcgaaa attaaagttg aagaagcgga agactcctta
     4501 gtagaagcgc acaagattca ctctaatctc atacaaaaag aagctactgg agaaaaaata
     4561 ggattctcct tgctatttat gcatgcagaa gaccaaatgg cttcaacaga gattattcag
     4621 ttgctatcta aagaattcat cgatctctat caaaataaat aggaacactg tcattcccat
     4681 ctgtctttat taatgacgac tacattgtag gaacctccaa aagttggaca aaatgattga
     4741 aaggattgga tcctgcatta cacaagatcc aatccttttc ttttactcta gtttttataa
     4801 tatgttgaat ctagaatagt ccactgtagt ttctaaaaaa ttgctggaaa tggatgttaa
     4861 gctccaattt atttgtttat atcttatttc agtttactat actagcttat tattctagtg
     4921 aaaaattgaa tgagccacta tttatttaat atgtatgatg agggaatgac tcaaaaactt
     4981 cttgtcgata taagaatatc tgagatagta ttttaaaacg aaaggactat aaaagctcat
     5041 ttccttcgca cttattttat agagatctat cattatactc tttagaaatc aaaaagcaac
     5101 acaccttgtc atcgttttat cgtaaatctg ttaatgaggg ggctagtctt atctgcaatc
     5161 tcaaatcagt gttttgagct gaatggcact cgttttctag tttgctcttt gattttaatt
     5221 gggccttagt aaatactact cttaaattaa a
//
LOCUS       DAVMGZ010000114         5160 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000114 DAVMGZ010000000
VERSION     DAVMGZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.115"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            52..1047
                     /locus_tag="VYQ93_001695"
     CDS             52..1047
                     /locus_tag="VYQ93_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV3336348.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVIAKGVNVGISTIYYWIHRGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRPEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIHR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLNYKSPREDFLMANLNLKFSVRNKSRN"
     gene            1073..1717
                     /locus_tag="VYQ93_001696"
     CDS             1073..1717
                     /locus_tag="VYQ93_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV3336349.1"
                     /translation="MLRAGVLDANNGIISIAGVVIGVASTTTNIWIIFLSGFAAILAG
                     AFSMAGREYVSVSTPKDTEEAAVLREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     ANKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSEYR
                     IPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTRGVTFLLEQLFSI"
     gene            complement(1759..3747)
                     /locus_tag="VYQ93_001697"
     CDS             complement(1759..3747)
                     /locus_tag="VYQ93_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3336350.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3749..4507)
                     /locus_tag="VYQ93_001698"
     CDS             complement(3749..4507)
                     /locus_tag="VYQ93_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336351.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPTRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSATLLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQLFQEISDTLTVMASEVN"
     gene            4935..5126
                     /locus_tag="VYQ93_001699"
     CDS             4935..5126
                     /locus_tag="VYQ93_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336352.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         1606 a         1026 c         1022 g         1506 t
ORIGIN      
        1 acctctgtgt tatacttgtt gttcaaccac aaacacaaga aaggcacaga gatgcaagac
       61 aattatacta caaaagccaa acatttgaca atcgatagcc gtcgcttaat cgaaagatgg
      121 aaaaaagaag ggaaatcaaa tagagaaatt gcctctctac ttggaaaagc tcctcaaact
      181 atccacactg aaatcaagcg tgggacagtc cgacaatgtc ttggaaaagg gcgcttcaaa
      241 gaggtttatt ctgccgacta tgctcaacag tcttatgaaa acaatcgcaa gcgctcggtc
      301 aagaaatcaa gcttgaccaa ggaactaaag gaaaagattc tccactatca taaccaaaaa
      361 ttttcgcctg aaatgatggt tattgctaaa ggggttaacg tgggaatttc aaccatttac
      421 tattggattc atcgtggaaa attggggtta agcaagcagg atctgcttta ccctagaaaa
      481 ggaaaagcgc ttaagaaaca ggctagcacc aactttaaac ctgctggtca atccatcgaa
      541 cagcggcctg aagctatcaa tcttcgcttg gagaatgggc attatgagat tgatacggtt
      601 ctacttacga gagcgaaaaa ctactgcttg cttgtcttga cggatcgaaa gagtagacat
      661 cagatcatcc gattgattcc aaataaaagt gctgaggtgg tcaatcaggc tctaaaactc
      721 atcttaaaac aacacaagat tctttccatc acggcagata atggaacgga attcaatcgc
      781 ttgtttgatg tattttctga ggagcacatc tattatgcgc acccctatgc ctcttgggaa
      841 aggggaacta atgagaatca caacaggctc attcatagat ggttacctaa gggaaccaag
      901 aaaatgactc ccaaagaagt cgcattcatc gaaaagtgga ttaacaacta tcctaaaaaa
      961 tgcttgaact acaagtcacc cagagaagac ttcttgatgg ctaacttgaa cttgaaattt
     1021 agcgtcagaa acaaatcacg aaattgattc aaattttgca ggtcgtttaa atatcctgcg
     1081 tgcgggtgtt cttgatgcta acaatggaat tatttccatt gctggtgtgg ttatcggagt
     1141 tgccagtacc acgaccaata tctggattat ctttttatca ggttttgcgg ctatcttagc
     1201 tggtgccttt tcaatggctg gtagagaata tgtatccgtt tcaactccaa aagataccga
     1261 ggaagctgcc gttttgcgag aaaaactctt gctagaccaa gatagggaac tagccaaaaa
     1321 atccctctat gctgcttata tccaaaatgg agaatgcaaa acttctgccc aactcttggc
     1381 taataagatc tttcttaaaa atccactcaa ggctctggta gaggaaaaat atgggattga
     1441 gtatgaagaa tttaccaatc cttggcacgc tgccatttct agcttcgttg cctttttcct
     1501 tagaagtttg cctccaatgc tgtcagtgac catattccca agtgaatatc gcatccctgc
     1561 tactgtcctt attgtcggtg tggcccttct tctcactggt tacactagtg ccagacttgg
     1621 aaaggatccg actagaacag ctatgattcg gaaccttgct attggtctct tgaccagggg
     1681 agttaccttc ctgctcgaac aacttttcag catttagaat acaagaaata cctcgatttt
     1741 gaagtcgagg tatctttttt acatttgcac aatcttgcga taacttcttg aagtaatcat
     1801 aaaaatcagc acataggcga tgaggaagat agcgcagata gacaaggtca caatcaacat
     1861 catagtcgta tccagtacac caatcacttt taaaatcagg ctaagcatat ggtaggcaaa
     1921 ggcgagatgt atgaaggcaa agagcaaagg aaggaagaaa acagttaaaa cctgtttgtt
     1981 gatggtttgc ttgatttgct tttggtccaa accgactttc tgcaagataa taaagcgttc
     2041 acggtcttcg tagccttcag aaatttgttt gtagtagatg accagaacag ttccgaccat
     2101 aaagataatg gataggaaaa taccgataaa gaagacacca ccaaagaggg cactcatctg
     2161 agaactagca tctgctagat tgctaccata aacatagcta ccttctgtgt ctaattgagc
     2221 attaaattga ttgaggtagt tttcatactc ctcagcgacc ttgagttgtt cttcttcgct
     2281 ggcatctaca ttcataccac cgtaaaactg attatagata gccgaatctg ggaattgatc
     2341 caaaaaggct tgtaaatcag gaacaacaag gtaattgtaa tcagtagtca agatattaaa
     2401 cttatttgga acatggttga caatgaaatc tttattaaat tcttctttga cagaaaattg
     2461 atgatcattt agagttagag ctttctgtcc tttcagtccg tcatttttgg caaagagacc
     2521 gacctcattt cctgatagag acagtttttg accagtcata ttttcataat ctttttggtc
     2581 aaataccatg aaaactgttt ttggttggac acggttttgt cctttttcaa aaatagttaa
     2641 cttggttcct tcttgatttg caataccaaa gttactgtaa cgaagcactt ctttctcttt
     2701 aatcttataa cctttgtcac ttgcaaactg gctcaagagt ttgtccaaat cttctttttc
     2761 aacattttgc cctgaaaccc caaaatcatg aggatttaga acttttttaa aggattctgc
     2821 ggaattgaaa atgcttgtcg ctgctgacat ggttaccaaa accattgttg acaaaatagc
     2881 gatagttgct agtccaactg catttttctt catacggaaa atcaagttag aaacagatat
     2941 gagattatta ggttggtaat agtatttctt atttttcttt aagatttgaa ggaaaacggt
     3001 aatccctgca ttgaacaaaa gataagtccc aaagataacc agtaaaacag ctatgaagaa
     3061 ggttgttaag gctgtaagag gatcttttac cgtaagggca agataatatc caatccctaa
     3121 acttatggaa ccaagaatag tttggagagg aaggaagcga ccttttttct ctccgctagc
     3181 tttctcacgt gagagctgga gggcattcat acgggcgatt cgaagggcat tcaggaacat
     3241 gaggcctagg aaaatcaaac cgaagacaag aagtactgta atgacaactt tcatctggaa
     3301 ggtagcgacc agttccacct tcaatttcat cagtttgagt aggaaagcga aaatcaactt
     3361 gtcaaacaag gctccaatac cgattcctgc tccaacagtt agaatcccaa atagcactaa
     3421 ctccttaaag gtcatactga ttagatggcg cttctccaag cctaacatgc catatatacc
     3481 cagttccttg gaacggtttt tcatgacaaa actattggca tagaggacga taatggctga
     3541 cgcaagggta acgacaaaca taccaaatcc aagtgttgct tgaatggtgg ttcctccacg
     3601 gatttccgca atcttgggat tgaaggttag ggagtaaaag agataggtga tggtgactgc
     3661 caagagaaca gccagtgcaa agggatagta gagtttgcgg tttttaatca agttcgatac
     3721 cgctaactta ttggttaatc gaaacatact aattcacctc gcttgccatg acagtcaagg
     3781 tatcagagat ttcttggaac aactgacgct ctgtcttctc tccacggtag atttggttgt
     3841 aaagaatgcc gtctttgata aagagaacac gcttggccct gctagcagct gctgttgagt
     3901 gggttaccat gaggatggtt tgcccacgct cattgatttc attaaagaca tcaagtaagg
     3961 ttgcagatga cttggaatca agggctcctg ttggctcgtc cgcaaggaga atttcaggtt
     4021 ctgtgatgat ggcgcgggct actgctacac gctgtttctg gccaccagaa atctcgtaag
     4081 ggtacttctc ttgcaattgg ttaataccca gattctcagc tgtcaccacc aatttcttca
     4141 tcatctccgt tataggtctt cttgacaaga caagcggaag caagatattg tccttaacag
     4201 acagagtatc tagcaagtta aagtcttgga agacaaatcc tagcttttca cgccggaaac
     4261 tagaagcctg tgaattttta atagttgcgg tgtcagttcc attcaagtaa acctgcccac
     4321 gagttggttt atccaacata gctagaatat tgagaagagt tgatttacca gaaccagact
     4381 cacccatgat ggcaacgtag tcaccctttt ctacggtaaa gtgaatatcc ttgagggctt
     4441 ctacttggtt gccctgaaaa cgtgttttat aaattttttg aacgtgtttt acatctaaaa
     4501 gtgtcatgag aatctccttt cttaatatcc catcaaatga aatgtagctt gcatcatagt
     4561 gcatcccagc tgaacgcgct caatttcttt gtgacttggt tttctagttt tcttatgtaa
     4621 gatttgtttc atgatgttac tcctttgttc tttatgagtc tagtttacat caaaaaaaga
     4681 ccgcttgcca taacctaacc ttacaaaaga gactttaatc ttacattttt gtaagattgg
     4741 gtgaaatata agcaaaatta cttagaaaat gatttgattt cctattttta aagttgtaaa
     4801 atataagtgt gaaaaggctt actttgaaaa ggaggaagcg ggaactaaca ttttagtaag
     4861 cttacctaga taacatacca ttcaatttaa cacttgcaag tcgttcaacc tataaggaaa
     4921 gaaggtgcgc tttaatgaaa cacaaagaac atatactcat cggacttctc tatctgctct
     4981 ctccattcat cggtcagctc ttagttgaac acacacactt tatcagtaca gaatttaccg
     5041 gcactgctta tgttatttgc tggttatcag ttgttatcag catccaccat ttttccaaaa
     5101 atgtattgtc tcaacagcaa aaatagatta aaaaactgcg tttaaattac tgcttttata
//
LOCUS       DAVMGZ010000115         4937 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000115 DAVMGZ010000000
VERSION     DAVMGZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.116"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            313..822
                     /locus_tag="VYQ93_001700"
     CDS             313..822
                     /locus_tag="VYQ93_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV3336353.1"
                     /translation="MNSRVEFRIFTILDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQEFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCMSLFREELSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            986..2020
                     /gene="hrcA"
                     /locus_tag="VYQ93_001701"
     CDS             986..2020
                     /gene="hrcA"
                     /locus_tag="VYQ93_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV3336354.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            2047..2571
                     /gene="grpE"
                     /locus_tag="VYQ93_001702"
     CDS             2047..2571
                     /gene="grpE"
                     /locus_tag="VYQ93_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV3336355.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            3051..4874
                     /gene="dnaK"
                     /locus_tag="VYQ93_001703"
     CDS             3051..4874
                     /gene="dnaK"
                     /locus_tag="VYQ93_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV3336356.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKTKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
BASE COUNT         1567 a          930 c         1094 g         1346 t
ORIGIN      
        1 cgttcaaaaa ctggcgtaaa ttttgctata atattttagt ttaaataaag attttttatg
       61 ccaaacggat tttaacaatg cggatgcttc ctattttgct tctcttttta gctctactac
      121 cggttttctc aaagtttgtc actggaggta gttctttcag ttgaggagta tttttgattt
      181 tcataataga ttgtgtttta ttgatagttt ttgcgattca gatttcttat attttttgga
      241 gattgagtca aaagtggaaa gaattatcta ataaataacg ccaggcatgt tttctaattt
      301 tggggtaaga caatgaatag cagagtagaa tttcggattt tcaccattct tgatttggac
      361 aaggaagaag aatatttaca tgagatgcat ttgaaaggtt ggaggtatag aacgagtcgt
      421 tttggtttgt tctattttga ccaatgtcaa ccagacgatg tcatctaccg tatctatgat
      481 tctagatttc ttaaaaaata taagcatgaa ctgcaagaat ttagagatag aggttgggaa
      541 ttgataggag caggttcttg ttcgattctt cgtaaatcgt cttctgattt acttccagag
      601 gatcaagtct atatgagtaa ggggctcaaa tgggaagtta tgcgatctag acttcgttcc
      661 tgtacagcta ctttcttagg tggtcttgtt gtttgtatga gtttgtttag agaggaactt
      721 tctatgtctt tctttcttat ttttgtttta tatgcttttc tgatttctta tctaattcat
      781 ggttatttca gactaaaaag gaaataccga gtagatgaat agcaaggttc taggtcttca
      841 gattgatttt tagcactctt gataaaagag tgctaatttt ttgagttttt gtcttgacat
      901 tctcttctaa gggtgtataa tagaatcatg agttagcact tggatgcatt gagtgctaat
      961 tgatcagaca gagaggagtg atgagatggt tacagagcgt cagcaggata ttttaaatct
     1021 gattattgac atctttacca aaacgcacga acctgtcgga tcaaaagcct tgcaagagtc
     1081 tattaactct agcagtgcaa ccattcgtaa tgacatggcg gaactagaaa aacaagggtt
     1141 gcttgagaag gctcatactt caagcggtcg gatgccaagt gttgctggtt ttcagtacta
     1201 tgtgaaacac tcactggatt ttgaccgact ggctgaaaat gaggtatatg agattgtcaa
     1261 agcctttgat caggaattct tcaaattgga ggatattctg caagaggctg ctaacttgct
     1321 aacagacctg agtggctgta cggtagtggc actggatgtt gagccgagca ggcaacgttt
     1381 gacagccttt gatatcgttg ttttggggca acatacagcc ttggcggtat ttaccctaga
     1441 cgagtcgcga acggttacta gtcagtttct gattccaagg aacttcttgc aggaggattt
     1501 gctgaaactg aagagcatca ttcaggaacg tttcctcggt cacaccgttt tagatattca
     1561 ctacaagatt cggacggaga ttccgcagat tatccagcgt tactttacaa caacggataa
     1621 tgtcatcgat ctctttgaac acatctttaa ggaaatgttc aacgaaaaca ttgtgatggc
     1681 gggcaaggtc aatctcttga attttgccaa tctagcagcc tatcagttct ttgaccaacc
     1741 gcaaaaggtg gccttggaga ttcgtgaggg gttgcgtgag gatcagatgc aaaatgttcg
     1801 tgttgcagac ggtcaagagt cctgtttagc tgacctagcg gtgattagta gtaagttcct
     1861 cattccttat cggggagttg gaattctagc cattatcggt ccagttaatc tggattacca
     1921 acagctaatc aatcaaatca atgtggtcaa ccgtgttttg accatgaagt tgacagattt
     1981 ttaccgctac ctcagcagta atcattacga agtacattaa gattgaaatc attaaaggag
     2041 gcgaacatgg cccaagatat aaaaaatgaa gaagtagaag aagttcaaga agaggaagtt
     2101 gtggaaacag ctgaagaaac aactcctgaa aagtctgagt tggacttggc aaatgaacgt
     2161 gcagatgagt tcgaaaacaa atatcttcgc gctcatgcag aaatgcaaaa tatccaacgc
     2221 cgtgccaatg aagaacgtca aaacttgcaa cgttatcgta gccaggactt ggcaaaagca
     2281 atcttaccat ctcttgacaa ccttgagcgt gcacttgcag ttgaaggttt gacagatgat
     2341 gtgaagaagg gcttggggat ggtgcaagaa agcttgattc acgctttgaa agaagaagga
     2401 attgaagaaa tcgcagcaga tggcgaattt gaccataact accatatggc catccaaact
     2461 ctcccagcag acgatgaaca cccagtagat accatcgctc aagtctttca aaaaggctac
     2521 aaactccatg accgcatcct acgcccagca atggtagtgg tgtataacta agatataaag
     2581 cccgtaaaaa gctcgcagta aaaataggag attgacgaag tgttcgatga acacaagaaa
     2641 atctatcttt tttactcaga gcttagggcg tgttcgattc ggcaattctg acggtagcta
     2701 aagcaactcg tcagaaaacg gcaatcgcta tggcgtttgc ctagcttcct tactaactcg
     2761 tcgtcgaaat aaaatcgatt tcgactcctc gtgtcgcaat ttacataata gaaaacttgt
     2821 ccgaaacgac aataaactat gaagaaagat aaaatatgtt tggctttgta atagtgagcg
     2881 aagcgaacca aacacgatac tcttcgccgt ggcgctattt gcgcaaattt tgagacctta
     2941 ggctcaaagt ttagtcaaag agattgacga agtcaagctc tgacggcgtc gccacttaag
     3001 aagagtatca aaaagaaaaa tagaaaatta actaacaagg agaaaaacac atgtctaaaa
     3061 ttatcggtat tgacttaggt acaacaaact cagcagttgc agttcttgaa ggaactgaaa
     3121 gcaaaatcat cgcaaaccca gaaggaaacc gcacaactcc atctgtagtc tcattcaaaa
     3181 acggagaaat catcgttggt gatgctgcaa aacgtcaagc agttacaaac ccagatacag
     3241 ttatctctat caaatctaag atgggaactt ctgaaaaagt ttctgcaaat ggaaaagaat
     3301 acactccaca agaaatctca gctatgatcc ttcaatactt gaaaggctac gctgaagact
     3361 accttggtga gaaagtaacc aaagctgtta tcacagttcc ggcttacttc aacgacgctc
     3421 aacgtcaagc aacaaaagac gctggtaaaa ttgctggtct tgaagtagaa cgtattgtta
     3481 acgaaccaac tgcagcagct cttgcttatg gtttggacaa gactgacaaa gaagaaaaaa
     3541 tcttggtatt tgaccttggt ggtggtacat tcgacgtctc tatccttgaa ttgggtgacg
     3601 gtgtcttcga cgtattgtca actgcagggg acaacaaact tggtggtgac gactttgacc
     3661 aaaaaatcat tgaccacttg gtagcagaat tcaagaaaga aaacggtatc gacttgtcta
     3721 ctgacaagat ggcaatgcaa cgtttgaaag atgcggctga aaaagcgaag aaagaccttt
     3781 ctggtgtaac ttcaacacaa atcagcttgc catttatcac tgcaggtgag gctggacctc
     3841 ttcacttgga aatgactttg actcgtgcga aatttgacga tttgactcgt gaccttgttg
     3901 aacgtacaaa agttccagtt cgtcaagccc tttcagatgc aggtttgagc ttgtcagaaa
     3961 tcgacgaagt tatccttgtt ggtggttcaa ctcgtatccc tgccgttgtt gaagctgtta
     4021 aagctgaaac tggtaaagaa ccaaacaaat cagtaaaccc tgatgaagta gttgctatgg
     4081 gtgcggctat ccaaggtggt gtgattactg gtgatgtcaa ggacgttgtc cttcttgatg
     4141 taacgccatt gtcacttggt atcgaaacaa tgggtggagt atttacaaaa cttatcgatc
     4201 gcaacactac aatcccaaca tctaaatcac aagtcttctc aacagcagca gacaaccaac
     4261 cagccgttga tatccacgtt cttcaaggtg aacgcccaat ggcagcagat aacaagactc
     4321 ttggacgctt ccaattgact gatatcccag ctgcacctcg tggaattcct caaatcgaag
     4381 taacatttga catcgacaag aacggtatcg tgtctgttaa ggccaaagac cttggaactc
     4441 aaaaagaaca aactattgtc atccaatcga actcaggttt gactgacgaa gaaatcgacc
     4501 gcatgatgaa agatgcagaa gcaaacgctg aagccgataa gaaacgtaaa gaagaagtag
     4561 accttcgtaa tgaagtagac caagcaatct ttgcgactga aaagacaatc aaggaaactg
     4621 aaggtaaagg cttcgacgca gaacgtgacg ctgcccaagc tgcccttgat gaccttaaga
     4681 aagctcaaga agacaacaac ttggacgaca tgaaaacaaa acttgaagca ttgaacgaaa
     4741 aagctcaagg acttgctgtt aaactctacg aacaagccgc agcagcgcaa caagctcaag
     4801 aaggagcaga aggcgcacaa gcaacaggga acgcaggcga tgacgtcgta gacggagagt
     4861 ttacggaaaa gtaagatgag tgtattggat gaagagtatc taaaaaatac acgaaaagtt
     4921 tataatgatt tttgtaa
//
LOCUS       DAVMGZ010000116         4932 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000116 DAVMGZ010000000
VERSION     DAVMGZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.117"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            217..480
                     /locus_tag="VYQ93_001704"
     CDS             217..480
                     /locus_tag="VYQ93_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV3336357.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            486..2219
                     /gene="ptsP"
                     /locus_tag="VYQ93_001705"
     CDS             486..2219
                     /gene="ptsP"
                     /locus_tag="VYQ93_001705"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV3336358.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            2474..4924
                     /gene="phtA"
                     /locus_tag="VYQ93_001706"
     CDS             2474..4924
                     /gene="phtA"
                     /locus_tag="VYQ93_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV3336359.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
BASE COUNT         1667 a          933 c         1039 g         1293 t
ORIGIN      
        1 tgaaatagca atgattatcc tataaattgt ttttgaagaa actatatcct gtgttttgtt
       61 atctaggata gaagattttc agcaagttat ctgaaaggaa attgaaggaa aatccagaaa
      121 atgcttgaaa aaaatcctag aagatggtat aatactaaat tgtaagggtt atcacatata
      181 actcaaaaaa agaaagaaca aaaggagagt caaactatgg cttctaaaga tttccacgta
      241 gtggcagaaa caggtattca cgcacgtcca gcaacattgt tggtacaaac tgctagcaaa
      301 tttgcttcag atatcactct tgagtacaaa ggtaaatcag ttaaccttaa atcaattatg
      361 ggtgttatga gtcttggtgt tggccaaggt gctgacgtaa ctatctcagc tgaaggtgca
      421 gatgcagatg acgctatcgc tgcaatctca gaaacaatgg aaaaagaagg attggcataa
      481 gggaaatgac agaaatgctt aaaggaatcg cagcatctga cggtgttgca gttgcaaaag
      541 catatctact cgttcagccg gatttgtcat ttgagactat tacagtcgaa gatacaaacg
      601 cagaagaagc tcgccttgat gccgctctac aggcatcaca agacgagctt tctgttattc
      661 gcgagaaagc agtaggtacg ctcggtgaag aagcagctca agtttttgat gctcacttaa
      721 tggttcttgc tgacccagaa atgatcagcc aaatcaagga aactatccgt gcgaagaaag
      781 tgaatgcaga agcaggtctg aaagaagtta cagatatgtt tatcactatc tttgaaggca
      841 tggaagacaa cccatacatg caagaacgcg cagcggatat ccgcgacgtg acaaaacgtg
      901 tattggcaaa ccttcttggt aaaaaattgc caaacccagc ttctatcaat gaagaagtga
      961 ttgtgattgc gcatgacttg acaccatctg atacagctca attggacaaa aactttgtaa
     1021 aagcttttgt aaccaacatt ggtggacgta caagccactc agctatcatg gcacgtacac
     1081 ttgaaattgc tgctgtatta ggtacaaata acatcactga aatcgttaaa gacggtgaca
     1141 tccttgctgt taacgggatc actggagaag tgattatcaa cccaacagat gaacaagcgg
     1201 cagaatttaa agcagctggt gaagcctatg cgaaacaaaa agctgaatgg gcacttttga
     1261 aagatgctca aacagtgact gctgacggta aacacttcga gttggctgcc aatattggta
     1321 ctccaaaaga cgttgaaggt gttaacaaca acggtgcaga agctgttgga ctttaccgta
     1381 cagagttctt gtacatggat tctcaagact tcccaactga agatgagcag tatgaagcat
     1441 acaaggctgt tcttgaagga atgaacggta aacctgttgt cgttcgtaca atggatatcg
     1501 gtggagataa ggaacttcct tacttcgata tgcctcacga aatgaaccca ttccttggat
     1561 tccgtgctct tcgtatctct atctctgaga ctggagatgc tatgttccgc acacaaatcc
     1621 gtgctcttct tcgtgcgtct gttcacggtc aattgcgtat catgttccca atggttgcgc
     1681 tcttgaaaga attccgtgca gcgaaagcag tctttgatga agaaaaagca aaccttcttg
     1741 ctgaaggtgt tgcagttgcg gataacatcc aagttggtat catgatcgag attcctgcag
     1801 cggctatgct tgcagaccaa tttgctaaag aagttgactt cttctcaatt ggtacaaacg
     1861 acttgatcca atatacaatg gcagcagacc gtatgaacga acaagtttca tacctttacc
     1921 aaccatacaa cccatcaatc ctacgcttga ttaacaatgt gatcaaagca gctcacgctg
     1981 aaggtaaatg ggctggtatg tgtggtgaga tggctggtga ccaacaagct gttccacttc
     2041 ttgtcggaat gggcttggat gagttctcta tgtcagcaac atctgtactt cgtacacgca
     2101 gcttgatgaa gaaactcgac acagctaaga tggaagagta cgcaaaccgt gcccttacag
     2161 aatgctcaac aatggaagaa gttcttgaac ttcaaaaaga atacgttaat tttgattaat
     2221 cgaaaagtcc ctgcaactca gttacaggga ctttttttga tattttaaaa agaattttca
     2281 agaaaatctt tcttataaaa agtccaacct tgaaaaagta gtggtcagaa caaaaaatac
     2341 ttaaatggtt cataaaattc ttgacaagtt ggagatttag gagtaaacta ttaaccagtt
     2401 aagtaataga gaggagtttc tgcaatttag aaatgaattg caactagaaa tatcaaatag
     2461 aaagagagtt tcgatgaaaa ttaataagaa ataccttgtt ggttctgcgg cagctttgat
     2521 tttaagtgtt tgttcttacg agttgggact gtatcaagct agaacggtta aggaaaataa
     2581 tcgtgtttcc tatatagatg gaaaacaagc gacgcaaaaa acggagaatt tgactcctga
     2641 tgaggttagc aagcgtgaag gaatcaatgc tgagcaaatc gtcatcaaga taacagacca
     2701 aggctatgtc acttcacatg gcgaccacta tcattattac aatggtaagg ttccttatga
     2761 tgcgattttc agtgaagaat tactcatgaa agatccaaac tataagctaa aagatgagga
     2821 tattgttaat gaggtcaagg gtggatatgt tatcaaggta gatggaaaat actatgttta
     2881 ccttaaggat gctgcccacg cggataacgt ccgtacaaaa gaggaaatca atcgacaaaa
     2941 acaagaacat agtcaacatc gtgaaggtgg gactccaaga aacgatggtg ctgttgcctt
     3001 ggcacgttcg caaggacgct atactacaga tgatggttat atctttaatg cttctgatat
     3061 catagaggat actggtgatg cttatatcgt tcctcatgga gatcattacc attacattcc
     3121 taagaatgag ttatcagcta gcgagttggc tgctgcagaa gccttcctat ctggtcgggg
     3181 aaatctgtca aattcaagaa cctatcgccg acaaaatagc gataacactt caagaacaaa
     3241 ctgggtacct tctgtaagca atccaggaac tacaaatact aacacaagca acaacagcaa
     3301 cactaacagt caagcaagtc aaagtaatga cattgatagt ctcttgaaac agctctacaa
     3361 actgcctttg agtcaacgac atgtagaatc tgatggcctt gttttcgacc cagcgcaaat
     3421 cacaagtcga acagctagag gtgttgcagt gccacacgga gatcattacc acttcatccc
     3481 ttactctcaa atgtctgaat tggaagaacg aatcgctcgt attattcccc ttcgttatcg
     3541 ttcaaaccat tgggtaccag attcaaggcc agaacaacca agtccacaac cgactccgga
     3601 acctagtcca ggcccgcaac ctgcaccaaa tcttaaaata gactcaaatt cttctttggt
     3661 tagtcagctg gtacgaaaag ttggggaagg atatgtattc gaagaaaagg gcatctctcg
     3721 ttatgtcttt gcgaaagatt taccatctga aactgttaaa aatcttgaaa gtaagttatc
     3781 aaaacaagag agtgtttcac acactttaac tgctaaaaaa gaaaatgttg ctcctcgtga
     3841 ccaagaattt tatgataaag catataatct gttaactgag gctcataaag ccttgtttga
     3901 aaataagggt cgtaattctg atttccaagc cttagacaaa ttattagaac gcttgaatga
     3961 tgaatcgact aataaagaaa aattggtaga tgatttattg gcattcctag caccaattac
     4021 ccatccagag cgacttggca agccaaattc tcaaattgag tatactgaag acgaagttcg
     4081 tattgctcaa ttagctgata agtatacaac gtcagatggt tacatttttg atgaacatga
     4141 tataatcagt gatgaaggag atgcatatgt aacgcctcat atgggccata gtcactggat
     4201 tggaaaagat agcctttctg ataaggaaaa agttgcagct caagcctata ctaaagaaaa
     4261 aggtatccta cctccatctc cagacgcaga tgttaaagca aatccaactg gagatagtgc
     4321 agcagctatt tacaatcgtg tgaaagggga aaaacgaatt ccactcgttc gacttccata
     4381 tatggttgag catacagttg aggttaaaaa cggtaatttg attattcctc ataaggatca
     4441 ttaccataat attaaatttg cttggtttga tgatcacaca tacaaagctc caaatggcta
     4501 taccttggaa gatttgtttg cgacgattaa gtactacgta gaacaccctg acgaacgtcc
     4561 acattctaat gatggatggg gcaatgccag tgagcatgtg ttaggcaaga aagaccacag
     4621 tgaagatcca aataagaact tcaaagcgga tgaagagcca gtagaggaaa cacctgctga
     4681 gccagaagtc cctcaagtag agactgaaaa agtagaagcc caactcaaag aagcagaagt
     4741 tttgcttgca aaagtaacgg attctagtct gaaagccaat gcaacagaaa ctctagctgg
     4801 tttacgaaat aatttgactc ttcaaattat ggataacaat agtatcatgg cagaagcaga
     4861 aaaattactt gcgttgttaa aaggaagtaa tccttcatct gtaagtaagg aaaaaataaa
     4921 ctaatgaaaa at
//
LOCUS       DAVMGZ010000117         4891 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000117 DAVMGZ010000000
VERSION     DAVMGZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.118"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            73..669
                     /locus_tag="VYQ93_001707"
                     /pseudo
     CDS             73..669
                     /locus_tag="VYQ93_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            745..1194
                     /locus_tag="VYQ93_001708"
                     /pseudo
     CDS             745..1194
                     /locus_tag="VYQ93_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            1207..2298
                     /locus_tag="VYQ93_001709"
     CDS             1207..2298
                     /locus_tag="VYQ93_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336360.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQTIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGM"
     gene            2288..3923
                     /locus_tag="VYQ93_001710"
                     /pseudo
     CDS             2288..3923
                     /locus_tag="VYQ93_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4026..4718)
                     /locus_tag="VYQ93_001711"
     CDS             complement(4026..4718)
                     /locus_tag="VYQ93_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV3336361.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1532 a         1042 c          841 g         1476 t
ORIGIN      
        1 tagaattgta ggttttaaaa acgcttacat tgttttaaaa taaacttaaa taacacaacg
       61 gaggtatcca tcatgaaaaa gaaatggatg tattatgctg cttgttcttc taatgaatct
      121 gccgatgaca gttcatctga taaaggagac ggtggttcgc tagtcgttta ttgaccaaac
      181 tcagaaggct taattggagc aactattcct gcctttgaag aaaaatatgg tatcaaaata
      241 gaactgattc aagctggtac tggagaactt ttcaaaaaac tagagtcaga aaaagaagtt
      301 cctgtagctg atgttatctt tggtggttct tatacacaat atgctaccca cggagaactc
      361 tttgaaaact atatttcaaa agaaaatgat aatgttatca aagaatatca aaacacaact
      421 ggcttctcta ctccttatac actagatggt aatgttttaa tcgtccaccc tgatttaact
      481 aaaggcatga acatcgaagg atatagcgac cttctcaaac ctgaactaaa aggaaaaatc
      541 gcaactgctg acccagcaaa ctcttctagc gcctttgctc aattaacaaa tatgctacaa
      601 gctcaaggtg gttacaaaga tgataaggct tggtcttatg taaaagatct tttcacactt
      661 attgatggta tagtaaaatg aaataagaac aggacaaatt aatcaggaca gtcaaatcga
      721 tttctaacaa tgttttagaa gtagatgtgt actattctag tttcaatata ctataaaatc
      781 ggttcaagtt catctggtgt ctataaagca gtcgctgatg gagaaatgac tgttggtctc
      841 tcttatgaag atccagcagt taaactctta aatgacggag ctaacattaa ggtagtctat
      901 ccaaaagaag gaaccgtctt cctaccttct agtgctgcta tcgttaaaaa atctaaaaat
      961 atggaaaatg ccaagaaatt tatcgatttt attatctctc aagaagtaca agatacactt
     1021 ggtacaacca ctactaaccg tcctgttcgt aaaaatgcta aaacaagcga aaacatgaaa
     1081 ccaattgaca aaatcaaaac actcacttaa gattatgatt atgtcatcaa gaataaatca
     1141 gatatcgtta agaaatacaa cgaagtcttt acagatatcc aatctaaaca gtaaaagagg
     1201 ttcactatga gtgagatcga aattattaac gccaaaaaaa tctaccacga tgtccctgtt
     1261 attgagaatt tgaacattac aattccaaaa ggaagtctct ttacccttct tggagcttca
     1321 ggatgtggga aaacgaccct tcttcgtatg attgcaggtt tcaacagtat caaagatgga
     1381 gaattttact tcgatgatac aaaaatcaat aatatggaac ccagcaaacg caatatcggg
     1441 ctggttttcc aaaactacgc tattttccca catttgactg tccgagacaa cgttgctttt
     1501 ggtcttatgc aaaagaaggt tccaaaagaa gaattgattc aacagaccaa caagtatctt
     1561 gaactcatgc aaattgctca atatgcggat cgaaagcccg ataaactcag tggtggacaa
     1621 caacaacgtg tcaccttggc atgcgcctta gcggttaatc caagtgttct cctcatggac
     1681 gagccactta gtaatctgga ggccaaactt cgcttggata tgcgtcaaac catccgagaa
     1741 atccaacacg aagtgggaat tacaactgtt tatgtaaccc acgaccaaga agaagccatg
     1801 gctatttcag accaaattgc tgttatgaaa gatggggtga tccaacaaat cggccgacca
     1861 aaagaactct atcataaacc agctaatgag tttgtggcaa cctttatcgg acgcacaaat
     1921 attatccctg ccaatcttga aaaacggagc gacggcgctt atatcgtctt ttcagatggc
     1981 tatgcccttc gaatgctagc tcttgatcag gttgaggagc aagctattca tgtaagcatt
     2041 cgtcccgaag agtttatcaa agatgaatct ggagatatcg aaggaactat tagcgatagc
     2101 gtctatcttg gactaaatac ggagtatttc attgagacag gttttgcctc aaaaattcaa
     2161 gttagtgaag aatcaacttt tgaagaagat ctacaaaaag gcaatcgtat tcgtctacga
     2221 atcaatacgc aaaaattaaa catcttttct gcagatggtt cccaaaacct gataaaagga
     2281 gtcaaccatg gaatgtaaaa aactaaatat ttggacagcc tcctctttct tcctctttct
     2341 tacctatctt gtctttctcg tttatcctat cgttaccgtg ctcaagcaag cacttataca
     2401 tgaaggacaa ttctcactag ctaattttgt cactttcttt agtaaagcct actactctga
     2461 gacactagtc aacagtttca gggtttccat taccgctact gtcacttcct tagttgtagg
     2521 aaccctatta gcttatctct tctctatgta tgacttcaag gggaagaaat ttctacaaat
     2581 attgattatc attgcttcca tgtcagctcc tttcgtagga gcctactcct ggattctctt
     2641 gctgggacga aatgaggtca tcactaaatt tttgacaaat gccctttatc ttccagctat
     2701 cgatatttat ggattcaaag gaattgtact tgtctttaca ctgcaactat tcccactggt
     2761 atttctatat gttgctggaa caatgaacag tattgacaat tctctacttg aagcagctga
     2821 aagcatgggg tccttcggat ttaaacctat cgtaacggtt gttttacctc tcctagttcc
     2881 aaccttacta gcagctcctt gcttgtattt atgagagcat tctcagactt tggaacgcct
     2941 atgttgattg gcgaagggta tcggactttc cctgtcctga tttataccca atttattagc
     3001 gaggttggag gaaattctgc ttttgcaatt atggcgatta tcattgcctt ggcaattttc
     3061 cttatccaaa aacacattgc aaaccgctac agtttcagca tgaatctgct ccatccaatt
     3121 gagcctaaaa aaactacaaa aggaaaaatg gctgccattt atgcaacagt ctacggaatt
     3181 atctttatct ctgttttacc tcaaatctac ttaatttata cctctttcct aaaaacatca
     3241 ggtatggtat ttgttaaagg ttattctcca aacagttaca agctagcttt caatcgtatg
     3301 ggatctgcta ttttcaatac cattcgtatc cctttgattg ccttagttct agttgttcta
     3361 tttacgacat ttatctccta cctagccgtt agaaaacgga atttgtttac aaacttaatt
     3421 gacagcctca gtatggtacc ttatattgta ccaggaaccg ttctagggat tgccttcatt
     3481 tctctggtct atttggaagt ggatttctta tgattacagg gactgctttc atcttgatta
     3541 tgtctctatc tgccagaaaa ttaccttata ctattcgctc atctgttgct agcttacaac
     3601 aaatagcacc aagtattgaa gaagctgctg aaagcttagg aagtagtcgt ctcaatatct
     3661 ttgctaagat tacaactcca atgatgctat cggatattat ttctggagcc atcctatctt
     3721 gggtcacact gatttcagaa ctctctactt ctatcctcct ctacaatgtc aaaacaagaa
     3781 caatgactgt agctatttat acagaggttc tcagaggaaa ttacggtgta gctgcagcct
     3841 tgtcaactat cctgactgtt ctaacagtag gttccttgct cttgtttatg aaaatctcta
     3901 aaagcaatag cattacactt tagttttcca catcaaaaac agccgaaagg agtaccctcg
     3961 gctgtttttt cttatcttga tattcttatc aaatccctag ctcatggcaa gcaagtctag
     4021 actgattaaa tagaaccttc ttccatcttc tcacgtccta gctctcggta actacggtca
     4081 ccgacaactt caaccctaaa ctgaccgtcc tcatattcaa gaatcgtcac gctaccatta
     4141 tctagaccat gcggatgcat gccattaatc agataaacaa tggttccaat agtcattcca
     4201 tggctgacaa caagggcgtt acctccacct tgatcttcca tttcttttgc aattatttca
     4261 aagccttcct tgattcggcc actgagtttt tcccaacctt cagcccaacc agctgtatcg
     4321 acctctacca agccctcagc cagttcagca taagacaatt ggtgaacgtg gtccacatta
     4381 aagatacgag gaataatgcc catgaaaaga tcgccatcat aggctccatc aaaactaccg
     4441 aaacaccatt ctctgatacg cttgtccatg cgataaggga ttttcccctg caagccaagt
     4501 tcttcaagta taattcccat ggtctgaatg gtacgaccag aatcactcga ataagcgcgc
     4561 tcaaactgta gatcagattc tcgcaaaccg attcctaact cttgaatccc tcgttcacct
     4621 tcagcagtta agggagtatc gctccaacct tgcgcgcgac caatcgtgtt aaacatggtc
     4681 ttgccatgac gtaccaaata caatcgtact tttaccattt tccgtcctcc gtttgcttct
     4741 attatatcat gaatgataaa ataaaagcca cccatacagg cgacttttga aggagattat
     4801 tatgaaaaag tttaggagtt cattaaataa agatattaga tgaaaatcaa attcaaacta
     4861 attcagtgtt atctgtttca aataatatta g
//
LOCUS       DAVMGZ010000118         4767 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000118 DAVMGZ010000000
VERSION     DAVMGZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.119"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            74..778
                     /locus_tag="VYQ93_001712"
     CDS             74..778
                     /locus_tag="VYQ93_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV3336362.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     misc_feature    837..924
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            960..1274
                     /gene="rplU"
                     /locus_tag="VYQ93_001713"
     CDS             960..1274
                     /gene="rplU"
                     /locus_tag="VYQ93_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV3336363.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1290..1634
                     /locus_tag="VYQ93_001714"
     CDS             1290..1634
                     /locus_tag="VYQ93_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV3336364.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            1651..1944
                     /gene="rpmA"
                     /locus_tag="VYQ93_001715"
     CDS             1651..1944
                     /gene="rpmA"
                     /locus_tag="VYQ93_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV3336365.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(2255..2460)
                     /locus_tag="VYQ93_001716"
                     /pseudo
     CDS             complement(2255..2460)
                     /locus_tag="VYQ93_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2897..3814
                     /locus_tag="VYQ93_001717"
     CDS             2897..3814
                     /locus_tag="VYQ93_001717"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV3336366.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            complement(3907..4755)
                     /locus_tag="VYQ93_001718"
     CDS             complement(3907..4755)
                     /locus_tag="VYQ93_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV3336367.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
BASE COUNT         1515 a          818 c          973 g         1461 t
ORIGIN      
        1 aatagtaaaa tctaaatggt agcctacgat tttttagaaa gaaaatggta caatatttct
       61 aagagaaaat acaatgggag gtaaaatgag gttattacct ataagaaaaa tatcacgtca
      121 gtctaaaagg ttagcacttt ttttgacgtt ttgtgctgga tatgtggatg cttacacttt
      181 tattgttcgc gggaataccc ttgtagctgg acaaactgga aatgttgtct ttctttcagt
      241 agaattaatt aaaaataatg tttcggatgt tagggacaag gttctcacct tgctagcgtt
      301 tatgatggga gtctttttat taacgattta taaggaaaaa ttgagaattg tgaaaaaacc
      361 tattctgtcc ttgattccct tggcaatctt atcaatcatt attgcttttg tgccgcaaac
      421 tgtggataat atctatctag tgccgccctt ggccttctgt atgggactgg tgacaactgc
      481 ttttggagaa gtgtcgggta ttgcctataa taacgctttt atgacaggga atatcaaacg
      541 gaccatgctg gcttttggag attatttccg aaccaagcac actccttttt tgcgtgaagg
      601 attcatattt gttagcctgc ttagtagttt tgtccttggc gttgtctttt cagcctattt
      661 gacgattttc tatcatgaaa agaccattct tggtgttcct attatgatga gcgtttttta
      721 cctcagcatg ctttttgcct cttggcagaa aaaagtaaaa gaaaaagctt cattttaggt
      781 gttattactt gtaagaaaat aggagatatg ctatacttga agagttgact atgcacgatc
      841 ctgtgcaacc gcacgaacat cgttgcttga ttattcaagt ttaagtggtt cgtcccacga
      901 tgctaggcga gtcttcacaa aaaatcgggg aaacccataa aaatcatagg aggtgcataa
      961 tgagcacata cgcaattatc aaaactggcg gaaaacaagt taaagttgaa gttggtcaag
     1021 cagtttacgt tgaaaaattg aacgttgaag ctggtcaaga agttactttt aacgaagttg
     1081 ttcttgttgg tggtgaaaac actgttgtcg gaactccact tgttgctgga gctactgtag
     1141 ttggaactgt tgaaaaacaa ggaaaacaaa agaaagtggt tacttacaag tacaaaccta
     1201 aaaaaggtag ccaccgtaaa caaggtcacc gtcaaccata tacaaaagtt gtcatcaacg
     1261 caatcaacgc ttaattttaa ggagaacaca tgatacaggc agtctttgag agagccgaag
     1321 atggcgagct gaggagtgcg gaaattactg gacacgccga gagtggcgaa tacggcttag
     1381 atgtcgtgtg tgcatcggtt tctacgcttg ccattaactt tatcaattct attgagaaat
     1441 ttgcaggcta tgaaccaatc ctagaattaa acgaagatga aggtggctat ctgatggttg
     1501 aaataccaaa agatcttcct tcacaccaga gagaaatgac ccagttattc tttgaatcat
     1561 ttttcttagg tatggcaaac ttatcggaga actattctga gttcgtccaa accagagtta
     1621 tcacagaaaa ctaacacgga ggaaaacatt atgttaaaaa tgactcttaa caacttgcaa
     1681 cttttcgccc acaaaaaagg tggaggttct acatcaaacg gacgtgattc acaagcaaaa
     1741 cgtcttggag ctaaagcagc tgacggacaa actgtaacag gtggatcaat cctttaccgt
     1801 caacgtggta cacacatcta tccaggtgta aacgttggtc gtggtggaga cgatactttg
     1861 ttcgctaaag ttgaaggcgt agtacgcttt gaacgtaaag gacgcgataa aaaacaagtt
     1921 tctgtttacc caatcgcaaa ataaaaaggt tcattgaacc ttttatcccg aaccttgaaa
     1981 tgtaaaggtg aggaagctag aaacagctta aaataaggct ttccgagttt tcggagagcc
     2041 tgttttttta ttttgatacc catacttttt aatatgtata gtggattgat actcttcgaa
     2101 aatcaaattc aaaccacgtc aatttcacct tgccgtactc aagtacagcc tgcggctaac
     2161 ttcctagttt gcactttaat tttcattgag tatgaatctg gaatagaaca ctgttaaaaa
     2221 ttgatatgag tttcctactc gatttcttca gattttattt caattgacta taagaactgc
     2281 tatctggttt caaatattgg cttgtcgtcc cttcacttgt ctgaatggtt tgagccgttc
     2341 cttctgataa gggaaaagat attctttagc tgaaacggtc tgagaatcca agctaacaga
     2401 tggagagaaa aatagcccag tctgaagcac actagatggg agttgttgga aagcagtcat
     2461 ctagtttttc tttgctgttt tggctttttt ctttgtcatc agggaaagtg acttgcttca
     2521 aatcagggag tttaggaaat tcttcaaaca tcttgcccaa gttgtctcat tttgtaaatt
     2581 ttacagccat aggtgtacct cgattctaaa ttctaccttt ttggcaccaa ttagtaattt
     2641 aaagttctca ggagttagtt gcattgcttt ttcatacata acatagtatg acactttatg
     2701 gttaccgaac aagtctaatc actccttgca aaattttgaa gatgtcctac aagtttttga
     2761 gaagaatatt gcaaagttca tagttaagca acgatagaat aaagtgcttt ttgaaaacag
     2821 gtacacttca tgtgaaaaaa acaacatttc agattttctg ttccagaaat agctatcgct
     2881 atgatataat tattttatga ttattactat tcctataaaa aaccaaaaag atattggcac
     2941 accatctgat tcagtcgttg ttctcggcta ttttgatggc atacataagg ggcatcaaga
     3001 attatttcgt gttgccaaca aggctgcgag aaaggattta ttgcctatcg tcgttatgac
     3061 ctttaatgaa tctccaaaga tcgctttaga gccttatcat ccagatttgt ttttgcatat
     3121 tttgaaccct gctgaacgtg aaagaaaatt aaagcgcgaa ggtgtagaag aattatatct
     3181 ccttgatttt agtagtcaat tcgctagtct cactgcacaa gaattctttg caacttatat
     3241 caaggctatg aatgccaaaa ttattgttgc aggttttgat tatacatttg gttctgacaa
     3301 aaaaacagca gaagatttaa aggattactt tgatggagaa gttatcattg ttccacctgt
     3361 agaagatgag aaaggaaaga ttagttcaac tcgtatccgt caagctattt tagatggaaa
     3421 tgtgaaagaa gcaggaaaac ttttgggggc accgcttcca tcaagaggta tggtggttca
     3481 tggtaatgct cgtggtcgta caattggtta tccgacagcg aatttagtgc ttttagaccg
     3541 tacttatatg ccagcagatg gcgtttatgt cgttgatgtt gagattcaaa gacagaagta
     3601 tcgtgctatg gctagtgtcg ggaaaaatgt gacctttgat ggagaagaag cacgttttga
     3661 agtcaatatt tttgatttta atcaagatat ttatggggaa accgtcatgg tttattggct
     3721 tgatcgcatt cgtgatatga ccaaatttga ctcagttgac caattagtgg atcagttaaa
     3781 ggctgatgaa gaagtaactc ggaattggtc ttaagagctt gagtaaataa aacaaaaaag
     3841 aggttgtctg taacccaaaa gatagatgat ttagtctaac ttttgaggtc acgacattac
     3901 ctctttttat tctttttcaa aggtgaagcc ttctcctagg atttcatggg cttctgtaat
     3961 agttataaag gcttgaggat cgattcgatg aatcatttcc ttcgttttca caatttcatt
     4021 tcttccgaca atacagtaga tgattttcaa attttcttta ctatagtagc cttgaccaga
     4081 aataaaagta acacctcttc cgaggtcatc attaatcgcc ttagcaagtt ggtcaggacg
     4141 ttttgtgata atcataaagc ctttgccggc atatcctcct tcaccaatca aatcaataac
     4201 acgagaaaca ataaaatcaa acaaaagcgt gtaggaaacc aatctcaaat ccttgaagat
     4261 taggagaatc aacatgagaa tacaaaaatc taagataaag agcagttttc ctatggatat
     4321 atgagtgtat ttgttgagaa tacgagctag aatatcagtt ccgccagttg tacctccagc
     4381 attaaaaata attccaaggc caattcccaa taggattccc gctataaggg ctgtgattag
     4441 taaatcacct tgaagatcaa tatgaagggg aatatgctca aaaaaagcta accaggcgga
     4501 caaagctaag gttcctagta aactagaata gagggatttg gctccaaaga tcttccaagc
     4561 taggatgaaa aggggaatat taatcagcag gttcatgagg gaaacaggga ttttaaaaag
     4621 ataaaaggtg atgagggtaa tgcctgtcgc ccctccttca aagagatgat ggggaactac
     4681 aaaataagtc agtccaaaag cataaatagc agcacctagt aaaatggtaa aaatggggta
     4741 aattttttta atcataggac acctctt
//
LOCUS       DAVMGZ010000119         4665 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000119 DAVMGZ010000000
VERSION     DAVMGZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.120"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            81..878
                     /locus_tag="VYQ93_001719"
     CDS             81..878
                     /locus_tag="VYQ93_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV3336368.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1026..2285
                     /locus_tag="VYQ93_001720"
                     /pseudo
     CDS             1026..2285
                     /locus_tag="VYQ93_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2381..3655
                     /locus_tag="VYQ93_001721"
     CDS             2381..3655
                     /locus_tag="VYQ93_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV3336369.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3665..4543
                     /gene="truB"
                     /locus_tag="VYQ93_001722"
     CDS             3665..4543
                     /gene="truB"
                     /locus_tag="VYQ93_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV3336370.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
BASE COUNT         1392 a          852 c         1066 g         1355 t
ORIGIN      
        1 ttttatgaaa tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa
       61 aaaagattta ggagtttatt atgatttctt cagaatttat ctcaaagatt gaatttgctt
      121 gcaataagaa agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt
      181 tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta
      241 ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg
      301 cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg
      361 gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct
      421 tgttcaacct tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg
      481 cgaatctgac acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa
      541 atgaattggt cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt
      601 catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca
      661 tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga
      721 aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact
      781 ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg
      841 ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg
      901 tgcttttttc attttcaaaa taaggtaata gctatttctt atatcaaaat atagaaaact
      961 gatatttgta aactataact caaggtgcta caatatcctt aataaaatga tatggaggtc
     1021 accttatgac ttgtgatttt aaatctgaaa ctctacaact acatgctggt caagttgtgg
     1081 ctccagctac taagtctcgt gcagtgccga tttatcaaac aacatttttt gtttttgatg
     1141 acacgtagga aggtgccgat ctgtttgcct tgaggaaacc agggaacatt tatactcgta
     1201 tcaccaatcc tacaacagct gcccttgaag gtggtgttga agcgctagca acagcatcag
     1261 gtatgactgc agtgacttat acgattttgg cgcttgccca tgctggtgac catgtagtgg
     1321 ctgcttcgac tatttacggt ggaaccttca atcttttgaa agaacccctt cctcgttatg
     1381 gtatcacaac aacctttgtc gatgttgata atttggagga agtagaagca gctatcaaag
     1441 acaataccaa gcttgtcttg attgaaacct tgggtaaccc cttgattaat attccagacc
     1501 tggaaaaact ggcagagatt gctcataaac atcaaatccc acttgtgtca gacaatactt
     1561 ttgcaatacc ttatttgatt aacgtcttct ctcatggcgt tgacattgcc attcactctg
     1621 cgactaagtt tatcggtggg catggtacaa ctattggagg aataattgtc gatagtggtc
     1681 gttttgactg gacggcttca gggaaattcc ctcaatttgt tgacgagggt tcaagctgcc
     1741 acaatttgag ctatattcgt gatgtgggtg cagcaacctt tattatagct gttcgagttc
     1801 aattgcttcg tgatacaggt gcagtcttgt caccattcaa tgctttcctc ttgctacaaa
     1861 gacttgaaac tctttcactt cgtgtggaac gccatgtaca aaatgctgag acaattgttg
     1921 attttcttgt caaccatcct aaggtagaaa aggtaaatta tccaaaactt gcagatagtc
     1981 cttatcatgc cttggctgag aaatacttgc caaaaggtgt cggttcaatc tttaccttcc
     2041 acgtcaaagg tgacgaggaa gaagcacgca aggtcattga taatttagaa atcttttctg
     2101 accttgcaaa cgcggcagat gctaaatcgc ttgttgtcca tccagcaaca accactcacg
     2161 gtcaattgtc agaaaaagac ctagaagcag caggtgtcac accaaaccaa attcgtctgt
     2221 caatcggtct tgaaaatgta gaagatttga ttgaagactt acgcttggcc ttggaaaaaa
     2281 tttaaagtaa aagaagataa acagtgggtt ttgactcact gtttttgatt ttccctcagg
     2341 catgatataa tggttacaga agtctagaaa gaggaatgta atgaacgaaa tcaaatgtcc
     2401 caactgtggg gaagtcttta cagtaaatga aagccagtat gctgaacttt tgtcccaagt
     2461 gagaacggca gagtttgata aggaactaca cgataggatg aggcaggaac tggccttggc
     2521 tgagcaaaag gctatgaatg agcaacagac taaactggct cagaaggatc aagaaattgc
     2581 gcaattgcag agtcaaatcc aaaactttga tacagaaaaa gaattggcca agaaagaagt
     2641 tgaacagaca agccatcagg ccttattggc taaggacaag gaagtacagg ctttggagaa
     2701 ccaattggcc actttgcgtt tggagcatga aaatcaactg caaaagaccc tttctgacct
     2761 agaaaaagaa cgcaatcagg tcaaaaatca gctcctactg caagaaaagg aaaatgagtt
     2821 gtctttggct tctgttaagc aaaactacga agcccagctc aaagcagcca gtgaacaagt
     2881 cgagttttat aagaatttta aggctcaaca atctacaaaa gcgattgggg aaagcctaga
     2941 acagtatgca gagagtgagt ttaacaaggt ccgtagtttc gcctttccaa atgcttactt
     3001 tgagaaggat aacaaggtct cttcgcgtgg gtctaaaggg gactttatct tccgtgagtg
     3061 tgatgaaaat ggagttgaaa tcatttctat catgtttgag atgaaaaacg aagcggacgg
     3121 aacagagaag aagcacaaga atgcagattt ttacaaggaa ttggacaagg accgtcggga
     3181 gaagaactgt gagtatgccg ttttggtgac catgcttgag gccgataatg actactttaa
     3241 cacagggatt gttgacgtca gccacgagta tgaaaaaatg tatgttgttc gtcctcaatt
     3301 ctttatccag ttgattggtc tcttacgtaa tgcggcgcta aattccctaa aatacaagca
     3361 ggagttggcc ttggttcgcg agcaaaatat tgacattacg cattttgagg aagatttgga
     3421 tgcctttaag ctagcttttg ctaagaacta taattcagct tcgactaact ttggaaaagc
     3481 tattgatgaa atcgacaagg ccatcaaacg catggaagag gttaagaaat tcctgactac
     3541 atctgaaaac caactccgtc tagctaacaa caaattggaa gatgtctctg ttaaaaaatt
     3601 gacccggaaa aatccaacaa tgaaagcgaa gttcgaagca ctgaaggggg agtagaaagc
     3661 aaaaatgaac ggtattatta acttaaaaaa ggaagcagga atgacctcgc atgatgcggt
     3721 ttttaaactg cgtaagattt tgggaaccaa gaaaattggt catggtggaa ccttggatcc
     3781 ggatgtggtg ggtgttttgc cgattgcggt tggcaaggcg acacgcatgg tcgagtttat
     3841 gcaggacgag ggtaagatct atgaggggga aatcactctg ggctattcca cgacgactga
     3901 ggatgctagt ggggaagtgg tcgcagaaac ccctgttttg tctctcttgg atgaaaagct
     3961 tgttgatgaa gcgattgcta gcttgactgg gcctattact cagattcccc ctatgtattc
     4021 ggcagttaag gttaatggtc gcaagctcta tgagtatgcg cgtgctggtc aggaagtgga
     4081 gcgtccagaa cgtcaggtga ccatttatca atttgagcga acaagtccga tttcttatga
     4141 tggccaactt gcccgattca cttttcgtgt aaaatgcagt aaagggacgt acatccgtac
     4201 tttgtcagtt gatttgggtg aaaagcttgg ttatgcggct catatgtccc atttgactcg
     4261 tactagtgct gctggcttac aattagaaga cgctcttgcc ttggaggaaa ttgctgaaaa
     4321 agtagaggct gggcaattag attttctcca tcctttagag attgggacag gtgaccttgt
     4381 caaagttttc ctaactccag aagaggctac agaagttcgc tttggtcgtt ttattgagct
     4441 agaccaaacg gacaaagaat tggctgcctt tgaagatgat aaattgttag ccattctaga
     4501 aaaacggggc aatctctata agccaaggaa ggtttttagc tagatcgttt aggaataaaa
     4561 atcgggtgat agataataat tgcttgataa aaccccatac taatagtaga atggttttgg
     4621 gaattataat attccaattg ttgcgagttg taggtactca aataa
//
LOCUS       DAVMGZ010000120         4574 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000120 DAVMGZ010000000
VERSION     DAVMGZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.121"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..432
                     /locus_tag="VYQ93_001723"
                     /pseudo
     CDS             1..432
                     /locus_tag="VYQ93_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            599..799
                     /locus_tag="VYQ93_001724"
     CDS             599..799
                     /locus_tag="VYQ93_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336371.1"
                     /translation="MVVSASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            824..1241
                     /locus_tag="VYQ93_001725"
                     /pseudo
     CDS             824..1241
                     /locus_tag="VYQ93_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1472..2179
                     /gene="ccdA2"
                     /locus_tag="VYQ93_001726"
     CDS             1472..2179
                     /gene="ccdA2"
                     /locus_tag="VYQ93_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV3336372.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLSVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2190..2747
                     /gene="sdbB"
                     /locus_tag="VYQ93_001727"
     CDS             2190..2747
                     /gene="sdbB"
                     /locus_tag="VYQ93_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV3336373.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPNFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKIVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3121..4512
                     /locus_tag="VYQ93_001728"
     CDS             3121..4512
                     /locus_tag="VYQ93_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV3336374.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
BASE COUNT         1358 a          833 c          950 g         1433 t
ORIGIN      
        1 ataatccaga cgcttatttg actgaaatag cttctgaatt tggctgtcat ccaactacca
       61 tccactatgc gctcaaagct atgggctaca ctcgaaaaaa agaaccacac ctactatgaa
      121 caaaacccag aaaaagtagc cttatttctt aagaatttta atagtttaaa gcacctagca
      181 cctgtttaga ttgatgaaac aggatttgat acttattttt atcgagaata tggtcgctca
      241 ttaaaaggtc agttaagaag aggcaaagta tctggaagaa gatatcagag gatttctttg
      301 gttgcaggtc taacaaatgg tgaattaatc gctccaatga cttacgaaga gacgatgacg
      361 agcgactttt ttgaagtatg gtttcagaaa tttctcttac caacattaac cacaccatcg
      421 gttattatag taaaatggaa taagaacagg acaaatcgat caggacaatc aaatcaattt
      481 ctagcaatgt tttagaagtc catatgtact attctagttt caatatacca taactgctcc
      541 tagtcaacct gcaagtcctg tcccagctcc aagctcagct ccgactcaag atcctagagt
      601 ggtagtaagc gcttcggcta gctcaacgag cacacaagct caagagcaag ttgacaagtc
      661 tgaacttcgt gccttgagtc aagagttaga ccaacgcttg aaagctttgg caacagtatc
      721 tgatccgaaa atcgatgcaa ccaaggctgt cctcctagat gctcaaaaag ctccggaaga
      781 tagtgccttg acagagtaag gtctgcatac tgcagtagag tctgtgaaga ctgccttgga
      841 ctctctcaaa gatgtgatag caaatgctag cgacagcaag cctacacagg acaagaagga
      901 tgcaaaacaa ggaacggaag atagtaagga ttcagataag atgactgaaa caaactcagt
      961 tccagcagga gtgattgtgg tcagtctact tgccctccta ggcgtgattg ccttctggct
     1021 aattcgccgt aagaaagagt cagaaatcca gcaattaagc acggaattga ccaaggttct
     1081 aggacagcta gatgcagaaa aaagcggata aaaaagtcct tgccaaagcc caaaaccttc
     1141 tccaagaaac ccttgatttc gtgaaagaag aaaatggctc agcagagaca gaaactaaac
     1201 tagtagagga gcttaaagca atccttgaca aactcaagta aaacatgatt tatgaaatgt
     1261 cagacctaaa agacattttc gttcacaaaa gcagcgacga gtaatccttg tcgttgcttt
     1321 ttggctattt ttggggataa gatccgcctt gactagcaaa agaaaagatt ggtccaatag
     1381 tgcccgatac gattttgcgt agcagaaagc ttacaaataa aattgaatat agtaaaatag
     1441 gattagaatt attaagaaag ttggttcttt attggaaacg atagtatttt taatctctgt
     1501 ttttctagca ggtgttttat cctttttttc tccttgtatt tttcctcttc tgtcagtcta
     1561 tgctgggatt ttattggatg atcaagaaag tgcaaaaagc ttttctttgt ttgggagaaa
     1621 ggttctctgg tcaggcttga ttcgaacact ttgctttatc gctggtatct ctctcatttt
     1681 ctttattcta ggctttggtg ctggttactt tggtcatatt ctctatgcaa attggtttcg
     1741 atatggcatg ggagctatta ttatcatttt gggtcttcac cagatggaaa tttttcattt
     1801 gaagaaatta gaagttcaaa aaagttttac ctttaaaaaa tcagattcta atcgttattg
     1861 gtcagctttt ttacttggta ttacctttag ctttggttgg acaccttgta ttggtccagt
     1921 tttaagttct gttttagcac ttgcggcttc tggaggcaat ggcgcttggc aaggagcgat
     1981 ttatactctc atttacactc tgggcatggc ccttcctttc ttggtattgg cactagcttc
     2041 aggtctagtc atgccatatt ttagtaaaat caagcgtcat atgatgctac taaagaaaat
     2101 tggtggtttc ctcattgttt taatgggaat tttgttacta ttaggacaag taaatgttct
     2161 agctggaatt tttgaataaa ggaggacaaa tgaaaaaagt aatgtttgct ggcttaagtc
     2221 tcttgtcatt agttgtattg atggcctgtg gtgaggaaga aactaaaaag actcaagcag
     2281 cacaacagcc aaaacaacaa acgactgtac aacaaattgc tgttggaaaa gatgctccaa
     2341 acttcacatt gcaatccatg gatggcaaag aagttaagtt atctgatttt aagggtaaaa
     2401 aggtttactt gaagttttgg gcttcatggt gtggtccatg caagaaaagt atgccagagt
     2461 tgatggaact agcggcgaaa ccagatcgtg atttcgaaat tcttactgtc attgcaccag
     2521 gaattcaagg tgaaaaaatt gttgagcaat tcccacaatg gttccaggaa caaggatata
     2581 aggatatccc agttctttat gataccaaag caaccacctt ccaagcttat caaattcgaa
     2641 gcattcctac agaatattta attgatagcc aaggaaagat tggaaagatt caatttggtg
     2701 ctatcagtaa tgcggatgca gaagcagcat ttaaagaaat gaactagaat cgataaaatc
     2761 tgattacaag atgtagtcag attttggtaa cttctaaaac taacttccag tttcccacaa
     2821 cctagctttt agtatgagaa aaacgcgtga catcagtgga aatccgtctt agactaactt
     2881 ccactggttt tctttttctt gatatataag ggttcaaaag cgaaaagact cagaaaaagt
     2941 gcacgacaaa tagccctaaa acagagtcgt cttagagtaa cttccagttt tgggagagag
     3001 atggaagtta ctttgagaag ttacggtagt cagatttttt tagaaaaaca ttttataaat
     3061 attcacatat ctcctatatt tatggtaaaa tagaatcatc agtttatttt ggagtcaaag
     3121 atgaatatat ttagaacaaa gaatgttagt ttagataaaa cagagatgca taggcatttg
     3181 aagttatggg atttgatttt gctgggtatc ggagccatgg tagggacagg cgtctttaca
     3241 atcacaggta ctgcagctgc aacacttgct ggcccagccc tagtgatttc aatcgttatt
     3301 tctgccttgt gtgtgggatt atcagccctc ttttttgcag aatttgcctc gcgagtaccc
     3361 gctacaggag gtgcctatag ttacctctat gctatcttag gagaattccc tgcctggttg
     3421 gctggttggt taaccatgat ggagttcatg acagccatat caggcgtagc ttcgggttgg
     3481 gcagcttatt ttaaaggctt gctctctcaa tacgggatag cctttcctca ggctttaaat
     3541 ggtaccttta atcctcaagc agggacattt gttgatttat tgcctattct tgtcttggtc
     3601 ttggtgactt cgcttgtttt actaaatgcc aaggcagcct tacgctttaa ttcgattcta
     3661 gttattttga aattttccgc tttagctctc tttgttttag taggaatttg gaatattaag
     3721 tttgataatt ggagcaattt tgctccttac ggttttggac aaatctatgg agctagcact
     3781 ggtattatgg ctggtgcgtc cttgatgttc ttcggttttt tgggatttga atccatctct
     3841 atggctgtag atgaagtcaa gactcctcaa aaaaatattc ctagagggat tgtcttatcg
     3901 ctttctatcg taaccattct ctatgccttg gtgacccttg ttttgactgg tgtggttcac
     3961 tatagtcatt taaatgtcga tgatgccgtt gcctttgccc ttcgtagtgt tgggattagt
     4021 tgggcagcca actatgtgtc attagtggct atcttgacct tgattacagt ttgtatttcg
     4081 atgacctatg ccctatcgcg tatgatttac agtttagcac gtgacggctt agtgcctgct
     4141 gcctttaagg aactgacgaa gactagcaag ataccaaaga atgctactat cttgacaggt
     4201 ctagcttcag cagtagcagc aggaatgttc ccactagcca gtattgcagc ctttttaaat
     4261 atttgtacct tagcctactt gatcatgttg gcttatggtc tgattcgctt acggaaagaa
     4321 aaaggaatgc ccaaagctgg ggaatttaaa acaccattgg tacctctgtt accaatttta
     4381 tcgatcatca tttgtttatc cttcatgtta cagtataata tgaatacctg gattgctttc
     4441 ctagttgcgt tgttagtagg aagtattatt tacttcactt atggctataa gcattctacc
     4501 atagaagaat aaagtgaaag tctattgagt tgttgacact cagcagattt ttatatgtga
     4561 aagaaatttc aaat
//
LOCUS       DAVMGZ010000121         4528 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000121 DAVMGZ010000000
VERSION     DAVMGZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.122"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            96..329
                     /locus_tag="VYQ93_001729"
     CDS             96..329
                     /locus_tag="VYQ93_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV3336375.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            329..1663
                     /locus_tag="VYQ93_001730"
     CDS             329..1663
                     /locus_tag="VYQ93_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV3336376.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTIAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            1673..1927
                     /locus_tag="VYQ93_001731"
     CDS             1673..1927
                     /locus_tag="VYQ93_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV3336377.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            2023..2418
                     /locus_tag="VYQ93_001732"
     CDS             2023..2418
                     /locus_tag="VYQ93_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV3336378.1"
                     /translation="MSKKLNRKKQLRNGLRRSGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            2830..3279
                     /locus_tag="VYQ93_001733"
     CDS             2830..3279
                     /locus_tag="VYQ93_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEV3336379.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQIPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            3266..3823
                     /locus_tag="VYQ93_001734"
     CDS             3266..3823
                     /locus_tag="VYQ93_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3336380.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKVTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLKSEWEE"
     gene            3825..>4528
                     /locus_tag="VYQ93_001735"
     CDS             3825..>4528
                     /locus_tag="VYQ93_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV3336381.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDINFTYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNA"
BASE COUNT         1393 a          810 c         1043 g         1282 t
ORIGIN      
        1 acaacctcaa aacactgttt tgagcaacct gcggctagct ttctagtttg ctctttgatt
       61 ttcattgagt attagaacat ataatggagg tcgtcatgga caatatcatc gatgtgtcaa
      121 ttcctgttgc agaagtggtg gacaagcatc cagaagtctt ggaaattcta gtggagctgg
      181 gttttaaacc ccttgccaat cccttgatgc gcaatacagt tggtcgtaaa gtatcactta
      241 aacaaggttc taagctagca ggaactccta tggacaagat tgtacgcaca ctggaagcga
      301 atggctacga agtgattgga ttagactaat ggcagatgaa cggattcatg tcctacggga
      361 tattttatta gaattgcata atggcgcctc tcctgagtcg gttcaggagc gttttgatgc
      421 gacctttgcg ggtgtctcag ccattgagat ttcccttatg gagcacgagc tgatgaactc
      481 agactcaggt gtcacctttg aagatgttat ggaactctgt gatgtccatg ccaatctttt
      541 taaaaatgct atcaaaggtg tcgaagtttc agatactgag catccaggtc acccagttcg
      601 tgtcttcaaa gaagaaaatc tggctctccg tgcggccttg attcgcattc gtagattgtt
      661 agatacctat gagtctatgg aagacgagga aatgctggcg gagatgcgta agggtttggt
      721 gcgtcagatg ggacttgtgg gtcaatttga catccattac caacgtaagg aagaactctt
      781 ctttcctatc atggagcgct atggacacga ttcacctccc aaagttatgt ggggagtgga
      841 tgatcagatt agggaactct ttcaaacagc tctaacgata gccaagtcac taccagaagt
      901 gtcaattagc agtgtaaagg aagcttttga agcttttgcg acagagtttg aaagtatgat
      961 tttcaaggaa gagtccatcc tcctcatgat tctccttgag tcttttactc aggatgactg
     1021 gcttcagatt gcggaggaga gcgatgccta tggctatgcc atcatccgtc cgtcagagaa
     1081 atgggtgcca gaacgacaga gctttattga ggaaaagatt gcagaggagc ctgtacagct
     1141 agatacggca gaaggtcaag ttcaacaagt catagatacg ccagaaggcc attttaccat
     1201 tacctttacc cctaaggaaa aggaagctgt gctggaccgc catagtcaac aggcttttgg
     1261 taatggctat ctttcagtcg agcaggccaa tctcatcctc aatcacctcc ctatggagat
     1321 tacctttgtc aataaagatg atattttcca gtattacaat gacaatacgc cagctgatga
     1381 gatgattttc aaacggacgc cgtctcaagt cgggcgcaat gtcgaactct gccatccgcc
     1441 taagtacttg gacaaggtca aaactatcat gaaggggctt cgtgagggga ccaaggacaa
     1501 gtatgaaatg tggttcaagt ctgagtcgcg aggtaagttt gtccacatca cctatgctgc
     1561 agtacacgat gaagacggag aattccaagg agtgttggag tatgttcagg atatccagcc
     1621 ctaccgtgag attgatacgg actattttcg tggattagaa taaggagaaa aaatgagtta
     1681 cgaacaagaa tttatgaagg aatttgaagc ttgggtcaat acccaaatca tgattaacga
     1741 catggcgcac aaggaaagtc aaaaagttta cgaagaagac caggacgagc gtgccaaaga
     1801 tgccatgatt cgctacgaga gtcgcttgga tgcttatcag ttcttgcttg gtaagtttga
     1861 aaacttcaaa gcaggcaagg gattccatga tttgccagaa ggcttgtttg gtgagcgaaa
     1921 ttattaaacg agaaagattc ttgatttttc actaaaatct tgatagaatg tttatgttaa
     1981 atccttgtca gagcagggat tttttattga aaggatttta tcatgtcaaa gaaactcaat
     2041 cgtaaaaaac aattacgaaa tggcctccgt cgctcaggtg ccttttcaag tactgtgact
     2101 aaggttgtag atgagacaaa aaaagtcgtg aagcgtgcag aacagtcagc aagcgcagct
     2161 ggtaaggctg tttctaaaaa agttgaacaa gcagtagaag ctaccaaaga gcaagctcaa
     2221 aaagtagcta attctgtaga agattttgca gcaaacttgg gtggacttcc acttgatcgt
     2281 gccaagactt tctatgatga aggaatcaag tctgcttcag atttcaaaaa ctggactgaa
     2341 aaagaactcc ttgccttgaa aggaatcggc ccagctacca tcaagaaatt gaaagaaaat
     2401 ggcatcaagt tcaagtaatt tttcttgagc cttgcatttc cgagaaaaac ttgctacaat
     2461 agagccatta gaggtgtttt gaatcccaca ttttacagaa agtggcggcg ctgagaagtc
     2521 cacaaatgtg tcaaaactgg ttgctaatga ctgaaaaaat gaaatatttc tgtcttttta
     2581 ttttaaagac taaagatgcg ggcttggccc gaaattgggt ggtaccgcgg ataaacacat
     2641 tcgtccctgt catgtagatg gcagggcttt tcttttgtgt cttagtcaaa ggaggttgtt
     2701 ataaaacaat catttaaaac taataaacta tattatggtt ttcctgtttt tattttagga
     2761 tatcaagatt agaattttgg ctataacatc acgacctgta gttcctctta tagtctagga
     2821 gattgggttg tgattggagt cgttgcgaga gagaatgccg cagagcagat caaacagtat
     2881 caaaaattta ctgtgaatat ttctgatgaa acttctatgc ttgcgatgga gcaggctggt
     2941 tttatcagtc atcaggagaa attggaacgt ttgggagtgc attatgaaat ttctgaacga
     3001 actcagattc ctattttaga cgcctgtcca cttgttttag attgtcgggt agataggatt
     3061 gttgaggaag acggtatttg ccacatcttt gccaagattc ttgagcgact tgttgcccca
     3121 gaactcctgg atgaaaaggg acattttaaa aatcaactgt ttgccccaac ctatttcatg
     3181 ggagatggat atcagcgcgt ttatcgctat ctggataagc gtgtagatat gaagggcagt
     3241 ttcatcaaaa aagcgaggaa gaaggatggc aagaactgag ctgccagata aaatcgaaac
     3301 agaacgtctc gttttacgag tccgtactgt ggcggatgct gaggatatct ttgactatgc
     3361 tagtttgcca gaggtcgcct atccagcagg ttttcctcca gtcaagacct tggaagatga
     3421 gatttattat ctggagcaca ttcttccgga gcgtaatcaa aaggaaaatc tcccagcagg
     3481 ctacgggatt gtcgtcaaag taacggataa gatcgttggc tctgtcgatt tcaaccatcg
     3541 ccatgaagat gatgtgctgg aaatcggcta taccttacac ccagactatt gggggcgagg
     3601 ttatgtgcca gaagctgcgc gtgccttgat tgacttagcc tttaaagatt tgggtcttca
     3661 caagattgaa ctaacttgct ttggatataa ccttcaaagt aaacgagtcg cggaaaagct
     3721 tggctttacc ctcgaagctc gcataagaga ccgaaaggat gttcaaggaa accgctgtga
     3781 cagtctgata tatggcttgc tgaagagcga gtgggaggaa taagatgagc gatgtaaaag
     3841 aggaagtaag ctctctatct gagaagcagt tacgccaaat tgatgttgag tatgctgaat
     3901 taaatgatag tgatattatc gagcgattag cttatttaga aattaacaac aatgaaaaac
     3961 gtattgtcat ctctgatatt gaaccaacca aggaaataat gtcagtctcg gaccaaattt
     4021 ttgagattca aaagaatttt caaaaaatca agaatatgtt cgaattgttt atttcagatg
     4081 tctcggattt tttaagtatt aaaaataaac ttgagtcaaa ggaattagaa attgaagagg
     4141 cagatgtaaa tcgtttcatg attcatttat tatccagtgg aaaattgttt gtcgatttta
     4201 acgaaaacca aattaaacaa aaatattcca aagatagtga ggagtttgat tgtatacatg
     4261 gttttgcaag ctatcagtat gatatcaatt ttacttatcg attttgccat tctttgagaa
     4321 attattcgca acatactgac ttaccaataa atgaggtaaa agctgttagc ccagatgatg
     4381 agactgttat aatagatttc tatattgatt tagattatct tttaaattcc aactttaaat
     4441 ggaaaaaatt gaaaggggag ttgataaaac taaaccaaga aacatctaag attgacgcta
     4501 tagctctagt gaaagaatac tttaatgc
//
LOCUS       DAVMGZ010000122         4461 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000122 DAVMGZ010000000
VERSION     DAVMGZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.123"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            21..188
                     /locus_tag="VYQ93_001736"
     CDS             21..188
                     /locus_tag="VYQ93_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEV3336382.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(248..601)
                     /locus_tag="VYQ93_001737"
     CDS             complement(248..601)
                     /locus_tag="VYQ93_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3336383.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1106..1528
                     /locus_tag="VYQ93_001738"
     CDS             1106..1528
                     /locus_tag="VYQ93_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV3336384.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            1525..1755
                     /locus_tag="VYQ93_001739"
     CDS             1525..1755
                     /locus_tag="VYQ93_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3336385.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            2216..2419
                     /locus_tag="VYQ93_001740"
     CDS             2216..2419
                     /locus_tag="VYQ93_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEV3336386.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            2501..3718
                     /locus_tag="VYQ93_001741"
     CDS             2501..3718
                     /locus_tag="VYQ93_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3336387.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(3880..>4461)
                     /locus_tag="VYQ93_001742"
     CDS             complement(3880..>4461)
                     /locus_tag="VYQ93_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3336388.1"
                     /translation="YIFQQVNVLLVSLYLLKLLCISELTILQILYCASLISFLWMYGQ
                     RKQVAKVNMETRIKWIGIGFVSLLIISLYFSLIHAQESTNQANLIGLQHQVPWFSFLL
                     FLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTILAWCLYVSLGMFLG
                     MVRYKSDLWGSMGLHLVWNLLVYSLLLFNKNKV"
BASE COUNT         1572 a          774 c          842 g         1273 t
ORIGIN      
        1 ttataaacta caagtggata ttgtgtcctg tatgtggaaa taaaacacga ttaaagataa
       61 gggaagatac tgaattaaaa aaattccccc tctattgtcc gaaatgcaga caagaaaatt
      121 taattgaaat aaagcagttc aaagtaactg tgattacaga gccagacgca aagacgcaga
      181 gccgataaaa tgagattaat acaatctcat tttatcggct ctttccgtta tgtatggatt
      241 cttttaatta gtcttcgatg tttcttgctt cgttgatacc gctggctaaa gattccatta
      301 aggatagttc tttgtctgta aagctatcca tgtatttctc tatctgtaat cgtcgggtgc
      361 tttttaccaa gttattagca ggtaagaaaa attcatcaac ggaaacatga agtaacgata
      421 caaggtcata aagaacttgt atgctggggt gttgcccttt attttcaata ttagttaagt
      481 accgtgggtc aatttcaatc aatgctccca cttgttcacg agttaaacct cgtttcaatc
      541 gagcttcttt aatggctaaa ccaaaggctc taaaatcata tttatcttct tttttacgca
      601 tagtagacca cctctataca ttttattgtt cctactgaat taaaaacagg tatagaaaaa
      661 cgtgttatat ggtttatagg tttatattta ataaaaagca ctactaaacg ccaataaaaa
      721 aaaccgttat atggtagtgc tatttacgct gttaaaatat tgtatattac ttccaaatgg
      781 cggtttgttg gaggtcaacg tcgccatgaa gtacatcata tacaataaat ttccttacat
      841 tgggttcttg tcaaaaaaag tcgtctatct gcaatagata agtacgtcca ccaatgtggt
      901 tttataaatc atatagatag aataacagaa gcatgtaaac agagaaataa atctgtttat
      961 atgctttttt ggctattcag aactttttta caaagtttat ttatcagtaa tgcaacaaat
     1021 ccccctttca cattgggact aagagtgaaa ggagataaac gagcaaggct cacttccttt
     1081 cctagacaga aagggggtga gaaacatgaa accatcttct tttcagacca caatagaaaa
     1141 tcagtttgac tatatctgta aacgtgctat ggaagacgag cgaaagaatt atatgcttta
     1201 tctttcaagg attgcaaagc gtgaggtgtc cttttcggat gttggcgatt atcttgttag
     1261 ccagtttgcg acaacagata actattcaac tgactttcag atttttacac tcaatgggtt
     1321 atcagtaggc gttgaaaatg atttgttgag tgaagcatta cgtgagttgc cagacaagaa
     1381 acgtgaaatt ctactgctgt tttactttat ggacatgagc gattcagaaa ttgcagacct
     1441 gttgaaattg aaccgttcta ctgtctatcg gcatagaacc agtggactag ccttaattaa
     1501 aaagtttatg gaggaatttg aagaatgaaa acacaatatc ctatgattcc ctttcctctc
     1561 attgtaaagg caacagatgg cgataccgaa gcgattaacc agattctaca tcattacaga
     1621 gggtacataa cgaagcgttc cctacgactt atgaaagatg aatatggcaa tcaaagtatg
     1681 gtcgttgatg aagtcttacg tggaagaatg gaaaccagac tgattacaaa gattttgtca
     1741 tttgaaatta agtaatatcc tctctccttt cgtggaagcg tgctaaacca ttccacgctt
     1801 cccgaacagg gaggtttgtt attccaccaa agcatattga gctttcaatg tgttttgata
     1861 ggctaacgag ccattgttct ttgaaaactg aataaaagta atcgaatacg tttcgataag
     1921 aaaagagcca acggaactaa ccgccatgac ctatcttata aagatagcga gcgattcatg
     1981 ttagtgatcc gagaagcaat ctttagcagg attgcctgca acgacattct tatcgtgata
     2041 atgatactcc catacagtca atagtccgag cgtgataaaa ccgtcgcagg caatgagtat
     2101 ggctacatga gaaccatgca ggggtggaac tcccgtgagc tttgctaaag ctgttcgatt
     2161 gctggtaaaa caacttttat gaaatccaaa taagtgattt ggaaaggagg attttatgaa
     2221 gcagactgac attcctattt gggaacgtta taccctaacc attgaagaag cgtcaaaata
     2281 ttttcgtatt ggcgaaaaca agctacgacg cttggcagag gaaaataaaa atgcaaattg
     2341 gctgattatg aatggcaatc gtattcagat taaacgaaaa caatttgaaa aaattataga
     2401 tacattggac gcaatctagc gtcgccaaag ggtcttgtat atgataaaat agtattaagt
     2461 cgtatcaagg ctctttccat aaaggaaagg agcaaatgcc atgtcagaaa aaagacgtga
     2521 caataaaggt cgaatcttaa agactggaga gagccaacga aaagacggaa gatacttata
     2581 caaatatata gattcatttg gagaaccgca atttgtttac tcgtggaaac ttgtggctac
     2641 agaccgagta ccagcaggaa agcgtgattg tatctcactt agagagaaaa tcgcagagtt
     2701 acagaaagac attcatgatg gtattgatgt tgtaggaaag aaaatgacac tctgccagct
     2761 ttacgcaaaa cagaacgctc aaagaccaaa ggttagaaaa aacactgaaa ctggacgcaa
     2821 atatcttatg gatattttga agaaagacaa gttaggtgta agaagtattg acagtattaa
     2881 gccatcagac gctaaagaat gggctattag aatgagtgaa aatggttatg cttatcaaac
     2941 catcaataac tacaaacgtt ctttaaaggc ttcattctat attgctatac aagatgattg
     3001 tgttcggaag aatccatttg actttcaact gaaagcagtt cttgatgatg atactgtccc
     3061 taagaccgta ctaacagaag aacaggaaga aaaactgtta gcctttgcaa aagctgataa
     3121 aacctacagc aaaaattatg atgaaattct gatactctta aaaacaggtc ttcgtatttc
     3181 agagtttggt ggtttgacac ttccagattt agattttgag aatcgtcttg tcaatataga
     3241 ccatcagcta ttgagagata ctgaaattgg gtactacatt gaaacaccaa agaccaaaag
     3301 tggcgaacgt caagttccta tggttgaaga agcctatcaa gcatttaagc gagtgttagc
     3361 gaatcgaaag aatgataagc gtgttgagat tgatggatat agtgatttcc tctttcttaa
     3421 tagaaagaac tatccaaaag tggcaagtga ttacaacggc atgatgaaag gtcttgttaa
     3481 gaaatacaat aagtataacg aggataaatt gccacacatc actccacata gtttgcgaca
     3541 tacattctgt accaactatg caaatgcagg aatgaatcca aaggcattac agtacattat
     3601 gggacatgct aatatagcca tgacgctgaa ctattacgca catgcaacat tcgattctgc
     3661 aatggcagaa atgaaacgct tgaataaaga gaagcaacag gagcgtcttg ttgcttagta
     3721 gtacaaatga atttactact tatttaccac ttctgacagc taagacatga ggaaatatgc
     3781 aaagaaacgt gaagtatctt cctacagtaa aaatactcga aagcacatag aataaggctt
     3841 tacgagcatt taagaaaata taaaaagata attagaaatt tatactttgt ttttattaaa
     3901 aagcagcaaa ctataaacta gtaggttcca cactaaatgt agccccatac tgccccacaa
     3961 gtccgattta tagcgtacca tccctaaaaa cattcccaat gaaacatata aacaccaagc
     4021 tagaatggtt cctggatgat gagctaaggc aaataaaaca cttgtcaaag caactcgaat
     4081 atctaatttt ctaaccaagt tccataaaat ttctctatac aaaaattctt caaccatact
     4141 cgcattgatt aagaacaata aaaatgaaaa ccaaggaact tgatgttgaa gcccaattaa
     4201 gtttgcttga tttgtgcttt cttgagcatg aatcagacta aaatatagac tgataatcag
     4261 taggctaacg aatccaatac caatccactt tatcctagtt tccatattga ccttggccac
     4321 ttgttttcgt tgaccataca tccataaaaa agaaatgagc gacgcacaat agagaatctg
     4381 tagtatggtt aactcactaa tacaaagcaa tttcagtaag tatagggata ccaataaaac
     4441 atttacttgt tggaatatat a
//
LOCUS       DAVMGZ010000123         4454 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000123 DAVMGZ010000000
VERSION     DAVMGZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.124"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            262..2595
                     /locus_tag="VYQ93_001743"
     CDS             262..2595
                     /locus_tag="VYQ93_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV3336389.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            2608..4071
                     /locus_tag="VYQ93_001744"
     CDS             2608..4071
                     /locus_tag="VYQ93_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV3336390.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCDFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            4071..>4454
                     /locus_tag="VYQ93_001745"
     CDS             4071..>4454
                     /locus_tag="VYQ93_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3336391.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTE"
BASE COUNT         1553 a          666 c          932 g         1303 t
ORIGIN      
        1 taaatgtgat tatttatttt tgaaaaatag ctcaccagcc cgactgaaag tgcttataga
       61 atgataataa gtcgcctgcc gaaaacatcg gaaaatagcg gtgttatgcg gagataatct
      121 gacgcgatgc gaaagtatat tgcatactta ttttcaacaa tttagcagag tatttttata
      181 agtgtgatat aatagaagta taatttgttc tgatagttta ttttatggag aagtagattt
      241 ttagaatgcg gagggttcaa tatggttgag tttataaagt ctaagaaaga aatgagtgag
      301 gaggatatta aagcaaattt catcactcct gctattgtat ccaaaggatg gaaaaatggt
      361 gagcatatcg cttacgaaga atacttcact gatggtcgaa ttgaggttag aggagataag
      421 gctcgtcgta aagaaggaaa aaaatcagac tattcactgt attaccaatt tggaactcga
      481 attgcaattg ttgaggcaaa ggataataaa cacagcgttc gagcaggatt acaacaagct
      541 attgaatatg gagagatttt agatgttcca tttgtttatt cttcgaatgg tgatggcttt
      601 attgaacacg accgtatcac gagagaagaa cgtgagctgg agttagacga attccctact
      661 cgtgaagaat tattttctcg tatgacgaag gaaaaaggat tgacgtacga aattacagaa
      721 gctatctcaa ctccatacta tacagacgcc ttctcaatga aaacgccacg ctattatcag
      781 caaatagcta tcaaccgtac tattgaaaca gttgccagag gacaaaaacg agtaatgttt
      841 gtgatggcaa caggaacagg gaaaacgttc atggcttttc aaattattca tcgccttcga
      901 aaagctggtt tggctaaacg agttttattc ttagcagata gaaacatctt agtagaccaa
      961 acgatggctg aagactttaa gccattcgaa aaggtaatga cgaaaattac accaaaactt
     1021 ttgactgctc ctgaaaaatt aaattctttc gaaatttatc tagggcttta tcagcaacta
     1081 actggtgaag atggaactga aacacattat caaaaatttg acaaagactt ctttgattta
     1141 atcgtaattg atgaagcgca ccgtggttca gctaaggaaa acagtaactg gcgtaaggta
     1201 attgattatt tcagttctgc gacacagatt gggatgaccg ctactcctaa agaaaccaag
     1261 aatgcttcca atacggaata ctttggtgag ccaatctata cttatagttt aaaacaggga
     1321 atcgaggatg gttttttggc tccatatcgt gttatgaggg ttaatttaga tgtggatgtg
     1381 gatggttatc gtccagaaac tggaaaagtt gatgctaacg gacaattaat agaagatagg
     1441 tactacggca ggaaagattt tgataaaacc attgtcattg atgatagaac gcaaagagtt
     1501 gccaagtttg tttctgatta tatgaagcaa aacaatgcac gatttgataa aacaattgtt
     1561 ttttgtgttg atattgacca tgccgagcga atgcgtgctg cacttgtaaa agagaatcta
     1621 gacttagtcc aagaagacta tcgttatgtc atgcaagtaa ctggtgacaa cgctgaagga
     1681 aaagctcaac tggataactt tatggatgtc aattctaatt ttcccgctat tgtaacaacg
     1741 tctaaattat taacgacagg agttaatgct aaaacatgtc gtttgattgt tttagactct
     1801 aatatccaat ccatgactga atttaaacaa attattggtc gtggcacacg tctttatcct
     1861 caaaagggga aagaattttt tacgattatt gattttcgaa atgttaccaa tttgtttgct
     1921 gaccctgatt ttgatggtga gccagtgaag gtgctggaaa caggtgcgaa aacagtcagt
     1981 ggttctacgc ccggtttcgt agatgaggaa ggtgacccag tagaaaaata tatcgttaca
     2041 gacaagcagg ttaccattct taattctact gttcaagtat tggatgaaaa cgggaaactg
     2101 attaccgaaa gcctgaccga ctacactcgg aagaatatct taggtagcta cgccactttg
     2161 aacgatttta tcacagtttg gcatacggcg gataaaaaga agcttatctt agacgaactt
     2221 tataaaaaag gagtttatct agatgctatt cgagagtcgg agggaatatc agaacaagaa
     2281 atcgatgatt ttgatttact cctaaaactt gcctatggtc aaaaagaatt aaccaaaacg
     2341 gaacgtatca ataaactcaa acaaagcgga tatttatata aatatagtga ggaagcgcgt
     2401 gctgttttgg aaattttact gaacaaatac atggataaag gtattggaga actcgaaagc
     2461 attgaaacat taaaacttcc agaatttcag atatatggtg gaaccttcaa aatcatcaat
     2521 acttattttg gagataaaaa acgatattta caagcaatta aagaattgga gcaagagcta
     2581 tttacagtag cttaatgaaa ggaaagtatg tcaattacat catttgtaaa aagaattcaa
     2641 gatatcactc gaaacgatgc tggtgttaat ggtgatgctc aacgtattga gcaaatgtct
     2701 tggttattat tcttaaaaat ttatgatagc cgtgaaatgg tttgggaatt agaagaagac
     2761 gagtatgagt caattatccc agaggaatta aaatggcgaa attgggctca tgctcaaaat
     2821 ggggaacggg tattgacagg cgatgaatta cttgattttg tcaataacaa gttattcaaa
     2881 gagttgaaag agcttgaaat aacttcaatt atgcctattc gaaaaacgat tgttaaatca
     2941 gcttttgaag atgcgaacaa ctatatgaaa aatggcgtct tgttacgcca agtcatcaat
     3001 gttattgatg aagttgattt caatagccct gaagatcgtc attcgtttaa tgatatttac
     3061 gaaaaaattc ttaaagatat tcaaaatgct gggaactcag gagaatttta tacgccacgt
     3121 gcagcgactg attttattgc cgaagttctt aacccaaaac ttggagaatc aatggcagac
     3181 cttgcttgcg gaacaggagg cttcttgact tcgactctga accgtttaag tagtcaacgt
     3241 aaaactagtg aagataccaa aaaatataat acagctgttt ttggtattga aaagaaagca
     3301 tttcctcatc ttttagcagt tacaaatctg tttcttcacg aaattgatga ccctaaaatt
     3361 gttcatggaa atactttgga gaaaaatgtt cgtgaatata cggatgatga aaaatttgac
     3421 attattatga tgaatccacc ttttggaggg tcagaattag aaacaataaa aaataacttt
     3481 ccagcagaat tacggagttc tgaaacagct gatttattta tggctgtcat tatgtatcgt
     3541 ttgaaagaaa atggtcgtgt tggagttatt ttacctgatg gttttctatt tggtgaaggt
     3601 gtaaaaactc gcttgaaaca aaaactggta gatgagttca acttgcatac gattattagg
     3661 ttgcctcata gtgtctttgc accgtataca ggaatccata cgaacattct tttctttgat
     3721 aaaacaaaga aaacagaaga aacttggttt tatcgtttag atatgccaga tggttataaa
     3781 aatttctcga aaactaagcc gatgaagtca gaacacttca atcctgttcg tgactggtgg
     3841 gaaaatcgtg aagagattct ggaaggtaag ttctacaaat ctaaatcatt tacacctagt
     3901 gaattggctg agttgaatta taatttagac cagtgtgact ttccaaaaga ggaagaggaa
     3961 atcttaaatc cctttgagtt gattcagaat tatcaagcgg aaagagcaac tttaaatcat
     4021 aagattgata atgtattagc tgatattttg cagttgttgg aggacaaata atgacaccag
     4081 aacaacttaa agcaagtatt ctccaaagag cgatggaagg gaaattagtg ccgcaaaatc
     4141 ccaatgacga acctgcaagt gaattattaa agagaattaa agctgaaaaa gaaaaactta
     4201 tcagtgaagg aaaaatcaaa cgagataaaa aggaaactga gatatttcgt ggtgatgatg
     4261 ggaaacatta tgggaagttt gctgatggaa gtactcaaga aattgatgtt ccttatgata
     4321 ttcctgatac ttgggagtgg gtgaggtttt ctacattggt tgaaattgtc agaggtggct
     4381 ctccacgacc aatcaaagat tatcttactt ctgaagtaga tggaataaat tggataaaaa
     4441 taggtgatac tgaa
//
LOCUS       DAVMGZ010000124         4453 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000124 DAVMGZ010000000
VERSION     DAVMGZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.125"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..137
                     /locus_tag="VYQ93_001746"
     CDS             <1..137
                     /locus_tag="VYQ93_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3336392.1"
                     /translation="GKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKGERNITNGFK
                     "
     gene            124..330
                     /locus_tag="VYQ93_001747"
     CDS             124..330
                     /locus_tag="VYQ93_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336393.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISRVCYKGTQFSLIAVNSDMSLGR"
     gene            376..735
                     /locus_tag="VYQ93_001748"
     CDS             376..735
                     /locus_tag="VYQ93_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336394.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKETVLG"
     gene            863..1288
                     /locus_tag="VYQ93_001749"
     CDS             863..1288
                     /locus_tag="VYQ93_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336395.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            1696..2280
                     /locus_tag="VYQ93_001750"
     CDS             1696..2280
                     /locus_tag="VYQ93_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336396.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            2284..2481
                     /locus_tag="VYQ93_001751"
     CDS             2284..2481
                     /locus_tag="VYQ93_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336397.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            2471..3022
                     /locus_tag="VYQ93_001752"
     CDS             2471..3022
                     /locus_tag="VYQ93_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336398.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            3081..3341
                     /locus_tag="VYQ93_001753"
     CDS             3081..3341
                     /locus_tag="VYQ93_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336399.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(4082..4363)
                     /locus_tag="VYQ93_001754"
                     /pseudo
     CDS             complement(4082..4363)
                     /locus_tag="VYQ93_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1496 a          641 c          880 g         1436 t
ORIGIN      
        1 ttggaaaaag aataaaagaa cttagaatat cttcaaatct tactcaagat aagattgctg
       61 agtatttgtc tttgaatcaa agcatgattg ccaaaatgga aaaaggtgaa aggaatatca
      121 cgaatggatt taagtaataa agctccaaat cttagaaaaa agttgggagc tgatggtgaa
      181 tcgccgatag atatttttaa attggtacaa aagatagaaa atttgacgct ggtattttat
      241 ggactcggaa agaatattag cagagtctgt tataaaggaa ctcagttcag tctcattgca
      301 gtcaattcag acatgtcatt aggaaggtaa agattttctt tagcacatga actgtatcat
      361 ctttattatg atgaggtgaa gaagagttca gtcagtctta tcttgattgg tgaaggagat
      421 gaaactgaaa gaaaagcgga tcagtttgct tcttattttt taattttccc atcttcactg
      481 tataggatgg ttgaggaaat tagagaaaat gccaatagaa ctcatcttga agtagaagat
      541 attataaaat tgggtcagtt ttatggtatc agtcataaag ctatgttata tagattgagg
      601 aatgatggat accttgatgc agaagaaatt aaaaatatgg atattagtgt tatagagaca
      661 gcttcaagat taggctatga tacaagttta tatcgtcctt tgtcagaaag taaaaaggaa
      721 acggtattag gataatatat taatttaact gaacaacttt tagaaaataa cagaatttcg
      781 caagggaagt atgaggaact gttactagat gctttcagat atgatattgt atatgggcta
      841 gatgaagagg gggagttgtc gtttgactag tcgtgtattt attgatgcag attgtatttc
      901 agcattttta tgggttggca ctgaacatct tttagaaaag ctctattcgg gtaaaattgt
      961 tattccacaa gaggtgtatg atgaaatcaa tatacctaca attccccatt taaaatctag
     1021 gatagaccag ttggtaacta agggttcagc tgagattgtg agcatagaca ttggaactga
     1081 agaatacgca ttatatagag atttaacaag aaattatgat agtaacaaga ttattggtaa
     1141 gggagaagcg acttctattt tcttagcgaa aaagcataat gggatattag gaagtaataa
     1201 cctaagagat gttaaaccat atgtagaaga attttcttta gaacatatga caacaggaga
     1261 tatactgatt gaagcgttta aagcgtaatt tattactgaa caagagggca atcatatctg
     1321 gaataatatg cttaaaaaga gaaggaaaat tggtgcaaat tcattttcag actatcttcg
     1381 tggaagtatt caccaaaata gacaaaaata aatttggata aatcgaactc actattcagg
     1441 aggcatatga gcaattcgaa aaagaaaagt gtcaaattga gcctatagga gtagaagtga
     1501 aatagtaagt cctgcatagt ggatgagaga aaagttctcc ttgaagtttt cctgaactat
     1561 cagtcgcatg tcaaacgata tgtagggtaa tgtgagaggg gatagcgagt agtttttggt
     1621 tattttatca aaaaacttat attttattat accgaatgat aaaatataat aaaaatgata
     1681 gaataaggaa aaaacatgaa tgtcaaaaag ataatgtcaa tttttcaatc cttttatgtt
     1741 gatgtcagta ttgaggaact gactttgact ttaccaatca gttttgtaaa aaggtttgag
     1801 tatactcaaa tgacttttca taaggaatca tttttattga ttaaagaaaa gagaaggggg
     1861 agtttgagtt catttgttac tcaggctcgc actatgggtg aaaaagccaa tatggatgtt
     1921 gttttggtgt tttcgaagtt atcagacagt gaaaaaaagc aattacttca agctagagtt
     1981 ccgtttgtag actttaaggg aaacctcttc ttccctccat tgggactagt actcaatgcg
     2041 aatgatactg aaatccctaa ggaattaaca cctagcgaac aattaacgtg gattgccttt
     2101 ttattgccag atggcgatat aaagcagata aaatctgttt ctaaccttct atatggtggt
     2161 gcttatgctt tgtcgcattc aactttttta gctgaaacgg atgaaaatac tagctatgtc
     2221 atatggcaga gaaaattcaa tcagttatcc ttgccacttt ctcagcatgt tttaaaatga
     2281 aagatgctag agatatggaa atatcgtcct tttgtatctg agttttggaa tgattttaaa
     2341 aataatcatg ataaacaatt tgtagatccg atttctcttt atttgacctt aaaagatgat
     2401 gatgacccac gtatagagga agagagtgaa gcactagaaa atatgatatt acagtatctg
     2461 ggagaagatg atgccagcta atacgaaagt tatttttcaa gaaatgtttg cggattttca
     2521 gaactattat gttctgattg ggggaactgc tacctctatc gtattggatt cgcaaggatt
     2581 taaaagtcgc acaacaaaag attatgatat ggtcatcatt tatgaagtaa aaaataagga
     2641 attttatact accttgaatc attttttaga attgggagag tatcaaggaa gtcagaaaga
     2701 tgagaaagcg cagctttttc gatttacaac aactaatcct gagtttcctt ctatgattga
     2761 actatttagt atcttaccag aatatccatt aaagaaggac ggtcgagaaa ttcccttaca
     2821 ttttgaccaa gatgctagtt tatcagcctt attattggat gaagattatt ataatatatt
     2881 ggtgcatgaa aaagaaacca ttcaggggta ttcggtattg agtaattgtg gtttatactc
     2941 ttcgaaaatc tcttcaaacc acgtcagctt ccatctacaa cctcaaaaca gtgttttgag
     3001 cagcctgcag ctagcttcct agtttgctct ttgattttca ttgagtatta attattttta
     3061 aggctaaagc ttggctggat atgagggaac gctctgctac aggtgctcaa ggtttaagta
     3121 agtccattaa aaagcatttg aatgacatta cccgtttgac agcttccttg ctaggagatg
     3181 aaaagttatc ggctataaca tcaagtagtg cggtaaaagc agacatacac cgctttgtga
     3241 tagaattaga gcctgtgaag tcaactattc ttcaaaatga tgacatttca ttggatcaaa
     3301 atgaaatttt tgaaattctg aaaaattttc tcgatggtta aaataattgt agcgagatgg
     3361 ctatattgaa ttcgtctata tctggaaact agaaaaaact tcaatttcag gagaaaatga
     3421 agtcaatctt cccacaatca aacgtatagt atcaaggttt ttcaagacct gatattatgc
     3481 gttttttgct tttcaaaact ttttgcctag tctttgtttt tatcctctag tcacttgatt
     3541 tgtttcaggt ggttttttag tatagtgaaa tgaaacgaga acaggacaaa ttgatcagga
     3601 cagtcaaatc gatttctaac aatgttttag aagcagagat gtactattct agtttcaatc
     3661 tactatagtt aaatctgcgg tcaagtctac tggtgaatct atgattgtaa tactcttcca
     3721 aaatctcatc aaccacgtca gtctcgcctt gcagtctgta tcttactgac caagctagtg
     3781 atggacttag aataggtgat ttggagcgtc ctattggcta ggaaatgctg atcatagtcc
     3841 tttgctgagg ctagggtgtt tcaacattca acactcaact ggttgatcta gttgatagga
     3901 agggaattac tataaaatac tcaggcttcc atcatatttt ttgaaacgat tgtgtaatca
     3961 aaatgtacca atattgtagt attagtacag aagatgttgt gaatggataa atatatcata
     4021 actgctgtct caaaaagatt tcatatgtct gtgcatatat aatagacttc ctgcgaaacc
     4081 aaaatcctag ttcatgattg ataataccag caatcaaatt cattcgtaat ccgaagcgtt
     4141 tacgatgatt tcgataggtt gttgaaaaca ttttaaacat ttctactttg gcaaagatgt
     4201 tctcaacctt gcttctctcc ttagatagcg catggttata ggctttatct tcagctatta
     4261 gcggcttgag tttgctggat ttacgtggag tttgtgcttg aggatatatc ttcatgagcc
     4321 cttgataacc actgtcagcc aagattttac cagcttgtcc gacttttttg ataagctgtt
     4381 tttaatacag ctaacatctc ttcaaaagtg gtacgctgaa cactaacaag acgcttaaat
     4441 cgtgcatcag tta
//
LOCUS       DAVMGZ010000125         4446 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000125 DAVMGZ010000000
VERSION     DAVMGZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.126"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            113..538
                     /gene="lacA"
                     /locus_tag="VYQ93_001755"
     CDS             113..538
                     /gene="lacA"
                     /locus_tag="VYQ93_001755"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV3336400.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            569..1084
                     /gene="lacB"
                     /locus_tag="VYQ93_001756"
     CDS             569..1084
                     /gene="lacB"
                     /locus_tag="VYQ93_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV3336401.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQIDANFFTEF
                     LEKWDRGEYHD"
     gene            1095..2024
                     /locus_tag="VYQ93_001757"
     CDS             1095..2024
                     /locus_tag="VYQ93_001757"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV3336402.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            2026..3006
                     /gene="lacD"
                     /locus_tag="VYQ93_001758"
     CDS             2026..3006
                     /gene="lacD"
                     /locus_tag="VYQ93_001758"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV3336403.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3240..4076
                     /locus_tag="VYQ93_001759"
     CDS             3240..4076
                     /locus_tag="VYQ93_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV3336404.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYCQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4112..4429
                     /locus_tag="VYQ93_001760"
     CDS             4112..4429
                     /locus_tag="VYQ93_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV3336405.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
BASE COUNT         1453 a          753 c          950 g         1290 t
ORIGIN      
        1 tcttcaaaca atagtagtat attatgtttg tgaggactaa ttgactgttt tttaaagaca
       61 gccaatacac tgctggaatc cagcatagaa aaaataaaaa ggagtataca atatgtctat
      121 tgttatcggt gcagatgctg caggtttgag attgaaagaa gttgtgaaag acttcttaga
      181 aaaagaaaac ttccaccttg tggatgttac agctgaaggt caagactttg ttgatgtgac
      241 tctcgctgtt gctgcagaag taaacaaaga agaacaaaac cttggtatcg tgattgatgc
      301 ttatggagct ggtccattta tggttgcaac taagatcaaa ggaatggttg ctgcagaagt
      361 ttctgatgaa cgttcagctt atatgactcg tggccacaac aactcacgta tgatcactat
      421 gggtgcacaa cttgttggtg atgaattggc taaaaacatc gctaaaggat ttgttaatgg
      481 taaatacgac ggtggtcgtc accaaatccg ggttgacatg ttgaacaaaa tgggctaatt
      541 agattatagt aagaaaggta agttaaaaat gagaattgca attggatgtg accacatcgt
      601 aacagatgaa aaaatggcgg tttcagaatt tttgaaatca aaaggatatg aagtcattga
      661 ctttggtaca tatgaccata cacgtactca ctacccaatc tttggtaaaa aagtagggga
      721 agctgtaact agcggtcaag ctgatcttgg agtatgtatc tgtggtactg gtgttggtat
      781 caacaacgct gtaaataaag ttccaggtgt tcgttctgcc ttggttcgtg atatgacaac
      841 agccctttat gctaaagaac aattgaacgc taacgttatt ggttttggtg gtaagattac
      901 tggtgaattg cttatgtgtg atatcatcga agctttcatc catgctgaat acaaaccaac
      961 tgaagaaaac aaaaaattga ttgcgaaaat tgaacatgtt gaaagtcaca atgctcaaca
     1021 aatagacgca aacttcttca ctgaattcct tgaaaaatgg gatcgcggag aataccacga
     1081 ctaagaggta acctatgatt ttaacagtta caatgaaccc atccattgat atttcctatc
     1141 ccttggatga gttgaagatt gatactgtca atcgtgtggt ggatgtaacc aaaacggctg
     1201 gtggtaaggg actcaatgtt acccgagtac tttcagaatt tggcgattct gttcttgcta
     1261 ctggtttagt gggtggcaaa cttggtgagt ttttggttga acatatcgat aatcaagtaa
     1321 agaaagattt cttctcaatt cagggagaaa ctcgtaactg tatcgctatt ctccacggag
     1381 acaaccaaac agaagttctt gaaaaaggtc ctgaagtatt ggaacaggaa ggtcaagact
     1441 ttttggaaca tttcaaaaaa ctcttggagt cagttgaagt agtggccatc tcaggtagtc
     1501 tgccagctgg ccttccagtt gattactatg cgagcttggt agaacttgct aatcaagctg
     1561 gcaagcctgt agtcttggac tgctcaggtg cagcacttca ggctgttctt gaatcacccc
     1621 ataaaccaac agtcatcaaa ccaaataatg aagaattgtc tcagcttctt ggaagagaag
     1681 tttctgagga tttggatgaa ttaaaagaag tacttcaaga acctttgttt gcagggattg
     1741 aatggattat cgtttcactt ggtgccaacg gtacttttgc caaacatggt gacactttct
     1801 acaaggtaga tattcctaga attcaggtgg taaatcctgt tggatctgga gactctactg
     1861 tggcaggaat ttcttcagga cttcttcaca aagaatcgga tgcagaatta ctcatcaagg
     1921 caaatgtcct tggtatgctc aatgctcaag aaaaaatgac tggtcatgtc aacatggcca
     1981 actatcaagt tctatatgat caattaatag taaaagaggt ataaaatggc tttaacagaa
     2041 caaaaacgtg tacgcttaga aaaactttct gatgaaaatg gtatcatctc agctcttgca
     2101 tttgaccaac gtggtgcttt gaaacgcctc atggctcaac accaaacaga agaaccaact
     2161 gtggcccaaa tggaagaact taaagtcttg gtagcagatg aattgactaa atacgcttca
     2221 tctatgcttc ttgaccctga gtatggactt ccagcaacta aagctcttga tgaaaaagct
     2281 ggtcttctcc ttgcttatga aaaaacaggt tatgacacaa caagcacaaa acgcttgcca
     2341 gactgcttgg atgtctggtc tgcaaaacgt attaaagaag aaggtgcaga tgcagttaaa
     2401 ttcttgcttt actatgatgt agatagctca gacgaactca atcaagaaaa acaagcctac
     2461 atcgaacgca tcggttctga gtgtgtggct gaagatatcc cattcttcct tgaaattctt
     2521 gcttacgatg aaaaaattgc ggatgcaggt tctgtagaat acgcgaaagt aaaaccacac
     2581 aaagttatcg gcgctatgaa agtcttttca gacccacgct ttaacattga tgtcttgaaa
     2641 gttgaagttc ctgttaacat taaatatgtt gaaggcttcg ctgaaggtga agtagtttat
     2701 acacgtgaag aagcagcagc cttcttcaaa gcgcaagatg aagcaacgaa cttgccatac
     2761 atctacttga gtgctggtgt atcagctaaa ctcttccaag atactcttgt atttgctcat
     2821 gaatcaggtg caaactttaa cggagttctt tgtggccgtg caacatgggc tggatcagtt
     2881 gaagcataca tcaaagatgg tgaaacagca gctcgcgaat ggcttcgcac aactggattt
     2941 gaaaacattg atgagctcaa caaagttctt caaacaacag caacttcatg gaaagaacgt
     3001 gtgtaagaaa gtcctcctag tttaggaaca tgaatctaaa aaaatttaaa aaaagttgta
     3061 tgtaaaggtt tacaaaataa cttacttgtg ctatacttaa atcacaagtt aatacaaggt
     3121 gagtgttact aagtaatatt aggcatgatc acaggtgaat tagaaatcag ctgattttct
     3181 agttcatttg tggtcatttt ttgtactcat atacctttaa gatataaaag gaggttgaca
     3241 tgtatcgaat tctaaatcca atgaatcaca atgtctcgct tgtcagaaat gataagggag
     3301 aagaggtgat tgtaattggt aaggggattg cattcggaaa gaagaagggg gatttgattg
     3361 ctgaaaatca ggttgagaaa atctttcgga tgaagaccga agagtccaga gaaaacttta
     3421 tggctcttct caaagatgtt ccgcttgatt ttatcacagt gacctatgaa atcattgata
     3481 agctatcaaa gaaatatcat tatccgattc aagagtatct ctatgtaacc ttgacagatc
     3541 atatttactg ttcttatcaa gctctaactc aaggaaggta caaggatagt aatctgccag
     3601 atatttctgc taagtatcct gtcgcttttc aaatcgcaaa tgaagctttt gaaatttact
     3661 gtcagaagct agcagatcat tttcctgagg acgaaattat tcggattgct tatcatttca
     3721 ttaatgctga aggtgaaaat gaagtggaac ttgtggagtc gattgataag aggaaagaaa
     3781 ttctcaggaa tgttgaagaa gttttaacgg actatgcaat tcaacgaact aaaaagaata
     3841 accatttcta tgatcgcttt atgatccatt tgaattattt cttggattat ttagacagat
     3901 ctagagatga taaccaatca cttctggata tggaagatca tattaaacaa tcctatccaa
     3961 aagccttcga gattggttcc aagatctatg atgtgattac gcaacatacg ggtcttgatt
     4021 tgtataaaag tgaacgagtt tatctagttc tacatatcca acgtttattg tcataaaaat
     4081 ttatttaaaa ctatataagg agaattctat catgaataga gaagaagtaa cattgttagg
     4141 ttttgaaatc gtagcctatg ctggcgatgc tcgttcaaaa ctattggaag ccttgaaggc
     4201 tgctgaagct ggtgattttg aaaaagcgga cgctctggta gaggaagctg gtagctgtat
     4261 tgcagaggct caccacgcgc aaacaagtct attgactaag gaagcttctg gtgaggactt
     4321 agcgtatagt gtgaccatga tgcatggtca agatcacttg atgacaacta tcttgttaaa
     4381 agacctaatg caccatttaa ttgaacttta caagagagga gttaaataaa atggataagt
     4441 taataa
//
LOCUS       DAVMGZ010000126         4414 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000126 DAVMGZ010000000
VERSION     DAVMGZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.127"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(280..831)
                     /locus_tag="VYQ93_001761"
     CDS             complement(280..831)
                     /locus_tag="VYQ93_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3336406.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1181..2005)
                     /gene="nadE"
                     /locus_tag="VYQ93_001762"
     CDS             complement(1181..2005)
                     /gene="nadE"
                     /locus_tag="VYQ93_001762"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV3336407.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(2002..3462)
                     /locus_tag="VYQ93_001763"
     CDS             complement(2002..3462)
                     /locus_tag="VYQ93_001763"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV3336408.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3578..4066)
                     /locus_tag="VYQ93_001764"
     CDS             complement(3578..4066)
                     /locus_tag="VYQ93_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336409.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4056..4265)
                     /locus_tag="VYQ93_001765"
     CDS             complement(4056..4265)
                     /locus_tag="VYQ93_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3336410.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1266 a         1034 c          820 g         1294 t
ORIGIN      
        1 aactcgatag aggattctat tttaaattta acaacagcta ggattacact ataaaaagta
       61 cattaccacc tgttcggctg gagaatctag tcgattagct ccctcttagt tacaagcttc
      121 aagcgatttt ttaactaaca ctaaaccaga ttaaagaagg aactagaaca tctgtcttgt
      181 actaatcaat atgctatttt aaagtttcaa ataaacttgg aatgctaatt attatattta
      241 caataaatag ttgacttcga tagaaacaga aaatagaatt caacattctt ccctacttat
      301 accataataa ataagatccg caagatagcc ttttctctct acaccattaa caatggtttg
      361 tagataggac attccagcct tttccatgac acgacctgaa gctgggttga gactggcata
      421 acgtgctctg accttttgaa aacctgcttg agtaaaacaa aagtccaaga tagctttcaa
      481 agtctctgtc atcataccat ttccccagta agccttgcct aacacatagc caatttcaca
      541 gcttaaatca gcctcgtcta tcttaacaat gctgatatct cctattactt gctctgggtt
      601 ttcttttaga caaatggccc atttataata gttgagatta gtataggaag caacccaatt
      661 gcaaatcgag tttcgagtga tttcgacatc aggatgggga tcccaggtaa cataggtcag
      721 attctcagcg gatgaagccc aattttgaaa catggcttct gcatcactct ccacaaatct
      781 tcttaaaatc aaacgttctg tctgtaatat ttgcgtaccg atagatttca tgatgctacc
      841 tcacttcctt atttctatac tcgtagtaaa aaggctcaac tcagtaacag ctagaaaacg
      901 agtttgtctt ctaatttctt gacgcagttg ctgcctggtt tgaaatgcgc tctagcaagc
      961 tttttcaaac ctagtcatcg ttgcaggggt aagacgatga aatcgaactc ttcgagttac
     1021 tgaatctgcc tcattttctt atttctaaac tcggggttca agatccccca gaccttctct
     1081 cactttctta tttcaagatg agaggctgaa aacctccagt ggaggttttt cttgcttttt
     1141 aatttcaggg caagaagcta aaaaggtccc ccggaccttt ttactcccaa aagtcatcaa
     1201 atacggtgat gggtaagtgg cgtttgtgtt ggcctttgtg ccaccagttt tcaatggtcg
     1261 cttgagcttc tgggctgatt gttttgcctt ctaggtagtc gtcaatctct gcgtaggtga
     1321 ctccaagtgc gacttcgtca gctaggcctg gtttatcttc ttctaggtct gccgttggga
     1381 ttttttcata aagggctggc tctgcgccaa gtttctgcaa gagctgtttt ccttggcgtt
     1441 tattgaggcg gtaaagaggg agaatatccg caccgccgtc accaaacttg gtaaagaaac
     1501 ctgtgatatt ttccgcggcg tggtctgttc caatgaccgc tccgctatgg gaaccagcaa
     1561 gggcatactg agcaatcata cggcaacgtg ccttgatatt ccccttgttg aagtctgaaa
     1621 caggactacc tgtcgcttca actgcagctg tcatggcatc agctgattcc ttgatattca
     1681 caaccaagct gacatctggc tggatgaagg ctagggcttt ttgagcatct gcttcatcag
     1741 cttgcactcc gtatggcagg cggacagcga taaatttgta gctatcgtct cccgtttcag
     1801 ctcgcagttc ttccatagct aattgcgcca aacgtcctgc caaggttgag tcttgtcccc
     1861 cagaaatccc tagtacaaag gtttttagga agggatgttt tttcagatat ctttttaaga
     1921 aatcaataga acgacggatt tcttcctggg catcaatcac tggtttgaca cccagctctt
     1981 ggataatcgt ttcttgcaaa ctcattcttc ttctccttca ccaagggctt ccttgcgcat
     2041 cttatcaatc aagtccatct tatcttgcca tacatcacgc gccaaatcca ctggatagtg
     2101 ctgcggattg agcacacgct tatactcatc ccacaacttg tcaaattcct tacgggcata
     2161 atcctgaatg tcagtcaaac taggcaagtt gtaaactaat attccttctt tgaagatatc
     2221 caccaagaga ggaacggcat caaaattacg aaccgtcttc ttgatgtatg tataggtcgg
     2281 atggaacatc ttgatttctg tcatgtcgct aatatccaca ccatcataag tgatatagtc
     2341 gccttctgac ttgccttttt cacgactggt aatgcgccac acctgcttct tacctggcgt
     2401 agaaactttt tcagcattat tagacagctt aatcgtattg cgcatctgac cagtttcatc
     2461 ttcgattgca acaatcttgt aaaccgcccc aagagccggc tggtcatagg ctgtaatcag
     2521 cttggtaccc acaccccaga catcaatctt ggccttttgc atcttgaggt taaggatggt
     2581 attttcatct agatcattag aagcataaat cttagcctct gtaaatccag cctcatccag
     2641 ttgctgacgg actttcttag aaatgtaggc aatatcccca gagtcaatcc gcacacccat
     2701 aaagttaatc tgatcaccca gctcacgcgc cacctgaatg gcagctggta caccgatgcg
     2761 aagggtgtca taggtatcca caagaaagac acaatttttg tgggtcgcag cgtaagcctt
     2821 gaaagcttca tagtcattgc cataaacctg taccaaggca tgggcatggg ttcccaaaac
     2881 aggaatgtca aagagcttac ccgcacgcac gttgctagtt ccattggcgc caccaatcac
     2941 agctgcgcgt gttccccaga tggccgcatc catttcttga gcccgacgtg tcccaaactc
     3001 catcaagggt tcatcttcga taaccgaacg aatacgagct gccttcgtcg ccaccaaggt
     3061 ctggtagttg acgatgttca aaagagccgt ttcgaccaac tgacattggg ctagaggtcc
     3121 ttccacctgc acaatcggtt cattagcaaa aaccaaatcc ccttcttggg cagaacgaac
     3181 ggtcaactcc aacttgaaat tgcgaaggta atccaagaac gccccatgat aaccaagcga
     3241 ctccaaatag gctatatcac tatctgaaaa acgcaagtct tcaagatagt tcacaattct
     3301 ttccaaacct gcaaaaaccg catagccgtt cttaaaaggc tgttggcgga aatacacctc
     3361 aaagaccgcc ttcttattgt gaatcccttg gtcaaagtaa acctgcatca tgttgatctg
     3421 gtacaagtcc gtgtgcaatg tcaaactatc atctggatac atacttttcc tacttcctta
     3481 gctagaaacc catgaaaatt ttcaagaact ttcatgtatt ccaataaatt agtactatta
     3541 tatcacattt tagctggatt gagaaaagag taacaagcta ttctccactc tccaattcat
     3601 ccatatcttg ttcaaatttt ttctgagccc attcgccata gctcttaaga ccaagattgc
     3661 caataaagat ccacggaagg taaatgatat aagtaatgac ccaagcagac aggtatttaa
     3721 aattcaaagg attgtgctga taaatttcta tgttgaattg ataattctgc aacatcaaaa
     3781 gagccgtaat agccaaggtt aggaaaaaac aacccaaaat cgtaaaatga aaacgactat
     3841 agtaggtcac tcccagataa cgggcacgat tgaaaaagta aaatgtccct atgatgataa
     3901 cgattagcag catattagaa ttaaaaaggc ttggtgctaa tactgaaatg atataagata
     3961 ggagcgacaa agcgatgcag atatagaaac tttcagagcc cgctttattg aacagttgtt
     4021 cttctctttc gtctagtaat tgataataat aaaatctatt tttcatcttc ttcctcccaa
     4081 aatagttggt ctagggtttt ccctaaacat ctgcaaatgg actggcagag cgagagactg
     4141 ggattgtatt tccccgcttc tatcaaacca atagtctggc gcgtcacccc gacagcctct
     4201 gccagttgac cttgtgttaa atcacgctct acccgagcta attttaattt taaattttta
     4261 gccaccttcg tcctccttat agttttaata ctcatctact cttaaaaaat ccaaaaccaa
     4321 cacaagctat cagctattta ttataacccc attaatttgc actccagtat tatcttcagc
     4381 cgaaaatcag tattttaggt tgtttgaaat aaac
//
LOCUS       DAVMGZ010000127         4394 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000127 DAVMGZ010000000
VERSION     DAVMGZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.128"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(36..794)
                     /locus_tag="VYQ93_001766"
     CDS             complement(36..794)
                     /locus_tag="VYQ93_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV3336411.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            1002..1172
                     /locus_tag="VYQ93_001767"
     CDS             1002..1172
                     /locus_tag="VYQ93_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336412.1"
                     /translation="MEIKEQTRKLAVSYSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEYKEHHNIDV"
     gene            complement(1295..2425)
                     /gene="ugpC"
                     /locus_tag="VYQ93_001768"
     CDS             complement(1295..2425)
                     /gene="ugpC"
                     /locus_tag="VYQ93_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV3336413.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(2708..2793)
                     /locus_tag="VYQ93_001769"
     tRNA            complement(2708..2793)
                     /locus_tag="VYQ93_001769"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2757..2759),aa:Leu,seq:aag)
     gene            complement(2875..3450)
                     /locus_tag="VYQ93_001770"
     CDS             complement(2875..3450)
                     /locus_tag="VYQ93_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV3336414.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGNLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            complement(3450..4238)
                     /gene="codY"
                     /locus_tag="VYQ93_001771"
     CDS             complement(3450..4238)
                     /gene="codY"
                     /locus_tag="VYQ93_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV3336415.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
BASE COUNT         1338 a          940 c          724 g         1392 t
ORIGIN      
        1 gaaagaaaaa aggaaaagtt aacttcaata attatctaac gttttgacga tttataacta
       61 gccatcgcaa taaaacccaa tttctgttta ttcttagcaa acattttata catagttaaa
      121 aactgctttc tattctcctt tttacaagca tttacacaaa ttttcaaagt tcctagcaaa
      181 ccttcgtcat aaatcatacc cgataatttc attaatgtca tttcaccagt caatactttc
      241 acatcacaat aacctgattc tatcatcacc tgttcccaac catcttgagt taaaggacct
      301 acatttacat gaattgcttg tgataattcc tgtctgatag actctttagc ttccttaaga
      361 agcacatcat gcgtcaatag aagacctcca ggttttaata cccttagata ttccattaca
      421 cattttttct tagcttgatc ggcttgcata gtcagcatag cttcatttat aacaatatca
      481 aaactagcat cttgataagg aagtttcatt gcatttgctc tttcaaaact gattaaatga
      541 gcaacacctg ccgttccagc agatttttta gccacttcta aagcttgagc atccatatca
      601 acagcagtta tcttgcaacc aaaacgctgt gccaactcaa ttgctgtagt tcccctatta
      661 cacgcaacct ctagtattct cttttctttt gaaaatcctc cttctgcaat taaccaatct
      721 gtggcacgct ttccacctgg acgtaagcgt tttttcccca attttgctaa aaacttatga
      781 cctgcttctg acatttgaac acctctgttt tttcttcatt ataacacaaa atgatattat
      841 ctgacaattc taatctatta tttgataaac tagaatcaaa agcatacaag tcaccataac
      901 tatagcataa atttattatt acctctctag ttcgaattca attgctcacg tagtttacgc
      961 atatagcttc acttgtagat ggtacccaca aataatactc aatggaaatc aaagagcaaa
     1021 ctaggaaact agccgtaagc tactcaaagt acagctttga ggttgcagat aaaactgacg
     1081 aagtcagtaa tcatacgtac ggcaaggcga cgttgacgtg gtttgaagag attttcgaag
     1141 agtataaaga gcatcacaat atagatgtat aatatgactt ccctagcaaa aaggagagaa
     1201 gcattttgtt tctctcccta gattaactga tatctctcca ctacaattga taaagagata
     1261 tcttttcttg tagtgctttg aattttattt attcttagta gattgttttt tcagtttcta
     1321 catcgaagaa gtgtgctttg ttcaagtcaa atccaagctc aactgttgca cctgtttgca
     1381 agtagtcacg agcatcaact tttgcaacaa actcgtcttt accaacttgg cagtaaaggt
     1441 gagattctga accaagcagt tctgatacag agatagtcgc ttttacaaca cagtctggga
     1501 atgtttcaag gaaagcaggt tctgcattca cgtcttctgg acggatacca aagatcaatt
     1561 cttttccttc gtagcctttt tcacgaagaa ctttcaatgc tccttctggc actttcaaac
     1621 ggaaaccgtc agaaacaatt tcgctaccaa ccaatttcac gttgatgaag ttcatagctg
     1681 ggcttccgat gaatcctgca acgaatttgt taactggatt tttgtaaact tcttgaggag
     1741 taccgatttg ttctacacgt ccgatagtac ctgtaccagc agggttctta gtagctgaca
     1801 taataacgat acggtctgca agtgtcatcg cttctgtttg gtcgtgagtt acatagatag
     1861 ttgtagctcc gatacgacgg tgaattttag cgatttcagc acgcattgat acacgaagtt
     1921 tggcatccaa gtttgacaaa ggttcgtcca tcaagaatac tttcgcatca cggacaatcg
     1981 cacgccccat ggcaacacgt tgacgttgac cacctgaaag gtcagccggt ttacgttcca
     2041 agaattcttt caatccaagt atttcagctg cttcttgaac acgtttgtta atgtcttctt
     2101 tgctgtattt acgcaatttc aaaccgaaag ccatgttgtc ataaacagtc atgtgtgggt
     2161 aaagagcgta gttttggaat accatggcga tatcacggtc ttttggagct acgtcgttga
     2221 caaccacgcc atcgatagat gcagtacctt ctgtgatgtc ttcaagacca gcaatcatac
     2281 ggagtgtagt tgatttacca catcctgaag gtcctacgaa aacgataaat tctttatctt
     2341 tgatgttcaa gttgaaatct tcaactgaat agtgttcgct gtttggatat tttttgtaaa
     2401 tatttttaag attcaattct accatgaggt gaactccttt tgtcttttga taattatatt
     2461 atagatgaaa acgctttata tttctatggc aagatgacca aaaaaataaa aaagttctgt
     2521 gcaacttgca caaaacttta gagaatatcg gagaatttga agaagataat ctttattatt
     2581 cttctatgac aaaagcagaa acaaggatca gttttcccat attttcgctg attctccact
     2641 acatttgact ggattctaat tttttagaga aatacaaaag agctagcttt agctaactct
     2701 tttcctatgc ggagagaggg acttgaaccc tcacgaccta aagcggtcac aggatcctta
     2761 gtcctgcgcg tctgccaatt ccgccatccc cgcatcgatt actttactag tatatcaact
     2821 tttgggatgc ttgtcaacac tttttttcaa attttttcat tttcaccaac caggttactc
     2881 agaaagttca tttagatttt catctactaa cttagctccg agtgtatttt tgaaatgacc
     2941 tagggcaaat tgatgatttt caggccagat ggaagcaaca gctggtttaa caatctcgat
     3001 gtcatatcct agattatagg catctatagc tgtatgtagg acacagatat ccgtcaagac
     3061 acctgttaag ataacggtag acactctacg ctctctcaaa cgaatatcta ggtcagtccc
     3121 tgaaaaagct gagtaatggc gtttatccat ccaaaagaca cgactgtctg aaccatgctc
     3181 ttgataaaag atccccaaat ttccatataa attccgtcca ctcgtcccaa tcagattatg
     3241 aggaggaaat aacttacttt ccggatggaa acaatcgttt tcttcatgag catcaatagt
     3301 aaagaagata taatctcctc gttcaaaagc taatcgagtt accttgctga tggcatccga
     3361 aatcgcctga gctggagcac ctgctgtcag cttcccacta tcagcaacaa aatcttctgt
     3421 ataatcaatc gaaattaaag cctttgtcat tagtaatctc ttttcttcac ttcttcaaaa
     3481 atatctgaaa tcaagacctt aagataggtt cccttcattc caagtgagcg actttcaata
     3541 atccccgcag actcaagttt acgaagagca ttgacaatca cagagcgagt gattccgata
     3601 cgatctgcaa tcactgacgc agtcaacttc ccttcatttc catttaattc ccctaaaatt
     3661 gctgaaacag cacggagttc ggagtaagaa agggtattga ccgccatggt gacagcagta
     3721 cgacgacgaa tatttttctc atcttcttca cgttggaagt taagaagctg aatcccaaca
     3781 acggtactgg caatctcaac aagaaccaag tcctcatctt cgaatttttt atcattacgc
     3841 caaataatca aagaaccaag gcgaatcccc gatacatgaa tcggtgcaat agtcgtcaag
     3901 ccatctggaa aatcatctct actctcaaca gggaaaatac tcatatcatg ctcaacaggc
     3961 aagtttgctt ctgtttcgta aatcatatta gccccttgaa cgtagtcatc tgggaaaatc
     4021 ttagtttgga agaattgctc tacgcgatct gtatttgttt tataacgcat aaaatagcca
     4081 agcagacgtc ccttactatt gataatgcag gcattgcaat gaataatatc cgctaactga
     4141 cgcgtaatag cgttgtaagg gagctcatcc tgcaactgct cctctgagcg tttcaaaatt
     4201 gatgtaattt ttctagtttt ttctaataaa tgtgccattt ttcacctcga atttaatcgc
     4261 tatcattata acataaaaac gtctcttttt caataattat ctgaaaattc cttattgact
     4321 tgcattgact tacaatttaa ttaaaaacca gaatattttt aattaaattg ttccttttct
     4381 attgacaagt cgcc
//
LOCUS       DAVMGZ010000128         4285 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000128 DAVMGZ010000000
VERSION     DAVMGZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.129"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            25..822
                     /locus_tag="VYQ93_001772"
     CDS             25..822
                     /locus_tag="VYQ93_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV3336416.1"
                     /translation="MKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPVVYFHDGQNV
                     FNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQESPIPEQQF
                     GGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHPDVFTKVIAL
                     SGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCTGLGAWEQDG
                     LPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            835..2001
                     /locus_tag="VYQ93_001773"
     CDS             835..2001
                     /locus_tag="VYQ93_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV3336417.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(2492..4162)
                     /locus_tag="VYQ93_001774"
     CDS             complement(2492..4162)
                     /locus_tag="VYQ93_001774"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV3336418.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
BASE COUNT         1269 a          904 c          877 g         1235 t
ORIGIN      
        1 aatcaatcct acttttatct aaaaatgaaa gaacacaaac tcaaggttcc ttatacaggt
       61 aaggagcgcc gtgtacgtat tcttcttcct aaagattatg agaaagatac agaccgttcc
      121 tatcctgttg tatactttca tgacgggcaa aatgttttta atagcaaaga gtctttcatt
      181 ggacattcat ggaagattat cccagctatc aaacgaaatc cggatatcag tcgcatgatt
      241 gtcgttgcta ttgacaatga tggtatgggg cggatgaatg agtatgcggc ttggaagttc
      301 caagaatctc ctatcccaga gcagcagttt ggtggtaagg gtgtggagta tgctgagttt
      361 gtcatggagg tggtcaagcc ttttatcaag cacaaaacag gttggtttga tggcatgatg
      421 acgacaggtt gctctatggg agcctatcat gcactcaatt tcttcctcca gcatccagat
      481 gtctttacca aagtgattgc tctcagtggt gtttacgacg cacgtttctt tgtcggcgat
      541 tactacaacg atgatgctat ttaccaaaac tcgccagtag attatatttg gaaccaaaac
      601 gatggctggt ttattgaccg ttaccgtcag gcagagattg tgctgtgtac ggggcttgga
      661 gcctgggaac aagatggttt accatccttt tacaagctca aagaagcctt tgaccagaaa
      721 caaattccag cctggtttgc tgaatgggga catgatgtcg cccacgactg ggaatggtgg
      781 cgtaaacaaa tgccttattt cctcggtaat ctctatttat aaaaggagtt acctatgaat
      841 taccttgtta tttctcccta ctatccacaa aactttcaac agtttaccat cgaactagct
      901 aataaaggca tcacagtctt gggaattggt caagagtctt acgagcaatt ggatgagccc
      961 ttgcgcaata gcttgaccga gtattttcgt gttgataatc ttgagaacat agatgaagtc
     1021 aaacgtgcag ttgcttttct cttttataaa catggtccaa ttgaccgcat cgagtctcac
     1081 aatgaatact ggcttgagct agacgcaaca ctcagagaac aattcaatgt ttttggtgcc
     1141 aaaccagagg atctcaaaaa gacgaaatat aagtctgaaa tgaagaaact tttcaaaaaa
     1201 gcaggtgttc ctgtggtacc tggagctgtt atcaagacgg aagcagatgt tgatcaagca
     1261 gtgaaagaaa tcggtcttcc aatgattgcc aaacctgata atggagtggg agcagccgca
     1321 acctttaaac ttgagacaga agacgatatc aatcacttca agcaagaatg ggaccattca
     1381 accctttatt tctttgaaaa atttgtcact tccagcgaaa tctgtacctt tgacgggctc
     1441 gtggacaagg atggaaagat tgtcttctca acaacctttg actacgccta tacaccgctt
     1501 gacctcatga tttataagat ggacaattct tattatgtgc tcaaggatat ggatcctaaa
     1561 ctgcgcaagt atggggaagc aattgtcaaa gaatttggta tgaaagaacg gtttttccat
     1621 attgagttct tccgtgaggg ggacgattat attaccatcg agtacaataa ccgccctgca
     1681 ggtggtttta ccattgatgt ttataacttt gctcattcct tggaccttta tcgtggctat
     1741 gcagctattg tcgcaggaga ggagttcccg gcgtcagact ttgaaactca gtattgtttg
     1801 gctacttctc gccgtgcaaa tgctcactat gtttattcag aagaggattt gcttgccaaa
     1861 tatagccagc agttcaaggt taaaaaagtc atgccagctg ccttcgcgga acttcaagga
     1921 gattacctgt atatgctgac aactccgagt cgacaagaaa tggatcagat gattgctgat
     1981 ttcggacaac gtcaagaata agaactatcg gattaaggaa attcactccc ttaatccttt
     2041 tgttttgttt ggtaaaaaat aagagcatcc taacaaggta gctatcataa aacttgttcg
     2101 ataactattt gaagcaggat taggtggcta gaaattgaat tttaacattt caatttagtc
     2161 atagtattgt gttttcgtat ccttaaatca gctaaaagga tccatgacga cacctatacg
     2221 atatagtttt caggatacca aaccagtcta ttaatactca atgaaaatca aagagcaaac
     2281 taggaagcta gccgcaggtt gctcaaaaca ctgttttgag gttgtggata gaactgacag
     2341 agtcagtatc atatacctac ggcaaggtga agctgacgta gtttgaaaag attttcgaag
     2401 agtataaatc ttattgatga actgcttgca gtctgagaaa aaatgagctt ggatattatt
     2461 tccaaactca cttaaagtca atttcaatcc actagaacaa gcctagtaca gttccatcgc
     2521 tttcaacatc catgttgaga gctgctggac gttttggaag acctggcatg gtcataacat
     2581 caccagttaa ggcaacgatg aagcctgcac ctaattttgg taccaattca cgaatggtaa
     2641 tttcaaagtt ttctggtgct ccaagcgcat ttggattgtc tgagaaactg tattgagttt
     2701 tagccataca aattggcaat ttgtcccaac cgttttgaac gatttgagca atttgtgttt
     2761 gagctttctt ctcaaagttc actttgctac cacgatagat ttcagtgaca attttttcaa
     2821 tcttttcttg gacagaaagg tcattatcgt acaaacgttt atagttagct ggattttcag
     2881 caattgtctt aacaactgtt tcggcaagtg ctactccacc ttctgctcca tcagcccaga
     2941 cactagccaa ttcaactggt acatcgattg aggcacagag ttcttttaag actgcaattt
     3001 cagcttctgt atcagataca aattcgttaa tagctacaac tgctggaata ccgaacttac
     3061 ggatattttc aacgtggcgt ttcaagttag caaaacctgc acgaactgcc tctacatttt
     3121 cttcagtcag agcgtcttta gccacaccac cattcatctt aagggcacga agggttgcga
     3181 caataacaac tgcatctgga gatgttggca agtttggtgt cttgatatca aggaatttct
     3241 cagcaccaag atccgcacca aaaccagctt cagtaacagt gtaatcagcc aagtgaaggg
     3301 ctgttgtcgt cgccaaaaca gagttacagc catgagcgat attggcaaat ggaccaccat
     3361 gtacaaaggc aggtgtaccg taaattgtct gaaccaagtt tggcttaata gcatccttca
     3421 aaatcaaagc caaggcaccc tcaacctgca aatcacctac agaaacaggc gtacggtcat
     3481 agcgataacc aataacgata ttcgccaaac gacgtttcaa gtcctcgatg tccgttgcca
     3541 agcaaagaat tgccatgatt tctgaagcaa ctgtaatatc aaaaccatcc tcacgtggaa
     3601 taccgtttag aggaccacca agaccaacag tcacatggcg gagcgcacgg tcgttcaagt
     3661 ccacaacgcg tttccagagg atacgacgtt gatcaatttc cagctcattc ccttggtgca
     3721 agtggttgtc aatcaaggca gaaagggcat tgttggcagt tgtaatagca tgcatatctc
     3781 cagtaaagtg gaggttgatg tcttccattg gcagaacttg tgcataccca ccaccagcag
     3841 caccaccctt gatccccatg actggaccaa gagacggttc gcggatagca atcatggttt
     3901 tcttgccaat cttgttcaag gcatccgcaa gaccaatggt aagcgtcgac tttccttcac
     3961 ctgcaggtgt tgggttgatg gcagtaacca agatcaattt accgactgga ttgctctcaa
     4021 ctgcacgaat tttatcaaag ctgagtttag ccttgtactt tccgtacaac tccaaatcgt
     4081 cgtaagaaat accaagtttc tctacaacat caacaattgg cttcaactca atactctgtg
     4141 cgatttcaat atctgttttc attcaaaatt cctctaacct cttatatgat aattcattat
     4201 atcacaaaac aagattttta acatcctaaa actctctaaa cgttcgtaaa tatctctgtt
     4261 tttaagactt ttagagtcct ttctt
//
LOCUS       DAVMGZ010000129         4279 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000129 DAVMGZ010000000
VERSION     DAVMGZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.130"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            8..328
                     /locus_tag="VYQ93_001775"
     CDS             8..328
                     /locus_tag="VYQ93_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336419.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(359..697)
                     /locus_tag="VYQ93_001776"
     CDS             complement(359..697)
                     /locus_tag="VYQ93_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336420.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(709..1428)
                     /gene="trmD"
                     /locus_tag="VYQ93_001777"
     CDS             complement(709..1428)
                     /gene="trmD"
                     /locus_tag="VYQ93_001777"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV3336421.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(1418..1936)
                     /gene="rimM"
                     /locus_tag="VYQ93_001778"
     CDS             complement(1418..1936)
                     /gene="rimM"
                     /locus_tag="VYQ93_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV3336422.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(2006..2755)
                     /locus_tag="VYQ93_001779"
     CDS             complement(2006..2755)
                     /locus_tag="VYQ93_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV3336423.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGRNHHYLQWSE"
     gene            complement(2859..3063)
                     /locus_tag="VYQ93_001780"
                     /pseudo
     CDS             complement(2859..3063)
                     /locus_tag="VYQ93_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            complement(3331..3570)
                     /locus_tag="VYQ93_001781"
     CDS             complement(3331..3570)
                     /locus_tag="VYQ93_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV3336424.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(3590..3862)
                     /gene="rpsP"
                     /locus_tag="VYQ93_001782"
     CDS             complement(3590..3862)
                     /gene="rpsP"
                     /locus_tag="VYQ93_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV3336425.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            4032..4241
                     /locus_tag="VYQ93_001783"
     CDS             4032..4241
                     /locus_tag="VYQ93_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336426.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
BASE COUNT         1254 a          913 c          712 g         1400 t
ORIGIN      
        1 ggccaatatg agacataaat tccagcaagt tctagataaa atacatgatt ttttaaatgg
       61 acatgaccaa cctgaccaga ctgaaaccaa ctcccttaca gccactattg aagaggctat
      121 ccagaaacaa accgctgttc accttatctt gtctgagaca agttttacag gtgatatcat
      181 caaatatgat cagcaaggcc agcaaattat cgtgaaaaat tttgccaaaa atgtgagccg
      241 gattatccgt ataagcgata ttcaacgcct gcgatttgtc ccctcaactg tccaaacagc
      301 ccaaaaaaat agatttaaga aagagtgaga tgtagttgct tcatcccact cttttttctt
      361 agcgaatttg ttcaaaatgt aaatgaactg caatatgatc tccataacca cttctttcca
      421 agtcacgttg taaacgatag gaaatgtagt gttctgcaat ggtaatgtaa cctgcgccca
      481 ataaacgatg ttcaaccata gattgaatca tactgatagt cgcacgttcc accttggctt
      541 cttgtaaatc caaaactacc ttcttagtga cttgagcaag attttgacgc aaatcatctg
      601 tcaaaacata aacagtttgg gctgccttca agatggcttg gtaaatctta tctggattaa
      661 attcagcaat ttcgccatta cgtttgatta cttgcatagg tttctccttt attctttgtt
      721 ttctttgatt tctgccagca ttttttcttc ttctactgtc agttgataat gttcaagtaa
      781 atccggtctg cgctcgtagg ttttctttaa actctcgtac aatcgccact gacgaatctt
      841 ttcatggtgg ccactcatca atacatctgg cacgaccatg cctcgataat catagggacg
      901 tgtgtactga ggatattcta aaagacctga agaaaaacta tcatcttggt ggctagactc
      961 cttgccaatc acttctggaa tcaggcgaac tgtagcatca atcatggtca tagctgccaa
     1021 ttctccacca gtgaggacat agtcgcctag ggaaatctca tctgttacca aggtcttaat
     1081 gcgctcatca taaccctcat agtgcccaca gataaagatt agctcttcct cttgagccaa
     1141 atcttcagca taagcctgat caaactgctt tccagcagga tcgaggagaa taacgcgcgg
     1201 atttttcttt tcaatagcat caaaggaatc gaaaataggt tgtgctctga gcaacatgcc
     1261 ctgaccgcct ccgtagggct catcatctac atgacgggcc ttttcagcat tttctcgaaa
     1321 attatgatac tggatatcca agagcccttt ttctcgagcc tttccaacga ttgagtgctc
     1381 cagtggagaa aacatctctg gaaagagggt taaaatatca atcttcatcg tctaaccctt
     1441 ctaagatttc cacatcgacc cgtttatttg gaatatcaac attgagaacc actggtggga
     1501 tataaggtaa aagcaaatca cgtttgcctt ttcgtttgac cacccagaca tcattagcac
     1561 ctggttgcag gatttccttg atggttccaa ccaagctatc accctcatag acttccaaac
     1621 cgataatctc gtgatagtaa aattcaccat cgtctaggtc attcaaatct tcctcagcga
     1681 ccttgagact gtatcccttg tacttttcga tagtattgat atggtacata tctttgaatt
     1741 taataatgtc aaagttcttc tgtttacggt ggctagcgat ggtcactgtt tggacaaact
     1801 gatctttttc atcaaacaaa gccagctcag ctcctttttt aaaccgttct tctgcaaaat
     1861 ccgtcacaga caagactcgc atctcaccct gtaatccctg cgtattaacg attttcccaa
     1921 cattaaagta gttcatcttg tctcctgtaa tctccttttt tccatcttat tctgacaatt
     1981 ctcgaataat agccgcaatt ttttcctatt ctgaccattg taaataatgg tgattccttc
     2041 ctaaaatgag tttagtattg gaagtccaat attctgattc tctgtactct ttttctctat
     2101 aagtctgaca aaaaataaat acagggacat gagattctat cgacaaattc tcaaaatctt
     2161 cctcagtaat ctctccagat atctgaaatt ctggatcttg attttccaac tctaagcctt
     2221 tttcttgcat taattcccag atttttttat tcgtttcagg actaaatgtt gcttgagtta
     2281 agttcttaaa ataaagttca ggaccacact cgtcaatcag cctcatctgc tcttccattt
     2341 ctggataagg attttctgaa aaatcagcaa acatgacttt tttagttgtc ggttcaattg
     2401 ctactaaagt ctgacgctta attggtttct cgagtaattt gcaagctaaa attccactcc
     2461 aactatgtgc acaaagtata tattcagaaa ttcctaattc ttcaagtact tcataaaccg
     2521 catctgcaag attatctaga ttttttccag cttggtcatg aatcggactc ctacctgtgt
     2581 tcggaaaatc aattgtcaaa taaccaattg taggaggagg tttttcaagt ataagtgaaa
     2641 aattttcata acttggtagc aaacctgctc cgtttagaca aactagcact ttcttttgct
     2701 tttgataagt aacagagagg ctaccaattt ctgtagatac ttcaaacctc ttcataaaga
     2761 aatccactca ttctatataa tgaattatta aaaatcctta tcttttattt tatcacgttc
     2821 caaggatttt ctcaagttgg aggaagggga caacatctct actttccctt caaaaatcct
     2881 tccaaatcat gtttatgttg gtaattaacg gctgcggttt tgtctttctc aaagacagtc
     2941 ttggtaaggt caatatgatt aatagctacg attgcgacgg tgtagtaaat gatatcagcc
     3001 agttctctgg caagttcctc gttcgaatcc tatcccttct tttcgaccag agcgcctatt
     3061 caaaacctcg gctacttctc cgacttctcc actaacttca taaagagacc ttcatcagtc
     3121 cgagactgct gttaatgttc gagtaagtag tcttggaatt gcctaaacgt tcaatctttt
     3181 atagtatatt gaaactagaa tagtacacct ttacttctaa aacattgtta gaaatcgatt
     3241 tgactgtcct gatcgatttg tcctgttatt atttcatttt actatatctt ctattccaca
     3301 caaaaaagcg agacatccgt cccgcccttc ttatttttcg tcaataacga ttcttacttt
     3361 tttgtattca gttgggacag agtagacaat cgttcttatc gcagaaatag tgcgaccctt
     3421 acgaccgatt acacgaccca catcgctttg atcaagattc aaatgatatt ccaaaaattc
     3481 tggtgtatcc tcaatcttga tagttaaggc atctggttgt gaaattaagg gtttcacaat
     3541 cgcaataatg agattttcaa tcgtatccat ctgtcaacct actttaaact tattttgaaa
     3601 atttagaatc gtggaatttt ttcaatacgc cttcttttga aaggatgtta cgtactgtgt
     3661 ctgaaggttg agctccatta gccaaccatg caagaacgcg gtcttctttc aaagttactt
     3721 ggttttcagc aacaagtggg ttgtaagttc caactgtttc gatgaaacgt ccgtcacgtg
     3781 gtgaacgtga atctgctacg ttgatacggt agaaaggttt tttcttagaa cccatacgag
     3841 tcaaacggat tttaactgcc atttttaaag tctcatttct ttaatttttt atttcggtga
     3901 aatagctgag ctatctagca catgttctat tatagcagat ttctggcatg tgtcaagaaa
     3961 aaaacttgac agactataaa tttcttaaag tatttagaaa tttgttagaa taggaaataa
     4021 atgtttgaaa gatgctatcc gtgaatacta ttttagaaaa attttataaa gaacaccaag
     4081 tcaaaccctt catatcgcct gaacgagagt tggatacttg gcttctaagt cccaagcccg
     4141 ttcccaagcg aaatatggac ttattagtag atgattcact agcaggagat atcattttac
     4201 tatggagaat ccagtttggt acttttacca ctgaaacatg attttaaaaa ctcccattcg
     4261 aatatgattc aaaattcgc
//
LOCUS       DAVMGZ010000130         4215 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000130 DAVMGZ010000000
VERSION     DAVMGZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.131"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            659..1651
                     /locus_tag="VYQ93_001784"
     CDS             659..1651
                     /locus_tag="VYQ93_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3336427.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1653..2471
                     /locus_tag="VYQ93_001785"
     CDS             1653..2471
                     /locus_tag="VYQ93_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV3336428.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2473..3258
                     /locus_tag="VYQ93_001786"
     CDS             2473..3258
                     /locus_tag="VYQ93_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3336429.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3356..3574
                     /locus_tag="VYQ93_001787"
     CDS             3356..3574
                     /locus_tag="VYQ93_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV3336430.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3559..3720
                     /locus_tag="VYQ93_001788"
     CDS             3559..3720
                     /locus_tag="VYQ93_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV3336431.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNP"
     gene            complement(4032..>4215)
                     /locus_tag="VYQ93_001789"
     CDS             complement(4032..>4215)
                     /locus_tag="VYQ93_001789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336432.1"
                     /translation="SKHCFEVVDGTDVVSSKHCFEVVDRTDEVSNHTHGKATLTWFEE
                     IFYEYKSKTFVNRDTL"
BASE COUNT         1167 a          734 c          913 g         1401 t
ORIGIN      
        1 tacaaacaga atatgaaagc tttttatatc actattgaaa aatttatacg agatgatgaa
       61 agccttaagt gttattttac aaaggttatt tcaagtcgtt ccaaggtaac aagtctagat
      121 cagattgaag ctgataaaac gatacaaaga aaatattcaa gtgagctaaa aaaatttatt
      181 ggattttata atgagattat ttgtgaggaa aatagtttcc tacatgtacg aaagaggtgg
      241 tcgagttggt ttaggtagtc ggtgcgtgag ttgataattc tcagggtatg gacttctttt
      301 tcatgaatga ggtaaaagag caggtattgt ttagagacaa tcattctgag catattttct
      361 ggatagaggg agtatccgat tttatgatca aagttaatac cgccctctgg tgagaagatg
      421 agtaggttgg taatttaaac tattaaacag aatttttgat taaaagtatt atttcatgag
      481 agaaatccta atttcacaat ctataggcaa acgcttgcat ttcgtttttt attggactat
      541 aataggttgg tataaagcct tctgtagtaa taaaatgtag aaggtgtaga aagtaaggat
      601 ttagaatatt tgtagttaaa aacacaatgt tgctattcct tacgataggg agatagatat
      661 ggcaatgata gaagtggaac atcttcagaa acattttgtg aagattgtta aggaaccggg
      721 cttgaagggg gctttgcgct cctttattca tcctgaaaag cagacctttg aagcggtcaa
      781 ggatttgacc tttgaggttc caaaagggca gattttagga tttatcgggg caaatggtgc
      841 tgggaagtcg acaaccatta aaatgctgac aggaattttg aaaccaacat ctggtttttg
      901 tcggattaac ggcaagattc cccaggacaa tcggcaagat tatgtcaaag atattggcgt
      961 agtctttgga caacgcaccc agctatggtg ggatttggct ctgcaagaga cctacactgt
     1021 cttaaaagag atttatgatg tgccaaactc gctctttcat aagcgtatgg actttttgaa
     1081 tgaagtcttg gatttgaagg actttatcaa ggatcccgtg cggactcttt cactgggaca
     1141 acggatgcga gcggatattg cggcctcctt gctccacaat cccaaggttc tttttttaga
     1201 tgagccgacc attggtttgg acgtttcggt taaggataat attcgtcggg caattactca
     1261 gatcaatcaa gaggaagaaa ctaccattct tttgaccact cacgatttga gtgatattga
     1321 gcaactttgt gatcggattt tcatgattga caaggggcaa aagatttttg atggaacggt
     1381 gagccaactc aaggagacct ttggtaagat gaagactctc tcttttgaac tgctaccagg
     1441 tcaaagtcat ctcgtctctc actatgaagg tctgtctgat atgaccattg atagacaagg
     1501 aaacagcctc aacattgaat ttgatagttc tcgctaccag tcagctgaca ttatcaagca
     1561 aaccctatct gattttgaaa tccgcgattt gaagatggtg gatacggata ttgaggatat
     1621 tatccgtcgc ttctaccgaa aggagctcta ggatgatcaa attgtggaga cgttataaac
     1681 cctttatcaa tgcaggggtt caggagttga ttacttaccg agtcaacttt attctctatc
     1741 ggattggcga tgtcatgggg gcttttgtgg ccttttatct ctggaaggct gtctttgatt
     1801 cttcgcaaga gtctttgatt cagggcttca gtatggcgga tatcaccctc tacatcatca
     1861 tgagttttgt gaccaatctt ctgactagat ccgattcgtc ctttatgatt ggggaggagg
     1921 tcaaggatgg ctccattatc atgcgtttgt tgcgaccagt gcattttgcg gcctcctatc
     1981 ttttcaccga gcttggttcc aagtggttga tttttatcag cgttggcctt ccatttttaa
     2041 gtgtcattgt cttgatgaaa atcatatcgg gtcaaggtat tgtagaggtg ctaggattaa
     2101 ctgtccttta tctttttagc ttaacgctcg cctatctgat taactttttc tttaatattt
     2161 gctttggatt ttcagccttt gtgtttaaaa atctttgggg ttccaaccta cttaagactt
     2221 ccatagtggc ttttatgtcg gggagtttga ttcccttggc attctttcca aaggttgttt
     2281 cagatattct ctcctttttg cctttttcgt ccttgattta tactccagtt atgatcattg
     2341 ttggaaaata cgatgccagt cagattcttc aggcactcct tttgcagttc ttctggctct
     2401 tagtgatggt gggattgtct cagttaattt ggaaacgggt ccagtccttt atcaccattc
     2461 aaggaggtta gtatgaaaaa atatcaacga atgcatctga tttttatcag acaatacatc
     2521 aaacaaatca tggaatataa ggtggatttt gtggttggtg tcttgggagt ctttctggct
     2581 caaggcttga atctcttgtt tctcaatgtc atctttcaac atattccatt cctagaaggc
     2641 tggacctttc aagagatagc tttcatttat ggattttcct tgattcccaa gggaatggac
     2701 catctctttt ttgacaatct ctgggcacta gggcaacgcc tagtccgaaa aggagagttt
     2761 gacaagtatc tgactcgtcc catcaatcct ctctttcaca tcctagttga aacctttcag
     2821 attgatgcct tgggtgaact cttagtcggt ggtattttat tgggaacaac agtgaccagc
     2881 attgtttgga ctcttccaaa attcctgctt ttcctagttt gtattccttt tgcgaccttg
     2941 atttatactt ctcttaaaat cgcaacagcc agtatcgcct tttggactaa gcagtcaggc
     3001 gccatgattt acatcttcta tatgttcaat gactttgcta agtatccgat ttctatttac
     3061 aattctcttc ttcgttggtt gattagcttt atcgtgcctt tcgcctttac agcctactat
     3121 ccagctagct atttcttaca ggaaaaggat gtgttcttta acgtaggagg tttgatgttg
     3181 atttctctgg ttttctttgt tatttccctt aaactttggg ataagggctt agattcctac
     3241 gaaagtgcgg gttcgtaaaa gctaaagtaa gactaaaatc aagaaaggaa cttatgatgt
     3301 ttgtaattga agaagtcaag gatgaaaatc aaaaaaggca gttgtcgttg aggttttgaa
     3361 ggatttgcca gaatggtttg gaatcccaga aagcacacaa gcctatatag aaggaaccac
     3421 gacactgcaa gtttggaccg cctatcagga gagtgatttg actggatttg taaggttatc
     3481 ctattcgagt gaagattgtg cagagattga ttgtctcggc gtaaaaaagc ttatcaaggt
     3541 agaaaaattg ggagccaatt gcttgctact ttagagagtg aagctcgtaa aaaagttggt
     3601 tatctgcagg tcaaaacagt ggcagaaggt tctaataaag attatgatcg aacaaatgac
     3661 ttttatcgag gtcttggctt taaaaagtta gagatttttt ctcagctatg gaatccgtaa
     3721 aatccttgtc agattttgat taaaaagctt gaataatatt acttgacatc tattctcaga
     3781 gtgccatact gtaagtgtaa tcgccgattt agctcagttg gtagagcaag gcactcgtaa
     3841 agcctaggtt ataggtagat aaacgactga ggagttgaaa aaatagatag gtagaagata
     3901 accgttaagc cttattctta gcggttattt atattgttta atagcgctga tatttcatca
     3961 attatgcctg ttttcgtgtt tctggtagtt gttcaagttt attgctacta tttttgatgg
     4021 tatgaatgtg cttataatgt atcccggtta acgaaagttt tggacttata ctcatagaaa
     4081 atctcttcaa accacgtcaa cgtcgccttg ccgtgcgtat ggttactgac ttcgtcagtt
     4141 ctatccacaa cctcaaaaca gtgttttgag ctgactacgt cagttccatc tacaacctca
     4201 aaacagtgtt ttgag
//
LOCUS       DAVMGZ010000131         4127 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000131 DAVMGZ010000000
VERSION     DAVMGZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.132"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            17..>4127
                     /locus_tag="VYQ93_001790"
     CDS             17..>4127
                     /locus_tag="VYQ93_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV3336433.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQ"
BASE COUNT         1603 a          606 c          823 g         1095 t
ORIGIN      
        1 tatgtaagga attaaaatga aaaaaagtac agtattgtca ttaaccacag ctgcagttat
       61 tttagcagcc tatgccccta atgaggtagt cttagcagac acatctagct ctgaagatgc
      121 tttaaacatc tctgataaag aaaaagtagt attagataag gaaacagaaa ataaagagaa
      181 acatgaagat attcataatg ctatagaaac ttcaaaggat actgaagaaa agaaaacaac
      241 agttattgag gaaaaagaag ttgttagtaa aaatcctgtg atagacacta aaactagcaa
      301 tgaagaagca agaatcaaag aagattccaa tcaatcccaa ggagataatg cacactcgtc
      361 tgcaaataaa ggcacagaaa atccgaaaaa agaagataga cttgtctata ttgctgaatt
      421 taaagataaa gaatctggag aaaaagcaat caagcaacta tccaatctta agaatacaaa
      481 agttttatat acttatgata gaatttttaa cggtagtgcc atagaaacaa ctccagataa
      541 cttggacaaa attaaacaaa tagaaggtat tacatcggtt gaaagggcac aaaaagtcca
      601 acccatgatg aatcatgcca gaaaggaaat tggagttgag gaagctattg attacctaaa
      661 gtctatcaat gctccgtttg ggaaaaattt tgatggtaga ggtatggtca tttcaaatat
      721 cgatactgga acagattata gacataaggc tatgagaatc gatgatgatg ccaaagcctc
      781 aatgagattt aaaaaagaag acttaaaagg aactgataaa aattattggt tgagtgataa
      841 aatccctcat gcgttcaatt attataatgg tggcaaaatc actgtagaaa aatatgatga
      901 tggaagggat tattttgacc cacatgggat gcatattgca gggattcttg ctggaaatga
      961 tactgaacaa gatatcaaaa actttaacgg catagatgga attgcaccta atgcacaaat
     1021 tttctcttac aaaatgtatt ctgacgcagg atctgggttt gcgggtgatg aaacaatgtt
     1081 tcatgctatt gaagattcta tcaaacacaa cgttgatgtt gtttcggtat catctggttt
     1141 tacaggaaca ggtcttgtag gtgagaaata ttggcaagct attcgggcat taagaaaagc
     1201 aggcattcca atggttgtcg ctacgggtaa ctatgcgact tctgcttcaa gttcttcatg
     1261 ggatttagta gcaaataatc atctgaaaat gaccgacact ggaaatgtaa cacgaactgc
     1321 agcacatgag gatgcgatag cggtcgcttc tgctaaaaat caaacagttg agtttgataa
     1381 agttaacata ggtggagaaa gttttaaata cagaaatata ggggcctttt tcgataagaa
     1441 taaaatcaca acaaatgaag atggaacaaa agctcctagt aaattaaaat ttgtatatat
     1501 aggcaagggg caagaccaag atttgatagg tttggatctt aggggcaaaa ttgcagtaat
     1561 ggatagaatt tatacaaagg atttaaaaaa tgcttttaaa aaagctatgg ataagggtgc
     1621 acgcgccatt atggttgtaa atactgtaaa ttactacaat agagataatt ggacagagct
     1681 tccagctatg ggatatgaag cggatgaagg tactaaaagt caagtgtttt caatttcagg
     1741 agatgatggt gtaaagctat ggaacatgat taatcctaat aaaaaaactg aagtcaaaag
     1801 aaataataaa gaagatttta aagataaatt ggagcaatac tatccaattg atatggaaag
     1861 ttttaattcc aacaaaccga atgtaggtga cgaaaaagag attgacttta agtttgcacc
     1921 tgacacagac aaagaactct ataaagaaga tatcatcgtt ccagcaggat ctacatcttg
     1981 ggggccaaga atagatttac ttttaaaacc cgatgtttca gcacctggta aaaatattaa
     2041 atccacgctt aatgttatta atggcaaatc aacttatggc tatatgtcag gaactagtat
     2101 ggcgactcca atcgtggcag cttctactgt tttgattaga ccgaaattaa aggaaatgct
     2161 tgaaagacct gtattgaaaa atcttaaggg agatgacaaa atagatctta caagtcttac
     2221 aaaaattgcc ctacaaaata ctgcgcgacc tatgatggat gcaacttctt ggaaagaaaa
     2281 aagtcaatac tttgcatcac ctagacaaca gggagcaggc ctaattaatg tggccaatgc
     2341 tttgagaaat gaagttgtag caactttcaa aaacacggat tctaaaggtt tggtaaactc
     2401 atatggttcc atttctctta aagaaataaa aggtgataaa aaatacttta caatcaagct
     2461 tcacaataca tcaaacagac ctttgacttt taaagtttca gcatcagcga taactacaga
     2521 ttctctaact gacagattaa aacttgatga aacatataaa gatgaaaaat ctccagatgg
     2581 taagcaaatt gttccagaaa ttcacccaga aaaagtcaaa ggagcaaata tcacatttga
     2641 gcatgatact ttcactatag gcgcaaattc tagctttgat ttgaatgcgg ttataaatgt
     2701 tggagaggcc aaaaacaaaa ataaatttgt agaatcattt attcattttg agtcagtgga
     2761 agaaatggaa gctctaaact ccaacgggaa gaaaataaac ttccaacctt ctttgtcgat
     2821 gcctctaatg ggatttgctg ggaattggaa ccacgaacca atccttgata aatgggcttg
     2881 ggaagaaggg tcaagatcaa aaacactggg aggttatgat gatgatggta aaccgaaaat
     2941 tccaggaacc ttaaataagg gaattggtgg agaacatggt atagataaat ttaatccagc
     3001 aggagttata caaaatagaa aagataaaaa tacaacatcc ctggatcaaa atccagaatt
     3061 atttgctttc aataaccaag ggatcaacgc tccatcatca agtggttcta agattgctaa
     3121 catttatcct ttagattcaa atggaaatcc tcaagatgct caacttgaaa gaggattaac
     3181 accttctcca cttgtattaa gaagtgcaga agaaggattg atttcaatag taaatacaaa
     3241 taaagaggga gaaaatcaaa gagacttaaa agtcatttcg agagaacact ttattagagg
     3301 aattttaaat tctaaaagca atgatgcaaa gggaatcaaa tcatctaaac taaaagtttg
     3361 gggtgacttg aagtgggatg gactcatcta taatcctaga ggtagagaag aaaatgcacc
     3421 agaaagtaag gataatcaag atcctgctac taagataaga ggtcaatttg aaccgattgc
     3481 agaaggtcaa tatttctata aatttaaata tagattaact aaagattacc catggcaggt
     3541 ttcctatatt cctgtaaaaa ttgataacac cgcccctaag attgtttcgg ttgatttttc
     3601 aaatcctgaa aaaattaagt tgattacaaa ggatacttat cataaggtaa aagatcagta
     3661 taagaatgaa acgctattcg cgagagatca aaaagaacat cctgaaaaat ttgacgagat
     3721 tgcgaacgaa gtttggtatg ctggcgccgc tcttgttaat gaagatggag aggttgaaaa
     3781 aaatcttgaa gtaacttacg caggtgaggg tcaaggaaga aatagaaaac ttgataaaga
     3841 cggaaatacc atttatgaaa ttaaaggtgc gggagattta aggggaaaaa tcattgaagt
     3901 cattgcatta gatggttcta gcaatttcac aaagattcat agaattaaat ttgctaatca
     3961 ggctgatgaa aaggggatga tttcctatta tctagtagat cctgatcaag attcatctaa
     4021 atatcaaaag cttggcgaga ttgcagaatc taaatttaaa aatttaggaa atggaaaaga
     4081 gggtagtcta aaaaaagata caactggggt agaacatcat catcaag
//
LOCUS       DAVMGZ010000132         4093 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000132 DAVMGZ010000000
VERSION     DAVMGZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.133"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            14..823
                     /locus_tag="VYQ93_001791"
     CDS             14..823
                     /locus_tag="VYQ93_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV3336434.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            828..1529
                     /locus_tag="VYQ93_001792"
     CDS             828..1529
                     /locus_tag="VYQ93_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV3336435.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1554..2471
                     /locus_tag="VYQ93_001793"
     CDS             1554..2471
                     /locus_tag="VYQ93_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEV3336436.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2499..4040
                     /locus_tag="VYQ93_001794"
     CDS             2499..4040
                     /locus_tag="VYQ93_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV3336437.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
BASE COUNT         1330 a          630 c          796 g         1337 t
ORIGIN      
        1 ctaaggagct catatgttac tgcaaaaaga actaattcca atgatagaag ctaacttacc
       61 aaatatggca tatgctgaaa aagacattgc taaattcttc ttaaaacagc aacctctgaa
      121 tgattattca tctaaagcat tgtgcgaata ccttaatgta tccaaagcaa cattgactcg
      181 atttgcgaaa aaatgtggtt ttaaaggttt tagacaattc attttcaaat accaagagat
      241 gattcatgag aaagaaaagt tggcattata tacagaggca acagaaaaag ttttatccga
      301 ctatgaggaa atgttgagaa aaacttacac ggttcttgat gaagttcaac ttgagcgtat
      361 tgctgagatg atagaaactg ctgagcgtgt atatctctac ggtaaaggaa gttctgttct
      421 tgctttacaa gaaatgaaga tgagatttat gcgtctcgga gtgattggtg aagtattatc
      481 agacgaggat atgattttgt ggagtagctt actacttaat gaaaattgcc ttgtcattgg
      541 agcatccatt tcaggtcaaa ctgatattgt actagaaggt ctacaaaaag ctgcagataa
      601 aggcgctaaa acagttttaa tgactacaag aaaatttgac gaagaagatt gtttctttga
      661 tgaactattg ttattagctt cgaccgatca tctctcgtat ggcaatcgca tatcacctca
      721 gtttccaata cttttaatta cagactgctt attctctaat tatctggaaa gtccagagag
      781 acaatattat tacaatcaaa ctattatcca taaggaggaa taataaaatg agccaaattt
      841 ggactaaaga aaaatttata agccaagttc aaggtggagt cattgtttct tgtcaagctt
      901 tacctggtga agccctttat aatgaagaat ttagcttgat gccttttatg gctaaagcag
      961 ctttagaggc aggagcagtg ggcattcgcg caaattctgt gcgtgatatt aaagcaattc
     1021 agaaagtagt agatttacca ataattggaa ttatcaaaag ggattatcca cctcaagaac
     1081 catatattac tgctacgatg aaagaagtag atgaacttgt agaatgcgga acaacagtca
     1141 ttgcatttga tgcaacttta agaccaagat atgatggctt agttgtcagt gaatttatca
     1201 aaaaaataaa agaaaaatat ccgaatcaat tgctgatggc ggatgtaagt aatttagatg
     1261 aaggtctcta tgcatttaaa tcaggcgttg attttgttgg tacaacatta tcaggttaca
     1321 caagtacaag tgtacaatca gatgagcctg attttgaact aatgaaaaaa ttggctgatt
     1381 ttaatattcc ggtaattgcc gaaggaaaaa ttcattatcc agaacaatta aaaaaagctt
     1441 atagtttagg tgttaccagt gtagtcattg gtggagcgat tacacgtcca aaagaaattg
     1501 ctcagcgatt tattaatgtc atcaaataaa aaacaatata aaggagaaat ctgatgaaag
     1561 atttaactaa atacaaaggc gttatccctg cattttatgc ttgctatgat gaaaatggtg
     1621 aaattagcca agatcgtgta aaatctctgg tacaatattt cattgacaaa ggtgtaaaag
     1681 gtatctatgt aaatggttct tcaggtgaat gtatttacca aagtgtagaa gatcgtaaac
     1741 aaattattga agctgttatg gaagttgcta aaggtaaatt aacagttatc aaccatattg
     1801 catgtaataa cacgaaagat agtatcgaat tggcaaaaca ttcagaaagt gttggagtcg
     1861 atgctattgc agctatccca cctatttatt tcaaattgcc agagtattca atcgcagcat
     1921 attggaatgc aatgagtgaa gctgcgtcaa atacagattt tattatctat aatattccac
     1981 aattggcagg ggttgcgttg actggtagtt tgtatgcaac aatgcgtcaa aatcctcgtg
     2041 tgattggagt taaaaattct tctatgcctg tacaagatat tcaaatgttt gtagctgcag
     2101 gtggagaaga ttacattgta ttcaatggtc cagatgaaca atatcttggt ggtcgcttga
     2161 tgggagcaga agctggtatt ggtggtactt atggcgttat gccagatttg ttcttgaaat
     2221 tggaaagttt gattcaagaa cgagatttag atacagctaa aaaacttcaa tatgctatca
     2281 atgaagttat ctataagatg atatcaggta aggcaaatat gtatgctgta gcaaaagaag
     2341 ttttgcgtct aaatgaaaaa cttgatttag gttctgttcg tcaaccttta gaagcattgg
     2401 cagaaggtga cttggaagtt gcaaaacaag cagcagaact tattcaacaa gcacgaaaag
     2461 aatttttata ataaataaaa tcaatatgga gatatgttat gggcacaaca ggatttacaa
     2521 taattgactt aattatcttg attgtttatt tacttgcggt gttggttgca ggtatctatt
     2581 tctctaaaaa agagatgaaa ggaaaagagt tctttaaagg agatggttcg gttccttggt
     2641 atgttacttc ggtatccatt tttgccacaa tgctcagtcc gatttccttc ttgggactcg
     2701 ctggtagctc ttatgcaggt agctggattt tatggtttgc tcaattaggg atggtagtag
     2761 ctattccact gacaattcgt tttatcttac ctatctttgc acggatagac atcgatacgg
     2821 catatgatta cttggataaa cgttttaatt ctaaagcact tcgtattatt tcagcactct
     2881 tgtttattat ttatcaattg ggacgtatgt ctatcattat gtacctccca tcagctggtt
     2941 tatcagtatt gacaggaatt gacatcaata ttttgattat tttgatgggt gtagttgcaa
     3001 ttgtttattc ttatactggt ggtctaaaat ccgtattatg gacagacttt attcaaggtg
     3061 tgattctgat tagtggtgtc gttttagctt tatttgtact gattgctaat attaaaggtg
     3121 gctttggtgc agtagcagaa acattagcaa acgggaaatt ccttgctgca aatgaaaaac
     3181 ttttcgatcc taacttgctt tcaaactcca tctttttaat tgtgatgggt tcaggcttta
     3241 caatcttgtc ttcctatgct tcatctcaag atttggttca acgttttact acaacacaaa
     3301 atattaagaa acttaataag atgttgttca caaacggtgt tttgtcactt gcaactgcaa
     3361 cagtctttta cttgattggt acaggcttgt acgtattcta tcaagtacaa aatgcagata
     3421 gtgcagctag caatatccct caagaccaaa tctttatgta ctttattgca taccagttac
     3481 cagtaggtat cacaggtttg atcttggcag cgatttatgc agcatctcaa tcaactattt
     3541 caacaggttt gaactctgtt gcaacttcat ggacattgga tattcaagat gtcatttcta
     3601 aaaatatgtc agacaatcgt cgtacgaaaa ttgcacaatt cgtatctcta gcagtaggtt
     3661 tattctcaat tggtgtttcc attgtcatgg ctcactcaga tattaaatct gcatacgaat
     3721 ggttcaatag tttcatggga cttgtacttg gtctacttgg tggtgtattt attcttggat
     3781 ttgtttctaa aaaagcaaat aaacaaggtg cttatgcagc gctgattgta tcaaccatcg
     3841 tcatggtatt tattaaatac ttccttcctc caacagctgt tagctactgg gcatattcat
     3901 tgatttcaat ctctgtatca gtagtttcag gttatattgt atctgttctt actggaaata
     3961 aagtatctgc acctaaatat acaacgattc atgatattac agaaattaaa gcggattcaa
     4021 gttgggaagt tcgtcactaa tagaaaagag aaaataatat gattatttct gaacaatctg
     4081 attttaaacg ata
//
LOCUS       DAVMGZ010000133         4076 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000133 DAVMGZ010000000
VERSION     DAVMGZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.134"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            32..847
                     /locus_tag="VYQ93_001795"
     CDS             32..847
                     /locus_tag="VYQ93_001795"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV3336438.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            866..1507
                     /locus_tag="VYQ93_001796"
     CDS             866..1507
                     /locus_tag="VYQ93_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV3336439.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            1538..2539
                     /locus_tag="VYQ93_001797"
     CDS             1538..2539
                     /locus_tag="VYQ93_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV3336440.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            2549..3178
                     /locus_tag="VYQ93_001798"
     CDS             2549..3178
                     /locus_tag="VYQ93_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV3336441.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(3557..3942)
                     /gene="tnpA"
                     /locus_tag="VYQ93_001799"
                     /pseudo
     CDS             complement(3557..3942)
                     /gene="tnpA"
                     /locus_tag="VYQ93_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         1204 a          887 c          812 g         1173 t
ORIGIN      
        1 aagtcttaac aaatttgaaa ggaagtatca aatgacaaat acatcattct caattgagca
       61 gttttcttta aaaggaaaaa ttgctctcat caccggcgct tcttatggaa ttggatttgc
      121 tattgccaaa tcctacgctg aagccggcgc tactattgtc tttaacgata tcaatcaaga
      181 tctggtcaat aaagggattg aagcttatcg tgaagttggc atccaagccc atggatatgt
      241 ctgtgacgtg acagacgagg acggtatcca agccatggtc aagcaaatcg aacaagaggt
      301 tggtgtcatt gacatcctcg ttaataacgc tggtattatc cgccgagttc caatgtgcga
      361 aatgagcgcc gctgatttcc gtaaggtcat cgatattgac ttaaacgcac catttatcgt
      421 ttcaaaggca gttattcctt ctatgataaa gaaagggcat ggaaagatta tcaatatttg
      481 ttcgatgatg agcgaactgg gacgtgaaac agttagcgct tatgctgctg ctaaaggggg
      541 cttgaaaatg ttgacccgca acattgcgtc tgaatacggt ggagccaata tccaatgtaa
      601 cggaattgga ccaggttata ttgccactcc tcaaacagca cctcttcgtg agttgcaaga
      661 agatggttct cgccacccat ttgaccagtt catcattgca aaaacacctg ctgcacgttg
      721 gggaaatcct gaagatttga tgggccctgc tgtctttctc gctagtgatg ccagcaattt
      781 tgtcaatggc cacatcctat atgtagatgg cggtatctta gcctacatcg gaaaacaacc
      841 tgagtaaaaa tagaaagaag atcttatgaa aatcgcatta atcaatgaaa atagtcaagc
      901 tagcaagaat cacattattt acgatagtct aaaagaagcg acagataaaa aagactacca
      961 attatttaac tatggtatgc gtggagaaga aggagaaagt caattaactt atgtgcagaa
     1021 cggactaatg gctgccatcc ttttaaatac aaaggcagtt gactttgttg ttaccggctg
     1081 tggtacgggt gtaggggcta tgcttgcttt aaacagcttc cctggtgttg tctgtggtct
     1141 agcagtggac ccaactgacg cttaccttta ttctcaaatc aatggtggta acgccttgtc
     1201 tatcccttat gccaaaggat ttggctgggg ggcagaactg accctcaaat tgatgtttga
     1261 acgcttattt gctgaagaaa tgggcggtgg ctacccaaga gaacgtgtaa tccctgaaca
     1321 acgtaacgct cgtatcttaa acgaggtgaa acaaatcacc cacaatgatt tgatgaccat
     1381 ccttaaaaca atcgaccaag acttcctcaa agacaccatc tctggcaaat acttccaaga
     1441 atacttcttt gaaaactgcc aagatgatga agtcgctgct tatttgaaag aagtattagc
     1501 caagtaaagc tattctaaac cagaaaggaa ctaatggatg acgaaaatat tactgtttgg
     1561 cgaaccatta attcgaatct caccattaga tgccaccagt atcggcgatc atgttgccag
     1621 ttcgacttat tttggcggat cagaaattaa catcgcttgt aatttgcaag ccctgggtat
     1681 ctcaacgaaa gtctttaccg cactccctgc caacgagatt ggagatcgtt ttctcacatt
     1741 cttgaaacag caccaaatcg ataccagttc aatctgtcgg cttggcgatc gaatcggcct
     1801 ctactatttg gagaacggct ttggttgtcg tcaaagtgaa gttttctacg atcgtaagca
     1861 tacgagtatc agccagattc ggccaaacat gctagatatg gattctctct ttcaggggat
     1921 tagccatttt cattttagtg gaatcaccat agctatcggt caagaggtcc gtgcgatcct
     1981 tctcctactc ttggaagaag ccaagcgccg aggaattgtc gtttcaatgg atctcaatct
     2041 gagaacaaag atgatttcag tcctagaagc caagtatgaa ttttctaagt ttgcacgttt
     2101 tactgactat tgcttcggta ttgatcctct catgattgat gaccaaaatc tagagatgtt
     2161 tccaagagac agtgctagcc tagaagaggt ggaaaatcgc atgcgacttt taaaagaagc
     2221 ctatggtttc aaggccattt tccataccct ccgatctagt gatgagcaag acaaaaatgt
     2281 ctatcaagcc tatgctctgg aagaacgatt tgaagagtct gtccaactaa aaactgcagt
     2341 ctatcaacga attggtagcg gggatgcctt tatatctggt gccctttacc aactactcca
     2401 tcattcctcc ctaaaaacta ccattgactt tgcagttgcg agcgcaactc tcaaatgcac
     2461 tcttccagga gaccatctct ccacttccgc aactagtatt gaaaatttac tggcaaatgc
     2521 acaagatatc attcgttagg agaattacat gaccaaatca gatacgatta ttgaactaaa
     2581 aaaacagaaa attgtcgctg tcattcgagg aaatacaaag gaagaaggac tacaagcctc
     2641 gattgcttgt atcaagggcg gtatcaaagc tattgaaatc gcctatacca atcagtatgc
     2701 aggacaaatc atcaaggaac ttgtagactt gtatcaggac gatcagagtg tttgtatcgg
     2761 tgcaggtact gtgcttgatg ccgtaactgc tagagatgcc attctagctg gagcaaatta
     2821 cgttgtttct ccatctttcc atgctgaaac tgcgaaaatg tgcaatctct acagcacacc
     2881 gtacattcca ggctgtatta ccctcacaga gatcacgact gcacttgaag ccggtagtga
     2941 aatcatcaaa ctcttcccag gtagtactct cagtccagca tatatctctg cagtcaaggc
     3001 accgatccca caagtttccg taatggtaac cggaggagtc ggcctaaaca acatccctca
     3061 atggttcgct gctggtgcag atgccgttgg aattggtggc gaactcaata aactcgcttc
     3121 ccaaggcaac tttgaccgca tcagcgagat tgcccaacag tatattacac tcagataaaa
     3181 tcataactac ccgtctaacg ggtggtttat cccagagcta taagcccaaa tcatcagcca
     3241 gcgcctaaag acgctggctt tcacgttgtt caagccttat tgctcttgac tcgtcacttg
     3301 cctctttaag agactttggt attacttacc actatcccta aagggatcct catattcttt
     3361 tacactcaat ttatctagtg ctatagtaga ttgaaactgg aatagtatac ctctacttct
     3421 aaaacattgt taaaaatcga tttgactgtc ctgatcgatt tcgtcctgtt cttatttcat
     3481 tttactatat atcatacttt actagttctc aaattttcat actcgtgaag aaatcatcca
     3541 ctcgataatt tctttaatct tgactatatt tcttaattgt ggcttcatta agccctactg
     3601 gacttacata ataaccttcc gcccagaaat tccgattccc aaacttgtac ttgagattgg
     3661 cgtgtttgtc aaacatcatg agtgcacttt tgccttttaa ataccccata aaacttgaaa
     3721 cacttagcct cgacggaata ctgactaaca tgtgtacatg gtctggcatt aagtgaccct
     3781 cgatcatttc aacaccttta taactacaca agcgatgaaa tatttcgcct aaactacttc
     3841 tatattgatt atagatgact tttcgtctat acttaggggt gaacacaata tgatagaaca
     3901 catccacttt gtgtatgata aactatgagt cttttgtgcc atattttttc tcctttcgct
     3961 ttacaattgg attgaatacc tttattgtat cgcgtttgga gtttttttgg tataaccttc
     4021 gacgcgcacc tcacggagcg agacggactt atatagtgga gtgaaatagg atacga
//
LOCUS       DAVMGZ010000134         4040 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000134 DAVMGZ010000000
VERSION     DAVMGZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.135"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            18..971
                     /locus_tag="VYQ93_001800"
     CDS             18..971
                     /locus_tag="VYQ93_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3336442.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            1048..3036
                     /gene="uvrB"
                     /locus_tag="VYQ93_001801"
     CDS             1048..3036
                     /gene="uvrB"
                     /locus_tag="VYQ93_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV3336443.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVQALD"
     gene            3046..3609
                     /locus_tag="VYQ93_001802"
     CDS             3046..3609
                     /locus_tag="VYQ93_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3336444.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNIGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            3575..>4040
                     /locus_tag="VYQ93_001803"
     CDS             3575..>4040
                     /locus_tag="VYQ93_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3336445.1"
                     /translation="MTVIIKSMETPEEIEDKSLVHWQTWREAYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEK"
BASE COUNT         1183 a          715 c          938 g         1204 t
ORIGIN      
        1 gaaaagagga cggggatatg aagaacaaaa gaatatttaa agacttccaa gcttcaaaaa
       61 tgagtttaaa catttacaca agccccttgt tagcctttgt ttttgtcttc ataggagagt
      121 ttgtggcttt tactttgtat ggtattggct tgttagctct catcggactt gctagaaatt
      181 ttggagaggc tggtcaaaat cttgcaagct acttgcagac cttgcatcag agcttgacgg
      241 ataaaacaag tgactttcgt ttaattttag gattactggc ctttggtttt attcttaaca
      301 ctgtgttcag atggacaaga aaagttgaga aaagacctat tcgaaccttg ggattttata
      361 gagagaattt cctcagcaat cttctgaaag gatttagtct aggcctggca ctttttcttc
      421 tgaccttgtt aggtttagtg gtcttaggtc aatatcgttt ggaatccatt cacttgaatc
      481 cttattctct tgcctttgtc gtctttacta tcccattttg gattttacag gggacagcag
      541 aagaagtggt ggcccgtgct tggctacttc ctcaattggc ctcaagaacc aatctaaaac
      601 tagctattct tatatctagc ctgttcttta ccctgcttca tatgggcaat tctggtctca
      661 cccctctatc tctagtaaat ctctttttat tcggagttgc catggctctt taccttctca
      721 aaactgatac agtttggggt gttgcaggta ttcatggtgc ttggaatttt gctcagggta
      781 atctctttgg gattttagtt agtggtcaac cgtcaggaac gtctctgatg acctttttac
      841 cacaaggcaa tcaagattgg ctatcaggtg gttcttttgg catagaaggt tccattatga
      901 caagtctggt attactactg ctgattgtct atcttgctaa taaattaaag aaagaaaatg
      961 aaaggatgtg acttcggtcc gtccttttct tcgtgaaaat actataagta tgctaaaata
     1021 ggaatagcac atggagagag gattcttatg atcaatcaca ttacagataa tcaatttaaa
     1081 ctagtatcaa aatatcaacc atcaggagat caaccccaag ctatcgagca gttggtggat
     1141 aacattgagg ggggagaaaa agctcagatt ctgatggggg cgactggaac agggaagacc
     1201 tatactatga gtcaggtcat ttctaaagtc aataaaccaa ctctggttat tgcccacaat
     1261 aaaactctgg ctggtcagct ctatggggag tttaaggaat ttttccctga aaatgcagtt
     1321 gagtatttcg tatcctacta tgattattac cagccagagg cctatgtccc ttctagcgat
     1381 acctatattg agaaggatag ttctgtcaat gacgagattg acaagcttcg ccactcagct
     1441 acctcagccc ttttggagcg taatgatgtt attgtcgtgg cctcagtctc ttgtatctat
     1501 ggtttgggtt cgcccaagga atacgctgat agtgtcgtta gtctccgtcc tggtctagag
     1561 atttctcgtg ataaactctt gaatgacttg gtcgatattc agtttgaacg taatgatatt
     1621 gatttccaac gcggaagatt tcgcgttcgt ggggatgtgg tagagatttt cccagcttcc
     1681 cgagatgaac atgcctttcg agtagaattt tttggagacg aaattgaccg tattcgtgaa
     1741 gttgaggctc tgacaggtca ggtgttggga gaagtggatc atttagcgat tttcccagcg
     1801 acacactttg tgaccaatga cgaccacatg gaagttgcca ttgcaaagat tcaggccgag
     1861 ttggaagaac aattagctgt ctttgaaaag gaaggtaaac tgcttgaagc ccagcgtttg
     1921 aaacagcgga cagagtatga tatcgaaatg ttacgtgaga tgggctatac caatggggtt
     1981 gaaaattatt ctcgccacat ggatggacgg agcgaaggag agcctcctta tacgcttctc
     2041 gacttcttcc cagatgattt cttgattatg attgacgaga gtcatatgac catagggcaa
     2101 atcaagggca tgtacaatgg agaccgttcg cgtaaagaaa tgctggttaa ttatggtttc
     2161 cgtttgccgt ctgctttgga caatcgtcct ctccgtcggg aggagtttga gagtcacgtt
     2221 catcagattg tttacgtttc agcgacacct ggtgactatg aaaatgaaca gaccgagaca
     2281 gtgattgagc aaatcattcg tccaacggga ctcttggatc cagaggtgga agtccgtccg
     2341 actatgggac agattgatga cctcttgggt gaaatcaatg cccgcgttga aaaaaatgag
     2401 cgtaccttta tcacaacttt gaccaagaaa atggcagagg atttgaccga ctacttcaag
     2461 gaaatgggta tcaaggtcaa gtacatgcac tcggatatca agaccttgga acggacggag
     2521 attatccgtg acctgcgctt gggtgtcttt gatgtcttgg tcggaattaa cctgctccgt
     2581 gaaggaattg acgttcctga agtgagcctc gtagctattc tcgatgctga caaggaaggt
     2641 ttccttcgca acgaacgtgg actcatccag actattggac gtgctgctcg taacagtgaa
     2701 ggccatgtca tcatgtatgc agacacggtt acccagtcta tgcaacgtgc tatcgatgaa
     2761 actgcccgcc gtcggaaaat tcagatggcc tataatgaag agcatggtat cgtaccacag
     2821 acaatcaaga aagaaatccg tgacctgatt gccgtgacca aggcagttgc taaggaagag
     2881 gacaaggaag tcgacatcaa tagcctcaac aaacaagagc gcaaagaact cgtcaagaaa
     2941 ctagaaaaac aaatgcaaga agccgtcgaa gtgcttgact ttgaactggc ggctcagatc
     3001 cgtgatatga tgttggaagt ccaggccttg gattagggga atagtatgat ttatttaaga
     3061 aagttaaaga gagaagattt gatgtcttta tgggaaatgg cttattcaca acttaatcca
     3121 gtttggaaac agtatgatgc tccctattat gatgattatc agtatttttc aaattttaaa
     3181 gaattcgaac tacaaaaatc agaatccatt ttaagcaact caaatcgcct tggtattttt
     3241 gttgatgata aactagttgg gactgtttcg cgttattggg tatgtaaaca aacaagatgg
     3301 atggaattgg gaattggtat ttatgataaa aaattctgga acactggtat tgggaaagtt
     3361 gctatgttgc agtggataga taggacgttt caggattact tggagttgga gcatctgggt
     3421 ttgacaactt ggtcagggaa tattggtatg atgaaacttg ctgaaaaatt aagaatgaaa
     3481 aaagaagctc atattccaaa agttcgttat tatcaaggta aatattttga tagtattaaa
     3541 tatggtattt tgagagaaga ctgggagaaa ataaatgacg gttattatca aatcaatgga
     3601 aactcctgaa gagatagaag ataaatccct tgttcactgg caaacgtgga gagaggctta
     3661 tgatgacctt ttgcctgcgg aatttcagga tacaatgaca ttagaaagat gtcgattctt
     3721 tagtcaaaag tatccagaaa atacattgat tgcgatggat ggcgtgaagg tggttggttt
     3781 tatcagttat ggcaattttc gtgatgagac tattcaagct ggtgaaatta ttgctttata
     3841 tgttttaaaa gactattatg gaaaaggtat cgcacaaaag ttaatgaaag cagctttgac
     3901 tgctcttaat catttttctg aaattttctt atgggtattg aaagataaca agcgcgccat
     3961 tgctttctat caaaaaatgg gttttacttt tgatggacaa gaaaaaatac ttgaacttgg
     4021 aaagcctata aaggaaaaac
//
LOCUS       DAVMGZ010000135         4035 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000135 DAVMGZ010000000
VERSION     DAVMGZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.136"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            27..1727
                     /locus_tag="VYQ93_001804"
     CDS             27..1727
                     /locus_tag="VYQ93_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV3336446.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1720..2196
                     /gene="ilvN"
                     /locus_tag="VYQ93_001805"
     CDS             1720..2196
                     /gene="ilvN"
                     /locus_tag="VYQ93_001805"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV3336447.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2262..3284
                     /gene="ilvC"
                     /locus_tag="VYQ93_001806"
     CDS             2262..3284
                     /gene="ilvC"
                     /locus_tag="VYQ93_001806"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV3336448.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3391..3627
                     /locus_tag="VYQ93_001807"
     CDS             3391..3627
                     /locus_tag="VYQ93_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336449.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            3865..>4035
                     /locus_tag="VYQ93_001808"
     CDS             3865..>4035
                     /locus_tag="VYQ93_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV3336450.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDL"
BASE COUNT         1252 a          699 c          948 g         1136 t
ORIGIN      
        1 tttttaagag gaaagaaagg ggaataatgg agaaaatcag tttagaatct cctaagacgg
       61 ggtcggacct agttttggaa acacttcgtg atttaggagt tgataccatc tttggttatc
      121 ctggtggtgc ggttttgcct ttttatgatg cgatatataa ttttaaaggc attcgccaca
      181 ttctagggcg ccatgagcaa ggttgtttgc atgaagctga aggctatgcc aaatcaactg
      241 gaaagttggg tgttgccgtc gtcactagtg gaccaggagc aacaaatgcc attacaggga
      301 ttgcggatgc tatgagcgat agcgttcccc ttttggtctt tacaggtcag gtggcgcgag
      361 cagggattgg gaaggatgcc tttcaggagg cagacatcgt aggaattacc atgccaatca
      421 ctaagtacaa ttaccaagtt cgtgagacag ctgatattcc gcgtatcatt acggaagctg
      481 tccatatcgc aactacaggc cgtccagggc cagttgtaat tgacctacca aaagacatat
      541 ctgctttaga aacagacttc atttattcac cagaagtgaa tttaccaagt tatcagccga
      601 ctcttgagcc gaatgatatg caaatcaaga aaatcttgaa gcaattgtcc aaggctaaaa
      661 agccagtctt gttagctggt ggtggaatta gttatgctga ggctgctacg gaactaaatg
      721 aatttgcaga acgctatcaa attccagtgg taaccagtct tttgggacaa ggaacgattg
      781 caacgagtca cccactcttt cttggaatgg gaggcatgca cgggtcattc gcagcaaata
      841 ttgccatgac ggaagcggac tttatgatta gtattggttc tcgtttcgat gaccgtttga
      901 cggggaatcc taagactttc gctaagaatg ctaaggttgc ccacattgat attgacccag
      961 ctgagattgg caagattatc agtgcagaca ttcctgtagt tggagatgct aagaaggcct
     1021 tgcaaatgtt gctagcagaa ccaacagttc acaacaatac tgaaaagtgg attgagaaag
     1081 tcactaaaga caagaatcgt gttcgttcgt atgacaagaa agagcgtgtg gttcaaccgc
     1141 aagcagttat tgaacgaatt ggtgaattga cgaatggaga tgccattgtg gtaacagacg
     1201 ttggtcaaca ccaaatgtgg acagctcagt attatcccta ccaaaatgaa cgtcagttag
     1261 tgacttcagg tggtttggga acaatgggct ttggaattcc agcagcaatc ggtgctaaaa
     1321 ttgctaaccc agataaggaa gtagtcttgt ttgttgggga tggtggtttc caaatgacca
     1381 accaggagtt ggctattttg aatatttaca aggtgccaat caaggtggtt atgctgaaca
     1441 atcattcact cggaatggtt cgccagtggc aggaatcctt ctatgaaggc agaacatcag
     1501 agtcggtctt tgataccctt cctgatttcc aattgatggc gcaggcttat ggtattaaaa
     1561 actataagtt tgacaatcct gagaccttgg ctcaagacct tgaagtcatc actgaggatg
     1621 ttcctatgct aattgaggta gatatttctc gtaaggaaca ggtgttacca atggtaccgg
     1681 ctggtaagag taatcatgag atgttggggg tgcagttcca tgcgtagaat gttaacagca
     1741 aaactacaaa atcgttcagg agtcctcaat cgctttacag gtgtcctatc tcgtcgtcag
     1801 gttaatattg aaagcatctc tgttggagca acagaagatc cgaatgtatc gcgtatcact
     1861 attattattg atgttgcttc tcatgatgaa gtggagcaaa tcatcaaaca gctcaatcgt
     1921 cagattgatg tgattcgcat tcgagatatt acagacaagc ctcatttgga gcgcgaggtg
     1981 attttggtta agatgtcagc gccagctgag aagagagctg agattttagc gattattcaa
     2041 cctttccgtg caacagtggt agacgtagcg ccaagctcga ttaccattca gatgacggga
     2101 aatgcagaaa agagcgaagc cctattgcga gtcattcgcc catacggtat tcgcaatatt
     2161 gctcgaacgg gtgcaactgg atttacccgc gattaaaaat ccaacttaaa tttgttaaac
     2221 cagcttaaaa ggcaataaat aatagaaaag agagaaaagc tatgacagtt caaatggaat
     2281 atgaaaaaga tgttaaagta gcagcacttg acggtaaaaa aatcgccgtt atcggttatg
     2341 gttcacaagg gcatgcgcat gctcaaaact tgcgtgattc aggtcgtgac gttattatcg
     2401 gtgtacgtcc aggtaaatct tttgataaag caaaagaaga tggatttgat acttacacag
     2461 tagcagaagc tactaagttg gctgatgtta tcatgatctt ggcgccagac gaaattcaac
     2521 aagaattgta cgaagcagaa atcgctccaa acttggaagc tggaaacgca gttggatttg
     2581 cccatggttt caacatccac tttgaattta tcaaagttcc tgcggatgta gatgtcttca
     2641 tgtgtgctcc taaaggacca ggacacttgg tacgtcgtac ttacgaagaa ggatttggtg
     2701 ttccagctct ttatgcagta taccaagatg caacaggaaa tgctaaaaac attgctatgg
     2761 actggtgtaa aggtgttgga gcggctcgtg taggtcttct tgaaacaact tacaaagaag
     2821 aaactgaaga agatttgttt ggtgaacaag ctgtactttg tggtggtttg actgccctta
     2881 tcgaagcagg tttcgaagtc ttgacagaag caggttacgc tccagaattg gcttactttg
     2941 aagttcttca cgaaatgaaa ttgatcgttg acttgatcta cgaaggtgga ttcaagaaaa
     3001 tgcgtcaatc tatttcaaac actgctgaat acggtgacta tgtatcaggt ccacgtgtaa
     3061 tcactgaaca agttaaagaa aatatgaagg ctgtcttggc agacatccaa aatggtaaat
     3121 ttgcaaatga ctttgtaaat gactataaag ctggacgtcc aaaattgact gcttaccgtg
     3181 aacaagcagc taaccttgaa attgaaaaag ttggtgcaga attgcgtaaa gcaatgccat
     3241 tcgttggtaa aaacgacgat gatgcattca aaatctataa ctaattagaa atatatagcg
     3301 ctggagatga ttttatgaaa aagattatga gaaaaattgc atcgttatta ttggttctag
     3361 ttgtataatg taattacacc gtcggtaata gtgctagcag accaaaataa agcagattgg
     3421 tcgtatgatg aaaatgctgt aattaacatt tatgatgatg ctaattttga agatggtagg
     3481 ttgcatatga cctttgaaca attcttcaaa ttggcacaaa tagctagaga agaaggtctt
     3541 gaaattcatt ctccgtttga gagagctggt gcgactaaat ctgctcgtta tatagcgaaa
     3601 tggattttga gaaataaaaa atattaacaa atatagttgg taaatcatta ggacctaaat
     3661 cagctgttag attcggagaa gctttatcct atattgaagg tcctcttcgc agaataaatg
     3721 agacgataga tggcggttta tatcaaatag agcaaattat tgcatctgga ttgaaagaat
     3781 cgggtttaaa tgactggact gcgaaaactt tagcttcagc tattcgtggg atattagatg
     3841 tacttattta ggggttgaaa tcatatgaat attaccaatt tgttttctat caagacagga
     3901 tgtgatgaaa ctgataggca actgcaaaaa ctattttttc agttggattt acaattggga
     3961 gaattgacag atcaactaag aaaattagat tctaattttg ttcctcgtag tcaatttgta
     4021 gacacgttgg atttg
//
LOCUS       DAVMGZ010000136         3872 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000136 DAVMGZ010000000
VERSION     DAVMGZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.137"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            33..485
                     /locus_tag="VYQ93_001809"
     CDS             33..485
                     /locus_tag="VYQ93_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336451.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDQPEDNLDNRYICRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            1304..1663
                     /locus_tag="VYQ93_001810"
     CDS             1304..1663
                     /locus_tag="VYQ93_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336452.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            1673..2038
                     /locus_tag="VYQ93_001811"
     CDS             1673..2038
                     /locus_tag="VYQ93_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV3336453.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYEQLVFELRRIGNNINQIARAINQSHLISQNQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSY"
     gene            2025..>3872
                     /locus_tag="VYQ93_001812"
     CDS             2025..>3872
                     /locus_tag="VYQ93_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV3336454.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSHAEMVEMYNVNFSNNDKLYESRNDRQEKHQQTIHAHYLIQSFSPEDNLTPEEINR
                     IGYEIMMELTGGRFKFIVATHTDKDHVHNHILINAIDRNSDKKLIWNYALERNLRMIS
                     DRISKVTGAKIIEKRYSYRDYKKYRESSHKFELKQRLYFLLQQSKSFDDFLEKAKQLH
                     VQIDFSQKHSRFLMTDRTMIKPIRGRQLSKRDLYDEDFFKTYFAKQEIESRLKFLLKS
                     VYSLEELHVKAKELNLTIELKQKNVMFTLEEDGKKISLSHKKISDKKLYDVQFFNRYF
                     EDREVRDIQALENLQEDFQTFREEQHKEKVSAEEIEEAFKKYKEKRDAIHEFEIELTD
                     NQIEKLVDDGVYIQVSFGIKQSGLIFIPNYQLDIFEEDNHKKYKVYIRETSSYFVYNK
                     ENMDNNCFIKGRTLIRQLSNDSQKLPYRRPTLKSLKEKISEINLMIELSNTNKQYQEI
                     KDELVLEIAEIDMQLEETQEKIATLNKMAEVLINLKSEDHETRKLAKYDFDQMNMTES
                     IMLDRLNTDILKLQQELGNEINKYEEIARRLDLFVKIINTNKFTVLKFHE"
BASE COUNT         1395 a          591 c          776 g         1110 t
ORIGIN      
        1 cgaaaaattt tttctaaaga aaatacacga ttttggagtt taatattaac tcgaaagaat
       61 cttcgcaaca acttgatatt ctctataaag aagttggtgt attaagtttg ggacaaaaag
      121 ttgtagccat gcttgatttt ttattagcat atagtgatta ttctaaagac ttcagaccat
      181 tgattattga tcagcctgaa gacaatctag acaatcgtta tatttgcagg catttagttc
      241 agcagtttag agatgtgaaa gctcaacgtc aaattatttt agcaacacat aatgctacaa
      301 ttgtaacaaa ttctatgaca gatcaagttg ttattatgga gtcagatgga gttaacggat
      361 ggattgaatc acagggatat gttagtgaaa aatatataaa aaatcatatc atcaatcaat
      421 tagagggagg aaaagattcc ttcaagcata aaatgtctat atatgagacg gctttatcag
      481 agtagagtca gaaaaagtag gttagaaatt tagcctactt ttttctttgt ccgacaggca
      541 tagtgtgcat ctgaggtcca agtcctctgt ggatatttgc tgcagatgaa accaatagcg
      601 actcctaagc ctgaatatcg tgaggtaggg gggataggaa ggaattagcg aaatcaaggt
      661 tctacaaaca gaatcgtgac ttgaagccat atatagcgga tgaggaactc taaaatccaa
      721 ataggtgtcg taacctatat acgtaaatta cgagagtaaa ctaggaaaga tgtacggctt
      781 attccgtgag cgtttaggac gtagtacaac gaatcatggg agtcagctga acacatagta
      841 ttgaagaaat ttctgtaatg gaaatggagc gaagaagtga acaattaaat gaatacctct
      901 ctaattaaat ttgtcaattc taattcctgg tatgaaaaga cagtgacctg aaaatgtaaa
      961 cgatgggagc tgattataaa tataggacgg tacatgcagt ggtgttagag attagtccct
     1021 acttgatttg tgataacttc cccaaatttc ttctgctata cttttctcaa cttttaaaaa
     1081 tccaactaag aattttacct gggggtttgg gggcgaagcc accaagttat cttatcgttg
     1141 gctgtcaaaa ctggaaggtt ttggttagct ggcgatatga ttttttgggt attgtaacca
     1201 caatgcttga gctcgcaaag accgaacaag gactttaaga aatttcccgt atgacacacg
     1261 gggtcggggt ttacccgaga aaggatgaaa gtgaggaaaa gagatgtctg aacagtaccg
     1321 tgacattcga aaagaggtaa atctaaccgc agatgagcta aaacagattg aaaaaatgat
     1381 ggaggttgat aattatcgtc acttttcacc attcgtcagg gataaaattt tgatgactga
     1441 tgacaaacag ttagctgcca aagaatggtt ttccctttgg cagtctcaaa agtttgaaca
     1501 gatcagtcga gatgtgcatt tggttttgat tatcgcaaga gagaatcacc aagtgactca
     1561 agaacacgtg tcaatcttat tgacctgcgt tcaagaattg attgcggaag tcaatcaagt
     1621 acagtcactc agtcgtgggt ttcgtgaaaa atacatgaga taggaggcta ctatggttta
     1681 tcgttatcgt accaatctca aaaaagtatt tctaacagat tcagaattac accaattgaa
     1741 tgaacggatt gctaagagca attgtcaaaa tttctcagtc tatgccagaa aagttttgct
     1801 taatcccaat atgtcatttg tcacgattaa cactgatact tatgaacagt tagtgtttga
     1861 attgaggagg attggaaata acatcaatca aattgcgcgt gcgattaatc aaagccatct
     1921 gatttctcag aaccaattac aagaattgag taaaggagtc ggagagttaa ttaaggaagt
     1981 ggacaaagaa tttcaagtgg aggtgaaaag actgaaggag tttcatggta gttactaaac
     2041 actttgcgac gcacggaaaa aaatatcgta ggcgtttaat taagtatatt ctcaaccctg
     2101 ataaaacgga caatttgaaa ttggtatctg attttggcat gagcaattac ttggacttcc
     2161 ctagtcatgc agaaatggta gagatgtaca atgtcaactt cagcaataat gacaagttgt
     2221 acgaatccag aaacgaccga caagaaaaac accaacaaac tattcatgct cattacctca
     2281 ttcaatcatt ttctcctgaa gataatctga cacctgaaga aattaaccgc attggttatg
     2341 agatcatgat ggaattgaca ggaggtcgtt ttaagtttat cgtagcaact cacacagaca
     2401 aagatcatgt tcataatcac atcctaatca acgctattga ccgcaattca gataaaaagt
     2461 tgatatggaa ctatgccttg gaacgaaacc tccgtatgat ttcagaccgt atttctaaag
     2521 tgactggtgc aaaaattatt gagaaacgtt attcctatcg agattataaa aaatataggg
     2581 aatctagtca taagtttgaa ttgaaacaac gtctttattt tttgctacag caatctaaat
     2641 cgtttgatga ttttttagag aaagcaaagc agttgcatgt tcagattgat tttagtcaaa
     2701 agcatagccg ttttttgatg acagatagaa cgatgataaa accaattcga ggacgccaac
     2761 tcagcaaacg agatttatat gatgaagact tttttaaaac atattttgcc aaacaagaga
     2821 ttgaaagtcg tttaaaattt ttactaaagt ctgtttattc attagaagaa ttgcatgtaa
     2881 aggctaaaga gttgaatcta accatcgagt taaaacagaa aaatgtgatg tttaccctag
     2941 aagaagatgg caaaaagata agcctgagtc ataaaaaaat tagcgataag aaattgtacg
     3001 atgttcaatt ttttaatcgt tattttgaag atagagaagt tagagatatt caagcattag
     3061 aaaatcttca ggaagatttt cagacttttc gagaggagca gcataaggag aaagtctccg
     3121 ctgaagaaat cgaggaagcc ttcaagaaat acaaggaaaa acgagatgct atccatgagt
     3181 ttgagattga acttacggat aatcagatag agaagttggt tgatgatggt gtctatatac
     3241 aggtttcgtt tggtatcaaa cagagtggac tcatctttat tcctaactac cagctagata
     3301 tttttgagga agacaatcat aaaaaatata aagtctacat tcgagaaacg tcttcttact
     3361 ttgtctataa taaagaaaac atggataata attgtttcat caagggtagg accttaatca
     3421 gacaattgag caacgatagc caaaaactcc cctatagacg accaacttta aaaagtctta
     3481 aagagaagat ttctgaaatt aatcttatga ttgaactatc taacacgaac aaacaatacc
     3541 aagaaatcaa agatgagctt gtattagaaa ttgcagagat tgatatgcag ctagaagaaa
     3601 cccaagaaaa aatcgccacc ttaaacaaga tggcggaagt ccttatcaat ttgaagagtg
     3661 aagatcatga gactagaaaa ttagctaagt atgattttga ccaaatgaac atgacggaat
     3721 caataatgtt agatcgactc aatacggata ttttaaaatt acaacaggaa cttggtaatg
     3781 aaattaataa atatgaggag atagctagaa gattggattt gtttgtgaaa attattaata
     3841 ccaataaatt tacagttcta aaatttcacg ag
//
LOCUS       DAVMGZ010000137         3723 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000137 DAVMGZ010000000
VERSION     DAVMGZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.138"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            74..763
                     /gene="coaB"
                     /locus_tag="VYQ93_001813"
     CDS             74..763
                     /gene="coaB"
                     /locus_tag="VYQ93_001813"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV3336455.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            775..1326
                     /gene="coaC"
                     /locus_tag="VYQ93_001814"
     CDS             775..1326
                     /gene="coaC"
                     /locus_tag="VYQ93_001814"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV3336456.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            1310..1873
                     /locus_tag="VYQ93_001815"
     CDS             1310..1873
                     /locus_tag="VYQ93_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3336457.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            2156..2683
                     /locus_tag="VYQ93_001816"
     CDS             2156..2683
                     /locus_tag="VYQ93_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3336458.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPIVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            2695..3210
                     /locus_tag="VYQ93_001817"
     CDS             2695..3210
                     /locus_tag="VYQ93_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV3336459.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(3207..3674)
                     /locus_tag="VYQ93_001818"
     CDS             complement(3207..3674)
                     /locus_tag="VYQ93_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV3336460.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVIYYKATEFSGSLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
BASE COUNT         1106 a          806 c          662 g         1149 t
ORIGIN      
        1 ataaatcttc gtttttacca aaaatttatc actttcattt tacttaccgc ttatttttgt
       61 gtacaatagt gctatgaaaa ttttagttac atcgggcggt accagtgaag ctatcgatag
      121 cgtccgctct atcactaacc attctacagg tcacttgggg aaaattatca cagagacttt
      181 gctttttgca gggcatgaag tttgtttaat tacgacaaaa cgagctctga agccagagcc
      241 tcatcctaac ctaagtattc gagaaattac caataccaag gaccttctaa tagaaatgca
      301 agaacgtgtt caggattatc aggtcttgat ccactcaatg gctgtttctg actacactcc
      361 tgtttatatg acagggcttg aggaagttca ggctagctcc aatctaaaag aatttttaag
      421 caagcagaat catcaggcca agatttcttc aactgatgag gttcaggttt tgttccttaa
      481 aaagacaccc aaaatcatat ccctagtcaa ggaatggaat cctactattc atctgattgg
      541 tttcaaactg ctggttgatg ttaccgaaga tcatctggtt gacattgcac gaaaaagtct
      601 tatcaagaat caagcagatt taatcatcgc gaatgacctg actcaaattt cagcaggtca
      661 gcaccgagct atatttgttg agaaaaatca gcttcaaaca gtccagacta aagaagaaat
      721 tgcagaactc ctccttgaaa aaattcaagc ctatcattct tagaaaggaa aactatggca
      781 aacattctct tggctgtaac gggttcaatc gcctcttata agtcggcaga tttagtcagt
      841 tctctaaaaa aacaaggcca tcaagtcact gtcttaatga ctcaggctgc tacagagttt
      901 atccaacctt tgacactaca ggtactctca cagaatcctg tccacttgga tgtcatgaag
      961 gaaccctatc ctgatcaggt caatcatatc gaacttggaa aaaaagcaga tttatttatc
     1021 gtggtacctg caactgctaa cactattgca aaactagctc acggatttgc ggacaacatg
     1081 gtaaccagta cagctctagc cctaccaagt catattccca aactaatagc tcctgctatg
     1141 aatacaaaaa tgtatgacca tccagtaact cagaataatc tgaaaacatt agaaacttac
     1201 ggctatcagc tgattgctcc taaggaatcc ctactagctt gtggagacca cggacgagga
     1261 gctttagctg acctcacaat tattttagaa agaataaagg aaactatcga tgaaaaaacg
     1321 ctctaatatt gcacccattg ctatcttttt tgctaccatg ctcgtgatac actttctgag
     1381 ctcacttatc tttaaccttt ttccatttcc aatcaaaccg accattgttc atattcctgt
     1441 cattattgcc agcattattt atggtccacg agttggggtt acacttggat ttttgatggg
     1501 attacttagc ttgacggtta acacgattac gattctaccg acaagctacc tcttctctcc
     1561 cttcgtacca aacggaaaca tctactcagc tatcattgcc attgtcccac gtattttgat
     1621 tggtttaact ccttacttag tctataaact gatgaaaaac aagactggtc tgattttagc
     1681 tggggccctt ggttccttga caaatactat ctttgtcctt ggaggaatct tcttcctatt
     1741 tggaaatgtt tataatggaa atatccaact tcttctggca accgttatct caacaaattc
     1801 aattgctgaa ttggtcattt ctgcaattct aaccctagcc attgttccac gactacaaac
     1861 cttgaaaaaa taaaaacaat ctccgctttc aaacctgaag gtggagattt tgctctatac
     1921 agtttctttt taatgaaatc tttactatta gtaaaacgaa ataagaacag gacaaatcga
     1981 tcaggacagc caaatcgatt tctaacaatg ttttagaagt agaggtggac tattctagtt
     2041 tcaatctact ataactttta actaaaaaat gatctataaa cccaagtaaa ttcttgcttt
     2101 attgtctggt ttattgtacc atactagtgt atatgcagtt aaaaaggaga ttcttgtgaa
     2161 tacacggaaa aagacacaat ttatgacaat gacagccctt ttaacggcta ttgcgatttt
     2221 gattccaatt gttatgcctt tcaagattgt cattccacct gcttcctata ctttggggag
     2281 ccacatcgct atttttatag ccatgttctt gtcgcccttg atggcagttt ttgtcatcct
     2341 agcctctagt tttggatttt tgatggctgg ctatcccatt gttatcgttt ttcgggcctt
     2401 ttcccatata tcttttggtg ctttaggagc tctttaccta caaaaattcc ccgataccct
     2461 agataaacca aaatcttcct gggttttcaa ttttgttttg gctgttgttc atgcccttgc
     2521 tgaagtatta gcctgtgtcc ttttttacgc aacttcgggt accaatgtag aaaacatgtt
     2581 ctatgttcta tttgtactag ttggatttgg cacaattatc catagtatgg tagactatac
     2641 attagcacta gctgtctata aagtgcttcg aaaacgccgt taaaaggaaa agctatgaca
     2701 aaagatcgca aacaagccct gctccaactc ttgaaagaag ctcctaaagc ccttaatggc
     2761 caaagtttgg cggaacattt tcatgtcaca cgtcaggtca ttgtgcagga cattgcaatt
     2821 ttgagagccg atggcgctcc tatcctatcc accaatcgtg gctacatcta taagcaaatt
     2881 gataccaatc cttatgttca caaacttttc aaagtgaaac atgaagttga agaaatcggt
     2941 caggaactcc ttgctattgt cgataatggt gggcatgtcc agaatacctt gattgaccat
     3001 cccgtttatg gagaaattga aaccttgctt aaactgtctt gtcgcagaga tgtgcaacat
     3061 tttctagaac aagtcgagca ttctgacttt agacctctat ctgaattgac agatggcatc
     3121 cattaccacc tagtcgaagc cgaaacacaa caagacctcc actatatcga ggaggccttg
     3181 gatcagctag gttatttagt aaaagactag aagattttct tttcccaatc gtcttctgta
     3241 cggcgaggat agaaaaactc tgacttgtcg ggagcttcca tcatttccat caatggtagc
     3301 atatcatagg ccagatccaa gtttggaatc tggtcttttt gcacccaaga aacttctcct
     3361 tcatctgaag accgaaggct accagagaac tcagtcgcct tataataaat gacaatgtag
     3421 cgcccacctg tatctagtgg ccaattttta atgccgacaa gttgaggatt ttggatagtc
     3481 aaccctgttt cttcatagat ttcacgaatg acagactctg cgaaagactc gccattttct
     3541 acatgacctc caggaaaggc ataaccagac cagcgattgt tttcaggggc gcgatactgc
     3601 atcaccacgc gctgagtttc gaggtcttca atcagacaga tatttgttaa aattgttaat
     3661 tgggaacggg acataaattt actcgctttc taatttatta aattttcaga cttactacca
     3721 gag
//
LOCUS       DAVMGZ010000138         3721 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000138 DAVMGZ010000000
VERSION     DAVMGZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.139"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            24..242
                     /gene="nrdH"
                     /locus_tag="VYQ93_001819"
     CDS             24..242
                     /gene="nrdH"
                     /locus_tag="VYQ93_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV3336461.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            323..2482
                     /gene="nrdE"
                     /locus_tag="VYQ93_001820"
     CDS             323..2482
                     /gene="nrdE"
                     /locus_tag="VYQ93_001820"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV3336462.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDEEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            2670..3632
                     /gene="nrdF"
                     /locus_tag="VYQ93_001821"
     CDS             2670..3632
                     /gene="nrdF"
                     /locus_tag="VYQ93_001821"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV3336463.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
BASE COUNT         1146 a          807 c          701 g         1067 t
ORIGIN      
        1 aaattttgaa gaggagttac acaatggtaa ccgtttattc taaaaacaat tgtgtccaat
       61 gtaaaatgac caagcgtttc ttggacagta ataatgtctc ttatcgtgaa atcaatcttg
      121 atgagcaacc tgagtacgtc gatcaagtta aagagctcgg ttttagcgca gctcctgtta
      181 tccaaacacc aactgaagtc ttttcaggtt tccaaccagg aaaactgaaa caattagcat
      241 aatcttagta catcatccag aagaaactgc ttttagggct aacttagaag cctttctttt
      301 gtaattagat aagggaaatt ttatgggatt aaaacatctt gaagacgtga cttacttccg
      361 tcttaataac gaaattaacc gtcctgttaa tggacaaatc atgcttcata aagatgagga
      421 agccttggat gctttcttta aagaaaatgt agttccaaac actatggttt ttgattcaat
      481 caaagacaaa atcaattacc tcattgaaca caactacatc gaaacagcct ttatcaagaa
      541 ataccgtcca gagttcttgg aagaattggc tcaatttatc aaagaccaaa acttccaatt
      601 caagtcattc atggcagctt ataaatttta caatcaatat gccttaaaga ctaatgacgg
      661 tgaatactat cttgaaaata tggaagaccg tgtcttcttc aacgcccttt atttcgctga
      721 tgggaatgaa gctgttgcaa tcgatattgc caatgaaatc atccaccaac gctaccaacc
      781 tgctactcct tccttcttga atgctggacg tgctcgtcgt ggggagttgg tatcttgttt
      841 cctaatccaa gtgacggatg atatgaactc tatcggacgt tctatcaact cagctcttca
      901 actttcacgt attggtggtg gtgtgggaat taccctcagc aaccttcgtg aagctggtgc
      961 acctatcaaa ggctatgaag gagctgcttc aggtgtcgtt cctgttatga agctttttga
     1021 agacagcttc tcttactcca accaattggg gcaacgtcaa ggtgccggcg ttgtctacct
     1081 caacgtcttt cacccagata tcatcgcttt cctttcaact aagaaagaaa acgctgatga
     1141 aaaagtacgt gtcaagactc tatcacttgg tgttgtagta cctgataaat tctacgaatt
     1201 ggctcgtaaa aatgaagaaa tgtacctctt cagcccttac tctgtagaaa aagaatacgg
     1261 tgtgccattc aattacatcg acattactga aaaatacgat gaattggtcg caaatccaaa
     1321 tattcgcaag acaaaaatca aggcgcggga tttggaaact gaaatctcta aattgcaaca
     1381 agagtctggc tatccttatg tagtcaacat cgatacggct aaccgtgcaa atcctgtcga
     1441 tggaaagatt atcatgagta acttgtgttc tgagattctt caagttcaag aaccaagctt
     1501 gatcaacgat gctcaagaat tccttcaaat gggaacagat gtttcatgta acctgggttc
     1561 aaccaacgtg gttaacatga tgacttcacc tgattttggt cgttctatcc gtgctatggt
     1621 tcgtgccctt actttcgtta cagatagttc acatatcgta gctgtaccaa cgattgacca
     1681 tggaaatagc caagcccaca cctttggtct cggtgccatg ggactgcaca gctaccttgc
     1741 ccaacaactc attgaatatg gttcacctga gtctgttgaa tttacaagca tctactttat
     1801 gcttatgaac tactggacct tggtagagtc aaacaatatt gcgcgtgaac gtggtattac
     1861 cttccacaac tttgaaaaat cagactatgc taacggaagt tactttgaca agtatgtaac
     1921 tggtgaattt gttccaacat cagaccgtgt taaagaactc ttcaaaaatg tttttatccc
     1981 tggtgttgct gattggactg aacttcgcga caaggttcaa gaggatggtc tttaccacca
     2041 aaatcgcctt gctgtagcgc caaatggttc tatcagctac atcaacgatg tttctgcttc
     2101 tatccacccg attacgcaac gtattgaaga acgtcaggaa aagaaaattg gtaaaatcta
     2161 ctaccctgct gctggcttgt ctacagaaac cattccttac tacacttctg cctacgatat
     2221 ggatatgcgt aaagtcatcg atgtttacgc tgctgcgact gaacacgtgg atcaaggact
     2281 ttcactcacc ctcttcatgc gtagtgacat tccaaaaggc ctttacgaat ggaagaggga
     2341 aaataaacaa acgacacgtg acttgtccat ccttcgtaac tatgccttta acaagggaat
     2401 caagtctatc tactacgtcc gtacctttac agatgacggt ggagaagtcg gcgccaacca
     2461 atgtgaaagc tgtgtgattt agtttctagc atagaaacac tatttttccc taagcagcac
     2521 tgataaaatc aaatctacta tgtcataaca taatttatgt aacattttaa aagtcgggct
     2581 tccgactttt tgtgcgatac tcctatagaa ttatggtaaa gtagaaataa ttgtgagttc
     2641 gcaatactaa acacagaaag agattttaaa tggaaactta ctacaaagcc attaactgga
     2701 atgccatcga agatgtcatc gacaaatcaa cttgggaaaa actgacggag caattttggc
     2761 tcgatacacg tattccctta tcaaacgact tggatgactg gagaaagcta tctaacaaag
     2821 agaaagactt ggtcggaaaa gtttttggtg gtttaaccct tcttgacact atgcaatctg
     2881 aaactggggt tcaagccctt cgcgcagaca tccgtacacc acatgaggaa gctgttttca
     2941 ataacatcca atttatggaa tctgtccacg ctaaatctta ctcatcaatc ttttctacct
     3001 tgaatactaa ggctgagatt gaagaaattt tcgaatggac caataccaat ccttacctac
     3061 aaaagaaggc tgagattgtc aacgaaatct acctaaacgg cagcccactt gaaaagaaag
     3121 ttgccagcgt cttcctcgaa accttcctct tctactctgg tttcttcact cccctctact
     3181 atctcggtaa caacaaactt gccaacgttg cagaaatcat taaattgatt attcgtgacg
     3241 agtctgttca cggaacctac attggttaca aattccaact tggtttcaat gaattacctg
     3301 aagaagagca agaaaaactc aaagaatgga tgtacgacct actctatact ctttatgaaa
     3361 atgaagaagg ctacacagag agtctttatg acggtgttgg ttggactgaa gaggttaaaa
     3421 ccttccttcg ctacaatgcc aataaagctc tcatgaacat gggacaagat ccacttttcc
     3481 cagattcagc tgaagatgtc aacccaatcg ttatgaacgg tatttcaaca ggaacttcca
     3541 accatgactt cttctctcaa gtcggaaatg gttaccttct tggcgaagtt gaagccatgc
     3601 aagatgatga ctacaactac ggtttggact aatactcttc gaaaatcaaa ttcaaaccac
     3661 gtcagcttcg ccttgccgta ctcaagtaca gcctgcggct agcttcctag tttgctcttt
     3721 g
//
LOCUS       DAVMGZ010000139         3706 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000139 DAVMGZ010000000
VERSION     DAVMGZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.140"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            158..385
                     /locus_tag="VYQ93_001822"
     CDS             158..385
                     /locus_tag="VYQ93_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336464.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            673..3555
                     /gene="nanA"
                     /locus_tag="VYQ93_001823"
     CDS             673..3555
                     /gene="nanA"
                     /locus_tag="VYQ93_001823"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV3336465.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPKGRVRLYVNGVLSRTSLRSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NERAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKDLISPTEAKVKRTREMGKGVIG
                     LEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKGSIESM
                     HNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLVTLTTK
                     EDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1264 a          621 c          838 g          983 t
ORIGIN      
        1 gttttaaaaa tgaggtataa taaatttact aacgcttata aaaagtgata gaatctattt
       61 ttatgtatat ttaaagatag attgctgtaa aaatagtagt agctatgcga aataacagat
      121 agagagaagg gattgaagct tagaaaaggg gaataatatg atatttaagg cattcaagac
      181 aaaaaagcag agaaaaagac aagttgaact acttttgaca gtttttttcg acagttttct
      241 gattgattta tttcttcact tatttgggat tgtccccttt aagctggata agattctgat
      301 tgtgagcttg attatatttc ccattatttc tacaagtatt tatgcttatg aaaagctatt
      361 tgaaaaagtg ttcgataagg attgagcagg aagtatggtg taaatagcat aggctgatgt
      421 ccatcatttg cttataaaga gatattttag tttaattgca gcggtgtcct ggtagataaa
      481 ctagattggc aggagtctga ttggagaaag gagaggggaa atttggcacc aatttgagat
      541 agtttgttta gttcattttt gtcatttaaa tgaactgtag taaaagaaag ttaataaaag
      601 acaaactaag tgcattttct ggagtaaatg tcttatttca gaaatcggga tatagatata
      661 gagaggatca gtatgaatcg gagtgttcaa gaacgtaagt gtcgttatag cattaggaaa
      721 ctatcggtag gagcggtttc tatgattgta ggagcagtgg tatttggaac gtctcctgtt
      781 ttagctcaag aaggggcaag tgagcaacct ctggcaaatg aaactcaact ttcgggggag
      841 agctcaaccc taactgatac agaaaagagc cagccttctt cagagactga actttctggc
      901 aataagcaag aacaagaaag gaaagataag caagaagaaa aaattccaag agattactat
      961 gcacgagatt tggaaaatgt cgaaacagtg atagaaaaag aagatgttga aaccaatgct
     1021 tcaaatggtc agagagttga tttatcaagt gaactagata aactaaagaa acttgaaaac
     1081 gcaacagttc acatggagtt taagccagat gccaaggccc cagcattcta taatctcttt
     1141 tctgtgtcaa gtgctactaa aaaagatgag tacttcacta tggcagttta caataatact
     1201 gctactctag aggggcgtgg ttcggatggg aaacagtttt acaataatta caacgatgca
     1261 cccttaaaag ttaaaccagg tcagtggaat tctgtgactt tcacagttga aaaaccgaca
     1321 gcagaactac ctaaaggccg agtgcgcctc tacgtaaacg gggtattatc tcgaacaagt
     1381 ctgagatctg gcaatttcat taaagatatg ccagatgtaa cgcatgtgca aatcggagca
     1441 accaagcgtg ccaacaatac ggtttggggg tcaaatctac agattcggaa tctcactgtg
     1501 tataatcgtg ctttaacacc agaagaggta caaaaacgta gtcaactttt taaacgctca
     1561 gatttagaaa aaaaactacc tgaaggagcg gctttaacag agaaaacgga catattcgaa
     1621 agcgggcgta acggtaaacc aaataaagat ggaatcaaga gttatcgtat tccagcactt
     1681 ctcaagacag ataaaggaac tttgatcgca ggtgcagatg aacgccgtct ccattcgagt
     1741 gactggggtg atatcggtat ggtcatcaga cgtagtgaag ataatggtaa aacttggggt
     1801 gaccgagtaa ccattaccaa cttacgtgac aatccaaaag cttctgaccc atcgatcggt
     1861 tcaccagtga atatcgatat ggtgttggtt caagatcctg aaacaaaacg aatcttttct
     1921 atctatgaca tgttcccaga agggaaggga atctttggaa tgtcttcaca aaaagaagaa
     1981 gcctacaaaa aaatcgatgg aaaaacctat caaatcctct atcgtgaagg agaaaaggga
     2041 gcttatacca ttcgagaaaa tggtactgtc tatacaccag atggtaaggc gacagactat
     2101 cgcgttgttg tagatcctgt taaaccagcc tatagcgaca agggtgatct atacaagggt
     2161 aaccaattac taggcaatat ctacttcaca acaaacaaaa cttctccatt tagaattgcc
     2221 aaggatagct atctatggat gtcctacagt gatgacgacg ggaagacatg gtcagcgcct
     2281 caagatatta ctccgatggt caaagccgat tggatgaaat tcttgggtgt aggtcctgga
     2341 acaggaattg tacttcggaa tgggcctcac aagggacgga ttttgatacc ggtttatacg
     2401 actaataatg tatctcactt aaatggctcg caatcttctc gtgtcatcta ttcagatgat
     2461 catggaaaaa cttggcatgc tggagaagcg gtcaacgata accgtcaggt agacggtcaa
     2521 aagatccact cttctacgat gaacaatgaa cgtgcgcaaa atacagaatc aacggtggta
     2581 caactaaaca atggagatgt taaactcttt atgcgtggtt tgactggaga tcttcaggtt
     2641 gctacaagta aagacggagg agtgacttgg gagaaggata tcaaacgtta tccacaggtt
     2701 aaagatgtct atgttcaaat gtctgctatc catacgatgc acgaaggaaa agaatacatc
     2761 atcctcagta atgcaggtgg accgaaacgt gaaaatggga tggtccactt ggcacgtgtc
     2821 gaagaaaatg gtgagttgac ttggctcaaa cacaatccaa ttcaaaaagg agagtttgcc
     2881 tataattcgc tccaagaatt aggaaatggg gagtatggta tcttgtatga acatactgaa
     2941 aaaggacaaa atgcctatac cctatcattt agaaaattta attgggactt tttgagcaaa
     3001 gatctgattt ctcctaccga agcgaaagtg aagcgaacta gagagatggg caaaggagtt
     3061 attggcttgg agttcgactc agaagtattg gtcaacaagg ctccaaccct tcaattggca
     3121 aatggtaaaa cagcgacttt cctaactcag tatgatagca agaccttgtt gtttgcagta
     3181 gataaggaag atatcggaca ggaaattatt ggtatagcta aaggaagcat cgaaagtatg
     3241 cataatcttc ctgtaaatct agcaggtgcc agagttcctg gcggagtaaa tggtagcaaa
     3301 gcagcggtgc atgaagttcc agaatttaca gggggagtta atggtacaga gccagctgtt
     3361 catgaaatcg cagagtataa gggatctgat tcgcttgtaa ctcttactac aaaagaagat
     3421 tatacttaca aagctcctct tgctcagcag gcacttcctg aaacaggaaa caaggagagt
     3481 gacctcctag cttcactagg actaacagct ttcttccttg gtctgtttac gctagggaaa
     3541 aagagagaac aataagagaa gaattctaaa catttgattt tgtaaaaata gaaggagata
     3601 gcaggttttc aagcctgcta tctttttttg atgacattca ggctgatacg aaatcataag
     3661 aggtctgaaa ctactttcag agtagtctgt tctataaaat atagta
//
LOCUS       DAVMGZ010000140         3670 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000140 DAVMGZ010000000
VERSION     DAVMGZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.141"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            60..731
                     /locus_tag="VYQ93_001824"
     CDS             60..731
                     /locus_tag="VYQ93_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV3336466.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            715..1533
                     /locus_tag="VYQ93_001825"
     CDS             715..1533
                     /locus_tag="VYQ93_001825"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV3336467.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            1530..2426
                     /locus_tag="VYQ93_001826"
     CDS             1530..2426
                     /locus_tag="VYQ93_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV3336468.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            2470..3444
                     /gene="pta"
                     /locus_tag="VYQ93_001827"
     CDS             2470..3444
                     /gene="pta"
                     /locus_tag="VYQ93_001827"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV3336469.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         1099 a          677 c          813 g         1081 t
ORIGIN      
        1 aatctggaga aaaaatcaaa gatatttttg acggataata tgagaacaag ggagaatata
       61 tgaccttaga atgggaagaa tttctagatc cttacattca agctgttggt gagttaaaga
      121 ttaaacttcg tggtattcgt aagcaatatc gtaagcaaaa taagcattct ccaattgagt
      181 ttgtgaccgg tcgagttaag ccaattgaga gcatcaaaga aaaaatggct cgtcgtggca
      241 ttacttatgc gaccttggaa cacgatttgc aggatattgc tggcttacgt gtgatggttc
      301 agtttgtaga tgacgtcaag gaagtagtgg atattttgca caagcgtcag gatatgcgaa
      361 tcatacagga gcgagattac attactcata gaaaagcatc aggctatcgt tcctatcatg
      421 tggtagtaga atatacggtt gataccatca atggagttaa gactattttg gcagaaattc
      481 aaattcgtac tttggccatg aatttctggg caacgataga acattctctc aactacaagt
      541 accaagggga tttcccagat gagattaaga agcgactgga aattacagct agaatcgccc
      601 atcagttgga tgaagaaatg ggtgaaattc gtgatgatat ccaagaagct caggcacttt
      661 ttgatccttt gagtagaaaa ttaaatgacg gtgtaggaaa cagtgacgat acagatgaag
      721 aatacaggta aacgaattga tctgatagcc aatagaaaac cgcagagtca aagggttttg
      781 tatgaattgc gagatcgttt gaagagaaat cagtttatac tcaatgatac caatccggat
      841 attgtcattt ccattggcgg ggatggtatg ctcttgtcgg cctttcataa gtacgaaaat
      901 cagcttgaga aggtccgctt tatcggtctt cacactggac atttgggctt ctatacagat
      961 tatcgtgatt ttgagttgga caagctagtg actaatttgc agctagatac tggggcaagg
     1021 gtttcttacc ctgttctgaa tgtgaaggtc tttcttgaaa atggtgaagt taagattttc
     1081 agagcactca acgaagccag catccgcagg tctgatcgaa ccatggtggc agatattgta
     1141 ataaatggtg ttccctttga acgttttcgt ggagacgggc taacagtttc gacaccgact
     1201 ggtagtactg cctataacaa gtctcttggc ggtgctgttt tacaccctac cattgaagct
     1261 ttgcaattaa cggaaattgc cagccttaat aatcgtgtct atcgaacact gggctcttcc
     1321 attattgtgc ctaagaagga taagattgaa cttattccaa caagaaacga ttatcatact
     1381 atttcggttg acaatagcgt ttattctttc cgtaatattg agcgtattga gtatcaaatc
     1441 gcccatcata agattcactt tgtcgcgact cctagccata ccagtttctg gaaccgtgtt
     1501 aaggatgcct ttatcggtga ggtggatgaa tgaggtttga atttatcgca gatgaacatg
     1561 tcaaggttaa gaccttctta aaaaagcacg aggtttctaa gggattgctg gccaagatta
     1621 agtttcgagg tggagctatt ctggtcaata atcaaccgca aaatgcaacg tatctattgg
     1681 acgttggaga ctacgttacc attgacattc ccgctgagaa aggctttgaa accttggagg
     1741 ctattgagct tccattagat attctctatg aggatgacca ctttctagtc ttgaataaac
     1801 cctatggagt ggcttctatt cctagtgtta atcactctaa taccattgcc aattttatca
     1861 agggttacta tgtcaagcaa aattatgaaa atcagcaggt tcacattgtt accagactag
     1921 atagggacac ttctggcttg atgctctttg ccaagcacgg ttatgcccat gcacgattag
     1981 acaagcagtt gcagaagaaa tctatcgaga aacgctactt tgctttggtt aagggagatg
     2041 gacatttgga accagaaggg gaaattattg ctccgattgc gcgtgatgaa gattccatta
     2101 ttaccagacg agtggctaaa ggcggaaagt atgcccatac ttcatacaag attgtagctt
     2161 cttatggaaa tattcacttg gtctatattc acctgcacac tggtcgaacc catcaaatcc
     2221 gagtccattt ttctcatatc ggttttcctt tgctgggaga tgatttgtat ggtggtagtc
     2281 tggaagatgg tattcaacgt caggctctgc attgccatta cctatccttt tatcatccat
     2341 ttttagagca agacttgcag ttagaaagtc ccttgccgga tgatttcagt aaccttatta
     2401 cccagttatc aactaatact ctataaaaac tgtctcagag tataattatt atcttaaagg
     2461 agaaaactca tggaagtttt tgaaagtctc aaagccaacc ttgttggtaa aaatgctcgt
     2521 atcgttctcc ctgaagggga agagcctcgt attcttcaag caacaaaacg cttagtaaaa
     2581 gaaacagaag tgattcctgt tttgcttgga aatcctgaaa aaattaaaat ttatcttgaa
     2641 attgaaggaa tcatggatgg ttatgaggtc atcgaccctc aacattatcc tcaatttgaa
     2701 gaaatggttt ctgccttggt ggagcgtcgc aagggcaaaa tgactgaaga agatgtacgc
     2761 aaggttttgg ttgaagatgt caactacttt ggtgtgatgt tggtttactt gggcttggtt
     2821 gatggaatgg tgtcaggagc gattcactca acagcttcaa cagttcgccc agctctacaa
     2881 atcatcaaaa ctcgtccaaa tgtaactcgt acttcaggag ccttcctcat ggttcgtggt
     2941 acggaacgtt acctatttgg agactgtgcc attaatatca atccagatgc agaagccttg
     3001 gctgaaattg ccatcaactc agcaatcaca gctaagatgt ttggcatcga acctaaaatt
     3061 gccatgttga gctattctac taaaggttca gggtttggtg aaagcgttga taaggtcgtt
     3121 gaagcaacta aaattgctca cgacttgcgt cctgaccttg aaatcgatgg tgagttgcaa
     3181 tttgatgcgg cctttgttcc cgaaactgca gctctgaaag ctccgggaag tacagtagct
     3241 ggtcaagcaa atgtcttcat cttcccaggt atcgaggcag gaaatatcgg ttacaagatg
     3301 gctgaacgcc tgggtggctt tgcggctgta ggacctgttt tgcaaggttt aaacaagcca
     3361 gttaatgatc tttctcgtgg atgtaatgca gatgatgttt acaagttgac cctcatcaca
     3421 gcagctcaag cagttcatca atagtgaaaa ctataaagtg atatactatg ctatactgta
     3481 gttatgaaac tatgtacgaa aagcactgcc attaattcct gagaactaaa ttactgattg
     3541 gtgtcaaaaa ggaaaacttc caagcgatga tatcctgtct atacacgacc tatagaaatc
     3601 tgtaatatac atatccgtaa aacgataaat tccctttttg attttaaatg agtatgaaaa
     3661 gagaattttt
//
LOCUS       DAVMGZ010000141         3659 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000141 DAVMGZ010000000
VERSION     DAVMGZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.142"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            52..732
                     /locus_tag="VYQ93_001828"
     CDS             52..732
                     /locus_tag="VYQ93_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3336470.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
     gene            692..1072
                     /locus_tag="VYQ93_001829"
     CDS             692..1072
                     /locus_tag="VYQ93_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV3336471.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESG"
     gene            1082..1369
                     /locus_tag="VYQ93_001830"
     CDS             1082..1369
                     /locus_tag="VYQ93_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3336472.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            1370..2127
                     /locus_tag="VYQ93_001831"
                     /pseudo
     CDS             1370..2127
                     /locus_tag="VYQ93_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            2139..3000
                     /locus_tag="VYQ93_001832"
                     /pseudo
     CDS             2139..3000
                     /locus_tag="VYQ93_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(3080..3634)
                     /locus_tag="VYQ93_001833"
     CDS             complement(3080..3634)
                     /locus_tag="VYQ93_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336473.1"
                     /translation="MIGITGANGSGKSVLFKLIAGLYSPSYGEVLINGENIVPERKIP
                     ANLGALIEEPGFINYYSGFKNLQYLASIRGVVGNQEISDTLKIVGLYEQKDQKVKTYS
                     LGMRKKLGIAQAIMENPSILLLDEPMNALDKSSVENMRTLFRKLSSEKGTTILIASHS
                     EEDIRILCDKVYAIEDKVCTLCSD"
BASE COUNT         1051 a          819 c          680 g         1109 t
ORIGIN      
        1 gattttaagt gctacaataa ctgtattatt tctagatggg aggttctatt tttggattgg
       61 tccattgttg aacaatatct accactatat caaaaggcat tctttctgac cttgcatatt
      121 gcagtttggg gaattttggg atcctttctg ctcggtttaa tcgttagtat catccgacat
      181 tatcgaatcc ctgttttggc gcaagtagcg acagcctaca ttgaattgtc acgtaatacg
      241 ccccttttga ttcaactctt ctttctctac ttcggtcttc cccgaatcgg gattgtccta
      301 tcttcagaag tctgtgcaac gcttggcctt gtctttttag gaggctccta tatggcagaa
      361 tctttccgaa gtgggctgga agccatcagt caaacccagc aggagattgg cctcgctatt
      421 ggtctgacac ctctacaggt ctttcgctat gtggttcttc cgcaagcaac agcggtggca
      481 ctcccctcct ttagtgccaa tgtcattttc cttatcaagg aaacctctgt tttctcagca
      541 gtggctttgg ccgacctcat gtacgtcgcc aaggatttga ttggtctcta ctatgagaca
      601 gacattgcgc tagctatgtt ggtagttgct tatctgatca tgctgctacc catctcactg
      661 gtctttagct ggatagaaag gaggacccgc catgcaggat tcgggaatcc aagtactctt
      721 tcaaggaaat aatctcctga gaatcttaca ggggttgggc gttacgattg ggatatccat
      781 cctgtctgtc ctcttatcca tgatgttcgg aacagtcatg ggaatcatca tgacctccca
      841 ttctagaatc atacgatttt taacacgatt gtatctggaa tttatccgta tcatgcccca
      901 gctggtgcta ctcttcatcg tttactttgg cttggctcga aactttaata tcaatatctc
      961 aggtgagact tcagctatta tcatttttac cctctgggga acagctgaaa tgggagactt
     1021 ggtacgtgga gctatcactt ctctccctaa acatcagttt gaaagtggat aggcactcga
     1081 cttgactaat gttcaacttt actaccacat catcatccca caagtcttaa gaagactgct
     1141 accgcaggct atcaatcttg tcactcggat gattaaaacc acttcattag ttgttttgat
     1201 tggggttgtg gaagtgacca aagttggaca acaaatcatc gatagcaatc gcctgaccat
     1261 cccaactgct tcattttgga tttatggaac cattctagtc ttatatttcg cagtttgcta
     1321 ccctatttcc aaactatcca ctcacttaga aaaacattgg agaaactaaa tgtctgaaac
     1381 tatcttagaa atcaaggaac taaaaaaatc cttcggagac aatcccatcc tccaaggact
     1441 ttctctagaa atcaaaaaag ggaagttgtt gtcatcctag ggccatctgg ttgtgggaaa
     1501 agtaccctcc ttcgttgcct caacggctta gaaagtattc aaggtggaga tatttttctg
     1561 gatggtcagt ctatcgttga aaataaaaaa gattttcacc tagttcgcca aaagattggc
     1621 atggtctttc aaagttatga actctttccc catctggatg tcttacaaaa cctcatccta
     1681 ggccctatca aagctcaagg aagggacaag aaagaagtaa cggaagaagc tttgcaatta
     1741 ctagagcgtg tcggtttgct ggataaacaa catagctttg cccgtcaatt atctggtgga
     1801 cagaagcaac gtgttgcaat tgtccgtgcc ctcctaatgc atccagaaat catccttttt
     1861 gacgaggtga ctgcttcgct ggatccagaa atggtgcgtg aggtgctgga acttatcaat
     1921 gatttggccc aagaaggccg taccatgatt ttagtaaccc acgaaatgca gtttgcccaa
     1981 gccattgctg accagattat cttcctcgac caagggaaaa tcgctgaaga aggaacagct
     2041 caagccttct ttaccaatcc gcaaaccaaa cgagcccagg aatttttaaa cgtctttgac
     2101 tttagccaat tcggctcata tctataaagg agattcttat gaaactattc aaaccactct
     2161 taactgtttt agcacttgcc tttgccctta tctttatcac tgcttgtagc tcaggtggaa
     2221 acgctggttc atcctctgga aaaacaactg ccaaagctcg cactatcgat gaaatcaaaa
     2281 aaaggcggtg aactgcgaat cgccgtgttt ggagataaaa aaccgtttgg ctacgttgac
     2341 aatgatggtt cttaccaagg ctacgatatt gaactaggga accaactagc taaagacctt
     2401 ggtgtcaagg ttaaatacat ttcagtcgat gctgccaacc gtgcggaata cttgatttca
     2461 aacaaggtag atattactct tgctaacttt acagtaactg acgaacgtaa gaaacaagtt
     2521 gattttgccc ttccatatat gaaagtttct ctgggtgtcg tatcacctaa gactggtctc
     2581 attacagatg tcaaacaact tgaaggtaaa accttaattg tcacaaaagg aacgactgct
     2641 gagacttatt ttgaaaagaa tcatccagaa atcaaactcc aaaaatacga ccaatacagt
     2701 gactcttacc aagctcttct tgacggacgt ggagatgcct tttcaactga caatacggaa
     2761 gttctagctt gggcgcttga aaataaagga tttgaagtag gaattacttc cctcggtgat
     2821 cccgatacca ttgcggcagc agttcaaaaa ggcaaccaag aattgctaga cttcatcaat
     2881 aaagatattg aaaaattagg caaggaaaac ttcttccaca aggcctatga aaaaacactt
     2941 cacccaacct acggtgacgc tgctaaagca gatgacctgg ttgttgaagg tggccattaa
     3001 ctcttaaaag ggactggatt ttaagctcca atcccttttt aagattttac ctataacatc
     3061 ctgagtctat ctaagatgtt caatctgaac acagtgtaca tactttatct tctattgcat
     3121 atactttatc acataagata cgaatatcct cttcactatg actagcaatc aaaattgttg
     3181 tccctttttc actagagagc tttctaaaca atgttctcat attttctaca cttgatttat
     3241 ccaaggcatt cataggttca tctagtaaaa gaatagaggg attctccata attgcttgag
     3301 caatccctag ctttttcctc atacctagcg aataagtttt aactttctgg tctttttgct
     3361 catatagacc aactattttc agtgtatcac tgatttcctg attaccaact actcctcgta
     3421 tgcttgccaa atattgtaaa ttcttaaagc cactataata atttataaaa ccaggttctt
     3481 caatcaaagc tcccaaatta gctggaattt ttctctcagg aacaatattt tccccattga
     3541 ttaacacttc tccataagac ggactatata aaccagctat taatttaaac aatacacttt
     3601 tccctgagcc attcgcacca gtaattccta taatttcccc ctgtttacaa ctaaaatta
//
LOCUS       DAVMGZ010000142         3642 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000142 DAVMGZ010000000
VERSION     DAVMGZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.143"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            107..2431
                     /gene="pflB"
                     /locus_tag="VYQ93_001834"
     CDS             107..2431
                     /gene="pflB"
                     /locus_tag="VYQ93_001834"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV3336474.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(2798..3016)
                     /locus_tag="VYQ93_001835"
     CDS             complement(2798..3016)
                     /locus_tag="VYQ93_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336475.1"
                     /translation="MMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(3112..3350)
                     /locus_tag="VYQ93_001836"
                     /pseudo
     CDS             complement(3112..3350)
                     /locus_tag="VYQ93_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3343..3627)
                     /locus_tag="VYQ93_001837"
     CDS             complement(3343..3627)
                     /locus_tag="VYQ93_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336476.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
BASE COUNT         1141 a          730 c          679 g         1092 t
ORIGIN      
        1 aagtgtaaaa ataggaattt ttcacttgtt gaaatcggtt actttatggt atacttgtct
       61 catgaatgta acagatgact gttactagaa aaaagaggac attaatatgg ttgttaagac
      121 agttgttgaa gcacaagata tttttgacaa agcttgggaa ggcttcaaag gcgtagattg
      181 gaaagaaaaa gcaagtgtat cacgctttgt acaagctaac tacacacctt atgatggaga
      241 cgaaagcttc cttgcaggac caacagagcg ttcacttcac atcaagaaaa ttgtagaaga
      301 aactaaagca cactacgaag aaactcgttt cccaatggac actcgtccaa catctatcgc
      361 tgatatccct gctggattta tcgacaaaga aaatgaagtt atcttcggta tccaaaacga
      421 tgaactcttc aaattgaact tcatgccaaa aggtggtatc cgtatggctg aaactacttt
      481 gaaagaaaat ggatacgaac cagacccagc tgttcacgaa atcttcacta aatatgtaac
      541 aacagttaac gacggtattt tccgtgccta cacttcaaat attcgtcgcg ctcgtcacgc
      601 acacactgta actggtcttc cagatgcata ctcacgcgga cgtatcatcg gtgtttacgc
      661 acgtcttgct ctttacggtg cagactactt gatgcaagaa aaagtaaacg actggaatgc
      721 aatcaaagaa atcgatgaag aaacaatccg tcttcgtgaa gaagtaaacc ttcaatacca
      781 agcattgcaa caagttgttc gcctgggtga cctttacggg gttgatgttc gcaaaccagc
      841 gatgaacgtg aaagaagcaa tccaatgggt taacattgct ttcatggctg tctgccgtgt
      901 gattaacggt gctgctacat ctctaggtcg tgtaccaatc gtattggaca tctttgcaga
      961 acgtgacctt gctcgtggta catttactga atcagaaatc caagaattcg ttgatgattt
     1021 cgttatgaaa cttcgtacag ttaaatttgc tcgtacaaaa gcttatgacc aattgtactc
     1081 aggtgaccca acctttatca caacttctat ggctggtatg ggtaacgacg gtcgtcaccg
     1141 tgttactaag atggactacc gtttcttgaa cactcttgac aacatcggta actcaccaga
     1201 accaaacttg acagttcttt ggactgacaa attgccatac aacttccgtc gctactgtat
     1261 gcacatgagc cacaaacact cttctatcca atacgaaggt gtaacaacaa tggctaaaga
     1321 cggatatggt gaaatgagct gtatctcatg ctgtgtgtct ccacttgatc cagaaaatga
     1381 agaacaacgc cacaacatcc agtacttcgg tgctcgtgta aacgttctta aagcccttct
     1441 tactggtttg aatggtggtt acgacgatgt tcacaaagac tacaaagtat ttgatatcga
     1501 accaatccgt gacgaagttc ttgaattcga atcagttaaa gcgaactttg aaaaatctct
     1561 tgactggttg actgacactt acgtagatgc cttgaacatc atccactaca tgactgatag
     1621 gtacaactac gaagctgttc aaatggcctt cttgccaact aaacaacgtg ccaacatggg
     1681 attcggtatc tgtggatttg ctaacactgt tgatacattg tcagctatca aatacgctac
     1741 agttaaacca atccgtgacg aagatggcta catctacgat tacgaaacaa tcggtgacta
     1801 cccacgctgg ggtgaagatg acccacgttc aaacgaattg gcagaatggt tgatcgaagc
     1861 ttacacaact cgtctacgta gccacaaact atacaaagac gcagaagcta cagtatcact
     1921 tttgacaatc acatctaacg ttgcttactc taaacaaact ggtaactcac cagttcacaa
     1981 aggtgtatac ctcaacgaag atggttctgt gaacttgtct aaacttgaat tcttctcacc
     2041 aggtgctaac ccatctaaca aagctaaagg tggttggttg caaaacttga actcactttc
     2101 tagccttgac tttagttatg cagctgacgg tatctcattg actacacaag tatcacctcg
     2161 tgctcttggt aagactcgtg atgaacaagt tgataacttg gtaacaatcc ttgatggtta
     2221 cttcgaaaac ggtggacaac acgttaactt gaacgttatg gacttgaacg atgtttacga
     2281 aaaaatcatg tcaggcgaag acgttatcgt acgtatctct ggatactgtg taaacactaa
     2341 atacctcact ccagaacaaa aaactgaatt gacacaacgt gtcttccacg aagttctttc
     2401 aatggatgac gccttggatg cattgagcta atcaagttct tgaataataa aaaggaaccc
     2461 tcggtcaaac gactgagggt tttgtgcttg ggatagtgtg agcaattcct tcggcgcaat
     2521 atgcaatgtt tttgggaagg aatagtggac cgaatctgaa atataataga ttgaaactag
     2581 aatagtacac ctttacttct aaaacattct tagaaatcga tttgactgtc ctgatcgatt
     2641 tgtcctattc ttatttcatt ttactatagt ccactgaaac gtctcaaaca gcgctagaaa
     2701 ttgactggaa ttctctaagt gatttgttcc gattttattt cattccatca taaaaataag
     2761 acgactggct aattgacaat catcttgttt gatactgtta ataaaattcg cgaagtttat
     2821 cttgattttc tttaaaaaat ttttctaacc aatccatacc ataagcataa ctaaaacctt
     2881 ctaatagaac agagaggtca atagtatcaa atcccatgtc ttttaaatat ttaaaggtat
     2941 cgaatggtga atgaaagttt ttgtatttaa taattgcacc aatatctttc aaatcctgct
     3001 ctctgatact catcatcttc attcctaaca catactcaat agggactact aaaacggtca
     3061 gattctcaaa tgagtacaag gattcgcata aacttaaggg aggttgtttg ttcatatttg
     3121 ctttgcaaca tgattgttga gccataactc ttcgtggatg ttaagattaa actgttttcc
     3181 tacacgagca ataatttcat tgattttctg attttggtca taaaaagcat caacatcttg
     3241 tgtggcacgt aaaccatgat attctaagac ataaccataa ttgttaaagt cagattttcc
     3301 tgaattaatt tcttatttag ggcttggaag accggtttca ttttatccat gtttgtattt
     3361 ctccatcaat ttttgaacat tggttaactt agcaggttta tttaatgaaa tcacatattt
     3421 atgagttgga gtatgctctt gaaaggcata cttcaagcta attttttgaa aacgggttag
     3481 ttctttggag tctagcaact tttctacatc ttggtttgtt aaataagaat agtcttcaaa
     3541 aataactttt cgcaaatagc gaaaccaatg gcttgcgttg aattgtgatt gtttggatgc
     3601 ctgtatcata taatcgtacc aatccattgt cttcctccta ta
//
LOCUS       DAVMGZ010000143         3621 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000143 DAVMGZ010000000
VERSION     DAVMGZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.144"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            143..1372
                     /gene="arcA"
                     /locus_tag="VYQ93_001838"
     CDS             143..1372
                     /gene="arcA"
                     /locus_tag="VYQ93_001838"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV3336477.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1430..2446
                     /gene="argF"
                     /locus_tag="VYQ93_001839"
     CDS             1430..2446
                     /gene="argF"
                     /locus_tag="VYQ93_001839"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV3336478.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2611..3558
                     /gene="arcC"
                     /locus_tag="VYQ93_001840"
     CDS             2611..3558
                     /gene="arcC"
                     /locus_tag="VYQ93_001840"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV3336479.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
BASE COUNT         1144 a          709 c          757 g         1011 t
ORIGIN      
        1 cataaaattg tgctaaacat cataatttat attattttcc gagaaattat agccattgac
       61 attacctagg aattgcgtta taacatacat gtaagcggta cccaaaatgg tgccaagtca
      121 aaatttttaa ggaggaaaat acatgtcttc tcatccaatt caggtcttct cagaaattgg
      181 gaaactgaaa aaagttatgt tgcaccgtcc aggcaaggag ttagaaaact tgttgccgga
      241 ctatcttgaa aggcttcttt ttgatgatat tcctttcttg gaagatgctc aaaaagaaca
      301 tgatgcattt gcccaagctc ttcgcgatga aggaattgag gttctctacc tagaacaact
      361 cgctgctgaa tcattgacct ctccagaaat ccgcgatcaa tttatcgagg aatacttaga
      421 cgaagccaac atccgtgatc gtcaaactaa ggttgctatt cgtgaattgc ttcacggcat
      481 caaggacaac caagaattgg ttgaaaaaac aatggctggg attcaaaaag ttgaattgcc
      541 agaaattcct gacgaagcta aagatctaac tgacttagtt gaatcagatt atccatttgc
      601 aattgacccg atgccaaacc tctatttcac tcgcgaccca tttgcaacaa ttggaaacgc
      661 cgtatcgctt aaccacatgt ttgcagacac tcgtaaccgt gaaacactct acggtaagta
      721 tatcttcaaa taccacccaa tctatggcgg aaaagtggat ttggtctaca accgtgaaga
      781 agatacgcgt atcgaaggtg gagacgagct agttctttct aaagacgtcc ttgcagtagg
      841 tatctctcaa cgtacagacg cagcttctat cgaaaaactt ttggtcaaca tcttcaagaa
      901 aaatgttggc ttcaagaaag ttttggcctt tgaatttgct aacaaccgta aattcatgca
      961 cttggatact gtcttcacta tggtagacta tgacaagttc actattcacc cagaaatcga
     1021 aggcgacctt cacgtttact cagttactta cgaaaacgaa aaacttaaaa tcgttgaaga
     1081 gaaaggtgac ttagctgaac ttcttgctca aaaccttggt gtagaaaaag ttcatttgat
     1141 tcgttgcggt ggtggcaata tcgtagcagc tgcgcgtgaa caatggaacg acggttctaa
     1201 cactttgacc atcgcacctg gtgtggtagt tgtttatgac cgcaataccg tgaccaataa
     1261 gattttggaa gaatacgggc ttcgcttgat taagattcgc ggaagtgaat tggttcgggg
     1321 ccgtggtgga cctcgttgta tgtctatgcc atttgaacgt gaagaagtgt aatcgctgtt
     1381 cggtattcgt caatagaaaa tgtaaaaaat agaaagagga aataataaaa tgacaaattc
     1441 agtattccaa ggacgcagct tcttagcaga aaaagacttt acccgtgcag agttagaata
     1501 ccttattggt ctttcagctc acttgaaaga tttgaaaaaa cgcaatattc aacaccacta
     1561 ccttgctggc aagaatatcg ctctcctatt tgaaaaaaca tctactcgta ctcgtgcagc
     1621 ctttacaact gcggctatcg accttggtgc tcacccagaa tacctcggag caaatgatat
     1681 tcagttgggt aaaaaagaat ctactgaaga tactgctaaa gtattgggac gtatgtttga
     1741 cgggattgaa ttccgcggat tcagccaacg tatggttgaa gaattggcag aattctcagg
     1801 cgttccagta tggaacggtc taactgacga atggcaccca actcaaatgc tcgctgacta
     1861 cttgactgtt caagaaaact tcggtcgctt ggaaggcttg acattggtat actgtggtga
     1921 tggacgtaac aacgttgcca acagcttgct cgtaacaggt gctatccttg gtgtcaatgt
     1981 tcacatcttc tcaccaaaag aactcttccc agaaaaagaa atcgttgaat tggcagaagg
     2041 atttgctaaa gaaagtggcg cacatgttct catcactgaa gatgctgatg aagcagttaa
     2101 agatgcagac gttctttaca cagacgtttg ggtatcaatg ggtgaagaag acaaattcgc
     2161 agaacgtgta gctcttctta aaccttacca agtcaatatg gacttagtta aaaaagcagg
     2221 caatgaaaac ttgatcttcc tacactgctt gccagcattc cacgatactc acactgttta
     2281 tggtaaagac gttgctgaaa aatttggtgt agaagaaatg gaagtaacag acgaagtctt
     2341 ccgcagcaag tacgctcgcc acttcgatca agcagaaaac cgtatgcaca ctatcaaagc
     2401 tgttatggct gctacacttg gtaaccttta tattcctaaa gtataatttt agataataaa
     2461 ccgtctacca acagctatga gggctgcgac taatagcttt agtccggtcc tcttttatgt
     2521 aatggtaatc tattatttct tataaaatat gtgaaaaatc attaaattga aatctaaacg
     2581 cattctattg agtgtgataa aggagaattt atggcaaatc gtaaaattgt agtagctttg
     2641 ggaggaaatg cgattctttc ttctgaccca tcagcaaagg ctcaacaaga agctttagtt
     2701 gaaacagcta agcatcttgt aaaattgatt aaaaatggag atgatctgat tatcactcac
     2761 ggtaatggac ctcaagttgg gaatctcttg ctccaacatt tggcatcaga ctctgaaaag
     2821 aaccctgcct tcccactcga ctcacttgtc gctatgacag aaggtagcat cggtttctgg
     2881 ttgaaaaatg ctttgcaaaa tgctctcttg gatgaaggca tcgaaaaaaa tgttgcctct
     2941 gttgtaacgc aagttgtcgt agataaaaat gatccagctt ttgttaactt gagtaaacca
     3001 atcggtcctt tctattcaga agaagaagca aaagcagaag ccgaaaagag cggagcgact
     3061 ttcaaggaag atgctggccg tggctggcgt aaggtcgttg cctcaccaaa acctgttgac
     3121 atcaaagaaa ttgaaaccat ccgtactctt ttaaataatg gtcaagtcgt cgtagctgca
     3181 ggtggtggcg gtattcccgt tgtcaaagaa aacaatggac atttgactgg tgtcgaagcg
     3241 gttattgata aagacttcgc ttcccaacgt ttggcagaat tggttgatgc agacctcttc
     3301 atcgttttga caggtgtaga ttatgtattt gttaactaca acaagccaaa ccaggaaaaa
     3361 ttggaacatg tgaatgttgc ccagctggaa gaatatatca aacaagatca gtttgcacca
     3421 ggtagcatgc ttccaaaagt agaagcagct atcgcttttg tcaatggtcg tccagaagga
     3481 aaagcagtta ttacttccct tgaaaatcta ggcgccttga ttgaatctga aagcggaaca
     3541 attattgaaa aaggataagt tgttttacta ataagatgta ttctatttct agtatcttta
     3601 tatcaaatta gaaattattc t
//
LOCUS       DAVMGZ010000144         3609 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000144 DAVMGZ010000000
VERSION     DAVMGZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.145"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            48..1823
                     /locus_tag="VYQ93_001841"
     CDS             48..1823
                     /locus_tag="VYQ93_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEV3336480.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            1824..3209
                     /locus_tag="VYQ93_001842"
     CDS             1824..3209
                     /locus_tag="VYQ93_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEV3336481.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            3232..>3609
                     /locus_tag="VYQ93_001843"
     CDS             3232..>3609
                     /locus_tag="VYQ93_001843"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEV3336482.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNS"
BASE COUNT         1147 a          544 c          803 g         1115 t
ORIGIN      
        1 tctgtaatat tattctataa tttttggact tagtaaggag aataactttg actcaaggga
       61 agattataaa agtatcggga cctctagtta ttgcatcagg tatgcaggag gctaatattc
      121 aagatatttg ccgtgtaggt aagctagggt taatcggtga aattattgaa atgagaagag
      181 atcaggcatc tatccaagtc tatgaagaaa catctggtct tggtccggga gaacctgttg
      241 ttacaactgg agaacctctc tcggttgaat tagggccagg attgatttct caaatgtttg
      301 atggcataca acgcccatta gatcgattta aattggctac tcataatgat tttctagttc
      361 gtggggtaga agttccaagt ttggatagag atattaagtg gcattttgat tccactatag
      421 caattggtca aaaagtgagt acgggtgata ttcttggaac tgtcaaggaa accgaggtag
      481 ttaatcataa aattatggtt ccttatggag tatctggaga agtcgtttct attgcatctg
      541 gcgattttac aattgatgaa gttgtatatg aaataaaaaa attggacggt agtttctata
      601 aaggaacgct tatgcaaaaa tggcctgtcc gcaaggcgcg tcctgtttct aaacgtttaa
      661 ttccagaaga accattaatc acaggtcaac gagttattga tgcattcttt ccagtaacca
      721 aagggggagc tgcagcagtt cctggaccgt ttggagcagg aaagacagtt gtacaacacc
      781 aagtagctaa atttgccaat gttgatattg ttatttatgt cggttgtgga gaacgtggaa
      841 atgaaatgac ggatgtactg aatgagtttc ctgagttgat tgaccctaat accggacaat
      901 caattatgca acggacagtt ctgattgcta atacttcaaa tatgcctgtt gctgctcgtg
      961 aggcttcaat ttatacagga attaccatgg ctgagtattt tcgtgatatg ggctactctg
     1021 tcgccattat ggctgattca acttcacgtt gggcagaagc gctacgtgaa atgtcaggac
     1081 gtctagaaga aatgcctggt gatgagggtt atcctgctta tctgggaagt cgtatcgctg
     1141 aatattatga aagagcagga cgttctcagg ttctagggct tccagaacgt gaaggaacga
     1201 ttactgctat tggagctgta tcgccacctg gtggagatat ttcagaacca gttactcaaa
     1261 acactttacg gattgtgaaa gttttttggg ggcttgatgc tccgttggca cagcgacgtc
     1321 attttcctgc aattaactgg cttacatctt attcactata taaagacagt gtgggcactt
     1381 atatagatgg taaagagaag acagattgga atagtaaaat aactcgtgcg atgaactact
     1441 tacaacggga atctagttta gaggaaattg ttcgtcttgt tggaattgat tctctgtctg
     1501 ataatgaacg actaacgatg gaaattgcta aacaaattcg agaagattat ttgcaacaga
     1561 acgcttttga ttcggtagat acattcactt cgtttgcaaa acaagaagca atgctaagta
     1621 atattctcac ttttgctgat caggcaaatc atgctttaga gttgggttct tactttacag
     1681 agattatgga aggtaccgtg gcagttcgag accgtatggc gagaagtaaa tatgtttcag
     1741 aagatagatt agatgaaatc aaaattatat caaatgagat tacacatcaa attcatttga
     1801 tattagaaac aggaggtcta taaatgagtg ttataaaaga atacagaact gctagtgaag
     1861 ttgttgggcc tcttatgatt gttgaacaag taaataatgt gtcttacaat gagttagttg
     1921 aaattcaact tcataatgga gaaattcgtc gtggacaagt tttagagatc cacgaagata
     1981 aagcaatggt tcagcttttt gaaggatcta gtggaataaa tttagaaaag tctaaaattc
     2041 gttttgctgg tcatgcatta gaattggctg tatctgagga tatggttggt cgtattttta
     2101 atgggatggg aaaaccaatt gatggtggac cagatttaat tccagagaaa tatttagata
     2161 ttgatggtca agctattaat cctgtatcta gagattatcc agatgaattt attcagacag
     2221 ggatctcctc tattgatcat ttgaatactc ttgtacgtgg tcaaaaatta ccagtatttt
     2281 caggttcggg cttacctcat aatgaattag ctgctcagat agcaagacaa gcgactgttt
     2341 taaattctga tgaaaatttt gcggttgtat ttgcagcaat gggtattact tttgaagaag
     2401 ctgagttttt tatggaagaa ctcagaaaaa caggagcgat cgatcgttcg gttttattta
     2461 tgaacttggc aaatgatcct gcaattgagc gtattgcaac tccccgcatt gctttaactg
     2521 cggcagagta tctagctttt gaaaaagata tgcacgttct agttatcatg acggatatga
     2581 ctaactattg tgaagcgtta cgtgaagtct cggcagctcg ccgtgaagtt ccagggagac
     2641 gaggctatcc gggatattta tatacaaatt tatcaactct atacgaaagg gctggtcgct
     2701 tagttggtaa aaaaggttcg gtgacacaga ttcctatttt aacaatgcca gaagatgaca
     2761 taacacatcc aattcctgat ttaactggat acattactga agggcaaatt attttgtcgc
     2821 atgagttgta taatcaaggt tatcgtccac caatcaatgt tttaccttct ctctctcgat
     2881 taaaagataa gggatctgga gaaggtaaaa ctcgtggaga tcatgctcca actatgaatc
     2941 aactgtttgc agcctatgcc caagggaaaa aggttgaaga gttagcagta gtattaggag
     3001 aatcggcttt atctgatgta gataaattgt atgtgaggtt tacaaagcgt tttgaagaag
     3061 agtacataaa ccaaggattt tataaaaatc gaaatataga agatacgttg aatcttgggt
     3121 gggaattact atcaattctt cctagaacag agttaaaacg tatcaaagat gatttgcttg
     3181 ataaatactt acctttggta gaagtttaat ccggaaatgg agtgattatc tatggtacgt
     3241 ttgaatgtaa aaccaactcg tatggaattg aataacttaa aggaacgttt gacaacagct
     3301 gaacgtggac ataagttatt aaaggataaa agagatgaat tgatgaggcg atttatttct
     3361 ttgattcgtg agaataatca acttcggaaa gaagtggaaa gttatctaat tgataatcta
     3421 aaatcctttg cagttgctaa atcattaaag aattctcaaa tggtggagga attattttca
     3481 attccatcga aagaaattga attatttgtt gagaaagaaa atatcatgag tgtaacagtt
     3541 cctagaatgc atatgaatat tacttctcaa aatgagaaca gtgaatacag ctatttatct
     3601 tctaatagt
//
LOCUS       DAVMGZ010000145         3597 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000145 DAVMGZ010000000
VERSION     DAVMGZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.146"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(134..1270)
                     /gene="lctO"
                     /locus_tag="VYQ93_001844"
     CDS             complement(134..1270)
                     /gene="lctO"
                     /locus_tag="VYQ93_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV3336483.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1895..3385)
                     /gene="lysS"
                     /locus_tag="VYQ93_001845"
     CDS             complement(1895..3385)
                     /gene="lysS"
                     /locus_tag="VYQ93_001845"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV3336484.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3512..>3597)
                     /locus_tag="VYQ93_001846"
     CDS             complement(3512..>3597)
                     /locus_tag="VYQ93_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV3336485.1"
                     /translation="PMAQQVIPKAAFGYIASGAEDTFTSFQ"
BASE COUNT         1028 a          769 c          708 g         1092 t
ORIGIN      
        1 tgttagaaat cgatttaact gtcctgattg atttgtccta ttcttatttc attttactat
       61 agttttcgat agcgatttat tcttccaata cacgaagaaa aacctccaca ttcagtgaag
      121 gcaatctgtt ttatcaatac aattttaagt cacgagggtc aactgggaag gttgggttgt
      181 atggattgtg acggagctta aagtgtttga catcttcaat ggtctgagct ccagacaatt
      241 gcataactgt cttcaattcc gcattcaagt gttcaaagac ttgacgcaca ccgacactac
      301 caccgagagc caagccataa ataacagggc gaccaatagc taccaagtct gctcctgaag
      361 ccaaggcttt aaagacgtgt tgaccacgac gaacaccaga gtcaaagaca atcggcacac
      421 gtctatcaac tgcttctgcc acttcttgaa gcgagtcaaa ggcagctggt ccaccgtcga
      481 tttgacgacc accgtggttg gttacccaga taccagaagc tcctgcagca agtgaacgtt
      541 caacgtcctc acggcattgt ggtcccttga catacacagg aagaccagag tattcagcga
      601 taaattctac atcgcgtgga gacaagcgtt gtttagctga tttgtaaaca aagtccattg
      661 atttaccagc accttctggc aggtattctt caacaatcgg catgccaact gggaagacaa
      721 aaccattacg cttatccact tcacgattcc cccctacagt agcatctgcc gtcaagacaa
      781 tcgctttata accttcagcc ttcacacggt ccatgatgtg gcggttgata ccgtcatcct
      841 tactaaagta aaattggaac caatggggtg tcccttgaag ggcttcagaa atctctggaa
      901 ggtcaacagt agagtaagag ctggttgtat aaagagaacc aaactcatgc acaccacgcg
      961 cagtcgccac ttccccctgt tcatttgcca atttatgagc cgcaacaggc gccataatga
     1021 ttggtgaaga tagtttttca cctgcaaatt caatctctgt acttggattt tcaacatcgc
     1081 aaagtgtatg aggaacgatg agtttgtggt taaaggcgcg gatattctct cttaaagtga
     1141 aagtatcttc cgccccacta gcgatatagc caaatgctgc tttaggaata acttgttgcg
     1201 ccattggctc caaatcatag gtattgatga agtctacatg accttctgca ttgcttgttt
     1261 tgtatgacat aaaatgccct ccttgattaa gtaagcgttt gctttttact acacaactat
     1321 aataactctt ttttcaatca ttttcaagag ttttgcttaa aaaccatctt attttttcat
     1381 acaatatact agccctactt tccttaaact ctaaacttta gtctatatta agtgaatcgt
     1441 tgagattttt tgctgccagg catttgaact acttttttat ttgcggattc tatctactaa
     1501 tactataaaa aaacaagtga ataagacacc agaacatcct ctaataggac ctatggactt
     1561 atatcaatcc catatatact gtttaagcaa gatactatcc tagttgacgc taacttccta
     1621 tcccacttat taatttttta tttatagtga atctgaacaa atcaattggg gaatccaagt
     1681 caatttctag tactttttga acagtttcag cgtactatcg taaaatgaaa taaaacatgc
     1741 acaaattgct tagggaattt aaaccaattt ctaacaatgt cttagaaatc aaagtgtact
     1801 atttcaactt caatatacta cacttcattt tctccttaaa ttcagtctct ttttgaatca
     1861 aaaaatcctc tggtaagagc cagaggataa gaatttattt cattgttggg aagagcaata
     1921 catcacggat agttgttgta tcagtgagga gcatgcagag acggtcgata ccgattccca
     1981 aaccacctgt tggtggcata ccgtattcaa gagcttcaat gtagtcatag tcgattcctg
     2041 tcgcttcatc atcaccaagt tctttggctt tagcttgggc ttcaaaacgg ctaagttggt
     2101 cgattgggtc gttgagttca gtaaaggcat taccgtactc cttagtcatg ataaagagct
     2161 cgaaacggtc agtaaagcgt tggtcttcag gatttttctt agcgagtgga gatacagcta
     2221 ctggatgtcc atagacaaag gttggttgga ttaaagtttc ttcaacaaac tcttcaaaga
     2281 aggcattgat gatgtgacca acctcagtgt agtgtttctc aactggaact ttcttctcag
     2341 cagcgatagc tttagcttct tctaaagtca tgtcttgcca gaaatcgaca ccagtaattt
     2401 ctctgatagc atccaccata tgaacacgct taaatggttc gttaatcttg atttcagtac
     2461 cttggtagtt gactgggcca tcgcccttaa ctgatttagc agcgtgttgg ataatgcctt
     2521 ccgtcaagtc catgatgtct tggaagtctg cataagcttg gtaaacttcg atagaagtaa
     2581 actcagggtt atgagtagcg tccattcctt cgttacggaa gatacggcca atttcataga
     2641 cacgttccat accacccacg ataaggcgtt ttaagtgaag ctcagtcgcg atacgaagca
     2701 ccatgtcaat gttttgggca ttgtggtggg tgataaatgg acgggcagca gcaccaccgg
     2761 cttcattatg aagaacaggt gtttccactt caaggaatcc tttttggtca aggtaacgac
     2821 ggatttcaga gatgattttt gaacgagtga caaagcgttc aaagctttca cgattagaaa
     2881 tcaagtcaag gtaacgttta cggtaaattg tttcaacatc tgtcaaacca tggaatttct
     2941 caggaagagg acgaagagcc ttagacaagt gtgtgatgtg ggttgccttg atagagagtt
     3001 ctcccatatc cgtacgcatc acttcacctt cgacaccaag gaagtcacca aggtctgctt
     3061 ttttgaagat ttcgtagttt tcttcaccga cagcatcctt acgaacgtag atctgaatct
     3121 ggccttcgcg gtcttgaagg tgggcaaaac caacttttcc tttaccacgt ttggttacca
     3181 agcgtcctgc gatagtagct gtttcgtttt tatcgtgtaa ttgttcttta tcgaggttgg
     3241 catatttatc ttttaattct tgtgaatttg cagtacgttc aaaacgtttt ccgaaaggat
     3301 cgattccttg ttcgcggagc gcagccattt tttcacggcg aacaatctgc tggtcattta
     3361 gttcttccat atgttctgta gacatgggat cctcctagtt ttactcaaaa ttgaatacga
     3421 tgagtcattt tttgcgatac cccattttat cataaataag caagaaattc acggataatc
     3481 tttaaaaact aaaaagaacc tgacgctaaa atcactggaa agaagtgaaa gtatcttccg
     3541 ccccactagc gatatagcca aatgctgctt taggaataac ttgttgcgcc attggct
//
LOCUS       DAVMGZ010000146         3575 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000146 DAVMGZ010000000
VERSION     DAVMGZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.147"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            436..1146
                     /gene="deoD"
                     /locus_tag="VYQ93_001847"
     CDS             436..1146
                     /gene="deoD"
                     /locus_tag="VYQ93_001847"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV3336486.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1169..1693)
                     /locus_tag="VYQ93_001848"
     CDS             complement(1169..1693)
                     /locus_tag="VYQ93_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV3336487.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1746..1982)
                     /locus_tag="VYQ93_001849"
     CDS             complement(1746..1982)
                     /locus_tag="VYQ93_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV3336488.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2050..2970)
                     /locus_tag="VYQ93_001850"
     CDS             complement(2050..2970)
                     /locus_tag="VYQ93_001850"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV3336489.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3160..3564
                     /locus_tag="VYQ93_001851"
     CDS             3160..3564
                     /locus_tag="VYQ93_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336490.1"
                     /translation="MKILKRYILELCFILSFALPFTKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFICSILIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
BASE COUNT         1040 a          623 c          732 g         1180 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaccccacc
      181 ctaaaagcag tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc
      241 tattttccct tcgctttttg acgggtttgg tatctttctc aatataaaat ataaaataaa
      301 gaaaggtaga gcgtgtgttt tgatttgaac acgagcggaa aactcggaaa atagataatc
      361 tgactgaaaa atcaggattt ctcgtcggct tcctaatttt cagtcgtttt cttctcgctc
      421 tttgtatcat aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa
      481 attcttcttc ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttgatgat
      541 gctgtttgtt ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac
      601 tgtgtatctg tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag
      661 ttaatcgtag actacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat
      721 gaagaggttc atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc
      781 gttcgtaatg actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat
      841 aaagcctacc atatcgccaa aaaacttggt atgactactc acgttgggaa cgttttgtca
      901 tctgatgtct tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc
      961 aaggctgtgg aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat
     1021 gcgctagcta tcatgaccat ctctgatagc ttggtcaatc cagacgaaga tacaactgca
     1081 gaagaacgtc aaaatacctt cactgatatg atgaaggttg gtttggaaac cttgattgca
     1141 gaataattat agccaaaaag gaagcccttt atgaattgta ggacttcctt ttcttatcca
     1201 gaaattgatc tagttctctc tgatttcgaa gaatagtgac tttatgtgaa tattcttggc
     1261 aaagtttttg gtaattttct ttttgagttt tgctacgccc atcccaaaga atccatctga
     1321 taaactccca ctcaaagcgt tcagggcaat ctgccgccat actttctctg acttttccac
     1381 ggtatttaag ataacgctta aaggctctaa agagacaggt caatggcaaa aaattgagaa
     1441 agatgatttg gtcagcttct tgcattcgtt cttggtagta gcaccaagaa taattaccat
     1501 cgatgaccca agctttatgc ttggtgagaa agttttttat ctcggttaac atccattcgc
     1561 agtcactgtc ttgccaacca ggttgaaatt ggagtgtgtc catgtgcagt tttggaatgg
     1621 agtagtagtt agataacttt tctgctagag ttgacttacc agaaccagaa tatccgataa
     1681 ttgcgatttt cattttctac cttttcctat ttggagacaa aaaaacagcc tctatggact
     1741 gtttcttatt tagcaagttt agctgaaaga cgagctttat cgcggcttgc tttgtttttg
     1801 tgaatcaaac ctttagtttc tgctttatcg atagctgagc tagcagcacg gaaaagttct
     1861 tcagatgggt ttgcttcgaa agcttttata gcagtacgca tagctgattt ttgagctgag
     1921 ttcttttcgt tttgtttaac gttcaattca gcgcgtttga tagctgattt aatgtttgcc
     1981 aatgttctta cctccatatt tactaactat accattatat atgaaaactt acgttttgac
     2041 aagggaaaat tactttttta agtaaatttc atcgatttca tggttcttgc ttttatgaag
     2101 aatcacttct gcacgatttc tggttggttc aatataattt tgcagatttg tgagattgat
     2161 actggtccag acctgatggg caaaggactc cacttcccca atcggcatct gagtaaaacg
     2221 ataatagtag ctatcagggt cgttttgggc taggctcagc attttcaaga aacggtccag
     2281 ataccaactt tcaatatcat ccactccagc atctacatag atggaaaagt caaagaagtc
     2341 agtgatatag agacgatcgt tttgtggatt ttgaaagaca ttaattccct caacaattac
     2401 aaaatcagca gctttgacac tttgtttctt ttcgggtacg atgtcgtaaa cttcatgaga
     2461 atagacagga atatctacat cttgtccatt tttgatgcgg tccaagaagt tgagaagagc
     2521 ttccatatca tagctttcag gaaatccttt acgatttaaa atcccctgct caatcaaggt
     2581 ttgattggga tagagaaaac catcagttgt aaccaactca accgtagcat ctgtaaacgt
     2641 acgggacagt aggatttgaa gtaggcgact ggttgtggat tttccaacgg caacactccc
     2701 agaaacccca ataataaaag gttgagattt actttcacgt tggaggaaaa ttccttttga
     2761 aaaggctaaa tcttccttag ttcgcttgta aatctgaatc agatgagcca aggggagata
     2821 gatatctgta acgtcttgga gactgatttg gtcattaaaa ctcttgatag attccaattc
     2881 ttcttctgtc aaaggaggtg ttgtctttcg atgtaaagat tgccaagtct ggcggctgat
     2941 tttttcaaaa tgtaaaaatt cgttggtcat ttgtttttcc ctagatattt tgtctatcat
     3001 accataaaaa gctaaaaaaa taaagcggtt tcttgaatgt ggtaagtcaa tggtatatag
     3061 tataagtgat caatttattg atgaaatagg agatagattc tactgattta taagcttata
     3121 aagggggctt tttagttcac aacttatagg aggtgtacta tgaaaatctt aaaacgttac
     3181 atattggaac tctgttttat tttaagtttt gctttacctt ttacaaaagg agccaatgca
     3241 gataatggta gatgctttgt ggaaacctat tacggtttta cttttttgat ggaacatgct
     3301 attgtaacag ctgtctttat ctgttcgatc ttaattgctt tcttactaaa aaaacgatgg
     3361 gcgaaatgga ttgctgctgg tagttattgc tttttagtcc tatggattgc gacagagggt
     3421 tatttcttcc gcatgtcact agaagatttg atacgacttt ggacaagttt ggaaatcctg
     3481 acacaaacgt atcagttggg cttttatcta aacattttgt tgggaatttt gttgataata
     3541 aagtatttta aggttaagca atagaaataa taatc
//
LOCUS       DAVMGZ010000147         3553 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000147 DAVMGZ010000000
VERSION     DAVMGZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.148"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..689)
                     /locus_tag="VYQ93_001852"
     CDS             complement(<1..689)
                     /locus_tag="VYQ93_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV3336491.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNSRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYVEIVKL
                     LLASEGKQTGITIIEKPIIRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDACLY
                     GDGYDFQVDIQEHSYLAEVKGIRKPKGRVRLTAYEFEKAKEFQSDFILSLVTNLDDIP
                     KLVLVDNPLKH"
     gene            complement(759..3410)
                     /locus_tag="VYQ93_001853"
     CDS             complement(759..3410)
                     /locus_tag="VYQ93_001853"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV3336492.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAINPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPELGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(3422..>3553)
                     /locus_tag="VYQ93_001854"
     CDS             complement(3422..>3553)
                     /locus_tag="VYQ93_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV3336493.1"
                     /translation="SKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN"
BASE COUNT          985 a          795 c          727 g         1046 t
ORIGIN      
        1 aaatgtttta gaggattatc aactaatact aactttggaa tatcatctag gttggtgacc
       61 agagataaaa taaaatctga ttgaaactct ttagctttct caaattcgta ggcagttaaa
      121 cggacacgac ccttaggttt tcgaatccct ttaacttcgg caaggtagga atgttcttga
      181 atatctactt ggaaatcata tccatcacca taaagacatg catcggttaa ctgtccacct
      241 tgaaattttt cttctttatc aaaatgaagg ataaaataat tctctgcctc catccccgtt
      301 tcctgtagac gtttgaattt agttcgaata attggttttt caattatagt aatgcctgtt
      361 tgctttcctt cactagcaag taacagttta acaatttcaa cataactatg aacatcttca
      421 tttccaaaca tcatatcaat taaatcttta cgaaaacggt aaacttcagc tttctgccac
      481 caaccttttc gactattgtc aaaataagga tcaaataaat ccattctatt ttttacaact
      541 ccagttgttt cagcaattcc cagagaaacc acatagcgat aaaactccga cttgctagag
      601 cattgaaatt cttttataaa aagcttgtca aacttggcaa gaccgtagcc aattaaattt
      661 aacaattcat aatgagggtg tttggacatc actttctcca tcaatagata ttgatttcat
      721 tataccaaaa aacacggctc atcaccgtgt ttctgtgttt atttcaccaa cttcttcatc
      781 tcatcaatac gtgctacggt cacatcatac ttggcttggt agtcggcttg tttgtctttt
      841 tctttttgga caacttctgg tttagcgttg gcgacgaagc gttcgttgct gagttttttg
      901 ccaaccatgt cgagttcttt ctgccatttg gcaagttctt tttccagacg tgccaattct
      961 tcttcgacat tcaagaggtc taccagtggc aagaagattt cggctcctgt aataacgctt
     1021 gacatagcca gttcaggtgc agggatggtt gatgcgattt ccaagtgttc tggatttgta
     1081 aagcgtttga tatagttgac attgctgtta aagaaggctt ccaagtcgct atcgcttgtc
     1141 ttaacaagga tggtgatagg cttgcttggt gctacattta cttccgcacg cgcattccga
     1201 acagcacgaa tcaagtcttt gagactttcc acaccagtgt gagccgcaag gtcttcaaag
     1261 gctagattaa cagttgggta tgcagctgtc acgatagaac cttctgagat ttgtccaaag
     1321 atttcctctg tcacgaatgg catgattggg tgaaggagac gaaggatctt gtccagcgta
     1381 tagaggagaa cagatcgagt aatgacctta tcgtcttcat tgtcgctgta tagaacttcc
     1441 ttggtcaact caacatacca gttggcaaat tcttcccaga tgaagttgta aaggatatga
     1501 ccagccacac caaactcgaa cttatcaaag ttttcagtaa cttttgcaat ggtttcgttg
     1561 agattgtgga gaatccagcg gtccgtcaca ttaccagcct cacctgttgc aacttttgtg
     1621 acattgtcat gcgccacatc cagcgtcaaa ccttcattgt tcatgaggat atagcgagaa
     1681 atgttccaaa ttttgttaat aaagttccat gaagcatcca ttttctcgta agagaaacga
     1741 acgtcttgac ctggtgcgga accgtttgaa aggaaccaac gaagggcatc agcaccgtat
     1801 ttctcgatga catccattgg gtcaatcccg ttaccgagag atttagacat cttgcgtcct
     1861 tgctcgtcac ggatgagacc gtggataagc acgttttgga atggctgacg accagtaaat
     1921 tccaaggact ggaagatcat acgagatacc cagaagaaga tgatgtcgta acctgttacc
     1981 aaggttgaag ttgggaaata acgtttaaag tcttctgagt cgacttcagg ccagcccatg
     2041 gttgaaaatg gccagagggc agaactgaac caagtatcca agacgtcttc gtcctgagtc
     2101 catccgtcac cttctggagc ttcttcgccg acatacattt caccatcagc attgtaccag
     2161 gcagggattt ggtgacccca ccaaagctga cgagagataa cccagtcgtg gacattttcc
     2221 atccattgaa ggaaggtatc gttgaaacga ggtgggtaga attcgacctt gtcctctgtg
     2281 tcttggttag caatggcgtt cttagccaat tggtccatct tgacgaacca ttgagtagac
     2341 aagcgtggct caactacgac acctgtacgt tctgagtgac caacactgtg gacacgtttt
     2401 tcgattttga caagggcacc gatttcttcc aacttagcaa cgactgcctt acgagcttca
     2461 aaacgatcca tgcctgaaaa ttcaaaggca agctcattca tagttccgtc gtcgttcatg
     2521 acgttgactt gtggcaagtt atgacgttgg ccaaccaaga agtcatttgg atcgtgggca
     2581 ggtgtgattt tcacgacacc agtaccaagc tcaggatctg cgtgctcatc tccaacgatt
     2641 gggatgagtt tattagcgat tggaaggatg acgtttttac caatcaagtc cttgtagcgc
     2701 gggtcttctg gattaatcgc aaccgcaacg tccccaaaca tagtctcagg acgagttgta
     2761 gcaacttcaa gggcgcgtga accatcttcc agcatgtaat tcatgtggta gaaggcacct
     2821 tctacatcct tgtgaatcac ctcaatatca gaaagggctg tgcgagctgc tgggtcccag
     2881 ttgatgataa actcaccacg atagatccag cctttcttgt aaaggttcac aaagacctta
     2941 cgaacagctt ttgacaaacc ttcatcaaga gtgaaacgct cacgagaata gtctacagaa
     3001 agccccatct tgccccattg ttccttgatg gtagtggcat attcgtcttt ccattcccag
     3061 accttcgtca agaaagactc acgacctagg tcataacgcg taataccctc accacgtaag
     3121 cgctcctcaa ccttagcctg agtcgcaata ccagcgtggt ccatacctgg aagccaaagg
     3181 gtatcaaagc cttgcatgcg tttttgacgg atgatgatat cctgcaaagt cgtatcccaa
     3241 gcgtgaccaa ggtgaagttt cccagttacg tttggtggtg gaatcacgat tgaataaggc
     3301 ttagcctttt gatcgcctga aggcttgaaa acatccgcat caagccattt ttggtaacga
     3361 ccagcctcaa cctcggctgg attgtattta gatgaaagtt ctttagacat gtgtgttctc
     3421 cttaattact tttattgaca attttaacca aaccaatttt tgaaagttca atataaatct
     3481 cttcaatttc agcaaatgct gcaagtggtg acatagtata atttccagga ttatatttaa
     3541 gatcatactt gct
//
LOCUS       DAVMGZ010000148         3546 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000148 DAVMGZ010000000
VERSION     DAVMGZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.149"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(47..1462)
                     /locus_tag="VYQ93_001855"
     CDS             complement(47..1462)
                     /locus_tag="VYQ93_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV3336494.1"
                     /translation="MTIFPDDFLWGGAVAASQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLLGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     EYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1475..3313)
                     /locus_tag="VYQ93_001856"
     CDS             complement(1475..3313)
                     /locus_tag="VYQ93_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV3336495.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEVGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3331..>3546)
                     /locus_tag="VYQ93_001857"
     CDS             complement(3331..>3546)
                     /locus_tag="VYQ93_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV3336496.1"
                     /translation="KLFAHRLVENTTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQ
                     TEYNYLLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1186 a          730 c          477 g         1153 t
ORIGIN      
        1 gagatgttgc cttaaccgtg ataatatcta atcatcaata aacaaactat ttgaataagg
       61 attctccatt tgattcaatc actcctttat accaagtaaa ggacattttc ttatatcgat
      121 tgaatgtgcc atttccatca tcatttcgat caacataaat gagaccgtac ctcttagaaa
      181 gctgtgcagt ggacatagaa acgcagtcaa tacatcccca agacgtatag cccataattt
      241 ccacaccatc ttgtagagct tcagctactt gcaataaatg ttctttcata tactgaattc
      301 tataatcatc ttggactgtt aagttattaa attcatcttt tattagttga tctttagcac
      361 ctaatccgtt ttccactata aataatggaa tttgataacg gtcataatat ctatttaaaa
      421 ttatacgtag tccaattgga tcaatttgcc atccccactc agaagactct aaataaggat
      481 ttactaagcc accaataata ttcccttctc ctgaattata ctgtgttgga agagcagact
      541 gagtcacact catgtaatag ctaaaggata aaaaatctac ggtataattt tttaataact
      601 ctgcatcttc agctgcaaac tctatgttaa tgtcatgttc cttaaaatat ctttttgcat
      661 aattcggata ataacctcta acatgcacat ctgaaaatag ataatttaga ttctcatact
      721 catgagtcgc ccatacatct tttggatttg gagtcattgg ataagctggc atagctaata
      781 ccatacaccc caccttaaac tctgaattaa tctcacgagc aatttttgta accaaactcg
      841 aggcgactaa ttcatgatgt atagcttgat ataattcttg tttcgaaaga ttctccttag
      901 gtatatctat tcctccacta gtaaatggta attccaaaac agagtttact tcgttaaatg
      961 taagccaata tttaacttta tctttatacc tttctaaaac tgttcgagca aatttttcat
     1021 aaaaatgaat cattctccta tcaacccatc catgatattt tcttgctaaa tataatggag
     1081 tctcatagtg tgaaagagtt acaagtggct ctatcccgtg agcatgtagt tcatcaaaca
     1141 attcatcata atattccaaa ccagcttcgt taggttcttc ttcatctcct tttggaaaaa
     1201 ttctactcca tgcaatagaa gtacgaaaaa cattaaagcc catttcagaa aacaaggata
     1261 tatcttcctt atatttatga taaaaatcaa tacctaacaa ttttaagtta tcttctgtag
     1321 gattttctgt tgcttctcct aatccacctt tgggtaacac atcctgaact gataatccct
     1381 taccatcttc attatatgct ccctctactt gactagctgc aacagctcca ccccaaagaa
     1441 aatcatctgg aaaaatggtc ataactttcc tccattataa tattaccagt aattccttag
     1501 aatgctcgat tgtctgatta ttaggtaata ctaatacatc tagaaaatca ttggtattcg
     1561 ttacaattac tggtgtaact gtttcgtagc ctttagtctt gattaaattc aagtccattt
     1621 caaaaatcaa ctgatttttg aaaactctgt ctccttcttc tacatgacta ataaaacctt
     1681 gaccttttag ctcaacagta tctaatccaa tatgaattag taactcaaca ccctcatcac
     1741 tcttcaatcc aattgcgtgc ttagtcggaa aaatatttgt aattttccca tcaaatggtg
     1801 cataaacctt accttcactt gggataatcg ctactccgtc tccaattagt ttatctgaaa
     1861 atgttttatc ctggacatcg cttaacggaa tgatttctcc tgatatagga gaaaatatca
     1921 tttttttatt tgaaactcca acttcaactt ctaaattgct agaactctct tcttcatcga
     1981 ttccaaatat ataagctaat acaaaggtaa taacaaccga aatgaccgcc acaattaaag
     2041 catttacaat atttgatggc acatcagaat aaataaattg aggcaacgct atcaaagatg
     2101 ggacagcaaa tagatacgct ttaacactag taagacctgc aaataatccc gctaatccac
     2161 caccaatcat agctgcataa agcggttttt tatattttaa agtcacacca tataatgcag
     2221 gttcggtaat ccctgcaagt aaggctgaga aacctgctgc aaaagcaatt tgttttgtat
     2281 tattattttt actctttaat gcaacagcca tcgaagcagc cccttgagct aagtttgacc
     2341 ctaacattgc tggaagaatt aatacgtctg gagtagcaat agatgccgcc aaaaaaatag
     2401 gtgcaaaagc ccaatgcatt ccagtcataa caataaatgg cataatagca ccaagaatag
     2461 ctaatgtaag ccatccagct acaccataca tttgcccaac tagatttgat aatccttcac
     2521 caacaattac tccaataggc ccgactacaa ctaaggcaat acagcttgat actaataata
     2581 ctagcgtagg ttgcaaaaaa ctcttagtaa cagctggtgt taatttagca atcatttttt
     2641 caatatattt catcaaccaa accataataa gaattggaac gactgacgaa ccataactag
     2701 ctggtgtcac aggtacacca aataaactaa gaggattccc tgattgcacc atttgaacaa
     2761 aatttggatg gagaagtaca cctgctacag acatagctaa tgtagatgtt acttttaatt
     2821 tttgtgatgc agaataagct aataacagcg gtaagaaata atatggagca tccccaaaaa
     2881 atgtcaaaaa agcaatagtc tgagaatctg attgcaatat accaagcatt ggtaaaataa
     2941 ttaccaagac tttcagcata cctcccccta acattgctgg aatgattgga gtcatggaac
     3001 cagcgatata ctcaatgatt ctttctaaaa tattcccttt gtgcccttga acaactgaat
     3061 cggattcaaa attgccaagt ttaacgaatt ctttataata attagctaca tcattaccaa
     3121 gtataatttg atattgtcca ttctttttca taatacctat tacacctggt atcttcttca
     3181 catcatcatc attgactaaa ttttcatctt ttaattctaa tcttaaacgt gttacacaat
     3241 gggtaactct attgacattt ttttcacctc caattacatc gaggattttt tgtaccgtat
     3301 ctttataact catggtattc tcctattcta ttaatctaaa ttttttgtta agcgacgaat
     3361 atgagccatc aaataaacta attcactaga agtcagcaaa taattgtact ccgtttgtat
     3421 aaacattgcc acctgttcac cacattcata ttctctagga tatttatttt tcattaatgc
     3481 taacaagtct tcatcatcat cgtcacaata agttgtattt tcaaccaagc gatgggcaaa
     3541 taattt
//
LOCUS       DAVMGZ010000149         3325 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000149 DAVMGZ010000000
VERSION     DAVMGZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.150"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            570..2585
                     /locus_tag="VYQ93_001858"
     CDS             570..2585
                     /locus_tag="VYQ93_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV3336497.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDSNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            2587..3219
                     /locus_tag="VYQ93_001859"
     CDS             2587..3219
                     /locus_tag="VYQ93_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336498.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
BASE COUNT         1012 a          583 c          688 g         1042 t
ORIGIN      
        1 gtttttatgt ttagtaacac aattccttac caacagttta ttcagaagaa tagacaattg
       61 gagattcgag tgcaatcaca aaagaagtcc aatggtcttg atgttgggaa ggctgattga
      121 tttcgtatta ctttaaaatt caactattta gagaaaggta ttctgatgag agcttgtaac
      181 aataccagat agatataaga atggagatgt ttgataaaat gctttcatag ctaaccaact
      241 gttgaaaatt atgagttaca aaatctaaag tatttaagat tggtttataa aattaggtct
      301 ctgcctgtag aaaggaacgt gcagttagaa aggaggaaaa aataggatac tataattcag
      361 actgagattt ctaattaaaa tatgtagttg catttcgctc attttcataa tatttcattc
      421 agaggagtaa taatatgtta aaatttagat ttgatgcggg atggtaaaga ataattgaag
      481 aagattcttt tatcaatagt ttagtaagct tgttagttta tagagggcag gggtatttct
      541 gccctctgtt ttagaataag gaatgcacta tgaaaaaatt atttatactc atatccaacc
      601 tgcttgctag tttgttcttt gtttgggtat ttaccatttg gactgataca tatgtctccc
      661 attattaccc caacgttgtt gtacgtgatt cttcccctga aacaactttt cagcatgttg
      721 ctacacgctt ggaaaaacta gcagaagaaa cagatagctt tattgcgatt caacaccaag
      781 attctaactc agaaggtact acagtttttt cttatacgac ctttggggac ggaaagttgc
      841 ctgatgggct tcaggaaaaa aaactagaag atgctcaaag tagtagtgtt gaaacaaact
      901 attttgtatt cgatggacac ctagatattc acttgctaag ggaggagcta agtcaacttg
      961 gcttgactaa catgaacctg acaattccat ctaaactatc tacgttaatg gctatcttta
     1021 gtaacggatt tcagttaatc agtctattga ttttcatctt aacctttgta gcactgactt
     1081 taattagtca aattagccaa ttacggtcgt cgggcattcg cctcatttca ggagagaaac
     1141 ggtggtccat tttccttaga ccagttggtg aggatttgaa agggatagct gttggtttca
     1201 gtttagctgg cgtgctcgcc attcttatgc aaaaaattct gtcgcttcca acgcaatccc
     1261 taatgaccat aggagcaggc ttgctcagtt ataatctcat cttgttgtcg atctccctgt
     1321 ttttcgctca actcttcgca gttgggataa aaaagataca cctgatgcag attataaaag
     1381 ggcaagtgcc tgtcagagga atcattagtt tgattttaat cggtcaacta ttagcgatta
     1441 ttattgtaac gctgggaata gggagtagct taaagtattc ccaagcctgg caacagcatc
     1501 ggattggaca agaggcttgg agtcaggaaa ggcaactgat taccctatca atcagccgtg
     1561 aaggaacgag tcctggtttt gatgaacaag ctcaaaggaa actcagaact tggtatcaat
     1621 tgatggatct ggctgtttca gaacaaaagg ctttcttatc tagacaccag ttaattgacc
     1681 gtactttgca aaatggcatg gcttcctcca aaaacttgat aacctctaca gaatggcacg
     1741 attacaaccc gaatggcaat gtccttatcg tcacaccgca atacttggag cgtcaaaaca
     1801 ttcctgtaga tacaactatt gagcaaaaga tgaatcacct taatgtaggg gagtttgtct
     1861 tattgctgcc tgaacacctc cgttcagagg aagaacatta taaatctgtt tttgaagacg
     1921 acttaaccag tcgcatgtct agtcaagatg aacgacagca aatgactgct acggtaggtt
     1981 atttagaatc aggtcaggat cgttttgtgt ataatacgac ccctatttct taccagcagt
     2041 ttttgaaaga tccaatcatc attgttataa caccccaatc aactggtcca cagtccattt
     2101 tgttttggat agacgcagta cagaactacg ttctctttaa tcaattgtct gatgcccagg
     2161 agcttatcca gagacaaggc attgaaaatt gggtctcaga aatgcaaaca ggttaccaca
     2221 actacatcac attattggat aatatccaga gggaacgttg ggtaatgcta gcaggagctg
     2281 tgcttgggat tgcaacttca atcttgttgt ttaacactat gaataggctc tactttgaag
     2341 aatttagacg tgccattttt atcaaacgca ttgcaggtct caggttctta gaaatccatc
     2401 gcacttatct ctttgctcaa ctgggtgtgt ttttactggg atttgttgcg agtgtatttc
     2461 ttcaggtaga gataggagtt gctttcttag tcttgttact ctttactggt ctatctcttt
     2521 tacagttaca tgtccaaatg cagaaagaaa acaagatgtc cattcttgtt ttgaagggag
     2581 gttaatatga ttgaacttaa acaggtgagt aaatcttttg gagaacgaga gttattttcg
     2641 aatctttcaa tgacatttga ggctggaaaa gtctatgcct taattggttc aagtggtagc
     2701 ggaaaaacaa ccttgatgaa catgattggg aaattagaac cttatgatgg gacgattttt
     2761 taccgaggta aagacttggc caattataaa tcgagtgatt ttttccgtca cgaattgggc
     2821 tacctcttcc agaactttgg cttaattgaa aaccaaagta ttgaagaaaa ccttaagcta
     2881 ggtctcattg gtcaaaagtt gagtcggtcg gaacagcggt tgaggcagaa gcaggcttta
     2941 gaacaggtcg gcctggctta tcttgaccta gataagcgca tctttgagtt atcgggcgga
     3001 gaatcgcaac gggttgcctt ggcaaaaatt atcttaaaga atccaccctt tattctggca
     3061 gatgagccaa cagcttcaat agacccagca acctctcagt tgattatgga gattttgcta
     3121 tctcttcgag atgataatag gctaatcatt atcgcaacac ataatccggc aatttgggag
     3181 atggctgatg aagtgttcac gatggatcat ctgaaataaa aatccttgtt tttaattgca
     3241 cgatgagtta ctgaaatatt atcatgaatc aagaattgga gttaatttag aattgtactt
     3301 tattaatatt gaggtaactt tttct
//
LOCUS       DAVMGZ010000150         3316 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000150 DAVMGZ010000000
VERSION     DAVMGZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.151"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1656
                     /locus_tag="VYQ93_001860"
     CDS             <1..1656
                     /locus_tag="VYQ93_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV3336499.1"
                     /translation="FFEKLSRNIYLRAIKDGFISSMPAVLFSSIFILIAAVPNIFGFK
                     WSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTNQINYMSTFLAAVV
                     GLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNNVTIRLPEEVPPNI
                     AQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFSATDGYIGLAIVFG
                     AYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKILNPVTQTFVVTMGG
                     TGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPIVLNPIFFIPFVTA
                     PIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSFVLAITLIIIDTLI
                     YYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKAGVDAAQNTITEET
                     NVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPEFDLVILAPQVAS
                     NFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            1871..3277
                     /gene="lacG"
                     /locus_tag="VYQ93_001861"
     CDS             1871..3277
                     /gene="lacG"
                     /locus_tag="VYQ93_001861"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV3336500.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDAPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
BASE COUNT         1015 a          578 c          668 g         1055 t
ORIGIN      
        1 ttctttgaga aattatcgag aaatatatac ttaagagcga taaaagatgg ttttatttcc
       61 agtatgccag cagtattatt ctcaagtatt tttatactaa tagctgctgt gcccaatatt
      121 tttgggttta aatggtctga tgaacagtta gcttttattt tgaaacctta caactactca
      181 atggggattt tagctctctt agtagctggg acaactgcaa aatcgttaac agattcagtt
      241 aatacacgta gtatggagaa aaccaaccaa atcaactaca tgtcaacttt tttagcagct
      301 gttgttggat tgttaatact ggcagctgat ccaattgaag gtggatttgc taatggtctt
      361 ttaggtacta gaggattact tactgcattt ttggcagctt ttattacagt aaatatctat
      421 aaagtatgta ttaaaaataa tgtaaccatt agacttcctg aagaggttcc accaaatatt
      481 gcacaagttt ttaaagatgt tattccgttt gccttatctg ttctttcaat atatggattg
      541 gatttaattg ttagaaatat ttttggtaca aatgtagcag aatctgttgg taaaatatta
      601 gcccctctat tttcagctac tgatggttat atcggtttag ctattgtatt tggtgcttat
      661 gctttctttt ggtttgttgg aattcatggg ccatctgttg tagaaccttt aattgtagct
      721 atcagttatg caaatataga agctaatgtt cagcttgtgc aagctggtat gcacgcagat
      781 aaaattttaa atccagttac tcaaactttt gttgtaacaa tgggtggtac tggtgcaaca
      841 ctagtagttc catttatgtt tatgtggtta tgtaaatcaa aaagaaatag aattgtcgga
      901 cgtgcgtctg tagttcctac tttctttggc gtaaatgaac caatcctatt tggtgctcca
      961 attgttttaa atcctatctt ctttattccg tttgttactg ctcctattat caatgtttgg
     1021 attatgaaat tttttgtaga tgttctacaa atgaatagtt tcagtattat tttaccttgg
     1081 acaacacctg ctccaattgg tattgttatg ggaacggcgc tggctccatt gtcattcgtc
     1141 ttggctataa ctttaatcat tatcgacact ttaatctatt atccatttgt taaagtttat
     1201 gaccatcaaa ttttagaaga agaacggaaa ggaaactctt catctgaatt gaaagaaaaa
     1261 gttgctgcaa acttcaacac tgcaaaagcg gatgctattc ttgaaaaagc aggtgtcgat
     1321 gcagcacaaa ataccatcac tgaagaaaca aatgtcctcg ttctctgtgc tggtggaggt
     1381 acaagtggac tccttgcaaa tgctttgaat aaggcagcag cagaatacaa tgtccctgtg
     1441 aaagcagcag caggcggcta tggtgctcac cgtgaaatgt taccagagtt tgatttggtt
     1501 atccttgctc ctcaagttgc ttcaaacttt gaagatatga aggcagaaac agataagctc
     1561 ggtattaaac tagcgaaaac agaaggcgct caatacatca aattaactcg tgatggaaaa
     1621 ggtgccctag cattcgtaca agaacaattc gattaaggct aggaactgtg aaacagtctc
     1681 ctatcgttac ggaaatcgct atggcgaatt tcctaatcgc cttattaatt cgtcggtaaa
     1741 aagatatcgt ttttacctcc tcatgtcaca attcggtgac ttggtacaag aagtgagatg
     1801 gagaaggatg gctcactgac tcctctcctc tcacttttac tttatttaaa tcaagaaata
     1861 ggtgaaaaaa atgacaaaaa cacttccaaa agactttatt tttggtggcg caacagctgc
     1921 ctatcaagca gaaggtgcta cacatactga tggtaaagga ccagtagcgt gggataaata
     1981 tcttgaagat aactactggt acactgcaga accagccagt gatttttaca atcgatatcc
     2041 agttgatctc aagctagcag aagagtatgg tgtcaatggc attcgaattt ctattgcctg
     2101 gtcacgtatt ttcccgactg gttatggtca agtaaatgct aaaggtgttg agttttatca
     2161 taatttattt gcagagtgtc acaaacgtca tgttgagccc tttgtaactc ttcatcactt
     2221 tgacgcgcca gaagctctcc actcaaatgg agacttctta aaccgtgaaa atatcgaaca
     2281 ttttgtagac tacgctgcct tctgttttga agaatttcca gaagtaaact attggacaac
     2341 cttcaatgaa attggaccaa tcggtgatgg tcaatatttg gttgggaaat tccctccagg
     2401 tatccagtac gaccttgcca aagtctttca atcacaccac aatatgatgg tgtctcatgc
     2461 atgcgcggta aaattgtaca aagagaaagg ctataaaggg gaaattggtg ttgttcacgc
     2521 cctgcctact aaatatcctc tagatcctga aaatccagca gatgttcgtg cagcagagtt
     2581 ggaagatatc atccacaata aattcatctt agacgcaact tatctaggtc gctattcagc
     2641 tgaaaccatg gaaggtgtca accatatctt atcagtcaat ggtggtagtt tggatcttcg
     2701 tgaagaagat tttacagcat tagaagctgc aaaagacttg aatgatttcc taggaatcaa
     2761 ctactatatg agtgactgga tggaagcctt tgatggagaa actgaaatta tccataatgg
     2821 taaaggtgaa aaaggaagct ctaagtatca aatcaaaggt attggtcgtc gtgtagctcc
     2881 tgactatgta tcacgcacgg attgggattg gattatctac cctcaaggtt tgtatgacca
     2941 aatcatgcgt gtgaagaaag attatcctaa ctacaagaag atttacatca ctgaaaatgg
     3001 tctcggctat aaagatgagt tcgttgataa cactgtttac gatgatggtc gtattgatta
     3061 cgtgaaacaa cacttggaga tcttgtctga tgcgattgca gatggagcta atgtaaaagg
     3121 ttacttcatt tggtcattaa tggatgtctt ctcatggtca aacggttatg agaaacgtta
     3181 tggtctcttc tacgtagatt ttgaaactca agaacgttat cctaagaaat cagctcactg
     3241 gtacaagaaa gtagcggaaa ctcagattat agactagtag aattagtcat tagatataga
     3301 attttagtga gtcaaa
//
LOCUS       DAVMGZ010000151         3299 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000151 DAVMGZ010000000
VERSION     DAVMGZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.152"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            120..1631
                     /locus_tag="VYQ93_001862"
     CDS             120..1631
                     /locus_tag="VYQ93_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV3336501.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            1653..2984
                     /locus_tag="VYQ93_001863"
     CDS             1653..2984
                     /locus_tag="VYQ93_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV3336502.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICQDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLYGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAIYRLAT"
BASE COUNT          903 a          679 c          705 g         1012 t
ORIGIN      
        1 actagttttt tagactttaa tatggtaaaa caaatataga tggaaagcgt tttcttaaat
       61 gtttatttaa gaaagtagtt ggttttttac actttgttag acatcaggag gaaaaacaaa
      121 tgagtgaaaa agctaaaaaa gggtttaaga tgccttcatc ttacaccgta ttattgataa
      181 tcattgctat tatggcagtg ctaacttggt ttatccctgc gggggccttt atagaaggta
      241 tttacgagac tcagcctcaa aatccacaag ggatttggga tgtcctcatg gcaccgattc
      301 gggctatgct aggtactcat ccagaggaag gttcgctcat taaagaaacg agcgcagcga
      361 ttgatgtagc cttcttcatc cttatggttg gtggtttcct tggcattgtc aacaaaactg
      421 gtgctcttga cgtagggatt gcctctatcg tgaagaagta taagggccgc gaaaaaatgt
      481 taattttggt attgatgcct ttgtttgccc tcggtggtac aacttatggt atgggtgaag
      541 aaacaatggc cttctatcca ctccttgtgc cagttatgat ggccgttggt tttgatagcc
      601 tgactggtgt tgcaattatt ttgctcggtt ctcaaatcgg ctgtttggca tctactctga
      661 atccatttgc gacaggtatt gcttcagcga ctgcgggagt tggtacaggg gacggtatcg
      721 tacttcgtct gatcttctgg gttaccttga ctgctcttag tacttggttt gtttaccgtt
      781 atgcggataa gattcaaaaa gatccgacta agtcactggt ttatagtact cgcaaagaag
      841 atttgaaaca ctttaacgta gaagaatctt catctgtaga atctacactt agcagcaaac
      901 aaaaatcagt tctcttctta tttgtgttga cattcatctt gatggtattg agcttcattc
      961 catggacaga ccttggcgtt accatttttg atgactttaa tgcttggttg actggtcttc
     1021 cagttattgg taatattgtc ggttcatcta cttctgcact aggtacttgg tacttcccag
     1081 aaggcgccat gctctttgcc tttatgggta tcctgattgg tgttatttat ggtcttaaag
     1141 aagataagat tatctcttcc ttcatgaatg gtgctgctga cttgctcagt gttgccttga
     1201 tcgtagcgat tgctcgtggt attcaagtta tcatgaacga cggtatgatt accgatacaa
     1261 tcctcaactg gggtaaagaa ggcttgagcg gtctatcttc acaagtcttt atcgttgtaa
     1321 cttatatctt ctatctacct atgtcattct tgatcccatc ttcatctggt cttgccagcg
     1381 caactatggg tatcatggct ccacttggag aatttgtaaa tgtccgtcct agcttgatta
     1441 tcactgctta ccaatctgct tcaggtgtct tgaacttgat tgcaccaaca tctggtattg
     1501 tgatgggagc tcttgcactt ggacgtatca acattggtac ttggtggaaa ttcatgggca
     1561 aactcgtagt cgctattatt gtagtgacca tcgcccttct tctccttgga accttccttc
     1621 cattcctata aaatagtgag tgaggtgatt ccatgaaaat agatataaca aatcaagtta
     1681 aagatgaatt tcttatatca ttaaaaacct tgatttccta tccttcagta ctcaatgaag
     1741 gagaaaatgg aacacctttt ggacaagcaa tccaagatgt cctagaaaaa actttagaga
     1801 tttgtcaaga cataggtttc actacctatc ttgaccctaa aggttattac ggatatgcag
     1861 aaatcggtca gggagcagag cttctggcca ttctctgtca tttggatgtt gttccatcag
     1921 gtgatgaagc agattggcag acaccgccat ttgaagcaac tatcaaagac ggctgggtat
     1981 tcggacgtgg tgtccaagat gataaaggcc cttcgctcgc agctctctat gcagtaaaaa
     2041 gcttgctgga ccaaggtatt cagttcaaaa agcgcgtacg ctttatcttt ggtaccgatg
     2101 aggaaaccct ctggcgctgc atggcacgct acaataccat cgaagaacag gccagcatgg
     2161 gctttgcacc tgactcatct tttcctctga cctatgctga aaaagggctt ctacaggtca
     2221 aactttatgg ccctggatcg gatcaactag agcttgaagt aggaggcgcc tttaacgttg
     2281 taccagacaa ggccaactac caaggtcccc tctatgaaca ggtttgtaac ggtctcaaag
     2341 aagctggtta tgattaccaa accactgaac aaaccgtaac ggttctcgga gtgccaaagc
     2401 atgctaagga tgctagtcaa ggtatcaatg ctgtcatccg actagctacc attcttgctc
     2461 ctctccaaga acaccctgct ctcagttttc ttgcaacaca agcaggtcaa gacggcacag
     2521 gaagacaaat ctttggtgat atagcagatg aaccttctgg tcacctatcc tttaatgtcg
     2581 caggtctcat gatcaatcat gaacgttctg aaatccgtat tgacattcgg actcctgtct
     2641 tagctgacaa ggaagaacta gtagagttgc ttacaagatg tgcacaaaac taccaactcc
     2701 gctacgaaga gtttgactat ctagcgcctc tatacgtcgc aaaagacagt aaactcgtta
     2761 gcacactgat gcaaatctac caagaaaaga ctggcgataa cagtcctgct atttcatccg
     2821 gtggtgccac ttttgctcgc accatgccaa attgtgtagc cttcggcgcc ttattcccag
     2881 gagcgaagca gacagaacat caggcaaatg aatgtgccgt tctagaagat ttgtaccgtg
     2941 ctatggatat ttatgccgaa gccatctatc gacttgcaac ttaatcaggc aactgtttct
     3001 acaaaaaaaa atcgaccgat taatgaactg caccccaaaa gttagacaga ataaatctaa
     3061 cttttggggt gttttattat gaaattgaat tatgaagata aagttcagat ctatgaacta
     3121 agaaagcaag gacaaagctt caaacagctt tcaaaaagat ttggtgtgga tgtttctggt
     3181 ctaaagtcat ctgaatcttt gagatgagct ttataaatcg cttttttcag tttttgcact
     3241 ggtgtttcga taaactcaaa ctttttagcc gtggtattgc ctgattttat agtatattg
//
LOCUS       DAVMGZ010000152         3196 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000152 DAVMGZ010000000
VERSION     DAVMGZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.153"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            22..3141
                     /gene="phtE"
                     /locus_tag="VYQ93_001864"
     CDS             22..3141
                     /gene="phtE"
                     /locus_tag="VYQ93_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV3336503.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSISKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNKKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1187 a          532 c          626 g          851 t
ORIGIN      
        1 atttattgaa atgagggatt tatgaaattt agtaaaaaat atatagcagc tggatcagct
       61 gttatcgtat ccttgagtct atgtgcctat gcactaaacc agcatcgttc gcaggaaaat
      121 aaggacaata atcgtgtctc ttatgtggat ggcagccagt caagtcagaa aagtgaaaac
      181 ttgacaccag accaggttag ccagaaagaa ggaattcagg ctgagcaaat tgtaatcaaa
      241 attacagatc agggctatgt aacgtcacac ggtgaccact atcattacta taatgggaaa
      301 gttccttatg atgccctctt tagtgaagaa ctcttgatga aggatccaaa ctatcaactt
      361 aaagacgctg atattgtcaa tgaagtcaag ggtggttata tcatcaaggt cgatggaaaa
      421 tattatgtct acctgaaaga tgcagctcat gctgataatg ttcgaactaa agatgaaatc
      481 aatcgtcaaa aacaagaaca tgtcaaagat aatgagaagg ttaactctaa tgttgctgta
      541 gcaaggtctc agggacgata tacgacaaat gatggttatg tctttaatcc agctgatatt
      601 atcgaagata cgggtaatgc ttatatcgtt cctcatggag gtcactatca ctacattccc
      661 aaaagcgatt tatctgctag tgaattagca gcagctaaag cacatctggc tggaaaaaat
      721 atgcaaccga gtcagttaag ctattcttca acagctagtg acaataacac gcaatctgta
      781 gcaaaaggat caattagcaa gccagcaaat aaatctgaaa atctccagag tcttttgaag
      841 gaactctatg attcacctag cgcccaacgt tacagtgaat cagatggcct ggtctttgac
      901 cctgctaaga ttatcagtcg tacaccaaat ggagttgcga ttccgcatgg cgaccattac
      961 cactttattc cttacagcaa gctttctgcc ttagaagaaa agattgccag aatggtgcct
     1021 atcagtggaa ctggttctac agtttctaca aatgcaaaac ctaatgaagt agtgtctagt
     1081 ctaggcagtc tttcaagcaa tccttcttct ttaacgacaa gtaaggagct ctcttcagca
     1141 tctgatggtt atatttttaa tccaaaagat atcgttgaag aaacggctac agcttatatt
     1201 gtaagacatg gtgatcattt ccattacatt ccaaaatcaa atcaaattgg gcaaccgact
     1261 cttccaaaca atagtctagc aacaccttct ccatctcttc caatcaatcc aggaacttca
     1321 catgagaaac atgaagaaga tggatacgga tttgatgcta atcgtattat cgctgaagat
     1381 gaatcaggtt ttgtcatgag tcacggagac cacaatcatt atttcttcaa gaaggacttg
     1441 acagaagagc aaattaaggc tgcgcaaaaa catttagagg aagttaaaac tagtcataat
     1501 ggattagatt ctttgtcatc tcatgaacag gattatccaa gtaatgccaa agaaatgaaa
     1561 gatttagata aaaaaatcga agaaaaaatt gctggcatta tgaaacaata tggtgtcaaa
     1621 cgtgaaagta ttgtcgtgaa taaagaaaaa aatgcgatta tttatccgca tggagatcac
     1681 catcatgcag atccgattga tgaacataaa ccggttggaa ttggtcattc tcacagtaac
     1741 tatgaactgt ttaaacccga agaaggagtt gctaaaaaag aagggaataa agtttatact
     1801 ggagaagaat taacgaatgt tgttaatttg ttaaaaaata gtacgtttaa taatcaaaac
     1861 tttactctag ccaatggtca aaaacgcgtt tcttttagtt ttccgcctga attggagaaa
     1921 aaattaggta tcaatatgct agtaaaatta ataacaccag atggaaaagt attggagaaa
     1981 gtatctggta aagtatttgg agaaggagta gggaatattg caaactttga attagatcaa
     2041 ccttatttac caggacaaac atttaagtat actatcgctt caaaagatta tccagaagta
     2101 agttatgatg gtacatttac agttccaacc tctttagctt acaaaatggc cagtcaaacg
     2161 attttctatc ctttccatgc aggggatact tatttaagag tgaaccctca atttgcagtg
     2221 cctaaaggaa ctgatgcttt agtcagagtg tttgatgaat ttcatggaaa tgcttattta
     2281 gaaaataact ataaagttgg tgaaatcaaa ttaccgattc cgaaattaaa ccaaggaaca
     2341 accagaacgg ccggaaataa aattcctgta accttcatgg caaatgctta tttggacaat
     2401 caatcgactt atattgtgga agtacctatc ttggaaaaag aaaatcaaac tgataaacca
     2461 agtattctac cacaatttaa aaggaataaa gcacaagaaa actcaaaact tgatgaaaag
     2521 gtagaagaac caaagactag tgagaaggta gaaaaagaaa aactttctga aactgggaat
     2581 agtactagta attcaacgtt agaagaagtt cctacagtgg atcctgtaca agaaaaagta
     2641 gcaaaatttg ctgaaagtta tgggatgaag ctagaaaatg tcttgtttaa tatggacgga
     2701 acaattgaat tatatttacc atcaggagaa gtcattaaaa agaatatggc agattttaca
     2761 ggagaagcac ctcaaggaaa tggtgaaaat aaaccatctg aaaatggaaa agtatctact
     2821 ggaacagttg agaaccaacc aacagaaaat aaaccagcag attctttacc agaggcacca
     2881 aacaaaaaac ctgtaaaacc agaaaactca acggataatg gaatgttgaa tccagaaggg
     2941 aatgtgggga gtgaccctat gttagatcca gcattagagg aagctccagc agtagatcct
     3001 gtacaagaaa aattagaaaa atttacagct agttacggat taggcttaga tagtgttata
     3061 ttcaatatgg atggaacgat tgaattaaga ttgccaagtg gagaagtgat aaaaaagaat
     3121 ttatctgatc tcatagcgta aggaatagca gtagaaaaag tctgaatcaa aaatgaagtt
     3181 ctctcaaaag ttagaa
//
LOCUS       DAVMGZ010000153         3194 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000153 DAVMGZ010000000
VERSION     DAVMGZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.154"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..232
                     /locus_tag="VYQ93_001865"
     CDS             <1..232
                     /locus_tag="VYQ93_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336504.1"
                     /translation="AVSQEELEKLEKSYDEKSHEELVSIESNLEMKQQNLIDEVNKTI
                     KENDANIQYISSSRRGEFVEKIIGRVVEKYGH"
     gene            222..2213
                     /locus_tag="VYQ93_001866"
     CDS             222..2213
                     /locus_tag="VYQ93_001866"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEV3336505.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYNSLLENNIEVQEIFSNDREYGVVVFY
                     QSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKYLID
                     LQWQIDYILSIYARQISKNNFLCTPHLVALEGWIEETRILYFIKVMDEHFGHSIYIYE
                     SETLTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            2229..2705
                     /locus_tag="VYQ93_001867"
     CDS             2229..2705
                     /locus_tag="VYQ93_001867"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEV3336506.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            2739..>3194
                     /locus_tag="VYQ93_001868"
     CDS             2739..>3194
                     /locus_tag="VYQ93_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336507.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNYLFSEQPI"
BASE COUNT         1100 a          420 c          616 g         1058 t
ORIGIN      
        1 agctgtttcc caagaagaat tggaaaaact agaaaaaagt tatgatgaaa aaagtcacga
       61 agaattagtt tcaatagaaa gcaatttaga aatgaaacaa cagaacctta ttgatgaggt
      121 taataaaaca atcaaggaaa atgatgcaaa tattcagtat atttcatcaa gtaggagagg
      181 agaatttgta gaaaaaatta ttggtagggt ggtagaaaaa tatggccatt agtcagatga
      241 aaagaatctc tctactattt tctaaaagta gtcttgatga tgttttaaaa actattcaag
      301 aactagagtc agtgcagttc cgtgatttaa aggttcagga taactggtca gaagctctag
      361 aaaaagatga agttgtattt ccaactattc aaatttttca tacttctaat tccaatcatg
      421 gggttattga gggaaatgat gccttgactt atttgatgaa tcaacaacaa catttagaag
      481 caactgtaga gaaattacaa gaatacctac cgaaagaaaa cacgtttaaa ttattgcagc
      541 aacctccgat aactacctct tatgaagaat tagagaaatt tggtaaagct aatgttgctg
      601 agggtgttct taaaaaagtg aatcatcaaa ttaacagagt tcatgaatta gaaagacaca
      661 ttcaaagtaa taatgaggaa atagagcgat taataaagtg ggaaaaatta gaaattgttc
      721 ctgcgaattt agaacaattt tctttctgta aaggaaaagt cggaacaatt ccaaggactg
      781 aagataatcg cttatacaat agtcttttag aaaacaatat tgaagttcaa gaaatatttt
      841 ctaatgatag agagtacggt gttgttgttt tctatcagtc tagttactct atagattttg
      901 atgaatactt atttgaacca tttgattatt ctagaaagga attaccgaag cagcgagtag
      961 tagatttaga tcaagaaaac atgcagttaa taactgaaaa agagaatatt atcgcatcgt
     1021 tgcaagattc aaagaaatat ttgatagatt tacaatggca aatagactat attttatcta
     1081 tctatgctcg tcaaatctct aagaataact ttttgtgcac tccgcatcta gttgcattag
     1141 aaggatggat agaagaaact cgtattttat attttataaa agttatggat gagcattttg
     1201 gacattctat ttatatttat gaatcggaaa cattgacgga taatcaagat gaaataccta
     1261 tcaaattaac gaatcattct ttaattgaac catttgaatt attgacagaa atgtatgctc
     1321 tgcccaaata ttatgagaaa gatcctacac ctgtattagc accattttac tttacatttt
     1381 ttggaatgat ggttgctgat ttaggctatg gtttactatt gtttttagga acaatgttag
     1441 cattaaaaat ttttcatcta ccttcagcaa ctaagagatt tttaaaattc tttaatatat
     1501 taggggtagc cgttgcaatt tggggtggaa tctatggctc attttttgga tatgagttgc
     1561 catttcatct gatatctaca acctctgatg tcatgactat attagtagtg tcagttgtgt
     1621 ttgggtttat tacagtattt gcaggtttgt tagcttcagg actacaaaaa gtaagaatga
     1681 agaaatatgc agaagcatat aattcaggat ttgcgtggtg tgttattctg cttggcttgt
     1741 tatttattgc tgttggaatg ttgatgcctg atatgagacc gttatttgta ttagggaaat
     1801 gggtatctat ttttaatgct gtggggattt tgattgtttc tattattcaa accaaaagct
     1861 tgtcaggtat tggagcagga ttgtttaatc tatataacat ttcatcttat ataggtgatt
     1921 tagttagttt cactcgattg atggcattag gattatctgg agcaagtata gcatcagctt
     1981 tcaatttaat tgttggtttg tttccgggaa tattggctaa actgacaatt ggattagtat
     2041 tattcattct tttacatgcg atcaatattt ttctatcgtt actatcagga tatgttcatg
     2101 gagcacgtct gatatttgtt gaattttttg gtaagtttta tgagggtgga ggaaaaccat
     2161 ttcaaccttt gaaggcttct gagaaatata ttaaggttat tacaaagaat taatggagga
     2221 tatatataat ggaacattta gcaacttatt tttcaaccta tggaggagct ttcttcgctg
     2281 cattgggaat tgtattggcg gttggattaa gcggtatggg gtctgcttat ggagttggta
     2341 aggctgggca atctgccgca gctttactga aagaacagcc tgaaaagttt gcctcagctt
     2401 tgatattgca attattgccc ggaacacaag gattatatgg ttttgttatt ggaattttaa
     2461 tttggttgca attaactcca gaacttcctt tagaaaaagg cgttgcttat ttctttgtag
     2521 ctcttccaat tgctattgta ggatactttt cagctaagca tcaaggaaat gtagcagtag
     2581 cgggaatgca aatcttggct aaaagaccaa aagaattcat gaagggagca attttagctg
     2641 ccatggtaga aacctatgca attcttgctt ttgtcgtatc attcattttg acccttcgtg
     2701 tataagaaat aaatttgcaa ttcaaaggag gtgtctaaat gagcaattta gaaaacttac
     2761 gagagtctgt tattgaacaa gctcatgaaa aagggcgtat gaaattattg gattccaaaa
     2821 agaagattga tgatgaattt gaaatgcaaa agtcgctcat tataaagaaa aaagaagctg
     2881 aacatgaacg aaagttaaaa gaattgcaac agaaatatca aataattttt caacaattaa
     2941 aaaataagga acgccaatca acgttagtat caaaacagaa aatattaaaa gaactttttc
     3001 aatctgcttt actagaaatg gaatcttgga gtgcagataa agaaatggag ttcatctatc
     3061 gaattctgga acgatattca caacaagagg tcatagtaac ctttggggaa cggactttag
     3121 ctaaattcaa tttggaacaa ttagagaaat tgaaattctc ttttccaaat tatttattta
     3181 gtgaacaacc tatc
//
LOCUS       DAVMGZ010000154         3187 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000154 DAVMGZ010000000
VERSION     DAVMGZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.155"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     regulatory      complement(138..226)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(435..1064)
                     /locus_tag="VYQ93_001869"
     CDS             complement(435..1064)
                     /locus_tag="VYQ93_001869"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV3336508.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     ESALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(1066..1848)
                     /gene="thiM"
                     /locus_tag="VYQ93_001870"
     CDS             complement(1066..1848)
                     /gene="thiM"
                     /locus_tag="VYQ93_001870"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV3336509.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGVSPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVVSKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            1939..2025
                     /locus_tag="VYQ93_001871"
                     /pseudo
     CDS             1939..2025
                     /locus_tag="VYQ93_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(2175..2843)
                     /locus_tag="VYQ93_001872"
     CDS             complement(2175..2843)
                     /locus_tag="VYQ93_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV3336510.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(3058..3146)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          989 a          685 c          454 g         1059 t
ORIGIN      
        1 ttttctaatt gatttgttcg catcttattt caatctacta tatcatcttc atccagtttc
       61 gtaaaagaaa aaactctaat tacagatacg aattagagtt cagcttacaa gattagacag
      121 ttcttttcga catacgaaaa aaacatttca catttccctt cgccagtctt aactgtatca
      181 ggttcaatgg gtatcatctc agcctaaagc accccaaatg tctttattat ttaattatgt
      241 gattattata acacacattt tatactagtt caagaaattg aactggaaat acagccttgc
      301 actcacaaag acagcagatc tttcttttgc aaaaaacaaa tgacctgttt gatgaattag
      361 ccattcaagc tgaatctaga catagctttt taaaaaagga aaatcctact tacttagaat
      421 ccaaggatag atatctattg ttcactcatt tcccgaacag ttttttctat attttttgca
      481 tacgatattg ccgaaatgat tgaaacgccg tcaacattgg tctttataat gtctttaata
      541 tgtttcgtct gtatcccacc aattgcaact aaaggcattt gtggcaatag ttttctcatc
      601 aattcaagac cttcataacc tatagcacca ccagcatcat cttttgactg ggtatcaaat
      661 acaggcccaa cacctacata atctacatat tcaacttttg attgttgaaa ttcttcttcg
      721 tttcttatag aaagaccaat tattttatct ggcatcaatt ttctaatttc atcaacacca
      781 atatcatctt gaccaacatg tacgccatcg gcgtcaattt ccattgctaa atctatatcg
      841 tcattaacga taaatggaac attgtatttt ttacaaagtt ctttaatttg gatagctagc
      901 tcaagttttt ctaagccttc taaagcactc tcaccttttt ctcgaaattg aaataaggtt
      961 ataccacctt ttaaggcttc ctcaacgact gtatatagat tttttccttg gcaagtagtc
     1021 gttccacaaa taaaatatag ttttagtaat tctttatgaa acatcttact tcactctttt
     1081 gaattccttt acatcttcat ctgtaatctc gtataaggca tttataaatt caactttaaa
     1141 tgtcccagga agatgtccat ttggacgttt ttctgccatt tctccagcga tattgtaaac
     1201 caacattgct gtttctaatg atttcaattc ttgacctttt tctagtccga taaagcttgc
     1261 tactacagct cctaataagc atcctgtccc aatgactttc ggcatcatag cactaccatt
     1321 atgaatcgtt accacttctc cattaacagc aatggcatcc acttcacctg ttactactat
     1381 tggaatattg aacttctcat ttgctgctag agcaatttcg tcaatattat ctacgcccgc
     1441 actatctact cctttagata ccacatctat tcctactaaa gaggcaatct cgccagcatt
     1501 tcctctaatc gctgctagtt tataattgtt gattagatca tctgctactt tttttctata
     1561 ttctcctgct ccacaggcta caggatctaa aactgctggg acattatatt tctccgcaat
     1621 tttcagagca gcttggtata atttccaatt ttcatctgtc aatgttccta tgtttattaa
     1681 taaaccacca gcatacttta acaaatcctc taaatctgct ggaaactcac tcatggctgg
     1741 tgagacgccc agtgctacta atccatttgc tgtgaaattt tttactacat cattggtgat
     1801 acaaatgacc aatggtgctt tttcttttaa taattttaaa cttgtcatat tgaaatcctt
     1861 ccttttcact ttatacgatc tactgatttc gatttatctc tatctttcgt taagaaattt
     1921 ttttcattta cgttgaatga tttatactca atgaaaatca aagagcaaac taggaagcta
     1981 accgcagatt gctcaaaaca ctgctttgaa cctgtagata agactgacga agtcagctca
     2041 aaacactgtt ttgaggttgt ggatagaact gacgaagtca gtaaccatac ctacggcaag
     2101 gtgaaactga cgtggtttga agagattttc gaagagtatt acctcatcaa atttgtaaat
     2161 atcatgagcc ttctctagac atcgtaacca atatcaaaaa aagctaattc taaagcgact
     2221 gcttgattcc agcgttgctg aagttctgtc aaatcttctc gatttttacc gacacgattg
     2281 agttcgtcaa ccagaaattg aacccactct gcaaagaaag gacctctgtg gagattgatc
     2341 cattccgaat gaatatagac ttcaggtaaa gccaaatctt tagaacccca gtctaaatag
     2401 agaccttctg caatgaccag catgaccaaa agatgggcat agtctgatga agccaccgcc
     2461 gaatacatta gatcctgaaa ggcttttgtt acagggtgca aagtcacttc tagatagtca
     2521 ttctctgcta cttttaactc tttaaaagcc ttttggaaat aaccatcttc atctgcttca
     2581 agaaagccta gttgcttggc aaaacgaagc ttggattcaa gtttatctgc gtgagctacg
     2641 caggcaccca gcatggataa gaaggcatca aagaagtgat aatcttgaat cagatagtcc
     2701 tttaagacct tattctcaat tgtccccgca aaaagttcct taacaaaacg atgattgatt
     2761 gcagcctgcc aatccttctg actgcttttt aataattctc caacagtcaa acctggctga
     2821 aatgcatagt cttgtgtttc catatttact tctcctctct ttacttgtta gtaattaata
     2881 aaacaccaag aaatatcaag caaaatcgta attccacttg atccttttaa aacacatcga
     2941 gagtatttgc agagagctaa ctaaacaagc ctatccagtt tatataaaca aaaaactcca
     3001 attacaatca agaattagag ttgacttaca agattagacc gttcatttca ccatacgaaa
     3061 aaactgttca catttccctt cgccagtctt aactgtatca ggttcaatgg gtattatctc
     3121 agcctaaagc accccaaatg tctctattat ttaattactg aaccagtata gcaaaaaatg
     3181 aaagcct
//
LOCUS       DAVMGZ010000155         3183 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000155 DAVMGZ010000000
VERSION     DAVMGZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.156"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            26..757
                     /locus_tag="VYQ93_001873"
     CDS             26..757
                     /locus_tag="VYQ93_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336511.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHASYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            772..1923
                     /locus_tag="VYQ93_001874"
     CDS             772..1923
                     /locus_tag="VYQ93_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV3336512.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDNYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(1975..2634)
                     /locus_tag="VYQ93_001875"
     CDS             complement(1975..2634)
                     /locus_tag="VYQ93_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV3336513.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFCCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLSKVILITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            2704..3150
                     /locus_tag="VYQ93_001876"
     CDS             2704..3150
                     /locus_tag="VYQ93_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3336514.1"
                     /translation="MTIRFEEKVSTENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRVQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
BASE COUNT         1162 a          473 c          657 g          891 t
ORIGIN      
        1 aaaagtattt ggaggaggaa gaattatgtt gaatcaggat ctctttgatt cgcttgaggc
       61 acaaaaaatt gtagatactt tgatgaaagg gcaaaaagat tatgtagatg aacgtctaga
      121 aaaaagagag acaatgatag tgtcgaatgg ttatgcatgg acgcgaccta atcatattga
      181 tactgcattt gcatcagcag atttgtttga gtataaatta caattagcag gacagacttg
      241 gggatattta gaatttgaaa caaatacaga aaaatatggg aaagtattgt taattataaa
      301 gggtaagaag cgacttacga accaatttcc tttggtacaa aaaaataaga gtggctactt
      361 atttgaatat gctcagatga atacacttta tcttaatcaa catgcttcct acaaaaatga
      421 tgaagatagt cattcctttc caattcagat ggagttagtt tctgatgaaa tgattcaaga
      481 aattgaacaa gctactaaaa attcgaatat cgaaaaattt atgattttaa cttatgaggc
      541 ggactcagaa aacaatatta tatctgtaga tgttgttatg cctgatgcac gaactggtca
      601 gttacacttg attcaagatt tgtctgagta tattcaatca agttcgtacc atttcgaaga
      661 agctaaatat caagatattc ctaatttttc agaattatcg gaaacagaag attttgaaat
      721 tattccaaga atagaaaaac aagaaggtca aaagtagtaa ggagtatgta aatgtttaat
      781 ggtcgggtat tgaaagaatt acggctgtta aatggtttaa gtagagcaga attagctcag
      841 agaattaatt taacggaaca agccatttgg cagtttgagt ccaacgaaac gaaacctaaa
      901 ttatcaacca aaatgcattt ggccaaccaa tttcatgttg atttaactta ttttgaacag
      961 gaagaagaga gcattcgatt tgattcttct gtaattgcct ttagaaatgc agacctagca
     1021 acacggaaaa caatagatat tcaaactatg tatttacata aggtagatag tttgattgat
     1081 tattttgaaa gttttgtaat tatacctaat attacaattc atgacctaag taatgtagtg
     1141 agtgaatctt atcataaggg agaatccatt gaggaattgg ctctttatgc cagggaaaaa
     1201 ttaggtattt caaaagataa tcatgatttg ctttataaat tagaacgttc aggcatctat
     1261 atcgtggaac gattaattaa tggccaagct gatgcttata gcgcatggtc aaaattggga
     1321 agaccttata ttgtgttggg aacgaataaa tcatctgtac gtcgaaattt tgacttagct
     1381 catgagctag gacatattct tttacataaa tataaagata tgaatgaaga tggcgatcgt
     1441 ttggagcaag aagcaaatta ttttgcatca tgttttttat tgccaaaaga agagttttta
     1501 gtcaaatttg aagagagggt tggcaagcgt gtcagcaatc ctgataatta tattttattg
     1561 aagtcggatt tgaatgtttc gatacaggct ttagagtatc gagcttttaa gttaggatta
     1621 ttgactccaa agcaacattc ttacttttat cgtcaaattg cgcaaaaagg ttacaaaatg
     1681 attgaacctt tggatgatca aatttttgtt aaaaaaccaa gcaaagtaaa gagtattctg
     1741 gacgtcgttt tgagtaatca tctagtcagt ctagcgacta taatgtctaa acaaagtatt
     1801 cgtttacagt ttataagcga aatattttca gtcgaaatga aattttttga tcagtatcaa
     1861 gaagatagaa gaacagatcg atttgataac atcatccctt tgtacaaaag aaataattta
     1921 taatttaact gtgaaataat ttcatgaagg atatatggca gaaattggat tttatcagac
     1981 aagcagtagc ttaccaaaaa taatcacaca agcagcaagt aagaggaaag gcacgaaagg
     2041 aagtctttcc tttttctttt gcaggagaaa ggccaggata cccgtcgcag aagcgaactg
     2101 aatcaagatc agtaactccg ttacgctaaa gacgagagcg caagaagcta aaaaaaggaa
     2161 atcccctgca cccatgcgga tatcgataaa atgagccaaa attccaagta tgaggaagga
     2221 gaccatgacc agattccagc cagaggaagc tattaggatt aggtggaaag tcatccagac
     2281 cagtaaggga tattcctgat ggcgaaagtc gtagatgccc aaggtcaaac cagcagtgat
     2341 taggatgact ttactcaagg aaagtaattc ccaagagtaa agcagaaaga ggagtcctaa
     2401 gcctagttca aagagggcat accagacagg atagcggacc ttgcagtagc gacagcaaaa
     2461 gcgattgaag acctgtgaga gaatcggaat caaatctaag ggacgcaagc gagtctgaca
     2521 ggaatcgcag tgactggctg aactgataat ggattgctct ggaaaacggt caatgaccaa
     2581 accaagaaag gaagctagaa tgctcccgac gagaaaaaaa taaaaatcaa tcatacttat
     2641 ctattcgtaa aaaatgggag aaatagtata atggagaaac atagataagg tggtgaggaa
     2701 aagatgacaa ttcgttttga agaaaaggtg agcacagaaa acgctcaatt tgtatgccag
     2761 tggtccaact ccctaggcaa atcctttcaa gaacaatgga tgggaccaag gattcctttt
     2821 ctactgaccc ttcaagcctt ggaaggagtc ttttcaatct ttgatgaaca agagtttgtg
     2881 ggatttatcc agaaaatcag gctagaagac agcaatcttc atatcgggag attttttatc
     2941 aacccccaga aacaggagca agacttaggt agccaggctt taaggaaatt tgttagtttg
     3001 gcctttgaaa atgaagatat agatagtatt tctctaaatg tcttcgaggc aaatcaaaga
     3061 gttcagaatc tttaccaaaa agaaggattt gaaatcgttc aaatggttga agcacctgta
     3121 cgaaaatata gtagattgaa actagaatag tacacctcta cttctaaaac attgttagaa
     3181 atc
//
LOCUS       DAVMGZ010000156         3143 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000156 DAVMGZ010000000
VERSION     DAVMGZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.157"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            215..2998
                     /locus_tag="VYQ93_001877"
                     /pseudo
     CDS             215..2998
                     /locus_tag="VYQ93_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT          951 a          466 c          706 g         1020 t
ORIGIN      
        1 gtctaacttt tggggtgtag ttcatgtaca cctgatatga tgcgtttttt gaattttata
       61 gactgctcgt ttaaactcta tttacctcgt acattccgga gcgagacgga atattagcca
      121 catacaaaat gagtactatt aggattttat tttcgtgtac aatttcagct attcttgtta
      181 taatcagcct ataggaatca aggaggtgac tcttatggct gtttttgtgt ctttggatgg
      241 aattgtggta gaagtacttg atgtcttttc ttcttttaat ggggatagtg agtttttctt
      301 gtgtaaacgt cttaaagaca agagtcagtt tgttatggaa cgctctcagt tcgaggagat
      361 gttccaactt caaagtagtc gcttgacgac gcaagaaaaa ttacaattgt ttacctccgt
      421 gtttgctgga cgttatgatg tttatgctaa gaattttatc aatgaacaag ggaaaattca
      481 gtattttcct tcctatgatt atggttggaa gcagttgcca cctgaaaaac ggagtttcca
      541 gacattgacg aactccgttt tgaaatctca ttttcgtggg gaggcagcta tcggtatctt
      601 tcctatgcac ttagatgata gctgtcattt tttggtactg gattttgatg aaggagattg
      661 gaaagaagct ggtttaacca ttcgaagaat agccagggaa cgccagatgg aagcccattt
      721 agagatttct cgttcgggtt acggactcca tatttggttc ttctttgagg aagcgattct
      781 gagtcgaaag gctcgcctgt ttggaaagaa actattagaa ttggctatgc aggaaagtat
      841 gcaactgtcc tttgattctt ttgatggcat gtttccaaat caggatgttc ttcctaaagg
      901 tggatttgat aatttaattt cccttccttt tcaaggagaa gcttatcatc aagggcgaac
      961 ggtctttgtg gatgaacatt ttcagcctta tgaagatcaa tggaggtatc tacaagaaat
     1021 tcagaggatt tcaactgcta aagtggcact gttaatccaa gaggagttag gcaagcaaga
     1081 attggataag gagttgaagg tcgttttatc caatatgatc caacttgaaa aatcgtctgt
     1141 gacatccaag gcactttttt tcttgaaaaa tatggcttcc ttttctaatc ccgaatttta
     1201 tagtggattg aaactagaat agtacacctc tgcttctaaa acattgttag aaatcgattt
     1261 gactttcctg atcgatttgt cctgttatta tttcatttta ctatatttaa agcaggctat
     1321 gcgacagcca acctatcaaa ttcctgagcg aatgtattta tttggagaat ccgattatta
     1381 tttatggctg ccaagaggct tgttatatcc attgcaagat aaatttaagc aggtagttgt
     1441 ggaggatagg agaaaagtac aaaggtctat tagagtagca tttaagggag agcttacttt
     1501 ggagcaagaa ttagccctgt cagatatgaa ttctaaagaa aatggtttac ttcatgcgga
     1561 gactggtttt gggaagaccg ttttaggtgc tgctcttatc tctgaacgga aaacaaaaac
     1621 aattattcta gtccataata agcaactctt agaccaatgg ctagatcgct taaactgctt
     1681 tttgactttc gaagaggagg aggctatccg ttatacggca tcaggtcgtg aaaaggtaat
     1741 cggctatgtt ggacagtacg gtgggactaa gaaatggctg agtaaactgg ttgatgtcgt
     1801 tatgattcaa tctctattta agttggaaaa tagtcaaagt cttttggatg agtatgagat
     1861 gatgattgtg gatgagtgtc atcatgtctc tgccttgatg tttgaaaaag ttgttgctca
     1921 gtttagaggg aagtatcttt acggtttgac ggctacgcct gagcgtaaga atggtcatga
     1981 gcctattgtt tttcagagaa ttggtgagat actccatact gctgataaga gggaaacgga
     2041 ttttaaacgg caattgcaat taagattcac ttcttttggt catttggaga ttgaaaagac
     2101 caaagcaagt aattttatac agctcagtga ttggattgct acagactcag cgaggaatca
     2161 gttgattctc aaggatattc tagcccaagt ggcagaagga cggaatatct tggttttagt
     2221 taatcgaatt caacagatag atgtctttga aaagttattg aaagagaaag aggttgatgg
     2281 ctgttacatt attagcggaa aaaccaaagt ccgagaaaga acgagtttac tggagacgtt
     2341 agaacagtta gataaagggt ttgttttgtt gtctactgga aaatacattg gcgaaggttt
     2401 tgacttacct cagttggaca cgcttatctt ggcagcaccc ttttcttgga aaaataattt
     2461 aattcagtat gcaggtcgga ttcatagaaa ctacaaggat aagtctttgg tgcgtatttt
     2521 cgattatgtg gatattcatg ttccttattt agaaaagatg tttcagaaac gacaagtagc
     2581 ttatcgaaag atggattatc gtgtcatcga gggtgaggag aaacaattcg tttatgttga
     2641 tagtagatat gagaaggtgt tgagagagga cttagcaggg gaaagacagg aatgtctgct
     2701 tattttacct tatgtgcacc agacaaaact gatgaatttt ctaaaagaat ttaggattag
     2761 tcaaattgag atatgtatac cagagacggt tgcaaataaa gcatggctag accagttgaa
     2821 gagccagaaa attaaagtgt cttttactca atcaaaaata gtaacgccta ttcttttggt
     2881 gaataagact attgtttggt atggtgcaat gccattatta gggaaggtag atgagatgac
     2941 catattacgt ttggaatcag ctagtatagt ttctgaacta gtggcaggtt tacgatagag
     3001 aaaattttta aaaatttcta tgtatgattt tcatttcttt agtgagactg ttgccattat
     3061 cacattcgaa tcacacaaaa taaaaaaatt tttataagta cttgacaaat agattgaaat
     3121 atcataaaat aaaaacggtt aca
//
LOCUS       DAVMGZ010000157         3089 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000157 DAVMGZ010000000
VERSION     DAVMGZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.158"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..177
                     /locus_tag="VYQ93_001878"
     CDS             <1..177
                     /locus_tag="VYQ93_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV3336515.1"
                     /translation="ENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSGVT
                     LITGKNGTGKSTLL"
     gene            complement(543..1853)
                     /locus_tag="VYQ93_001879"
     CDS             complement(543..1853)
                     /locus_tag="VYQ93_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV3336516.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2090..2962
                     /locus_tag="VYQ93_001880"
     CDS             2090..2962
                     /locus_tag="VYQ93_001880"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV3336517.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          949 a          518 c          703 g          919 t
ORIGIN      
        1 gaaaacagaa ctgtttcttt tgctattagt tcaaaaggag aaaaaatgaa agtagaaaat
       61 atttcgtata gggtggatca tcgtatattg tttgataata tttcttttga tacttcgagt
      121 tcaggcgtga cattaattac tggtaaaaat ggtacaggaa agtcaacttt actatagtag
      181 attgaaacta gaatagtaca catctacttc taaaatattg ttagaaatcg atttgactat
      241 cctgatctat ttgtcctgtt cttatttcat ttcactatat ctcaaattga gtatgacgaa
      301 gtgcgctccc atgtcctggg aacgcacttt cttcatattt ttcatattct tgaatccatc
      361 gataaagact attgggatga atttttaaag ttgaactaat catttttaca ggatgagatt
      421 tacagcagag agtttgaagg ctttatcaaa ggtttttctt ggcataatga cttttcctcg
      481 tttccactta attttgtgtc tactttatta taccaagtcc acccttaagt tagataataa
      541 atctaactta aggaagctag aaggatgaga atccaggtgg tcaagagtcc caaacttaag
      601 ctgatgggga cacccagaat aatttgcttt ttgaaggcaa ggccacgttc ctctatattg
      661 ggaagtgaga gttgaatgag agaaccagct gatgaaaagg gtgagatatt agtagataga
      721 gcgccaataa cggtggctgt tgtgagtaag tgaatatcaa tctgaggatt ttgagcactg
      781 atgatagcaa tgatgggaaa gagggctgga gctacaacgg atagggtgga actaaagagt
      841 gacatcactc cggctatcac acaaaagaac agaggtaacc agaaatgagg aatggttgtt
      901 gtcatgaggt gccctatcag tgtgactaaa cctgacttga ccgctagaga cattagtaag
      961 ctcatgccgc agagcatgat aattgtagcc cagggaacct tagctaaaat ggcttcttgc
     1021 ttccctaatt tgagccttaa ggcgaggcag accatgagta ttgagacaaa gccaatatca
     1081 aatgtttttt gataagtagc tatccaggcg atgtttggga aaatgagatg caacaaggga
     1141 aaaagccaaa ccaaaaccat gctgctgatc atgagcaagg tggtttgtct ttgaaccttg
     1201 ctgaggagtg gtggttggtc aatagtcaag gatgagtttg ttcttccctt actatagtga
     1261 ctgtaacagg ataataaaag caagacgatg agtgggtaga taatgctgac gataaagata
     1321 tgattgccaa gtgaaaaagc ttgctcttcc catcccattt gcttaaacag gccttgaaag
     1381 acaatgcctg agctactggt tatcaaatta gcccctcctg aagctcccca attgacggct
     1441 tgagctccaa tcaaagggtg tttgtccgct ttttgacaga gggtaatcgc tagaggacag
     1501 caaacggcca tagtagtgaa aaatccagca cctaaagcag acaaaagggt tgccatcagg
     1561 tataaaatca tgtagagggc gttagggtgg gtgcgtgtgc ggtagagaat gtgttgagcc
     1621 aaaacatcaa gagtaccgtt agttgttgca acgttataaa agagagagac gctaaaaatg
     1681 gtaaaaaaga gtgaggttgg ccaaaaatga agaagttctt tggggcttaa tcccatgaga
     1741 gtggttgcga tgaggtaaga aaaagcaata gccagcaggc caatattgat tttggtgcgg
     1801 taaccaattc caatggctag agcaatggcg ctaatcatta ttaaatgaat cattgaattg
     1861 tcctttcagt tagaatatgg aaataggttg gcaattttgg cgctcaaaag accacctgtt
     1921 tcctattggt acttgactat tatacacaaa aaaaatacaa ttgtcttgac aattgtattg
     1981 acacttgtta gaattaaaaa tgaggtattc aggcttgtga tttcctatgg aagttaatag
     2041 taattgcctc tagtgcttac aagtgatatt aaaaatagag gacttagtga tgtcaatcat
     2101 ttcaactgat ttaacccctt ttcaaataga tgatacattg aaagcagcct tgcgagaaga
     2161 tgttcattcc gaagattaca gtaccaatgc catttttgat catcatggcc aagccaaggt
     2221 gtcgcttttt gccaaggaag ctggtgtttt agcgggacta accgtttttc aaagggtttt
     2281 taccctattt gatgccgagg tgaccttcca gaatcctcat caatttaagg atggggatcg
     2341 tttgactagt ggcgatttgg ttttagaaat cataggctcg gtgagaagtc tcttaacatg
     2401 tgaacgcgtt gccttgaatt ttttacaaca tttatcaggg attgcttcga tgacagctgc
     2461 ttatgtagaa gccttaggcg atgattgcat taaggtattt gatactcgaa aaactactcc
     2521 taatttacgt ctttttgaga aatatgccgt gagagttggc ggtggctata atcatcgctt
     2581 taatttatca gatgctatca tgctaaaaga caatcacatt gcggcagtag gtagtgttca
     2641 gaaggcaatt gctcaagcgc gtgcctatgc cccttttgtg aaaatggtcg aggtggaagt
     2701 ggaaagcctt gctgctgccg aagaagctgc ggcggcgggt gctgatatta tcatgttgga
     2761 taatatgtca ttggaacaga ttgaacaggc cattacccta attgcaggac gttctcggat
     2821 tgaatgttct ggaaatattg atatgaccac tattagccgt tttcgtggtt tagcgattga
     2881 ttacgtctcc agtggtagtt taacccatag tgctaagagt cttgattttt ccatgaaggg
     2941 tttaacctac cttgatgtct aagttgtaaa ataaactaac tttttaaagg atgtctttcc
     3001 tctagaacga gttttatgtc agatagttta aacgcctctt caaatatagt aaaatgaacc
     3061 aaaaatagta cacaatgtgg tataatctt
//
LOCUS       DAVMGZ010000158         3053 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000158 DAVMGZ010000000
VERSION     DAVMGZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.159"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            30..1142
                     /locus_tag="VYQ93_001881"
     CDS             30..1142
                     /locus_tag="VYQ93_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336518.1"
                     /translation="MQMEIKAYNLSKDIKDDLYKKMEDSERVHLKAEVTTILTLSISS
                     IFSFISLAVVIFVGVNLIINKEINSLYLIGYLLAAMKIKDSLDASKEGLMEIFYLSPK
                     IERLKEIQNQDLQEGDDYSLKKFDIDLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVG
                     ASGCGKTTILKLISRLYDYDKGQILINGKDIKEISTESLFDKVSIVFQDVVLFNQSVM
                     ENIRIGKQDASDEEVKRAAKLANCTDFIEKMDKGFDTVIGENGAELSGGERQRLSIAR
                     AFLKDAPILILDEITASLDVNNEKKIQESLNNLVKDKTVVIISHRMKSIENADKIVVL
                     QNGRVESEGKHEELLQKSKIYKNLIEKTKMAEEFIY"
     gene            1153..1737
                     /locus_tag="VYQ93_001882"
     CDS             1153..1737
                     /locus_tag="VYQ93_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV3336519.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            1737..2432
                     /locus_tag="VYQ93_001883"
     CDS             1737..2432
                     /locus_tag="VYQ93_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV3336520.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VVGLIVGGLIWLK"
     gene            2420..>3053
                     /locus_tag="VYQ93_001884"
     CDS             2420..>3053
                     /locus_tag="VYQ93_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336521.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFL"
BASE COUNT         1101 a          394 c          580 g          978 t
ORIGIN      
        1 actcagaaag ctttcaagaa aatatcgaaa tgcaaatgga gattaaagca tataatttat
       61 cgaaggatat taaagatgac ttatataaaa aaatggaaga tagtgagaga gtacacttaa
      121 aggcggaagt aactacaatt ttaactttgt ctatatcttc aatatttagc tttatatctc
      181 ttgctgttgt gatatttgtc ggcgtaaatc taattattaa taaagagata aattctctct
      241 accttatagg atatttacta gctgctatga agataaaaga ctctttagat gcatctaaag
      301 agggcttgat ggaaatattt tatttatcgc ccaaaataga aagattaaaa gaaattcaaa
      361 atcaagatct acaagaaggc gatgactata gcttaaaaaa atttgatatt gatctaaaag
      421 atgttgagtt tgcctacaat aaagacgcaa aagttttaaa tggtgtaagt tttaaagcta
      481 agcagggaga ggtcactgct ttggtaggtg caagtggctg cggtaaaaca actatcttga
      541 aacttatatc aagactttat gattatgaca agggacaaat cttaatcaat ggcaaagata
      601 taaaagaaat atcaacagaa tctctttttg ataaggtctc tatagttttc caagatgtgg
      661 ttctctttaa tcaaagcgtt atggaaaata ttagaatcgg aaagcaagat gcaagtgacg
      721 aagaggttaa aagagcagca aaacttgcaa attgcacaga ttttatagaa aaaatggata
      781 aaggtttcga tacagttatt ggtgagaacg gagctgagct atcaggagga gaaagacaaa
      841 gattatcaat agccagagcc ttcttaaaag atgcgccgat attgatctta gatgagataa
      901 cagcaagcct tgatgttaac aacgagaaaa agattcaaga gtctttaaat aatttagtta
      961 aagataaaac tgttgtaatc atttcacata gaatgaaatc catagaaaat gcagacaaga
     1021 tagtagttct tcaaaacgga agagtagaaa gcgaaggtaa gcatgaagag cttttacaaa
     1081 aatcaaaaat ttacaaaaat ttaatagaaa agacaaaaat ggcagaagaa tttatttatt
     1141 aggaggacta caatggataa taaaaaatta aaagtaaaag atttagtaag catcggtgtt
     1201 tttggcgtaa tttattttgc cttcatgttt ggagttggta tgatgggctt gattccaata
     1261 ttgttcttaa tatacccgac agtattagcc atagttgcag gaactgttgt tatgttattt
     1321 atggctaagg ttcaaaagcc atgggcacta tttatatttg gtatgatatc accacttgtg
     1381 atgtttgcag ctggtcatac ctacgtagtt gtggttttat cacttatagt aatgataata
     1441 gcagaattaa ttagaaagat tggtaattat aattcattta aatacaatat gctttcttat
     1501 gcaatcttca gcacatggat atgtagctct ttaatgcaaa tgcttttagc aaaagaaaaa
     1561 tatatggagt ggtctttgat gacgatggga aaagattatg ttgatgtatt agaaaagtta
     1621 ataacttatc ctcacatggc tttagtagcc ttaggtgctt tcttaggagg aattcttgga
     1681 gcatatatag gcaaggctct attgaaaaaa cactttgaaa aagcaggcat tgtataatgc
     1741 ctactttttc ttcaaatgct ccctttaatc ccaatccgat ctctaagata ttagtcgtat
     1801 ttatcacagg cttaactgtt atgcatagca taaacatccg ttttgagctg gcaattgttt
     1861 gtcttattgg tattttgctt tatttgaatg gatacaaaaa aacacttttc aaatggatta
     1921 tattgtgtgg gatactttac tccttgccta attttatggt gttatctgaa ttaaacccta
     1981 tagttaagat gtttttgagt atacctattg ttattagaat gtttatttta ccatttatgg
     2041 cagcaagctt tatgataaag acctcggatg taggcgcaat aatttcatcg atggataagc
     2101 ttaagatttc aaagaatgta tccataccta ttgcggttat gtttagattc ttcccatctt
     2161 ttaaggagga gaagaaaaac atcaaaatgg ctatgagagt aagagggata aattttaaaa
     2221 acccagtcaa atatcttgaa tatgtttctg tgccactact cattatatca tctaatatat
     2281 cagatgacat tgcaaaagcg gcagaaacaa aggcaataga aaatccaatt gccaagacca
     2341 gatacattcg cgtaaagata cagctaattg attttgttta tgttttagcg gttgttggac
     2401 ttattgtggg aggcttaata tggttgaaat aaaaaattta agtcttgatt atggtgaaga
     2461 gcatatatta gatgatatat cactatccat agccgaggga gagtgcgtgc tatttacagg
     2521 aaaaagtgga aatggtaagt catctttaat aaattcaatc aatggactag ctgtaaggta
     2581 tgacaacgca aagacaaagg gcgaaatcat tattgatggt aagaatataa aaaatttgga
     2641 actttatcaa atctcaatgc ttgtttcaac tgtttttcaa aatcctaaga catatttttt
     2701 taatgtcaat acgacattag aattattatt ttatttggaa aatatcggtc ttgcaagaga
     2761 agagatggac aggcgtttga aggatatgct taagatattc ccgataaaaa atcttttgaa
     2821 cagaaatata tttaatctat ccggcggtga aaaacaaatt ctttgcattg cagcttctta
     2881 tatagcaggt acaaagatta tagttatgga tgagccttca tcgaatttag atattaaaag
     2941 cataagtgtt ttggcaaaga tgctaaagat attaaaagag aaaggtataa gcataattgt
     3001 tgcagagcat agaatttatt atttgatgga catagttgac cgtgtatttt taa
//
LOCUS       DAVMGZ010000159         2960 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000159 DAVMGZ010000000
VERSION     DAVMGZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.160"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(143..676)
                     /locus_tag="VYQ93_001885"
     CDS             complement(143..676)
                     /locus_tag="VYQ93_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV3336522.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(765..1541)
                     /gene="recX"
                     /locus_tag="VYQ93_001886"
     CDS             complement(765..1541)
                     /gene="recX"
                     /locus_tag="VYQ93_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV3336523.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1579..2934
                     /gene="rlmD"
                     /locus_tag="VYQ93_001887"
     CDS             1579..2934
                     /gene="rlmD"
                     /locus_tag="VYQ93_001887"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV3336524.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT          936 a          531 c          542 g          951 t
ORIGIN      
        1 ttttacgaat tctgcataaa tctctttcta gtaatgtgtt gtaactctgc tataatggat
       61 ttattccttt ttgtatttac acaatttatt ttatactacc aaaaaaacaa ggtcaggatt
      121 ttgttcctga cctttgacaa ctttaccgat tctttagttc tacatagcgc ttgtaccaaa
      181 tgtttacata ggcttctgag aaaggaccac gtccattgtt aatccaatca acaagaattt
      241 tgacatgttc ttttaaaata tagtccaagt catcagaata attcattttg cgtttgtgac
      301 gctcgtattc ttcaacgtcc aagagacgtt tttccccatc tgtaaaaatt ttaacatcca
      361 aatcgtaatc aatatacttc agtgcttctt catccagata gtaggggcta gccatattgc
      421 aatagtaaga agttccatta tcacgaatca tggcaatgat attaaaccaa tatttcttgt
      481 gaaagtaaac aatagccggt tctcgagtga cccaacgacg accatcactt tcggtaacaa
      541 gtgtatgatc gttgacacca ataatggcgt tttctgttgt ttttagtacc atggtgtccc
      601 gccaagttcg gtggagactc ccatcatgct tataactttg aattgtaata aagtcgcctt
      661 cttttggaag cttcataact aaccaacttt ctacaattta taagtttatc atttactatt
      721 gtaccataaa attacctaaa atctgtgaat ttcacttgga aatattaaag atattctcta
      781 agagcgcttg ctatatccga aaaatcgtag ccctttcgtg ctaaaacttg agttaaacgc
      841 tgcttcagtt cgtatccttc atactttcga gcatacttag tatattgctt atcaagttcc
      901 ttgaagatga gttcttgagt cgtttcttgg tcaacttggc tatctaagtc gtcaaaggca
      961 attttagcat cagaataaga gaagcccttg ttagtcaagt tctggataat cttatcttgc
     1021 agggcacgag ctggaagttt tccctcatat tttttcaata gtttattggc tacacgttga
     1081 gcaacttcag aaaaatcaaa ttctttcaag ttctcttcta tagtagattt tgaaatccct
     1141 ttttgagata gtttctgagt cagcacataa ggacccttgt ctcctgaaag ttgattggta
     1201 ttgatgatag cataagcgta ctgaccatca ttaatccact tatcttcttt aagattagca
     1261 ataacttgag aaacgatgtt tttatcaata tcgtattttt tcagatattc tctgacttct
     1321 ttttcagtgc gtgctttaaa ggataagtgg tagagagcga gattcttacc ataagaaaat
     1381 tgagcaaagt cttgaatctc tttcaattcc tcttcgctta tcaccttatc tctcgataac
     1441 ataaaacgaa caattgtatc ttcggtgata tagcatttgt cgccattatc aagctccatc
     1501 aggtagagtc tttttttctt ttcaagtttt gtgattttca tagctctatt ataactcaaa
     1561 atgtgataag ataggggtat gaatctgaaa gtgaaacaaa aaataccatt aaaaatcaag
     1621 cgcatgggaa ttaacggtga gggaatcggc ttttaccaaa aaacattagt ctttgtacca
     1681 ggagctctca aaggcgaaga tatctattgt cagattactt ctattagacg caactttgtt
     1741 gaagcaaaat tactgaaggt caacaagaag tctaaatttc gaattgtgcc atcttgtact
     1801 atttataatg aatgcggagg ctgccaaatc atgcacctgc attatgataa gcagctggag
     1861 ttcaagacgg acttacttca tcaagcgctg aaaaaatttg ctcctgcagg atatgaaaat
     1921 tatgaaattc gtccaactat tggaatgcag gaaccaaaat attacagagc taagttacaa
     1981 tttcagactc gaaaatttaa aaatcaggtc aaggcgggct tatatgcaca aaactctcac
     2041 tatttagtag agttgaaaga ctgcctggta caagataagg aaacccaagt gattgctaat
     2101 cgcttagcag aattacttac ttatcaccag attccaatca cggatgagag aaaagttcta
     2161 ggtgtccgta ctattatggt ccgacgcgcg agaaagaccg gacaggttca gattattatt
     2221 gttacaaacc gccagcttaa tttaactcaa ttggtaaaag agttggttaa agatttccca
     2281 gaagttgtga cagtagctgt taatacaaat acagctaaaa ccagtgagat atatggtgaa
     2341 aagacagaga ttatctgggg gcaagagagt attcaagaag gtgtactcaa ttatgaattt
     2401 tcactatccc ctcgagcttt ttatcaacta aatcctgagc aaacagaagt cctctatagc
     2461 gaagcagtaa aagcgctgga tgttgataaa gaagaccatt tgattgacgc ttattgtgga
     2521 gttggaacga ttggatttgc ctttgcaaag aaagttaaaa cactcagagg tatggatatt
     2581 attccagaag ctattgaaga tgccaagcga aatgctaaaa gaatgggatt tgacaatact
     2641 cattatgaag ctggaacggc agaagagatt attcctcgtt ggtacaagga aggctaccga
     2701 gcagatgctt tgattgttga cccaccacgt acaggtctgg atgataagtt attagatact
     2761 attcttactt atgtaccaga aaaaatggtt tatatttctt gtaatgtttc gaccttggct
     2821 cgtgatttgg tacgcttagt agaagtctat gatcttcatt atatccagtc ggtcgatatg
     2881 ttcccacata cagctcgaac tgaagctgtt gtaaaattaa taaaaaaagt ttaaaaaagt
     2941 agttgacaaa gtttgaaaag
//
LOCUS       DAVMGZ010000160         2884 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000160 DAVMGZ010000000
VERSION     DAVMGZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.161"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..357)
                     /locus_tag="VYQ93_001888"
     CDS             complement(<1..357)
                     /locus_tag="VYQ93_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEV3336525.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFF"
     gene            complement(475..888)
                     /locus_tag="VYQ93_001889"
     CDS             complement(475..888)
                     /locus_tag="VYQ93_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV3336526.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(885..1121)
                     /locus_tag="VYQ93_001890"
     CDS             complement(885..1121)
                     /locus_tag="VYQ93_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV3336527.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            complement(1181..2242)
                     /locus_tag="VYQ93_001891"
     CDS             complement(1181..2242)
                     /locus_tag="VYQ93_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3336528.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIIVPYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYG
                     GNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVF
                     LLELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQNEFADFVALVDKSQLAIQ
                     KSLEELETLKKSLMQEYFG"
     gene            complement(2276..2809)
                     /locus_tag="VYQ93_001892"
     CDS             complement(2276..2809)
                     /locus_tag="VYQ93_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3336529.1"
                     /translation="MKETYTDEHLEIMRDNCENLRDLAIIDLLASTGMRVGELVQLNR
                     SDIDFENRECVVFGKGKKERPVYFDARTKIHLRNYLNDRKDSHPALFVTLVGKAQRLG
                     IAGVEIRLRKLGDKLGIQKVHPHKFRRTLATKAIDKGMPIEQVQKLLGHSKIDTTLAY
                     AMVNQNNVKHSHQKFIS"
BASE COUNT          863 a          564 c          415 g         1042 t
ORIGIN      
        1 aaaaaaagaa gacaatatac gcctaatatt gtctaaattt gccttactac aatccttttc
       61 agactggtaa tttgctaaat aactccttaa atcatctgtt gtgagttgtg tcacagactc
      121 tttaatagca ttaaacaagt tttcaatcgt cctctggtaa taacgaattg tttttgagct
      181 acagccctct acctgcttgg cggtaataaa tagttgcaac aggttattat tcgaatactt
      241 ctgctgcttt tttccgtcta ctagatgatg ttctaaactc tctctcaacg ctagtaattg
      301 ttcattattt aaaagaggta acatttcttg catgataatt gtaacttttg attccataat
      361 ttctcacttt cttattcatc aataaaaaga gacaatatca gtttctgcat tacagaattc
      421 taatattgtc tgttcattta ttttcttgtg ctcattcaaa tccagcatac taggttactt
      481 tttgaccttt tctctaacag aatgttcaga aacccatttg gagtagcttg tcattttttc
      541 atcagttaaa gcttctactg cgatggttac acacattttt actgcttctt ctaccgttac
      601 agaaaaacga tagccgttta attgtaaaaa tttgaccaaa acgaagaaag cagttctttt
      661 attagcatta gcaaaaacat gcttctttat caattggaca aatagtatcg ttgctttatc
      721 aaaaattgtt ggataaagag acttcccaaa gacaaattgt tctggtaagt tcacaatcat
      781 atttaaggca gaagaactaa ctgtttgaat tttctcattt ggagaatacc gttgaattgc
      841 taaagcattt attttttcaa tttgcttttc tgtcagatag attgtcattt ttccaccaaa
      901 gctttgaaaa catcgtcgta ttcgtcaaat attttatcta aatttaagtc aaaggcatca
      961 tcagatacat aagaaacctg ctgccccaat tcttcaggag taaagataat ctcaccactt
     1021 ggtaacaatt tcgcttcgta tttaacacca cttggaatat taaattcact tggaatcgta
     1081 atggtgattg aatttccttg ttttcttgtt tttactacca tctgatttcc tcctttgaag
     1141 tattataaca aatcattacc gtaattacaa tggcagaata tcagccaaaa tactcctgca
     1201 tcagagattt cttcaaagtt tcaagttctt ccagagattt ttggattgcc aattgtgatt
     1261 tgtcgaccag ggctacaaag tctgcgaact cattttggag ggctagaggg gggaggatat
     1321 atttctgatt ttctaatggt ttttgagtta tctttggttg acctgagaaa tcagcaaatt
     1381 tactaaaatc aattactttc attaattcta ataaaaatac aagattgaaa tcggaattct
     1441 taaattcttt tatatatata gcattgtcac ttatccatac cttaccgtgg gtagttctta
     1501 cattaccaca atatgcacct acacgaccaa tgatgatagt aggattatca attaaatatt
     1561 ttcgactttt ccaagcaatg ccatttccac cataggcagg ataaccttca aaaactctat
     1621 catgtttatc aagaaatttt ccacttgaaa atttaaaaca ctgttgtccc gtttttactg
     1681 caaatttctt gttattattt aaaggatccc caaacatctc gttaaatcgg gattttacaa
     1741 gtagattaag ctcttcaagt tgttcttgtc tccttaaaat cagcttgctt aacaaatcca
     1801 gctcacttgc aattctttgt tgctctccca aatttgtata tggaactata atattatcaa
     1861 aatatttttt agttaaatgt ttcattgtgg aaccatgagt atgttttaca gcatcttgta
     1921 aacctttttc aacaacatat ttaaaatacg atttatctat atctatctta tcaaaaacca
     1981 ctttaaaaat gtgctgattt aatactgcgg atcttccaca ccactgaaaa acccctaatg
     2041 tacccgacca cgaaataaga atatcaccag cttcaacaat gtatttttta tcaatcgtac
     2101 ccgaataata atttatccct tttgaagttt tagtcagatt ctgtattctg ataatctctt
     2161 tcccttcaga tgaccaatct tgaggtttaa aagcatagcc attgataaaa gtagccactt
     2221 gccctaattt cactttcttc atgcaaccac ctcctcggaa tatcgggatt tgcttttaag
     2281 agatgaattt ttggtgtgaa tgcttgacat tattttgatt gaccatggca taggccaggg
     2341 ttgtgtcaat cttgctatga cctagcagtt tttggacttg ttcgataggc atacctttat
     2401 caattgcctt agtcgctaaa gttcttctga acttatgtgg gtgaaccttt tgtatgccga
     2461 gtttgtctcc taactttctt aagcgaatct ctacaccagc aattccaagt ctctgggctt
     2521 ttccaactag cgttacaaaa agagcagggt gactatcttt tctgtcgtta agataatttc
     2581 ttaaatgaat tttcgtccga gcgtcaaaat atactggtct ctccttcttt cctttaccaa
     2641 agacaacaca ctctctgttt tcaaaatcaa tatctgaacg attcaactgt acaagctccc
     2701 ctacacgcat acctgtcgat gctagtaggt ctattattgc caaatctctc aaattttcac
     2761 agttatcacg cataatttcc aaatgttcat cagtataagt ttccttcaca ttttgctcag
     2821 tcttaatttt ctttatccgt cgaatgggat ttttaatgat atattcctct tgctcaagcc
     2881 aagc
//
LOCUS       DAVMGZ010000161         2654 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000161 DAVMGZ010000000
VERSION     DAVMGZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.162"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..178)
                     /locus_tag="VYQ93_001893"
     CDS             complement(<1..178)
                     /locus_tag="VYQ93_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV3336530.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYK"
     gene            complement(346..990)
                     /gene="pcp"
                     /locus_tag="VYQ93_001894"
     CDS             complement(346..990)
                     /gene="pcp"
                     /locus_tag="VYQ93_001894"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV3336531.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(1005..1928)
                     /locus_tag="VYQ93_001895"
     CDS             complement(1005..1928)
                     /locus_tag="VYQ93_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV3336532.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPTALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(1925..2614)
                     /locus_tag="VYQ93_001896"
     CDS             complement(1925..2614)
                     /locus_tag="VYQ93_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV3336533.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
BASE COUNT          871 a          582 c          395 g          806 t
ORIGIN      
        1 ttttataaaa actatcgaca ttcccattct ttataatggc aaattgtccg tctaattcgg
       61 caatttctcc gataactttt ttaccgatag tcaatgtata accttcaaaa ctgtctttac
      121 caacattaac tttggcatcc gctacttgaa tttcgatttt tttatctttc ttgctcattt
      181 catacctctc ttcacttttt ctattttaca agaaaatcct aaaactagca agaaaaaact
      241 ctatatcagg cttctctgat atagagtttt atgtaaacaa cctcaatcga tttagcatag
      301 ctaatagtta gtaaacaagc ataattgact atccttttta ggttatcagt gagtctcacc
      361 gcctactaac tgcaaatcct tatctttgta gtctacaata gcttcaatag ctacctctat
      421 gcctcttaca atatcacata gactcattcc tgcagtattc ggcttattta ctacttgttc
      481 catcatatat ggaatatgca tgaaacctgc tctcatatta gggaattttt tatccactaa
      541 ataaagagct tggtacatca aatgattgca aacaaaggtt cctgcactat tggatacaac
      601 tgccggaagt ccctgttttt tgatagcttg taccatcgct ttgataggta aactactaaa
      661 ataggccgat gctccatcaa tacgaatcgg tgtatcaatt ggttgattgc cttcgttatc
      721 aggtatgcga gcatcatctt gattaatagc cactcgttca ggtgttaagc cggtcctgcc
      781 gcctgcttgt ccaatacaaa gtacagcatc tggttgatat cgtaatattt ctgcctctaa
      841 aacttctgac gacttataaa aaaccgttgg aatttctacc cagcgaactt cagccccatt
      901 aatctcagat ggtaataatt ttacagcctc caaagctgga ttaatctttt cacctccaaa
      961 aggattaaaa cctgtaacca atattttcat tttattttcc tttactaaaa tgcgagaaag
     1021 tacattaaga atatgtgaat aacaatcatt actagagcaa cacctgcttg agcctttata
     1081 acgccattct gatctttcat atccatcaat gctgttggta gagcgttaaa attagcagcc
     1141 attggggtca ataaggtccc acaataacct gctgtcatgg caagagcacc agccacaatt
     1201 ggattagctc ccagagcaaa tacaaaggga actccaacac ctgctgtaat aacggtgaat
     1261 gctgcaaaag catttcccat aatcattgtg aatagaacca ttccaagaac ataggccaaa
     1321 actcctataa agcgactatc tgaaggaaca ataccgctaa tcagatgaga gataacatca
     1381 ccaacacctg ctacagtaaa aatagccccc aaagccccta ataattgagg aacaatacca
     1441 cttgttgaaa cttgctgagt cattcgatta ttttctgata acagactctt agggtgacta
     1501 ttggtaatca caagaacaga aattgtagca aacaaggcgg caaggctaat cgaaatcttg
     1561 ctaaattctg gaatcatttg cgctaagacc aacgcaagta ttgccatcag cataactgga
     1621 ataaaaattt tatttttcaa cctgttagat tcaatattgg ctttcatttc atctaaggat
     1681 ggcaaggttc cgatacggac ttgcttaaac aatgttaaca gcgataatag gattacaata
     1741 ataccaatac tcatatttgg catataggaa ccacctataa acgtaataga caatagagtc
     1801 caaaatgcag atgtcccaag tcgaactggg tttgttttat ctttataact acaataggct
     1861 gtatggagaa attgacaacc aatcacaata taggtcaact ctaatagttg ctttgccaac
     1921 tctgtcattt ttgttctcct cccctagtct tttttgatat caatttttta tcaaataaat
     1981 aattgtaaat ccccactaca ataagtgtta taacagcaac aataatagat gtagaagcaa
     2041 tccctgcata attgctttca tagcctaact gatctaatgt tccccctatc aagaggactc
     2101 ccccagcacc tacaaacgta ttttgagcaa agaaatttcc aaaattttca ttcgcagccg
     2161 cacgcgcttt tattgtctca tcttcaacct ctgttaactt tctacctaat tgagactctg
     2221 cagctgcttc tcccataggt tgaaccaaag gtctgacaaa ctgagggtgt cctcctagac
     2281 gaattgaaaa gaaaccagct aactctcgaa taaaaaaata aactgtatag aagtttccaa
     2341 ctgtcagacc tttaatcttt cgaatcaaat cgattgatcg ttgcttgagt ccaaaggttt
     2401 ctgacagccc cacaagaggc aaggtaacca taaaaatcgt gagcactcgc tgattgctaa
     2461 attcttttcc caaaatctcc aaaaattcaa cgagagaaac acctgaaact aaagctgtaa
     2521 ccaaaccagc taagactact gttgcaattg tatcaaattt taaaataaaa cccacaacaa
     2581 tgattgctat tcctattaat ctaatccact ccatatcaaa ctcctttata ttcaaaatga
     2641 cagtatttta aaaa
//
LOCUS       DAVMGZ010000162         2535 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000162 DAVMGZ010000000
VERSION     DAVMGZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.163"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..356
                     /locus_tag="VYQ93_001897"
     CDS             <1..356
                     /locus_tag="VYQ93_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV3336534.1"
                     /translation="DGKIDIDGENVFANCMTYLADGVPGDIFETHKKYLDIHIVVENT
                     EKMAVTSPVRAQSRVPFSEEKDIAFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCND
                     ETVKKLVIKVLNEEK"
     gene            358..>2535
                     /locus_tag="VYQ93_001898"
     CDS             358..>2535
                     /locus_tag="VYQ93_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV3336535.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPV
                     APTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     KELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNV"
BASE COUNT          880 a          389 c          504 g          762 t
ORIGIN      
        1 ttgatggaaa aattgatatt gacggggaaa atgtttttgc aaattgtatg acttatctag
       61 ctgatggagt tccaggagat atttttgaaa ctcataagaa atatttagat attcatattg
      121 ttgttgaaaa tactgaaaaa atggcagtaa cttccccagt tcgtgcccag tcacgtgtac
      181 cttttagcga agagaaggat attgcttttt acgatagcaa agactatcaa attgttgaat
      241 tgcttccagg gaatatgttg gtgacttttg aagaagatct tcaccaacca aagattcatt
      301 gcaatgatga aacggttaaa aaacttgtta tcaaagtttt aaacgaggaa aaataaaatg
      361 aaaaaaaata ttaaacaata tgtaacctta ggtactgtag tggtattatc agcatttgtt
      421 gctaactcag ttgcagctca ggagactgaa acttctgaag tatcaacacc aaagttggtg
      481 caacctgttg caccaacgac tccgatttcg gaagtacaac ctacatcgga taactcttcg
      541 gaagttactg tacaacctcg aacagttgaa actactgtta aggatccatc ttctacagcg
      601 gaagaaactc ctgtcttaga aaaaaataat gttactttaa cagggggcgg agaaaatgtt
      661 actaaagagt taaaggataa atttactagc ggtgacttta ctgtagtgat taagtacaat
      721 cagtcaagtg agaaaggctt acaagctctg tttggaatat ctaattccaa acccggtcaa
      781 caaaatagtt atgtagatgt gttccttaga gacaatggtg agttggggat ggaagcgcgt
      841 gatacttctt ccaataaaaa taacctagta tccagacctg cttcagtttg gggtaagtac
      901 aaacaagagg ctgtgactaa cactgttgca gtagtagcag attcagtcaa aaaaacatat
      961 tctttatacg caaatggtac aaaagtagta gaaaagaaag tggataattt cctaaacatc
     1021 aaggatatta aaggtattga ttactatatg cttgggggag tgaaacgtgc aggaaaaacg
     1081 gcgtttggtt ttaacggaac actagaaaat atcaaattct ttaatagtgc attggatgaa
     1141 gaaactgtta aaaagatgac aacaaacgct gttactggac atttaattta tacggctaat
     1201 gatacaacag gttctaacta tttccgtatt ccagttctgt atacttttag caatggtcgg
     1261 gtattttcaa gcattgacgc tcgttacggt ggaactcatg atttcttgaa taaaattaat
     1321 attgctacaa gttatagtga tgataatggt aagacatgga ctaaaccaaa attaacattg
     1381 gcattcgatg attttgcgcc agtaccatta gaatggcctc gtgaagttgg tggacgtgac
     1441 ttacaaatca gcggtggtgc aacctatatt gactctgtta ttgttgaaaa aaagaacaaa
     1501 caagtactca tgtttgctga tgtgatgcct gctggagtaa gttttagaga agcaactaga
     1561 aaagattcag gttataaaca aattgatggt aattattacc ttaaattaag gaaacaaggt
     1621 gatactgatt acaattatac tattcgtgag aatggtactg tatacgacga tcgtaccaac
     1681 agaccaactg aattttcagt agataaaaat ttcggtatta aacaaaatgg taattatttg
     1741 acggtagagc aatattcggt ttcatttgaa aataataaaa agactgaata tcgtaatggg
     1801 actaaagtcc atatgaatat cttctacaaa gatgcattgt ttaaagtagt gccaacgaac
     1861 tatattgcat atatttctag caatgatcat ggcgaatctt ggtctgcacc aactttatta
     1921 cctcctataa tgggacttaa tcggaatgcg ccatatttag gtcctggacg tggaatcatt
     1981 gaaagctcaa ctggacgtat tcttattccg tcttacactg gtaaagagtc tgcgttcatt
     2041 tatagtgacg ataatggagc atcttggaaa gttaaagtag tgccacttcc ttctagttgg
     2101 tcagcagaag cacaatttgt agaattgagt ccaggagtaa ttcaagcata tatgcgtaca
     2161 aataatggta aaattgcata tttaacaagt aaagacgcag gtactacttg gagtgcaccg
     2221 gaatatttga aatttgtttc aaatccaagt tatggaacac aattatcaat catcaattat
     2281 agccaattga ttgatggtaa aaaggctgtc attttaagta ctccaaactc cacaaatggt
     2341 cgtaaacacg gacaaatttg gattggtcta attaatgatg ataatacaat tgattggcgt
     2401 tatcatcacg acgttgatta tagtaactat ggatactcat attcaacatt gacagagtta
     2461 ccaaatcatg aaattggatt gatgtttgaa aaatttgatt catggtctcg taatgaactt
     2521 catatgaaaa atgtt
//
LOCUS       DAVMGZ010000163         2527 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000163 DAVMGZ010000000
VERSION     DAVMGZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.164"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            158..355
                     /locus_tag="VYQ93_001899"
     CDS             158..355
                     /locus_tag="VYQ93_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEV3336536.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            423..1088
                     /locus_tag="VYQ93_001900"
     CDS             423..1088
                     /locus_tag="VYQ93_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3336537.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSALVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIVGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            1160..2386
                     /locus_tag="VYQ93_001901"
     CDS             1160..2386
                     /locus_tag="VYQ93_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3336538.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT          664 a          450 c          486 g          927 t
ORIGIN      
        1 aatcgcaaat aagaaatttc tataagaaaa aatatcaaat atgcgatttt ttaaaataag
       61 ccaattttcg tgttatactg tacttgtaaa gcacttgaag caaatcctag gtcgcagaag
      121 tggtttacaa atgaagataa ttgaaggagt gtaaaagatg ttaaatttac aatttgcaga
      181 aacaatggaa ttgacagaag ctgagttgga gacagtttat ggaggggaat ttgggaataa
      241 tgctgttatc ccagccggtg cttggggagg tttaggaacg tcttggtcaa tcactaattt
      301 ctggaagaaa tattttaacc atgattcttc cactgttaat cgtcgccatt attgatattc
      361 agagtccttg cctttttctt gggtagggac tgtctttatt tcatagtata ggagaaaata
      421 gaatgaaaaa gataatctca caccgctact ttattatagt ttttctacta gttattgctg
      481 accagaagtt cagtgctcta gttttacgca gtgaccttgt tactgggcta tctgactttg
      541 cctattatct gtcggatatg atgttgaatt ttcttgtggt tttatttgct cttattgcta
      601 tgatttggtc ggggaaatgg cagaaaatca atagtagaaa gtttaaagga tcctatcttt
      661 tctattcctt cctagctctg cttgcttttg ttgtttggaa tttcgttacc ttttttcttt
      721 tcccacctac tcgaaatgaa atttcttatc aacatgctgc tccgactttt acaggggcta
      781 cagcattttt gatgtatttt ttctatcctg tgattgtagg tcccattttt gaagagataa
      841 tccatcgggg attggtgatg acagctctgg aaaaaggaaa gaaatggggg ctagatgtac
      901 ttggttccgc tgttttattt ggggtctcgc acattagtaa tcacggttgg gtcttgacag
      961 actttttcgt ctatatgggt ggcggactta tatttgcagt cttatttaga atgacaaagt
     1021 ccatttattg gcctattggg ctgcatatag tctacaacgg tattggtcag cttttgatgt
     1081 tactgtaatt ttggttgtta gtagtatcgc ggtctatttc tggggaaaga ttcccatttg
     1141 aacttaagaa ggagttgtga tgaaagtatt tcttcaaaat agagatttta ggcaattaac
     1201 tatcaaccag tggatttcaa cgcttgggga tacgattttt tatctggcct tcctgaatta
     1261 tgtggcagat gcgtcttttg cccctttggc gattttactt atcacgattt cagaaaccct
     1321 tccccaagtt ctacaaatct ttctgggagt tttggcggat tttcaacatc atcgtgtctt
     1381 gaaatacaca gtcattagtt ttacaaaatt tttgctttac tctatagtgt ccctatcgct
     1441 ttcagggcag tctttttcct tgttgctagt aacctttatt tgtctgctta acctcttgtc
     1501 tgacacattg agttattttt caggcgccat gctcactccg attttcatta gaattattgg
     1561 gcaagaccat ctggcagaag ctattggatt taaacagtca actgttagtt tagtgaaaac
     1621 aatcagtaat atcctaggag gagtcttact aggcattcta tccatccagt ttatttcctt
     1681 actgaacgct cttacctttt taatcgcatt tttaggtatc ctcttcataa aaactgacct
     1741 cttgaaagta gaaaaaacga ttagctatca agaaggactc tctgtaaaat ccttttgcca
     1801 gcatttgctc caatcatcga aattgatatg gaatatgaat aaggtgctct tggttttgtt
     1861 tattatctct actagtcaag cagtgataaa tgttacggtt cctatttcca ctctattttt
     1921 aaggaaccag ccctttttga atttacaaac tggtcaatct cttgccttgt tagccacact
     1981 agaattatca gcccttattt tgggaagtct tatgagtggc tatctgcaac ataccatttc
     2041 cataaaaaca gctctctatg cctcacttgt tatccagttg cttcttctag taggatttgc
     2101 cacagttcgt tttgactgga ttctcatctt tagtaccttg gatgcctttt tcgcgggtat
     2161 tctctctcct agattgcaag aactcgtctt taaacaaata cctgaggagt caatgggagc
     2221 agttcaatct tctatcggtg ccattacggt tgtcttacct agcttattta caatagcttt
     2281 ggtaaccatt gctaccagct ttggaactct ggcagttagc tttgttttat tgctatttct
     2341 tctagttgcc tttgtcatgc ttctgaatat tcgtgaaagt atttagtgga gaatttctat
     2401 aaaatatgtt tgagactgga ttgccagtct ttttatgatg gtcaaaatca acagactttt
     2461 ttctcttttc tgaatgtgtt atagtagact gaatctaaaa tagtacgaaa caattgctaa
     2521 aacattt
//
LOCUS       DAVMGZ010000164         2408 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000164 DAVMGZ010000000
VERSION     DAVMGZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.165"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            23..940
                     /gene="adcAII"
                     /locus_tag="VYQ93_001902"
     CDS             23..940
                     /gene="adcAII"
                     /locus_tag="VYQ93_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV3336539.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            948..>2408
                     /gene="phtD"
                     /locus_tag="VYQ93_001903"
     CDS             948..>2408
                     /gene="phtD"
                     /locus_tag="VYQ93_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEV3336540.1"
                     /translation="MKINKKYLAGSVAVLSLSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTVRGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRK
                     VGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKIDLPSSDREFY
                     NKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDV"
BASE COUNT          794 a          464 c          510 g          640 t
ORIGIN      
        1 ttaaatggtt aaggagttgt tcatgaagaa acaaaattta tttttagtcc tgttaagtgt
       61 ctttcttttg tgcttggggg cttgtggtca aaaggaaagt cagacaggaa aggggatgaa
      121 aattgtgacc agtttttatc ctatctacgc tatggttaag gaagtatctg gtgacttgaa
      181 tgatgttcgg atgattcagt caagtagtgg tattcactcc tttgaacctt cggcaaatga
      241 tatcgcagcc atctatgatg cagatgtctt tgtttaccat tctcatacac tcgaatcttg
      301 ggcaggaagt ctggatccaa atctaaaaaa atccaaagtg aaggtcttag aggcttctga
      361 gggaatgacc ttggaacgtg tccctggact agaggatgtg gaagcagggg atggagttga
      421 tgaaaaaacg ctctatgacc ctcacacatg gctagatcct gaaaaagctg gcgaagaagc
      481 tcagattatc gctgataaac tttcagaggt ggatagtgag cataaagaga cttatcaaaa
      541 aaatgcgcaa gcctttatca aaaaagctca ggaattgact aagaaattcc aaccaaaatt
      601 tgaaaaagcg actcagaaaa catttgtaac acaacataca gccttttctt atctagcgaa
      661 gagatttggg cttaatcaac ttggtattgc aggtatctct cctgaacaag aaccaagtcc
      721 acgacaacta acagaaattc aggaatttgt taagacctat aaggttaaaa cgatttttac
      781 agaaagtaac gcttcttcaa aagtagctga aactcttgtc aaatcaacag gtgtgggtct
      841 taaaactctg aatcctttag agtcagaccc acaaaatgac aagacctatt tagaaaatct
      901 tgaagaaaat atgagtattc tagcagaaga attaaagtga ggaaagaatg aaaatcaata
      961 aaaaatatct agcaggttca gtggcagtcc tttccctaag tgtttgttcc tatgaacttg
     1021 gtcgtcacca agctggtcag gttaagaaag agtctaatcg agtttcttat atagatggtg
     1081 atcaggctgg tcaaaaggca gaaaatttga caccagatga agtcagtaag agagagggga
     1141 tcaacgccga acaaattgtt atcaagatta cggatcaagg ttatgtgacc tctcatggag
     1201 accattatca ttactataat ggcaaggttc cttatgatgc catcatcagt gaagaacttc
     1261 tcatgaaaga tccgaattat cagttgaagg attcagacat tgtcaatgaa atcaagggtg
     1321 gctatgtgat taaggtagac ggaaaatact atgtttacct taaagatgcg gcccatgcgg
     1381 acaatattcg gacaaaagaa gagattaaac gtcagaagca ggaacgcagt cataatcaca
     1441 actcaagagc agataatgct gttgctgcag ccagagccca aggacgttat acaacggatg
     1501 atgggtatat cttcaatgct tctgatatca ttgaagatac tggtgatgct tatatcgttc
     1561 ctcacggcga ccattaccat tacattccta agagtgattt gtctgccagt gaattagctg
     1621 ctgcccaagc ctattggaat gggaagcagg gatctcgtcc ttcttcaagt tctagtcata
     1681 atgcaaatcc agctcaacca agattgtcag agaaccacaa tctgactgtc actccaactt
     1741 atcatcaaaa tcaaggggaa aacatttcaa gccttttacg tgaattgtat gctaaaccct
     1801 tatcagaacg ccatgtagaa tctgatggcc ttattttcga cccagcgcaa atcacaagtc
     1861 gaaccgtcag aggtgtagct gtccctcatg gtaaccatta ccactttatc ccttatgaac
     1921 aaatgtctga attggaaaaa cgaattgctc gtattattcc ccttcgttat cgttcaaacc
     1981 attgggtacc agattcaaga ccagaacaac caagtccaca atcgactccg gaacctagtc
     2041 caagtccgca acctgcacca aatcctcaac cagctccaag caatccaatt gatgagaaat
     2101 tggtcaaaga agctgttcga aaagtaggcg atggttatgt ctttgaggag aatggagttt
     2161 ctcgttatat cccagccaag gatctttcag cagaaacagc agcaggcatt gatagcaaac
     2221 tggccaagca ggaaagttta tctcataagc taggagctaa gaaaattgac ctcccatcta
     2281 gtgatcgaga attttacaat aaggcttatg acttactagc aagaattcac caagatttac
     2341 ttgataataa aggtcgacaa gttgattttg aggctttgga taacctgttg gaacgactca
     2401 aggatgtc
//
LOCUS       DAVMGZ010000165         2363 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000165 DAVMGZ010000000
VERSION     DAVMGZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.166"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..410
                     /locus_tag="VYQ93_001904"
     CDS             <1..410
                     /locus_tag="VYQ93_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3336541.1"
                     /translation="YGRSLKGQLRRGKVSGRRYQRISLVAGLTNGELIAPMTYEEMMT
                     SDFFEVWFQKFFLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNP
                     IEKTWAHIKKHLKKVLPSCNTFYEALLSCSCFN"
     gene            complement(403..570)
                     /locus_tag="VYQ93_001905"
     CDS             complement(403..570)
                     /locus_tag="VYQ93_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV3336542.1"
                     /translation="MKKRILSAGLTFTSALLLAACDQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(735..2342)
                     /locus_tag="VYQ93_001906"
     CDS             complement(735..2342)
                     /locus_tag="VYQ93_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV3336543.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELEVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPMQEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
BASE COUNT          670 a          496 c          448 g          749 t
ORIGIN      
        1 aatatggtcg ctcattaaaa ggtcagttaa gaagaggcaa agtatctgga agaagatatc
       61 agaggatttc tttggttgca ggtctaacaa atggtgaatt aatcgctcca atgacttacg
      121 aagagatgat gacgagcgac ttttttgaag tatggtttca gaagtttttc ttaccaacat
      181 taaccacacc atcggttatt attatggata atgcaagatt ccatagaatg gggaagctag
      241 aacttttatg cgaagagttt gggcataaac ttttacctct tcctccctac tcacctgagt
      301 acaatcctat tgagaaaaca tgggctcata tcaaaaagca cctcaaaaag gtattaccaa
      361 gttgcaatac cttttatgag gctcttttgt cttgttcttg tttcaattga ctatattatc
      421 agcgtaatag tctaatagat agttaaatgt agttggattt ccactaaagg ttgatgagta
      481 agtttttgta tctgaacctg attggtcgca agcagctaaa agcaaagcag atgtaaaagt
      541 caggcctgca ctaaggatac gcttctttat gttcgtcttc tttctcctta atagtgggaa
      601 tttgtaaagt taattgaatt tcgagaatga aggtttataa accttggtta taaaaaacaa
      661 aggatttctg ccttttatac agtcctccct tgtttttata cgatttcaat tttaaatttt
      721 tctgcaaaaa atatttatag taattccaca cagaaagcat cccatggaac taagatttgt
      781 ttttcaaaga cttcttgagc tagggtgttt tcaatcaaga cagatttgac ttttccttct
      841 actgtcaagt cttgctcttc attggacaag ttagccacaa ctaggaagcg acggtcgcca
      901 tccttacgta tataagcaaa gaccttatca gccgtatcaa gcaattcaaa gtcagctcga
      961 attagccagc tattctcctt gcgaatttgg accagtttct gataggtata gaaaatagaa
     1021 tctggatttg ccagcgcttc ttggacgttg atcatctcgt aatttggatt aactgccaac
     1081 caaggttgac ctgttgagaa accagcgttt ttgctctcgt cccattgcat aggggtacgg
     1141 gcattgtcac gtccaataac acggatactg tccatgattt cttgcatcgg aacacctttt
     1201 tcaagagcct cacgcgcata gttgagagat tcaatatctt ctacttgatc tagtgtttca
     1261 aacggatagt tggtcatccc aatctcctca ccttggtaga tataaggagt tcctctcatg
     1321 agatgaagta agattgcaaa ggctttggca gatttttcgc ggtattcttg gtcatttccc
     1381 cagattgaga caatacgagg gaggtcatgg ttgttccaga agagggaatt ccagccgtcc
     1441 tcaacttcta actctgtctg ccatttgttg aagatttctt ttaacttagc gatattcagc
     1501 tctttttgat agtgccattt aggctgacct tcctgatact gaagaccgat atgttcaaac
     1561 tggaagacca tagacaattc ttgccccttt ggatcagagt agagcttggc aatctctggc
     1621 gttgctcccc aagtctcccc tactgtcaag agatccttat ctccaaaggt cgcctgattc
     1681 atttccttga gatagggatg gagcatagga ccattattga ctaccttctc gtcaggaatt
     1741 ttgccaatca tgtcaataac atccatacgg aaaccaccaa tacctttatc aatccagaag
     1801 ttcatcatct cataaatttt ctggcgaagt ttttcatttt cccagttgag atccggctgt
     1861 tttttgctga aaaagtggag atagtattga cctgactttt catcgtattc ccaagcagac
     1921 ccactaaaga tagaatctag gtcattgggt tcatcgcgcc agatatagta gtctcgctca
     1981 gggctgtcag tattttcaca ggcttcgaca aaccaagcat gttcatctga ggtatgatta
     2041 accaccaagt ccatgatgat acgaatgtca cgcttcttag cttccgcaat cagttgatcc
     2101 atatcctcca tggttccaaa aatcgttgca atcgcttgat aatcagcaat atcgtagcca
     2161 ttatcatcca tagggctgtc ataaacggga gaaagccaaa tcgctgtgat tcctagctta
     2221 gctagatagt ccaacttact ggtaatacct ggcaaatcac caactccatc tccattacta
     2281 tccataaaac tctttggata gacttgatag actacggcat tatgccacca tttttcttgc
     2341 atcttcttac ctcattattt taa
//
LOCUS       DAVMGZ010000166         2304 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000166 DAVMGZ010000000
VERSION     DAVMGZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.167"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            59..1168
                     /locus_tag="VYQ93_001907"
     CDS             59..1168
                     /locus_tag="VYQ93_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV3336544.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            1325..2215
                     /locus_tag="VYQ93_001908"
     CDS             1325..2215
                     /locus_tag="VYQ93_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV3336545.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
BASE COUNT          788 a          314 c          496 g          706 t
ORIGIN      
        1 agatctgaaa aagaattaaa gctgaaattt gaaaatatat aaaaagagga taaaaattat
       61 ggtaaattac ggtattgttg gagctggata ttttggagct gatttagctc gctcaatgaa
      121 caaaattgaa gatgcaaaag tggttgcggt atttgaccca aatcatggag aagaagttgc
      181 tcaagagttg ggatcagatg tttgtgcaag tttagatgaa cttgtagcac gtgaagatat
      241 tgattgtgtg atcgtagctt cacctagcta ccttcaccgt gaaccagttg tgaaagctgc
      301 tcaacatggc aaacacgtat tttgtgaaaa gccaattgca ttgtcttatg aagattgtaa
      361 agccatggtt gacgcatgta aagaaaataa tgtcatcttt atggctggtc acatcatgaa
      421 cttctttaac ggtgtacacc atgctaaaga attgattact caaggtaaaa tcggtaaagt
      481 tctttattgc catgctgctc gtacaggttg ggaagaacaa caaccaactg tatcatggaa
      541 gaaacttcgt tctcaatctg gaggacattt gtaccaccat attcatgaat tagattgcat
      601 tcagtttatc atgggaggac ttcctgaaaa agcgacaatg gtaggaggca atgtatatca
      661 taaaggtgaa aactttggtg atgaagatga tatgctcatt gtaaacttag aatactctga
      721 tgatcgttat gctgttttgg aatatggtaa tgctttccgt tggggtgaac actacgtctt
      781 gattcaagga actgaaggag ctatcaaact tgacttgttc aatactggcg gtactcttcg
      841 tgttaaaggt gaaggagaat cacacttctt agttcatgaa actcaagagg aagatgatga
      901 tcgtacagct atctataccg gtcgtggtat ggatggagca attgcgtacg gtaaaccagg
      961 agtacgttgc ccattatggt tgcaaacatg tattgataaa gaaatggaat atctacatga
     1021 catcattaaa ggtggagaaa ttacagaaga atttgaaaaa cttctcaatg gtgtagctgc
     1081 tttagaatca atcgctaccg ctgatgcatg tactttatca gttaaagaag atcgaaaagt
     1141 aagtctttca gaaatcacaa atgcttaact tttgtaaaac agaatagtaa attcttgtca
     1201 ttatataatt tctaaagttc tgtgatacaa ctcattgaat aaagaaatag agatgggact
     1261 gggataatgc ccagtcccat tttttatcaa aaagtaatga gatcaaaaat gtgggagtgt
     1321 tgaaatgaag attataggta tcgatattgg cggaacaaca attaaggcag atttatacga
     1381 tgagtttgga acgagtttga atcatttcaa agagatagaa acaattattg actatgattt
     1441 gggaacgaat cagatattaa atcaggtctg tgatttaatt ggtgagtata ctttaaatca
     1501 ttcaattgat ggtgttggga tttccactgc tggagttgtt aatgctaata ctggagaaat
     1561 catctatgca ggctatacaa taccagggta tatcggagta aactttactg ccgaaataga
     1621 aaaacgtttt gggttgtata cttttgttga aaatgatgtt aattgtgctg cattaggtga
     1681 attgtggaag ggacaagcca aagataagaa aaatgtagta atggttacta ttggaacagg
     1741 tataggaggc agtattattg tcaacggaca aattgttaac ggatttaact atactgctgg
     1801 tgaagtaggt tatattcctg taggtaattc ggattggcaa agtaaagcct caacaaccgc
     1861 attgattcat ttatatcaaa aaaagagctt gaaaactaat caaactggac gtactttctt
     1921 cactgattta agatctggag ataaagttgc tgaagaaact tttgaaattt ttgtagaaaa
     1981 tctaacaaaa ggtttattaa cgatttctta tctacttaat ccagaaattc tcatattagg
     2041 aggtgggatt ctggatagta aggatatttt gttacctgaa attcaaagtt ctttagctaa
     2101 aaatgcaatg gataataggt ttttacctaa aaatcttgtg gcagctacat taggaaatga
     2161 agctggtcgt ataggagctg taaaaaattt cttagataga atttctaata aatagtatgt
     2221 aagataagga ggtgtcacaa tgactaactc tgtattttcg acaatgcaag atattgagaa
     2281 tgttgcaacc gatattataa aatc
//
LOCUS       DAVMGZ010000167         2219 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000167 DAVMGZ010000000
VERSION     DAVMGZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.168"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            107..1591
                     /locus_tag="VYQ93_001909"
     CDS             107..1591
                     /locus_tag="VYQ93_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV3336546.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            1686..2168
                     /locus_tag="VYQ93_001910"
     CDS             1686..2168
                     /locus_tag="VYQ93_001910"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV3336547.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT          665 a          529 c          438 g          587 t
ORIGIN      
        1 tttaaaattt tgtgcaagcg tttgcttaat ctttttaatt tcttttttag ctccgtagtt
       61 tcctaacttc caatttttta ttataataga gtccagaggt aaaaaaatga aaaaacaagc
      121 ttttagttct gaacaatatt tgaatttaca acgcgaccat attttggagc gcattaacca
      181 atttgacggc aagctctact tagagtttgg cggtaaaatg ttagaagatt tccacgctgc
      241 tcgtgtcctt cctggttatg aacctgacaa caaaatcaag ctcttgcaag aattgaaaga
      301 gcaggttgag gttgtgattg ccattaatgc tagcaacatt gaacattcca aagcacgtgg
      361 cgacttaggc atttcttatg accaagaagt tcttcgtttg attgacaaat tcaatgaatt
      421 agggattttt gttggttccg ttgtcattac acaatacgct ggccaacccg ctgcagatgc
      481 cttccgcaat caacttgaga aaaacggaat tgattcttat cttcattatc caatcaaagg
      541 atatccgacg gatatggatc acatcatttc cccagaaggc atgggcaaaa acgactacat
      601 caaaaccagt cgcaacttga ttgtcgtaac cgctcctgga cccggttctg gaaaattggc
      661 aacgtgtatg tccaatatgt accacgacca aatcaatggc atcaaatctg gctacgctaa
      721 atttgaaacc ttccctgttt ggaatcttcc ccttcatcat ccagttaact tggcttatga
      781 agctgccaca gctgaccttg atgatgtcaa catgattgac cccttccatc ttcaaaccta
      841 tggagaaacc actgtcaact acaaccgtga tatcgaaatc ttcccagtgc tcaagcgcat
      901 gttggaacgt attctcggaa aatcaccata cgcttcaccg acagatatgg gtgtcaacat
      961 ggttggtttc gctattacag atgacgaggc tgctgtcgaa gcttctaaac aagaaatcat
     1021 ccgccgttac tatcaaacag ttcttgactt caaagctgaa aaagttggcg aagctgccgt
     1081 caagaaaatt gagttgctca tgaacgacct cggtatcaca cctgcagacc gtaaggttgc
     1141 tgtcgttgcg cgccaaaaag cagaagaaac tggtggacca gccctagcct ttgaattgcc
     1201 aaatggggaa atcgtcactg gtaagaactc agaactcttt ggccctacag ccgctgcctt
     1261 gatcaacgcc atcaaaaaat cagctgacat cgctaaagaa gtaaaattaa tcgagccgga
     1321 agttgttaag ccaatccaag gtcttaaaat cgatcatctc ggtagccgca atccacgcct
     1381 tcattcaaat gaaatcctga ttgcacttgc tatcacagct acagaaaatc ctgatgctgc
     1441 ccgcgctatg gaagaactcg gcaacctcaa aggaagcgaa gcccactcaa ccatcatctt
     1501 gactgatgaa gacaagaatg tccttcgtaa actgggtatc aacgtaacct ttgacccata
     1561 ctaccaatac gaccgcttat atcgtaagta aagatttcaa cctctccagc ctcggagagg
     1621 ttttctctct gtctcaggag caacctttat gatataatga gagaagaaaa ctgaaaggat
     1681 ttaccatgtc aaaagaagtt attgtcgaaa gttttgaact tgaccacacc attgtcaaag
     1741 caccctatgt tcgcttgatt ggggaagaaa caggaccaaa aggagacatc atctccaatt
     1801 atgatattcg cttggtgcaa ccaaacgaag actctatccc tactgccggc cttcacacta
     1861 tcgagcacct cttagccaaa ctcatccgta cccgcattga cggcatgatt gactgttcac
     1921 catttggttg ccgcacaggc ttccacatga ttatgtgggg acgtcacacc agtgctaaaa
     1981 tcgcagctgt tatcaaggat tcgctcaagg aaatcgctga aactactact tgggaagatg
     2041 tcccagggac aaccatcgaa tcttgcggaa actacaagga ccacagcctc ttctctgcta
     2101 aagaatgggc aaaactcatc ttggaacaag ggatttcaga tgatgccttt gaacgtcatg
     2161 tgatttaaaa tcgagtaggg gataatctct agcccctctc acaccaccgt acgtgccgt
//
LOCUS       DAVMGZ010000168         2203 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000168 DAVMGZ010000000
VERSION     DAVMGZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.169"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            61..2043
                     /locus_tag="VYQ93_001911"
     CDS             61..2043
                     /locus_tag="VYQ93_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3336548.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKAEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAVVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGGITDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT          735 a          394 c          460 g          614 t
ORIGIN      
        1 tatattgaca tctctctgaa aagagtctat aatagagaga aagttttaaa ggagaagatg
       61 atgaaaagtt caaaactatt tgcccttgcg ggtgtaacct tattggcggc gactacttta
      121 gctgcatgct ctggatcagg ttcaagcact aaagctgaga agacattctc atacatttat
      181 gagacagacc ctgataacct caactatttg acaactgcta aggctgcgac agcaaatatt
      241 accagtaacg tggttgatgg tttgctagaa aatgatcgct acgggaactt tgtgccgtct
      301 atggctgagg attggtctgt atccaaggat ggattgactt acacttatac tatccgtaag
      361 gatgcaaaat ggtatacttc tgaaggtgaa gaatacgcgg tagtcaaagc tcaagacttt
      421 gtaacaggac taaaatatgc tgctgataaa aaatcagatg ctctttacct tgttcaagaa
      481 tcaatcaaag ggttggatgc ctatgtaaaa gggggaatca cagatttctc acaagtagga
      541 attaaggctc tggatgatca gacagttcag tacactttga acaaaccaga aagcttctgg
      601 aattctaaga caaccatggg tgtgcttgcg ccagttaatg aagagttttt gaattcaaaa
      661 ggggatgatt ttgccaaagc tacggatcca agtagtctct tgtataacgg tccttatttg
      721 ttgaaatcca ttgtgactaa atcttctgtt gaatttgcga aaaatccgaa ctactgggat
      781 aaggacaatg tgcatgttga caaagttaaa ttgtcattct gggatggtca agataccagc
      841 aaacctgcag aaaactttaa agatggtagc cttacagcag ctcgtctcta tccaacaagt
      901 gcaagtttcg cagagcttga gaagagtatg aaggacaata ttgtctatac tcaacaagac
      961 tctattacgt atctagttgg tacaaatatt gaccgtcagt cctataaata cacatctaag
     1021 accagcgacg aacaaaaggc atcgactaaa aaggctctct taaacaagga tttccgtcag
     1081 gctattgcct ttggttttga tcgtacagcc tatgcctctc agttaaatgg acaaactgga
     1141 gcaagcaaaa tcttacgtaa tatctttgtt ccaccaacat ttgttcaagc agatggtaaa
     1201 aactttggcg atatggtcaa agagaaattg gtcacttatg gggatgaatg gaaggatgtt
     1261 aatcttgcag attctcagga tggtctttac aatccagaaa aagccaaggc tgaatttgct
     1321 aaagctaaat cagccttaca agcagaaggt gtgactttcc caattcattt ggatatgcca
     1381 gttgaccaga cagcaactac aaaagttcag cgcgtccaat ctatgaaaca atccttggaa
     1441 gcaactttag gagctgataa tgtcattatt gatatccaac aactacaaaa agacgaagta
     1501 aacaatatta catattttgc tgaaaatgct gctggcgaag actgggattt atcagataat
     1561 gtcggttggg gtccagactt tgccgatcca tcaacctacc ttgatatcat caaaccatct
     1621 gtaggagaaa gtactaaaac atatttaggg tttgactcag gggaagataa tgtagctgct
     1681 aaaaaagtag gtctatatga ctacgaaaaa ttggttactg aggctggtga tgaggctaca
     1741 gatgttgcta aacgctatga taaatacgct gcagcccaag cttggttgac agatagtgct
     1801 ttgattattc caactacatc tcgtacaggg cgtccaatct tgtctaagat ggtaccattt
     1861 acaataccat ttgcattgtc aggaaataaa ggcacaagtg aaccagtctt gtataaatac
     1921 ttggaacttc aagacaaggc agtcactgta gatgaatacc aaaaagctca ggaaaaatgg
     1981 atgaaagaaa aagaagagtc taataaaaag gctcaagaag atctcgcaaa acatgtgaaa
     2041 taactgttgc aaaatataag aaaggattta gtatttctct tgaatgctga atcctttttt
     2101 acatttgtaa agaaagattc taaatgtact gccccccaaa agttggagcc tctttttgtc
     2161 agaatagaga aaatttttgt taattttact tgtttcctat tgc
//
LOCUS       DAVMGZ010000169         2186 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000169 DAVMGZ010000000
VERSION     DAVMGZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.170"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            86..2044
                     /locus_tag="VYQ93_001912"
     CDS             86..2044
                     /locus_tag="VYQ93_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3336549.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKSKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGIESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
BASE COUNT          741 a          366 c          451 g          628 t
ORIGIN      
        1 tttttcgatt ttttggaaaa ataattgact aaacaaacca aacgttgtat aataaaacaa
       61 atatttaaag caggaggttc tggaaatgaa aaagtctaag agcaaatatc taaccttggc
      121 aggtcttgtc ctgggtacag gagttttatt gagcgcgtgt ggaaattcta gcacggcgtc
      181 aaaaacctac aactatgttt attcaagtga tccatctagc ttgaactatc tagcagaaaa
      241 ccgcgcagca acatccgata ttgttgcaaa tttggtagac gggttattag aaaatgacca
      301 atatgggaat attattccat cattagcaga ggattggact gtttctcagg acggtttgac
      361 ctatacctac aaacttcgta aggatgccaa gtggtttact tctgagggag aagaatatgc
      421 gcctgtaact gcccaggatt ttgtgacagg tttgcaatat gcagctgata aaaaatcaga
      481 agccttgtat ctagtgcagg actctgttgc tggtttggat gactatatca ctggtaaaac
      541 aagcgacttt tcaactgtcg gtgtcaaggc acttgatgac caaacggttc aatatacttt
      601 ggttaaacca gaactttact ggaattcaaa aacacttgca acgatacttt ttcctgttaa
      661 tgcagatttc ctgaaatcaa aaggggatga ttttgggaag gcggatccat ctagtatttt
      721 gtacaatgga cctttcttga tgaaagcact tgtctcaaaa tctgctattg aatataagaa
      781 aaaccctaat tactgggatg ctaagaatgt ctttgtagac gatgtgaaat tgacctacta
      841 tgatggtagc gaccaagaat cactggaacg taattttaca gctggtgctt atactacggc
      901 tcgtcttttt cctaacagct ccagctatga agggattaaa gaaaaataca aaaacaatat
      961 catatatagt atgcaaaatt caacttcata tttctttaat tttaacctag ataggaagtc
     1021 ttacaattat acttctaaaa caagtgacat tgaaaagaaa tcgactcagg aagcagttct
     1081 caataaaaac ttccgtcagg ctatcaattt tgcttttgac agaacatctt atggggctca
     1141 gtctgaaggg aaagaaggtg caacaaagat tttgcgtaac ctagtggttc ctccaaactt
     1201 tgtcagtatc aagggaaaag actttggtga agttgtagcc tctaagatgg tcaactatgg
     1261 taaggaatgg caaggtatca actttgcgga tggtcaagac ccttactaca atcctgagaa
     1321 atccaaggct aagtttgcgg aagctaagaa agaactcgaa gcaaagggtg ttcaattccc
     1381 aatccacttg gataagactg tggaagtaac agataaagta ggcatacaag gagttagttc
     1441 tatcaaacaa tcaattgaat ctgttttagg ttctgataat gtagtgattg acattcagca
     1501 attaacatca gatgagtttg acagttcagg ctactttgct caaacagctg ctcagaaaga
     1561 ctatgattta tatcatggcg gttggggacc tgattatcaa gacccgtcaa cctatctcga
     1621 tatttttaat actaatagtg gaggagttct acaaaatctt ggactagagc ctggagaagc
     1681 caatgacaag gctaaggaag ttggactgga tgtctatact caaatgttgg aagaagctaa
     1741 taaagagcaa gatccagcca aacgttatga gaaatatgct gatattcaag cttggttgat
     1801 tgatagttct ttagttcttc caagtgtttc gcgtggggga acaccatcat tgagaagaac
     1861 cgtaccattt gctgctgcct atggtttaac cggtacaaaa gggattgaat catataaata
     1921 cctcaaagta caagataaga ttgtcacaac agacgaatat gcaaaagcta gagaaaaatg
     1981 gttgaaagaa aaagaagaat ccaataaaaa agcccaagaa gaattggcaa aacatgtcaa
     2041 ataaaatcta attgtagata agtttgtgta agaagtcttt gtgcttaaaa acgcataata
     2101 tcaggtatat aaaatcctag atgttatgcg ttttgttata ggaagattta ttagatttta
     2161 tcctcaaatt gagttattcc cgactt
//
LOCUS       DAVMGZ010000170         2181 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000170 DAVMGZ010000000
VERSION     DAVMGZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.171"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            266..1669
                     /locus_tag="VYQ93_001913"
     CDS             266..1669
                     /locus_tag="VYQ93_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV3336550.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGP
                     YILSDKRWDEAPIYLRYGVFRPSRDDEKKVVIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSEMPKIYWYGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKAFLLVN"
     gene            1858..>2181
                     /locus_tag="VYQ93_001914"
     CDS             1858..>2181
                     /locus_tag="VYQ93_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336551.1"
                     /translation="MKDVVYSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLIN
                     GSLKPQKGYVNLLSHGKKSSDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVL
                     KKVNLY"
BASE COUNT          788 a          278 c          425 g          690 t
ORIGIN      
        1 gattctctgg agttatggat ctcgttccaa tatgtgcacg ttggaatgtc agtgcttata
       61 tggggggaat ccaaaggatt ctcccttttt attttaataa gtctgtctag ctatagatat
      121 attaagtttg atagaatttc ttggagattt tgttatggat tataatttca atttggagca
      181 tcctttcttt ttcactaata atgattatag tacagatact agcataaagt actaagtaag
      241 tctgcctttt aattggcacg aagtaatgaa taatgatgag tgggtttatc aataccctat
      301 tggaaaattt gttgaaagac aagggtggaa aatacacatt agttcagagt ataatagttc
      361 acacgaattg ttacaagatg tagcaaaaat ttgccatgaa atgcgaattc catttaaaca
      421 tcttagtaca gaagacaaat ttatcatgcg aaacgggaaa ttggttagtc gaggattttc
      481 tggtaaattt attacatgtt atccaaatca aaatgaatta gagtcggttt tgcaaaggtt
      541 agaaagtgcc ttaaagcaat ataatgggcc atatatttta agcgataaac gttgggatga
      601 agcaccaata tatctgaggt atggtgtgtt taggccgtca agggatgatg aaaagaaggt
      661 tgttattgat gaattaattg ttggcgatga agttgttaaa gatgaacgtt taccagtatt
      721 taaaattcca aaaggtatag tgccccctga ctttttgaat aaatggcttg acaagaaaga
      781 taaaaaacag ggagactttc cctttattat agataatgca attagatttt caaacagtgg
      841 tgggatatat aatgctagac taaaagaaga tgggaaaaaa ataattttaa aagaggctag
      901 accgtataca ggtctaggat ttgatggtac ttattcttca gagagattag cttcggagtg
      961 caaagcacta aaaattctaa atgaatggag tgagatgcct aaaatatact ggtacggtaa
     1021 gatctgggag cacaccttct taggaattga acatatgaag ggcgttccac taaatagatg
     1081 ggttaccaat aattttcctt tatatgaagt tgttgataag accaaagatt atctattgag
     1141 agtttcaaag atagtagaaa aactcatcga tttaacgaat aagtttcatt ctgaaaatgt
     1201 atatcatcaa gatttacatt taggaaatat cttggttaag gatgaagatg agattagtat
     1261 aatagactgg gaacaggctg tcttttcgaa tgatgaaaaa gttgtacata aagttgcagc
     1321 ccctggtttt agagcatggc gagaaacact tcccagtgag atagattggt atggtattag
     1381 acaaatagct cattatttat acatgccact agtaactaca tcggatttga catacaacta
     1441 tgtaagtcaa accaggatag aaggaaaaaa actatttgag tctttgggtt atacaagaga
     1501 acatatagat tatgtagaaa gtttattgtc atatctagat tctaaatgtc cacagattga
     1561 gaatatttcc aggaaaaaag ttttaaaacc catgcatgag attagaacta tcgagagtga
     1621 gcaagatata caagatttta ttataaaagc ttttctttta gtgaactgat aggtattttc
     1681 ttagcaaacg ataaagtttt agggccgata caatcaattg cctattcatt aaataagata
     1741 aatacaacca aagatttaag gaaaccgttt ttaaaatact taagtggaga gaagaatttt
     1801 gtagacgctg aacatgataa taacggactg tatacttcat caatagatga gatacacatg
     1861 aaagatgttg tatattctat tacaccagaa aataaattaa gtattgactt ctcatttaag
     1921 tcaccattta gggtattatt aacaggaact tctggcagtg ggaaaacaac gattttaaat
     1981 ttaattaatg gttctttaaa gccacaaaaa ggttatgtaa atttgttatc acatgggaaa
     2041 aagagttcag attcaatacc aacagttgat cagacaccat atatttttga cactactatt
     2101 cgtgagaacg taactttatt tcaaaatgaa tatttttcag atgatcagat aattgaggtg
     2161 ttaaaaaagg taaatctata t
//
LOCUS       DAVMGZ010000171         2166 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000171 DAVMGZ010000000
VERSION     DAVMGZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.172"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(227..2146)
                     /locus_tag="VYQ93_001915"
                     /pseudo
     CDS             complement(227..2146)
                     /locus_tag="VYQ93_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
BASE COUNT          521 a          470 c          364 g          811 t
ORIGIN      
        1 atgttccttt ttgtccatca ggagtcaccc actcatatac gtttttagat ttatcacctc
       61 taaattcgcc agtagtatta tcagtagaat tattaatgaa attatgtgct actgttaaaa
      121 gtacatttgg tgcaataaaa gtagctgatc ctactccagt aaccttcgac gaatctgtaa
      181 taaagttcat attaattaat gcactagatt ttttctgaat actcatttct tctggtgtat
      241 tagtattttc attatttggt ttagtatctt ctgcactagg ttgttcgatt ttaccagtat
      301 attctttcag tgcttctact ttctctggct cgactatcgc tccggcttgc acccctgtgt
      361 attctggtag tggtgctatc tgctctggtt caacaatagc gccagcctgc acccctgtgt
      421 attcttttga tggttctact ttttctggtt ctactattgc tccggcttgc actcctgtgt
      481 attccttcgg tgcttctacc ttctctggtt cgactatcgc tccagcctgc actcctgtgt
      541 attccttcgg tgcttctact ttctctggct cgactatcgc tccggcttgc acccctgtgt
      601 attctggtag tggtgctacc tgctctggtt caacaatagc gccagcttgt actccagcat
      661 attccggttc aactttttca ggttcaacaa tagctccagc ttgtactcct gtatattctg
      721 gtagcggttc tacctgctct ggatcaccct tgtcagtcat gactgcctct ggtaacgctg
      781 gttgaacttc tggtacgcct ttatcactta ctacagcttc tggcaactct ggttggactg
      841 ctgactcacc tttgtcggtt aatacagctg ctggtagttc aggctgaatt gcgggttcaa
      901 caatagctcc agactgtact tccttatgtt ctgcaccagt ctcagcttgt tcctttataa
      961 cttgagcttt tttagtacct ttttcgacta ttcttgaaac aggcgcagtc gttgaagttg
     1021 aaataatttc tcgtgaaact tcttcattgt ttacagaaaa tattctgacg atttcaactt
     1081 tcttacctaa tgcaccttct tgttttactc ttacagttcc ttctgctaaa tcaggatttt
     1141 cttgaatttc ttcttgaaaa tctatttttg tttctatagt ttcgtcacga tataagagtt
     1201 ccggtttgtt caactgacct gatagcactt catcctgtgg atttaatgta tttaccccag
     1261 tcttttcctt tggagaaatc ttctcctctt tcttcgtttc tagattctta tgttcggcta
     1321 attgttcttg agaatctgaa gattgtttct cttcttttct tggattgatt aattcagtag
     1381 agaaaggttt ttcaactact tgaacttctg tcggcttagt tgaagaaaca ggtgtttgtt
     1441 cctgaatagc ttgtactgtt gatggatggt ctacaaaatt tggtgtaaca ttataatcca
     1501 ccttttgttg ttttgtagga gtggctgctg atttttcttg attacttact tcaaaatcag
     1561 aagtcgtttt tccctctttg atatatccaa tataagtgta acctgaaatc tctttaggaa
     1621 gaggtaattt ttctccagag gtcaattcat agtccgtatt gtaatttagc aaaagatgat
     1681 tttctaaagc atggactgaa actaagacac catttcccat tcccgcaacc aatactaaat
     1741 gtaataccgt tttattctta acctttttct tggaaacagc aaaaattaaa attcccagag
     1801 cagctaagct agcaccagca actagggctt gcctctcatt cttgcttcca gtatttggta
     1861 attccgccag ttgattttga gaatttaact tataaacaag ataataagtt tcatcatcat
     1921 tctccacgta tgtcggaata tcatagacaa gctgcttctt ttcttctgat gatagctctg
     1981 aatctgccac atatttatag tgaactcccg cagtttcttg agcatccaca gatgaactag
     2041 ctaatacaga cataaaaaat aaacttgaaa tcgttgcaga tacaagtcct actgataatt
     2101 ttctaaatga aaaacgctct tgtttttcac caaaatactt ttccattatt cctccttgaa
     2161 ataaaa
//
LOCUS       DAVMGZ010000172         2157 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000172 DAVMGZ010000000
VERSION     DAVMGZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.173"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..134
                     /locus_tag="VYQ93_001916"
     CDS             <1..134
                     /locus_tag="VYQ93_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV3336552.1"
                     /translation="SEESLVEWLYDWISTNRYELPKEFSIRMAHKYHESVTEIFGDE"
     gene            184..1434
                     /gene="ilvA"
                     /locus_tag="VYQ93_001917"
     CDS             184..1434
                     /gene="ilvA"
                     /locus_tag="VYQ93_001917"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV3336553.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT          652 a          338 c          469 g          698 t
ORIGIN      
        1 atagtgaaga aagtttggta gaatggttat atgattggat ttccacaaat cgttatgaac
       61 ttcctaaaga gttttcgatt cgtatggctc ataaatacca tgaaagtgtt actgaaattt
      121 tcggagatga ataactaaaa aacagtcatt agtgactgtt ttttatagaa aaagaggttt
      181 tatatgttaa gttcaaaaga tataatcaag gctcacaagg tcttgaacgg tgtggttgtg
      241 aatactccac tggattacga tcattattta tcggagaagt atggtgctaa gatttatttg
      301 aaaaaagaaa atgcccagcg tgttcgctcc tttaaaattc gtggtgccta ttatgccatt
      361 tcccagctca gcaaggaaga acgtgaacgt ggggtagttt gcgcttctgc gggaaatcat
      421 gcgcagggag tagcctatac ttgtaatgaa atgaaaattc ctgctactat ctttatgccc
      481 attactacgc cacaacaaaa gattggtcag gttcgctttt ttggtgggga ttttgtaact
      541 attaaactag ttggagatac ctttgatgcc tcagccaaag cagctcaaga atttacagtc
      601 tctgaaaatc gtacctttat tgatcctttt gatgatgctc atgttcaagc aggtcaagga
      661 acagttgctt atgagatttt agaagaagct cgaaaagaat cgattgattt tgatgctgtc
      721 ttggttcctg ttggtggtgg cggtctcatt gccggggttt ctacctatat caaggaaaca
      781 agtccagaga ttgaggttat cggagtagag gcgaatggag cgcgttccat gaaagctgcc
      841 tttgaggctg gaggtccagt caaactcaag gaaattgata aatttgcgga tgggattgct
      901 gtgcaaaagg taggtcagtt gacctatgaa gcaactcgtc aacatattca aactttggta
      961 ggtgtcgatg agggattgat ttctgaaacc ttgattgacc tttactctaa gcaagggata
     1021 gtcgcagaac ctgctggagc agctagtatc gcctctttag aggttttagc tgaatatatt
     1081 aaggggaaaa ccatttgttg tatcatttct ggaggaaata atgatatcaa ccgtatgcca
     1141 gaaatggaag agcgtgcctt gatttatgat ggtatcaaac attattttgt ggtcaatttc
     1201 ccacaacgtc caggagcttt gcgtgagttt gtaaatgata tcctggggcc aaatgatgat
     1261 atcacacgtt ttgagtatat caaacgagct agcaagggaa caggcccagt attgattggg
     1321 atcgctttag cagataagca tgattatgca ggtttgattc gtagaatgga aggttttgat
     1381 ccagcttata ttaacttaaa tggtaatgaa acgctttata atatgcttgt ctgaggacta
     1441 ataaaaaaat atcatattat ttgtacaact gatgtatata tgctataatg aagatgaaga
     1501 aaggggacga ttcctatggt tttagaaaac ttatcgtgca attcttactt ctgttcatta
     1561 tagcacttat gatgtttggc tcagatttcc atccttcatt ttgatttcct atctattgac
     1621 aagcatagtc acactgtctt taatactctt cgaaaatctc ttcaaaccac gttagctcta
     1681 tctgcaacct caaaacagtg ttttgagcaa cttgcggcta gcttcctagt ttgctctttg
     1741 attttcattg agtataaggt atgatttgat ttctttttgt tgacaaatat accatattaa
     1801 aaagatatat aagtaattaa ctgagcttat ctgtcttgtc atctctatta aggatggttt
     1861 agataatcgg gtgtctgctt ctaggctagc acctcaatat ccaaaggagt gatgaatttg
     1921 aaggatataa gaaataccta tctctcagat gatttattga ggaagaaaga taggagtttt
     1981 tgagctagtg aaggcttgga tttctaaagg ttagaactat catcttcagt tcttaaatcg
     2041 aagaaataag ctatcttacg gaaatagaga agcatttttt aagaacttga ataatttcgc
     2101 accttaagag ggtaataata cagtagtttt attagcaaat atttatggtg tagaggc
//
LOCUS       DAVMGZ010000173         2138 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000173 DAVMGZ010000000
VERSION     DAVMGZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.174"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            87..1610
                     /locus_tag="VYQ93_001918"
     CDS             87..1610
                     /locus_tag="VYQ93_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336554.1"
                     /translation="MFSHILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDD
                     NAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGIILLALTIIGGLILGAMMGE
                     QEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFKSFYKAIKDYSKYAYDYSLS
                     CKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIMILFLSGVLFVSFMRMM
                     WYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNYNIEFENVSFAYNDKAVIEN
                     LSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSIKIGGIAISEYSDEALIKAI
                     SFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGCDLILDKFPERENTIIGSKG
                     VYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEFELQKAFKNLMKDKTVIMIA
                     HRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSLQEMFNSANEWRVSNERVL"
     gene            1576..>2138
                     /locus_tag="VYQ93_001919"
     CDS             1576..>2138
                     /locus_tag="VYQ93_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336555.1"
                     /translation="MNGGFQMKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAI
                     LLMIFAQEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTA
                     ENLSKLPLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAG
                     NVKMGLAVIIPSILSFIFIPLSKKYQV"
BASE COUNT          746 a          297 c          403 g          692 t
ORIGIN      
        1 attcaaactt atccggtgca gagagtatag cattaaaatc tgttattaca ctaacaagtg
       61 gagcgatatt ttattttgtc tcaggaatgt tttcacatat cttgggattt aggcttgaaa
      121 caaatttaag aaaaagggga atcgatggtc tggaaaaagc aagttttagg ttctttgact
      181 taaatccatc tggtcaaata agaaagatca tagatgataa tgcagcacaa actcatcagg
      241 tggtagcaca catgattccc gatagttctc aggcaataat cacacccgta cttgtacttg
      301 cacttggctt tatagtaagt ataagggttg gcataatttt gcttgctctt actataattg
      361 gtggcttaat tttaggggca atgatgggcg agcaagaatt tatgaagata taccaagaat
      421 ccctatctaa actaagtgct gaaactgttg agtacgtgag aggaatgcaa gttgtaaaaa
      481 tatttaaagc aaatgtagag tcttttaaaa gcttttataa ggcgataaaa gattactcaa
      541 agtatgctta tgattattcc ctatcttgta aaaggcctta tgttttgtat caatggttat
      601 tttttggact gattgcaatt ttaattattc ctatagttta ttttatgact agcttagcta
      661 gcgcaaaggt gattttactt gagcttatca tgattttatt tttatcagga gttctctttg
      721 tttcattcat gagaatgatg tggtactcca tgtatatttc tcaaggaaat tatgcagtag
      781 atactttaga ggcgctttac gaagatatgc aaaaagacaa attagtgcat ggtaatgtca
      841 ataattttaa aaactataat atagaatttg agaatgttag ctttgcttat aatgataaag
      901 ctgtcattga aaatttatcc tttaatttag aagaaggaaa gtcctacgca cttgtcggtt
      961 catctggatc aggcaaatca acagtagcaa aacttatatc aggtttttac aatgttaata
     1021 aaggaagcat aaagataggc gggatagcaa taagtgaata ttctgacgaa gccttaatta
     1081 aagccatttc ctttgttttt caagattcaa aattattcaa gaagagcatt tatgataatg
     1141 tagctttagc taataaagat gcgacgaaag atgacgttat gagagcctta aaattagcag
     1201 gatgcgattt aatattagac aaattcccag aaagagaaaa tacaatcata ggctcaaaag
     1261 gtgtttattt atccggtgga gaaaaacaaa gaattgcaat tgctagagca attttaaagg
     1321 attccaaaat tattattatg gatgaagcat cagcatctat tgacccagat aacgagtttg
     1381 aattgcaaaa agcttttaaa aatcttatga aggataaaac agttatcatg attgcacaca
     1441 ggctatctac aattaaagac cttgatgaaa ttattgtcat ggatagtgga aaaattatag
     1501 aaagagggtc tgacaaagaa ttaatgtcaa aagatacaag gtataagagc ctgcaagaga
     1561 tgtttaacag tgcgaatgaa tggagggttt caaatgaaag agttttataa aaaaagattt
     1621 gctcttacag atggaggagc aagaaattta agtaaagcaa cactggcttc atttttcgtt
     1681 tattgtataa acatgcttcc tgccatatta cttatgattt ttgctcagga agttttggaa
     1741 aatatgggca aaagcaatgg cttttatata gtattctcag ttttgacttt gatagcaatg
     1801 tatattttgc tttctatcga atacgataaa ttatataaca caacctatca agaaagtgca
     1861 gatttaagaa taaggacagc ggagaattta tcaaaattac ctctatctta cttttctaaa
     1921 catgacattt ccgacatttc acaaacaatc atggctgata ttgaaggcat agagcatgca
     1981 atgagccact caataccaaa ggtgggcggc atggtactgt ttttcccatt aatatctgta
     2041 atgatgctag cgggcaatgt caagatgggt ttagctgtaa ttattccatc tattttaagc
     2101 tttatattta tacctttatc taaaaaatat caggttaa
//
LOCUS       DAVMGZ010000174         2133 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000174 DAVMGZ010000000
VERSION     DAVMGZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.175"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            129..1622
                     /locus_tag="VYQ93_001920"
     CDS             129..1622
                     /locus_tag="VYQ93_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV3336556.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKVISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..>2133
                     /locus_tag="VYQ93_001921"
     CDS             1635..>2133
                     /locus_tag="VYQ93_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3336557.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDEL"
BASE COUNT          754 a          318 c          424 g          637 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg taatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcca
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggca
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg
     1741 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
     1801 cagatgatat tctgatagca tgggatggag ctaatgcagg aacagttggt tatggattat
     1861 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
     1921 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagaacatt
     1981 caacaggtgc aacaattcct catttaaaca agaatatatt acttgattta caattagaat
     2041 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaagggctta
     2101 ttactaaaag aaaattgcag ttagatgaac taa
//
LOCUS       DAVMGZ010000175         2104 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000175 DAVMGZ010000000
VERSION     DAVMGZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.176"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            39..110
                     /locus_tag="VYQ93_001922"
     tRNA            39..110
                     /locus_tag="VYQ93_001922"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71..73,aa:Gln,seq:ttg)
     gene            272..1474
                     /gene="rpsA"
                     /locus_tag="VYQ93_001923"
     CDS             272..1474
                     /gene="rpsA"
                     /locus_tag="VYQ93_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV3336558.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1559..>2104)
                     /locus_tag="VYQ93_001924"
     CDS             complement(1559..>2104)
                     /locus_tag="VYQ93_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3336559.1"
                     /translation="QHLSRAMSRVRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSD
                     KRFYRPTFRMHLTNKEILDKLLSYSEDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNL
                     KQAHPIFQTVFKTFLKDKEKIINALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFK
                     KRIFIALNIKKERTKFVLSQA"
BASE COUNT          634 a          346 c          482 g          642 t
ORIGIN      
        1 gttcgggggt tcgaatccct ctctctccat ttcattaatg gggtatagcc aagcggtaag
       61 gcaagggact ttgactccct catgcgttgg ttcgaatcca gctaccccag ttcttaggta
      121 ataatcaaga taaaaagcaa aatatcttag ggtattttat ttttataatt gaaagacgtg
      181 aatgatatga acatgtcctt gcgggtgctt aggaaaaaaa ttataagtat gtcaagttta
      241 agaaaaactt gattgttgga ggatttttta gatgaacgaa tttgaagatt tgctaaatag
      301 cgttagtcaa gttgagactg gtgatgttgt tagtgctgaa gtattgacag ttgatgcgac
      361 tcaagctaac gttgcaatct ctggaactgg tgttgaaggt gtcttgactc ttcgcgaatt
      421 gacaaacgat cgtgatgcag atatcaatga ctttgttaaa gtaggagaag tattggatgt
      481 tcttgtactt cgtcaagtag ttggtaaaga tactgataca gttacatacc ttgtatctaa
      541 aaaacgcctt gaagctcgca aagcatggga caaacttgtt ggtcgcgaag aagaagttgt
      601 tactgttaaa ggaacgcgtg ccgttaaagg tggactttca gtagaatttg aaggtgttcg
      661 tggatttatc ccagcttcaa tgttggatac tcgtttcgta cgtaacgctg agcgttttgt
      721 aggtcaagaa tttgatacta aaatcaaaga agttaacgct aaagaaaacc gcttcatcct
      781 ttcacgtcgt gaagttgttg aagcagctac tgcagcagct cgcgctgaag tattcggtaa
      841 attggctgtt ggtgatgttg taactggtaa agttgctcgt atcacaagct tcggcgcttt
      901 catcgacctt ggtggtgttg acggattggt tcacttgact gaattgtcac atgaacgtaa
      961 cgtatcacca aaatcagttg taactgttgg tgaagaaatt gaagtgaaaa tccttgatct
     1021 taacgaagaa gaaggacgtg tatcactttc acttaaagca acagtaccag gaccatggga
     1081 tggcgttgag caaaaattgg ctaaaggtga tgtagtagaa ggaacagtta aacgtttgac
     1141 tgacttcggt gcatttgttg aagtattgcc aggtatcgat ggacttgttc acgtatcaca
     1201 aatttcacac aaacggattg aaaatccaaa agaagctctt aaagttggtc aagaagttca
     1261 agttaaagtt cttgaagtta acgcagatgc agaacgcgtg tcactttcta ttaaagctct
     1321 tgaagaacgt ccagcccaag aagaaggaca aaaagaagaa aaacgtgctg ctcgtccacg
     1381 tcgtccaaga cgtcaagaaa agcgtgattt cgaacttcca gaaacacaaa caggattttc
     1441 aatggctgat ttgtttggtg atatcgaact ttaatcaaat tgaaaattca caaaatcctt
     1501 tgtttactaa acaagggatt tttctggctc tttgtcaact gtagtgggtt gaagtcagct
     1561 aagcttgaga aaggacaaat ttcgtccttt cttttttgat gttcaaagcg ataaaaatcc
     1621 gttttttgaa gttttcaaag tttcgaaaac caaaggcatt gcgcttgata agtttgatga
     1681 gattattggt cgcttccagt ttggcattag aatagtgtag ttgaagggcg ttgataatct
     1741 tttctttatc tttgaggaag gttttaaaga cagtctgaaa aataggatga gcctgcttaa
     1801 gattgtcctc aataagtccg aaaaatttct ctggttcctt attctggaag tgaaacagca
     1861 agagctgata gagctgatag tggtgtttca agtcttctga atagctcaaa agcttgtcta
     1921 aaatctcttt attggttaaa tgcatacgaa aagtaggacg ataaaatcgc ttatcactca
     1981 gtttacggct atcctgttgt atgagcttcc agtagcgctt gatagccttg tattcatggg
     2041 attttcgatg aaactgattc atgatttgaa cacgcacacg actcatagca cggctaagat
     2101 gttg
//
LOCUS       DAVMGZ010000176         2085 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000176 DAVMGZ010000000
VERSION     DAVMGZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.177"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(244..555)
                     /locus_tag="VYQ93_001925"
     CDS             complement(244..555)
                     /locus_tag="VYQ93_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336560.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(570..1856)
                     /locus_tag="VYQ93_001926"
     CDS             complement(570..1856)
                     /locus_tag="VYQ93_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV3336561.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDATQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQAGDMVRALKEGLINFYKEDGTSVTVRA"
BASE COUNT          631 a          447 c          367 g          640 t
ORIGIN      
        1 agacggattg ttttgacaat tctaatcttt tagactttgc ataataactg ttaaacagtc
       61 gaaaattacc ccagttttaa caagtagtca aaataccaga taaaggaaaa aagtctttaa
      121 aatcttaaaa acgcatagta tcaggtattg aacaatcttg atattatgcg ttttattgtg
      181 ggaagattta cttcattttc tcttgaaatt gagttttctc ctacttcaaa actagatgat
      241 ttgctattta aaatagcgtt ccttatgatt ttggcaacct ggattaaagc gagaaaaaca
      301 aaaggggcaa cttaaacttt ctttatattg cttatatgtt agtagtttta gacaaacacc
      361 acataaaata ggcttatcct gtatcaaaga taagggatac ggctcaaaag ggtgattttc
      421 taatgaatca tgacaccgat aacaagcata atacttttta caatcatagc attgtaaagc
      481 aataatatcc agcttgctat ggtaatgaat gcatcttgtt tgcttatcca ctaagaaacc
      541 ctgtgcctct atcatttcct tttcctttct taagctcgaa ctgtgacgct ggttccatct
      601 tccttataaa aattgatgag tccttctttt aatgcacgaa ccatgtctcc agcttgaacg
      661 ggttgtggga ctttaatagt caatagttcc attggatttg gagcgcggtc gattttattg
      721 cctttggcat catgcaaatc ttcaatatag gtttcaaaat gacggaaacc tggaccataa
      781 aactcaactt ggtccccttc gttaatgacg tttcgttgac gaatggttgc tgtttgtgtc
      841 gcatcatcat aagaaaccac ttcagcgaca aacttgtact cagggatttt acggcgagca
      901 ccaaacaact gctcattttc agatggtata ccgtagtaga aacctgttgc caattcacgt
      961 tgggcaacct tccacatctc gtccactaag tcttgtttga tagcttcaaa cttttcagga
     1021 ctttcaagat aggcatccac agccgccttg tagcagttgg ttactgttga tacgtagtga
     1081 atagacttca tacgtccttc gatttttaga ctgtccacac cattttcaat catatctgga
     1141 atgtggtcaa tcatagacat gtcaacggct gacattgaaa attcttctgg aatctcaccc
     1201 tgcaaactct tacgttcttt cccaaatggc atatcgtaaa ggtcgtattt ccaacggcat
     1261 gactgagaac atccaccacg gttggcatca cgcatactca tgtggtttga aagagtacaa
     1321 cgtccagagt atgaaataca catagctcca tggacaaagg cttcaatttc aacatctgta
     1381 cgtttgcgga tctcagctaa ttcttccatt gaaacctcac gcgctaaaac gacacgagtc
     1441 aagcctagct ctttccagaa ctcaagggtt tcatagttag tggcactggc ttgggtagaa
     1501 aggtggattt caaggcctgg tgcttcagtc gctgcaatca taatcaaggc tgggtcagat
     1561 acgataactg ctgcaatccc gatatcacgc agtttacgga accactcacc agcaccagct
     1621 tcatttcctt cgtgcataac catattagcc gctacataga ccttggcacc atacttggcc
     1681 gcaaactgca cgccttcttc catctgttcg aaagtaaagt ttcccgcacg gctacgaaga
     1741 ccataggcct gaccaccgat aaagacagca tctgctccat actgaacagc tacctttagc
     1801 ttctctaaag tccctgcagg tgataaaacc tcaggacgtt ttaatgtttt tgtcatgatt
     1861 tctcctattt gcaatataaa agtttgacat ttttccttct cattttatag taaataaagg
     1921 ataaaatcaa gcctagacgg attgttttga caattctaat cttttagact ttgcataata
     1981 actgttaaac agtcgaaaat tatcccagtt ttaacaagta gtcaaaaagt ctagaaaagt
     2041 aaaaaccagt atccaaagat actgactcgt aaaaatacat aaatt
//
LOCUS       DAVMGZ010000177         2044 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000177 DAVMGZ010000000
VERSION     DAVMGZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.178"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            17..337
                     /locus_tag="VYQ93_001927"
     CDS             17..337
                     /locus_tag="VYQ93_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV3336562.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            383..691
                     /locus_tag="VYQ93_001928"
     CDS             383..691
                     /locus_tag="VYQ93_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3336563.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            708..2006
                     /locus_tag="VYQ93_001929"
     CDS             708..2006
                     /locus_tag="VYQ93_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV3336564.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPTIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
BASE COUNT          615 a          286 c          433 g          710 t
ORIGIN      
        1 aaatgaggag gaatttatgg aaattattgt tccagatcaa attatcatgg gtttaatttt
       61 atatgctggc gatgcgaaac aacatattta taaagcatta gattacataa agaacggtac
      121 atgtgaacga tgtgaagaag aaatccagtt agctgatgca gccttattag aagctcataa
      181 tctacaaaca aaattcttgg cacaggaagc gtctggtaca aagacagaaa ttacagctct
      241 ctttgttcat tcacaagatc atctcatgac tagtatgacg gagattaatt taatcaaaga
      301 aattattagt ttgagaaaag aacttcataa aaaataatac tagagtatta tcattgttat
      361 taacatagag gaggaaaaca taatggtgaa gattggtttg ttttgtgcag caggtttttc
      421 tactggtatg cttgtaaata atatgaaaat tgcagcgcaa tctagtggag ttgaggcaga
      481 aatagaggcg ttttctcagt ctaaattagc ggattatgcg ccaaatatag atgttgcact
      541 attgggtcca caagttgctt atacattaga taaatcaaaa gaaatttgtg ataagtgtga
      601 tgttccgata gctgttattc cgatgatgga ctatggtatg ttagatggga aaaaagtatt
      661 agatttggcc ctatctttga ttagtgggta agaaaaggag atttattatg tcaaagatgg
      721 atgttcagaa aatcattgca ccgatgatga agtttgtgaa tatgcgtggc attatagctc
      781 taaaagatgg gatgttagca attttaccat tgacagtagt tggtagtttg ttcttgatta
      841 tgggacaatt gccgttcgaa ggattaaata agagcattgc tagtgttttt ggagctaatt
      901 ggacagagcc gtttatgcaa gtatattcag gaacttttgc tattatgggt ctaatttctt
      961 gtttttcaat tgcctattct tatgctaaga atagcggagt agaggcttta ccagctggag
     1021 ttctatctgt atctgcattc tttattttgc taagatcgtc ttatatccct aaacaaggtg
     1081 aggcgattgg ggacgctatt agtaaagttt ggtttggagg ccaaggaatt atcggtgcta
     1141 tcattatagg tttggtagta ggaagtattt ataccttctt tataaagaga aaaattgtta
     1201 ttaagatgcc agaacaagtt ccacaagcta ttgccaaaca gtttgaagca atgattccag
     1261 catttgtaat tttcttatct tctatgattg tatatatttt agcgaagtca ttgactaatg
     1321 gcggaacatt catagaaatg atttattctg ctattcaagt tccgttgcaa ggtttaactg
     1381 gatctttgta tggtgctatt ggaattgcat tctttatatc atttttgtgg tggtttggtg
     1441 ttcatgggca atcggtagta aatggagtag tgacagctct gcttttatct aatcttgatg
     1501 ctaataaagc tatgttagcc tctggtaatc tatcattaga aaatggtgca catattgtta
     1561 ctcaacaatt tttagattca tttttaattc tatcaggttc agggattacg tttggtcttg
     1621 tagttgccat gctttttgca gcaaaatcaa aacaatacca agccttagga aaagttgcag
     1681 cttttccaac aatatttaac gtaaatgagc cagttgtatt tggatttccg attgtcatga
     1741 atccagttat gtttgtacct ttcattcttg ttcctgtact tgcagctgtg atagtatatg
     1801 gagctattgc aacaggtttc atgcaaccat tctcaggggt aacattgcct tggagtacac
     1861 cagctattct atcaggattt ttggtgggtg gatggcaagg tgttgtgact cagttattag
     1921 tattggtgat atctacagtg gtttactttc cattctttaa gatacaagat cgtttagctt
     1981 accaaaatga aatcaaacaa tcttagaggt atttgtgtgt tactgttaaa ctcacacatt
     2041 tgtg
//
LOCUS       DAVMGZ010000178         1866 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000178 DAVMGZ010000000
VERSION     DAVMGZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.179"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            33..965
                     /locus_tag="VYQ93_001930"
     CDS             33..965
                     /locus_tag="VYQ93_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3336565.1"
                     /translation="MSGAVVKNLNSDKVASILIPLPPLSEQQRIIRAIESALEKVDEY
                     AESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEE
                     GKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPI
                     YPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYH
                     ENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLT
                     KTIKEYLIPLPPLPEQSRIVDKIEQFFAHINALI"
     gene            1022..1819
                     /gene="psrA"
                     /locus_tag="VYQ93_001931"
     CDS             1022..1819
                     /gene="psrA"
                     /locus_tag="VYQ93_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV3336566.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT          669 a          272 c          330 g          595 t
ORIGIN      
        1 caaatgtagt ttattctcaa tttctatctc taattagtgg agctgttgtg aaaaacttga
       61 atagtgataa agttgcttct attcttatcc ctctcccccc actatccgaa caacaacgca
      121 taataagagc aatcgaatca gctttagaaa aagtagatga atatgctgaa agttataata
      181 gactagaaca gctagataaa gaatttccag ataaactaaa aaaatctatt cttcaatatg
      241 ctatgcaagg aaaattagtt gaacaagacc caaatgatga atcagtcgaa gttttacttg
      301 aaaaaatacg agcagaaaaa caaaaactct ttgaagaagg caagattaaa aagaaagatt
      361 tggacatttc tattgtttcc caaggagatg ataactctta ttatgaggaa gtaccttgtg
      421 aaataccaga aagttgggaa tgggtgaggt taaacgatat aacctcctac attcaaagag
      481 ggaagtctcc aaaatattca aatattccta tataccctgt gattgctcag aaatgtaatc
      541 aatggtctgg cttttctatt gacttagcaa gatttattga tcccgaaaca gttcatagtt
      601 accagaaaga aagattacta agagatggtg atttaatgtg gaattcaaca ggtctaggta
      661 cgttaggacg attagctatt taccatgaaa ataaaaatcc ttatggctgg gctgttgcag
      721 atagccatgt tacagttata agagtcttat cgggggttat taattgccat ttcatctata
      781 attttctttc ctctcctatt gttcagtcag ttattgaaga aaaagcgtcg ggtagtacaa
      841 aacaaaaaga attattaacg aaaactataa aagaatattt aatccccctc ccaccccttc
      901 ctgaacaatc cagaatcgtt gataaaatcg aacaattttt cgcccatatt aacgcactaa
      961 tttagtataa tagttttacg tgcggtggaa tttctatcaa acataagata ggagttttac
     1021 gttgaaagat tttgaattac atttgaagaa agctggttta gctgaaaata ctgtgcgttc
     1081 gtacctttat ggtgttcgat ttttcttgga gaattacgaa ctcaaaatgg aagatttatt
     1141 tgaatacaaa ggatatttat tagacaattt taaacctaaa actgtcaatt tgcgactaca
     1201 aggagtaaat aaatatctgg cttttattgg tcatgatgat ttgaaattaa aatttgtcaa
     1261 agtacagcaa aaaccgtttt tagaagatgt cattagccat gctgattacc tttttcttaa
     1321 acgtagtttg aaaaaagatg gtattttaaa atggcatttt gtcgtttggt ttttaggtgc
     1381 aactggggct cgtgtaagtg aacttattaa actaaaagta gagcatgtag aaatcggtta
     1441 ttttgacatt tactcaaaag gtggtaaaat tcgtagactg tacattccta aaaaattaag
     1501 aaatagttgt cttagttggt tagaatcaga aaatcgccga agtggttatt tatttttgaa
     1561 taaatttaac gaaccaatta cagcaagagg agttgctcaa cagttaaaaa attatgctga
     1621 taaatacaaa atgaatccta aagtaattta ccctcattct tttaggcatt tatttgctaa
     1681 gaatttttta gcgaagtata atgatattgc cttgcttgca gatttgatgg gacacgaaag
     1741 tatagaaact actcgaattt atctaaggaa aacagctact gaacaacaaa atattgtaga
     1801 taaaattgtt aattggtaaa aaataacagg tggtcaaact gactacctgc tatttttgtg
     1861 attatg
//
LOCUS       DAVMGZ010000179         1860 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000179 DAVMGZ010000000
VERSION     DAVMGZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.180"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            103..1734
                     /locus_tag="VYQ93_001932"
     CDS             103..1734
                     /locus_tag="VYQ93_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV3336567.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGIGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNIIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          538 a          393 c          362 g          567 t
ORIGIN      
        1 aattttcttt tacaaacttg acattaacac ttgttagatt tacttttatc ctttaaaata
       61 gaataggaga tattccgcga ttatttttcg gaggaaaaag aaatgtccat taattggcag
      121 gaaattttat ttcacttttt aggtggtctg gggctattct tatatagcat caagaccatg
      181 ggagacggtt tgcaacaagc tgctggagat cgccttcgtt tttacattga caaatatact
      241 agtaatcctt tctttggagt tctggttggt attgggatga ctgctctaat tcagtctagt
      301 tctggtgtaa cagttatcac agtcggcctg gtcagtgccg gtctcttaac cttacgtcag
      361 gctatcggga ttgtcatggg tgctaatatt gggacaactg tcacatcctt tctcatcggt
      421 tttaaattag gtaactatgc cctacctatg ctctttatcg gtgccgtctg tctctttttt
      481 acaaaaaatc ggacagtcaa taatatcgga cgcatcctct ttggtatcgg tggtatcttt
      541 tttgccctca atctcatgag cggcgcaatg gctccactca aggatttaca ggtctttaag
      601 gactatatga ttgagctaag taagaatcct gttttgggtg tctttgtcgg tactggcttg
      661 accttgctaa ttcaagcttc ttcggctacc attgggattt tacaaaacct ctacgccggc
      721 aatctaattg acctacaggg agctttgcca gttctatttg gtgacaatat cgggacaacc
      781 attacagcca tcattgcctc tttaggggct aatattgcag ctaaacgggt agcaggagct
      841 catgttgcct tcaacattat cggaacagtc gtctgcgtta tttttctagt tccttttact
      901 gtcctgattc attggtttga agctacgcta aatctagcac cggaaatgac catcgccttt
      961 gctcacggaa cctttaatat taccaacacc attgtccaat ttccatttat cggagctctg
     1021 gcttactttg taaccaagat tattcctgga gaggacgagg ttgtcaaata cgaaccctta
     1081 tatcttgatg aacatttcat caaacaggcc ccatctatcg ctctaggaaa tgctaagaaa
     1141 gagctcttgc acttaggaaa ctacgctgct aaagcctttg acctttccta taagtacatc
     1201 attgacttgg atgaaaaagt tgctgaaaaa gggcataaaa ccgaagaagc aattaacacc
     1261 atcgatgagc aattaacacg ttatctcatt gccctttcaa gcgaagctct cagccaaaaa
     1321 gaaagtgaag tgcttaccaa tatccttgat tcctcccgtg atttggaacg gattggagac
     1381 cacacggagg ctctactcaa tctgactgac tatcttcaac ggaaaaatgt tgaattttct
     1441 gatgccgcct tgaaagaatt agaggaagtt taccgccaaa ctagtgactt tatcaaagat
     1501 gctctggata gtgtggaaaa caatgatatt gaaaaagcac gcagtcttgt agaacgtcat
     1561 gaagcaatca ataagataga acgtgttctc agaaaaaccc acatcaaacg cctcaacaaa
     1621 ggcgaatgtt caacacaagc tggagtcaac tttatcgaca tcatctcaca ctacactcgt
     1681 gtatcagacc acgctatgaa ccttgctgaa aaggtttttg cagaacaaat ctaataacca
     1741 agaagctatc catcataatt ggatggcttc ttactttttc ctaagcaaga ctaggctgaa
     1801 tgaaactgaa agagtattct gcagatatat aatatattga aactagaata gtacacctct
//
LOCUS       DAVMGZ010000180         1783 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000180 DAVMGZ010000000
VERSION     DAVMGZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.181"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            389..1261
                     /locus_tag="VYQ93_001933"
     CDS             389..1261
                     /locus_tag="VYQ93_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV3336568.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(1407..1751)
                     /locus_tag="VYQ93_001934"
                     /pseudo
     CDS             complement(1407..1751)
                     /locus_tag="VYQ93_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          577 a          318 c          341 g          547 t
ORIGIN      
        1 ttgagtttgg aatagtacgc tgtagctgct aaaacatttc taggaattaa tttgcctttc
       61 ctaatagagt tgttcatatc ttatttcaat ctactatatt tttcacctct aacaatcgca
      121 atctcttctt tatccatgaa cgaaaacgct ttctatcttt gtaagtaaag catcacacga
      181 aatccacgaa aatgaaaacc tttgttgtgt tttcgtaaaa aatttgttga cagagcacga
      241 aacgcctact ataattaaat caagtttaag gaaataacaa aaaaacaaat cggagggaaa
      301 gacgatgaac acattaaatg agaaagtaat caatatctgt aaagcagtag ttaaagaaac
      361 tttaatccaa gacatttagg agaaagaaat gaagaaatca agaaaattag ccacattagg
      421 aatctgttca gccttgtttt taggcttggc tgcttgtcaa caacaacatg ctacttctga
      481 ggggacgaat caaaggcaaa gcagttcagc gaaagttcca tggaaagctt catacaccaa
      541 cctaaacaac caggtaagta cagaagaggt caaatctctc ttatcagctc acttggatcc
      601 aaatagtgtt gatgcatttt ttaatctcgt taatgactat aataccattg tcggctcaac
      661 tggcttatca ggagatttca cttcctttac tcacaccgaa tacgatgttg agaaaatcag
      721 tcatctctgg aatcaaaaga agggcgattt cgttgggacc aactgccgta tcaatagtta
      781 ttgtcttttg aaaaattcag tcaccattcc aaagcttgaa aagaatgacc agttgctttt
      841 cctagataat gatgcgattg ataaaggaaa ggtctttgat tcacaagata aggaagagtt
      901 tgatattcta ttttcgagag ttccaactga ggcaactaca gatgtcaagg ttcacgctga
      961 aaagatggaa acattcttct cacaatttca attcaatgaa aaagctcgaa tgctgtctgt
     1021 agtcttgcac gacaatctgg atggcgagta tctgtttgta ggccacgttg gggtcttagt
     1081 acctgctgat gacggtttct tatttgtaga gaaattgact ttcgaagagc cctaccaagc
     1141 gattaaattt gctagtaagg aagattgcta caagtatttg ggcaccaagt atgcggatta
     1201 tacaggcgag ggactggcta agccttttat catggataat gataagtggg ttaaacttta
     1261 atagtgttat gatatgacag taatcttagg atttttattt ctatttttga tgacttggtg
     1321 ggttatttca atcgttaata gccagaaacc acgcaaaaat gaaacattta ttggctatat
     1381 ccaaagatac gatattgatg ggaatggcta tcaatcatta ccgtgtcctc agaattgaga
     1441 ggagttcttg aaatcgtaaa actactttga acaagagtta cttcaaccca ttggctccga
     1501 cggattaagt tgctttcgtg aacaccaaaa tcagccgcaa tttcttcata agtgcggtat
     1561 tctcgcacat attgaagagt ggccataaga aggtcttcta ggcttaattt aggttttcgt
     1621 cccccttttg tgtgtttaag ttgataagca gtttttaata cagctaacat ctcttcaaaa
     1681 gtggtgcgct gaacaccaac aagacattta aatcgtgcat cagttagttg cttacttgct
     1741 tcataattca tagaactact ataccatatt ttgtttcgca gga
//
LOCUS       DAVMGZ010000181         1752 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000181 DAVMGZ010000000
VERSION     DAVMGZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.182"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..266
                     /locus_tag="VYQ93_001935"
     CDS             <1..266
                     /locus_tag="VYQ93_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3336569.1"
                     /translation="ASQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYWT
                     FLSGKAEKQGITLYHHDQCRSGSVVQEFLGDYSGYVHCDILRQ"
     gene            361..790
                     /locus_tag="VYQ93_001936"
                     /pseudo
     CDS             361..790
                     /locus_tag="VYQ93_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            861..1019
                     /locus_tag="VYQ93_001937"
     CDS             861..1019
                     /locus_tag="VYQ93_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEV3336570.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            1112..1752
                     /locus_tag="VYQ93_001938"
                     /pseudo
     CDS             1112..1752
                     /locus_tag="VYQ93_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          557 a          320 c          387 g          488 t
ORIGIN      
        1 aggcgagtca atactatttg gagccccttt ataacctctt gcgagagaga ctattgactc
       61 agcccttact tcatgcggat gaaacttctt atagggtgct agagagtgat agtcagctga
      121 cttactattg gacttttttg tcaggtaaag cagagaaaca agggattacg ctttaccacc
      181 atgatcagtg tcgaagtggt tcagtagtac aagaattcct aggagattat tctggctatg
      241 tgcattgtga tattttgcgg cagtaactta ggactttagt cctctagttc tgcctatgcg
      301 atagcagtcc aaggtttagg agcaaggcga cgctaagctt ggtaaacttc gaaccgctcg
      361 tctgcttatc gtcaactgga agaagctgaa cttgttggat gttgggcgca tgtgagaagg
      421 aagttttttg aagcgccccc ccaagcaagc ggataaatca tccttaggag ctaaaggttt
      481 agcttattgt gatcagttat tttccttgga aagagactgg gaggctttgc cagctgatga
      541 acgactacag aaacgtcaag aacatctcca gcccttaatg gaagacttct ttgcttagtg
      601 ccggcgtcag tcagttttag caggttcaaa actaggaagg gcaattgaat acagcctcaa
      661 gtatgaagaa acctttaaga ccattttgaa agacggacat ctggtccttt ccaataatct
      721 agctgaacgc gccattaaat cattggttat gggacggagt aaaagagtcc agtggactct
      781 tttagcctaa gctcagttta aaaaagcgag ggtggttatt ttctcaaagt tttgaaggag
      841 ctaaagcaag agctattatt atgagtttgt tggaaacagc taaacgtcat caattaaata
      901 gcgagaaata tctatcctat cttctagaat gtcttccaaa cgaggaaact ctcgtaaaca
      961 aagaggtttt agaggcttat ttaccatgga ctaaagttgt acaagaaaag tgcaaataag
     1021 aaatctccag attaggaact atccgtgagt tctccagtct ggagattttt caatagactt
     1081 cctgcgaaac aaaatatggt ataatagttc tatgaatgat gaagcaagta aacaactaac
     1141 cgatgcacga tttaagcgtc ttgttggtgt tcaacgcacg acttttgaag agatgttagc
     1201 tgtattaaaa acagcttatc aacttaaaca cgcaaaaggt ggacgaaaac ctaaattaag
     1261 tctagaagac cttcttatgg ccactcttca atatgtgcga gaatatcgaa cttatgaaca
     1321 aattgcggct gtttttggta ttcacgaaag caacttaatc cgtcggagcc aatgggttga
     1381 agtaactctt gttcaaagtg gtgttacgat ttcaagaact cctctcagtt ctgaggacac
     1441 ggtaatgatt gatgcgacgg aagtaaaaat caatcgccct aaaaaaagaa ttagcgaatt
     1501 attctggtaa aaagaaattt cacgctatga aggctcaagc gattgtcaca agtcaaggga
     1561 gaattgtttc tttggatatc actgtgaact attgtcatga tatgaagttg ttcaaaatga
     1621 gtcgcagaaa tatcagacaa gctggtaaaa tcttggctga cagtggttat caagggctca
     1681 tgaagatata tcctcaagca caaacttcac gtaaatccag caaactcaaa ccgctaacaa
     1741 ttgaagataa ag
//
LOCUS       DAVMGZ010000182         1718 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000182 DAVMGZ010000000
VERSION     DAVMGZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.183"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..944)
                     /locus_tag="VYQ93_001939"
     CDS             complement(<1..944)
                     /locus_tag="VYQ93_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV3336571.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HTEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFL"
     misc_binding    1276..1505
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          500 a          333 c          318 g          567 t
ORIGIN      
        1 aaaagaaatt cacttggcga cttgtagtcc aagcattttt tagggtagtt gttaatccaa
       61 ttttcgataa aagctacttc tttcggagtc gttttcttgg ttcctttagg taaccatctc
      121 cgaattaatc gattatgatt ttcatttgaa cctctctccc atgaagagta agcatgtgcg
      181 tagtagatat gttcctcagg aaatacctca gacaatcgtt tgaactccga accattatct
      241 gcagtaatgg acagaatacg atgctcccct aatagtaacg taagcgcctg attgacagat
      301 tcagcagttt tatttggaat taaccttata atttggtgtc tgctccgccg gtcggttaag
      361 actaacaggc aataattttt tatcttagtc agtaggacgg tatcaatttc ataatgacca
      421 ttttccaagc gaagattaat aacgtcagga cgttcttcga tagatttacc tgccggctta
      481 aagttcggac tagcttgctt cttgacacct ttcctttttc tgggataaag catgtcggcc
      541 ttcgtcaatc ctaaatgacc attatgaaac cagtagtaga tggttgaaat accaactttc
      601 acttgcttct tgttaaccat catttcaggc gaaaattttt gcttatgata gtgtaagatc
      661 ttctctctga tttcctttgt taaaattaac tttttcaccg accgttttcg attgaattgg
      721 taaacagttt gtgcgtaatc ggcagaatag acctttttgt atagcccttt tctcacttgt
      781 tgtaaagttg tacctctttt gacttcagta tgaatcgttt gaggcgcctt tcctaacaag
      841 cctgcaattt cacgattgga cttattttca ttcttccacc gctcaatcaa gcgacggtta
      901 tctattgtca aatgtttccc ttttggggta taatgttctt gcatctctgt gcctttcctt
      961 gtgtttgtgg tgaacaacaa gtatagcaca gaggtgtttt cttatacctt aaatcacatt
     1021 tcatttgaca acaggaaccc ttgaacctgt tttttcagct aaaccaaggc tatgattagc
     1081 cttggccacc tagcctaaca gtttcaaacc tgttatcaaa tgaaatcatc aacttactca
     1141 aacatagtga aacatctaat tcttgactaa ggtaaggtgg ctaacttcat tatagaactt
     1201 tcaatggtca cattcgaatg atattttcaa aaaaatcgta aaaaagtctt gacaaagaaa
     1261 aaacaaagta ttaaaataag ttcaacaaat cagaaaagta actatttttg atcttcaggg
     1321 agcctgtggt ggttgtgaat aggtggttgg aagtagtgaa agtgggctga ttttgaaaat
     1381 gaatttgaaa caatgaaaat tcggtgcgca caccttacag tgcagcttgt tgttaaacaa
     1441 ggcagagatg taaagggata gtccctttat aattgaggtg gcaccgcgtt accaacgccc
     1501 tcacacggaa gtatattctg tgtgtgggct tttttctatc cgtcgtttgg tttatctttt
     1561 attagggcct taagtcgctt tgatgaactt gagttctatc tacagctcct ttcctcctac
     1621 taaaagcaaa caaaaagctg gactaatacg aaaaaagcta gtgatactca atgaaaatca
     1681 aagaacaaac taggaaacta tccgcaggtt gctcaaaa
//
LOCUS       DAVMGZ010000183         1703 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000183 DAVMGZ010000000
VERSION     DAVMGZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.184"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..600)
                     /locus_tag="VYQ93_001940"
     CDS             complement(<1..600)
                     /locus_tag="VYQ93_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3336572.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKD"
     gene            complement(656..1678)
                     /locus_tag="VYQ93_001941"
     CDS             complement(656..1678)
                     /locus_tag="VYQ93_001941"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV3336573.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
BASE COUNT          497 a          381 c          300 g          525 t
ORIGIN      
        1 atctttttgc aatttcattc ctttaaagat atatggttcc cccttaatac caacttgtga
       61 gtatggtttt gagaatggta ctacgttact tacaactgga gaaccaccag atgaagctgt
      121 tggcatcaat aatgaactat ctgtcgacca agcttgagct ttggcatatt tttcatatct
      181 cttctctagg tcagtggtct cagaaacagc atcttctaac aatttcttat atttatccaa
      241 accaggttta gctacaacat ccttatcttt tcctttcgta ataccaaggt gtttcatggc
      301 agaaccagat tttggatcca taatattcaa gtaagacgct ggatcttgat agcttggagc
      361 ccatcctgta ctattcaaat catagtcttt ttgagaagga gcaacattgc cgtatttatc
      421 attttccatc aaaccatcaa taacatttcc aataacgtct gtcctcgatg ttcgagtcgc
      481 tatactgtag cccaatgatg ctggatctac tgcatagaca taagaaaatg ttgtcggtgc
      541 atctgcttct ttatcagttt ttccacaagc cactaaaata gctgacgtgc tcaggaccac
      601 tcctgctgtt aagagccact ttttctattt cataaaaaat ctcctttggt ttattttaat
      661 ctacttttac aatccaacct tctggcgctt caatatcgcc aaactgaata cccgtcaatt
      721 cattatataa tttacgcgtc acaggaccta cttctgtttc actatagaat acatggaaat
      781 catcaccatg ttgaatacct ccaattggag aaataaccgc tgctgtacca caggcacctg
      841 cctctacaaa acggtcaaga ttatcaattg gaacatcacc ctcaatagga gttaatccca
      901 agcgatgttc tgccaaataa agcaaggaat acttggtaat agatggcaag atagatggac
      961 tcaatggtgt tacaaattca ttatcagctg taattccaaa gaagttagct gatccgactt
     1021 cttcaatctt tgtatgagtt gatgggtcca gatagataac atctgagaaa tgacgtgact
     1081 tggccatttt tcctggtaag agacttgcag catagtttcc accaacctta gccgcacctg
     1141 taccatttgg tgctgcacgg tcgtactcat cctgaatcaa gaagttggtt gggaccaaac
     1201 cacctttaaa gtaatttcca actggcatag caaagatggt gaaaatgtac tcttctgccg
     1261 gttttacccc gataatatct ccgacaccaa tcaaaagagg gcgaagatat aaggttccac
     1321 ctgttccgta tggtggtacg tattcttcat tcgcacggac aactgcttta caagcttcta
     1381 caaacatgtc tgtcggaact tgtggcatca agagacggtc acatgtacgt tgcagacgtt
     1441 tagcattttc atcaggacgg aacagttgaa cactgccatc cttagtacga taagctttca
     1501 aaccttcaaa tgcttgttgt ccatagtgaa gacttggaga agactctgaa atatgcaaag
     1561 ttgcatcctc tgtaagctct ccttgatccc attgtccatt tttgaaatga gcaagatagc
     1621 gataaggtaa tttcatatag gaaaaaccga ggttttccca atcaatcgtt actgtcatat
     1681 tccactcctt attctaaaaa cct
//
LOCUS       DAVMGZ010000184         1678 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000184 DAVMGZ010000000
VERSION     DAVMGZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.185"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            59..361
                     /locus_tag="VYQ93_001942"
     CDS             59..361
                     /locus_tag="VYQ93_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3336574.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            369..1170
                     /locus_tag="VYQ93_001943"
                     /pseudo
     CDS             369..1170
                     /locus_tag="VYQ93_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1219..>1678
                     /locus_tag="VYQ93_001944"
     CDS             1219..>1678
                     /locus_tag="VYQ93_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV3336575.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEV"
BASE COUNT          553 a          299 c          351 g          475 t
ORIGIN      
        1 atagatgaag caatcgctta atcttgtcta ttaaaaaata aaatcggagg aagtctatat
       61 gattaaaatt cttgctgcct gcggtgcagg tgttaactca agtcaccaaa ttaaaagtgc
      121 tctagaagaa gaactttcaa accgtggtta cgatgttcac tgtgatgcag ttatggtgaa
      181 agatgtaaac gaagacctta tgaaaggtta cgatatcttt acaccaatcg ctgcaacaga
      241 ccttggtttt gaaccaggta tcccagttat cgaagctggg ccaatcttat tccgtatccc
      301 agcaatgagc gttccagtat ttgacaatat tagacttcct gcgaaacaaa atatggtata
      361 gtagttctat gaatgatgaa gcaagtaaac aactaactga tgcacgattt aagcgtcttg
      421 ttggtgttca gcgtaccact tttgaagaga tgttagctgt attaaaaaca gcttatcaac
      481 ttaaacacgc aaaaggtgga cgaaaaccta aattaagcct agaagacctt cttatggcca
      541 ctcttcaata gtgcgagaat atcgaactta tgaacaaatt gcggctgatt ttggtattca
      601 cgaaagcaac tttatccgtc ggagccaatg ggttgaagta actcttgttc aaagtggtgt
      661 tacggtttca agaactcctc tcagctctga ggacacggta atgattgatg cgacggaagt
      721 acaaatcaat cgccctaaaa aagaattagc gaatgattct ggtaaaaaga aatgccacgc
      781 tatgaaggct caagcgattg tcacaagtca agggagattg tttctttgga tatcactgtg
      841 aactattgtc atgatatgaa gttgttcaaa atgagtcgca gaaatatcgg acaagctggt
      901 aaaatcttgg ctgacagtgg ttatcaaggg ctcatgaaga tatatcctca agcacaaact
      961 ccacgtaaat ccagcaaact caagccgcta acagttgaag ataaaaccta taaccatgcg
     1021 ctatctaagg agagaagcaa ggttgagaac atctttgcca aagtaaaaac gtttaaaatg
     1081 atttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa tttgattgct
     1141 ggcattatca atcatgaact aggattctag ttttgcagga agtctaataa agaaactagc
     1201 aaggtcaggc cttactttat tggtggacat ccaggattta attgtcctct acttgatgat
     1261 gaagtctatg aagattatta tctagagttt gagaaagaag agacttgctc tgttccacgt
     1321 cctttcccag aaacaggtat gttggatttc caagatagaa gtccatggct agagggacaa
     1381 aaagaaatag atcttagtta tgatcttttc agcaaagatg cagtgacttt ggatgaattg
     1441 caatctcgaa caattgccct tcgttctctt aaacatgata ggggattgaa agtgcatttt
     1501 gcagaatttc caaacctcat catctggtca actttgaaca agggaccttt cattaccttt
     1561 gaaccatggt ctggcttgtc aacattcctt gaagaaggag atcatttaga agataagaag
     1621 aatgtttgtc tcttagaagc caatcaggtt gaagaattag ggtttgagat agaagttt
//
LOCUS       DAVMGZ010000185         1636 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000185 DAVMGZ010000000
VERSION     DAVMGZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.186"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            84..491
                     /locus_tag="VYQ93_001945"
     CDS             84..491
                     /locus_tag="VYQ93_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3336576.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATPFKEVQKPLENFN
                     HIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            656..955
                     /locus_tag="VYQ93_001946"
     CDS             656..955
                     /locus_tag="VYQ93_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV3336577.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1216..1539
                     /gene="tnpB"
                     /locus_tag="VYQ93_001947"
     CDS             1216..1539
                     /gene="tnpB"
                     /locus_tag="VYQ93_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV3336578.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          556 a          325 c          285 g          470 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact cctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tcaaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattgacta atactcttcg aaaataaaat tcaaaccaca tcagcgtcgc cttaccatac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttt attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcagctag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag gcctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataata aaacgcatag
     1021 tagcaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcggag
     1201 gaaaagtatt gtactatgat gattcaacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgataca aggaatcgat tcactggctt atctcgttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     1381 aaagcccttt actgggatgg tcaaggattt tggatactat ataaacgctt tgagaacgga
     1441 aaactgactt ggcccagtac agaaaaggat gtcaaagctc tcgcacctga acaagtagac
     1501 tggcttatga agggcttttc tatcactccc caaatatagt ggatttaaac tagaatagta
     1561 cacccctact tctaaaacat tgttagaaat cgatttgact gtcctgatca atttgtcctg
     1621 ttcttgtttc atttta
//
LOCUS       DAVMGZ010000186         1597 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000186 DAVMGZ010000000
VERSION     DAVMGZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.187"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>1597
                     /locus_tag="VYQ93_001948"
     CDS             <1..>1597
                     /locus_tag="VYQ93_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV3336579.1"
                     /translation="VDDFTSETGKRIEEYDYKYDDKGNIIAYDDGSALQYETEKLDEI
                     KSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEIKATKYDSHSKTMTFDL
                     YANINDIVDGLAFAGDMRFFVKDDNRIKAETKIRMPEKNKETKAEYPYVSSYGNVIEL
                     GEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKVTTYNPGKTDMLE
                     GNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLMSALDGFNIIRYQ
                     VFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEAIKEDGSMLFIDT
                     KPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVNESVVDNYLIYGD
                     LHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYLQTGYSDLNAKAV
                     GVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFDGEIQEQHIYVNG
                     KEYTSFDDIKQITDKTLNIKIVVK"
BASE COUNT          639 a          195 c          307 g          456 t
ORIGIN      
        1 gttgatgatt ttacaagtga aactggtaag agaatagagg aatacgatta taaatacgat
       61 gataagggaa atattattgc ttatgacgat ggtagtgcct tacaatatga aactgaaaaa
      121 cttgacgaaa taaaatcaaa aatttatggc gttctaagcc catctaaaga tggacacttt
      181 gaaattcttg gaaagataag caatgtttct aaaaatgcca aggtatatta tggaaatagc
      241 tataaatcga tagaaatcaa agcgactaag tatgactcac attcaaaaac gatgacattt
      301 gatttatacg ctaatattaa tgatattgtg gatggattag cttttgctgg agatatgcgg
      361 ttttttgtta aagatgataa tcgaataaaa gctgaaacta aaattagaat gcctgaaaaa
      421 aataaggaaa ctaaagcaga atatccctat gtatcaagtt atgggaatgt aatcgaatta
      481 ggagaaggag atctttcaaa gaacaaacca gacaatttaa ctaaaatgga atctggtaaa
      541 atctattctg attcagaaaa acaacaatat ctgttaaagg ataacatcat tctaagaaaa
      601 ggctatgcac taaaagtgac tacctataat cctggaaaaa cggatatgtt agaaggaaat
      661 ggagtctata gcaaggaaga tatagcaaaa atacaaaagg ccaatcctaa tctaagagtc
      721 ctttcagaaa caacaattta tgctgatagt agaaatgttg aagatggaag aagtacccaa
      781 gctgtattaa tgtcggcttt ggacggcttt aacattataa ggtatcaagt gtttacattt
      841 aaaatgaacg ataaagggga agctatcgat aaagacggaa atcttgtgac agattcttct
      901 aaacttgtat tatttggtaa ggatgataaa gaatacactg gagaggataa gtccaatgta
      961 gaagctataa aagaagatgg ctccatgtta tttattgata ccaaaccagt aaacctttca
     1021 atggataaga actactttaa tccatctaaa tctaataaaa tttatgtacg aaatccagaa
     1081 ttttatttaa gaggtaagat ttctgataag ggtggtttta actgggaatt gagagttaat
     1141 gaatcggttg tagataatta tttaatctac ggagatttac acattgataa cactagagat
     1201 tttaatatta agctgaatgt taaagacggt gacatcatgg actggggaat gaaagactat
     1261 aaagcaaacg gatttccaga taaggtaaca gatatggatg gaaatgttta tcttcaaact
     1321 ggctatagcg atttgaatgc taaagcagtt ggagtccact atcagttttt atatgataat
     1381 gttaaacccg aagtaaacat tgatcctaag ggaaatacta gtatcgaata tgctgatgga
     1441 aaatctgtag tctttaacat caatgataaa agaaataatg gattcgatgg tgagattcaa
     1501 gaacaacata tttatgtaaa tggaaaagaa tatacatcat ttgatgatat taaacaaata
     1561 acagacaaga cactaaacat taagattgtt gtaaaag
//
LOCUS       DAVMGZ010000187         1588 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000187 DAVMGZ010000000
VERSION     DAVMGZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.188"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(36..577)
                     /locus_tag="VYQ93_001949"
                     /pseudo
     CDS             complement(36..577)
                     /locus_tag="VYQ93_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(653..1429)
                     /locus_tag="VYQ93_001950"
                     /pseudo
     CDS             complement(653..1429)
                     /locus_tag="VYQ93_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1353..1547)
                     /locus_tag="VYQ93_001951"
     CDS             complement(1353..1547)
                     /locus_tag="VYQ93_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3336580.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLSLFEEEQNMEEDSDLPS"
BASE COUNT          425 a          354 c          303 g          506 t
ORIGIN      
        1 cagtcgatgc gtactgttat agattcaacg cactataatt gatgacgttt agctgtttcc
       61 aacaaactca taataatagc tcttgcttta gctccttcaa aactttgaga aaataaccac
      121 cctcgctttt ttaaactgag ctcaggctaa aagagtccac tggactcttt tactccgtcc
      181 cataaccaat gatttaatgg cgcgttcagc tagattattg gaaaggacca gatgtccgtc
      241 ttttaaaatg gtcttaaagg tttctttata cttgaggctg tattcaattg cccttcctag
      301 ttttgaacct gctaaaactg actgacggcg gcaccaagca aagaagtctt ccattagggg
      361 ctggacgttt ctgtagtcgt tcatcagctg gcaaagcctc ccagtctctt tccaaggcaa
      421 ataactggtc acgataggct aaacctttag ctcctaagga tgatttatct gcttgcttgg
      481 gggtcgcttc aaaaaatttc cttctcacat gcgcccaaca tccaacaagt tcagcttctt
      541 ccagttgacg ataagcttct agcggttcgc agtttaccaa gcttagcgtc gccttgctcc
      601 taaaccttag actgctatcg cataggcaga actagaggac taaagttcta agttactgcc
      661 gcaacatatc acaatgcata tagccagaat aatttcctag gaattcttgc actaccgaac
      721 cactccgacg ctgatcatgg tggtacagcg taatcccttg tttctcagct ttcccagaca
      781 aaaaagtcca atagtaagtc agctgactat cactttctaa gacccgataa gaggtttcat
      841 ctgcatgaag aagagcttgt tctaacaact tttctcgtaa aaggttataa agggactcta
      901 aatagtattg actcgccttg atatgccaat tagcaatttc cttacgtgtg attggtaaac
      961 ccatcttagc ccaatcttct tcttagcgat aattgggtac cttcagaata aacttctgat
     1021 ggatggtgtg agcgataata gaagctgagc caaggctatg cgccaaaggg gctttaggaa
     1081 taggagcttt cacgatttta tcacttggat tattcttact gcatgtttgg catttgtaag
     1141 cgtgttggat atgattcact ctttttaatt gcgcaagaat aaagaccaat tcttgtcgtt
     1201 gaaggctcgc tccaatctct tttagctctc cctgacagtc agggcaaatg cagttctcta
     1261 agcgatgatg aacttcttct gaatcaaatt gggcaagaat agcttgacgt ttccctttag
     1321 ctttcttacg tttataggtg atttcttctc tttcaactgg gtaagtcaga gtcttcttcc
     1381 atattttgtt cctcttcaaa aagactgagt tgtccagatg ggcaaacact tttctcagag
     1441 gattttccat agagcttttg cgttagataa gccacttgtt cacgaagaag ggcaagttca
     1501 ttggtgagac tatcaattgt agcactctgt tgttgaatga ttttcaatct tctcccatag
     1561 actttcctcc ttatttccta actttatt
//
LOCUS       DAVMGZ010000188         1539 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000188 DAVMGZ010000000
VERSION     DAVMGZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.189"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            374..595
                     /locus_tag="VYQ93_001952"
     CDS             374..595
                     /locus_tag="VYQ93_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336581.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            679..1482
                     /locus_tag="VYQ93_001953"
     CDS             679..1482
                     /locus_tag="VYQ93_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV3336582.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
BASE COUNT          490 a          320 c          290 g          439 t
ORIGIN      
        1 agtgtgaatt ttgcttaaaa tcattaaaaa acaccccatg gtgtgaacca tgaagtgttg
       61 taaatgctgt gttgtagcta gttcttagcg tttagagaac tggctagctt tacgagcttt
      121 cttaagatct ggtttccaaa atactttgag tacacttatg gtattgatgc ccctaaacat
      181 ctaaaaactc tggtagaaaa agggtatgct cttgtcgaaa cttcttttga ctcacttgac
      241 catctaaata ctaccatgaa aaagaatatc ctgaagaaga agggagttac tggactttct
      301 aaaatgaaat cttgcgacct ggatcaagcc cttcatgagc atttttcaga agaagaatta
      361 gctggtcact ttcatgtcct tctatggact tttttacaat ggcattgcta tcacacccaa
      421 tacctatcta agcgcctggt tcgtaaactt tattgcagct cttcctctaa atttcctaat
      481 tgttgaacca attgcccgtt ttatactaag ttcttttcag aaaccattta ctggggaaga
      541 agttgaagaa gttgaagatt ttcaagatga tgatgaaatc ccaactatta tctaagccag
      601 ttctgtaaac tactaatatt tgaaatccac ttccttttag ggtacaatgg ttataaatga
      661 atttttgaga ggatcagaat gaaaaaacta gcaacccttc ttttactgtc tactgtagcc
      721 ctagctgggt gtagcagcgt ccaacgcagt ctgcgtggtg atgattatgt tgattccagt
      781 cttgctgctg aagaaagttc caaagtagct gcccaatctg ccaaggagtt aaacgatgct
      841 ttaacaaacg aaaacgccaa tttcccacaa ctatctaagg aagttgctga agatgaagcc
      901 gaagtgattc tccacacaag ccaaggtgat attcgcatta aactcttccc taaactcgct
      961 cctctagcgg ttgaaaattt cctcactcac gccaaagaag gctactataa cggtattacc
     1021 ttccaccgtg tcatcgatgg ctttatggtc caaactggag atccaaaagg ggacggtaca
     1081 ggtggtcagt ccatatggca tgacaaggat aagactaaag acaaaggaac tggtttcaag
     1141 aacgagatta ctccttattt gtataacatc cgtggtgctc ttgctatggc taatactggt
     1201 caaccaaaca ccaatggcag ccagttcttc atcaaccaaa actctacaga tacctcttct
     1261 aaactcccta caagcaagta tccacagaaa attatcgaag cctacaaaga aggtggaaac
     1321 cctagtctag atggcaaaca cccagtcttt ggtcaagtga ttgacggtat ggatgttgtg
     1381 gataagattg ctaaagccga aaaagatgaa aaagacaagc caactactgc tatcacaatc
     1441 gacagcatcg aagtggtgaa agactacgat tttaaatctt aaaaaccaaa aaaatacagt
     1501 atccacattc ggtactgtat ttctggctct ataatatct
//
LOCUS       DAVMGZ010000189         1538 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000189 DAVMGZ010000000
VERSION     DAVMGZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.190"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..108
                     /locus_tag="VYQ93_001954"
     CDS             <1..108
                     /locus_tag="VYQ93_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV3336583.1"
                     /translation="SFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            576..947
                     /locus_tag="VYQ93_001955"
     CDS             576..947
                     /locus_tag="VYQ93_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336584.1"
                     /translation="MIKKNHILCEIVPGSVVFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
BASE COUNT          473 a          199 c          275 g          591 t
ORIGIN      
        1 agttttatta ttggtttagt agttggtata tttggtcctt atatggatga cttaattaga
       61 aaaatatttt caaaatcttc caaaaaggat acggatagta ctctctaaaa aataaatttc
      121 tcccctgtat ttgctagctt aatttacagg ggagtttctt taatattgtt tcgcctgtca
      181 aaatgatatg tgatagcagg atgaaaagtc tacaaacaag ttggtatcaa tacaatataa
      241 agaggaaagt atgtttttca tgcttgcttt tttaattttt acaattcaag aggttttgat
      301 gaccatttat gatttgagtg atccaaggtc aaaataagtg ctatactagc agtgtaaatg
      361 ttcttgctca actaagataa atcgcacatt taggaggaaa acttatgaag aaaaaaatac
      421 tgattatttt cgttctgtat ctgatcatgt ccatctttct ttatccgctt agggagagta
      481 tttggtataa tctattttat accatagcct atatgattgc ggttatgatc tatttttctt
      541 taattaaaaa gaaagaaaag aaatgaaaga ttttcttgat taaaaagaat catattcttt
      601 gtgagattgt tcctgggtca gttgtctttg cttgtttcta cttatctatt tctatttcgt
      661 cagttttcca atttctttta gtagttatta acttgacttc tattttggtt actgtttatc
      721 ttactaggga aatgagatta agagagtttg aagatgatga tttggttagt cctagaacca
      781 atcaactcat gtttatcggc ttgacaggct ttatgtctat tatttgtttg tatagaggta
      841 tcacagcagg agaatcttat caacaactaa tcgcttatat tggtgctatt ctttgcttga
      901 tcatcatgct tctactcatt tggggtttga agtattataa aaagtagggg ctaatgaagt
      961 ttttgcaatt taggaggttt cgatgacaat tcaagatttg gatgaaaaat tttaaaaaac
     1021 aatgatatac taaacttgtc aaagttgcaa cgggtgctgc tacattatgt taaaaatcgc
     1081 tcttgattga taagttggat ttgttaggaa aggagtaaat aattatggat aagaaaaaga
     1141 tagtttcgac tataatatgt atagtatttc ttgtagtgtc agtagataat ttttttagag
     1201 acttaacccc gttactgttc attttaaata ttattggcct gtcttgcttt tcagtgctaa
     1261 catacattaa cataaaagag atactcttaa atataagtaa atgatgattt ggttagtcct
     1321 agaaccaatc aacttatgtt tatcggcttg gcagatttta tgtttgtcat ttgtttgtat
     1381 agaggtattt cagaaacaga attctatcaa caactaatcg cttatatagg tgtattctct
     1441 gcttgtttat cacgcttctg ctcatgtggg gcttgaagta ttataaaaag tagctttcta
     1501 tccttttctg acaggatatt tgagtagaag gctctatt
//
LOCUS       DAVMGZ010000190         1489 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000190 DAVMGZ010000000
VERSION     DAVMGZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.191"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            28..1374
                     /gene="gdhA"
                     /locus_tag="VYQ93_001956"
     CDS             28..1374
                     /gene="gdhA"
                     /locus_tag="VYQ93_001956"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV3336585.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          457 a          293 c          319 g          420 t
ORIGIN      
        1 aaaatatgac aataaaagga gaacgatatg acatctgcta aagaatatat ccaaagcgtg
       61 tttgaaactg tgaaagctcg taacgggcac gaggctgaat tcctccaagc tgttgaagaa
      121 tttttcaaca ctttagaacc tgtatttgaa aaacaccctg agtatatcga agaaaatatc
      181 ttggcacgta ttactgagcc tgagcgcgtg gtttctttcc gtgttccttg ggttgaccgt
      241 gatggaaaag ttcaagtaaa ccgcggttac cgtgttcaat tcaactcagc tgttggacca
      301 tacaaaggcg gacttcgttt ccacccaact gtaaaccaag ggatcttgaa attcctcgga
      361 tttgaacaaa tctttaaaaa tgtcttgact ggacttccta tcggtggagg taaaggtgga
      421 tcagacttcg atcctaaagg taaaacagat gctgaagtga tgcgcttctg ccaaagcttc
      481 atgactgaat tgcaaaaaca catcggacca tcacttgacg tacctgctgg tgatatcggt
      541 gttggtggac gtgaaattgg ttacctttac ggtcaataca aacgtcttaa ccaatttgat
      601 gctggtgtct tgactggtaa acctcttgga tttggtggta gcttgattcg tccagaagca
      661 actggttacg gtttggttta ctatactgaa gaaatgctca aagctaacgg taacagcttt
      721 gctggtaaga aagtcgttat ttcaggttct ggtaacgttg ctcaatatgc tcttcaaaaa
      781 gcaactgaac tcggtgcaac tgttatttct gtttctgact caaatggtta tgtcatcgat
      841 gaaaatggta tcgacttcga tcttttggtt gatgttaaag aaaaacgtcg tgctcgtttg
      901 actgagtatg cagctgaaaa agcaactgca acctaccacg aaggtactgt atggacttac
      961 gctggaaact atgacattgc tcttccatgt gccactcaaa acgaaatcaa cggtgaagca
     1021 gctaaacgtt tggttgctca aggcgttatc tgtgtatctg aaggtgccaa catgccaagc
     1081 gaccttgatg ccatcaaagt ttacaaagaa aatggtatct tctacggacc tgcaaaagct
     1141 gccaacgctg gtggtgtagc cgtttcagct cttgaaatga gccaaaacag tcttcgcctc
     1201 tcatggactc gtgaagaagt tgatggccgt ctcaaagaca tcatgacaaa catcttcaac
     1261 acagctaaaa cgacttcaga aacatacggt cttgataaag actaccttgc aggagctaac
     1321 attgctgcct ttgaaaatgt agcaaatgcc atgattgcac aaggtattgt ttaagattta
     1381 tttactcaat cctcaatcac tacgattggg gatttttagc ttgaattaaa aaattggaaa
     1441 atctaattaa ataaaatgaa atgcaataaa aactgaacaa attgattgc
//
LOCUS       DAVMGZ010000191         1473 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000191 DAVMGZ010000000
VERSION     DAVMGZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.192"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..734)
                     /locus_tag="VYQ93_001957"
     CDS             complement(<1..734)
                     /locus_tag="VYQ93_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336586.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFVQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYVKEISSARYSEYLTCEYKNLTPIILIAI
                     FENLLDSYLLQNQSEVDLDIFYKVLP"
     gene            complement(891..1136)
                     /locus_tag="VYQ93_001958"
     CDS             complement(891..1136)
                     /locus_tag="VYQ93_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336587.1"
                     /translation="MGIYGRKTIAMKHFFAGIGEIHFLLFLLYLSMSASPIIGLVIGY
                     TKINLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
BASE COUNT          564 a          262 c          186 g          461 t
ORIGIN      
        1 aaaggtaaaa ctttataaaa aatatctaag tcaacctcac tctgattctg aagcaagtag
       61 gagtcaagca aattttcaaa aatagctatc aatatgattg gtgtaaggtt tttgtattca
      121 catgtcaagt attctgagta tcgtgcagat gatatttctt ttacataact ttttgaaagc
      181 aagatttttt caaattcatc atctattatt aatcttctat ttccaatcca agcggtatta
      241 ccacccaaat aagattgtac aaatagcaat atgtaagaaa atatgaacgt aaatactgaa
      301 agaggtagaa ttctatgcgt aaaaaaatag acgataatgg tagccaggag taaagatatc
      361 tctcgtgaca atcgaattct tttcagcatc ttttttaaca gtccgtaagg ttgtccttgg
      421 caatattctt gaactaaatt aatcggaatg acatctctca ccatttctgg aaagtagagt
      481 aagtttatag gttgaaatct ccaactccca tcataagtaa atggatatag aaccaaatac
      541 ttaaaagatt tttttcttat aaacttaatt atcacaatat gaatgactgt tgcaatgaag
      601 tatattaaat ataaaagtaa aaccgccatc aatatactta caaaaatccc cttggactca
      661 atcaatggaa aataaaatat tgcaactaga agcgaataaa gtcccgctaa taaatatttc
      721 tgatcatctt tcattcaaaa caccatatta tttactaaga atacatcaat acaatagagc
      781 aactctgtaa gtcaatattt gataaagcaa agctttaacc ccttccccag acgttagctt
      841 ttcttcaacc cactacagtt gacaaggagc ccaaattatt gataaaaact ttagtcatca
      901 tcgtctctat aaaaatattt tctatacact cttgataata ccaaaattaa tgccaatatc
      961 ataccaagag ctataataat agaatttatt agatttattt tagtatatcc aatcactagt
     1021 ccaataattg gactagcaga catgcttaga tataataaaa ataacaaaaa atgaatttca
     1081 ccaattcctg caaaaaaatg tttcatagct atagttttac gcccataaat acccatcaaa
     1141 tttaccgtct acgatatcta aaccatatgc aatggctcct cctaatgaga agacttcagc
     1201 aacagtagcc gcaatattaa gagcattagt aatagttgcc atactaactc caccaatata
     1261 tcttgttata gcacttagtt acctagataa gatatccttt agcttgcttc caaaatttaa
     1321 gtctacacct ataatatgag ttcatattag tactgctaaa tcctatctat agtaatgtca
     1381 aacacctaaa taaagtcatc tctccaattc attcaagaaa tgaagaaagg caaaaaatct
     1441 ttaaaataag aaaagtgcat attatcaggt gtt
//
LOCUS       DAVMGZ010000192         1461 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000192 DAVMGZ010000000
VERSION     DAVMGZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.193"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            101..1279
                     /locus_tag="VYQ93_001959"
     CDS             101..1279
                     /locus_tag="VYQ93_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV3336588.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQIILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          544 a          264 c          212 g          441 t
ORIGIN      
        1 ccagtaagta atgaaaagct taaaaagatt aacatctaca tttatccata gcaaaaaatc
       61 ataggatttt aagaatctga caactaggaa gaaaactaat atgaccttat ctaagaaaca
      121 attacaacta agagctaaaa tactggaaac agtttacaca ttaggtccca tttcacgaat
      181 tgaaatcgct actaaaacag gaataacacc tgcaacaact agcagtatca caaatgatct
      241 gattaaagaa aatattctct tagaacttgg tgaagacgaa cacgatacaa gcgtagggag
      301 aaaaaaaatc ctgctagaca ttcaagctaa acgattttac tacattggtt gtgaactatc
      361 tgaaaaacat ttcacttttg ctttaggaga taatctagga aatatcttaa aggaagagaa
      421 agaaattgtt actaaacagt taatccaaga aaagggaaat caactcatta accagacact
      481 aaagcaattc ttaaataact gctctgatta tgaaatagaa gcaattggaa tcgcgctacc
      541 tggtcgctat ttagataatt ataaaataac aacaaacaat cctttatggc aacatatcga
      601 cttagaaatg attcaatccc actttgataa gcctttgttt ttctctaaca atgttaactg
      661 tatggctata ggtaaacgcc tatttagtcg ccaacagaat gaccctaatt ttgcttattt
      721 tcattttgct agaggtatgc actgttctta tatctacgac ggaaacattt atgggaaagg
      781 aaatctaatg attggagaaa tagggcatac agttgtttca tctgagggag aagaatgtag
      841 ctgcgggcgc aagggatgct tacaaacttt tgctggagaa tcttggttaa tcaaaaaatc
      901 aaaaattcta tatcatcaat ctccctattc tctacttcct agtttagtca aaaatgccga
      961 tgatattgat atccaaatta ttttaaccgc ttatcaatta ggtgacactg gaataatcac
     1021 acttatccat caagccttac tctatcttag tcaaaccatt ttaaatatca gtatgatgat
     1081 tgactctcaa aaaatctacc tacatagtcc tttactaaca aatcaacaca tcatccaaaa
     1141 actatatagt gaaatgaact acaaacccaa actgctctat aatcgcctac ctgaagtcat
     1201 tattgaacct tacaatgatt ttacagccgc acactcagct attgcacttt gtttgtatca
     1261 tacaattcta cattcttaat tttctattac atttaataaa taggctaaaa agagggagta
     1321 tatcataaaa agcgaataaa cctgttttct gaaaatcaga attctgtttt gttcgctttc
     1381 tttatatttg gaattatact cctatctccc ctttttcata atgtataata aaatgaaata
     1441 ataacaggac gaatcgatcg g
//
LOCUS       DAVMGZ010000193         1427 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000193 DAVMGZ010000000
VERSION     DAVMGZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.194"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(5..337)
                     /locus_tag="VYQ93_001960"
     CDS             complement(5..337)
                     /locus_tag="VYQ93_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV3336589.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICVALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGKIAYCIGYGALNRGCDISGNPYPWD
                     GFISATVR"
     gene            complement(393..1295)
                     /locus_tag="VYQ93_001961"
     CDS             complement(393..1295)
                     /locus_tag="VYQ93_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336590.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHLLVSNLQLNLEIPLDFVVSTSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTKTLKDKIQKSELSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEMSISNNNNWSNKVDKIRIHLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
BASE COUNT          507 a          265 c          183 g          472 t
ORIGIN      
        1 ttattcatct tactgtcgca gatataaatc catcccaagg ataagggttc ccgctaatat
       61 cacaacctct attaagagca ccatatccaa tacagtaagc tatttttcct gctgctgcac
      121 caataagcca gcctccaaga cctccaacct taatatagtt tattagtttt ttggttaaaa
      181 ctgcagctcc agctaatgct ataaaagttc ctgaactcat agctaaagct acgcaaattc
      241 cttgacaagc acgcttgctt aaataagctc ccccatcaag atacatcatt tcttcttcat
      301 caatcacaac ataattattt ggtaatacaa gttccatttg ttttctcctc cttagttgaa
      361 attgtttcat ttgttttaca aataggacga acttaaacta tctttttatt actcagtaat
      421 aatttccgag atttcatttt atgatactct ctattcatac agaaatcaaa attttgcctt
      481 aaccaacttg tataatagaa aaaatctaat tgattgattc tgatgaggtg tatcctaatt
      541 ttatcaacct tgttggacca gttgttattg ttactaatag acatttcata cagcagtaac
      601 tcgacaagac ctgtacacag aagggtgtta tgagaatctt cgattttttt caaaatatcc
      661 tcaaaaagtt cactatccgt taaaaaggtg gattggttca tcgcatcata cagctgaaga
      721 gacattaagt cagaataact tagctctgat ttttgtatct tatcctttaa cgttttcgtc
      781 aaatcgcttt caaacgaatc ttctttttcc catttatttt gattaaaaag aaattccgtc
      841 ttattaaaaa cgagatgatt gatatccatt atagtccaga aaaatattgt agtaagcatg
      901 atgattgcaa aaggaatttt taaaataaat actagaataa tagcaatttc aagaataaaa
      961 ctccatataa tcctagatct taaatcatca ggcgacaaac ttttacgatg gaatgaaaaa
     1021 tagggcgaga acctcacatt tactttcttc ctgcctataa aaataaaagg aaaaatcgta
     1081 ataagagctt ctttcttgta caaatctttc aatgcgtata gtatgtctaa aactattttt
     1141 gataaaatca aactacagaa agcaagactc gtactaacca caaaatcaag gggaatttct
     1201 agattcaatt gcaagttact cactaacaaa tgcaactcta gccacaagac aaaaagaggg
     1261 agcattaata aaaaaatgat tagtttacgt atcataattt tacagtgtcc ttatcatttt
     1321 cttaattttg gataaattat cattgctatg attaaaatca aaaaaacgac attcatccaa
     1381 ataatcaact tttttatttc ttcgctaaca aaatcatagg ctaaaaa
//
LOCUS       DAVMGZ010000194         1413 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000194 DAVMGZ010000000
VERSION     DAVMGZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.195"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1137..1376
                     /locus_tag="VYQ93_001962"
     CDS             1137..1376
                     /locus_tag="VYQ93_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336591.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
BASE COUNT          457 a          189 c          269 g          498 t
ORIGIN      
        1 gtaatagaaa cataggttaa atcttagaac ggcttaaaat cagatatgat aaagggaaat
       61 agtcttatct aggtaactaa gtgctacaaa cacagtcgag gatcacttgt taggattaca
      121 ttttaatcat gcttaataag aatggagaat tcctatgggg attgtattac tctgaagttt
      181 cgaatacctt agttctcgtg attttgagct gttctagctt tagaatcctt cagaatgtaa
      241 aattttaagc agtcaaagac ttagaatagc ctggaaacat ttattttata gcaattctag
      301 gcttagaatt gctcatatat atgtatttga gctgctcgat agttaggaca gctctattct
      361 gagaaatctg agctatcaat gcttcagaat agtatttcgc aaatggtttt tattgaagag
      421 gtgattgttt tatgatagtc taaaataaaa ctgatttaat ggagtaaatg gagaatcgtc
      481 acatgaaaca tttttttgca ggaattggtg agataaaact tgttggctac ttattgtata
      541 tatgtgtagg actagccctt ctttttcata tatatgtcat aggttcggaa ctgagttttg
      601 tgaaaatagt gttatctatt ttgggagtta tatttgtaag tatgttaact attgctcgaa
      661 tttatcggac ttttctataa agaatagttt ggacttgaac tgtatctaaa aacagaggtt
      721 ggtcgattat cagcctcttt cttgtcgttg aaaagagtga gatatattga cttttgaaaa
      781 aaatgtcata attgagttgg gataaaccta aaagtaatat agtagattga atctggaata
      841 gtccgttatg actgttaaaa tatttctaga aattaatttg actttcctaa tcgatttgtt
      901 cacatcttgt ttcaatctac tatactttct cgttaaaccg ttcggcaatt catggtgaca
      961 tggacacata gtagtaagca cactatgtgg cttggcagag ctaaaaactg ttcccttgcg
     1021 ataagcctaa taagcacaac atagggagtt agagaagccg actctaatca accactttgg
     1081 gcagtagtga gaactgcccc gtgctttttt attttgagaa aatatggagt ttgtcgttga
     1141 aattacttga ttgtattttg gactatcaag aaagattcaa tggaaaaaca tgtcaagtat
     1201 caacgaatta taagtattta gagattttca aagtgaattt ttgcttgact gatttacatc
     1261 atttatttga cttatacaaa atcacacgag attatgctag tcaaacaaaa cctgctattc
     1321 aagctggtgt ttttatttta gaagatttta gaaatatcct atgtacaatg atgtaatcga
     1381 aaggatatct ttatatgagt ttatcggtga tat
//
LOCUS       DAVMGZ010000195         1390 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000195 DAVMGZ010000000
VERSION     DAVMGZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.196"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(326..1219)
                     /locus_tag="VYQ93_001963"
     CDS             complement(326..1219)
                     /locus_tag="VYQ93_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3336592.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
BASE COUNT          464 a          283 c          160 g          483 t
ORIGIN      
        1 aacttttata cgattactac ctctttttag tttacacttc ctacatcata aataaacata
       61 ctcaaccctc acaatctcca aataactctt ctatatcatt gaaaattgag tttcatcctc
      121 tatactaatg taggacattc ctttaatcca atataagaat accttgttcc attaatctct
      181 acccgatatt atagatagaa ccaaattcct atacttaaaa ggacaaataa cgttccaacc
      241 ataataaacc tcctttcttt ttgagaacgt tttctttata ggtttagtat agcaaaaaca
      301 tgaaaaaaac aacatgactt agaagtcagg ttgttgggaa atctatctgt ttttctgcta
      361 aaattctctg aactaatacc tgatcgtcta gcaattctgc tagagaaatc gccttttcat
      421 agaaactttc tgcctctaaa atattcttgt ctacaaataa agcatatttt gcctttagca
      481 aaaatagatg aggtcggtaa aaaacatgtc ttgttttagc aataattttc tcaaatgttt
      541 gaatatatat cagactatct tctgtctgaa tttggataaa taggctcaga agaacttttt
      601 ccatttgaac taggctagcc ttatcttcat attctgaaca tagcaatact ttttgagcta
      661 actcctcaaa atgttcttta tcttctaatc ccatagcaca acaaaagaaa tacaattcaa
      721 taatcaacaa atcatttaca gaaaaatatt tcttttttaa taactgctgg aaatattctt
      781 ccactaaccc taaaccgtag gtcacatctg aactaccata aatatcaaaa cgagcttgtg
      841 tcacatccac aattaactgc tcttcctcag gcaaattctc ataatagttt tcatatattt
      901 cctcaagtat agcttctttt cgttcgattc ttcccttatc agcgtaggtt tgagaatgaa
      961 tcagctcatc tttgagtttt aggaatcctt ttggaatctc tataacctta acatccacaa
     1021 tattctgtaa tgatactcca agtttatccg caatcaataa taatttagga agtgttgcta
     1081 aagcttgccc attttcaata cgtgctagct gacgaatcgt caactctgat tcatcaccac
     1141 aaatatctat ctgagttttt ccacaagatc gcctaatagt tttgatttta taaccaattt
     1201 cacgtttcaa ctcatccata gtaatactcc ttaagttgat tacaaagatt tctttttctt
     1261 cctaattctt ttgtcaatca tatataaaac gataagcgaa aacagcggta gaacaatata
     1321 actcttttca ctcattatca gctgaaatcc cagggttgct agtatcttaa agatgaaaat
     1381 attcatcaaa
//
LOCUS       DAVMGZ010000196         1365 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000196 DAVMGZ010000000
VERSION     DAVMGZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.197"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>1365
                     /gene="phtB"
                     /locus_tag="VYQ93_001964"
     CDS             <1..>1365
                     /gene="phtB"
                     /locus_tag="VYQ93_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV3336593.1"
                     /translation="YQAGQDKKESNRVAYIDGDQAGQKAENLTPDEVSKREGINAEQI
                     VIKITDQGYVTSHGDHYHYYNGKVPYDAIISEELLMKDPNYQLKDSDIVNEIKGGYVI
                     KVNGKYYVYLKDAAHADNIRTKEEIKRQKQERSHNHNSRADNAVAAARAQGRYTTDDG
                     YIFNASDIIEDTGDAYIVPHGDHYHYIPKNELSASELAAAEAYWNGKQGSRPSSSSSY
                     NANPAQPRLSENHNLTVTPTYHQNQGENISSLLRELYAKPLSERHVESDGLIFDPAQI
                     TSRTARGVAVPHGNHYHFIPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQPT
                     PEPSPSPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSK
                     LAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDLLDNKGRQVDFEALDNLLE
                     RLKDV"
BASE COUNT          444 a          280 c          293 g          348 t
ORIGIN      
        1 taccaagctg gtcaggataa gaaagagtct aatcgagttg cttatataga tggtgatcag
       61 gctggtcaaa aggcagaaaa cttgacacca gatgaagtca gtaagaggga ggggatcaac
      121 gccgaacaaa ttgttatcaa gattacggat caaggttatg tgacctctca tggagaccat
      181 tatcattact ataatggcaa ggttccttat gatgccatca tcagtgaaga gctcctcatg
      241 aaagatccga attatcagtt gaaggattca gacattgtca atgaaatcaa gggtggttat
      301 gtcattaagg taaacggtaa atactatgtt taccttaagg atgcagctca tgcggataat
      361 attcggacaa aagaagagat taaacgtcag aagcaggaac gcagtcataa tcataactca
      421 agagcagata atgctgttgc tgcagccaga gcccaaggac gttatacaac ggatgatggg
      481 tatatcttca atgcatctga tatcattgag gacacgggtg atgcttatat cgttcctcac
      541 ggcgaccatt accattacat tcctaagaat gagttatcag ctagcgagtt agctgctgca
      601 gaagcctatt ggaatgggaa gcagggatct cgtccttctt caagttctag ttataatgca
      661 aatccagctc aaccaagatt gtcagagaac cacaatctga ctgtcactcc aacttatcat
      721 caaaatcaag gggaaaacat ttcaagcctt ttacgtgaat tgtatgctaa acccttatca
      781 gaacgccatg tggaatctga tggccttatt ttcgacccag cgcaaatcac aagtcgaacc
      841 gccagaggtg tagctgtccc tcatggtaac cattaccact ttatccctta tgaacaaatg
      901 tctgaattgg aaaaacgaat tgctcgtatt attccccttc gttatcgttc aaaccattgg
      961 gtaccagatt caagaccaga acaaccaagt ccacaaccga ctccagaacc tagtccaagt
     1021 ccgcaaccag ctccaagcaa tccaattgat gagaaattgg tcaaagaagc tgttcgaaaa
     1081 gtaggcgatg gttatgtctt tgaggagaat ggagtttctc gttatatccc agccaaggat
     1141 ctttcagcag aaacagcagc aggcattgat agcaaactgg ccaagcagga aagtttatct
     1201 cataagctag gagctaagaa aactgacctc ccatctagtg atagagaatt ttacaataag
     1261 gcttatgact tactagcaag aattcaccaa gatttacttg ataataaagg tcgacaagtt
     1321 gattttgagg ctttggataa cctgttggaa cgactcaagg atgtc
//
LOCUS       DAVMGZ010000197         1354 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000197 DAVMGZ010000000
VERSION     DAVMGZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.198"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            30..869
                     /locus_tag="VYQ93_001965"
     CDS             30..869
                     /locus_tag="VYQ93_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV3336594.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            976..1251
                     /locus_tag="VYQ93_001966"
     CDS             976..1251
                     /locus_tag="VYQ93_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV3336595.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
BASE COUNT          447 a          211 c          291 g          405 t
ORIGIN      
        1 ctgaatccta aaatagaaag attgatacta tgacaaaaat taagattgta accgattcat
       61 ctgttactat tgaacctgaa ctagtaaagc aattagatat tacaattgtt ccattatctg
      121 taatgattga taatgttgtt tattctgatg cggatttgaa agaagaaggt aaatttcttc
      181 agttgatgca agaaagtaag aatcttccga aaacaagtca gccacctgta ggtgtctttg
      241 ctgagatttt tgaagaccta tgcaaagatg gtggccagat tcttgctatt catatgtccc
      301 atgctctttc gggtacggta gaagcagcac gccaaggtgc tagcctatct actgcagatg
      361 tgactgttgt tgatagttcc ttcactgacc aagccctgaa attccaagtt gttgaggctg
      421 cgaagttagc tcaagaaggt aaagatatgg aggcaatttt atctcatgta gaagaggtta
      481 aaaaccatac agagctttat attggtgttt caactttgga aaatcttgtc aaaggtggac
      541 gaattagccg tgtaactggc ttgttgagct ctcttctcaa tatccgtgtt gtcatgcaaa
      601 tgaaagacca tgaattgcag ccaatggtta aaggtcgtgg aactaaaaca tttaaaaaat
      661 ggttagatga gttgataaca tcgctttctg aacgtgctgt agcagagatt ggaatttcat
      721 attctggtag tgatgattgg gcaaaagaga tgaaagaaag cttacaagct tatgttgaaa
      781 agccaatttc tgttttggaa acgggttcca ttattcaaac tcacacgggt gagaacgctt
      841 gggctatttt gatacgttac cactcctaaa aaaataaata aaatgggaag aaatagcgtt
      901 tttaacttga cctaaaaggg attttaggat atgattatat ttgttaatta gaaattattt
      961 ggaggaatca ttaacatggc aaacaaacaa gatttgatcg ctaaagtagc agaagctaca
     1021 gaattgacta agaaagactc agcagcagca gttgaagctg tatttgcagc agtagctgac
     1081 tatcttgcag ctggtgaaaa agttcagttg atcggttttg gtaactttga agttcgtgag
     1141 cgcgcagaac gtaaaggtcg caacccacaa actggtaaag aaatgacaat tgcagcttct
     1201 aaagtaccag cattcaaagc tggtaaagct cttaaagacg ctgttaaata atcagtcttt
     1261 aaaaagccta ttgtatcaag ctttctagct tggtcagtag gctttttaat tatgaaatct
     1321 agtaataaat agagttaaaa agttgaatgt tccg
//
LOCUS       DAVMGZ010000198         1342 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000198 DAVMGZ010000000
VERSION     DAVMGZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.199"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(200..1309)
                     /locus_tag="VYQ93_001967"
     CDS             complement(200..1309)
                     /locus_tag="VYQ93_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV3336596.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT          500 a          249 c          198 g          395 t
ORIGIN      
        1 aggtttcatt cgaactttaa tagactatga aaaccagtgg aacttaccct gacacagaat
       61 tccactggtt ttttcgattt ttatcagatt aacttctact attcttagct gtgaaaatta
      121 gtttaagact ttaacttatt ttcagtcttc taatctaaag gccacttcca gtaaagaaaa
      181 atcagggttc tcctgacttt tacttagcta attctctttc tcgttctttc attattttat
      241 gattttcata caaacgggat aagaacttag aaatttcttt caagatagaa taggttggca
      301 ctgcgacaat cattccaact acaccataga tattgcttga caacaaaagt aaaactaaaa
      361 tcgtgattgg atgaaccttc ataacacttc ctacgattcg aggatataag atattgccat
      421 ctacctgctg aacaacaagc atataaatca ctgcaatcag cagtctatgg ggatcagtga
      481 atatatttgc gatgatcata ggaatcaaac caatacttgg ccccacataa ggaattaaat
      541 tggctacacc agaaaaaatg gcaaaaacta aagcatattt taaaccaata atactatagc
      601 caatataagc caaacaacct ataatgattg cgtcaatcga aactccacta atatagcgag
      661 caatcgtcgc atttaaattc tttaataagc ctgcaatatg caagcgatcc ctctttagaa
      721 tcgttctttc aagcatgggc aagaatttat gtccatctaa taagaaataa accaaaaaaa
      781 ctggagtcat aatcaaaatc aaaacagtac tgataagagc tgacaagacg ctccccacac
      841 tatttgatac gctatttagg atattttgaa gaatatcaac ataggataag tttaactgct
      901 gaattgtagc ttctacatcc aaattctgga gcgcaggata attagataag tctatgatta
      961 agtcttgtac tcgactataa atagtttgac tagatataat caaactagat aactgattaa
     1021 tcaaaatagg taagagatag acaacaccta tgaccattcc ccagaccaaa gtacacaagg
     1081 taattaaaat accaagcaaa cgattgagtt tacagacttt atttaagaaa gtaacaatag
     1141 ggtttgtcaa ataataaaga aagcccccta ataaaaatgg aatcataatt gtattaagca
     1201 cgctaacaaa agggttaatc aaagacccca tctgtctcca taggtaaaag atgatggtta
     1261 agagtaaaat ttctgtggtc caaaaaaata atttatttct acgaaacatg agttacctcc
     1321 ctcactttat tttaccatat tt
//
LOCUS       DAVMGZ010000199         1325 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000199 DAVMGZ010000000
VERSION     DAVMGZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.200"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..245
                     /locus_tag="VYQ93_001968"
     CDS             <1..245
                     /locus_tag="VYQ93_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336597.1"
                     /translation="IIYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVI
                     NEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG"
     gene            560..>1325
                     /locus_tag="VYQ93_001969"
     CDS             560..>1325
                     /locus_tag="VYQ93_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336598.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLISFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINNPISLYGMLDVIKL"
BASE COUNT          446 a          178 c          219 g          482 t
ORIGIN      
        1 gaatcattta cgataatgaa gcattaagag atttgtcaaa acaatattta aacaaaaata
       61 tttcggaatt acaagatggt tctttcgagg gcggtataca agatgctgtt gcctctaaac
      121 aaatagtagt tataaatgaa tttatagctt gtttaaattc cagatgcgtg ccatcccaat
      181 atgatagatt tttttataaa gatagacctt atattttttc cagaaagagt cctattaagg
      241 ggtgaaaatt tgttacattt ggattaacaa gtgtcctagg gattagaaat aactctagta
      301 tagctagata tttttgagta actagaaata tcattaggct acccatatga ttgccagaaa
      361 agatggctga tgaatcttat ttatactgtt gcagtaggca ttgtctagga taaataagct
      421 atcttgatgg gggaaattgg aataatttta gtagaaacat taaaggatta tggataagta
      481 gcgcaggatt aggtgatgca atgtgctaag ttggcttggt gttagcagta gtattattta
      541 acaatggaaa gtagactaaa tgaaaaataa acgttatttt tttgacacaa ttttaataat
      601 tttactgctt ataagcacga tattttgtgt cagcccagta tttataaaat tggacatctt
      661 aggaactccc tctcatgcca tcttaacctt tgtattggcg ataccgttat tctatatttt
      721 gtctcaatgc ttacatactt tgctattact agtttcctca atcttttgta aattaagacc
      781 aatttatttt tattttatat ttgtgatcat tataggagct aggaaatact atcgaattct
      841 ctttcaccaa ctaatgggat ttagtccagg aatagcagtg ttttataagg agagtcaaac
      901 aactaagaat ctgtttaaat tttactactt cttgtacttt acaacactaa tttcatacta
      961 ttttttcttt acttttgtgt atgacaaacc gttgcttttg cctcttattt cattttctat
     1021 tatcatagca ttagtacaaa aactttatag aattgagaat caacaattat ttctgttaaa
     1081 atcaaaggta ctaacaatac tggaatctaa gaagaattgt gagtttaatc ttcaggatta
     1141 tcatgaaatt tggaaattgc aatcaaaatc tgagttgcca tgcgtggctt tatcttatat
     1201 cagtttaatc aagccatatc tttcagagag tgtgcgtgaa caaattgatt tattagaagt
     1261 taagcgattt aaaaaaataa ataatccaat atcactttat ggtatgttgg atgttataaa
     1321 attaa
//
LOCUS       DAVMGZ010000200         1309 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000200 DAVMGZ010000000
VERSION     DAVMGZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.201"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            21..>1309
                     /gene="pcpA"
                     /locus_tag="VYQ93_001970"
     CDS             21..>1309
                     /gene="pcpA"
                     /locus_tag="VYQ93_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV3336599.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKS"
BASE COUNT          508 a          185 c          211 g          405 t
ORIGIN      
        1 aaatatgtaa ggaaatttaa atgaaaaaaa ctacaatatt atcattaact acagctgcgg
       61 ttattttagc agcatatgtc cctaatgaac caatcctagc ggatactcct agttcggaag
      121 taatcaaaga gactaaagtt ggaagtatta ttcaacaaaa taatatcaaa tataaggttc
      181 taactgtaga aggtaacata ggaactgttc aagtgggtaa tggagttact cctgtagagt
      241 ttgaagctgg tcaagatgga aaaccattca cgattcctac aaaaatcaca gtaggtgata
      301 aagtatttac cgttactgaa gtagctagtc aagcttttag ttattatcca gatgaaacag
      361 gtagaattgt ctactatcct agctctatta ctatcccatc aagcataaaa aaaatacaaa
      421 aaaaaggctt ccatggaagt aaagctaaaa ctattatttt tgacaaaggc agtcagctgg
      481 agaaaattga agatagagct tttgattttt ctgaattaga agagattgaa ttgcctgcat
      541 ctctagaata tattggaaca agtgcatttt cttttagtca aaaattgaaa aagctaacct
      601 tttcctcaag ttcaaaatta gaattaatat cacatgaggc ttttgctaat ttatcaaatt
      661 tagagaaact aacattacca aaatcggtta aaacattagg aagtaatcta tttagactca
      721 ctactagctt aaaacatgtt gatgttgaag aaggaaatga atcgtttgcc tcagttgatg
      781 gtgttttgtt ttcaaaagat aaaactcaat taatttatta tccaagtcaa aaaaatgacg
      841 aaagttataa aacgcctaag gagacaaaag aacttgcatc atattcgttt aataaaaatt
      901 cttacttgaa aaaactcgaa ttgaatgaag gtttagaaaa aatcggtact tttgcatttg
      961 cggatgcgat taaacttgaa gaaattagct taccaaatag tttagaaact attgaacgtt
     1021 tagcctttta cggtaattta gaattaaaag aacttatatt accagataat gttaaaaatt
     1081 ttggtaaaca cgttatgaac ggtttaccaa aattaaaaag tttaacaatt ggtaataata
     1141 tcaactcatt gccgtccttc ttcctaagtg gcgtcttaga ttcattaaag gaaattcata
     1201 ttaagaataa aagtacagag ttttctgtga aaaaagatac atttgcaatt cctgaaactg
     1261 ttaagttcta tgtaacatca gaacatataa aagatgttct taaatcaaa
//
LOCUS       DAVMGZ010000201         1284 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000201 DAVMGZ010000000
VERSION     DAVMGZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.202"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(151..339)
                     /locus_tag="VYQ93_001971"
     CDS             complement(151..339)
                     /locus_tag="VYQ93_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336600.1"
                     /translation="MRLGGSLGQRRENEIFFGLLAILFIKPIIGIVKFFWMIISFAVQ
                     LLFYKILFKILDWLFKLI"
     gene            complement(299..481)
                     /locus_tag="VYQ93_001972"
     CDS             complement(299..481)
                     /locus_tag="VYQ93_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336601.1"
                     /translation="MNPIKAFDKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIRPEERE"
     gene            complement(483..1262)
                     /locus_tag="VYQ93_001973"
     CDS             complement(483..1262)
                     /locus_tag="VYQ93_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336602.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLCAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIIAGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
BASE COUNT          416 a          279 c          196 g          393 t
ORIGIN      
        1 aacatatata ctgcaaggtg aagctgacgt ggtttgaaga gattttcgaa gagtataaaa
       61 tgattttact gcttatatta taccatatgg agagagatta aaaaactagc agtggagttc
      121 ctgctagctc tctgcgactt ggattaccat ctagataagt ttaaagagcc aatccaatat
      181 cttaaataat atcttgtaaa acagcaattg aactgcaaag gaaatgatca tccaaaagaa
      241 tttcacaatc ccaataatcg gtttgataaa aagaatagca agaagaccaa aaaaaatttc
      301 attctctctc ctctggccta atgagccacc gagccgtatc cattagctta tctgcaataa
      361 aaagttttcc cagaccaaca acgacatggt cagctttctt tatcgttttc atcactttta
      421 caccttgcac ggtcaaaaag taattaccat aaattttatc aaaagctttg ataggattca
      481 tattactctc ctttaatgat ttcagcctct tttcggattg tttcaacatc ttcttgatat
      541 tgaacttcac tatagttgac tagcttggat aattctttct gcaagctttc ccccattggt
      601 ttcatagttg caaaggttaa accacctgca atgataccac ccaagatagg aataaatttc
      661 cctattcctt tggccagccc tcccttggta agattcacac caaatatttt taagactttt
      721 ttcaaaatag ggtaccaaag cgtctttgtt aaagctttat taggcactgt tttcattacc
      781 tgtttggcaa ttgttatacc accagcacat agcaaagcag cggttccatt cacccctaac
      841 attacgccta gataaagcaa gagggtattt tgagcatctt cactcaactc ctcgcgtgaa
      901 gcccaaagat cctcataacc ataaatataa cctaattctt gagccaattt cagagagaaa
      961 gcataaaatt gagccacatc agctggaatg gtaattgcca tagcaagccc tccagctaat
     1021 cctgccaaaa cagaagtccc actcgctaat aatacattgt cccgaataca agcattagct
     1081 acacgatcta atatttcttg agatagaaga gtcgttgtac cttgttccaa taaggtagga
     1141 atatcttttg gatccaattc tttggaaaac ttatccacta aaaatttcga acgatcaacc
     1201 ttaacaacag gtagtttcac cacttgttgc aatacttgca tagctaagtc ttgttcagcc
     1261 atatacaaac tccttatttc tttt
//
LOCUS       DAVMGZ010000202         1272 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000202 DAVMGZ010000000
VERSION     DAVMGZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.203"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(98..961)
                     /locus_tag="VYQ93_001974"
     CDS             complement(98..961)
                     /locus_tag="VYQ93_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV3336603.1"
                     /translation="MIEKMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYDIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLTSLFE
                     LRFQQYKELID"
BASE COUNT          383 a          255 c          236 g          398 t
ORIGIN      
        1 tattactaac aaggaggaat ataatagatg aagaaaagaa aaatccaact gatactccta
       61 cttatttcgg agtgggttat tgtcattcca tttttaacta atctataagt tctttatatt
      121 gctgaaaacg caattcaaaa agggatgtta attgtggatt ttctaatacc tgcagagatt
      181 ggataaagcg ttcaatctct ttttgattgc ttccctttgt ttgaagaaag acactcatct
      241 tctttaaaaa ttgccacgat actttttcaa aaacatcata cggtcgtaac atcctctcca
      301 actcggcttc gaagattggg atgtaggaga aaagttttcg ctccatgagt tctgataaga
      361 tatttaagag tccttgcttc atatacaatc gattgtgtac taactcttta aattctttgg
      421 atttttcgag taaggaggtt gataaaaaaa tcagatcttg attgctcaag aagggcatgg
      481 tattgcaaaa gagatagagt tcaaaccagg tccaagactc gatatcatag agataggtgg
      541 tcaaaaactc gctatcctcc tctgctagtg ggtagctttt atttagtgaa tggatggcat
      601 ctttaatcac gatggcattc aaacgacgat aggtctgcgc catctgttct tgatcgactt
      661 cctccaatag ctgctctaaa gcagctatat cctgatgggc aaagcgattc acaacctttc
      721 gaccgattcg catatgtgga gattcttgat agttgttgag cttgtgccca aactcatcaa
      781 aggtcacatt tataccttgg atagctagaa tcaacttatc cgcagacagc atagactgcc
      841 ctagttcaaa cttggacaac tgagaagctg ttagaccctc acaagccaca tctgtctgct
      901 taagctttct cgccaaacgt aattccttgt aaaattcccc cagttccatt ttttcaatca
      961 tcctaccacc tcctagcttt tgcatattat atcattattt gtgttcattt gtcaaaacat
     1021 tctgacactt tggtgattga aaaagccagc cttaagctga ctctaaaagg accgagcctt
     1081 gtacaataca gaactcaatc ctcgttctaa atagaatgtc taactttttt gggcagtaca
     1141 cgagcttggg gaacagccac tggaacgatg aggtgtgagc tcaaaatatc ctccagttat
     1201 gtttttccta atagtatacc ggaagagtga aaggatttta taatggagcg gttacaaaga
     1261 acctactttc ta
//
LOCUS       DAVMGZ010000203         1237 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000203 DAVMGZ010000000
VERSION     DAVMGZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.204"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(126..929)
                     /locus_tag="VYQ93_001975"
     CDS             complement(126..929)
                     /locus_tag="VYQ93_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV3336604.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT          400 a          243 c          207 g          387 t
ORIGIN      
        1 ttcggataaa ttaattgtct aacttttggg gggcagtaca tattcaacac ctgatactat
       61 gcgttttatt gtgggaatgt ttgcttcatt ttttcctaaa attcaatgtt tactcagtat
      121 ttggattatg aaaaatcgag gtctaaatct agatacattt tttctgaaga caaatcattt
      181 tgaccaccga gcaagagatt ttcaaaaaaa gctgttaaaa actcaggacg tcgctgtaaa
      241 atctttgcat tatctaatac caaggcatca cgaaaatatt tggaatgttg ctgaaatggt
      301 atattatcaa tatcaaaacc aaactcacga agatactgaa tcaaaaagac cgttactgtc
      361 cgagtgtttc cttcgcgaaa tggatgaatc tgccagattc ctgaaataaa atgctggatt
      421 tgtttaacca catccgcctg agttagtgtc gcatatgcaa cttgtttttc ctgattaaaa
      481 tcataatcta aggtcatttg aatcatggag taatcagagt acacaacact ttcaccattc
      541 aaaacaggtt cattctttgt gatattggtc tgacgaaatt gccccaccgg aatagagggt
      601 tcaaatatat cttgaaacaa ctccttatga atagcaagta aggtcgcagg actaaagcta
      661 aagcctcttc gagacaatag ttctacaata cgtagagaaa ccaagtctgc ctcttccgta
      721 cttgcatcaa tagtatggtg ataagccgtt gcatcctcat aaacctgctc ataagtcagt
      781 tctccccggg actgtttctc agccaaagat tccatatacg ctgatggcac tagattgtca
      841 actttctgca gaccaaaacc tatccgccat aaatcacgct tcgcttcata agacaagttt
      901 ggattgtcaa tgttgtaagt tggttgcata gaaatatcct ttcaattgtt tttcttcatt
      961 accagtaaat cacgaaattg gttggaaact ggcttaaact ataaaaagtc ctcacaatag
     1021 gttctatgat atatatatac ttcatgtaaa taataaaaga gttataatca gcactactga
     1081 taaattcaat accgatttcg ccatcttaaa acttagtcaa acgaataccg ttgatttcca
     1141 gttcattaag tcgttgacct gtcaagactt ctcgttcctt caactggaaa agttaagggg
     1201 gaactaatac aaatcctcta cgcttctata ttcaatg
//
LOCUS       DAVMGZ010000204         1171 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000204 DAVMGZ010000000
VERSION     DAVMGZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.205"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            18..1019
                     /locus_tag="VYQ93_001976"
     CDS             18..1019
                     /locus_tag="VYQ93_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3336605.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT          386 a          145 c          230 g          410 t
ORIGIN      
        1 cgtaatggag gactgaaatg aaaaatataa atgggaaaaa ggtaactatt tatgatattg
       61 cacgtttatc aggtttttct ccaaaaacag tttcacgagt tattaatggt ggagtaaatg
      121 ttaaagaaga aacttatcaa gcgattcaaa aagttataga ggaattgtcg tatattccaa
      181 atgcgtatgc aaaaaattta actaaaaaag aagctataaa tatattgatt tctgtaaaaa
      241 aaatcgactc ttttccttta atttggtttc atacgctgtt agataaagtg ttacggacgt
      301 gtaaagaatt tggtgttaat gctatagttg agtactttgg agaggaggat acaattagta
      361 attcaattat atcaagcaca ggtagcctag tagatggtgt tattgttttt tatgaaagtg
      421 tagatgatat cagaattcag tatttaaaga agaaccatat gccttttctt gttttcggtg
      481 aatctcaaac gtctggagta gtctatgtat ctaataataa ttttcaagct acttatgata
      541 tgatgaaagc tgtaactgaa gaaaaattta aaaatatgtg gttgcttatg gggggagaat
      601 cccatgttaa taaggatcgt gaaagaggtg tgcgttcttt tctgaatgat aaaaattact
      661 ttatggattt aaaagtcatt tatggcttat ctacaattga ttcagtttat tcttatgcaa
      721 tgcagcactt gactacccaa aattatccgg acattatttt tgtttcagga gatgaaaagg
      781 ttcagggctt gattcgtgcg tgctatgaga aaggaattct tattccagat gatatttcta
      841 ttattggatt tgataatatt cctatttctc agtattatac tccagctttg tctacaattg
      901 ctcccaatta tgttaaattg gctaaagaaa tgatagaagg tgttttagca attattaaag
      961 gggaaagtgt cacatctgtt gaagtttctc ctaaatttgt taggagacag agtttctagt
     1021 attgtttaat tcttgaaaat cctagtggaa aagatgaagt gttctaaaat aagttctgtt
     1081 tagcctccat tcagtttcta ttgagttgga tccaatttct aacaagtggg acatgttcat
     1141 gttcgatcta aggaagacat ggtcacattc g
//
LOCUS       DAVMGZ010000205         1159 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000205 DAVMGZ010000000
VERSION     DAVMGZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.206"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            53..268
                     /locus_tag="VYQ93_001977"
     CDS             53..268
                     /locus_tag="VYQ93_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV3336606.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(337..417)
                     /locus_tag="VYQ93_001978"
                     /pseudo
     CDS             complement(337..417)
                     /locus_tag="VYQ93_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(473..1009)
                     /locus_tag="VYQ93_001979"
     CDS             complement(473..1009)
                     /locus_tag="VYQ93_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV3336607.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
BASE COUNT          403 a          219 c          239 g          298 t
ORIGIN      
        1 aaatctacaa gattttacga ataaactaat gaaactaaaa aaggagaaga aaatggcaga
       61 atttacattt gaaatcgaag agcacttgtt gactctttct gaaaacgaaa aaggttggac
      121 caaggaaatt aaccgtgtga gctttaatgg tgcccctgca aagtttgata ttcgtgcttg
      181 gagtccagac catactaaaa tgggcaaagg gattactctc tcaaatgaag aatttcaaac
      241 gatggtggat gcctttaaag gcaactaata ctcttcgaaa atctcttcaa accgcatcag
      301 ctttgccttg ccgtatgtat ggttactgac ttcgtcagtt tcatccacaa cctcaaaaca
      361 gtgttttgag cagcctgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa
      421 gttttcaaaa taaaaaaaag gatattgagt catgataact cggtatcctt ttttagttag
      481 taaagtaagc ctgattttta aggcgttgaa gtaggggacg gctaataaaa ctaatagcca
      541 gaagtttgcc aaggtctgga atgataaagg gaagaacccc cacaacaaga gctttttcaa
      601 atgccattcc agctaggaaa tgcaagctga gaatcccgcc gacaaagaca agggcatcac
      661 ccaagaggtt tgcaagaaaa atcttaacaa caccactctt gctgttggtt agagaggaag
      721 taagtccaga gtaaacgaga taaaaccaaa gatagcctgc agtagggcca actaaagcct
      781 gaaaaccagc tccacctcct gcaaagacag gaagaccgat agcacctaga agaagataga
      841 gtccagcaga aagtacagcc tctctcggtc taaagacagt agcaatcaag ccgattgcaa
      901 agttttgcag agtgaaggga acaggtccaa ttggaagact gatttgtgcc aaaacagcaa
      961 tgagagcagc cccaatagca gggatagcat aaacgtgagc ttttttcaaa atagttaacc
     1021 tcttttctaa tgtatagtaa ctattatact catataggag tcgatgtcaa ccatttttta
     1081 aaaacaaggt aacaaaaact gtaacagccg attttcgtga ctagtcaagt tggataattt
     1141 ttttgttgat tcgaaaatt
//
LOCUS       DAVMGZ010000206         1154 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000206 DAVMGZ010000000
VERSION     DAVMGZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.207"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>1154
                     /locus_tag="VYQ93_001980"
     CDS             <1..>1154
                     /locus_tag="VYQ93_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV3336608.1"
                     /translation="YRHVTSLASLPSNAENYFMKVKSENFKDVMLPVTSITNDTKDNR
                     DVYKIVASANSLIQHENNNVLENYTYYLPKTQQSETGVYTSFKNLVDAMNSNPNGTFR
                     LGATMDAREVELPDGQESYVNNVFHGILVGTNNEKYYAIYNLKKPLFGELNGATVEKL
                     SLKDVNISAKDDTATLAKEANNNTHIDNVHADGAIAGERSIGGLVSQVNNSTISNSSY
                     TGRITNTYKTVASYQIGGLVGKLSGPRGLIDKSFASIDLSSNATQGDQSIGGIVGAVE
                     NSALISNSYAEGNLNNVQRFANVGGVVGNLWDPVGGLEKSGRLSNVLSDVNVTNGNAI
                     AGYNFNGIKANGTYSNKNNKVVNVVQEDDEILTKDSTVQRGEVLEDAQIK"
BASE COUNT          438 a          150 c          231 g          335 t
ORIGIN      
        1 gtatcgacat gtaacttcac ttgcttcttt accaagtaat gcagaaaatt actttatgaa
       61 agttaaatca gagaacttta aagatgtgat gctaccagtt acaagcatta ctaatgatac
      121 taaagataat agagatgttt ataagatagt agcaagtgca aatagtctga tacaacatga
      181 aaataataat gtactagaaa attatacata ctatttacca aaaactcagc aaagtgaaac
      241 tggggtatat acatcattta aaaatttagt agatgcaatg aatagcaatc ctaatggcac
      301 attccgttta ggagcaacta tggatgctcg tgaagttgaa cttccagatg gtcaagaaag
      361 ttatgtgaat aatgtattcc atggaatatt agtaggaaca aataatgaga aatattatgc
      421 tatttataat ttgaaaaaac cattatttgg ggaattaaat ggtgctacag tagaaaaact
      481 ttcactaaaa gatgttaata tttccgcaaa ggacgatact gcaacacttg cgaaagaagc
      541 aaataacaat actcatattg ataacgttca tgctgatgga gcgatagctg gggaacgtag
      601 cataggagga ttagtatcac aagttaataa ttctactatt tctaacagta gctatacagg
      661 tagaataact aatacatata aaacagttgc tagttatcaa atcggaggtt tagtaggcaa
      721 actttctggt ccgaggggat taatagataa gtcgttcgca tcaatcgatt tatcttcaaa
      781 tgcaacacaa ggagaccagt ctattggtgg aatagttggt gcagtagaaa atagtgcgct
      841 tattagtaat agttatgctg aaggaaatct aaataatgtt caacgttttg caaacgttgg
      901 tggagtagtt ggtaatcttt gggatccggt tggaggatta gaaaaatccg gtcgactttc
      961 taatgtacta agcgatgtta atgtaacgaa tggtaatgct attgcaggtt ataattttaa
     1021 tggtataaaa gcaaatggca catacagtaa taaaaataat aaggttgtga acgtagtaca
     1081 agaagatgat gaaatcttaa ctaaggattc tactgttcaa agaggagaag tattagaaga
     1141 tgcacaaata aaag
//
LOCUS       DAVMGZ010000207         1134 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000207 DAVMGZ010000000
VERSION     DAVMGZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.208"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..240)
                     /locus_tag="VYQ93_001981"
     CDS             complement(<1..240)
                     /locus_tag="VYQ93_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336609.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQV"
     gene            complement(254..967)
                     /locus_tag="VYQ93_001982"
     CDS             complement(254..967)
                     /locus_tag="VYQ93_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV3336610.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
BASE COUNT          367 a          224 c          191 g          352 t
ORIGIN      
        1 tacttgtgaa tcccttctcc caatataatc cccttctttg actaatgatg gagatttcca
       61 agtaaaaatt aaatcgccag agaaattttt cttatcaaaa aacttaaaaa gagattgata
      121 aatactattt gctaattgac aagtgtcaga tgtaaattca taattaacag cttcattaaa
      181 atcaacttca gacatttcaa tgtactcttc aatagtaata ttagcttttt ttgtagtcat
      241 tagctcatct cctttaaaat gtgatctttt caagaatatt tttatctaaa caatccagca
      301 agtcttggta agaatagact tcgtaagtcg gctgggcttg tgtgtgattt tcgaggtgat
      361 gaggattata ccagatagtg tcaatccccg cattattgcc accttgaatg tcggcggtta
      421 gagaatctcc aatcatcagc gtcttttctt tactaaatcc agcaatttgc tggccaatct
      481 tttcataaaa gagagcatcc ggcttttgag tttgcaactg ttctgagata aagacttgat
      541 tgaaataagg tactagacca gattgagcca aacgtcctgt ctgaatggca gtaatgccat
      601 ttgtcgcagc atacaagtca taatcacgct caatgaggct gtccaagaga tcatgagcgc
      661 ccgatagtgt ttgtccctgc tgagcgagat aaaattggta acgctgggca agaaaactac
      721 cgtctttttc ctgtccaaaa tgagaaaata aacgagaaaa gcgcgtgtta accaactctt
      781 gtttactgat tttcttcagc tccaagtctt tccagagagc cttgttcata ggaacgtaat
      841 aatctttata agcctgaata tccgcaactc cttcttcttt tagaagttgg gtcaaagcca
      901 catcctcagc agcatcaaaa tcaagaagag tgtggtcgag gtcgaagagt agaaatttgt
      961 agaacaattt gaggttttcc tttctgaaaa ttcattaaga acattatatc ataaagcacc
     1021 tcatacaatt aactaattta atcacttaaa aaaattcgaa cactttctat acaactgaca
     1081 gctcaaatct ttcagaatag aacaatacta actatcgaac accccgtctt cata
//
LOCUS       DAVMGZ010000208         1122 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000208 DAVMGZ010000000
VERSION     DAVMGZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.209"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            51..389
                     /locus_tag="VYQ93_001983"
     CDS             51..389
                     /locus_tag="VYQ93_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336611.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
     gene            455..>1122
                     /locus_tag="VYQ93_001984"
     CDS             455..>1122
                     /locus_tag="VYQ93_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3336612.1"
                     /translation="MKLKLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIQGKS
                     NLSNNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTL
                     GKMDVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRT
                     RQIKEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFF
                     SLLLT"
BASE COUNT          363 a          155 c          211 g          393 t
ORIGIN      
        1 gagactatta tttaatcttc atcaaatatt taaattcaag gagaagtaaa atgaaaaaaa
       61 gaattaataa gaaagcatct gtttttctag ttgcaagtgt tttcagtttt agctttgcta
      121 ctacagctag tgtcgttaac gcagtaacgg cacaccctgc tccaagtccc attattgatg
      181 tatgggaata tggtgtaaat gataactatg gttattcgaa ttactttgtt agttctccaa
      241 gtaatattgg atcaaaatct tcagtaacga atttttgggg aacagttaaa gctcaggata
      301 aaaaagatta tggatgggca tattcaagtg caacaaaatc ttggaacgat gttcgcttaa
      361 atgcatattg ggattattat agattttaaa aataatttat gaaaacaaga gacccaatta
      421 taagattggg tctcttgtaa tatggtaatt gcttatgaaa ttaaaattat gtatcattgg
      481 attcttcttt tgtttgattg ctacaattgg tctggtcact attagtgata ccgaaatccc
      541 cataccttta ccaattgatg gagctttttc tatccaagga aaaagtaatc tttctaataa
      601 cgaaatatac gaaatggttc gtgacttatc aaaaacggaa aaggttacca tttacaagcc
      661 aatcgttcag agttcgggtc agttgaagta tgtaaatttt gatgatgtaa ataatgaaca
      721 attaaagtca gcaccaatag tagggatgta ttacacactt gggaaaatgg atgtagacag
      781 tttgaaacca cttacgatga cgggactaca aacagtctat atggcttatc cttggtatat
      841 aggaggaata ttacaattca ctggaacttt aagaatactg ttaatgggtt caatttattt
      901 aactttatta gttgttttat ttgttgttag gacgaggcag atcaaagaag gagtgatacg
      961 acgttctttg ggcttgccta tatacgactt aagaagagag tatggcattt ctcttatttt
     1021 tgaactgatt atgatggctt tattgatgat ttcttacagt tcttttttgg gtaatggttt
     1081 cttcacctat agttctaagt tatttttctc tctgctttta ac
//
LOCUS       DAVMGZ010000209         1103 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000209 DAVMGZ010000000
VERSION     DAVMGZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.210"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..706
                     /locus_tag="VYQ93_001985"
     CDS             <1..706
                     /locus_tag="VYQ93_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336613.1"
                     /translation="YHQFLLGNNKTLQVLKNVSLSASRGEFISILGISGSGKSTLLKC
                     ISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIPSLPVIENIALPL
                     RLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAVIADSDIIFADEP
                     TGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILKDGKIFKELHRPS
                     GEELYKILEVQSTTEE"
     gene            709..>1103
                     /locus_tag="VYQ93_001986"
     CDS             709..>1103
                     /locus_tag="VYQ93_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336614.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYY"
BASE COUNT          358 a          147 c          223 g          375 t
ORIGIN      
        1 gtatcatcaa tttttactag gaaataataa aacgttgcaa gtgctgaaaa atgtttctct
       61 ttctgcttcg agaggagaat ttataagtat tctaggaatt agtggttctg gaaagtcaac
      121 tttattaaaa tgtatttcta gtttgcttga accaacaagt ggggaagtaa ttttaaatgg
      181 aatcaacccc tataaaatca gaaatgcaaa attgtcaagt ataagacgta acgaagtatc
      241 ttttatattt caagcataca atttaatacc ttccctgccg gtaatagaaa atatagcact
      301 tcctttgcga ttatcacaaa aaaaattaac tattaaaaat gtagaaaact tactcaaaag
      361 aatgaagttt aatgctggct taaacgattt tgttggaact ctgtctggtg gagaacaaca
      421 gaaggttgct atagctagag cggttattgc tgatagtgat ataatatttg ctgatgagcc
      481 aactggggct ttagacagcg tttctcgtga agtaattttt gaattattga gagagttagt
      541 aggggcgggt aagtgtgtaa ttatggtaac gcacgatata gaattggcct cgaaaactga
      601 tcgtgcatta atattgaaag atggaaaaat tttcaaagaa cttcatagac ctagcgggga
      661 agagttgtat aaaatcttag aggtacaatc aactacggag gaatagagat gctcagatta
      721 gtttactatc aatttttaca taataagaaa caatggttag gcgtttcacc agtaattttt
      781 gtttcgagtt tagttatggg attggctgtt aatggggtaa ttaatgttga gaataattcg
      841 caagtatttg tcggacttcc agatcctaaa ccaattttta tgtttccgat agtattcgga
      901 ggagtaacat tattctttgt gctctctaac ataataaata tgttggttga aatttttaga
      961 gatgattacg aacttctgga ggtgttggga gcaagtcgcc ttcaactatc atttcttgtg
     1021 gggggacaga tttttatcat ttcctcgatt attagtttta ttgcatatct atgctctata
     1081 tttgttacgt caaattatta tta
//
LOCUS       DAVMGZ010000210         1050 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000210 DAVMGZ010000000
VERSION     DAVMGZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.211"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..582
                     /locus_tag="VYQ93_001987"
     CDS             <1..582
                     /locus_tag="VYQ93_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV3336615.1"
                     /translation="FILNKDTGEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYK
                     GELEKGYQFDGWEISGFEGKKDAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSK
                     KKDNPQVNHSQLNESHRKEDLQREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKL
                     PKTGTASGAQTLLAAGIMFIVGIFLGLKKKNQD"
BASE COUNT          397 a          138 c          201 g          314 t
ORIGIN      
        1 ttcattttaa ataaagatac gggagaggta agtgaattaa aacctcatag ggtaactgtg
       61 accattcaaa atggaaaaga aatgagttca acgatagtgt cggaagaaga ttttatttta
      121 cctgtttata agggtgaatt agaaaaagga taccaatttg atggttggga aatttctggt
      181 ttcgaaggta aaaaagacgc tggctatgtt attaatctat caaaagatac ctttataaaa
      241 cctgtattca agaaaataga ggagaaaaag gaggaagaaa ataaacctac ttttgatgta
      301 tcgaaaaaga aagataaccc acaagtaaac catagtcaat taaatgaaag tcacagaaaa
      361 gaggatttac aaagagaaga tcattcacaa aaatctgatt caactaagga tgttacagct
      421 acagttcttg ataaaaacaa tatcagtagt aaatcaacta ctaacaatcc taataagttg
      481 ccaaaaactg gaacagcaag cggagcccag acactattag ctgccggaat aatgtttata
      541 gtaggaattt ttcttggatt gaagaaaaaa aatcaagatt aagacaaaag ctatagaaaa
      601 aaatggttta tgtactgaga ttagatagtg aggtgatgac atagttttgt gaaaacggcc
      661 atttataact caattattta gtttacttca cttcactatt gatactattt ggagttatta
      721 atggacttag tttagataac taatgaattg attgaaaggg ttagtattga caatattggt
      781 catattgatt agaaaataga gtctatcaaa atttaaaggc taatagaggt gatgagacaa
      841 tttcaaggaa ttaagtagcg tttggttgct taattccttt ttatttggaa ggaagttttt
      901 cgtaaccgcc caataacgaa gtctattgga cggttacgat atgatcactg acttcgtcag
      961 tcttatctac aacctcaaaa ctgtgttttg agcaacctgc ggctagcttc ctagtttact
     1021 ctttgatttt cattgagtat tagaacagaa
//
LOCUS       DAVMGZ010000211         1038 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000211 DAVMGZ010000000
VERSION     DAVMGZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.212"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            63..413
                     /locus_tag="VYQ93_001988"
     CDS             63..413
                     /locus_tag="VYQ93_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV3336616.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREDDTH"
     gene            628..744
                     /locus_tag="VYQ93_001989"
     CDS             628..744
                     /locus_tag="VYQ93_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV3336617.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            737..970
                     /locus_tag="VYQ93_001990"
     CDS             737..970
                     /locus_tag="VYQ93_001990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336618.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLASEVASQQLGMSVSEFEALL"
BASE COUNT          322 a          205 c          231 g          280 t
ORIGIN      
        1 gactttcctg atagagttat tcacatctta tttcaactca ctatagaagg aggaatagga
       61 ggattctcag acatccgggc atcagcccaa ctaatgattt gattgctaag aaaatattca
      121 gcaatccaga aatcacttgt caatttattc gcgatatgct ggacttgcca gcaaaaaatg
      181 tgaccatttt ggagggaagc gatattcacg tattactctc catgccttac tcggtgcagg
      241 atttttatac cagtatagac gtcttggcgg agttggataa cggtactcaa gtaattattg
      301 agattcaagt ccatcatcag aattttttca tcaatcactt gtgggcttac ctgtgcagtc
      361 aggttaatca aaatcttgaa aaaattcgtc agcgagaaga tgatactcac tagatctaca
      421 aacacatcgc tcctgtttac gtcattgcta tcgtggatag taattatttc tcagatgacc
      481 tggcttttca tagctttagt atgcgcgaag acacaacagg tgaggtattg gcgattacca
      541 acaatggaca ggaaaaccat ctggttaaga tggcattctt ggaattaaaa aatacagaga
      601 aaccagcaaa gacaaggttc gcaagccatg gttggagttt ttcggcaaca agccctttac
      661 ccagcaaccg caacgagcca ttacccaagc aaatcaactg ctggactaca agagctggtc
      721 cgaggaggac aggaaaatgt ttagtcaact acatatgcga gaagaacaag tcttgttagc
      781 acaggactat gccttggaaa ctgctagggc tgaaggcctt gaacaaggac tagagcgtgg
      841 gaaagttgaa ggaagggcag aaaggaaact ttttgccttc ctagacatag tacgccaagg
      901 tcttctggct tctgaggttg ccagccagca attaggtatg tcagtatctg aatttgaggc
      961 actgttgtaa aatggctcca taatatccat aggggattta cccactatgg atattatgga
     1021 gcctattttg tgtaaaaa
//
LOCUS       DAVMGZ010000212         1031 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000212 DAVMGZ010000000
VERSION     DAVMGZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.213"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..826)
                     /locus_tag="VYQ93_001991"
     CDS             complement(<1..826)
                     /locus_tag="VYQ93_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3336619.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEK"
BASE COUNT          326 a          144 c          181 g          380 t
ORIGIN      
        1 ctttttcgag tattttaact aatgcctctt ctttggttag tcgtaataaa gttattcctt
       61 tatcaatcaa atcggagtta tgttgtagta ttcccaagcg tacttgagag aatccaagaa
      121 tatcgtatat ttttaattct tctgctaata ccaaaagttg tagggtgatt gtttcacatt
      181 ctttgaataa atgatgttga aaatatacag tggagagatt ggctaaaaaa gatgataaaa
      241 atgcttttgt ttctcggaag tatttatatt tctcgatggt tttcataatt ctatcaataa
      301 gaaggggaag attttcagaa ggaaagaaaa agagaatggt tccaagcaat ttcaaatcac
      361 taatgtacca tgtatccata ggttcaagat agtcccaaat ttcttcatac aaatctttca
      421 agacgtggtt gtttttgaat cctttagttc gtaactctgt gacaattttt gtatgacgat
      481 agatattttg aataggaaca tcgtgatgtg tcttaagata tttttgacat ttctcagtga
      541 gttcaaccat ttttctagta tcttgaaaag tagacggatt ttggctctct acaataatat
      601 ctcgcctttt actaggatgg tattcgttgc atatatactt gaattctgcc aagctcatat
      661 caatttgttc aagaagaaat accatgtttt caaaactggg aataacttta ccgtgttcaa
      721 tatttgttag agttgttcga tgtatgactt gtccacatac atcttgttgg gtcaatcctt
      781 ttgactttcg aatttcttta aaaatttgtc cataatccca tctcatattg ttcctcctag
      841 gtaaatgtga atattttcta catttataaa aaacgtagaa aaaggtggta ttattatatc
      901 aaaaaggagg gaaaaagtca tgggctttaa aaaatatttg aagaatttac cgaaaaactc
      961 tggatttttg atttggagtt ggattcaact tatctggttt gaaacatggt tttggggata
     1021 aagaaattag a
//
LOCUS       DAVMGZ010000213         1021 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000213 DAVMGZ010000000
VERSION     DAVMGZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.214"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(58..672)
                     /locus_tag="VYQ93_001992"
     CDS             complement(58..672)
                     /locus_tag="VYQ93_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV3336620.1"
                     /translation="MPTALGYVSIGLVCGIIGVPYVTLVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVTAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFALILSSVVTGKVG
                     SLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAVLRLVF"
     gene            743..820
                     /locus_tag="VYQ93_001993"
                     /pseudo
     CDS             743..820
                     /locus_tag="VYQ93_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
BASE COUNT          330 a          230 c          215 g          246 t
ORIGIN      
        1 atcatttaca ccattatatg atatatatat ctgtgatagg ttttctttat ggctgattta
       61 aaagaccaaa cgcaagacgg caatcaagac tactccaaag agaactgttc cgactagatt
      121 gcggtagcga aaggctaccc aagctgttgg aaagacggct aagaagtcta gccatttaat
      181 ttgaggaagg ctcccaacct tacctgtcac tacgcttgaa agaatcaagg caaagatttc
      241 tggatttggt agcaggccac ccagagccgt tccgactact gtccccacaa accaagccac
      301 atagctgtta agattgtttc cgtgcatcca cataggattt accttgtctg tatgggctaa
      361 ttcgcccatc aaaacgccat aggtctcatc tgtcaagata ctagacatac cgatattgta
      421 ccaaagactg gtatgacgga aataagtcga tgcgtgtaaa ctcaacaaaa agagacgcaa
      481 gttgattaga aaaaccgtca tagcaatagc tgtcacagga gcttgaacca caatcagtgc
      541 caacatggca aactgggcac tcccagcata aacaaagaga ctcatcaagc ccatctcaac
      601 aagtgtcaca tagggcacac cgataattcc acagaccagg ccgatactga catagccaag
      661 agccgttggc atggctgcct gcgccccctc ctaaaatcct ttttctttca tctttctcct
      721 catattgtct taataatact caatgaaaat caaagagcaa actaggaaac tagccgcagg
      781 ttgctcaaaa cattgttttg aggttgtgga tagaactgac gaagtcagtt caaaacactg
      841 ttttgaggtt gtggatagaa ctgacgaagt cagctcaaaa caccgttttg aggttgtgga
      901 tagaactgac gaagtcagta accataccta cggcaaggtg aagctgacgt ggtttgaaga
      961 gagtttcgaa gagtacaagt aggctgaaaa gaatccagcc acagcatgga ctattatata
     1021 g
//
LOCUS       DAVMGZ010000214         1020 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000214 DAVMGZ010000000
VERSION     DAVMGZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.215"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            404..586
                     /locus_tag="VYQ93_001994"
                     /pseudo
     CDS             404..586
                     /locus_tag="VYQ93_001994"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          387 a          184 c          135 g          314 t
ORIGIN      
        1 actttatatg aaattctcat ttttgttttt acaattctcc ttagttaaat cttgtttaat
       61 atatgtttta catatagtat ttagcgccac atagtactga actctctcca aaaacggtta
      121 ttcctctttg aatagggcgt tatcacaaga aaagcatctc cacgtttcaa cttcatatgg
      181 ctcaaaaaca atcaattgat gctaaaacct gtacctagat gtttcggttc ataaaaccat
      241 gaaactgtaa aagtggatga aattgatagc gatagtcaaa tcaagaggca tcataactct
      301 aaaaagtcac aatatataag ttcatcctcg gaaaaatatc attctaattg ttgaaatgcc
      361 tacatgaaaa gaaacgtcaa atgctcatga aacaacgaat acaggtatca aaactatgac
      421 aaaacaagtc cctaaattta ctaaagacac tgctcaactt tacacctgta aatggttgtt
      481 gtataataaa gttacaaaga tgtacgacca cactgttgta aatcatagtg ttcgcgaata
      541 tattactgat agcatttcta caaatacaat tgaagagact ggatagactt taaacgaaat
      601 atgtcagtgt tttggcattc ctagccttca tatcatttaa agaattctat agacaaaatt
      661 tttcccaata cagacactcg taacaactgc ttcatttttc taccaacata tttaggaaca
      721 ggataagata caagaggatt aatccataga tcagttctat accaatctaa gacaaataag
      781 ctaaaaaaac gattgataat aagcaaatag attccaaatt ttctctatct gctcatttta
      841 ataaacaata ctagtgtaac tatccttcca gtcagaagct tgtcaaatca caccgaaaat
      901 tcttctaaaa tttatctcgt taggcaatca agcaaaaact cgacgatagt acaaacatta
      961 tcatacagaa ttgacttcct aaattatata ctttagtaag gttttcgtat aagaaaaaag
//
LOCUS       DAVMGZ010000215          981 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000215 DAVMGZ010000000
VERSION     DAVMGZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.216"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            143..>981
                     /locus_tag="VYQ93_001995"
     CDS             143..>981
                     /locus_tag="VYQ93_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV3336621.1"
                     /translation="MKVIDQALLEKVIIERSRTSHKGDYGRLLFLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMSFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDAFGEDLVKQVFAGLRQNQILIVDGGALTILARTSLSFSSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASALTSFPQGTILVEKGSATRIWQAGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFAGQFKQASLYERVAVATHLHSAIAQELAQEQYVVLPTEISNCLPKVMK
                     RYV"
BASE COUNT          294 a          178 c          238 g          271 t
ORIGIN      
        1 caatttgaaa atattattcc aactaaaata caagataggg tgcattatct tgactatgct
       61 ggagaagact tagaagagtg gactaagaaa gtctatcaag ttgttttaaa acaatcagat
      121 aaaggatagt tgaggaagaa cgatgaaagt gattgatcaa gccttactag aaaaagttat
      181 tattgaacgt tctcgtacta gtcataaagg agactatggc cgtctgctgt ttcttggtgg
      241 aacttatcct tatggtggtg ccatcatcat ggctgctttg gcagctgtaa aaagcggtgc
      301 aggattggtg accgttggaa cagataggga aaatattcca gccctgcaca gccatttacc
      361 tgaggctatg tccttttctc ttcaagacca gcagttgtta aaagagcaat tggagaaggc
      421 agaagttgtc ttgctggggc cgggtttacg agacgatgct tttggagaag atctagttaa
      481 acaggtcttt gctggcttaa gacaaaatca gattttgatt gtagacggag gtgccttgac
      541 cattcttgct aggacaagtt tgtcattttc ctcaagccag cttatcctaa ctcctcacca
      601 aaaagaatgg gaaaaattat ctggaattac gattgaaaag caaaaggaag atgcaacggc
      661 tagtgcccta acttccttcc ctcaaggaac aattttggtg gagaaaggtt cagctactcg
      721 tatttggcaa gctggccaat cagattatta ccagttacag gttggcggtc cctatcaggc
      781 gactggggga atgggagata cgcttgctgg gatgattgca ggatttgcag gccagtttaa
      841 gcaagctagt ctttacgaac gtgtggcagt agcgacccat cttcattcag ccatagccca
      901 agaactagct caagaacagt atgtggtttt gccaacggag attagtaatt gtcttcctaa
      961 agtaatgaaa agatatgtct a
//
LOCUS       DAVMGZ010000216          975 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000216 DAVMGZ010000000
VERSION     DAVMGZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.217"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..233
                     /locus_tag="VYQ93_001996"
     CDS             <1..233
                     /locus_tag="VYQ93_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV3336622.1"
                     /translation="ANRGGCSQSCRWKYDLYDMPFGKERKSLQGEIPEEFSMSAVDMS
                     MIDHIPDMIENGVDNLKIEGRMEWSLFTTYQQ"
     gene            254..724
                     /locus_tag="VYQ93_001997"
     CDS             254..724
                     /locus_tag="VYQ93_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV3336623.1"
                     /translation="MDAYLESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQ
                     LFGARRKIPEYKFVAEVISYDDATQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDL
                     HDAKGNKIDRAPNPMELLTIKVPQPVQAGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          312 a          166 c          214 g          283 t
ORIGIN      
        1 atgccaaccg tggtggatgt tctcagtcat gccgttggaa atacgacctt tacgatatgc
       61 catttgggaa agaacgtaag agtttgcagg gtgagattcc agaagaattt tcaatgtcag
      121 ccgttgacat gtctatgatt gaccacattc cagatatgat tgaaaatggt gtggacaatc
      181 taaaaatcga aggacgtatg gagtggagtc tattcactac gtatcaacag taaccaactg
      241 ctacaaggcg gctgtggatg cctatcttga aagtcctgaa aagtttgaag ctatcaaaca
      301 agacttggtg gacgagatgt ggaaggttgc ccaacgtgaa ctggctacag gattttacta
      361 tggtatacca tctgaaaatg agcagttgtt tggtgctcgt cgtaaaattc ctgagtacaa
      421 gtttgtcgct gaagtgattt cttatgatga tgcgacacaa acagcaacca ttcgtcaacg
      481 gaacgtcatt aacgaagggg accaagttga gttttatggt ccaggtttcc gtcattttga
      541 aacctatatt gaagatttgc atgatgccaa aggcaataaa atcgaccgcg ctccaaatcc
      601 aatggaacta ttgactatta aagtcccaca acctgttcaa gctggagaca tggttcgagc
      661 tcttaaagag gggcttatca atctttataa ggaagatgga accagcgtca cagttcgtgc
      721 ttaatgtagt tgtttagttt taaaaaatta tgcaaagctc catatacaac acttaaacga
      781 gattaaagaa tggcgaaatc ccttgatgcg caagagatta gctgtctttt ttatttttta
      841 agtgataaag tcggagctca ggtatcaaag cctatcaaat taaacaaaga agtgatgtct
      901 tagatatttt gaaaaaaatt aataagcaga aaactctcta ttattttgtt gtagagagtt
      961 ttttgttaat aaaat
//
LOCUS       DAVMGZ010000217          948 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000217 DAVMGZ010000000
VERSION     DAVMGZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.218"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..884)
                     /locus_tag="VYQ93_001998"
     CDS             complement(<1..884)
                     /locus_tag="VYQ93_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3336624.1"
                     /translation="MNLERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRN
                     RVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTL
                     NHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTSQNEAFLKSSETILNGFDVLA
                     SLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIK
                     GIVKIGTIEAIGALTGVIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEP
                     NEVNRDFPLYEAKNICYK"
BASE COUNT          333 a          170 c          128 g          317 t
ORIGIN      
        1 tacttatagc aaatattttt tgcttcatat aacggaaaat ctctattcac ctcatttggt
       61 tcgatatcat tcattttatt tgactcaatt ggattaattg aatacaattt taaaaaaata
      121 ggcttcgtac caataataga ggataattga cctcctaatt cacctagcgc tgtaaaaata
      181 acacctgtta gtgctcctat tgcttcaata gtaccaattt tcactattcc ttttattgca
      241 agatagcctg ttaaaaaaac gagagatatc tgaaaaaaaa tattgagaaa gaagctaata
      301 gcgcctgcta acgtttctac agttgtcttt ctttgtataa ccatctttaa taaaattcct
      361 gcttctttaa ttttcttagg caatacatat aaaagattca aggacgctaa cacatcaaat
      421 ccattcaata tagtctcact agattttaaa aaagcttcat tttggctagt taaatttaga
      481 ctaacttctc gcattttcga tgcaaagatt tttggtacaa gtagcataat cattaatgaa
      541 aacaaggtgg ctacagtcaa tgaccaatga tagtgattaa gagtcacaac tgcaaatata
      601 gtaccagaaa ttccttttat tactaaaaaa agttgtttaa aagcctgatc atttaaagtc
      661 tgaacatcat tatttagcca cgaaagatat gttcctgatg atttactatg aaattcttga
      721 taggtagagt tagagatgtc tgtggcaact ctatttcgaa tctctagatt aaactcttgg
      781 atcacttcaa cctgataatt tttcactacc cagtcaagga atattatccc acaccagaca
      841 atcatttggt agattgacaa tttcaaaaac cgctctaaat tcatcgcaat taattcattc
      901 aacaccagag cattaatagt tgctgcataa attagcaata attgacca
//
LOCUS       DAVMGZ010000218          884 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000218 DAVMGZ010000000
VERSION     DAVMGZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.220"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            20..817
                     /locus_tag="VYQ93_001999"
                     /pseudo
     CDS             20..817
                     /locus_tag="VYQ93_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          299 a          189 c          139 g          257 t
ORIGIN      
        1 ctagtataaa aaaattcaaa ctagaattct tcttagaaaa ggccgtttta agtgctatca
       61 ttgctcaaaa atggagctcg ctgagacttc tatcgtcaag aagaatcatc aaattccttg
      121 tatcatcaac caaaagattg ctcaaaagtt aattgaaaag atttctatga ccgacattga
      181 tcatcagttg tctatttcaa cttcaactgt cattcgcaag atcaataatt ttcactttga
      241 gcatgatttt tcgcgtcttc ctgagattat gtcctaggac gttgaaacag tccggggagt
      301 gactgtttca atcgggagat ggagatgagc tttattgctc aggactttga taaactcaat
      361 atcataactg ttcttgagag cagaacacaa gccatcatcc gaaatcccat gaatacaagg
      421 ctatcaagcg ctactggaag ctcattcaac aagatagtca gaaactgagt gataaacgat
      481 tttatcgccc tacttttcgt atgcacttga ctaataaaga aattctagat aaacttttga
      541 gctattccga agacttgaaa caccactata atctctatca gctcttgctt ttccacctct
      601 gtttcagact ttgtttaaaa ccttcctcaa agataaagag aaaatcgtca acgcccttca
      661 attaccttat tccaacgcca aactggaagc caccaataat ctcatcaaac ttatcaagca
      721 caatgccttt ggttttagga actttgaaaa cttcaaaaaa gaaaggacga aatttgtcct
      781 ttctagatct tcgctttctt caacccacta cagttgacaa agaaccgaaa aatgtctcgc
      841 ctctaatata gtcaattgaa acaagaacaa gagaaaagag cctc
//
LOCUS       DAVMGZ010000219          881 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000219 DAVMGZ010000000
VERSION     DAVMGZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.221"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(8..81)
                     /locus_tag="VYQ93_002000"
     tRNA            complement(8..81)
                     /locus_tag="VYQ93_002000"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Ile,seq:cat)
     gene            complement(95..168)
                     /locus_tag="VYQ93_002001"
     tRNA            complement(95..168)
                     /locus_tag="VYQ93_002001"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Met,seq:cat)
     gene            complement(174..247)
                     /locus_tag="VYQ93_002002"
     tRNA            complement(174..247)
                     /locus_tag="VYQ93_002002"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="VYQ93_002003"
     tRNA            complement(263..336)
                     /locus_tag="VYQ93_002003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(346..431)
                     /locus_tag="VYQ93_002004"
     tRNA            complement(346..431)
                     /locus_tag="VYQ93_002004"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Leu,seq:taa)
     gene            complement(439..510)
                     /locus_tag="VYQ93_002005"
     tRNA            complement(439..510)
                     /locus_tag="VYQ93_002005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Gly,seq:gcc)
     gene            complement(533..605)
                     /locus_tag="VYQ93_002006"
     tRNA            complement(533..605)
                     /locus_tag="VYQ93_002006"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Thr,seq:tgt)
     gene            complement(624..705)
                     /locus_tag="VYQ93_002007"
     tRNA            complement(624..705)
                     /locus_tag="VYQ93_002007"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(669..671),aa:Leu,seq:tag)
     gene            complement(711..783)
                     /locus_tag="VYQ93_002008"
     tRNA            complement(711..783)
                     /locus_tag="VYQ93_002008"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Lys,seq:ttt)
     gene            complement(808..880)
                     /locus_tag="VYQ93_002009"
     tRNA            complement(808..880)
                     /locus_tag="VYQ93_002009"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(845..847),aa:Asp,seq:gtc)
BASE COUNT          232 a          257 c          199 g          193 t
ORIGIN      
        1 attcaaatgg accttgtagg acttgaacct acgaccactc ggttatgagc cgagagctct
       61 aaccagctga gctaaaggtc cgacaagatc attatagcgg cgaaggggat cgaacccccg
      121 acctcccggg tatgaaccgg acgctctagc cagctgagct acaccgccat gaatcgggaa
      181 gacaggattc gaacctgcga caccttggtc ccaaaccaag tactctacca agctgagcta
      241 cttcccgagt taaatagaaa aatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctcca tattatgccg aggaccggaa
      361 tcgaaccggt acgatcgtta ccaatcgcag gattttaagt cctgtgcgtc tgccagttcc
      421 gccaccccgg cctctctaag cgaacgacgg gattcgaacc cgcgaccccc accttggcaa
      481 ggtggtgttc taccactgaa ctacgttcgc actgttttct tctatctaaa aatgccggct
      541 acatgacttg aacacgcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      601 ccggctcatt tgttatatct taatgcgggt taagggactt gaacccccac gccgttaagc
      661 gccagatcct aaatctggtg cgtctgccaa ttccgccaaa cccgcatata tgacccgtac
      721 tgggctcgaa ccagtgaccc attgattaaa agtcaattgc tctaccaact gagctaacga
      781 gtctaaaata acttgcgtta ccttaaacgg tcccgacggg aatcgaaccc gcgatcttcg
      841 ccgtgacagg gcgacgtgat aaccgctaca ctacgggacc t
//
LOCUS       DAVMGZ010000220          874 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000220 DAVMGZ010000000
VERSION     DAVMGZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.222"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..178)
                     /locus_tag="VYQ93_002010"
     CDS             complement(<1..178)
                     /locus_tag="VYQ93_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV3336625.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDT"
     gene            complement(191..>874)
                     /locus_tag="VYQ93_002011"
     CDS             complement(191..>874)
                     /locus_tag="VYQ93_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV3336626.1"
                     /translation="LSEKLNVSKVTIRKDLDKLESKGLLHREHGYAVLNSGDDLNVRL
                     SINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAEEICKQKRNVTIVTNSFFIANF
                     VRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHVRQAFVGTDGYDKEMGFTGKDL
                     MRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALSQVYEVITDEKLSKQNMATLEN
                     AGIMVKVVS"
BASE COUNT          265 a          184 c           96 g          329 t
ORIGIN      
        1 tggtatcaaa attctctatt tcaattttaa caattcctgc attccttgct tctgttaaca
       61 ttctactaat agaggttcta taaattccta attttgctgc tatttgtgac tgatttaagt
      121 tttcaatata atacagataa gcaattttag agagcagttt attcctatct tgattcatac
      181 acttaacctc ttacgaaact accttaacca ttatcccagc attttctaat gtagccatat
      241 tttgtttaga aagtttttcg tctgttatta cttcatagac ttgacttaaa gcaaatcttc
      301 ttactgtacc ccttttatca aatttacttg agtcagttag cacaatgact ttatccgacg
      361 ctgctgaaat atattgaact acctcactgc gcattaaatc ttttcctgta aagcccatct
      421 ctttatcgta gccatctgtc ccaacaaaag cttgacgcac atgaaaagtc tgtatcattt
      481 cttttaataa aggtcccaca gtaacctgtg aatccttctg aaactctcca ccaagaacaa
      541 taatacgaca tgaatcataa gctctcacaa aatttgctat aaaaaatgaa tttgttacaa
      601 tcgtaacatt tcttttttgc ttgcaaattt cctcagcaag taaagcacag gtcgatccag
      661 attctatcat tattgtttca ttatctgaca ccaattttac tgcttcctga acaatttttc
      721 tcttaatttc atagttaatt gacaaacgta catttaagtc atctccacta tttaatacag
      781 catatccatg ctctctgtgt aataaacctt ttgactctaa tttatctaaa tcttttctaa
      841 tcgttacttt cgatacattt aatttttccg ataa
//
LOCUS       DAVMGZ010000221          871 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000221 DAVMGZ010000000
VERSION     DAVMGZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.223"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..673)
                     /locus_tag="VYQ93_002012"
     CDS             complement(<1..673)
                     /locus_tag="VYQ93_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336627.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFT"
     gene            complement(683..>871)
                     /locus_tag="VYQ93_002013"
     CDS             complement(683..>871)
                     /locus_tag="VYQ93_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336628.1"
                     /translation="FTFLFVESLFWIYQLDNGIIGLLPIFQYMVNSNPYALIYWLTLL
                     SIIIPLTVFSVHRNWRRV"
BASE COUNT          372 a          169 c          101 g          229 t
ORIGIN      
        1 gagtgaaata gaaaatactt ttaaaaagaa agactaaaaa tagcaactga agaaataaat
       61 ctcgtcctat atctccattc cctcgattca aaatataaaa tagataatta gttcgatttc
      121 ctacaaatag accaccaaac acaatcatga gtaaaaagaa actaacgcaa gcaaagttca
      181 tttgtacatt cgctcctagt gcgtataaca taaagcaaaa caagactaac acacaagcat
      241 tcaaagaatc aactataaac aaaactccta tatcccagtt tggtaactgt aacataaatt
      301 gaatcactag aacttttacc atgattaaaa tgagtccata aagaaaacag gtccaaatct
      361 gatacctaat ccagagctta atagaagaaa atcgaatcac ttccataaag aaatcggatg
      421 ctcttctacg atatacaact gctagaaaaa atgtatgaat aataaaccaa tgaagcaacc
      481 atatagataa gactaaaaaa gagttttgac tcagattagg cccttttaaa atttctataa
      541 caagtctatc atttccttct aaccaatacc ataatcctaa acaaatcaat atccaaaaaa
      601 gaacataacc ccaaaagtac ttggtataga ttatctgatt caacctccac atgtgactac
      661 ttaactttcc catttccaac ttttacactc tcctccagtt tctatgaaca gaaaatacag
      721 tcaatggaat tatgatagat agtaatgtaa gccaataaat caatgcatac ggattggaat
      781 ttaccatata ctgaaaaatt ggcaataatc caattatccc attgtccaac tgataaatcc
      841 agaataagga ttctacaaat aaaaaagtaa a
//
LOCUS       DAVMGZ010000222          860 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000222 DAVMGZ010000000
VERSION     DAVMGZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.224"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(289..459)
                     /locus_tag="VYQ93_002014"
     CDS             complement(289..459)
                     /locus_tag="VYQ93_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336629.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(472..>860)
                     /locus_tag="VYQ93_002015"
     CDS             complement(472..>860)
                     /locus_tag="VYQ93_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3336630.1"
                     /translation="EEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRIALARA
                     LINNPSIVILDEATSALDTINEERITKYIQSQGCTQIIVAHRLSTIKDADVIFVMKGG
                     KIVESGNHKYLMDLGGEYYSLYTKRK"
BASE COUNT          277 a          175 c          109 g          299 t
ORIGIN      
        1 ttatactcaa tgaaaatcaa agagcaaact aggaagcgag ccgcaggctg tacttgatta
       61 cggcaaggca aagctgacgt ggtttgaaga gattttcaaa gagtattaaa caaaaagaaa
      121 aaccgctact cctaagaata gcgatttaat catgttttta agctactact gtagttgctc
      181 tattatttaa gtgttatgaa taacttcaat atctttaaag ctttttgtta gatttataat
      241 ttcaactata gccatctcta ctccttagtt tacagcaaaa aattatgctt aattcttatt
      301 gtttttgtaa acaatcagcg aacccaatac tattccattc aacaagccta aagaaatacc
      361 atattcaact ggaaaatcta aaaaaattgc aaacactatt ccaatcatca cccctagtga
      421 ggctgttgtt aaaattacat attcattttc tttcttcatt ttctttacac ctcatttcct
      481 ttttgtatat aagctgtagt actctccacc aagatccatt aagtacttat gatttcctga
      541 ctcaacaatc ttaccacctt tcattacaaa aataacatcc gcatccttaa tcgttgacaa
      601 tctatgagct acaattattt gagtacagcc ctgactttgt atatactttg ttattctttc
      661 ctcattaata gtgtctaatg cactagttgc ttcatctaaa attacaatac taggattatt
      721 tattaatgca cgtgccagtg ctatccgttg cctttgccca cctgaaatat ttgaccccat
      781 ctctgagatg atagtattaa atttcatcgg catagccatg atttcatcat agatttgaac
      841 tgctttacaa acttcctcta
//
LOCUS       DAVMGZ010000223          851 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000223 DAVMGZ010000000
VERSION     DAVMGZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.225"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            214..594
                     /locus_tag="VYQ93_002016"
     CDS             214..594
                     /locus_tag="VYQ93_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV3336631.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQIQLAELEINK"
BASE COUNT          278 a          115 c          171 g          287 t
ORIGIN      
        1 acagatagtg taaataaaga taaactattt actggctaat taatcagtta aacactagtt
       61 aaggagtaat gatgaaaaaa agaacaatac tattattgat ggccagtctg ttagctcttg
      121 tcttaggagc atgtggtttc ttggacatat tgatcctgga tcattctcat caggattact
      181 ctttactgct attttagaaa ctggagtggt ttgatggaaa gtattggtat tggtcttgtt
      241 atcgtttcac attccaaaca tattgcagaa ggtgttgttg aactgattag taaagtagct
      301 aaagatgttc cgattactta tgtaggagga accgagggcg gaggaattgg aacgagtttt
      361 gatcaagtag atagggttgt ttccgaaaat ccagcagata ctttacttgc cttttttgac
      421 ctaggttctg ctaaaatgaa cttaaaaatg gtgactgatt tcagtgataa aagtatcatc
      481 atcaacaggg ttccaattgt agaaggtgcc tataatgcag ctgctcttct tcaggctggt
      541 gcagaactgt cagttattca aatacagtta gcggagcttg aaatcaataa ataaggaatt
      601 ttactataac tctttttata gataagctat tgattatctc aactataaaa tgttaagtaa
      661 atgatttcaa aatcagaaag ggattgcttt atgcagttcc tttttatttt aacaggagtg
      721 aaactatagt gtttctaaat tgtgaatcaa tcaaaactga ttgtgatggg gctattctag
      781 ctttagaatc cttcaaaaat taaaatttaa ggcaatcaat tacttggaag agtatgaaag
      841 catttagttt a
//
LOCUS       DAVMGZ010000224          845 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000224 DAVMGZ010000000
VERSION     DAVMGZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.226"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..845
                     /locus_tag="VYQ93_002017"
                     /pseudo
     CDS             1..845
                     /locus_tag="VYQ93_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238800.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          254 a          179 c          153 g          259 t
ORIGIN      
        1 agtgctatca ctgttcaaaa atgatggttg ctgagactcc cctggtaaag aaaaatcacc
       61 aaatccctcg tatcatcaac caaaaaattg ctcaaaagtt aattgaaaaa atttctatga
      121 ctgatattgc ccatcagctg gccatttcaa cttcaactgt cattcgcaag ctcaatgact
      181 tccactttga gtgtaatttt agaaatctgc ctgagattat gtcttgggag gttgaaacag
      241 tccggggagt gactgtttca atcgggagat ggagatgagc ttcattgcgc aagattttga
      301 aaagctcgat attatcactg ttcttgaggg tagaacacaa gctatcattc gaaatcactt
      361 tcttcgctac gatagagttg tccgatgtcg cgtcaaaatt attactatgg atatgtttag
      421 tccttactat gacttggcta aacagcttcg ctttcaaatt tctaggctca ggctgaaaca
      481 gtctcccaga ctgtttcact cccgaatgct aaaatcgttc ttgatcgctt tcatattgta
      541 caacatctta gccgtgctat gagtcgtgtg cgtgtccaaa tcatgaatca gtttcatcga
      601 aaatcccatg aatacaaggc tatcaagcgc tactggaaac tcattcaaca ggatagccgt
      661 aaactcagcg ataaacattt ttatcgccct acttttcgta tgcatttaac caataaagag
      721 attttagaca agcttttgag ctattcacaa gacttgaaac atcactatca gctctatcaa
      781 ctcttgctgt ttcactttca gaataaggaa ccagagaaat ttttcggact tattgaggac
      841 aatct
//
LOCUS       DAVMGZ010000225          833 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000225 DAVMGZ010000000
VERSION     DAVMGZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.227"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..441
                     /locus_tag="VYQ93_002018"
     CDS             <1..441
                     /locus_tag="VYQ93_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3336632.1"
                     /translation="QQDSRKLSDKHFYRPTFRMHLTNKEILDKLLSYSQDLKHHYQLY
                     QLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLE
                     ATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(545..632)
                     /locus_tag="VYQ93_002019"
     tRNA            complement(545..632)
                     /locus_tag="VYQ93_002019"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(596..598),aa:Ser,seq:gct)
     gene            complement(639..712)
                     /locus_tag="VYQ93_002020"
     tRNA            complement(639..712)
                     /locus_tag="VYQ93_002020"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(676..678),aa:Ile,seq:gat)
     gene            complement(748..818)
                     /locus_tag="VYQ93_002021"
     tRNA            complement(748..818)
                     /locus_tag="VYQ93_002021"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(784..786),aa:Gly,seq:tcc)
BASE COUNT          275 a          195 c          137 g          226 t
ORIGIN      
        1 caacaggata gtcgtaaact cagcgataaa catttttatc gccctacttt tcgtatgcat
       61 ttaaccaata aagagatttt agacaagctt ttgagctatt cacaagactt gaaacaccac
      121 tatcagctct atcaactctt gctgtttcac tttcagaata aggaaccaga gaaatttttc
      181 ggacttattg aggacaatct taagcaggtt catcctattt ttcagactgt ctttaaaacc
      241 ttcctcaaag ataaagaaaa gattgtcaac gcccttcaac tacactattc taatgccaaa
      301 ctggaagcga ccaataatct catcaaactt atcaagcgca atgcctttgg ttttcggaac
      361 tttgaaaact tcaaaaaacg gattttcatc gctctgaaca tcaaaaaaga aaggacgaaa
      421 tttgtccttt ctcgagctta gcttttcttc aacccactac agttgacaaa gagccttaag
      481 tttacggatc tgaagtttta ttgtctctaa aaacaaaaaa gagagttaca aactctcatt
      541 aaaacggaga ataagggatt cgaacccttg cgccagttac ccgacctaac gatttagcaa
      601 accgtcctct tcagcctctt gagtaattct ccaattaatg ggcacgagtg gactcgaacc
      661 accgacctca cgcttatcag gcgtgcgctc taaccacctg agctacgcgc ccaagttaaa
      721 aaacttggta atttgaacaa agttcaaagc gggtgacgag aatcgaactc gcgacaacag
      781 cttggaaggc tgtagtttta ccactaaact acacccgcat aaatactatc aat
//
LOCUS       DAVMGZ010000226          823 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000226 DAVMGZ010000000
VERSION     DAVMGZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.228"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(3..140)
                     /locus_tag="VYQ93_002022"
     CDS             complement(3..140)
                     /locus_tag="VYQ93_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV3336633.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            complement(342..>823)
                     /locus_tag="VYQ93_002023"
     CDS             complement(342..>823)
                     /locus_tag="VYQ93_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3336634.1"
                     /translation="LYGSSLISFLWMYGQRKQAHKVNMKSRMKWLGIGFVSLLIISLC
                     FSLIHAQESTNQANLIGLQHQVPWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVA
                     LTSVLFALAHHPGTIIAWCLYVSLGMFLGLVRYKLDLWGSMGLHLVWSLLVYSLLLF"
BASE COUNT          288 a          179 c          113 g          243 t
ORIGIN      
        1 aattaacaat tttcccaact aaaagcactc cagttaccgc aacggtttgt actgaatgta
       61 ctaaatcgca ttccatcaac ttcatctgtt tcgtcaactt gaacagatac taattgaaga
      121 tttaatactt cttctgccat agctaactcc tcctatttaa atttttggga ttaagtgctt
      181 tatccacctt cattatactc tctccagcag taaaatgcaa gcaatgatac aatgttgtca
      241 cgtagaaaat aatgtttccg taacttttca aagtaacttc catctctctc ccaaaactgg
      301 aagttagttt tagaagttac ctaaaaatca ggttacctat tttaaaaaag cagcaaacta
      361 taaactagta ggctccacac caaatgtagc cccatactgc cccataagtc caatttgtag
      421 cgtacaagcc ctaaaaacat ccccagcgat acatacaaac accaagctat aatggttcct
      481 ggatgatgtg ctaaggcaaa taaaacactt gtcaaagcaa ctcgaatatc taattttcta
      541 accaagttcc ataaaatttc tctatacaga aattcttcaa ccatactcgc attgattaaa
      601 aacaataaaa atgaaaacca aggaacttga tgttgaagtc caattaagtt tgcttgattc
      661 gtgctttctt gcgcatggat taggctaaaa catagactta taatcagtag gctaacgaat
      721 ccaataccaa gccatttcat cctagatttc atatttacct tatgagcttg tttgcgttgg
      781 ccatacatcc ataaaaaaga aatgagtgac gaaccataga gaa
//
LOCUS       DAVMGZ010000227          814 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000227 DAVMGZ010000000
VERSION     DAVMGZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.229"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..718
                     /locus_tag="VYQ93_002024"
     CDS             <1..718
                     /locus_tag="VYQ93_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEV3336635.1"
                     /translation="TILSERISQVAAGVLRNLIDDNMKIGFSWGKSLSNLVDLIHSKS
                     VRNVHFYPLAGGPSHIHAKYHVNTLIYEMSRKFHGECTFMNATIVQENKLLADGILQS
                     RYFENLKNSWKDLDIAVVGIGDFSNKGKHQWLDMLTEDDFKELTKVKTVGEICCRFFD
                     SKGKEVYENLQERTIAISLEDLKNIPQSLAVAYGDTKVSSILSVLRANLVNHLITDKN
                     TILKVLEEDGDLTFREILGE"
BASE COUNT          280 a           92 c          161 g          281 t
ORIGIN      
        1 aacaatttta tctgaaagaa tttctcaagt tgcagcaggt gtccttagga atctaattga
       61 tgataatatg aaaattggct tttcttgggg gaaaagttta agtaatttag tagatttaat
      121 tcacagtaaa agtgtccgaa atgttcactt ctatcctcta gcaggtggtc ctagtcacat
      181 acacgctaaa taccatgtga atacactgat ttatgaaatg tctagaaaat ttcatggaga
      241 gtgtacattt atgaatgcaa cgattgtgca agaaaataaa ttgttagcag atggtatttt
      301 gcaatcaaga tattttgaaa atttgaaaaa tagttggaaa gatttagata tagctgtagt
      361 cggaattggt gattttagca ataaaggaaa gcatcaatgg ttagacatgc ttacagagga
      421 tgactttaaa gaattgacca aagttaaaac tgtaggagaa atttgttgtc gattttttga
      481 ttcaaaaggt aaagaagtct atgaaaactt acaggaaaga acgatagcaa tctctttgga
      541 agatttaaaa aatattcctc aaagtttagc tgttgcttac ggtgatacga aagtatcttc
      601 gattctttct gtcttgcgtg ctaatttagt aaatcatttg attacagaca aaaatacaat
      661 tttaaaagtt ttggaagaag atggggattt gacttttaga gagattctag gtgagtgaaa
      721 atgatagact gattcagttt atcgtttttc tttttagttg attgcacatt tgtgcttata
      781 taaacaaaaa tagtttatct gttgtttttg gatt
//
LOCUS       DAVMGZ010000228          804 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000228 DAVMGZ010000000
VERSION     DAVMGZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.230"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..624)
                     /locus_tag="VYQ93_002025"
     CDS             complement(<1..624)
                     /locus_tag="VYQ93_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV3336636.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLA"
BASE COUNT          238 a          147 c          134 g          285 t
ORIGIN      
        1 tgcgagtaaa tattttttat tagaattatt taaagcgatg cgttgagcct ctccctcttc
       61 ctcgctaaaa gtagctagag ctccgttttg aataccatta cagctaacga aagctttaga
      121 aaattggaga ttagagagat tttgtagggt caatgtgcca acaaaagcac ctgtaatatc
      181 gcgataattt ccacctatca aaatcaaatc tgttaatttt cgttcgctta aaatcagaaa
      241 aacaggtaga ctgttggtta cgacgcggat attgtcaata ggcaactcac gcgcaaaaaa
      301 ctctaatgtt gttcctggtc caatgaaaat agtttctctt tcttctacta gactgcctgc
      361 aaaatgggct atttcttgtt tttctgccgt ttggagggct tgtttttcaa tatttgatcg
      421 ctcattagtc aaaagggagt tggttcgaag tttttcagct ccaccatgca cacgaatcag
      481 caaatcttta tcagctaatt cctgtaaata gcgccttgca gtcatatctg aaacggctat
      541 ttcgtccata atctgtttaa ctgttatggt tcctttacta tttactatct ctaaaatttt
      601 ggttaatttt tcttgtttga gcatattatc acctcgtttc ctactactat cttaccataa
      661 acaaactcat cattcaaata caaaaacaac aaaatgaaac aaaaacaaaa atatcgaagt
      721 ttgttttcaa aactttcgat atttttgttg ggttattatt ttgttgtttc tagtttactt
      781 tttgatggtt aagagtggtg gaga
//
LOCUS       DAVMGZ010000229          787 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000229 DAVMGZ010000000
VERSION     DAVMGZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.231"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            95..232
                     /locus_tag="VYQ93_002026"
     CDS             95..232
                     /locus_tag="VYQ93_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV3336637.1"
                     /translation="MDFEYFYNREAKRFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     K"
     gene            239..673
                     /locus_tag="VYQ93_002027"
     CDS             239..673
                     /locus_tag="VYQ93_002027"
                     /inference="COORDINATES: protein motif:HMM:NF018653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV3336638.1"
                     /translation="MSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDIK
                     KGIGLIERVRLGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQ
                     EVKNLDSNYIENNKSKYSKREYSFGENGLGTFQNVFLAAEDI"
BASE COUNT          316 a          101 c          143 g          227 t
ORIGIN      
        1 tttaaaaaat ttcataaaaa tggttaccaa aggcgataga agaaaaacta tcgtcttttt
       61 ctttgcaaat ttttaagaag ggaggtgatc ttgcatggac tttgaatatt tttataacag
      121 agaagcgaaa agatttaact tcttaaaagt accggagata ttagttgata gagaagaatt
      181 tcggggctta tcagcagaag caattatcct ttattccata cttcttaaat agacaggaat
      241 gtcatttaag aataactgga tagacaagga aggcagagta tttatctatt ttactgtcga
      301 agaaattatg aaaagaagaa atatctcaaa gccaactgcc ataaaaacat tagatgagct
      361 tgatataaaa aaaggaatag gactgatcga aagagtaagg cttggacttg gtaagccgaa
      421 catcatttat gttaaagact ttatgagtat atttcaggta aaagaaaatg acttacagaa
      481 gtcaaaaaac ttaacttcag aagtaaaaga ttttaacctc agaagtaaag aaaatgaact
      541 tcaagaggtt aagaaccttg actctaacta tatagagaat aataagagta agtatagtaa
      601 gagagaatat agttttggtg aaaacggact tggaacattt caaaatgtgt ttttagctgc
      661 tgaagatata taggatttac aaatcataat gaactcacag cttgagaatt acattagact
      721 tcctgcaaaa ctagaatcct agttcatgat tgataatacc agcaatcaaa ttcattcgta
      781 atccgaa
//
LOCUS       DAVMGZ010000230          779 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000230 DAVMGZ010000000
VERSION     DAVMGZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.232"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..352
                     /locus_tag="VYQ93_002028"
     CDS             <1..352
                     /locus_tag="VYQ93_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3336639.1"
                     /translation="KTVYDNLSLITKDKKTITDVLEKVGLSSDYLHQKIYELSGGQAQ
                     RVAIARMLMKPRKIILADEPTGALDGEIGKEIIRLLLNETAEDKYVIIATHDPAVYNE
                     VDVIIDMKDIGYKV"
     gene            349..>779
                     /locus_tag="VYQ93_002029"
     CDS             349..>779
                     /locus_tag="VYQ93_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336640.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFDYGKEEL"
BASE COUNT          298 a          104 c          160 g          217 t
ORIGIN      
        1 caaaacagtg tatgataatc tgagccttat cactaaagac aaaaaaacaa taactgacgt
       61 acttgaaaaa gtaggactgt caagtgacta tttacaccaa aaaatatatg aattgtctgg
      121 agggcaggct caacgtgtag ctattgccag aatgttaatg aaaccacgta aaattatctt
      181 agcagatgaa cctacaggag ctttagatgg tgagattgga aaagaaatta ttcgtttact
      241 attgaatgaa acagctgaag ataaatatgt cattatagcg acacatgatc cagctgtcta
      301 taacgaagtt gatgtgataa ttgacatgaa agatataggg tataaggtat gaaaaagaaa
      361 atttatattg ctttgatctt tgtaactggg gtactcgcca ttttcttttt tggaaaacaa
      421 atgattacaa aagaaaatat caataagcca acggtagagt taacaatcta tacgctttct
      481 tctagtgata cagaaaaatg gaataaagtg agacaagtag aaacggaaga agctatatac
      541 ttcataactg taaaagaagt atcatcttca gaagaagtat tttcaaacat cattgcaaat
      601 ggagctgcta cggggttcgg agttcgtgaa gaagaagtga agaaattcaa taatggttta
      661 ggagacacca tagaggactc taagcataat aaactaattg aaatagaatt ttttactttt
      721 tctgatgatg gcgcgggatt tgtggtagca aactttgact acggtaaaga agagttgaa
//
LOCUS       DAVMGZ010000231          767 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000231 DAVMGZ010000000
VERSION     DAVMGZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.233"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            62..385
                     /gene="tnpB"
                     /locus_tag="VYQ93_002030"
     CDS             62..385
                     /gene="tnpB"
                     /locus_tag="VYQ93_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV3336641.1"
                     /translation="MTIHLSSLGEVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLIWLSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
     gene            complement(540..767)
                     /locus_tag="VYQ93_002031"
                     /pseudo
     CDS             complement(540..767)
                     /locus_tag="VYQ93_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          215 a          147 c          143 g          262 t
ORIGIN      
        1 agtttttttc aatctctcaa tctcgaaatg gtagaacagc ttttggataa ggtgttgctc
       61 tatgacaatt catctatcta gcctagggga ggtctatctc gtgtgtggga aaactgatat
      121 gagacaagga atcgattcac tggcttatct ggttaaaacc cactttgaat tggatccttt
      181 ctccggtcaa gtctttctct tttgtggtgg acgtaaagac cgctttaaag tcctttactg
      241 ggatggtcaa ggattttggc tactatataa acgctttgag aacggcagat tgatttggct
      301 aagtacagaa aaggatgtca aagctctcac accagaacaa gtagactggc ttatgaaggg
      361 cttttctatc actccaaaaa tatagtagat tgaaactaga atagtacacc tctgcttcta
      421 aaacattgtt agaaatcgat tttactgtcc tgatcgattt gtcctgttct tatttcattt
      481 tactataaat ccatcagaaa gtcgtgattt ctattgaaat gaggactttc tttttatact
      541 catctgcttt caaaaagcat tctagtccat ctccgattaa cgatggactt tatcacctcc
      601 ttctccagtc cttgtataac atcttggagt tgattcatga catcttccaa agtttaaaag
      661 gctttattct taaatccacg tttacgaatc tctttccaca cttgttcaat ggggttcatc
      721 tctggtgtgt atggaggaat aaatgcaaag ccaatattag tcggaat
//
LOCUS       DAVMGZ010000232          743 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000232 DAVMGZ010000000
VERSION     DAVMGZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.234"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(114..362)
                     /gene="blpK"
                     /locus_tag="VYQ93_002032"
     CDS             complement(114..362)
                     /gene="blpK"
                     /locus_tag="VYQ93_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HEV3336642.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(431..700)
                     /gene="blpJ"
                     /locus_tag="VYQ93_002033"
     CDS             complement(431..700)
                     /gene="blpJ"
                     /locus_tag="VYQ93_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEV3336643.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
BASE COUNT          233 a          192 c           93 g          225 t
ORIGIN      
        1 tcaatagtct tttgtttttt atcagagata cttatggata gataaataag ataggtttga
       61 aaagcgaaga gaataataaa gaatatagcc ttcataaaat ttagctttca tttttatgat
      121 gtagcggtat aggctaaata tccacaaacc actgctcctc caattcctcc tattgcagcg
      181 ccccatggtc ctagaatttt cccatatttc actccacccg ctgcacaacc taaagcagca
      241 actacagctg ctcctccaac acctgctttg gcaaaatctc cccaattgca tccgccacct
      301 tcaacgcaag caagcatttc agtatccata acagaaaatt gtgacatcat ttttgtatcc
      361 atgacaaata ctcctttttt aaaaaactaa aataaatcag aatagaatcc tcataatttt
      421 actataagtc ttaccaactt agtcccaatt tatcaccaac catacctcct aagcatgtta
      481 atccaccccc aattgcacca atgtgtgctc caacaaatgc accagcaagt ccagctactc
      541 ctaaagtggc caaacctgct ccagttccac cagttataat tcccgtagtg actcctgtaa
      601 tcagtgcatt ttgacaatca gtggagctat accccccttc aactttcgca agcatttcag
      661 tatccataac ctctaactgt gacaacattt ttgtattcat gatgaatacc tcctttttat
      721 tttcaatttg ttaccaaagt ctt
//
LOCUS       DAVMGZ010000233          706 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000233 DAVMGZ010000000
VERSION     DAVMGZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.235"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..706
                     /locus_tag="VYQ93_002034"
                     /pseudo
     CDS             1..706
                     /locus_tag="VYQ93_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          274 a          120 c          146 g          166 t
ORIGIN      
        1 gtcgtacgat acttcttaac tggctagcac aatacaggaa aaacgggtat actattgttg
       61 agaaaacaag agggagagta cctaaaatgg gacgtaagcc aagaacgaga cctgaagaga
      121 ggacagaatt agaacgtctt caagcagaaa atgagtacct gagagcggag aatgccatcc
      181 taaaaaagtt aagagaactc cgattgaagg aggaaaaaga gaaagaagaa agacagaaat
      241 tgttcaagaa ttaatgactg agttttcgtt agatattctt ctaaaagcca ttaaactagc
      301 tcgttggacc tactactatc acttgaaaca gctagacaaa acagataagg accaagagct
      361 taaagctgaa attcaatcca tttttatcga acacaaggga aattatactt atcgccgggt
      421 tcatttagaa ctaagaaatc gtggttatct ggtaaaccat aaaagagttc aaggcttgat
      481 gaaagtactc aatttacaag ctaaaatgcg acagaaacga aaatattctt ctcataaagg
      541 agacgttggc aagaaggcag agaatctcat tcaaggccaa tttgaaggct ctaaaacaat
      601 ggaaaagtgc tacacagatg tgacagaatt tgccattcca gcaagtactc aaaagcttta
      661 cttatcacca gttttagatg gctttaatag cgaaattatc gcctat
//
LOCUS       DAVMGZ010000234          691 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000234 DAVMGZ010000000
VERSION     DAVMGZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.236"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..656
                     /locus_tag="VYQ93_002035"
     CDS             <1..656
                     /locus_tag="VYQ93_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3336644.1"
                     /translation="FVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQS
                     NQIILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDD
                     KALEDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVA
                     ETLLLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
BASE COUNT          270 a           91 c          106 g          224 t
ORIGIN      
        1 attttgtttt tttattacac gagaattgtt gttatcttgc tgtttccatt ggttttaaaa
       61 aagagctaat agaaaaacat ttagcatcta ttaacattaa tccagaatct ttttattcaa
      121 ccttgcttca atcgaatcaa ataatactta caaaggcctt agaaagagtg gcaatggaaa
      181 tattaaattg taagatggac gctcctgccg ctgatttttt ctttaaagca aaggcaaatg
      241 aatggataag tatagtaatt gacacatact taaatagaaa aaaatataaa attgaaattg
      301 atgatgataa agcacttgaa gatgtagcaa gattcttaga tgatcatttt gcaacgaatg
      361 taaatcaagg cacgcttgaa aaaatatcta aaatgagtgg tactaaatta aaaaatttat
      421 ttaaggaaaa atattgtcaa agcattacag agtacactca aagaaaaaga atgaatgtag
      481 ctgaaactct tcttttaaac actgaactac ctataaaaga gatagcagaa tctgttggat
      541 attcatctgc cagcaaattt tctatctatt ataaaagata caagggaaaa cttccaagtg
      601 aagtccgtaa tttagcttgc aaacatcaca attttaaatg cgattattgt gattaaagta
      661 attttttaca agccatgttt ttatatgaat t
//
LOCUS       DAVMGZ010000235          662 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000235 DAVMGZ010000000
VERSION     DAVMGZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.237"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..597)
                     /locus_tag="VYQ93_002036"
     CDS             complement(<1..597)
                     /locus_tag="VYQ93_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV3336645.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHIDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDE"
BASE COUNT          224 a          131 c           82 g          225 t
ORIGIN      
        1 ttcatcaagt tcctcttgat acatttttaa ttctttagtt tttcccatat aactaaccat
       61 actactatca cttacatatg ggaagtcctc ataatatatt actttataac gcataaattc
      121 aagcgccctt ccaatactat tcacaaaaac atgatcaata tggtcaccaa gtgaaagcgg
      181 acaatatacg acacatttgt cgtctaaatg cattaacagc tcttttatga tatcattctt
      241 taatgtgtcc tcatttttta attcactata gatatgacgg tatagaaaat tgccatttct
      301 atctttccta tagagacatt catagtacga taagtgtcta aaatcacact gtagacgttc
      361 acaagctaac ctgtcttctt tcttcctttc ttcaatcgga tatttcccaa ggttacacaa
      421 cttatgaaat tgcttagcag agggctgtag ctgttggctc aaagggtaac cagaaaatat
      481 agtaataaca agtacaattt ctccttctga agttaatttt gaaatataat caccacagga
      541 aaaaattgcg tcatctaaat gtggagataa aaagatatac ttagtattgt tactcataac
      601 cattccctct acaatttatc taaaaattca ctaagtgtct gattaaattc cacatcatca
      661 aa
//
LOCUS       DAVMGZ010000236          656 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000236 DAVMGZ010000000
VERSION     DAVMGZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.238"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..309
                     /locus_tag="VYQ93_002037"
     CDS             <1..309
                     /locus_tag="VYQ93_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3336646.1"
                     /translation="EQVGLSVKDFPNNILDYYVGDDGRLLSGGQKQKITLARGLIRNK
                     KIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIVTHAPHPELKQYFTKIYQFPKDFI
                     "
BASE COUNT          233 a           82 c          126 g          215 t
ORIGIN      
        1 gagcaagttg gtttgagtgt aaaagatttt cctaataaca ttttagatta ttatgttggt
       61 gatgatggga gattactgtc aggagggcag aaacaaaaaa ttactttagc tagagggcta
      121 attagaaata agaaaatagt attaattgac gagggaactt ctgctatcga taggagaact
      181 tcgttagcga ttgaacgtaa gatattagat agagaggatt tgactgtcat tattgttacc
      241 catgctccgc atccggaact taaacaatat tttactaaga tatatcaatt tccaaaggat
      301 tttatttaag aatatagggg agggaaacat gagattctat tgtgaaactt atgaggtaat
      361 tactgaagag aaaaaagtat cattttttta tgtagatgag aaacccttaa ggtatctgtt
      421 atttgactat atatttctac tagttctagc taaaaaaaat atcccaatct tatgaactgc
      481 accccaaaag ttagatttat tctgtctaac ttttggggtg cagttcattt tgcatagttt
      541 tggcattcat cctagatatc catataaaat gtctgagatt gttgaaatgt ctaaaaaaat
      601 atcagatcta aacgataaaa aagaatttca caaaaagaac tgtgagttga ggtttc
//
LOCUS       DAVMGZ010000237          650 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000237 DAVMGZ010000000
VERSION     DAVMGZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.239"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            231..605
                     /locus_tag="VYQ93_002038"
     CDS             231..605
                     /locus_tag="VYQ93_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV3336647.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          222 a           81 c          141 g          206 t
ORIGIN      
        1 aaaaaaaata aattttgaaa tagtattagt gagttcgcaa tcatttattg ctagtggttt
       61 agaactgtta agtttcaaac aataattgat tttaaacctt aggtaaccta taaaaacaaa
      121 taaggtagag aaaggatatc ttatctaggt agctaagtga taaaaacaca gtcgtctatc
      181 acttgttagg aaacgatcct aactatgctt aataaaaaag gagaaaataa atggaactcg
      241 tattaccaaa taattatgtt gctcttgagc aagaagagat gatgtatctt gatgggggat
      301 tttctattct gagatggcct gttgcaacag ccattaatat agcttttaat ggtgttttag
      361 gtggaggagc aatcagtcta gttagaaatt atattcgtaa ttatggtttg cggcgagtta
      421 caagcgcaat tgctggagca gctgcaagat atgttggggt acgagttgca aatagagtgg
      481 caggatttgc actgtctgct attaatggat ttgcagcttg gatgtcaatt ggcgatgcta
      541 ttacaacaat ctgggccaac aatgatgtaa ataggagaga cccaaattta aacgccttgt
      601 ggtaactaga tatgggtatt aaaatggaaa aaatttttgt tattattttt
//
LOCUS       DAVMGZ010000238          638 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.240, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000238 DAVMGZ010000000
VERSION     DAVMGZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.240"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>638
                     /locus_tag="VYQ93_002039"
     CDS             <1..>638
                     /locus_tag="VYQ93_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEV3336648.1"
                     /translation="LKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEKLH
                     KRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHTTT
                     IDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGYRL
                     AMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDML"
BASE COUNT          208 a           94 c          125 g          211 t
ORIGIN      
        1 ttaaaaattt ataataggga atatagttta caatgggatg gaatatacta cctagcacta
       61 attgattatc caaatattca agagtgggaa ttagaaaaaa ttgctaaatt tatagcttac
      121 gaaaaacttc ataaacgtca aacaagtatt gagtgtgctg attcttgttt aaaaaaagaa
      181 attttagatt acatctgtca gcatcccttt ctgccaccat ttacccctac agataaaaga
      241 gtagcctcga cttatgatct acataagagg ttagtgactt cagactactg tagtcatact
      301 acgactatag atgcagcgat ttctatcttt aaaacaggtc agctcttatc tgctgtgaaa
      361 gcctttaggc gagatgctga agagttggtt ttggatagta gaaatgctgc atcggatccg
      421 atagattatt ttgattatgt catgttaggg tggtcaaata caagttctgg ttatcgatta
      481 gcgatggagc gtttattagg tcgagctcct tcagagaaag aattacaaga caagtttatt
      541 cctggagtga gttttcattt tatctataca gatttgatta aagttactgg ttatattttt
      601 gatggctacc atgttgcaaa aattaaggac atgttgaa
//
LOCUS       DAVMGZ010000239          630 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000239 DAVMGZ010000000
VERSION     DAVMGZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.241"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            273..572
                     /locus_tag="VYQ93_002040"
     CDS             273..572
                     /locus_tag="VYQ93_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV3336649.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          236 a          122 c          107 g          165 t
ORIGIN      
        1 ttgcaaacaa agcatacgaa cctttagtaa aatcatttcc atgaaactag aatagagccc
       61 tcttagcaaa aatcattatt ttaatttatt tctaatcact ccttgacata aataactctc
      121 accaataaaa gactatgtct taaaaaaatg gtataataaa atcaatactt gggcttgatg
      181 gctatgctac taataacaat taggagagaa aatcaggcac ttgttaacaa caaggattat
      241 ccccttgaga tgaaaggaac tttagaaatc ttatgatgaa catgcaaaac atgatgcgtc
      301 aagcacaaaa acttcaaaaa caaatggaac aaagccaagc tgaacttgct gctatgcaat
      361 ttgttggcaa atctgctcaa gatcttgtcc aagcgacctt aactggcgat aagaaagttg
      421 tcagcattga tttcaatcca gctgtcgttg acccagagga ccttgagact ctttctgata
      481 tgaccgttca agccatcaac tctgctcttg aacaaatcga tgaaactacc aagaaaaaac
      541 tgggtgcttt cgctgggaaa ttacctttct aaaaacaagg agctagaaca atgcttgtcg
      601 ataacaaagg ctaagaaagg tgcaaaaatg
//
LOCUS       DAVMGZ010000240          593 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000240 DAVMGZ010000000
VERSION     DAVMGZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.242"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..300)
                     /locus_tag="VYQ93_002041"
     CDS             complement(<1..300)
                     /locus_tag="VYQ93_002041"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEV3336650.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKA"
     gene            complement(290..>593)
                     /locus_tag="VYQ93_002042"
     CDS             complement(290..>593)
                     /locus_tag="VYQ93_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEV3336651.1"
                     /translation="QEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDLLLY
                     KTLDQGRYNVEGPLVLARYLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYGS"
BASE COUNT          196 a          103 c           84 g          210 t
ORIGIN      
        1 tgctttctct acattatcct ctatacgttt taatcctaaa tttaaacctt gtttatgagt
       61 cggtaataaa ataatagcag gcacaacttg ggaatcataa tggcgaattg tatctaatat
      121 cattgaagca atgtcttcag tgatataaat gacaccataa tcagattgag ctaattttct
      181 tagtgtattt atagcttctt gttcttggta ggcaggaaat atatcaaacc caatcatgct
      241 aaatggtaaa ataatatcac ggctaccaat tatgccaatt ttatacttat tagcttccat
      301 aatgtgggcg tatcctttct tttattgatt caaagggaat tgtattttga agagctgata
      361 tgatcattct gagattcttt acttcaaact cacatcccaa taaatatcta gcaagaacta
      421 acggcccctc tacattgtac cttccttgat ctaaagtttt atacaatagt aaatcacata
      481 aaaattctaa ctcagaagat gaaattgttg cgtcctgcat cttcaattca taagttgaaa
      541 agattgaatc taagcttgga tttattttat tgaaccacac aaatatttct tga
//
LOCUS       DAVMGZ010000241          579 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000241 DAVMGZ010000000
VERSION     DAVMGZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.243"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(344..556)
                     /locus_tag="VYQ93_002043"
     CDS             complement(344..556)
                     /locus_tag="VYQ93_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV3336652.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKN
                     LFLNFINPEEWDTISFDSDFMPFQQS"
BASE COUNT          204 a          101 c           77 g          197 t
ORIGIN      
        1 attctaaact aagtgattca atccttttta agctatctaa acacattaaa gtcatcaagc
       61 cttaataaac tgaaagagcc tgggtaacaa ctcaatttca gaagaaaaaa agtaaatctt
      121 cccacaataa aaagcatagt atcaggtgtt tcaacacctg atactatgct tttttctata
      181 tcaaagactt tttgcccaat tcctcaaagc tatgcattga gaaatctgcg actcgcttcc
      241 tagtttaaag ttgctctttg attttcattg agtataagca acgtgattag ataagagttt
      301 tacagagtac taaatataag atttaacttt tttgagaaat tggttacgat tgttgaaacg
      361 gcataaaatc tgaatcaaag gagatagtat cccattcttc aggattgata aaatttaaaa
      421 atagattttt aaattcttcc agctcatcat ctgaatgaca aatccattct ttaccagtcg
      481 agacatacca ttttccaaga cttttcaatt cttcactcgt caaacacggt atttgagagc
      541 atttttcgaa atttataata taaacttttt ttcataaat
//
LOCUS       DAVMGZ010000242          572 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000242 DAVMGZ010000000
VERSION     DAVMGZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.244"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(197..493)
                     /locus_tag="VYQ93_002044"
     CDS             complement(197..493)
                     /locus_tag="VYQ93_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEV3336653.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
BASE COUNT          209 a          105 c           71 g          187 t
ORIGIN      
        1 gtttcatatt ataacgattc aaagtagcgt aatctactac caaaaaaagt aaaaataatt
       61 tcccttgaaa aggaagatag cttttaaaat agattgacaa acttatgata acaaaaaaca
      121 aaagggttat cccaataaaa ttaagataac ctaaaatact ttcacattct tttttagaaa
      181 gtattttcat ctagttctac catagagctg ctttagcagc acctttcttt ttcaatatat
      241 aaagaactgt tgctgcaata cccgaaccaa tagaacctac tcctgtgatt gcacctacaa
      301 ttgaaattac tgctgtaact gtactataag cactaatcaa attgactaca gtagcagcag
      361 cttttgtcga aattcctaat gtcccagcca acataggaaa agaaacaaca cctaagctta
      421 accaactaat gactgctaac attgctgaaa caataagaaa tttattatga aattccattc
      481 ttttagattt cattttagtg tactcctttt gtatattatg tatgttttat tgagagttaa
      541 ttcgcacatg ttgtattcct aactctcttc aa
//
LOCUS       DAVMGZ010000243          567 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000243 DAVMGZ010000000
VERSION     DAVMGZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.245"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..285
                     /locus_tag="VYQ93_002045"
     CDS             <1..285
                     /locus_tag="VYQ93_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV3336654.1"
                     /translation="KNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQ
                     VQLLPFHQFGENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            360..557
                     /locus_tag="VYQ93_002046"
     CDS             360..557
                     /locus_tag="VYQ93_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV3336655.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSDISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
BASE COUNT          202 a          104 c           72 g          189 t
ORIGIN      
        1 aaaaacattc attatgcttt ttctcaaaat aaaactatcg ttttaagaat cccagttatt
       61 cctaatttta acaatagttt agaggatgca gaaaaattcg ctactctatt taactcatta
      121 aatatcgacc aagttcaact actccctttt catcaatttg gtgaaaacaa atatcgttta
      181 ttaaatcgga aatatgaaat ggatggagtc aacgcacttc atcctgaaga tcttattgat
      241 tatcaaaagg tatttctgaa ccaccatatt aattgttatt tctagtttat ttccttgaaa
      301 tgctctagct atttgcagat aacaagcatc tatataaggt tatcaaagaa gacttcccca
      361 tgactctaaa tgaatatatc ttacaatatc gcctgaaaca agctatagat aagatggctg
      421 aatctcccaa ctcctcttta agcgatatct ctgaccaagt tggattttca gactataaat
      481 attttgccaa agtatttaaa aagtatctcc atatttcccc aaagaaattg aaatcactcg
      541 gaagaatagt aaaataggct attcttc
//
LOCUS       DAVMGZ010000244          535 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000244 DAVMGZ010000000
VERSION     DAVMGZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.246"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..535
                     /locus_tag="VYQ93_002047"
                     /pseudo
     CDS             1..535
                     /locus_tag="VYQ93_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          192 a           96 c           99 g          148 t
ORIGIN      
        1 aaaccaccaa gtaaaaggaa caaaaccaag aaaggttgat agagatagac ttaaaaacta
       61 tcttactgac aatccagacg cttatttgac tgaaatagct tctgaatttg cctgtcatcc
      121 aactaccatc cactatgcgc tcaaagctat gggctacact cgaaaaaaag aaccacacct
      181 actatgaaca agacccagaa aaagtagcct tatttcttaa aaattttaat agtttaaagc
      241 acctagcacc tgtttagatt gatgaaacag gattcgatac ttatttttat cgagaatatg
      301 gtcgctcatt aaaaggtcag ttaagaagag gtaaagtatc tggaagaaga tatcagagga
      361 tttctttggt tgcaggtcta acaaatggtg agttaatcgc tccaatgact tacgaagaga
      421 tgatgacgag cgactttttt gaagcttggt ttcagaagtt tctcttacca acattaacca
      481 caccatcggt tattattatg gataatgcaa gattccatag aatgggtaag ttaga
//
LOCUS       DAVMGZ010000245          526 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000245 DAVMGZ010000000
VERSION     DAVMGZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.247"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            161..514
                     /locus_tag="VYQ93_002048"
     CDS             161..514
                     /locus_tag="VYQ93_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV3336656.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLAGAAGRIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
BASE COUNT          175 a           68 c          126 g          157 t
ORIGIN      
        1 ttatttgaat taagtaaact gttaagcttc aagcagtaac tggttttaaa tatttaaaat
       61 aagataaaga aaggaagtct aatttaggta actaaacaaa gcctgcgatc acttgttagg
      121 aaaccatcct aaccatgctt aataaaaaag gagaaaataa atggaacttg tattaccaaa
      181 taattatgtt gtgattgatg aagaagagat gatgtacctt gatgggggag cttatttaag
      241 caagcgtgct tgtcaaggaa tttgcgcagc tttagctatg agtccaggaa cttttatagc
      301 attagctgga gctgcagttt taaccaaaaa actaataaac tatattaaag ttggaggcct
      361 tggaggttgg cttattggtg cagcagcagg tgtattggct ggggcggcag gaagaatagc
      421 ttactgtatt ggatatggtg ctcttaatag aggttgtgat attagcggga acccttatcc
      481 ttgggatgga ttcatatctg cgacagtaag atgagtaatg tagata
//
LOCUS       DAVMGZ010000246          525 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.248, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000246 DAVMGZ010000000
VERSION     DAVMGZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.248"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>525
                     /locus_tag="VYQ93_002049"
     CDS             <1..>525
                     /locus_tag="VYQ93_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV3336657.1"
                     /translation="LDVVGVQDFKKEFCIPNNKKIICYVERLDPEKRPDRFLEFAEKL
                     FLVREDVIFIMAGNGSMWAALKEKICHLKCRDNFRLLGEIYPATIVYQISDLLYIPSD
                     TEGIPMCVLESMSQGTPVLASNVGGLSEIIEHRVDGFLFEKEDVEGVCACANFLLNDS
                     EYLKYIGENSKSKI"
BASE COUNT          176 a           50 c          116 g          183 t
ORIGIN      
        1 atttagatgt agtaggtgtt caagacttta aaaaagaatt ttgtattcca aataacaaaa
       61 aaatcatttg ttatgttgaa aggttggatc cagaaaaaag gccggataga ttccttgaat
      121 ttgcagaaaa attattttta gtcagagaag atgtgatttt tattatggct ggaaatggta
      181 gtatgtgggc tgctttgaaa gaaaaaattt gccatttgaa atgtagagat aatttcaggt
      241 tacttggtga aatctatcca gctactatag tttaccaaat atcggattta ttgtatattc
      301 cttctgatac agaaggaata cctatgtgtg ttttagagtc aatgtcacaa ggaactccgg
      361 ttttagctag taatgttggc gggttaagtg aaattattga acatagggtt gatggatttt
      421 tatttgagaa ggaagatgtt gagggagtgt gtgcttgtgc taatttttta ctcaatgatt
      481 ctgagtattt gaaatatata ggtgagaata gtaaatcaaa aataa
//
LOCUS       DAVMGZ010000247          524 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.249, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000247 DAVMGZ010000000
VERSION     DAVMGZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.249"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..415)
                     /locus_tag="VYQ93_002050"
     CDS             complement(<1..415)
                     /locus_tag="VYQ93_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV3336658.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVD"
BASE COUNT          169 a           91 c           78 g          186 t
ORIGIN      
        1 aatccacata ttgaattaaa tcaataaatt tttcatgatc aacaaaggca gtagtttcaa
       61 tggcagtgtg tatgtgatgt tctttagctg attttaagat ggctttagca aattcaaact
      121 gagcaaatat ttcacctcct gataaagtta aacctccacc ggattcttcg taaaattctt
      181 tgtcttttaa tacctctgta ataatttctt ctacagtctt ttcttcaccg actaaggtga
      241 atttctctcg ttgagcatct ttcatttttt caggtttcat tctttgagat tcaggattag
      301 aacaccatgg acagcgcaga ggacatcctt ttaaaaaaac agttgtacga atacccggac
      361 cgtcatgaat tgaaaagtgt tgaatattaa aaataattcc tttagatatt tccataagag
      421 ttcctcctct tcataagtct atcctatcac aaaaacgaaa gaaaaacaat tacgaacgaa
      481 aggttaattt gttgtttttt gctttttatt ttggtagaat aaaa
//
LOCUS       DAVMGZ010000248          507 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.250, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000248 DAVMGZ010000000
VERSION     DAVMGZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.250"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..415
                     /locus_tag="VYQ93_002051"
     CDS             <1..415
                     /locus_tag="VYQ93_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV3336659.1"
                     /translation="EEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADNLYK
                     PSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSIRQNAMETLTG
                     LKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIP"
BASE COUNT          192 a           85 c          107 g          123 t
ORIGIN      
        1 cgaggagaaa cctcagacag aaaaacctga ggaagataag gaacatgatg aagtaagtga
       61 gccaactcac cctgaatctg atgaaaaaga gaatcacgct ggtttaaatc cttcagcaga
      121 taatctttat aaaccaagca ctgatacgga agagacagag gaagaagctg aagatactac
      181 agatgaggct gaaattcctc aagtagagca ttctgttatt aacgctaaga tagcagatgc
      241 ggaggccttg ctagaaaaag taacagatcc tagtattaga caaaatgcta tggagacatt
      301 gactggtcta aaaagtagtc ttcttctcgg aacgaaagat aataacacta tttcagcaga
      361 agtagatagt ctcttggctt tgttaaaaaa aagtcaaccg gctccgatac cgtagtaaaa
      421 tgaatggagc atattttatg gagaagtaac ctttcgtgtt acttctcttt tttagaaaaa
      481 cgtaacagaa acttgacaaa agtaatt
//
LOCUS       DAVMGZ010000249          505 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.251, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000249 DAVMGZ010000000
VERSION     DAVMGZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.251"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            22..>505
                     /locus_tag="VYQ93_002052"
     CDS             22..>505
                     /locus_tag="VYQ93_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV3336660.1"
                     /translation="MGIKNSRGKYISFLDDDDEYMPDRILKLMACFKKSRMKNLALVY
                     SYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQ
                     DGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINADEEYYNYCRKYFNL
                     L"
BASE COUNT          186 a           55 c          103 g          161 t
ORIGIN      
        1 ggtgcaacta atgccagaaa cataggtatc aaaaattcta gaggtaagta tatatcattt
       61 ttagacgatg atgatgaata tatgccagat cgaattttaa agttgatggc ttgttttaaa
      121 aagagtagaa tgaagaattt agctttagtt tatagttatg gaataataat ttatccaaat
      181 ggtacacgag aagaggagaa gaccgatttt gttggaaatc ccttgtttgt tcaaatggtt
      241 cacaatatag caggtacgtc attttggttg tgtaaaaaag aggtgctaga attaattaat
      301 ggttttgaga aaatagattc acatcaggac ggtgttgttt tattaaaact acttgctcaa
      361 ggataccaaa ttgatatagt gcgagaattc ttggtgaatt actacgctca cagtaaagaa
      421 aacggtatca ctggagtgac acaaaaaaca attaatgcag atgaagaata ttataattac
      481 tgtaggaaat attttaattt attga
//
LOCUS       DAVMGZ010000250          504 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.252, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000250 DAVMGZ010000000
VERSION     DAVMGZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.252"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..504
                     /locus_tag="VYQ93_002053"
                     /pseudo
     CDS             1..504
                     /locus_tag="VYQ93_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          166 a           90 c           96 g          152 t
ORIGIN      
        1 acaatccaga cgcttatttg actgaaatag cttctgaatt tggctgtcat ctaactacca
       61 tccactatgc gctcaaagct atgggttaca ctcgaaaaaa agaaccacac ctactatgaa
      121 caagacccag aaaaagtagc cttatttctt aagaatttta atagtttaaa gcacctagca
      181 cctgtttaga ttgacgaaac aggattcgat acttattttt atcgagaata tggtcgctca
      241 ttaaaaggtc agttaataag aggcaaagta tctggaagaa gatatcagag aatttctttg
      301 gttgcaggtc taacaaatgg tgagttaatc gctccaatga cttacgaaga gacgatgacg
      361 agcgactttt ttgaagcttg gtttcagaag tttctcttac caacattaac cacaccatcg
      421 gttattatta tggataatgc aagattccat agaatgggta agttagaact cttgtgtgaa
      481 gagtttgggt ataacttttt acct
//
LOCUS       DAVMGZ010000251          494 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.253, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000251 DAVMGZ010000000
VERSION     DAVMGZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.253"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>494
                     /locus_tag="VYQ93_002054"
     CDS             <1..>494
                     /locus_tag="VYQ93_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3336661.1"
                     /translation="QIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLLRC
                     LIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVKNFDEE
                     KAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKTSPFVFFDEPSSGMDYSNMI
                     KISE"
BASE COUNT          191 a           63 c           95 g          145 t
ORIGIN      
        1 atcagataaa aaatcttagc tacaaattta ctgatgatga gtgtttaagc ttaaaagata
       61 tttcattcaa gcttgggaaa atttatggca taataggatc caacggacga ggaaaatcaa
      121 cgcttttaag atgtttaata ggtcttgaga aaaaatcaaa agaagaaatt tattttaagg
      181 gagagaagct atctaaaaaa gaaagactca aaaactcttc acttgttatg caagatgtaa
      241 atcatcaatt attcacagat gaagtattca acgagcttag attaggagta aagaattttg
      301 atgaagaaaa ggcgaaaatc attttaaaag atttaggcct ggacgaattt attgaaaggc
      361 atccgatgag tttatcagga gggcaaaagc aaaggcttgc aatagcatct gttatgtgca
      421 agacttctcc atttgtcttt tttgacgaac cttcaagtgg tatggattat tccaatatga
      481 taaaaatatc tgaa
//
LOCUS       DAVMGZ010000252          492 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.254, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000252 DAVMGZ010000000
VERSION     DAVMGZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.254"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>492
                     /locus_tag="VYQ93_002055"
     CDS             <1..>492
                     /locus_tag="VYQ93_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336662.1"
                     /translation="DAFRFGTDEKEDKDTYQATFETNHPAIKHFFGPAGNNVVHNSNG
                     AYATGDAFYYMAYRMLDKDGAVTYTHEMTHNSDREIYLGGYGRRNGLGPEFYAKGLLQ
                     APDHPNDPTVTINSILKYDQSEESTRLQVADPTQRFGSVDDLNKYMHNMFDVIYMLEI
                     LEG"
BASE COUNT          181 a           77 c           93 g          141 t
ORIGIN      
        1 tgatgcattt agatttggta cagatgaaaa agaagataaa gatacatatc aagcaacttt
       61 tgaaactaat catccagcta taaaacattt cttcggtcca gcgggaaata atgttgttca
      121 taattctaat ggagcatatg ctacagggga tgcattctat tatatggcat atcgcatgct
      181 tgataaagat ggtgcggtaa cttatacaca cgaaatgaca cataactcag atagagaaat
      241 ttatcttgga ggctatggaa gacgaaatgg acttggacca gagttttatg ctaaaggact
      301 gctacaagct cctgatcatc ctaatgatcc aacagtcact attaattcca tattaaaata
      361 tgatcaatca gaagaatcaa caaggcttca agtagcagat ccaactcaac gatttggaag
      421 tgttgatgat ctaaataagt atatgcataa tatgtttgat gtaatataca tgcttgaaat
      481 actagaaggt aa
//
LOCUS       DAVMGZ010000253          483 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.255, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000253 DAVMGZ010000000
VERSION     DAVMGZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.255"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..423
                     /locus_tag="VYQ93_002056"
     CDS             <1..423
                     /locus_tag="VYQ93_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3336663.1"
                     /translation="NQERFRCLIQELDLTQSINKKVKTYSLGTKQKLALLLTLVTEPD
                     ILILDEPTNGLDIESSQIVLAVLKKLALHENVGILISSHKLEDIEEICERVLFLENGL
                     LTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFWDIV"
BASE COUNT          173 a           55 c           90 g          165 t
ORIGIN      
        1 aatcaagaaa gatttagatg tttgatccaa gagttagatt tgactcagtc tattaataaa
       61 aaagtaaaga cctattcttt gggtacaaaa caaaaattag ctttgcttct aactctcgtt
      121 acggaacctg atatattgat tttagatgaa ccgactaatg gtttagatat tgaatcatca
      181 caaatagttt tagcggttct aaaaaaatta gctttacatg aaaatgtggg aattttaata
      241 tcgagtcata aattagaaga cattgaagaa atttgtgaga gagttctttt cttggagaac
      301 gggcttttga catttcaaaa agtaggaaaa gatagtcata atttcttgtt tgagatagct
      361 ttttcatcag ctacagatag agacattttc attaccaaac aagaattttg ggatattgtt
      421 taggaagagg gattgagaat tactatgtct gggaatattc aaaatagtga gctttttaaa
      481 ttt
//
LOCUS       DAVMGZ010000254          474 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.256, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000254 DAVMGZ010000000
VERSION     DAVMGZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.256"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(144..>474)
                     /locus_tag="VYQ93_002057"
     CDS             complement(144..>474)
                     /locus_tag="VYQ93_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV3336664.1"
                     /translation="EEKPQTEKPEEETPREEKPQSEKPESPKPTEEPEESPEESEEPQ
                     VETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLL
                     ALLKESK"
BASE COUNT           93 a          101 c           82 g          198 t
ORIGIN      
        1 agaggtatac tattctagtt tcaatctact atattttgca cattttcatg aaaaaaatga
       61 gaactagaac tcacattctg ctctcatttt tcgttttccc gttctcctat cctgttttta
      121 ggagttagaa aatgctgcta cctttactta ctctccttta ataaagccaa tagtttttca
      181 gcttctgcca taatagtatt gttgtcctgg gtgccaaata gtaaattatt ttttaatcct
      241 gtgagagtct ctttggcatt ggacttgata attggatcct ggatttttcc aagtaaatct
      301 tcagcctctc tcagtttttc ttcaaccttt tcagtctcga cctgaggttc ttctgattcc
      361 tctggtgatt cttctggttc ctctgttggt tttggagact ctggtttctc gctttgcggt
      421 ttctcttctc gaggggtttc ttcctcaggt ttttctgtct gaggtttctc ctcg
//
LOCUS       DAVMGZ010000255          449 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.257, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000255 DAVMGZ010000000
VERSION     DAVMGZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.257"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>449
                     /locus_tag="VYQ93_002058"
     CDS             <1..>449
                     /locus_tag="VYQ93_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV3336665.1"
                     /translation="FIYDLAVIKLPETTGREHVELVKNYSKVNLNDKLNVHGYPAGKY
                     THLKDATVEMEQEYANNTYGVQYQGGNPGMSGGGIFNANGEVIGVHQNGAQNRSGGLI
                     LSPTQLAWIKSIIAGNEIPPVYDELYRHKDEKKDDAKDEKEVIKKLEL"
BASE COUNT          173 a           59 c           94 g          123 t
ORIGIN      
        1 tttatctatg atttagcagt gattaagcta cctgaaacaa ctggcagaga acatgtagaa
       61 ttagttaaaa attattcgaa agtaaatctt aatgacaaac taaatgttca tggatatcca
      121 gctgggaaat atacgcactt aaaagatgct acggtagaaa tggaacaaga atatgcgaat
      181 aacacctacg gtgttcaata tcaaggtgga aatcctggta tgagtggtgg aggaatattt
      241 aatgctaatg gtgaagttat tggtgtgcat caaaatggag cacaaaatcg ctcaggcgga
      301 ttgattttat ctcctacaca attagcttgg attaagagta ttatagcagg taatgaaatt
      361 ccaccagtat acgatgaact ttatcgtcat aaagatgaga aaaaagatga cgctaaagat
      421 gaaaaagaag ttattaagaa attagaact
//
LOCUS       DAVMGZ010000256          440 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.258, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000256 DAVMGZ010000000
VERSION     DAVMGZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.258"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(29..379)
                     /gene="tnpB"
                     /locus_tag="VYQ93_002059"
                     /pseudo
     CDS             complement(29..379)
                     /gene="tnpB"
                     /locus_tag="VYQ93_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          141 a           93 c           82 g          124 t
ORIGIN      
        1 tcactatact aaaaagaaag tcctcatttc aatagaaatc acgactttct gatgaattta
       61 tttttggagt gatagaaaag cccttcataa gccagtctac ttgttcaggt gtgagagctt
      121 tgacatcctt ttctgtactg ggtcaagtca gtctgccgtt ctcaaagcgt ttatatagta
      181 gccaaaatcc ttgaccatcc cagtaaaggg ctttaaagcg gtctttacgt ccaccacaaa
      241 agagaaaaac ttgaccggag aaaggatcta attcaaagtg ggttttaacc agataagcca
      301 gtgaatcaat gccttgcctc atatccgttt tcccacatac gagatagacc tgccctaggc
      361 tagatagatg aattgtcata gagcaacacc ttatccaaaa gctgttctac catttcgaga
      421 ttgagagatt gaaaaaaact
//
LOCUS       DAVMGZ010000257          424 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.259, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000257 DAVMGZ010000000
VERSION     DAVMGZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.259"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          154 a           47 c           81 g          142 t
ORIGIN      
        1 ttaattagaa aaaaattttt aaagtcttcg gagaagaaaa cagaaaaatc tgttaaaaaa
       61 taatcaaaac tataaatgat gaatctgaat caaaattatt ttgcgcatgt aaataggagt
      121 cttatagtaa cgagtcaaaa aaggagtaac tatgaatcgt aatttagaac ggtgttatct
      181 attctgacta ggaatagatc ataccagagg tggcttagaa atagcgggga cattagaaat
      241 tgaaggaact ttaaatagga tgtcgtaaat gttgctatca atgatttatt tgtcccaagc
      301 ttgcctaggg tgtcagtaaa aaatcatttt cctttcatat catattttta gtaggtagga
      361 tgtttgttct gtctattttt tgccaaaaaa agtgcagtcg aagtgtttat ttggagtttt
      421 atat
//
LOCUS       DAVMGZ010000258          421 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.260, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000258 DAVMGZ010000000
VERSION     DAVMGZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.260"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          156 a           65 c           65 g          135 t
ORIGIN      
        1 ctaaaattaa tttattaaac atatctcata agcatatcta ttataggtaa gacaatatta
       61 agaaagacgg ttaaaaaaca acctgactcc actggaattc aggaatcagg tcgcttaaga
      121 ttttatatta ttttactgtc tacttgacag ggagctgttc accaatggga atctatagct
      181 tttttgtgac tgagaactat tttatctcag acttcttcaa cacaccttat tattgggcac
      241 ttacggaagg ttaatgaatt atttaaaata aaagtaaaga gaagcaaaca gacactctat
      301 agctgttaga cgctttttag agtaataaca atttgataaa gtgaatacaa cttgcttaat
      361 taagcgtaag aaaccagaat cataaaatca attactttga agtcatgact cacgttgtat
      421 t
//
LOCUS       DAVMGZ010000259          409 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.261, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000259 DAVMGZ010000000
VERSION     DAVMGZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.261"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            131..328
                     /gene="blpI"
                     /locus_tag="VYQ93_002060"
     CDS             131..328
                     /gene="blpI"
                     /locus_tag="VYQ93_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEV3336666.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
BASE COUNT          155 a           50 c           77 g          127 t
ORIGIN      
        1 taattcaaga cgtttcgatg acaattcaag atctggatga aaaattttaa aaaacgatga
       61 tatactaaac ttatcaaagt tacaacaaga caaaaataaa aaaagaaaaa agaaaaaagg
      121 agtatttgtc atgaatacaa aaatgatgga acaattttct gttatggata atgaagaact
      181 cgaaatagtt agtggaggaa gaggcaattt aggatctgca atcggtggtt gtattggagc
      241 agtactatta gctgctgcga ctggtccgat aactggggga gcagcaacac ttatttgtgt
      301 aggttcagga attatgtcct ctttgtaaga ttatgattct taaatatagt attattatcg
      361 ctattaattt actgtcttat ttacttactt acaaaatatc aaagttatc
//
LOCUS       DAVMGZ010000260          401 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.262, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000260 DAVMGZ010000000
VERSION     DAVMGZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.262"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          122 a           87 c           66 g          126 t
ORIGIN      
        1 attaggggtg aataattggg gttttatact ttaaaatttc tcgctacctt atccactata
       61 tgctcctcgc tatcacgttt ctattcatag cctacgattt cactattgct ttctctgaca
      121 attcttattt cctgcgtcag acttaaaacg atctatcccc agaccatttt aatccgctac
      181 ctcacgatag tcaggcttgg ggagcgctat tgtattcacc ggtagtggat ccctacagag
      241 gacttacacc tcagatgcac gacatgccca tcgtataaaa aatggctcta taatatttgt
      301 agtgggtaaa tccactatag atattatgga gcctatttta ttgtagaaaa aaatcccata
      361 agatctataa tgaaaagcga caaaacaact cattagaaag a
//
LOCUS       DAVMGZ010000261          400 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.263, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000261 DAVMGZ010000000
VERSION     DAVMGZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.263"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            186..>400
                     /gene="blpU"
                     /locus_tag="VYQ93_002061"
     CDS             186..>400
                     /gene="blpU"
                     /locus_tag="VYQ93_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV3336667.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAA"
BASE COUNT          149 a           47 c           97 g          107 t
ORIGIN      
        1 tagtaaaaaa gtgattagaa aacatctttt ttaaaaatag agatgatttt gaaacaaaaa
       61 agctaattca agacgtttcg atgccaattc aagatttgga tgaaaaaaat taatagatac
      121 tgttatacta aacttgtcaa gtttgtaaca agacaaatat taaaaataaa aaagaggtat
      181 tcgttatgaa tacaaaaaag atgtcacaat ttgaaattat ggatactgag atgcttgctt
      241 gcgttgaagg tggcggatgc aattggggag attttgccaa agcaggtgtt ggaggaggag
      301 cagtacgagg tcttcagcta ggaattaaaa caagaacatg gcaaggtgca gcaactggtg
      361 ctgtgggagg agctatactt ggaggtgtgg cctatgcagc
//
LOCUS       DAVMGZ010000262          399 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.264, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000262 DAVMGZ010000000
VERSION     DAVMGZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.264"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>399
                     /locus_tag="VYQ93_002062"
     CDS             <1..>399
                     /locus_tag="VYQ93_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336668.1"
                     /translation="NSVYATNVVRRLKPEELTKLTTFNSLIEHDIITRRGYVDEATYK
                     RNGYYTINLFSPIYSALSSKIGTPGDLMGRRIAFELLAAKGYKDGMVPYISNQYEKEA
                     KAQGKVITSYGKQIGLVTDEIVLSKVFNNQ"
BASE COUNT          154 a           53 c           74 g          118 t
ORIGIN      
        1 aaattctgtt tatgcaacga atgttgtacg tagattaaaa ccagaagaac ttactaaatt
       61 aactacattt aacagtttga tagaacatga tattataaca agaagaggtt atgtagatga
      121 agcaacttat aagcggaatg gctactatac aattaatcta ttctcaccaa tttattcagc
      181 attgagcagt aaaataggaa caccaggaga tttaatggga cgacgtattg catttgagtt
      241 attggcagcc aaggggtaca aagacggtat ggttccatat atttctaatc agtatgaaaa
      301 agaagcgaaa gctcaaggaa aagttattac atcatatggt aaacaaatag gtttagttac
      361 tgatgaaatt gttctttcaa aagtatttaa taaccagta
//
LOCUS       DAVMGZ010000263          399 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.265, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000263 DAVMGZ010000000
VERSION     DAVMGZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.265"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..399
                     /locus_tag="VYQ93_002063"
                     /pseudo
     CDS             1..399
                     /locus_tag="VYQ93_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          118 a           88 c           75 g          118 t
ORIGIN      
        1 agtgctatca ctgttcaaaa atgatggttg ctgagactcc cctggtaaag aaaaatcacc
       61 aaatccctcg tatcatcaac caaaaaattg ctcaaaagtt aattgaaaag atttctatga
      121 ctgatattgc ccatcagctt tccatctcaa cttcaactgt cattcgcaag ctcaatgatt
      181 ctcactttga gcatgatttt tcgcgtcttc ctgagattat gtcctgggac gttgaaacag
      241 tccggggagt gactgtttca atcgggagat ggagatgagc ttcattgcgc aagattttaa
      301 caatctcaat atcatcactg ttcttgaggg aagaacacaa gctatcattc gaaatcactt
      361 tcttcgctac gatagagctg tccgatgtcg cgtcaaaat
//
LOCUS       DAVMGZ010000264          366 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.266, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000264 DAVMGZ010000000
VERSION     DAVMGZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.266"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            105..>366
                     /locus_tag="VYQ93_002064"
     CDS             105..>366
                     /locus_tag="VYQ93_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3336669.1"
                     /translation="MAAGYLNRVNDIWLENEENVENGLKKCSLEGRIDIKDLSFSYSK
                     DSAPVIENLNLTIEPGQRIALVGQSGSGKSTLSKILSGLYKID"
BASE COUNT          132 a           46 c           71 g          117 t
ORIGIN      
        1 taaatggaaa aattttattg ggtgaggttg tcgcatttca aaccatggca tcaattttat
       61 ttagttcaga aatctcaata tttaatgctt atacgcagta tattttggca gcaggatact
      121 taaatagagt aaatgatatc tggcttgaaa atgaagagaa tgttgaaaat ggtcttaaaa
      181 agtgttcttt agaaggtaga atagatatta aggacctatc tttctcttat tccaaagact
      241 cagctcctgt aatagaaaat ttaaatttga ctattgagcc aggacagaga attgcactag
      301 tcggtcaatc ggggtcagga aaaagtacgt tatcaaaaat actatcagga ttatataaga
      361 ttgata
//
LOCUS       DAVMGZ010000265          364 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.267, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000265 DAVMGZ010000000
VERSION     DAVMGZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.267"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>364
                     /locus_tag="VYQ93_002065"
     CDS             <1..>364
                     /locus_tag="VYQ93_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV3336670.1"
                     /translation="DDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDG
                     YIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQD
                     SGNTEAKGAEAIYNRVKAA"
BASE COUNT          117 a           79 c           85 g           83 t
ORIGIN      
        1 tggatgatat tcttgccttc ttagctccga ttcgtcatcc agaacgttta ggaaaaccaa
       61 atgcgcaaat tacctacact gatgatgaga ttcaagtagc caagttggca ggcaagtaca
      121 caacagaaga cggttatatc tttgatcctc gtgatataac cagtgatgag ggggatgcct
      181 atgtaactcc acatatgacc catagccact ggattaaaaa agatagtttg tctgaagctg
      241 agagagcggc agcccaggct tatgctaaag agaaaggttt gacccctcct tcgacagacc
      301 atcaggattc aggaaatact gaggcaaaag gagcagaagc tatctacaac cgcgtgaaag
      361 cagc
//
LOCUS       DAVMGZ010000266          344 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.268, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000266 DAVMGZ010000000
VERSION     DAVMGZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.268"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>344)
                     /locus_tag="VYQ93_002066"
     CDS             complement(<1..>344)
                     /locus_tag="VYQ93_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3336671.1"
                     /translation="IAQKLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSCLPEI
                     MSWDEYAFTKGKMSFIAQDFNNLNIITVLKGRTQAVIRNHFLRYDRAVRCRVKIITMD
                     MFSPYYDLARQL"
BASE COUNT          119 a           55 c           69 g          101 t
ORIGIN      
        1 aaaagttgtc tagctaagtc atagtaagga ctaaacatat ccatagtaat gattttgacg
       61 cgacatcgga cggctctatc gtagcgaaga aagtgatttc ggatgacagc ttgtgttctt
      121 cccttaagaa cagtgatgat attgagattg ttaaaatctt gcgcaatgaa gctcatcttt
      181 ccttttgtaa aagcatactc atcccaagac ataatctcag gaagacaaga aaaatcatgt
      241 ttaaagtgaa agtcattgag cttacgaata acagttgaag ttgagatgga aagctgatgg
      301 gcaatatcag tcatagaaat cttttcaatt aacttttgag caat
//
LOCUS       DAVMGZ010000267          328 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.269, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000267 DAVMGZ010000000
VERSION     DAVMGZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.269"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..138
                     /locus_tag="VYQ93_002067"
     CDS             <1..138
                     /locus_tag="VYQ93_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEV3336672.1"
                     /translation="QLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPGRKASLMM
                     H"
BASE COUNT          105 a           57 c           80 g           86 t
ORIGIN      
        1 cagctcctca aattgtctag tctctatgcc tattccgaat tgttttcagc actttattct
       61 aggattagaa aagtcaacct gaatcttcct gttccttatg aaccacctgg aagaaaagcg
      121 tcgttaatga tgcattaaag aacagtcgag atgaaaaaat cgtgtgacgc accaagggag
      181 gagtctgccc ttttgaggaa atctagcgag gaaaaacgat actggaacag cagaaagtaa
      241 aactgacctc atgaggagga agaaagtggc tcatgaggtc aggggttttg taagttacat
      301 ctagttgaga gaggtatgaa tgatttgg
//
LOCUS       DAVMGZ010000268          283 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.270, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000268 DAVMGZ010000000
VERSION     DAVMGZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.270"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>283)
                     /locus_tag="VYQ93_002068"
     CDS             complement(<1..>283)
                     /locus_tag="VYQ93_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3336673.1"
                     /translation="LNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEGVAYNAHYRA
                     HGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR"
BASE COUNT           95 a           51 c           66 g           71 t
ORIGIN      
        1 attcgaaaga gaagttgatt aaaccgttct aaaacaggtg tgataaagct gtctgcctct
       61 tcagaacaat aacgattacc aggacgaagc tgggcattga aacaataacc tgtcttcccc
      121 tcgaaagcat aaagaggatg atagccatga gcacgatagt gggcgttata agcaacacct
      181 tcttgcttgc cataagttgt gaaatgggta gaatcgatat ctacaatgag ttggtttagc
      241 tggtgaaact gtaaaaagaa ttcgaccaat tcaaggttga ggc
//
LOCUS       DAVMGZ010000269          272 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.273, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000269 DAVMGZ010000000
VERSION     DAVMGZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.273"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>272)
                     /locus_tag="VYQ93_002069"
     CDS             complement(<1..>272)
                     /locus_tag="VYQ93_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3336674.1"
                     /translation="NISLEIDKGIYRVEGENGSGKSTLLQLLAGLETFDSGLKNCVSK
                     DVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINTFFDN"
BASE COUNT           92 a           54 c           36 g           90 t
ORIGIN      
        1 attatcaaaa aaagtattaa tttgttcttt atcctcaaat gatagatgga tttgaaaaga
       61 atcacacaac aactgcaaat tttcttctat agtaaatgga acaaccccaa gctgattggt
      121 atcaaggtat agtacatctt tggagacaca attctttaaa ccactatcaa aagtctctag
      181 cccagctaaa agttgtagaa gggtgctctt tcctgaacca ttctctcctt caactctgta
      241 aattccctta tcaatctcta gtgatatatt tt
//
LOCUS       DAVMGZ010000270          270 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.274, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000270 DAVMGZ010000000
VERSION     DAVMGZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.274"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>270
                     /locus_tag="VYQ93_002070"
     CDS             <1..>270
                     /locus_tag="VYQ93_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HEV3336675.1"
                     /translation="KSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQA
                     PSRARKLVSQNSVLFSTVRPYLKNIAVVRELKEYLIASTAFIVLD"
BASE COUNT          102 a           40 c           43 g           85 t
ORIGIN      
        1 taaaatcaat ttattggaat tttgggcaaa ataagccaga gaaatccttt aggtatatag
       61 atacgtctag tattgataga aaaaagaaca taatcaacta caaaaatcta caatatcttt
      121 cacctgaaca agcgccttcc cgtgccagaa aattagtttc gcagaatagt gtcttatttt
      181 caacagttag accatatcta aaaaatattg ctgtagttag agaacttaaa gagtatttga
      241 tagctagtac agcatttatt gttttggata
//
LOCUS       DAVMGZ010000271          269 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.275, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000271 DAVMGZ010000000
VERSION     DAVMGZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.275"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            19..>269
                     /locus_tag="VYQ93_002071"
     CDS             19..>269
                     /locus_tag="VYQ93_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3336676.1"
                     /translation="MRIKEKTNNINGGIKNVSKHYGHSIILKDINFALNKGEIVGLAG
                     RNGVGKSTLMKILVQNNQPTSGNIISSDNVGYLIEEPKL"
BASE COUNT          108 a           29 c           52 g           80 t
ORIGIN      
        1 cgcaactttt taaaattaat gagaatcaaa gagaaaacca ataatattaa tggaggaata
       61 aaaaatgtaa gtaagcatta tggtcattca atcattctca aagatataaa ttttgcactt
      121 aacaagggtg aaattgttgg tctagcaggg agaaatggag ttggtaagag tacgttgatg
      181 aaaattcttg ttcagaataa tcaaccgact tcaggtaata ttataagcag tgataatgtt
      241 gggtatttaa tcgaagaacc aaaattatt
//
LOCUS       DAVMGZ010000272          262 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.276, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000272 DAVMGZ010000000
VERSION     DAVMGZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.276"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>262)
                     /locus_tag="VYQ93_002072"
     CDS             complement(<1..>262)
                     /locus_tag="VYQ93_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336677.1"
                     /translation="NILPDIQFQTSAVGCIITVVLISFLAFLSGCFYTFKKIRNRKSS
                     KIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDSTILAKAQIIF"
BASE COUNT           96 a           48 c           37 g           81 t
ORIGIN      
        1 tgaatattat ctgggccttc gctaaaattg tgctatcttg gaaaatttgt tgcaaagaaa
       61 gcaaccagat aacactaaaa ccagccaata gcagtattct ttttactatt gaaagaacat
      121 gccttatttt agaactcttc ctatttctaa tcttcttgaa cgtataaaag caaccactta
      181 gaaaggctaa aaatgaaatc aacactactg taatgataca tccaacagca ctcgtttgaa
      241 attggatatc aggtaatata tt
//
LOCUS       DAVMGZ010000273          258 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.denovo.277, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000273 DAVMGZ010000000
VERSION     DAVMGZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.denovo.277"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>258
                     /locus_tag="VYQ93_002073"
     CDS             <1..>258
                     /locus_tag="VYQ93_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336678.1"
                     /translation="QSIAGSPYSIGVYDRLTSPSWKYPSMVLPLLTLPEKSVFIIANI
                     STIGFGAYDRYRSKEHPAGTDLNDYVEKKAKEAAVRFRDHY"
BASE COUNT           90 a           38 c           53 g           77 t
ORIGIN      
        1 agcaatcaat agctggtagt ccatattcaa ttggagtata tgatagatta actagcccat
       61 cttggaaata tccaagtatg gtgttgccgc tattaacatt acctgaaaaa tcggtgttta
      121 ttatagccaa catttcgact ataggtttcg gtgcttatga tagatataga agtaaagaac
      181 atccagcagg aactgattta aatgactatg ttgagaaaaa agctaaagaa gcggctgtta
      241 gatttagaga tcactatg
//
LOCUS       DAVMGZ010000274        24746 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_I18
            SAMN29867923-rid17925013.guided.014, whole genome shotgun sequence.
ACCESSION   DAVMGZ010000274 DAVMGZ010000000
VERSION     DAVMGZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867923
            Sequence Read Archive: SRR20340393
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719898.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719898.1
            Genome Coverage       :: 442x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:10:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,060
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 57 of 123
            Pseudo Genes (incomplete)         :: 55 of 123
            Pseudo Genes (internal stop)      :: 40 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867923-rid17925013.guided.014"
                     /isolate="GC089790_I18"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-07"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(299..3112)
                     /locus_tag="VYQ93_002074"
     CDS             complement(299..3112)
                     /locus_tag="VYQ93_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV3336679.1"
                     /translation="MKDKREIIRARKAFRRSLKDEKKFLKKGKKEVRKQKKDSAVLDE
                     KAWKKEIKEKLEEMREASKARVKQANEDYNHILQNTPPSLLNRKELRDRRLPHARKRL
                     KIAKKQFREAKVEAKEERKESRKERKTNQKFLYGQESKAKSNFFFQGKSLEELKAKKE
                     VKAAKDNLKSTKQAYKSKKVSRKAKTFLYVLGREGGELASENEDLEGYRTLQETIRKG
                     KRYSRLSYNLGKASVKTGQATGRFTKKRLTNTKERYHHFKDGKGWKLAKDNPSSFKNR
                     YRKLKKQGLSSVRNIYQKLKAAFSFFTFTAGNPATWIVGGLVFLLLLMMSFFLGFSSA
                     SLIQQDEFELTKAYTHLTWEDAEHTRTNDKGITYYTKVDDVMGYMNFKFHDYELHKPV
                     HLFSSETYKDYLSTLWHDLNDGDDLKSMQDLYETPKYKLSKDDQEEMKELKEEGVYAS
                     MQELDNPFEGKSNEDSLTMTYRYGYYDLDGKPTLQEYILLEAKAHQTIVAPMDGVVSL
                     DGDNIILTNGKGENESRLTLYSIHNGRAIEGTRVLTGDIIGETPDDTGLKVSYQKYKN
                     KKEKLVYVNPQFYFPKVIQLQTTILPAIGQFGGDEFERAKHIYEFLKSQGASPQAIAA
                     ILGNWSVESSINPKRAEGDYLSPPVGATDSSWDDETWLAIGGPAIYSGAYPNILHRGL
                     GLGQWTDTADGSTRHTALLNYAHIKNKKWYDLDLQLDFMLHGDSPYYQSWLKDFFGNT
                     GSAANLAQLFLTYWEGNSGDKLLERQTRATEWYYQIEKGFSQTNGGQAKSDPQSLEGV
                     RGDLYDHSVPGGGDGMAYAYGQCTWGVAARMNQLGLKLKGSNGEKISIINTMGNGQDW
                     VATASSLGGETGSTPRAGAIVSFVGGTHGTPAIYGHVAFVEKVYDDGSFLVSETNYGG
                     NPNYTFRKISQADSAISFAYTVK"
     gene            complement(3124..5481)
                     /locus_tag="VYQ93_002075"
     CDS             complement(3124..5481)
                     /locus_tag="VYQ93_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV3336680.1"
                     /translation="MKRKSNTLKKQKTSMTNKKEEVKGKKEEVLPSTANTLSYQALYQ
                     NGLMQVKEDYFSQSYLLGDVNYQTVGLEDKGAIIEKYSDLINSLDDQTNFQLTIFNKR
                     LNLEKFRQSVLYEEKEDGYDSYRKELNRMMNQNLDSGENNFSAVKLISFGRKDSNPKQ
                     AYRSLSQIGEYFKSGFSEIDARFESLAGEERVNLLADMLRGEHHLPFSYRDLTRSGQT
                     TRHFIAPNLLDFKNKNYLQINDRLLQIVYVRDYGMELGDQFIRDLMQGDLELIVSLHA
                     QSSTKADAMKKLRTKKTLMESQKIGEQQKLARTGIYLEKVGHVLESNIDEAEELLKTM
                     TETGDKLFQTVFLIGVFGQDEEELKQALDTIQQVAGSNDLMIDKLPYMQEAAFNSLLP
                     FGCDFLEGVSRSLLTSNVAVNSPWTSVDLQDRSGKYYGINQISSNIITIDRSLLNTPS
                     GLILGTSGAGKGMATKHEIITTKIKESGENTEIIIVDPEAEYSVIGRTFGGEMIDIAP
                     DSQTYLNVLDLSEENMDEDPVKVKSEFLLSFIGKLLDRKMDGREKSIIDRVTRLTYQS
                     FKEPSLEEWVFVLSQQAEEEAQNLALDMELYVEGSLDIFSHKTNIQTGSNFLIYNVKK
                     LGDELKQIALMVVFDQIWNRVVRNQKLGKKTWIYFDEMQLLLLDKYASDFFFKLWSRV
                     RKYGASPTGITQNVETLLLDPNGRRIIANSEFMILLKQAKNDREELVQLLGLSKELEK
                     YLVNPEKGAGLIKAGSVVVPFKNKIPQGTQLFDIMSTDPDKMASN"
     gene            complement(5432..5791)
                     /locus_tag="VYQ93_002076"
     CDS             complement(5432..5791)
                     /locus_tag="VYQ93_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEV3336681.1"
                     /translation="MNTCVFKDISKYQHRAWLGFTTRQIIFVLPAFIVTIIVLGLNLF
                     FWQFGDWFVYGFVFAFTIPLMLFGVYKPNDLYFEHYLKYRLHFELTVPLRTITGKKGP
                     EHEKKIKYIKETKNFND"
     gene            complement(5845..6699)
                     /locus_tag="VYQ93_002077"
     CDS             complement(5845..6699)
                     /locus_tag="VYQ93_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HEV3336682.1"
                     /translation="MNLSLVSPFVYLASEKISAENLFEGFNVDLQSTVDLIKSLSSYN
                     PTVWTYMSSITKSVMQPLGVAILSVVLILEFSKMAKKIANSGGAMTFEALAPMLISYI
                     MVAVVITNTTVIVEAIIGIASHAIEQVASIVAHGGAKYDTLSGLKGSGFIGRMIVGFF
                     ALLIWLVRIVSAAMVNLLVSIRFIQLYLMIPFAPLTIPTFLSDEWKSIGIGYLKNIMV
                     YAVQGVLIFLIVSLVPLFESAGKIAVSNGAGVLQSLAIMFGSLVQAILLIIALVGSQR
                     TARSILGM"
     gene            complement(6716..6958)
                     /locus_tag="VYQ93_002078"
     CDS             complement(6716..6958)
                     /locus_tag="VYQ93_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336683.1"
                     /translation="MITHFKGFVYGVDASAMFAQAMSLLQKGLIAVGAFLVVVGIVNL
                     ATNIKDGGPGVRNAILEIVGGVMVGAAGAFVTQISI"
     gene            complement(6979..8856)
                     /locus_tag="VYQ93_002079"
     CDS             complement(6979..8856)
                     /locus_tag="VYQ93_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEV3336684.1"
                     /translation="MYSGKKFLLFSLLGILLGYLFHRLTLLYDSYTGNTLDKWTHLLM
                     EGQDEVLQSPWNVSFTGKSSAFFLLGFVMMLLVYLYLETGKKQYREGVEYGSARFGTL
                     KEKKLFYGKEFSHDTILAQDVRLTLLDKKPPQYDRNKNIAVIGGSGSGKTFRFVKPNL
                     IQMNSSNIVVDPKDHLAEKTGKLFLEHGYQVKVLDLVNMKNSDGFNPFRYIETENDLN
                     RMLTVYFNNTKGSGSRSDPFWDEASMTLVRALASYLVDFYNPPKTREQLIEESRLSQK
                     EYQNLLKRQKKEVEERKKRGRYPSFAEISKLIKHLSKGENQEKSVLEILFENYAKKYG
                     TENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNIQSVMDLTKRDTLDMKTWGKEKS
                     MVYLVIPDNDSTFRFLSALFFSTVFQTLTRQADIDFKGQLPLHVRVYLDEFANIGEIP
                     DFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLVYLGGNDEDTFK
                     FMSGLLGKQTIDVRNTSRSFGQTGSGSLSHQKIARDLMTPDEVGNMKRHECLVRIANM
                     PVFKSKKYNSTKHPNWKYLANQESDERWWNYQINPLNQRQENYLDDLRIRDLTFESSL
                     K"
     gene            complement(8856..9344)
                     /locus_tag="VYQ93_002080"
     CDS             complement(8856..9344)
                     /locus_tag="VYQ93_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336685.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVKSLLKILEWSRRQALAQDSAYK
                     IGVQKLEELLQSPYAIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFY
                     AKDRSILDNHLDHLLEKMVNDPEKLADFTMDKSLDDAIDEAKSQITFRQEGAVKQKEM
                     VR"
     gene            complement(9334..9633)
                     /locus_tag="VYQ93_002081"
     CDS             complement(9334..9633)
                     /locus_tag="VYQ93_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336686.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIVDGKAVEVTLREPYSRDLLKVVKSIKQRQKLIAYRYKEGKLLFVKKEASDVL"
     gene            10306..10620
                     /locus_tag="VYQ93_002082"
     CDS             10306..10620
                     /locus_tag="VYQ93_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV3336687.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            10636..11022
                     /locus_tag="VYQ93_002083"
     CDS             10636..11022
                     /locus_tag="VYQ93_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV3336688.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            11051..12436
                     /locus_tag="VYQ93_002084"
     CDS             11051..12436
                     /locus_tag="VYQ93_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV3336689.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            12439..12591
                     /locus_tag="VYQ93_002085"
     CDS             12439..12591
                     /locus_tag="VYQ93_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV3336690.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            12614..13819
                     /locus_tag="VYQ93_002086"
     CDS             12614..13819
                     /locus_tag="VYQ93_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEV3336691.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            13862..14083
                     /locus_tag="VYQ93_002087"
     CDS             13862..14083
                     /locus_tag="VYQ93_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3336692.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            14200..14697
                     /locus_tag="VYQ93_002088"
     CDS             14200..14697
                     /locus_tag="VYQ93_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEV3336693.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            14672..15178
                     /locus_tag="VYQ93_002089"
     CDS             14672..15178
                     /locus_tag="VYQ93_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV3336694.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            15162..17609
                     /locus_tag="VYQ93_002090"
     CDS             15162..17609
                     /locus_tag="VYQ93_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV3336695.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            17612..19789
                     /locus_tag="VYQ93_002091"
     CDS             17612..19789
                     /locus_tag="VYQ93_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV3336696.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERTSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            19786..20787
                     /locus_tag="VYQ93_002092"
     CDS             19786..20787
                     /locus_tag="VYQ93_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEV3336697.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            20784..21719
                     /locus_tag="VYQ93_002093"
     CDS             20784..21719
                     /locus_tag="VYQ93_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV3336698.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            21964..22080
                     /locus_tag="VYQ93_002094"
     CDS             21964..22080
                     /locus_tag="VYQ93_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEV3336699.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            22096..24015
                     /gene="tet(M)"
                     /locus_tag="VYQ93_002095"
     CDS             22096..24015
                     /gene="tet(M)"
                     /locus_tag="VYQ93_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEV3336700.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKINIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            24134..24301
                     /locus_tag="VYQ93_002096"
     CDS             24134..24301
                     /locus_tag="VYQ93_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEV3336701.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(24361..24714)
                     /locus_tag="VYQ93_002097"
     CDS             complement(24361..24714)
                     /locus_tag="VYQ93_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3336702.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         7707 a         4751 c         4747 g         7541 t
ORIGIN      
        1 gattctggat ggtttttaga ttaggggtga ataattgggg ataaactatc aggacaagag
       61 ttatttgaaa ttgtaattca aaaaattgaa gaatttcgag caattgtagg aactcagatg
      121 gtatttttag atagtatcaa tcatcctaaa gtcattcaat tttatgaaca gttcggattt
      181 gttgcttaca gtcaattgat taaagacgat catcaagtaa gttatcaacc tatggcatta
      241 aatatgtcac tctataaaaa ataagaatca tgtcacctaa actgatgaca tgattctttt
      301 atttcaccgt ataagcaaaa ctgatggcac tatctgcttg cgagattttt ctaaaggtat
      361 agttagggtt gccaccatag ttagtttcag acacaaggaa agaaccatca tcatagactt
      421 tctcgacaaa agccacatga ccgtagatag ctggtgtgcc atgtgtacct cctacaaaag
      481 aaacaatggc acctgctctt ggtgtagagc cagtttcccc accaagactt gaagctgtcg
      541 caacccagtc ttgaccattt cccatggtat taatgattga aatcttctca ccattacttc
      601 cttttaattt taagcctaac tgattcatac gagccgcaac accccatgta cattgtccat
      661 aggcataggc cataccatct ccaccaccag gaacagaatg atcatacaag tccccacgaa
      721 caccttcgag ggattgtgga tcactctttg cctgtcctcc gtttgtttgg ctaaagcctt
      781 tttcaatttg gtaataccat tctgttgctc tggtttgtct ttccagtagt ttgtcaccag
      841 aatttccctc ccaataggta aggaagagtt gggctaaatt ggctgcactg cccgtatttc
      901 caaagaaatc ctttaaccaa ctttgatagt aaggactatc cccatgaagc ataaaatcaa
      961 gttggagatc taaatcatac cacttcttat ttttgatatg tgcataattc aataaagctg
     1021 tatgccgtgt tgaaccatct gcagtatccg tccattgacc taaccccaaa cctctatgaa
     1081 ggatattagg ataagcacca ctataaatgg ctggacctcc aattgctaac caggtttcat
     1141 catcccatga ggaatcggta gcgccaacag gaggagataa ataatcccct tcagctcgtt
     1201 ttggattaat agaagattct accgaccaat ttcctaaaat agccgcaatg gcttggggac
     1261 ttgccccttg agatttcaaa aactcataaa tatgttttgc tcgttcaaac tcatccccac
     1321 caaactgacc aatggcaggt aagatagttg tctgaagttg aatgactttt ggaaaataaa
     1381 attgcggatt gacatacacc aatttttctt tcttgttctt atacttttga taggaaactt
     1441 tcaaacctgt atcgtctggt gtttcaccaa taatatcacc cgttaagact cttgtcccct
     1501 caatcgcacg gccattatga atagaataca aggtcaatcg actctcattc tctccttttc
     1561 cgttagtgag aataatattg tcaccatcta gagatacaac tccatccatt ggtgcgacaa
     1621 tcgtttggtg agcctttgct tctagtagaa tgtactcctg aagagtaggt ttcccatcta
     1681 aatcatagta tccataacga taagtcatgg ttagactatc ttcgttgctt ttcccctcaa
     1741 atggattgtc caattcctgc atggaagcat atacaccctc ttcttttagt tccttcattt
     1801 cctcttgatc gtctttcgag agtttatact taggagtttc atagaggtct tgcatggatt
     1861 tcaaatcatc cccatcgttt aaatcatgcc acaaagtaga cagataatcc ttgtaagttt
     1921 ctgaactaaa taagtgaact ggtttgtgta actcatagtc atggaattta aagttcatat
     1981 agcccatcac atcatcaact tttgtgtaat aagtaattcc tttgtcattt gtgcgagtat
     2041 gttctgcatc ttcccaagtt agatgggtat aagcttttgt taattcaaat tcatcttgtt
     2101 gaatcaaact agcagatgaa aatcctaaaa agaagctcat cataagtaag agaagaaaga
     2161 ctaatcctcc aactatccag gttgcaggat tcccagccgt aaatgtaaag aaggaaaagg
     2221 ctgcttttag tttttgatag atatttcgga cacttgaaag accttgtttc tttaattttc
     2281 gataccgatt tttaaaggaa cttggattat ctttcgctag tttccatcct tttccatcct
     2341 taaaatgatg gtatcgctct tttgtgttgg tcagtctttt cttggtaaaa cgacctgttg
     2401 cttgtcctgt tttgacacta gcttttccaa gattataaga aagtcgactg tagcgtttcc
     2461 cttttctaat ggtctcttga agtgtacgat agccttctaa atcttcattt tctgaagcta
     2521 actctccacc ttcacgtcca aggacataaa gaaaagtttt ggctttccta ctgacttttt
     2581 tggacttata ggcttgttta gtagatttta gattatcttt tgcggccttg acttctttct
     2641 tagcttttaa ttcttctaaa ctcttccctt gaaagaaaaa attggatttt gctttcgatt
     2701 cctgaccata aagaaatttt tgattggttt ttctttcttt acgactctct tttctttctt
     2761 cttttgcttc taccttggct tctctaaatt gcttcttggc tattttcaat cgtttcctag
     2821 catgaggcaa tcgtctgtct cttaattctt tccgattcaa aagagatgga ggagtatttt
     2881 gaaggatgtg attgtagtct tcatttgctt gttttactct agcctttgaa gcttctctca
     2941 tctcctctag cttttctttt atctcttttt tccatgcttt ttcatccagt acagcggaat
     3001 cttttttctg tttccttacc tccttctttc cttttttcaa gaatttcttc tcatctttta
     3061 gacttcttct aaatgccttt cgggcacgta tgatttctct tttatccttc atttaccttc
     3121 cccttaatta gaagccattt tatcaggatc cgtactcatg atatcaaaca attgagtacc
     3181 ttgaggaatc ttatttttaa agggaacgac aactgaacca gcttttatta gtccagcccc
     3241 tttttctgga ttgactaggt atttttcgag ttcttttgac aagcctaaga gttgaaccaa
     3301 ttcttctcta tcattttttg cttgcttgag gagaatcata aattcactat ttgcaataat
     3361 ccgtctacca tttggatcta acaataaggt ttcgacgttt tgggttatcc cagtcggact
     3421 ggctccatat tttctgacac gactccacaa tttaaagaag aaatcactgg catatttatc
     3481 taataagaga agctgcatct catcgaaata aatccaggtc ttcttcccta atttttgatt
     3541 ccgaacgaca cgattccata tctgatcaaa aacaaccata agggcaattt gtttcagctc
     3601 atctcctaac tttttaacat tatagattaa gaaattggac cctgtttgaa tattggtctt
     3661 atgagaaaaa atatcaagag aaccttcaac atacagttcc atatcaagtg ccaaattctg
     3721 cgcttcttct tctgcttgtt ggctcaagac aaagacccat tcttccaaag aaggctcttt
     3781 aaatgactga taagtgagtc tcgtaactcg gtcgataatc gatttttctc tcccatccat
     3841 ttttctatcc aataacttac cgataaagga taaaagaaat tctgatttta cctttacagg
     3901 atcctcatcc atattttcct cagacaagtc aaggacattg agataggttt gggaatcagg
     3961 cgcaatatca atcatttctc ccccaaaagt tcgtccaatg acactgtact ctgcttctgg
     4021 atccacgatg ataatttcag tattttcacc agattccttg attttggtcg tgataatttc
     4081 atgcttggtt gccatccctt tcccagctcc agatgttcct aaaatcagac cagacggtgt
     4141 atttaatagg ctgcgatcaa tggtaataat attacttgat atttgattga taccgtaata
     4201 tttcccacta cggtcttgta agtctactga agtccaaggt gagttcactg ctacattaga
     4261 tgttaataaa ctccgtgata ctccctctaa aaaatcacaa ccaaatggta gcaaactatt
     4321 aaaggctgct tcttgcatat atggtagttt atcaatcatt aggtcatttg agccagccac
     4381 ttgttgaata gtgtctagtg cttgtttgag ttcttcttca tcctgaccaa aaacaccaat
     4441 caagaagact gtttgaaata gtttatctcc tgtctcggtc atggttttta agagttcctc
     4501 agcttcatcg atattgcttt ctaatacatg acctactttt tccaaataga tacctgtacg
     4561 agcaagtttt tgttgttccc cgatcttttg ggattccatc aaggtcttct ttgttcgtag
     4621 tttcttcatg gcatctgcct tggtcgaact ttgagcatgg aggcttacaa tcaattccaa
     4681 atctccttgc atgaggtctc tgataaactg atctcctaat tccataccgt agtctctcac
     4741 atagacaatc tgtaataaac ggtcattgat ttgtaggtaa ttcttgtttt taaaatccaa
     4801 gagattaggt gctatgaagt gacgagttgt ctgacccgat ctcgttaaat cacggtaaga
     4861 aaaaggaaga tggtgttctc ctctaagcat atcggccaac aagttcaccc gttcttctcc
     4921 agccaaggat tcaaatcgtg catcaatttc tgagaaacca ctcttgaaat attcgcctat
     4981 ttgagacaag gaacgatagg cttgtttggg attagaatcc tttctaccaa aactaatcag
     5041 tttcacagct gaaaaattat tttcaccact gtctaaattc tgattcatca tccgattcaa
     5101 ttctttacga tagctatcat acccatcttc tttttcctca tacagaacac tctgtctaaa
     5161 cttttctaaa ttcaatcttt tattaaagat agtcaattgg aagttggttt ggtcatctag
     5221 tgagttaatc aaatcagaat acttctcaat gattgcgccc ttatcttcta aaccaacggt
     5281 ctggtaattg acatcaccaa gtaaatagct ttgtgagaaa taatcttctt ttacctgcat
     5341 cagaccattt tgatacaggg cttgatagga aagagtatta gccgttgagg gtaacacttc
     5401 ctcttttttc cctttaactt cttccttttt attagtcatt gaagtttttt gtttctttaa
     5461 tgtatttgat tttcttttca tgttcaggtc ctttctttcc tgtaattgtg cgtaggggaa
     5521 ccgttaattc aaaatgaaga cggtatttca aataatgttc aaaatataaa tcattgggtt
     5581 tatagactcc aaaaagcatt agggggatgg taaaagcaaa cacaaaaccg taaacaaacc
     5641 aatctccaaa ttgccagaaa aagagattca agcccaaaac aataattgtg acaataaagg
     5701 ctggtaaaac aaagatgatt tgtcttgtgg tgaaacctaa ccaagccctg tgttggtatt
     5761 ttgagatgtc tttaaagaca catgtattca tgactttcct ttctaaaaag gctaagaagc
     5821 aatcacttcc tagcctttat ctaattacat acctaagatt gagcgagccg tacgttgaga
     5881 accaacgagg gcaataatca gtaagatagc ttgtactaaa ctaccaaaca taatcgcaag
     5941 tgattgcaag actcctgcac catttgaaac agctattttc ccagcagatt caaacaaagg
     6001 aacaagagaa acaatcagaa aaatgagaac cccttgtacc gcatagacca taatattttt
     6061 taaatagcca ataccaatag acttccattc atcacttaaa aatgttggaa tcgtaagagg
     6121 ggcaaatggg atcataaggt agagttgaat aaatcgaata gataccaaaa gattaaccat
     6181 ggctgcactt actatccgaa caagccagat aaggagagcg aaaaagccca caatcatccg
     6241 gccaataaat cctgaacctt ttaatccaga gagtgtatca tacttcgccc caccgtgagc
     6301 cacaatcgag gccacttgtt cgatggcgtg actcgcaatc ccgatgatag cttctactat
     6361 aacggtagta ttggtaatga ccactgcgac cataatataa ctaatcaaca tcggcgctaa
     6421 tgcttcaaag gtcatcgctc caccagagtt agcaatcttc tttgccatct tcgaaaattc
     6481 taagatgaga acaactgata aaatcgcaac tccaagaggc tgcatgacac ttttagtaat
     6541 actagacata taagtccaaa ctgttggatt gtagctagat agggatttaa tcagatctac
     6601 cgtagattgt aaatccacat taaatccttc aaataaattt tcagctgata ttttttcaga
     6661 tgcaaggtaa acaaagggtg agactaaact aagattcatg tcattgttta tcctcctaaa
     6721 ttgaaatctg ggttacaaag gctccagcag cccctaccat aacaccaccg acaatttcaa
     6781 gaatggcatt ccgaacacct ggtccaccat ctttaatgtt ggttgcaagg ttgacaatcc
     6841 ccacaacaac gagaaaggca ccaactgcaa tcaatccctt ctgtaacaaa gacatagctt
     6901 gtgcaaacat agcacttgcg tctactccat aaacaaaacc tttaaaatgc gtaatcatct
     6961 atttcctctt ttctattttt attttaaact agattcaaaa gttaaatcac gaattctaag
     7021 gtcgtcaaga taattttctt gtctttgatt caaaggattg atttgatagt tccaccaccg
     7081 ttcatcgctt tcttgattag ctaggtactt ccagtttgga tgcttagtgg aattgtattt
     7141 tttgctttta aagacaggca tattggcaat tcgaaccaag cattcatgcc gtttcatatt
     7201 tccgacctca tcgggtgtca ttaagtcacg agcaatcttt tgatgagaaa gggatcctga
     7261 acctgtttgg ccaaaggaac gactagtatt tcgaacatca atggtttgtt taccgagtaa
     7321 gccactcata aatttaaagg tatcttcatc attaccacct aagtagacta agctatcaca
     7381 gttccccaaa atggttttcc aagcttcttt ttctttatag agcccttgaa gttgggcaat
     7441 attttgaaga ataggaacga gactcatatt tcgagaacgg actgttgagg tttgttcagc
     7501 aaaatctgga atttctccga tatttgcgaa ctcatctaag taaactctca catgaagagg
     7561 caattgaccc ttaaaatcaa tatctgcttg tcttgttagg gtttgaaata cggttgaaaa
     7621 aaagagggct gaaagaaagc gaaaggtact atcgttatct gggataacta agtaaaccat
     7681 tgatttttcc ttgccccatg tcttcatatc aagggtatct cttttggtca agtccatgac
     7741 actttgaata ttgaagaggg caaatttagc agtggttaca gctataacag aatccagagt
     7801 cttatcctta taattttgaa aatctgccca atttcgcatg gtaaaatttt cagtcccata
     7861 ctttttagca taattttcaa atagaatttc taagacactt ttttcctggt tttcaccctt
     7921 ggataagtgt ttaatgagtt ttgagatttc agcaaaactt ggataacgac ctcgtttttt
     7981 tcgctcttcc acttcttttt tttgacgttt caacaagttt tgatattcct tttgacttaa
     8041 acgactttct tctatgagct gttctcttgt tttaggtgga ttatagaaat cgactaagta
     8101 agaggctaaa gctcgaacca aagtcataga agcttcatcc caaaatggat cacttcggga
     8161 gccagagcct ttggtgttat taaaataaac agtcagcatg cgattcaaat cgttttctgt
     8221 ttctatatag cgaaaaggat taaagccatc tgagttcttc atattgacta aatctaacac
     8281 ctttacttgg tagccatgtt ctaaaaagag tttgcctgtt ttctcggcca agtgatcttt
     8341 aggatccact acaatattag aactattcat ctgaatcaga ttgggtttca caaagcgaaa
     8401 tgtcttccca cttcctgaac ctccaatcac cgcaatattc ttatttctat catattgggg
     8461 tggtttttta tctaataatg tcaaacgaac atcttgtgct aagatcgtat catgagaaaa
     8521 ttccttaccg taaaagagct tcttttcttt tagagttcca aaacgggcgc tcccgtattc
     8581 taccccttct cggtattgtt ttttaccagt ctctagatag agataaacca gtaacatcat
     8641 cacaaaacct agtagaaaaa aagcacttga ttttccagta aaagaaacat tccatggcga
     8701 ctgaagaact tcatcttgac cttccatcag aagatgagtc catttatcta aggtatttcc
     8761 agtataggaa tcatacaaaa gcgtcaaacg atgaaaaaga tagcctagta agatacctaa
     8821 cagtgagaat agtaggaatt tttttccact gtacatcatc tcaccatctc tttctgtttg
     8881 acggctcctt cttgtctaaa ggtaatttgg gatttagctt catcaatagc atcatctaat
     8941 gacttatcca tggtaaaatc agctaatttc tccggatcat taaccatttt ttctaaaaga
     9001 tggtctaaat gattgtctaa aatcgaacgg tctttcgcgt agaaatgtag agaatcccct
     9061 tgccaagcga tggctaaagg aatctcttct ttttctaaaa aagctttaaa tttctctata
     9121 tcaattggtt tgtctaaaaa atcttttttc agattaatcg tatcaatcgc atagggagat
     9181 tgtagcaatt cttctaattt ctgcactcct atcttatagg cggaatcctg tgctaaagcc
     9241 tgacgtctag accattctag aatctttaaa agacttttta cagtaaataa aagactacgc
     9301 tctgcatatt gaactgccat tcgttcctgt tgttcagagg acatctgatg cctccttctt
     9361 cacaaatagc agctttcctt ctttataacg ataagctatc aatttctgac gttgcttaat
     9421 cgacttgaca accttcagta gatctctcga ataaggttct cgaagagtga cttctactgc
     9481 cttcccatca acgataccaa cacgtttaaa ttcaatgtag tcttttttga gatgtcctaa
     9541 gcccatacca aaaggaaagt gatgaatcaa ttggatggta caaccacctt tatttcccat
     9601 ttgtttcaaa tcatacaagt ttactacctt catgattaac tcctttatct agtttgtcgc
     9661 atcgtttaag tctggtaacg ataaactccg tgtctgttag aaaattctca cacacgtctt
     9721 gtgccagttg cccttcacag ggaaatactc tcagtcccta cttacacagg cacgctaatc
     9781 aagacggagt ggattcaatt ttcaaagaac aggtagcttt attatagaga agagtagttg
     9841 aaatttttat cacatttttt atttttctgt agagctaaat tcatactaaa caaagatttt
     9901 gaaataaaag actaacattg ttacataacg ttagcatttc atttccctaa aataagctaa
     9961 ttttgtctaa aaaatagcat aaaaatctag ttatccgcat aaaaactgga cttatcacac
    10021 tttatcaagg tcaaaaccac tcaatttact actaatttac tacttatgaa tgagctttga
    10081 tacgacgatt tatccttgaa aagtgaagat ataaagatac ttccaataaa atttgaatat
    10141 ttaataggta gacacttcaa aaaatgaggt gtctattttt ttacccgatt ttgaaaggaa
    10201 gtgaacttat gaaaacaaaa aatcaagaat caaaaggtcg ttccccactc tttaagacca
    10261 tcaaacattc attcagccaa taaaaaagaa aggataggta aaaatatgga acttaaattt
    10321 gtgattccca acatggaaaa aacattcggc aatttagaat ttgctggcga ggataaagtc
    10381 gttcagcgaa gaatcaacgg acggctaact gtcttatcaa gaagctataa tctctattct
    10441 gatgttcaaa gagcagatga tattgtggtg gtgcttcctg ctgaagctgg cgaaaaacat
    10501 ttcggctttg aggaacgtgt gaagttagtc aatccacgta ttaccgcaga gggctacaaa
    10561 atcggcactc gtggttttac aaattacctt ttacatgctg acgacatgat aaaagaataa
    10621 agaaagagag gaaaaatgat gagattagca aatggcattg tattagataa agacacgact
    10681 tttggagaat tgaaattctc tgctctacgt cgtgaagtga gaatccaaaa tgaagacggg
    10741 tcggtttcag atgaaatcaa ggaacgtacc tatgacttaa aatccaaagg acaaggacgc
    10801 atgattcaag taagtattcc tgccagcgtg cctttgaaag agtttgatta taacgcacgg
    10861 gtggaactta tcaatcccat tgcggacacc gttgctactg ccacctatca aggagcagat
    10921 gttgactggt atatcaaggc agacgatatt gtgctgacaa aggattctag ttcattcaaa
    10981 gctcaaccac aagcaaagaa agaaccgaca caagacaaat agtcgctagg tagaaaggag
    11041 actttttcgc atgaaacagc gtggtaaaag gattcgccca tctggtaaag atttagtctt
    11101 tcattttacg atagcgtcac tcctgcctgt tttcctgctg gttgtcggac tgtttcatgt
    11161 gaagacaatc cagcagatca actggcagga ttttaaccta tcacaagcag ataagattga
    11221 cattccctat ttaattatca gtttcagtgt cgcaattctt atctgcttgc tggtagcgtt
    11281 tgtattcaaa cgggttcgct atgatacggt taaacaactt taccaccgtc aaaaactggc
    11341 aaagatgata cttgaaaaca agtggtatga atctgaacag gtcaaaacag agggtttctt
    11401 taaagatagt gctggtcgta caaaggaaaa gataacctac ttccctaaaa tgtattatcg
    11461 acttaaaaat ggcttgatac agatacgggt ggaaatcacg ctgggaaaat atcaagacca
    11521 actcttacac ttggaaaaga aattagagag tggcttgtac tgtgagctga cggataaaga
    11581 gttaaaggat tcctatgtgg aatatacttt gctctatgac accatagcca gtcgtatttc
    11641 tattgatgaa gtagaagcta aagatggtaa acttcgctta atgaaaaacg tatggtggga
    11701 atatgataag ctccctcata tgttgattgc tggtggtaca ggtggcggta aaacttactt
    11761 tatactgaca ctgattgaag ccttgcttca tacagattca aaactgtata ttcttgaccc
    11821 gaaaaatgct gaccttgcgg acttaggttc tgtgatggca aatgtctact atagaaaaga
    11881 agacttgctt tcttgcattg aaacattcta tgaagaaatg atgaaacgta gtgaggaaat
    11941 gaagcagatg aagaactata agactggcaa aaattatgct tacttaggtc tcccggcaca
    12001 cttcttaatc tttgatgaat acgtcgcttt catggaaatg ctgggaacaa aagaaaacac
    12061 cgcagttatg aataagctga aacagattgt catgttaggt cgtcaagctg gcttctttct
    12121 aatactggct tgtcaacgtc cagacgcaaa atatttaggc gacggaatcc gtgatcagtt
    12181 taatttcaga gtggctttag gtcgtatgtc tgaaatgggc tatggcatga tgtttggcag
    12241 tgacgtacaa aaggatttct tcttaaagcg aatcaaaggt cgtggctatg ttgatgtagg
    12301 aacaagtgtc atatcagagt tttatactcc ccttgtacca aaaggatatg atttcttgga
    12361 ggaaattaaa aagttatcca acagcagaca gtccacgcag gcgacgtgcg aagcggaagt
    12421 cgcaggtgtg gactgatctt gctggctggt gtggcaatag ccacgccagc acttaacccc
    12481 ccgtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
    12541 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
    12601 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
    12661 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
    12721 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
    12781 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
    12841 ttatgtaagg attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
    12901 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
    12961 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
    13021 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
    13081 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
    13141 cattaacgat aagacaggga ttttgaatat ccctgtactc actgaaaagt gccaacagga
    13201 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
    13261 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
    13321 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
    13381 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
    13441 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
    13501 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
    13561 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
    13621 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
    13681 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
    13741 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
    13801 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
    13861 tatgaatttt ggacaaaacc tttataactg gtttctatca aacgctcaat cactggtgct
    13921 tttagcaatc gttgtgattg gcttgtatct tggcttcaag cgtgagttta gcaaactgat
    13981 tggcttttta attattgcga ttattgcggt tggcttagtc ttcaacgctg ctggagtaaa
    14041 agacatttta ctagagctat tcaatcgcat tattggtgct taaataaaac cgttcttttg
    14101 tggaatataa gtggttttct tatgttccgc aaaggaatgg tacaccaaac gaagtgcggt
    14161 agggattttt gaatctctac aaagaaagga cgtgaatata tggacgatat gcaagtctat
    14221 attgcgaatt taggcaaata caatgagggc gaattggtcg gtgcgtggtt tacctttccc
    14281 attgactttg aggaagtcaa agagaaaatc ggcttgaatg atgaatatga ggaatacgcc
    14341 attcatgact acgagttacc ctttacggtt gacgaataca cttccattgg cgaactcaat
    14401 cgactatggg aaatggtatc ggaattaccc gaagaattac aatcggagct atctgctctg
    14461 ctcactcatt tttcaagcat tgaagaacta agcgaacatc aagaggatat tatcattcat
    14521 tccgattgtg atgatatgta tgacgtggca cgctactaca ttgaagaaac gggtgcttta
    14581 ggcgaagtac cagctagtct tcaaaactat attgattatc aagcctatgg tcgggattta
    14641 gacctttcag gaacgtttat ctcaaccaat catgggattt ttgaaatcgt ctattaaatc
    14701 tgtcggtaca ttactactgg cagattttct attttacggg gtggctcaat cagctacccc
    14761 tattttttat gaaaggattg attacatgaa gaaaatacga agctatacca gtatctggtc
    14821 tgtggaaaag gtactgtatt ctatcaatga ttttagactt ccgtttccca taacctttac
    14881 gcaaatgaca tggtttgtcg tgtcactctt tgcagtgatg atacttggca acttgccccc
    14941 tctttccatg atagagggag catttctcaa atactttggg attcctgtgg ctttcacatg
    15001 gtttatgtct acaaaaactt ttgatggtaa aaagccttat ggatttttga agtctgtcat
    15061 tgcttatgca ctgcgaccaa agctgaccta tgcaggaaaa aaagtaacgc ttggcagaaa
    15121 ccagccacaa gaagccatta cagcagttag gagtgaattt tatggcatat ccaattaaat
    15181 acattgaaaa caatctcgtc tggaataaag acggggaatg ttatgcttac tatgagcttg
    15241 ttccttacaa ttactcattt ctaagtccag aacagaaaat acaagtgcat gattctttca
    15301 gacagcttat cgcacaaaat cgtgatggca aaattcatgc tttacaaatc agtacagaat
    15361 ccagcatacg ttctgcacaa gagcgttcca aaaatgaagt cactggcaag ctcaaagcgg
    15421 ttgcctatga caaaatcgac caacagacag acgctttaat atccatgatt ggcgaaaatc
    15481 aagtgaacta ccgtttcttt atcggcttta agttgcttct caacgatcag gagttttcta
    15541 tgaaaagtct taccgttgaa gcaaaaaatg ctttgtctga ttttgtctat gatgtgaacc
    15601 ataagctgat gggcgatttt gttagtatga gtaatgatga aatcctgcgt tttcagaaga
    15661 tggaaaagct cttagaaaat aaaatctctc gtcgtttcaa aatccgcagg ttagataagg
    15721 acgacttcgg ctatctgatt gaacaccttt acggacagac aggcactgcc tatgaagagt
    15781 atgagtacca tctatcaaag aaaaagctgg ataatgaaac gctgattaaa tactatgact
    15841 tgattaagcc tactcgctgt ttggtggaag aaaaacagcg atatttgaaa atccagcagg
    15901 aagatgaaac cgtctatgta gcttacttta ccattaacag cattgtcgga gaactggact
    15961 tcccgtcctc tgaaatcttc tactaccagc aacagcaatt tacattcccg attgatacgt
    16021 caatgaatgt ggaaattgta gcgaatcgta aagccctatc tactgtccgc aataaaaaga
    16081 aagaactgaa agacttggat aaccacgctt ggcaaagtga taatgaaacc agctccaatg
    16141 tggcggaagc tctggaaagt gtgaatgagc tggaaaccaa tttagaccaa agcaaggaat
    16201 ctatgtacaa gctgtcttat gtggtaaggg tatcagcaaa tgatcttgac gaactcaaac
    16261 gtcgttgtaa tgaagtgaaa gatttttatg acgatttaag cgtaaaactg gtacgaccat
    16321 ttggggatat gctcggctta catgaagaat ttttacctgc cagcaagcgt tatatgaatg
    16381 attatattca atacgtgacc tctgatttcc tcgctggttt aggttttggt gctactcaaa
    16441 tgctggggga aaatgagggg atttatgttg gctacagctt agatactgga cgcaatgtct
    16501 atctgaaacc tgctcttgcc agtcaagggg ttaagggttc agtaaccaat gcgttagcgt
    16561 cggcttttgt tggttcgctg ggtggtggta aatcctttgc gaataacctt atcgtctatt
    16621 atgcggtgct ttatggggca caagcagtga ttgtagaccc aaaagcagaa cgtggcagat
    16681 ggaaagaaac cttgccagag atttcccatg aaatcaatat cgtcactctg acttctgatg
    16741 agaaaaacaa aggcttactt gacccttatg tgattatgaa aaatcccaaa gattctgaat
    16801 cactggctat tgatattctg acattcctta cggggatttc ctctcgtgat ggggaacgct
    16861 tcccaatcct tagaaaagcc attcgtgcag taaccaatag tgaagtacga gggttgatga
    16921 aagtgattga ggaattacgg gttgagaata cgccactaag taccagtata gccgaccata
    16981 tcgaaagttt tacagactat gactttgcac atttattatt cagtaatggt tatgtggagc
    17041 agtctatcag cttagaaaaa caactgaaca ttatacaggt tgcggacttg gtacttcccg
    17101 acaaggaaac ttcctttgag gaatatacca ctatggagct tttatccgtt gctatgctga
    17161 ttgtcattag tacctttgct ttagacttta tccatacaga ccgaagcatt ttcaagattg
    17221 tagatttaga cgaagcatgg agctttttac aggtagcaca aggaaaaaca ctatctatga
    17281 agctggttcg ggctggtcgt gctatgaacg ctggggtata tttcgtgacc caaaatacag
    17341 acgacctctt agatgaaaaa ctgaaaaata acctcggctt aaaatttgca tttcgttcca
    17401 ctgaccttaa cgagattaaa aagaccttag ccttttttgg tgtagaccca gaggacgaaa
    17461 acaatcagaa gcgattgcgt gatttggaaa acgggcaatg ccttatcagt gatttatatg
    17521 gtcgtgtcgg tgtgatacag ttccaccctg tatttgaaga actgctccat gcctttgata
    17581 ccagaccacc tgtgcgaaaa gaggtgtaaa tgtgaaacca tcaatagtaa acagaataaa
    17641 atcaaactgg acgctgaaac gtctaggtaa agtggcaatg acagtggctt tcacacttgt
    17701 gattgccatt tttcttttag ccatgctggg aacggtggtt caagctgcgg gcttggtaga
    17761 tgatacggtc aatgtggcaa atgaatacag ccgataccca cttgaaaact atcaactgga
    17821 tttttatgtg gataatagct ggggctggct tccgtggaac tggtcggacg ggattggaaa
    17881 acaggtcatg tatggactat atgccattac caattttatt tggacaatca gtttgtatgt
    17941 ttccaatgcg acaggttact tagtacagga agcctattcc ttagacttca tttccgctac
    18001 agcagattcc attggtaaga atatgcagac cttagctggt gtgagtgcaa acggattttc
    18061 aacagagggt ttctatgttg gattcctctt actcttgatt ttggttcttg gggtttatgt
    18121 tgcctatacg ggactgataa agagagaaac cacaaaggca attcatgcca ttatgaattt
    18181 tgtgctggtg tttatcctat cggcttcctt tattgcctac gctcccgact acattaaaaa
    18241 aatcaatgac ttttcatcag acatcagtaa tgccagttta tcacttggca cgaagattgt
    18301 catgccccat tccgatagtc aaggcaagga cagcgtggac ttaatcagag atagcctgtt
    18361 ttccatacag gttcagcaac cgtggctact gcttcaatac aacagttcag acattgaaag
    18421 tatcggtatt gaccgtgtgg aaagcctgct ctccaccagc ccagattcca acaatggcga
    18481 agacagagaa aaaattgttg cggaagaaat tgaagacaga agcaatacca atctaaccat
    18541 tacaaagacc attaaccgtt taggtacagt cttcttccta tttgtcttca atattgggat
    18601 ttccatattt gtattcctat taacaggaat catgattttc tcgcaggtac tttttatcat
    18661 ctatgctatg tttctgcctg tgagctttat tttaagcatg attccatcat ttgatggtat
    18721 gtcaaaacga gccataacaa agctctttaa taccattttg acacgagctg gaatcacatt
    18781 gattattacg acagcattta gtatttcaac catgctctat accttatcgg ctggttatcc
    18841 gttctttttg attgcttttc tacagattgt gacctttgca ggaatctact tcaagctggg
    18901 cgatttaatg agtatgtttt ctctacagag taacgattct caaagtgtgg gaagtcgtgt
    18961 gatgagaaaa cctcgtatgc ttatgcacgc tcacatgcac cgtctacagc ggaaacttgg
    19021 acgttccatg actactctag gggctgggtc tgccattgtt acaggtaaaa aaggacagtc
    19081 gggttcgggg agttctgcaa ggacacaagc agatcactcc cgaccagacg gaaaggaaaa
    19141 atcaacactt ggaaaacgta tcggtcaaac catcggtaca gtagctgata ccaaagacag
    19201 aatggtagac actgctagtg gtttgaaaga acaggttaaa gatttgccga ccaatgcaag
    19261 atatgcagta tatcaaggaa aatccaaagt aaaagagaat gtccgtgatt taaccagtag
    19321 tatttctcaa accaaagcgg acagagccag tggacgcaag gaacagcagg aacaaaggcg
    19381 aaaaaccatt gcgaagcgtc gctctgaaat ggaacaggtc aaacagaaaa aacagcctgc
    19441 ttcttctgtt catgaaagac cgactacaag acaagaacaa tatcatgatg aacagacctc
    19501 aaaacagtct aatattcaga cttcatataa ggaatctcaa caagccaaac aagagcgtcc
    19561 agcagttaag tccgattttt caagtccaaa agtggaacgc caaggcaata ccgttcaaga
    19621 aaaaaccgtt caaaagccag caacttcaac cactacagca gatagaactt cacaacgtcc
    19681 aatcacaaaa gaacgtacgt ctactgttca aagagtacca ctacaaaata caagaagtag
    19741 accaccaatc aaaaccgcca ccattaagaa agtcggtaag aaaccatgaa gttgaaaact
    19801 ttagtgattg gtggttctgg attattcttg atggtcttct cactgcttct gtttgttgcc
    19861 attttatttt cagatgaaca ggacagcgga atttccaata ttcattatgg aggtgtgaat
    19921 gtttccgcag aagtgctggc tcataagcct atggtagaaa aatatgccaa agaatatggc
    19981 gttgaagaat atgtcaacat acttcttgcg attatacagg tggaatcggg cggtactgcg
    20041 gaagatgtta tgcagtcctc ggaatccctc ggtcttccac ctaattcatt gagtacagaa
    20101 gaatccatta agcaaggtgt gaagtatttc agtgaattat tagccagtag cgaaaggctc
    20161 agtgtagatt tagaatcggt tatccagtcc tacaattatg gtggtggttt cttagggtat
    20221 gtggctaatc gtggaaataa atataccttt gaactggctc aaagtttctc aaaagagtat
    20281 tcaggtggcg aaaaagtgtc ttaccccaat cccatagcca tacctatcaa tgggggctgg
    20341 cgatacaact atggcaatat gttttatgtg caactggtaa cgcagtatct tgtcacaaca
    20401 gagtttgatg atgatacggt acaagccatc atggacgaag cactgaaata tgagggctgg
    20461 cgatacgttt acggtggagc ttccccgact acttcttttg attgtagcgg actgacacaa
    20521 tggacgtatg gaaaagctgg aattaactta ccacgaaccg cacaacagca atatgatgtg
    20581 acccagcata tcccactatc ggaagcacaa gctggcgatt tggttttctt tcattctacc
    20641 tataacgctg gctcttatat tactcatgtt gggatatacc ttggcaataa ccgtatgttt
    20701 catgcaggcg acccaatcgg ttatgccgac ttaacaagcc cctactggca acagcattta
    20761 gtgggagcag gacgaatcaa acaatgagaa aggaagattt aatgatgaaa tttagaaaaa
    20821 atcagaataa agaaaaacag ataccaaagg aaaagaaacc tcgtgtctac tataaggtca
    20881 atcctcataa aaaggttgtg attgccttgt gggtactttt agggcttagt ttcagctttg
    20941 cgatattcaa gcactttaca gctatagata ctcatactat tcacgaaaca actatcatag
    21001 aaaaggaata cgttgatact catcatgtag aaaattttgt agagaacttt gcgaaagtct
    21061 actattcatg ggagcaatcc gataagtcca ttgataatcg aatggaaagt ctaaaaggct
    21121 atctgacaga tgaacttcaa gctctcaatg ttgatacagt acgcaaagat attcctgtat
    21181 cgtcttctgt aagaggattt cagatatgga cggtagagcc aactggcgac aatgagttta
    21241 atgtaaccta cagtgtagac cagctcatta cagagggaga aaatacaaag accgtccact
    21301 ctgcttatat agtgagtgtc tatgtagatg gttctggaaa tatggtactg gttaagaatc
    21361 cgaccattac caacatacct aagaaatcaa gttataaacc aaaagccatt gaaagtgagg
    21421 ggacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg ttcttcaagc
    21481 tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg atattaaaac
    21541 caatcggaaa agagtacatc tttcaagaac tggtaaatcc tattcacaat cgtaaggata
    21601 atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa gcaacgcagg
    21661 tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt atagaataac
    21721 aaatattggt acattattac agctattttg taatcacgta ctctctttga taaaaaattg
    21781 gagattcctt tacaaatatg ctcttatgtg ctattattta agtatctatt taaaaggagt
    21841 taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg gaaacaaata
    21901 attggatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga atacctttat
    21961 catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat ggttatgcat
    22021 aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt gggcttttga
    22081 atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt tgatgcggga
    22141 aaaactacct taacagaaag cttattatat aacagtggag cgattacaga attaggaagc
    22201 gtggacagag gtacaacgaa aacggataat acgcttttag aacgtcagag aggaattaca
    22261 attcagacgg cgataacctc ttttcagtgg aaaaatacta agataaacat catagacacg
    22321 ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt agatggggca
    22381 attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt atttcatgca
    22441 cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca aaatggaatt
    22501 gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat tgtaatcaaa
    22561 cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc tgaacaatgg
    22621 gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg taaatcatta
    22681 gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgttc tctgttccct
    22741 ctttatcatg gaagtgcaaa aagtaatata gggattgata accttataga agttattact
    22801 aataaatttt attcatcaac acatcgaggt ccgtctgaac tttgcggaaa tgttttcaaa
    22861 attgaatata caaaaaaaag acaacgtctt gcatatatac gcctttatag tggagtacta
    22921 catttacgag attcggttag agtatcagaa aaagaaaaaa taaaagttac agaaatgtat
    22981 acttcaataa atggtgaatt atgtaagatt gatagagctt attctggaga aattgttatt
    23041 ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact attgccacag
    23101 agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct
    23161 gaacagagag aaatgttgct tgatgccctt ttggaaatct cagatagtga tccgcttcta
    23221 cgatattacg tggattctac gacacatgaa attatacttt ctttcttagg gaaagtacaa
    23281 atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga actaaaagag
    23341 cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat tcacatcgaa
    23401 gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct tccgttggga
    23461 agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc atttcaaaat
    23521 gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg gaatgtgacg
    23581 gactgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac cccagcagat
    23641 tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg aacagaattg
    23701 ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc acgagcatac
    23761 aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa taatgaggtc
    23821 attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga tttaactttc
    23881 tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt tactaccggt
    23941 gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg atatatgttc
    24001 aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg ttaaataaga
    24061 tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggagatag ttattataaa
    24121 ctacaagtgg atattgtgtc ctgtatgtgg aaataaaaca cgattaaaga taagggaaga
    24181 tactgaatta aaaaaattcc ccctctattg tccgaaatgc agacaagaaa atttaattga
    24241 aataaagcag ttcaaagtaa ctgtgattac agagccagac gcaaagacgc agagccgata
    24301 aaatgagatt aatacaatct cattttatcg gctctttccg ttatgtatgg attcttttaa
    24361 ttagtcttcg atgtttcttg cttcgttgat accgctggct aaagattcca ttaaggatag
    24421 ttctttgtct gtaaagctat ccatgtattt ctctatctgt aatcgtcggg tgctttttac
    24481 caagttatta gcaggtaaga aaaattcatc aacggaaaca tgaagtaacg atacaaggtc
    24541 ataaagaact tgtatgctgg ggtgttgccc tttattttca atattagtta agtaccgtgg
    24601 gtcaatttca atcaatgctc ccacttgttc acgagttaaa cctcgtttca atcgagcttc
    24661 tttaatggct aaaccaaagg ctctaaaatc atatttatct tcttttttac gcatagtaga
    24721 ccacctctat acattttatt gttcct
//
